LOCUS       DAVMMR010000001        86786 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMMR010000001 DAVMMR010000000
VERSION     DAVMMR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 86786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.001"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(15..152)
                     /locus_tag="VYS74_000001"
     CDS             complement(15..152)
                     /locus_tag="VYS74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV3788114.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            complement(354..941)
                     /locus_tag="VYS74_000002"
     CDS             complement(354..941)
                     /locus_tag="VYS74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3788115.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
     gene            complement(1128..1496)
                     /gene="rplL"
                     /locus_tag="VYS74_000003"
     CDS             complement(1128..1496)
                     /gene="rplL"
                     /locus_tag="VYS74_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV3788116.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(1572..2072)
                     /locus_tag="VYS74_000004"
     CDS             complement(1572..2072)
                     /locus_tag="VYS74_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV3788117.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(2111..2246)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2322..3581)
                     /locus_tag="VYS74_000005"
     CDS             complement(2322..3581)
                     /locus_tag="VYS74_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV3788118.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(3883..5631)
                     /locus_tag="VYS74_000006"
     CDS             complement(3883..5631)
                     /locus_tag="VYS74_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788119.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSTEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(5633..7357)
                     /locus_tag="VYS74_000007"
     CDS             complement(5633..7357)
                     /locus_tag="VYS74_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788120.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(7415..8353)
                     /gene="msrB"
                     /locus_tag="VYS74_000008"
     CDS             complement(7415..8353)
                     /gene="msrB"
                     /locus_tag="VYS74_000008"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3788121.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(8648..9517)
                     /locus_tag="VYS74_000009"
     CDS             complement(8648..9517)
                     /locus_tag="VYS74_000009"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV3788122.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(9519..10805)
                     /locus_tag="VYS74_000010"
     CDS             complement(9519..10805)
                     /locus_tag="VYS74_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV3788123.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDHDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFTESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(10956..11693)
                     /gene="mecA"
                     /locus_tag="VYS74_000011"
     CDS             complement(10956..11693)
                     /gene="mecA"
                     /locus_tag="VYS74_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV3788124.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(11893..12927)
                     /locus_tag="VYS74_000012"
     CDS             complement(11893..12927)
                     /locus_tag="VYS74_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3788125.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(12956..14389)
                     /locus_tag="VYS74_000013"
     CDS             complement(12956..14389)
                     /locus_tag="VYS74_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV3788126.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(14405..15391)
                     /locus_tag="VYS74_000014"
     CDS             complement(14405..15391)
                     /locus_tag="VYS74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3788127.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(15393..16496)
                     /locus_tag="VYS74_000015"
     CDS             complement(15393..16496)
                     /locus_tag="VYS74_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3788128.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(16486..17331)
                     /locus_tag="VYS74_000016"
     CDS             complement(16486..17331)
                     /locus_tag="VYS74_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3788129.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(17610..18884)
                     /gene="psr"
                     /locus_tag="VYS74_000017"
     CDS             complement(17610..18884)
                     /gene="psr"
                     /locus_tag="VYS74_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV3788130.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(18881..19729)
                     /gene="pheA"
                     /locus_tag="VYS74_000018"
     CDS             complement(18881..19729)
                     /gene="pheA"
                     /locus_tag="VYS74_000018"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3788131.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSIHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(19726..20202)
                     /locus_tag="VYS74_000019"
     CDS             complement(19726..20202)
                     /locus_tag="VYS74_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV3788132.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(20195..21478)
                     /gene="aroA"
                     /locus_tag="VYS74_000020"
     CDS             complement(20195..21478)
                     /gene="aroA"
                     /locus_tag="VYS74_000020"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV3788133.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(21570..21908)
                     /locus_tag="VYS74_000021"
     CDS             complement(21570..21908)
                     /locus_tag="VYS74_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV3788134.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(21919..23022)
                     /locus_tag="VYS74_000022"
     CDS             complement(21919..23022)
                     /locus_tag="VYS74_000022"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV3788135.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(23032..24198)
                     /gene="aroC"
                     /locus_tag="VYS74_000023"
     CDS             complement(23032..24198)
                     /gene="aroC"
                     /locus_tag="VYS74_000023"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV3788136.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(24208..25275)
                     /locus_tag="VYS74_000024"
     CDS             complement(24208..25275)
                     /locus_tag="VYS74_000024"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV3788137.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(25294..26148)
                     /locus_tag="VYS74_000025"
     CDS             complement(25294..26148)
                     /locus_tag="VYS74_000025"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV3788138.1"
                     /translation="MKLDGYTRLTAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(26138..26815)
                     /locus_tag="VYS74_000026"
     CDS             complement(26138..26815)
                     /locus_tag="VYS74_000026"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV3788139.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEASAPGQISLASMKKI
                     REILDEA"
     gene            complement(26812..27975)
                     /locus_tag="VYS74_000027"
     CDS             complement(26812..27975)
                     /locus_tag="VYS74_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV3788140.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVTFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSQELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(28662..30296)
                     /locus_tag="VYS74_000028"
     CDS             complement(28662..30296)
                     /locus_tag="VYS74_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788141.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLVNLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(30277..31026)
                     /locus_tag="VYS74_000029"
     CDS             complement(30277..31026)
                     /locus_tag="VYS74_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788142.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPAQSLESIYLSLAGRKEEVADASQGH"
     gene            31205..32659
                     /locus_tag="VYS74_000030"
     CDS             31205..32659
                     /locus_tag="VYS74_000030"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV3788143.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(32749..35367)
                     /gene="alaS"
                     /locus_tag="VYS74_000031"
     CDS             complement(32749..35367)
                     /gene="alaS"
                     /locus_tag="VYS74_000031"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV3788144.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(35389..35874)
                     /locus_tag="VYS74_000032"
     CDS             complement(35389..35874)
                     /locus_tag="VYS74_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV3788145.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(36149..36388)
                     /locus_tag="VYS74_000033"
     CDS             complement(36149..36388)
                     /locus_tag="VYS74_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788146.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(36649..37719)
                     /locus_tag="VYS74_000034"
     CDS             complement(36649..37719)
                     /locus_tag="VYS74_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3788147.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(37716..38489)
                     /locus_tag="VYS74_000035"
     CDS             complement(37716..38489)
                     /locus_tag="VYS74_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788148.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(38486..39292)
                     /locus_tag="VYS74_000036"
     CDS             complement(38486..39292)
                     /locus_tag="VYS74_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788149.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(39273..40430)
                     /locus_tag="VYS74_000037"
     CDS             complement(39273..40430)
                     /locus_tag="VYS74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788150.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(40590..41495)
                     /gene="murB"
                     /locus_tag="VYS74_000038"
     CDS             complement(40590..41495)
                     /gene="murB"
                     /locus_tag="VYS74_000038"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV3788151.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(41607..42359)
                     /locus_tag="VYS74_000039"
     CDS             complement(41607..42359)
                     /locus_tag="VYS74_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV3788152.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(42369..43100)
                     /gene="budA"
                     /locus_tag="VYS74_000040"
     CDS             complement(42369..43100)
                     /gene="budA"
                     /locus_tag="VYS74_000040"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV3788153.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            43242..44513
                     /locus_tag="VYS74_000041"
     CDS             43242..44513
                     /locus_tag="VYS74_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV3788154.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(44533..45348)
                     /locus_tag="VYS74_000042"
     CDS             complement(44533..45348)
                     /locus_tag="VYS74_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV3788155.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(45505..46158)
                     /gene="phoU"
                     /locus_tag="VYS74_000043"
     CDS             complement(45505..46158)
                     /gene="phoU"
                     /locus_tag="VYS74_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3788156.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(46170..46928)
                     /gene="pstB"
                     /locus_tag="VYS74_000044"
     CDS             complement(46170..46928)
                     /gene="pstB"
                     /locus_tag="VYS74_000044"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3788157.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(46941..47744)
                     /gene="pstB"
                     /locus_tag="VYS74_000045"
     CDS             complement(46941..47744)
                     /gene="pstB"
                     /locus_tag="VYS74_000045"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3788158.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(47755..48639)
                     /gene="pstA"
                     /locus_tag="VYS74_000046"
     CDS             complement(47755..48639)
                     /gene="pstA"
                     /locus_tag="VYS74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3788159.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGIYLHKKLTAA"
     gene            complement(48629..49546)
                     /gene="pstC"
                     /locus_tag="VYS74_000047"
     CDS             complement(48629..49546)
                     /gene="pstC"
                     /locus_tag="VYS74_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3788160.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(49546..50424)
                     /gene="pstS"
                     /locus_tag="VYS74_000048"
     CDS             complement(49546..50424)
                     /gene="pstS"
                     /locus_tag="VYS74_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV3788161.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(50998..52302)
                     /locus_tag="VYS74_000049"
     CDS             complement(50998..52302)
                     /locus_tag="VYS74_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV3788162.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(52303..53076)
                     /locus_tag="VYS74_000050"
     CDS             complement(52303..53076)
                     /locus_tag="VYS74_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV3788163.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(53066..53344)
                     /locus_tag="VYS74_000051"
     CDS             complement(53066..53344)
                     /locus_tag="VYS74_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV3788164.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(53344..53745)
                     /locus_tag="VYS74_000052"
     CDS             complement(53344..53745)
                     /locus_tag="VYS74_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV3788165.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(53955..54572)
                     /locus_tag="VYS74_000053"
     CDS             complement(53955..54572)
                     /locus_tag="VYS74_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV3788166.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(54562..55335)
                     /locus_tag="VYS74_000054"
     CDS             complement(54562..55335)
                     /locus_tag="VYS74_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV3788167.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(55346..56083)
                     /locus_tag="VYS74_000055"
     CDS             complement(55346..56083)
                     /locus_tag="VYS74_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV3788168.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(56088..57218)
                     /locus_tag="VYS74_000056"
     CDS             complement(56088..57218)
                     /locus_tag="VYS74_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV3788169.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDILHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(57302..57691)
                     /locus_tag="VYS74_000057"
     CDS             complement(57302..57691)
                     /locus_tag="VYS74_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3788170.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(57707..58090)
                     /locus_tag="VYS74_000058"
     CDS             complement(57707..58090)
                     /locus_tag="VYS74_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV3788171.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(58091..58879)
                     /locus_tag="VYS74_000059"
     CDS             complement(58091..58879)
                     /locus_tag="VYS74_000059"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV3788172.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(58872..59807)
                     /locus_tag="VYS74_000060"
     CDS             complement(58872..59807)
                     /locus_tag="VYS74_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV3788173.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(59937..60113)
                     /locus_tag="VYS74_000061"
     CDS             complement(59937..60113)
                     /locus_tag="VYS74_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV3788174.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(60253..60960)
                     /locus_tag="VYS74_000062"
     CDS             complement(60253..60960)
                     /locus_tag="VYS74_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV3788175.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            61111..62139
                     /gene="queA"
                     /locus_tag="VYS74_000063"
     CDS             61111..62139
                     /gene="queA"
                     /locus_tag="VYS74_000063"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV3788176.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(62800..63189)
                     /locus_tag="VYS74_000064"
     CDS             complement(62800..63189)
                     /locus_tag="VYS74_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3788177.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(63243..63934)
                     /locus_tag="VYS74_000065"
                     /pseudo
     CDS             complement(63243..63934)
                     /locus_tag="VYS74_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            complement(64649..65200)
                     /locus_tag="VYS74_000066"
     CDS             complement(64649..65200)
                     /locus_tag="VYS74_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788178.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(65550..66374)
                     /gene="nadE"
                     /locus_tag="VYS74_000067"
     CDS             complement(65550..66374)
                     /gene="nadE"
                     /locus_tag="VYS74_000067"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV3788179.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(66371..67831)
                     /locus_tag="VYS74_000068"
     CDS             complement(66371..67831)
                     /locus_tag="VYS74_000068"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV3788180.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(67947..68435)
                     /locus_tag="VYS74_000069"
     CDS             complement(67947..68435)
                     /locus_tag="VYS74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788181.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(68425..68634)
                     /locus_tag="VYS74_000070"
     CDS             complement(68425..68634)
                     /locus_tag="VYS74_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3788182.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(69020..69499)
                     /locus_tag="VYS74_000071"
     CDS             complement(69020..69499)
                     /locus_tag="VYS74_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788183.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
     gene            complement(69515..70307)
                     /locus_tag="VYS74_000072"
                     /pseudo
     CDS             complement(69515..70307)
                     /locus_tag="VYS74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(70743..71471)
                     /locus_tag="VYS74_000073"
                     /pseudo
     CDS             complement(70743..71471)
                     /locus_tag="VYS74_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(71731..72042)
                     /locus_tag="VYS74_000074"
     CDS             complement(71731..72042)
                     /locus_tag="VYS74_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788184.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(72057..73343)
                     /locus_tag="VYS74_000075"
     CDS             complement(72057..73343)
                     /locus_tag="VYS74_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3788185.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            73967..74149
                     /locus_tag="VYS74_000076"
     CDS             73967..74149
                     /locus_tag="VYS74_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV3788186.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            74159..74524
                     /locus_tag="VYS74_000077"
     CDS             74159..74524
                     /locus_tag="VYS74_000077"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV3788187.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            74521..75699
                     /locus_tag="VYS74_000078"
     CDS             74521..75699
                     /locus_tag="VYS74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV3788188.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     DLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     "
     gene            complement(76225..77217)
                     /locus_tag="VYS74_000079"
     CDS             complement(76225..77217)
                     /locus_tag="VYS74_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3788189.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            77377..79131
                     /locus_tag="VYS74_000080"
     CDS             77377..79131
                     /locus_tag="VYS74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788190.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            79115..80836
                     /locus_tag="VYS74_000081"
     CDS             79115..80836
                     /locus_tag="VYS74_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788191.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            80847..81431
                     /locus_tag="VYS74_000082"
     CDS             80847..81431
                     /locus_tag="VYS74_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3788192.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            81431..82126
                     /locus_tag="VYS74_000083"
     CDS             81431..82126
                     /locus_tag="VYS74_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3788193.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            82114..83478
                     /locus_tag="VYS74_000084"
     CDS             82114..83478
                     /locus_tag="VYS74_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788194.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
     gene            complement(84160..84724)
                     /locus_tag="VYS74_000085"
                     /pseudo
     CDS             complement(84160..84724)
                     /locus_tag="VYS74_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(84800..85012)
                     /locus_tag="VYS74_000086"
     CDS             complement(84800..85012)
                     /locus_tag="VYS74_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788195.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(85021..85326)
                     /locus_tag="VYS74_000087"
     CDS             complement(85021..85326)
                     /locus_tag="VYS74_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV3788196.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(85313..85507)
                     /locus_tag="VYS74_000088"
     CDS             complement(85313..85507)
                     /locus_tag="VYS74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788197.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(85677..86027)
                     /gene="tnpB"
                     /locus_tag="VYS74_000089"
     CDS             complement(85677..86027)
                     /gene="tnpB"
                     /locus_tag="VYS74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3788198.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(86008..86193)
                     /locus_tag="VYS74_000090"
     CDS             complement(86008..86193)
                     /locus_tag="VYS74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788199.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(86478..>86786)
                     /locus_tag="VYS74_000091"
     CDS             complement(86478..>86786)
                     /locus_tag="VYS74_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV3788200.1"
                     /translation="VRESDAILREEIAKAGLDRDIWQYFTVNTGVRSVGVMGDGRTYD
                     YTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYDITSKPPATVEWE
                     "
BASE COUNT        26619 a        18504 c        15842 g        25821 t
ORIGIN      
        1 acaatttaaa tgaattaaca attttcccaa ctaaaagcac tccagttacc gcaacggttt
       61 gtactgaatg tactaaatcg cattccatca acttcatctg tttcgtcaac ttgaacagat
      121 actaattgaa gatttaatac ttcttctgcc atagctaact cctcctattt aaatttttgg
      181 gattaagtgc tttatccacc ttcattatac tctctccagc agtaaaatgc aagcaatgat
      241 acaatgttgt cacgtagaaa ataatgtttc cgtaactttt caaagtaact tccatctctc
      301 tcccaaaact ggaagttagt tttagaagtt acctaaaaat caggttacct attttaaaaa
      361 agcagcaaac tataaactag taggctccac accaaatgta gccccatact gccccataag
      421 tccaatttgt agcgtacaag ccctaaaaac atccccagcg atacatacaa acaccaagct
      481 ataatggttc ctggatgatg tgctaaggca aataaaacac ttgtcaaagc aactcgaata
      541 tctaattttc taaccaagtt ccataaaatt tctctataca gaaattcttc aaccatactc
      601 gcattgatta aaaacaataa aaatgaaaac caaggaactt gatgttgaag tccaattaag
      661 tttgcttgat tcgtgctttc ttgcgcatgg attaggctaa aacatagact tataatcagt
      721 aggctaacga atccaatacc aagccatttc atcctagatt tcatatttac cttatgagct
      781 tgtttgcgtt ggccatacat ccataaaaaa gaaatgagtg acgaaccata gagaatctgt
      841 agtatagtta actcaccgat acaaagaaat ttcaataagt atagagatac caataggaca
      901 tttacttgtt ggaatatata aactggaatt attcttttca tagttacctc cgaaacacaa
      961 aaaaaggggt agacaatcta gtgtctaccc ccgaaagttt attaaaacaa aaatcctgcc
     1021 aaagaatttt tggcaggaaa ccaaatcaat ttatcagttt ctatcaatcg cttatcgctc
     1081 tcaaagactg gtaaataggg attccgcaat caaattgcga tactctatta tttaagagta
     1141 actgaagctc cagcttcttc caatttagct ttgatttctt cagcttctgc agttgcaacg
     1201 ccttctttaa caagtgctgg tgcaccgtca acaagttctt tagcttcttt aagaccaaga
     1261 ccagtgattt cacgtacaac tttgataaca ccaacttttt tgtcgcctgc agatgtcaat
     1321 tcaacgtcga atgaatcttt agcagcacca gcatcagcag catcagctgc agcaacagct
     1381 acaggagcag ctgcagttac accaaattct tcttcgatag cttttacaag gtcgttcaat
     1441 tcaaggattg aagcttcttt aatttcagca ataatgtttt caatgttcaa tgccattgtt
     1501 atttcctcca aataagtttt aaattttata atagtttttt tcgtagctag gctacgctat
     1561 gtagcttaag attaagccgc gtcttctttg ctttctgcaa ccgctttgac tgcaagagca
     1621 acgttgcgca ctggcgcttg aagtacagaa aggagcatag aaagaagtcc ttcgcggttt
     1681 ggaagagttg caagtgcaag aatctcttct ttagatgcga cagcgccttc gattgcacca
     1741 cctttaattt caagtgcttc agcgttttta gaaaagtcgt tcaagatttt cgctggtgcg
     1801 ataacatctt cattagaaaa tgctactgca gatggtccaa caaatacaga tgcaagatct
     1861 tcaagaccag ctttttcagc tgcacgacgc aagattgagt ttttaataac tttatactca
     1921 acttcgcttc cacgaagctc acgacgaaga actgtatctt gctcaactgt caaaccacga
     1981 gcgtctacaa cgacgataga tgcagcagct ttcatttttt cagctactac gtcaactagt
     2041 tccgcttttt tagcaataat tgcttcactc attagtgtgt tcacctccgt aattattttg
     2101 cttggggaat ttttcaaaaa gaaaaacgcg cccaaaccta gacacgaaag tacaatacgc
     2161 ttctttttac atgatacgtt ttgtcctcgg taggatattt atgagtcgag ctcccctact
     2221 gtcttaggca gtttttttaa accgtatata agtatagcat agtcaaaaaa agaatgcaag
     2281 atttttgcaa acttttttta aaatttttcg taatttttct tttaaagttc tactgtcagg
     2341 acttgacctt gcttaacaac ctgttctccg gcgatataaa catcatctac atcactagat
     2401 ttaactgcat aaaccaggtg agacagcata ttttcctgag gttggagatg aattttccct
     2461 tgtggttgaa tgaccagaaa atctgcttgc ttgccgactt ccagacttcc tatctgattt
     2521 tccattccaa ggaccttagc cccttcgatt gtcagtacct tgagagctgt ttcgattgga
     2581 aactggctgg catccccact tttcatcttc tgaagaagag ctgcagtcct tccttcctca
     2641 aacatatcta gattgttatt ggaagcaacc gagtcagtcg caattccgac tgctactccc
     2701 gctttttgga gctggataat tggagcaatt cctgatgcca gtttgaggtt actgatagga
     2761 ttgtgggcga tagccacttg agaagatgcc aagcgttcaa tttctctctc gtttaattcg
     2821 accccgtgag caaagacgga cggatgatct aaataaccca gttcttcaag aaaagcaagg
     2881 gggcgtttgc cgtatcgttt gaggataatt cctgactcct ccttggtctc cgccacatgg
     2941 acatggagcg gaatatttag ctcttttgcc atttccaaac tcgcttccag caagtctcta
     3001 ctgcagctat acggagaatg aggtgctacc ataaccttga aatttggatt tttatatttt
     3061 aagatttcgt ctatgatgga tcgagttctg cttatagtct cagcagttgt ttctgtctct
     3121 gaagaaaaga gagtcggaga aaaataacaa cgcatcttag aagttttcac cacctgataa
     3181 atttgctgga tatccacacc attgggatta tacatatcgt taaaggttgt tgttcctgac
     3241 tggagcatct ctgttagggc ttctttgacc gcattggtag tcatgtcggg agtaaactca
     3301 gattctgctg gccagatata gtcattgagc cattcatgga gattgctgtc atctcggatc
     3361 cctctcaggc ctgtcattgc agaatgggtg tgacaattga ccaaaccagg cataatccaa
     3421 gctccctgat agtctataat ctgctcagct tgctctaaaa acgctggctt atcttgaccg
     3481 acatagacga tttgagaatc cttgactgct aagattccat caagataaac atggaaatct
     3541 tgatcacaag tcacgatatt tacatgctga aagactttca ttataggctc cttttctaaa
     3601 agacaagttg tactctcttc aaaccacttc aaattcactt tgccgtaggt gtggttactg
     3661 acttcgtcag ttctatctgc aacctcaaaa cagttttttg agctgacttc gtcagttcta
     3721 tctgcaacct caaaacagtg ttttgagcaa cctacggcta gcttcctagt ttgctctttg
     3781 attttcattg agtattacag taaacaattt ttctagctat tgtaacaaaa aagtcactcg
     3841 aaggcaactt ttttcgtcca taaactttca aaagagaatg gcttattcag aactaaaagc
     3901 cgcagctttt tgcatttggt aatacttacc ctttctatcc atgagttctt gatggttacc
     3961 atattcaaca atatcaccat ctactaagac aagaattaaa tccgcatcct gaatggttga
     4021 caaacggtga gcaatgatga aacttgtgcg gcccttcatg agttttgcaa aggcatcctg
     4081 taccagcact tctgtccgtg tatcaatgga agaagttgcc tcgtctaaga taagaatctt
     4141 tggaatagcc agaaagactc gggctatggt caagagctga gcttggccga cagagagaga
     4201 ttctccagca ttttccaact tggtatcgta tccctgtggc aactgttgga tgaaaaagtc
     4261 tgcattagct gctttggcag cagcaattac ttgctctcga ctggcttcag gattgccaaa
     4321 ggcaatatta tcatgaatgg tcccttgtgt gagccaggtt tcttgaagca ccatgccaaa
     4381 ctgctgtctc aatgatgctc gtgtataatc ataaatggat tgcccatcca gcaagatatc
     4441 tcccgagcta atgggataaa aacgcataag gagattgata agagttgatt ttccagcacc
     4501 tgtcggacca acgatggcta tcttactacc agctggaata tcgatagaca agtccttaat
     4561 caaaattttt tcaggatggt agccaaaaga gacatgttta aaggaaatag ctcccttaac
     4621 ttggtcactg gtcaagactt ccttacctgt ttcagccact tcagtgctat ctaagactcc
     4681 atagatacgc tctacgcaag ccagagcact ttgcaactca gctagcactg aagaaatatc
     4741 attaaagggc ttggtgtatt gctgaacata gttcaaaaaa gtcactaaac gaccgacggt
     4801 caaggttgaa cccatcatga tacgataagc tcctactcca gctaaaaggg cataaatgag
     4861 tgcatttaca aagcgagtcg aaggattgac cgttgaagaa taaaagatgg ctgactgaga
     4921 atagcctgag tagttgtcat gagcctcacg caatctttgg ataaattctg tttgagcatt
     4981 gaaggactgg attatagtct gctggctaag cgattcttca atcaactgag tctgaattcc
     5041 cctcgtctct gtttgcttct ggaagagatg ataggatctc ttggcaataa agcgtgaaat
     5101 caccatggac agtggcgtca acagcaagac taagagagtc atgaggagat gaatttggag
     5161 catggctaga atactaacca aaatcatcaa aacaccaatg aaaaattggt taaaaatcat
     5221 ggtcaagcca gctgccaact gttcgatgtc cgtggttaca cgactgacca tctctccatt
     5281 gccttgccga tccgcaaaag caatcggtaa ccgatggagt ttatggatga ttcgctctcg
     5341 taaatctctg gtataagaga agattagacg attatagagg agaggattgg cccattgtac
     5401 tagagtattt cctattacca ccaagagcat ctggagaaaa atctgccaaa aaactggtga
     5461 tgaaccagcc actaggactt ggtcaatgac ctgcccaatc agaataggta gataaattga
     5521 taagccaact tgggcaatag ttcctagaaa ggctaggaaa aggaggaagg gatggtttgc
     5581 taaatctacg gctaaacgtt tgagcgtctg gtttacagtt tgtcgtttca ttctagtcct
     5641 cctttccatg ttgggatgca ttgatttcac gatagacttg actggatttc atcaagtcat
     5701 cgtgcttgcc aacagctagc aactcacctt tttccaagag gagaatctgg tcagccatct
     5761 gtaaagtcga ggttcgttga gagatcaaaa ttaagctcat gtttggaaaa ttttctctaa
     5821 tagctttcaa gagcttggac tcggtaatgg tgtccagtgc tgaggtcgca tcatctagga
     5881 tgagaaacgg agcctggcgc aagactgctc gtgcgataga cagcctttgt ttttgtccac
     5941 ctgagaaatt tcgccctcct gcttcaacta gagcatccaa gagtccttcc ttatcactga
     6001 caaaatcctt agcttgagca atttccaagg cttgccagag ttcttgatca gtcacttctt
     6061 gatttaaacc taaagtcaag ttggaacgaa tagttccctt aaaaagttcg accttttggg
     6121 gaacataagc aatccaagac cgccactgct ctaaattacg aggactagat ccatctcgat
     6181 aaaggtcaat gctcccctta tctattggat aaagtccaag taagagttgc accaagcttg
     6241 acttaccaga acccgttccc ccgatgatac ctagaatttg tccttgagtc atatcaaagg
     6301 aaatgtatct cagagaaggc tgggccgcat caggataggt aaaagtcaat tcttggactt
     6361 gtaaaacccg accactggta acttgctttt gttctaattc tgaatggatg ttttcgggag
     6421 attccgcaaa gacctcctcg attcgcttgg ctgagatata ggactggttg agagaattta
     6481 tcaacatggc tagcttgacc aattccacca aaatctgtaa gaggtaattg ataagagcaa
     6541 tgagagcacc ttgactgagc actcctcctt gaattgaaat atagccttgc cagataataa
     6601 cgagaagagt tccattgaca atcagatagg tcagaggtgt taataaacta gaccagaaac
     6661 ctgtcttttc ttgtaatcta gcataaactt ggttaagggt ttgaaaaatc tgtaactctc
     6721 gtttttcttg accaaaagca cgaataaccc gcatcccttg caattgctgg cgcgtttcct
     6781 gaaccagttg gtccgttttc tttctgagac tactgtagag aggattgacc aatcgagata
     6841 accctacaat gacaatggtc aaaatggcaa ccatgactaa gaaccagaaa gtcaactcag
     6901 ctgagattcg ataagccata aaaatggcac caaaaacgat aatgggcgct cgtaaaaaga
     6961 gacgcaggaa ttgattgata ccagtctgaa tctggtaggt atccgaagtc aagcgagtaa
     7021 ccaaactaga agttgtcaga cggtctctgc tgtcctttgg caaggaaaga atatgacgat
     7081 aaagatcgtt tgtcaattcc ttagcaaaac ctactgctgc ctttgctgag taaaattgag
     7141 ctatcaaggc cactaaaacg ccaattactg caaagataag gagcaggcca atctgcatcc
     7201 agagatgacc ttgatctccc tgaggtaaag attggtcaac aatcccagca atcaccatgg
     7261 gaaccaagag ctcaaaaaca gcttctaaca gcttgaacaa gggggctaaa attgattcct
     7321 tgatgtaggg tttgaagtaa gataataggt gtttcataaa tcccttctat tcatattcta
     7381 gaaatgaaga aagtgggaag ccccactctc tgttttattt gtttaagtaa ggcaatagat
     7441 agccatatcc tgctttttcc atctcatcct tggccacaaa gcgtaaagaa gcagaattga
     7501 tacagtaacg gaggccgcct aactcccgcg gtccatctgt gaaaacatga cccaagtgag
     7561 cactgcctga acgagaacga acttcaattc gctccattcc atggctcaga tccttgtaat
     7621 aatgaatcaa ctctttggaa atcggacggc taaaacttgg ccaaccacaa cctgaagcaa
     7681 acttatcctt ggcaaaaaag agtggctcac ctgtcgtaat atctacataa atcccctctt
     7741 caaaggtttg gtcataggca ttggtaaatg gagcctctgt agcagcttct tgtgtgacac
     7801 gataagactc ttcagataga ctggccttca acacctcttg actaggcttt tcatagtttg
     7861 ctgcatcaat caatggctta tcagcatcgg tcacatcgat atgacagtaa cctgaaggat
     7921 tcttcctgag atagtcttgg tggtagtctt cagccagaat gtagtggcgt aattgctcca
     7981 cttctactgc aatctttcga cccagcatgc gttcctgctc ctgcaccact gtgtagatag
     8041 ctggcaaatc tgcttcatcc tgataataaa tcccagttcg atattggcgg ccacggtcat
     8101 tcccttgttg attgatagat agaggatcga taactcggaa ataataaagt aaaatctctc
     8161 tgagtgacac ttccttctca tcgtaaatca cttggaccgt ttctgcatgg tctgtttcct
     8221 tgatcaactg gtaattggtc gtttcgactt gaccattagc gtagccaaca ctggtttcta
     8281 gcactccaga aatgcgtgaa aaatattcct ctaggcccca aaaacaacca cctgctagat
     8341 aaatttctgc catctttatt tctcctttat cacgttttta actaatactc ttcgaaaatc
     8401 tcttcaaacc acgttagcgt caccttaccg tatatatgtt actgacttcg tcagttctat
     8461 ctgcaacctc aaaacagtgt cttgagctga cttcgtcagt tctatccaca acctcaaaac
     8521 actgttttga gcagcctgcg gctagcttcc tagtttgctc tttgattttc attgagtata
     8581 aacttctttt cacaaaaagg atatgaaacc ctctggtcaa gagtttcccc atcctatctt
     8641 ctattcttta ttttgcttct acacggacac cttgggtatc aactctcaag tcatgcagtt
     8701 ttcctttgaa aggttgcttt tccaattctg ccttaattgt tggcatcttg tcatgagaag
     8761 ccagaaccat aactgtcggc ccagcaccag aaaggtaggt tgcataggcc ccattttctt
     8821 tggtcacttg cttaatcatc gcaaattctc ttaccaagtc ctgacgatag cgctcatgga
     8881 agaggtctcc ctcgattgct tgcccagcgg tcaccatgtc tcctgccaac aaggcagcaa
     8941 ccgctacatt ggcgatagaa cttgcagcaa cagcttcctt ataagacaat tttttaggca
     9001 agacactacg gctgtcgcga gtacgtaatt catagtttgg aatgtaagct agaaaatcac
     9061 actctggaaa gtctgctacg atagcagaga cttgcccttc aacagaactt gcaataacga
     9121 gattaccata aatggctgga gccacattgt caggatgccc ttcaatcttg gtcgctaact
     9181 gcaatttttc atggtctgat aagttgagtt gacccagttg gttggctagt tcaatcccag
     9241 caacgataac cgagctggaa gaacccaaac cgcgcgccaa agggacatca ctggtcattt
     9301 tcaagcgtct tggttgcaag tctggtacaa tttgcaaagc gattttgagc aagagattac
     9361 gctcatcatg tggaatccat ttgccaatct ggtgttcaat cagccactca tctcgttctt
     9421 cgcagacctc aatttgaaga tacttggtta cagctacacc gaccgagtca aaacctggcc
     9481 cgatattggc actggttgca ggtacaataa tcttcatctt attctcctag caccttgaag
     9541 gtattcaaga ggtcgaattc tgaaaccttc ttcaattcag ctgagacatt ttcaagctgg
     9601 gctttattaa tcttgtgtgt gatgataacg acacgcgcct tgtcaccctc tttgccatct
     9661 tgaaggattt gcttaaagga aatatcttga gcattgaaga tttcagccaa cttcaagacc
     9721 tgaccttttg agtctagagc caagattgag aaatagtagt ttgctttgac atcttcagga
     9781 tttgccaaga ccaagtcacg gctatattcg ttgaagtctt tgccaatagt accatcattc
     9841 aaacgacgaa cgatacggac aatatcagct acaacacttg ttgcagttgg tttttgaccc
     9901 gcacctggtc cgtagtacat agactcacca ataccgatag attctacaaa gacagcgttc
     9961 attacgccat tcacactagc aagtgggtgc gctttaggta ggaaggttgg agtcacttct
    10021 gcagcaatac ctgaagaagt ttcctcaata gaaccaacca atttcactac gtaaccaagc
    10081 tcttgagcta cagctacgtc ttctggtgtg atattgcgga ttcccttgtg ggctacatca
    10141 tcaaaggcaa tcttcatgcc aaaggcaaat tgactcaaaa taaccatctt gtaggctgca
    10201 tcaatcccat ctacgtcatt cgtcggatcg ctttctgtaa atcctagacg ttgtgcttcc
    10261 gcaagagcat catcgtaaga ccagccttct tccaccatct tggtcaccat gaagttggaa
    10321 gttccgttga ctactccaag cacgcgcgta attttatcag aagccaagga atttgctaaa
    10381 gtacgaagaa ttggaatccc accagcaact gctgcttcgt agtaaagtgc taccttgtta
    10441 gcttgagcga tttctagcaa ttctgcgcca tggacagcta aaaggtcctt gttagcagta
    10501 acaacgtgtt ttccagcttc caaggcacga gtgataaagg ttttagcagg ctcaatacgc
    10561 cccatcaatt ccactacgat agtaatatca tggtctgata aaatatcatc cacattggtt
    10621 acaaagttaa agtcattccc tgctgcaagc aagcgatttt tttcatcttc atccttgacc
    10681 aataccttag caactttgat atctgaatgt gctgattgat tgatttttcc tccattttcc
    10741 tttaggagga aaggcacacc acttgcaacg gtaccaaatc ctagtaaagc aattttaact
    10801 gtcatcttta tctcctcgaa attttctaat atagccatta taacagaatt ttgtgaaaat
    10861 tcctattata gtaaatcact atttcagtat aaaaagaaaa aacgaatcag acgattcgct
    10921 cttcttaaaa tctgaaaata gctttccaga aaggattagc cgattttttg cagattgagc
    10981 actgcatcgt gactcatcaa gacttgacca tactcttgta agactgagcg actgatatca
    11041 ctatcgtctg caaactcgcg catacgggcc aacagccaag ctggatatgg gcttggatga
    11101 ttttcaatat ccactaaaat ggtcaaataa tagcgctcgt tcattttgta gagttcagaa
    11161 gtttccattt caaaagtcac tgtcttggca aaaactacca agtcagccaa cttagcaaaa
    11221 gaaaggatgt agtagatgta aggttctttc ttactctcag cttcttgttc agcctgctct
    11281 tgctcttctt ccttggcttc aacttgctca agagattgaa tggcttcgat atcatccttg
    11341 gttttgtctg cgatgctttt ttccagggtt ttgataaatt catctggaga catttgagcc
    11401 aattcttcca tatctggcaa atccgataag tcttcaaaat ctagattttg gtcaatcttt
    11461 gacttggtca caaagacatc taccttatca ggttttggag tcacacggaa gctcaacatg
    11521 cctgtatcca gaaagctatc aggcatctct agctcatcca agatagcata aaagaactct
    11581 tctgtttttt cttgaggaac gagaaagtca gcaatctcca ttccacgatc catcaaatcc
    11641 tctaaagaca tcgtgatttt taaagttgta tcactaattt gtttcatttt cattgctagt
    11701 aacctcatac tttcagttct atctattata ctagattttt acgattttat caaaagaagg
    11761 ctcctctata cggatagatt ttccctaggg tctttctata ggagactcca aaagaaaatt
    11821 tctgcagaca gatagaaaaa gccttcaaaa tcagctctta gccgattttg aagaccttat
    11881 acatcagaat acttataatt taaaggttgc tacaccaagg atagaacgat ttaagtttct
    11941 gagaatttga agactttgct caaatttctt ataacgagtc actccgtact cttcaacaag
    12001 aaggactgta tctctttcca aaagagatga tacatcctgt aaatctacaa aatgcattcc
    12061 ttttaaagct tcttgactct gtttcaattt atctaggata gctttatttg agctaacgat
    12121 ggtcaattcc tgtccagtat ttttgtatga caaaacatct gctaggttag caattgttgt
    12181 aatctctgtt acaaaatcaa tttgatactg agaaaaatca cctactctat tgattgttgg
    12241 attaaagaga taaactaaca catttcccat cacaaccaaa atcacacaaa ccactccaat
    12301 aacaactaaa cgaagaatca gatttttcac atttaagcca agcgctgttt caccatttgc
    12361 gttcaattct ttagagttga tggtttccag tttttcaatt ttcacatttg cataggcatg
    12421 tttaaatttc tcaatcaacc catcaatttt tttctctaac aagtcattgg catctttact
    12481 tgatgtcaaa attttcacac caacccctgc atcgtcaatc atatagtaga cggtcaattt
    12541 tttccaccaa tagtcattcg ttgaattttt caaggttgtt tctgtcgtgt ctaattcact
    12601 ggcaattttt ttcaactcac tgggttctac atcattgaaa agataagctc cattcaaatt
    12661 accatcaatc aatttcccat aaaaatcact ataaccacca atttgatgat tcaaaatcgt
    12721 tttggccgac tcttttggag gagtgatttt atagataaga taagttgaat aacttgttgt
    12781 atctttgaca gtgtttttat tcctaactgc tttaattgta aatggtacag caatgagagc
    12841 aaataaagcg atgagagcta aaatatttgc ttttcgcttt ttataaagat ttgcaaacaa
    12901 atcagctact gaataatgtt caaacatgat ttttttctcc tttgtttagt agatactagt
    12961 tttcctttgt aagcattttt gctacaaata taatcacaag aacaattccc cagaattgca
    13021 ttgtaaataa attgaagaaa ctttctgaaa agctgcttct tggcataaag aatagattat
    13081 tcaagatgag tagggataaa gcaaatagga ttgtccttga gcgataggct acttgcagca
    13141 tggctataaa taatacgccg agtaagaaac taagcagaaa gactccaatc ataccatagt
    13201 cggtatacaa ctccatgata taactacttc cgataccatg ccctttcaag tattccttgt
    13261 tcaagacaag ataggataga ttgtgggcat aactattact atcaatagct agttccacac
    13321 tattggttgt atgttcaaag gcttttcctc cgaaaatggc tcccaaactc ccccttgcaa
    13381 aataatcaag aacaggacca aaagtaaaat tacggaaatc tcggtaaggg aggctactgt
    13441 taaatagaaa acctcgagcc agaacaccaa aactagtccc ttgtttatag ataaagtcaa
    13501 gtaagatatc ccagaaacct gtatgagaaa cttggacatt atcccgtaca taattgagta
    13561 ctcccatcgc taacatgaga ataggagaac ctacaaaaat cgctaacttt tctttaaacc
    13621 caatccattt tcctttttca gtttgctccc gcataaagta ataaacaaaa gcaaataaaa
    13681 tacttaaaat aaagggattt cgtgtcccaa ttgccaaatg aatagtatta gctgcaataa
    13741 aggagacaag cactgctgtg gcctgcaatt tctttggctt ggttgccaga tacatacaca
    13801 ttgcatagac cgtaaaggta gacaaaatgt aggtaaaata aggcagttta ctttcaaaat
    13861 ttgcatagta ggcatagtag gaagtctgca aacgatacaa gagccgttca aataaccgaa
    13921 tgaaatagaa aggataggtt agaagaaaaa ctcctagtga tacaaagcgt aaccgcttga
    13981 tataaacctc ttttagagaa tttcctatat ttgctacttt tattttcttc ctagctatga
    14041 agtaacgagc cagaatgcct cctgtggtca agcccagaat cgaaaccatg acaactataa
    14101 aggcaaaacg ataggctatt ggatgatagg tatccaaagc accatcccta aaataatcaa
    14161 tggtcggtct tgataccaga aatacaaaaa tggttaaata gaaaataaaa tggattaagt
    14221 aatacttgat atcattccaa caagcaatta agctactaac caacaagaac aataaagtag
    14281 aaagtaagct aacattatta ttattaaaca gatacacaat tccacttact agcgtcaagg
    14341 cataactgac tatggtcaaa ctaaataata atcgtttccc atcaatcact tggtcacccc
    14401 cgttctaatg taatttttta gatttttcaa tatttttcag taataagaat cgatataagg
    14461 aaatatttat gaatagggcc aaagcactaa ttcttctccc cttacggaaa attggattcc
    14521 tagaaatagc aaaggcatgg ccttttaaaa aacgatgaat ctgagaatag gcttcaaact
    14581 gtttatactg atcatctagc aacatcttat ccagaataaa gaagtgggca taggccaatc
    14641 tgaaaaaagc gacctctttc aagtcaggat agtttttcac aacttcatta taaaactttt
    14701 ggtagatatc aatataggct aaatccttct ctgcataggg tttggtcgta atactatccc
    14761 ctctatggaa atagtaataa tagggtttag tattaaccac atacttcttg gccaacttga
    14821 ttaaatcaaa atggtaatag gcatcttcgt aaatcaaccc cttaggaaag gatagggcag
    14881 ttgcaatctg tctcttgatt agcttattgc aaatcgtccc aggtattttt tcacctatga
    14941 ggtattcctt tagaaatgtt tgagaatcac agacaaaata gtcatcctga ttggctgact
    15001 gtgggctttc atcattagca tagacattca tgacaccaca gcttgaaaca tccgcatctt
    15061 cttgaactaa ttgctcatat aagctctgaa tcatttctgg atggatataa tcatctgagt
    15121 caataaaaat cagataatcc ccgtgagcct gcttcatccc atcatttcgt gcttgcgaca
    15181 atccttcgtt ctttttatga agcactgaca ccctgtcatc ttgttcagcg attgaatcac
    15241 acaagcgacc actttcatct gttgcaccat catcaacaag aataatttcc agattttgat
    15301 aggtctgctt ctgaatggaa gctatcgatt tttctaggta ctgcgccaca ttatagactg
    15361 gcacaatcac actaattaat gcagtttcca tgctactcct ctaatagttt ttctacttgt
    15421 tcgatttgtt ttgtaattgt aaattgttga atgaattggc tagcctcatc gacatcaaag
    15481 tttgaggcag aagtcatgta attagtaatc gcctgagctg cctcttgatt gctctcaatg
    15541 atttgtccaa atcgtccttc ttgggataat tcctcagccc ctccaacgtt cgtagagata
    15601 aaagggagtc ccagactcaa ggcctccaca tacactccag gaaaaccttc ttgtttagac
    15661 atagacaaaa gaactttcgt ctgagataga tactgataag gatttttttg ataaccaagg
    15721 aaatgtacat agtcctcaat cccatactct ttgactcgtt ttttcagttc ctcttccata
    15781 tcaccagccc cgataaaata gagatgatag ttttttccct cttggtgtaa taatcgtatc
    15841 acttccacta cacggtcaga acccttattt tcctcaatcc gtccgatagt acagatactt
    15901 tgaggagcaa tctcgatatc gatcttctct tgagattttt ctagaatagt ctgaaaatca
    15961 tatccattgt agattgtctg taatttagaa gtataatctg gataaacttc cttgatagaa
    16021 ttgctggtct tttttgaaat ccctacaatt gtattcgcag catccaactg gcttctatgt
    16081 gattctcttt tagagctatc cttaagaagt tcttcaatac ttccatgaat ccaagatatc
    16141 ttcttgactt ctcttctttt agagaacaac agtggtggat tcataatggt aaaagaaact
    16201 tcaacatcat aatcatcttt tacaagcaaa cgacgagcca gtcttggaaa ataaattctc
    16261 attctccaca aaaaagctcg taaccatctg gtttggcgat aatcttgaag ggattttaaa
    16321 atgcgtacat gctttggaac agattcatat cccttgtcaa agtgctccat ttcaagaata
    16381 tcaatatcat acttttctgg atccagattt gaaacaatgg ttgatagaat cttctctgca
    16441 ccacctccaa gagaaaaaga ccacataaaa aataagattt ttttcttagc caccatattc
    16501 tcccttgtat tctgtataag acttatccat atcagcgatg acagcatcat gatgcggtac
    16561 ctgcttgtct gctggtggag gcgtcatata atccccaaaa gcagttctga gatagacatc
    16621 atagccgatt ggaataggca tctctgttcc ttcaaatggc aagaaaagat tgtcttcaaa
    16681 agatgtgatt gggtacttgt ttctcatgta gccaggacct gagcataatt ctgtaatgcc
    16741 atcacaatca gccaaatcat acttagtcat ttctttctca gcttttttcc agatgcgata
    16801 acagagagat tttggagtca aacccagtaa aatgcgactt ccccatttca tgagagcacc
    16861 atgcttttct ggaatagttt gcgcacaaaa gagtgaataa atcaaggccc aacgaacctg
    16921 ttttttccgc tcagctggat ttttcggata ataatccaaa ggcaaaacat ccaaggccag
    16981 accatgtggc aaatccaaat cctgctgata aggcttgata caggtggttt ccttgtcacg
    17041 aatggtaata aaaagattac gatcaacaaa atccttgtga ctctttgaca agaaataacg
    17101 ttcatctgca taacgaggcc ataattctgc taatttctca taatctttac gaggcataaa
    17161 aaagtctagg tcgtcgtccc aaggaataaa tcccttgttt cgaagggcac caatagcgcc
    17221 tccgccacag agataacaga gcaaatcatg ttctttacaa aaggccacaa aatattcagc
    17281 catctccaga ctacgagcct gaattgcttt taaatcagtc atattgttca ttattctttc
    17341 tatcgtatcg tttcattata ccacaaacaa ggggtgaaaa tctattgcag actgtaaaaa
    17401 atcaaagcct gactgctatc caaatagcta tcaaactttg atttttctgt cttatactct
    17461 tcgaaaatct cttcaaacca cgtcagcttc accttgccgt aggtataggt aactgacttc
    17521 gtcagtctta tctacaacct caaaactgtg tttttagcag cctgcggcta gcttcctagt
    17581 ttgcactttg attttcattg agtattatct tatctcaagc ccatttgagc gagcttggtt
    17641 tgatatttgt tttgatcaac cagcaagccc aagcctccat aaacatcata ggcatctacc
    17701 cagtcaccca gttctggaat cgtcaatttt tcaataccat tttttgctcc atccaaaaca
    17761 gataaaccgt ttgttaggag gaaagtatag ggtacgttgg ttgaggtcat agcaaaaacc
    17821 tttccaagag cttcagaacc agtgaaaagt ttagtgggat ctttaatttg ctctaaaatt
    17881 gctgttaaaa cttgttgctg tctttttgta cggccgtaat ccgcctcatc atcatcacgg
    17941 aaacgagcat aattgagcag ggtcgagcca ttcatctgct gttttccgac tttaatggtt
    18001 tgggttggag actcagtctc ggtagcgtat aaatcatctc cgactgtagc ttctgttagg
    18061 ggacgcccat tcaatgttga aaattgagca tcaatcgtca ccccatcagg gaaaagcgtg
    18121 tcaatcgctg tggcaaaggc ctggaaatca accaaggcgt agtacttaat gtccaagtca
    18181 aaattatctt tcaagacttg gcgaaccatt tctgcccctt tttgcccctc ttgttctcct
    18241 aactcgtagg ctacgtttaa cttgttatct gtctgttttc taccgttaat cacttgacta
    18301 taaccatcta tatagaccaa attatcacgc atgaaactga ctagcttcat tttcttatct
    18361 gagcccccga catttaatac cataatagag tcagttcgtg tctcaacact gttctggccg
    18421 attcgaccat cagtacccat gattaaaata ttaactccat ctctagtgtc ctgaccatta
    18481 aagacttcta cttgagctgc ccgggcatca gcagttttct ttgcgctagc atcttggtaa
    18541 ccacgcaaaa acatgaatac catggccaaa gccacacaga ccaaaagtga aaaaatcacc
    18601 ataaaaattc gtttaagacg gagcttccgt cttttctttt ttggagggga agagagtgct
    18661 tgtgatttgg attgtgagcg actccggttc gcatagcttg gtaagtcaac ctgctcttct
    18721 ctttcttgtt ccaagctaga gctactattt cccctagcaa gagttagctt ttcttgcaaa
    18781 taggcaaact catttttttc tctctcattg agatagtgaa tattttttag caaataatca
    18841 taacgcaact gctcatgatg acttaaggga ttttctttac tcatcttctc tcctttccat
    18901 ggtctgatat tggataaata ggataggcac ccagaatttt atactggatt ccaatcgctt
    18961 ctaattcttt ttgggcaaag tggaccaagt ccttatcggt ataatccaca tcgataatga
    19021 aaaagtattc acccagtgct gtcttgagtg gacgactttc aatttttgtc aagtcaattc
    19081 ctcgccaagc aaaggtcgac agggccttat aaagtgcacc tggaaggttg tcaggtaatg
    19141 tcaaggccaa actcatcttt tcagtttgtg cttgcaaggg aatactaggc ttttcagctc
    19201 ctagaaccca gaaacgtgtg aaattggctt ccatttcctg aatatcctcg gcaatcagtt
    19261 ccaatccata ttcttcagca gaacttctag gtgcaactgc tgcaaagggc tgatctggat
    19321 gttcggaaat aaaacgggcc gcataagctg tactagctgt tacctcgatt tgagcctctg
    19381 gatattgttc atcgatgaat ttctttcctt gagccaaggc ctgtggatgt gaaaaaatct
    19441 tttcaatctt agtatggcct ggaaccacca tcaactgctg atgaataggc tgaacgattt
    19501 ctgctactgc ttggatgtga gcctgatgaa aaagatagtc caaggtttca tgaatactac
    19561 cctcaataga attttcaact ggcaccacag aatagtccac taatccttgc tcataagcct
    19621 tgatgacatc tgtaatgttg gcaaaagcct gcaattcctc atgaggaaaa gctgtctgca
    19681 caacgtggtg tgaaaatgat cccttgggac ctagataagc aattttcatc ttagttcctc
    19741 tataatttcc tctgggctta gcttagtcac atccaaaacc cgactagcca cttcctcata
    19801 ccaagcctgt ctttcttgaa aaatagctac tagttcttcc ttgctattat ttagaaaaag
    19861 cggtcgctga ttgtccttat cagctgcgat acgttggtag agggtttcaa aatctgcttt
    19921 caggtagatg ttatctgtat tagtcttgag taaatcacga tttctctgag aaataaccac
    19981 tcctcctcca gttgacacga cttggtctgt ttgtagtaaa tcagctagga cttctgattc
    20041 tacctgacga aaggctgctt ctcccttttc agcgaaaaaa ttcgcaatgg acatacctaa
    20101 gcgcttctca atcagagcat ccatatcaag gtaattaggg tccaagcctc ttgcaatagt
    20161 cgattttcca gcccccataa accctaataa caccttagcc atgaatcaag ctctccaaat
    20221 catcaaagaa actaggatag ctggtattga tggcttctgc acggtcaagc tccacctctc
    20281 cacctgcaac caatagggct gcgatagctg tcatcatgcc gatacggtgg tcaccaaacg
    20341 tattgactct agcaccgtga agagctgatt ttcctttgat aatcatccca tctgccgtag
    20401 gagtaatatc tgctcccata ctatttaagg cgtctgccac aacctgaata cggtctgttt
    20461 ccttgacctt gagctcctca gcatccttga taactgttac atcttgggct tgggtcgcaa
    20521 gtagggcaat aataggcaat tcatcaatca aacgtggaat caaagcgcca caaatctctg
    20581 ttcctttcaa gtcagaagac tcaacaatca aggttgcaga tttagcgact ggatcgattt
    20641 cagttatttc caattttcca cccatggcac gaatgacatc aataataccg gtgcgagttt
    20701 cattgatccc cacattctgc agcactagac gagaatttgg aacaatcaaa cctgcgacta
    20761 accaaaaggc tgcactggaa atatctcctg gtacgaccac cttctgtcct gtcaattttt
    20821 gtggcccttg gactgtgatt ttcttaccgt ccacacttaa atgaccacca aattgtttca
    20881 acatatcttc agtatgatta cgggtgcact ctttttcgat aataactgac tcccccttag
    20941 cttgtaaggc tgcaaacatc aaggctgact tgacttgggc agaggcaatt ggcaactcat
    21001 aatgaatagg tcttaggttt ttcgtccctt ttaagcgaag gggaggcaag tctcgttcag
    21061 tttgccctga aatgctgacg cccatttttt tcagtggaag ggtcacacgg tccataggac
    21121 gtttggaaag actatcatct ccaaacatct ctacttcgaa atctgcacca gcaaggacac
    21181 ctgaaatcag gcgaatcgag gtgccagaat ttcccatatt aagggcattt tgtggcgctt
    21241 ttaagccagc catgcctaca ccttgaatgg taataacccc atctttatcc tcaatttcaa
    21301 caccaaggtc acgaaaaacc tgcatggtcg aaagaacgtc ttcacctcgc agaatatcat
    21361 aaaccttggt ctcaccctca gccaaacttc caaagataat ggaacggtgg ctgatagact
    21421 tgtcacctgg gacgcggata ctaccatgta aatggcgaat gtttgttttt agtttcatac
    21481 tggacctcat acttgcaata cttttaccta ttttatcata aaaagtcaga aattccttaa
    21541 aaattcctga ctttaggatc gttcttttct tatttcagca attctgaaac tggttcaaaa
    21601 acaatttttt caatgtcaga aaggtaaatg gccaattgtt gttgcttggt aaagaattct
    21661 gacaagaggc tatttccttg aatctgttta ccaaagcctt ccatcttagc ttggaaggac
    21721 gcatctggca tttgacctgt ctgtgctagt ttttgaattt cctcttgaaa ggcaagatat
    21781 tctgtaaaga ttttgcttgc ctcagcatct gctgcaatcg catctttagc tgctttaaca
    21841 gccttgtatt ctggtaatcc gcgtagaccg cgactgagtt cgtttgcact atcgtaaata
    21901 tttgacatgt tcttctcctt atttgatgac gactgtatag tcggtatttt ctgttatgag
    21961 atgctcagct ctttccaagt cttgagcatt tttaaatgaa atttgtagga ttccgtgaat
    22021 atcctcacga ttttcctcgt tgatgtggat gttaaccaag gaagttccac gtagcagttc
    22081 caaaatccgc aggatgacat cttcttcatc aggaacgtca acatagaggt cgtaagagct
    22141 atccacacca ccacgcttat ggatttccat ggtctggcgt tgttcacgcg cttggttaaa
    22201 aaagttccaa atttgctctt catctccctt actaatggcc tgaccaatca cttccaaacg
    22261 ttccttgaaa tcctcaattc tatccagaat ggtctcgcta ttggacaaga gaatggaggt
    22321 ccacattcct ggctcgcttt ccgcaattcg ggtcatatct cgaaaaccac ctgccgcaaa
    22381 gcgccttgcc atctcatgct cttgagcata gaccgcagtc tgctccatga gactagaagc
    22441 caaaatatga ggaaaatggc taatctgaga agtgacacga tcatgctcct tggcatcaat
    22501 ctcgataaaa cgagcatgaa gacctgaaag cagatccttc atttccttaa gcgtgtcctg
    22561 acttgtcagg cttgaaggtg taaagatata ataggcattt tcaaaaagat tgacatctgc
    22621 cgaagcagcc cctgtcttgt gactaccagc catgggatgg gccccgacaa agcgaacaga
    22681 cttgccagcc aaatactgct ccgccgcatc cacaatggtt gacttggtcg aaccagcatc
    22741 tgaaataata acgccttctc gcaaatccaa attggccaac tccttaatga aagcaatagt
    22801 ttgtttgatt ggcaagctga ggataatgac atctgccaaa ggagcaaaac tagcaaaatc
    22861 atccgttgca cggtcaatca taccttcttt caaggcgata tctctcgaag cttgactacg
    22921 attataacct aaaatttcat aatctggatg atcgcgtttg ataccaagtg ccatagaggc
    22981 tccaatcaac ccaagacctg cgatatagat tgtttttgcc ataggaactc cttaatagtt
    23041 ctttgtatag tctcggtgtt tggctaccgc ttcttttagt tcctcaagat tatctgatga
    23101 gaatttttcg aggatttctt gcgccagaac cgttgctaca actgcttcca tgaccattcc
    23161 tgcagctgga agagcagtcg gatcacttct ctccacggtt gccttgtaag gttcgtgggt
    23221 ttcgatatcc acactcataa gaggtttata aagagtagga atgggtttca tgaccccacg
    23281 aacaacgatg ggttgcccat tagtcatacc accttcaaaa ccacctagat tattggtacg
    23341 gcgagtataa ccgtcttctt tagaccagag aatttcatcc ataacttggc tgcctttacg
    23401 ataaccagcc tcaaagccaa gaccaaattc caccccttta aaggcattga tagagacaac
    23461 agcttgagcc aatcttgcat ccaattttct atcccattgg acataggaac caagaccaac
    23521 tggaactcct ccgacgactg tctccacaac cccaccgatg gtatcaccat cacgtttgat
    23581 ttggtcaata tagtccttga tttcctgttc tcgttcttgg ttgacaatag aaacttcaga
    23641 ctgggcagct ctttgcttaa tttcagcgac tgtcagattt tcaggaacat ctatttcctt
    23701 accaccaaag accacgacat ggttggcaat ctccatatcc agctcagcca agaggcgttt
    23761 ggctactgca ccaactgcca cccgcatggt ggtttcacga gctgatgaac gctccaaaga
    23821 atttcgcaaa tcatcaaaac ggtacttaat ccccccaacc aaatcggcat gacctgggcg
    23881 aggatgagta attttccgct tgcttttaag gcggtcttca atgtcctccg cagacatgat
    23941 gtccagccat ttctggtggt ccttattgat gacatccata gtaataggcg cccctgtcgt
    24001 cttcccgtgg cgaacacccg aagtaaagac aacctggtca ctctcaatct tcatacgacc
    24061 accacgaccg tagccaccct gacggcgtct aagatcctca ttgatatcct cagctgtcaa
    24121 tggaagtcca gctggaattc cctcaataat agctgttaga cgggggccgt gtgattctcc
    24181 tgcagttaaa tatctcatac actctcctta ttttaccaag tagtctttca tctcttccag
    24241 agaaactggg tgaatggtcg ctgaaccaag ctctggcacc aagaccaatt tcaaggtgtt
    24301 accacgcgct ttcttgtcat gagtaagagc ctgataaagc ttgtcaactt tccaattttc
    24361 atagtcaaca ggcaagccga atttctgaca catctctgtg atagattggg taatgccagc
    24421 tggcatgagg cctttttcct cagcaacctt ggaaatctgt accattccca tggcaacagc
    24481 ctctccatgc atgaccttgc cataaccggc agtcgcttcg atggcatggc caatagtgtg
    24541 gccaaaattg aggtaaagac gaataccatt gtccaactca tcttcaacca ccatcttgcg
    24601 cttcacctga caagaatgtt caatcaaggt ctctgcatgt tccaaaatac tctcaacaga
    24661 accattcagt cccgtcaaga gagcccacag ttctggatcc tcaatcaagc catacttgat
    24721 aacttcaccc atcccttcaa tcaactctct ttttccgagg gtttcaagaa caagtggatc
    24781 aatcagaacc ccatctggtt gggcaaaggt ccccaccata tttttagcaa atggtgtgtt
    24841 aacgcctgtc tttccaccga tagaagaatc aacctgagct gtcaaactag tcggaatctg
    24901 aacaaagtga ataccccgca tataggtaga ggctacaaag ccagccaggt ccccaacaac
    24961 gccaccacca agagcaacga ttccatcgct acgagtcaga ccttgcttga ctagaaattc
    25021 atagactttc tgaacagtag ttaaattctt tctttcttca ccttctaaga aatcaaaaac
    25081 agctacctga aaaccagcat cttctaggct gagcttgacc ttctctgcat agagagaggc
    25141 tacatggtta tctgtcacaa tgactacctt ttgcggttgc cagagttctc gcaaccactg
    25201 accagcctgg gccatacaac ctttttcaat ctgaatatca taaggatgat gaggaatatc
    25261 gattctgatt ttcataggag agtctccctt tctttattgg tatttttctg ttaaagactg
    25321 ccaaatctct tctgtcggca tttccttgcc tgtccacagt tgaaaagctt ctgcagcttg
    25381 atagagcaac attcccagac cattgactgc tggattgccc tgacttctag cccatttcaa
    25441 aaacggtgtt tcaaagggtt ggtatatgat atctgcaact aaaagagttt ctggtaagac
    25501 tatgttttca ggaacaggag aggattggcc atccatgccc acactggtgg cattaactag
    25561 caaatccgac tcggcaatcc ttgcttgcag ttcagaaaca tattctaaag cacacaaatc
    25621 cactttaaag cctgtctgct cctgtaactt gtctaggtaa ggtcttgttt tttccataga
    25681 aacggaacga acaaagaccg aaatctgact gacgccatcc aaaatagcct gtgccaagat
    25741 tgatttagcc gcaccacctg cacccagcag ggtcatcttt ttacctgaaa ttgtaaaaga
    25801 aggcaagcac ttaaaaaatc ccttgccatc tgtattatat ccaattaaat tgccattctc
    25861 attgacaacc gtattaaccg caccaatcaa gcgcgcttca tcgctcagct catccaaata
    25921 aggaatcacc tgctccttat agggcatgga cagattgatg ccaaacatct ggtagcgacg
    25981 aatattggcc actgtttctg ccaagtcact cgcttcaatc tcccaagcca cataagcacc
    26041 gttggtagct gtcgcctcaa aggctctatt gtggatgaag ggagaaatag aatgcttaat
    26101 aggattggca acaactgcag ttaaacgtgt atagccatca agcttcatcc aaaatctccc
    26161 tgattttttt catgctagct agagaaatct gcccaggggc actagcctca tccagactgg
    26221 caaaagacca actcgaacca gtcacatccg cagtgatacg agagaccttg cccaccttac
    26281 ccatagaaat ggtcacatat tcctgttcag gattgagggt tttaaagcct cgtgtatagt
    26341 tcatcaagtc taagacatcc tgctccgtgt gagccatcac cgcaacctta acaagttttg
    26401 gatttaggat cgtcaactct gacaagattt ccatcatgtt ctcaggtgtt tcttggaaat
    26461 tatggtaact caaaacaaga tttgggaagt ccagcatttc ctcaaaaaca tccttgtagc
    26521 tatagtactc aaaatcaata tagtctggtt gatagagttg cgcaacttcc ttgattagat
    26581 ggatatactc ttctggagaa aggtcgattt ctccaccttc ggagcgagtt cgtagcgtga
    26641 aaaccaactc acggcctgcg aatttttcaa aaatggctgg agctacctgc aaaatcgctt
    26701 ctttaggcag atagtcggca cgccattcaa tgatgtcggc atccaggtac ctcgtggcat
    26761 ccagagcctg agcctcctct aaacttcttg gcattactga aacgattaat ttcatttact
    26821 aaccttcata ctaatcacct tgaggtaatt actactttca tcttttttat tataggcaaa
    26881 atctgctgga agaccatatt tgtttaaaat ctggtaactt cttcctgcaa agcctttatc
    26941 aatttgttct gtaaatttct ggcgggaaac attggcagca ttggtactgg caatgataat
    27001 ccctcccgga tttaaaatct caagactctg ggaaatcaac ttgtgataat ccttggccac
    27061 agagaaagtt tgttttttat tccgagcaaa gctaggcgga tctaggacaa tcacatcgta
    27121 ggtcaagtct ttgcgtttgg catatttgaa atactcaaag acatccatga ctataaaacg
    27181 atgctcgtct gtgctgagcc catttgcctg aaaatgcgct tgagacaatt cttgtgaacg
    27241 tttggctaga tcaacagaag ttgtctggct agctcctccc atggccgcag ctactgaaaa
    27301 agccgctgtg taggaaaaca tattgagcaa ggatttaccc atagccaagc cgtcaactaa
    27361 actaccgcga acctcatgct ggtctaggaa aattcctgtc atcaagccat cattcataaa
    27421 gacttgatac aggacaccat tttctaaaac agtgaaaaag tcaggtgctt cttgaccata
    27481 aacatgggca gattcatagt caaaaccctt aaagcggatt ttctcataag cccctaaaac
    27541 ctcagggaaa acctgtctaa aggcttctga tatagtctga cgaatctgat aaacataaga
    27601 gttataccaa gaaaagacgg cgtagtcgcc ataaaggtcc actgtcagac ccccaaagcc
    27661 atctccctct tgattaaaga gacgaaaggc agttgtcaaa tcatcttgat agtaggcgtt
    27721 tctcttttct ttggcttttc taaacaacgt ttcaaagaaa gtttgattga aggtcacctt
    27781 gtctttgctg acaaaccagc ccaagccctt gttttgctga gaaaggtagg cagtcccaag
    27841 aaagtttcct tcctgaccct gcacctctac ttcctgatcc ttaagattga cattctcaag
    27901 atcactggct tctagtaaaa ctagcccctt agcaagcttc ttttcaaccc ttttgctgac
    27961 tcttattcta ttcataacta ccattatatc aaacttttag acaattctca aaaaagaaac
    28021 tacccttgct tttttactct tcttttaaaa aatggtatac tagacttcct gcaaaactag
    28081 gaagtaaatg tgtaagaatc acaataaaaa atgctcttcc gtcttggagg agcatttctt
    28141 tttatcaacg aaaatcaaat agcaaactat gaaactagcc tcaggttaac tgtgagatta
    28201 taggtagaga ggttgtatca gcaatatgtg tctgtcaaat ttagtgacaa aggtagtaga
    28261 agaaagatga aaaaataaat cagcttcagt agatatctgg aaaatttgat tttatagaaa
    28321 agccttttgt tacaaactaa atatactatc aataaataat attatagaag caacaataat
    28381 tataatttca cctatttgca tcattctatt tcgaactcta aatatatgtt ctatcaaaaa
    28441 tacttgaaaa agacacatta taggaattaa cgtttttgaa attgaaaaat atccaaataa
    28501 ataaactata aacaacaaaa atagaactat gttatatttc ttattcaaaa cattcctccc
    28561 tatatatttt tgattaccaa tcttaatcat ttacaactac attctaacaa actataaaag
    28621 cgtttgtcga attgaattta tcaagcaagc gaccaaccag ctcatctttt ttctatttct
    28681 gccaatatgc gtgacaggta ataatgatag ccaaaaatag caagagcaag caggaggata
    28741 agagctccta ctcccaagtt gatggcaagg ataggggaga gagactgaac caagaatatg
    28801 ctcccaatta caagggccat caggattgca ctataaataa acaataaaac tatggcgact
    28861 atgccatttg aacgattcac caggtccgta atgctactcc aattggttga cagattttta
    28921 acgtccttaa agtaatggtg gcaagaaagg atgacactgg caatgatcca gactacaaga
    28981 aggtaaatca tcgaaaggat ggacaagcct agatatagag aaagaccgag caaagtcaga
    29041 actggtaaaa aggactgaac agcaaagata atccaaaatt tcactttcac ataacgagca
    29101 aagtcaaagg gtaaactctt cagaaaatca acattttccc tctccaagga caaggcaatt
    29161 gaatgcaggc tggtgatatt gttattgaca actgctataa acactcctac aaaaaacaag
    29221 ggtaaccagt atggaggatg aatgtctgga actatctgag aatctcggat tttggaaatc
    29281 agactgatca tcatgagata aggaaggaaa gcacttgtaa aaagcactgt aatcacgcca
    29341 gtcccctgtc ccaagagggt gaggtggtag cgtaaaacca tacggaaaaa gcccttttta
    29401 gtagttgaaa tcctctcctt gctgcgacgt tcttttttga ccttctcctc actgttaagc
    29461 aggatcacgt cataaaaacg aggaaggacc ttctttttgg tcaggtaaag caagaaaaca
    29521 gttaaagcta tccaagcgag cagacccaat atggcttcta ttgaaaaagg ctccactgct
    29581 attttgtaaa agatatgaag aggataaagg agaaatggaa tgtctctaac tttgtcaaca
    29641 atacttccaa aagtcgactg tagaaagaag acaaatatta aaggtatgag aactcctatc
    29701 ccaatcatca cattcgaaaa aatagactga tactttctga agaccctagt ttgagccaag
    29761 aaatgcactg ccactaccat cactagagcc acagagacaa ataataaggt caaggacagt
    29821 agcatcaaag gcaaacccag ccatagagaa ggagctagcc taatgtagag gactagaaaa
    29881 taagctagga ttggtacaat tccagttaga gctggcaaga ggacagacag tcctttagca
    29941 attataatct ctgattcttt aaaggcatag ggcctatacg ataccaaatc cttactctca
    30001 taaaagacat tgtaaaaggc cgttaaagaa gttgaaaagg caatcactag taaaatagca
    30061 accatcgaac taaaataaat aggcatttcc tcaaaaggaa aatgaatggc tatattacta
    30121 aaacagatga gcatcaagag actggaaaaa atgtaagaac ttaggactct agcggaaaca
    30181 tttacttttt tcccaggatt cttagcctgc ttctttcgta gattaaccag attagcttct
    30241 tgagatgaat aaaggatatt gatatcaact aattttttaa tgaccttgag acgcatccgc
    30301 aacctcctct tttctaccag caagactaag gtagatactt tccaaagact gggctgggta
    30361 atcttttctc aagtcctcta cactaccaca ataaatcaaa tgcccctttt tcaaaatggc
    30421 aatccgatca cagacttgct ctgccacctc taggacatga gttgaaaaca agactgtctt
    30481 ccctttttgt gcatgttcct tcatcatctg tttcaaatca aaggcagcct ggggatccaa
    30541 accagtcaag ggttcatcca agacccaaat atcgggatca gacaagagtg ctccgatgac
    30601 aaagactttc tgacgcattc cgtgagaaag agtttcaata acctgatagc gattttcagc
    30661 aaaatcaaaa acgttcaata gcctagctag actagtctcc aagtcagagc tagtcagatc
    30721 ataggatgaa gcgatcaatt cccaaaattc attggccgtt aagcgtaaaa ataagtcagg
    30781 cgagtctgct acgtagccaa tctttcgttt gatcgccaag cgattttccg ataactcctg
    30841 accgtctacc aaaatacgac cactgctggg tgaaatgata ctgactaggg attttatggt
    30901 ggtcgatttt ccagccccat tatgaccaat caagcccata atctctccat tttcaatctg
    30961 caaattgaga ttactcaagg cctctttatc accatacaac ttactaacat tttgaaattc
    31021 aatcatggga caactcctat ctttatctgt attatacatc gctatactag cactttgata
    31081 caaaaaaatc aatactttat tttaagtagt taaaatagtt tccttctatc ttacgcaaac
    31141 gtttgcgccg agagatactt tatgatagaa taaagaacaa gattgacaag taagaggaaa
    31201 catcatgcaa aatcaaacac tcatgcaata ctttgaatgg tatctgcccc acgacggtca
    31261 acactggacg cgtctggctg aaaatgctcc acacctagct catctgggga tcagtcacgt
    31321 ctggatgcca ccagccttca aggcaaccaa cgaaaaagat gtcggctatg gggtctatga
    31381 cttatttgac ttaggagagt tcaaccaaaa agggactgtc cgcaccaagt atggtttcaa
    31441 agaagactat cttcaagcca ttcaagccct taaagaacag ggaattcaac ctatggccga
    31501 tgtagttctc aaccacaagg ctgctgccga taacagggaa gcctttcagg ttatcgaagt
    31561 tgatcctgta gaccgtacag ttgaacttgg agaacccttc accatcaatg gctggactag
    31621 ttttaccttc gatggtcgcc aagataccta taatggcttc cactggcatt ggtaccactt
    31681 caccggtaca gactacgatg ccaaacgcag taaatctggg atttatctga tccaagggga
    31741 caacaagggc tgggctaacg aggaattggt cgataacgaa aacggaaact acgactacct
    31801 catgtatgcc gacctagact ttaaacatcc tgaagtcatc caaaacatct atgactgggc
    31861 tgattggttc atggaaacga ctggtgtagc tggtttccgt ttggatgccg ttaagcatat
    31921 tgactctttc tttatgcgca acttcatccg cgatatgaag gaaaaatacg gtgacgattt
    31981 ctatgttttt ggtgaatttt ggaacccaga caaggaagcc aatctggact atctcgaaaa
    32041 aacggaagaa cactttgacc ttgtcgatgt tcgtctccac cagaatctct ttgaagccag
    32101 tcaagctggc gcaaactatg accttcgtgg cattttcaca gatagcctgg ttgaactcaa
    32161 gcctgacaag gctgtgactt ttgtcgacaa ccacgatacc caacgaggac aagcccttga
    32221 gtctaccgtt gaagaatggt tcaagccagc agcctatgcc ctcattttgt tacgccaaga
    32281 cggccttcca tgtgtctttt acggagacta ctatgggatt tcaggccagt atgctcaaga
    32341 agatttcaaa gaaatccttg accgcctcct agccatccga aaagacttgg cctatggaga
    32401 acaaaatgac tactttgacc atgctaactg tatcggttgg gtacgttcag gtgctgaaaa
    32461 tcaatcccca atcgcagtcc ttatctcaaa tgaccaagaa aacagcaagt caatgtttgt
    32521 cggtcaagaa tggactaatc aaacctttgt agatttactt ggtaaccacc aaggtcaagt
    32581 tacaattgat gaggaaggtt atggacaatt ccctgtctca gctagatccg taagtgtctg
    32641 ggcagtcaat accatctaat agctcataat aaccaagcta ggtccaagcg gatttggctt
    32701 ttttgtattc acaaaaagac ctacccaaat ggatagatct ttacttgatt acaatttacc
    32761 tgctactgca tccaacaatt cttggatctt agcttggttg cttcctcctg ccatggccat
    32821 gtctggttta ccaccaccac gtccatcgat gattggtgct aattctttga caaggtttcc
    32881 tgcatgaagg tcttttgtct tgcttgctac aaggacattg actttgtcac cgatagcggc
    32941 aactaggaca agaagatcag agtagtcttt ttgtttccag ttatctgcaa aagtacgaag
    33001 ggcaccggca tcggatacag acacttgact agcaatgtaa cgatgaccgt tgacttcctt
    33061 agcatctttg aagatatcgc ctgcggctgc agctgcggct ttttctttca actcagcatt
    33121 ttctttttga agttgacgaa gttgttcttg aagtccttct accttgtgag gtacttcctt
    33181 gacttgaggt gctttcaaga ttgctgcgac agctttaaga gcatcctctt gttcacgata
    33241 ggcttcaaag gcttccttac cagtcactgc caagatacgg cgagttcctg aaccgattcc
    33301 ttcttctttg acaattttga agagaccaat ctcagaagtg ttgccaacat gagtaccacc
    33361 acaaagttca atagagtagt caccgatagt cacgacacga acttccttgc cgtatttctc
    33421 accaaagagg gccatagctc ccatttcttt agcagtgtca atatccgttt caactgtctt
    33481 aacttcaaga gcttcccaga ttttttcgtt gacttgctgt tcaatcgcac gcaattcttc
    33541 agcagttaca gcttggaagt gggtaaagtc aaagcgaagg aattcaactt cgttaagaga
    33601 tcctgcctgt gttgcgtggt ttccaaggat attgtgaagg gcagcgtgaa gcaaatgagt
    33661 cgcagtgtgg tttttcatga cacggtgacg gcgattgcta tcaattgcca aggtatattc
    33721 ttggttcaag gcaagcggtg caaggacttc aactgtatga agggcttgac catttggggc
    33781 tttctgaaca ttggtcacag tagccacaac cttacctgac tcatccaaga tttgtccgta
    33841 gtcagctacc tgtccaccca tttcagcata aaatgacgtt tccgcaaaga taagagaggc
    33901 agttccttct gaaacagctt ctacttctgc attgtccgcc acgatagcta ccaatttaga
    33961 agacaattgg ctagcattgt agttgaagac actttctaca gtgatgtttt gaagagtttc
    34021 attttgcata cccattgagc cacccttgac agctgaggca cgcgcgcgtt cttgctgttc
    34081 tttcatggct gcttcaaaac cttcacggtc tacagtcata ccagcttctt cagcgatttc
    34141 ttcagtcaat tcaactggga acccataagt atcatagagt ttgaagacat ctgaaccagc
    34201 gataacagat tgaccttttt ctttcaagtc tgctacaatg ccttgggcaa agtgttgacc
    34261 tgagtgaagg gtacgggcaa atgattcttc ttcgctctta acgattttct caataaagtc
    34321 acgtttctca agcacttctg ggtagtagct ttccatgatt tttccaacag ttggaaccaa
    34381 tttgtaaagg aaaggttcgt tgatacccaa tttttgacca tgcatagaag cacgacggag
    34441 aagacgacga agaacataac cacgaccttc atttcctgga agggcaccat caccgatagc
    34501 aaatgaaaga gaacgaatgt ggtctgcgat aaccttgaag ctcatgttgt cgccatcttg
    34561 gtcataaacc ttaccagaca atttctcgac ttcacggata atcggcatga agaggtccgt
    34621 ttcaaagttg gtcttagccc cttggataac agccaccaaa cgctccaaac cagcgcccgt
    34681 atcaatgttc ttatgtggca attccttgta ttcgctacga ggaacagcag ggtctgcgtt
    34741 aaattgtgac aaaacgatgt tccagatttc aatataacgg tcgttttcaa tatcttctgc
    34801 aagcaggcga agaccgatat tttctgggtc aaaggcttcc ccacggtcaa agaagatttc
    34861 tgtatctggt ccagaaggtc ccgcaccgat ttcccagaag ttgtcctcaa ttggaatcaa
    34921 gtgacttgga tccactccca cttcaatcca gcggttgtaa gaatctttat cgtctggata
    34981 gtaggtcatg taaagttttt cagcagggaa atcaaaccat tcagggcttg tcaaaagctc
    35041 ataagcccaa gtgatagctt cgtcacggaa gtaatccccg atagagaagt tccccagcat
    35101 ttcaaacatg gtatggtgac gcgcagtctt ccctacgttt tcgatgtcgt tggtacggat
    35161 agccttttgg gcattggtaa tacgtggatt ttcagggata atggtcccgt caaagtattt
    35221 cttaagggtt gctaccccag agttgatcca caaaagagtt gggtcattta caggaaccaa
    35281 acttactgat ggttctactg agtgagcttt ggtcgcccag aaatcaagcc acatttggcg
    35341 tacttgtgca ctagatagtt gtttcatatt gtctccttat ttacttgttt aatgtgattg
    35401 gctttccagc atttccacat agtcaatcgc gacacagagg gaaatgacta ggtctgcata
    35461 agcgtcctca agaaccgtta cggtataggt agaggtcaga tggaagagtt ccttcttaat
    35521 ttccgcaatc agctgatcgc gatcatccag caatttgaaa ttcaaatccc agatattgcc
    35581 ctcgatacga agacctagat tatcaaactc atacttatct cgccagaagg tcaacttctt
    35641 acgaatgaca aaactcgagc catcccgaag ctgaatctca aaacgaggaa gcaaggtcaa
    35701 gatttcttta ctaatctcac tgacttgttc accagccgca tcatagatgg taaaggtttt
    35761 aggaatctta aaaaatgatc cctccacctg ataggcaatt tctcccctgt cattcttgat
    35821 agcgaagcgt tcgcctccaa gacgaaactt ttgtttgaca agaaatgttt tcatcaacac
    35881 ctccaaaaat caaaagacaa gctcatatca cgaagggcga aaaaccgcgg taccaccttc
    35941 attcaatgaa cttgtcattc tcttgttctt atgcaattgt atgattgagt agcatgactt
    36001 cctagcttag atggctcgca gcaccgccat ttctctggac taagacaagt gaaaatcaat
    36061 tctcaacttt cttattataa cgttttttta agcttgcgtc aactggaaat gatctccgtt
    36121 gaattagacc aattccctac atctctgatt actttttcag gatatatttt ttcttactgc
    36181 cgtttttctt tttatcccaa attttcatat tactaaacac agctactaga atatttccaa
    36241 atataaaggt gcctatcacc caatatatgg actcagttgt taggtattgt cgatccaagc
    36301 catcctttaa atggaatagt ataacagttt ggttaacaat cataaaggtt ggccagaaac
    36361 tttttttgaa aaaagtagac attttcatta tttgttgccg ctttctgtaa ggttaatact
    36421 caataaaaat caaaaagcaa actaggaagc tagcctcaag ctgtacttga gtacggcaag
    36481 gcaacgctga cgtggtttga agagtatagg cttagtatac tactaggcaa gcaaataagc
    36541 aaataaacaa ataaacaact agaatagaaa aagatagggc tctaaaaact gacttctatt
    36601 ccttaaaaac gaaccagctt gactgattcg tcttcttacg tttatctcct acttccgata
    36661 cattttaaac tgtaggaaga ggtcgctata tttccctgtc catttatggt caaatttctc
    36721 ataaacttct aggtgtttca tggtttcaac atcgggatag aaggccttat cttcctttgt
    36781 ttcctctggg agcaattcct tcgctggtag gtttggtgtt gaatagccga catactccgc
    36841 attttggaga gcattttcag gtttcaacat aaagttgata aaggcatagg ctgagttttg
    36901 gtttttaact gttttgggaa tgaccatatt gtcaaaccaa agattgctgg cctctgtcgg
    36961 taccacataa cgtagatttt catttttttc taacatttgg ctggcttcac cagagaaggt
    37021 cacgccgatt gcaacattat tctgaatcat atagcccttc atctcgtccg caacgatagc
    37081 cttgatattt ggagtcagtt tgtagagctt atccactgtc tcttccaact gctgcagatc
    37141 cttggagttg aggctgtagc cgagggaatt gagtcctagt cccagcacct cacgcgcccc
    37201 atcaaagagc atgatagaat tcttatactc cggcttccaa aggtcatccc aatgctcagg
    37261 cgcttcatct accatggttt cgttgtagac aattcctaag gttccccaga agtaagggat
    37321 ggagaattta ttacctgggt caaaggactg gttgagaaac tctggtccga tattttcgat
    37381 tcctttaatt tttgaataat caagcggaac caagaggtct tcgtccttca tcttgttaat
    37441 catgtattca cttggaatgg caatatcgta ggtcgttcca ccctgcttta tcttagtgta
    37501 catggcttcg ttggagtcaa aagtctcgta ctgaacttga attcctgttt cttctgtaaa
    37561 ctgagtcaag agttcaggat cgatatagtc tccccagtta tagataacca atttttgact
    37621 atctcgacta ttgattttac tatctaaatg agtcgcaatt ccccacaaga caaggataat
    37681 cgctgcaatt cctgctaaaa atgaatagat ttttttcatg cttgctcctc cttctcacga
    37741 gagataaagt aataacctac aactaggata atactaaaga gaaagactag agcagacagg
    37801 gcattgattt ctaaggaaat ccccttgcga gcacgagagt aaatctcgac tgatagggtt
    37861 gaaaagccat ttcctgttac aaagaaggtc acggcaaaat catccagcga ataggtgaag
    37921 gccatgaagt aacctgcaat gatagacgga gtcaggtaag gaagcatgat ttccttaaac
    37981 atctgaaatt gactagctcc caagtcatag gccgcatgaa tcatgtcgcc attcatttcc
    38041 ttgagtcgag gcaagaccat caagaccacg ataggaatgg agaaggccac gtgactagat
    38101 agaacggtca aaaagccaag tgaaaacttg agttgggtaa agagaatcaa gaagctagca
    38161 ccaatcataa cgtcaggcgc aaccatgagg atattattga gtgatagaaa ggcttcttgg
    38221 tatttcttac gagactggta gatgtaaatg gcaccaaaag tcccgataat ggtcgctatc
    38281 aaggctgata ggaaggccaa gaaaaatgtc tgagccaaaa tcagcatgag tctcccatct
    38341 ccaaacatgg tttcaaagtg agtccagcta aaacctgtaa agctattcat atcatcacca
    38401 gcattaaagg catagccaat caagtaaaaa atcggcaggt agaggaccag aaagaccagt
    38461 cccagataaa ggttggcaaa ttttttcatc gttctctcct ttccttggtc acccacatgg
    38521 tgatgaacat ggtcaggatg agaatcacac cgatggttga acccatacca tagttgtcat
    38581 tggttagaaa attctgctca atagccgtcc ccaaggtgat aacgcggttc ccaccaatca
    38641 aacgggtcag catgaagaga ctcaaacttg ggataaagac cgactgaacc ccacttcgca
    38701 caccgttcat agatagaggg aagatgacat gacggaaggt ctcccactta gttgcaccaa
    38761 ggtcgtaact agcattgatg agattattat ccatatcgtc taagacattg aaaatcggca
    38821 aaatcataaa ggggagctcg atgtagcttg cgacaaagat aaaggaaaaa tcggtaaaaa
    38881 gcaactgttg tgaaccaatt ccgataaatt ccaagaattg gttaatagag ccattttgac
    38941 caaaaatccc gataaaagca taggctttaa ggagcaaatt gatccaggta ggaaggataa
    39001 tcagcatgag ccagagttga cggtgcttga gacgggtcaa aaagagggct gttggatagc
    39061 tgataagcaa ggtcacaaag gtcacaatcc ctgcataaag cactgagttg aaactcattt
    39121 tgaggtaggt caagttttgt gacgcaaagt aagatttata attttctaaa ctaaactggc
    39181 cttcaatgtt gaaaaaggat tgtccgaaaa tcaagaccaa gggtgccaat acaaagaggg
    39241 caatccaaag catgtagggc actacaaaga gtttagagct tgttttcttc atctctttcc
    39301 tcctcgatcg cattgatcag acctgcttct tgctcttcga tttccacgta ttcttcgata
    39361 cgagcatcga actcttcttc ggtttcattg agacgcatga tgtggatgtc ttctgattca
    39421 aagtccagac cgatttcctc acccacgatg gccttacggg ttgagtggat catccattca
    39481 tttccaagtt cgtcataggc gataatttca taatgaactc cacggaagag ctgggtgtcg
    39541 accttaactt ggagtttgcc ttcttcagga agggtaatgc gcaagtcctc tggacgaata
    39601 acgacctcaa caggttcatt tggcttcatc ccaccatcaa ccgcttcaaa gcgtttgccg
    39661 ttaaattcga ccaagtagtc ctcaatcatg gtacctggca agatgtttga ctccccgata
    39721 aaggtggcaa caaagtggtt gattggctca tcgtagatgt ccacaggggt tccagactgg
    39781 acaatctcgc catcattcat aacgaaaatc cagtcactca tggcaagagc ttcttcctga
    39841 tcgtgagtga caaagacaaa ggtaatgccc aatcgttgtt gtaattcacg caattcgtac
    39901 tgcatgtctg ttctcaattt caagtccagc gctgataaag gctcgtccaa caagaccaca
    39961 cggggttggt tgatgatagc acgggcgatg gccacacgct gacgttgtcc tccagaaagt
    40021 ttgcggatgg aacgtttttc ataaccttcc aactgaacca tcttgagaac ttccgctaca
    40081 cgctgctcga tttctttctt atcaatttta cgcaagcgaa gtggaaaggc aacattttca
    40141 aacacattca tatgtgggaa caaggcatag gattggaaga cggtatgtac gtcgcgcttg
    40201 ttggttggaa tatcattgat acgaacaccg tctagcatga tatctcctgt cgtcgcatcc
    40261 agtaaacctg caataatgtt taggatagtt gatttccccg aaccagatgc acctagaagg
    40321 gtgtagaatt tcccttcttc caactcaaag ttgatgtctt tgagaacctt ggtgttgctg
    40381 tcttcaaaaa ctttagagac gtttttgaat tcgataattg gttttttcaa ttggcataaa
    40441 ttccttcttt ttcatagatt aaccgatcgg ggctctgtca ggtccccact acctcttgca
    40501 gggagtaaaa ccacctgcat acatcttcgc taccgatagg ctttcacccc ctttccatga
    40561 catagcagca gcttttcaaa tacagcttcc tacttgcttt cacccaagat ccggacttct
    40621 ctttcaagcg taatacctga gtgttccttg actttttcga taaccgattg gatcaagtcc
    40681 tcgtagtctt tggccgttcc atctgcgaca ttgatcataa atcctgcatg cttttctgac
    40741 acttctacgc caccgatacg atagcctttc aagccagctt ctgaaattaa ctgacctgca
    40801 aaatgcccga ctggacgctt aaagaccgag ccacaagatg ggtattccaa aggttgcttg
    40861 agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga taacctgatg ggttcctggg
    40921 gctagggcaa atttaactga caagacaact gcaccagact cctgaatagc tgaatggcgg
    40981 taaccaaaag ccaagtcttt agcagacagg gtttcgattt ctccatcctt ggtcaagacc
    41041 ttacaagact gcaagatgtg agcaatctcg ccaccatagg cacccgcatt cataaagaca
    41101 gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa agccagtcaa actatgacgg
    41161 agggcaatgc gagttgtttc aatcaagtta gccccagctt ctgcttcaat ggtatagcca
    41221 tcaacagaaa cgttattgag cttgtcacac aagatgacaa atccacgaat cccaccatca
    41281 cgaacgatga tattgcttgc attgccaaga accatccaag ggatattttc ttggttggca
    41341 aatttcacaa ggcgcgccaa ctcaaaacga tttcgtggaa agaccaaata atcagcctct
    41401 ccacctactt ttgtataact atagctatgc aagggttcct taaaacggat atcaattcct
    41461 tctaagattt caagcatttt ttctcttaca gacatgtcac tcttcctttt acaaaattca
    41521 ttccattata ccatttttag agacatttga cgaccataaa aataccttgt ttggattttg
    41581 cataagaaaa agaggttccc ccctttttat gattttttac aaaagatttc cttggttcca
    41641 taggcgacca gaacgagctc cagtgctaga atcacttcaa ccaagactgg atttgtcaac
    41701 cagcctactt ggaaaagaga tggtgccaga tcaaagaagg catgcaagcc ataggctgct
    41761 aggagataaa tccatttctt ctggcgaaca gcttggtaaa cccaaactgt caaaagtaat
    41821 tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat aaatctgcca gaccgaaagt
    41881 gactgaatgg tttttaacat attttcagac agtaattgca taacctgtgg attctgagtt
    41941 tgaactgccg aaagaacgat gtaaagattg agtaaactaa taaggcctag aaaaatcaac
    42001 tccaagccac catgccctag gccataggcc aaggcatctg ccttttccaa acttctcttt
    42061 ttctccaacc atttgaagaa aacaagacga gccgtttcct caaaaaatgc tgccatggct
    42121 aggccataga taatatagat aagcggatga tcttgcaaga gggcaatact accgtctttt
    42181 tgagggtgca aaatcaagat atgcaccagt ttttccaaaa tctgcgaaga gacaaagaaa
    42241 gcgactgcac ccaagcccaa gacagctaga ttaatctggt atttcttttt ggcaaaccaa
    42301 atgcttccta tcagaaaagc tagcaacagc aacatggtaa taatgatatg aatggtcatt
    42361 ttcttctcct attctgcctt ttcaatatct tttttcatct cgtcaacatt gaacttagca
    42421 aacaagtatt gacggtcttg gactgggaaa cgttggtcca actggtcaac tgctcccacc
    42481 tcgataatgc cttccttgat gacaaaatcc atgacatgtc caccgaaggt caaatcatct
    42541 gatatgaagt gcagatggta gcctgccaca ctgaccccat ggaaaatctc aggcgtccag
    42601 aaaccaacaa tagtccccgc aacattgtca cgactatatt ccggttgatg ggttgcgaca
    42661 tcagcaaact tagtatcggg tgttgactta ggaatcatac gcacatgcat atgtgaaaat
    42721 tctccccgaa tcttgataga acggaaaaga ttttccccat cataataaga ctcaattcgt
    42781 tctttcaatt ccttgtctgt catctcaaag cgctggcgga aaatgacctc tgcctgatgc
    42841 ggtaccactg cagcgtaagg aataagggca tctggtgaca cttccacaat ttctggctgg
    42901 tctcctgacc ctttggcctg ataagccttg ccatccaaga caatcaattc cccatcaata
    42961 gaatccaagg ttcccaaacc aaggtcacca tgctctagca attctcccac tgtcatggta
    43021 ccaccataaa ggccagccat caaggctcca agagtattgt attgaaataa tttcactggt
    43081 tcctgcacgt tcttatccat tcactttctt gtctgttatt ctataaatct tactcctaag
    43141 tataccacat ttgcccctag atgtgaacga gagaaacgct ctagacattg ccaagaagga
    43201 aaaaaaaggg tacaatgtaa caaaatcaag ggaggtctgg aatgaagaaa caaagcaagt
    43261 acaaagaggt cgtttcctat ctgaaaaatg gtatcgagtc tggacgattt ccgacgggta
    43321 gtcgcctacc ttctatccgt caactgagcc ttgactttca ctgtagcaag gacaccattc
    43381 aacgagccct gctggaatta cggcacgaac aatacctcta tgccaagccc cagagtggct
    43441 actatgtcct agaacaaggg caacaccagg acctagaaat cgaggttacc gacgaacatg
    43501 ccagtgccta tgacgatttc cgactctgtg tcaatgaaac cttgattggc cgagaaaact
    43561 acctcttcaa ctactatgat aatcaagaag gattagaaga cctaagacaa tccattcaca
    43621 aactcctctt tgagcaagct ctctactgca aggctaacca actagtactg acttctggaa
    43681 cccaacaagc cttgtttatc ctctctcaaa tatcctttcc tagccaagcc aaggaaatct
    43741 tggtggaaca gccaacctac catcggatga atcgcctctt gattgcacag gggctggact
    43801 atcaaacgat tgaacgaggc attgatggga ttgacctgaa ggagctggag cgacacttca
    43861 agacaggaaa aattaagttt ttctacacca ttccccgatt tcactatcct ctgggacatt
    43921 cctattctga gcaagacaaa cgatccattc ttaacttagc tgccaagtat gatgtctata
    43981 tcgtagaaga cgattatctg ggtgatttgg actccaagaa gggccaaacc ttccactatc
    44041 ttgatacaga ggagcatgtc atttatatca agtccttctc gaccagcctt tttcctgccc
    44101 ttcgtattac agcactcatt cttccaaatg ctatcaaaga agcatttgtg gcctacaaaa
    44161 atatcctaga ctacgacagc aacctcatta tgcaaaaggc cctgtcactc tatattgaca
    44221 gtcaattgtt tgaaaaaaat cgtttggctc gcttgaccaa tcatgaatct taccaaaaac
    44281 aaatcgagga aaggataact aaaacacctt gtccccttcc tcattatccc ctacacgatg
    44341 gtttattgct agacctgaga cagtatccta aaatcgccag tctcaaacac agtcaaccgg
    44401 gcttggactt ctttgaagag gcctatttaa gtacctgtcc ttatcaattt gccaaggtgt
    44461 ccctagacaa tctggaaaat gttttaaact atttaaaagc agaattggaa tgacatccaa
    44521 ctctgctttt tcttattctt caacttcagt tagacttgct gctttttcaa gatagctttg
    44581 gtaaattcct tcatccttaa gtttttggat gaccttgtct accacttctt tcaaatcatc
    44641 actatttttt ctaagagcaa cggcattggc gtcgccgtcc ttcatcttca agctgacagt
    44701 tgcgacagct aagccagcgt ttttagcagc ataactaagt gcaacaggct catccatatg
    44761 aacagcatct acttttccag cctgcaattc attgactgct tcacccatat tagttaggga
    44821 agttaattga gcttttggca attgttcctt gaccattgat tctggaacag tccctttttg
    44881 ggctgcaata ttagcacttt ctaggctagt taaatccttg tatttttcca catcagcctt
    44941 acgaaccaag aaactaatct tgttttcata gtatgggatt gaaaaatcaa agacttcttt
    45001 tctctcgtca gtagcactaa ttcctgcaac tgctaggtca gccttaccag tttgaagact
    45061 ggtcaaaaca ttgtcaaaac tcatgcttga gatttccaac ttaaccccaa gttcatcagc
    45121 gatagcctga gccatgtcga tgtctgcacc gactacctgg ttctttccat caaccaatga
    45181 ttgaaattca aagggtgcat agtcaggact ggttgccaca actaatttcc ccttttgctt
    45241 aattgcctca acagctgact gagaaccatt agaacctgac tggcaagcta ctaagaagaa
    45301 acttgcaaga aaattacata aaacaaatat ccattttttt gatttcataa ataaacaacc
    45361 tctctgttaa ttttgtataa ttataacgat atccaagtta cttgtcaagt gttttttaaa
    45421 tttttatctc aaaaatattt tttcgttcaa aaaaaggagc catcagttga tttcaagctc
    45481 ctttttatac agaattaaac tattttatag ttcgacaatc ttacctgttt caaagtagac
    45541 aacccattca cagatatttt tagcatagtc accgatacgc tccaagtagg aaataacttg
    45601 gaaataatca cgacccgtaa caatggcttc tggatttttc ttaatctctt cagtcgcaag
    45661 gtcacggata gtttcaaaat agtggttaat ttgctcatcc atggaggcca cccggtatgc
    45721 gtcgtcaaca gaaccattaa gataaagatc aagtgctgct tcaacaacgc ttttaacttc
    45781 acgtcccatt tttttaattt cttcctctac agctggaata cgttcttccc ccttcatacg
    45841 gatggttgcc tgggcaatgg ctacagcgtg atcccccata cgctccacat ctgatacagc
    45901 cttaaggaca gtcaagactg tacgcaaatc ttgagagact ggttgttgga gtgcgatcat
    45961 ttcaaatgat ttcttttcca gtttcacttc gtattcattt acttctgcat catcttcgat
    46021 gacctctttt gccaggtcac ggtcatgcgt gacaaaagca cgtaccgtac gattgatttg
    46081 tgagagcact tcttgtccca tagcgtagaa ctggttatgt aatttctcta aatcttcttc
    46141 aaattgagat cgtaacatct ttcatctcct tatccaaatt ttcctgtaat atagtcttcc
    46201 gtttccttgt gttggggatc aaggaacatc tgcttggtat cattaaattc aatcaaatct
    46261 ccatctagga aaaatcctgt cttatcagag atacgtgaag cttgctgcat ggaacgggtt
    46321 accagaagca tggtgtactt gtcttttaga ccatacaagg tttcctcaat tttaccagct
    46381 gaaatcggat ccaaagccga agttggctca tccaagagga tgattttagg actagttgcc
    46441 aagacacggg ccacgcagac acgctgctgt tgaccacctg acaatccaat agctgaatca
    46501 tatagacgat ccttgacctc atcccagata gaggcacctt gcaaggcttt ttctacggct
    46561 tcatccagaa cctgcttatc cttaattcca ttgatacgaa gcccgtagac aacattctca
    46621 tagatagtca tagggaaagg attaggttgt tggaaaacca ttccgatttc cttacgtaat
    46681 tcaaccgtat ctgtacgcgg actgtagatg ttgtgaccat tgtacaccac ggatccagtt
    46741 gtggtcacct ctggattgag atctcccatg cggttgagag acttgaggag ggttgacttc
    46801 cctgatccag atggaccaat caaggctgta atttccttag gttggaaaga tagggaaaca
    46861 ctattcaaag ccttcttttt attataataa acggacaggt ctgatacctg taaaatcgca
    46921 tctgtcatac ggtttccttt ctaaccaaag tgaccagata catagtcatt ggtggactgt
    46981 agcttggcat tttggaaaat agttgcagtc ttgtcatact caatcaaatc acccaagtaa
    47041 aagaagcctg tatagtcact tgcacgagca gcctgctgca tattatgcgt tacaatgatg
    47101 atggtaaagt ttttcttgag ctcaaacatg gtctcttcta gttgcatggt cgcaatcgga
    47161 tccaaggctg aggctggctc atccattaag aggatatctg gcttaacaga gatggcacga
    47221 gcgatacaga gacgttgttg ctgaccacct gataaggtca aggctgactt gtggagatcg
    47281 tctttaacct gatcccagag ggcagcctga cgaagggagg tttctacgat ttcatctagg
    47341 acttgcttat ccttaactcc agcacgttca tgcgcaaagg taatattacg gtaaattgac
    47401 ttagcaaatg gattggggcg ttgaaaaacc attccaatgt gtttacgcat ttcataaacg
    47461 ttgatttctg gacggttgac atcaattcca cgatagagaa tctgcccagt tactttagca
    47521 atatcaatgg tatcattcat gcgattgaga ctgcgtaagt aggtagattt ccccgatccc
    47581 gacggaccaa tcaaagctgt aattttattt ctttcaaatt gcatatcaat ccccttaatg
    47641 gattcatttt taccatagta aacatggaca tccttagtag aaagggctac tttttcttca
    47701 ggaaaggtaa ggatatgctt ctcatcccag ttatatgttg acatggcttc tcctttaggc
    47761 agcggttaat ttcttgtgta gatagattcc gaacttacga gctccaaagt taaaaatcag
    47821 gataaagatc aggagcacag cggcagaacc tgctgataca atggttccat ctggaatagt
    47881 gccttcacta ttgactttcc agatatggac agccaaggtt tctgcttgac ggaagataga
    47941 gatggggcta gtcacactga ggatattcca gttagaccag tcaagagctg gcgccgattg
    48001 ccctgctgta tagatcagag ctgcagcttc gccaaagata cgaccagatg ccaagacgac
    48061 acccgttaca atacctggaa gcgcttctgg aataacaaca tgaaccactg tctcccagcg
    48121 agaaatccca agagccagac cagcctcacg ttgggtatgg tgaacgtgtt tcaaactatc
    48181 ctctacatta cgcgtcatct gaggcaagtt aaagactgtc aaggccaagg cacctgaaat
    48241 gattgaaaac ccatactcaa actggactac aaagatcaag taaccaaaga gacccaccac
    48301 cactgatggt aaagaggaca agatttcaat acaagtccgc acaaagttgg taacaggacc
    48361 ttttttagca tattcagcca agtaaatccc agctcccata gaaagaggta cagaaataat
    48421 caaggtaatg accaatagga aaaaggaatt gtaaagctga atgccaatcc caccacctgc
    48481 ttgaaaagca gaagaccttc cagtcaagaa agaccaagag atatggggca agccccgaac
    48541 caagatatag agaattaagg aagccaagat tgttacaatg atgctagcaa tcgtatagag
    48601 gacagctgtt gcaagtttat ctaatttctt agcgcgcata atttttcttt cctctttctt
    48661 tcgtaatcaa tttaatcaca ctgttaaaaa ctaaactcat caagagcagt accaaggcca
    48721 gtgaccagag aacattatta tttacagttc ccatgacagt gttcccaatt cccatagtta
    48781 atatagaagt taaagttgca gctggtgtgg tcaaggaagt tgggataaca gctgagtttc
    48841 cgacaaccat ctggatagct aaagcctcac caaaggcacg cgccatccca aagaccactg
    48901 cagtgaaaat accagaacgg gccgccttca agatcacacg ccagatagtc tgccagcgag
    48961 tggctcccat agcgaaactg gcctcacgat aataacgagg aaccgcacgc aagctatccg
    49021 ttgtcataaa ggttacggtc ggcaaaatca tgacaaagag gacggaaatc cctgacaaaa
    49081 tcccaaaacc agtcccacca aagacactgc gaacaaaggg aacgacgact tgcaagccaa
    49141 taaatccgta cactactgaa ggaatcccaa ccaggagttc aatagctggt tgcaaaatct
    49201 tcgccccttt tggtgatact tcggtcataa aaactgctgc accaatagca aagggtgttg
    49261 cgataagggc tgagagaatg gtaacgataa aggaacccaa aatcatagga agggcaccaa
    49321 attctttact agaaggattc caagttcctc ccaaaagaaa gtcaaagata ttcacaccat
    49381 tgacaaagaa ggtcgacaag cctttttgcg ctacgaaaac caaaatcatg gccacaagga
    49441 tgactatcaa agaaagacag gcaaaggtca aaccttttcc taatttctcc agacgagaat
    49501 tctttgatgg aagcaacatt ttcttagcta attcttcttg attcattatt gtctcccttc
    49561 caacactgtc acagttccgg cagcatcttt ttcaaccttc atttccttaa tcggaatata
    49621 cttcaatcct ttgacaatcc cttcttgggt ctcatccgag agaacaaaat tgagaaattc
    49681 tgcagccaac tcattgggct gccccaatgt atacatatgc tcataagacc acaagggcca
    49741 attattgcta cttatatttt ctggacttaa gtcatagcca ttcaacttca tgcttttgac
    49801 cgaatcatct atataggtaa gagataaata agagatagct cctggacttt ttgatacgat
    49861 tgattttacc gctccatttg aatcctgctc ctgactttgc atggcagact gaccttccat
    49921 aatgacagta tcaaaggtag cacgagaacc agagccggct gcccgattga taacagagat
    49981 gggtaagtcc ttaccaccaa cctctttcca attggttacc tcacctatga agatttgacg
    50041 aagttgctct gtcgttaggt tatcaacatc aacctcctta ttgacaatca gagccaagcc
    50101 agctaccgcg accttgtggt caacaagagc agaagcatca attccgtctt tttcctcagc
    50161 aaatacatct gagtttccta tatcaactgc cccagactga acctgggaca agcctgtacc
    50221 agaacctccc ccttggacat tgaccgtttt tccaacatgg atcgtgccaa attcatctgc
    50281 cgctacttca accaagggtt gcaaggcagt tgagccaaca gccgttatgg attctccacg
    50341 atcaatccag ctagcacagc ctactaaaca agccgtcagc caaaaagcga taagagacag
    50401 agcaagcttt tttctttttt tcactgtttt tctcctcgaa aataattatg aatactgtga
    50461 attttttaag tagttcttta tgagttgacg catgaattct taccaaattt ctgcgcaatt
    50521 gattatttat ataatatagg ctatattact ctttcctaac ctcctttttt catatgtgga
    50581 taaaatctct tgtctatccc ttcccccatt gtcacccatt atagtcattt cgtgtctctt
    50641 tttccccttt ttaatgcaag ggaaattact ctccttagat gataatccaa aagctagaaa
    50701 ggtatctcaa acctctctac tctcccagac tagtttacaa ctaaaaggaa aagattctat
    50761 tttatgagaa atctagttta caagcggtaa gaacgctaat aactaaactt cttgtactct
    50821 ttgaaaatct cttcaaacca gtgttttgag ctatctatgg ctagcttcct agtttgctct
    50881 ttgattttca ttgagtagta aaactacatg taatggcaat caagatatca agaatcatcc
    50941 tactaaaaaa atccatactt tcactataac atagaataag atatttgact agcattttca
    51001 tttgaatctg aggccttttg gaaaataatt tttcaaaaca tttccagtaa cctttgcaaa
    51061 gcccaagcca ttgcctttaa ccaaaacttg gtaccaacca tttggcagac tttctgccag
    51121 ctgaacgatt tctccagccg catacttgac aaacgcttct tggccaattt caaccgactg
    51181 ttcgacctga ctcggtttca aggctaaacc aagagcgaaa ctgggctcaa agcgtttctt
    51241 cttaaaagtg cccagatgca gtccattgcg agcaatcttg agcttcccta aatctggcaa
    51301 aagttctggc aagagataaa gctggtctcc aaaagtctgc aagatacccg gtagattgac
    51361 cttcaaatgg ttttgggcaa attcctgcca caaggcaact tgttcacggc tgaggttact
    51421 cttacttgcc ttaaatttag gagctggatt gttaccctta aactgtagat gggcaacaaa
    51481 ctgaccctct cccttaaact gatgagggta catccgagcc gtttctggca ggtcaatacc
    51541 agctaccatt ccattgatat gctctactgg caacaagtca aaatcatact cttccagcaa
    51601 ccaattgaca atctcttcgt tttcctcggg tgcccaggta caggtcgaat aaaccagacg
    51661 accaccttca gctaacatgg tcactgcatc ctccagaatt tctctttgca agctagcaca
    51721 ttgactcgga taatctaagc tccaatagtc catagcatca ggttgcttac gaaacattcc
    51781 ttcaccagag caaggggcat caagaacgat taagtcaaaa tagcctttaa agaccttgac
    51841 caagcggtcg gcagattcat tggtcaccac gacatttgtc gctccaaaac gctccatgtt
    51901 ttcaaccaaa atcttagccc gtttgcttga aatttcattg gaaacaagta gcccctcccc
    51961 tgctagatag gctgccagtt gagttgattt gccccccggt gcagcagcca agtccaagac
    52021 cttcatacca ggactgggtt gagctacttg agccaccatt tgagcagcag gttcttgcga
    52081 ataaactaaa cctgtagcat gctcaggcga tttccctgaa accttcccat agtggcccca
    52141 aggggtttga gtaatggcat cagaaaagga aagttgctct tcttttaagg gattgacccg
    52201 aaaggccgaa accgcttcct cctcaaaaga ggcaagaaaa tctcttgcct catctcctag
    52261 tatctcttta tatttttcaa caaatccttc tggaaattgc atttaagttc ttttcctttc
    52321 gtaaatatag gactgaattt cctcctgcat ctcaagaggc accatcatga ccggctgtct
    52381 ggtttgaaaa tcaggagctt caccaaaaag ggtcacaacc cgatagccca gactttcccc
    52441 taaaatacta gctgcggcat aatcccatgg ttgcagataa gtgagatagg tcaacaaacg
    52501 ccctgacaaa atcttggcaa aactaatggc cgcacttcca tagacacgaa caccaagaac
    52561 cgctcggctc aaatcagcca gcccccattc attggtttcc agcataccac tattccctgc
    52621 aatgagaaaa tctccaagtg gtttagtttt aaaaggagct aggggcctat catttagaca
    52681 aactggaaat tccccaccac cgtggtaaca atcccctttg accacatcat aaatcagacc
    52741 aaactgtccc tgaccatttt caaaataagc catcataaca gcaaaatctt cctgctgggc
    52801 tacaaaatta ttggtaccat caatgggatc aatgacccaa accttgccct cttgaaccga
    52861 ggctcgcaga caaccttctt cagcacaaat cttatcctca ggataacggg acaaaatctc
    52921 accaaccaag agttcctgaa cttctttgtc cagtctggtc accaaatctg ttggagagga
    52981 cttggtttca acacgcaagt cttcctgcat atggtcaaga atgtactgac ctgctttctt
    53041 aacaagctct ttagcaaatt caaatttact ttccaagaga aatctttcct tccccttttt
    53101 ctttggcagc ctgaactgct cggtaaagtg aatagccact aaccgtttca aattctcgtc
    53161 ccaaacgttt ctcttcgctc ttgcttggca cgaccgcttt gaatccctta taggagtcca
    53221 gtaacttttt agcctctact ttgccttcat aggcagcttc aacatcatta aaaaaagaaa
    53281 gcactgaagc aagttcttca gtgctccacg acaaatctag tgggtaacta tactgtttgt
    53341 tcattaacta ataccagctc tcattcttgc ttcttttagt tcttgcttac gataactacg
    53401 agggagaaaa gcacgaatct catcttcatt aaaaccgatt tgcatacgct tggcatcaat
    53461 aataattgga cgacgcaaaa gactaggata ctgctcaatc aaatgaagca attccgatac
    53521 cgaaatactc tctacatcaa tattcaattt ttgaaaaatt tttgaacgag ttgaaatgat
    53581 gtcatcagta ccattttcgg tcaaggaaag gatgtgttgc aattcttttc ttgttaaagg
    53641 actggtcata atattgtgtt ccacaaaggg aaccttatgt ttttctaacc aggccttagc
    53701 cttacgacat gatgtacagc tcggtgatag aaatagtgta atcatgcttt tctcttctta
    53761 tctatacttt gctacttcta ttatacaaaa aaataaggcg cttgactagg gatttttaga
    53821 aaaaaagcct attttttcaa gaaaaatagg ctgtttgcga acgattgaca caattggatt
    53881 tggttaattc actcttaacg atggttttaa acgatatata tttttatata tgcaaattaa
    53941 aaacatcttt catttcactt cctacgactt ttcagacaca gatagccaaa gaagctttca
    54001 tagagggcaa aaaagaggag gaaggcatga agaaagaagg tctctggcaa aatcataata
    54061 acaggatcct tggctggatc aaaaagccag gtatcatctc ccacaaagag aatttgatgg
    54121 aaaagagtaa agaattggtc aaaaccaatc aaaactcccc caagtccaat catcacaggt
    54181 aagactacta gagccaggag actttttcga taaagagaca aaaagtcctt tttcacaatc
    54241 ctattgacaa agacatagaa acttggcagt gtcactagag ctactagctg aaccaaatga
    54301 aagagattct tgaccactgc gaaatggtgc agaccagctg ctgacgaacg aaaatcaggc
    54361 atctgtaaga cctgactaaa aggattggtc agataattca tcaagatatg aaaattgtat
    54421 tgaatggttt ctggttttag atagactcga ttcgttaagt ttagccactg aatctccata
    54481 ggatagaaaa tccaagccag ataaatggtc agaaggattg agagggagag gagaaagagc
    54541 atagagcccc aaaaggtcaa tttagttttc atcaaaatcc cactccgcaa ggctagaaac
    54601 cacatgtgtc ggtgcgattg gcaggccagc tacttcttct gccttggtaa aacctgtcgt
    54661 caccaagagc gttggaatgc cattgtcaat cccagcccgg atatcagtca aataattgtc
    54721 cccaaccatg attaactctt cacgttccaa acctaagtgc tcaaccgcct tgtccataat
    54781 gatggcattt ggttttccga tataaaccgg cttcactcgt gtcgctactt caagcagcgt
    54841 aatcagtgag ccagcacctg gcaaaagacc gcgttccgtc gggatgttga ggtcaggatt
    54901 ggttccgata aaatgggcac ccttttgaat agcaagagtt gctgtggcaa atttttcata
    54961 gtcgacttgc caatccagac caactaccac gtaggcaggt ttttccttgt cttccacata
    55021 accagccgcc ttgatggctt ccttgagtcc tgcttctccg acgacataga cggtcttttc
    55081 aagccccaaa tcattcatat agtcgatggt tgccaaagtc gctgtgtaga cagtcgatag
    55141 gggcgtatcg atattaaaat tctgagccaa catctcctta acactctctg gagtgcgggt
    55201 tgtattgttg gttacaaaga gatagggaat gtcccgcttt tgcaattcat gaacaaaagt
    55261 ctctccagca gggattcggt ctttcccctt ataaatggtt ccgtctaaat caattaaata
    55321 gcctttatat ttcatctatt tctccctaag ccttttttat ttcttgccaa gtaatgattg
    55381 cttgggcatt gataacccca tcacttgtaa tttcatgctt gctttccagt ccagtccgtt
    55441 caacagccga tgtaatcacc ccacctggtc gaacttcctt gacatacttg aggttgattt
    55501 tcttgggaat atagtgggtc aaaaaatccg ctcccatgac ctcaaaaatc cagtccaagt
    55561 atttactgtt attgacatga ccattcatat ccaagtcgta aaaacgaaca tggtaatcct
    55621 tgctgatcgg ttcttccaag gactcatact tcggtccacg gataagtttt ttatcaaaat
    55681 cagactggta aggagccaca atctcaggtt caacaacatg gacttttcga ctgtcgcggt
    55741 ccatgagaac aaaggtcgcc atcatgtgga tgagctcctg ctccgcttca ttataaatag
    55801 taaagcgacg gtagcaaaaa agtcgattgt agctcaaggc ttccgtttcg atggtaattt
    55861 cttccgcaaa acgaggcaaa cgaaccacct caatatcata ttctacgata atccagacca
    55921 gattatattc ttccaaaatg gccttatcac taactcccag ttcaatcgac tgcatccctg
    55981 aaacttgcag tgacagcaaa atcacatctg gaagtttgat atgaccgttc atatcagcca
    56041 tatcaaaagg aattttcatt ttcatttgat aagttaagcc catgatccta ctccaaaata
    56101 aatcgttctg ctacagtatc tcccaaaaag agacctctct ttgtcatgcg aacgtggtca
    56161 ccctcaatct gcatgaggcc ttgttgaacc aaatctctga caatttctcc ataaagtcca
    56221 gcaaaagact gtccaaattt ttcctcaaat cgcgccatgg aaaccccgga tttcttgcgg
    56281 agtcccaaga acatttcttc ttccatttgc tccttttgac tcaggtgatc ttctgtaata
    56341 caagcattgc cttcctcaac cgcactgaga taatgacgaa tgggaccatg atttttatag
    56401 cgtactccat tgacataacc agatgcccct gcaccaatac catagtattc agcattgtcc
    56461 cagtacatga gattgtgacg actttcaaaa ccgggtttgg agaaattaga aatctcataa
    56521 tgctcaaaac ccgctcgctc cagctctgca atgatgtact caaacatctc cgcttctagt
    56581 tcctccttag gcagaggcaa tttcccacgt cgcatccggt tcataaagac cgtatggttt
    56641 tctaaaatca aactatacaa actcatgtgg agaatatcca gtccaatggc tttagccaca
    56701 ttttccttta cttgctccat ggtctgacca ggcagagcat aaatcaaatc aatggagatg
    56761 ttgtcaaaac cagccagttt cagacgatcg atattttcat aaatatcctt ctccaaatga
    56821 ctgcgcccaa tctttttcaa catcttatca tcaaaggtct ggacacctag cgaaacacga
    56881 ttgacagccg aatttttcaa aacagctatc ttatccgcat ccaaatcgcc tggattggct
    56941 tcaatggtca actcttccaa gacagacaag tccaagtttt tagtcaagcc attcagtaac
    57001 acctccagtt gcggagccga cagggctgtc ggtgttccac caccgatata aagggttgac
    57061 aacttttcaa tatcataaga acgaaactct tccagcagat gctctaaata gctgtcgact
    57121 ggctgatttt tgatgaagac ctttgaaaaa tcacaataat aacaaatctg ggtacaaaat
    57181 gggatgtgca cataggctga cgttggtttt ttctgcatag taattattat accacaaaga
    57241 ctagattcca gataaaaatc accatcccca gatacatagt ccgtccggag atggtgatgg
    57301 tttattcttc tgttatatca atcacaatct cttctgagtc atcaagagct tcggcttttt
    57361 cttgccattg ttccttgaga ttatttaatt gattttttga tgcttctgtc gcttgaaaag
    57421 cataggattt agcttgagca agtatactgt ccacagtgat ttcacctgac tcaacctgtt
    57481 cttttgtttt cagaacaaaa tctgtagcct gctccttaac ttctgtcagt ttttcacaga
    57541 cttgctcctt ggcatactcc ggatcttctc tcaaatcatc tagaaaatct tgagcctgac
    57601 tgcaaacttg tttgccctta tcacttgtta aaaacaaggc aagagctgca cctgaaaccg
    57661 ttcctaaaag gattgaggat aatttaccca taaggattct ccttttttat tttttgaaaa
    57721 atttacttgc aagacgaaga gctgacagac ttgcaccagt cttgagtgtt tttgaaccag
    57781 ctgatgaagc tttcttgctc aagacacgcg catggtcatt gaggtctgaa acagatagag
    57841 ataaatctgc aacagcactg aagagtggat caatcgtagc caccttgaca ttgatatcat
    57901 ctgccaagac attgacctta gccaacaact cattggtgtg atgcaaggtc acatccacat
    57961 ctgaagtcaa ggttttaatc gtcttttctg tttcatcgat gacacgacca agcttttgta
    58021 cagtaatgat cagatagacc aaaaagacaa ttaaagctag ggcaacaaga atatatgcaa
    58081 cttctaacat ttagttttcc tcctctgtaa tatagtaagg ggccttcttt cgattttgat
    58141 aaataacgat cattataccg agaccgataa ggacaactga cagccattgg gacactcgaa
    58201 ggccgaagaa catgagacta tctgttcgca taccttcgat gaccatacga ccgaaaccat
    58261 accaaatcaa gtaaaaggcc gtgatatgac ctcgtctgag actcttccat ttccgtctaa
    58321 aaatcagaat caaggcaaag ccaagcagat tccatagaga ctcataaagg aaagtcggtt
    58381 gacggtagct cccctcaata tacatctggt cacggataaa gccaggtaga taatccagat
    58441 tatccactgt tgcaccataa gcttcttggt taaagaaatt accccaacgc cccaaacttt
    58501 gagcaatcat aacgctaggc gccgcaatat ctagaaaatc ccaagtattg atgagtttac
    58561 ggtcagcaaa gatatagagc acaagagccc cagttatcaa accaccgtaa atggccaaac
    58621 caccattcca aatggcaaaa atctctccta aattctgact atagtaatca aatcggaaaa
    58681 taacatagta gagacgagct cctaaaatag ccaagggaaa ggctactaag ataaaatcta
    58741 aaatatcgtc tggtatgatc ttctttctag gtgcttcttt catggtcaaa taaaccgcaa
    58801 gaatcaagcc tgtcacaata cataaggcat accaacgaat agctaggggt cctagttgaa
    58861 tagcaattgg atcaagcatt ttgcacctca tttcgagcga tgagacttgt cagtcgttcg
    58921 tcgaacaaac gggtcgcatc aaagcccatt tccttggcac gataattcat ggcagctgcc
    58981 tcaatcacaa cagagatatt acgacctgtt ttaactggaa tacgaatacg aggaatggct
    59041 acgccagaaa cttcaagttc ctctgcattg tttccaagac gatcaaaggt cttatgcgta
    59101 tcgtaatttt ccaaatagac agcaagctga acctgtgaag aatccttgac agcactcgca
    59161 ccgtagagac tcataacatc gataatacca accccacgaa tttcaatcaa gtgtttcaaa
    59221 atttcagctg gttcacccca gagagtaatc tcatccttgg caaagatatc gacacggtca
    59281 tcggctacca aacggtgacc acgtttgaca agctcaagac ctgtctcgct cttaccaatt
    59341 ccactatctc cctgaatcaa gacgcccatc ccataaatat ccatcaagac accgtgcaca
    59401 ctggtacgtt ctgccaaacg agaatccaga tagctagata actctccaga taaacgactg
    59461 gtagctgcac ggctggttaa aatagcaatc ttacattctc tagcagcctt taacatctcc
    59521 tctggaacca ccaaaccacg agcaacaatg actgctggtg tctcaggtag aaacattttt
    59581 ttcaaaactt cataacggct gttggaaggc atgctgatca gataagacca ctccttcatc
    59641 cccaaaagtt gaatccgctc tggtgtatag tagtcaaaat agcctgtcat ttcaagacca
    59701 ggtcgcgtaa tatccgctgt attgatttcc ttttcaagca actctggttc accatagaca
    59761 atgtctagtc taagcttttc aatcacttct tttactaaaa ccgacatcat ttcctccttc
    59821 aaatgagttc ttctctctat tatatcaaat tgtaggagga agtttcagat ttttgcaggc
    59881 tttggagtat ttatttaaaa gaaaaaggac tagatttctc tagcctttca tttcaattag
    59941 aattttttac gtttacgagc tacttctgat ttacgtttac gttttacaga aggtttttca
    60001 tagaattcac gtttgcgtgt ttcttgaaga gtaccagctt tagtaaccgc acgtttgaaa
    60061 cggcgaagtg cgtcgtcaag agattcattc ttacgtacta ctgttttaga catttttttc
    60121 actcccttca agttcaatat ctatttcatt ctacacgtaa aaggaataat tgtcaagcaa
    60181 aaatgcggtt taattttgat tttttcttcc atttatacaa gagttgaaag cgttcaatag
    60241 aacattgctt ttttattttt cgagtaagct aagcgcttca gcatctgcga tgatggttac
    60301 atcagggtga ttttgaaggc tacttgcagg taggttctca gtcactgggc cagatactgt
    60361 tccggcaatg gcttctgctt tcgactcacc gtaagcaaaa agaataatag acttggcatc
    60421 caaaatgttt ttaatcccca ttgaaatagc ttgggttggg acgtcttcaa tcttggcaaa
    60481 gaagcgtgca ttggcttcga tagtagactg gtcaagttct actagatgcg tttgactgtc
    60541 aaatggagta ccaggctcat taaagccgat atgcccattg cgaccgattc ccaagatttg
    60601 gaggtcaact ggatggtcag ccaaaatttg gttgtagcgt tcaacttcag cttcagcatt
    60661 gtccttaaca ccacgaggca agaaactttc tttaaatggt ttttggttga acaagttttc
    60721 ttgcatgaag taacgataag actgtggatt gtccccatca aggcccacat actcatcaag
    60781 gttgacactg gttagatttg aaaaatcaag gtcactctca acaatttcct tgtaaaattc
    60841 aagtgggcta cttcctgtcg caagtcctag agtttgagct ccattagcca atttttcctt
    60901 caaaatctca aaagcaactt ttccaccttg aacttggttt tcaactttaa taactttcat
    60961 cttttcatcc tccatttctg tctatattta ttatatggta tagaccaatt tttgtcaagt
    61021 gaaaacgatt taatttctaa aaaaagagcg tccaaacttg aaacgtgtta taatggatag
    61081 gattataact tagaaagaat gaacaaatat atgaatacag ctgattttga tttccacttg
    61141 ccagaggaat tgattgccca aacgcccctt gaaaaacgtg atgcctccaa actcctcatc
    61201 gtcaaccgtg agacaggaga aatgcaagat aaacatttcc actctattat tgatatgctg
    61261 gaacctggtg atgcccttgt catgaacgac acccgagttc tccctgcccg cctctatggt
    61321 caaaaagtgg agacaggagg tcatgtggaa cttctcctcc ttaagaacac tagtggagac
    61381 gagtgggaag ttctggctaa acctgccaaa cgcctcaagg tcggtactcg tatcagcttt
    61441 ggtgatggcc gcctcagcgc tgtcgttaca gaagaattga cccacggggg acgcattgtc
    61501 cgctttgaat accaaggaat tttcctagaa gtcttggaaa gtctgggaga aatgcctctg
    61561 ccaccttata tccacgaaaa attagatgac cgtgaacgtt atcaaaccgt ctacgccaag
    61621 gaaagcggct ctgctgcagc accgactgct ggtcttcact tcaccaaaga actgctggca
    61681 gaaatccaag ctaagggtgt tcatctagtc tatctgactc tccatgtcgg actcggaacc
    61741 tttagacctg tttctgtgga taatctggac gaacacgaaa tgcactcaga gttctatcaa
    61801 ctttctgagg aagctgctgc cacccttcgc tctgtcaaag aaaatggtgg tcgcgtcatc
    61861 gctgtcggaa ccacttctat ccgcaccttg gaaactattg gttccaagtt tgatgggcaa
    61921 atccaagcag attctggttg gaccaatatc tttatcaaac ctgggtatga gtggaaggtc
    61981 gtggatgcct tctcaaccaa cttccatctg ccaaaatcaa ctctggtcat gttggtttct
    62041 gcctttgcag gccgtgaatt agtcttagat gcctaccacc atgccatcca agaacactac
    62101 cgcttcttca gttttggtga cgccatgttt atttattgag aaagaatttc tctaattctt
    62161 ctaataccaa taaatcgcta agatattatt tcaaagaaca tctacaattg aaactctagc
    62221 tagctgtaga agacgcctag tacattgaaa ttaaaatgct tccccctagc ttcgaaaata
    62281 ttgccataga ttgcgttgac tctccaaatt gattcatcta tattttattt cagcttccta
    62341 tattttcttc gctgtttgta aatcaaaatg caagacacat gagtagcacc atatttgtta
    62401 ctcttatctg tcctctcaag agactattat gagttatttc agaatcattc actactttga
    62461 ccctgactct ccttagtctc aaaatcaaag acttatactc ttcaaaaatc tcttcaaacc
    62521 gcgtcaacgt caccttggat tatatatgtg actgacttcg tcagttctat ctacaacctc
    62581 aaagcagtac tttgagcaac ctgcgactag ttttctagtt tgctctttga ttttcattga
    62641 gtattaaaca aaaagtgaac aaatctgaat tctaatgtac agaagactag gcttgttcac
    62701 ttttttatag tcgctataag atgaccttat ctatagcttt ttatatataa ttatatattc
    62761 agacatacta ttatcaattt tgtcgcaggg aggaatctgt taacgcaccc attcaccatt
    62821 atcattgact ctatagccat ctatacttgt attgaccgct aactcacccg atgcatttac
    62881 ataataccat ttaccaccaa cttggaacca ttgattgact ttcatagaac cgttgctgtt
    62941 gaggtagtac catgaaccat taacttgtac ccaacctgtt gccatggaac catcagtatt
    63001 ataaaaatac cacataccat tttcttgttt ccagtctgtt gttggagcaa ctgctttagc
    63061 tggttctact gctacatctg ttccttggtt agatgtaaca gatacaggat acgaaggaat
    63121 agatgattgc tcaggaacaa caactttttc aggttctctc gtccctctcc ttatacgtct
    63181 ttttaccatc tctttagtaa tttgacgaga agtagtttct tcaattgttc catcacgttc
    63241 atctacagta tagattgtag taagagtaat ttaccaattt ctcctacttc ttctacttct
    63301 tgacttttat caagagttgg gccatcgaga tattctgttt cgattggaat ttcttggaca
    63361 agaacttggg gcttggttct ttttttaaca actcttgttt gagagtcttt ttttgactta
    63421 aagtactctc agttacttgt ccactctttc catctacatt ataagttatc gttgtaactg
    63481 ttttcccatt ctttcctaga gtaatctctt gctcctgtcc tgcagaaagg tcattgtctg
    63541 cttcatattt agtagcaaat ggaacaagaa cttcttcaac cttgctttta gctggaactt
    63601 tgataactgt atccgtggct tcttttctat caacagtaac ctgttcggta acataaccag
    63661 tctctggatt aacatcgtag gtccttgtcg tagttacata gccatcctct ccatcaattg
    63721 taacaggatt ttcactacgg tcttttgttt catctttttc ataacgaatt cgcgtacttg
    63781 aaattttctt ggttactacc ttaggtttag tcgctacttt tacaataata tccccattgt
    63841 cagcgtcatc atactctatt ccctcttctt tatctctagt atcatctctg acatattgaa
    63901 tcccatcagc agcatgaaca aaacttgtat tcagattcct cctaaaaata aagttagccc
    63961 gattaccgca gaaccaaaaa tctttccgag tttacgtatt gcatagcgct tattagtatt
    64021 agattttgcc attacatcct acttctagta tagcatcttt tctatcaaac gttaaacaat
    64081 atactttata tataaaatag acttagaatg atatattgat tattgaacta acactttaac
    64141 tatatcgtaa tcaatctcat atataaagga ttgcagacat cttatctaaa tacatgctaa
    64201 tatatttaga tacaaacatt ccaacttgat aattttcact catctttcat cattccttat
    64261 acaactatgc agtataaata gaatagtttt ctcatcagaa tgagactatt ttaatattag
    64321 ataatttgat agaaaaaaca tgaataaaat tggttgaatt taattcccta actcgataga
    64381 ggattctatt ttaaatttaa caacagctag gattacacta taaaaagtac attaccacct
    64441 gttcggctgg agaatctagt cgattagctc cctcttagtt acaagcttca agcgattttt
    64501 taactaacac taaaccagat taaagaagga actagaacat ctgtcttgta ctaatcaata
    64561 tgctatttta aagtttcaaa taaacttgga atgctaatta ttatatttac aataaatagt
    64621 tgacttcgat agaaacagaa aatagaattc aacattcttc cctacttata ccataataaa
    64681 taagatccgc aagatagcct tttctctcta caccattaac aatggtttgt agataggaca
    64741 ttccagcctt ttccatgaca cgacctgaag ctgggttgag actggcataa cgtgctctga
    64801 ccttttgaaa acctgcttga gtaaaacaaa agtccaagat agctttcaaa gtctctgtca
    64861 tcataccatt tccccagtaa gccttgccta acacatagcc aatttcacag cttaaatcag
    64921 cctcgtctat cttaacaatg ctgatatctc ctattacttg ctctgggttt tcttttagac
    64981 aaatggccca tttataatag ttgagattag tataggaagc aacccaattg caaatcgagt
    65041 ttcgagtgat ttcgacatca ggatggggat cccaggtaac ataggtcaga ttctcagcgg
    65101 atgaagccca attttgaaac atggcttctg catcactctc cacaaatctt cttaaaatca
    65161 aacgttctgt ctgtaatatt tgcgtaccga tagatttcat gatgctacct cacttcctta
    65221 tttctatact cgtagtaaaa aggctcaact cagtaacagc tagaaaacga gtttgtcttc
    65281 taatttcttg acgcagttgc tgcctggttt gaaatgcgct ctagcaagct ttttcaaacc
    65341 tagtcatcgt tgcaggggta agacgatgaa atcgaactct tcgagttact gaatctgcct
    65401 cattttctta tttctaaact cggggttcaa gatcccccag accttctctc actttcttat
    65461 ttcaagatga gaggctgaaa acctccagtg gaggtttttc ttgcttttta atttcagggc
    65521 aagaagctaa aaaggtcccc cggacctttt tactcccaaa agtcatcaaa tacggtgatg
    65581 ggtaagtggc gtttgtgttg gcctttgtgc caccagtttt caatggtcgc ttgagcttct
    65641 gggctgattg ttttgccttc taggtagtcg tcaatctctg cgtaggtgac tccaagtgcg
    65701 acttcgtcag ctaggcctgg tttatcttct tctaggtctg ccgttgggat tttttcataa
    65761 agggctggct ctgcgccaag tttctgcaag agctgttttc cttggcgttt attgaggcgg
    65821 taaagaggga gaatatccgc accgccgtca ccaaacttgg taaagaaacc tgtgatattt
    65881 tccgcggcgt ggtctgttcc aatgaccgct ccgctatggg aaccagcaag ggcatactga
    65941 gcaatcatac ggcaacgtgc cttgatattc cccttgttga agtctgaaac aggactacct
    66001 gtcgcttcaa ctgcagctgt catggcatca gctgattcct tgatattcac aaccaagctg
    66061 acatctggct ggatgaaggc tagggctttt tgagcatctg cttcatcagc ttgcactccg
    66121 tatggcaggc ggacagcgat aaatttgtag ctatcgtctc ccgtttcagc tcgcagttct
    66181 tccatagcta attgcgccaa acgtcctgcc aaggttgagt cttgtccccc agaaatccct
    66241 agtacaaagg tttttaggaa gggatgtttt ttcagatatc tttttaagaa atcaatagaa
    66301 cgacggattt cttcctgggc atcaatcact ggtttgacac ccagctcttg gataatcgtt
    66361 tcttgcaaac tcattcttct tctccttcac caagggcttc cttgcgcatc ttatcaatca
    66421 agtccatctt atcttgccat acatcacgcg ccaaatccac tggatagtgc tgcggattga
    66481 gcacacgctt atactcatcc cacaacttgt caaattcctt acgggcataa tcctgaatgt
    66541 cagtcaaact aggcaagttg taaactaata ttccttcttt gaagatatcc accaagagag
    66601 gaacggcatc aaaattacga accgtcttct tgatgtatgt ataggtcgga tggaacatct
    66661 tgatttctgt catgtcgcta atatccacac catcataagt gatatagtcg ccttctgact
    66721 tgcctttttc acgactggta atgcgccaca cctgcttctt acctggcgta gaaacttttt
    66781 cagcattatt agacagctta atcgtattgc gcatctgacc agtttcatct tcgattgcaa
    66841 caatcttgta aaccgcccca agagccggct ggtcataggc tgtaatcagc ttggtaccca
    66901 caccccagac atcaatcttg gccttttgca tcttgaggtt aaggatggta ttttcatcta
    66961 gatcattaga agcataaatc ttagcctctg taaatccagc ctcatccagt tgctgacgga
    67021 ctttcttaga aatgtaggca atatccccag agtcaatccg cacacccata aagttaatct
    67081 gatcacccag ctcacgcgcc acctgaatgg cagctggtac accgatgcga agggtgtcat
    67141 aggtatccac aagaaagaca caatttttgt gggtcgcagc gtaagccttg aaagcttcat
    67201 agtcattgcc ataaacctgt accaaggcat gggcatgggt tcccaaaaca ggaatgtcaa
    67261 agagcttacc cgcacgcacg ttgctagttc cattggcgcc accaatcaca gctgcgcgtg
    67321 ttccccagat ggccgcatcc atttcttgag cccgacgtgt cccaaactcc atcaagggtt
    67381 catcttcgat aaccgaacga atacgagctg ccttcgtcgc caccaaggtc tggtagttga
    67441 cgatgttcaa aagagccgtt tcgaccaact gacattgggc tagaggtcct tccacctgca
    67501 caatcggttc attagcaaaa accaaatccc cttcttgggc agaacgaacg gtcaactcca
    67561 acttgaaatt gcgaaggtaa tccaagaacg ccccatgata accaagcgac tccaaatagg
    67621 ctatatcact atctgaaaaa cgcaagtctt caagatagtt cacaattctt tccaaacctg
    67681 caaaaaccgc atagccgttc ttaaaaggct gttggcggaa atacacctca aagaccgcct
    67741 tcttattgtg aatcccttgg tcaaagtaaa cctgcatcat gttgatctgg tacaagtccg
    67801 tgtgcaatgt caaactatca tctggataca tacttttcct acttccttag ctagaaaccc
    67861 atgaaaattt tcaagaactt tcatgtattc caataaatta gtactattat atcacatttt
    67921 agctggattg agaaaagagt aacaagctat tctccactct ccaattcatc catatcttgt
    67981 tcaaattttt tctgagccca ttcgccatag ctcttaagac caagattgcc aataaagatc
    68041 cacggaaggt aaatgatata agtaatgacc caagcagaca ggtatttaaa attcaaagga
    68101 ttgtgctgat aaatttctat gttgaattga taattctgca acatcaaaag agccgtaata
    68161 gccaaggtta ggaaaaaaca acccaaaatc gtaaaatgaa aacgactata gtaggtcact
    68221 cccagataac gggcacgatt gaaaaagtaa aatgtcccta tgatgataac gattagcagc
    68281 atattagaat taaaaaggct tggtgctaat actgaaatga tataagatag gagcgacaaa
    68341 gcgatgcaga tatagaaact ttcagagccc gctttattga acagttgttc ttctctttcg
    68401 tctagtaatt gataataata aaatctattt ttcatcttct tcctcccaaa atagttggtc
    68461 tagggttttc cctaaacatc tgcaaatgga ctggcagagc gagagactgg gattgtattt
    68521 ccccgcttct atcaaaccaa tagtctggcg cgtcaccccg acagcctctg ccagttgacc
    68581 ttgtgttaaa tcacgctcta cccgagctaa ttttaatttt aaatttttag ccaccttcgt
    68641 cctccttata gttttaatac tcatctactc ttaaaaaatc caaaaccaac acaagctatc
    68701 agctatttat tataacccca ttaatttgca ctccagtatt atcttcagcc gaaaatcagt
    68761 attttaggtt gtttgaaata aacttcctag tttactcttt tatttttatt tagtattagc
    68821 tttagtagga tcttttaaac gagtttttat tgcttatatt tcttgtttag tttagttggt
    68881 ttcatatcac tattatataa tgcttttgga aatgagccta gtataagtca aaaacgtgtt
    68941 gaatagtgaa agcaggagca taccaagatg actgattttt ttattgtatt ttaggtatgc
    69001 aaaataatac taaatgaaac taaaatgaat acttcttgct tttcttgaga gtgaaaaagt
    69061 acaataaaat gattgtagtg attaccacaa gatactgact aatagacatc gtatttgaag
    69121 ccacaaatac aggtaaagaa taaaaaggaa gaagttcaat tgctttgata aacaaatcat
    69181 tactagtatt catcatcatc gtatttaata tattagggat aaagagtagt aaagctacta
    69241 aaataccaaa taccaagcca cttttcaatt tgtataaata aaaaatgtaa gataatagaa
    69301 aataaaacag gactaacaaa attgtattga ctacaatagc tactataaaa tctaatccta
    69361 aatcacctgg aatacccaat ttacttacta tgactagtaa cacaatgcca ttcaaagcat
    69421 aaataatgga aactgagatg gcatatagaa aattagacaa gacgtaaatc atccgattct
    69481 tctgtttatt taatttcatg attataacct tcctttattt tgaacgattt aggtaaatat
    69541 ctttaagcgt ctctttttta gtttcaaaat caactacttt aatagagttt tcgttaaaaa
    69601 atttaaaaag ctcactattt tgaatattcc cagacatagt aattctcaat ccctcttcct
    69661 gaacaatatc cccaaattct tgtttggtaa tgaaaatgtc tctatctgta gctgatgaaa
    69721 aagctatctc aaacaagaaa ttatgactat cttttcctac tttttgaaat gtcaaaagcc
    69781 cgctctccaa gaaaagaacc ctctcacaaa tttcttcaat gtcttctaat ttatgactcg
    69841 atattaaaat tcccacgttt tcatttaaag ctaatttttt ttagaaccgc taaaactatt
    69901 tgtgatgatt caatatctaa accattagtc ggttcatcta aaatcaatat atcaggttcc
    69961 gtaacgagag ttagaagcaa agctaatttt tgttttgtac ccaaagaata ggtctttact
    70021 tttttattaa tagactgagt caaatctaac tcttggatca aacttctaaa tctttcttga
    70081 ttgtagtcaa caccatataa atttgacaaa tattttaaat tctctaaacc tgttttagat
    70141 aaaaataatt ttggttcttc gattaaatac ccaacattat cactgtttat aatattacct
    70201 gaagtcggtt gattattttg aacaagaatt ttcatcaacg tactcttacc aactccattt
    70261 ctccctacta gaccaacaat ttcacccttg ttaagtgcaa aatttatatc tttgagaatg
    70321 attgaatgac cataatgctt acttacattt ataacttaca ttttttattc ctccattaat
    70381 attcatggtt tgctctttga ttcttattga gtttaaaaag ttgcgcctct ttttaacaat
    70441 attatatcat atatctaact taatgcaata tataaatgtc attttgtaaa attttattaa
    70501 caaaaaactc tctacaacaa aataatagag agttttctgc ttattaattt ttttcaaaat
    70561 atctaagaca tcgcttcttt gtttaatttg ataggcttta atacctaagc tccgacttta
    70621 tcacttaaaa aataaaaaag gacagctaat ctcttgcgca tcaagggatt tcgccattct
    70681 ttaatctcgt ttaagtgttg tatatggagc tttgcataat tttttaaaac taaacaacta
    70741 cattaagcac gaactgtgac gctggttcca tcttccttat aaagattgat aagcccctct
    70801 ttaagagctc gaaccatgtc tccagcttga acaagttgtg ggactttaat agtcaatagt
    70861 tccattggat ttggagcgcg gtcgatttta ttgcctttgg catcatgcaa atcttcaata
    70921 taggtttcaa aatgacggaa acctggacca taaaactcaa cttggtcccc ttcgttaatg
    70981 acgttccgtt gacgaatggt tgctgtttgt gtcgcatcat cataagaaac cacttcagcg
    71041 acaaacttgt actcagggat tttacgacga gcaccaaaca actgctcatt ttcagatggt
    71101 gtaccatagt aaaatcctgt agccagttca cgttgggcaa ccttccacat ctcgtccacc
    71161 aagtcttgtt tgatagcttc aaacttttca ggactttcaa gataggcatc cacagccgcc
    71221 ttgtagcagt tggttactgt tgatacatag tgaatagact ccatacgtcc ttcgattttt
    71281 agactgtcca caccattttc aatcatatct gagatatggt caatcataga catatcaacg
    71341 gctgacattg aaaattcttc tggaatctca ccctgcaaac tcttacgttc tttcccaaat
    71401 ggcatatcgt aaaggtcgta cttccaacgg catgactgag aacatccacc acggttggca
    71461 tcacgcatac tctagacgga ttgttttgac aattctaacc ttttagactt tgcataataa
    71521 ctgttaaaca gtcgaaaatt accccagttt taacaagtag tcaaaatacc agataaagta
    71581 aaaaggggct aggaaaaaag tctttaaaat cttaaaaacg catagtatca ggtattgaac
    71641 aatcttgata ttatgcgttt tattgtggga agatttactt cattttctct tggaattgag
    71701 ttttctccta cttcaaaact agatgatttg ctatttaaaa tagcgttcct tatgattttg
    71761 gcaacctgga ttaaagcgag aaaaacaaaa ggggcaactt aagctttctt tatattgctt
    71821 atatgttagt agttttagac aaacaccaca taaaataggc ttatcctgta tcaaagataa
    71881 gggatacggc tcaaaagggt gattttctaa tgaatcatga caccgataac aagcataata
    71941 ctttttacaa tcatagcatt gtaaagcaat aatatccagc ttgctatggt aatgaatgca
    72001 tcttgtttgc ttatccacta agaaaccctg tgcctctatc atttcctttt cctttcttaa
    72061 gctcgaactg tgacgctggt tccatcttcc ttataaagat tgatgagtcc ttcttttaat
    72121 gcacgaacca tgtctcctga ttgaacgggt tgaggcacct taatagtcaa tagttccatt
    72181 ggatttggag cgcggtcgat tttattgcct ttggcatcat gcaaatcttc aatataggtt
    72241 tcaaaatgac ggaaacctgg accataaaac tcaacttggt ccccttcgtt aatgacgttt
    72301 cgttgacgaa tagttgctgt ttgtgccgca tcatcataag aaaccacttc agcgacaaac
    72361 ttgtactcag gaattttacg gcgagcacca aacaactgct cattttcaga tggtataccg
    72421 tagtagaaac ctgttgccaa ttcacgttgg gcaaccttcc acatctcgtc caccaagtct
    72481 tgtttgatag cttcaaactt ttcagaactt tcaagatagg catccacagc cgccttgtag
    72541 cagttggtta ctgttgatac gtagtgaata gacttcatcc gtccttcgat ttttagacta
    72601 tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatgtc aacggctgac
    72661 attgaaaatt cttctggaat ctcaccctgc aaactcttac gttctttccc aaatggcata
    72721 tcgtaaaggt cgtatttcca acggcatgac tgagaacatc caccacggtt ggcatcacgc
    72781 atactcatgt ggtttgaaag agtacaacgt ccagagtatg aaatacacat agctccatgg
    72841 acaaaggctt caatttcaac atctgtacgt ttgcggattt cagctaattc ttccattgaa
    72901 acctcacgcg ctaaaacgac acgagtcaag cctagctctt tccagaactc aagggtttca
    72961 tagttagtgg cactggcttg ggtagaaagg tggatttcaa ggcctggtgc ttcagtcgct
    73021 gcaatcataa tcaaggctgg gtcagatacg ataactgctg caatcccgat atcacgcagt
    73081 ttacggaacc actcaccagc accagcttca tttccttcgt gcataaccat attagccgct
    73141 acatagacct tggcaccata cttggccgca aactgcacgc cttcttccat ctgttcgaaa
    73201 gtaaagtttc ccgcacggct acgaagacca taggcctgac caccgataaa gacagcatct
    73261 gctccatact gaacagctac ctttagcttc tctaaagtcc ctgcaggtga taaaacctca
    73321 ggacgtttta atgtttttgt catgatttct cctatttgca atataaaagt ttgacatttt
    73381 tccttctcat tttatagtaa ataaaggata aaatcaagcc tagacggatt gttttgacaa
    73441 ttctaatctt ttagactttg cataataact gttaaacagt cgaaaattat cccagtttta
    73501 acaagtagtc aaaaagtcta gaaaagtaaa aaccagtatc caaagatact gactcgtaaa
    73561 aatacataaa ttagtcctta gatgactact cccactctgt tgctcctata atggaaaagg
    73621 attgatacag tctagaatat cgctttttta tttattattc tgatataaaa ctgttagaat
    73681 tgattttttg ttttataccc aaatatgaat agagacgtag ctaataagtt gaataacata
    73741 tttggaaaaa tgagaaatag caatattact agaactttta gaaacaatca atatttaaaa
    73801 agaaaaaagc tcaaagaatt tattaaacca tgttataata gatatagcta taattttatt
    73861 tataacaaga ggatagaaat gaccgaatta gaaagaaaaa atcgaaaaat tagctaagaa
    73921 atattctgat aacttaaaca tcaaagttca agagagagtt cgtgaaatgg caaatgataa
    73981 taagagccat tatttgatat acagagtttt aggtatttca tttgaagaag gagaaaatat
    74041 cgatttgtat caaaataaag gtcgtttttt atacaaatat gctggttcat ttttagaaga
    74101 agctgcagta ctatgcttta acgaaaaatt tggtacagaa aatacttaaa aagttaacat
    74161 tcctaattct gaaagtacaa aacctaagac ttttgaaatt gattgtttag tcggagaaaa
    74221 acacgcatac gaaataaaat ggtgggatgc aactacagat ggagaccata taactaaaga
    74281 acacactaga ataaaagtta ttcataacaa aggatatata ccaattcggt taatgttcta
    74341 ctatccaaat agaactcaag ctataaaaat tcagcaaact ttagaaacat tgtataacgg
    74401 tattggaggg aaatattatt atggagattc tgcctgggaa catttaagag cagtgaccgg
    74461 tattgattta cttagtattc taacagatat tgcaaataaa aaaacagggg taaaatcaaa
    74521 atgacagtat taaaaggaga taacttagaa atattaaaaa ctattgaatc ctcaagtatt
    74581 gatttaatct atatggaccc tcctttcttt acacagaaaa cccaaaaatt atctaataac
    74641 aaaaatatta tgtattcatt cgaagatacg tggacttcga ttgaggatta caaagaattt
    74701 ttgtctgtaa gattagaaga atgcaaaaga gtgctaaaaa atagtggcag tattttcgtt
    74761 cattgtgata aaattgcaaa tcatcatatt agattaattt tagataatat ctttggagca
    74821 gatatgtttc aaagcgaaat tatatggaac tataaacggt ggtctaattc aaaaaaggga
    74881 ttattgaaca atcatcaaaa catttacttt tattcaaagt caaaagattt taaatttaat
    74941 acaattttta cagagtattc ttctactaca aatatcgacc aaatactagt ggaacgaaaa
    75001 cgagatggaa actctaaaac tatatataaa gttgataata atggtaacta tattctagca
    75061 aaagagaaaa atggagttcc cctttcagat gtttggaata taccatttct taatccaaaa
    75121 gctaaagaaa gagtaggtta tcctacacaa aaacctattc tgttattaga acaaattata
    75181 aagattgcta ctgataaaaa tgatatagtt ttagacccgt tctgtggaag tggaactact
    75241 ttagtagcct ccaagatttt gaatagaaat tatatgggga ttgatttatc tgaggaagct
    75301 atcaatataa ctcagcaacg tctggaaaat gttataaaaa caagttcaga tttattgaat
    75361 aaaggaatcg aagcatatag aaccaaaact gaggaagagg aaaacattct taaattatta
    75421 caggcaaaaa ttgttcaaag aaataaagga attgatggtt ttttacctaa acattttcaa
    75481 aaaaaaccga tacctataaa aattcaaaaa aataatgaat gtctgaatga gagtatctct
    75541 ttattacaga atgctataaa ctccaaaaaa cttgattttg gagtagttat aaaaactcat
    75601 tcagataatt tattatttga ttttgatacg attcctgaaa atataatagt agttgaccat
    75661 tttgaactta caatcgaaaa atggttaagt aaaagttaac agttactata gtttgcaaat
    75721 tatttacaga aagaacaaaa atgctctgaa acactgattt caaagcattt tttgttagtt
    75781 taaaattatt cccactcttc ttttccaaac gtacaatata tccaaaacca ttcaaaatac
    75841 tagattctat tttttataat atcactaaat ccacctaatt ataggacgtt ttcagatttt
    75901 tagtcccgtc ccagtcccag taccggagaa atattgtttt aatataatat ctctttttgt
    75961 cttctaagct cttaaaagca aaagaacaag taaagagtca agacaaggat aaaaagtcca
    76021 tattagggca aataaaaagc tttaagacag atgacaaatc taagtcaaat aagaaagacc
    76081 atagcaaagg tgcagagaga taaatattgg cggtcttcgg actgccttta tttttttatc
    76141 catttttcaa atcaaattta ttcagactat atatgcacat atacacttaa attcatataa
    76201 aaacatggct tgtaaaaaat tactttaatc acaataatcg catttaaaat tgtgatgttt
    76261 gcaagctaaa ttacggactt cacttggaag tttccccttg tatcttttat aatagataga
    76321 aaatttgctg gcagatgaat atccaacaga ttctgctatc tcttttatag gtagttcagt
    76381 gtttaaaaga agagtttcag ctacattcat tctttttctt tgagtgtact ctgtaatgct
    76441 ttgacaatat ttttccttaa ataaattttt taatttagta ccactcattt tagatatttt
    76501 ttcaagcgtg ccttgattta cattcgttgc aaaatgatca tctaagaatc ttgctacatc
    76561 ttcaagtgct ttatcatcat caatttcaat tttatatttt tttctattta agtatgtgtc
    76621 aattactata cttatccatt catttgcctt tgctttaaag aaaaaatcag cggcaggagc
    76681 gtccatctta caatttaata tttccattgc cactctttct aaggcctttg taagtattat
    76741 ttgattcggt tgaagcaagg ttgaataaaa agattctgga ttaatgttaa tagatgctaa
    76801 atgtttttct attagctctt ttttaaaacc aatggaaaca gcaagataac aacaattctc
    76861 gtgtaataaa aaaacaaaat catcttttat attatcaaaa tcaaaagtac ataaagagtt
    76921 tgcggtaata gtttgatacg gattaaactt ttctccgttt gcactgacaa tgtaacttga
    76981 ataaattgaa acatagtctg acatactata agtgctattt tgaactactt cctctttgat
    77041 ataaaaatca tgtatatcga taatgaagct gcctccttca taaaaccaat ataggccttc
    77101 tgcataagtt gaattctctt ttttccaaca aaatgtatga cctgcacttg aatacttctc
    77161 attgttttta cattcatcta ctttcatata atcttttaca aaatcataat atgacataac
    77221 acactatccc ttttagacaa tattccaatt agccttatta attcaaaact attgtattag
    77281 taattataac agatgtataa tagaaaagca atgatagata ttatcaatta agcgaattta
    77341 tatctaaaag ggatattaaa gaaaggagat atgcttatga agatttacaa aaaactattt
    77401 gcttatgtcc aagataagaa atatcttggg gttttggcca taattttttc tgctatatct
    77461 gctctactta cagtatatgg atattattta atctacaaat ttctagataa gttaataatt
    77521 aattcaaact tatccggtgc agagagtata gcattaaaat ctgttattac actaacaagt
    77581 ggagcgatat tttattttgt ctcaggaatg ttttcacata tcttgggatt taggcttgaa
    77641 acaaatttaa gaaaaagggg aatcgatggt ctggaaaaag caagttttag gttctttgac
    77701 ttaaatccat ctggtcaaat aagaaagatc atagatgata atgcagcaca aactcatcag
    77761 gtggtagcac acatgattcc cgatagttct caggcaataa tcacacccgt acttgtactt
    77821 gcacttggct ttatagtaag tataagggtt ggcataattt tgcttgctct tactataatt
    77881 ggtggcttaa ttttaggggc aatgatgggc gagcaagaat ttatgaagat ataccaagaa
    77941 tccctatcta aactaagtgc tgaaactgtt gagtacgtga gaggaatgca agttgtaaaa
    78001 atatttaaag caaatgtaga gtcttttaaa agcttttata aggcgataaa agattactca
    78061 aagtatgctt atgattattc cctatcttgt aaaaggcctt atgttttgta tcaatggtta
    78121 ttttttggac tgattgcaat tttaattatt cctatagttt attttatgac tagcttagct
    78181 agcgcaaagg tgattttact tgagcttatc atgattttat ttttatcagg agttctcttt
    78241 gtttcattca tgagaatgat gtggtactcc atgtatattt ctcaaggaaa ttatgcagta
    78301 gatactttag aggcgcttta cgaagatatg caaaaagaca aattagtgca tggtaatgtc
    78361 aataatttta aaaactataa tatagaattt gagaatgtta gctttgctta taatgataaa
    78421 gctgtcattg aaaatttatc ctttaattta gaagaaggaa agtcctacgc acttgtcggt
    78481 tcatctggat caggcaaatc aacagtagca aaacttatat caggttttta caatgttaat
    78541 aaaggaagca taaagatagg cgggatagca ataagtgaat attctgacga agccttaatt
    78601 aaagccattt cctttgtttt tcaagattca aaattattca agaagagcat ttatgataat
    78661 gtagctttag ctaataaaga tgcgacgaaa gatgacgtta tgagagcctt aaaattagca
    78721 ggatgcgatt taatattaga caaattccca gaaagagaaa atacaatcat aggctcaaaa
    78781 ggtgtttatt tatccggtgg agaaaaacaa agaattgcaa ttgctagagc aattttaaag
    78841 gattccaaaa ttattattat ggatgaagca tcagcatcta ttgacccaga taacgagttt
    78901 gaattgcaaa aagcttttaa aaatcttatg aaggataaaa cagttatcat gattgcacac
    78961 aggctatcta caattaaaga ccttgatgaa attattgtca tggatagtgg aaaaattata
    79021 gaaagagggt ctgacaaaga attaatgtca aaagatacaa ggtataagag cctgcaagag
    79081 atgtttaaca gtgcgaatga atggagggtt tcaaatgaaa gagttttata aaaaaagatt
    79141 tgctcttaca gatggaggag caagaaattt aagtaaagca acactggctt catttttcgt
    79201 ttattgtata aacatgcttc ctgccatatt acttatgatt tttgctcagg aagttttgga
    79261 aaatatgggc aaaagcaatg gcttttatat agtattctca gttttgactt tgatagcaat
    79321 gtatattttg ctttctatcg aatacgataa attatataac acaacctatc aagaaagtgc
    79381 agatttaaga ataaggacag cggagaattt atcaaaatta cctctatctt acttttctaa
    79441 acatgacatt tccgacattt cacaaacaat catggctgat attgaaggca tagagcatgc
    79501 aatgagccac tcaataccaa aggtgggcgg catggtactg tttttcccat taatatctgt
    79561 aatgatgcta gcgggcaatg tcaagatggg tttagctgta attattccat ctattttaag
    79621 ctttatattt atacctttat ctaaaaaata tcaggttaat ggacagaata gatattatga
    79681 tgtcttaaga aaaaactcag aaagctttca agaaaatatc gaaatgcaaa tggagattaa
    79741 agcatataat ttatcgaagg atattaaaga tgacttatat aaaaaaatgg aagatagtga
    79801 gagagtacac ttaaaggcag aagtaactac aattttaact ttgtctatat cttcaatatt
    79861 tagctttata tctcttgctg ttgtgatatt tgtcggcgta aatctaatta ttaataaaga
    79921 gataaattct ctctacctta taggatattt actagctgct atgaagataa aagactcttt
    79981 agatgcatct aaagagggct tgatggaaat attttattta tcgcccaaaa tagaaagatt
    80041 aaaagaaatt caaaatcaag atttacaaga aggcgatgac tatagcttaa aaaaatttga
    80101 tattgatcta aaagatgttg agtttgccta caataaagac gcaaaagttt taaatggtgt
    80161 aagttttaaa gctaagcagg gagaggtcac tgctttggta ggtgcaagtg gctgcggtaa
    80221 aacaactatc ttgaaactta tatcaagact ttatgattat gacaagggac aaatcttaat
    80281 cgatggcaaa gatataaaag aaatatcaac agaatctctt tttgataagg tctctatagt
    80341 tttccaagat gtggttctct ttaatcaaag cattatggaa aatattagaa tcggaaagca
    80401 agatgcaagt gacgaagagg ttaaaagagc agcaaaactt gcaaattgca cagattttat
    80461 agaaaaaatg gataaaggtt tcgatacagt tattggtgag aacggagctg agctatcagg
    80521 aggagaaaga caaagattat caatagccag agccttctta aaagatgcgc cgatattgat
    80581 cttagatgag ataacagcaa gccttgatgt taacaacgag aaaaagattc aagagtcttt
    80641 aaataattta gttaaagata aaactgttgt aatcatttca catagaatga aatccataga
    80701 aaatgcagac aagatagtag ttcttcaaaa cggaagagta gaaagcgaag gtaagcatga
    80761 agagctttta caaaaatcaa aaatttacaa aaatttaata gaaaagacaa aaatggcaga
    80821 agaatttatt tattaggagg actacaatgg ataataaaaa attaaaagta aaagatttag
    80881 taagtatcgg tgtttttggc gtaatttatt ttgccttcat gtttggagtt ggtatgatgg
    80941 gcttgattcc aatattgttc ttaatatacc cgacagtatt agccatagtt gcaggaactg
    81001 ttgttatgtt atttatggct aaggttcaaa agccatgggc actatttata tttggtatga
    81061 tatcaccact tgtgatgttt gcagctggtc atacctacgt agttgtggtt ttatcactta
    81121 tagtaatgat aatagcagaa ttaattagaa agattggtaa ttataattca tttaaataca
    81181 atatgctttc ttatgcaatc ttcagcacat ggatatgtag ctctttaatg caaatgcttt
    81241 tagcaaaaga aaaatatatg gagtggtctt tgatgacgat gggaaaagat tatgttgatg
    81301 tattagaaaa gttaataact tatcctcaca tggctttagt agccttaggt gctttcttag
    81361 gaggaattct tggagcatat ataggcaagg ctctattgaa aaaacacttt gaaaaagcag
    81421 gcattgtata atgcctactt tttcttcaaa tgctcccttt aatcccaatc cgatctctaa
    81481 gatattagtc gtatttatca caggcttaac tgttatgcat agcataaaca tccgttttga
    81541 gctggcaatt gtttgtctta ttggtatttt gctttatttg aatggataca aaaaaacact
    81601 tttcaaatgg attatattgt gtgggatact ttactccttg cctaatttta tggtgttatc
    81661 tgaattaaac cctatagtta agatgttttt gagtatacct attgttatta gaatgtttat
    81721 tttaccattt atggcagcaa gctttatgat aaagacctcg gatgtaggcg caataatttc
    81781 atcgatggat aagcttaaga tttcaaagaa tgtatccata cctattgcgg ttatgtttag
    81841 attcttccca tcttttaagg aggagaagaa aaacatcaaa atggctatga gagtaagagg
    81901 gataagtttt aaaaacccag tcaaatatct tgaatatgtt tctatgccac tactcattat
    81961 atcatctaat atatcagatg acattgcaaa agcggcagaa acaaaggcaa tagaaaatcc
    82021 aattgccaag accagataca ttcgcgtaaa gatacagcta attgattttg tttatgtttt
    82081 agcggttgct ggacttattg tgggaggctt aatatggttg aaataaaaaa tttaagtctt
    82141 gattatggtg aagagcatat attagatgat atatcactat ccatagccga gggagagtgc
    82201 gtgctattta caggaaaaag tggaaatggt aagtcatctt taataaattc aatcaatgga
    82261 ctagctgtaa ggtatgataa cgcaaagaca aagggcgaaa taattattga tggtaagaat
    82321 ataaaaaatt tggaacttta tcaaatctca atgcttgttt caactgtttt tcaaaatcct
    82381 aagacatatt tttttaatgt caatacgaca ttagaattat tattttattt ggaaaatatc
    82441 ggtcttgcaa gagaagagat ggacaggcgt ttgaaggata tgcttaagat attcccgata
    82501 aaaaatcttt tgaacagaaa tatatttaat ctatccggcg gtgaaaaaca aattctttgc
    82561 attgcagctt cttatatagc aggtacaaag attatagtta tggatgagcc ttcatcgaat
    82621 ttagatatta aaagcataag tgttttggca aagatgctaa agatattaaa agagaaaggc
    82681 ataagcataa ttgttgcaga gcatagaatt tattatttga tggacatagt tgaccgtgta
    82741 tttttaatag ataaaggaaa gcttaaaaaa acttatacta gaagtgaatt tttaaagcta
    82801 gataaaaatg aattaaatgc tttaagttta agagataaag aattaagtaa attaaaagtt
    82861 ccttatttaa aagaaggtgg agagtatcag ataaaaaatc ttagctacaa atttactgat
    82921 gatgagtgtt taagcttaaa agatatttca ttcaagcttg ggaaaattta tggcataata
    82981 ggatccaacg gacgaggaaa atcaacgctt ttaagatgtt taataggtct tgagaaaaaa
    83041 tcaaaagaag aaatttattt taagggagag aagctatcta aaaaagaaag actcaaaaac
    83101 tcttcacttg ttatgcaaga tgtaaatcat caattattca cagatgaagt attcaacgag
    83161 cttagattag gagtaaagaa ttttgatgaa gaaaaggcga aaatcatttt aaaagattta
    83221 ggcctggacg aatttattga aaggcatccg atgagtttat caggagggca aaagcaaagg
    83281 cttgcaatag catctgttat gtgcaagaat tctccattta tcttttttga cgaaccttca
    83341 agtggtatgg attattccaa tatgataaaa atatctgaac tgattaataa gtataaaacc
    83401 atggataaaa taatttttat tgtttcccat gatatagaat ttttaaatga agtggcagat
    83461 gaaatttttg aattgtaaaa tagagtctgt taaacatgtc tatagaacta gtaaattgtc
    83521 taaaaattta aaagatacta acacatattg atatttatag aaaattaact ttaggatttt
    83581 tacagtattt ataaacttta taatatgtat ttatagtatt aataatttcc atatctttag
    83641 ggaaattatt tatttactta ccataattaa agagatgaga gataaatatc ggcagtcttc
    83701 ggactgcctt tattttttta cttaatttaa tgcagtaaat catacgattg aaaggaatct
    83761 gcgttatatg ctataattag gtagttaatc tatacattta gaaaggtaaa atatagaatt
    83821 ggagaatgat atgggatttt catataataa attgtaaccg cccaataacg aagtctattg
    83881 aaaaatctcc agattagtga actcacggat agttcctaat ctggagattt cttatttgca
    83941 cttttcttgt acaactttag tccacggtaa ataggcctct aaaacctctt tgtttacgag
    84001 agtttccacg tttggaagac attctagaag ataggataga tatttctcac tatttataat
    84061 ggattgaaat aagatatgaa caaatcgatt aggaaaggaa aattgattta tagaaatgtt
    84121 ttagcagtcg atgcgtactg ttatagattc aacgcactat aattgatgac gtttagctgt
    84181 ttccaacaaa ctcataataa tagctcttgc tttagctcct tcaaaacttt gagaaaataa
    84241 ccaccctcgc ttttttaaac tgagcttagg ctaaaagagt ccactggact cttttactcc
    84301 gtcccataac caatgattta atggcgcatt cagctagatt attggaaagg accagatgtc
    84361 cgtctttcaa aatagtctta aaggtttctt tatacttgag gctatacttg aggctgtatt
    84421 caattgccct tcctagtttt gaacccgata aaactgactg acgccggcac caagcaaaga
    84481 aatcttccat taggggttgg agatgttctt gacgtttctg tagtcgttca tcagctggca
    84541 aagcctccca gtttctttcc aaggaaaata actgattaca ataagctaaa cctttagctc
    84601 ctaaggatga tttattcgct tgcttggggg tcgcttcaaa aaacttcctt ctcacatgta
    84661 cccaacatcc aacaagttca gcttcttcca gttgacaata agcttctagc ggttcgcagt
    84721 ttaccaagct tagcgtcgcc ttgctcctaa accttggact gctatcgcat aggcagaact
    84781 agaggactaa agtcctaagt tactgccgca acatatcaca atgcacatag ccagaataat
    84841 ctcctaggaa ttcttgcact accgaaccac tccgacgctg atcatggtgg tacagcgtga
    84901 ttgcttgatt ctcagctttc ccagacaaaa aggtccaata gtaggtcaga tggctatcat
    84961 tctctagaac ccgataggag gtttcatctg catgaagtaa gggctgaatc aatagtctcg
    85021 ttataaaggg gctttaggaa taggagcttt tacaatttta tcacttggat tattcttact
    85081 gcatgtttgg catttgtaag cgtgttggat atgatccact ctttttaatt gcgcaggaat
    85141 aaagaccaat tcttgtcgtt gaaggctcgc tccaatctct tttaaatcgt cctgacaatc
    85201 agggcaaatg ctctcttcta cttgatgatg aacttcttct gaatcaaatt gggcaagaat
    85261 agcttgacgt ttccctttag atttcttacg tttgtaggta atttcctctg tttcaactgg
    85321 gaacatctcc atctttctca gatggagact cttcctcaaa aaggttgagt tgtccaaaag
    85381 ggcaaacact tttctcagag gattttccag agagcttttg cgttagataa gccacttgtt
    85441 cacacagaag ggaaagttca ttagtgagac tatcaattat agcactctgt tgttgaatga
    85501 ttttcaatct tctcccatag actttcctcc ttatttccta actttattat atagtgtatt
    85561 gaatctataa cagtacacct tgactgctaa aatatttcta taaattaatt tgactttcct
    85621 gatagagatg ttcatatctt atttcaattc actatactaa aaagaaagtc ctcatttcaa
    85681 tagaaatcac gactttctga taaatttatt ttgggagtga tagaaaagcc cttcataagc
    85741 caatctactt gttcaggtgt gagagctttg acatcctttt ctgtactggg ccaagtcagt
    85801 ctgccgttct caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct
    85861 ttaaagcggt ctttacgtcc accacaaaag agaaaaactt gaccggagaa aggatccaat
    85921 tcaaagtggg ttttaaccag ataagccagt gaatcgattc cttgtctcat atcagtcttc
    85981 ccacacacga gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt
    86041 atccaaaagc tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt
    86101 gccaatacga attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg
    86161 tggaatctct acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt
    86221 actaatagta tacaggagga actccatgaa tgatagatac cttgttatga cgcgcttact
    86281 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
    86341 gtttgaaaac tacggtctaa ttttttgtcc aaatgtaaat cacttggaac tattattcaa
    86401 tttattcttt ttagtaaatg ttcccgtttt aatcctctgt aaccgttgat tttacaaagc
    86461 ttttaatttt tttgcgatta ctcccactca actgttgcag gtggtttact tgtaatatcg
    86521 tagacgatac ggttaacatg atccacttca tttacgatac gtactgagat tttttgaagt
    86581 acttcccatg gaattttggc aaaatcagca gtcataccat cgatagaagt gatagcacgg
    86641 attgcaatcg tgtagtcata cgtacgaccg tcacccataa caccgactga acgaacgcct
    86701 gtgttaacag tgaagtattg ccaaatatcg cggtcaagtc cagctttagc gatttcttca
    86761 cgaagaatag cgtctgattc acgaac
//
LOCUS       DAVMMR010000002        75575 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMMR010000002 DAVMMR010000000
VERSION     DAVMMR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.002"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            226..6660
                     /locus_tag="VYS74_000092"
     CDS             226..6660
                     /locus_tag="VYS74_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV3788201.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7292..7768
                     /locus_tag="VYS74_000093"
     CDS             7292..7768
                     /locus_tag="VYS74_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3788202.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7806..8111
                     /locus_tag="VYS74_000094"
     CDS             7806..8111
                     /locus_tag="VYS74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3788203.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            8159..9634
                     /locus_tag="VYS74_000095"
     CDS             8159..9634
                     /locus_tag="VYS74_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV3788204.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            9939..16640
                     /locus_tag="VYS74_000096"
     CDS             9939..16640
                     /locus_tag="VYS74_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV3788205.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIEL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(16888..17744)
                     /locus_tag="VYS74_000097"
                     /pseudo
     CDS             complement(16888..17744)
                     /locus_tag="VYS74_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17955..18314)
                     /locus_tag="VYS74_000098"
     CDS             complement(17955..18314)
                     /locus_tag="VYS74_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV3788206.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            18406..19362
                     /locus_tag="VYS74_000099"
                     /pseudo
     CDS             18406..19362
                     /locus_tag="VYS74_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            19346..19903
                     /locus_tag="VYS74_000100"
     CDS             19346..19903
                     /locus_tag="VYS74_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3788207.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            19896..20150
                     /locus_tag="VYS74_000101"
     CDS             19896..20150
                     /locus_tag="VYS74_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788208.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            20162..22216
                     /locus_tag="VYS74_000102"
     CDS             20162..22216
                     /locus_tag="VYS74_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV3788209.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            22395..23255
                     /locus_tag="VYS74_000103"
     CDS             22395..23255
                     /locus_tag="VYS74_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV3788210.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            23466..24176
                     /gene="ccdA2"
                     /locus_tag="VYS74_000104"
     CDS             23466..24176
                     /gene="ccdA2"
                     /locus_tag="VYS74_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3788211.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            24196..24762
                     /locus_tag="VYS74_000105"
     CDS             24196..24762
                     /locus_tag="VYS74_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV3788212.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            24773..25885
                     /gene="msrB"
                     /locus_tag="VYS74_000106"
     CDS             24773..25885
                     /gene="msrB"
                     /locus_tag="VYS74_000106"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3788213.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEIMLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            26153..26890
                     /locus_tag="VYS74_000107"
     CDS             26153..26890
                     /locus_tag="VYS74_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788214.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            26887..28578
                     /locus_tag="VYS74_000108"
     CDS             26887..28578
                     /locus_tag="VYS74_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3788215.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            28694..29257
                     /locus_tag="VYS74_000109"
     CDS             28694..29257
                     /locus_tag="VYS74_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV3788216.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            29279..34948
                     /locus_tag="VYS74_000110"
     CDS             29279..34948
                     /locus_tag="VYS74_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV3788217.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            35009..36730
                     /gene="pabB"
                     /locus_tag="VYS74_000111"
     CDS             35009..36730
                     /gene="pabB"
                     /locus_tag="VYS74_000111"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV3788218.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            36850..38376
                     /locus_tag="VYS74_000112"
     CDS             36850..38376
                     /locus_tag="VYS74_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3788219.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            38463..39382
                     /locus_tag="VYS74_000113"
                     /pseudo
     CDS             38463..39382
                     /locus_tag="VYS74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            39517..40476
                     /locus_tag="VYS74_000114"
     CDS             39517..40476
                     /locus_tag="VYS74_000114"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV3788220.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEEDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            40573..41412
                     /locus_tag="VYS74_000115"
     CDS             40573..41412
                     /locus_tag="VYS74_000115"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV3788221.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(41460..41630)
                     /locus_tag="VYS74_000116"
     CDS             complement(41460..41630)
                     /locus_tag="VYS74_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV3788222.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            41751..42635
                     /gene="miaA"
                     /locus_tag="VYS74_000117"
     CDS             41751..42635
                     /gene="miaA"
                     /locus_tag="VYS74_000117"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV3788223.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            42628..43866
                     /gene="hflX"
                     /locus_tag="VYS74_000118"
     CDS             42628..43866
                     /gene="hflX"
                     /locus_tag="VYS74_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV3788224.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            43859..44482
                     /locus_tag="VYS74_000119"
     CDS             43859..44482
                     /locus_tag="VYS74_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV3788225.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            44497..45426
                     /locus_tag="VYS74_000120"
     CDS             44497..45426
                     /locus_tag="VYS74_000120"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV3788226.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            45447..46202
                     /locus_tag="VYS74_000121"
     CDS             45447..46202
                     /locus_tag="VYS74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV3788227.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(46256..47164)
                     /locus_tag="VYS74_000122"
     CDS             complement(46256..47164)
                     /locus_tag="VYS74_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3788228.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            47228..47497
                     /locus_tag="VYS74_000123"
     CDS             47228..47497
                     /locus_tag="VYS74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV3788229.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            47497..47877
                     /locus_tag="VYS74_000124"
     CDS             47497..47877
                     /locus_tag="VYS74_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3788230.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            47966..48193
                     /locus_tag="VYS74_000125"
                     /pseudo
     CDS             47966..48193
                     /locus_tag="VYS74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48233..48958)
                     /locus_tag="VYS74_000126"
     CDS             complement(48233..48958)
                     /locus_tag="VYS74_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV3788231.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            49120..50961
                     /gene="typA"
                     /locus_tag="VYS74_000127"
     CDS             49120..50961
                     /gene="typA"
                     /locus_tag="VYS74_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV3788232.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            50981..51235
                     /locus_tag="VYS74_000128"
     CDS             50981..51235
                     /locus_tag="VYS74_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV3788233.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            51599..51898
                     /locus_tag="VYS74_000129"
                     /pseudo
     CDS             51599..51898
                     /locus_tag="VYS74_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            51969..53990
                     /locus_tag="VYS74_000130"
     CDS             51969..53990
                     /locus_tag="VYS74_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3788234.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPSYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILRSRDWVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            53992..54633
                     /locus_tag="VYS74_000131"
     CDS             53992..54633
                     /locus_tag="VYS74_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788235.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLDLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWGQVDQVIRVTDLSHR"
     gene            54737..56089
                     /locus_tag="VYS74_000132"
     CDS             54737..56089
                     /locus_tag="VYS74_000132"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV3788236.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            56091..57149
                     /locus_tag="VYS74_000133"
     CDS             56091..57149
                     /locus_tag="VYS74_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV3788237.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVPFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQALFSKGG
                     FVSVPPVIAACVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            57159..58379
                     /gene="divIB"
                     /locus_tag="VYS74_000134"
     CDS             57159..58379
                     /gene="divIB"
                     /locus_tag="VYS74_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV3788238.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYISVLFNDSEQIKAFT
                     SELAQINPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            58962..59969
                     /locus_tag="VYS74_000135"
     CDS             58962..59969
                     /locus_tag="VYS74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
                     /protein_id="HEV3788239.1"
                     /translation="MRIVDKIKILPTPYEGQYHLYIPSSKKHVLVGKQEKKWLEQLIG
                     QEFTISDLLVLVGKKYYLRFYIVLSKMGLIDSMKSRKKINIFKISWDFNITLSEKLNY
                     KRFNKNFEKILFGIFPLALILQCFIMYIYRADILTNFQNFPITLNILLFWLLVVIISA
                     TIHEFSHAIIANNYQIEIPNCGIMLLLFNLAFFVDLSGVQFLKNKKQFLKIMLAGIAS
                     NSILSLTGLLVLLFTSYKLLGLLFFIINLGMIFINSIPFFQYDSGIILRYLNSNNNNL
                     NFQYIVQLLIAFVIIYFPLSQLLQFLTPNIIVRSIGGVVVSILLSILYMRGRTKYVLR
                     K"
     gene            59953..60141
                     /locus_tag="VYS74_000136"
                     /pseudo
     CDS             59953..60141
                     /locus_tag="VYS74_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            60178..61326
                     /locus_tag="VYS74_000137"
     CDS             60178..61326
                     /locus_tag="VYS74_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV3788240.1"
                     /translation="MDSRYSRTKGYYSLFYNEEYNKIQNKTVLVLGAGALGCYISLSL
                     SMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSQKIHKYNSDVQVVPISI
                     KVSSVEELENIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKISYISGGFNGCYLII
                     DNIYIPTIGSCFACRNINKDINKYTLSDKTKWPTTPEMPAILGGIMTNLIIKIFLGCY
                     NEILIDNAYVYNMRNHALSQEKYVLENGECPICKKNNKVKDNNIRAKTFIRSVCFCLL
                     SGGVAFLSAIGQFTVIETQLIVLFLGIIFAIYYAYYNKNIQTSLENIVWLFSSFEILF
                     LLVNFRTFIQLPVDIFISMIIFLMLWIFIMLGIVCLSYYITLLFSKEA"
     gene            61536..62369
                     /locus_tag="VYS74_000138"
     CDS             61536..62369
                     /locus_tag="VYS74_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788241.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
     gene            62362..63075
                     /locus_tag="VYS74_000139"
     CDS             62362..63075
                     /locus_tag="VYS74_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788242.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(63397..63489)
                     /locus_tag="VYS74_000140"
                     /pseudo
     CDS             complement(63397..63489)
                     /locus_tag="VYS74_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            63810..64511
                     /gene="pyrF"
                     /locus_tag="VYS74_000141"
     CDS             63810..64511
                     /gene="pyrF"
                     /locus_tag="VYS74_000141"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV3788243.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            64545..65177
                     /locus_tag="VYS74_000142"
     CDS             64545..65177
                     /locus_tag="VYS74_000142"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV3788244.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            65357..65762
                     /locus_tag="VYS74_000143"
                     /pseudo
     CDS             65357..65762
                     /locus_tag="VYS74_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            65858..66622
                     /locus_tag="VYS74_000144"
     CDS             65858..66622
                     /locus_tag="VYS74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788245.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            66622..67374
                     /locus_tag="VYS74_000145"
     CDS             66622..67374
                     /locus_tag="VYS74_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788246.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLALLSIIIPLTVFSVHRNWRRV"
     gene            67384..68082
                     /locus_tag="VYS74_000146"
     CDS             67384..68082
                     /locus_tag="VYS74_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788247.1"
                     /translation="MGKLSSHIWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            68079..68726
                     /locus_tag="VYS74_000147"
     CDS             68079..68726
                     /locus_tag="VYS74_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788248.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(68806..69666)
                     /locus_tag="VYS74_000148"
     CDS             complement(68806..69666)
                     /locus_tag="VYS74_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3788249.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGPSSGKTTAKARTID
                     ENKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(69678..70435)
                     /locus_tag="VYS74_000149"
                     /pseudo
     CDS             complement(69678..70435)
                     /locus_tag="VYS74_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(70436..71113)
                     /locus_tag="VYS74_000150"
     CDS             complement(70436..71113)
                     /locus_tag="VYS74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3788250.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQSLGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(71073..71740)
                     /locus_tag="VYS74_000151"
                     /pseudo
     CDS             complement(71073..71740)
                     /locus_tag="VYS74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            71966..72112
                     /locus_tag="VYS74_000152"
     CDS             71966..72112
                     /locus_tag="VYS74_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV3788251.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            72240..73730
                     /gene="lysS"
                     /locus_tag="VYS74_000153"
     CDS             72240..73730
                     /gene="lysS"
                     /locus_tag="VYS74_000153"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV3788252.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            74349..75485
                     /gene="lctO"
                     /locus_tag="VYS74_000154"
     CDS             74349..75485
                     /gene="lctO"
                     /locus_tag="VYS74_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV3788253.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT        23980 a        13070 c        16117 g        22408 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca gttattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacactaa aactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagataatgc acactcgtct gcaaataaag gcacagaaaa tccgaaaaaa
      601 gaagatagac ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 acatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgca gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctattcgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat tattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagact taaagaaact cattaaaaag
     4441 aaatttagag aagttgatga ttttacaagt gaaactggta agagaataga ggaatacgat
     4501 tataaatacg atgataaggg aaatattatt gcttatgacg atggtagtgc cttacaatat
     4561 gaaactgaaa aacttgacga aataaaatca aaaatttatg gcgttctaag cccatctaaa
     4621 gatggacact ttgaaattct tggaaagata agcaatgttt ctaaaaatgc caaggtatat
     4681 tatggaaata gctataaatc gatagaaatc aaagcgacta agtatgactc acattcaaaa
     4741 acgatgacat ttgatttata cgctaatatt aatgatattg tggatggatt agcttttgct
     4801 ggagatatgc ggttttttgt taaagatgat aatcgaataa aagctgaaac taaaattaga
     4861 atgcctgaaa aaaataagga aactaaagca gaatatccct atgtatcaag ttatgggaat
     4921 gtaatcgaat taggagaagg agatctttca aagaacaaac cagacaattt aactaaaatg
     4981 gaatctggta aaatctattc tgattcagaa aaacaacaat atctgttaaa ggataacatc
     5041 attctaagaa aaggctatgc actaaaagtg actacctata atcctggaaa aacggatatg
     5101 ttagaaggaa atggagtcta tagcaaggaa gatatagcaa aaatacaaaa ggccaatcct
     5161 aatctaagag tcctttcaga aacaacaatt tatgctgata gtagaaatgt tgaagatgga
     5221 agaagtaccc aagctgtatt aatgtcggct ttggacggct ttaacattat aaggtatcaa
     5281 gtgtttacat ttaaaatgaa cgataaaggg gaagctatcg ataaagacgg aaatcttgtg
     5341 acagattctt ctaaacttgt attatttggt aaggatgata aagaatacac tggagaggat
     5401 aagtccaatg tagaagctat aaaagaagat ggctccatgt tatttattga taccaaacca
     5461 gtaaaccttt caatggataa gaactacttt aatccatcta aatctaataa aatttatgta
     5521 cgaaatccag aattttattt aagaggtaag atttctgata agggtggttt taactgggaa
     5581 ttgagagtta atgaatcggt tgtagataat tatttaatct acggagattt acacattgat
     5641 aacactagag attttaatat taagctgaat gttaaagacg gtgacatcat ggactgggga
     5701 atgaaagact ataaagcaaa cggatttcca gataaggtaa cagatatgga tggaaatgtt
     5761 tatcttcaaa ctggctatag cgatttgaat gctaaagcag ttggagtcca ctatcagttt
     5821 ttatatgata atgttaaacc cgaagtaaac attgatccta agggaaatac tagtatcgaa
     5881 tatgctgatg gaaaatctgt agtctttaac atcaatgata aaagaaataa tggattcgat
     5941 ggtgagattc aagaacaaca tatttatgta aatggaaaag aatatacatc atttgatgat
     6001 attaaacaaa taacagacaa gacactaaac attaagattg ttgtaaaaga ttttgcaaga
     6061 aatacaaccg taaaagaatt cattttaaat aaagatacgg gagaggtaag tgaattaaaa
     6121 cctcataggg taactgtgac cattcaaaat ggaaaagaaa tgagttcaac gatagtgtcg
     6181 gaagaagatt ttattttacc tgtttataag ggtgaattag aaaaaggata ccaatttgat
     6241 ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg gctatgttat taatctatca
     6301 aaagatacct ttataaaacc tgtattcaag aaaatagagg agaaaaagga ggaagaaaat
     6361 aaacctactt ttgatgtatc gaaaaagaaa gataacccac aagtaaacca tagtcaatta
     6421 aatgaaagtc acagaaaaga ggatttacaa agagaagatc attcacaaaa atctgattca
     6481 actaaggatg ttacagctac agttcttgat aaaaacaata tcagtagtaa atcaactact
     6541 aacaatccta ataagttgcc aaaaactgga acagcaagcg gagcccagac actattagct
     6601 gccggaataa tgtttatagt aggaattttt cttggattga agaaaaaaaa tcaagattaa
     6661 gacaaaagct atagaaaaaa atggtttatg tactgagatt agatagtgag gtgatgacat
     6721 agttttgtga aaacggccat ttataactca attatttagt ttacttcact tcactattga
     6781 tactatttgg agttattaat ggacttagtt tagataacta atgaattgat tgaaagggtt
     6841 agtattgaca atattggtca tattgattag aaaatagagt ctatcaaaat ttaaaggcta
     6901 atagaggtga tgagacaatt tcaaggaatt aagtagcgtt tggttgctta attccttttt
     6961 atttggaagg aagtttttcg taaccgccca ataacgaagt ctattggacg gttacgatat
     7021 gatcactgac ttcgtcagtc ttatctacaa cctcaaaact gtgttttgag caacctgcgg
     7081 ctagcttcct agtttactct ttgattttca ttgagtatta gaacagaaaa aatgcttgga
     7141 gtatttgttt gtgtgtttat ttttatataa caaactataa acaaaacaaa aatataaaaa
     7201 aagagacaaa aaagaacaaa aagtaattga caacggtttc atatagtgtt atacttatgg
     7261 cataaagtta atggaaagaa gggaaaaact tatggtatta gattatttct ttgacaaaaa
     7321 ccttgtgttt tgcttagaag cggataatca agaacatctc tttgatcagg ttgcaacttt
     7381 attggaagaa cgagaaattg taactccaac ttatcgtgaa gccttgatca cgcgtgaaaa
     7441 gtcatttcca actggtttag atatggaatt tctaggaaag gacttgccaa atgtagcgat
     7501 tcctcacaca gatattgttc ataatctagc tgagaaagtg gtggttgttc gattagaaaa
     7561 accagtaact tttcacaata tgatagctcc tgataaggaa gtagaagtat ctctactctt
     7621 ctttatcatt aacaattcaa gttcaagtca aacgaacatt ctggctcagt tgatggactt
     7681 tttcacagga aatggtcatc ttgaagacct atcaaaaatt tctgaaccag aaaaacttta
     7741 tgcttacatt gctgaagcaa ccgcttaatc ttgtctatta aaaaataaaa tcggaggaaa
     7801 tccaaatgat taaaattctt gctgcctgcg gtgcaggtgt taactcaagt caccaaatta
     7861 aaagtgctct agaagaagaa ctttcaaacc gtggttatga tgttcactgt gatgcagtca
     7921 tggtgaaaga tgtaaacgaa gaccttatga aaggttacga tatctttaca ccaatcgctg
     7981 caacagacct tggttttgaa ccaggtatcc cagttatcga agctgggcca atcttattcc
     8041 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacatg
     8101 gattaagcta attctaattt gttaacattc atataacata aaaaagggag gaactgttat
     8161 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
     8221 tgtcatcatg ttgatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
     8281 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
     8341 tatgctaaat ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca
     8401 attgagtatt acagacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg
     8461 gacactatac ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa
     8521 aaccgataca cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt
     8581 gattaaatgg tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac
     8641 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
     8701 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
     8761 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
     8821 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
     8881 attcttcatc ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat
     8941 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
     9001 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     9061 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc gaggacgtaa
     9121 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     9181 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     9241 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     9301 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     9361 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     9421 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     9481 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     9541 agtagtcttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     9601 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     9661 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctaaaaacac agttttgagg
     9721 ttgtagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
     9781 acgaagtcag taatcatatc tacggcaagg cgacgctgac gaagtttgaa ttggattttc
     9841 gcagagtgta aattgttgaa actaggttgt cataccatgt ataccacttg ggctttggta
     9901 gtagctaact gcactaaata taatataagg agggttttat ggggaaaggc cattggaatc
     9961 ggaaaagagt ttatagcatt cgtaagtttg ctgtgggagc ttgctcagta atgattggga
    10021 cttgtgcagt tctattagga ggaaatatag ctggagaatc tgtagtttat gcggatgaaa
    10081 cacttattac tcatactgct gagaaaccta aagaggaaaa aatgatagta gaagaaaagg
    10141 ctgataaagc tttggaaact aaaaatgtag ttgaaaggac agaacaaagt gaacctagtt
    10201 caactgaggc tattgcatct gagaagaaag aagatgaagc cgtaactcca aaagaggaaa
    10261 aagtgtctgc taaaccggaa gaaaaagctc caaggataga atcacaagct tcaagtcaag
    10321 aaaaaccgct caaggaagat gctaaagctg taacaaatga agaaatgaat caaatgattg
    10381 aagacaggaa agtggatttt aatcaaaatt ggcactttaa actcaatgca aattctaagg
    10441 aagccattaa acctgatgca gacgtatcta cgtggaaaaa attagattta ccgtatgact
    10501 ggagtatctt taacaatttc gatcatgaat ctcctgcaca aaatgaaggt ggacaactca
    10561 acggtgggga agcttggtat cgcaagactt tcaaactaga tgaaaaagac ctcaagaaaa
    10621 atgttcgcct tacttttgat ggcgtctaca tggattctca agtttatgtc aatggtcagt
    10681 tagtggggca ttatccaaat ggttataacc agttctcata tgatatcacc aaataccttc
    10741 acaaagatgg tcgtgagaat gtgattgctg tccatgcagt caacaaacag ccaagtagcc
    10801 gttggtattc aggaagtggt atctatcgtg atgtgacttt acaagtgaca gataaggtgc
    10861 atgttgagaa aaatgggaca actattttaa caccaaaact tgaagaacaa caacatggca
    10921 aggttgaaac tcatgtgacc agcaaaatcg tcaatacgga cgacaaagac catgaacttg
    10981 tagccgaata tcaaatcgtt gaacgaggtg gtcatgctgt aacaggctta gttcgtacag
    11041 cgagtcgtac cttaaaagca catgaatcaa caagcctaga tgcgatttta gaagttgaaa
    11101 gaccaaaact ctggaccgtt ttaaatgaca aacctgcctt gtacgaattg attacgcgtg
    11161 tttaccgtga cggtcaattg gttgatgcta agaaggattt gtttggttac cgttactatc
    11221 actggactcc aaatgaaggt ttctctttga atggtgaacg tattaaattc catggagtat
    11281 ccttgcacca cgaccatggg gcgcttggag cagaagaaaa ctataaagca gaatatcgcc
    11341 gtctcaaaca aatgaaggag atgggagtta actctatccg tacaacccac aaccctgcta
    11401 gtgagcaaac cttgcaaatc gcagcagaac taggtttact cgttcaggaa gaggcctttg
    11461 atacgtggta tggtggcaag aaaccttatg actatggacg tttctttgaa aaagatgcca
    11521 ctcacccaga agctcgaaaa ggtgaaaaat ggtctgattt tgacctacgt accatggtcg
    11581 aaagaggcaa aaacaaccct gctatcttca tgtggtcaat tggtaatgaa ataggtgaag
    11641 ctaatggtga tgcccactct ttagcaactg ttaaacgttt ggtcaaggtt atcaaggatg
    11701 ttgataagac tcgctatgtt accatgggag cagataaatt ccgtttcggc aatggtagcg
    11761 gagggcatga gaaaattgct gatgaactcg atgctgttgg atttaactat tctgaagata
    11821 attacaaagc ccttagagct aagcatccaa aatggttgat ttacggttca gaaacatcat
    11881 cagcaacccg tacacgagga agttactatc gccctgaacg tgaattgaaa catagcaatg
    11941 gacctgagcg taattatgaa cagtcagatt atggaaatga tcgtgtgggt tgggggaaaa
    12001 cagcaaccgc ttcatggact tttgaccgtg acaacgctgg ctatgctgga cagtttatct
    12061 ggacaggtac ggactatatt ggtgaaccta caccatggca caaccaaaat caaactcctg
    12121 ttaagagctc ttactttggt atcgtagata cagccggcat tccaaaacat gacttctatc
    12181 tctaccaaag ccaatgggtt tctgttaaga agaaaccgat ggtacacctt cttcctcact
    12241 ggaactggga aaacaaagaa ttagcatcca aagtagctga ctcagaaggt aagattccag
    12301 ttcgtgctta ttcgaatgct tctagtgtag aattgttctt gaatggaaaa tctcttggtc
    12361 ttaagacttt caataaaaaa caaaccagcg atgggcggac ttaccaagaa ggtgcaaatg
    12421 ctaatgaact ttatcttgaa tggaaagttg cctatcaacc aggtaccttg gaagcaattg
    12481 ctcgtgatga atctggcaag gaaattgctc gagataagat tacgactgct ggtaagccag
    12541 cggcagttcg tcttattaag gaagaccatg cgattgcagc agatggaaaa gacttgactt
    12601 acatctacta tgaaattgtt gacagccagg ggaatgtggt tccaactgct aataatctgg
    12661 ttcgcttcca attgcatggc caaggtcaac tggtcggtgt agataacgga gaacaagcca
    12721 gccgtgaacg ctataaggcg caagcagatg gttcttggat tcgtaaagca tttaatggta
    12781 aaggtgttgc cattgtcaaa tcaactgaac aagcagggaa attcaccctt actgctcact
    12841 ctgatctctt gaaatctagt caagtcactg tctttactgg taagaaagaa ggacaagaaa
    12901 agactgtttt ggggacagag gtgccaaaag tacagaccat tattggagag gcacctgaaa
    12961 tgcctaccac tgttccgttt ctatacagtg atggtagtcg tgcagaacgt cctgtaacct
    13021 ggtcttcagt agatgtgagc aagcctggta ttgtaacggt gaaaggtatg gctgacggac
    13081 gagaagtaga agctcgtgta gaagtgattg ctcttaaatc agagctacca gttgtgaaac
    13141 gtattgctcc aaatactgac ttgaattctg tagacaaatc tgtttcctat gttttgactg
    13201 atggaagtgt acaagagtac gaagtagata gctgggagat tacggaagta gataaagcca
    13261 aactttcagt agctggatca cgtattcaaa tgactggtca gttagctgga gaaactattc
    13321 atgcaaccct tgtggtagaa gaaggaaatg ctgcagcacc tgtagtgcca actgttactg
    13381 ttggaggtga agcagtaaca ggtcttacta gtcgacaacc aatgcaatat cgtactctat
    13441 cttatggtgc ccaattgcca gaagtcacag caagtgctga aaatgctgat gtgacagttc
    13501 ttcaagcaag cgcagcaaac ggcatgcgtg caagcatatt tgttcagcct aaagatggtg
    13561 gccctcttca aacctatgca attcaattcc tagaagaagc accaaaaatt gctcacttga
    13621 gcttgcaagt ggaaaaagct gacagtctca aagaagacca aactgtcaaa ttgtcggttc
    13681 gagctcacta tcaagatgga acgcaagctg tattaccagc tgataaagta accttctcta
    13741 caagtggtga aggggaagtc gcaattcgta aaggaatgct tgagttgcat aagccaggag
    13801 cagtcactct caaagcggaa tatgagggag ctaaaggcca agttgaactc actatccaag
    13861 ccaatactga gaagaagatt gcgcaatcta tccgtcctgt aaatgtagtc acagatttgc
    13921 atcaaaaacc tactcttcca acaacagtaa cggttgagta tgacaaaggt ttccctaaag
    13981 ctcacaaagt cacttggcaa gctattccga aagaaaaact agactcctat caaacatttg
    14041 aagtactagg taaagttgaa ggaattgaac ttgaagcgcg tgcaaaagtc tctgtagaag
    14101 gtatcgtttc agttgaagaa gtcagtgtga caactccaat cgcagaagca ccacaattac
    14161 cagaaagtgt tcggacatat gattcaaatg gtcacgtttc atcagctaag gttgcatggg
    14221 atgcgattcg tccagagcaa tacgctaagg aaggtgtctt tacagttaat ggtcgcttag
    14281 aaggtactca attaacaact aaacttcatg ttcgcgtatc tgctcaaact gagcaaggtg
    14341 caaacatttc tgaccaatgg accggttcag aattgccact tgcctttgct tcagactcaa
    14401 atccaagcga cccagtttca aatgttaatg acaagctcat ttcctacaat aaccaaccag
    14461 ccaatcgttg gacaaactgg aatcgtacta atccagaagc ttcagtcggt gtcctattcg
    14521 gagattcagg tattttgagc aaacgttcag ttgataactt aagcgttggc ttccatgaag
    14581 accatggagt tggtgcaccg aagtcttatg tgattgagta ttatgttggt aagactgtcc
    14641 caacagctcc taaaaaccct agttttgttg gtaatgagga ccatgtcttt aatgattctg
    14701 ccaactggaa accagttact aatctaaaag cccctgctca actcaaggct ggagaaatga
    14761 accactttag ctttgataaa gttgaaacct atgctgttcg tattcgcatg gtgagagcag
    14821 acaacaaact aggaacgtct atcacagagg tacaaatctt tgcgaaacaa gttgcggcag
    14881 ccaagcaagg acaaacaaga atccaagttg acggtaaaga cttagcaaac ttcaaccctg
    14941 atttgacaga ctactacctt gagtctgtag atggaaaagt tacggcagtc acagcaagtg
    15001 ttagcaacaa tggcctcgct accgtcgttc caagcgttcg tgaaggtgag ccggttcgtg
    15061 tcatcgcgaa agctgaaaat ggcgacatct taggagaata ccgtctgcac ttcactaagg
    15121 ataagaactt actttctcat aaaccagttg ctgcggttaa acaagctcgc ttgctacaag
    15181 taggtcaagc acttgaattg ccgactaagg ttccagttta cttcacaggt aaagacggct
    15241 acgaaacaaa aaacctgaca gttgaatggg aagaagttcc agcggaaaat ctgataaaag
    15301 caggtcaatt taccgttcga ggccatgtcc ttggtagtga tcttgttgct gaggtcactg
    15361 tacgagtgac agacaaacta ggcgaagctc tttcagataa ccctaactat gatgaaaaca
    15421 gtaaccaggc ctttgcttca gcaaccaatg atattgacaa aaactctcat gaccgcgttg
    15481 actatctcaa tgacggagat cattcagaaa atcgtcgttg gacaaactgg tctccaacac
    15541 catcttctaa tccagaagta tcagcgggtg tgatcttccg tgaaaatggt aagattgtag
    15601 aacggactgt tgctcaagcc aaacttcact tctttgcaga tagtggtacg gatgcaccaa
    15661 ctaaactcgt tttagaacgc tatgtcggtc cagagtttga agtgccaacc tactattcaa
    15721 actaccaagc ctacgacgca gaccatccat tcaacaatcc agaaaattgg gaagctgtgc
    15781 cttatcgtgc ggataaagac atcgcagctg gtgatgaaat caacgtaaca tttaaagctg
    15841 tcaaagccaa agctatgaga tggcgtatgg agcgtaaagc agataagagc ggtgttgcga
    15901 tgattgagat gaccttcctt gcaccaagtg aattgcctca agaaagcacg caatcgaaga
    15961 ttcttgtaga tggaaaagaa cttgctgatt tcgctgaaaa tcgtcaagac tatcaaatta
    16021 cctataaagg tcaacggcca aaagtctcag ttgaagaaaa caatcaagta gcttcaactg
    16081 tggtagatag tggagaagat agccttccag tacttgttcg cctcgtttca gaaagtggaa
    16141 aacaagtcaa ggaataccgt atccagttga ctaaggaaaa accagtttct gagaagacag
    16201 ttgctgctgt acaagaagat cttccaaaac tcgaatttgt tgaaaaagat ttggcctaca
    16261 agacagttga gaaaaaagat tcaacactgt atctaggtga aactcgtgta gaacaagaag
    16321 gaaaaactgg taaagaacgt atctttacag cgattaatcc tgatggaagt aaggaagaaa
    16381 aactccgtga agtggtagaa gctccgacag accgcatcgt cttggttgga accaaaccag
    16441 tagctcaaga agctaaaaaa ccacaagtgt cagaaaaagc agatacaaaa ccaattgatt
    16501 caagtgaagc tagtcaaact aataaagccc agttaccaaa tacaggtagt gcggcaagcc
    16561 aagcagcagt agcagcaggt ttagctcttc taggtttgag tgcaggatta gtagttacta
    16621 aaggtaaaaa agaagactag aaattttgat accttcttta tcattaaaga ccaaagaaaa
    16681 actcttgtcc taaatagaag ataaagagtc tggaacaaaa tagttctcaa ccacaaaaaa
    16741 gctagatatt tccaattgcg gaactctagc tttttaattt tgagtcgttc tcttattgta
    16801 tagtagattg aaactagagt agtacacttc tacttctaaa acattgttag aaatcgattt
    16861 gactgtcctt atcgatttgt cctgttctta tttcatttca ctatgtttta ggaggttatt
    16921 ggccataagt accaatccca tgtcaattct cacttgacgc ttgcctctca gattacatct
    16981 cttgtaaccc aaacaagcct ttatctgccc aaagacaggt tctacatcaa tcttgcgttg
    17041 agcgaaaatc tgtctatctt ggggagataa aagcgcctga cattctttag ctttcaagtt
    17101 ttgatagcgt tcgttcatat acaatccctt ttgaggggct gattcaggtt catcagcgta
    17161 gtaaaccttg atttcctgtt gaaagtctgt ctgtgttttc tggtacttga tatggtgaaa
    17221 acgatagcac cagccatcag gatgtatgta gctatcctcc ttgtcattat agtgccaatt
    17281 cgctaagttt ttagctgact gtttataccc tctcttttgt tccttatcaa acatggcata
    17341 tttaatcaga aggggagtga tttcttcttg aaaatagact tgtatctgtt cttgaagttt
    17401 ggcggagaat ttctctgtcg ttttcttcca cacaaaacta tacttgttgg cattggattc
    17461 aatcttagtt ccgtcaataa acaaacaatc taaggtcact aactcttcca tttttcaatt
    17521 tttcgaccag agaaaatccc ttgtgaatag gcaaatagaa gagtagatac aagcatttta
    17581 gggtgataag acgggcgacc aaaggcatga taggaggtgt agaagtgacg ttcctccaag
    17641 gtattcacca ctttttcaat agtaaagacg agatggtctt gtggtaagaa ggaactgatt
    17701 tctagtggta aagttgtttg atttgtgtta tagtgaatat gcatgggata gcctttctaa
    17761 atggtttatt aagcaattct attttaccaa gactatcgta accatgcaaa aaagcaacgg
    17821 caaatccgtt gccttttttg gatataagag actattgtcc cagactcttt tctttgtata
    17881 aaattgtata gtgttattgt ctatactatt ttgtagagaa agcaaaaatc cctagaaaat
    17941 gcttagggat tcgactattt cattaagaac aattcaacaa tcatctctat atacatgata
    18001 agggttaata aaaatcctgt acgccagaag aatttgatga atttaggata gtaaaagctg
    18061 cgttttttta aaaggaagaa aaagacgaga ataatggcta ggaacgagag ggctagcccc
    18121 agtctaggga ggaaattatg ggtaaaggtt ttagctgtaa tcaagtaata ctcaaatacc
    18181 aagactggaa aagccaaatc cgcaaagttt cgtcctagtt tttttaatct aaaaagtttg
    18241 gttaggataa tgcagacgac taaggtcagt atcaataata aaatagatgc taatttcatt
    18301 aaaatcatac ccatattgta tcataaaaaa aggctaatgg aaacgagaaa cagaggaatt
    18361 tataggaaag tcaggctttt gagattgtgg gtgatttttg ttataatgaa agttataaaa
    18421 tcttatgata ccttgaatga ttattatcga aaactctttg gagaaaagac ttttaaagtc
    18481 cctattgatg cgggatttga ctgtcccaat cgtgatggaa ctgtggctca tggaggctgt
    18541 actttttgta cggtttccgg ttctggagat gccattgtag ccccagatcc gcctatccgt
    18601 gagcaatttt ataaggaaat tgactttatt catcgtaagt ggccagatgt tcagaagtat
    18661 ctggtttatt ttcaaaattt taccaacacc catgaaaagg tggaagttat tcgagagcgt
    18721 tatgaacagg ctatcaatga gccaggtgtg gtaggaatca atattggaac gcgcccagac
    18781 tgtttaccag acgaaaccat cgaatatttg gctgagttat cggagtgcat gcatgtgacg
    18841 gttgaattgg gcttgcagac tacttatgaa gcaacctctg acctgattaa ccgtgcgcat
    18901 tcttatgaat tgtaggtgga aacggtcaag cgtttgagaa agtatccaaa aattgagatt
    18961 gtttcccatt tgatcaatgg tttgcctggt gaaacccatg agatgatggt tgaaaatgtc
    19021 cgccgttgtg tcacggataa cgatattcaa gggattaaac tgcatttgct ccatcttatg
    19081 acaaatactc gtatgcaacg agattaccat gagggacgtt tacaattgat gagtcaggac
    19141 gaatatgtca gggtcatctg tgaccaactg gaaattattc ccaaacatat cgtcatccat
    19201 cgaattacag gagatgcgcc tagggatatg ctgattggtc ctatgtggag cctcaataaa
    19261 tgggaagtcc tcaacagcat tgagatggag atgagacgtc gtggaagtgt tcaaggatgc
    19321 aaggctgtaa aacaggagtt tgaaaatgaa aagaccactt gagatggcac atgatttttt
    19381 ggctgaggtc gtgacaaaag aggatgtcgt agtggatgcg actatgggaa atggtcatga
    19441 cacgcttttt ttagccaagc tagccaagca agtctatgcc tttgatattc agaagcaagc
    19501 cttggaaaag acccaagagc gtttgcatca ggctggcttg acaaatgccc agttaatctt
    19561 gcaaggccat gagacactgg accagtttgt gacaaaagct aaggcaggga tttttaattt
    19621 gggctatttg ccgtcagctg ataagtctgt catcacccga ccgcagacaa cgattgaggc
    19681 attagaaaag ctatgtggct tacttgtcaa aggtggacga attgctatta tgatttacta
    19741 tggtcatgaa ggaggcgacc tcgagagaga tgctgtcttg gattttgtga tccagttgaa
    19801 ccaacaagag tacacagctg ccatttaccg aactttaaac caagtcaaca acccgccgtt
    19861 tttagtgatg attgaaaaat tagagagata cagacatgga taaacaatac ctacacgaaa
    19921 agctggatgc catgcgccag aattttgttg aatcaaccta ccacgaacga gcggtgggcg
    19981 tgctagacca agcgcatatg agcaaaaaaa tgcttaaaat caagaaaaaa ttagttgctc
    20041 ttgaaatgga acggtgccag agaaaaattg agcacaaaga ctgttccaag attgaccaaa
    20101 aaatcaaaga gcagaaggag atatttgaat cctgttgtaa aaaagattaa ggaggacgtg
    20161 cgtggaatta ctgatttacc tcatcctatt tttactggtc ttgattgtct caagtacaac
    20221 caataagctc ctaccctttt tgcctctccc tttggtgcag attcttttgg gaattgtgat
    20281 tggtctcttt ttacccaata ctgactttca ccttaatacg gagttgtttt tggcactggt
    20341 tatcggcccc ttgcttttcc gagaggctga agaagcagat gttacggcta ttttaaaaca
    20401 ctggcgaatc attgtttatc tcatatttcc agtgattttt atctcgaccc tgagtttggg
    20461 tggcttggcc catcttcttt ggttcagcct tcccttggca gcttgcttgg ctgttggggc
    20521 agcccttggt cctacggact tggtggcctt tgcctctctt tcggagcgtt ttagctttcc
    20581 taagcgcgtg tccaatattc ttaagggcga aggactcttg aatgatgctt ctggtttggt
    20641 ggcttttcag gtagctttga cagcttggac aactggagct ttttctctgg ggcaagctag
    20701 cagttcactc atcttttcaa tcctaggcgg ttttttaatt ggatttttaa cagctatgac
    20761 caaccgcttc ctccatacct tcttgctaag tgtgcgcgca acggatattg ccagtgaact
    20821 tttattagag ttgagtttgc ctctagtgac cttctttctg gcagaagaag tccatgtttc
    20881 agggattatt gccgtcgtag ttgctggaat tttaaaggca agtcgcttca agaaaatcac
    20941 gctcctcgaa gcccaagtgg atacggtgac cgagacggtc tggcatacag tgacctttat
    21001 gctcaacggt tctgtctttg tgattttagg gatggagttg gaaatgatag cagaacctat
    21061 cttgaccaat ccaatctata atcctctact tttattgcta tctctcatcg cccttacctt
    21121 tgtcctcttt gtcattcgtt ttattatgat ctatggctat tatgcctata gaacccgacg
    21181 cctcaagaaa aagctaaata agtatatgaa ggacatgttt ctcttgacct tttcaggtgt
    21241 taagggaacg gtgtcgattg ctacgattct cttgatacca agtaatctag aacaggagta
    21301 tcctctcttg cttttccttg ttgcaggtgt gacgcttgtc agctttttaa caggtctctt
    21361 ggtcttgcct catctttctg atgaagagga agaaagcaag gattatctca tgcatatcgc
    21421 cattttgaat gaagtaacgc tagagttgga aaaagagttg gaagacacca gaaataaact
    21481 tcccctctat gcggctattg acaattatca tggacgtatt gaaaatctca ttttaagcca
    21541 agaaaaccag gatgatcaag aagactgggc tgctttgaaa ctcttgattc ttagtattga
    21601 aagtgatggt ttggaacagg cctatgaaga ggggaacatt agcaatcgtg cttaccgagt
    21661 ttaccaacgt tatctgaaaa atatagaaca aggaatcaat cgtaaacttg cctcaagact
    21721 gacctattat tttcttgttt ccttgaggat tttacgtttt ctccttcatg aagtttttac
    21781 tcttggaaag accttccgta gctggaagga caaggagcaa agccgtctcc gtgctcttga
    21841 ttatgaccaa attgcagagc tctatcttgc caatacagag atgattattg aaagtttgga
    21901 aaacctgaag ggagtctaca gacgctcttt gattagtttt atgcaggagt ctcgtcttcg
    21961 agaaacagct attatcagca gtggtgcctt tgtcgaacgg gttatcaatc gtgtcaaacc
    22021 caacaatatc gatgaaatgc tgagaggcta ttatctggag cgcaagttga ttttcgaata
    22081 cgaagaaaaa cgattgatta cgactaagta tgccaagaaa ttacgacaaa atgtaaataa
    22141 cttagagaac tattccttga aggaagctgc caatacccta ccgtatgata tggtggaatt
    22201 ggtaagaaga aattagttaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    22261 taccgtatgt atgtgactga cttcgtcagt cttatctaca acctcaaaac agtgttttga
    22321 gcagcttgcg gctagctttc tagtttgctc tttgattttc attgagtata agattataag
    22381 tgaaggagta tgacatgaaa aagtggtgga aagagctgat agacaaacct ttattaaaag
    22441 cttttttgca ttattatcaa gcatcagata gtgagttgac cagtgtcgca gtagcctact
    22501 attggttgat ttcgattttc cccctgcttt tggtggtggt caatatcctc ccctattttc
    22561 agattcctgt gggagaattt ctgggcttta tgaaagacgt cctgccccca agcctctatg
    22621 aaggtgtgga aaaaatagcc agagaagtct tgacgcaacc ttcaacaggt ttattgagtt
    22681 tttctgtttt atcggcgtta tggagttttt caaaatctat gaatttcttg caaaaagctt
    22741 ttaacaaggc ttatggcgtc gaaaagagtc gtggacttat ttcccatcag atgctaagtc
    22801 tcctagtcag ttttggcttg cagctcctct ttgcgtttgc cttgtttttg atcttatttg
    22861 gtcagatgat tttggcttta cttgctcatt attggacaaa agatggcatt atctatcagg
    22921 ctttacaagg gttggcaggt cctctgattt acgccctcct ctttgctatc ttggtcatgc
    22981 tttattattt tcttcccaat ctatctaaca ggaaaattag ctatacccta ccaggcagtg
    23041 cctttgttct cttagtgatt ttaggtttac taactctgtt ctctagttat ctcaattact
    23101 atgtccatca tttggtggat gtccgaattt taggttccgt tcttcttgtt gttatgatgt
    23161 tttggtttat cttgattgct aaaattgtta tcctaggcgc ggttatcaat gccagtatgc
    23221 agagtttgaa agatccggtc tttaaaaaag actaactatt tatccattat aaaagcgcat
    23281 agtatcaaga ctttatagaa ccttgatact atgcgctttt gtgctttgac agttttttgt
    23341 atagtttttg tccaagattt ctaaatcatc tgattaaatt cctattttcc cctatcattg
    23401 tccctgtttt ttcgaaaatg gaaggagtat aataaatcta tcaataaaat gataggagga
    23461 actttatggg tcatattttc ttttttctaa gtgtcttttt ggcagggatt ctatccttct
    23521 tttctccttg tatcttacct ttgttaccgg tctatacagg agtgttacta gatgataagg
    23581 atggtgctca ggcttctagc ggcaaatttt caatctcagt tactagttta ttacgaactc
    23641 tggcctttat agcaggaatt tcctttatat ttattttgtt gggctatgga gctggttttt
    23701 taggcgattt gctttatgct tcttggttcc aatatcttac tggggcaatt attatccttc
    23761 ttggtttgca ccaaatggag attctacact ttaaggggct ttataaggaa aagagactac
    23821 aactgcaagg acaggggcaa aatggtaagg gctatagtca ggcattttta ttgggcttga
    23881 cctttagttt tgcttggacg ccttgcgtgg ggccggttct ggggtctgtt ttggccttgg
    23941 cggcttcagg tggttcagga gcttggcagg gagctggtct catgttggtg tatacgctgg
    24001 gcttggcgct accattcttg cttctagctc tgacctctag ttatgttttg aaacatttcc
    24061 gaaaacttca tccctatctc ggaatcctca aaaaagtggg tggttttctc attattgtga
    24121 tgggactctt ggttctgttt ggaaatgctt caattttaag tcaattattt gaataaaatg
    24181 gaaaggaata tcaatatgaa aaaatggcaa acatgtgttc ttggagcagg ttcgctcctt
    24241 tgtttgacgg cttgttcagg caagtccgtg actagtgaac accaaacgaa agatgaaatg
    24301 aagacggagc agacagctag taaaacaagc gcagctaaag ggaaagaggt ggctgatttt
    24361 gaattgatgg gagtagatgg caagacctac cgtttatctg attacaaggg caagaaagtc
    24421 tatctcaaat tctgggcttc ttggtgttcc atctgtctgg ctagtcttcc agatacggat
    24481 gagattgcta aagaagctgg tgatgactat gtggtcttga cagtagtgtc accaggacat
    24541 aagggagagc aatctgaagc ggactttaag aattggtata agggattgga ttataaaaat
    24601 ctcccagtcc tagttgaccc atcaggcaaa cttttggaaa cttatggtgt ccgttcttac
    24661 ccaacccaag cctttataga caaagaaggc aagctggtca aaacacatcc aggattcatg
    24721 gaaaaagatg caattttgca aactttgaag gaattagcct aggaggtgtc ttatgaatga
    24781 taagttaaaa atcttcttgt tgctaggagt attttttcta gccataaccg gtttctatgt
    24841 tctattgata cgaaatgcag ggcagacaga tgcctcgcaa attgaaaagg cggcagttag
    24901 ccaaggagga aaagcagtga aaaaaacaga aattagtaaa gacgcagact tgcacgaaat
    24961 ttatctagct ggaggttgtt tctggggagt ggaggaatat ttctcacgcg ttcccggggt
    25021 gacggatgcc gtttcaggct atgcaaatgg tagaggagaa acaaccaagt acgaattgat
    25081 taaccaaaca ggtcatgcag aaaccgtcca tgtcacctat gatgccaagc aaatttctct
    25141 caaggaaatc atgcttcact atttccgcat tatcaatcca accagcaaaa ataaacaagg
    25201 aaatgatgtg gggacccagt accgtactgg tgtttattac acagatgaca aggatttgga
    25261 agtgattaac caagtctttg atgaggtggc taagaaatac gatcaacctc tagcagttga
    25321 aaaggaaaac ttgaagaatt ttgtggtggc tgaggattac catcaagact atctcaagaa
    25381 aaatccaaat ggctactgcc atatcaatgt taatcaggcg gcctatcctg tcattgatgc
    25441 cagcaaatat ccaaaaccaa gtgatgagga attgaaaaag accctgtcac ctgaggagta
    25501 tgcagttacc caggaaaatc aaacagaacg agctttctca aaccgttact gggataaatt
    25561 tgaatccggt atctatgtgg atatagcaac tggggaacct ctcttttcat caaaagacaa
    25621 atttgagtct ggttgtggct ggcctagttt tacccaaccc atcagtccag atgttgtcac
    25681 ctacaaggaa gataagtcct acaatatgac gcgtatggaa gtgcggagcc gagtaggaga
    25741 ttctcacctt gggcatgtct ttacggatgg tccacaggac aagggcggct tacgttactg
    25801 tatcaatagc ctctctatcc gctttattcc caaagaccaa atggaagaaa aaggctacgc
    25861 ttatttacta gattatgttg attaatactc ttcgaaaatc tcttcaaacc acgtcagcgt
    25921 cgccttaccg tatgtagggt tactgacttc gtcagtttta tctacaacct caaagctata
    25981 ctttgagctg acttcgtcag ttttatttac aacctcaaag ctatactttg agaaacctgt
    26041 ggctatcttc ctagtttact ttttgatttt tattgagtat aagaggtctt tcctaagcag
    26101 ttagaggaga attttgctat actgatacta gtaagtgaca aaggagagaa gcatgaccta
    26161 cacaatctta atcgtagaag atgaatatct ggtaagacaa ggtttgacta aactggtcaa
    26221 tgtagcagcc tacgatatgg aaatcatcgg tcaggctgaa aatggaaggc aggcttggga
    26281 attgatccaa aagcaggtgc cagatatcat tttaaccgat atcaacatgc ctcatctaaa
    26341 tggcatccag ttggccagtc tggtacgaga aacctatcct caggttcatt tggtcttttt
    26401 aacaggttac gatgattttg attatgcctt gtctgctgtc aaactaggtg tggacgacta
    26461 cctgctcaaa cccttttctc gtcaggatat tgaggaaatg ttggggaaaa tcaaacaaaa
    26521 actagacaag gaagagaaag aagagcagtt acaagattta ttgactaata ggtttgaagg
    26581 aaacatggcc cagaaaatcc agtctcatct ggctgatagc caatttagtt taaagtcttt
    26641 agccagtgac ttaggtttta gtccgaccta tctgagttcc ttgattaaga aagagttggg
    26701 cttgcctttt caggattatc tggtgagaga acgtgttaaa caagccaagc tcttgctttt
    26761 aactacagat ctgaagattt atgagatcgc agagaaggtt ggttttgaag atatgaacta
    26821 ttttacccaa cgttttaagc agattgcagg tgtgacacct cgtcagttta agaagggaga
    26881 agaccgatga agcgttcttc tcttttagtt agaatggtta tttccatctt tctggtcttt
    26941 ctcattctcc tagctctggt tggaactttc tactatcaat caagttcttc agccattgag
    27001 gccaccattg agggcaacag ccaaacgacc atcagccaga ctagccactt tattcagtct
    27061 tatatcaaaa aactagaaac cacctcgact ggtttgaccc agcagacgga tgttctggcc
    27121 tatgctgaga atcccagtca agacaaggtc gagggaatcc gagatttgtt tttgaccatc
    27181 ttgaagtcag ataaggactt gaaaactgtt gtgctggtga ccaaatctgg tcaggtcatt
    27241 tctacagatg acagtgtgca gatgaaaact tcctctgata tgatggctga ggattggtac
    27301 caaaaggcta ttcatcaggg agctatgcct gttttgactc cagctcgtaa atcagatagt
    27361 cagtgggtca tttctgtcac tcaagaactt gttgatgcaa agggagccaa tcttggtgtg
    27421 cttcgtttgg atatttctta tgaaactctg gaagcctatc tcaatcaact ccagttgggg
    27481 cagcagggct ttgccttcat tatcaatgaa aaccatgaat ttgtctacca tcctcaacac
    27541 acagtttata gttcgtctag caaaatggag gctatgaaac cctacatcga gacaggtcag
    27601 ggttatactc ctggtcacaa atcctacgtc agtcaagaga agattgcagg aactgattgg
    27661 acggtacttg gcgtgtcatc attggaaaag ttagaccagg ttcggagtca gctcttgtgg
    27721 accttgcttg gggccagtgt cacatctctt cttgtctgtc tctgcttagt gtggttcagt
    27781 cttaaacgct ggattgctcc tttgaaggat ttgagagaaa ccatgttgga aattgcttct
    27841 ggtgctcaaa atcttcgtgc caaggaagtt ggtgcctatg aactgagaga agtaactcgc
    27901 caatttaatg ccatgttgga tcagattgat cagttgatgg tagctattcg tagccaggaa
    27961 gaaacgaccc gtcagtacca acttcaagcc ctttcgagcc agattaatcc acatttcctc
    28021 tataacactt tggacaccat catctggatg gctgaatttc atgatagtca gcgagtggtg
    28081 caggtgacca agtccttggc aacctatttc cgcttggcgc tcaatcaagg caaggacttg
    28141 atttgtctct ctgacgaaat caatcatgtc cgccagtatc tctttatcca gaaacaacgc
    28201 tatggagata agctggaata cgaaattaat gaaaatgttg cctttgataa tttagtctta
    28261 cccaagctgg tcctacaacc ccttgtagaa aatgctcttt accatggcat taaggaaaag
    28321 gaaggtcagg gccatattaa actttctgtc cagaaacagg attcgggatt ggtcatccgt
    28381 attgaggatg atggcgttgg cttccaagat gctggtgata gtagtcaaag tcaactcaaa
    28441 cgtgggggag ttggtcttca aaatgtcgat caacggctca aacttcattt tggagccaat
    28501 taccagatga agattgattc tagaccccaa aaaggaacga aagttgaaat atatataaat
    28561 agaatagaaa ctagctaact cccagtcaat tctgggagtt ttatgcgtaa ttgggcaaac
    28621 tttctaggtt gtctatcttg ctaaaaacaa gaaaaaacgg tatgatagaa gagtgacaac
    28681 agaggtaaca gagatgaacg agacacagat tcaaagagaa acacgtcagg tagtggaaga
    28741 tgttttagaa aagaccaatt tgaaacaggg ggcccttttt gttttaggtc tatcttctag
    28801 tgaggtgcta ggtggtcaga ttgggaaaga atccagtcaa gagattggtg agctgatagt
    28861 ggagactatc ttgggtattc taggtagtag aggtatccat ttagcagttc aaggttgtga
    28921 gcatgtcaat cgggctctcg ttgttgaacg tcaggtggca gagcagtttg gtctggaaat
    28981 tgtcagtgtc catcctactc ttcatgcagg aggttcgggg cagttggcag cctttaagtt
    29041 tatgcaggat ccagttgagg ttgaatttat caaggctcat gctggattgg atatcggaga
    29101 cactgcaatt ggcatgcatg tcaagcatgt tcaggttccg attcgcccta ttttgagaga
    29161 gattggtcat gcccatgtaa cggcactggc tagtcgtcca aaattaatcg gaggtgcgcg
    29221 tgcgcactat ccgcaagatg ctattagaaa gacctgagaa tgaataaagg agataaaagt
    29281 gtttaaaaaa gaccgttttt caattcgtaa gattaaggga gttgtaggct ctgtatttct
    29341 tggaagcctt ttgatggctc cttctgtagt ggacgcagcc acctatcact atgtaaataa
    29401 agagattatt tcacaagaag ctaaagattt aattcagaca ggaaagcctg acaggaatga
    29461 agttgtatat ggtttggtgt atcaaaaaga tcagttgcct caaacaggga cagaagcatc
    29521 tgttttgaca gcttttggtt atctatcagg tgacatcctc aaaacgcttg gcttggacac
    29581 tgttttagaa gaaacctcag caaaacctgg agaggtgact gtagtcgaag ttgagactcc
    29641 tcaatcaaca acaaatcagg agcaagctag gacagaaaac caagtagtag agacagagga
    29701 agctccaaaa gaagaagcac ctaaaacaga agaaagtcca aaggaagaac caaaatcgga
    29761 gataaaacct actgacgaca cccttcctaa agtagaagag gggaaagaag attcagcaga
    29821 accagctcca gttgaagaag taggtggaga agttgagtca aaaccagagg aaaaagtagc
    29881 agttaagcca gaaagtcaac catcagacaa accaacagag gaaccaaaag ttgaacaagt
    29941 aggtgaacca gtcgagccaa gtgaagacga acaggcacca acggcaccag ttgagccaga
    30001 aaagcaacca gaagctcctg aagaagagaa ggctgtagag gaaacaccga aaccagaaga
    30061 taaaataaag ggtattggta ctaaagaacc agttgataaa agtgagttaa ataatcaaat
    30121 tgataaagct agttcagttt ctcctactga ttattctaca gcaagttaca atgatcttgg
    30181 acctgtttta gaaactgcaa aaggtgtcta tgcttcagag cctgtaaaac agcctgaggt
    30241 aaatagtgag acaaataaac ttaaaacggc tattgacgct ctaaacgttg ataaaactga
    30301 attaaacaat acgattgcag atgcaaaaac aaaggtaaaa gaacattaca gtgatagaag
    30361 ttggcaaaac ctccaaactg gggttacaga agctgaaaaa gttgcagcta atacagatgc
    30421 taaacaaagt gaagtaaatt ctgagactgc aagtcttaaa acagcaattt ctagattaaa
    30481 tacagataaa gttgaactcg aaaatcaact taagattgca cagggaaaaa ctgaaactga
    30541 tttcagcatg gaatcttgga ctgttttgag tacagctaaa aataaagctc aggaagtaaa
    30601 agataacgga acagcgaccc aagaacaaat aaacgaagca gagaaaagtt tgaaaacagc
    30661 ccttgctgac ttatctgttg acaaaacagc tttgggaagc gctatcgata cagctactaa
    30721 gaaaaataaa gagaattata caaatcaaac atgggcagag ttggaaacag cgcttacagc
    30781 agcaaaatct gtaaatacta acgagtctaa acaaagcgac gttaacgaag ctgctgaaaa
    30841 attaactgca actatggaaa aattggttga attgtctgaa aagccacgtt tgactctatc
    30901 tattgaaaag agagacatag atcgaaaagc tacggtcacg tatactttgg aaaatccagc
    30961 aaatactcaa ataaaatcaa taactgcaac tctgaaaaaa ggagaggaag ttgttaagga
    31021 ttttgtttta acagaagaaa atcttaaaac taatcattta actgctttgt ttgaaaagct
    31081 agactactac aaagaataca ctctatctac ggatatggta tataacagag gaaatgatga
    31141 tgagacggag agtatttcag aagaactaat tcagctgaat cttaagaaac tagaattgaa
    31201 agatatccag acagtctctc tgatgaaatt tgaaaatggt caagaaagtc aggttacaca
    31261 cttgtctgat aaaccgaccg atttgagtaa attatactta aaagttacat catcaacgtc
    31321 taaggatgct gtgttagccg ttagctctat agaagaagag attattgaaa ataaaaaaat
    31381 ctttaaaatt catgcagata caccagaatt agtagttcgt aagaaagatg gcagtctttc
    31441 taagggattt gactattaca tggagagagt tatccctcat gatggtgata tttattatga
    31501 ttttaaagac ttgatttcag ctatgacatc taatcctaca ggtacgttta ttttagggag
    31561 agatatttcc tcgcgtaatg taaaaccgga tggaaatgga aaatcttata tcaaaggtga
    31621 atttaaagga aaactacttg gtacaaatga taatgttaga cactcgatct ttgacttaga
    31681 atatccattg tttgatacta taaaatcagg agtcgtgaaa gatattgatt tcaagcatgt
    31741 caatatggta ttcccagata gcaatcaagg agataacgta gcaacaattg caagagtaat
    31801 caaagataag acaaaaattg agaatgttaa tgttgaaggt tatctcgagg gtcgagatca
    31861 tgtagctggt ttagttaata atcttgaagg taattccgaa attgaaaatg tatcctttac
    31921 aggaaagatt aagtctaaag gtggtaattc tattacagca ggtattgcag gtagaaatat
    31981 cttatctcgt gttaaacgtg cctatgtaaa tgcagatata gaggtacatc gatcatctaa
    32041 ttctagcatg cttgttgcag taaatggaat aaacgcagat gcaagtggtg gttggggaac
    32101 ctggggtaga cttactgagt cagttgctaa aggaacatta gaaaccaagc aaggagggca
    32161 agctggagga gcctcttcta cagtctggcc ttatggagca attgataatg ttgtcagcta
    32221 tgcaaaagtg accaaaggga aagaattatt tggatcagat ggtgatttaa attatgattg
    32281 gtttatgaaa aaaattagta atatttttgg tgttcaaggc atcagctcag gtgattcggg
    32341 tagtgattct aaatttacgc gtatttctga ggaagaagca aatcaaaaag ttgcttctta
    32401 taatattaca gctcccaact tgatgagtga ctcaagtctt ttggtagaca gactcaatga
    32461 aagttggaaa aatactgatc aatttgaaag tatacaggat tatcaagccc aaaaccaatt
    32521 aatttaccag aacttgacta aatttactcc ttattacaat aaagaattta ttgttcatga
    32581 aggaaatgct ctcactcctg aacaggaaat attgaagact aagaaaatca aatcgattgt
    32641 agggttgaaa ggaacagagt tcgttgtaga tggcagtgat attgatacta tcatgcttca
    32701 ttttgaggat ggtagtcaga agcgatataa ggtaacctct actggtaagt tttcgataac
    32761 taatttaccg gaatatcagg ttgaagattt aaatgtggtg tatacatcag aacatatagt
    32821 acatccacta gattcgtcac tgataaataa ccttgttgaa gaattgaaaa aagtagaatt
    32881 gtatacagag tctacttacc aagttctagg tattgataaa gataatgcta acaaattgaa
    32941 tagaactaag agattattct tagatgagtc attagatgct gttaaaacac aattgcctac
    33001 ctttgtaaaa acaatgtttg aaaatgagtg gttgcacatc aatggagaat ctagtggagc
    33061 tgtcgctgct ttacgacaaa aaatcatgga taataagacg gctatattgc ttgctttgac
    33121 ttatatcaat cgttattatg atgttaaatt ctcagactat aatattaaaa aactgatgct
    33181 ctttaaacca actttccatg gtgaaaagat tgatctatta gatcgattaa tccgactagg
    33241 atcatctgga gaaaatagat taaaaggttc agaaaatgca gaaacgttca agcagttatt
    33301 tgctagcgaa acgaagcaga aggatttggt gacttattta gactacaatc gctctttgtt
    33361 gactaattat caaacaacag gggaatggtt taaagaaacg actaaggatt atattcaatt
    33421 tgaggaacgt ccatccttag tagaggagat taaagatgcc aaatatcgag tttacgataa
    33481 tttaaccgca ccgtattatc aaggatatat cctacctctc ttaacgttga agaatactca
    33541 tttagcaatt ttatcgaact atagtactat gacatttgtc agccgtgaaa aacgccctaa
    33601 ttggaaaaat gaagattttg ataagtgggt gaaatatgta gcaactgctc atagaaacca
    33661 tgttgatact tggtataaga ttttacctga taatatcaaa ggtaaaatgg ttaaagagaa
    33721 tgttactgct gtttgggaag gattgagcat cccaggaagt gaatgggtag accagaatgc
    33781 tgtagataga aaaggaagag attacgcccc agctcgagaa ttctttaatc ttgtaggtgg
    33841 tcctatggga ggttggtatg cttaccatgg ttatggtgcg catgctggag gaagaaatcg
    33901 ggttaattat gaggtgtttg atgtattaag tgagtatggt atatcggtct ttactcatga
    33961 attgactcac gttaatgata cttggattta cttaggaggg tatggtagac gtgaaaatat
    34021 gggacctgaa gcttatgctc agggattatt ccagtctcct gttccaggtc agccgggttg
    34081 gggggcgctt ggtctcaata tggcatttga aagaaaaaat gatggtgatc taatttataa
    34141 tgcctcccca actcaatttg aaaataggaa agagttagat agttatatga aaaattataa
    34201 cgatactctc atgatggtgg actacttgga aggagatgca gttatctcta aaggtaaaga
    34261 agctattact aaatggttta aaaaagttga acctaaggtt gttagtcaaa cagctcagta
    34321 tgatacggtt cgtcagttga ctgctgaaga gaaagaaaaa ctttctgttt ctagtgttga
    34381 tgatttagtt gatcaaggat taatgtcaga cagagctgta ggaaataata cctataatcc
    34441 agctgatttt gaaacaagtt atattgcgat agattatatg actggtattt atggtggagg
    34501 aaagaatagt gtaggttcac caggtgcctt gatgtttaaa cataatactt tccgtatgtg
    34561 gggctactat ggttttgaag aaggtgttct aggatatgct tctaataaat ttaagcaagc
    34621 atctagagat gaaggtcatg ctggacttag tgataacttt attatttcta agatttccaa
    34681 aggggaattt ttaactatgg aagcatttaa aaagggatat tttaagaaag tagtagaaga
    34741 gttaaaaact aaaggtattc gtcctgttac tatcaatcaa aaaacatata gcacctttga
    34801 agaattgcaa gaaggattta agcaggctgt tgaaagagat ttgaagaaaa atcagttaga
    34861 tgagagggaa acaagaaact tcaaattcca agttttcaga caacttcttc aacaaacaga
    34921 tagctttaaa acttctatct ttagataatc gaaatctctc atctgctttg cggatgaggg
    34981 ttttcttatt ttatgctata cttaaagtat gcatagaaaa acagtgattg attttaggac
    35041 tttgggggag agatatacct ttacccagcc tattaaagag ttgaaaacga gaaatgtaga
    35101 agaagtggca gatttgctgg cacaagtgga aagctaccaa gagcaaggtt attatgtggt
    35161 ggggtatgtc agctacgagg ctgcacctgc ttttgaggaa aaattagcag ttcacaagga
    35221 tcccctactg ggagagtacc tgctttattt tactgttcat gatagcgtag aaacttcccc
    35281 tattcctctg acttatgagg atattgattt gccctcaaat tggcaggaag taacgtctgc
    35341 agcagactat gaaaaggcca ttgcccaaat tcaccatcat ttacggcagg gagataccta
    35401 tcaggtcaac tacaccgtcc aacttaagca aaagttaaat gccaatcctt ttgccatcta
    35461 caatcgtatg gtggtagagc aggaggcggg ctacaatgcc tatgtggaac atgacgagat
    35521 ggcagtgatt tccatgagcc cagagctctt ttttgagcaa aatgatcgcg agttgacaac
    35581 acgaccaatg aaggggacga ctcagcgtgg ggtaactgac caagaagatc ttgaacaggc
    35641 cagttggttg gaacaggatc ccaaaaatcg ctctgaaaat atgatgattg tggacctctt
    35701 gcgcaatgat atgaaccgta tttctgaagt tgggagcgag cacgtggagc gtctgtgtca
    35761 ggtagagcag tattcaactg tttggcagat gacttcgacc atcaagagtc agttgcgaga
    35821 ggatgtggac cttgttgaaa tcttccactc actctttcct tgtggttcca taacgggtgc
    35881 accgaaaatt gcgacaatgg agattatcaa ggacttggag cctcaaccgc gtggagtcta
    35941 ctgtggaacg attggtctct tgcttccaaa tggacgacgg atttttaatg tggccattcg
    36001 taccattcaa cttcacaaag gtcaagccat ttatggagtt ggcggaggga ttacttggga
    36061 tagcacttgg gaatctgaat accgtgaagt tcatcaaaag gcagcagttc tctatcgtaa
    36121 acaagctcgc ttccaattaa ttacaactgg aaaaatcagc caaaaacaac tgatgtttga
    36181 agaacaacat ctggaaaggc tgagaaaagc tagtcggtat tttgcttatc cttttgaccc
    36241 agaaattttg agacaaaaga ttgaagcaga gtgtcagact tgtgatgcta atcaagatta
    36301 ccgcttgcga atttctctca gcaagtctgg agagatagaa ttcagtcgtc aaatcttaac
    36361 acctcttagt ccaagttttt gtcaggccaa actttgtctg caagaagcga atttgaatca
    36421 atcctttacc tattttaaaa cgacttacag accgcatttg agcatagagg aacaagagaa
    36481 gatttaccat aatggaaaag gagaacttct tgaaacctct atcggaaatt tggttctgaa
    36541 aatcgatggg aaactctaca caccgcctat ccgacttgga atcttgccag gaatttatca
    36601 ccagtattta ctagaaacag gacaggtaga agagaaagtc ttgaccttgg cagattttgc
    36661 ccaagcagaa gctatttacg gctgtaatgc agtgagaggc ttgtatgagt tgcaagtgat
    36721 aggattgtga gttttagcta ttaatagttt ttaatatata ttaaaatata ttggtgttta
    36781 cttttgatga gatatcatat ttgtgttata atgtaaccgt aaacaataga aatttaaagg
    36841 agatttttga tgaaatcaaa aatagttttg ggagcttcgc tagcgatcgc aacacttagc
    36901 ttagtatctc ttgtcgagat tgaaggactc tcaccgtttt taatagagaa tgttagcgca
    36961 aatactcatt ctgcaaataa agttattaat cataaagtaa gtatttactt agaaaatgct
    37021 gatgaaggta aaggtctaac tgtaaacttt tcaacagaca gtgttagtcc aaatttattt
    37081 gatgagttcg aaaaaaagag tggaattaca ataactacta tgttagtgaa tgctaagaca
    37141 ggtgaagttg tagaaaaaag attaacacca agcgtttttt tacgttccaa tgatttaacc
    37201 agtgggacca tatcttcgtt tatttttagt gaatatcctg atggagaata taaatatgtt
    37261 gtgtctaaag gagactttat cgatcctaag actcagttta aacatcagta tagaggagaa
    37321 tctccagttt ttagaatacg taatcgaaaa tatgtggagt tgggaacaac tgataaaaaa
    37381 ttagatgaaa gacgtgataa tagcgtttat aaagatgggg ttgttgaaca caaggtaaat
    37441 ttaagtttaa caagttatca aggaggtaat ggagttactg caattttttc aactgatagt
    37501 gttaactcta acttacttaa ttcttttggt gaaaaggcta aaaaagtttt gattaggtct
    37561 aagttaatta atgttaagac tggcgaggtt attgatgaaa cgttttctcc aaaagtaagt
    37621 ttaacctcta aaattttgaa aagtggttct acagcagtat tttattttat agatttaact
    37681 gatggcgaat ataaatatgt tgcgtatgaa agccagcaat atactgatcc tcaaacaaca
    37741 ttgactcatc agtatagagg agaatctcct atttttagta tcaaagatgg aaaattttct
    37801 ggtttagtat ctgcctcaaa accagacgaa aacccgaagc caacgccaaa accggatgag
    37861 aaaccaaaac cttcagcacc acaacaggag aaaacaaaac ctacagttca gtctggttgg
    37921 gtaggttcat cttattatca aaatggtaaa aaagtcacta gtaagtggat ttttgataaa
    37981 aaatataatt catacttcta tctagatgca tcaggaaatt atgttcaaaa cgcttgggta
    38041 ggtaactatt atctcaaatc aggcggatac atggctaaaa gtgagtggat ttatgataag
    38101 aactatggtt catactacta tctaacatca gaaggcagct acgctcgtaa cacttggtca
    38161 ggcaattatt atttgaagtc aaatggtaag atggctaaaa gtgagtgggt atacgatagt
    38221 aactacaaat catattacta cttgacatca gaaggaagtt atgctcgtaa cacttgggta
    38281 ggcaattact atcttaaatc aaacggtaaa atggctgtta atgaacgtac accagatggt
    38341 tatcgtgtag atggttcagg taaatgggtt aaataagaaa ctaaaaaata ggataatagg
    38401 taatatcaga tgaataaaaa aaattatttt catgtgcttt agcattttca acgattggaa
    38461 cattgtcaac gattgataaa tcaggagttc ttcctttttc agcgcaaacg gtgcaggctg
    38521 acatacaaac gcttacccca atggcaaaat attatattat tcttccgaag gatgcagaaa
    38581 tatataaaac ttggagaggt actgtcaata ttcccattat tgatgctaca aaaactactc
    38641 cagaattatc ttatttcaaa gaagatcatc gcaactatat agctaatgag aataagtctg
    38701 gtgccaatta catagagtgg aagggaacag tagaagagtt taaagaggcc attaagaagt
    38761 tgactgataa aaaatcaact actgcaacac caaaaaagga tgaaaagcca acaccaaaac
    38821 cggatgagaa gccaaaacca acacctacag ttcagtctgg ttgggtaggt tcatcctatt
    38881 atcaagatgg taaaaaagtc attagtaagt ggatttttga taaaaaatat aattcatact
    38941 tctatctaga tgcatcagga aattatgttc aaaacgcttg ggtaggtaac tattatctta
    39001 aatcaggcgg atacatggct aaaggtgaat gggtttatga tgccacctat caagcttggt
    39061 attatttgac atcagatggt tcttatgctt acagtacatg gcaaggaaat tactatctaa
    39121 aatcggatgg taaaatggct gtcaatgaat gggttgatgg tggacgttat tatgttggcg
    39181 ctgacggagt ttggaaggaa ggtcaagcaa gtacagcttc ttctagtaat gatagcaata
    39241 gtgaatattc tgctgcttta ggaaaggcaa aaagttataa ttcgttattc cacatgtcaa
    39301 aaaaaaacgt atgtatagac aattaacttc tgattttgat aaattttcaa atgatgcagc
    39361 tcaatatgcc attgatcatt agatgattaa cagatactgg taggcgaaaa aattccttat
    39421 aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc
    39481 attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat tattgggatt
    39541 gaccttggtg gaacttctat caaatttgca atcttaacaa cagcaggaga aatccaagga
    39601 aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt
    39661 gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt
    39721 ggaatgggat caccaggtgt ggttgaccgt gaaaaaggga ctgttatcgg tgcctacaac
    39781 ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcctt gggcattcca
    39841 tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctgga
    39901 gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg cggtatcgtc
    39961 gcagaaggca aattgcttca cggtgttgct ggtgcagcag gtgagttggg ccacatcact
    40021 gttgactttg accagccaat tgcatgtact tgcggtaaga aaggctgcct tgagacagtt
    40081 gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat
    40141 gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac tgtctttgag
    40201 ctcgcaaaag aagaagacga ccttgctttg attgtttacc gtaacttctc acgttacttg
    40261 ggtattgcat gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt
    40321 ggtgtgtcag ctgcaggaga attccttcta caaggtgttc aaaaagtcta cgatgaaaat
    40381 agtttcccac aagtacgcac atctactaaa ttagcacttg caactctagg aaatgacgct
    40441 ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa
    40501 cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg
    40561 aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta
    40621 aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat
    40681 tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc
    40741 acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa
    40801 gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta ctggaatgac
    40861 tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt taagaaacac
    40921 gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg ccgcaatatt
    40981 atttcgctct gggattacca agctttcgaa gaaacagatg ggctgctccc gtgcgccttt
    41041 cagaccatgt ttgatgtccg gcgtgttgat ggggaaatct atctggatgc gaccttgacc
    41101 cagcgctcca atgatatgct ggtggcccac cacatcaacg ctatgcagta tgtggctttg
    41161 cagatgatga ttgccaaaca ttttggctgg aaggttggga agttcttcta cttcatcaac
    41221 aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg tcgggagccg
    41281 tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt ctttgatatc
    41341 aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt gaagtttgac
    41401 ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct
    41461 taacgtgata cgcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt
    41521 tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg
    41581 acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct
    41641 cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt
    41701 tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa
    41761 aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg
    41821 caaagcgctt taatggcgaa gtggttagtg gagatagcca gcaagtctat cgaggacttg
    41881 atattgggac ggccaaggct agtccagaag agcaggcagc tgttcctcat catttaatcg
    41941 atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa gctaagatga
    42001 ctattgagga tattcagagc cgtggcaagc tagccattat cgccggtggg actggacttt
    42061 atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt
    42121 tagcttatcg agctagtttg gagccatatt cagatgagga attagcccat ttggtggagc
    42181 aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg
    42241 cccattttgg tcaggatttg gaaaatcaag agatcttgta tgaaccgctg attatctgct
    42301 tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg
    42361 aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg
    42421 ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag acctttgagg
    42481 aagctcgtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt
    42541 tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta caagaccgca
    42601 ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc
    42661 ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg
    42721 gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa
    42781 aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg
    42841 gatgcagaag aaatcactac tgtcatcgtc aacaatcgtc tgaccccaag gcagaatgtc
    42901 aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat tttggatatc
    42961 tttgccatgc gggctcgaag ccatgaaggg aagctccaag tccacctagc ccaactcaaa
    43021 tacctcttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt
    43081 ggttcccgtg gtcctggtga aagccaactg gagctgaacc gtcgtagcgt tcgcaatcaa
    43141 atcacggata tcgagcgcca gcttaaggtg gttgagaaaa atcgtgcgac tgtcagagaa
    43201 aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttatactaa tgctgggaaa
    43261 tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg
    43321 actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt aactttgaca
    43381 gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc
    43441 ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac
    43501 cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt
    43561 cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg
    43621 ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg
    43681 ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc
    43741 tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac
    43801 ggcgaagtga ttacaggcta catttccgag aaaaataaat ggaggttaga agaattttat
    43861 gactgatatt aaaactttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta
    43921 tctggatcag cttctagctg gtaaaacaga tcaggagcag ttaacactta ttacacctcc
    43981 gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc ctcaggctgc
    44041 gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt
    44101 caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg
    44161 ttatgagagt gacggccttg gtcggatttt ctttgaaaat gaggagaaaa tctcggatga
    44221 cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag agtctggtaa
    44281 gatttacatg aagcctgtcg aggacgagga agttatcagc ctagaaagtc tcacagcttt
    44341 gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga
    44401 tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac
    44461 agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg
    44521 ggggctggtc agccctctaa agcccgcaac gtttcaagtc tcgccctgaa acttttggat
    44581 gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa
    44641 accacaattc gaccacgtaa ggtcagcaaa atctttatta cccatctgca tggagaccac
    44701 atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca
    44761 gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg
    44821 tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctgggt
    44881 aaaattcttg aaaccgataa attcactgtg tatgcagagg agctggacca cactattttc
    44941 tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc tgaaaaactc
    45001 aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt
    45061 gttttggaag acggaactga aatcaaggca gcagactata tctcagcgcc acgtccaggt
    45121 aagattatca ctattttagg agacactcga aaaacgggtg ccagtgtgcg tctggctgtc
    45181 aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt
    45241 aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa
    45301 cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag caaactcaag
    45361 aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa
    45421 atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg
    45481 gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa
    45541 ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg
    45601 atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca
    45661 agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt gaccagattg
    45721 ctgataaaga tattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc
    45781 gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca
    45841 tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta
    45901 ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag
    45961 tcaatccagg tccaatccga acaggatttt tcgaccaagc cgacccagat ggaacttatc
    46021 ttaaatcggt tgaccgcttc ctgctagaac cagatgcagt ggctaaaaag attgtcaaga
    46081 ttataggaaa aaacaaacga gaactcaatc tcccgatttt gttgaaccta gctcataagt
    46141 tttataccct ctttcctaag ctagctgata agttggcagg ggaaactttt aattataagt
    46201 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
    46261 tatcaaactg aacttcttct aggagatagt ctataaagcg ttcgcccatc ttagataggc
    46321 tggttttctc atgctggata tagaccagct cgatcgggtc atcaatatcc agtgggatag
    46381 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
    46441 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
    46501 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
    46561 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
    46621 ggttggtctt gctgacaaag atatgtggtt gcgctgtgaa gagatggtgg gctagcaggt
    46681 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
    46741 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
    46801 ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
    46861 caaaggcata gtgttgagac gaaacgctaa atagttcgcg gtgggcgaca ggatttttat
    46921 agcgttcctc cagaagctgg gtctgctcga caacctgacg ggcataagag agaaactcca
    46981 tcccatcacg ggtcaaggtg attcccttgg gattgcggat aaagatctca atgcccattt
    47041 cattttccaa atctcgcact gcattggaga gacttggctg agtgataaag agttgcttgg
    47101 ctgcctcatt catggagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
    47161 tcatgttttt attgtaccat acttgcctta gaatcgctaa agaagatagg aaaataagga
    47221 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
    47281 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
    47341 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
    47401 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
    47461 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
    47521 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg ccgtgcggct
    47581 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
    47641 cgcgacccag cagaattcca cagaaaacat atccttggtg cacgcaatat tccttcaagt
    47701 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
    47761 caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag
    47821 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
    47881 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
    47941 ataaaattta aggagttctt agaacataaa agaaaagaag aaaaatccac tttttgtggg
    48001 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat
    48061 aggattggtc ttggctattt cataccaaaa agagtcataa ttagactata aaatagaaaa
    48121 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
    48181 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac
    48241 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
    48301 tttctgctaa atctgggttc aatgtgaagt ccccaaattg gattctcttg aggatagtca
    48361 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
    48421 tgatagaggc ttgactgatg gagggacttg cagatagaat ctctagtttt gcgggtttac
    48481 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
    48541 gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt
    48601 ggaagcccaa gggaccgtta tcggtcaaga ggaggagtcc cgttgtatct cggtccagtc
    48661 ggccaacggc atagagcttg tcagactgga tgtcagatgg aagcaggtcc atgacggtcg
    48721 gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg
    48781 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
    48841 tattttgagc tagggagcgg gctgggcaac catcgactag aattttccct ctgaggagtg
    48901 cttgcttcat ggcctttcgg ctaacttttt cttgggctaa taaattatct aaacgcatac
    48961 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
    49021 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
    49081 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
    49141 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
    49201 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
    49261 tcaaacgata tcgaaaaaga gcgtggaatc accatccttg ctaaaaatac tgccgttgct
    49321 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt cggtggagaa
    49381 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
    49441 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
    49501 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
    49561 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
    49621 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
    49681 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
    49741 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
    49801 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
    49861 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
    49921 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
    49981 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
    50041 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
    50101 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
    50161 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
    50221 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
    50281 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
    50341 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
    50401 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
    50461 gttccagcgc gtggtttgat tggatactca actgagttct tgtcaatgac tcgtggttac
    50521 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
    50581 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
    50641 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
    50701 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
    50761 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
    50821 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
    50881 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
    50941 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
    51001 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggactgtcaa
    51061 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
    51121 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta
    51181 ctttagtgtg agagacatca cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
    51241 agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct ttttagttaa
    51301 caccatctct tttacttgta tagtatgaat gggaaaaact tgaataaaat tcgttaatgg
    51361 catctatttt atatttggtt agtactggtt gcatattatt ttgaaataaa actactatat
    51421 aatattattc atagagataa tatagtaaaa catctaaaaa attcgatttt ttccttgaca
    51481 acaaataata aaaatgttat tcttatcaca taagaaagcg atttcaacaa aagagagagg
    51541 aggatttatt ttgttgcatt gttgctcaag gtgtgatttt tttgaaagaa aaaataaaat
    51601 gaaaacgaaa aaacatagat tacttgctct agctcttatt tcaaatttta cattattggg
    51661 agctgcatca gctgctgtac aatatccaga tggaggagta tggacatatg gagaaggttc
    51721 aggaggtggt taggcttttt caaattacta tcatggtaaa aaatatcatt attcatccat
    51781 cgtgagcaga tgggatggtc attctgataa aggagaagct cctgctggaa aaacctccta
    51841 tgcatggatt tggactaaat ggggagaaca agtagcattt tactatgact atgactaata
    51901 tttagttttc ttagataatt ccccttgtaa gaaattacag ggggaatttt gtttacaagg
    51961 agtctactat gaagaaacta ttcatattat tatcaacttt ttttctcagc ttcttccttg
    52021 cttggattat tgtcttacgt gcgccacaat atttatatgc aagctatgat tccgtttctt
    52081 tacttcgtgt caaaaaagat actcaggaac cgacgcgtga ggtatttgaa caggaattgg
    52141 agaattttgc aaactcagaa cagagtttaa tagctagaag aattgtagag ccgagtaagg
    52201 atgggacgac tcactttact tatgcaactt atggtcaggg aactttacca aaagaattcc
    52261 aagaagctag ccaagaaagt cgtgaacgta gtgatccgct aaatagttat ctccttttgt
    52321 caggctcctt gacgaaagaa aagcttgccg ataaattagg agatttgggt tataaagcaa
    52381 gtgctgaccg aaagataccg tcctattttc ttgcttttcg aatattacta aatcccctta
    52441 ttttaattag tttagcaata tttggcttat ctttctttgc tttagtgatt atcactcgga
    52501 ttaaggaaat gagagcagca ggtataaaac tcttttctgg tcagactctc ttatccatca
    52561 tggggcattc tttatcgact gatatcaaat ggctccttct atcagccctc ctttccttcc
    52621 taggtggggg agtcgttctt tttagtcaag gtttgtttta tcctatcttg ttagccacct
    52681 atggttttgg gattagtttc tatctgttgt ttttattggc gatttcaatt ttactaatgc
    52741 ttctttatct aatgagtttg aattacaaag cattagttcc cgttattaag gggagattac
    52801 cccttaaacg cctgatgatt ttaaccctat tgtgtcagtt agtagctgtt tttacagtag
    52861 gctacgctgt taagacaggt ttgacgtctt accaacgatt gaaagaactt gaaatttcaa
    52921 aacaagcatg gcaggataga gcagactatt atcaaatttc ttttggctta ggtgatagag
    52981 gaaaagatac agaaaatcag agcaagtggt atgcctttgc caaggaagca atcgaagaag
    53041 aacaagctct ttatgtaaag gataatctgc tccattttgc caatccacaa ggaaaaaatg
    53101 aacagggaga gacactggat acctatagtc cagatgctaa tacgctctat gttagtccca
    53161 gttatttgga caaggaaaag gtcgtggtag atgctgagac caaacagaag ctagcccatc
    53221 tccaaaaagg tgagtttatc ctcttgctcc cagaacattt gcgctctcga gaagcagaac
    53281 ttaagaaagt ttttgaagaa agattgagtt attatggaaa atctggtgag gaggcaagtg
    53341 ctcctttgga ttatgagatg aaagcgcacg ttagttatct ttcaatggga gaaaagcggt
    53401 ttgtttataa taacggtgag aatcccgtat ccactcagta tttgactgat ccgattttag
    53461 tggtattcac gccgacttct acaggtgata gttttatatc cttatctagt tggtctatca
    53521 atgctggaaa acaactcttt atcaaaggat atgagagtgg gctagaactc ttgaagaaag
    53581 ctggaattta tgagcaagta tcctatctta aagaaggaag aagtgtttat ctaactcgtt
    53641 ataatgaagt tcaaactgaa acagcaactt taatcttagg agctattgtg gggatagcta
    53701 gttccttgtt actattttat tctgtcaatc ttctatattt cgagcaattc cgccgagata
    53761 ttttgattaa acgaatttca ggtttacgat tttttgaaac acatgctcag tatatggtta
    53821 gccaatttgc cagttttgta tttggtgcta gtttctttat tttacgcagt agagactggg
    53881 taattggctt actcacttta ttagtctttc tagctagtgc agttttgacg ctttaccgtc
    53941 aagcacagaa agaatctcgt gtttctatga caattatgaa aggaaaatag gatgattgaa
    54001 ctaaagaata tatctaaaaa atttggaagc cgtcagctat tttcagatac gaatcttcat
    54061 tttgaaggtg ggaaaattta tgccttaatc ggtacaagtg gctgtggtaa gacaacactc
    54121 ttgaatatga ttggacgatt agagccatat gacaaagggc aaatcatcta tgatggtact
    54181 tctcttaagg acatcaagcc ttctgttttc tttagagatt acttaggata tttatttcaa
    54241 gattttggct taattgaaag ccaaactgtc aaagagaatc tcgatctggg tttagttggt
    54301 aaaaagttga aggaaaaaga gaaaatctct ttgatgaaac aagctctaaa tcgtgtaaac
    54361 ctctcttatt tggatttgaa gcaacctata tttgagttat caggaggaga agcacaacgt
    54421 gttgcactag cgaagataat tttaaaggat ccacctttga tcctcgcaga tgaaccaacc
    54481 gcttccttag accctaaaaa ctctgaggaa ttactttcca tcctagaatc tttaaaaaat
    54541 ccgaatcgga ccattattat tgcgacccac aatcctctga tttgggggca agtggatcag
    54601 gtcattcgag ttaccgattt atcacataga tgatatggta agattcagtt agaagaaaga
    54661 gtcacaaaca cactttgtgg cttttttatt tccataaaaa tggtaaaata gtaggagtag
    54721 aaatggagtt cgagacatga aagtaataga tcaatttaaa aataagaaag ttcttgtttt
    54781 aggtttggcc aagtctggtg aatctgcggc tcgtttgttg gacaagttag gtgccattgt
    54841 gacagtaaat gatgggaaac cattcgagga caatccagct gcccaatgtt tgctggaaga
    54901 agggatcaag gtcattacag gtggccatcc tttggaactc ttggatgaag agtttgccct
    54961 catggtaaaa aatccaggta tcccctacag caatcccatg attgaaaagg ctttggccaa
    55021 gggaattcca gtcttgactg aggtggaatt ggcttatttg atttcagaag caccgattat
    55081 tggtatcaca ggatcgaacg gtaagacaac cacaacgact atgattgggg aagttttgac
    55141 tgctgctggg caacatggtc ttttatcagg gaatatcggc tatcctgcca gtcaggttgc
    55201 tcaaatagca tcagataagg acacgcttgt tatggaactt tcttctttcc aactcatggg
    55261 tgttcaagaa ttccatccag agattgcggt tattaccaac ctcatgccaa ctcatatcga
    55321 ctaccatggg tcattttcgg aatatgtagc agccaagtgg aatatccaga acaagatgac
    55381 agcagctgat ttccttgtct tgaactttaa tcaagacttg gcaaaagact tgacttccaa
    55441 gacagaagcc actgttgtac cattttcaac acttgaaaag gttgatggag cttatctgga
    55501 agatggtcaa ctctacttcc gtggtgaagt agtcatggca gcgaatgaaa tcggtgttcc
    55561 aggtagccac aatgtggaaa atgcccttgc gactattgct gtagccaagc ttcgtggtgt
    55621 ggacaatcaa accatcaagg aaactctttc agccttcggt ggtgtcaaac accgtctcca
    55681 gtttgtggat gacatcaagg gtgttaaatt ctataacgac agtaaatcaa ctaatatctt
    55741 ggctactcaa aaagccttgt caggatttga caacagcaag gtcgtcttga ttgcaggtgg
    55801 tttggaccgt ggcaatgagt ttgacgaatt ggtgccagac attactggac tcaagaagat
    55861 ggtcatcctg ggtcaatctg cagaacgtgt caaacgggca gcagacaagg ctggtgtcgc
    55921 ttatgtggag gcgacagata ttgcagatgc gacccgcaag gcctatgaac ttgcgactca
    55981 aggagatgtg gttcttctta gtcctgccaa tgccagctgg gatatgtatg ctaactttga
    56041 agtacgtggc gacctcttta tcgacacagt agcggagtta aaagaataat atgaaaaaaa
    56101 ttgtctttac aggtgggggg acggttggac acgttaccct caatcttttg ttaatgccta
    56161 agttcatcga agatggttgg gaagtccact atatcgggga caagcgtggt atcgaacacc
    56221 aagaaatcct taagtcaggt ttggatgtcc ccttccattc tattgcgact ggaaaattgc
    56281 gtcgctattt ctcttggcaa aatatgctgg atgtcttcaa agttggttgg ggaattgtcc
    56341 aatcgctctt tatcatgttg cgactgcgtc cacaggctct tttttcaaag ggaggatttg
    56401 tctcagtacc gcctgttatc gctgcgtgtg tgtcaggagt gcctgtcttt attcacgaat
    56461 ctgacctgtc tatgggcttg gccaataaaa tcgcctataa atttgcgact aagatgtatt
    56521 caacctttga gcaagcttcg agtttgtcta aggttgagca tgtgggagca gtgaccaagg
    56581 tttcggatca aaaaaatcca gaaccagatg aattggtgga tattcaaagc cactttaatc
    56641 ataaattgcc gactgtattg tttgttggcg gttctgcagg tgctcgtgtc tttaaccaat
    56701 tggtgacaga ccataagaaa gaactaacag agcgctacaa tattatcaat ctaactggag
    56761 attctagcct gaatgagttg agccaaaatc tttttcgtgt tgactatgtg accgatctct
    56821 atcaaccctt gatggaattg gctgatattg ttgtgacacg tggtggtgcc aatacgattt
    56881 ttgagctctt ggcgatagca aaattgcatg tcattgtgcc acttggtcgt gaagctagtc
    56941 gtggtgacca gattgaaaat gcagcttact ttgttaagaa aggctatgca gaagaccttc
    57001 aagaaagcga tttgaccttg gatagtttgg aagagaagct ttctcactta ctaagtcaca
    57061 aggaagatta ccaagctaag atgaaagctt ctaaggaatt gaaatctcta gcagattttt
    57121 atcaattgtt gaaaaaagat ttatcataag gaaagtaaat gtcaaaagat aagaaaaatg
    57181 aggacaaaga aatcctcgaa gaattgaaag agttatcaga atggcagaaa cgaaaccaag
    57241 aatatctaaa aaagaaggct gaagaagaag cggctctagc tgaggagaag gaaaaggaaa
    57301 gacaagctcg aatgggagaa gaatctgaga agtcagagga caaacaggac caggagagtg
    57361 aaacagacca ggaagattca gaatcagcta aggaagagtc tgaagaaaaa gtagcatcct
    57421 cagaggctga caaagagaaa gaagagaaag aagaaccaga gtctaaagag aaggaggaac
    57481 aggataaaaa gcttgctaaa aaggctacaa aggaaaaacc agccaaagca aagattcctg
    57541 gtatccatat cttgcgagcc ttcacgattt tatttccaag tctgctttta ttgattgtct
    57601 ctgcctactt gctcagtcct tatgcgacca tgaaagatat tcgtgttgag ggaacggtgc
    57661 aaactacagc tgatgatatt cgacaggctt caggcattca ggattcggat tatacgatta
    57721 accttctgct agacaaggca aaatatgaaa agcagattaa gtctaactat tgggttgaat
    57781 cagctcaact tgtctatcaa tttccaacta agttcactat taaggtcaag gaatatgata
    57841 ttgtggccta ctatatttct ggtgaaaatc attatcctat tctttccagt ggtcagcttg
    57901 agactagttc tgtgagtctg aacagtttac cagaaactta tatatcagtt ctctttaatg
    57961 acagcgaaca aatcaaagcc tttacgtcag aacttgctca aattaaccca gaactcaagg
    58021 tggctatcca aaaggtggaa ttagccccaa gcaaggtgac ttccgattta attcgattga
    58081 ctatgaatga ttcggacgaa gtcttggttc cgctttctga aatgagtaag aaattgcctt
    58141 attacagtaa gattaagcct caattgtcag aaccaagtgt ggtcgatatg gaagctggag
    58201 tctacagcta cactgtagcg gataaattga ttatggaagc agaagaaaaa gctaaaaaag
    58261 aggccgaaga agctgagaaa aggcaggaag aggaacgtaa acgtttagaa gaagaacaga
    58321 aaaaacaaga ggaagagagc aatcgaaacc aaacgaccca gcgctcatcg cgtcgctagg
    58381 ttttaccttt tctcctatag ctctttagtt gccatgtttt tacgtttaat atttgacatc
    58441 tgtttttctt tatgttatat ttgcaatgta atcgattaca aaaaaatttt ttatgaagaa
    58501 ggtaacacat atgtcagatg aagtattctt atttgaagaa attgaagaaa tcgtagcccc
    58561 tacagatggt gaattttggg ggggagtttt acttggaact ggggtagttc tcttaattgg
    58621 agtagcctgt tgttaaaaag atagggagtg ataatcatgc aagataactt tttatttgag
    58681 gaaattgaag aaatttcagt accagttagt gatttttcag ctggacttgc aacaggtatc
    58741 ggattaggtt tagcaatcct tgctcttgct ggttgttgaa gtttgttcac ttactaatat
    58801 caagcttttt caatttcatt ttagacagtc atttgaattt tccgtattag tcttgcagca
    58861 agagattaat agaattagtc attattttat tgattgcgga ctgagggact agagtatgtt
    58921 ttacttagcc cctcttttat ttattaaagg ttaggtttgt tatgagaatt gttgataaga
    58981 ttaagatact acctactcct tatgagggac agtatcatct atatatacca tccagtaaga
    59041 aacatgtatt agttgggaaa caggaaaaaa aatggttaga gcaactaata ggtcaagaat
    59101 ttaccatatc ggacttatta gtgttagtag ggaagaaata ttatttaaga ttttatattg
    59161 ttcttagtaa aatgggactt atagattcta tgaaatcacg aaaaaagata aatattttta
    59221 aaatatcttg ggactttaat ataacattat ctgaaaaatt aaactataaa agatttaata
    59281 agaattttga aaaaatccta tttggtattt tcccattggc tcttattttg caatgtttta
    59341 ttatgtatat ttatagggcg gatattttga ctaatttcca aaactttcca ataacgctaa
    59401 acattctttt attttggttg cttgttgtta ttatatctgc aacgatacat gaatttagtc
    59461 atgcaataat tgctaataat tatcaaattg agatcccaaa ttgtggaata atgctacttt
    59521 tgtttaattt ggcttttttt gtagatttaa gtggggtgca attcctaaaa aataaaaaac
    59581 aatttttgaa aattatgtta gcaggaattg cttcaaattc gattttatca cttacaggtt
    59641 tacttgtttt attgttcaca tcgtataaat tgcttggact cttatttttt atcattaact
    59701 taggtatgat ttttattaat tcaattcctt tttttcagta tgatagtggt attattttaa
    59761 gatacttgaa ttctaacaat aataacttga attttcaata tatagttcaa cttttaatag
    59821 catttgttat tatttatttc cctttgagtc aactattaca atttttgaca cctaatatta
    59881 ttgttcgtag tataggaggg gtagttgttt ctatactgct ttctatatta tatatgagag
    59941 gaaggacgaa atatgttcta cgtaaatagt tatgtttttg cttataaaaa agaaggtata
    60001 atgtatttgc gtggtcggag tatgcgggaa atagctatag aacctcaaat ttcgcaagaa
    60061 tttatcaacg atctatttaa tagttgtaag gaactattag agatagaaga agtattaggt
    60121 agtaaactaa catttgaact attaaatgaa caaattttaa tttcggatga gatagatatt
    60181 gatagtagat attctagaac taaaggttac tattcgttat tttataatga agagtataat
    60241 aaaatacaga ataaaacagt attagtatta ggagccggag ccttaggatg ttatatatct
    60301 ctaagtctaa gtatgtatgg agtgaggaaa cttattgtcg ctgattacga tataatagaa
    60361 ccatcaaatt taaataggca aattctttat acagagtcgg atgttggtaa ggagaagatt
    60421 aatgttcttt ctcaaaaaat acacaagtat aattcagatg ttcaggtagt acctatttct
    60481 attaaagttt cttcagtaga agaattagaa aacattgttg cggaatatgg gagtatagat
    60541 tttatcgtta aagcaattga tacgcccatt gatattataa aaattgtcaa tcaatttgct
    60601 gtatcgcata agatatccta catatcagga gggtttaatg gatgctatct tattattgat
    60661 aatatatata tccctaccat cggttcttgc tttgcttgtc ggaatataaa caaagatata
    60721 aataagtaca ctttatctga taagacaaag tggccgacta caccagagat gcctgctatt
    60781 ttgggaggga taatgactaa tttaataatt aaaatatttc tgggatgtta taatgaaatc
    60841 ctaatagata acgcttacgt ttataatatg agaaatcatg ctctaagtca agaaaaatat
    60901 gttctggaaa acggagaatg tccaatttgt aaaaaaaata ataaagtgaa agataacaat
    60961 attagagcga aaacatttat tcgttcagtt tgtttttgct tattatcagg aggagtagct
    61021 tttttatctg ctattgggca gttcactgtt atagaaacac aattaatagt attgttcttg
    61081 ggtattattt ttgctatata ttatgcttac tacaataaaa atattcaaac atcattggaa
    61141 aatatagtat ggcttttttc atcgtttgag attttatttt tgcttgttaa ttttagaaca
    61201 tttattcagt taccagtgga tatttttatt agtatgataa tatttttaat gctgtggata
    61261 tttattatgt taggtatagt gtgtcttagt tattatataa ctttattatt tagcaaggag
    61321 gcttagtatg tttaaaaaaa taggtataat gagcatttgc atatatataa ttattttata
    61381 ctgcttgaga atgtatcata ttatcaataa tattgaaaca atcttgctaa cggttatatg
    61441 cttaatgtta ttgttttttt taagacgttt atttgataaa gctaagtaaa tagatgttaa
    61501 gtaaaaatgt agaatataaa ggaggggcaa tgagtatgat tgaagttagc catttatcaa
    61561 aaagttttgg tgataaaata gctttaaatg atataagttt cactgttaaa gaaggtcaga
    61621 tttttggatt tttaggacca tctggttctg gaaagaccac aacgattaat attctgactg
    61681 ggcagctcct tgccgataaa ggacaatcta ttattttggg acaaaaatct caaaatttaa
    61741 caagcggtga attaaagaga attggattgg ttagcgatac aagtggattt tatgagaaaa
    61801 tgtctctgta taacaatctt cttttttata gtaaatttta taatattagt aaattacgtg
    61861 ttgataattt gttaaagcga gtaggattat atgatagttg caagatggta gcaggaaaat
    61921 tatccactgg aatgaggcaa cgaatgcttt tagcacgagc tcttatcaac aaacccgctg
    61981 tactctttct ggatgaaccg acctcaggtc tagatcccac aacttctcga acaattcatg
    62041 agttaatttt agaattgaaa acagcaggga caacgatttt tctaacgact catgatatga
    62101 atgaagcaac tcttttatgt gattatgttg ccttattaaa taaagggaaa ttagttgagc
    62161 aaggagctcc ttctgaactc attcaaagat ataataaaga taaaaagatt aaggttacag
    62221 attataatgg gaatcagata acttttgatt ttacatcact agaacaggta tctcaggctg
    62281 atctggaaaa tattttttca attcattcat gtgagcctac tttagaagat atttttatca
    62341 cattaacagg aggaaagcta aatgcttaaa cggtttctgg ctttggtatg gttgcgttgt
    62401 caaattatcc tttccaataa gagtatttta ttgcaagttt tagtgccttt tgctttcaca
    62461 tatttttata aatatcttat ggaaacacag gggaaggtca acgatcaaca ggcattagtt
    62521 cttttgatga tgtgtttacc tttttctttg gctttggctg ttggaagtcc tataactatt
    62581 atcttgtctg aagaaaaaga aaagtacaat ttacaaactc ttctgttgag tggtgttaaa
    62641 ggatccgaat acattttatc aacgatgttt cttccttttt tgctaacttt tgtgattatg
    62701 ggaactactc ctcttatttt aggagttaca attgtacata cttttaatta tattacaatc
    62761 gttcttctaa cctctttatc catcatttta ttctatttat tgataggttt aaccgcgaag
    62821 agccaagtag tagctcaggt tatcagtctt cctgctatga ttttagttgc tttcttaccg
    62881 atgctatctg gtttggataa gacagttgcg aagataacag attatagttt tatgggactg
    62941 tttactaagt ttttcacaaa atgggaggga ttttcatgga atgaaactct aattcctaat
    63001 ctaacactac ttatttggat tgttcttcta ttaactttaa ttacgataac tattaggaaa
    63061 aagaaaattt cttaattgag ttattttaat gattataaac acaagtggga aggaaaaatg
    63121 aactgatctt tttgacagca attctacaga atagtcttat tgctatattt tgatttgagt
    63181 gtacgaaaaa agaaaaataa caatagtgct catactaatt gcagaagttt tgggtgataa
    63241 gataactgat aaattgcaat aaaaaatgca acatttttaa atctcctcta taagtgcttc
    63301 aaaacctgtc ttgtaatcca agtatttttg gggacggtga ttaataagct agcaaagcac
    63361 cattaaggat tttttcggta attgttgcca aatcggttta agaaaatact cacgaagaag
    63421 tccattcgca ttctcattac ttcccctttg ccaagatgaa taggcatccg caaaataaaa
    63481 cagaattccc atttgttcaa ttaaagggta acaagcaaac tctttttctc tgtcctaagt
    63541 gaaagtcttt aactattctt ttggaaagag tcttgtgagg tgttcaatag cagtcaacac
    63601 atacgtgacc atgtttttac ttgacaagtg ctagtagaaa taatagaata gtaaaaaacc
    63661 tttaaagcag tccagagagg cagctaaggt tagacggtga aagggtggag actacccatt
    63721 tttcgtggaa ccttgctgtt ggcaggttcc ttttttcgtg gcttctgttg gccagactct
    63781 ctcactagta aaggtaaaag gagaaaccta tgcgagaaca tcgtccaatc attgctcttg
    63841 attttcctag ttttgaggcg gtcaaggaat ttttagctct tttcccagca gaagaaagcc
    63901 tttatctcaa ggtagggatg gagctttatt acgcagcggg gcctgagatt gtgtcctact
    63961 taaaaggttt gggtcatagt gtctttttgg atctcaaact tcatgacatt cctaatacag
    64021 tcaagtcagc aatgaagatc ctatctcagc ttggtgtgga tatgaccaat gttcatgcgg
    64081 ctggtggtgt agagatgatg aaggctgcgc gtgaaggtct tggaagtcaa gccaaattga
    64141 tcgctgtaac tcagctcaca tcaacgtcag aagctcagat gcaggagttt caaaatatcc
    64201 aaaccagtct gcaagagtct gtgattcact atgccaagaa gacagctgaa gctggcttgg
    64261 atggtgttgt ttgctcggct caggaagtac aagtcatcaa gcaggctacc aatccagatt
    64321 ttatctgtct gacaccaggg attcgtccag ctggtgttgc agttggagat caaaaacgag
    64381 tcatgacacc tgctgatgcc tatcaaatcg gcagtgacta tatcgtagtg ggacgtccca
    64441 ttacccaagc tgaggatcct gttgcagctt atcatgccat caaggatgaa tggacacagg
    64501 actggaatta aagaactaga ttagaaaaat aaaaggagaa taccatgaca cttgctaaag
    64561 atatcgctag ccacctcttg aaaatccaag ccgtttacct caaaccagag gaacccttca
    64621 cttgggcatc tggtatcaag tcaccgattt acactgataa tcgtgtgaca ctagcctatc
    64681 cagaaactcg taccctaatt gaaaatggtt ttgtggaagc tatcaaagaa gcctttcctg
    64741 aagtagaagt gattgcagga actgcaacag cagggattcc acacggagcc attattgctg
    64801 ataagatgga cttgcctttt gcctacatcc gtagcaaacc aaaagaccat ggagctggta
    64861 atcaaatcga aggtcgcgta gctcaaggtc aaaaaatggt agtggttgaa gaccttattt
    64921 caacgggtgg ttcagttctt gaagctgtag cagcagccaa gcgagaagga gcagatgtgc
    64981 ttggagttgt agcgattttc agctaccaat tgccaaaagc agataagaac tttgcagatg
    65041 ctggtgttaa acttgtgacg ctttcaaact atagcgagct tatccatcta gcccaagaag
    65101 aaggttacat cacgccagag ggccttgatc ttctaaaacg ctttaaagac gaccaagaaa
    65161 attggcaaga aggttaggtc agtaagataa agagagacga ggctaccgag cctcttttac
    65221 cattttattt aaaatatgac aatattttaa ataaaataat tgacaacgct ttcttttatt
    65281 gatataatga aaatgtaata tgtatcaaat tgatcaatca actatcttgt ttatcacttc
    65341 ttaagaggac gatgtcatga aaagaagaaa aatatattcc ttttttgcgt agttacaatt
    65401 gcatctagtt tgtttctccc tacagtttta gctagacaga ttggaaatta tgatttaacg
    65461 ttgccgcgtt ggggttcgga tacaactagt gagcttgaga aagaaaacgc ctctgctgga
    65521 attaataata gtgacagcac tggtggcggt aaaaggttaa atacctctat tcgtagcgcg
    65581 cctatagtgg gtcagatatt accccggtat attcattggg gtctggctct aggattgtca
    65641 tgtactataa tggaggtggt gacaattata ttggttctgg tactagatta gctatggcgc
    65701 cacaatttgg aaatcatgta agaattcata cttcaggttc ttggagtcca gattcttatt
    65761 aacttacttg tcagagtaag ccttaaagat ggttgattgt gggtgtagca tgaaaaaaga
    65821 atgctacacc ctatttttat tataaggagg agtaaggatg gaatttttca tttgtaatct
    65881 tgtacgagtc gttcaatcac ctcgatttta tatgtcttta tttttgaccc ttctttgcat
    65941 gagtttagga aatttccttg ctttcaatgg tatttataaa attgaaggtt tatcgatttt
    66001 ttttgccgct tcttctattc gaggattttc accgattagc ctagtagctg cacttatctg
    66061 tacactgccc tattctagtc agataataga ggatgctgag agtcattttc taacagcaca
    66121 attgtgccga atttctaaaa agaggtatct agctattgtg ggtagtactg caattatttc
    66181 ttcttttcta gtcttttttc tcccctattt attattatta ggaattaatc ttttagtgac
    66241 tccttatcag gaaatttata ttggagatta tagtggtgcc ttaaaagaat tatttgattc
    66301 taatcagttg ctctatagtc ttgtgacgac tctctggtat ggagtttggg gcgctgtgtt
    66361 ctctattttt ggactagcta gtgctttgct agtgaagaaa aaaataggag ctattttcat
    66421 cccagttgcc tatatgatgg ttggtggtat tttttgggct attttagggc tatcttactt
    66481 agaacctgtg acaacattag ctttgggata tcagaaagat atcagtcttt ccttagttag
    66541 tgggcatctt gcttttattt tatttgttag ttgtttggtt gtttatggta cattttttct
    66601 acattcagag gactacgtat aatgaaacaa tttgttcaat tttacaaaaa agatttttta
    66661 tcagtattgg tttattttat attactgcta tcctgtgttt tatctagtac agtatattta
    66721 ttgcgctgtc gccaatattc aatccatcca aatgtattag aatggatctt agttttactt
    66781 caagatatga cgactggagt atattgcttt ccgttcacat atatattgtt ctttttttat
    66841 ttgatgaata actattttaa taggttggag tgtcgcattc gtctgaaatc aattaagcac
    66901 tttaccagtt ttagtttcaa attagcagct cttagtacgg ggatttggac ggcgacttta
    66961 tttttattga tttttctaat tgcatttagt aatggtttta gcttctcttt ggagataaag
    67021 gaggttgatt ttttaagaga attttatggt ataagtattg caaacaatgc tagtttcttt
    67081 ataggctttt ttttctctta tatagcatac tatttctttt tatccttact tactattagc
    67141 agtttttctt ggtttaaaaa atcaaacatg agcttagtat ttctgtttac ttttttattt
    67201 gtagaatcct tattctggat ttatcagttg aacaatggga taattggatt attgccaatt
    67261 tttcagtata tggtaaattc caatccgtat gcattgattt attggcttgc attactatct
    67321 atcataattc cattgactgt attttctgtt catagaaact ggaggagagt gtaaaagttg
    67381 gaaatgggaa agttaagtag tcacatatgg aggttgaatc agataatcta taccaagtac
    67441 ttttggggtt atgttctttt ttggatattg atttgtttag gattatggta ttggttagaa
    67501 ggaaatgata gacttgttat agaaatttta aaagggccta atctgagtca aaactctttt
    67561 ttagtcttat ctatatggtt gcttcattgg tttattattc atacattttt tctagcagtt
    67621 gtatatcgta gaagagcatc cgatttcttt ctggaagtga ttcgattttc ttctattaag
    67681 ctctggatta ggtatcagat ttggacctgt tttctttatg gacttatttt aatcatggta
    67741 aaagttctag tgattcaatt tatgttacag ttaccaaact gggatatagg agttttgttt
    67801 atagttgatt ctttgaatgc ttgtgtgcta tccttgcttt gctttatgtt atacgcacta
    67861 ggagcgaatg tacaaatgaa ctttgcttgc gttagtttct ttttactcat gattgtgttt
    67921 ggtggtctat ttgtaggaaa tcgaactaat tatctatttt atattttgaa tcgagggaat
    67981 ggagatatag gacgagattt atttcttcag ttgctatttt tagtctttct ttttaaaagc
    68041 attttctatt tcactcgtca aaagaggagg tttatagaat gaatgaaata attacattaa
    68101 aaaatattga gttgaaatta aaaaaaacat gtgtttttca aaaccttaat tttagttgta
    68161 aacaggggga aattatagga attactggtg cgaatggctc agggaaaagt gtattgttta
    68221 aattaatagc tggtttatat agtccgtctt atggagaagt gttaatcaat ggggaaaata
    68281 ttgttcctga gagaaaaatt ccagctaatt tgggagcttt gattgaagaa cctggtttta
    68341 taaattatta tagtggcttt aagaatttac aatatttggc aagcatacga ggagtagttg
    68401 gtaatcagga aatcaatgat acactgaaaa tagttggtct atatgagcaa aaagaccaga
    68461 aagttaaaac ttattcgcta ggtatgagga aaaagctagg gattgctcaa gcaattatgg
    68521 agaatccctc tattctttta ctagatgaac ctatgaatgc cttggataaa tcaagtgtag
    68581 aaaatatgag aacattgttt agaaagctct ctagtgaaaa agggacaaca attttgattg
    68641 ctagtcatag tgaagaggat attcgtatct tatgtgataa agtatatgca atagaagata
    68701 aagtatgtac actgtgttca gattgaacat cttagataga ctcaggatgt tataggtaaa
    68761 atcttaaaaa gggattggag cttaaaatcc agtccctttt aagagttaat ggccaccttc
    68821 aacaaccagg tcatctgctt tagcagcgtc accgtaggtt gggtgaagtg ttttttcata
    68881 ggccttgtgg aagaagtttt ccttgcctaa tttttcaata tctttattga tgaagtctag
    68941 caattcttgg ttgccttttt gaactgctgc cgcaatggta tcgggatcac cgagggaagt
    69001 aattcctact tcaaatcctt tattttcaag cgcccaagct agaacttccg tattatcagt
    69061 tgaaaaggca tctccacgtc cgtcaagaag agcttggtaa gagtcactgt attggtcgta
    69121 tttttggagt ttgatttctg gatgattctt ttcaaaataa gtctcagcag tcgttccttt
    69181 tgtgacaatt aaggttttac cttcaagttg tttgacgtct gtaatgagac cagtcttagg
    69241 tgatacgaca cccagagaaa ctttcatata tggaagggca aaatcaactt gtttcttacg
    69301 ttcgtcagtt actgtaaagt tagcaagagt aatatctacc ttgtttgaaa tcaagtattc
    69361 cgcacggttg gcagcatcga ctgaaatgta tttaaccttg acaccaaggt cttgagctag
    69421 ttggttccct agttcaatat cgtagccttg gtaagaacca tcattgtcaa cgtagccaaa
    69481 cggtttttta tctccaaaca cggcgattcg cagttcaccg ctttttttgt tttcatcgat
    69541 agtgcgagct ttggcagttg tttttccaga ggatggacca gcgtttccac ctgagctaca
    69601 agcagtgata aagataaggg caaaggcaag tgctaaaaca gttaagagtg gtttgaatag
    69661 tttcataaga atctccttta tagatatgag ccgaattggc taaagtcaaa gacgtttaaa
    69721 aattcctggg ctcgtttggt ttgcggattg gtaaagaagg cttgagctgt tccttcttca
    69781 gcgattttcc cttggtcgag gaagataatc cggtcagcaa tggcttgggc aaactgcatt
    69841 tcgtgggtta ctaaaatcat ggtacggcct tcttgggcca aatcattgat aagttccagc
    69901 acctcacgca ccatttctgg atccagcgaa gcagtcacct cgtcaaaaag gatgatttct
    69961 ggatgcatta ggagggcacg gacaattgca acacgttgct tctgtccacc agataattga
    70021 cgggcaaagc tatgttgttt atccagcaaa ccgacacgat ctagtaattg caaagcttct
    70081 tccgttactt ctttcttgtc ccttccttga gttttgatag ggcctaggat gaggttttgt
    70141 aagacatcta gatggggaaa gagttcataa ctttgaaaga ccatgccaat cttttggcga
    70201 actaggtgaa aatctttttt attttcaacg atagactgac catccagaaa aatatctcca
    70261 ccttgaatac tttctaagcc gttgaggcaa cgaaggaggg tacttttccc acaaccagat
    70321 ggccctagga tgacaacaac ttcccttttt tgatttctag agaaagtcct tggaggatgg
    70381 gattgtctcc gaaggatttt tttagttctt tgatttctaa gatagtttca gacatttagt
    70441 ttctccaatg tttttctaag tgagtggata gtttggaaat agggtagcaa actgcgaaat
    70501 ataagactag aatggttcca taaatccaaa atgaagcagt tgggatggtc aggcgattgc
    70561 tatcgatgat ttgttgtcca actttggtca cttccacaac cccaatcaaa acaactaatg
    70621 aagtggtttt aatcatccta gtgacaagat tgatagcctg cggtagcagt cttcttaaga
    70681 cttgtgggat gatgatgtgg tagtaaagtt gaacattagt caagccgagt gactgtccac
    70741 tttcaaactg atgtttaggg agagaagtga tagctccacg taccaagtct cccatttcag
    70801 ctgttcccca gagggtaaaa acgataatag ctgaagtctc acctgagata ttgatattaa
    70861 agtttcgagc caagccaaag taaacgatga agagtagcac cagctggggc atgatacgga
    70921 taaattccag atacaatcgt gttaaaaatc gtatgattct agaatgggag gtcatgatga
    70981 ttcccatgac tgttccgaac atcatggata agaggacaga caggatggat atcccaatcg
    71041 taacgcccaa tccctgtaag attctcagga gattatttcc ttgaaagagt acttggattc
    71101 ccgaatcctg catggggagc ctcctttcta tccagctaaa gaccagtgag atgggtagca
    71161 gcatgatcag ataagcaact accaacatag ctagcgcaat gtctgtctca tagtagagac
    71221 caatcaaatc cttggcgacg tacatgaggt cggccaaagc cactgctgag aaaacagagg
    71281 tttccttgat aaggaaaatg acattggcac taaaggaggg gagtgccacc gctgttgctt
    71341 gcggaagaac cacatagtaa aagacctgta gaggtgtcag accaatagcg aggccaatct
    71401 cctgctgggt ttgactgatg gcttccagcc cacttcggaa agattctgcc atataggagc
    71461 ctcctaaaaa gacaagccca agcgttgcac agacttctga agataggaca atcccgattc
    71521 ggggaagacc gaagtagaga aagaagagtt gaatcaaaag gggcgtatta cgtgacaatt
    71581 caatgtaggc tgtcgctact tgcgccaaaa caaggattcg ataatgtcgg atgatactaa
    71641 cgattaaacc gagcagaaag gatcccaaaa ttccccaaac tgcaatatgc aaggtcagaa
    71701 agaatgcctt ttgatatagt ggtagatatt gttcaacaat ggatcaatcc aaaaatagaa
    71761 cctcccatct agaaataata cagttattgt agcacttaaa atcttctttg gataatatct
    71821 attttttatt gccgttataa ggatttttat catagacata aaatttctga aatttccaaa
    71881 caaaatattt taaaagtttt gaaaaagagt taagatattt ttgtaataca caaagtaaac
    71941 gcttacttat taaggaggac attttatgtc atacaaaaca agcaatgcag aaggtcatgt
    72001 agatttcatc aatacctatg atttggagcc aatggcgcaa caagttattc ctaaagcagc
    72061 atttggctat atcgctagtg gggcgggaga tactttcact tctttccagt gattttagcg
    72121 tcaggttctt tttagttttt aaagattatc cgtgaatttc ttgcttattt atgataaaat
    72181 gggagtatcg caaaaaatga ctcatcgtat tcaattttga gtaaaactag gaggatccca
    72241 tgtctacaga acatatggaa gaactaaatg accagcagat cgttcgccgt gaaaaaatgg
    72301 ctgcgctccg cgaacaagga atcgatcctt tcggaaaacg ttttgaacgt actgcaaatt
    72361 cacaagaatt aaaagataaa tatgccaacc tcgataaaga acaattacac gataaaaacg
    72421 aaacagctac tatcgcagga cgcttgataa ccaaacgtgg taaaggaaaa gttggttttg
    72481 cccaccttca agaccgcgaa ggccagattc agatctacgt tcgtaaggat gctgtcggtg
    72541 aagaaaacta cgaaatcttc aaaaaagcag accttggtga cttccttggt gtcgaaggtg
    72601 aagtgatgcg tacggatatg ggagaactct ctatcaaggc aacccacatc acacacttgt
    72661 ctaaggctct tcgtcctctt cctgagaaat tccatggttt gacagacgtt gaaacaattt
    72721 accgtaaacg ttaccttgac ttgatttcta atcgtgaaag ctttgaacgc tttgtcactc
    72781 gttcaaaaat catctctgaa atccgtcgtt accttgacca aaaaggattc cttgaagtgg
    72841 aaacacctgt tcttcataat gaagccggtg gtgctgctgc ccgtccattt atcacccacc
    72901 acaatgccca aaacattgac atggtgcttc gtatcgcgac tgagcttcac ttaaaacgcc
    72961 ttattgtggg tggtatggaa cgtgtctatg aaattggccg tatcttccgt aacgaaggaa
    73021 tggacgctac tcataaccct gagttcactt ctatcgaagt ttaccaagct tatgcagact
    73081 tccaagatat catggacttg acggaaggca ttatccaaca tgctgctaaa tcagttaagg
    73141 gcgatggccc agtcaactac caaggtactg aaatcaagat taacgaacca tttaagcgtg
    73201 ttcatatggt ggatgctatc agagaaatta ctggtgtcga tttctggcaa gacatgactt
    73261 tggaagaagc taaagctatc gctgctgaga agaaagttcc agttgagaaa cactacactg
    73321 aggttggtca catcatcaat gccttctttg aagagtttgt tgaagaaact ttaatccaac
    73381 caacctttgt ctatggacat ccagtagctg tatctccact cgctaagaaa aatcctgaag
    73441 accaacgctt tactgaccgt ttcgagctct ttatcatgac taaggagtac ggtaatgcct
    73501 ttactgagtt gaacgaccca atcgaccaac ttagccgttt tgaagcccaa gctaaagcca
    73561 aagaacttgg tgatgatgaa gcgacaggaa tcgactatga ctacatcgag gctcttgaat
    73621 acggtatgcc accaacaggt ggtttgggaa tcggtatcga ccgtctctgc atgctcctca
    73681 ctgatacaac aactatccgt gatgtattac tcttcccaac aatgaaataa attcttatcc
    73741 tctgggtctt atcagaggat tttttgattc aaaaagagac tgaatttaag gagaaaatga
    73801 agtgtagtat attgaaatag tacactttga tttctaagac attgttagaa attggtttaa
    73861 attccctaag caatttgtgc atgttttatt tcattttacg atagtacgct gaaacttttc
    73921 aaaaagtact agaaattgac ttggattccc caattgattt gttcagattc actataaata
    73981 aaaaattaat aagtgggata ggaagttagc gtcaactagg atagtatctt gcttaaacag
    74041 tatatatggg actgatataa gtccataggt cctattagag gatgttctgg tgtcttattc
    74101 acttgctttt ttatagtatt agtagataga atcagcaaat aaaaaagtag ttcaaatgcc
    74161 tggcagcaaa aaatctcaac gattcactta atatagacta aagtttagag tttaaggaaa
    74221 gtagggctag tatattgtat gaaaaaataa gatggttttt aagcaaaact cttgaaaatg
    74281 attgaaaaaa gagttattat agttgtgtag taaaaagcaa acgcttactt aatcaaggag
    74341 ggcattttat gtcatacaaa acaagcaatg cagaaggtca tgtagacttc atcaatacct
    74401 atgatttgga gccaatggcg caacaagtta ttcctaaagc agcatttggc tatatcgcta
    74461 gtggggcgga agatactttc actttaagag agaatatccg cgcctttaac cacaaactca
    74521 tcgttcctca tacactttgc gatgttgaaa atccaagtac agagattgaa tttgcaggtg
    74581 aaaaactatc ttcaccaatc attatggcgc ctgttgcggc tcataaattg gcaaatgaac
    74641 agggggaagt ggcgactgcg cgtggtgtgc atgagtttgg ttctctttat acaaccagtt
    74701 cttactctac tgttgacctt ccagagattt ctgaagccct tcaagggaca cctcattggt
    74761 ttcaatttta ctttagtaag gatgacggta tcaaccgcca catcatggac cgtgtgaagg
    74821 ctgaaggtta taaagcgatt gtcttgacgg cagatgctac tgtagggggg aatcgtgaag
    74881 tggataagcg taatggtttt gtcttcccag ttggcatgcc gattgttgaa gaatacctgc
    74941 cagaaggtgc tggtaaatca atggactttg tttacaaatc agctaaacaa cgcttgtctc
    75001 cacgcgatgt agaatttatc gctgaatact ctggtcttcc tgtgtatgtc aagggaccac
    75061 aatgccgtga ggacgttgaa cgttcgcttg ctgcaggagc ttctggtatc tgggtaacca
    75121 accacggtgg tcgtcaaatc gacggtggac cagctgcctt tgactcgctt caagaagtgg
    75181 cagaagcagt tgatagacgt gtgccgattg tctttgactc tggtgttcgt cgtggtcaac
    75241 acgtctttaa agccttggca tcaggagcag acttggttgc tattggacgc cctgtcatct
    75301 atggtttggc tctcggtggt agtgtcggtg tgcgtcaagt ctttgaacac ttgaatgcgg
    75361 aattgaagac agttatgcaa ttgtctggag ctcagaccat tgaagatgtc aaacacttca
    75421 agctccgtca caatccatac aacccaacct tcccagttga ccctcgtgac ttaaaattgt
    75481 attgataaaa cagattgcct ccactgaatg tggaggtttt tcttcgtgta ttggaagaat
    75541 aaattgctat cgaaaactat agtaaaatga aataa
//
LOCUS       DAVMMR010000003        69075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMMR010000003 DAVMMR010000000
VERSION     DAVMMR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.003"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            1..382
                     /locus_tag="VYS74_000155"
                     /pseudo
     CDS             1..382
                     /locus_tag="VYS74_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            385..1398
                     /locus_tag="VYS74_000156"
                     /pseudo
     CDS             385..1398
                     /locus_tag="VYS74_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            1373..1951
                     /locus_tag="VYS74_000157"
     CDS             1373..1951
                     /locus_tag="VYS74_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV3788254.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(2008..2292)
                     /locus_tag="VYS74_000158"
     CDS             complement(2008..2292)
                     /locus_tag="VYS74_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV3788255.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2346..3281)
                     /locus_tag="VYS74_000159"
     CDS             complement(2346..3281)
                     /locus_tag="VYS74_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV3788256.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KKHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3385..3828
                     /locus_tag="VYS74_000160"
     CDS             3385..3828
                     /locus_tag="VYS74_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV3788257.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            3944..4210
                     /locus_tag="VYS74_000161"
     CDS             3944..4210
                     /locus_tag="VYS74_000161"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV3788258.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            4207..4581
                     /gene="crcB"
                     /locus_tag="VYS74_000162"
     CDS             4207..4581
                     /gene="crcB"
                     /locus_tag="VYS74_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3788259.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            4575..4904
                     /gene="crcB"
                     /locus_tag="VYS74_000163"
     CDS             4575..4904
                     /gene="crcB"
                     /locus_tag="VYS74_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3788260.1"
                     /translation="MVIVYLAIACGFGALVRYLFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            5023..5370
                     /gene="rplS"
                     /locus_tag="VYS74_000164"
     CDS             5023..5370
                     /gene="rplS"
                     /locus_tag="VYS74_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV3788261.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            5412..5483
                     /locus_tag="VYS74_000165"
     tRNA            5412..5483
                     /locus_tag="VYS74_000165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5445..5447,aa:Arg,seq:cct)
     gene            complement(5538..6344)
                     /gene="yidA"
                     /locus_tag="VYS74_000166"
     CDS             complement(5538..6344)
                     /gene="yidA"
                     /locus_tag="VYS74_000166"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV3788262.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(6357..7661)
                     /locus_tag="VYS74_000167"
     CDS             complement(6357..7661)
                     /locus_tag="VYS74_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV3788263.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            7733..8110
                     /locus_tag="VYS74_000168"
     CDS             7733..8110
                     /locus_tag="VYS74_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV3788264.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8201..8533
                     /locus_tag="VYS74_000169"
     CDS             8201..8533
                     /locus_tag="VYS74_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV3788265.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            8545..10116
                     /gene="ffh"
                     /locus_tag="VYS74_000170"
     CDS             8545..10116
                     /gene="ffh"
                     /locus_tag="VYS74_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV3788266.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(10311..11594)
                     /locus_tag="VYS74_000171"
     CDS             complement(10311..11594)
                     /locus_tag="VYS74_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV3788267.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFIPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(11897..12610)
                     /gene="rsmG"
                     /locus_tag="VYS74_000172"
     CDS             complement(11897..12610)
                     /gene="rsmG"
                     /locus_tag="VYS74_000172"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV3788268.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12704..13264
                     /locus_tag="VYS74_000173"
     CDS             12704..13264
                     /locus_tag="VYS74_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV3788269.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            13266..14165
                     /gene="htpX"
                     /locus_tag="VYS74_000174"
     CDS             13266..14165
                     /gene="htpX"
                     /locus_tag="VYS74_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV3788270.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(14314..15873)
                     /locus_tag="VYS74_000175"
     CDS             complement(14314..15873)
                     /locus_tag="VYS74_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3788271.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(16112..16429)
                     /locus_tag="VYS74_000176"
     CDS             complement(16112..16429)
                     /locus_tag="VYS74_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV3788272.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKIHYEYVKHVLSLYGPV
                     FGF"
     gene            16599..17141
                     /locus_tag="VYS74_000177"
     CDS             16599..17141
                     /locus_tag="VYS74_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV3788273.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            17141..17770
                     /gene="nth"
                     /locus_tag="VYS74_000178"
     CDS             17141..17770
                     /gene="nth"
                     /locus_tag="VYS74_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV3788274.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            17981..18502
                     /gene="pyrR"
                     /locus_tag="VYS74_000179"
     CDS             17981..18502
                     /gene="pyrR"
                     /locus_tag="VYS74_000179"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV3788275.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            18521..19444
                     /locus_tag="VYS74_000180"
     CDS             18521..19444
                     /locus_tag="VYS74_000180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV3788276.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            19494..20573
                     /gene="carA"
                     /locus_tag="VYS74_000181"
     CDS             19494..20573
                     /gene="carA"
                     /locus_tag="VYS74_000181"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV3788277.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            20885..24061
                     /gene="carB"
                     /locus_tag="VYS74_000182"
     CDS             20885..24061
                     /gene="carB"
                     /locus_tag="VYS74_000182"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV3788278.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(24336..25145)
                     /locus_tag="VYS74_000183"
     CDS             complement(24336..25145)
                     /locus_tag="VYS74_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3788279.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(25147..25950)
                     /locus_tag="VYS74_000184"
     CDS             complement(25147..25950)
                     /locus_tag="VYS74_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3788280.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(25960..27447)
                     /gene="tacF"
                     /locus_tag="VYS74_000185"
     CDS             complement(25960..27447)
                     /gene="tacF"
                     /locus_tag="VYS74_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV3788281.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            27783..28490
                     /locus_tag="VYS74_000186"
     CDS             27783..28490
                     /locus_tag="VYS74_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV3788282.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            28495..29517
                     /locus_tag="VYS74_000187"
     CDS             28495..29517
                     /locus_tag="VYS74_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3788283.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            29534..30403
                     /gene="licA"
                     /locus_tag="VYS74_000188"
     CDS             29534..30403
                     /gene="licA"
                     /locus_tag="VYS74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV3788284.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIGSAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            30393..31271
                     /locus_tag="VYS74_000189"
     CDS             30393..31271
                     /locus_tag="VYS74_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV3788285.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            31283..31972
                     /locus_tag="VYS74_000190"
     CDS             31283..31972
                     /locus_tag="VYS74_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV3788286.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            32068..32916
                     /gene="dprA"
                     /locus_tag="VYS74_000191"
     CDS             32068..32916
                     /gene="dprA"
                     /locus_tag="VYS74_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV3788287.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            33805..34848
                     /locus_tag="VYS74_000192"
     CDS             33805..34848
                     /locus_tag="VYS74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV3788288.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            35015..37102
                     /gene="topA"
                     /locus_tag="VYS74_000193"
     CDS             35015..37102
                     /gene="topA"
                     /locus_tag="VYS74_000193"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV3788289.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            37208..37567
                     /locus_tag="VYS74_000194"
     CDS             37208..37567
                     /locus_tag="VYS74_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV3788290.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            37660..38292
                     /locus_tag="VYS74_000195"
     CDS             37660..38292
                     /locus_tag="VYS74_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV3788291.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVVVTQLHGTKVVF"
     gene            38336..38992
                     /locus_tag="VYS74_000196"
     CDS             38336..38992
                     /locus_tag="VYS74_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV3788292.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            39011..39643
                     /locus_tag="VYS74_000197"
     CDS             39011..39643
                     /locus_tag="VYS74_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV3788293.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            39671..40105
                     /locus_tag="VYS74_000198"
     CDS             39671..40105
                     /locus_tag="VYS74_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV3788294.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            40117..41154
                     /gene="leuB"
                     /locus_tag="VYS74_000199"
     CDS             40117..41154
                     /gene="leuB"
                     /locus_tag="VYS74_000199"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV3788295.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            41151..41420
                     /locus_tag="VYS74_000200"
     CDS             41151..41420
                     /locus_tag="VYS74_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV3788296.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            41424..41783
                     /gene="leuD"
                     /locus_tag="VYS74_000201"
     CDS             41424..41783
                     /gene="leuD"
                     /locus_tag="VYS74_000201"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV3788297.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            41945..42568
                     /locus_tag="VYS74_000202"
                     /pseudo
     CDS             41945..42568
                     /locus_tag="VYS74_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            42734..44647
                     /locus_tag="VYS74_000203"
     CDS             42734..44647
                     /locus_tag="VYS74_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV3788298.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSGVSGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQ
                     TGQQDTGDNDADY"
     gene            44634..45950
                     /locus_tag="VYS74_000204"
     CDS             44634..45950
                     /locus_tag="VYS74_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV3788299.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            46125..47111
                     /locus_tag="VYS74_000205"
     CDS             46125..47111
                     /locus_tag="VYS74_000205"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV3788300.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            47175..47246
                     /locus_tag="VYS74_000206"
     tRNA            47175..47246
                     /locus_tag="VYS74_000206"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47208..47210,aa:Arg,seq:ccg)
     gene            47429..48127
                     /locus_tag="VYS74_000207"
     CDS             47429..48127
                     /locus_tag="VYS74_000207"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV3788301.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            48118..51657
                     /gene="smc"
                     /locus_tag="VYS74_000208"
     CDS             48118..51657
                     /gene="smc"
                     /locus_tag="VYS74_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV3788302.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQDEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            51654..52448
                     /locus_tag="VYS74_000209"
     CDS             51654..52448
                     /locus_tag="VYS74_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3788303.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            52448..53266
                     /locus_tag="VYS74_000210"
     CDS             52448..53266
                     /locus_tag="VYS74_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3788304.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            53270..54559
                     /gene="ftsY"
                     /locus_tag="VYS74_000211"
     CDS             53270..54559
                     /gene="ftsY"
                     /locus_tag="VYS74_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV3788305.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(54603..56090)
                     /locus_tag="VYS74_000212"
     CDS             complement(54603..56090)
                     /locus_tag="VYS74_000212"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV3788306.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(56239..56979)
                     /locus_tag="VYS74_000213"
     CDS             complement(56239..56979)
                     /locus_tag="VYS74_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3788307.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(56979..59144)
                     /locus_tag="VYS74_000214"
     CDS             complement(56979..59144)
                     /locus_tag="VYS74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3788308.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            59314..60267
                     /locus_tag="VYS74_000215"
     CDS             59314..60267
                     /locus_tag="VYS74_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3788309.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            60344..62332
                     /gene="uvrB"
                     /locus_tag="VYS74_000216"
     CDS             60344..62332
                     /gene="uvrB"
                     /locus_tag="VYS74_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV3788310.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            62343..62906
                     /locus_tag="VYS74_000217"
     CDS             62343..62906
                     /locus_tag="VYS74_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788311.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            62872..63360
                     /locus_tag="VYS74_000218"
     CDS             62872..63360
                     /locus_tag="VYS74_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788312.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            63434..63901
                     /locus_tag="VYS74_000219"
     CDS             63434..63901
                     /locus_tag="VYS74_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3788313.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(63898..64413)
                     /locus_tag="VYS74_000220"
     CDS             complement(63898..64413)
                     /locus_tag="VYS74_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV3788314.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(64425..64952)
                     /locus_tag="VYS74_000221"
     CDS             complement(64425..64952)
                     /locus_tag="VYS74_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3788315.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(65234..65797)
                     /locus_tag="VYS74_000222"
     CDS             complement(65234..65797)
                     /locus_tag="VYS74_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3788316.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(65781..66332)
                     /gene="coaC"
                     /locus_tag="VYS74_000223"
     CDS             complement(65781..66332)
                     /gene="coaC"
                     /locus_tag="VYS74_000223"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV3788317.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(66344..67033)
                     /gene="coaB"
                     /locus_tag="VYS74_000224"
     CDS             complement(66344..67033)
                     /gene="coaB"
                     /locus_tag="VYS74_000224"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV3788318.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            67263..68933
                     /locus_tag="VYS74_000225"
     CDS             67263..68933
                     /locus_tag="VYS74_000225"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV3788319.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        20927 a        12519 c        15209 g        20420 t
ORIGIN      
        1 aatgtaaaaa aatatgagga gttcggactc gactctctcc ttcaagaaac acgtggtggt
       61 cgtaaccatg catatatgac agttgaggaa gagaaagcct ttcttgcccg tcatttgaag
      121 gctacagagg caggagaatt tgttacaatt gatgccttat ttcaggctta taaaaaggag
      181 ttaggtcgtt cctacacacg tgatgccttc tatcaactgt tgaagcgcca tggttggcga
      241 aatattacac cacgtccaga acatcctagg aaagcagacg ctcaaaccat tgtcgcgtct
      301 aaaaataaaa tctcaattca agaagacaag aaagcgcttt aaaaccagta gaccttttca
      361 taaggttcgc ttgatgtacc aaatagtacg cccatgcaaa agaatatcta ttttgttgtc
      421 ttggacctgc atacgacgga tagagataaa attatccagc tattcaagga ttggacggat
      481 tatagtgcca agttggtcga gggggaattg gttaaaaaag atggacagaa tgctctcttt
      541 cctccgagcg atactggtga gacagttggg ctcaatcctc atcgtttgac gctgactttt
      601 ggtgtatctg caagtttctt gaaaaggatg aacttggaaa acaagcgccc tcgactgttc
      661 agggatttac ctctatttcc taaggagcaa ttacgtgaaa aatatactgg tggagatatt
      721 gtgattcatg cttgtgcaga tgatgagcaa attgcctttc acgctattcg caatctcatc
      781 cgtaagggga gaaatgcggt gccccttcgt tggagtcagt ctggatttgc agctatcggg
      841 gatcgaatgg agactccgtg gaacctcttt ggttttaagg atggaacagc aaatccaacc
      901 aaggagcaag actttgaccg cgttatctgg gctgatagta aggactggat ggaaaatggt
      961 tcttatatgg cagttcgtcg gattcagatg tttttagaga cctgggaccg cactagtctc
     1021 gaagaacaag aaaatacctt tgctcgttac aaggaaagcg gtgccccctt tggtaagaaa
     1081 aatgagtttg atgaagtgga tttgagtctc ttacctgatg attcgcatgt ttgtttggcc
     1141 aaagaagtgg ataagccact tttacgtcgt tcttattctt actcagatgg cattgatgaa
     1201 aagactggtc agtttgatac gggattgctc ttcatttctt tccagaagga tcctgataac
     1261 tttgttaagg ttcaaactaa cctaggtgct actgataaga tgaatgaata catcactcac
     1321 ataggtagtg gactttttac ttgctttggt ggtgtggaga aaggaggcta cattggtcag
     1381 aaattattgg aaggctaaaa gctggttact agttttagct ttgtcatttt taattctttc
     1441 cccagcaggt gcccaagaaa gcttgagttt tttctttgtg aaaatcacag atgcatccaa
     1501 aacggttaaa aatggtggtc agactgagac ccagaaactc gttacgaaga tggcatcaga
     1561 cttcgaaaga gtagaaaata aggattctga ggttggtaag attgttaagg aaaagttagc
     1621 tctatctgga gatattactg aagccaaact caccgaaatt tcctcagctc tattagcttt
     1681 tgaaaaagaa caaaatcctg ttgacctaga tgcggaaaag gaaaaattgg taaaccgtct
     1741 aagtcctcgt tttgaaacct tagaacaggc cattgcctcc aaagatttgg aaaaagtccg
     1801 agaagctttt aagaaaatga attccacttg gaccatcaat gaaagtgtgg ttcgtgacaa
     1861 tagtacagct cattatggac gtgttgaaac agctatttcc ttcctgccta gtagcatgga
     1921 aaccgaacct acagatgagt ctggaacatg aaaacagcag ttgatttcaa gaaacaaaaa
     1981 agaacagccg aaactgttct cttatcatta tttgagaccg tattttttgt tgaaacgatc
     2041 cacgcgtcca tctgcttgag tgaacttttg acgtccagtg tagaatgggt gtgagtctga
     2101 tgaaatttcc acacggatca atgggtaagt ttcgccttcg aactcaactg tttcgttaga
     2161 gcgttttgtt gaaccactaa ggaattggta accagtagtt gtgtccatga agacaactgg
     2221 gcgatattct ggatggatat cttttttcat tttgcaatat ttcctttctg ccatggtctc
     2281 tttgcgagcc atagattgtt accatactag tctatcagat tctgaaaagt ttggcaagta
     2341 ttttatcagt ttttaagcaa gttttttaac ttttggtaga tgatttcgtt ttcctctagg
     2401 ctataggaat tagcaccact tgctagaggg tggcctccac catcatgttt cttggcaatt
     2461 tcattgatag gatggatttt actgcgtaag cgaactcggt agtggccatc agcctgttcg
     2521 acaaaaattc cccagagact cactctgtca atgcgtccag gtgcaccgac aatggctgca
     2581 gtttcagcat cggttacatt gtattgtttc aagacttctt gactcaggat aacgcgagca
     2641 gcaccatttt catccacttc cagatggtcg tagatgtagc cttgcagttt agcaattttg
     2701 tagctcatag tgtccatttt gcgagtgaga gccgcaaagt caaagttatg ttctctcaaa
     2761 taagcagcca ggcgaagagt ccgtgcagtg gtagaagggt agaggaagcg acctgtatca
     2821 ccgacaattc ctgcaaagag caactcagca tcgcgatctg ccaaggctag ttgggttgtt
     2881 tgggcaaata gggtaatcat ctcgctagcg ctacttgaac tagtatcgac ccaagacagg
     2941 tcaccgtata catcatcatt tggatggtgg tcaatcttaa tgagaaaatc accttgacta
     3001 tagcgcttat catcgatacg agcagtatta gctgtatcac agacgatgac aagtgcgcct
     3061 tggtaggctc tatcttcaac aagatccatc tcagccatcc aagtaagagt tggttcatca
     3121 aaaccgacgg ctttgatggt tttttctggg aaatgatgtt ccagcaaggc tttcaatccc
     3181 acctgacttc ccaaggcatc agggtctggt ttcatatgac gatgaatgat aatggtgtcg
     3241 tattctttaa ttttttctaa aatttgttgg caaatctcca taaataacct caattctttc
     3301 tcatttcatt gtagcacaag aatttttatt tttaaaatga aaaagatgtg atataatgga
     3361 gaaagataaa ttgaggagga cgatatggca ttagcaaaaa ttgtatttgc cagtatgacc
     3421 ggtaataccg aagaaattgc agatattgta gcagacaaat tacgtgactt gggcttggat
     3481 gttgatgttg atgaatgtac aactgttgac gcttcagact tcttggaagc agacatcgct
     3541 atcgttgcga cctatactta tggtgatggt gaattgccag atgagatgat ggacttctac
     3601 gaagatctag cagatctcaa cttgaatggc aaaatctacg gagtggtcgg ctcaggagat
     3661 accttctacg atgaattctg taaggctgtt gatgactttg accgtgtctt tgtttcaaca
     3721 ggagcagaaa aaggttctga gtgtgtcaaa gttgatcttt ctgccgagga agaagatatt
     3781 gaacgcttgg aacaattcgc agaagaattg gttgctaaag taggataagc ataaaagtgc
     3841 gctgatgtaa tcaatcagtg catttttctt atcgagagtt gggattttac tagcctaatt
     3901 gatggctttt tgatacaata attcaaataa aggaggagac tgtatggatt tagatattat
     3961 tcgtcaagaa atcgatcaaa tcgacgacca aattgtcaaa ctgttagaag aacgaatgca
     4021 tttagttgag ggggtggttg cttataagaa agcttcaggt aaaccgattt tagatactaa
     4081 gagagaagaa gttatttttg aaaaggttgg gagtcgtgta gaggacaagc gctatcagga
     4141 gactattgta gcgacttttt cggacatact caaacgttcg cgtgattatc aggatcaaaa
     4201 tatcaaatga aaaaagaaca attttatccg ctaggaattt ttctagctgc tatgttgggc
     4261 ggacttgtcc gatatctagt ttccacctgg ttaccagcca gtccagactt tccttggggc
     4321 actctctttg tcaactatct gggaattttc tgcttgattt ttcttgtcaa gggctatctg
     4381 gtctataagg ggactagtaa gggcttgatt ttagcactgg ggacgggttt ttgcggaggt
     4441 ttaacaactt tttccagcct aatgcttgat actgtgaagc tgcttgatac agggcgttat
     4501 tttagtttag tcctgtattt gcttttgagc atcggtggag gcctactctt agcttatttt
     4561 ctagggagga agaaatggta atcgtctatc ttgcaattgc ttgtggtttc ggagctttag
     4621 tgcgctattt attttctcgc tataatcaag cttctaaatt gccactcgga acgctcatag
     4681 ctaatctttt aggttgtttt ttaattggag tattttacaa tcatgtagag tctaaggaag
     4741 tatatgctat tctagcaaca ggattttgtg gcggtttaac aactttttcg accttgaatg
     4801 acgaacttca aagactgcta agtgataaga aggtctttta ttcttacctg actttgactt
     4861 acataggtgg tttggttgcg atttttttag gaattttgct ataaatttct ttactttttt
     4921 ttagaaattt ggtaaactgt atcttgtgtg taatggacgc acaaaaatac agtttatccg
     4981 ctgaggaaga ttcctcaaga ttgacaaaga taggagaata aaatgaatcc attaatccaa
     5041 agcttgactg aaggtcaact tcgtacagat atcccatcat tccgtcctgg tgacactgtt
     5101 cgtgtacacg cgaaagttgt cgaaggtaac cgtgaacgta tccagatttt tgaaggtgtt
     5161 gttatcgcac gtaaaggtgc tggaatctca gaaaactaca cagttcgtaa aatctctaac
     5221 ggtgtaggtg ttgagcgtat cttcccaatc cacactccac gtgttgaaaa aatcgaagtt
     5281 gttcgttacg gtaaagtacg tcgtgcgaaa ttgtactact tgcgtgctct tcaaggtaaa
     5341 gcagctcgta tcaaagaaat ccgtcgttaa ttcgactaaa aaactctgct tttcagcaga
     5401 gtttttatct agctccctta gttcaatgga tataacaact ccctcctaag gagtagttgc
     5461 aggttcgatt cctgcagggg gcagtaatta gacatcaaaa accgctcagc tgagcggttt
     5521 tagtctattc tttcctttta cagtacccat gttcggatgg cgtgggcaac gccggattcg
     5581 tcatttgttt tagtgatgta tttggcgatt tttttgattt ctggatttcc attttccatg
     5641 acaacggggt ttccaacgac ttccagcatg gcacggtcat tttcttcgtc accgattgcc
     5701 atggtttcat ctttggtcaa tccgagtttt tcagccaagt gagtaatggc tgaacccttg
     5761 tctacattct ttgtaaggag ttcgaggtag aaaggagcag atttgttgat ggagtagcgc
     5821 tcgtaaaatt ctgctggaat tttttcaatc gcagcatcga gaatttctgg ttcatcgata
     5881 aacatacatt taacaatttc tttgccagcc atttcttcag gggtacggta gaagataggc
     5941 atgctgacga gggttgattc gtgtacagtg tatgttccga tattgcgatt tgcagtatag
     6001 ataccgtcct tggtaatggc atgcatgtgg acaccgagct tgcgactgag gaattccata
     6061 tccagataat cctcataggt caaggattcg ctgataatct catggccagt agcagtttct
     6121 tggacaaggg caccattgaa ggtcaccaca tagtcacctt ggtctctcaa ctgcaagtcg
     6181 tccagaagtt tggcaacacc tgcgatgggg cggccagttg caatcacgac tttgacacca
     6241 gcttctttgg catcttggat ggcagaaaag acttcaggag taatctcctt ttgactgttg
     6301 actagggttc cgtcaatatc gacagcgatt agtttaatac tcataaaatt cctcttctag
     6361 tttttatttg gggtaaaatg atcgttctca atcaagtgta aaaattgctg ggtaatgctt
     6421 gcaaagatgc tgttttggtc caacatttct tttggaaaat aaaagcgatt atctccgtgg
     6481 cgactgccag caagggattg gacgatagga gacaggctag agagttcggc cagttctcca
     6541 tttttttgta aaatctcaat ctgtgtccgt ggattttgag attcgggacg atagatatca
     6601 taagggaggt caaagttctt atgaatggca gtatagtagt cgggatcaaa gccgatgtcc
     6661 tcaaccaatt ttctcatgct agtaagttga tcttggtcct cttgtgaaaa ggtaatggat
     6721 ttaaagacct tgcggttgac aaagcgttga gataaatctg caagaatttt gtcaggacta
     6781 gtcatccaaa gctggaagta ggtattcatc acgccatcat ccagagccag atagtcagtc
     6841 aaggtcacat ttttttcgaa gaaaggcagg agatgtgggg aagttcgtgc aaagaaattc
     6901 ttgtcctcag ggtagagttc cttggcgcgc ttgagaagat tctgtaggag aacttccatg
     6961 gcgcgtgttg ctgggtggaa ataaacctgc atgtacatct ggtagcgact gaggacgtag
     7021 tcttcgatgg cgtgcatgcc attgcgctga aaggcgatac cattttcgac aggacgaatg
     7081 actcggagga tgcgagtcag gtcaaattcc ccataggatg ctcctgtaaa ataggagtcg
     7141 cgcaagagat agtccatgcg atctgcgtca atctgactag aaatgagctg cacgacctgc
     7201 ttattaggat aggtatggtc tatgacactg gcaacttttt ctggaaaatc gggcgctact
     7261 tgtagcaaga cttggtgaat ctctgtctca ggattttgaa taatctcctg agtaatggct
     7321 tcatggtctg tatcaaagag atgttcaaaa gtatgggagt aggcgccatg cccaaggtca
     7381 tgtaggagag cagcggtcat ggtcaagaga gactcggcag gattccattc ctcaggatat
     7441 ttttcttcaa aaatttctgt gatacgtcgt gcaatctcat agactcctag acagtgagag
     7501 aagcgactgt gttctccacc gtggaaggta taactggaag ttcccagttg tttgatccgg
     7561 cgcaaacgct gaaattcttt tgtgttaatc aagtcataga tgatttgatt attgacatgg
     7621 atgtagttgt gaactggatc acggaatact ttttcgttca tatgaccatt atagcaaaat
     7681 cctgctcttt ttggtaaaat agtaggacaa gagacaagaa agaggacctg atgtgaagat
     7741 tcggatgcga aatacaattc agtttgatga gcagttggaa gtgattgacc agctttatga
     7801 tgtggaagtg catgaaaaag gagattatag ctacctgctt ttctataatg aggaaaagga
     7861 aaaagtagtg attaaatttc atggtcaaga acttgtgatg agccgatttt ctaatcccaa
     7921 gaccattatg cgctttctaa aggatagcga tagtttagcc tatattccta cacctatggg
     7981 catgcaggag tttatcatcc aaacgagcca ttatcaagtt gataggcaaa agattgaact
     8041 agattatcaa ctacaaaatc aagagggaca tccctttgcc agctatcaat tggaaattac
     8101 ttggggctag gctcatgtct ttttcaaatt tagctatcta tcttcttgga tcggcttcct
     8161 agtgtggtag attaggctaa tttttggtat aataaaagtt atggaaatcg aaaaaaccaa
     8221 tcgtatgaat gcgctctttg aattttatgc ggcgcttttg acagataagc aaatgaatta
     8281 tatcgagctc tactacgctg atgattacag ccttgctgaa attgccgagg agttcggtgt
     8341 cagtcgtcag gctgtctatg acaatatcaa gcgaacagaa aagattctgg aagattatga
     8401 gatgaaattg cacatgtact cggactatat tgtccgcagt cagatttttg atcagatttt
     8461 ggagcgctat cccaaggatg actttctgca ggagcagata gaaattttaa caagcattga
     8521 taatagagaa taagaggaag aaaaatggca tttgaaagtt taacagaacg tttgcagaac
     8581 gtctttaaaa atctacgtaa aaaaggaaaa atctctgaat ctgatgtcca agaggcaacc
     8641 aaagaaattc gcttggcctt gctcgaggcc gacgttgcct tgcctgttgt aaaggacttt
     8701 atcaagaaag ttcgtgagcg tgcagtcggg catgaggtca ttgatacact taatcctgcg
     8761 caacagatta ttaaaatcgt tgatgaggaa ctgacagccg ttttaggttc tgatacggca
     8821 gaaattatca agtcacctaa gattccaacc atcatcatga tggttggttt acaaggggct
     8881 ggtaaaacaa cctttgctgg taaattggcc aacaaactca agaaagaaga aaatgctcgt
     8941 cctttgatga ttgcggcgga tatttatcgt ccagctgcca ttgaccagct taagaccttg
     9001 ggacaacaga ttgatgtgcc tgtctttgca cttggaacag aagtaccagc tgttgagatt
     9061 gtacgtcaag gtttggagca agcccaaact aatcataacg actatgtctt gattgatact
     9121 gcgggtcgtt tgcagattga tgagctcctc atgaatgagc ttcgtgatgt gaaagcattg
     9181 gctcaaccaa atgaaatctt gcttgtcgtt gatgctatga ttggtcagga agcagccaat
     9241 gttgcgcgtg agtttaatgc tcagttggaa gtgactgggg tcatccttac caagattgat
     9301 ggcgatactc gtggtggtgc tgctctgtct gttcgtcaca ttactggaaa accaatcaag
     9361 ttcactggta caggtgaaaa gattacggac attgaaacct tccacccaga ccgcatgtct
     9421 agccgtatcc ttggtatggg ggatatgctc actttgattg agaaagcttc tcaggaatac
     9481 gatgaacaaa aagcccttga aatggctgag aagatgcgcg aaaacacctt tgattttaat
     9541 gatttcatcg atcaattaga tcaggtgcaa aatatggggc cgatggaaga cttgctcaag
     9601 atgattccag gtatggccaa caatccagcc cttcaaaaca tgaaggtgga tgaacgccag
     9661 attgctcgta aacgtgcgat tgtgtcttcg atgacacctg aagaacgtga aaacccagat
     9721 ttgttaaatc caagccgtcg ccgtcgtatt gctgctggtt ctggaaatac attcgtcgaa
     9781 gtcaataaat tcatcaagga ctttaaccag gctaaacagc tcatgcaggg tgttatgtct
     9841 ggggatatga ataaaatgat gaagcaaatg gggattaatc caaataacct tcctaaaaat
     9901 atgccaaata tgggaggaat ggatatgtct gcccttgaag gaatgatggg acaaggcggt
     9961 atgcctgact tatcagctct cggaggagca ggaatgccag atatgagcca gatgtttggt
    10021 ggcggtttga aaggtaaaat tggtgaattt gccatgaaac agtccatgaa acgtatggct
    10081 aacaaaatga agaaagcgaa gaagaaacgc aagtaagaca agagattgat gcaaatctca
    10141 tttgatgaga aaataaagta aaaatttcta tgataaaacg tacagtatca aggttttaga
    10201 atatctgata ttatacgttt tttgatttta ataattttgt taagacactt ttattatggt
    10261 atacgaaaaa tccactcagc tgtctgagtg gattaggtgt atttagactc ttagtcttta
    10321 ttttcgtatg gcaagatcaa gttcaagatg attcctgtca tggctgaaag ggcagtacct
    10381 gaaagtgtaa ctggaccaag tttaaggata gctcctccaa gtccaagaac caacatagca
    10441 cttgcgatga tgaggtttcg catttgagcg aaatcaacac gttctttaat caagactttc
    10501 aaaccattgc tggcgataac cccatagaga aggattgaca taccaccaag tacagcgttt
    10561 ggaatagttg aaatcaaggc agtgaattta ccaaggaagc tgagggcaat cgcgatgaag
    10621 gcagcgttac ggataactga gacagaagcg atacgagtca taccgataac ccctgtattt
    10681 tctccgtaag ttgtattggc tggtccacca aggaaggcag aaacagaagt tgcgataccg
    10741 tcaccaagaa gagtacggtg aagacctggt tcttttaaga attgacgacc acagatttga
    10801 cccaaaacag tatggtctcc gatatgttca gaaattgtta cgatagcgat tggcaagata
    10861 gcgatggctt ctggaccaaa gtaaagattg tactctttaa aggcaccacc tgtgctaaat
    10921 ggcaagtaga aaccaggaat ttcgaaccag ttggctttaa gaactggtat aaagtcaacc
    10981 aagccaagag ttagtgcgaa aaggtaacca ccgataatgg caaagaggaa tggaatgatt
    11041 cgtaggaagc cttttccttt tgtattgata aaggcagcaa ttaggaaagt aacaacggct
    11101 accagagcat ttttccaatt tccgtctgct acaagacctg cattggtaac agctgaacct
    11161 gcaagtccaa gaccgataac gatgatcata ggaccgataa tgattggtgg caagagttta
    11221 tcaatccatt ttgttcctac aaatcggatg ctggtagcaa caaggacata gaccaaacca
    11281 gtcaagataa cccctgtttg ggcagcagat acatcacccc ccatttcttt catagccagt
    11341 gacatagctg tgataaaggc aaatgaagaa cctagataaa ctggaacttt aaaaccagta
    11401 gcaatcatgt agatgagtgt tccaacacct gaagcaaaaa gggcaacaga tacaggcatt
    11461 cccaaaatca atggtaccaa gatggtcgca ccaaacatgg cgaaaacgtg ttggaagcta
    11521 aggagaattc cttttccagc cgaaggacgt tggtcaacgt ctaataacaa atcaacagtt
    11581 gattcttgtt tcatataaac ctcacttttg ggcatcagaa aagagcccat tattttacag
    11641 caataggccc tcagagtaag acttctctaa aatctagact ttaaagagtt tcaaatattt
    11701 ttgcgtcttc gtcacctcac gggatgacat taaaaacctt tgcttaggat agtatagcag
    11761 aaaaaaatga ttttgtaaat cattttttcc cgagcctaga aataaaagag cgaggctgat
    11821 tttgtaaatc attttttccc gagcctagaa ataaaagagc gaggttgatt ttgtaaatat
    11881 ttttttactt aaaaatttaa agcgggcgtt tatttggcat accagcctta cgtggatatt
    11941 tatttggtgt ttcttttttc ttttctacca ctgtgatgta acgcggatct ctatttggta
    12001 gggcgtagct gagattgtct tcgaccttac taaaaaggag attgagggca ttcttagctt
    12061 ctaacaactc ctcaggcgca ttgctggcct tgagtgccaa tagttttcca ccaaccttaa
    12121 ggtagggaat agtcaattca gataggacct gcatacgggc aaccgcacga gctgttacaa
    12181 aatcatattg agcacggaag ttcttgtctt gggcaaaatc ttcggcacgt ccgtggtaga
    12241 aatgaactcc gttcaaatcc agttcttgag ccaagagttg taggaagttg atgcgcttat
    12301 tgagtgaatc aataatggtc acatctaact ccggatagag aattttcatt ggtagactag
    12361 gaaatcctgc cccagccccg atatcaagaa gtttgatagt ttcattggga atcaaacctt
    12421 gaagaatggg tgcaatcgaa tcgtaaaaat gtttgagata aacttcttcc ttgtccgtaa
    12481 tcgccgtcaa attaatcttc tcattccact cgaccaagag ctcaaaataa cgttcaaatt
    12541 gttctttttg ctggtccgaa agtggaagat tctgctcggc aagcaagttg taaaatgttt
    12601 ctggtttcat agttatttcc ttctttagta tcatcttatt ttaccacaat cattaaaaat
    12661 ttgatatact ggtactaatc taaaagtaga aaggacttat atcatgactt ggattattct
    12721 tggagttatc gctcttattg ttatttttgt gattgttagc tataacggtt tggttaagaa
    12781 tcgtatgcaa accaaggagg cttggagtca gattgatgtt cagttgaaac gtcgaaatga
    12841 cctcttgcca aacttgattg agactgtaaa aggttatgcc aaatatgaag gttctaccct
    12901 tgaaaaggtg gcagaactac gtaaccaagt ggcggcagcg acttcaccag cagaagctat
    12961 gaaagccagt gatgccctca ctcgtcaagt ttcaggtatt tttgcagttg cagaaagcta
    13021 tccagatttg aaagctagtg ctaactttgt taaattgcaa gaggagttga caaacacaga
    13081 aaataaaatt tcttactctc gtcaactcta taacagtgtt gtcagcaact acaatgtaaa
    13141 attagaaact ttcccgagca atattatcgc tggaatgttt ggatttaaag cggcagattt
    13201 ccttcaaaca cctgaagagg aaaagtcggt tcctaaagtt gattttagcg gtttaggtga
    13261 ctaagatgtt gtttgatcaa attgcaagca ataaacgaaa aacctggatt ttgttgctgg
    13321 tatttttcct actcttagct cttgttggtt atgcggttgg ttatctcttt ataagatctg
    13381 gacttggtgg tttggttatc gcactgatta tcggctttat ctacgctttg tctatgattt
    13441 ttcaatcgac agagattgtc atgtccatga atggagcgcg tgaggtggat gagcaaacgg
    13501 caccagacct ctaccatgta gtggaagata tggctctggt cgctcagatt cctatgcccc
    13561 gtgttttcat cattgatgat ccagccttaa atgcctttgc gacaggttct aatcctcaaa
    13621 atgcggctgt tgctgcgact tcaggtctac tagctatcat gaatcgtgaa gaactagaag
    13681 ctgttatggg acatgaagtc agtcatattc gtaattatga tatccgtatt tcgactattg
    13741 cagttgccct tgctagtgct atcaccatgc tttctagtat ggcaggtcgt atgatgtggt
    13801 ggggtggagc aggtcgcaga cgaagtgatg atgaccgaga tggaaatggt cttgaaatca
    13861 ttatgctagt ggtttcccta ctagctattg tactggcacc tctcgctgca accttggttc
    13921 agctcgctat ttctcgtcag agggaatttc tggcagatgc atctagtgtc gagctgactc
    13981 gcaatcccca gggaatgatt aatgccctag ataagttgga caatagcaaa cctatgagtc
    14041 gccacgtcga tgatgctagc agtgcccttt atatcaatga tcccaagaaa ggtggggggt
    14101 tccaaaaact cttttatacc cacccaccta tctcagaacg gattgaacgt ttaaaacata
    14161 tgtaaaatga aggctggaaa aaagtcttta aaatctgaaa aacgcataat atcaggtgtg
    14221 aaaacttgat attatgcgtt ttactatggg aagatttact tctttttctc ctaaaattgt
    14281 gtttttgccc cacctatctg ctatgttgca aatttataaa tcttctaaat taactagctt
    14341 caaaccgtgt tctgtcatgc gatagatgat cgagcagact tcttttaaga aacgacggtc
    14401 atgcgaaaca gtgatgagac cgccaggata agtggcaaag agttttctga tttggggttg
    14461 agaagtggga gaaaagtttc gtgtgggttc atccagcagg agaaagtttg gtttgcgcag
    14521 gactaaatcc aaaagcagga gttttccctg ttgtccgcca gataaggagc gaatttgatg
    14581 ctgcatttct ggataactga aattgagact agctaggtga gattggattt tctgtagttc
    14641 ctctttttcc ccagttttac tgagataggc tattggggat aaatccaatt gcagtttttt
    14701 gtggtaatct tgtggcataa aaccaagtga aatctctctt ttatcattca gaagtctctg
    14761 taacttggct aacagagttg atttcccaac accatttggc ccgataatac cgattttttc
    14821 ttggccacgg acagttagtt gtagtttttg aaccaaaact cggtcgtcaa tggacaaatt
    14881 ttctttttcc agttggacta agactttaga agctggtaat ggttggatgt ctgaaaagaa
    14941 aagttggatt tgttcctctt caagtggttt ttgagtcatg gactgagctg ccttttcgta
    15001 gcgtttttct tgtgagagga cagttttcat ctttttagcc aataggcgac cggcagtact
    15061 atctttggta gctcgaagcg cagtttctac attttgctta actctccgat gtttttccat
    15121 ggttttatcg taggctcttt ggttgttagc agcttgctga ctttgtttgg caaaattagc
    15181 ctttctctgc tcactatagc tatcatagtc taaatgctct actagcgttt ccgcttcttt
    15241 acggtgtttg accagtcgca agtgaacaat agtgtctgcc gtttcagaaa gaaagtcttc
    15301 atcatgggaa atgaaaataa cggtttgcct ggtcttttga atctggcctt ttagccaatc
    15361 aactgtctca aggtctaggt catttgaagg ttcatctaaa aatagaatct caaagggttt
    15421 ggctaactca tggataagct gaattttcaa agcttctccc cctgatagat tgccaatctc
    15481 ttggtcactt gcgaaacgat tgctatcaaa atgcaattcc tccgccaaac gatagaggat
    15541 actgtagtct aaatcaatag aatctaaaaa gaagtagtcg tgtaaagttt tctttttcag
    15601 ctcctcggga agtttttgag gaatgtaggc cagtgactga tagtcagatt ggatgtctcc
    15661 cttgatagtg aaatcagaca aatcttcccc cattaaagtt ttaagtaagg ttgatttacc
    15721 atttccttct tcgccaataa tagctacctt ttccccgtct tgtatggtca tgcttaggtc
    15781 agagacaagg tctcgtaaat ctttgttttg tgtgatggtc aggtgattga tttttatcat
    15841 actattgccc tttctagaat gtcgatagtg cataacaaaa agctctactt tacctagtag
    15901 agcccaaagt ctatgtcaaa aaaactctat taacatgcca gaaaaagagc ataaaagagc
    15961 acacaaaaag agacaaaaac aagatctact aaataaaggt tctttatttg atagacttag
    16021 ttgttcatgt ctcccttacc tccgagtatt agtatcttta tattatacct ttaaggaaat
    16081 tttgtcaaca gaaaactgga aattctatgc tctaaaatcc aaaaacaggt ccatagagac
    16141 ttagtacgtg tttgacatac tcgtaatgga ttttatcata gtttcgccaa gcagtgcgtg
    16201 cttgatcgtt tagttttagg ctacccagtc caatcagaag actggtgggt tggtaaaatt
    16261 tctcaagatc aattaagata ctgttgttca acttttccgt ttttttgagc tcttgcatag
    16321 tagtgtttag cagctcttgt agtcgaatat catctgccgt accctgttta cagttttggt
    16381 agcttttatt taacttggat aggagttgaa tgagtttctt gtcttccatg tgagcatcct
    16441 ccttgatctg atgtactctt tccctatagt atagcactaa aaaacaaaac tgtcatagta
    16501 aatatgttaa aattctgaat tttaattaca aagtttggtg tgctgatttg tagccttttc
    16561 atggtataat caagagagta aatctttatg gaggaagtat gaaattaaat attcaagaaa
    16621 ttcgtaagca gtctgaaggt ttgaactttg aacaaacgtt agatttagtt gatgacctgc
    16681 gtgcacgtaa tcaagaaatt ttagatgtaa aagatatcct tgcagttggg aaagtacaat
    16741 atgaagaccg tatgtatttc ttagattatc aactatctta taccattgtt cttgcttcga
    16801 gtcgcagtat ggagccagtt gagttagttg aatcttatcc agtcacggaa gttttcatgg
    16861 aaggcgcaac taaccatcta aatcaagaaa ttttagatga tgacttggtc ttgcccatcg
    16921 aaaatgaggc gcttgacctt gctgagagtg tatcagacaa tatcttgcta aacattccta
    16981 tcaaggtctt gacggctgaa gaagaagctg gtcaaggatt tgtctcagga aatgactggc
    17041 aaatcatgac agaggaagaa taccaagctc aacaagcagt aaagaaagaa gaaaacagtc
    17101 cttttgctgg cttacaagga ctatttgacg gagatgaata atggtcttgt caaaaaaacg
    17161 agcacgaaag gtgctagaag aaatcattgc cctcttccca gatgccaagc ctagtcttga
    17221 ttttaccaat cattttgaac tcctggttgc ggtcatgtta tcagcccaga cgacagatgc
    17281 agcggtaaat aaggccacac caggtctctt tgttgccttt ccaacacccc aagctatgtc
    17341 tgtagcgaca gagagtgaga ttgcttcaca tatttctcgc ttgggattgt atcggaataa
    17401 agctaaattc cttaaaaaat gtgcccaaca gttactagac gattttgatg gtcaagtccc
    17461 tcagacacgt gaagaattgg agagtttggc aggtgttggt cgcaagacag ccaatgttgt
    17521 catgagtgta ggatttggga ttccagcctt tgcagtggat actcatgtgg agcgtatttg
    17581 caaacaccac gatattgtca aaaaatcagc gacgccactt gaggtggaaa agcgggtcat
    17641 ggatatcttg ccgcctgagc agtggttagc tgcccatcag gccatgattt attttggaag
    17701 agccatttgc catccaaaaa atccagagtg tgaccagtac ccacaattat atgattttag
    17761 caatttgtaa gatgctacat tgaaactttt tgcttgattt taagcatgct ttgtgctata
    17821 gtaaatgata actaaatatt cctttaaacc tgtcccgtga ggcaggcaag gagcgcgata
    17881 aagtagaagg gtgaagtcta tactgtgtgc agtagggctc gcttggctat aaatttcaag
    17941 tctccttgtt taaggagact tttctttttg gaggtttgtc atgaaaacaa aagaagttgt
    18001 agacgaattg accgtcaaac gagcgattac gcgtattact tatgagatta tcgaacgcaa
    18061 caaagatttg aataagatcg tcttggctgg tattaaaact cgtggtgtct ttattgccca
    18121 ccgaatccaa gaacgtttga agcagttaga aaatctttca gttcctgttg tggaattgga
    18181 tactaaacct ttccgtgatg atgttaaaag tggagaagat acttctttgg tttctgtcga
    18241 tgtgacagac cgcgaagtta tcttggtgga tgatgtgctc tatacaggtc gtaccatccg
    18301 tgctgctatt gataatattg tcggtcatgg tcgtcctgcg cgtgtgagtt tagcagttct
    18361 agtcgatcgt ggacatagag aattgccaat ccgtccagat tacgttggaa aaaatatccc
    18421 aaccagtcgt tctgaagaaa tcatcgtaga gatgacagaa cttgatgacc aagacagagt
    18481 tctgattact gaagaagctt agaaagttaa aggagtagcc atgtcagaaa atcaacaagc
    18541 attgaaccat gtggtgtcca tggaagacct cactgtcgat caagtaatga aattgatcaa
    18601 gcgaggaatt gagtttaaaa atggagccca gcttccctat gaagaccatc cgattgtttc
    18661 caatctcttc tttgaggatt ctacacggac acataagtcc tttgaagtcg cagagattaa
    18721 acttggattg gaacgacttg actttgatgt gaagactagt tcggttaata agggtgagac
    18781 actttatgac accattttga ctctgtctgc tttaggagtg gatgtctgtg tgattcgcca
    18841 tccagaggtc gactactata gagaattgat tgctagtcca acgattacga cttccatcat
    18901 caatggtgga gatggttcgg gccaacaccc tagccagagc ttgcttgatt tgatgaccat
    18961 ttatgaggaa tttggccact ttgagggtct taaagttgct attgcaggtg acttggacca
    19021 ctcacgcgtt gccaaatcca atatgcagat tttgaaacgc ttgggatctg aactcttttt
    19081 cgctggacct gaggaatgga gaagtcaaga gtttgcagac tatggacagt ttgtaaccat
    19141 tgatgaaatc attgatcagg ttgatgtcat gatgtttctc cgtgtgcaac acgaacgcca
    19201 tgatagtgga gctgtatttt caaaagaaga ctaccatgcc caacatggct tgactcaaga
    19261 acgttacgat cgtttgaaag aaacagcaat cctcatgcac ccagctccaa tcaatcgtga
    19321 tgtagaaata gcagaccact tggttgaagc accaaaatca cggattgtcc aacaaatgac
    19381 caatggtgtc tttgttcgaa tggcaatctt agaatccgta ctagcgagta gaaacgccaa
    19441 ctaaaacaca agacgttaaa agacgccagt gagcgtccac gagaggtgaa aatatgacaa
    19501 aaagaattct agtattagaa gatggcacag tttttgaagg caaggccttc ggagcagata
    19561 ttgatgtaac aggcgaaatc gtctttaata cagggatgac cggctaccaa gaatccatta
    19621 cagaccagtc ttataatgga caaatcttga cctttactta tcctttggta ggaaattatg
    19681 gtattaatcg tgatgattat gaatccatta ttccaacttg taagggagtc gttgtttttg
    19741 aagaagcgcg tagagctagc aactggcgca accaaatgac cttggatgaa tttttgaaag
    19801 ccaagaaaat tccagggatt tcaggaattg atacgcgtgc tcttaccaag attatccgta
    19861 agcatggtac tatgcgtgca accttgaccc atgttgggga cagtatggac catgtgacgg
    19921 accagctcca agcaacagtt ttaccgacag acaatatcaa acaggtttct actaaaacgt
    19981 cttatccagc tccaggagtt ggtttgagcg tggtgctagt ggactttggt ctcaagcact
    20041 caatcctacg tgaactttct aaacgcaact gtaacgtgac agttgttcct tattcgacaa
    20101 cggctgaaga aattctccat ctcaatcctg acggagttat gttgtcaaat ggtccaggta
    20161 acccagaaga cgttccacaa gcactcgaca tgattcgcgg tgtacaagga aaaattccaa
    20221 tctttggtat ttgtatggga caccaactct ttgcaatggc aaacggggct aagacctata
    20281 agatgaagtt tggccaccgt ggattcaacc atgcggtacg tgaaatcgca acaggacgag
    20341 tagacttcac cagccagaac catggttatg cagtcagccg cgaggatttg ccagagcact
    20401 tgattattac ccacgaagaa atcaatgaca agtcagtgga aggtgtgcgc cacagatacc
    20461 aacctgcttt ctctgttcaa taccacccag atgcagctcc tgggccacac gacgctagct
    20521 acctatttga cgagtttatc gagatgatgg aagtttttaa acaatcaaac taagaacgag
    20581 taaaagctcg tcggagaatt tatgtaactg aacacggacg gaaagctcag aatgtagaga
    20641 aaccaacttg gattgtcaat ccttccttga tttcctaaaa ttcaatcgct ttctattcgt
    20701 cctttgtatc ttatttaatg tcgccctgag aggcgacgaa tagaaaggag aagatacatt
    20761 gttcgcggaa gtgaacacgc cctaagaact gtgtgaaaaa gataaacatc gtcttgacgc
    20821 tgaaggcgtc tgcgccgagt ttcctatttt cactgtgttc tttacgggct ttgtatcata
    20881 aactatgcct aaacgtactg atattcaaaa aattatggtg attggttctg gtccgattat
    20941 tattggtcag gctgctgagt ttgactacgc tgggactcag gcttgcttgt cgttgaaaga
    21001 ggaaggttat gaggttgttt tggttaactc aaatcctgca accatcatga cggacaagga
    21061 aattgctgac aaggtttaca ttgaaccgat tacacttgag tttgtgacac gtattcttcg
    21121 taaggaaggt ccagatgcct tgctaccaac actcggtggt cagacaggtc tcaacatggc
    21181 catggaatta tctaaaaatg gtatcctaga cgagcttggc gttgaacttc tgggtaccaa
    21241 attatctgcc attgaccaag cggaggaccg tgatctcttt aaacaattga tggaagagct
    21301 tgaacaaccc attccagaat ctgaaattgt taacacagta gaagaagcag ttgcctttgc
    21361 agcgacaatt ggctacccag tcatcgttcg tccagccttt acccttggtg gtactggtgg
    21421 tggtatgtgt gccaacgaga aagaattgcg tgaaatcact gaaaatggtt tgaaattgtc
    21481 acctgttacc caatgtttga ttgagcgttc gattgccggt ttcaaggaaa ttgaatacga
    21541 agtgatgcgt gactcagctg acaatgcctt ggttgtttgt aacatggaaa actttgaccc
    21601 agttgggatt cacacagggg attccattgt atttgcccct gcgcaaacca tgtcagacta
    21661 tgaaaaccaa atgcttcgtg atgcgagctt gagcattatt cgtgccctca agattgaagg
    21721 tggatgtaat gttcagctag ctcttgatcc taacagcttc aagtattatg ttatcgaagt
    21781 aaaccctcgt gtatcacgtt cgtcagccct tgcttctaaa gcgacaggat atccgattgc
    21841 taagttggct gccaagattg cagtaggttt gaccttggat gaggttataa acccagttac
    21901 aggttcaacc tatgccatgt ttgaaccagc ccttgactac gtagttgcta agattccacg
    21961 tttcccattt gacaagtttg aaaagggtga acgccgtctc ggtacacaaa tgaaggcgac
    22021 tggagaagtc atggcaatcg gtcgtaacat cgaggaatct cttctcaagg cctgccgctc
    22081 ccttgaaatt ggggtgcacc acaatgaaat acctgaactt gcagcagttt ctgatgatac
    22141 cttgattgaa aaggttgtca aagcccaaga tgaccgtctc ttctacgtat cagaagccat
    22201 tcgtcgtggc tacacacctg aagaaattgc agagcttaca aaaattgata tcttctatct
    22261 tgataaactc ttgcatatct ttgaaattga gcaagaattg ggtgctcatc cacaagatct
    22321 agaagttttg aaaacagcca aacttaatgg tttttcagac cgtaagattg ctgaactctg
    22381 gggaacgact gatgataaag ttcgccaact tcgcttggaa aacaagattg ttccagttta
    22441 taagatggta gacacttgtg cggcagagtt cgattccgaa acgccatatt tctattcaac
    22501 ctatggttgg gaaaatgaat ctatcaaatc tgataaagaa tctgtacttg tcctaggttc
    22561 aggtccaatc cgtatcggtc aaggggttga gtttgactac gcaaccgttc actctgttaa
    22621 ggctatccag gcagctggtt atgaagctat catcatgaac tcaaacccag agaccgtttc
    22681 aacagacttc tctgtatcag ataagcttta ctttgagcca ttgacattcg aagatgttat
    22741 gaacgtcatt gacttggaac aaccaaaggg agttatcgtt cagttcggtg gtcaaacagc
    22801 catcaacctt gcggagccat tggcaaaagc aggtgtgacc atccttggta cacaggttgc
    22861 tgacctagac cgtgccgaag accgtgacct cttcgagcaa gctctcaaag aattggatat
    22921 tccacagcca ccaggacaaa cggctaccaa tgaagaagaa gcagctcttg cagctcgtaa
    22981 gattggcttc ccagttcttg tccgcccatc ttatgtactt ggtggacgtg ccatggaaat
    23041 cgttgaaaac gaagaagacc ttcgttctta catgcgaact gctgttaagg ctagtccaga
    23101 ccatccagtt cttgtcgact cttatatcgt tgggcaagag tgcgaagttg atgccatttc
    23161 agatggtaaa aatgtactca ttcctggtat catggagcat atcgaacgtg ctggtgtcca
    23221 ctcaggtgac tcaatggctg tttacccacc acaaaccttg tcgcaaaagg ttcaggagac
    23281 catcgcagac tacaccaaac gcttagcaat cggtcttcac tgccttggaa tgatgaacat
    23341 ccagtttgtt atcaaggatg aaaaagtcta cgttattgag gtcaatccac gtgccagccg
    23401 taccgttcca ttcctatcta aggtaaccaa tattcctatg gctcaagtag cgaccaagct
    23461 cattcttggt caaagtttat cagaacttgg ctaccaaaat ggactttacc ctgaaagcac
    23521 ccgtgttcat atcaaggcac ctgtcttctc ctttaccaaa ctagctaagg tagacagctt
    23581 gctcggtcct gaaatgaagt caacaggtga agttatgggt tctgatgcta ctttggaaaa
    23641 agctctctat aaagcctttg aagcttctta tctacacttg ccaacttttg gtaatgtcgt
    23701 ctttaccatc gctgatgatg ccaaagaaga agccttgaac ttggctcgtc gtttccaaaa
    23761 cattggttat ggaatccttg cgacagaagg gacagcagcc ttctttgcta gtcatggctt
    23821 gcaagcccaa cctgttggta agattggtga tgacgataag gatatcccaa gttttgtacg
    23881 caaaggaaga attcaagcta tcattaacac agtaggaaca aaacgaactg ctgacgaaga
    23941 tggtgagcaa attcgtcgtt cagccattga acacggggtg cccctcttca cagcgctaga
    24001 tacagctaat gccatgctta aggtacttga aagtcgtagc tttgttacag aagcgattta
    24061 aatagaaaaa taagatatag cagactgaat ttagaacagt acacaggaat actaaaatat
    24121 ttctataaat taatttgaat tttctaattg atttgttcgc atcttatttc aatctactat
    24181 agaaaaaagt ctttaaaatt ataaaacgca tcatatcaag gtttttcaaa aaccttgata
    24241 tgatgcgttt tattgtggga atatgtattt cattttctac taaaattatg tttttgaata
    24301 acctctatct tagtagtttg tataatcccc ctcaatcagt ttttgcgata agctttaatg
    24361 ctatgactat accactcttg catttctttt gatggtggtg tcatataatc gccatacatc
    24421 tgggttaaaa attggtcata ttttttggga acaggcaaca tacggccctc aaactcagtt
    24481 aaaatcagtt ctttaaaggt atcaactggg aagatttctt tcatcccttc cttaccgatc
    24541 ccaactcctc cttcatattg aggaatgttg gttacagcat ttttgactag ttgatcaatt
    24601 ttcttgtaaa agtagcgagg attgacaaat cggagagcgt accagctaca taatctaaga
    24661 aaatctttta gtttgctatc accgtgaact gctcgtgatt ttttgatata agctagttga
    24721 cgaagagcca catacttata gctcttgtcg acaatgctca agtctgtaaa tcgatcaatt
    24781 gggaagacat cgatgaaaag gctggtatca tgacgcttgt acttaacatg gtcttctata
    24841 acagtagaag tgtccaaaat cgatgcgaaa ttatggaagt accaagaaga tgtatcgtag
    24901 gaaagaacct tgtagcgagg gtgattttct tcttcaataa tcttcagtaa acgctcataa
    24961 tcctcacgat aaagggaaat atcaatatca tcatcccaag gaatcatacc tttgtggcgg
    25021 atggctccaa gcatggttcc ataactgaga aaataaggaa tatcatgttt cttacaagtc
    25081 tcatcaatat agtccagcag ggctagttga atttctttaa tttctttttt ttctaaatat
    25141 tgcatcctaa tcctccaatt tataagcgtg aaattcatga ctgtagaagc gtttttcttc
    25201 tggtggtagg gtcatataat ctccataaaa ttgtgtcaaa atagtatcaa atttttcagg
    25261 tgcaggaagg cttaaattct caaagggtaa atcgattgtt ttatcaaagg taccacttgg
    25321 gaagacttcc ttttccttaa attttgaagg gataaaagcc atatactgcc cattttcacg
    25381 actatatttt tgaatttctt tctcgatttt atttgcaaaa taacgaggag aaactggtcg
    25441 aaggagcaac caaaaggctg ttcgtatcca atcttttaaa aggctatcct tatagacaat
    25501 atttttatgt ttactgaaag acagcagttt gaagctttcc agtttataac aagtatcaat
    25561 gaccttagga tcatcaaagc gatccatagg gaaaatatcg ataaagatac cagactcata
    25621 ggtttttgta tttcgagtat caataatttt agtcgtactg tcggttatct tgataaagtt
    25681 gttaaagtag ttcttatcag tttctaagga taggagttca tacttgcttt tttccttttg
    25741 aaaaatgtta ataaatcgtt ggtagtcttc tctaggcatg gataaatcaa tatcgtcgtc
    25801 ccaagggata aagccctcat gtcgaaccgc cccaatcaga gtaccgtagt taataatata
    25861 gttgatattg tgctttttac agagagtatc aatataatcc aaaatttcta attcaatttg
    25921 tttggcatct tcaacggtta gttgtttcat tttaaactcc tatgattttt taaatttatt
    25981 ttttaaggct agaacatggt ttaaaaattc atagaaaatg ctatcttttg tgaagacaag
    26041 tagactaata taagagatag ctgatagcaa gataatcaaa ccagtattaa tcaaaaatgg
    26101 caaattaatg accatatcca caggatacac gaaattaatc aggaaataaa ttgctacaaa
    26161 ggaaagtgaa aagagagagt atcgaacagt atagctaaag atatgtccca agtggatgag
    26221 ttgttttcta tggatgaaaa tgatatagaa aacaagtaga gaagcctctg ataacatagt
    26281 tgtcagtaag tagtattcgg gagccacgat acggttgaaa aagaggagac tattcaagcc
    26341 caaattgagt agcccagcaa agactgtata aactgtgata cgtttttcat agccattggt
    26401 aaagagaatt tgggaaccaa gaatggtatc taaggccagg ataatcgtac gaaaagcgaa
    26461 gagagaggtc aagatgccgc ctccgatata tttttcacta ccgtaaagta ggatggcatt
    26521 tggtcctaaa accatgagtc caaaactcag tggaatgata aagaagttaa agattcgact
    26581 acctctatta accagagaaa cataggcttc tttgtctcct ttccccagat agtaactgag
    26641 acgaggcaca ctcactccaa ttgcacctgt tacaacccca gctataacgg tcacaattcg
    26701 ctgagctatg gtatagtaac taacgttgac atcaatccct gttttaacga ggaagaggcg
    26761 atctaaaaaa gtgaagagca tattggcatt ggcaaagact aacatggctg tcagagggag
    26821 aaagagtggt ttaaaatcac ttaggtgaat cttaacaagt ttgatgtctc ttttaatcca
    26881 aaaataacta atcaggtagt taatcagcgt cgataaactc atcacaagtg tatagacaac
    26941 aatatcgtgt tcatttttaa caaataagaa aatagagacc agcatcagga tacggatgaa
    27001 ggcagttttg taaaagagaa aactgtaatt ttccagagct tcattgaccc attcgattga
    27061 aaaaatctgg gcaatgagtt gaatccccat aacaaggtag acctttttga tgattggatt
    27121 atcagtaaag aagagaggat aggctaggat atagacagca gtggtcaaaa tcgtacaagc
    27181 gatgcacaaa taaaaaagac tagaaaaggt tctgttaaga tcttttttgt tatccttgac
    27241 attactgata gcccttaaac cgtagttata gacaccataa gttgcaaagg gcaagaaaaa
    27301 tgacaaaata gtgtcgactg agttgaagta accatagtca gttcggtcca agacacgcgc
    27361 gacataggtt ccagttagga tgggaaaaat aatattcaag acacgaattc ccatgtaaga
    27421 tagagcattt aattttatac ttttcattca atttacctcg tttttcatta tatcataaag
    27481 ttagctaata agaaatgaag ggcagtaagt caagtaatca ctttgaagtt tcaaatctta
    27541 agttttaagt tttctttaag gaaagtatat tattctgaag gactctaaaa tttcgcagcc
    27601 atttattagt aattgctaca gaattcctag tcattactag aaatggacta gtttctttga
    27661 ataatagaac tgcataattc tcctattcta gaaggggagg accagtattt cttttatgat
    27721 aggactagat tgtggtataa tagagagaat aagttttttt agtaagacaa aggagaaaat
    27781 agatgattta tgcaggaatt cttgccggtg gaactggcac acgcatgggg atcagtaact
    27841 tgccaaaaca atttttagag ctaggtgatc gacctatttt gattcataca attgaaaaat
    27901 ttgtcttgga gccaagtatt gaaaaaattg tagttggtgt tcatggagac tgggtttctc
    27961 atgcagaaga tcttgtagat aaatatcttc ctctttataa ggaacgtatc atcattacaa
    28021 agggtggtgc tgaccgcaat acaagtatta agaacatcat tgaagccatt gatgcttatc
    28081 gtccgcttac tccagaggat atcgttgtta cccacgattc tgttcgtcca tttattacac
    28141 ttcgcatgat tcaggacaat atccaacttg cccaaaatca tgacgcagtg gacacagtgg
    28201 tagaagcggt tgatactatc gttgaaagta ccaatggtca atttattaca gatattccaa
    28261 atcgtgctca cctttatcaa ggacaaacac ctcaaacatt ccgttgcaag gacttcatgg
    28321 acctttatgg atctctttct gatgaagaga aggaaatctt gacagatgca tgtaaaatct
    28381 ttgtgatcaa aggaaaagat gtggctttgg ccaaaggtga atactcaaat ctgaagatta
    28441 caaccgtaac agatttgaag attgcaaaaa gtatgattga gaaagactag taaaatgatt
    28501 aatcaaattt atcaactaac taagcctaag tttatcaatg tcaaatatca ggaagaggct
    28561 attgaccaag agaatcatat ccttatccgt cccaactaca tggctgtctg tcatgcggat
    28621 cagcgttact atcagggaaa acgtgatccc aagattttga ataaaaagct tccaatggca
    28681 atgattcacg agtcatgtgg aaccgtcatt tctgacccga ccggaaccta cgaggttggt
    28741 caaaaagttg tcatgattcc caatcagtct cctatgcaga gtgatgaaga attctatgaa
    28801 aactacatga cagggaccca tttcttgtct agtggatttg atggctttat gagagagttt
    28861 gtttctctcc ctaaagatcg tgtggtggct tatgatgcta ttgaagatac ggttgcagcc
    28921 attacagagt ttgtcagtgt gggcatgcac gctatgaatc gtctattgac tcttgctcat
    28981 agcaagcggg agcggatcgc cgttattgga gatggaagtt tagcttttgt ggttgccaat
    29041 attatcaact atactttgcc agaagcagag attgtggtta ttggtcgtca ttgggaaaag
    29101 ttggaactct tctcatttgc caaagaatgc tatattacgg ataatattcc tgaagatttg
    29161 gcctttgacc atgcttttga atgttgtggt ggtgatggta ctggaccagc tattaatgac
    29221 ttgattcgct acattcgtcc tcagggaacg attctcatga tgggagttag cgaatataaa
    29281 gtcaatctca atactcgcga tgccttagaa aagggcttgc tcttggttgg gtcatctcgt
    29341 tctggtcgca ttgattttga aaatgctatc caaatgatgg aagtcaagaa atttgccaat
    29401 cgtcttaaaa atatccttta tctagaagaa cctgtaagag aaattaaaga tattcatcgt
    29461 gtctttgcaa ccgatttaaa cacagccttt aaaacagtgt ttaagtggga agtataagta
    29521 ctggaggtta attgtggaga aaatcattaa agaaaaaatt tcttccttac ttagtcaaga
    29581 agaggaagtc ctcagtgttg aacaactggg tggaatgacc aatcaaaact atttggccaa
    29641 aacaacaaat aagcaataca ttgttaaatt ctttggtaaa gggacagaaa agcttatcaa
    29701 tcgacaagat gaaaagtaca atcttgaact actaaaggat ttaggcttag atgtaaaaaa
    29761 ttatcttttt gatattgaag ctggtatcaa agtaaatgag tatatcggat ctgcgattac
    29821 gcttgattca acgtcaatca agaccaagtt cgacaaaatt gctccaatat tacaaactat
    29881 tcatacgtct gctaaggaat taagaggaga atttgctcct tttgaagaaa tcaaaaaata
    29941 cgaatccttg attgaagaac aaattcctta tgccaactat gaatctgtta gaaatgcagt
    30001 cttctcctta gagaaaagac tggctgactt aggtgttgac agaaaatctt gtcatatcga
    30061 tttggtgcct gaaaacttta tcgaatcacc tcaaggacga ctttatttga ttgactggga
    30121 atattcatca atgaatgatc caatgtggga tttggctgcc ctctttttag agtctgaatt
    30181 cacttcccaa gaggaagaaa ctttcttatc tcactatgag agtgaccaaa caccggtttc
    30241 tcatgaaaag attgctattt ataaaatttt acaagatact atttggagtc tatggactgt
    30301 atataaggaa gagcaaggtg cagattttgg tgactatggt gtgaatcgtt accaaagagc
    30361 tgttaaaggt ttggcttctt atggaggatc agatgaaaag taaaaacgga gttccttttg
    30421 gccttctctc aggtattttc tggggcttgg gtctaacggt tagtgcttat atcttttcga
    30481 tttttacaga tttgtcaccc tttgtggtgg ctgcaactca tgattttttg agcatcttta
    30541 tcttactagc ttttctcttg gtaaaagaag ggaaagttcg cctctcaatt ttcttaaata
    30601 ttcgcaatgt cagtgttatc atcggagcct tgctagcagg ccctatcggt atgcaggcca
    30661 atctttatgc agttaagtat atcggaagtt ctttagcttc atctgtatcg gctatttacc
    30721 ctgcgatttc agttctattg gctttcttct ttttgaagca caagatttcg aaaaatactg
    30781 tatttgggat tgtcttgatt attggaggga ttattgctca gacctataag gttgaacagg
    30841 ttaattcttt ctacattggg attctttgtg ctttggtttg tgctattgca tggggaagtg
    30901 agagtgttct tagctctttt gccatggaaa gtgaattgag tgaaatcgaa gccctcttaa
    30961 tccgtcaagt aacttcgttc ttgtcctatc ttgtgattgt gctcttctct catcagtcat
    31021 ttactgcagt agccaatgga caattgctag gtctcatgat tgtttttgca gcctttgata
    31081 tgatttccta cttggcttat tatatcgcta tcaatcgctt gcaaccagcc aaggctacag
    31141 gcttgaacgt gagctatgta gtatggacgg tcttgtttgc agttgttttc ttgggtgcac
    31201 cgctagatat gctgaccatt atgacgtcac ttgtcgtcat tgctggagtt tatattatta
    31261 ttaaagaata aaggagattc gtgtgaaagc cattatctta gcagcgggat tgggaactcg
    31321 cttgcgtcct atgactgaaa atacccctaa agccttggtt caggttaatc aaaaaccttt
    31381 gattgagtac caaattgagt ttctcaaaga aaaaggaatc aatgacatca tcatcattgt
    31441 tggttatctt aaagaacaat tcgattactt gaaagagaaa tacggtgttc gtctcgtttt
    31501 caatgataaa tacgctgact acaataactt ttactctctc tatcttgtaa aagaagaatt
    31561 ggccaacagc tatgttattg atgctgacaa ttatctcttt aaaaatatgt tccgcaatga
    31621 tttgacacgt tcgacttatt ttagtgttta tcgtgaagat tgtaccaacg aatggttctt
    31681 ggtttatgga gatgactaca aggttcaaga cattattgtt gatagcaagg caggtcgcat
    31741 ccttagtggt gtatccttct gggatgctcc aactgcagaa aagattgtca gctttatcga
    31801 caaggcttat gtaagtggtg aatttgttga tctctattgg gacaatatgg ttaaggataa
    31861 tatcaaagag ctagatgtct atgttgaaga attagaaggc aatagcattt atgagatcga
    31921 tagtgtccaa gactatcgta aattagaaga aattcttaaa aacgaaaatt aaagattcca
    31981 acatctgaca aaatagtcgg atgttttttg attttttacg aacttttacg aatagataga
    32041 tgagtagaaa aagaaatgga gttatttatg aaaatcacaa actatgaaat ctataagtta
    32101 aaaaaatcag gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg tgaaaatgtt
    32161 gatcaggagc ttttgttggg tgatattgca gatatctcag gttgccgtaa tccagccgtt
    32221 tttatggaac gttattttca gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt
    32281 ccatctttct ctattttaga tgactgttat ccttgggatt tgagtgaaat atatgatgcg
    32341 cctgtacttt tattttacaa gggaaatctt gacctcctga aattcccgaa ggtagcggtc
    32401 gtgggcagtc gtgcttgtag caaacaggga gctaagtcag ttgaaaaagt cattcaaggc
    32461 ttggaaaatg aactggttat tgtcagtggt ctggccaagg gcattgacac agcagctcat
    32521 atggcagctc ttcagaatgg cggaaaaacc attgcagtga ttggaacagg actggatgtg
    32581 ttttatccta aagccaataa acgcttgcaa gactacatcg gcaatgacca tctggttcta
    32641 agtgaatatg gacctggtga acaacctctg aaatttcatt ttcctgcccg taatcgcatc
    32701 attgctggac tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc aggtagtctc
    32761 attacgtgtg agcgagcaat ggaagaagga cgcgatgtct ttgctattcc tggtagcatt
    32821 ttagatggac tatcagacgg ttgccatcat ttgattcaag aaggagcaaa attagtcacc
    32881 agtgggcaag atgttcttgc ggaatttgaa ttttaaaaat gacctaagct agaattctaa
    32941 gaaaaaatca attttaagag aaaatgaacc caacatttcc ataataaaac gcatattagc
    33001 aagtttttaa cacttgataa tatgcgtttt ttctaagtgg attagtagag tagaggattt
    33061 ttctcatata atactcttcg aaaatctctt caaactacgt cagcttccat ctgcaacctc
    33121 aaaacagtat tttgagctga cttcgtcagt cttatctaca acctcaaagc agtgctttga
    33181 gcaacctgtg gctagcttcc tagtttgcgc tttgattttc attgagtata agggaaagta
    33241 tagtgaattg aaataagatg tgaacaactc tatcaggaaa gtcaaattga tttatagaaa
    33301 tattttagca gccaaggtgc actgttatag attcaataca ctataattta gtgtaattga
    33361 gaaaggagaa atgattgtga ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct
    33421 caaatcttgt cagtaaggaa aaataaattc ttcaaaagta gagattacaa ggcttgttta
    33481 agaaagaatt caaagacctt gacaaataaa aataaaatgg ttattataaa aaatggtctg
    33541 aaatagatga tgatactttt cgaaaatctc ttcaaactac gtcagctcag ctttgccttg
    33601 ctgtgttttg agcaagctac ggttagcttc cgagtttgat tttcatttac tagaaatgaa
    33661 actgatgaga gatatcagta gacatttgag tcaggatatt atggaaaatg ataaaaagag
    33721 ctcgtgagat tggcatatca gactactaaa gtattgagtt tgttaggatt ttagcgacta
    33781 gttagctggg aaaggaagat atttgtgaca aataataaac tgtattcgtt gatagaattt
    33841 agaaataaaa tatatgaaga attagaactt tccagaagtg atttagcgat tttactatgt
    33901 gccatgctta tcgcctctat cggattaaat atggattcga ctcccgtgat tattggagcc
    33961 atgttaatct ctcctttgat gacacctatt ctgggagtgg ggctctctct agctatattt
    34021 gattttaaat tgttaagaaa atcttttaaa atattagcta ttcaaattct tgccagtcta
    34081 atagcttcaa cactttattt ttatctttct cccatttcgt atgctagttc ggagattgtt
    34141 gctagaacct ctccgactat ttgggatgtt ctcattgctt ttgtaggagg gatagcaggt
    34201 attattggtg ctaggaaaaa agagaccaat aatattgttc ctggtgttgc tattgcaacc
    34261 gccttgatgc ctcctctttg tacagtaggt tatgctattg cttctgctaa tctaaaattt
    34321 atcataggct cctcttacct attcctcatc aattgtagct ttattgtcat tgcgacttat
    34381 ataggtgtta ggttgatgat ggttaagaaa cattatttta aagataatga agaagactct
    34441 aaaatgcgta ggattttgct tctagttgct gttttgctga tgattccgag tttcatctct
    34501 gcaacgactt tagtgagaga aacgttgaaa aaagagtccc ttaagaaatt tatatcagag
    34561 cagtttcagg ggtataatat tttgaaaaaa acctattcta aaaagactca taccctaaag
    34621 ctaaccattt caggaaatta tttgacagaa gaagaactcg atatgatttc aagtaagaga
    34681 ggtgactatg gtttaagtga tgtttctgtt caagtttcac aattgtctga ttcagaacaa
    34741 cttagtaagg aagagctggt ggagtatttc ttccagtata tcaaggataa ggaagcaaaa
    34801 gaaaaggaga aagccaataa atttgataca gagtctgagg agcaataatt tcttgagaat
    34861 agctggtttc tctcgtgagt cttctatgta tatcaaagga agactgaggt tttaagtata
    34921 aaacttttct tctattatag tagctaagtc ttgacagggt tcaaaaaatg gtttacactt
    34981 tataaagttt attactttga aaaggtgtga tactgtggct acggcaacaa aaaagaaaaa
    35041 atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct gctaaggcca agacgattga
    35101 aaaatatcta ggcagaaact acaaggtttt agccagtgtc gggcatatcc gtgatttgaa
    35161 gaaatccagt atgtccgtcg atattgaaaa taattatgaa ccgcaatata ttaatatccg
    35221 aggaaaaggc cctcttatca atgacttgaa aaaagaagct aaaaaagcta ataaagtttt
    35281 tctcgcgagt gacccggacc gtgaaggaga agcgatttct tggcatttgg cccatattct
    35341 caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat gaaatcacca aggatgcagt
    35401 caaaaatgct tttaaagaac ctcgtaagat cgatatggac ttggtcgatg cccaacaagc
    35461 tcgtcggatc ttggatcgct tggtagggta ttcgatttcg cctattttgt ggaagaaggt
    35521 caagaagggc ttgtcagcag gtcgcgttca gtccattgcc cttaaactca tcattgaccg
    35581 tgaaaatgaa atcaatgcct tccagccaga agaatactgg acagttgatg ctgtctttaa
    35641 aaagggaacc aaacaatttc atgcttcctt ctatggagta gatggtaaaa agatgaaact
    35701 gaccagcaat aacgaagtca aggaagtctt gtctcgtctg acgagtaaag acttttcagt
    35761 agatcaggtg gataaaaaag agcgcaagcg caatgctcct ttaccctata ccacttcatc
    35821 tatgcagatg gatgctgcca ataaaatcaa tttccgtact cgaaaaacca tgatggttgc
    35881 ccaacagctc tatgaaggaa ttaatattgg ttctggtgtt caaggtttga ttacctatat
    35941 gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat gaggcggcaa gcttcattac
    36001 ggatcgtttt ggtagcaagt attctaagca cggtagcaag gtcaaaaacg catcaggtgc
    36061 tcaggatgcc catgaggcta ttcgtccgtc aagtgtcttt aatacaccag aaagcatcgc
    36121 taagtatctg gacaaggatc agctcaagct atataccctt atctggaatc gttttgtggc
    36181 tagccagatg acagcggccg tttttgatac catggctgtt aaattgtctc aaaatggagt
    36241 tcaatttgct gccaatggta gtcaggttaa gtttgatggt tatcttgcca tttataatga
    36301 ttctgacaag aataagatgt taccggacat ggttgttgga gatgtggtca aacaggtcaa
    36361 tagcaaacca gagcaacatt tcacccaacc gcctgcccgt tattctgaag caacactgat
    36421 taaaacctta gaggaaaatg gggttggacg tccatcaacc tatgcgccaa ccattgaaac
    36481 cattcagaaa cgttattatg ttcgcctggc agccaaacgt tttgaaccga cagagttggg
    36541 agaaattgtc aataagctca tcgttgaata tttcccagat atcgtaaacg tgaccttcac
    36601 agctgaaatg gaaggtaaac tggatgatgt cgaagttgga aaagagcagt ggcgacgggt
    36661 cattgatgcc ttttacaaac cattctctaa agaagttgcc aaggctgaag aagaaatgga
    36721 aaaaatccag attaaggatg aaccagctgg atttgactgt gaagtgtgtg gcagtccaat
    36781 ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt agcaatttcc cagattgccg
    36841 tcatacccaa gcaatcgtga aagagattgg tgttgagtgt ccaagctgtc atcagggaca
    36901 aattattgag cgaaaaacca agcgtaatcg cctcttctat ggttgcaatc gctatccaga
    36961 atgtgaattt acctcttggg acaagcctgt tggtcgtgac tgtccaaaat gtggcaactt
    37021 cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt gtttgtagca aaggcgacta
    37081 cgaggaagaa aagattaaat aagaggaggg gcccgaaagt aaatttcagg ctctttttgg
    37141 ttagaacttg acaaaataca tcattttatg cgaaactaga agaagattat tttaactagg
    37201 agaagttatg cgtcttatct atctgataat tggtttttta tcactggcct tggctattgt
    37261 tggggttgtt ttacccttgt tgcctacaac acctttcctt ttgttgtcta ttgcttgttt
    37321 ctccagaagt tccaagcgat tcgaagactg gctttatcat accaagctct atcaaacata
    37381 tgtagctgat tttcgcgaga ctaagtctat tacgcgtgaa cgaaagaaaa aaatcatcgt
    37441 atccatttac attttgatgg ggatttctat ttattttgcc cctctcttac cagtcaaaat
    37501 cggtctgggt gctttgacca tctttatcac ttattatctc ttcaaggtca ttccagacaa
    37561 agaatagtta aaacagtagt tatttacctt gataaaattg aaagcatatt cataacaata
    37621 tgatataata aatttgaagt aatattcaag gagaatcaaa tgatttacga attttgtgct
    37681 gaaaatgtga ctttacttga aaaagcgatg caggctggag ctcgtcggat tgaactctgt
    37741 gataatctag cagttggtgg gacaacacct agctatggag tgactaaggc agcggttgaa
    37801 ctggcagcta actacgatac aaccatcatg accatgattc ggccacgtgg tggtgacttt
    37861 gtctataatg acctagaaat tgctatcatg ctagaagaca tttgtttgac tgctcaggct
    37921 ggaagtcaag gggttgtatt tggagcttta actgctgata aaaagttgga taagcctaat
    37981 ctggaaaagt taattgctgc atcaaaagga atggaaattg tctttcacat ggcctttgat
    38041 gaactaagtg atgaagatca accggaagct attgactggc tcagtcaagc cggtgtcact
    38101 cgtatcctaa ctcgtgctgg tgtgtctggc gactccttag aaaaacgttt tgttcactat
    38161 cacagaattt tggagtacgc taaaggtaaa attgaaattc taccaggtgg ggggattgac
    38221 cttgacaacc gtcaaacctt tattgaccag gtggtggtaa cacaattgca tggtactaag
    38281 gttgtttttt aaaaaataga aaggaactgc tagccttggg tagcagtttt cacttatgtt
    38341 tgaaattttt aaatcctatc aatttaatca agaaaaggct catgattatg gttttataga
    38401 aaatagcgaa gtctggacat atagttgcca gattttgcaa ggtgactttg tcatgactgt
    38461 gtccatcact gctgataatg tgaactttca agtctttgac caagagactg gtgacctcta
    38521 tcctcacgtt catatggaaa gcatgagggg aagttttgtc ggaaatgtcc gtgaggcttg
    38581 tctggagatt ctttaccaga ttcggaaggc ttgttttgat gtgcaagatt ttatctgtca
    38641 tcagactaag cgtatcatga ctcaagttca ggaaaagtat ggaaaccagt tggagtatct
    38701 gtgggaaaaa tcgcctgata cagctgtatt gcgccatgaa ggcaatcaaa agtggtatgc
    38761 cgtcttgatg aaaatctctt ggaataagct ggaaaagggc agagaaggac aagtggaagc
    38821 agtcaacctc aagcatgacc aagtagctaa tttgctttca caaaagggga tttatccagc
    38881 cttccatatg agcaagcgct actggattag tgtgtccctt gatgatactt tatcagatga
    38941 agaagtactg gaattgatag aaaaaagttg gaacttaacc tctaaaaaat gaaatatttt
    39001 aataattttc atgaactttc aattagctaa atattcttta ctgaagaaat ttttagaaaa
    39061 tataggattt accacactag aggaatatgg tgccatcttc aaatacctga ttgagaatgt
    39121 caagacggat cgtcagatca tctgttcgcc tcactgtcat gatgacctcg gaatggcagt
    39181 ggcaaatagc cttgctgctg tcaagaatgg tgcaggacgt gttgaaggga ctatcaatgg
    39241 tattagggag cgagctgaaa atgctgcttt ggaagaaatt gcagtggctc tcaatattcg
    39301 ccaagattac taccaagtag aaaccagtat tgtcctaaat gagaccatca atacgtcaga
    39361 aatggtttct cgcttctctg gtattccagt tcctaaaaac aaagccgtcg ttggtggcaa
    39421 taccttctcc cacgaatctg gtattcacca agatggagtc cttaaaaatc ctctcactta
    39481 tgagatcatc acacctgaat tggttggtgt taagattccg cttggaaaat tatctggtcg
    39541 ccatgctttt gttgagaaac tgagagaatt ggccctagat tttacagaag aggatatcaa
    39601 accactcttt gctaagttca aggcactggc cgataagaag taagaaatca cagatgcaga
    39661 tcctcgagct ttggtagctg gaaccatggt tgaaaatcca gaaggcttcc actttgatga
    39721 tttacaactt caaactcatg cagataatga cattgaagcg ctcgttagcc tagccaatat
    39781 ggatggtgag aaagtcgaat ttaatgcgac agggcaaggt tccgttgaag caatctttaa
    39841 cgctatcgat aagttcttta accaatctgt tcgtttggtg tcctacacta tcaatgcggt
    39901 aacagatgga atcgatgccc aggatcgggt tttggtcact gttgaaaaca gagatacaga
    39961 aaccatcttt aatgcagcag ggcttgattt tgatgtgttg aaggcttctg ctattgtcta
    40021 tataaacgct aatacctttg ttcaaaaaga gaatgcaggt gagatgggac gcagtgtttc
    40081 ttaccacgat atgcctagtg tgtaaaggag aaggctatgg caaagaaaat agtagctcta
    40141 gtaggagacg gaattggccc agaaatcatg gaggctggtt tagaagttct ggaggctcta
    40201 gctgaaaaaa caggttttga ctatgagatt gacagacgac cgttcggagg tgcagatatt
    40261 gatgcagcag gacctccctt acctgatgaa acccttaagg caagtaggga agcagatgct
    40321 atcctactag cagctatcgg tagtcctcag tatgatggag cagcggttcg ccctgaacaa
    40381 ggcctgatgg ctctccgtaa ggaactcaat ctttacgcta atattcgtcc tgtaaaaatc
    40441 tttgacagtc tcaagtattt gtcaccactc aaaccggaac gaatttctgg tgtagacttt
    40501 gtcgtggtgc gtgaattgac aggcgagatt tactttggag atcatattct tgaagagcgc
    40561 aaagcgcgtg atatcaacga ctatagctat gaggaagtgg agcggattat tcgcaaagcc
    40621 tttgaaattg caagaaatcg cagaaaaatc gttactagta tcgataagca aaatgttcta
    40681 gcgacctcaa aactctggcg gaaagtagct gaggaagtcg cacaggattt cccagatgta
    40741 accttggaac accagctggt agactcagct gctatgctta tgattaccaa tcctgctaag
    40801 tttgatgtta ttgtaacgga gaatcttttt ggagatattt tatctgatga atcaagcgtc
    40861 ttatctggta cacttgaggt tatgccatca gccagtcatt ctgaaaatgg accaagtctc
    40921 tatgaaccta ttcacggttc agcacctgat attgcaggtc aaggaattgc caatcctact
    40981 tccatgattt tatcagttgc catgatgttg agagatagtt tcggacgtta tgaggatgca
    41041 gagcgtatca aacatgctgt tgagacaagt ctggcggcag gaattttaac gagagatata
    41101 ggaggtcagg cttcaacaaa ggaaatgacg gaagctatta ttgcaaggtt atgaagttag
    41161 acgaaaaaat tactctagtc cttttgattt ggaatgtcat cattttcttg atttatggca
    41221 ttgacaaatt caaggcaaga agaagaactt ggcgcatcca agagaaaatc ttactcattt
    41281 tagcccttac ttgtggtggt tttggggcct ggttagctgg aatcactttt caccacaaga
    41341 ctcgaaaatg gtactttaaa acagtttggt ttcttgggat ggtgaccaca ctagtagcct
    41401 tatattttat ttggaggtaa tggatggcag ggtcttcgag ggaacacgct gcttgggttc
    41461 tagcggacta tggttttaag gtcgtgattg caggatcttt cggtgacatt cattacaata
    41521 atgaactcaa taatggcctg ttgccaatcg ttcagcctag agaggttaga gagaaactag
    41581 cccagctaaa accaaccgac caggtaactg tggacttgga acaacaaaaa atcatctcac
    41641 cagttgaaga attcaccttc gagatagata gcgagtggaa acataaactc ctaaatagtt
    41701 tggatgatat cggtattacc ttgcagtatg aagagttgat tgctgcttat gaaaaacaac
    41761 gaccagccta ctggcaggat tagaaaaaat agaaaaggaa atatagtaaa ctgaaataag
    41821 atgtaaacaa atgaattaga gcttaacatc catttccagc aattttttag aaactacagt
    41881 ggactattct ggattcaaca cattataaaa ttatgacaaa acacattcac aagaaggcta
    41941 cgacatttta aaaggtgagg gcggatgtat cgtttgccct actaaagttg gttacattat
    42001 catgaccagc aacaaggcag gacttgagcg taagttcgca gccaaagaac gtaagcgtaa
    42061 caaaccaggt gttgttctct gcggtagcat ggatgaactt cgcgctttag cgcaactcaa
    42121 cccagaaatt gaagcattct actaaaaaca ttggatgaag atattcttct tggttgtatc
    42181 cttccttgga aaccagaagc ctttgaaaaa ctcaaagcat acgggaatgg ccgtgaagaa
    42241 cttatgactg atgtacgtgg tactagctgt tttgttatca agtttggaaa agcaggtgaa
    42301 caattggctg ccaagctttg ggaagaaggt aaaatggtct acgcctcata tgcttcaatg
    42361 acaaaacgat tgaaactcgc tatgagagca aggtgtaatg gtgtctatgg tcgataagga
    42421 cggcaaactc atcccagaac aaggaggagc acgttcaact tcaccagcac cagttgtgat
    42481 ccgtaaaggg cttgacattg ataaaatcat gatgcacctg tcagatactt ttaactcatg
    42541 ggactaccgt caggttgagt attattaaga taaaaaaaga agtctagtgt tatgagatat
    42601 taaagctcct aacactggac tttttgttta gaatttcttt tctttttcta taggatatgg
    42661 tattctatgt agaaaatata tgttaataag taatgccaat atttaaacat cattagtaaa
    42721 aggagttaga ttgatgaata aaagaaaagt tagtttagaa gatttttata aatggtatag
    42781 tctaaataaa gaagagttat taaataaggc aactgttggt gaaaagttta atgataaatt
    42841 aaaagaagag tttctccagg aatggccttt ggataggatt ttaacaatgt caatcgatga
    42901 atatgtaata ggaaagggac agcaaaataa gtctttatgc tacgctcttg agaagggaaa
    42961 atacaaaaat ctatttcttg gaatttctgg tggctcagct tcaaaatttg gtatttattg
    43021 gaataaaaaa acaaacaaat ataaagatca agctaataat gagatttcag agttggatca
    43081 gcgattttca aaattaaaat cagatttgta tgaaattatc aaagaaggta ttcgttttaa
    43141 ctttgaaaat cctatttttg atatgaaaag atcaacaaat gaatttattg gtcgttctgc
    43201 tatggtgaca aaattacttt gtatctatac tgagggagat cctttctttg gtgtaaatat
    43261 taatagtcag aaagaatttt ggaaccactt tgtttctcag acaaatcaag gtggacctta
    43321 tctgcaaaat cataaaataa ttgaactagt gtccaaaact tatcctgagt tggagccatc
    43381 gaaattagga actatgcttt ttgagtattc taagcttttt atggaaaata aggaagacaa
    43441 tagtacaatg gattcatcaa acaattttcg tcatcaatta actcaatctc tattaaagtc
    43501 tccaaacctc atcctccgtg gtgctcctgg cacaggaaag acttatcttg ctaaagaaat
    43561 tgccaaagaa ttaacggatg gcaacgaaga ccaaatcgga tttgtacaat ttcacccatc
    43621 ctatgattat acggattttg tagaaggttt aagaccagta tcaaatgggg atggagctat
    43681 tgagtttagg ctacaggacg gtatttttaa agatttttgt cagaaagcaa aagaaaccca
    43741 attgattgga ggacaagata attttgatga ggcttgggat tcttacttag aatatataaa
    43801 tgttgctgaa gaaaaagaat atataacaaa aacatcttac ttatctatta atagtagaca
    43861 aaatttgtca gtaaattatg atagtggtgt ttcaggatgg tcactaccta gcaaatatgt
    43921 ttacgagttg tataaagata aaaattataa taagcaagaa tactacaaaa gtggtggaaa
    43981 aactgtccta gaaacattga gaaagagatt tggtttgaaa gactatgttt ccccaacaga
    44041 aattgatact gataagaaat tcgtcttcat catcgatgaa atcaatcgtg gggagatttc
    44101 taagattttt ggcgaactct ttttctctat cgaccctggc tatcgtggtg aaaaaggaag
    44161 tgtttctacc caatatgcaa atctacacga aactgatgaa aagttctata tccccgaaaa
    44221 tgtttacatc atcggaacta tgaatgatat tgatcgttca gtggatacct ttgattttgc
    44281 tatgcgtcgt cgttttcgtt ttgttgaagt tactgccgag ggtcaagttg gcatgttgga
    44341 taaagagctg aatatccatg cagaagaagc aaaaattcgt ctaagaaact tgaacgctgc
    44401 tatcgaaaat gttcaggaat taaacagtca ttatcatatt ggaccaagtt attttcttaa
    44461 gttgaaggat gtagattttg actatgaatt actctggtct gattatatta agcctctcct
    44521 agaagactac ttgcgaggtt cttatgatga ggttgaaact ttggaaactt tgaaaaaagc
    44581 atttgaactg acaaataatg atcaaactgg tcaacaagat actggtgata atgatgcgga
    44641 ttactgataa tcaatacaag attattaaag aaaaatttgt tgaagaatat cctaaactaa
    44701 gcaatcttct tttagacaga accttggaaa gtctatccca agatgaacgt attttcattt
    44761 ttccaaatga tttgactcat actcctgatt tggataagga ccaaaagatt tttgaaacag
    44821 tcaatcagaa aatcaagaca gggaacgtga ttggttttct tggatatggt caggaaagat
    44881 taacgatttc ctcacgattt tctgatgaga gtaatgacca ttttttgcat tatctcttaa
    44941 acaaggttct tcatatcaat ctcactagtt tagatgttgc tttgtctcgt gaagagaggc
    45001 tttatcaact tttgatgtat ctcttcccca agtatctaca agctgctatt cgaaaaggtc
    45061 tttataagga ataccagaga ttttctcata acgacagtca tgttaaggga gtgattgatg
    45121 taagaaacca tctcaagaaa aatcttcctt tcacgggaaa tattgcctac acaacgagag
    45181 agttcaccta tgataatccc ctcatgcagt tggtccgtca cactattgaa tacattaaga
    45241 atcagaaaag cattggtcaa ggggtactag ataatctctc aactagtcgt gaaaacgtat
    45301 ctgaaatcgt gcgtgtaacg ccctcttata aactagctga tcgtgctaag attattcggg
    45361 gaaatcaatc taaacctata cgtcatgcat actttcacga gtacagaaac ttacaagaac
    45421 tttgtctgat gatcctaaac caagaaaagc acggtttagg gtatcaagat caaaaaatct
    45481 atggtattct ctttgatgtt gcctggcttt gggaagagta tgtttacacc ttgttgccaa
    45541 aaggttttgt acatcccaga aataaggata agacggatgg aatttcagta ttttctgttg
    45601 ggaaacgaaa agtatatcca gatttttatg acagagaacg aaagattgtt ctagatgcaa
    45661 aatataaaaa actggaattg actgaaaaag gaatcaaccg tgaggactta ttccagctga
    45721 tttcctattc ttatatttta aaagctgaga aggctggact tgtttttccg agcaaggaca
    45781 aggttattga taatgagatg ggaaacctag caggttatgg agctttgttg aagaaatggt
    45841 ctatccaaat tcctcagaag tcttcatctt atagtgcatt ttgtaaaatg atggaaaatt
    45901 cagaagaaaa ttttaaagcg attattgatg aagaagtggg gagaaaataa tttcctctct
    45961 ttcttttatt tttaacacaa gtaaaacccg aacaatatat ttcaattcat aaaataattt
    46021 gacaaaaact gtactttggt ttataataaa ttaaggaaac gtcggaaaag acgtagtgat
    46081 gcgagagcat aggtaggtca ttacaaaaga aacgagacat cgatatgtta aatgaatttc
    46141 caatttttga ttacgaagat attcaattga ttccaaataa atgtgtcatt aaaagccgtg
    46201 cagaagcgga tacaagtgtc actttaggaa atcacacctt taaactacct gttgtgccag
    46261 cgaatatgca gacgattttg gatgaaaatg tagcagagca actggctaaa ggtggttact
    46321 tctacattat gcaccgtttt gatgaggcag gacgcattcc ttttattaaa cgaatgcacg
    46381 atcaagggct cattgcttct atctctgtcg gtgttaagga ctatgagtat gatttcgtta
    46441 gacagctcaa gactgatgct cctgagtata tcactattga tattgctcat ggtcatgcgg
    46501 atagcgtgat ttctatgatt caacacatca agaaagaatt gccagatacc tttgtcattg
    46561 ctggaaatgt gggaacacca gaagctgtgc gtgaattgga aaatgctggt gcggatgcta
    46621 ctaaggtcgg aatcggtcct ggtaaggttt gtatcaccaa ggttaagact ggttttggta
    46681 caggtggttg gcagttggct gctctacgct ggtgtgccaa ggctgcgcgt aaaccgatta
    46741 tcgctgatgg aggaattcgt actcacggtg atattgccaa gtctatccgc ttcggtgcta
    46801 gcatgatcat gattggttcc ctctttgcag gacacattga aagtccaggg aaaacgattg
    46861 aagtcgatgg tgaacagttc aaagaatatt atggttcagc ctcacaatat cagaaaggtg
    46921 cttacaaaaa tgtggaaggc aaacgtatct tgcttcctgc taaaggtcat ctgcaagaca
    46981 ctttaactga gatggaacaa gaccttcaaa gtgctatttc ttatgcaggt ggacgtcagg
    47041 ttgctgacct taaacacgtt gattatgtga tcgtgaaaaa ctcaatctgg aatggggatg
    47101 cttcccacta agacggggtc tttgactaaa aaacttgtta ttagagcaaa tttctgttat
    47161 aataaaacaa gtttccaccc ttagtgtaat ggatatcacg taagattccg gttcttgaga
    47221 tgggggttcg attccctcag ggtggatgta aacatcctaa aaagccttta atagggcttt
    47281 tttctttaca ctgctctaaa tttgccgcta aaactgagaa tctctgtcta aagaattccc
    47341 aaatgctttc cagtcccgtt ttagagtgac tcagggggct ttttttgata taataaaaag
    47401 gactgttatc agttagaaag aggttggtat gaaagaatta caaactgtac taaagaacca
    47461 ttttgcaatc gaatttacag acaaaaattt actggaaact gcctttactc atacgagtta
    47521 tgccaatgag caccgcctct taaaaatttc acacaatgaa cgcttggaat ttttaggaga
    47581 cgctgttcta cagttattga tttcagaata tctgtataaa aaatatccta aaaagcctga
    47641 aggtgaccta tcaaaactcc gtgctatgat tgtccgtgag gagagtttag ctggttttgc
    47701 gcgtgattgc cagtttgacc agtttatcaa gttgggtaaa ggggaagaaa aatcaggtgg
    47761 tcgcaatcgt gacaccattc ttggtgatgc ctttgaagcc tttcttggtg cccttctttt
    47821 ggataaggat gtggccaagg tcaaggaatt tatctatcaa gtcatgattc ctaaggttga
    47881 agcaggcgag tttgagatga ttacagacta taaaacctat ctccaagagt tgcttcaggt
    47941 caatggtgat gtggctattc gttatcaggt gatttctgaa acagggcctg ctcacgataa
    48001 ggtctttgat gtagaagttc ttgttgaagg taagagcatc ggtcaaggcc aaggtcgttc
    48061 taagaaatta gcagagcagg aagctgccaa aaatgccgtt gagaaggggc tggattcatg
    48121 tatttaaagg aaatcgaaat tcaggggttc aagtcttttg ctgataagac caaggtcgtt
    48181 tttgaccaag gtgtgacggc agttgttgga cccaatggat ctggaaagtc caatattaca
    48241 gaaagtctgc gttgggcttt gggggagtct agtgtcaaga gtctccgtgg gggcaagatg
    48301 ccggatgtca tctttgctgg aaccgaaagt cgcaaaccgc tcaattatgc ttctgtagtt
    48361 gtgactctgg ataatcatga cggatttatc aaggatgcag gtcaagaaat cagggtggaa
    48421 cgccatatct atcgtagtgg agatagcgaa tacaagattg acggcaagaa agtccgtctg
    48481 cgtgatattc atgacctctt cttggatact ggattgggac gagattcctt ctctattatt
    48541 tcccaaggga aggttgagga gatttttaat tccaagtctg aggaacgacg agctattttt
    48601 gaagaagctg ctggagtttt aaaatacaag actcgcagaa aagaaaccga gagtaaactg
    48661 cagcaaactc aggataatct ggaccgctta gaggacatta tctacgagtt ggataatcaa
    48721 atcaagcctc ttgagaagca agctgagaat gcccgtaagt ttttagactt ggaaggacaa
    48781 cgtaaggcta tttatttaga cgttctggtt gctcaaatca aggaaaataa ggcagaacta
    48841 gagtcgacag aagaagagct ggctcaggtt caagaactct tgatgagtta ttaccaaaag
    48901 cgtgaaaaat tagaagaaga aaatcaaact cttaaaaagc aacgccaaga tttacaggat
    48961 gaaatggcca aagaccaagg cagtttgatg gacttaacta gtctgattag tgatttagaa
    49021 agaaaattag ccctatcgaa actggagtcc gagcaagtgg ccttgaatca acaggaggca
    49081 caagctcgtt tggctgcttt ggaggataag agaaattcac tcagcaaaga aaagtatgat
    49141 aaagaaagct ctttagctct gttagaggga aatctagtcc aaaataatca aaaactcaat
    49201 cgtttagaag ctgaattgct ggctttctca gacgatcctg atcagatgat tgagctctta
    49261 cgtgaacgct ttgtagctct tttacaagaa gaagcggatg tctcaaacca gttgacccgt
    49321 attgagaatg agttggaaaa tagtcgtcag ctttctcaaa aacaagcaga tcaactagaa
    49381 aagctgaaag agcaattggc tatagctaaa gagaaggcta gtcagcaaaa ggaagagctt
    49441 gaaactgcca aggagcaggt tcagaaatta ttggctgact accaagctat tgccaaggag
    49501 caagaggagc agaaaacttc ctatcaagct caacaaagcc aactctttga ccgtctggat
    49561 aatctcaaaa acaagcaggc cagagctcaa agtttggaaa atatcctgag aaatcatagt
    49621 aacttttatg caggtgttaa gagtgttctc caagaaaaag atcgcctagg tgggattatt
    49681 ggtgcagtca gtgagcatct gacctttgat gtttattatc aaactgccct agagattgcc
    49741 ttaggggcaa gtagccagca tatcatcgta gaagatgaag agtcggcaac caaggctatt
    49801 gacttcctta aacggaacag agccggtcgt gcaacctttc ttcctttaac cactatcaag
    49861 gcgcgtacga tttctagtca gaaccaagat gctatcgctg taagcccagg tttccttggg
    49921 atggcagatg agttggtgac ttttgatact agactggaag ccattttcaa gaacttgcta
    49981 gctacgacgg ctatctttga taccgtagaa catgcgcgtg cagcagctcg ccaagttcgt
    50041 tatcaggttc gtatggtgac attggatggg acagaattac gcacgggtgg ttcttacgca
    50101 ggtggagcca atcgtcagaa caacagtatt ttcatcaagc cagaactgga gcaattacaa
    50161 aaagaaattg ctgaagaaga agcaagtttg cgttcagatg aagtgagttt gaagaacttg
    50221 caagatgagc tagctagatt gacagaaaga ttagaagcca tcaaatctca gggagagcag
    50281 gctcgtattc aggagcaagg cttgtccctc gcctatcagc agaccagtca gcaagttgaa
    50341 gaactggaaa ctctttggaa actccaagaa gaggaaatag atcgtctttc cgagggagat
    50401 tggcaagcgg ataaggaaaa atgccaagag cgtcttgctg caatcgccag tgacaagcaa
    50461 aatctggaag ctgagattga agagattaag tctaacaaaa atgccatcca agaacgctat
    50521 caaaacttgc aggaagagct agcgcaagct cgtttgctta agacagaact gcaagggcaa
    50581 aaacgttatg aagttgctga tattgaacgc ttaggcaagg aattggacaa tcttgatttt
    50641 gaacaagaag aaatccagcg ccttcttcaa gaaaaggttg ataatcttga gaaggttgat
    50701 acagaattgc tcagtcaaca ggcggaagaa tccaaaactc agaaaacaaa tctccaacaa
    50761 ggtttgattc gccagcagtt tgagttggat gatatagaag gtcaactgga tgatattgcc
    50821 agtcatttgg atcaggctcg ccagcagaat gaggagtgga ttcgcaagca aacacgtgct
    50881 gaagccaaga aagaaaaggt cagcgaacgc ttgcgccatc tacaaagtca attaacagac
    50941 cagtaccaga ttagctatac tgaagctcta gaaaaagcgc atgaattgga aaacctcaat
    51001 ctggcagagc aagaagttaa ggatttagag aaggctattc gctcactggg tcctgtcaat
    51061 atagaagcta ttgaccagta cgaagaagtt cacaaccgtc tggactttct aaatagtcag
    51121 cgagatgata ttttgtcagc gaaaaatctg ctccttgaaa ccattacaga gatgaatgat
    51181 gaggttaagg aacgctttaa atcaaccttt gaggccattc gtgagtcctt taaagtgacc
    51241 ttcaagcaga tgtttggcgg aggtcaggca gacttgatat tgactgaggg cgacctttta
    51301 acagctggtg tggagatttc tgttcaacct ccaggtaaga aaatccagtc gcttaacctc
    51361 atgagtggtg gtgaaaaagc tctatcggct cttgccttgc tcttctccat tattcgtgtc
    51421 aagactatcc catttgtcat cttggatgag gtagaggctg cgctggacga agccaatgtt
    51481 aaacgttttg gggattacct caaccgcttt gacaaggaca gccagttcat cgtcgtaacc
    51541 catcgtaagg gaaccatggc agcggctgat tctatctatg gagtgaccat gcaagaatcg
    51601 ggggtctcaa aaattgtttc ggttaagtta aaagatttag aaagtattga aggatgacaa
    51661 ttaaactagt agcaacggat atggacggaa ccttcctaga tgggaatggg cgctttgata
    51721 tggatcgcct caagtctctc ttggcttcct ataaggaaaa agggatttac tttgcggtgg
    51781 cttcgggtcg gggatttctg tctctggaaa aattatttgc tggtgttcgt gatgacatta
    51841 ttttcatcgc ggaaaatggc agtttggtag agtatcaagg tcaggacttg tatgaagcga
    51901 ctatgtctcg tgacttttat ctggcaactt ttgaaaagct gaaaacgtca ccttatgtag
    51961 atatcaataa actgctcttg acgggtaaga agggttcata tgttctagat acggttgatg
    52021 agacctattt gaaagtgagt cagcattata atgaaaatat ccaaaaagta gcgagtttgg
    52081 aagatatcac agatgacatt ttcaaattta caaccaactt cacagaagaa acgctagaag
    52141 ctggtgaagc ttgggtcaat gataatgtcc ctggtgtcaa ggctatgaca actggctttg
    52201 aatctattga tattgttctg gactatgtcg ataagggtgt agctattgtt gaattagcta
    52261 aaaaacttgg tatcacaatg gatcaggtca tggcttttgg agacaatctt aatgacttac
    52321 atatgatgca ggttgtggga catcctgtag ctcctgaaaa tgcacgacct gaaattttag
    52381 aattagcaaa gactgtgatt ggtcaccata aggaacggtc ggttatagct tatatggagg
    52441 gcttataatg gcagatataa aattgattgc attggacttg gacgggacct tgctgactac
    52501 tgataaaagg ctgacggatc gtaccaagga aaccttgaaa gctgcgcgtg atcgtggtat
    52561 caaggtcgta ttgacaactg gtcgtccctt aaaagctatg gatttctttc tccatgagct
    52621 agggactgac ggtcaggaag atgagtatac cattactttt aatggtggtt tggttcagaa
    52681 aaatacaggt gaaatccttg ataagacagt cttttcatat gatgatgtgg cacgcttgta
    52741 tgagaaaaca gagaaattat cactgccact tgatgccatc tcagaaggaa cagtttatca
    52801 aatccaatcg gaccaagaaa gtctttatgc caaattcaat ccagctttga cttttgttcc
    52861 agttgacttt gaagacctgt ctagtcaaat gacctacaac aaatgcgtga ctgcctttgc
    52921 tcaagaaccc ttggatgcag ccattcagaa gatttctcca gaattgtttg accaatatga
    52981 aatctttaaa tcacgtgaaa tgttgctaga gtggtcacca aagaatgttc ataaagcaac
    53041 aggtttggca aaactaatca gccatctcgg aatcaaccaa agccaagtta tggcctgtgg
    53101 tgacgaggcc aatgaccttt ctatgattga atgggcaggt cttggtgttg ctatgcaaaa
    53161 cgctgttcct gaagtaaagg cagccgcaaa tgtagtaatg ccgatgacca acgatgagga
    53221 agctgtcgcc tgggctatcg aagaatatgt gctaaaggag aactaagata tgggattgtt
    53281 tgaccgtcta ttcggaaaaa aagaagaacc taaaatcgaa gaagttgtaa aagaagctct
    53341 ggaaaatctt gatttgtctg aagatgttga tcctaccttc acagaagttg aggaagttcc
    53401 tcaggaagaa gcagaggttg aaattgttga acaagctgtg ttccaagagg aggaaatcca
    53461 agacacagtt gaagaaagtc aggattcaga gccagctgta ggggtttctc aagaagaaat
    53521 agaagaatta ccaaactcag aagaattacc agaggaagag aagcttgagc acgaaggaac
    53581 tgtagaagaa aataattctg aagttcttga accagaaagg cctcaaacag aagaaactgt
    53641 tcaggaaaaa tatgaccgca gtctcaagaa gactcgtacg ggcttcggtg ctcgcttgaa
    53701 cgccttcttt gctaacttcc gctctgttga cgaagaattt ttcgaggaac tggaagaact
    53761 gctgattatg agtgatgttg gtgtccaagt cgcttctaac ttaacggagg aactacgtta
    53821 cgaagccaag cttgaaaatg ccaagaaacc tgatgcactt cgtcgtgtca tcattgagaa
    53881 attggttgag ctttatgaaa aggatggtag ctacgatgaa agcatccact tccaagataa
    53941 cttgacagtt atgctctttg ttggtgtgaa tggtgttggg aaaacaactt ctattggaaa
    54001 actagcccac cgctacaaac gaactggtaa gaaggtcatg ctggttgcag cagatacctt
    54061 ccgtgcgggt gcagtagctc agctagctga atggggccga cgagtagatg ttccagtagt
    54121 aactggacct gaaaaagctg atccagccag cgtggtcttt gatggtatgg aacgtgccgt
    54181 ggctgaaggt atcgatattc tcatgattga tactgctggt cgtctgcaaa ataaggataa
    54241 ccttatggct gagttggaaa agattggtcg tattatcaaa cgtgttgtgc cagaagcacc
    54301 acatgaaacc ttcttggcac ttgatgcatc aacaggtcaa aatgccctag tacaggccaa
    54361 agaattttcg aaaatcacac ctttaacggg aattgttttg actaagattg atggaactgc
    54421 tcgaggaggt gtggttctag ccattcgtga agaactcaat attcctgtaa aattgattgg
    54481 ttttggtgaa aaaatcgatg atattggaga gtttaactca gaaaacttta tgaaaggtct
    54541 cttggaaggc ttaatctaat cagaagcaaa aatcctgcaa ggtacaaact tgcaggattt
    54601 ttttattcga aacgaccatc ttgacgatag gcgatatctg gttgccaagt ccatttggca
    54661 tcgaattttt caagtaggtc aaagctggct tgaggtccca tgcttccagc tttatagtca
    54721 tgaagtgggg caccattttc ggcccagagc tcttcaatac ggtcaatcaa cttccatgac
    54781 gcaccaacct catcccagtg gctaaagtta gttgagttgt tatttaggac atcataaatc
    54841 aatttctcgt atggttctgg agaagcacca gttgcagtcg cgtctgtacg ataatcaagt
    54901 gagttaggag ccaagttaaa ttcttctcct acttgcttcc catttaggct aagagagaag
    54961 ccttctgttg gttgaatata gatggtcaaa atatttggag caagtggttc tccaaagata
    55021 gaatccattt gtttaaagac gatgttgaca tgagttcctt tttcagtcag tcgtttacct
    55081 gtacggaaaa agaaaggaac accacggaat cgatcgctgt ctacaaagaa ggcaccagat
    55141 gtaaaggttt cagttgttga ttctggattc acatttggtt cgctacgata agagatgtat
    55201 ttcatgccat caatcttacc agagcggtat tgtccacgga taaagtgttc tttgagttct
    55261 tcatcagttg gatgatagag gtttttaaag accttaatct tttcagcacg aatctcgtct
    55321 tttgtgaagc ttgctggttt gtccatggcg aggagcgaaa gaagttgtag agtgtggttt
    55381 tggaccatgt cacggagggc accggattgg tcatagtagc caccacgttc ttctacaccc
    55441 aagcgctccg caaaggtaat ttgaacattg tcgataaaat ccttgttcca aacgttttca
    55501 aaaatcaagt ttgcaaagcg aactgcaaag atgctttgga tcatttcctt gccaagataa
    55561 tggtcgatac ggaaaatttg ttcttcgtca aatgttgcta ggagttcgtc attcaacttg
    55621 cttgcagttg cgtaatctgt accaaatggt ttttcaacga tcaagcgctc aaaacctttg
    55681 ccatcgacaa tgttttcaga tttgaggtgt ttggcaatgg ttccaaagaa ctgaggtgcc
    55741 atagacaaga agaagagctt attgtgttca gcttggtatt tttcattaag ctcagcttgt
    55801 aattgacgca aagcaatata atgttccgaa tcattgacat catggctttg atagtagaag
    55861 tggctagcaa attcttgggc ttgctcggta ctatctgcca aatcaaggat ggactcgaca
    55921 actacagatt caaaatattc cttactccaa ggtctacggg cagttccaat aacggcaaag
    55981 tgcttggaaa gattgccgga tttatatagt ctaaaaaggg aagggtagag cttgcgttta
    56041 gccaggtctc cactcgcacc gaaaattgta acaataacct tagatgacat ctagctacct
    56101 aatttctatt tttattccta gtcttgctag gtatgaggaa acctcatttc ataaacttaa
    56161 ttctaacata attttccgaa aaatcaaaaa ttccaatacg aggtagaaaa actgcaagga
    56221 aatccttaca gtttgagttt agacgtttaa gaccttatct aagaactctt tcagacgagg
    56281 gtgttgtggg ttatcaaaga tttggtcagg tgttccgtct tcaaggaact cgccatctgc
    56341 agtaaagata acgcggttgg caacctgacg agcaaatccc atctcatggg ttacgataat
    56401 catggtcatg ccttgctcag ccaattcctt cataacgtta agtacgtctc caaccatctc
    56461 agggtcaagg gcagaagttg gttcatcgaa gagcatgatg tctggattca ttgctaggcc
    56521 acgagcgatg gccacacgtt gtttttgacc acctgatagg ctatctggat tggcattagc
    56581 tttatctgct agtccaacct tttcaagcaa ctccattccc aattcctcag cttcttcctt
    56641 agtcatcaac ttgtgctcaa taggagcaaa ggtgatgttg tccaatacag acatatgagg
    56701 gaagaggttg aagtgttgga ataccatgcc gatattttca cggacgtggt caacattggt
    56761 tgttttttca gttaaatcat agccgttcac agtgatgtga ccgctagtga cttcttctaa
    56821 aagattgagg ctacggagga aagttgactt accagaacct gaaggaccga tgatacaaac
    56881 aacatctcct tcatagaact tagtcgtaat tccttttagg acttcatttt ttccatagtg
    56941 cttgtgtaaa tcatttacat caatttttaa ttttgccatt aacgaatcct cttttctaag
    57001 cgtttcgcta gtctagtcaa aagtgtgata attacaagat agaagatagc aaggattgca
    57061 tacatcttga aactttggta gttacgagca atgataatct taccagtttg gaagagttca
    57121 accaaaccga tagcagatac gatagttgta tctttaagag cgataacgaa ttggttgaca
    57181 aagtttggca acatcaattt agttgcttgt ggcaagataa tcttacgcat ggtttttcca
    57241 taagagatac ccaagcttcg gctggcttcc atttggccaa ctggaacggc ctgaatacca
    57301 ccacgaacga tttcagcgat ataagcagcc gcattgagtg agagggcaat tgttccagct
    57361 acaaagtcgt taattgggct ttgttggcct gtgatagact cgatgaagtt tggaattccc
    57421 cagaagatga aggctgcaag aatcatcaat ggaataccac gaataacgtc aacgaaaatc
    57481 tcagagatga cgcgaagaga tttgtatggg ctaacgctaa acataccgaa gataattccg
    57541 atgacaatgg caatagcaaa tgagataaga gctagagcaa gagtgatacc aagaccgcta
    57601 aggagttgtt tgtaattgtt ttgaagcaag ccccagagcg ttgtttcgtc aacagtactt
    57661 gttgaagcag ttgaagattc gctagctagg tatttgtcaa gaatcttttg gaattcaccg
    57721 tttgctttaa ggtttgcaag tccgttgttg aacatttcaa tcagttctgg atttgctcct
    57781 tttttaacgg caaaggctgt ttcaccgatt ggagttccag agattggagt tttcaatttt
    57841 tgaccttggc tgatagaata tttgagaaca ggttcatcat ccataacggc atcaatggca
    57901 ccagtattta aactgtcata cattgaagaa ccatcagcaa aggttttgat tttgtagccg
    57961 tatttgcttt gattttctgt taggaaggtt tgagaagcag ttccgttttt aacaccgact
    58021 gtctttcctt ttagatcttc ataagaagca atattgcttg attctttgac accaagaatg
    58081 gtattagcag tgtagtatga ttctgagaag tcaaaagttg ccttacgagc atctgtgaca
    58141 gacataccag cgatgatacc atcggcttga ccagcttgga cagcactgat agcagcatca
    58201 aaaccagggt tggtgatttc aatttcaaaa ccttggtctt tagcgattgc cttaatcaat
    58261 tccatatcaa taccagtgta ttggttgctt gaattttgga aaacaaaagg tgcaaaagaa
    58321 gaatcgctgg caatgatata tttagcctta acaggaatgg cttttaatcc tgcgagagta
    58381 gttgtagttg gcactgctga agatgatgaa gcagtccatt tcttgataat tttatcaaga
    58441 ctaccatctt ttttcatttc agacaaggct tggttaaatt cagtaaccag gtgctcgtat
    58501 ttacttcctt ttttcacacc gaaagcaaaa cttcctacag cttcaccatc catttcaata
    58561 tggaggtctt gaccttggtt aatggcatat tcgataacag gtttgtcatc catcatggca
    58621 tcgatggcac cagcactcaa gctgttgttc attaaatcac cagtgtcaaa tgttttaata
    58681 gtaaagccgt atttatcttt gattgtttca aggaaacgtt gagcggcagt tccgttttta
    58741 acaccaacgg ttttgccagt taattggtcg tacttgctaa ttttgtgtga ctttgtagta
    58801 gcaatgacaa cttttgtatc atagtaagta tcagacatgg tgaagacttt ttcacgttct
    58861 ttagtctttg tcatccctgc catgatagcg tcggcttgcc cagcttgaac cgcattgact
    58921 gctgcgtcaa atccaggata ggacatctga atgttccagc ctttaatctc agcgactttg
    58981 ttaataatgt caacatcaat tcctttataa gtttgatctg aatctttaaa ctcaaaaggt
    59041 gcataggcgg tatcagaaac aatcttaatc gtttctgctt tggcaatacc taatgagaaa
    59101 attgggaata aaattagcaa aaatgctaga aattttttct tcatttttag tctccttttc
    59161 cgaatattct ctcattatat cagaaaaagt aaaaaaaggc aaatttttat atttttcgtg
    59221 tcagaaaata taacatcgat tttttccttt ccttgaattg ctattaaaaa gtgctataat
    59281 aatagtatat atagaagaaa agaggacggg gatatgaaga acaaaagaat atttaaagac
    59341 ttccaagctt caaaaatgag tttaaacatt tacacaagcc ccttgttagc ctttgttttt
    59401 gtcttcatag gagagtttgt ggcttttact ttgtatggta ttggcttgtt agctctcatc
    59461 ggacttgcta gaaattttgg agaggctggt caaaatcttg caagctactt gcagaccttg
    59521 catcagagct tgacggataa aacaagtgac tttcgtttaa ttttaggatt actggccttt
    59581 ggttttattc ttaacactgt gttcagatgg acaagaaaag ttgagaaaag acctattcga
    59641 accttgggat tttatagaga gaatttcctc agcaatcttc tgaaaggatt tagtctaggt
    59701 ctggcacttt ttcttctgac cttgttaggt ttggtggtct taggtcaata tcgtttggaa
    59761 tccattcact tgaatcctta ttctcttgcc tttgtcgtct ttactatccc attttggatt
    59821 ttacagggga cagcagaaga agtggtggcc cgtgcttggc tacttcctca attggcctca
    59881 agaaccaatc taaaactagc tattcttata tctagcctgt tctttaccct gcttcatatg
    59941 ggcaattctg gcctcacccc tctatctcta gtaaatctct ttttattcgg agttgccatg
    60001 gctctttacc ttctcaaaac tgatacagtt tggggtgttg caggtattca tggtgcttgg
    60061 aattttgctc agggtaatct ctttgggatt ttagttagtg gtcaaccgtc aggaacgtct
    60121 ctgatgacct ttttaccaca aggcaatcaa gattggctat caggtggttc ttttggcata
    60181 gaaggttcca ttatgacaag tctggtatta ctactgctga ttgtctatct tgccaataaa
    60241 ttaaagaaag aaaatgaaag gatgtgactt cggtccgtcc ttttcttcgt gaaaatacta
    60301 taagtatgct aaaataggaa tagcacatgg agagaggatt cttatgatca atcacattac
    60361 agataatcaa tttaaactag tatcaaaata tcaaccatca ggagatcaac cccaagctat
    60421 cgagcagttg gtggataaca ttgagggggg agaaaaagct cagattctga tgggggcgac
    60481 tggaacaggg aagacctata ctatgagtca ggtcatttct aaagtcaata aaccaactct
    60541 ggttattgcc cacaataaaa ctctggctgg tcagctctat ggggagttta aggaattttt
    60601 ccctgaaaat gcagttgagt atttcgtatc ctactatgat tattaccagc cagaggccta
    60661 tgtcccttct agcgatacct atattgagaa ggatagttct gtcaatgacg agattgacaa
    60721 gcttcgccac tcagctacct cagccctttt ggagcgtaat gatgttattg tcgtggcctc
    60781 agtctcttgt atctatggtt tgggttcgcc caaggaatac gctgatagtg tcgttagtct
    60841 ccgtcctggt ctagagattt ctcgtgataa actcttgaat gacttggtcg atattcagtt
    60901 tgaacgtaat gatattgatt tccaacgcgg aagatttcgc gttcgtgggg atgtggtaga
    60961 gattttccca gcttcccgag atgaacatgc ctttcgagta gaattttttg gagacgaaat
    61021 tgaccgtatc cgtgaggttg aggctctgac aggtcaggta ttgggagagg tggatcattt
    61081 ggcgattttc ccagctactc actttgtgac caatgacgac catatggaag ttgccgttgc
    61141 caagattcaa gcagaattag aagagcagtt agctgtcttt gagaaggaag gtaaattgct
    61201 tgaagcccag cgcttgaaac aacggacaga gtacgatatc gaaatgctgc gcgagatggg
    61261 ctatacaaat ggggttgaaa attattctcg ccacatggat ggacggagcg aaggagagcc
    61321 tccttatacg cttctcgact ttttcccaga tgattttttg attatgattg acgagagtca
    61381 tatgactatg ggacaaataa aaggcatgta caatggagac cgttcgcgta aggaaatgct
    61441 agtaaattat ggtttccgtt taccgtctgc cttggacaat cgtcctctcc gtcgtgagga
    61501 gtttgaaagc catgtgcatc agattgttta cgtatcagcg acacctggtg actatgaaaa
    61561 tgaacagact gagacggtga ttgagcaaat cattcgaccg acagggcttt tggatcctga
    61621 ggtggaagtc cgtccgacta tgggacagat tgatgatctc ttaggtgaaa tcaatgcccg
    61681 tgttgaaaag aatgaacgaa cctttatcac caccttgacc aagaaaatgg cagaggactt
    61741 gaccgactat ttcaaggaaa tgggcatcaa ggtcaagtac atgcactcgg atatcaagac
    61801 cttggaacgg acggagatta tccgtgatct ccgcttgggt gtctttgatg tcttggttgg
    61861 aatcaatctc cttcgtgaag ggattgacgt tcctgaggtt agcttggttg ccattctcga
    61921 tgctgacaag gaaggtttcc ttcgtaacga gcgtggactc atccagacca ttgggcgtgc
    61981 cgcccgtaac agtgaaggtc atgtcatcat gtatgcagat acggttaccc agtctatgca
    62041 acgtgctatc gatgaaactg cccgccgtcg gaaaattcag atggcctata atgaagagca
    62101 tggtatcgta ccacagacaa tcaagaaaga aatccgtgac ctgattgccg tgaccaaggc
    62161 agttgctaag gaagaggaca aggaagtcga catcaatagc ctcaacaaac aagagcgcaa
    62221 agaactcgtc aagaaactag aaaaacaaat gcaagaagcc gtcgaagtgc ttgactttga
    62281 actggcggct cagatccgtg atatgatgtt ggaagtcaag gccttggatt aggggaatta
    62341 gtatgattta tttaagaaag ttaaagaaag aagatttgat gtctttatgg gaaatggctt
    62401 attcacagct taatccagtt tggaaacagt atgatgctcc ctattatgat gattatcagt
    62461 atttttcaaa ttttaaagaa ttcgaactac aaaaatcaga atccatttta agcaactcaa
    62521 atcgccttgg tatttttgtt gatgataaac tagttgggac tgtttcgcgt tattgggtat
    62581 gtaaagaaac aagatggatg gaattgggaa ttggtattta tgataaaaaa ttctggaaca
    62641 ctggtattgg gaaagttgct atgttgcagt ggatagatag gacgtttcag gattacttgg
    62701 agttggagca tctggttttg acaacttggt caggaaatct tggtatgatg aaacttgctg
    62761 aaaaattaag aatgaaaaaa gaagctcata ttccaaaagt tcgttattat caaggtaaat
    62821 attttgatag tattaaatat ggtattttga gagaagactg ggagaaaata aatgacggtt
    62881 attatcaaat caatggaaac tcctgaagag atagaaggca aatccctcgt tcactggcaa
    62941 acgtggagag agtcttatga tgaccttttg cctgcggaat ttcaggatac aatgacatta
    63001 gaaagatgtc gattctttag tcaaaagtat ccagaaaata cattgattgc gatggatggc
    63061 gtgaaggtgg ttggttttat cagttatggc aattttcgtg atgagactat tcaagctggt
    63121 gaaattattg ctttatatgt tttaaaagac tattatggaa aaggtatcgc acaaaagtta
    63181 atgaaagcag ctttgactgc tcttaatcat ttttctgaaa ttttcttatg ggtattgaaa
    63241 gataacaagc gcgccattgc tttctatcaa aaaatgggtt ttacttttga tggacaagaa
    63301 aaaatacttg aacttggaaa gcctataaag gaaaaacgaa tggtgttcta ttctaaataa
    63361 ttttaaaaaa gtaaaagata atatctctgg tagtaagtct gaaaatttaa taaattagaa
    63421 agcgagtaaa tttatgtccc gttcccaatt aacaatttta acaaatatct gtctgattga
    63481 agacctcgaa actcagcgcg tggtgatgca gtatcgcgcc cctgaaaaca atcgctggtc
    63541 tggttatgcc tttcctggag gtcatgtaga aaatgatgag gcttttgcgg agtctgtcat
    63601 tcgtgaaatc tacgaagaaa cagggttgac tatccaaaat cctcaacttg tcggcattaa
    63661 aaattggcca ctagatacag gtgggcgcta tattgtcatt tgttataagg cgactgagtt
    63721 ctctggtacc cttcaatctt cagaagaggg agaagtttct tgggtgcaaa aagaccagat
    63781 tccaaactta aatctggcct atgatatgct accattgatg gaaatgatgg aagctcccga
    63841 caagtcagag tttttctatc ctcgccgtac agaagacgat tgggaaaaga aaatcttcta
    63901 gtcttttact aaataaccta gctgatccaa ggcctcctcg atatagtgga ggtcttgttg
    63961 tgtttcggct tcgactaggt ggtaatggat gccatctgtc aattcagata gaggtctaaa
    64021 gtcagaatgc tcgacttgtt ctagaaaatg ttgcacatct ctgcgacaag acagtttaag
    64081 caaggtttca atttctccat aaacgggatg gtcaatcaag gtattctgga catgcccacc
    64141 attatcgaca atagcaagga gttcctgacc gatttcttca acttcatgtt tcactttgaa
    64201 aagtttgtga acataaggat tggtatcaat ttgcttatag atgtagccac gattggtgga
    64261 taggatagga gcgccatcgg ctctcaaaat tgcaatgtcc tgcacaatga cctgacgtgt
    64321 gacatgaaaa tgttccgcca aactttggcc attaagggct ttaggagctt ctttcaagag
    64381 ttggagcagg gcttgtttgc gatcttttgt catagctttt ccttttaacg gcgttttcga
    64441 agcactttat agacagctag tgctaatgta tagtctacca tactatggat aattgtgcca
    64501 aatccaacta gtacaaatag aacatagaac atattttcta cattggtacc cgaagttgcg
    64561 taaaaaagga cacaggctaa tacttcagca agggcatgaa caacagccaa aacaaaattg
    64621 aaaacccagg aagattttgg tttatctagg gtatcgggga atttttgtag gtaaagagct
    64681 cctaaagcac caaaagatat atgggaaaaa gcccgaaaaa cgataaccat gggatagcca
    64741 gccatcaaaa atccaaaact agaggctagg atgacaaaaa ctgccatcaa gggcgacaag
    64801 aacatggcta taaaaatagc gatgtggctc cccaaagtat aggaagcagg tggaatgaca
    64861 atcttgaaag gcataacaat tggaatcaaa atcgcaatag ccgttaaaag ggctgtcatt
    64921 gtcataaatt gtgtcttttt ccgtgtattc acaagaatct cctttttaac tgcatataca
    64981 ctagtatggt acaataaacc agacaataaa gcaagaattt acttgggttt atagatcatt
    65041 ttttagttaa aagttatagt agatggaaac tagaatagtc cacctctact tctaaaacat
    65101 tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttatttc gttttactat
    65161 agtaaagatt tcattaaaaa gaaactgtat agagcaaaat ctccaccttc aggtttgaaa
    65221 gcggagattg tttttatttt ttcaaggttt gtagtcgtgg aacaatggct agggttagaa
    65281 ttgcagaaat gaccaattca gcaattgaat ttgttgagat aacggttgcc agaagaagtt
    65341 ggatatttcc attataaaca tttccaaata ggaagaagat tcctccaagg acaaagatag
    65401 tatttgtcaa ggaaccgagg gctccagcta aaatcagacc agtcttgttt ttcatcagtt
    65461 tatagactaa gtaaggagtt aaaccaatca aaatacgtgg gacgatggca atgatagctg
    65521 agtagatgtt tccgtttggt acgaagggag agaagaggta gcttgtcggt agaatcgtaa
    65581 tcgtgttaac cgtcaagcta agtaatccca tcaaaaatcc aagtgtaacc ccaactcgtg
    65641 gaccataaat aatgctggca ataatgacag gaatatgaac aatggtcggt ttgattggaa
    65701 atggaaaaag gttaaagata agtgagctca gaaagtgtat cacgagcatg gtagcaaaaa
    65761 agatagcaat gggtgcaata ttagagcgtt ttttcatcga tagtttcctt tattctttct
    65821 aaaataattg tgaggtcagc taaagctcct cgtccgtggt ctccacaagc tagtagggat
    65881 tccttaggag caatcagctg atagccgtag gtttctaatg ttttcagatt attctgagtt
    65941 actggatggt catacatttt tgtattcata gcaggagcta ttagtttggg aatatgactt
    66001 ggtagggcta gagctgtact ggttaccatg ttgtccgcaa atccgtgagc tagttttgca
    66061 atagtgttag cagttgcagg taccacgata aataaatctg ctttttttcc aagttcgata
    66121 tgattgacct gatcaggata gggttccttc atgacatcca agtggacagg attctgtgag
    66181 agtacctgta gtgtcaaagg ttggataaac tctgtagcag cctgagtcat taagacagtg
    66241 acttgatggc cttgtttttt tagagaactg actaaatctg ccgacttata agaggcgatt
    66301 gaacccgtta cagccaagag aatgtttgcc atagttttcc tttctaagaa tgataggctt
    66361 gaattttttc aaggaggagt tctgcaattt cttctttagt ctggactgtt tgaagctgat
    66421 ttttctcaac aaatatagct cggtgctgat ctgctgaaat ttgagtcagg tcattcgcga
    66481 tgattaaatc tgcttgattc ttgataagac tttttcgtgc aatgtcaacc agatgatctt
    66541 cggtaacatc aaccagcagt ttgaaaccaa tcagatgaat agtgggattc cattccttga
    66601 ctagggatat gattttgggt gtctttttaa ggaacaaaac ctgaacctca tcagttgaag
    66661 aaatcttggc ctgatgattc tgcttgctta aaaattcttt tagattggag ctagcctgaa
    66721 cttcctcaag ccctgtcata taaacaggag tgtagtcaga aacagccatt gagtggatca
    66781 agacctgata atcctgaaca cgttcttgca tttctattag aaggtccttg gtattggtaa
    66841 tttctcgaat acttaggtta ggatgaggct ctggcttcag agctcgtttt gtcgtaatta
    66901 aacaaacttc atgccctgca gaaagcaaag tctctgtgat aattttcccc aagtgacctg
    66961 tagaatggtt agtgatagag cggacgctat cgatagcttc actggtaccg cccgatgtaa
    67021 ctaaaatttt catagcacta ttgtacacaa aaataagcgg taagtaaaat gaaagtgata
    67081 aatttttggt aaaaacgaag atttactata gtttcaaact ataaagccat ataaaattta
    67141 agaaaggact ctaaaatcct taaaaacaga gatatttacg aacgtttaga gagttttagg
    67201 atgttaaaaa tcttgttttg tgatataatg aattatcata taagaggtta ggggaatttt
    67261 gaatgaaaac agatattgaa atcgcacaga gtattgagtt gaagccaatt gttgatgttg
    67321 tagagaaact tggtatttct tatgacgatt tggagttgta cggaaagtac aaggctaaac
    67381 tcagctttga taaaattcgt gcagttgaga gcaatccagt cggtaaattg atcttggtta
    67441 ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac gcttaccatt ggtcttgcgg
    67501 atgccttgaa caagattggc aagaaaacca tgattgctat ccgcgaaccg tctcttggtc
    67561 cagtcatggg gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt ctgccaatgg
    67621 aagacatcaa cctccacttt actggagata tgcatgctat tacaactgcc aacaatgccc
    67681 tttctgcctt gattgacaac cacttgcacc aagggaatga gctggaaatt gatcaacgtc
    67741 gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc gctccgccat gtgactgttg
    67801 gtcttggtgg tcctctaaac ggtattccac gtgaggatgg ttttgatatt acagttgctt
    67861 cagaaatcat ggcaattctt tgcttggcaa cggacatcga ggacttgaaa cgtcgtttgg
    67921 cgaatatcgt tattggttat cgctatgacc gtacgcctgt ttctgtaggt gatttgcagg
    67981 ttgagggtgc cttggctttg attttgaagg atgctattaa gccaaacttg gttcagacaa
    68041 tttacggtac acctgccttt gtacacggtg gtccatttgc caatatcgct catggctgta
    68101 actctgtttt ggcgacgaca acagcccttc acttggctga ttacactgtt actgaagctg
    68161 gttttggtgc ggaccttggt gctgagaaat tccttgatat caagacacca aacttgccaa
    68221 catctccaga tgcagttgtc attgtcgcaa cccttcgtgc ccttaagatg aatggtggtg
    68281 tggctaaaga cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt tttgctaact
    68341 tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc agcagttgta gctattaacg
    68401 aatttgtatc tgatacagaa gctgaaattg cagtcttaaa agaactctgt gcctcaatcg
    68461 atgtaccagt tgaattggct agtgtctggg ctgatggagc agaaggtgga gtagcacttg
    68521 ccgaaacagt tgttaagaca attgctgaaa atccagctaa ctataaacgt ttgtacgata
    68581 atgccctttc tgtccaagaa aagattgaaa aaattgtcac tgaaatctat cgtggtagca
    68641 aagtgaactt tgagaagaaa gctcaaacac aaattgctca aatcgttcaa aacggttggg
    68701 acaaattgcc aatctgtatg gctaaaactc aatacagttt ctcagacaat ccaaatgcgc
    68761 ttggagcacc agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa ttaggtgcag
    68821 gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc aggtcttcca aaacgtccag
    68881 cagctctcaa catggatgtt gaaagcgatg gaactgtact aggcttgttc tagtggattg
    68941 aaattgactt taagtgagtt tggaaataat atccaagctc attttttctc agactgcaag
    69001 cagttcatca ataagattta tactcttcga aaatctcttc aaactacgtc agcttcacct
    69061 tgccgtaggt atatg
//
LOCUS       DAVMMR010000004        51552 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMMR010000004 DAVMMR010000000
VERSION     DAVMMR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.004"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            671..1948
                     /gene="ltrA"
                     /locus_tag="VYS74_000226"
     CDS             671..1948
                     /gene="ltrA"
                     /locus_tag="VYS74_000226"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV3788320.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            2404..3582
                     /locus_tag="VYS74_000227"
     CDS             2404..3582
                     /locus_tag="VYS74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3788321.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLEEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3896..4027
                     /locus_tag="VYS74_000228"
     CDS             3896..4027
                     /locus_tag="VYS74_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV3788322.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4048..5598
                     /gene="dltA"
                     /locus_tag="VYS74_000229"
     CDS             4048..5598
                     /gene="dltA"
                     /locus_tag="VYS74_000229"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV3788323.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5595..6839
                     /gene="dltB"
                     /locus_tag="VYS74_000230"
     CDS             5595..6839
                     /gene="dltB"
                     /locus_tag="VYS74_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV3788324.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVIT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6853..7092
                     /gene="dltC"
                     /locus_tag="VYS74_000231"
     CDS             6853..7092
                     /gene="dltC"
                     /locus_tag="VYS74_000231"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV3788325.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7085..8353
                     /gene="dltD"
                     /locus_tag="VYS74_000232"
     CDS             7085..8353
                     /gene="dltD"
                     /locus_tag="VYS74_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV3788326.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8508..8948
                     /gene="adcR"
                     /locus_tag="VYS74_000233"
     CDS             8508..8948
                     /gene="adcR"
                     /locus_tag="VYS74_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV3788327.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8948..9652
                     /locus_tag="VYS74_000234"
     CDS             8948..9652
                     /locus_tag="VYS74_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3788328.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9645..10451
                     /locus_tag="VYS74_000235"
     CDS             9645..10451
                     /locus_tag="VYS74_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3788329.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10461..11966
                     /gene="adcA"
                     /locus_tag="VYS74_000236"
     CDS             10461..11966
                     /gene="adcA"
                     /locus_tag="VYS74_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV3788330.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13136..13909)
                     /locus_tag="VYS74_000237"
     CDS             complement(13136..13909)
                     /locus_tag="VYS74_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3788331.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            14085..15488
                     /locus_tag="VYS74_000238"
     CDS             14085..15488
                     /locus_tag="VYS74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV3788332.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            15603..16241
                     /locus_tag="VYS74_000239"
     CDS             15603..16241
                     /locus_tag="VYS74_000239"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV3788333.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            16253..16693
                     /locus_tag="VYS74_000240"
     CDS             16253..16693
                     /locus_tag="VYS74_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV3788334.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            16686..17117
                     /locus_tag="VYS74_000241"
     CDS             16686..17117
                     /locus_tag="VYS74_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3788335.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            17108..17578
                     /locus_tag="VYS74_000242"
     CDS             17108..17578
                     /locus_tag="VYS74_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3788336.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            17603..18376
                     /locus_tag="VYS74_000243"
     CDS             17603..18376
                     /locus_tag="VYS74_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3788337.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            18373..19173
                     /locus_tag="VYS74_000244"
     CDS             18373..19173
                     /locus_tag="VYS74_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3788338.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            19232..21526
                     /locus_tag="VYS74_000245"
     CDS             19232..21526
                     /locus_tag="VYS74_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV3788339.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            21536..24652
                     /locus_tag="VYS74_000246"
     CDS             21536..24652
                     /locus_tag="VYS74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV3788340.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            24709..26475
                     /locus_tag="VYS74_000247"
     CDS             24709..26475
                     /locus_tag="VYS74_000247"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV3788341.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASYAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNSDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            26621..27772
                     /locus_tag="VYS74_000248"
     CDS             26621..27772
                     /locus_tag="VYS74_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV3788342.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            27917..28801
                     /locus_tag="VYS74_000249"
     CDS             27917..28801
                     /locus_tag="VYS74_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3788343.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            29154..29405
                     /locus_tag="VYS74_000250"
     CDS             29154..29405
                     /locus_tag="VYS74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3788344.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            29393..29683
                     /locus_tag="VYS74_000251"
     CDS             29393..29683
                     /locus_tag="VYS74_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788345.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(29866..29993)
                     /locus_tag="VYS74_000252"
                     /pseudo
     CDS             complement(29866..29993)
                     /locus_tag="VYS74_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(30088..31419)
                     /locus_tag="VYS74_000253"
     CDS             complement(30088..31419)
                     /locus_tag="VYS74_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV3788346.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHKRSEIHIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(31441..32952)
                     /locus_tag="VYS74_000254"
     CDS             complement(31441..32952)
                     /locus_tag="VYS74_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV3788347.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(33163..34110)
                     /gene="arcC"
                     /locus_tag="VYS74_000255"
     CDS             complement(33163..34110)
                     /gene="arcC"
                     /locus_tag="VYS74_000255"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV3788348.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(34275..35291)
                     /gene="argF"
                     /locus_tag="VYS74_000256"
     CDS             complement(34275..35291)
                     /gene="argF"
                     /locus_tag="VYS74_000256"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV3788349.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(35349..36578)
                     /gene="arcA"
                     /locus_tag="VYS74_000257"
     CDS             complement(35349..36578)
                     /gene="arcA"
                     /locus_tag="VYS74_000257"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV3788350.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPVYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            37333..39012
                     /locus_tag="VYS74_000258"
     CDS             37333..39012
                     /locus_tag="VYS74_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV3788351.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTDAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGVEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGFPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLETKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(39052..41136)
                     /locus_tag="VYS74_000259"
     CDS             complement(39052..41136)
                     /locus_tag="VYS74_000259"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV3788352.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSLYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            41307..42587
                     /locus_tag="VYS74_000260"
     CDS             41307..42587
                     /locus_tag="VYS74_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV3788353.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSPEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            42678..45323
                     /locus_tag="VYS74_000261"
     CDS             42678..45323
                     /locus_tag="VYS74_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV3788354.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVCTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            45407..46276
                     /locus_tag="VYS74_000262"
     CDS             45407..46276
                     /locus_tag="VYS74_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3788355.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            46270..48150
                     /locus_tag="VYS74_000263"
     CDS             46270..48150
                     /locus_tag="VYS74_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3788356.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            48441..48803
                     /locus_tag="VYS74_000264"
     CDS             48441..48803
                     /locus_tag="VYS74_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788357.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(48855..49704)
                     /locus_tag="VYS74_000265"
                     /pseudo
     CDS             complement(48855..49704)
                     /locus_tag="VYS74_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(49649..49831)
                     /locus_tag="VYS74_000266"
     CDS             complement(49649..49831)
                     /locus_tag="VYS74_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV3788358.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            50166..>51552
                     /gene="pcpA"
                     /locus_tag="VYS74_000267"
     CDS             50166..>51552
                     /gene="pcpA"
                     /locus_tag="VYS74_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV3788359.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNRGV"
BASE COUNT        15785 a         9246 c        11125 g        15396 t
ORIGIN      
        1 gtcgtatatc tgaggtgtaa gtcctcggtg ggcacctgct accggtgaac ccaatagcga
       61 ttcccaagcc tgactatcgt gaggtagcag ggagaggaag ggatagcgaa atcgtggctc
      121 tacgaacagg aacgtgatag taaggcgtat atagcggata aggaggcttc aaactctaaa
      181 gtccaaaaag gtagtcgtaa cctatatgtg taaatcacga gagtaattga attcggacta
      241 aggtttgtgt gaaaaagata aatctttcta gagtctaaag actctgcgtc agatttccta
      301 ttttcactgt aaccttttaa cgtcctcata tcttgtataa acgaggaaag atgtacgact
      361 tatcccgtga ggtttcatga gcgctgaaag cgtagtaaca acgaatcatg agaagtcagc
      421 cgagcccata gtagtgagga aacttccgta atggaagtgg agcgaagggg tgaatactca
      481 aacagtctgg ggagagactg tttgaggtct gtcgctagaa agagaaaacg acagatcgaa
      541 gtaatcctac ttcacttgtg tctgtaaaac gaacggtctg atagaactgg actctgtcac
      601 gtaatgacta gatgaaggtt caccaatata agatgtccct caggcaccaa aacaagaaag
      661 gaataggcac atgtcaaaat tgctagataa aatattatca cgcgacaata tgcttgaagc
      721 ctacaatcaa gtaaaatcta ataaaggctc agctgggatt gatggaatta ctatcgaaga
      781 gatggatggc tatctcagac aaaactggcg cctgactaag gaactgataa aacagagaaa
      841 atataagcct caaccagttc ttagagttga gattcccaaa ccaaacggag gcatccgcca
      901 actaggaatt ccaacagtta tggatagaat gattcaacag gccattgttc aagttattag
      961 ccctatttgt gaaccccatt tctcagatac gagctatgga ttcagaccaa ataggtcatg
     1021 tgaaaaagcc atcatgaagc tcttagagta cttaaatgac ggctatgagt ggatagtgga
     1081 catagaccta gagaaatttt tcgatacagt tcctcaagat agattgatgt ccttagtaca
     1141 taacattatc gaagacggag atacggaatc cttgattcgt aaatatcttc attcaggtgt
     1201 tatcattaac ggtcaacgtt ataaaacgct agttggcacg ccacagggag gaaatttatc
     1261 tcctctctta tccaatatca tgcttaatga attggacaag gaattagaaa agagagggct
     1321 tcgatttgtt cgctacgcag acgattgtgt gattacggtc ggaagcgagg cagccgctaa
     1381 gcgtgtgatg tattcagtca gtcgttttat tgagaaacgg ctaggtctga aagtaaacat
     1441 gaccaagact aagattacca gaccaagaga gttgaaatat ctaggtttcg ggttctggaa
     1501 atcatcagat ggttggaaaa gtcgtccaca tcaagatagt gtttggagat ttaagcttaa
     1561 attgaagaaa ctaacacaga ggaaatggag tatagaccta acaagacgta ttgagcaact
     1621 gaatttgtct attcgaggat ggataaacta tttctcattg ggaaatatga aaagtatagt
     1681 cgccagcata gatgagcgct tgcgtactcg cctacgagtg attatctgga agcaatggaa
     1741 gaagaaatcg agacgattat ggggattgct taagttagga gttcctaaat ggatagcaga
     1801 taaggtatct ggctggggcg accattatca attagtagct cagaagtcgg tacttaaacg
     1861 tgctatatca aaaccagtcc tggaaaaacg tggactggtt tcgtgtttgg attattacct
     1921 tgaacgacat gcgttaaaag ttagttgaac cgccgtatgc cgaacggcac gtacggtggt
     1981 gtgagagggg ctagagatta tcccctactc gattaactcc cctgaaattt attttaatta
     2041 tgcaaatttc acgtattttt gatgctgaga cgacgatcct gggaactttt cagatatttt
     2101 tttgactatc taaatctatc attagaaaag cttagagcgc caaaggattt gagcgttttt
     2161 ctgattttta agactttttc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
     2221 taactaactt cttagtacta gccaacaacg ataatcataa ttcctcctaa aattaggaat
     2281 aataaaggca atagtttttg ttttttcatg taaaaaacct cacttttgtt ttctgctatt
     2341 ttatgctaaa atattaaaaa tcaaatttaa ttccaaagtt tgtaactaaa gggggagcgc
     2401 tacatgtcta attcatttgt caagttgtta gtctctcaat tatttgcaaa tttagcagat
     2461 attttcttta gagtaacaat cattgctaac atatacatta tttcaaaatc agtaattgcc
     2521 acatcactag ttcctatctt aataggaata tcctcttttg ttgcgagtct tttagttccg
     2581 ttggttacta aaaggttagc gctaaatagg gttttatctt tatctcaatt tggaaagact
     2641 atattattgg cgatactggt aggaatgttt accgtaatgc aatccgtagc gcctttggtg
     2701 acctatctat ttgttgttgc aatttccata ctagatggtt ttgcagcacc cgtttcctat
     2761 gctattgtgc cacgctatgc gaccgatttg ggtaaggcta attcagcctt atcaatgact
     2821 ggtgaagctg ttcaattgat aggttgggga ttaggtggac tcttgtttgc aacaattggt
     2881 ctgttaccta ccacgtttat cattttagtc ttgtatatca tttctagctt tctgatgtta
     2941 tttcttccta acgctgaagt ggaggtgtta gagtcagaaa ctaatcttga aattttgctc
     3001 aaaggttgga agttagttgc tagaaatcct agattaagac tttttgtatc agcaaattta
     3061 ttggaaattt tttcaaatac gatttgggtt tcttccatta tacttgtttt tgtaacggag
     3121 ttattaaata aaacggaaag ttactgggga tattctaata cagcatactc tattggtatt
     3181 ataattagtg gcttaattgc ttttaggcta tctgaaaagt tccttgctgc taaatgggaa
     3241 agtattcttt ttcctcttgt agctatggca atagtgacac tgactattct atattttcca
     3301 aacgcacaga tgtttttatt attttcagct ttagttggta tgttatcgca actagaagaa
     3361 gttcctgaaa gtgtatttct tcaggaaaca gtagaggaaa atcatttagt taatgtctat
     3421 tccgttcttg aagtgatttc tacattagca ttttcagtat ttgttttgct aatgagctat
     3481 attactgaga gttttggtat tagcatcagt ttttggctat cagccatttg tctgatgata
     3541 gaagctattt taatttatat cagacgagat tattttaaat gagagagtgg ttaaaaagtc
     3601 tttaaaaatc agaaaagcgc atagtatcag gtgttgaaaa aacttgatat tgtgtgtttt
     3661 attatgggaa gatttacttc attgtctcct gaaatggagt tttgaagcag gatcactcta
     3721 tttgaaaata tacatagaaa aaccgcataa tccttaaaat aagctgaaat ctattggaaa
     3781 catacggtaa acaattgaaa agatactata taaagtggta caatggtaga aaaataagct
     3841 agtaagaatc actcttattt tagcgaaaaa ttacagaaat gaatggtttt tcttgatgaa
     3901 acagagaaaa gaattgtacc tctttcttgg tcggacagcc ttgtattttc ttatctttct
     3961 agggctgctt tacttcttta gctatcttgg tcagggtcaa ggaagcttta tctataatga
     4021 attttaactt gaaggagaat ccctattgtg tcaaataaac caatagcaga tatgattgaa
     4081 accattgagc attttgctca gacacagcct agctatcctg tctataatgt tttggggcag
     4141 gaacacactt atggcgattt aaaggctgat tcggatagtt tggctgcagt cattgaccaa
     4201 ctaggcttgc ctgagaagtc tcctgtggtt gtttttggtg gccaagaata tgaaatgttg
     4261 gcaacctttg tagcgctgac taagtcaggt catgcctaca ttccaattga tagccattcg
     4321 gccttggagc gagtttcagc tattttagaa gtagcagagc caagcttgat tattgccatt
     4381 tcagcctttc ccttggagca ggtttctaca ccaatgataa atctagctca ggttcaagaa
     4441 gcctttgccc aagggaacaa ctatgaaatc acgcatccag tcaagggaga tgataattac
     4501 tacattatct ttacttctgg tacgactggt aagcctaagg gagtgcagat ttcacatgat
     4561 aatctcctca gctttacaaa ctggatgatt acggataagg aatttgcgac accgagtcgt
     4621 ccgcaaatgc tggcacagcc accttattct tttgacttgt ctgtcatgta ttgggcaccg
     4681 accttggcac ttggtggtac gcttttcact cttccttcag tcatcactca ggactttaag
     4741 caactctttg cggctatctt ttcattgcca atcgctatct ggacatcaac accatccttt
     4801 gcagatatgg ccatgttgtc tgaatacttc aacagtgaga aaatgcctgg aatcacgcat
     4861 ttctactttg atggtgaaga attgacggtc aaaacagctc aaaaactgcg cgagcgtttc
     4921 ccaaatgccc gtatcatcaa tgcttacggc ccaacagaag cgacagtagc tctgtcagca
     4981 gttgccgtta cagacgagat gttagcgact ctcaaacgcc taccaatcgg ctataccaag
     5041 gctgattctc caacctttat cattgacgag gaaggaaata aactgccaaa tggcgaacag
     5101 ggagaaatca ttgtttctgg tccagctgtt tcaaaaggtt atatgaacaa tcctgaaaaa
     5161 acagcagaag ccttctttga gtttgaaggt ctgccagcct atcacacagg cgatgtggga
     5221 accatgacag atgagggctt gcttctctac ggcggacgca tggacttcca gattaagttt
     5281 aacggttacc gcattgagtt agaagatgtc tctcaaaacc tcaacaagtc tcgctttatc
     5341 gaatctgctg tcgcagtacc gcgctataac aaggaccaca aggtacaaaa tctattggct
     5401 tatgtcatct taaaagacgg tgttcgtgag cagtttgagc gagatatcga tattaccaag
     5461 gccatcaagg aagacctgac agacatcatg atgtcctaca tgatgccatc taaattcctt
     5521 taccgagaca gtttgccact aactccaaat ggaaagattg acatcaaagg attgattaac
     5581 gaggtgaata agagatgatg gagttttttc aacagcttcc tcatttagag ccatatggca
     5641 atcctcagta ttttgtttat gtgattgctg caaccttgcc catctttata ggtctctttt
     5701 tcaagaaacg ctttgcctgg tatgaagtgt tggtaagtct cttctttatt gtcaccatgt
     5761 tggtgggtgg aaagaccaat caactagctg ccttgggtat ttacctttgc tgggaaatat
     5821 tgctcctgct tttctacaag cattatagaa aaagcaagga tggcaagtgg gtcttctact
     5881 tagttagttt tctgtcccta cttccgatta tctttgtcaa ggtgcaacca gctatcaatg
     5941 gaacgcagtc tttgcttggg ttcttgggaa tttcttacct gacatttcgt tcggttggga
     6001 ttatcatcga gctgagagat ggagtgatta aggattttac cctctgggaa ttcctccgtt
     6061 tccttctctt catgccaact ttctcgagtg gtccaatcga tcgttttaag cgatttaatg
     6121 aaaattatca ggccattcct gagcgagatg agttgatgga tatgctggat gaatctgtcc
     6181 gctatatcat gcggggcttt ttgtataagt ttatcctagc tcatgtttta ggagagacct
     6241 tactacctcc tctgaagaat ttagccttgc agtcaggtgg cttctttaat ctctatgcct
     6301 tggcagttat gtatactttt ggtctggagc tcttctttga ctttgcaggt tattctatgt
     6361 ttgctttggc catctcaaac ttgatgggaa tccgtagccc tatcaacttt aacaagccct
     6421 ttttatcaag ggatttaaag gagttttgga atcgctggca tatgagtctg tccttctggt
     6481 tccgtgactt tgtctttatg cgaatggtga tggtgttaac cagaaagaaa gtctttaaaa
     6541 atcgtaatgt aacctcaagc atggcctaca ttgtaaatat gctgattatg ggattttggc
     6601 atggtgtgac ctggtactat atcgcctatg gactctttca tggactaggc ttggtcatca
     6661 atgatgcctg ggttcgcaag aaaaaaacgc tcaataagga acggaaaaaa gcagggaagg
     6721 ctgccctacc tgagaatcgc tggattcagt tgcttggcat ggttatcact ttccatgttg
     6781 tcatgttgtc attcttaatc ttttctggat tcttgaataa tctatggttt aaaaaataaa
     6841 aggaaataaa atatggatat caaatcagaa gttattgaaa ttattgatga gttgtttatg
     6901 gaagatgttt ctgacatgat ggatgaagat ctttttgatg caggtgtctt ggatagtatg
     6961 ggaacggttg agttgattgt ggagattgag aaccgttttg acattcgtgt ccctgtaaca
     7021 gagtttggtc gtgacgactg gaatacagct aataaaatca tagctggtat tgtggagcta
     7081 caaaatgctt aaacgcttat ggatgatctt cggaccggtc ttgatcgctg gtttgttggt
     7141 ttttctgctc attttctttt atcctactga gatgcatcat aatctaggag ctgaaaagcg
     7201 ttcagcagtg gctactacta tcgatagttt taaggagcga agtcaaaaag tcagagcact
     7261 atctgatcca aatgtgcgtt ttgttccctt ctttggctct agtgaatggc ttcgttttga
     7321 cggtgctcat cctgcggtat tagctgagaa atacaatcgt tcctaccgtc cttatctttt
     7381 aggacagggg ggagctgcat cgcttaacca atattttgga atgcaacaga tgttaccaca
     7441 gctggagaat aaacaagttg tgtatgttat ctcacctcag tggttcagta aaaatggcta
     7501 tgatccagca gccttccagc agtattttaa tggagaccag ttgactagtt ttctgaaaca
     7561 tcaatctggg gatcaggcta gtcaatatgc agcgactcgc ttactgcaac agttcccaaa
     7621 cgtagctatg aaggacctgg ttcagaagtt ggcaagtaaa ggagaattgt cgacagcaga
     7681 caatgaaatg attgaattat tggctcgttt taatgaacgc caagcctcct tttttggtca
     7741 gttttcggtt agaggctatg ttaactacga taagcatgta gctaagtatt taaaaatctt
     7801 gccagaccag ttttcttatc aagcaataga agatgttgtc aaagcagatg ctgaaaaaaa
     7861 tacttccaat aatgagatgg gaatggaaaa ttatttctat aatgagcaga tcaagaagga
     7921 tttgaagaaa ttaaaggatt ctcagaaaag ctttacctat ctcaagtcgc cagagtataa
     7981 tgacttgcag ttggttttaa cacagttttc taaatctaag gtaaacccga tttttatcat
     8041 tccacctgtt aataaaaaat ggatggacta tgctggtcta cgagaggata tgtaccaaca
     8101 aacggtgcag aagattcgct accagttaga aagtcaaggt tttaccaata tagcagattt
     8161 ttctaaggac ggcggggagc ctttctttat gaaggacacc attcaccttg gttggttggg
     8221 ttggttggct tttgacaagg cagttgatcc tttcctatcc aatcccacac cagctccgac
     8281 ttaccatctg aatgagcgct ttttcagcaa agattgggcg acttatgatg gagatgtcaa
     8341 agaatttcaa tagataatat aatatagaag agttcaagaa tgaaactagt tttcacgttt
     8401 tttcttgact ctttttttgc ttgtgatata attaactggt aaacaaaatg tcaaagaata
     8461 gtatttttct cttaaaaaag agaagtctaa aaggaaagga gtcagatatg agacagctag
     8521 caaaggatat caatgctttt ttgaatgagg tgattttgca ggcggaaaat cagcatgaaa
     8581 tcctaatagg tcattgcact agcgaggtgg ccctgaccaa tactcaggag catatcctta
     8641 tgctcttgtc agaggaatct ttaacaaatt cagaattggc ccgtcgtctc aatgtcagtc
     8701 aggcggcagt taccaaggcc attaagtctt tggtcaagga agggatgttg gaaacatcta
     8761 aagattctaa agatgcgcgt gtgatttttt atcagttgac tgacttggct cgtccaatcg
     8821 ctgaggagca ccaccatcac catgagcata cacttttaac ctatgaacaa gtggcgactc
     8881 agtttactcc aaatgaacaa aaagtgattc agcggttttt gactgcttta gtaggagaaa
     8941 tcaaataatg agatatatta cggtagagga tttgtccttc tattatgata aggagcctgt
     9001 tcttgaacat atcaattatt gtgttgatag tggggaattt gttaccttga ctggggaaaa
     9061 tggagcggct aagacgacgc tcatcaaggc tagtcttgga attctgcaac cacgcattgg
     9121 aaaggtggct atttcaaaga caaatacgca aggtaagaaa ttgagaatag cctatcttcc
     9181 tcaacaaatt gccagtttta atgctggttt tccaagtacg gtctatgaat ttgtcaagtc
     9241 gggtcgctat ccgagaaaag gctggttccg tcgtttgaat gctcatgatg aggagcatat
     9301 caaggctagt ctggactcag ttggcatgtg ggaacatcga gacaaacgct tggggtctct
     9361 atctggggga caaaagcagc gagcggtaat tgcgcgtatg tttgcttctg accctgatgt
     9421 gtttatccta gacgagccga caacggggat ggatgcagga agtaaaaatg aattttacga
     9481 actcatgcac cacagcgccc atcatcatgg taaggctgtt ttgatgatta cccatgaccc
     9541 tgaagaagtt aaggattatg cggatcgcaa tattcatcta gtccgtaacc aagactcgcc
     9601 atggcgttgt ttcaatgttc atgagaatgg ccaggaggtg ggccatgctt agtttattat
     9661 cttacgactt tatacagcgc gcctttttgg cggttattgc tatgagtctt ttctcaccgg
     9721 tattgggaac cttcctcatc ttgcgtcgtc agagtttgat gagtgatacc cttagccacg
     9781 tctcactttc aggtgtagcc tttggtctgg ttttggggat ttctccaact gtttctacta
     9841 ttgccattgt cttgattgcg gcggtctttc tggagtatct ccgtacggtt tacaagagct
     9901 ttatggaaat cgggacagct atcctcatgt caacaggtct ggctgtttct ctgattgtca
     9961 tgagcaaggg taaaagctcg agttcaatga gtttggacca atatctcttt ggttcgatcg
    10021 tgactatcag tgaagaacag gtcatttccc tctttgtcat tgcggcggtt gttttgattt
    10081 tgacctttct ctttcttcgt cctatgtata tcttaacttt tgacgaagat acggcctttg
    10141 tggatggctt gccagttcgt accatgtcca ttctttttaa catggtgaca ggggtggcta
    10201 ttgcccttat gattcctgca gcaggagctc ttctggtatc gaccattatg gtcttgccag
    10261 ctagtattgc cctgcgtctg gggaaaaact ttaaatcggt tatgctgctt gccagtgcga
    10321 ttggcttttt gggaatggta gcaggacttt acatttccta ctatgcagaa acacctgcaa
    10381 gtgcaagtat tactattatt tttgtaactg tctttatact aatcagttta gtaagacgtt
    10441 ttatcaaata ggagacgaac gtgaaaaaaa ttagcttatt actagccagt ctatgtgcct
    10501 tgtttttagt ggcttgttcc aatcaaaaac aggcagatgg taaactcaat atcgtgacaa
    10561 ccttttaccc tgtctatgaa tttaccaagc aagtcgcagg agatacggct aatgtagaac
    10621 tcctaatcgg tgctgggaca gaacctcatg aatacgaacc atctgccaag gcagttgcca
    10681 aaatccaaga tgcagatacc ttcgtttatg aaaatgaaaa catggaaaca tgggtaccta
    10741 aattactaga taccttggat aagaaaaaag tgaaaaccat caaggcgaca ggcgatatgt
    10801 tgctcttgcc aggtggcgag gaagaagagg gagaccatga ccatggagaa gaaggtcatc
    10861 accatgagtt tgacccccat gtttggttat caccagttcg tgccattaaa ctagtagagc
    10921 acatccgcga cagcttgtca gcagattatc ctgataaaaa agagaccttt gagaagaatg
    10981 cagctgccta tatcgaaaaa ttgcaagcct tggataaggc ttacgcagaa ggcttgtctc
    11041 aagcaaaaca aaagagcttt gtgactcaac acgcagcctt taactatctt gccttggact
    11101 atggactcaa acaagtcgca atctcaggac tttctccaga tgcagagcca tcagctgctc
    11161 gcttggcaga attgacagag tatgtcaaga aaaataaaat cgcctatatc tattttgaag
    11221 aaaatgcctc acaagccctt gctaacacac tttcaaaaga agcaggtgtc aaaactgatg
    11281 tcctcaatcc tttagaaagt ctgacagaag aggacaccaa ggctggagaa aattacatct
    11341 ctatcatgga gaaaaacctc aaggctttga aacaaacaac agaccaagaa ggcccagcga
    11401 tcgagcctga aaaggcagag gataccaaga cagtccaaaa tggttacttc gaggatgcag
    11461 ctgtcaagga ccgcaccttg agtgactatg caggtaactg gcaatcagtt tatcctttcc
    11521 ttgaagacgg cacgtttgac caagtctttg actacaaggc taagttgact ggtaagatga
    11581 cccaggctga gtacaaggct tactatacaa aaggctatca gacagatgtg actaagatta
    11641 acattactga taatactatg gaatttgttc aaggtggaca aagcaagaaa tatacttaca
    11701 agtatgtcgg taagaaaatt ttgacttaca agaaaggcaa tcgtggcgtg cgtttcctct
    11761 ttgaagccac agatgctgac gctggacaat tcaagtatgt tcagtttagt gaccacaata
    11821 tcgccccagt taaggcagaa catttccata tcttttttgg aggcacaagc caagaagccc
    11881 tctttgaaga aatggacaac tggccaacct actacccaga taacctatct ggccaagaaa
    11941 tcgcccaaga aatgttagcg cattgatgag aaatcgacta gttcataaga gctagtcggt
    12001 tttttgatac tagatggcta acttcattat agacctttca gaactaaggg aacctgaaaa
    12061 aataaataaa actcttgaaa tatctgggct tatatgctag aatgtagtta gtgtataagg
    12121 ttcattagaa caccaaagcc cctaactagt gccatagtta agggcttttt tgacgtatct
    12181 tagcgattta acgggtgttt ataggctagt cagtcggtct acttcttacc atctagccat
    12241 ttgcagacga aatacaagat aattccagct ataacatctg ctataatagt aagagcaagc
    12301 tctggtataa ggctcattag tttcacctcc tctcacgaac ccataggaac gtaatcggta
    12361 accgatgaca aaaatagtat accacactaa atttagatca tcaacctcac ttaatgcttg
    12421 aactattgta gattatatgc taaaatgtag ttaattgata aggttcatta gaacaccaaa
    12481 gcccctaact atggccgtag ttaagggctt ttttgacgta tcttagagat ttaacgggtg
    12541 tttataggct agtcagtcgg tctactcctt actatctagc catctgcaga cgaaatacaa
    12601 gataattcca gctataacat ccgctataat agtaagagcg agctctatga taaggctcat
    12661 tagtttcacc tcctctcacg aacccatagg aacgtaatcg gtaaccgatg acaaaaatag
    12721 tataccacaa tacatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc
    12781 gacaaagtct ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat
    12841 gcgttttatt gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct
    12901 ctgtttttgg ggttgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc
    12961 agacagaacg atactcttcg aaaatctctt cacatcatgt cagcttcgtc tttccgtata
    13021 tatgtgactg acttcatcag ttctatctac aacctcaaaa cagtgtttcg agctgacttg
    13081 atcaattttc aaatctgtac tgtgagcaag ctgagactag cttcctattt gattttcatt
    13141 gaatatcaga aacccattct ccatcaaata attcgactgc gtctaataat ttttgatctg
    13201 gcacggtgtc tgaaataaag gttgtgtatt tggagagggg attaatttta aaaaatccag
    13261 tcttgtaaaa tttagaacta tcaatcagta agatggtttc atgggctttg tcaataatat
    13321 tcttttttga aatagcttgg ctgagagaag cttcataaac atattggtca tcaatacctc
    13381 ttgctgaaca aaatgctaaa tcgatattaa aatgatctaa taaagaattt tccttatcgt
    13441 aattgaccac ggaacaggat tgatgtttga cctcgccaga tgtgataaag attttggagc
    13501 tatctttaac agtttcagat agggtttgtg cagtatgtaa accatttgta aaaataatca
    13561 aattatcaag ttcagaaaga tagggacaaa gttcgtagac agtcgtactg gaatccagat
    13621 aaacacacat acctgaccga ataaagtctt tagcgagact agcgattagt cttttttgcc
    13681 tagtactttc tccttcccgt atttgatgag aaagttcaat tgtgttcata gaggacaggg
    13741 tcacgtatcc gtgctttctt ttgataagac cttgattttc taagaaaatt aaatcacgac
    13801 gtaaggtact tgtgctggag aaagtgattt ctgccagctc ttttacggca attctttttt
    13861 tctttttaat aatttcaatc aattcaagta cacgttcatc ttttatcata agctcctcct
    13921 aatttatcat ttcaactata ttatagcaca aattggagga atttgaatta tttttatgaa
    13981 tattgggtta acatttgaac attatccaag taagcgttca catattgaaa aaataaaatg
    14041 tggggattat aataaactta atcaaggacg aagagagaag aaaaatggaa gcggttttag
    14101 caatagattt aggtgcgact tctggaagag caatcgttgg ttacctttct gaaaataaac
    14161 tagtaatgga agaaataaat cgcttttcta atctacctat tagagtaaaa gggcatttat
    14221 cttgggatat tgactttcta ctagctaaaa ttcttgaaag tatccgcttg gctaatacta
    14281 gttacaagat tttatctatc ggtattgaca catggggagt tgattttgga ctgattgata
    14341 atgaaggtaa gctgttatca caacctgttc attatcgtga tgaaagaaca aagggagtgt
    14401 taaaggaaat atctgaaatg actgaattag aaaaactgta ttcagagaca ggaaatcaga
    14461 ttatggagat aaataccttg tttcaactct ttaaggcacg tcaagaatct cctgactctt
    14521 tctataagac caataagatt cttttaatgc cagatttgtt taattatctc ttgacaggta
    14581 agtttgctac agaaaaaagc attgcttcaa caactcaatt atttgatcct aggagtcaaa
    14641 attggaatca gaatatctta aaactatttg aattggattc atctttactt cctgaaattg
    14701 tttcagaggg aaatgttctt ggaaggataa aaaaggagta tggtttaggc gatattcctg
    14761 ttgtgaatgt ttgtagtcat gatacagcaa gtgcgattgt ctcagtacct aagacagaag
    14821 gtagtttatt tatttcatca ggtacttggt ctttggttgg agtggaactt acttcaccga
    14881 ttcttactac cgaatccttc agttatggat ttacaaatga agtcggtaaa gatggagtga
    14941 ttacatttct gaagaattgt acagggttgt ggatcataga ggaactaaga cgttcatttg
    15001 aacgaagagg gaaagcctat tcttttgatg atattaggac aatggtggag aaagaaaaag
    15061 aaaatcttcc tctgattgat actgaatcaa ctgaatttgc aacagaatct gatatgcaca
    15121 agactttgac agaatatcta gcttatcatc atgaaactag agagtggaca gatggacaac
    15181 tatttaagat tgtttatgaa agcctagctg aaacgtatag gaaagcgata gagttactag
    15241 aagaactaac tcataaggtt tataagagga tatatgtgat tggaggaggt gctagagcca
    15301 gttactttaa ccaaatgatt gctgatagaa ctggtaaaga ggttcttaca ggttcgactg
    15361 agggtacagc tgtggggaat attgttgtgc agctcatagc tatgggacaa ttaaaaggga
    15421 tggaagaagc tcaccatgtt attgaggagt ttctacaatt agagagttat tactcccaaa
    15481 agaattaaaa agattgagag tttgtaaatt tgcctccctc ccccttctta agttttgtac
    15541 aagaaggggg gataattggt gaattgaaaa atatttagtg ttttgatatg aggaggacaa
    15601 gaatgtcaga tgtaaaacaa gaattaatta agtatggcaa gaagctagta gaaacagatt
    15661 tgacgaaagg aacaggtggg aatctcagcg ttttcgatcg tgaaaaacaa ttgatggcaa
    15721 ttaccccgtc gggtattgat ttctttgaaa tcaaagaatc cgatattgta gtgatggata
    15781 ttaatggaaa tgttgtagag ggagaacgct tgccatctag cgaatggtat atgcatttga
    15841 ttcaatatca aactcgtgat gatatcgatg caattatcca tgctcataca acttatgcaa
    15901 cagtattagc ttgtctcaga gaaccacttc cggctagtca ttatatgatt gcagtggcag
    15961 ggaaagatgt tcgggtagct gagtatgcaa catatggtac gaaagaattg gctgtgaatg
    16021 cagctaaagc aatggaaggt cgtagagcag ttttactagc gaatcatgga attttagcag
    16081 gtgcacaaaa tttattgaat gcatttaata ttgttgaaga agttgaatat tgtgcaaaaa
    16141 tttattgttt agctaagaat tttggagagc cagtagttct tcctgatgag gagatggatt
    16201 taatggcaga aaaatttaaa acatacggtc agagaaaata gggaggatat taatgttaaa
    16261 acatataccg aaaaatattt ctccagattt attgaagact ttaatggaaa tgggacatgg
    16321 agatgaaata gtattagctg acgcgaatta tccttctgcc tcatgtgcaa ataagctaat
    16381 tcgttgtgat ggcgtaaata ttccagaatt attagattcc attctgtatt taatgccatt
    16441 agatagttac gtcgatagtt caattcagtt tatgaacgtt gtttcgggtg atgatattcc
    16501 taagatatgg ggtacctata gacagatgat tgaaggtcat ggtacagatc ttaaaacgat
    16561 tacttatctt agaagagaag acttttatga acgtagtaag aaagcttatg ctattgttgc
    16621 tacaggagaa acttcacttt atgctaatat tatccttaag aaaggagtag ttgttgaaag
    16681 agaaaatgtt taatagagga attttagttg ccagtcatgg taattttgct agcggagctc
    16741 tcatgaccgc agaaatgttt gttggtgaga caacaaatga tagagttagg acattaggtt
    16801 tgatgcctgg agagaatatt gtagagtttg aacattattt taaaaatcaa gtaaatgaac
    16861 tgttagactc aaatcaagag gttatcgttt tgactgactt gattggagga agtcctaata
    16921 atgtggcttt gtcacggttt ttaaatttgg attcagttga tattgtaaca gggtttaata
    16981 tccctctcct agtggaatta atatcaagtt atgattcaaa aatcaattta gaagaaattg
    17041 ttcaaaatgc tcaaaatagt ttgtttaatg ttaaacaaca acttaacgta gaggaggaag
    17101 aggatttatg tctatagagt ttgttcgtat tgatgaccgt ctggtacatg gtcaagttgt
    17161 cactacgtgg ctaaaaaagt atgatattga gcaagttatc attgttaatg atcgcatctc
    17221 agaagataaa acacgacaat ctattttaaa gatttctgca ccggtaggtt taaaaattgt
    17281 tttctttagt gtaaaacggt ttgtggaagt tttaaactct gtgccaataa aaaagagaac
    17341 aatgctgata tatacaaatc caaaagatgt gtatgattct attgaaggaa atttaaaatt
    17401 ggagtacctc aatgtaggac agatgagtaa aacggaggaa aatgaaaagg taacgggagg
    17461 tgtagctcta ggtgaagaag acaaatatta ttttaagaaa atagttgata agggaacgag
    17521 agttgaaatt caaatggttc ctaatgataa agttacaatg ttggaaaaat ttttataaaa
    17581 ataatttaag gaggtacagt atatgctatt cacacaagca ttactggtga cattagttgg
    17641 gattattgcc actattgact ataatggacc gttatttatg attcaccgtc cgttagttac
    17701 aagtgcaatg gttggcttag tcttaggaga tttcacccaa ggtgttctta ttggttcagc
    17761 tcttgaatta acttggctcg gtgtaacagg tattggaggt tacactccac cagatactat
    17821 ttcaggtgcg attattggta ctgcatttgg tattttatct ggtcaaggag aaactgctgg
    17881 tatcgctata gcagttccaa ttgcagttgc tacccaacag ttggatgttc ttgcaaaaac
    17941 tttagatgtt tattttgtga aaaaagctga taatgatgct aaaaacggag attattcaaa
    18001 gatcggtttt tatcattatt caagtttggt tttaatcacg ttatttaaaa ttgtaccaat
    18061 tttcctagct attatgcttg gaggggaata tgtggcagac ttgtttgcta aggttccacc
    18121 aatcgttatg cagggactta actctgcagg tgctttacta ccttcaattg gttttggtat
    18181 gcttttaaat atgatgctca agaaaaatat gtgggtattc ttgttgattg gattcatttg
    18241 ttctgtgtat ggaggaatgt caaccattgg gatctcacta gttggtattg cggtagcata
    18301 cttctacgat atgattggaa gtaaaccaca agaaacaact tcaagtagtg atgttgagga
    18361 ggatcttgat ctatgatgaa taataaagta actaaagttg aacttaaaaa agttttcaaa
    18421 cgaagtttta tgtatggttc ttcatggaac tatgagagaa tgcagaacct aggttttcta
    18481 tatacaattc ttccagtatt gaaaaaacta tacccagaca aagattcagc ttctcctgca
    18541 atgaaacgtc accttgagtt tttcaatact catcaaacag cggcaccatt tattcttgga
    18601 gttacttccg ctatggaaga acaagaagga aatgaaggtg cagcttcaat tactggtatt
    18661 aaagttggct tgatggggcc actggctggt ctaggagata gtttgttctg gctgacacta
    18721 gttcctatct gttttagtat tggtgcgtct tattctaaag acggcggtgc tttaggtatc
    18781 tttatcgcct taatattgtt taatattatt aatattcctg ttaaatattt cggtttgaaa
    18841 tatgggtata ctaagggttc tagtcttatc caagaaaata atacaaaagg aacattgaat
    18901 cgcgttacga gtatggcgac agcattaggg ctagtactag tgggtggttt gattccatca
    18961 atggttggta ttaattttgg attagaattt aagcaggggg aacttgttat ttctgttcaa
    19021 gaaatgatta caaaattaat tccaggattt atccctatgg ctttgacttt attaatgtgt
    19081 aaattaatta gaaaaggaaa gaatccggtt gtactaatct ttagtgttat ggctattgga
    19141 gttatcctag ttgttttagg aattttgaag tagtagaaag tgtggaggtg gtatttggga
    19201 tatcacctcc attttggaag agaggtaaag agtgaaatta tggtataaga aagctgcctc
    19261 aaattggaat gaagccttgc cgattgggaa cggtcattta ggtggtatga tttatggttc
    19321 agctacaaaa gaatgtattc aactaaacga tgagactatt tggtatagag gaaagtcaga
    19381 tagaaataat ccagactcac tattgcatct taaaaaaatt cgggaatatc ttttagatgg
    19441 agaaattcag aaagccgaag aattgataaa gttaacaatg tttgctaccc caagagatca
    19501 aagccactat gaattacttg gggaacttta cattgagcat atagatattc agtcttgtgc
    19561 tctttcattg tatgaaagag agctagattt agatacagct atttctaatg ttgtgtttga
    19621 gcctaatagt tgtaatttac aaataaaaag agaatatttt acgagtttta ataagaatat
    19681 tttatgttgc cgtatagtgt catcagttca aaacacatta aatttaaaca ttaatttggg
    19741 tagaaataaa cggtttaatg atgaagtatc taaactggat tcaagtacaa ttttaatgtc
    19801 ggcctctgct ggaggtagaa aaggtgttca gtttaaagta gtatgtcatt ctaaggttac
    19861 ggatggtgaa gtaagtgtat tgggagagac aatagttatt cggaatgcta cagaggtatt
    19921 tctttatctc aaatcaatga cggattattg gggaaatata gatatttctt ctcttcaggg
    19981 agaatttagt agtattgatt actttacaga aaaagatgaa catgtaaaaa aatatcagga
    20041 gcaatttaat agagttgatt ttaaactaga ctatagtaaa gattgtctta gcattccaac
    20101 gaatctactt cttgaaaaca ctaaaaagta tagtaactac ttgactaact tgttatttca
    20161 ttatggaaga tatctgttaa tatcgtctag tcaaccgaat ggtttacctg ccaatcttca
    20221 aggaatatgg tgtgatgaat taaatccaat ttggggttct aaatatacga ttaatattaa
    20281 tactcaaatg aattattgga tggtaggtcc atgtgattta ccagaagtag aatatccatt
    20341 atttgatatg ctcgaaagaa tgagagaacc gggaagacta accgctaaga aaatgtatgg
    20401 agctagaggt tttacagcac atcataatac ggatggtttt ggcgatacgg ctccccaatc
    20461 tcatgccatg ggggctgcaa tttgggtatt aactattcca tggttatgta ctcatatttg
    20521 ggaacactat ttatatttcc aagatgagcg tattcttacg gaacattttg aaatgataaa
    20581 agaagcattt cttttctttg aagattattt atttgaggtg gatggctact tgatgacagg
    20641 tccaagtgtc tcaccggaaa ataaatatcg cttaaaaaat ggtattgaag gaaatgcttg
    20701 tctatcatct acaattgata atcaaattct aagatatttt tgtgattcat gcattggcat
    20761 tgcaaaacaa ttaggagaca attcggattt tattagtcgt gtgaaggagt taaaaaagaa
    20821 actacctaaa acaaaaatag gtagtaatgg gcaaatccaa gaatggttag aagattatga
    20881 agaagtagag cctgggcata gacacatttc acctctattt gggctttatc cttataatga
    20941 gattgatatt cataaaactc cggaattagc agaagcagct aaaatcacta tcaataggag
    21001 attatcaaac gctaattttt tatcttcaca ggagagggag caagcgatta ataattggtt
    21061 agtaagtggt ttgcatgcta gtacacaaac aggttggagt gctgcatggc tgattcattt
    21121 ttttgcgaga ctatatcaag gtgaacctgc ttataaccag attaatggtt tgttaaataa
    21181 tgcgactctt ggcaatttat ttcttgacca tccaccattt caaattgatg gtaatttagg
    21241 tttggtgagt ggaatttgtg aattattagt acagagccat cataattggt tatcactaat
    21301 tccagcttta ccttctgctt ggtcagaagg agaagtgaaa ggtttcagag taagaggagg
    21361 atataaggta tcgtttgctt ggaaaaatgg ggatataaca ttcctaaaat tggaaggagg
    21421 aaacaaagat caaaaagtaa gagtaagaat atatggcaaa aatactgatg tacaaaatat
    21481 tgaattggta tttaattcag aaaaaattat tgagttaaat ttttaggtat aagtcatgaa
    21541 taaagaaaaa ataaaaagaa aattaatcac aatactgttt gtatgtattg ggatgttatg
    21601 ttttggattg ttagcaggag ttaaggctga taatcgtgtt caaatgagaa cgacgattaa
    21661 taatgaatcg ccattgttgc tttctccgtt gtatggcaat gataatggta acggattatg
    21721 gtgggggaac acattgaagg gagcatggga agctattcct gaagatgtaa aaccatatgc
    21781 agcgattgaa cttcatcctg caaaagtttg taaaccaaca agttgtattc cacgagatac
    21841 gaaagaattg agagaatggt atgtcaagat gttggaggaa gctcaaagtc taaacattcc
    21901 agttttcttg gttattatgt cggctgggga gcgtaataca gttcctccag agtggttaga
    21961 tgaacaattc caaaagtata gtgtgttaaa aggtgtttta aatattgaga attattggat
    22021 ttacaataac cagttagctc cgcatagtgc taaatatttg gaagtttgtg ccaaatatgg
    22081 agcgcatttt atctggcatg atcatgaaaa atggttctgg gaaactatta tgaatgatcc
    22141 gacattcttt gaagcgagtc aaaaatatca taaaaatttg gtgttggcaa ctaaaaatac
    22201 gccaataaga gatgatgcgg gtacagattc tatcgttagt ggattttggt tgagtggctt
    22261 atgtgataac tggggctcat caacagatac atggaaatgg tgggaaaaac attatacaaa
    22321 cacatttgaa actggaagag ctagggatat gagatcctat gcatcggaac cagaatcaat
    22381 gattgctatg gaaatgatga atgtatatac tgggggaggc acagtttata atttcgaatg
    22441 tgccgcgtat acatttatga caaatgatgt accaactcca gcatttacta aaggtattat
    22501 tcctttcttt agacatgcta tacaaaatcc agctccaagt aaggaagaag ttgtaaatag
    22561 aacaaaagct gtattttgga atggagaagg taggattagt tcattaaacg gattttatca
    22621 aggactttat tcgaatgatg aaacaatgcc tttatataat aatgggagat atcatattct
    22681 tcctgtaata catgagaaaa ttgataagga aaagatttca tctatattcc ctaatgcaaa
    22741 aattttgact aaaaatagtg aggaattgtc tagtaaagtc aactatttaa actcgcttta
    22801 tccaaaactt tatgaaggag atgggtatgc tcagcgtgta ggtaattcct ggtatattta
    22861 taatagtaat gctaatatca ataaaaatca gcaagtaatg ttgcctatgt atactaataa
    22921 tacaaagtcg ttatcgttag atttgacgcc acatacttac gctgttgtta aagaaaatcc
    22981 aaataattta catattttat tgaataatta caggacagat aagacagcta tgtgggcatt
    23041 atcaggaaat tttgatgcat caaaaagttg gaagaaagaa gaattagagt tagcgaactg
    23101 gataagcaaa aattattcca tcaatcctgt agataatgac tttaggacaa caacacttac
    23161 attaaaaggg catactggtc ataaacctca gataaatata agtggcgata aaaatcatta
    23221 tacttataca gaaaattggg atgagaatac ccatgtttat accattacgg ttaatcataa
    23281 tggaatggta gagatgtcta taaatactga ggggacaggc ccagtctctt tcccaacacc
    23341 agataaattt aatgatggta atttgaatat agcatatgca aaaccaacaa cacaaagttc
    23401 tgtagattac aatggagacc ctaatagagc tgtggatggt aacagaaatg gtaattttaa
    23461 ctctggttcg gtaacacaca ctagagcaga taatccctct tggtgggaag tcgatttgaa
    23521 aaaaatggat aaagttgggc ttgttaaaat ttataatcgc acagatgctg agactcaacg
    23581 tctatctaat tttgatgtga ttctatatga caataataga aacgaagttg ctaagaaaca
    23641 tgttaataat ttgtcgggtg aatctgttag tctagatttc aaagaaaaag gagcaaggta
    23701 tattaaagtt aaattactaa cgagtggagt gcctttgagt ttagcagaag tagaggtttt
    23761 tagagaatca gatggtaagc aatctgaaga ggatatagat aaaataacag aagataaagt
    23821 agtctctaca aataaggtag ctactcaaag ttcaaccaat tatgagggtg tagctgcttt
    23881 agcagttgat ggtaataaag atggagatta cggacatcat tcggtgactc atactaaggc
    23941 agattctaac gcttggtggc aggtcgatct gggagaagag tttacggttt ctaaagttga
    24001 tatttataat agaacagatg ccgaacctca gcgtttatct aattttgatg ttatttttct
    24061 atcttcatca ggagaagaag tttttagaag acattttgat aaagtagttg atggtttgtt
    24121 atctttaaaa gtaccttctg taggggctaa gctagtcaaa atagaattaa aatcagcagc
    24181 tattccgtta agtttagcgg aagttgaagt ctatggttca aagagaactc cgaagaaact
    24241 ttctaatatt gcattaacca aagaaactcg acagagttca acggattaca atggtttttc
    24301 tcgtctagca gttgatggaa ataaaaacgg agattatggt catcattcag tgactcatac
    24361 caaaggagat tctccttcat ggtgggagat agatttagca caaaccgaag aattagaaaa
    24421 gttaattatt tataatagaa cagatgctga aattcagaga ttatcaaatt ttgatattat
    24481 tatatatgat tcaaataatc atgaagtttt taaacaacat attgacagtt tagaaagcaa
    24541 taatctatcc atagacttaa aagggctgaa gggaaaaaag gttagaattt ctttgagaaa
    24601 cgcaggaatt cctttaagtt tggcagaggt agaggtttat acttataagt aattttaaaa
    24661 attatcatcg gtagtatgaa agaaacagaa aaataagagg aaaatagtat gattcaacat
    24721 ccacgtattg ggattcgtcc gactattgat ggtcgtcgtc aaggtgtacg cgaatcactt
    24781 gaagtacaaa caatgaacat ggctaaaagt gtggcagatt tgatttcaag cacattgaaa
    24841 tatccagatg gggaacctgt ggaatgcgtg atttctccat ctaccattgg tcgtgtacca
    24901 gaggctgcag cttcccatga gttgttcaaa aaatcaaatg tttgcgcaac aattacagtt
    24961 acaccatgct ggtgttatgg tagtgaaact atggatatgt ctccagatat tcctcatgct
    25021 atttggggat ttaatgggac agaacgccca ggagctgtct atcttgcagc tgtactagct
    25081 tcatatgctc aaaaagggat tccagccttt gggatttatg gtagagacgt ccaggaagct
    25141 agtgatacag ctattccaga agatgtcaaa gaaaaacttt tacgttatgc gcgtgcagct
    25201 cttgcaactg gcttgatgag agacactgct tacctatcaa tgggtagtgt ttcgatgggg
    25261 attggcggtt ctattgtaaa ttcagatttc ttccaagaat acttaggaat gcgaaatgaa
    25321 tcggtagata tgacggagtt cacgcgccgt atggaccgtg gtatttacga ccctgaagag
    25381 tttgaacgtg ctatggtttg ggtaaaagaa catatcaaag aaggagttga ccgaaatcgt
    25441 gaggatttga ttctttctaa agaagagaaa gaaaaacaat gggaatttgt tattaagatg
    25501 ttcatgattg gacgtgactt aatggttggt aacccaagac ttgctgaact tggttttgaa
    25561 gaagaagcag ttggtcacca tgctttagta gctggtttcc aaggtcaacg ccagtggaca
    25621 gaccatttcc caaatgggga ctttatggaa actttcctca atactcagtt tgattggaat
    25681 ggtattcgaa aaccatttgt atttgcgaca gagaatgatt cactaaatgg tgtgtctatg
    25741 ctctttaatt atctattaac aaatactcca caaatctttg ctgatgtgcg tacttattgg
    25801 agtccagagg ctgttaaacg tgtaacggga catactttag agggttgtgc tgcagctggc
    25861 ttcttacatc taatcaactc tggttcttgt acattggatg gtacagggca agctactcga
    25921 gatggcaaac ctgttatgaa accattctgg gagttggaag aaagtgaagt gcaggctatg
    25981 cttgaaaata cagacttccc accagcaaac cgcgaatact tccgtggagg aggattctca
    26041 actcgtttct tgacgaaggg ggatatgcca gtaacaatgg tacgtctcaa tcttctaaaa
    26101 ggggttggtc cagtgctaca aattgcagaa ggttatacac ttgaacttcc tgaagatgtt
    26161 caccatactt tagataatcg tacagatcca ggatggccaa ctacttggtt tgctccacgt
    26221 ttgacaggaa aaggtgcttt caagtctgtc tatgacgtca tgaataattg gggagctaat
    26281 cacggagcca taacatatgg acacattgga gcagacttga ttaccttggc ttctatgttg
    26341 agaattcctg tcaatatgca taatgtaccc gaagaagata tctttagacc taaaaattgg
    26401 tccttatttg gaacagaaga tctagaatca gcagactatc gtgcatgtca gttattggga
    26461 ccactacata aataaaactt gtttatatag gaggtgaact tacgtccctc ctatcctttt
    26521 aaaaagattt gttaaacaat tcacaaataa ttgaaaacga atacaaaaag taatataatg
    26581 atgttaaata gatagcgcgg aggcgcagga ggaaaattat atggctacat tttatgttcc
    26641 ggcagtcaac cttattggaa aaggtgttgt aaatgaagtg ggtccttata tcaaggaact
    26701 tggctataaa aaggcacttt tggttacaga taagtacatc gaaggcagtg atattttacc
    26761 taaggtttta aaaccactgg atacagaagg aatcgaatat gtcatcttta gcgatgtaga
    26821 gccaaaccct acttgtaaaa atgtcacaga tggggtagct gctttgcaag aacatggctg
    26881 tgactttatc atcagtcttg gcgggggttc tccacaggat gcagctagtt gtatttctat
    26941 catggctaca aatggtggaa aaccacagga ttatgaaggg cttcataagt ctgctaaaaa
    27001 aggcttgcca gttgtggcta tcaatacaac ggcaggaact tctgcagaaa tcactattaa
    27061 ctatgtgatt actgatgaag aacgcaaggt taagatggta atggttgaca agaatagcct
    27121 tgctcttatc tctgttaatg acccggaact catgctttcc aaacctaaag gcctgactgc
    27181 tgctactggt atggatgctc tgactcacgc tgttgaagct ttggtaacac ctggtgctta
    27241 tgatgtaacc aagaaactgt ctattggtgc tattgagctt atcaaggaat atcttcctcg
    27301 tgctgtagaa aatggacatg atattgaagc gcgtgaaggt atggtcaatg ccatcttcct
    27361 tggtggtatg agctttaata atgctggtct tggttatgtt cactcaatgg ctcaccaact
    27421 cggtgcagta tataatttgc cacatggtgt ttgctgtgcc atgttgctac cagttataga
    27481 acgtgaaaat gctaaacgtg taccagaagc tttccgcaat gttgccaaag ccttgggact
    27541 tcatgtagaa ggtaaatcag atcaagaatg tgccgattat gcgattgttg agattgagaa
    27601 gctttctgag acagtaggta ttcctaagaa actgactgaa cttggtattg aagaaaaaga
    27661 tttcgacttt gaataccttt ctaagaatgc cttgattgat gcctgcgcac caggaaatcc
    27721 atttatgcca accttagaag aaacgattgc cttttataaa gagttatttt agcaagagat
    27781 tggacgacaa taggacaatt cacctctaat tattgacatt tcaggaaaaa tcgcagtaaa
    27841 aacggcacag ccttctttaa atgtgctata atactagaaa aatacttgtg gaggttccat
    27901 tatggcaata tttttcatga tttttctgat tgtttgtgtg ctcctattgg tgatagtcac
    27961 actgagtaca gtttatgtgg ttcgtcagca gtcggtggcg attattgaac gctttgggaa
    28021 ataccaaaag gttgccaata gcggtattca tattcgcttg ccttttggga ttgactcgat
    28081 tgcagcacgg attcagttgc gcttgttgca aagtgatatt gtggttgaga ctaagaccaa
    28141 ggacaatgtg ttcgttatga tgaatgtagc gactcagtac cgtgtcaacg agcagagcgt
    28201 gacagatgct tactataaac tcatacgtcc agaatctcag attaaatctt atatcgaaga
    28261 tgctcttcgc tcttctgttc caaaattaac cttggatgaa ttgtttgaga aaaaagatga
    28321 gattgccctt gaagttcaac accaagtagc agaagaaatg accacttacg gctacattat
    28381 cgtgaaaacc ttgattacca aggtcgaacc agatgcagaa gttaagcaat ccatgaatga
    28441 aatcaatgcg gcgcaacgta agcgggtcgc agcacaagaa ttggcggaag ctgacaagat
    28501 taaaattgtc actgcagctg aagcagaagc agaaaaagac cgccttcatg gtgtggggat
    28561 tgcccaacaa cgtaaggcga ttgtggatgg attggcagag tctatcaccg aactcaagga
    28621 agccaatgtt ggcatgacag aagaacaaat catgtctatc ctcttgacca accagtattt
    28681 ggataccttg aatacctttg cctctaaagg aaatcaaacc atctttttac caaatacgcc
    28741 aaatggtgtg gatgatatcc gaacacaaat cttgtcagcc cttcgcgctg agaagaaata
    28801 atagactaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt gccgtatatt
    28861 tgagctgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc aacctgtggc
    28921 tagtttccta gtttgctatt tgattttcat tgagtacaaa gagcttggca acaggctctt
    28981 tttgtattgc gtttattaag aaaaacaaaa aacatagata tacatttaac taaaaattat
    29041 tgtatgttac ctcccttaat taggaatgat aagggaataa ctagaaagat ttgtgaatac
    29101 aaactatttc tgatatacaa aatatacagt aataatgaat gatgggagat gggatgaaag
    29161 aatttcaatt tgagagaaag cagcgttttt ctttgaggaa atatgcaata ggagcttgtt
    29221 cggtcttgct aggaacgagt ttattttttg ctggtatggg tgatcagcct gtacaggata
    29281 cagaaacgag ttcagcacta atttcaagtc attatttgga tgagcaggat ttatctgaaa
    29341 agctgaaatc tgagttgcaa tggtttgaat tagaaaacaa gcttttgaac ttatgggagc
    29401 attaggttac tatgaaggat ttgttcctta tgtttcaaat caatacaaaa accaagctga
    29461 agaagaaggc aaaccgctat ctgataaata tattttcgaa aaaatcttag gaaaaacata
    29521 tgcagccttc aaaaaagatc aaattaatga gcgtgtcgag aaattaggta agttaaaacc
    29581 tattacaata aattacaacg gaaaatcaga agtaattgat agtaaagaaa aattacaaga
    29641 gcttatgaat aaagccgtta aagacgaagt ggctcaaata tagtaaaatg aaataagaat
    29701 aggacaaatc gttcaggaca gtcaaatcga tttctaaaaa tgttttagaa gtagaggtgt
    29761 actattctag tttcaatata ctataaaatc aggcaatacc acggctaaaa agtttgagtt
    29821 tatcgaaaca ccagtgcaaa aactgaaaaa atcgatttat aaagctcatc tcaaagattc
    29881 agatgacttt agaccagaaa catccacaca aatctttttg aaagctgttt gaagctttgt
    29941 ccttgctttc ttagttcata gatctgaact ttatcttcat aactcaattt cataataaaa
    30001 caccccaaaa gttagattta ttctgtctaa cttttggggt gcagttcatt aatcggtcga
    30061 ttttctttgg tagaaacagt tgcctgatta agttgcaagt cgatagacgg cttcggcata
    30121 aatatccata gcacggtaca aatcttctag aacggcacat tcatttgcct gatgttctgt
    30181 ctgcttcgct cctgggaata aggcgccgaa ggctacacaa tttggcatgg tgcgagcaaa
    30241 agtggcacca ccggatgaaa tagcaggact gttatcgcca gtcttttctt ggtagatttg
    30301 catcagtgtg ctaacgagtt tactgtcttt tgcgacgtat agaggcgcta gatagtcaaa
    30361 ctcttcgtag cggagttggt agttttgtgc acatcttgta agcaactcta ctagttcttc
    30421 cttgtcagct aagacaggag tccgaatgtc aatatggatt tcagaacgtt tatgattgat
    30481 catgagacct gcgacattaa aggataggtg accagaaggt tcatctgcta tatcaccaaa
    30541 gatttgtctt cctgtgccgt cttgacctgc ttgtgttgca agaaagctga gagcagggtg
    30601 ttcttggaga ggagcaagaa tggtagctag tcggatgaca gcattgatac cttgactagc
    30661 atccttagca tgctttggca ctccgagaac cgttacggtt tgttcagtgg tttggtaatc
    30721 ataaccagct tctttgagac cgttacaaac ctgttcatag aggggacctt ggtagttggc
    30781 cttgtctggt acaacgttaa aggcgcctcc tacttcaagc tctagttgat ccgatccagg
    30841 gccatgaagt ttgacctgta gaagcccttt ttcagcatag gtcagaggaa aagatgagtc
    30901 aggtgcaaag cccatgctgg cctgttcttc gatggtattg tagcgtgcca tgcagcgcca
    30961 gagggtttcc tcatcggtac caaagataaa gcgtacgcgc tttttgaact gaataccttg
    31021 gtccagcaag ctttttactg catagagagc tgcgagcgaa gggcctttat catcttggac
    31081 accacgtccg aatacccagc cgtctttgat agttgcttca aatggcggtg tctgccaatc
    31141 tgcttcatca cctgatggaa caacatccaa atgacagaga atggccagaa gctctgctcc
    31201 ctgaccgatt tctgcatatc cgtaataacc tttagggtca agataggtag tgaaacctat
    31261 gtctcgacaa atctctaaag ttttttctag gacatcttgg attgcttgtc caaaaggtgt
    31321 tccattttct ccttcattga gtactgaagg ataggaaatc aaggttttta atgatataag
    31381 aaattcatct ttaacttgat ttgttatatc tattttcatg gaatcacctc actcactatt
    31441 ttataggaat ggaaggaagg ttccaaggag aagaagggcg atggtcacta caataatagc
    31501 gactacgagt ttgcccatga atttccacca agtaccaatg ttgatacgtc caagtgcaag
    31561 agctcccatc acaataccag atgttggtgc aatcaagttc aagacacctg aagcagattg
    31621 gtaagcagtg ataatcaagc taggacggac atttacaaat tctccaagtg gagccatgat
    31681 acccatagtt gcgctggcaa gaccagatga agatgggatc aagaatgaca taggtagata
    31741 gaagatataa gttacaacga taaagacttg tgaagataga ccgctcaagc cttctttacc
    31801 ccagttgagg attgtatcgg taatcatacc gtcgttcatg ataacttgaa taccacgagc
    31861 aatcgctacg atcaaggcaa cactgagcaa gtcagcagca ccattcatga aggaagagat
    31921 aatcttatct tctttaagac cataaataac accaatcagg atacccataa aggcaaagag
    31981 cattgcgcct tctgggaagt accaagtacc tagtgcagaa gtagatgaac cgacaatatt
    32041 accaataact ggaagaccag tcaaccaagc attaaagtca tcaaaaatgg taacgccaag
    32101 gtctgtccat ggaatgaagc tcaataccat caagatgaat gtcaacacaa ataagaagag
    32161 aactgatttt tgtttgctgc taagtgtaga ttctacagat gaagattctt ctacgttaaa
    32221 gtgtttcaaa tcttctttgc gagtactata aaccagtgac ttagtcggat ctttttgaat
    32281 cttatccgca taacggtaaa caaaccaagt actaagagca gtcaaggtaa cccagaagat
    32341 cagacgaagt acgataccgt cccctgtacc aactcccgca gtcgctgaag caatacctgt
    32401 cgcaaatgga ttcagagtag atgccaaaca gccgatttga gaaccgagca aaataattgc
    32461 aacaccagtc aggctatcaa aaccaacggc catcataact ggcacaagga gtggatagaa
    32521 ggccattgtt tcttccccca taccataagt tgtaccaccg agggcaaaca aaggcatcag
    32581 taccaaaatt aacatttttt cgcggccctt atacttcttc acgatagagg caatccctac
    32641 gtcaagagca ccagttttgt tgacaatgcc aaggaaaccc ccaaccataa ggatgaagaa
    32701 ggctacatca atcgctgcgc tcgtttcttt aatgagcgaa ccttcctctg gatgagtacc
    32761 tagcatagcc cgaatcggtg ccatgaggac atcccaaatc ccttgtggat tttgaggctg
    32821 agtctcgtaa ataccttcta taaaggcccc cgcagggata aaccaagtta gcactgccat
    32881 aatagcaatg attatcaata atacggtgta agatgaaggc atcttaaacc cttttttagc
    32941 tttttcactc atttgttttt cctcctgatg tctaacaaag tgtaaaaaac caactacttt
    33001 cttaaataaa cattcaagaa aacgctttct atttatattt gttttaccat attaaagtct
    33061 aaaaaactaa tatcttttga aatattgtac atgttttcaa gaataatttc taatttgata
    33121 taaagatact agaaatagaa tacatcttat tagtaaaaca acttatcctt tttcaataat
    33181 tgttccgctt tcagattcaa tcaaggcgcc tagattttca agggaagtaa taactgcttt
    33241 tccttctgga cgaccattga caaaagcgat agctgcttct acttttggaa gcatgctacc
    33301 tggtgcaaac tgatcttgtt tgatatattc ttccagctgg gcaacattca catgttccaa
    33361 tttttcctgg tttggcttgt tgtagttaac aaatacataa tctacacctg tcaaaacgat
    33421 gaagaggtct gcatcaacta attctgccaa acgttgggaa gcgaagtctt tatcaataac
    33481 cgcttcgaca ccagtcaaat gtccattgtt ttctttgacg acgggaatac cgccaccacc
    33541 tgcagctacg acgacttgac cattatttaa aagagtacgg atggtttcaa tttctttgat
    33601 gtcaacaggt tttggtgagg caacgacctt acgccagcca cggccagcat cttccttgaa
    33661 agtcgctccg ctcttttcgg cttctgcttt tgcttcttct tctgaataga aaggaccgat
    33721 tggtttactc aagttaacaa aagccggatc atttttatct acgacaactt gcgttacaac
    33781 agaggcaaca tttttttcga tgccttcatc caagagagca ttttgcaaag catttttcaa
    33841 ccagaaaccg atgctacctt ctgtcatagc gacaagtgag tcgagtggga aggcagggtt
    33901 cttttcagag tctgatgcca aatgttggag caagagattc ccaacttgag gtccattacc
    33961 gtgagtgata atcaaatcat ctccattttt aatcaatttt acaagatgct tagctgtttc
    34021 aactaaagct tcttgttgag cctttgctga tgggtcagaa gaaagaatcg catttcctcc
    34081 caaagctact acaattttac gatttgccat aaattctcct ttatcacact caatagaatg
    34141 cgtttagatt tcaatttaat gatttttcac atattttata agaaataata gattaccatt
    34201 acataaaaga ggaccggact aaagctatta gtcgcagccc tcatagctgt tggtagacgg
    34261 tttattatct aaaattatac tttaggaata taaaggttac caagtgtagc agccataaca
    34321 gctttgatag tgtgcatacg gttttctgct tgatcgaagt ggcgagcgta cttgctgcgg
    34381 aagacttcgt ctgttacttc catttcttct acaccaaatt tttcagcaac gtctttacca
    34441 taaacagtgt gagtatcgtg gaatgctggc aagcagtgta ggaagatcaa gttttcattg
    34501 cctgcttttt taactaagtc catattgact tggtaaggtt taagaagagc tacacgttct
    34561 gcgaatttgt cttcttcacc cattgatacc caaacgtctg tgtaaagaac gtctgcatct
    34621 ttaactgctt catcagcatc ttcagtgatg agaacatgtg cgccactttc tttagcaaat
    34681 ccttctgcca attcaacgat ttctttttct gggaagagtt cttttggtga gaagatgtga
    34741 acattgacac caaggatagc acctgttacg agcaagctgt tggcaacgtt gttacgtcca
    34801 tcaccacagt ataccaatgt caagccttcc aagcgaccga agttttcttg aacagtcaag
    34861 tagtcagcga gcatttgagt tgggtgccat tcgtcagtta gaccgttcca tactggaacg
    34921 cctgagaatt ctgccaattc ttcaaccata cgttggctga atccgcggaa ttcaatcccg
    34981 tcaaacatac gtcccaatac tttagcagta tcttcagtag attctttttt acccaactga
    35041 atatcatttg ctccgaggta ttctgggtga gcaccaaggt cgatagccgc agttgtaaag
    35101 gctgcacgag tacgagtaga tgttttttca aataggagag cgatattctt gccagcaagg
    35161 tagtggtgtt gaatattgcg ttttttcaaa tctttcaagt gagctgaaag accaataagg
    35221 tattctaact ctgcacgggt aaagtctttt tctgctaaga agctgcgtcc ttggaatact
    35281 gaatttgtca ttttattatt tcctctttct attttttaca ttttctattg acgaatatcg
    35341 aacagcgatt acacttcttc acgttcaaat ggcatagaca tacaacgagg tccaccacgg
    35401 ccccgaacca attcacttcc gcgaatctta atcaagcgaa gcccgtattc ttccaaaatc
    35461 ttattggtca cggtattgcg gtcataaaca actaccacac caggtgcgat ggtcaaagtg
    35521 ttagaaccgt cgttccattg ttcacgcgca gctgctacga tattgccacc accgcaacga
    35581 atcaaatgaa ctttttctac accaaggttt tgagcaagaa gttcagctaa gtcacctttc
    35641 tcttcaacga ttttaagttt ttcgttttcg taagtaactg agtaaacgtg aaggtcgcct
    35701 tcgatttctg ggtgaatagt gaacttgtca tagtctacca tagtgaagac agtatccaag
    35761 tgcatgaatt tacggttgtt agcaaattca aaggccaaaa ctttcttgaa gccaacattt
    35821 ttcttgaaga tgttgaccaa aagtttttcg atagaagctg cgtctgtacg ttgagagata
    35881 cctactgcaa ggacgtcttt agaaagaact agctcgtctc caccttcgat acgcgtatct
    35941 tcttcacggt tgtagaccaa atccactttt ccgccataga ctgggtggta tttgaagata
    36001 tacttaccgt agagtgtttc acggttacga gtgtctgcaa acatgtggtt aagcgatacg
    36061 gcgtttccaa ttgttgcaaa tgggtcgcga gtgaaataga ggtttggcat cgggtcaatt
    36121 gcaaatggat aatctgattc aactaagtca gttagatctt tagcttcgtc aggaatttct
    36181 ggcaattcaa ctttttgaat cccagccatt gttttttcaa ccaattcttg gttgtccttg
    36241 atgccgtgaa gcaattcacg aatagcaacc ttggtttgac gatcacggat gttggcttcg
    36301 tctaagtatt cctcgataaa ttgatcgcgg atttctggag aggtcaatga ttcagcagcg
    36361 agttgttcta ggtatagaac ctcaattcct tcatcgcgaa gagcttgggc aaatgcatca
    36421 tgttcttttt gagcatcttc caagaaagga atatcatcaa aaagaagcct ttcaagatag
    36481 tccggcaaca agttttctaa ctccttgcct ggacggtgca acataacttt tttcagtttc
    36541 ccaatttctg agaagacctg aattggatgt gaagacatgt attttcctcc ttaaaaattt
    36601 tgacttggca ccattttggg taccgcttac atgtatgtta taacgcaatt cctaggtaat
    36661 gtcaatggct ataatttctc ggaaaataat ataaattatg atgtttagca caattttatg
    36721 ttattataca taacatagga tagaatacat agatacaaat gcataaactt ctataaaaag
    36781 gatataaaat ttttttaaaa aatttttcaa aaaatataca aatgaccaaa aagtgtccaa
    36841 ttatcatctg gttatcactt ggatctgtat aaagagaaat gtttgtgtga aaggataaaa
    36901 agatgcaaca ttcttttgaa gatagatttt ggagaagcat ttcttgttca attgtacaaa
    36961 ttatagaatc ttttctgagt atttaggaag agataattat cttattgacc ctctttttat
    37021 tttttgttat agtcgacgag gtgatagttt agaataatca cttattcaca atctgtggaa
    37081 aagtctggat agtgaatggt cttcacacaa tgacctgaaa gaagcctgag aataattatg
    37141 gagagtagca ttctgagagg tgttagcaga accatatgac agagctgttt gaagagggaa
    37201 tattgaggag aaaaatcctg agcctaccag ttggagttgg aaagagctgg cggttagatc
    37261 attttttatt atccacaacc tgtggataac cttgtgaaca agagaagttg ctaaagaagg
    37321 agatatataa cgatgaagaa aatcaaaccg catggaccgt taccaagtca gactcagcta
    37381 gcttatctgg gagatgaact agcagctttt atccacttcg gtcctaatac cttttatgac
    37441 caagaatggg ggactggaca ggaggatcct gagcgcttta acccgagtca gttggatgcg
    37501 cgtgagtggg ttcgtgtgct caaggaaacg ggcttcaaaa agttgatttt ggtggtcaag
    37561 caccacgatg gctttgtcct ttatccgaca gctcacacag attattcggt taaggtcagt
    37621 ccttggagga gaggaaaggg cgacttgctc cttgaagtat cccaagctgc cacagagttt
    37681 gatatggata tgggggtcta cctgtcaccg tgggatgccc atagtcccct ctatcatgtg
    37741 gaccgagaag cggactacaa tgcctattat ctggctcagt tgaaggaaat cttatcaaat
    37801 cctaactatg ggaatgctgg taagttcgct gaggtttgga tggatggtgc cagaggagag
    37861 ggcgcgcaaa aggttaatta tgaatttgaa aaatggtttg aaaccattcg tgacttgcag
    37921 ggcgattgtt tgattttttc aacagaaggc accagtatcc gatggattgg caatgaacga
    37981 gggtatgcag gtgatccact gtggcaaaag gtgaatcctg ataaactagg aacagacgca
    38041 gagctgaact atcttcagca cggggatccc tcgggcacga ttttttcaat cggagaggca
    38101 gatgtttcca tccgtccagg ctggttctac catgaggatc aggatcctaa gtctctcgag
    38161 gagttggtcg aaatctactt tcactcagta gggcgaggaa ctccactctt gcttaatatt
    38221 ccgccgaatc aagctgggct ctttgatgca aaggatattg aacgacttta tgaatttgcg
    38281 acctatcgca atgagctcta taaagaagat ttggctctgg gagttgaggt atctggtcca
    38341 gctctttccg cagactttgc ttgtcgccat ttgacagacg gccttgagac cagctcttgg
    38401 gcaaacgatg cagacttgcc catccagtta gaactcgact taggttttcc taaaactttt
    38461 gatgtaattg agttaagaga agatttgaag ctagggcaac gaatcgctgc ttttcatgtg
    38521 caagtagagg tggatggtgt ctggcaggag tttggttcgg gtcatactgt tggttacaaa
    38581 cgtctcttac gaggagcagt tgttgaagca cagaagatac gtgtagtcat tacagaatca
    38641 caggctttgc ctttgttgac caagatttca ctctataaaa ctcctaggtt atcacaaaca
    38701 gtagctgttc aggggctagc atttgcagaa aaaagcctag ctgtggcaaa gggagagact
    38761 cttcatttta ggattgaacg tagtgaaagt agctcctctt tagagactaa gatttcgatt
    38821 caaccgggga caggtgtcca tggtgtcgcc tatcaggatg agattcaagt ccttcaattc
    38881 caagcagggg aaagcaaaaa agatttgcat ctaccaaccc tgtatttcgc aggagataaa
    38941 accttggatt tctatctgaa cctaacggtg gatggtcagc ttgtggatca acttcaagtc
    39001 caagtttcat aaaagaagaa cctttgcgcg atgcaaaggt tcttttggtt attagtgact
    39061 tggtaaccag ctgagggtga aagttagttg ttcagctttt aagaggtctt ggtgttgaat
    39121 agttgatacg agtgttttgt ccagtcggca ttctttgaca aagttaaaat ggttgtggtt
    39181 ttgtttagta tggatatcca gccatttatc ttctttagcg aggtagactc gtagatggtc
    39241 aaagagaggg attccgaggt catagcttgg ttttcctgga caggttggat aaaatccgag
    39301 agctgaccag atgtaccaag cagagagact accattgtct tcatctccag gataggcttc
    39361 ccaacttggg tgaaaagctt tctgacggag cgtcttgata agaagggcag tgtagtcagg
    39421 gtaatcgctg tagcggaaga gataaggaat gtggaaacta ggctggttgg aaatggctat
    39481 ttgtccaaaa ggagcagtag ccatctcgct catttcgtga atttcgtaac catagcctgt
    39541 tgtttcaaag aggggagcat cttgacaggc tttcaaaaga tagttgctaa aggtttcttt
    39601 tccacccatc agttggatta agccagggat gtcgtggaga acgcctaaag tagcttgaat
    39661 ggcagagcat tcagcgtagt ctcgccccca actataaaga gagaagtcag ggtgaaagtt
    39721 tccttgattg tctcgtgctc gcatgtaacc tgtctcagcg tcaaatagct ggcggtaatt
    39781 ttgtgaagca gccttgtagg tttcagcgat ttctatgttt tttagttttt tggcacagtt
    39841 ggcgatacaa aagtcactat aggcatagtc tagagtatgg ctaacacttt cgtggtggtc
    39901 ggtagagagg taacctagtt cttggtattg ggctagtccg tggcggccat tgatgccgag
    39961 agggtcggct ttgctggctg ttttgagcat ggcttggaag agttctcctt ctaggtcggg
    40021 ggccatgtcc ttgcaggcgc tatctgcgat aataccgtct aaaagtgtac ctggcatcat
    40081 accccgttca tctggagcca gccattttgg aaggaaacca gtatcgcggt agctattgag
    40141 gaaaccttct aaaaagcgtt gatagtgctc cggtatgata agggcaaaga gggggaaggt
    40201 ggtgcggaag gtatcccaga aaccattgtt gctaaagagg acaccaggct tgacagtacc
    40261 agtagccaga tccatgtgga tggcttgccc tgactcatca acctcataaa aagtctgtgg
    40321 gaagaggaag agtctgtaga gacagtggtc aaagaaggtt cggttagcct ctcctgtctc
    40381 tataacatca aaacgatgga ggagattttc ccaatccgct tgggcacttg ctttacagct
    40441 atcaaaatct tcttgaggta gattgattag agcttgagaa ggagagatga aagaagtggc
    40501 tagttgcatc tcggtttgac tacttactaa gtcaattcgc cagtctccag cttcttggct
    40561 gatagcaaga atatccgtgt tcatttgtag ggcagtgaac atcgttagcg aatttttgtt
    40621 ggtttcagtt ttaccttctt gtcgcagggc aagagttcgc ttatctactt gctctactgt
    40681 cagttcatct gctgcgtgaa gatagaggga gagggctttg ccttgctttt gcttcaaacg
    40741 aatagaagca ccgtagcaag tcggtgtgag ctgggtttca atctgatagc gcagggagaa
    40801 aatctttaga taatgaggtt ggaaagaggc cttatcccta tcataggaag attggcggtg
    40861 aaagagactg tctcccccaa gctgacctgt gacaggtgtc agaaggagcc aagagtagtc
    40921 cccaatccaa ggactgggct ggtgagttaa tcgaatcccc tgaaagatag gcagatgcgg
    40981 atcaaaaaac caagatccat cctggtcact ggtctggggc acaaagtaat tcatcccaaa
    41041 aggcacgcct gtgtatggca gggtatttcc ccgagaaaag gcatgcttgt tggtagttcc
    41101 aaagcgggta tcgatggttt caagtagtgg tttcatagtc tttcctttag ctgtttttct
    41161 acattatatc agaaaaagag ggcctttaga aaaggagttt caaaaataac tattttgtag
    41221 aaaaatgact atcatttgtg tatgtatttt ctgtctcaaa agctatatac tgaacatgaa
    41281 acttgtttga aagagggaat tgcacgatga tttattcgaa agaaattgtt agagaatggc
    41341 tagatgaagt agcagagcgg gctaaggacc atccagagtg ggtggatgtt ttcgagcgtt
    41401 gctacaccga taccttggac aatacggttg aaatcttaga agatggttca acttttgtct
    41461 tgactgggga tattcctgcc atgtggcttc gagattcgac agcccaactc agaccctacc
    41521 ttcatgtagc taaaagagat gccctcctgc gtcagaccat tgcaggtttg gtcaaacgtc
    41581 agatgacctt ggtactcaag gatccctatg ctaactcctt caacattgag aagaactgga
    41641 aagggcacca cgagactgac cacacagacc ttaacggctg gatctgggag cgcaagtatg
    41701 aggtggattc actttgctat cctttgcagt tggcttatct cctctggaaa gagactggcg
    41761 agactggtca gtttgatgag acttttgtcg cagcgactaa ggaaattctc catctatgga
    41821 cagttgaaca agaccacaag aactctcctt atcgttttgt ccgagatacg gaccgtaagg
    41881 aagacacctt ggtaaatgat ggctttggac ctgactttgc agtgacaggt atgacttggt
    41941 cagcctttcg tccgagtgat gactgttgcc agtatagtta cttgattccg tcaaatatgt
    42001 ttgctgtagt agtcttgggt tatgtgcaag aaatcttcgc agcattaaac ctagctgata
    42061 gccagagtgt tattgctgat gccaagcgtc ttcaggatga aatccaagaa ggaatcgaaa
    42121 actacgctta caccaccaac agcaagggcg aaaagattta cgcttttgaa gtggatggcc
    42181 taggaaatgc cagcatcatg gatgatccaa atgtaccaag tctactagct gcgccctatc
    42241 tgggctactg ttcggtcgat gatgaagtgt atcaagctac tcgtcgtacc attttgagcc
    42301 ctgaaaatcc atatttctac caaggagaat acgctagcgg tctcggaagt tctcatacct
    42361 tctatcgcta tatctggcca atcgcccttt ctatccaagg attgacaaca agagataagg
    42421 cagagaaaaa attcttgctg gatcagctgg ttgcctgcga tggtggtaca ggtgtcatgc
    42481 acgaaagctt tcatgtagat gatccgaccc tctactctcg tgaatggttc tcctgggcta
    42541 acatgatgtt ctgtgagttg gtcttggatt acttggatat tcgctaaggg gctcgcttta
    42601 gctcaaccga ttcttatcag aatcacaagt ttacatttaa aacgttaaaa tttaaattta
    42661 gaatgaggtt ttacttcatg gaaaatgttg ttgtacatat tatctcacat agtcactggg
    42721 accgtgagtg gtacttgcct tttgaaagcc atcgtatgca gttggtggaa ttgtttgaca
    42781 atctctttga tctctttgaa aatgaccctg agttcaagag tttccacttg gatggacaaa
    42841 ctattgtcct tgacgactac ttacaaattc gccctgaaaa tcgtgacaag gtccaacgct
    42901 acattgacga gggcaaactt aaaattggtc ccttttacat cttgcaggat gactacttga
    42961 tctccagtga agccaatgtc cgcaatacct tgattggtca acaagaagct gccaaatggg
    43021 gtaaatcaac ccagattggc tactttccag atacctttgg aaatatggga caagcgcctc
    43081 aaattcttca aaaatcaggc attcacgtgg cggcctttgg tcgtggtgtg aagccgattg
    43141 gatttgacaa ccaagtcctt gaagatgagc agtttacatc ccagttttca gaaatgtact
    43201 ggcagggtgt ggatggtagt cgtgttttag gtattctctt tgccaactgg tacagtaatg
    43261 ggaatgaaat cccagttgac aaagatgagg ccttgacctt ctggaaacaa aaattgtcag
    43321 atgtgcgtgc ctacgcttcg accaaccaat ggttgatgat gaacggctgt gaccaccagc
    43381 ctgtacagaa aaatctgagc gaagccattc gtgtggcaaa tgaactcttc ccggatgtaa
    43441 tctttgttca tagttctttt gatgaatatg ttcaagctgt agaaggtgcg cttcctgaac
    43501 acttatcaac tgttacaggc gagttgacca gtcaggaaac agatggctgg tacacacttg
    43561 ccaacacttc ttcatcccgc atttacctaa aacaagcctt ccaagaaaat agcaacctcc
    43621 tagagcaagt ggtagaaccc ttgactatta tcactggtgg acacaaccac aaggaccagt
    43681 tgacctatgc ttggaaaaca cttttgcaga atgcgccaca tgatagtatc tgtggctgta
    43741 gcgtggacga agttcaccgc gagatggaaa cgcgttttgc caaggtcaac caagtaggaa
    43801 actttgttaa aagtaatttg ctcaacgagt ggaagggtaa aattgctacg gataaggctc
    43861 aaagtgacta tctctttact gtcattaaca caggcttgca tgataaggtc gatactgtct
    43921 gcacagtgat tgatgtggcg acttgtgatt tcaaggaatt gcacccaaca gaaggctaca
    43981 aaaagatggc tgctcttatc ttgccaagtt accgtgtgga ggacttggat ggtcgtcctg
    44041 tagaggttac aatcgaagac ctcggagcta attttgaata cgatttacca aaagataagt
    44101 tccgccaagc tcgtattgct cgacaagtgc gcgtgaccat tccagttcac ctagcaccgc
    44161 tttcttggac aaccttccaa ttgctggaag gagaacaaga acaccgtgag ggtatttacc
    44221 aaaacggagt gattgataca ccattcgtaa cggtgagtgt ggatgacaac atcacagtct
    44281 atgacaagac aactcacgaa gcctatgaag actttatccg cttcgaagac cggggtgaca
    44341 tcggaaacga gtatatctat ttccaaccaa aaggaacaga gccaatcttt gcagagctta
    44401 agggccacga ggtcttggaa aacacagatc gctatgctaa aatcttgctc aaacatgaat
    44461 tgaccgtgcc tgtcagtgcg gatgaaaagc tagaagaaga gcaacaaggt atcatcgagt
    44521 ttatgaagcg tgaggctgga cggtcagaag aattgacaaa cattcctctg gaaactgagt
    44581 tgattgtctt tgttgacaat ccacaaatcc gcttcaagac tcgctttact aacactgcca
    44641 aggatcaccg tatccgtctc ttggtcaaga ctcataacac gcgtccaagc aatgattctg
    44701 aaagtatcta tgaggtggtg acacgaccaa acaaaccagc tgcttcatgg gaaaatcctg
    44761 aaaatcctca acaccaacaa gcttttgtca gtctgtatga cgatgaaaaa ggggtgactg
    44821 tatccaacaa gggattgaat gaatacgaaa tccttgggga taacaccatt gccgtgacca
    44881 ttttgcgtgc atcaggtgag ctaggtgact ggggctactt cccaacgcca gaagcacaat
    44941 gcttgcgtga gtttgaagtc gaatttgcga ttgaatgcca ccaagcccaa gaacgcttct
    45001 cagcctatcg tcgtgccaaa gccttgcaga caccgtttac cagccttcag cttgctagac
    45061 aggaaggaag cgtggttgcg actggtagcc tcttgagcca ttctgttctc agcataccgc
    45121 aagtttgtcc aacagccttt aaagtggcgg aaaatgaaga aggctatgtc ctccgttact
    45181 ataatatgag tcaagaaaat gtgcgcatat cagaacatca acaaaccatt cttgacttac
    45241 ttgagcgacc atatccagtt cattcaggac tattggctcc acaagagatt cgtacagaat
    45301 tcatcaaaaa agaagaaatt taatttcaaa aagtaaacat caaaagaaag gaggggcgaa
    45361 aaagtaagaa ctaactgctg attcgcccct ttttatggta aaaacaatga ccattgcaac
    45421 gattgatatc ggagggactg ggattaagtt tgccagtctg actcctgatg ggaaaatact
    45481 ggataagaca agtatttcaa cgcctgaaaa cttggaggat ttactagcgt ggctagatca
    45541 acgcttgtca gaacaggatt acagtgggat tgctatgagt gttccaggtg cagtcaatca
    45601 agagacaggt gtgattgatg gcttcagtgc ggtgccctac atccatggct tttcttggta
    45661 tgaggcgctt agctcttatc agctacctgt ccatttagaa aatgatgcca actgcgttgg
    45721 actcagtgaa ctactagctc atccagagct tgaaaatgca gcctgtgtcg tgattgggac
    45781 agggattggc ggagccatga ttatcaatgg tagacttcat cgaggtcgcc acggtctggg
    45841 tggagaattt ggctacatga caacccttgc ccctgctgaa aaacttaata actggtcgca
    45901 actagcatca actgggaata tggtacgata cgtgattgaa aaatctggtc atactgattg
    45961 ggacggtcgc aagatttacc aagaggccgc agctggtaat gctctttgtc aagaagccat
    46021 tgagcgcatg aaccgcaatt tgacgcaagg cttgctcaat atccagtatc tcatcgatcc
    46081 agatgtcatc agtctgggtg gctctatcag tcaaaatcca gattttatcc aaggtgttaa
    46141 gaaggctgtt gaagactttg tcgatgccta cgaagaatac acggtcgcac cagttatcca
    46201 ggcctgcacc tatcacgcag atgccaatct ctacggtgct cttgtcaact ggctacagga
    46261 ggaaaagcag tggtaagatt tataggactt agtcccaaac aaacgcaagc tattgaggtt
    46321 ttaaaaggtc acatttctct accagatgtg gaagtggctg tcactcagtc tgaccaagca
    46381 tctatctcta tccagggtga ggaaggtcac tatcaattga cctatcgcaa acctcaccaa
    46441 ctttatcgtg ccttgtcctt gttggtaaca gttctagcag aagctgataa agtagagatt
    46501 gaggaacaag cagcttacga agatttggct tacatggttg actgttctcg aaatgcggtg
    46561 ctgaatgtgg cttctgccaa gcagatgatt gaggtcttgg ctctcatggg ctactcaacc
    46621 tttgagcttt acatggaaga cacttaccag attgaaggac agccttactt tggctatttc
    46681 cgtggagctt attcagcaga ggagttgcag gaaatcgaag cctatgctca acagtttgac
    46741 atgacctttg taccatgcat ccagaccttg gcccacttgt cggcctttgt caaatggggt
    46801 gtcaaggaag tgcaggagct ccgtgatgta gaggacattc ttctcattgg cgaagaaaag
    46861 gtttatgacc tgattgatgg catgtttgcc acgttgtcta aactgaagac tcgcaaggtc
    46921 aatatcggga tggacgaagc ccacttggtt ggtttgggac gctacctgat tttgaacggt
    46981 gttgtggatc gtagtctcct catgtgccaa cacttggagc gcgtgctgga tattgctgac
    47041 aaatatggtt tccactgcca gatgtggagt gatatgttct tcaaactcat gtcagcggat
    47101 ggccagtacg accgtgatgt ggaaattcca gaggaaactc gtgtctacct agaccgtctc
    47161 aaagaccgtg tgactctggt ttactgggat tattatcagg atagcgagga aaaatacaac
    47221 cgtaatttcc gcaatcatca caagattagc catgaccttg catttgcagg gggagcttgg
    47281 aagtggattg gctttacacc tcacaaccat tttagccgtc tagtggctat cgaggctaat
    47341 aaagcctgcc gtgccaatca gattaaagaa gtcatcgtaa cgggttgggg agacaatggg
    47401 ggtgaaactg cccagttctc tatcctacca agcttgcaaa tctgggcaga actcagctat
    47461 cgccatgacc tagatagttt gtctgcgcac ttcaagacca atactggtct aacggttgag
    47521 gattttatgc agattgacct tgccaacctc ttaccagacc taccaggcaa tctcagtggc
    47581 atcaatccca accgatatgt cttttatcag gatgttctct gtccgatact tgaccgacac
    47641 atgacacctg aacaggacaa accgcacttc gctcaggctg ctgagacgct tgctaacatt
    47701 aaagaaaaag ctggaaacta tgcttatctc tttgaaactc aggcccagtt gaatgctatt
    47761 ttaagtagca aagtagatgt gggacgacgc attcgtcagg cctaccaagc ggatgataaa
    47821 gaaagtttac aacaaatcgc cagacaagaa ttaccagaac ttagaagcca aattgaagac
    47881 ttccatgccc tctttagtca ccaatggctg aaagaaaaca aggtctttgg tttggataca
    47941 gttgacatcc gtatgggcgg actcttgcaa cgcatcaaac gagcagaaag ccgtatcgag
    48001 gtttatctgg ctggccagct tgaccgcatc gacgagctgg aagttgaaat cctaccattt
    48061 actgacttct acgcagacaa ggatttcgca gcaactacag ctaaccagtg gcataccatt
    48121 gcgacagctt cgacgattta tacgacttaa tactcttcga aaatctcttc aaaccacgtc
    48181 agcttccatc tacaacctca aaacagtgtt ttgagcagcc tgcagctagc ttcctagttt
    48241 gctctttgat tttcattgag tataaaaaca agaacacctt gcttggcgca gggtgtttcg
    48301 cgtgaaacag aagaattatc tggtttcaaa tgctacagtt aaaattagta aaacaagaaa
    48361 tagaagctga aaagccagca tctgtagagg cttggatcat ttccgtcaaa tttaaaaaag
    48421 gttgctaccg acatatatag attccaaaaa caaaaacgtt agcggaacta gcagatgtga
    48481 ttttatggag ttttgatttt gcaaatgatc atgctcacgc atttttcatg gataatattg
    48541 agtggagtca tgcagattct tactttctta gctttgttag tgacgatgtt gaagaacgtt
    48601 acacagaaaa tgtctatctg gatagcctaa gtgtcaaaca aaaatttaag tttattttcg
    48661 acttcggtga tgaatggcgt tttgaatgcc aagtgctgag agaaatcgag acagaggacg
    48721 aagaagctta tctcgtacgt tcggttggaa cgtcgccaga acaatatcca gattatgatg
    48781 gttttgacta tgaagaatgg taaaattgaa atcagtctgt gtaggcttag tatttcaata
    48841 gacttcctgc aaaactagaa tcctagttca tgattgataa taccagcaat caaattcatt
    48901 cgtaatccga agcgtttacg atgatttcga taggttgttg aaaacatttt aaacgttttt
    48961 actttggcaa agatgttctc aaccttgctt ctctccttgg atagcgcatg gttataggct
    49021 ttatcttcag ctgttagtgg cttgagtttg ctggatttac gtgaagtttg tgcttgagga
    49081 catatcttca tgagcccttg ataaccactg tcagccaaga ttttaccaac ttgtctgata
    49141 tttctgcaac tcattttgaa caacttcata tcatgacaat agttcacagt gatatccaaa
    49201 gaaacaattc tcccttgact tgtgacaatc gcttgagcct tcatagcgtg aaatttcttt
    49261 ttaccagaat gattcgctaa ttgttttttt agggcgattg atttgtactt ccgtcgcatc
    49321 aatcattacc gtgtcctcag agctgagagg agttcttgaa atcgtaacac cactttgaac
    49381 aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgga taccaaaatc
    49441 agccgcaatt tcttcataag tgcggtattc tcacacatat tgaagagtgg ccataagaag
    49501 gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt gataagctgt
    49561 ttttaataca gctaacatct ctttaaaagt cgtgcgctga acaccaacaa gacgcttaaa
    49621 tcgtgtatca gttaattgtt tacttgcttc ataatttcgc agggagtcta ttgactcttt
    49681 ggtaggtgtc aatgtttttt tcatctatcc cgagaattat tttcccgcca tttgtatttg
    49741 caaatgctga gtaggtttcc cagaaagact ctggaagatt gtttttagct tttttgtatt
    49801 ctaaatcaac cccttcaaat tttaagtcca tatttttcct ttacatctgt tttttgtggt
    49861 tctggtattt gttcaagttg agtgataata tagcgaattg aatttcgaga gtttttactc
    49921 agttaatttc ttttttaacc cactttaatt tcttttttaa cacgggttaa aaaagaaatt
    49981 aaagtgggtt aattttcctt gacttaaaat ttaaaaatga tatactgtgt ttatggagag
    50041 atgacaattt ataaaatgta ctactctatt ctactagata atgattgaat aaaacttttt
    50101 ataacaacgg ctagcaaagt tataatttta tatctattag aaaataaata tgtaaggaaa
    50161 tttaaatgaa aaaaactaca atattatcat taactacagc tgcggttatt ttagcagcat
    50221 atgtccctaa tgaaccaatc ctagcagata ctcctagttc ggaagtaatc aaagagacta
    50281 aagttggaag tattattcaa caaaataata tcaaatataa ggttctaact gtagaaggta
    50341 acataggaac tgttcaagtg ggtaatggag ttactcctgt agagtttgaa gctggtcaag
    50401 atggaaaacc attcacgatt cctacaaaaa tcacagtagg tgataaagta tttaccgtta
    50461 ctgaagtagc tagtcaagct tttagttatt atccagatga aacaggtaga attgtctact
    50521 atcctagctc tattactatc ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg
    50581 gaagtaaagc taaaactatt atttttgaca aaggcagtca gctggagaaa attgaagata
    50641 gagcttttga tttttctgaa ttagaagaga ttgaattgcc tgcatctcta gaatatattg
    50701 gaacaagtgc attttctttt agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa
    50761 aattagaatt aatatcacat gaggcttttg ctaatttatc aaatttagag aaactaacat
    50821 taccaaaatc ggttaaaaca ttaggaagta atctatttag actcactact agcttaaaac
    50881 atgttgatgt tgaagaagga aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa
    50941 aagataaaac tcaattaatt tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc
    51001 ctaaagagac aaaagaactt gcatcatatt cgtttaataa aaattcttac ttgaaaaaac
    51061 tcgaattgaa tgaaggttta gaaaaaatcg gtacttttgc atttgcggat gcgattaaac
    51121 ttgaagaaat tagcttacca aatagtttag aaactattga acgtttagcc ttttacggta
    51181 atttagaatt aaaagaactt atattaccag ataatgttaa aaattttggt aaacacgtta
    51241 tgaacggttt accaaaatta aaaagtttaa caattggtaa taatatcaac tcattgccgt
    51301 ccttcttcct aagtggcgtc ttagattcat taaaggaaat tcatattaag aataaaagta
    51361 cagagttttc tgtgaaaaaa gatacatttg caattcctga aactgttaag ttctatgtaa
    51421 catcagaaca tataaaagat gttcttaaat caaatttatc tactagtaat gatatcattg
    51481 ttgaaaaagt agataatata aaacaagaaa ctgatgtagc taaacctaaa aagaattcta
    51541 atcggggagt ag
//
LOCUS       DAVMMR010000005        50599 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMMR010000005 DAVMMR010000000
VERSION     DAVMMR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.005"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            253..465
                     /locus_tag="VYS74_000268"
     CDS             253..465
                     /locus_tag="VYS74_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788360.1"
                     /translation="MDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELI
                     KMRFNSSAIDPIYGKKDDASILVIDV"
     gene            523..1164
                     /locus_tag="VYS74_000269"
     CDS             523..1164
                     /locus_tag="VYS74_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV3788361.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERIELI
                     KEDLIINESVLNIDKKGYELICLQYPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QILQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSI
                     NMEKKIMNYIFEDDGNVSEVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            1177..2652
                     /gene="galT"
                     /locus_tag="VYS74_000270"
     CDS             1177..2652
                     /gene="galT"
                     /locus_tag="VYS74_000270"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV3788362.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEENAIADYHLEWADQLKEKYPRINKEEVN
                     NVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            2665..3675
                     /gene="galE"
                     /locus_tag="VYS74_000271"
     CDS             2665..3675
                     /gene="galE"
                     /locus_tag="VYS74_000271"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3788363.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            3855..4514
                     /locus_tag="VYS74_000272"
     CDS             3855..4514
                     /locus_tag="VYS74_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV3788364.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            4539..5606
                     /locus_tag="VYS74_000273"
     CDS             4539..5606
                     /locus_tag="VYS74_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3788365.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            5710..6720
                     /locus_tag="VYS74_000274"
     CDS             5710..6720
                     /locus_tag="VYS74_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788366.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6732..8423
                     /locus_tag="VYS74_000275"
     CDS             6732..8423
                     /locus_tag="VYS74_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3788367.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            8426..9136
                     /locus_tag="VYS74_000276"
     CDS             8426..9136
                     /locus_tag="VYS74_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV3788368.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            9232..9600
                     /locus_tag="VYS74_000277"
     CDS             9232..9600
                     /locus_tag="VYS74_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788369.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            9708..10709
                     /locus_tag="VYS74_000278"
     CDS             9708..10709
                     /locus_tag="VYS74_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3788370.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            11850..13211
                     /gene="trpE"
                     /locus_tag="VYS74_000279"
     CDS             11850..13211
                     /gene="trpE"
                     /locus_tag="VYS74_000279"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV3788371.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            13208..13774
                     /locus_tag="VYS74_000280"
     CDS             13208..13774
                     /locus_tag="VYS74_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV3788372.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            13785..14789
                     /gene="trpD"
                     /locus_tag="VYS74_000281"
     CDS             13785..14789
                     /gene="trpD"
                     /locus_tag="VYS74_000281"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV3788373.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            14786..15553
                     /gene="trpC"
                     /locus_tag="VYS74_000282"
     CDS             14786..15553
                     /gene="trpC"
                     /locus_tag="VYS74_000282"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV3788374.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            15540..16139
                     /locus_tag="VYS74_000283"
     CDS             15540..16139
                     /locus_tag="VYS74_000283"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV3788375.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            16117..17340
                     /gene="trpB"
                     /locus_tag="VYS74_000284"
     CDS             16117..17340
                     /gene="trpB"
                     /locus_tag="VYS74_000284"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV3788376.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            17333..18109
                     /locus_tag="VYS74_000285"
     CDS             17333..18109
                     /locus_tag="VYS74_000285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV3788377.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIAGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(18081..18182)
                     /locus_tag="VYS74_000286"
                     /pseudo
     CDS             complement(18081..18182)
                     /locus_tag="VYS74_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            18411..19142
                     /locus_tag="VYS74_000287"
     CDS             18411..19142
                     /locus_tag="VYS74_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788378.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            19157..20308
                     /locus_tag="VYS74_000288"
     CDS             19157..20308
                     /locus_tag="VYS74_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV3788379.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRASNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSICLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(20360..21019)
                     /locus_tag="VYS74_000289"
     CDS             complement(20360..21019)
                     /locus_tag="VYS74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV3788380.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            21089..21535
                     /locus_tag="VYS74_000290"
     CDS             21089..21535
                     /locus_tag="VYS74_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788381.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            complement(21763..22941)
                     /locus_tag="VYS74_000291"
     CDS             complement(21763..22941)
                     /locus_tag="VYS74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV3788382.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            23221..24483
                     /locus_tag="VYS74_000292"
     CDS             23221..24483
                     /locus_tag="VYS74_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3788383.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            24486..25382
                     /locus_tag="VYS74_000293"
     CDS             24486..25382
                     /locus_tag="VYS74_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV3788384.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            25397..25714
                     /locus_tag="VYS74_000294"
     CDS             25397..25714
                     /locus_tag="VYS74_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3788385.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            25731..26048
                     /locus_tag="VYS74_000295"
     CDS             25731..26048
                     /locus_tag="VYS74_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3788386.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            26058..26660
                     /locus_tag="VYS74_000296"
     CDS             26058..26660
                     /locus_tag="VYS74_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3788387.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSTGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            26566..27192
                     /locus_tag="VYS74_000297"
                     /pseudo
     CDS             26566..27192
                     /locus_tag="VYS74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            27207..27977
                     /locus_tag="VYS74_000298"
     CDS             27207..27977
                     /locus_tag="VYS74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV3788388.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(28255..28542)
                     /locus_tag="VYS74_000299"
     CDS             complement(28255..28542)
                     /locus_tag="VYS74_000299"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3788389.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            28851..29123
                     /locus_tag="VYS74_000300"
                     /pseudo
     CDS             28851..29123
                     /locus_tag="VYS74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(29234..29437)
                     /locus_tag="VYS74_000301"
     CDS             complement(29234..29437)
                     /locus_tag="VYS74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3788390.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(29468..30076)
                     /locus_tag="VYS74_000302"
     CDS             complement(29468..30076)
                     /locus_tag="VYS74_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3788391.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(30115..30285)
                     /locus_tag="VYS74_000303"
     CDS             complement(30115..30285)
                     /locus_tag="VYS74_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV3788392.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(30297..30863)
                     /gene="amaP"
                     /locus_tag="VYS74_000304"
     CDS             complement(30297..30863)
                     /gene="amaP"
                     /locus_tag="VYS74_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV3788393.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(30947..31111)
                     /locus_tag="VYS74_000305"
     CDS             complement(30947..31111)
                     /locus_tag="VYS74_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3788394.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            31554..33035
                     /locus_tag="VYS74_000306"
     CDS             31554..33035
                     /locus_tag="VYS74_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3788395.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            33397..34398
                     /locus_tag="VYS74_000307"
     CDS             33397..34398
                     /locus_tag="VYS74_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3788396.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            34488..35405
                     /locus_tag="VYS74_000308"
     CDS             34488..35405
                     /locus_tag="VYS74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3788397.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            35416..36306
                     /locus_tag="VYS74_000309"
     CDS             35416..36306
                     /locus_tag="VYS74_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3788398.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            36335..37951
                     /locus_tag="VYS74_000310"
     CDS             36335..37951
                     /locus_tag="VYS74_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3788399.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            37961..39280
                     /locus_tag="VYS74_000311"
     CDS             37961..39280
                     /locus_tag="VYS74_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3788400.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(39490..39562)
                     /locus_tag="VYS74_000312"
     tRNA            complement(39490..39562)
                     /locus_tag="VYS74_000312"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39527..39529),aa:Lys,seq:ctt)
     gene            complement(39826..41097)
                     /locus_tag="VYS74_000313"
     CDS             complement(39826..41097)
                     /locus_tag="VYS74_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV3788401.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            41367..41605
                     /locus_tag="VYS74_000314"
                     /pseudo
     CDS             41367..41605
                     /locus_tag="VYS74_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41770..41949
                     /locus_tag="VYS74_000315"
     CDS             41770..41949
                     /locus_tag="VYS74_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788402.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            42092..42265
                     /locus_tag="VYS74_000316"
     CDS             42092..42265
                     /locus_tag="VYS74_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV3788403.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            42302..42754
                     /locus_tag="VYS74_000317"
     CDS             42302..42754
                     /locus_tag="VYS74_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV3788404.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            43045..43515
                     /locus_tag="VYS74_000318"
     CDS             43045..43515
                     /locus_tag="VYS74_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV3788405.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            43534..44601
                     /locus_tag="VYS74_000319"
     CDS             43534..44601
                     /locus_tag="VYS74_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV3788406.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            44603..45031
                     /locus_tag="VYS74_000320"
     CDS             44603..45031
                     /locus_tag="VYS74_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3788407.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            45169..46119
                     /locus_tag="VYS74_000321"
     CDS             45169..46119
                     /locus_tag="VYS74_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV3788408.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            46121..46864
                     /locus_tag="VYS74_000322"
     CDS             46121..46864
                     /locus_tag="VYS74_000322"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV3788409.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            46875..48671
                     /gene="pepF"
                     /locus_tag="VYS74_000323"
     CDS             46875..48671
                     /gene="pepF"
                     /locus_tag="VYS74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3788410.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            48918..49709
                     /locus_tag="VYS74_000324"
     CDS             48918..49709
                     /locus_tag="VYS74_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV3788411.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            49820..50488
                     /locus_tag="VYS74_000325"
     CDS             49820..50488
                     /locus_tag="VYS74_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV3788412.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT        15985 a         8172 c        10658 g        15784 t
ORIGIN      
        1 tatcgcttac caacatttgc ttttatattt gctaaacttt cagaaaaaaa gttggagtat
       61 atgatgctag gtgactgcgt catgttagta aacgaaatgg aaataacgga tcatagagta
      121 gataacttat ttgaaaaagg gaaaaatgag ataaaagatt caattggtac aaatagtgtt
      181 ttaaataaaa aaattatttt acaaaaaatc agaaaattat ctaatcaacc gagtgggtac
      241 tggataggtt cgttggatga gagattttta gaccatgcta ttattaatca aatagatgtt
      301 actagtgaac aaattgtttt aatgagcgat ggcttttatg aattctatca aaataatcaa
      361 aataaaactt ttgaagagtt aattaagatg agatttaatt cttctgctat tgatccaatc
      421 tatggaaaga aggatgatgc aagtattcta gtcattgatg tttgatatat tgtttaaaat
      481 attaattgtt actattgaac tttgttaaga agaggtacct aaatgctaag gttatattta
      541 gaaaatgaaa ttatagaatt atcaaataat ttagagacca tttgggtatc tgtgcttgat
      601 aaatatgaga agttatttga atatttatca aatgaagaac gaatagaatt gattaaagaa
      661 gatttaatca taaatgagtc agttctaaat attgataaaa aaggatatga gttaatttgt
      721 ttacaatatc ctgtttcata tgacttaaga agaattatta gtgtaataaa aatatcaact
      781 gacattgaac gtattgggga tagaattgtt gaaattttaa aaaatctaca gattcttcaa
      841 aataatgaaa ttttgaagaa aattatttca gaaataaaaa tacttcatga agttatcgga
      901 ctgcatatga atagggctat atcttgttat agagaggaac aatctggatg tttagatatg
      961 gttgttatac aaaaacagaa tgagatagaa gagcttagta taaatatgga aaagaaaata
     1021 atgaattaca tatttgaaga tgatgggaac gtttctgaag taattagtgc tttagatatt
     1081 attcatcacc ttgataaaat tgctcatact acgcaagcaa tttataaatg gataatgtat
     1141 agaaaatatg ggaacataaa ttagaaagaa aaatatatga aaacaattat tgataatttt
     1201 gttgatagag taattgaata cggaatgtat aatgaaattg acaaaatata tgtgaaaaat
     1261 agagtactag cgttaatagg tgaggaggga attgatcgca tttccgatga aaatgacctg
     1321 aaacaaataa aagattactt agtggaaatc gccttaaaaa acggtaaaat taaagattta
     1381 atcgaggaaa aggaatgttt aggagcagag ttaatgaatt tcatagttcc tttacctagt
     1441 cgattaaatg atattttttg gagttcatat gatatatcac ctcaagaagc agttgaagag
     1501 ttttataagt taagtaaaga tagtgattat attaaaactt ctgctattgc taagaatatt
     1561 gagtttcgag catcaactaa atacggagag ctagagatta cgataaattt gtcaaaaccc
     1621 gaaaaagatc caaaaacaat tgctgctgaa aaattagtaa aagcaactaa ttatcccaaa
     1681 tgtttactat gtatggaaaa tgaaggatat caggggcgaa taaattatcc agcacgttcg
     1741 aatcatagaa ttattcggtt gaaacttggt gacgaagtat ggggattcca gtattctccc
     1801 tactcatact ttaatgagca tgcgatattt ttgaatagtc aacatgtacc aatggctatt
     1861 acctctaaaa catttgaaca attattggag attgttgata ttttgccagg atattttgct
     1921 ggttcaaatt ctgatcttcc tatctcagga ggctccattc ttagtcataa ccactaccag
     1981 ggagggaaac atattttccc aatggaaaaa gcaaaatttg agagtgaatt ttgttttaaa
     2041 gactttgagg atgttaatgc tggtattgta aaatggccaa tgtcagtaat taggttacaa
     2101 agtgaaaata aaaatcgctt gttagattta gctactaaaa ttctaaataa gtggagagaa
     2161 tattcagatt tagaagtaga catcattgct atgactgaag atgtcccaca ccatacggtg
     2221 actcctattg ctcgtaaagt ggacgggaga tatgaattag atattgttct aagagataat
     2281 catactactg aacagtaccc agatggtgtt ttccatcctc atcaagatgt acaacatatt
     2341 aaaaaggaaa atatcgggtt aatcgaagtg atgggacttg cgatattacc acctcgtttg
     2401 aaaccagagt tagaagaagt gggaaaatat cttttaggag aggaaaacgc aattgcggat
     2461 tatcatttag aatgggccga ccaattgaaa gaaaaatatc ctcgcattaa taaagaagag
     2521 gttaataacg tagttcaaca cgaagcagga caagtgtttg cgagagttct ggaagatgca
     2581 ggagtttata agaatacacc gtctgggcat gaagcgttta tgagatttgt taagtcggtt
     2641 ggaattaatt aggaggaaac ccaaatggca atattggtaa caggcggagc tggttatatt
     2701 ggtagccata ccgtagtaga attactaaat ttaggaaagg aagtcatcat tgtcgacaac
     2761 ctttcgaact ctagcatctt ggtgctagac cgtattgaag caattacagg aatacgtccc
     2821 gtgttttacg aattagatgt ttgtgataaa caagcattga gaaaggtatt tgaacaagaa
     2881 tcgattgatg ctgcaattca ttttgcaggt tataaagctg tcggagaatc cgtgcaaaag
     2941 cctgtgatgt actacaaaaa taatattatg agtacattgg cacttgttga agtgatgtca
     3001 gaatttaatg ttaaaaagat tgtcttttct tcaagtgcga ctgtatatgg aattaacaat
     3061 cagtcacctc taattgagac gatgcaaaca agtgcgacaa atccttatgg gtatacgaaa
     3121 gtgatgcttg agcaaatttt aaaagatgtt catgtggcag attcagaatg gagtattgcg
     3181 ttgcttcgtt atttcaatcc aattggtgct catgagtctg gtttgattgg ggaagatccc
     3241 tcaggaattc ctaacaactt gatgccttat attgcacaag tagcggtagg taagctatca
     3301 gagctaagtg tgttcggaaa tgattatgat acgcttgatg gtactggtgt gcgtgattat
     3361 atccatgtag tagatttagc aataggacat ataaaagctt tagaaaaagt atctgaaaaa
     3421 acagatgttt atatctataa cctaggctcg ggagaaggca ctagtgtatt acaacttgta
     3481 aatacatttg aaagtgttaa taagatccct attccttata aaatagttcc aagacgttca
     3541 ggagacgttg caacttgtta tgcaaatgca gacaaagcat ataaggaatt aaattggagg
     3601 acaacaaaat cgattgaaga catgtgtaga gatacatgga attggcaatc aaaaaatccc
     3661 aatggctata attgatgata ctgtctatat gagtgctgta ttttgaaaaa attacttaac
     3721 cttttaatga aatgcatatt taatgaaaga taaatttaaa aaaatgtttg caagtgcttg
     3781 acaaaggaaa cggtttcatt gtataatgta aacacggtga caccgatgtc aaatataaaa
     3841 aagaaggtct ttatatgaaa aacttaacaa aaattaagtt caaagagaat ggggaattta
     3901 atcatttccc tgggaataca gttgtagcaa atctttatac taaacaagat ttgatggaag
     3961 ttgttgatat tattcaatca cgttatagag aattaccatt tatcgataag tttactttaa
     4021 ctccaaggaa ttctattcac atgactgtaa ttgaattgtt gtgccatgaa aatcgtgaaa
     4081 cggagttttg gagcagtaat cttcctctag atacaccttt acaggaaata catgattact
     4141 ttgctaaaca acttgaaatt tttccattgt tggatgaaga aattcatatg cgtataactg
     4201 aaatgggaaa acaaaacata ctagttgaac ctgcagatga agcttctgca aagagattag
     4261 aagaaattcg tacttatgtc tcagaaaaag caggtgtttg tttccctaat catgatagat
     4321 atcaattcca tatttcaatt gggtatcttc ggattcctct aaccgaagag gaggaagaag
     4381 agtttactaa agtcagagca gaattaactg aaattttatt agagaagatt ccaactatta
     4441 ctgtaaaccg tattgactat actgtatttg aagatatgag acaatttgtt ccatatcacg
     4501 aaaaatttaa ataaaataat aaaaaaggag aacttatcat gaaaaaaatg aaagtttggt
     4561 ctactgtact tgcaacggga gttgctctta ctacacttgc tgcttgctct ggaggttcaa
     4621 attctacgac tgcttcttca tctgaagaaa aagctgataa aagtcaagaa ttagttatct
     4681 attcgaactc agtctcaaat ggtcgtggtg attggttaac tgctaaagca aaagaagctg
     4741 gttttaatat aaaaatggtt gatatcgctg gcgctcaatt agcagaccgt gttattgctg
     4801 agaagaataa tgcagttgca gatatggtat ttggaattgg tgctgttgat tcaaataaaa
     4861 ttagagatca aaaattacta gtacagtaca agcctaaatg gttagataaa attgatcaat
     4921 ctttatcaga taaagataat tattataatc ctgtgattgt tcaaccatta gttttaattg
     4981 gggcgcctga tgtaaaagaa atgcctaaag attggactga attaggtagt aagtataaag
     5041 gtaaatattc aatttatggt cttcaaggtg gtacaggacg ggcaattcta gcaagtatct
     5101 tagttcgtta ccttgatgaa aaaggagatt taggcgtatc agaaaaagga tgggaagttg
     5161 caaaagaata ctttgcaaat gcctatactc ttcaaaaagg tgaaagctca gttgttaaag
     5221 cgttagataa agaaagccca attcaatacg gtatgatctg gggttctgga gcgttaattg
     5281 gacaaaaaga acaaaatgtt gaattcaaag ttatgactcc tgagattggt gtaccatttg
     5341 taactgaaca aactatggtt ttaagcacta gtaaaaaaca agcgttagct aaagaattta
     5401 ttgattggtt tggtcaatca gaaattcaag tagaatatag taaaaaattt ggatctattc
     5461 ctgccaataa agatgcccta aaagagttac ctgaagatac gaaaaaattt gttgatcaag
     5521 tgaaaccaca aaatattgac tgggaagctg ttggaaagca tttggatgaa tgggtagaaa
     5581 aagctgaatt agaatacgta aaataagaaa taagagttat tttttatgtc caagtctctc
     5641 tattatttct aaatttaagg aagaaataaa gagaggcttt tggttttagt aatagaggga
     5701 gttagtgaga tgattaaatt tgataatatt caaattaaat atggtgattt tgttgcaatt
     5761 gataatctga atttagatat acatgaaggg gaattcttta catttcttgg gccttcagga
     5821 tgtggtaaat caactacttt gagagcattg gtaggttttt tagatccatc atcaggaagt
     5881 attgaagtta atggaacaga tgtcactcat ttggaacctg aaaagcgtgg aattggtatt
     5941 gtatttcaat cttatgcgct atttccaact atgactgttt ttgataatat tgcatttggt
     6001 ttaaaagtta agaaggtagc tccagatgtt attaaagcta aagtatcagc agtggcagca
     6061 aaaattaaga tctctgatca acagttacag cgtaatgtat cagaattatc tgggggtcaa
     6121 caacaaaggg tagcattggc tcgtgctctg gttcttgaac ctaaaattct ttgtctagat
     6181 gaaccattgt caaaccttga cgcaaaatta cgtgtagatt tgagaaaaga gttgaaaaga
     6241 cttcaaaaag agttaggtat tactacttta tatgttactc atgatcaaga ggaagccttg
     6301 actttatctg atagaattgc agtctttaac aatggataca tcgaacaggt cggtacacca
     6361 gtagagattt atcataattc tcaaactgaa tttgtatgtg atttcattgg agatattaat
     6421 gttttgaccg atgaaacagt ccacgaagta ttattgaaaa atacaagcgt tttcttagag
     6481 gataaaaaag gatacattcg attagagaaa gttcgattca atcgtgaaac tgaacaagat
     6541 tttattctaa aagggacaat tattgatgtt gagttttctg gagttacaat tcactataca
     6601 ataaaagttt ctgaaagtca gattcttaat gtaacaagta ttgatagtca ggctgctatt
     6661 agatctgtcg gagaaagtgt ggaattattt atcacaccat cagacgttct gcaattttaa
     6721 ggaggtgcag tatgcgtcat aaattaaatt taaaagattg gcttattcgt ttagggttaa
     6781 tctggttctt agtaacattt attatttatc caaactttga tctagtagtg aatgtatttg
     6841 taaaaggagg agaattttcc cttgatgctg tacatcgtgt tctaaaatct cagagggcac
     6901 ttcagagtat tatgaacagt tttaagttag cattttcact cattattaca gttaatgtcg
     6961 taggtattct ttgtgttcta tttacagagt actttgatat taaaggtgct aaaattttaa
     7021 aattaggtta tatgacctct ttaatttatg gaggagtggt tttagcgact ggatataaat
     7081 ttgtctatgg tccttatgga ttgattacaa aatttttaca aaatgttatc ccttctttag
     7141 actctaactg gtttattggg tatggtgcag tcttattcat tatgacattt tcaggaactg
     7201 ctaatcatac attgttttta acaaatacaa ttcgaagcgt tgactatcac actattgagg
     7261 ctgctcgaaa tatgggagca aaaccattta ctgttttccg aaaagtagtg ttaccaacct
     7321 taattccaac tctatttgca cttactatta tggtttttct tagtggttta tctgcagtag
     7381 cagcacccat gattgttggt ggtaaagaat ttcaaactat aaatccaatg attattacat
     7441 ttgcagggat ggggaattct cgtgatttag ctgccttact tgcaattatt ttaggtattg
     7501 caactacaat tttgcttact atcatgaata agatagaaaa aggtggaaat tatatttcta
     7561 tctctaagac taaagcgcct cttaaaaaac aaaaaattgc gtctaagcct tggaatatca
     7621 ttgctcacat tgtagcatat ggattgttca cagttttcat gcttccacta atttttatag
     7681 tattatactc atttacagat ccagttgcaa ttcaaacagg taacttaaca ttatcaaact
     7741 ttactttaga aaattatcgc ttattcttta gtaatagtgc ggcattctct ccattcttgg
     7801 tcagctttat ttattctatt attgctgcga caacagcaac aattctcgca gttgtatttg
     7861 ctcgtgttgt cagaaaacat aaatctcgtt ttgatttctt atttgaatat ggtgctctac
     7921 ttccttggtt actaccaagt acacttttag cagtaagttt attatttact tttaatcagc
     7981 cacaatttct tgtcttgaat cagattttgg taggtagttt ggtaattcta cttattgcat
     8041 atatagttgt aaaaatccca ttttcttata gaatggtacg tgctatttta tttagtgttg
     8101 atgatgagat ggaagatgca gcaagaagta tgggtgcttc acctttttat actatgatga
     8161 aggttatcat tccatttatt ttaccggttg ttctctctgt tattgcttta aactttaact
     8221 ctttattaac tgacttcgac ttatctgtat tcctttacca tcccctagct caaccattag
     8281 gtattacgat tcgatctgca ggtgatgaaa cagcaacatc taatgcacaa gctctggtat
     8341 ttgtttatac aattgttctg atgattattt ctggaacggt attatacttc acacaaagac
     8401 cggggcgtaa agtaaggaaa taatcatgac agccactagt cttgggttat caaatattga
     8461 aatagttgtc aggattgttt tatcagtagt cattggtagt ataattggtt tagagagagg
     8521 gagcaaatcc cagcctgcag gcatccgaac ttatagtatt gtttgtctag ctgcatgttt
     8581 gattatgatg acgaatgaat acgtatctta taaatttggg acaggagatc ctacacgatt
     8641 aggagctcaa gttatatcag gtgtgggttt tctaggcgct ggaacgattc ttattacaga
     8701 taaaaagaaa attacaggtc tgacaactgc agcaggcatt tgggcttcgg caggaattgg
     8761 attagctatt ggagtaggtt tttatgaggg agctctttta gtagccattt ctgtttgggg
     8821 tgtgatatcc atgttccaac cactaaaaaa atatctgcaa aatcgttcta aaatgattga
     8881 attgtatata gtggttaaat ctacagaagc ctataatcgt gtattggttt attgtgcaga
     8941 aaatggtatc agaatgaccg attcaagaac agcgtttgga gatgttaatt ctgacagaat
     9001 tgaatacttt gatgttccgg acaaaaaaat agcgtcattt attactctgg aattatcagg
     9061 tagatttgaa catcttcgac tgatggaaga aattgctaat attgtaggtg ttatttatgt
     9121 cgaagaaatt agctgaaatt tagtaaacaa agggacagat gtttttgtcc ttttgctttt
     9181 tagatgtttt aatcatctaa tttttgagta gatttttgct cagaggtttt tatgaaagat
     9241 tataaaatca attttgattt aggtaaaata gaatattttg ataataattg tttgatacaa
     9301 gtatacaaat ttatttcttt ttatgatatt tgtgaaatgg tgtttgcctt tcatttacct
     9361 cctgatgagt tgataacaaa tgttatcttt aaagaaaaaa ttaactctat gttaaaatgt
     9421 tacattgaca ggctattata tgtttttata aatcctacac attttactga aaaagttaat
     9481 ctacaatttt atggcagttt tttctcatat gaatttattt gtcgtgaagt tggaaacatt
     9541 cttaaaaata aaggtgtgaa atgcaattta aacttttttg aaggggaaga atatttgtaa
     9601 aattaatttt gtgaatttgt ggatcttaaa taatttacta aattctcttg ttaaaaaaaa
     9661 ttaaatattg tatagtaata ttagaatgtt tgtaatagag gactgaaatg aaaaatataa
     9721 atgggaaaaa ggtaactatt tatgatattg cacgtttatc aggtttttct ccaaaaacag
     9781 tttcacgagt tattaatggt ggagtaaatg ttaaagaaga aacttatcaa gcgattcaaa
     9841 aagttataga ggaattgtcg tatattccaa atgcgtatgc aaaaaattta actaaaaaag
     9901 aagctataaa tatattgatt tctgtaaaaa aaatcgactc ttttccttta atttggtttc
     9961 atacgctgtt agataaagtg ttacggacgt gtaaagaatt tggtgttaat gctatagttg
    10021 agtactttgg agaggaggat acaattagta attcaattat atcaagcaca ggtagcctag
    10081 tagatggtgt tattgttttt tatgaaagtg tagatgatat cagaattcag tatttaaaga
    10141 agaaccatat gccttttctt gttttcggtg aatctcaaac gtctggagta gtctatgtat
    10201 ctaataataa ttttcaagct acttatgata tgatgaaagc tgtaactgaa gaaaaattta
    10261 aaaatatgtg gttgcttatg ggaggagaat ctcatgttaa taaggatcgt gaaagaggtg
    10321 tgcgttcttt tctgaatgat aaaaattact ttatggattt aaaagtcatt tatggcttat
    10381 ctacaattga ttcagtttat tcttatgcaa tgcagcactt gactacccaa aattatccgg
    10441 acattatttt tgtttcagga gatgaaaagg ttcagggctt gattcgtgcg tgctatgaga
    10501 aaggaattct tattccagat gatatttcta ttattggatt tgataatatt cctatttctc
    10561 agtattatac tccagctttg tctacaattg ctcccaatta tgttaaattg gctaaagaaa
    10621 tgatagaagg tgttttagca attattaaag gggaaagtgt cacatctgtt gaagtttctc
    10681 ctaaatttgt taggagacag agtttctagt attgtttaat tcttgaaaat cctagtggaa
    10741 aagatggagt gttctaaaat aagttctgtt tagcctccat tcagtttcta ttgagttgga
    10801 tccaatttct aacaagtggg acatgttcat gttcgatcta aggaagacat ggtcatattc
    10861 gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa agaaaaaaca aagtattaaa
    10921 ataagttcaa caaatcagaa aagtaactat ttttgatctt cagggagcct gtggtgattg
    10981 tgaataggtg gttggaagta gtgaaagtgg gctgattttg aaaatgaatt tgaaacaatg
    11041 aaaattcggt gcgcacacct tacagtgcag cttgttgtta aacaaggcag agatgtaaag
    11101 ggatagtccc tttataattg aggtggcacc gcgttaccaa cgccctcaca cggaagtata
    11161 ttctgtgtgt gggctttttt ctatccgtcg tttggtttat cttttattag ggccttaagt
    11221 cgctttgatg aacttgagtt ctatctacag ctccatgcct actactaaaa gcaaacaaaa
    11281 aggctggact aatacgaaaa aagctagtaa tactcaatga aaatcaaaga gcaaactagg
    11341 aaactagccg caggctgctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc
    11401 agtaaccata cctacggcaa ggcgacgttg acgcggtttg aagagatttt cgaagagtat
    11461 aaaggtgtac cgagatgaac atctcttcat caatgtctct ggtcgtggag gcaaggacct
    11521 agctgcgatt gcagattact agaagctaaa aaataataga tggaaaaatt acagttcttt
    11581 ctctcacaga gagcttgtgg ttgctgaaaa caagcagaga aagctgtaaa gttgggttca
    11641 tctgaaacga gagaagaaaa catcattctc aagggagtgc cctttatcgc acagcctgtt
    11701 acaggagata tctgagacag gaatgagaga taggagaaat cctataattg aggtggcacc
    11761 gcgaatttcg tcctcacgca agttattttg cgtggggatt tttcatacag tcaccacgga
    11821 ctagaagtag aactgaaagg gaaattacaa tggaacgaat cattcatgga gatgtcttat
    11881 caccaatctt ggcttatatg cgcttaaagg ggcaacataa ggttatttta gagagtattc
    11941 cgagagacaa ggaaacagct cgtttttcta tcctagccta taatccagtt tttgagatta
    12001 agtttgaaaa tggggttctc tatcaaaatg gtcaagtgat tgatcgggat cccttggatt
    12061 tcctttatga agtgattcat aagagtcagc accactcaga gctacctttt ggtggtggag
    12121 ccattggttt tgtgggttac gatatgattt cgctttatga agaaattggt caaatccctg
    12181 aggatacaat tgggacgcca gacatgcatt tctttgtcta tgagagctat atggtctttg
    12241 accacaagaa ggagaagata catgtcatag aggatgcact ttatagtgag cgcagtcaag
    12301 aggccttgga aaaatccttg aaccaagtgc ttgaggaatt acgcattcct gctccaaatg
    12361 aatttgaaga tttggatcta tctccgttag acttcaaacc gcatatcgct ccacataagt
    12421 ttgagggaat ggtagaaaca gctcgtgact tgattcgtaa cggagatatg ttccaatgtg
    12481 tactcagcca gcgtttttca gcagaagtta ctggaaatcc atttgacttc tacagaaatc
    12541 tccgcgtgac taatccttct aattaccttt atttctatga ttttggggat tatcaaatca
    12601 tcggagctag tccagaaagt ttagtttctg tcaaaaatgg catcgtgaca accaatccga
    12661 ttgcagggac gcgaccaaga ggggctacgg atgaagaaga caagaccttg gcgactgacc
    12721 tcctctctga tgagaaggaa acagcagaac atcgaatgtt ggtagacttg gggcgtaacg
    12781 atattggccg catctctgaa acggccagtg tccaagttac taagtatatg aaggtggagc
    12841 tcttccgcta cgtcatgcat ttgaccagcg tggtcaaggg gcgtttgctt ccagaactca
    12901 cggccatgga tgccttgaaa gctacacttc ctgctggaac cgtttctgga gcaccaaaga
    12961 ttcgggcgat gagacgcatc tatgaactgg aaacggaaaa acggggcgta tatgcaggag
    13021 caatcggcta cttgtctgcg acgggtgata tggatttggc cattgccatc cgaaccatga
    13081 ttctcaaaaa tcaaagagcc tatgtgcagg ctggggcagg gattgtctac gactctatcg
    13141 cccaaaacga ataccaagaa accattaaca aagcaaaatc tatgactaga attggagaac
    13201 taagaccatg attttattga ttgacaacta tgattctttt acctataact tggcccaata
    13261 cattgggaat tttgcagaag ttcaggttct gagaaatgat gattccaagc tgtatgaaga
    13321 agctgaaaaa gcagatggtc tggtcttttc tcctggtcct ggttggccag ttgatgctgg
    13381 aaagatggaa gacatgattc gtgattttgc aggcaagaag ccgattcttg ggatttgttt
    13441 gggccaccaa gccattgcag aagtctttgg tggtaagcta ggtttggctc caaaagtcat
    13501 gcatgggaaa cagagcaata tcaactttga agcgccatct gttttgtatc aaggtattga
    13561 ggaaggacgt gcggtcatgc gttatcacag tattttgatt gaggaaatgc cagaagactt
    13621 tgaagtgaca gctcgttcga ctgatgacca agccatcatg gggattcaac ataaaaacct
    13681 accgatttat ggcttccagt accatccaga gagcattgga acgccagacg gcttgtcttc
    13741 tattcggaat tttatcgaga aggttgtaaa gtgaggaaac taggatgaaa gagattattg
    13801 aaaaactagc aaaatttgaa aatttatcag gtgtggaaat gacggatgtc attgagcgta
    13861 tcgtaactgg gcgtgtaact gaagcgcaga ttgcttctct cctcttagct cttaagatga
    13921 agggggaaac acctgaagaa cgcacagcca ttgcccaagt catgagagga catgcccagc
    13981 atattccaac tgagattcat gatgccatgg acaactgtgg tacaggtggg gacaagtctt
    14041 tcagttttaa tatttccaca actgcagcct ttgtcttggc tggtggcggt attcacatgg
    14101 ccaagcatgg taaccgctcg atttcttcta aatctggttc cgcagatgtc ctcgaagcct
    14161 tgggaatcaa tcttgacctc aaaccagctg aactaggtaa ggtctttgat aaaactggaa
    14221 tcgtctttct cttcgctaaa aatatgcacc cagctatgaa atacatcatg ccagctcgtt
    14281 tggaattggg aattccaacg attatgaact tgactggtcc actgattcat ccaatggctt
    14341 tagaaacaca gcttcttgga attagtcgtc cagaactcct agaaagtaca gctcaggttt
    14401 tgaaaaatat gggtcgcaaa cgtgccatcg tggttgctgg accagaaggg ttggatgaag
    14461 ctggcttgaa cggaacaacc aagattgcac ttcttgaaaa tggcgaaatc agcttgtcaa
    14521 gctttactcc agaggatttg ggaatggaag gctatgctat ggaagatatt cgtggtggga
    14581 atgctcagga aaatgcagaa attttgctta gcgttctgaa aaacgaagca agtccattct
    14641 tggaaacgac agtcttgaat gctggtcttg gtttctatgc taatggtaag attgatagca
    14701 tcaaggaagg agttgccttg gctcgtcaag tgattgctag aggcaaggcc cttgaaaaac
    14761 tcagactgtt acaggagtac caaaaatgag tcaggaattt ttagcacgaa tcttagagca
    14821 gaaggcgcgt gaggtggagc agatgaagct ggagcaaatc cagcctctgc gccagaccta
    14881 tcgcttggca gaatttttga agaatcatca ggaccgcttg caggtaatcg ctgaggtcaa
    14941 gaaagctagc cctagtttgg gagatatcaa tctcgatgtg gatattgtgc aacaggccca
    15001 gacttatgaa gaaaacggag cagtgatgat ttcggtgttg acagatgagg ttttctttaa
    15061 agggcatttg gattatctac gggaaatttc cagtcaggta gagattccga cgctcaacaa
    15121 agactttatc atagatgaaa agcaaatcat ccgcgctcgc aatgcaggtg cgacagttat
    15181 cttgctcatt gtggcagcct tgtccgaaga acgcctcaag gaactgtatg actacgcgac
    15241 agagcttggt ctggaagtct tagtggagac tcacaatcta gctgaactag aggtagccca
    15301 cagacttggt gctgagatta tcggggtcaa caaccgcaac ttgactacct ttgaggtcga
    15361 cttgcagacc agtgtagatt tagcccctta ctttgaggaa ggtcgctatt acatttctga
    15421 atctgccatt ttcacagggc aggatgcgga acgactagcc ccatacttta acggaatttt
    15481 ggtagggaca gctcttatgc aggcagaaaa tgtggcccag agaatcaagg agttgcagat
    15541 tgacaaaggt taaaatttgt ggactatcga ccaaagaagc ggtggaaaca gccgtttcag
    15601 caggagccga ctatatcggt tttgtctttg cacctagtaa aagacaggtg actttagaag
    15661 aggcagctga gttggcaaag cttattcctg cagatgtgaa aaaggttgga gtatttgttt
    15721 caccaagtcg ggtagaactg ctggaagcga ttgacaaagt tggcttggac ttggttcaag
    15781 ttcacggtca ggtggcagat gatttatttg agaatttgcc ttgtgctagc attcaggctg
    15841 tgcaggtaga tggagagggt catgtgccca attctcaggc agactatcta ctctttgatg
    15901 cccctgtggc tgggagtggc cagccctttg attggggtca actggatacg actggattac
    15961 ctcagtcttt ctttatcgca ggtgggctta atgaagacaa tgtagtaaaa gcaattcaac
    16021 actttactcc ctatgcagta gatgtatcaa gcggagtgga gacagatgga caaaaagatc
    16081 atgaaaagat tagaagattt atagagaggg taaagaatgg catatcaaga accaaataaa
    16141 gatggatttt acggaaaatt cggcggacgt tttgtcccag aaacattgat gacagcagtt
    16201 ttggagttgg agaaggccta ccgtgaaagt caggcagacc caagtttcca agaggaatta
    16261 aaccaacttt tgcgccagta tgtgggacgt gaaactcccc tttactacgc aaaaaacttg
    16321 acccagcata tcggcggagc caagatttat ctcaaacggg aagaccttaa ccatacagga
    16381 gcccacaaga ttaacaatgc cttaggacaa gtttggcttg ccaaacgcat gggtaaaaag
    16441 aaaattatcg cagaaactgg tgctggtcag cacggtgtgg caactgcaac tgctgcggcc
    16501 ctctttaaca tggaatgtac catctacatg ggtgaggaag atgtcaaacg ccaagccctc
    16561 aatgtcttcc gtatggagct tttgggagct aaggtcgagg ccgtgacaga tggttcgcgc
    16621 gtgctcaagg atgcggtcaa tgcagctctt cgttcatggg tggctaatat agatgatacc
    16681 cactatatcc ttggttctgc cttggggcct catccatttc cagaaattgt tcgtgacttc
    16741 caaagtgtca ttggtcgaga ggctaaacaa cagtaccgtg acttgacagg acaaaatctg
    16801 ccagatgccc tagtagcctg tgttggtggt gggtcgaatg ccatcggtct cttccatccc
    16861 tttgtagaag atgagtcagt agccatgtat ggagctgaag cggctggact tggtgtggat
    16921 acggagcacc acgcagctac cttgaccaag ggtcgtccag gtgtccttca cggttccctc
    16981 atggatgtgc tccaagatgc ccatggtcaa attcttgaag ccttctctat ctcagcaggt
    17041 ttggactatc ctggtatcgg tccagagcat tctcactacc acgatattaa acgtgccagc
    17101 tatgtccctg tgacagacga agaagccttg gaaggattcc aactcttgtc tcgtgtggaa
    17161 gggattatcc cagccttgga atctagccat gctatcgcct ttgcggtgaa attggccaaa
    17221 gaacttggac cagaaaagtc tatgattgtc tgcctatcag gtcgtgggga caaggatgtg
    17281 gttcaagtca aggaccgctt ggaagcagat gcagcaaaga agggagaagc tcatgcctaa
    17341 gacactaaca gaaaaattaa atgctattaa agcagctgga aaaggaattt ttgttcccta
    17401 tatcatggct ggtgaccacg agaaaggttt ggacggtctt gctgaaacaa ttcacttttt
    17461 agaagatttg ggtgtctctg caattgaagt gggtattccc ttttcagacc ctgttgcaga
    17521 tggacctgtt atcgaagaag ctggcttgcg cagtttagcc cacgggacct ctacccaggc
    17581 tttagttgaa accttgaaaa ccattgaaac agagattcca ctggtcatca tgacctactt
    17641 caaccccctc tttcagtacg gtgtggagaa ctttgtcaaa gatctggctg atacagctgt
    17701 gaagggcttg attatcccag acctgcctca tgaacatgcc aactttgtag aaccattttt
    17761 ggcaaataca gatatcgcct tgattccttt agtaagcttg accacaggaa ttgagcgcca
    17821 gaaagagttg attgcagggg cggagggatt cgtctatgcc gttgccatca atggggtgac
    17881 agggaaatca ggcaattacc gtgcagattt ggacaagcac ttggcgcaac ttcatcaagt
    17941 ggccgacatc ccagtcttga caggttttgg tgtatctagt caagccgatc tggaacgctt
    18001 caatgcggtg tcagatggtg ttatcgtcgg ttcaaaaata gtaaaagctc tccaccaagg
    18061 agagccgatt caggatttta tcagacaagc agtagcttac caaaaataat caaacaagta
    18121 gcaagtaaga ggaaaggcac gaaaggaagt ctttcctttt tcttttgcag gagaaaggtc
    18181 agttttatag tgaattgaat ctggaatatt acactacggt tcctaaaata ttttcagaaa
    18241 gcgatttgaa tttcccaata aatttgttca gtttttaatt gattttacta tagaagatat
    18301 aattttctaa attactctga cacagaactt taagaaaaag ataattattg tagtaatttc
    18361 tttaagaaaa tgatataatt cattaaaaag tatttggagg aggaagaatt atgttgaatc
    18421 aggatctctt tgattcgctt gaggcacaaa aaattgtaga tactttgatg aaagggcaaa
    18481 aagattatgt agatgaacgt ctagaaaaaa gagagacaat gatagtgtcg aatggttatg
    18541 catggacgcg acctaatcat attgatactg catttgcatc agcagatttg tttgagtata
    18601 aattacaatt agcaggacag acttggggat atttagaatt tgaaacaaat acagaaaaat
    18661 atgggaaagt attgttaatt ataaagggta agaagcgact tacgaaccaa tttcctttgg
    18721 tacaaaaaaa taagagtggc tacttatttg aatacgctca gatgaataca ctttatctta
    18781 atcaacattc ttcctacaaa aatgatgaag atagtcattc ctttccaatt cagatggagt
    18841 tagtttctga tgaaatgatt caagaaattg aacaagctac taaaaattcg aatatcgaaa
    18901 aatttatgat tttaacttat gaggcggact cagaaaacaa tattatatct gtagatgttg
    18961 ttatgcctga tgcacgaact ggtcagctac acttgattca agatttgtct gagtatattc
    19021 aatcaagttc gtaccatttc gaagaagcta aatatcaaga tattcctaat ttttcagaat
    19081 tatcggaaac agaagatttt gaaattattc caagaataga aaaacaagaa ggtcaaaagt
    19141 agtaaggagt atataaatgt ttaatggtcg ggtattgaaa gaattacggc tgttaaatgg
    19201 tttaagtaga gcagaattag ctcagagaat taatttaacg gaacaagcca tttggcagtt
    19261 tgagtccaac gaaacgaaac ctaaattatc aaccaaaatg catttggcca accaatttca
    19321 tgttgattta acttattttg aacaggaaga agagagcatt cgatttgatt cttctgtaat
    19381 tgcctttaga aatgcagacc tagcaacacg gaaaacaata gatattcaaa ctatgtattt
    19441 acataaggta gatagtttga ttgattattt tgaaagtttt gtaattatac ctaatattat
    19501 aattcatgac ctaagtaatg tagtgagtga atcttatcat aagggagaat ccattgagga
    19561 attggctctt tatgccaggg aaaaattagg tatttcaaaa gataatcatg atttgcttta
    19621 taaattagaa cgttcaggta tctatatcgt ggaacgatta attaatggcc aagctgatgc
    19681 ttatagcgca tggtcaaaat tgggaagacc ttatattgtg ttgggaacga ataaatcatc
    19741 tgtacgtcga aattttgact tagctcatga gctaggacat attcttttac ataaatataa
    19801 ggatatgaat gaagatggcg atcgtttgga gcaagaagca aattattttg catcatgttt
    19861 tttattgcca aaagaagagt ttttagtcaa atttgaagag agggttggca agcgtgccag
    19921 caatcctgat agttatattt tattgaagtc ggatttgaat gtttcgatac aggctttaga
    19981 gtatcgagct tttaagttag gattattgac tccaaagcaa cattcttact tttatcgtca
    20041 aattgcgcaa aaaggttaca aaatgattga acctttggat gatcaaattt ttgttaaaaa
    20101 accaagcaaa gtaaagagta ttctggacgt cgttttgagt aatcatctag tcagtctagc
    20161 gactataatg tctaaacaaa gtatttgttt acagtttata agcgaaatat tttcagtcga
    20221 aatgaaattt tttgatcagt atcaagaaga tagaagaaca gatcgatttg ataacatcat
    20281 ccctttgtac aaaagaaata atttataatt taactgtgaa ataatttcat gaaggacata
    20341 tggcagaaat tggattttat cagacaagca gtagcttacc aaaaataatc aaacaagtag
    20401 caagtaagag gaaaggcacg aaaggaagtc tttccttttt cttttgcagg ataaaggcca
    20461 ggatacccgt cgcagaagca aactgaatca agatcagtaa ctccgttacg ctaaagacga
    20521 gagcacaaga agctaaaaag aggaaatctc ctgcgcccat gcggatatcg ataaaatgag
    20581 ccaaaattcc aagagcaagg aaggagacca tgaccagatt ccagccagag gaagctatta
    20641 ggattaggtg gaaagtcatc cagaccagta agggatattc ctgatggtga aagtcgtaga
    20701 tacccaaggt caaaccagcg gtgattagga cgacttgccc caaggaaagc aattcccaag
    20761 agtaaagcag aaagaggagt cctaagacta attcaaagag ggcataccag acaggatagc
    20821 gaactttgca gtagcgacag cgaaagcgat tgaagacctg tgagagaatc ggaatcaaat
    20881 ctaagggacg caagcgagtc tgacaggaat cgcagtgact ggctgaactg ataatggatt
    20941 gctctggaaa acggtcaatg accaaaccaa gaaaggaagc gagaatgctc ccgacgagaa
    21001 aaaaataaaa atcaatcata cttatctatt cgtaaaaaat gggagaaata gtataatgga
    21061 gaaacataga taagatggtg aggaaaagat gacaattcgt tttgaagaaa aggtgagtat
    21121 agaaaacgct caatttgtat gccagtggtc caactcccta ggcaaatcct ttcaagaaca
    21181 atggatggga ccaaggattc cttttctact gacccttcaa gccttggaag gagtcttttc
    21241 aatctttgat gaacaagagt ttgtgggact tatccagaaa atcaggctag aagacagcaa
    21301 tcttcatatc gggagatttt ttatcaaccc ccagaaacag gagcaagact taggtagcca
    21361 ggctttaagg aaatttgtta gtttggcctt tgaaaatgaa gatatagata gtatttctct
    21421 aaatgtcttc gaggcaaatc aaagagctca gaatctttac caaaaagaag gatttgaaat
    21481 cgttcaaatg gttgaagcac ctgtacgaaa atatagtaga ttgaaactag aatagtacac
    21541 ctctacttct aaaacattgt tagaaatcga tttgactgtc ccgatcgatt cgtcctgtta
    21601 ttatttcatt ttactataca ttatgaaaaa ggggagatag gagtataatt ccaaatataa
    21661 agaaagcgaa caaaacagaa ttctgatttt cagaaaacag gtttattcgc tttttatgat
    21721 atactccctc tttttagcct atttattaaa tgtaatagaa aattaagaat gtagaattgt
    21781 atgatacaaa cagagtgcaa tagctgagtg tgcggctgta aaatcattgt aaggttcaat
    21841 aatgacttca ggtaggcgat tatagagcag tttgggtttg tagttcattt cactatatag
    21901 tttttggatg atgtgttgat ttgttagtaa aggactatgt aggtagattt tttgagagtc
    21961 aatcatcata ctgatattta aaatggtttg actaagatag agtaaggctt gatggataag
    22021 tgtgattatt ccagtgtcac ctaattgata agcggttaaa ataacttgga tatcaatatc
    22081 atcggcattt ttgactaaac taggaagtag agaataggga gattgatgat atagaatttt
    22141 tgattttttg attaaccaag attctccagc aaaagtttgt aagcatccct tgcgcccgca
    22201 gctacattct tctccctcag atgaaacaac tgtatgccct atttctccaa tcattagatt
    22261 tcctttccca taaatgtttc cgtcgtagat ataagaacag tgcatacctc tagcaaaatg
    22321 aaaataagca aaattagggt cattctgttg gcgactaaat aggcgtttac ctatagccat
    22381 acagttaaca ttgttagaga aaaacaaagg cttatcaaag tgggattgaa tcatttctaa
    22441 gtcgatatgt tgccataaag gattgtttgt tgttatttta taattatcta aatagcgacc
    22501 aggtagcgcg attccaattg cttctatttc ataatcagag cagttattta agaattgctt
    22561 tagtgtctgg ttaatgagtt gatttccctt ttcttggatt aactgtttag taacaatttc
    22621 tttctcttcc tttaagatat ttcctagatt atctcctaaa gcaaaagtga aatgtttttc
    22681 agatagttca caaccaatgt agtaaaatcg tttagcttga atgtctagca ggattttttt
    22741 tctccctacg cttgtatcgt gttcgtcttc accaagttct aagagaatat tttctttaat
    22801 cagatcattt gtgatactgc tagttgttgc aggtgttatt cctgttttag tagcgatttc
    22861 aattcgtgaa atgggaccta atgtgtaaac tgtttccagt attttagctc ttagttgtaa
    22921 ttgtttctta gataaggtca tattagtttt cttcctagtt gtcagattct taaaatccta
    22981 tgattttttg ctatggataa atgtagatgt taattttttt aagcttttca ttacttactg
    23041 gtctcttttt attatatatt gataataaaa aataatcaaa tgtttacaca aatccttgac
    23101 aaggctttct tatagttgta aaatataaat aacaaaagat aatcaaataa caacttgacg
    23161 tcaaaaaata aagataattg ataacttttg ataataaaat atatttaaga ggaaaaggtt
    23221 atggaaaaat tattacaaga aaagttactt ccagtagcgg ctagattggg taataataaa
    23281 gcgttggttt ctattcgaga cggtattact ctaactatac ctttattgtt gataggttca
    23341 ttactaatgg ttattgcaag ttttcctatt ccaggatggg aaaaatatct gggtgatatt
    23401 ggtgttgctg actatttatg gaagggtgtt gatagtagtt ttggattact tggtcttgta
    23461 gctagttttg gtatcgctta ctttatggct agacaataca aagttgatgg tattcctgct
    23521 ggtattgtgt cattatcatc ttttattaca gttacgcctt tcattagagg agaggctgga
    23581 gcaggaatgc ctaccgcttt catggcatca aaaggtttat ttgttgctat gattttagga
    23641 ttgattaatg gctatatcta ccaatggttt ataaatcata atatacagat aaaaatgccg
    23701 gatggtgttc caccagcagt atctaaaagc tttagtgcca ttattcctgg tgcagtgact
    23761 attgttggtt ggttgattgt ttatgcaact ttagataaat taagtttacc taatcttcat
    23821 gaaattgctc aagtagcttt gggaggtcca cttggacttt taggaaataa tgttattggt
    23881 cttcttatct taattttcct taatagtagt ttttggtttg taggattaca cggaggaaat
    23941 gttgttaatg cagttatgaa accgttatgg ttagctaatc tagatgcaaa taaagtagct
    24001 tatcaaactg gagaaactct tccaaatatt tttactagcg tatttatgga taattttgta
    24061 ttcataggtg gcggtggtgc aaccataggt ttagttcttg cgttaggata tttagcacat
    24121 aagaagaaag ctagtaaaca actaaaaact ttggcaccta taactgttat acctggatta
    24181 tttaatataa atgaaccagc aatgtttggc gttcctattg ttttgaacat tttgttacta
    24241 gttccattta tcttagctcc aatgtttaac ttactagtag cgtggggagc aatggcatca
    24301 ggtttggttc cgctaactta tacagatcca ggttggacta tgccacctgt tataagtggt
    24361 ttacttgcta caggaagtat ttctggttca ttattacaaa ttgtattgat tgttttggat
    24421 gtcttacttt atttaccatt tgtaatagct attgaaaaac ggtttaagtt attggaggat
    24481 tgagaatgaa acgtttaatt agtgcaaatc catctgagat attacagatg aatgctgaag
    24541 aattaaaaca aagtatttta gcaagtgaag gtagagttgt tctatctgaa aatgtagtta
    24601 ctcgtgagac atttgttggg gatataacta attctgaaat tgctagagct tttggagctg
    24661 atatgatttt attgaattgt gttgatgttt ttgagcctaa aatttatgct ttggatagtt
    24721 caggtgatga tgttattcat cgcttacacc agcttgttgc ttgtccaatt ggtgtaaatt
    24781 tggaaccgat tgacccatct gcaaagatgc tagaggaaac acaggaaatt gttgcaggtc
    24841 gtgttgctag tgttgaaaca ttgaatcgaa tagaggagtt aggttttgac tttgtctgtt
    24901 tgactggaaa tcctggaaca ggagttagca atcaagagat tatcaaagca gttcaatctg
    24961 ctaaagagaa tttctcaggt ttgattatag ctggtaaaat gcacggggca ggagtgaatg
    25021 agcctgtggc agagctttct gtcgcagagc aattgttgga agcaggtgcg gatgtgatac
    25081 ttgttccagc agttggaacc gttccagcct ttcatgacca agagttgcgt gaagtcgttg
    25141 atctcgttca tagtaagggt ggtcttgtat tgagtgctat tggtactagc caagaaacat
    25201 ctgatacaga tactatcaag gaaattgcac ttagaaataa aatttgtgga gttgatattc
    25261 aacatatagg tgacgcagga tatgggggac tggcaacagt cgataatatt tatgcattga
    25321 gcaaggcaat tagaggagtg agacatacag tatctcgctt ggctaggtca gtaaataggt
    25381 gataaaggag taagctatgg ctaaagtaac aattatgtta gcatgtgcag caggtatgag
    25441 tacaagtctg ctagtgacaa agatgcaaaa ggcagcagaa gataaggggt tggatgcaga
    25501 aatttttgca gttccagctc ctgaagcaga agaaattgta gcaacaaaag aagtaaatgt
    25561 gttgctttta ggtcctcaag ttcgctattt actaggggac tttcaagaaa aactaaaaga
    25621 tagacagatt cctgtggcgg ttattccgat gacagattac ggaatgatga atggttctaa
    25681 agttttagat ttagctgaaa gtttattaga ctaagattga ggagaatgtt atggatgaaa
    25741 gtaatttaga atctgtaatg gggctaatta tgtatggtgg ggaagccaaa agtaatgcta
    25801 tggaggctat tcaggcagca aaaaaagggg atttctctaa agccaatcga agattagctg
    25861 atgcgaatgc tgccttatta caggcgcata aggctcaaac agaaatgttg acaagagagg
    25921 cacaggggga aaaaacatca attagcctct tgatggtaca cgcgcaagat catttaatga
    25981 cgagtctgac ttttgttgac ttggcgaaag aagtggtaga agtatacgaa cgatttgaaa
    26041 aaaattagga gtttggtatg aatacaatgt tggataaaat gcaagaaaaa ctttctccaa
    26101 ttgcaatgaa agttgggaat cagaagtttt tagttgcttt acgtgattca tttgtgggga
    26161 ctatgcctgt tattatgaca ggttccattg ctttgttatt aaatgctttt ctagtggatt
    26221 taccacaaca atttcaccta gaaagtatta cgaaaacctt tcagtggctt gttgacatca
    26281 ataatttggt attcaaggga agtataccaa ttgtttcctt gttgttcatc tattgcttgg
    26341 gagtgaatat cgctaagatt tacaaggttg atacagtttc tactggtttg gtatcacttg
    26401 cttcgtttgt catttcgatt ggaagtacag ttacaaagag tttccctttg gcaaatgtag
    26461 gagatgtgaa attagatcag atcttacaag gaattgataa tctagcattt gatggtaaaa
    26521 atctgatggt aactattggg aatgtgattc caggaaacca tattaatgcc agaggcagaa
    26581 agggagggat atcctcatcc tgctggggct ccgtggtggt tacaagttcc gaacgcttgc
    26641 tggaaacatc cctacggtga gaacagtaat ctagtgggtt tagaagacca tccagtagtt
    26701 catgttgctt tagaagatgc attggctttc tgtaattgga gcggaatgtc tttaccgact
    26761 gaagcacagt gggaatatgc tgctagagga ggaagacaat ctgaatatct ttggggagat
    26821 actcttttag aaggtggata ttatcatgcc aatacttggc aaggaagatt tccttatgaa
    26881 aatactgcct tagatggatt tataggaacg gctcctgtct atgaattttt acctaatgat
    26941 tttggtctat atcaaatgat tgggaatgtt tgggaatggt gtcgcaatcc gagatatacc
    27001 ttattagcta gttttaatga agatgactat gagttaccaa aatatgggat acaagatgag
    27061 gagtatgcta tcagaggagg ttccttttta tgtcattgtt cttattgtaa tcgataccga
    27121 gtagctgcta gaaatggatg tatttctact tccactagta gccatctagg ttttagatgt
    27181 ttgaaagaat aggatggact taaaaagtgg ttgagattat ttattttcta atcattataa
    27241 tagctagtgg attgggttct atatcaggca tgggtggtgg aattataatt aagccactta
    27301 tggatagttt tggataccat tcggtcagtg atattgcttt ttattctagt ttctctgttt
    27361 ttataatggc gattatttca acaatcaaaa gattttcaca aagtaaagag ataaaatgga
    27421 gattgatttt tacggtatcc ttttcttctg ttttaggagg atttctaggt tatcttattt
    27481 ttcaagtttt gttatctcaa ttatcagtac ggctagtatc tattgttcaa atgattttac
    27541 tatttgtgat gttacttgta tcatttgttt taacagattt taaaaaaact tatcaatttg
    27601 ataagatagg attctatatg atttgtggtc ttctattggg cttgatttct agttttttag
    27661 ggattggtgg tggtccgctc aatgtatctt tattgatggt gtttttttct atttcgataa
    27721 aagaagcaac gatgtattct cttgcaatta ttttcttttc ccagctgtct catttggcta
    27781 caattgttgt ggttacaggt ttaaatcagt atcatttagc acctgttcca gttatttttc
    27841 ttgcttctat atgcggaggg gtattgggga cggttgtatc aaaagtctta cctgaaaatt
    27901 gggtaagata ctgttttaaa gggatgttat tctttgtcat gggaatgact ttatataatt
    27961 tatttcatat tttgtaaggt cagtgtgaaa atatttaaat agacttcctg taaaaactgg
    28021 atttaacgcc ctccatttgt caaattaagc tagacagtct taccaactca gaaaatacct
    28081 gatgaggtat ttggtatcca aaggatttag gtgggatgtg atttcttgag gctgggaaaa
    28141 agtctttaaa attaaaaaat gtataatatc aggtgttgga aaaactttga tactatgcgt
    28201 tttattgtgg gaatatttat ttcattttct cctgaaattg agtttttacc cagcctaaaa
    28261 ttcttgactg acttcgttga aatctgtctg ctttggcaag tcatgacacc ttaaaagtct
    28321 atttgaatgt tcgttgagac accgctgtct tggacatcct agattcgcaa aaaagctctc
    28381 actatcgtga tgattggaaa tatctttcca ttttgaaaac tcttttccac agtcaaacgt
    28441 aatagattta aagaggttca ctagaaattg tgccagccac ccttggatgg attctgccat
    28501 atcctttgcc ttacgactct tggtcttcaa actgacaatc aattgactat acctgttcgc
    28561 tcacaaatag gagagaactt tttacgaaaa tctaatgaat atgccacaag aacattatac
    28621 cgcaacgtgt actatttttg gttcatttta ctatagaaac tattgaagca cctgtacgaa
    28681 aatatagtag attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg
    28741 atttgactgt cccgatcgat tcgtcctgtt attatttcat tttactatac attatgaaaa
    28801 aggggagata gaaagcaaac agccttgaaa tcaatgttga ctgcgattat gaacctgaat
    28861 cagcccctca aaagggactg tatatgaatg aacgttacca gtgtttaaaa actaaagaat
    28921 atcaggcact tttatcttcc aagggtagac aaattttcgc taaacgtaag attgatatga
    28981 aatctgtctt tgggcagata aaggtttgtt tgggttataa gagatgtcat ctgagaggta
    29041 agcgtcaagt gagaattgac atgggattca tactcatggc caacaacctg ctgaaatata
    29101 ataagagaaa gaggcaaaat taaaaagcta gagttccgca attgggaatc tctagctttt
    29161 tgttttctga gaatcatctt gactcagact cttttgacta atgttaccaa aaaagataaa
    29221 gtaactttta accttattta tcgtcgccac tcaacatgtt tttaacacct tctacagcac
    29281 cttctacagc gtctttagcg tcttcggcaa cttcttttac tttagaaaca actttttcag
    29341 ctgctccttc tttttccatt ttttcatcac cgatggcttt cccaacacct tctttaatag
    29401 aacctttagc ttgatttaat ttttcttcta ctgacatgat aatttctcct gttatattta
    29461 ttttatctta gtttacacga gtgttttcgt gagatactac accatttgct gcacctttaa
    29521 cagcttctac accttcgcta actttttctt gaacagttga gaaaccagat ccaagaccag
    29581 atttagcttt ttcgaattgt tctgaagtga attctcctgt tgattcagca acgtcagata
    29641 cgcgatcttg aaggcttact gagtctgctt catgctgttc tttagttttg atgtcgacaa
    29701 cgtttacgtt gatttcaaca atttccaagt cagtcatttt agcaacttct gaagatacga
    29761 tttctctgat ttctgaatat aaagctggaa catttttttg gtactcaaca ataacgttta
    29821 agtcaactgc aacttgtgtt ttaccaactt ctacgttaac accacttgtt acgtcatcgc
    29881 tgttaacgat tttttcttta agatttgaga agaaaccacc atcgattccc aaaagacctg
    29941 aaacgttttc tagtgaaaga ccaatgattt tttggataac tttatcttcg taagtaagtt
    30001 cccctttgat ttcgtgagct acaacagtag catctttctt ttctacgtta gtgtttgtgt
    30061 ttttttcgtt tgacatatga aactccttta attaaattag taatttttat ttttttattt
    30121 atctatatag tttttttcga tatataatcc agctgcaact cccagtgctc ctaaaatcaa
    30181 tacaaatatt gttttgaaga agccaaagga gacaatcaaa caagccagaa atacgcctat
    30241 gagaccagcg ataattggat atcgatattg tttaagccat tccatttttt acttccttac
    30301 ttcacacgac taacagtctt tttgttttga ggttttggtt ggtaattttt tactgcaact
    30361 tcaagtttta cttgacgctc aataccaaaa aactgcttca atccattagt tatttcattt
    30421 tgaattattt ggcatctgtc agcgatgttg tctgaaggaa gaattttacc ttctacatga
    30481 acgaaacatt tattttttcg taaatttaca tgaacagttg ggttcttgat caatctatga
    30541 tcactcacca aacttctaac aaaaccttcg attgccgaat tctttaattt taatgtatcg
    30601 tttttagttt caagttggat ttccaagtaa cgtttaggat aaaacatcac aactaaaatg
    30661 gataataaaa ctaaagttga tagaaccact gtcccccaaa agacatatct agcaagatag
    30721 aattcaacta cggagttctg tctccagcta agtagatgaa tacctagatc actaacttga
    30781 tgataatcta tcaaaacagg aaggaaaatt gtcaagatta aaatacagaa aataatgaaa
    30841 catattttct ttgcttttga catatacaat cctttcgatt taacatttaa actatttttc
    30901 ccgtcattga tttaaaaata gtttgctaac tttactggcg aaaagtttac tttctacctg
    30961 taaagaatga cactacagta atcacaatcg ctgcacctac aatagatggg agcaaagcca
    31021 tttcagcgat tgatggaccc caactaccta aaagagattg tcctgcatat gccccgacta
    31081 aaccagcgaa tacatttgcg atgattccca tagaactact tttttagtga ttttacctgc
    31141 caagagacca ataagacctc cagcaataat tgatcacaac actgcctacc ctcctttttt
    31201 attttaatat caaaaaatca atttctttga ttaatactat tatataactt taatatagaa
    31261 aagtcaatta tttcgattgt tattattata taattttaaa aaatcaaatt ctttaattgt
    31321 tgctattata taatttttaa aattaaaaat ataatcttta gacttgaatt tgattctgta
    31381 ttcacgccct caatagacgg taaaataaga aaattgttta tatattgtct ccgtagtgtt
    31441 attatacgaa ataaaagatt ttaggaaaag tcgttatatc atgctatacc tattctttgt
    31501 ggtataattg caagaggttt aatccgataa tttataaaga gagaaagata ttcatgagag
    31561 atttattatc taaaaaaagt cataggcaat tagaattatt agaattatta tttgaacata
    31621 aacgttggtt tcatcgttct gaactagcag agttattaaa ttgtacagaa cgtgcagtca
    31681 aagatgatct atcccatgtt aaatctgctt ttcctgactt gatttttcat tcttctacta
    31741 atggtatacg cattattaat accgatgata gtgatattga aatggtttac catcatttct
    31801 ttaaacattc aactcatttt tcgattttag aattcatctt ctttaatgaa ggttgtcaag
    31861 ctgagagtat ttgtaaagaa ttttatatca gttcatcttc gctctatcgt attattagcc
    31921 aaatcaataa agtgattaaa aggcaatttc aatttgaagt cagtctgacc cctgttcaaa
    31981 tcattggaaa tgagagagac attcgttact tttttgcaca atatttttca gaaaaatatt
    32041 atttcctaga atggccattt gaaaattttt catcagagcc actatctcaa ttgttagaat
    32101 tggtttataa ggaaacaagc tttccaatga atttgtcaac gcatagaatg ctaaagttgc
    32161 tcctagttac gaacctatat agaataaagt ttggtcattt tatggaagta gataaagatt
    32221 cttttaacga ccaaagtttg gattttttaa tgcaggcaga aggaatagaa ggtgttgctc
    32281 agagttttga atcagaatac aatatctctt tagatgaaga agttgtttgc caattatttg
    32341 tgtcttactt tcaaaaaatg tttttcatag atgaaagtct ctttatgaaa tgcgtaaaaa
    32401 aggatagtta tgttgaaaaa tcttaccatc tattgagtga ttttattgat cagatttcag
    32461 tcaagtatca gattgaaatg gagaataagg ataatctgat ttggcatctg cataataccg
    32521 cacatctgta tcgtcaagag ttgtttactg agtttatttt atttgatcaa aaagggaata
    32581 caatcaggaa ctttcaaaat atttttccaa aatttgtttc agatataaaa aaagagcttt
    32641 ctcactattt agagactctt gaggtttgtt ctagttcaat gatggtaaat catctatctt
    32701 ataccttcat cactcatacc aaacatttgg tgattaattt gctacaaaat cagcctaaac
    32761 taaaggtttt agttatgagt aattttgatc agtatcatgc aaaattcgtt gcagagacac
    32821 tttcttatta ctgtagcaat aattttgaac tggaagtttg gactgaatta gaattatcaa
    32881 aggaatcttt agaagattca ccttatgata tcattatttc taattttatt attcctccta
    32941 ttgaaaataa gagactcatc tattcaaata atataaacac ggtctcactc atatacttgt
    33001 taaatgccat gatgtttatt cgattagatg agtaaccaaa aataacaaaa aaacgctatt
    33061 tcgcgtttga actgtacctc aaaagttaga cagaaaaaat ctaatttttg gggtgttttt
    33121 gttaagaaaa gaccttcaat catttaagca aaatgtacat ctagatttat agccctctaa
    33181 aactcttgct ttttttgtag aagatacgaa aaaagccata aaaccaaggc ttttcctgtt
    33241 ttatgtagat gtttacacat gaaaattgaa gtattttcaa aagctaattg gagtgatttt
    33301 attcatgatg ggataaatga tctggccaaa aatgattgag aaatagttgc ttaaaaagca
    33361 tttgcatgat actatatatt gaaaggaggt gataatatgg tagaacaaag aaaatcaatt
    33421 accatgaaag atgttgcttt agaagcagga gttagtgttg gaactgtttc acgtgtaatt
    33481 aataaagaaa aaggcattaa agaagtaact ttgaaaaaag tggaacaagc gattaaaact
    33541 ttgaattaca ttccagatta ctatgctaga ggaatgaaaa aaaatcgaac agaaacgatt
    33601 gcaatcattg taccaagtat ctggcatccc ttcttttcag aatttgctat gcatgtggaa
    33661 aatgaagtct ataagagaaa taacaaatta ctcttatgtt ctataaatgg tacaaataga
    33721 gagcaagact atctggagat gttgcgtcat aataaagttg atggagtggt tgccattacc
    33781 tatagtccaa ttgaacatta cttgacgtca ggaattccct ttgttagtat tgaccgcaca
    33841 tactcagata ttgccattcc ttgtgtttca tcagataatg atgcaggtgg aagagaagca
    33901 gctaaacaat taattagtaa aggatgtcag catttagcct ttgtgggcgg acataataca
    33961 accattaatg aaaccaaacg tcgtagaatt tcatttgaaa agtatgtcca agagcataaa
    34021 atacctctta gtatttttga tttggatgag acagttactg actattatgg aaagctagag
    34081 agtttcttgg aagaaaattc ttctatagat ggaattttta caatcaacga ttttacagct
    34141 ttggatgtga ttgaaatact agaaaaaaat ggcaaacgta ttcctcatga tgtccaaatt
    34201 attggatacg atggaattaa aatggctgga gatagagatt atctactttc aactattgaa
    34261 caaccattgg aagaaatggc aaaagaagcg gttcgtattt tgtttgatat aattgatgga
    34321 aagactgtta atttgcagac aatcctacca gtaaaatttg ttgaaggaaa aacaacaaaa
    34381 aatgaaaata agtcttgaca gaaagcgcta tcaatgatag aatgaattca gataaaaaga
    34441 tttattttta aaacaaaaat gaaacgtttc aaaaaaagga ggttgctatg aatagcaaag
    34501 cgaagcaagt ttctctttgg gaaagaatca agaaacaaaa actcttgtta ttgatgactg
    34561 tccccggttt agttttaacc tttatcttta aatacatccc tatgtatggg gttttaatcg
    34621 catttaaaga ttacaatcct ttaaaaggaa ttttagggag tgattggatt ggtttttctg
    34681 agtttacaaa attcatatcc tctcccaact ttggtatctt gttagccaac acattaaaat
    34741 taagtatcta tggtttattg cttggctttt taccaccaat cattcttgcg attatgctca
    34801 atcaactctt gagtgaaaaa gtcaaaaaac gaattcagct cattttatac gcaccaaact
    34861 ttatctcagt cgttgttatt gtcggtatga ttttcctctt cttttcagtg ggaggaccaa
    34921 tcaacaattt tctttctatg tttggaatga aggctgactt cttgacaaat ccagacttct
    34981 ttagaccctt atacatcttt agtggtatct ggcaaggaat gggctgggct tcaacgctct
    35041 acacggcaac attggtaaat gtagatccag ccttagtaga agcagctcga ctggatggag
    35101 ccaatatctt ccaacgaatc tggcacattg atattccagc tcttaagcct attatggtta
    35161 tccaatttgt tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga
    35221 tgcagacatc gttaaatttg ccaacttctg aaattatctc gacatatgtc tataaagttg
    35281 gtcttgtatc aggagactat tcttactcaa cagcggttgg tttgtttaat gcagtgatta
    35341 acgtagtatt gcttgttgca gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa
    35401 tttaaggagg aaagtatgaa aaattcgatt atggatacaa aatttgatag acgtatctta
    35461 ctcttaaata aaatcattat tgtctttatc gttttgatga ctttgcttcc tttactttat
    35521 atcgtcgtag catcctttat ggatcctaag gttctggtta gtagagggat tagctttaat
    35581 ccagccgatt ggactgtaga aggttaccag cgtgtattca gtgaccaatc tattctaaga
    35641 ggttttatca attctctact atactctttt ggatttgcag ctttaacagt cttgctatct
    35701 gtgtttacag cttatcctct ttctaagaaa gacttggttg gacgtcgttg gattaactac
    35761 ttcttgattg taactatgtt ctttggtggt ggtttagtcc caacttactt gctcgtaaaa
    35821 gaattgggaa tgctcaatac tccatgggct atcattgttc caggtgctgt caatgtttgg
    35881 aatattattc ttgctagggc ctatttccaa ggattgcctg aagaattagt tgaagctgct
    35941 gtcattgatg gtgcaaatga tttacagatt ttcttcaaaa tcatgcttcc tcttgcaaaa
    36001 ccaattatgt ttgttctctt cctttatgct tttgtaggac agtggaactc atactttgat
    36061 gcaatgattt atatcaagga tccaaacttg gaaccattgc aacttgtact tcgtaaaatt
    36121 ctcattcaga gccaaccagg tcaagacatg attggagcac aagcggctat gaatgaaatg
    36181 aaacgtttag ctgaattgat taaatacgca actattgtca tttccagctt gccattgatt
    36241 gttatgtatc cattcttcca aaaatacttt gataaaggaa ttatggctgg ttcacttaaa
    36301 ggataaaaaa agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc
    36361 agcagttttg ttgacagcta gtttagcagt acttgcagcc tgtggctcaa aaaatacagc
    36421 ttcaagtcca gattataagt tggaaggtgt aacattcccg cttcaagaaa agaaaacatt
    36481 gaagtttatg acagccagtt caccgttatc tcctaaagac ccaaatgaaa agttaatttt
    36541 gcaacgtttg gagaaggaaa ctggcgttca tattgactgg accaactacc aatccgactt
    36601 tgcagaaaaa cgtaacttgg atatttctag tggtgattta ccagatgcta tccacaacga
    36661 cggagcttca gatgtggact tgatgaactg ggctaaaaaa ggtgttatta ttccagttga
    36721 agatttgatt gataaataca tgccaaatct taagaaaatt ttggatgaga aaccagagta
    36781 caaggccttg atgacagcac ctgatgggca catttactca ttcccatgga ttgaagagct
    36841 tggagatggt aaagagtcta ttcacagcgt caacgatatg gcttggatta acaaagattg
    36901 gcttaagaaa cttggtcttg aaatgccaaa aactactgat gatttgatta aagtcctaga
    36961 agctttcaaa aacggggatc caaatggaaa tggagaggct gatgaaattc cattttcatt
    37021 tattagtggt aacggaaacg aagattttaa attcctattt gctgcatttg gtatagggga
    37081 taacgatgat catttagtag taggaaatga tggcaaagtt gatttcacag cagataacga
    37141 taactataaa gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct tgattgataa
    37201 agaagctttc gaacatgatt ggaatagtta cattgctaaa ggtcatgatc agaaatttgg
    37261 tgtttacttt acatgggata agaataatgt tactggaagt aacgaaagtt atgatgtttt
    37321 accagtactt gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt
    37381 tgcacgtgac aagatggtta ttaccagtgt aaacaaaaac ctagaattga cagctaaatg
    37441 gattgatgca caatacgctc cactccaatc tgtgcaaaat aactggggaa cttacggaga
    37501 tgacaaacaa caaaacatct ttgaattgga tcaagcgtca aatagtctaa aacacttacc
    37561 actaaacgga actgcaccag cagaacttcg tcaaaagact gaagtaggag gaccactagc
    37621 tatcctagat tcatactatg gtaaagtaac aaccatgcct gatgatgcca aatggcgttt
    37681 ggatcttatc aaagaatatt atgttcctta catgagcaat gacaataact atccaagagt
    37741 ctttatgaca caggaagatt tggacaagat tgcccatatc gaagcagata tgaatgacta
    37801 tatctaccgt aaacgtgctg aatggattgt aaatggcaat attgatactg agtgggatga
    37861 ttacaagaaa gaacttgaaa aatacggact ttctgattac ctcgctatta aacaaaaata
    37921 ctacgaccaa taccaagcaa acaaaaacta gaggttgatt atgggagata agaaatacac
    37981 agtagaaaaa gccaatcgtt ttatagcaga aaataaacat ctcgttaata ctcaatataa
    38041 gcctgaagaa catttttcag ctgagattgg ttggatcaat gatccaaatg gatttgttta
    38101 ttttcgtgga gaataccatc tcttttatca attctatcca tatgatagtg tttggggacc
    38161 tatgcactgg ggacatgcta aaagtaagga cttggtgact tgggagcact tgccagtggc
    38221 acttgctcct gatcaagatt atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa
    38281 ggatgatcgc ctctggctca tgtacactgg gcatatcgaa gaaaaaaacg gtgtccgcca
    38341 agtgcaaaat atggcatttt cagacgacgg gattcacttt gaaaagattg aacaaaatcc
    38401 agttgcaaca ggagcagact tgccagatga gttgattgct gctgatttcc gtgatccaaa
    38461 actctttgaa aaagatggac gctattactc cgtagtagct gccaaacaca aggataatgt
    38521 gggctgtatc gttctactag ggtccgataa cctagtagaa tggcagttcg aatccatctt
    38581 tttaaaaggg gtagaacacc aagggtttat gtgggaatgc ccagattact tcgagttaga
    38641 tgggaaagat tgccttatta tgtcacccat gcgttatcag cgtgagggag actcatatca
    38701 taacatcaac tcatcgcttt tgttcacggg taaggtagag tggggagaaa aatgttttat
    38761 cccagaatca gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt
    38821 ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag acatgggggc gtacccttcc
    38881 aacccatgac caagaacaca agtgggcatg tgccatgact ttacctagaa ttctaagatt
    38941 ggaagatggc aaactaagac aattccctgt taaaaaaggc caatatcaaa tccaaataga
    39001 taaagattgt cattaccact taggaaatga tacagattat cttgaatttg gttatgacag
    39061 taatgcgcag caagtttaca ttgatcgtag ccatcttatt caaaaaattc taggtgaaga
    39121 agaacaggac actagtcgac ggtatgtaga tattgaagct aaagaattgg aagttgttct
    39181 agataaaaat tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta
    39241 ttacttaacg gtgccagctg agctatcacg aattgattaa aaattaagtt atttctccta
    39301 aagaaaaagt tctctttcta aaatagtgga aagaggactt tttgtgtttt gggtatataa
    39361 gcttagttta tggtatttgt aaaattggtg ttggattatg atttaaacta gttttctaaa
    39421 gaatttgaaa aaaattttat ttaagcaaaa aaagccctat attataaggc tttttcctgt
    39481 tgatttagat gccccctgca gggctcgaac ctgcgaccca tagattaaga gtctactgct
    39541 ctaccaactg agctaagaag gcaaataaaa agctgtattg gtgccgaaac ttcacggttt
    39601 gtattgaacc cgcgcaatta agcaggtggg caactcgctc taactgaagc tgtttccgtg
    39661 tgagacggcc tacatgctgt tagaagactt ttgtttccct aataatacaa aaaatagtcg
    39721 gtcaacactt aagtgtgaag tcgtacacca cagcgtttct atatttatat gataccactt
    39781 tttcaaaaaa aatcaagagg aaagtgcagt tttttgaaaa ggatttcaga tttttcgcaa
    39841 atggtcgata gcttcctttc tttgagccaa agcccgttca tatttaccag tatcttctgc
    39901 ttggaaatag tgatgattac gaattttttc tggcagatag tcttgcttga cccaatttcc
    39961 aggatagttg tgtggataga gatagtcttg ggcattcccc agttccttgc ttccactgta
    40021 gtgcccatca cgcaggtgtc gcggaatagg caagtgccct gatgctttga ggtcagcaag
    40081 tgccttatcc atagctacat aggctgagtt ggattttgga gaaagggcca aatcaatcac
    40141 gacattggcg atgagaatgc gggcttctgg gaaaccaatc ctttgtgcag catccagagc
    40201 agtcacggta tgaatctggg cctcaggatt ggctaaaccg atatcttcat aggcgataac
    40261 agtcaagcga cgagcgagac taggcagatc cccagcctca atcaagcggg cagcatagtg
    40321 gagactggca tccacatctg agccacggat agacttttgc agggcagata gaacatcata
    40381 gtgaccgtct ccatccttgt ccatagtgat gtagcttctc tgaagactat tttccatgat
    40441 gtctaaggtg atgtggcgaa tgccctcgtc attctcaggg gtagagagaa cagccaagtc
    40501 cagtgagtta aaggcagagc gaaggtctcc gtttgtagag gttgcgatga aatccagcgc
    40561 atcctcatct agttctattg gaaaatcaaa accacgttca gggttactta gagctatctg
    40621 cagggcctct ttgacgtctt ggttagacag aggttccaac tcaaaaattt gaacacggct
    40681 acgaatggca ggagtgacgg aaaagaaagg attttcagtc gttgctccaa tcatgatgac
    40741 cagtccactt tccaagagag gcaagaggaa gtcttgcttg gtcttatcta gtcgatgaat
    40801 ttcgtctagc aataggacga gaccaccaga aaatttagct tcttccgaga tttcttgcag
    40861 tctcttttta ctatcaactg tcgcattgaa agttcgaaag gcatacttgg tcgttccagc
    40921 gatggcagag gcaatactgg ttttgccgat tcccggaggg ccatatagaa tcatggagga
    40981 caggcggttg gcttccacca tgcggcggat gatttttcca ggtccgacca gatgctcctg
    41041 accgatgacc tggtcgatgg ttttagggcg catgcgaagc gcgagattgt ctggcatagc
    41101 agtcctttct agcgtggatt tgagtagaat gactcatcta ctatatctta ctatgtccat
    41161 tctagcctat tagttgtgat atttcaatgg ttttgtaata atcagggagt gagatagaat
    41221 cttttttact atttaattta atgaggagag gtatcagcat gagagatttg attttcctta
    41281 aagctcaaaa taggatagtt gagcctaaag aagtggaagt gctgacttga gaaataatat
    41341 tgctgaatgt cattgtatga actataatga aaagagaggt gataagcaat ggaaatgatg
    41401 gaccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt cttagtgatc
    41461 aagttatttc agggaaaaga aaacttttct tccggaagat actccaaaag agattttaga
    41521 tttgtacgaa aacattaaat ctaaacttga ttttgcttac caagaagttc attctaataa
    41581 tgggttgaaa aaatatgaaa aatagaataa ttgatgtttt tgaagtagta aatcgcctcc
    41641 ttgtaataac tgttgaaaat cctgatttcg aagacttgag agttaatcat actcaatgaa
    41701 aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggt aaggcgacgt
    41761 tgacgtggtt tgaatttgat tttcgaagag tatcagtttg taaaaattgg agataaaaaa
    41821 tatagagtac gcagtggtcc tatgattcat tcaactccgc ctcaatctgt cttagatcga
    41881 gatactttta caattgacta tacagatgat gaattgctcg ataaagaagc agtttttact
    41941 actcattaga aaaataggta tgaataaatt ggtttccctt agaaccacat ctaggggctt
    42001 ttatgtataa tagaataata agtatcactc ctttacaata cgtgtaacac gtgttataat
    42061 ggtgttgtca ggaggtaaaa gcgctatacc tatgacacaa aaagagatgg ttaaactcct
    42121 tacggcccat ggttggataa agactagagg cggtaaaggc tcccatatta aaatcgagaa
    42181 gcaaggggaa agacctatca caatccttca tggtgagctg aataagtaca ctgaaagagg
    42241 gataggaaag caagctgggt tgtaaaattc agtccctgct ttttgatatg gaggtagtga
    42301 aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa cagaagcgac
    42361 ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg ggatttctga
    42421 ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga tagagagtga
    42481 tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata atgatccttt
    42541 taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta tttctatggt
    42601 atctgttgat gtatcagaat acctaggaag tcaggaaccc attaaaaaga ctttgaccat
    42661 accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc agactttgac
    42721 agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc gtggtaatag
    42781 tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta tagatttatt
    42841 agccctttcg agggcttttt atattgattt ttatgagaaa tgaagaaatc agacttttaa
    42901 gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg acataaaata
    42961 attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg gggcgattaa
    43021 agagtcgcat agaaagagga caaaatggca acatacggat ttttagatat tttagaggaa
    43081 gagttggaca agaactttcc ctttgacttt gagattagtt gggacaagcg taaccacgcg
    43141 gtagaagtga gttttctatt ggaagcgcaa aatgctgcag gtgtggagat ggtggatgaa
    43201 gacggagagg tttcatcaga tgacattctt tttgaggaag cggtcctttt ctacaatcct
    43261 gccaagtcaa ccgtcaatgc agaagactat ttaacggtta tcccttatct acctaaaaaa
    43321 ggattttctc gcgagttttt agcttatttt gcccttttcc tcaaagatac tgcggaggtt
    43381 gggctagata ccctcatgga ctttttggaa gatccagaag cagaggaatt tgttatggaa
    43441 tggaatcaag aagtctttga agaaggaaaa gttggcttgg aagagggaga attttatcct
    43501 tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac ttatttggtt
    43561 tatgtgctag ctgttgtctt tattatgcta gcttttgctt gtggaacaat cgcatttgca
    43621 gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc tcttctaagc
    43681 atgtatttaa tctttattct tcatgagctg ggtcatgctt tttgtggcta cttgacaggc
    43741 tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc aggcaggttt
    43801 catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg tttaaaagag
    43861 gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt tcatctcggc
    43921 ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt tgcagggact
    43981 tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga catcaccgat
    44041 ggagcaaaaa tttgggaatt actacaacac cctgaaaata ccaaatacgc ctacctggta
    44101 ttgagacatt ctgctcaaac tttgctggct cctcaagaat atgatttgaa agactttgtt
    44161 caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg tatgcaaggt
    44221 gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca agcattactt
    44281 gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctgct tcatatcgcc
    44341 ttgttggaag gagattttga gcaagcggag caatatgcca gtattcgaca ggtcaaatcg
    44401 tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cctggtatca attaaaggtg
    44461 aagaaagatg tagatcgaac tcgtaaggct ctcaagattg ctagacagaa aatggatagt
    44521 agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga gctggaaaag
    44581 gaattagccg aaggagtcta atatggaaat agagatttct gatttcacag gttgcaagat
    44641 tgctttgttt tgtggggata agcttttgac tatcttacgc gatgataagg caagcattcc
    44701 ctgggccaat atgtgggaac tgccaggtgg tggacgtgaa ggtgatgaaa gtccttttga
    44761 gtgtgtagcg cgtgaagttt atgaagaact gggcattcat ctgactgaag attgtctgct
    44821 ttggagtaag atttacccaa gtatgctttt tgcagacaaa cagtccgttt ttctagttgg
    44881 tcagttgaca cagaaccagt ttgacagtat cgtatttgga gatgaaggac agggctatca
    44941 gctgatgaat gttgaggaat ttcttagttc cagtcaagtt gtgcctcagt tgcaagagag
    45001 attaaaagat tatttaaaag taagtgatta gaaaggatgg ttcggttaaa tttctaaact
    45061 gaacccgccc taaacacggt gccaaaaaga taaacttctc ttagacacaa acgtcttcag
    45121 agaatttcct attttggttt tgtgttttac gggcttggta tcttaataat ggaaacatgg
    45181 caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt
    45241 gagctaggag cgcaaggtgt tgcgattgaa gacagcatgg actatgtggg aaatatggac
    45301 cgctttggcg agattttccc agaggtggag cagcaagaag aaatcgtagt gacagcctac
    45361 taccctgaca cggtggatgt gacagtggtt gaggcggact tgcaggctcg cttagcagaa
    45421 ttgacggatt ttatggattt gggagagtta aaaataggta cgactgcctt ggctgaggaa
    45481 gactgggcag acaactggaa gaaatactat gagccagctc gtatcactca tgatttgacc
    45541 atcgttccct cttggacaga ctatgaggcg actgcggggg aaatgattat caagctggat
    45601 cctggcatgg cttttggtac tggaacccac ccaactacca agatgagcct ttttgccttg
    45661 gaacaggttc ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc aggggttctc
    45721 tctattgcca gctcgcttct gggggctaaa gaaattttcg cctatgacct ggatgatgtg
    45781 gcggttcgtg tggctcagga aaatattgag ctcaaccctg gcatggaaaa tatccatgta
    45841 gcggctggag atttgcttaa gggtgtggaa attgaggcag atgtgattgt ggctaatatc
    45901 ttggcggata tcctcattca tctaatagac gatgcctatc gtttggttaa ggacgaaggc
    45961 tacctgatca tgagtggcat tatcaaggat aagtgggaca tggtgcgcga gtcggctgaa
    46021 tcagctggat ttttcctcga aactcacatg gttcaagggg aatggaatac ctgtgtcttt
    46081 aagaaaacca aggatatctc tggtgtgatt ggaggctagc atgcagcagt attttgtaaa
    46141 aggcagtgct atctctcctg tcactatcga ggacaaggaa accagtaagc atatgtttca
    46201 ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg gcatcaagcg
    46261 cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag aattaaatga
    46321 caatgtggaa ctaccagtcc aagtgaccat cgcatctgga tttcccaagg gagacaagct
    46381 ggagttcatc gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc
    46441 agactggtca gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga
    46501 aaaaattgcc cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact
    46561 tttcgagaaa aaagcagatt ttctagctca gctggaccag tttgactcta tcatagtagc
    46621 ctatgaagaa tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct
    46681 tgaaacagga tccaaactgc tctttatctt tggtccagaa ggtggtctgt cacctgcaga
    46741 aatcgaaagt tttgaagcta aaggagccgt tttggcaggc ctaggtcctc gtattttgcg
    46801 agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag aattagagaa
    46861 ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactctggg
    46921 acttaacagc cctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgtg
    46981 aagacatcaa ccaatttagc cgtgactaca agggtaatct tcatactttt gaggatttcg
    47041 agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg
    47101 cttttatgcc tcagacgacg gactatagca atgacgaatt tgccaatatt gcccaagctg
    47161 ggatggaatt tgaaacagat gccagcgtag ccttgacctt ctttgacgat gccttggtgg
    47221 tagcagacga ggaagtcttg gaccgtttgg gtgaattgcc tcacttgacg gcagctattc
    47281 gtcaggccaa aatcaaaaaa gcccactatc taggggctga tgtggagaag gccttgacca
    47341 atctcggtga agttttctat agtccgcagg acatttatac taagatgcga gctggggatt
    47401 ttgaaatggc tgactttgaa gcccatggca agacctataa aaacagcttt gtcacctatg
    47461 agaatttcta ccaaaatcac gaggatgctg aggttcgtga gaaatccttc cgttccttct
    47521 cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt
    47581 ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgactat cttctagctg
    47641 aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg aaggactttg
    47701 caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga
    47761 cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg actattgacg
    47821 atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag
    47881 ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc aaggattccg
    47941 gtggttatgc ggcggaccca tatcgcgtac acccttatgt cctcatgagc tggacaggcc
    48001 gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt
    48061 cagacaatca tcaaagttac ttcaatgccc atatgtcgac ctactatgtt gaagcaccgt
    48121 caaccttcaa tgaattgcta ctcagtgatt acttagagaa ccaatctaat gacccacgtc
    48181 aaaaacgctt tgctctggct catcgcttga cagacaccta cttccataac tttatcaccc
    48241 acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggagaaacct
    48301 tcggagcaag caaactcaac agtattatga aggaagtttt gacagatttt tggggcgatg
    48361 ccattgaaat cgacgacgat gcagctttga cttggatgcg ccaagctcac tactatatgg
    48421 gcttgtatag ttacacttac tctgctggtc ttgtcatctc gacagctggt taccttcatc
    48481 ttaaaaactc agaaactgga gctgaagact ggctcaatct cctcaaatca ggtggtagca
    48541 agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc
    48601 gtgataccat ccaattcttg tctgacacag ttgaccagat tatcgcctac agtgctgagt
    48661 tgggggagta aggggaatag aaaaattttt tagaatgatg tttatgggct tgttaagcgt
    48721 cattctattt gtcttagttg tagctatttt caaaaatgtc gttgaaggtc gtgatgttaa
    48781 atttgaactt actgtagaac tggtattgat agttatttac gtcattttat atcagattgt
    48841 atttaattga gaattcaata ttgagtcatt taagactgcc tttttgaaca gttgctagaa
    48901 atgtagaggt attgaccatg tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa
    48961 attataacca ggaggaaatc cagtggttgc ttgatcattt gggagatcct tctccagaaa
    49021 ttcgcgatga ccttgttttt acaagctttg ctatagggat tcaggaagag ctatttacac
    49081 aggagcaatt tcatttcatt gctgaagggg tttcatctga tgtaggacta gataaagaga
    49141 ttgataaggt aggtctgcca acacttgaac gttcttttag ggcacttgtt tatgcaaatc
    49201 tcttgtatgc agatgccaac cagcaatcgg ttttttatca gggattacaa ttagaaattc
    49261 gtaatgtctt ttcaaatcaa ggcttgcact atctttcaaa agaaaaggat acaacaggtt
    49321 tctcaagtca gtatggttgg gttcatgctt ttgcacatgg agccgattta ctgacagagg
    49381 tggtctgtca tccagacttt cctataaaca gaattcatga agtatttgac atacttggcc
    49441 aactatttaa aagaatgtcg attcgcttta cagatgatga ggattggcgt ttagcaagag
    49501 tgatctatga acctatttta caagggaagt tggagcaaga acaagtagct tcttggataa
    49561 aaactgttga ttttccgata gaagaaaggg aggatttttc caaattttcc aactttagat
    49621 cctgtctggt ggaagtctat gtccaacttg accagagaaa tagtttacaa gatgagttga
    49681 aagaagctat ccagtctttt caatactaga gattagctaa tagttctatg attttcaaaa
    49741 aatttccaat caattttctt gaaacccttt cctacttttg atagactagt atctagtttt
    49801 tgaaaaaagg agaaagaaaa tgaaaaaatt tgttgctgag ttaatcggta cgttcatgct
    49861 tgtgttcgtt gggacaggag ctgttgtttt tggaaatggt tttgatggcc ttggtcacct
    49921 tggaatcgcc tttgcctttg gtttggcaat cgtggtggca gcctactcaa tcggaactgt
    49981 ttcaggtgct cacttgaacc cggctgtttc gattgctatg tttgtaaaca aacgtttgtc
    50041 atcttcagaa cttgtaaact acatccttgg tcaggttgtt ggagctttca tcgcttctgg
    50101 cgctgtcttc ttcctcttgg ctaactcagg tatgtcaact gctagtcttg gtgaaaatgc
    50161 cttggcaaac ggtgtcactg tctttggtgg tttcttgttt gaagtcatcg caactttctt
    50221 gtttgtattg gttatcatga ctgtgacttc agaaagcaag ggcaatggcc cgattgctgg
    50281 tttggtaatc ggtttgtcat tgatggcgat gattcttgtc ggattgaaga ttactggact
    50341 ttcagtaaac ccagctcgta gcttggcacc agctgtcttg gtaggcggcg cagcccttca
    50401 acaagtttgg attttcatcc ttgcaccaat cgctggtgga gttcttgcag cccttgttgc
    50461 aaaaaatttc cttggaacag aagaataatt gaaactcaaa aagccttgct cctcatcttg
    50521 aggaacaggg ctttttcgta tgatactctt cgaaaatctc ttcaaaccac gtcagcttca
    50581 ccttgtcgta ggtatggtt
//
LOCUS       DAVMMR010000006        49597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMMR010000006 DAVMMR010000000
VERSION     DAVMMR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.006"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1457)
                     /locus_tag="VYS74_000326"
     CDS             complement(<1..1457)
                     /locus_tag="VYS74_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV3788413.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEEVLNQMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLG"
     gene            1595..3160
                     /locus_tag="VYS74_000327"
     CDS             1595..3160
                     /locus_tag="VYS74_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3788414.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3160..3900
                     /locus_tag="VYS74_000328"
     CDS             3160..3900
                     /locus_tag="VYS74_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3788415.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4009..4224)
                     /locus_tag="VYS74_000329"
     CDS             complement(4009..4224)
                     /locus_tag="VYS74_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788416.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5093..6343)
                     /gene="ilvA"
                     /locus_tag="VYS74_000330"
     CDS             complement(5093..6343)
                     /gene="ilvA"
                     /locus_tag="VYS74_000330"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV3788417.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6393..6749)
                     /locus_tag="VYS74_000331"
     CDS             complement(6393..6749)
                     /locus_tag="VYS74_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3788418.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(6987..7223)
                     /locus_tag="VYS74_000332"
     CDS             complement(6987..7223)
                     /locus_tag="VYS74_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788419.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7330..8352)
                     /gene="ilvC"
                     /locus_tag="VYS74_000333"
     CDS             complement(7330..8352)
                     /gene="ilvC"
                     /locus_tag="VYS74_000333"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3788420.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8418..8894)
                     /gene="ilvN"
                     /locus_tag="VYS74_000334"
     CDS             complement(8418..8894)
                     /gene="ilvN"
                     /locus_tag="VYS74_000334"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV3788421.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(8887..10587)
                     /locus_tag="VYS74_000335"
     CDS             complement(8887..10587)
                     /locus_tag="VYS74_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV3788422.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(10787..12454)
                     /locus_tag="VYS74_000336"
     CDS             complement(10787..12454)
                     /locus_tag="VYS74_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV3788423.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMSAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12457..12822)
                     /locus_tag="VYS74_000337"
     CDS             complement(12457..12822)
                     /locus_tag="VYS74_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3788424.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(12979..13167)
                     /locus_tag="VYS74_000338"
     CDS             complement(12979..13167)
                     /locus_tag="VYS74_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV3788425.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13283..13768)
                     /locus_tag="VYS74_000339"
     CDS             complement(13283..13768)
                     /locus_tag="VYS74_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV3788426.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENITQDL
                     I"
     gene            complement(14210..15754)
                     /locus_tag="VYS74_000340"
     CDS             complement(14210..15754)
                     /locus_tag="VYS74_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV3788427.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLELLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            15967..16269
                     /gene="gatC"
                     /locus_tag="VYS74_000341"
     CDS             15967..16269
                     /gene="gatC"
                     /locus_tag="VYS74_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV3788428.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            16269..17735
                     /gene="gatA"
                     /locus_tag="VYS74_000342"
     CDS             16269..17735
                     /gene="gatA"
                     /locus_tag="VYS74_000342"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV3788429.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            17735..19177
                     /gene="gatB"
                     /locus_tag="VYS74_000343"
     CDS             17735..19177
                     /gene="gatB"
                     /locus_tag="VYS74_000343"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV3788430.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19306..19866
                     /gene="efp"
                     /locus_tag="VYS74_000344"
     CDS             19306..19866
                     /gene="efp"
                     /locus_tag="VYS74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV3788431.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            19888..20277
                     /locus_tag="VYS74_000345"
     CDS             19888..20277
                     /locus_tag="VYS74_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3788432.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20270..20692
                     /gene="nusB"
                     /locus_tag="VYS74_000346"
     CDS             20270..20692
                     /gene="nusB"
                     /locus_tag="VYS74_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV3788433.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(20741..20969)
                     /locus_tag="VYS74_000347"
                     /pseudo
     CDS             complement(20741..20969)
                     /locus_tag="VYS74_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(20991..21440)
                     /locus_tag="VYS74_000348"
     CDS             complement(20991..21440)
                     /locus_tag="VYS74_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788434.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIELYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKSWNWSFVFSLFATYVVYQILGNFWARYAHLINHRNIH
                     DEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(21458..21642)
                     /gene="briC"
                     /locus_tag="VYS74_000349"
                     /pseudo
     CDS             complement(21458..21642)
                     /gene="briC"
                     /locus_tag="VYS74_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
     gene            complement(21800..22567)
                     /locus_tag="VYS74_000350"
     CDS             complement(21800..22567)
                     /locus_tag="VYS74_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV3788435.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKSLEELLEERYQRFRKY"
     gene            complement(22564..23430)
                     /locus_tag="VYS74_000351"
     CDS             complement(22564..23430)
                     /locus_tag="VYS74_000351"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV3788436.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23467..24834)
                     /gene="accC"
                     /locus_tag="VYS74_000352"
     CDS             complement(23467..24834)
                     /gene="accC"
                     /locus_tag="VYS74_000352"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV3788437.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(24846..25268)
                     /gene="fabZ"
                     /locus_tag="VYS74_000353"
     CDS             complement(24846..25268)
                     /gene="fabZ"
                     /locus_tag="VYS74_000353"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV3788438.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(25265..25750)
                     /locus_tag="VYS74_000354"
     CDS             complement(25265..25750)
                     /locus_tag="VYS74_000354"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV3788439.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(25753..26988)
                     /gene="fabF"
                     /locus_tag="VYS74_000355"
     CDS             complement(25753..26988)
                     /gene="fabF"
                     /locus_tag="VYS74_000355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV3788440.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27010..27741)
                     /gene="fabG"
                     /locus_tag="VYS74_000356"
     CDS             complement(27010..27741)
                     /gene="fabG"
                     /locus_tag="VYS74_000356"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV3788441.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFVDAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(27775..28695)
                     /gene="fabD"
                     /locus_tag="VYS74_000357"
     CDS             complement(27775..28695)
                     /gene="fabD"
                     /locus_tag="VYS74_000357"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV3788442.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(28688..29662)
                     /gene="fabK"
                     /locus_tag="VYS74_000358"
     CDS             complement(28688..29662)
                     /gene="fabK"
                     /locus_tag="VYS74_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV3788443.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(29781..30005)
                     /locus_tag="VYS74_000359"
     CDS             complement(29781..30005)
                     /locus_tag="VYS74_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3788444.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30065..31039)
                     /locus_tag="VYS74_000360"
     CDS             complement(30065..31039)
                     /locus_tag="VYS74_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV3788445.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31039..31473)
                     /locus_tag="VYS74_000361"
     CDS             complement(31039..31473)
                     /locus_tag="VYS74_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3788446.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31568..32353)
                     /locus_tag="VYS74_000362"
     CDS             complement(31568..32353)
                     /locus_tag="VYS74_000362"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV3788447.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            32678..34042
                     /locus_tag="VYS74_000363"
     CDS             32678..34042
                     /locus_tag="VYS74_000363"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV3788448.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKTKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGK
                     SKED"
     gene            34045..34416
                     /locus_tag="VYS74_000364"
     CDS             34045..34416
                     /locus_tag="VYS74_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV3788449.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34629..35903
                     /gene="serS"
                     /locus_tag="VYS74_000365"
     CDS             34629..35903
                     /gene="serS"
                     /locus_tag="VYS74_000365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV3788450.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYIGGAEVIKP"
     gene            complement(35929..36828)
                     /locus_tag="VYS74_000366"
     CDS             complement(35929..36828)
                     /locus_tag="VYS74_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV3788451.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(36937..37485)
                     /locus_tag="VYS74_000367"
     CDS             complement(36937..37485)
                     /locus_tag="VYS74_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV3788452.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(37703..39025)
                     /locus_tag="VYS74_000368"
     CDS             complement(37703..39025)
                     /locus_tag="VYS74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV3788453.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVVLLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39116..39204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39408..40970)
                     /locus_tag="VYS74_000369"
     CDS             complement(39408..40970)
                     /locus_tag="VYS74_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788454.1"
                     /translation="MKKFLLATTLLLTLVLGGCQYLPWNKSAETPSDSTSETSVATPE
                     IDYSSYNKVLDDYEKVAKGTLPTGMDVNPLASQVKSSQGFYTDVVYHKTDLNNDGVDE
                     LLLALEMKSGEKSLLDIRTLKDEKVIRLTNQENRLDQIGERMTVGILPDNSLLYRGAG
                     TATSHIYAHYQFSEDGQSLVKDKEAQELADLGVGSPISLETLSWKSVSDKLPGGSSAD
                     KGKPSVDYSPYNSILKDYEFILDYKSEVKDNINPTANIYAGMAERSQAFTYAMVDMDK
                     NGIDELIVSSKNGSEILDIFTLKDQKVIRLTNAENKMQMLGTRFKAYLLENGNLYTQW
                     NPFAGDPQTIHTIWKLNSSGDKLEKVKEAKTEDEVSPGTRMNLSSLTWKSVTEKFAAT
                     ASSTSKTASGKMDINAIKNGDFSSIEGRWSNGHGGEDLVFSKDGLIAPEKFKVDLTES
                     EFVNGFLRARYSAPDFGIFIQFVPAGVRIPDGTSRGELVTDASDISKDRIIFHAAFPL
                     HGDPRQFLYRVE"
     gene            complement(41111..42127)
                     /locus_tag="VYS74_000370"
     CDS             complement(41111..42127)
                     /locus_tag="VYS74_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV3788455.1"
                     /translation="MNKKKLLVCAVALSTVGVVSTIDTAGVLPFSAQTVHAEITRTEN
                     HEVKSKITPGSENGSIYVDMTVENVPVHYHVYIYADIVNAKTGQIPRKDTFNERFDRS
                     SVPITKQGWTNFANVPDGEYYLQFHGEDDYRSVLYKFIGKSATFTVKDQKYVNPLDSS
                     TPKETEKPTATETSKPATNSSSGQTGWSGSSYYKAGVKVVNQWIFDVQYNSYFYLNAS
                     GNFVQNAWSGSYYLKSGGYMAKSEWIYDNNYQSYYYLTAEGSYARNAWSGSYYLKSDG
                     KMAKSEWIYDSSYQSYYYLTSEGSYARNTWVGDYYLKSDGKMAVNERTPDGYKVDGSG
                     KWVR"
     gene            complement(42186..43574)
                     /locus_tag="VYS74_000371"
     CDS             complement(42186..43574)
                     /locus_tag="VYS74_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3788456.1"
                     /translation="MKSKTVLVTSLAAVTFGLVSLVDTAGALPFSAKTVQADTQTLTT
                     AAKYYILVPKDSEVFKNWRGTANRPIIWATKTTPELSYFQESARNYIANENKSGANYI
                     EWKGTVEEFKEAIKNLTDKKLTPNPAPIPQSDKKPTPDPTPNPQSTVPSGWSGSSYYQ
                     NGTKVTSKWIFDKNYNSYFYLDASGNYVQNAWEGDYYLKSGGYMAKKEWIYDKNYGAY
                     YYLTPDGYYARNTWVGNYYLKSNGKMAKSEWIYDKNYSSYYYLTSEGSYARNTWVGDY
                     YLKSNGKMAKGEWIYDNNYSSYYYLTSEGSYARNTWVGDYYLKSNGKMAVSERTPDGY
                     QVDGSGKWISSEGTHTASSSQATSLGISLYNVNDAMTYIDIALPSTPSSSYQVAYDFA
                     LVDANTGSVSTQTQFTVTYNPSLRVFQTRHFFSATPDGSYRFVIKGKGQDGKVYDGQS
                     VVFRVKNHRFVG"
     gene            complement(43750..44526)
                     /locus_tag="VYS74_000372"
     CDS             complement(43750..44526)
                     /locus_tag="VYS74_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788457.1"
                     /translation="MRTNILKLSQFLNIFYLKIALLIFSCLYFIAPWFIVLKDFETYK
                     VQYPAVIGIAEFIVLTSFLVFVYISPIFFVIFLYKIIVGSINVRRCLIKIKNYKFYMI
                     VLNRIPLHTFMVYFSSVLLWIMYLTKPVDVSNALPLPIILPLEKINPFYMILGYWLLN
                     KRCEYQLHQYLYPVMRGSSHDGRIYALPEELFIWNRNRSQKIGKWKRDRRYNTTTDLV
                     EAQIGYSKFPFFLLATKWSVDPYDQNWTIGEKAYLTSRWK"
     gene            complement(44797..45609)
                     /locus_tag="VYS74_000373"
     CDS             complement(44797..45609)
                     /locus_tag="VYS74_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEV3788458.1"
                     /translation="MLRFFKHRRNILYFILGFLWGRRQNAKVSPEPPTPSTPKHSELP
                     SISKATHNGKMTGFEPQKLKTYQLYQHELMFGEPGKGLNKSGFDESAVNLGQEGEINF
                     AKALQKKGLLEKLVTFWSVHNLNLEDERVDADIDCVIVSGSTIWLVDLKFYASGNVIY
                     READGLLYTIDSATGAQIGRPKKMSPNMSYAEESFSHKFASLLKYYRLETRVVLMPTY
                     KGAGRLDNVFWPGHIKAVSLEEMLDELSREDKFRDTIGGQMIRQTFNLLLKR"
     gene            complement(45634..46092)
                     /gene="grpE"
                     /locus_tag="VYS74_000374"
     CDS             complement(45634..46092)
                     /gene="grpE"
                     /locus_tag="VYS74_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV3788459.1"
                     /translation="MSEEVLHEKVSYIVDKVEDIESLITRRLLEDKAKNSLIEELKQY
                     LIYRQDLDKGEKFAPFMKQILQVIDRIESSEEKSDLLTSIAEELLQILSLNGLQVIDN
                     SGMIDPSMHEVVNTVAVTDEQSENNIVEVLQKGYLLNNRVLRPSKVTIAK"
     gene            complement(46105..47463)
                     /locus_tag="VYS74_000375"
                     /pseudo
     CDS             complement(46105..47463)
                     /locus_tag="VYS74_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
     gene            complement(47476..49014)
                     /locus_tag="VYS74_000376"
     CDS             complement(47476..49014)
                     /locus_tag="VYS74_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV3788460.1"
                     /translation="MSKAIGIDLGTTYSAVSVLSETGQPQILLNQDGENLTPSVVFFQ
                     DFDGKDEPLVGIQAKNLAASRPEAVVQYVKRQMGNPNWKFDSPSDTVYTAEEISAIIL
                     KRLKEGAENALGDKVEDVVITVPAYFDDARRTATKHAGEIAGLNVLRVLNEPTAAALA
                     YGISAEKNETVLVYDLGGGTFDVTLMKIKDGEFDVIATDGDRNLGGFDFDNALSMIIA
                     EKMEEQGAEDIYTDEHFTALLREKSENTKRGLTTVEKTNVFLDYKGKSYKIPITRVEF
                     EEATKSLMNRTEELLDDVVEESGMSWDEIDQVLLIGGSTRMPMVQRKLEEKIGKKIVY
                     SINPDEAVAQGAAIQAALEIANKSESADVSETVRGLAEKLVVSDVTSQALGTLALSNG
                     VKRNTIIIPKNSKVPNKYSEYFSTVVDNQQNVLVEVTQGDDEDPQFVTVIGESTIKLP
                     GDWPAGTVLKVTYHYDVDQTVFVEVHDSDNHLLGTFEVERQANLDKSAVEVASRKIKD
                     LTID"
     gene            complement(49024..49551)
                     /locus_tag="VYS74_000377"
     CDS             complement(49024..49551)
                     /locus_tag="VYS74_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788461.1"
                     /translation="MIKYKELKSYIDEWRYYSDENNPRLDLEYCKTKIVEKAKEFDLP
                     CQIDEEQIKLGGLFNKEIEECLVISHPNHQKDYVKFCFRLKNQGSVQLLTIDTLGESK
                     QLKKYYISEDNKRFREAIRESDLSLGQKLGAQLSNLTVSGLRTLGKNQSKIDAELQYC
                     EFLTEVLTQFRSNND"
BASE COUNT        14689 a        11128 c         8836 g        14944 t
ORIGIN      
        1 aaccctagaa aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg
       61 tacaagaaca aaccaaatcc gataaatagg ggcaagatag tcaagacacc catacccact
      121 tgcaaagcct tttccaaacc agttaaagga cgatagtaat ctgggagtcc ccagaagaca
      181 actcgctttc tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagc
      241 tgaatccctc ttgcttgaat ccgtttctca tcagcagctt tagctccgcg ataaaggctg
      301 tcagatacct tgtaatccgc aaacaaattg gaaagagtgg cttctttttt gcctgaaaag
      361 gctagattta gacagtctct ctcaaatctt gacaaaccat cttctttcac caatctcaag
      421 ccaactgcat ctccttctga aataatagaa acattcccac ggtccatcac atctagcaag
      481 gtagcttgaa taagttggtc aaaagtgaat ttgccagccc ccttgactaa gggactcact
      541 tcctccaagg aggtcgagta gacagcctct gataaaacca taggctctaa ttccattggt
      601 ggttcataga gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa
      661 ataaaataga agcagacact caataacaag gagatagaaa ggatcgaagg gaggacccaa
      721 gtaacaagtt gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac
      781 tcttctaaac gattcccttt caatccctga tccctagcgc tagcaaaatc ggtccgaggc
      841 cagtaggcat gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa
      901 tcaaggttac tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt
      961 ttttcagccc ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta
     1021 tctgtcagag gttgccaatt taattcagca atatcatcat aaaggaaaag taaatttttt
     1081 aagttccaga cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc
     1141 acagtataac catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg
     1201 tttttcgcgg cctgaacctt tggatgaggg tcaatgtcaa acccgctagg catcttacca
     1261 gcacgtccaa gtcccacgat ttgtccctta aagtcctcct caaactggta aactatcttc
     1321 tgtctaaact ctgccgtatt atctgcatga atatacaaat ccccttgata agagtttatc
     1381 ttgaaatcaa tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag
     1441 aaaaaagttt ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt
     1501 ttcgtaagta aaggcttact tatgttgaaa aattacgcat ttttcgataa aataggagac
     1561 agttattttt taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt
     1621 cacaagtctc ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg
     1681 catcgggatg gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg
     1741 agctgtcaaa atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa
     1801 gaaaatcgct aaggacttag gtaaagaacc tttggttgtt aaaaccaagt gggaaggtct
     1861 agtccctgcc cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc
     1921 agaacgcaaa caagaaattg ccttttcgag cagttactac actagcgaac cagttttgct
     1981 tgtcaaaaaa gattctgcct acgcaagtgc taaatctttg gatgacttta atggtgcgaa
     2041 aatcacttct caacaaggtg tctacctcta cgacttgatt gcacaaatcc caggtgctaa
     2101 aaaagaaaca gccatgggag acttcgctca aatgcgccaa gctcttgagg ctggtgtcat
     2161 tgatgcttat gtttctgaac gtccagaagc actgactgct gaagctgcga actctaagtt
     2221 caagatgatt caagtagaac ctggtttcaa aactggggaa gaagatacag ctatcgctat
     2281 cgggcttcgt aaaaatgaca atcgtattag ccaaatcaat gccagcattg aaaccatttc
     2341 aaaagatgac caagttgcct tgatggatcg tatgatcaag gaacaacctg ccgaagctac
     2401 aacaactgaa gagactagca gtagtttctt tagccaagtc gctaaaattc tttctgaaaa
     2461 ctggcaacaa ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat
     2521 cataggtctc attattggac ttgccattgg tgtcttccgt actgctcctc tctctgaaaa
     2581 caaagccatt tatggccttc aaaaactagt cggctgggtt ctcaatgtct acattgaaat
     2641 tttccgtggt acgccaatga ttgttcaatc gatggttatc tactatggaa ctgcccaagc
     2701 tttcgggatc aaccttgacc gtacactggc tgctatcttc atcgtttcaa tcaataccgg
     2761 tgcctacatg actgaaatcg tccgtggtgg tatcctagca gttgacaagg gacaatttga
     2821 agctgcgact gctcttggta tgacccataa ccagaccatg cgtaagattg tcctacctca
     2881 ggtagtccgt aacatcctac ctgcaactgg taatgaattt gtcatcaata tcaaagatac
     2941 atctgtattg aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac
     3001 acaaacctat caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac
     3061 cttcaccgta acacgtatcc tacgatttat cgagcgccga atggacatgg atacctatac
     3121 tacaggtgct aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa
     3181 attaaacacc tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttcactcact
     3241 gtccacaagg gagaggtcat ctctatcatc ggaagctctg gaagcggaaa atcgaccttc
     3301 ctacgctcca ttaacctact tgaaacacca actgatggac aaatccttta tcatggacaa
     3361 aacgtcctcg aaaaaggcta tgacctcacg caataccgtg aaaagttggg gatggttttc
     3421 caatccttta acctctttga aaatctcaat gttcttgaaa acacaatcgt cgctcagaca
     3481 actgtcctaa aacgcgaacg cacagaagct gaaaagattg ccaaagaaaa cctggaaaag
     3541 gtcggcatgg gagaacgcta ctggcaagcc aaaccaaaac aactctcagg tggtcaaaaa
     3601 caacgtgtgg ccatcgctcg tgccctctcc atgaatccgg acgctattct ctttgatgaa
     3661 ccaacatcag ctctcgatcc agaaatggtt ggagaagtcc tcaaaatcat gcaggacctg
     3721 gctcaggaag gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc
     3781 tctcaccgtg ttatctttat ggataagggc gtgatcgctg aagaaggtaa accagaagac
     3841 ctcttcacca atcctaaaga agaccgaaca aaagagttcc ttcaacgcta tctcaaataa
     3901 aaagaaaagg ctgcatcaac cgtgcagtct ttttgctgtc ctcaaaatga aaaggcagat
     3961 tctctatctc tactacttga aggtcaagaa tccgccgtta tccaaagatt aatcttcaaa
     4021 tttttcataa tttttttcat agaaatcaat taagcctagg gctgtttcaa atgaaatatt
     4081 ttttactttt gcacgaccct gcgccagagc aatgatagac atttcacgcg cattcgtttc
     4141 tttagagata cggtagcctg tgattttctt atcacgaacc cagccaacaa ctgattctac
     4201 tttttcaaag tttgacttag ccatgttttt ctcctatttt cttctttata atatttaatt
     4261 gtatacgttt ttactacttt tgtcaataaa aaaaacatat attcaacgaa aataattagt
     4321 tcagaatttt cttacttcct ttttaaatcc gataatatat aggttttgct agcctctaca
     4381 ccataaatat ttgctaataa atactgtatt attaccctct taaggtgcga aattattcaa
     4441 gttcttaaaa aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg
     4501 aagatgatag ttctaacctt tagaaatcca agccttcact agctcaaaaa ctcctatctt
     4561 tcttcctcaa taaatcatct gagagatagg tatttcttat atccttcaaa ttcatcactc
     4621 ctttggatat tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat
     4681 agagatgaca agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat
     4741 ttgtcaacaa aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta
     4801 ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga gctaacgtgg
     4861 tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca atagatagga
     4921 aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt
     4981 aagaattgca cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt
     5041 atagcatata tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa
     5101 gcatattata aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca
     5161 ttctacgaat caaacctgca taatcatgct tatctgctaa agcgatccca atcaatactg
     5221 ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc
     5281 ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa
     5341 aataatgttt gataccatca taaatcaagg cacgctcttc catttctggc atacggttga
     5401 tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta
     5461 aaacctctaa agaggcgata ctagctgctc cagcaggttc tgcgactatc ccttgcttag
     5521 agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagtttgaa
     5581 tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca atcccatccg
     5641 caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg
     5701 aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat
     5761 aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat
     5821 caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt
     5881 gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt
     5941 gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc
     6001 caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaatgggc ataaagatag
     6061 tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag
     6121 aagcgcaaac taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg
     6181 caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag
     6241 caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt
     6301 tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct
     6361 ataaaaaaca gtcactaatg actgtttttt agttattcat ctccgaaaat ttcagtaaca
     6421 ctttcatggt atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt
     6481 gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag
     6541 ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat
     6601 tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat
     6661 tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc
     6721 ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa
     6781 tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt
     6841 caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat
     6901 tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg
     6961 tcctaatgat ttaccaacta tatttgttaa tattttttat ttctcaaaat ccatttcgct
     7021 atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct
     7081 tcttctctag ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca
     7141 tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct
     7201 gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac
     7261 caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata
     7321 tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat
     7381 tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta
     7441 agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc
     7501 attttggatg tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg
     7561 tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt
     7621 gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta
     7681 agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc
     7741 agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt
     7801 gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc
     7861 aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa
     7921 tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac
     7981 atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc
     8041 aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat
     8101 ttcgtctggc gccaagatca tgataacatc agccaactta gtagcttctg ctactgtgta
     8161 agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat
     8221 aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc
     8281 gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat
     8341 ttgaactgtc atagcttttc tctcttttct attatttatt gccttttaag ctggtttaac
     8401 aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg
     8461 cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc
     8521 atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa aggttgaata
     8581 atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg
     8641 cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg
     8701 agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata
     8761 cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga
     8821 cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt
     8881 aacattctac gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac
     8941 cattggtaac acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc
     9001 agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat
     9061 accataagcc tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt
     9121 tctgccttca tagaaggatt cctgccactg gcgaaccatt ccgagtgaat gattgttcag
     9181 cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat
     9241 ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc
     9301 accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg
     9361 acgttcattt tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt
     9421 taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg
     9481 aaccacacgc tctttcttgt catacgaacg aacacgattc ttgtctttag tgactttctc
     9541 aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt
     9601 cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc
     9661 aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg
     9721 gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg caatatttgc
     9781 tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt
     9841 tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag
     9901 ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc
     9961 cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg
    10021 ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc
    10081 ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc
    10141 cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg
    10201 catggtaatt cctacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc
    10261 cacctgacct gtaaagacca aaaggggaac gctatcgctc atagcatccg caatccctgt
    10321 aatggcattt gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga
    10381 tttggcatag ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg
    10441 aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc
    10501 aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt
    10561 aggagattct aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg
    10621 ttactatcat accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt
    10681 ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca
    10741 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
    10801 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
    10861 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcaccgacat
    10921 agaaggttac gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt
    10981 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt
    11041 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
    11101 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
    11161 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg
    11221 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
    11281 agaagatgat gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag
    11341 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
    11401 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
    11461 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
    11521 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
    11581 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tctccaagct
    11641 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
    11701 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac
    11761 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
    11821 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
    11881 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
    11941 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgctcatg acttcaacag
    12001 tgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtca
    12061 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
    12121 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
    12181 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
    12241 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
    12301 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
    12361 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
    12421 tttcttgaaa taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt
    12481 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
    12541 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacagt
    12601 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
    12661 attttcttta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
    12721 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
    12781 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
    12841 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaataat gtatcagcgg
    12901 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
    12961 tctcctttgc ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga
    13021 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
    13081 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
    13141 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
    13201 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
    13261 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gatattttct gctctccaca
    13321 tgccattgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
    13381 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
    13441 caacaatatc tgaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
    13501 acctaaaaaa agccgcatat gaaagtccct caaaggggag acgataacct ttcagaatac
    13561 tactgtcaat ctgaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
    13621 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
    13681 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
    13741 agacaatcgc ttttttctct tctttcactc tttatcttat ttctttttat tcccatcata
    13801 ttattcccat catatgtttc tacgtaacca ttattttcgc ctattcgttc gtaaaaccat
    13861 accagtggag attttagatg aagtcccatt acggtttaca atttttacat tacgacacgg
    13921 agttttacaa atcgatttca tttgccaaac gtagttagta aggcagttag ctaggctacc
    13981 tactttctgc tacgacggaa aaatccacaa atcaatacca ttcgtggatt tttcctagtg
    14041 aagcgtttag gtaagccgcc atagcggtta ccgattatta tattacgaca cggagtttgg
    14101 caaatcgatt ctatttgcca aacgtagtta gtgaggcagt tagctagttc gccaaatagc
    14161 gactagcgtc caacaatttg gaactttagt tccaattgtt ggtactgagt cacatcttct
    14221 cctctaactc tacgtctgga tacttgtccg caaaccagcg gagggcaaag tcattttcaa
    14281 agagaaagac tggttggtca aaacggtctt tggctaagat attgcgactt gacgacatcc
    14341 gttcatccaa gtcctcaggc ttgatccaac gaacggtctt tttacccatt gggttcataa
    14401 ctacttctgc attgtactcg ccttccatgc ggtgtttaaa gacttcaaac tggagttgac
    14461 ctacagcgcc tagcatgtac tcacctgttt ggtaattctt ataaagctga acggctcctt
    14521 cttgcaccaa ttgctcaatc cccttgtgga aggatttttg cttcataaca ttcttagcag
    14581 aaactttcat gaaaatttca ggagtaaagg ttggcagtgg ttcaaattca aacttgtttt
    14641 taccaactgt caaggtatcc ccaacttgat aagtaccggt atcgtaaacc ccgataatat
    14701 cacctgccac ggcattgatc acattctcac gactttccgc cataaactga gtaacattag
    14761 acagtttagc ccccttacca gtacgaggga gattgacact catgccgcgc tcaaattcgc
    14821 cagatacgat acggacaaag gcaatacggt cacggtgacg agggtccatg ttggcttgga
    14881 ttttaaagac aaatcctgag aaatccttgt cataaggatc cacaatttca ccgtctgttt
    14941 tcttgtgacc atgtggttct ggagcaaact tgaggaaggt ttcaaggaag gtctgcacac
    15001 caaagtttgt cagggctgaa ccgaaaaaga caggcgtcaa ttctccagcc agaatagctt
    15061 cctctgaaaa ctcattcccg gcttcattta aaagctcaat gtcatccttg acttgctcgt
    15121 agaaaggatt gctaccaaag agtttgtccc cgtcttctag actggcaaaa cgctcatccc
    15181 ctttataaag ttctaaacgt tggttataga ggtcatacaa gccctcaaag gctttcccca
    15241 tcccgatagg ccagttcata gggtagctag caatgcccaa gatttcttcc aattcttgca
    15301 agagttccaa aggctcacga ccgtcacggt ccagcttgtt cataaaggta aagactggaa
    15361 tgccccgatg tttcacaacc tcaaacaatt tcttggtttg agcctcgatc cccttggcag
    15421 agtccacgac catgaccgca gcatccaccg ccatcaaggt acgataggta tcttctgaga
    15481 agtcctcgtg ccctggcgtg tctaagatat tcacgcgctt gccgtcgtag tcaaattgca
    15541 taacagatga agtaacagaa atcccacgtt gcttctcgat atccatccag tcagatttag
    15601 caaaagtccc tgttttcttc ccttttaccg taccagcctc acgaatctca cccccaaagt
    15661 agagtaactg ctcagtgatg gttgttttcc ccgcgtccgg gtgggagata atggcaaagg
    15721 tacgacgttt cttaatttct tcttgaatat tcataagttc tctttctttg attctctatt
    15781 tttcttgttt caatagctga gaatgatttt tacattggat tttaccattc ctttcaacac
    15841 tccattatat cggattttag catttttttc aatttctatt tcttttcact tccccctccc
    15901 ttatttatag gaaaatatgg taaaatagaa cagactaaaa atcatcattt cacgaaagga
    15961 tgcaagatga aaattacgca agaagaggta acacacgttg ccaatctttc aaaattaaga
    16021 ttctctgaaa aagaaactgc tgcctttgcg accaccttgt ctaagattgt tgacatggtt
    16081 gaattgctgg gcgaagttga cacaactggt gtcgcaccta ctacgactat ggctgaccgc
    16141 aagactgtac tccgccctga tgtggccgaa gaaggaacag accgtgatcg cttgtttaaa
    16201 aacgtacctg aaaaagacaa ctactatatc aaggtgccag ctatcctaga cgatggagga
    16261 gatgcctaat gacttttaac aataaaacta ttgaagagtt gcacaatctc cttgtctcta
    16321 aggaaatttc tgcaacagaa ttgacccaag caacacttga aaatatcaag tctcgtgagg
    16381 aagccctcaa ttcatttgtc accatcgctg aggagcaagc tcttgttcaa gctaaagcca
    16441 ttgatgaagc tggaattgat gctgacaatg tcctttcagg aattccactt gctgttaagg
    16501 ataacatctc tacagacggt attctcacaa ctgctgcctc aaaaatgctc tacaactatg
    16561 agccaatctt tgatgcgaca gctgttgcca atgcaaaaac caagggcatg attgtcgttg
    16621 gaaagaccaa catggacgaa tttgctatgg gtggttcagg tgaaacttca cactacggag
    16681 caactaaaaa cgcttgggac cacagcaagg ttcctggtgg gtcatcaagt ggttctgccg
    16741 cagctgtagc ctcaggacaa gttcgcttgt cacttggttc tgatactggt ggttccatcc
    16801 gccaacctgc tgccttcaac ggaatcgttg gtctcaaacc aacctacgga acagtttcac
    16861 gtttcggtct cattgccttt ggtagctcat tagaccagat tggacctttt gctcctactg
    16921 ttaaggaaaa tgccctcttg ctcaacgcta ttgccagcga agatgctaaa gactctactt
    16981 ctgctcctgt ccgcatcgcc gactttactt caaaaatcgg ccaagacatc aagggtatga
    17041 aaatcgcttt gcctaaggaa tacctcggtg aaggaattaa cccagaggtt aaggaaacca
    17101 ttctaaatgc cgctaaacac tttgaaaaat tgggtgctat tgtcgaagaa gtcagccttc
    17161 ctcactctaa atacggagtt gccgtatact acatcatcgc ttcatcagaa gcttcatcaa
    17221 acttgcaacg ctttgacggt atccgttacg gctatcgcgc agaagatgca accaaccttg
    17281 atgaaatcta tgtaaacagc cgaagccaag gttttggtga agaagtgaag cgccgtatca
    17341 tgctgggtac tttcagtctt tcatcaggtt actacgatgc ctactataag aaggctggac
    17401 aggtccgtac acttatcatt caagatttcg aaaaagtctt cgcggattac gatttgattt
    17461 tgggtccaac tgctccaagt gttgcctatg acttggattc tctcaaccat gacccagttg
    17521 ccatgtactt agccgaccta ttgaccatac ctgtaaactt ggcaggactg cctggaattt
    17581 cgattcctgc tggattctct caaggtctac ctgtcggact ccaattgatt ggtcccaagt
    17641 actctgagga aaccatttac caagctgctg ctgcttttga agcaacaaca gactaccaca
    17701 aacaacaacc cgtgattttt ggaggtgaca actaatgaac tttgaaacag tcatcggact
    17761 tgaagtccac gtagagctca acaccaattc aaaaatcttc tcacctactt ctgcccactt
    17821 tggaaatgac caaaatgcca acactaacgt gattgactgg tctttcccag gagttctacc
    17881 agttctcaat aaaggggttg ttgatgccgg tatcaaggct gctcttgccc tcaacatgga
    17941 catccacaaa aagatgcact ttgaccgcaa gaactacttc tatcctgata accccaaagc
    18001 ctaccaaatt tctcaatttg atgaaccaat cggatataat ggctggattg aagtcaaact
    18061 agaagacggt acgaccaaga aaatcggtat cgaacgtgcc cacctagagg aagacgctgg
    18121 taaaaacacc catggtacag atggctactc ttatgttgac ctcaaccgcc aaggggttcc
    18181 cttgattgag attgtatctg aggcagatat gcgttcgcca gaggaggcct atgcttatct
    18241 gacagccctc aaggaagtta tccagtacgc tggcatttct gacgttaaga tggaagaagg
    18301 ttctatgcgt gtggatgcca acatctccct tcgcccttat ggtcaagaaa aattcggtac
    18361 caagactgaa ttgaagaacc tcaactcctt ctcaaacgtt cgtaaaggtc ttgaatacga
    18421 agtccaacgc caggctgaaa ttcttcgctc aggtggtcaa atccgccaag aaacacgccg
    18481 ttacgatgaa gcgaataaag caaccattct catgcgtgtc aaggaagggg ctgcagacta
    18541 ccgctacttc ccagaaccag acctacccct ctttgaaatt tctgacgagt ggattgagga
    18601 aatgcggact gagttgccag agtttccaaa agaacgtcgt gcgcgttatg tatctgacct
    18661 tggtttatca gactacgatg ctagtcagtt gactgctaat aaagtcactt ctgacttctt
    18721 tgaaaaagct gttgccctag gtggtgatgc caaacaagtc tctaactggc tccaagggga
    18781 agtcgctcag ttcttgaatg ctgaaggtaa aacactggaa caaatcgaat tgacaccaga
    18841 aaacttggtt gaaatgatta ccatcatcga agacggtact atttcatcta agattgccaa
    18901 gaaagtcttt gtccatctag ctaaaaatgg cggtggcgcg cgtgaatacg tggaaaaagc
    18961 aggtatggtt caaatttcag atccagctat cttgatccca atcatccacc aagtctttgc
    19021 cgataacgaa gctgctgttg ccgacttcaa gtcaggcaaa cgtaacgccg acaaagcctt
    19081 tacaggattc cttatgaagg caaccaaagg ccaagccaac ccacaagttg cccttaaact
    19141 acttgcacag gaattggcga agttgaaaga aaactagaca gaacaaaacc agccctaagg
    19201 ttggtttttt cttctctacc aactcccaat aactattttg gctttatttc cagagtattt
    19261 tatggtaaaa tgaagagtaa taatatttat taaagaggta aaaacatgat tgaagcaagt
    19321 aaattaaaag ctggtatgac ctttgaaaca gctgacggca aattgattcg cgttttggaa
    19381 gctagtcacc acaaaccagg taaaggaaac acgatcatgc gtatgaaatt gcgtgatgtc
    19441 cgtactggtt ctacatttga cacaagctac cgtccagagg aaaaatttga acaagctatt
    19501 atcgagactg tcccagctca atacttgtac aaaatggatg acacagcata cttcatgaat
    19561 acagaaactt acgaccagta cgaaatccct gtagtcaatg ttgaaaacga attgctttac
    19621 atccttgaaa actctgatgt gaaaatccaa ttctacggaa ctgaagtgat cggtgtcacc
    19681 gttcctacta ctgttgagtt gacagttgct gaaactcaac catctatcaa aggtgctact
    19741 gttacaggtt ctggtaaacc agcaacgatg gaaactggac ttgtcgtaaa cgttccagac
    19801 ttcatcgaag caggacaaaa actcgttatc aacactgcag aaggaactta cgtttctcgt
    19861 gcctaatctc tagaaagagg tcattctatg ggaattgaag aacaacttgg cgaaatcgtt
    19921 atcgccccac gtgtacttga aaaaatcatt gctatcgcta ctgcaaaggt agagggtgtt
    19981 cactcttttt caaacagatc agtgtctgat accctttcaa aactttcact cggccgtggc
    20041 gtttatctta aaaacgtgga cgaagaactc acagcagata tctatctcta ccttgagtac
    20101 ggagtaaaag ttcctaaggt agcggttgct atccagaaag ctgtcaaaga tgccgtccgt
    20161 aatatggctg atgtagaact cgctgctatc aatattcacg ttgcaggtat cgtcccagat
    20221 aaaacaccaa aaccagaatt gaaagatcta tttgacgagg acttcctcaa tgactagtcc
    20281 actattagaa tctagacgcc aactccgtaa atgcgctttt caagctctca tgagccttga
    20341 gttcggtacg gatgtcgaaa ctgcttgtcg tttcgcctat actcatgatc gtgaagatac
    20401 ggatgtacaa cttccagcct ttttgataga cctcgtttct ggtgttcaag ctaaaaagga
    20461 agaactagat aagcaaatca ctcagcatct aaaagcaggt tggaccattg aacgcttaac
    20521 gctcgtggag agaaacctcc ttcgcttggg agtctttgaa atcacttcat ttgacactcc
    20581 tcagctggtt gctgttaatg aagctatcga gcttgcaaag gacttctccg atcaaaaatc
    20641 tgcccgtttt atcaatggac tgctcagcca gtttgtaaca gaagaacaat aaaaagaaaa
    20701 agtgtttgat agatatcaaa cactttttct ttgcctccca ctatctaaaa aaatgataat
    20761 agatataatt gtaaacaaaa atccagatag gttttgcatg attgagaaag ttaaaaaaaa
    20821 ctatggcaga gaatcgttaa tctcagattg tcggtaaaac gataaacaag ggcaaaaaag
    20881 aaaccaatca gactataata taataaacta attggatctc tgtgagatag tatcaaatga
    20941 ctaagcccaa agataaggcc tgataagatg acatctaaat aatacttgga ctagggaaag
    21001 aaggtattca taaaataccc tctatcaaga gtctcctcaa aaacaggacc gatgattaca
    21061 ggcaggacaa aagataagat agtcgataaa aaggttggtt gtccatttga aagaaccacg
    21121 gtaaaatact catcatgaat attcctatga ttaatcaaat gagcatagcg tgcccaaaaa
    21181 ttaccgagaa tctgataaac cacataagtt gcaaataagg agaagacaaa tgaccagttc
    21241 cagctctttt tcttttttaa cctccaaatt aatagaagga aacttcccac taataccatt
    21301 gttaaaataa gagaatagac atcagctcct aaccctaaaa tgatcgtcac atacaattca
    21361 attgtttgtg gtaaataggt agatagtaaa ataataagca aaaatattcc aaattgtctt
    21421 agtttctttg tgtttctcat cgtacttttt tgaaagatta ccctgttcgg aagccgtact
    21481 tccaagcatc tatataagaa ttaagtgccc ttgcctcata tagggagcaa attctctata
    21541 atataaccat ctactatatc catcttccca aacagcaaga ccacctgaag tttgctccaa
    21601 gtcctcagtt gaaataactg taaatgtttc tgtacctgtc attgcaagta ccttcttaaa
    21661 atagattgtt gtaggctcac atttatagta tatttctttt ttgtctattt tatagcccat
    21721 ctcctcaact atcaattttt caacctgaat tgcatttttc cataaaaaat gagacctttc
    21781 tagtctcatt tagtcattct tagtattttc taaatcgttg atagcgttct tccagcaact
    21841 cttctagcga tttttgtgaa agtctagcca gctccgtttg gagttctttt ttgacactct
    21901 taatcagttc tttactagaa agtcctactt cagaaatcac cttatccacc acgtccattt
    21961 ctaacagttc atgcgaagtg attttcatca gttctgctgc ttccatggcg cgagtaccgt
    22021 ccttccataa aatggaagca aagccttctg gactgagaat ggcatagata gaattttcca
    22081 gcatccagac acggtccgcg acagctagag ccagagcccc gcctgaacca ccttcaccga
    22141 taataatggc gataatagga actttcaggt cactcatttc catgagattg cgagcgatag
    22201 cttccccttg accacgttct tccgctccga caccaggata agcacctgct gtattgataa
    22261 aggtcacaac tggacggcca aatttctcag cctgtttcat caaccgcagt gcctttcggt
    22321 agccttctgg atgtggttgg ccaaaatttc gtttgaggtt gtcttgcaaa ctcttgcctt
    22381 tttggatacc aaccactgtt acagcttggt ctccaagcca accaatacca ccaacaactg
    22441 caccatcatc acgaaaagaa cggtcaccat gtaattggat aaattcatca aaaatgcctg
    22501 tcgcaaagtc caaggttgtc aagcgactct gctcacgcgc ttctctgact atttttgcaa
    22561 tattcatcta ggactccctc catgcaatct gactaggcta gcaatcgtat ctggtaagtc
    22621 tcttcttttg acaatagcat ccacaaagcc atgttctaat aggaattctg ccttttggaa
    22681 atcctcaggc aagctttcac gaaccgtatt ttcaatcaca cgacgtccag caaaaccaac
    22741 caagctctgt ggttcagcca gaatgatatc gccttccata gcgaaagaag ctgtcacacc
    22801 accagtcgtt ggatctgtca aaatggtcag gtaaaagaga ccagcatttg aatggcgttt
    22861 aaccgccgca gagatcttag ccatctgcat gagactcatg attccttcct gcatacgggc
    22921 tccaccagag gctgtgaata ggacaactgg caatttttcg acagtcgcat actcaaacaa
    22981 acgagtgatt ttttcaccta caaccgtacc catagaagcc atgataaagt tagaatccat
    23041 aatcccaaga gccacagtct gacctttaat aagagcagtt cctgtcacaa cggcttcatg
    23101 cagacctgtt ttttcacgca tagatgccag tttcttttgg taaccaggga aatgcaaggg
    23161 atccttgctt tcaatccctg taaacaattc tttgaaggtt cccatatcaa tcgtcaaagc
    23221 caagcgttct tgggcagaaa tacgaaaggt atagctacag tgcggacaga tacgttcact
    23281 tcccagatcc ttctgataga tggtatgctt acagcctgga cactgggaaa ataattcatc
    23341 tggaacctct ggcttagctt gaggtttttc cctaaccgaa cgattgggat tgattcgaat
    23401 atacttatct tttttactaa atagagccat tgattcccct tttcggttta aactcttaaa
    23461 gtcattttat tctttttctt gatatttagg taagaaggtt tccatcaaga aggaagtatc
    23521 ataatcccca gcaatgacat tgcgatctga aatgaggtca agctggaaat ctgcattggt
    23581 ctgcactcct tcaatttcta attcatagag ggcacgttgc attttcatca aggcgtcaaa
    23641 acgattttcg ccgtgtacta tgattttggc aatcatacta tcataataag gcggaatggt
    23701 ataacctgga taaactgctg aatccacgcg caagccaact ccaccacttg gcagatagag
    23761 attagtaatc ttacctggac ttggagcaaa gttaaaggct gggttttctg cattgatacg
    23821 acactcgatg gcatgaccgc gtaggacaat atcttcttgc ttaacagaca aaggctgacc
    23881 tgccgcaatg caaatctgtt ccttaacgat atcaacacct gaaacaaact ctgttactgg
    23941 atgttctacc tgaacacgag tattcatctc catgaaatag aaattgctac ttgcttcatc
    24001 aagaagaaat tcaatggttc ctgcattctc atagccaaca aactctgccg ctcgaacagc
    24061 agcagcacct atttcatgac gcagcgtttt tccgattgca atcgagggac tttcttccaa
    24121 aaccttttgg ttattccttt gaagagaaca atcccgttca cccaagtgaa tcacatgtcc
    24181 atgctcatca cctaggattt gaacctcaat gtgccgagct ggatagataa cccgttctat
    24241 gtacatggca ccattgccat aattggcctt ggcctcacta gaggcagttt caaaggcaga
    24301 aacgaggtca tctggttttt caaccttacg aatcccttta ccacctccac ctgctgaagc
    24361 cttgagcata acaggatagc caattttttc agcaacaatc aaagcttctt cagagttatg
    24421 cacttctcca tctgaacctg gtataacagg cacacctgct ttaatcatct gagcacgcgc
    24481 attgatctta tcccccatca tatccataac atgaccagat ggaccgataa acttgatacc
    24541 tacttcttca cacatggtcg caaatttgga attttcactg agaaatccaa aaccagggtg
    24601 aatagcttct gcctcagtca agactgcagc tgatagaact gcattaatat tgagataaga
    24661 ctctgttgcc ttgccaggac caatacaaac tgcttcatct gccaacagcg tatgaagagc
    24721 ttccttatca gcagttgaat aaaccgctac cgtcgcaatc cccaattcac gtgccgcacg
    24781 gataatacga accgcaattt caccacgatt ggcaattaaa atttttcgaa acatggagaa
    24841 cctccttagt tcccaattgc aaaagtaagg gtaccactgg ctgcaagctt gccatccact
    24901 tcagcctttg cttcaaccac agctatggtg ccacgacgtt ttacaaaagt cgctgtcata
    24961 accaattggt cgcctggtac aacttgcttc ttgaacttaa ccttatccat accagcgtaa
    25021 aagaccagtt ttcctttatt ttcaggtttt gataactcca acacaccagc agtttgcgcc
    25081 aaggcttcca taatcagaac acctggcata actgggtatt gaggaaagtg gccgttaaag
    25141 aaaggctcgt tgatggtcac atttttgata gcaacaatgg tatcctcgct cacctccaag
    25201 acacggtcca ctagaagcat aggataacgg tggggaaggg cttctttgat tccttgaata
    25261 tcgatcattt gatacgtacc aatcctttac caaactcaac catttcttcg ttagagacga
    25321 gaatttccgt taccacacca tccttaggag ctgggatttc attcatgact ttcatggctt
    25381 cgataattac caatgtttga ccttttttga cactatcacc aactgtaacg aaggcaggtt
    25441 tatctggtcc agcagccaag taaaccactc caacaagtgg actctctaca agatttccct
    25501 cactagccac acttgcttca gctggagctg gaacttcttc tgctacagtc tctgctggag
    25561 cagatgtagg agctactgga ctcggtgttg ctagaacggg tgctggagcg acttgagttg
    25621 caacttcagg cacaggtctc gcttcattct tgctaaactg caactcatcc gtcccatttt
    25681 tataagaaaa ttctctcaaa cttgactggt caaattgagt catcaagtct ttaatatcgt
    25741 ttaaattcat acttatctat tctcccaacg tttgaaagca agaactgcat tgtggcctcc
    25801 aaaaccaaaa gtatttgaaa tagcgtatgg aatttctttc tccaagcctt gtccataaac
    25861 gacattagct tcgatataat ctgatacttc acttgtccca gctgtcattg gtacaaagtt
    25921 atgacgcata gcttcgatgg tgacgatagc ttctactgca cccgcagccc ccagcaaatg
    25981 tcctgtaaaa gacttggttg atgatacagg tacttcctta ccaagaacag ctacgatagc
    26041 accactttct cctttttcat tggcaggagt tgacgttccg tgagcattga cataggctac
    26101 ttgctctgga gaaatctcag cttcttccaa ggctagtttg atggccttga tagctccctg
    26161 accttctgga tgtggagaag tcatgtggta ggcatcacaa gtatttccgt aaccaaccac
    26221 ttcagccagg atagtagctc cacgtttttc agcgtgttca agactttcta gaaccaacat
    26281 ccctgaacct tcacccataa caaacccatt gcgatcctta tcaaatggga tcgaagcacg
    26341 agtcggatcc tctgtagtag agagagctgt taaggcttgg aaaccagcga tggcaaaagg
    26401 tgtgatagaa gcttctgttc ctcccaccaa catcacatct tggaaaccaa acttaatgga
    26461 gcggaaggca tccccaatcg catcatttga tgaagagcag gcagtattga tagatttaca
    26521 aacaccgttt gcaccaaaac gcatggctac attcccagaa gccatatttg gtaaagcttt
    26581 tggaagagtc attggtttga cacgtttggg tcctttttca tgaaggcgaa gtacctgatc
    26641 ttcaatttcc ttgattccac caataccaga tgcaacgata acaccaaaac gatccctatt
    26701 aagagcctct acatcaagat tggcatgatt tacagcctct tgggctgcat acaaggcata
    26761 taaagaatag ttatcaaaac ggttggtatc tttttttaca aagtatttat cgaacggaaa
    26821 atcttggatt tctgccgcat tatgcacatc aaagtcacta tgatcaaatt ttgtaatgcc
    26881 accaatgccg attttcccag ttgctaaact attccaaaat tcttctggtg tatttccgat
    26941 tggagatgtt actccataac ctgttaccac tactcgattt agtttcattc ttttcacctc
    27001 tagctttcgc tacatactta agccaccatc aatggcaacc acttgaccag ttagataatc
    27061 ttggcctgct aaaaatactg tcaaatctgc aacctgctct gcctgcccaa attctttcat
    27121 cggaatctga gctagtgtag cttccttaat cttatctgat aagatagctg tcatatcaga
    27181 ctcaatcatt cctggagcaa tcacattgac tcgtatattc cgactagcga cctcgcgtgc
    27241 cacagacttg gtaaagccaa tcaagccagc cttagaagca gcatagttag cttgaccaat
    27301 attccccatc aaaccaacaa cactagacat attaatgata gcaccttctc tggctttcat
    27361 catcggtttc aagactgatt gtgtcatatt aaaggcacca gtcagattga ccttgagcac
    27421 tttttcaaaa tctgcttctg tcatcttgag cataagagta tcttgggtaa tccctgcatt
    27481 gttgaccaaa acatctactg aacccagttc tgcaatagct tgatcaatca tacgcttagc
    27541 gtctacaaaa tctgatacat ctcctgaaat gggaaccacc ttgataccat agtttgaaaa
    27601 ctcagcgagc aattcttctg agattgcccc acgactgttt aagacaatgt tggctcctgc
    27661 ttgagcaaac ttgtgggcga tggcaagacc aattccacga ctcgaacctg taataaagat
    27721 atttttatgt tctagtttca tttttttcct ttcaaaactt ctacttattt tagtctattt
    27781 ttctaaaagt gctactaaac tcgcttgatc ttccacatga gctaagtgag cagtttgatc
    27841 aattttttta acaaaacctg acaagacttt ccccggtcca atctcgataa agttgcttat
    27901 gcctgcttct tgcatgaccc caatactttc atagaaacga acgggttcct tgacctgacg
    27961 cgtcaagagc tgagcaatgt cctctttttg catcacagca gcttctgtat tgccgactag
    28021 gggacaagta aaatctgaaa aacttacctg agctagagtt tcagctagtt tctggctagc
    28081 aggctcaagg agagcggtgt gaaagggacc tgacacctta agaggaatca agcgtttggc
    28141 acctgcttct tgcaaaagtt caaccgctcg atcaactgca accacttctc cagcaatgac
    28201 gatttgtgca ggtgtgttat agttggctgg agtaaccact ccaagttcag aagctttttg
    28261 acaggcttct tcaatgacct ctactggcgt attgagaact gctaccatct tgccagagtc
    28321 agcaggagcc gcttcttcca tataggctcc acgcttagct accaaggcaa ccgcatcttc
    28381 aaaatccaag gcgccgcttg ccaccaaggc agagtattct ccaagagaca aaccagcaac
    28441 catatcaggc tgatagccct tttcttgcaa taaacggtag atagcaaccg aagtcgctag
    28501 aatggctggt tgcgtatagc gggtctgatt gagtttgtct tcttccgtat cgatgagata
    28561 acgtaaatca taaccgagca cctgactcgc tcgatcaatc gtttctttga caatcggata
    28621 ctgatcatag aaatcccgtc ccatccctag atactgggca ccttgaccag caaataaaaa
    28681 ggctgtttta gtcatttctt acaactcctg cccagcgaga ggcttcttct tgaattttct
    28741 tagcggctcc gtaatacaaa tcttttagga tttcttcagc tgtttcttct ttagaaacaa
    28801 gccctgcgat ttgacctgcc atgacagagc caccatccac atcaccgtga acaactgctt
    28861 tggctagggc acctgctccc atttgttcaa agatttctaa atcaggatct tcctgcttaa
    28921 aggcatcttt ttcagccagt tcaaaatctc tagtcaactg atttttaata gcacgaacag
    28981 catgaccaaa gtgctgagct gaaatcgtag tatcaatatc ccttgctttt aaaattttct
    29041 ccttgtagtt tggatgggca ttcgactctt ttgcaactac aaaccgtgtc cccacctgta
    29101 cagcctctgc acctagcata aagccagccg cagcaccttc accatccgca attcctcctg
    29161 cagcaataac aggaatagat atagctgtgg ctacctgtcg caccaaggtc atggttgtta
    29221 atttaccgat atgcccccca gcttccattc cttctgcaat aacagcgtct gcaccgattt
    29281 tttccatgcg tttagctaaa gcgacactag gaacaacagg aataacgatt atcccagctt
    29341 catggaaacg ttccatatac ttgcttggat ttcctgctcc tgttgtgaca actttaacac
    29401 cttcttcaat aacgagatcc acgatatctt ccacaaaggg agataagagc atgatgttga
    29461 ccccaaaggg tttatcagtc aatgatttga ttttatcaat attggccttg acaacttctt
    29521 tcggggcatt tcccccaccg ataattccta atcctccagc cttggaaaca gcccctgcca
    29581 aatcaccatc agcaacccag gccatccctc cttggaaaat aggatagtca atcttcaata
    29641 attctgtaat acgcgttttc atagtgcctc caaccttcct tgcttacgta atagttcgat
    29701 ttcaccataa tttgacagtc aaactattac ctaaacaaga gggagtgggt ttctccctac
    29761 tccttctact aatattctgc ttattttgct tgctcttcaa cgtaagcaac caagtcacca
    29821 actgttttca agtcattttc tgcttcgatt tggatatcaa aagcatcttc gatttctgag
    29881 attacttgga acaagtccaa tgaatctgcg tccaaatcat caaaagttga ttcaagtgtt
    29941 acttctgatg cgtcttttcc aagttcttca acgataattt cttgtacttt ttcaaatact
    30001 gccatgatag gactccttta aaataaatag tttttttata acaatgtgtt caccacatga
    30061 ttacctaaat tgtaagaatg agcgtgcccc aggtcaagcc tccaccgaag cctgatagaa
    30121 gaacagtctg gctaccatct aaagggatga gaccttgttc tacacactct gaaagtaaaa
    30181 tcgggatact ggctgcactg gtattgccat attccatcat attggctgga agtttggctc
    30241 ggtcaacacc aatttttcta gccatcttat ccaaaatacg gtcattggct tgatgaagta
    30301 gcagataatc caagtctgtc acctctatag gagattcatc aatagtctgc ttgatagact
    30361 tggctacatc tcgaatggca aaatcaaaga ctgtgcgtcc atccatcttc aaaaacgaat
    30421 ctgcactttc ttgatctgaa aatggagaat gcaaacccga atgcccataa gttaaacact
    30481 cgctgcgact tccatcgcta ttgagactct cagctaagaa atgctcttgc tcgctagctt
    30541 ctaacaagac accaccagca ccatctccaa acaacacagc tgttgatcga tccgaccaat
    30601 cgactgcctt agagagggtt tcactaccaa tcaccaagcc tttttgaaag cgaccagaag
    30661 cgataaactt ttcagcagtt gaaagagcaa atacaaatcc actgcaagcc gcggttaagt
    30721 caaaagcaaa ggctttatta gcgccaatat tagcttgaac acgagcagct gtagagggca
    30781 tcatcgaatc tggagtaatg gtagctagga tgataaaatc cagttctttt cctgttattc
    30841 cagcttttgc catcagtttc ttagcaacct ctgtagccaa atcactggta gattctgttc
    30901 ttgaaatatg cctttgtcgt attcccgttc gacttgaaat ccactcatca ttggtatcca
    30961 taatctgagc caagtcgtga tttgtaacca cttgctctgg cacataatga gcaacctgac
    31021 ttatttttgc aaaagccatt atttcaaatc ctccaaaaat tggtaaagat tagtcaagcc
    31081 tttacccatg acagcaattt cttcctcgct catgccatca ataatttttt ctaccatggc
    31141 cttgtggaag cgtttatgca gtctatgaat caagcgaccc ttctttgtca aatgcagatg
    31201 caccacacga cgatcctgtt ctgaccgaac tcgctcaatg tagcccttac gctctaaatt
    31261 gttcaaactt gtcgtaacag ttccaagagt taccatcaac tcttttgaca cttgacttgg
    31321 agtcacatct ggagccttac cgatgacatc aatcgtatgc atttctttga tggagatatc
    31381 cttaaaacga ctacctctca agttcacttc ctcaattaca aggacattgt taaatataga
    31441 tgttaaatat tcattgattc gttggtagtc catttttcat atccctcctt cttcaaaaaa
    31501 acttttacaa tcaaaacatt tgacagaaag aattatatca aaattcaaag aatgatgcaa
    31561 gtatttttta ttttcctgta aatttaggtc ttcttctctc cgaatgagct cgcactcctt
    31621 ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt cagttctaaa gtagcgtaac
    31681 cctgccagtc tttaaattgg ctctcccaaa ccaacttctt aatggcagca taggaattac
    31741 ttgaagcacg tcttaatttt ttaagaagct gttctctcgt cttttcaagt ttttcagctt
    31801 cagaaacgcg gtaaaccaac ccccattcta gggctttttc tgccgttaaa gcctctcctg
    31861 tcatagctaa ttgagcagca cgcgtcacac cgatgctgcg gctcaagaga tgaattcccc
    31921 ctgcatctgg agccaaacca acaccaacaa aggcttggat aaacttagcc ttatccgtcg
    31981 ccaaacagaa atctgccgca acagccatat tcgctgcggc acctgcaaca gcaccgtcaa
    32041 cttccattaa aacaggtttg gctatttgct tgattttata agaaatcgta ttgaccaact
    32101 ctgcgatttt tgtcaatgat ggaatatcat cctcatccac tgctcgcttc atctctacca
    32161 aatctccccc aactgagaag acctttccat tggcattgat taagataaaa tgcacagctg
    32221 gattttcttc tgccaaagtc agagcttcta aaatctcctc acacatggga atatgaaaac
    32281 catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca agctgataaa
    32341 taatgtgttc catcagggct cctttttatt ttataagcag tttatactct tcgaaaatct
    32401 cttcaaaccg cgtcagcttc catctgcaac ctcaaagcag tgctttgagc agtctgcggc
    32461 tagcttccta gtttgctctt tgattttcat tgagtgttaa attatttcac tgtcaaagta
    32521 tatctttttt aggagcagaa agcaagaaaa aatcgactga aagtgtctca gccgataaga
    32581 aaggctcgct ttaggctaga aagcatctta ttttcttcat tgaaaaaagc ctttctttct
    32641 gctataatcg tataaaatac ttactttagg agttcttatg aaagttgtta aatttggagg
    32701 tagttctctt gcctctgcta gtcaattaga aaaagtttta aacatcgtca aaagcgattc
    32761 agagcgtcgt tttgtagtcg tttctgcgcc tggtaaacgc aatgctgaag atactaaggt
    32821 tacggatgcc ctgattaaat actaccgcga ctatgttgcg ggtaacgata ttagcaagag
    32881 ccaaagctgg attatcgacc gctatgctgc tatggttagt gaattgggac taaaaccagc
    32941 tgtgctagaa aaaatttcta aaagcattca cgccttggcc actcttccta ttgaagaaaa
    33001 tgaatttctc tacgatactt tcctagcagc cggtgaaaat aacaatgcca aattgattgc
    33061 tgcctacttt aaccaaaatg gtatcgatgc acgctatatg caccctagag aagctgggat
    33121 tgtggtcaca agtgaacctg gtcacgctcg catcattcca tcaagttatg acaagattga
    33181 agaattgaca aacaccaatg aagtccttgt cattcctggt ttctttggtg tcactaagga
    33241 aaatcaaatc tgtactttct cacgtggagg ttctgatatt acaggttcta tcattgctgc
    33301 tggtgtcaaa gctgacctct atgaaaactt tacggacgtt gatggtatct ttgcagccca
    33361 ccctggtatt atccaccaac cacactcgat tcctgagttg acctaccgtg aaatgcgcga
    33421 gttggcctat gcaggcttct cagtccttca tgacgaggct cttcttcctg cctaccgtgg
    33481 aaaaattcct ctggttatca agaataccaa caaccctgac catccaggta ctcgtatcgt
    33541 tctaaaacac agtaatgatg aatttccagt tgtgggaatt gctggtgact caggctttgt
    33601 cagcattaac atgtcgaaat atctcatgaa ccgtgaggtt ggatttggcc gcaaggttct
    33661 gcaaatcctg gaagaactta acatcggttg ggaacatatg ccaacaggta tcgacgatct
    33721 ttctatcatt ctccgttctc gcgaactaac tcccatcaag gaagaagaaa tcctgcgtca
    33781 gttggttcaa aagactaaag tagatcatgc agaaatcgaa cacgaccttt ctatcattat
    33841 gattgttggt gaaaaaatga agagccacat cggagtgact gctactgcga cacgcgctct
    33901 atctgaaaac aaaatcaaca tccagatgat gtctcaaggt tctagtgaag tttctattat
    33961 gtttgttgtc aataaagacc aagagaaagc agcaattaaa gccctctaca atgccttttt
    34021 tggaaaaagt aaggaagact aagcatggcc caatcactta acaaaacagt actcctcagt
    34081 acgacaggca cttcctacct ctctatcgct gggaaagttg ggaaattcct tgtcggagat
    34141 caggctttgg aattttaccc agatgtcaat gtcgaacaat ttatccagat tccttggagc
    34201 catatcaatc agattggagc caatgtcact ggacgcaaaa tcagccgcca cttcgaagtc
    34261 tttacagaca aaggaaaatt cctctttgct tcaaaagact caggtgccat tctcaaaatt
    34321 gcacgcgaaa aactaggcaa tgacaaggtc gtcaaacttc cgaccctgat tcagacaatt
    34381 agccaaaaat ttaaaaatct atttgcaaaa aagtagaaac tttagtataa tcatagcaac
    34441 ggataagtag gttatcttct tctttcagaa agtctgcggg tactgcgagc agataggagg
    34501 gataggtgaa attctaccgt tagtgacaat tacatacaca atcaagtgca cacctgtgaa
    34561 gttggatgga accgtggccc tgccactcca acgttttgtc agggtgcttt tttcataaag
    34621 gagttcttat gttagatatc aaacgtattc gtacagattt tgaagctgtc gcagaaaaat
    34681 tagctacacg tggtgtagat gctgctgtct tgaatgaaat gaaagaaatc gatgctaaac
    34741 gtcgtaacat cttggtcaag gttgaaactc tcaaagcaga acgtaacaca gtttctgctg
    34801 agattgccca agctaagcgc aacaaggaaa atacagatga caagattgct gccatgcaaa
    34861 atctatctgc tgaggttaaa gccttggatg ctgaattggc agaaatcgat gctaaattga
    34921 cagaatttac aacgactctt ccaaatatcc cagctgacag cgttcctgtt ggggctgacg
    34981 aagacgacaa tgtggaagtt cgccgttggg gtactccacg cgagtttgac ttcgaaccta
    35041 aagctcactg ggatctcggt gaagaccttg gtatccttga ctgggaacgc ggtggtaagg
    35101 taacaggcgc tcgcttcctc ttctataaag gcctcggtgc tcgtttggaa cgtgctatct
    35161 acaactttat gttggatgaa catggaaaag agggctatac tgaagtcatc acaccttaca
    35221 tagtcaacca tgattctatg tttggtactg gtcagtatcc aaaatttaag gaagatactt
    35281 ttgaactcag cgataccaac tttgtcttga ttccaactgc tgaagttcct ctgacaaact
    35341 actaccgtga tgaaatctta gacggcaaag atcttccaat ctacttcact gctatgagtc
    35401 cgtcattccg ttctgaggct ggttctgccg gtcgtgatac gcgtggctta atccgcttgc
    35461 accaattcca caaggttgaa atggtcaaat ttgccaaacc tgaagaatct tacgaagaat
    35521 tggaaaaaat gacagccaac gctgaaaaca ttcttcaaaa actcaacctt ccataccgtg
    35581 tcgttgctct ctctactgga gatatgggct tctcagctac gaagacttac gacttggaag
    35641 tgtggattcc agcacaaaac aattaccgtg aaatctcaag ctgttcaaac acagaagatt
    35701 tccaagcccg tcgtgcccaa atccgttacc gtgatgaagc agatggcaag gtgaaactcc
    35761 ttcatacctt gaacggttct ggacttgcag ttggacgtac agtggctgca attcttgaaa
    35821 attaccaaaa tgaagatggt tctgtgacca tcccagaagc acttcgtcca tacataggtg
    35881 gagctgaagt catcaaacca taaaaaaata aggtttagct atttctagct agaccttttt
    35941 tcgtaaccaa atcagataag cacctagtac aaagaataaa atagttaggc atataatggt
    36001 ttcagtcaat accaggtaat ccagaaatgg aagtttcaaa attccctgag ccatcttgag
    36061 cgaggtcgct gtgataatgg ttgggaaggt gagggctgag aaggctggtt gaaaaccttg
    36121 ttttaaaatg ttgggcagac gagttaaaac aaagaaaaag aaggattgag aagccaaaat
    36181 catgacaatc aagacccaag tcggcaggct ggttcctcct actcgaacta gagaagccaa
    36241 gagtagagag aaaggagcac agtagattcc ttcttgtcca agcaaggcta gtgggagtgg
    36301 atgtttcttt aaatcgctat aaataagggg atagagatag aaggtcaaga gaaaaccaaa
    36361 actcaaggtc gcataggcaa tttcgataat acctaccaga ggataggtca aggcagccac
    36421 tgctatcccc acatagagaa ccgtccaact tggagtggca tgaaccctcc gccctggaca
    36481 agcaaacttg atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg aaaaccacca
    36541 aatcccttgg gctaccaaag gaagataaga gaatacgcga aagacatagg tcgataaaat
    36601 catcccagcc ataggaaagg ttgccattcc tgacaaaaga gggggcttgg tcaattcttg
    36661 cttggtttct ttccaattaa agagatgcag aattagaaag taaatccata aaaccaaacc
    36721 agtcagacta aaaagatggg atagaaccgg caacgtatct aaaataagat ttccagctcc
    36781 tgccaaacct agcaaacaac ctgaaaatac taaggggagt tttttcatcc taacctccaa
    36841 taatcatgtt agtttcagta taacataaaa gcgcttaaat gaggatttaa aaaacgaagt
    36901 ccgcttattt cagacttcat tttactcaga tatgaattag gcataaggtt gcaattctgg
    36961 attaattggt gtattagcta agttgttggc atagttacag aggattgcta ggctgacacc
    37021 aaaaaccaca tccaaggcat tttgttgagt gtagccagct tctaaaaact cagacaaggc
    37081 ttcatctcct acacgaccct tggtattgat aactgccaag gtaaacttag ctagggtatc
    37141 caatttagga tctgtttcaa ttggagtacg attgcgaaga gcttgaatca agtcatcatt
    37201 catctggatt tgtttgatgg aaaaggctgt gtgacctgcg acacagaagg cacaaccatt
    37261 ggtcacggct gccgtgattt gcaccacttc acgctcaacg ggtgtcaggc tgttgcgacg
    37321 gtggatagat gagacaattt ggtaggcttc taaaacagtc ggggcattgg ccaagagacc
    37381 gattaggttg ggaatatagc cattgttgtc tttttctact gtctcaagaa tttctttcac
    37441 ttctgctggt gctgactcta ctgtatggat agtaaatgtt gtcataagat acctcttttc
    37501 ttattattga cactaatatt attggaaaat cttataaaat cctgattcct aagtttatct
    37561 aagataaagc tttattctct cataagattt tcgttgttat attagtttat cacacttcca
    37621 atcacttgca taatatatat tatatatcag gctgataaaa attatttata ggcaaaaaaa
    37681 tcacacgagc tgtgtgattc cattatttgt caaaatactt tttagtttca gcaataacga
    37741 ctggcgacaa gaccaagagg gcaatcaagt ttggcagagc catcaaggcg ttaacgatat
    37801 ctgcgataat ccagaccata tccaactcga taaatcctcc taacaagacc atgagcacaa
    37861 aaaccacacg gtagagccag ataaagcgaa ccccaaagag gaactcaaaa cagcgttctc
    37921 cgtaatagtt ccaacctaga atcgttgtaa aggcaaaaag tacaaggaag atggtcaaga
    37981 gggcaggccc aaagtgtgaa aagactgttg agaaagctga ctgagtcaag gcaaccccat
    38041 tcaagtcacc actccaaact ccagttacca agatggtcaa accagttaga gtacaaatga
    38101 tgagggtatc aataaaggtt cctgtcatgg aaatcaaacc ttgctctact ggttcatttg
    38161 tcttggcagc tgcagctgca ataggagcag aacccagacc cgattcgttt gagaacacac
    38221 cacgcgccac accattttga atagccatcc gaacgctagc accagcaaat ccacctaccg
    38281 cagcaagggg actaaaagct gaggtaaaga ctaaagcgat tgtgccaggg atttttccga
    38341 tattaaagaa aataactgta agagttccta agatataaat gagggccata aaaggaacaa
    38401 cagtagttga aaccttagaa atagacttga gtccaccaaa gactgcaatc gctacaaaga
    38461 cagacaagac gagagctgtg atggctggcg aaatcgtcgt tgtattttgg atagattctg
    38521 caatcgagtt gacttgggtg aaggttccga ttcccaagag aacaaccaat actcctgcta
    38581 ctgcaaacaa aacagcaagt ggtcgccact tttctcccat ccctagaagg atatagtgca
    38641 tgggacctcc cgctactgca ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc
    38701 cttccgcata cttggtagcc attccaaaga aagccgccat ccacatccaa aatagagctc
    38761 ctggtccacc aaccttgata gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg
    38821 atgccaaggc tgtacacaga gctgcaaaac tggatacatc accatgtccc ttatcctgga
    38881 taaaaataag ctgaaaggcc ttgggcagac gcaaaacctg caagagtcct agccgcatgg
    38941 ttaggtaaat ccctgttccg accaataaaa tcaagagggg cggtccccaa gcaaaagcat
    39001 cgattgattt aagcaattct aacatttcct tctcctatcg tttcaacccc aaaagaaaga
    39061 gcacatgcaa gatacatgta ctctggaatg cttagataaa tgctaaaaag cggtctatcc
    39121 tagctctgtc cttttacctg agagtttgag cagttgcctg ccttgcccct tcggtgcctt
    39181 tacggtctct ccagagttcc gtccatttac agtcatggaa aatcaaacga ttccccactt
    39241 ctattaaact tcattcggtg ttggtattta attgattctt attttacaaa aaatgttggc
    39301 ttttgtcaat gtgttttgga aaaatcttat ttgactctgt agaagaagga ttgagcattc
    39361 ccatgtgcgt ggggtgctat tagttattaa atcaagctgt ttcactctta ttccacacga
    39421 tataagaatt gtcttggatc accatgtagc ggaaatgcag catggaagat gattcgatct
    39481 ttactaatat ccgaagcatc cgtgaccaac tcacccctag atgtcccatc cggtattcta
    39541 acacctgctg gtacgaactg aataaagatt ccaaagtctg gtgctgaata acgagctcgt
    39601 aaaaaaccat ttacaaattc tgattctgta aggtcaactt taaatttctc tggtgctatc
    39661 aagccatctt tactaaagac taagtcttct cctccatgac cattactcca tcttccctca
    39721 atactagaaa aatctccatt tttgatggcg tttatatcca ttttccctga agctgttttg
    39781 ctagttgaag atgctgttgc agcaaatttt tctgttacag atttccaggt cagactagat
    39841 agattcattc gagtacctgg actgacttca tcctctgtct tagcttcttt aaccttctct
    39901 agtttgtccc cactactatt caatttccaa atggtatgaa tggtctgtgg atctcctgcg
    39961 aacggattcc actgggtata gagattacca ttttctaata gataggcctt gaaacgagtt
    40021 ccaagcatct gcattttatt ttcagcattg gtgaggcgaa taactttctg atctttgagt
    40081 gtaaaaatat ctaaaatctc actaccattt ttcgaagaga caatcaattc atcaatgcca
    40141 tttttgtcca tatcaaccat tgcataggta aaggcttgac tgcgttctgc cattcctgca
    40201 tagatattag cagttggatt gatgttatcc ttaacctctg acttatagtc taaaataaat
    40261 tcataatcct tcaaaatgct attataagga ctgtaatcta cacttggttt ccccttatct
    40321 gcactgcttc ctcctggaag tttatctgaa actgacttcc agcttaaggt ctctagagaa
    40381 atgggcgaac cgaccccaag atctgctagt tcttgagctt ccttatcctt taccaaagac
    40441 tgcccatctt cactaaattg gtagtgagca tagatgtgac tcgttgctgt accagctcca
    40501 cgatagagca gagaattgtc tggaaggata ccgaccgtca ttctctcacc aatctggtct
    40561 aaacgatttt cttgattggt caaacgaatc accttttcgt ctttcagcgt acgaatatca
    40621 agtaaactct tttcgccact cttcatttca agagcaagta aaagttcatc gactccatca
    40681 ttattcaaat ccgtcttgtg gtaaaccaca tctgtataaa agccttgaga agacttcact
    40741 tgactagcca gaggattgac atccatacct gtcggcaagg tgcctttcgc tactttttca
    40801 tagtcatcca aaactttatt ataggaactg tagtctatct ctggagtcgc tacagatgtt
    40861 tctgatgttg aatcagatgg tgtttctgcg cttttgttcc aaggtaaata ctgacagcct
    40921 cctagaacta gtgtcagtaa aagtgttgtg gctaataaaa actttttcat attactcctt
    40981 tatctaagag aaaggaacca gcctgtgcca attcctttcg ctatttttat tgtatcaaaa
    41041 tgttgcctat aaacaaatta ttttccgttt ctatttattt gttataagcc atttctcaca
    41101 agctatcatt ctagcgaacc cacttacctg agccgtctac tttgtagcca tctggggtac
    41161 gttcgttgac tgccatctta ccatctgatt tgaggtagta gtcacctacc catgtattac
    41221 gggcgtaact tccttctgat gttaggtaat agtaagattg gtagctacta tcatagatcc
    41281 actcactctt agccatttta ccatctgatt tgaggtagta gcttccagac caagcattac
    41341 gtgcatagct accctctgct gtcaagtaat agtatgattg atagttattg tcataaatcc
    41401 attcgctctt agccatgtag ccacctgact tgaggtagta acttccagac caagcatttt
    41461 gaacaaagtt acctgaagca ttgaggtaga agtatgaatt gtactgaaca tcaaaaatcc
    41521 attgattgac tactttcaca ccagctttgt agtaagaaga acctgaccag ccagtctgac
    41581 ctgagctaga gttagtagct ggttttgaag tttcagtagc tgttggtttt tccgtttctt
    41641 taggagttga agagtctaga ggattgacat atttttgatc tttaacagtg aatgtcgctg
    41701 atttgccaat aaatttatac aaaacacttc tgtaatcgtc ttctccatga aattgtaagt
    41761 aatattcacc gtctggaaca tttgcaaaat tagtccaacc ttgctttgta atcggaacag
    41821 atgatctatc gaacctttca ttgaaagtat cttttctagg aatttgccct gtttttgcat
    41881 ttacaatatc tgcatatata tatacatgat agtgtacagg tacattttca acagtcatat
    41941 ccacatagat acttccattt tctgatcctg gtgtaatctt agattttacc tcatgattct
    42001 cggtacgagt aatttccgca tgcactgttt gtgctgaaaa agggagaact cctgctgtgt
    42061 cgattgttga tactactccg actgttgaaa gtgccacagc acatactaat aatttctttt
    42121 tgttcatgtt attcatttcc tttattttat ttttaatatt tatttgttta aaacctatca
    42181 ctcatctatc cgacaaaacg atgattcttt acacgaaaga caactgattg gccatcgtat
    42241 accttgccat cttgtccttt tcctttgata acaaagcgat aactaccatc tggagtggca
    42301 ctaaagaagt gacgagtttg aaagacacgt aagcttggat tgtaggtcac agtaaactgg
    42361 gtttgtgtag aaactgaacc tgtatttgca tcaactaagg caaaatcata ggctacttga
    42421 taagatgagg atggagtgct aggtagagcg atatcaatat aggtcatagc atcattgaca
    42481 ttgtaaaggc ttattcctaa tgatgttgct tgtgaactgc ttgctgtatg agttccctca
    42541 gaactaatcc acttgcctga tccatccact tggtagccat ctggtgtacg ttcgctaact
    42601 gccatctttc catttgactt gagataataa tcacctaccc atgtattacg agcataactt
    42661 ccttccgatg ttaagtaata gtaagagctg tagttattgt cataaatcca ctcaccttta
    42721 gccatctttc catttgactt gagataataa tcacctaccc aagtattacg ggcgtaactt
    42781 ccttccgacg ttaggtagta atatgaactg tagttcttat catagatcca ctcgctttta
    42841 gccatcttac cgtttgactt gagataatag ttgcccaccc aagtattgcg agcatagtag
    42901 ccatctggag ttaagtaata ataagctcca tagttcttat catagatcca ctccttctta
    42961 gccatgtagc cacctgactt aagatagtaa tcaccctccc atgcattttg aacataatta
    43021 cctgaagcat ctaggtagaa gtaggaatta tagttcttat cgaaaatcca cttactagtg
    43081 actttagtac cattttggta gtaagatgat cctgaccaac cagacggaac tgtagattgt
    43141 ggatttggag ttggatctgg ggttggtttt ttatccgatt gtgggattgg agctggattt
    43201 ggagttagtt ttttatccgt cagattctta atcgcctctt taaactcttc tacagttccc
    43261 ttccactcta tgtaattggc accagactta ttctcattag caatatagtt gcgagcagat
    43321 tcttggaaat aagataattc tggagtggtt tttgtggccc aaataatggg acgattagca
    43381 gtacctctcc aatttttaaa tacttctgaa tccttcggaa caagaatata atattttgca
    43441 gctgtagtca gcgtttgtgt gtcagcttgt accgtttttg ctgaaaaagg tagagctcct
    43501 gctgtatcta caagagacac aagaccaaat gttacagcgg ctaggctggt tactaaaact
    43561 gtttttgact tcattatcaa aacctccttt aaatttttat tatttaggct tacagtaagg
    43621 gatggactct actatattta tgaaaagagt atctgtgttt atactttatt tgattagcaa
    43681 atgaaatttt tatgaaaaaa gtatacttaa tctttttcct agttattaga ctaaagactt
    43741 tctattttat catttccacc tacttgtcaa atatgccttt tctcctattg tccaattttg
    43801 atcataagga tccactgacc attttgttgc tagcaaaaaa aacggaaact tactataacc
    43861 aatttgagct tcgactagat cggtggtcgt attgtacctt ctatctcgtt tccacttacc
    43921 aattttttga cttctattcc tattccagat aaaaagttcc tctggtagtg cataaattct
    43981 accgtcatga gaactccctc tcattacagg ataaaggtac tgatgcaact gatattcaca
    44041 tcgcttattt aagagccaat atcctagaat catatagaag ggatttatct tttcaagagg
    44101 taagataatt ggcaaaggta aagcattact cacatccact ggttttgtaa gatacattat
    44161 ccataacaat actgatgaaa aataaaccat aaatgtatgt agtggtattc tattcaaaac
    44221 aatcatatag aatttgtaat tctttatctt tatcaaacag cgtcttacat taatactgcc
    44281 cacaattatt ttatatagaa aaatcacaaa gaagattggt gaaatatata caaaaactaa
    44341 aaaagatgtt aaaacgataa actcagctat tccaataaca gctggatatt gaactttata
    44401 tgtttcaaaa tcctttaata caatgaacca tggagcgata aaatacaagc acgagaatat
    44461 tagtaaagca atttttaaat agaaaatatt caagaattga cttaatttta aaatgtttgt
    44521 tctcatataa cttcctgctc tcctatccat ccaatttatc atttcttaaa accatgataa
    44581 tcttcaagca gaattccccc tatatttgtt taataatttg tagataaata ttaatctaaa
    44641 cttattaaaa taatttagaa tacttacttc tacttcctta gaaatatttt tctggattgt
    44701 ataagttcga gtaaattcaa ttcggctcat attattctta aaatataact catataaaaa
    44761 atctacaaca tcaattcttt actattttta cataatttaa cgtttcaaaa gtaaattaaa
    44821 tgtctgccga atcatctgcc caccaattgt atccctaaac ttatcttccc tagataactc
    44881 atctaacatc tcttctagac taacagcctt aatatgacct ggccaaaaga cattatccaa
    44941 tctacctgca cccttatagg taggcattaa gacaacacgt gtttccaagc gataatattt
    45001 taataaactc gcaaatttat gggaaaagct ttcttctgca taagacatgt taggggacat
    45061 ttttttaggt ctgcctattt gagcacctgt agcactatct atagtgtaca ataaaccatc
    45121 cgcttctcta taaatcacat ttcctgaagc ataaaatttt aaatctacta accaaattgt
    45181 agatccagaa acaataacac aatcaatatc cgcatctacc ctttcatctt ctaaattgag
    45241 attatgaaca gaccaaaagg taactagttt ttctaaaagc cctttttttt gtaatgcttt
    45301 agcaaaattg atttcaccct cttgaccaag attaacagca gattcatcga atcctgattt
    45361 attcaatcct tttccaggtt ctccaaacat caattcatgc tggtaaagtt gataagtctt
    45421 aagtttttgt ggttcaaatc ccgtcatttt accattatga gttgccttgg aaatactggg
    45481 aagttcacta tgtttaggtg tggaaggagt aggtggttct ggagaaacct tagcattttg
    45541 tcttctgccc caaagaaatc ctaatataaa atatagtatg tttctgcgat gcttaaaaaa
    45601 gcgcaacaat ttctccacct cctaaaaaat atcttattta gcaatagtca ctttacttgg
    45661 tctaagaacc ctgttattta acaaatatcc tttttgcaat acttcaacaa tattattttc
    45721 actttgttca tcagtaactg ctactgtatt aacaacctca tgcatacttg ggtcaatcat
    45781 tccactatta tcaataactt gtaatccatt caatgaaaga atttgcagaa gctcttcagc
    45841 aattgaagta agcaaatctg atttttcttc agatgattca atacgatcaa taacttgaag
    45901 aatctgtttc ataaatggag caaatttctc acccttatct aaatcctgtc tatagattaa
    45961 atactgcttc aattcttcaa tcagactatt tttggcttta tcttccaata aacggcgtgt
    46021 aattaaagat tcgatatctt caactttatc tacgatatac gaaacttttt catgcaatac
    46081 ttcttcactc ataaaaactt tatcctttct tctatttatt tgccatcctt tagggtaatt
    46141 gtaatacaag acaatatttg ataacaatgc caaacttaat gcgttactaa tccaaaagct
    46201 atgtacaaac ttatcaaaga aaccactcaa aaaccaattg gctgcaaaaa ctaagatgaa
    46261 catgagacat cccatacctt gtgaatggcg tgttgtggca taattgatat aatttttatt
    46321 ttcaataaat tcattgtaaa aatcattatc tataatttta ttttcaatcc ctaaactttg
    46381 tgccttggcc aagtaagctt ctgcaatgcg aacacgcgcc ttattatcta gaactgcttc
    46441 attctcatat tttgttcgat caatatattt tctaaagaaa gcctcaacgt ctgtatcagg
    46501 tgcttttttc atacgctcta tcgtgatatc taatagtaac ttaaaataca cttgctggaa
    46561 atctttattg ttaccatagc gttcgtttaa ggtatctaaa taattctcac actcatcttc
    46621 agatttcaaa aatagaatac ctgcataatg tagtttatgc tctagtgaat ctgaaacatc
    46681 tactaatttt cttgcaaaat tataggcttc ttcattatct ccaatttctc tataagagca
    46741 tgataaaagg taataaatat attcttcatt atttttattt tcttctaaat aatgatcata
    46801 cagatatcgc acatctttta ctgcctgttt atactgatca aataaaaata gtgatcttcc
    46861 ctttttcaaa agataaaagg aattattaga tagttcaaaa gcttcctgat aatactgaat
    46921 tgcaaacttt tgctgattct gttcacaata aacatcacct agcaaagata agatatccgc
    46981 atttttaggt ctcaatctat tagcttcact aatagctaat tctgcatctg ataactttct
    47041 atcttcaagg ctaatcatag ccttgaaata ccaaacatct gcatcatttc gatttatttt
    47101 taaggcttct tcaatgtagg aataagctaa tgatttcttt cctgaataaa aggcctgtct
    47161 agctgaatca agatagcttg aatttgaatg attagttgga gtcgaatcag ggactccttc
    47221 agaactttgt ttagaatttt ctaattccct atcatatttt tgtctggttt cttcactttc
    47281 aaatactttt tctgcctgtg caatgacttc catctttctc tcagccattg atctttgatc
    47341 aatatttggt gaacctgtca actggcgata gcgtcttcta tttgacttga tacttttctt
    47401 aacatcatct aatgaacttg tttctgaaat ccctaactct tcataatagt ttatgaccga
    47461 cattgtgttc ctcccctaat caatagttaa atcttttatt ttacgactag ctacttctac
    47521 agcactttta tcgagatttg cttgtctctc tacttcaaaa gtccctaata agtgattatc
    47581 cgaatcatgt acctctacaa aaactgtttg atcgacatca taatgataag ttaccttcaa
    47641 aaccgttcct gcaggccagt cacctggtaa tttaatggta ctctctccta taaccgtaac
    47701 aaattgcgga tcctcgtcat ccccttgagt aacctctact aacacatttt gttgattatc
    47761 tacaactgtt gaaaaatact cagagtactt gtttggtacc ttggaatttt taggaataat
    47821 aattgtattt cttttcacac cattcgaaag agccagagtt cccaaggctt gagaagtgac
    47881 atccgaaaca actaactttt ctgctaatcc acgaactgtt tcactaacat ctgcactttc
    47941 tgatttatta gcgatttcca atgctgcttg aatagctgct ccttgtgcta cagcttcatc
    48001 tggattaata gaatatacaa tcttcttccc aattttctct tccaattttc tttgtaccat
    48061 tggcattcga gttgaaccac ctattaatag tacttggtca atttcatccc atgacattcc
    48121 agactcttca acaacatcat ctaacagctc ctctgtacga ttcattagac tttttgttgc
    48181 ttcttcaaat tctacacgtg ttatcggaat tttataactt tttcctttat agtcgagaaa
    48241 gacatttgtt ttttctacag ttgttagtcc tctctttgta ttctctgatt tttcacgaag
    48301 taaggcagta aaatgttcat ccgtataaat atcttctgct ccctgttctt ccatcttttc
    48361 agcaataatc attgataagg cgttatcaaa atcaaaccct cctagattcc gatctccatc
    48421 ggtggcaata acatcaaact cgccatcttt tattttcatt aatgtgacgt caaaagttcc
    48481 accacctaaa tcatatacca atactgtctc atttttttct gctgatatac cgtatgcaag
    48541 tgcagcggct gtcggttcat ttaaaactcg taaaacatta agtccagcaa tttctccagc
    48601 atgtttcgtt gctgttctac gggcatcatc aaaataagct ggaacagtta ttacaacatc
    48661 ctctactttg tcaccaaggg cattttctgc tccttctttt aatcttttta aaatgattgc
    48721 tgaaatttcc tctgctgtat aaacagtatc actaggagaa tcaaacttcc aatttggatt
    48781 tcccatttgt cttttgacat actgtacaac tgcttcaggt ctacttgctg ccaaattttt
    48841 agcttgaata ccaacaagtg gttcatcttt cccatcaaaa tcctgaaaga atacaacaga
    48901 tggagttaaa ttttcaccat cttgattcaa taaaatttgt ggttgtccag tctctgataa
    48961 tacagatact gctgaataag tcgttcctaa atcaattcca attgctttgc tcatatttca
    49021 ctcctaatca ttatttgatc taaactgtgt tagtacctct gttaaaaact cacagtattg
    49081 taattctgca tcaatcttgc tctgattttt ccccaaagtt ctaagaccgc taactgttaa
    49141 gttactaagt tgagctccta atttctgacc caatgacaag tccgattctc taattgcttc
    49201 tctgaatctt ttattatctt ctgatatata gtattttttt aattgtttac tctcacctaa
    49261 agtatctata gtcagaagtt gtactgatcc ttgatttttt aaacgaaaac aaaactttac
    49321 atagtctttc tgatgatttg ggtgcgaaat aactagacat tcttcaatct ctttattgaa
    49381 caaaccgccg agttttattt gttcttcgtc aatttgacaa ggaagatcaa actcttttgc
    49441 tttctcaaca attttcgtct tgcaatattc taaatccaat cttggattgt tctcatcgga
    49501 ataatatctc cactcatcta tataactttt caattctttg tatttaatca tcaacaatct
    49561 ccttttatat cttattgttt acggttacag tatagca
//
LOCUS       DAVMMR010000007        46146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMMR010000007 DAVMMR010000000
VERSION     DAVMMR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.007"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(93..551)
                     /locus_tag="VYS74_000378"
     CDS             complement(93..551)
                     /locus_tag="VYS74_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV3788462.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(548..988)
                     /locus_tag="VYS74_000379"
     CDS             complement(548..988)
                     /locus_tag="VYS74_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788463.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1344..3293
                     /gene="mutL"
                     /locus_tag="VYS74_000380"
     CDS             1344..3293
                     /gene="mutL"
                     /locus_tag="VYS74_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV3788464.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3618..4085)
                     /locus_tag="VYS74_000381"
     CDS             complement(3618..4085)
                     /locus_tag="VYS74_000381"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV3788465.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4086..5291)
                     /locus_tag="VYS74_000382"
     CDS             complement(4086..5291)
                     /locus_tag="VYS74_000382"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV3788466.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5311..5946)
                     /gene="ribE"
                     /locus_tag="VYS74_000383"
     CDS             complement(5311..5946)
                     /gene="ribE"
                     /locus_tag="VYS74_000383"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV3788467.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5931..7031)
                     /gene="ribD"
                     /locus_tag="VYS74_000384"
     CDS             complement(5931..7031)
                     /gene="ribD"
                     /locus_tag="VYS74_000384"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV3788468.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7166..7280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7437..8030
                     /gene="ruvA"
                     /locus_tag="VYS74_000385"
     CDS             7437..8030
                     /gene="ruvA"
                     /locus_tag="VYS74_000385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV3788469.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8040..8603
                     /locus_tag="VYS74_000386"
     CDS             8040..8603
                     /locus_tag="VYS74_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV3788470.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8600..9277
                     /locus_tag="VYS74_000387"
     CDS             8600..9277
                     /locus_tag="VYS74_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3788471.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9432..10463)
                     /locus_tag="VYS74_000388"
     CDS             complement(9432..10463)
                     /locus_tag="VYS74_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV3788472.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10718..11401)
                     /locus_tag="VYS74_000389"
     CDS             complement(10718..11401)
                     /locus_tag="VYS74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV3788473.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11413..12357)
                     /locus_tag="VYS74_000390"
     CDS             complement(11413..12357)
                     /locus_tag="VYS74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3788474.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12489..15320
                     /gene="uvrA"
                     /locus_tag="VYS74_000391"
     CDS             12489..15320
                     /gene="uvrA"
                     /locus_tag="VYS74_000391"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV3788475.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15313..16374
                     /locus_tag="VYS74_000392"
     CDS             15313..16374
                     /locus_tag="VYS74_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3788476.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16506..16904
                     /gene="spx"
                     /locus_tag="VYS74_000393"
     CDS             16506..16904
                     /gene="spx"
                     /locus_tag="VYS74_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV3788477.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16970..17539
                     /locus_tag="VYS74_000394"
     CDS             16970..17539
                     /locus_tag="VYS74_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3788478.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17626..17892
                     /locus_tag="VYS74_000395"
     CDS             17626..17892
                     /locus_tag="VYS74_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV3788479.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17896..18315
                     /gene="ruvX"
                     /locus_tag="VYS74_000396"
     CDS             17896..18315
                     /gene="ruvX"
                     /locus_tag="VYS74_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV3788480.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18331..18627
                     /locus_tag="VYS74_000397"
     CDS             18331..18627
                     /locus_tag="VYS74_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV3788481.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDEWNMIEEVFNSFMEE"
     gene            18885..20135
                     /locus_tag="VYS74_000398"
     CDS             18885..20135
                     /locus_tag="VYS74_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3788482.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20132..20674
                     /locus_tag="VYS74_000399"
     CDS             20132..20674
                     /locus_tag="VYS74_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV3788483.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            20820..22352
                     /gene="cls"
                     /locus_tag="VYS74_000400"
     CDS             20820..22352
                     /gene="cls"
                     /locus_tag="VYS74_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV3788484.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22430..22573
                     /locus_tag="VYS74_000401"
     CDS             22430..22573
                     /locus_tag="VYS74_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788485.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22610..24148
                     /locus_tag="VYS74_000402"
     CDS             22610..24148
                     /locus_tag="VYS74_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV3788486.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24268..26475
                     /gene="nrdD"
                     /locus_tag="VYS74_000403"
     CDS             24268..26475
                     /gene="nrdD"
                     /locus_tag="VYS74_000403"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV3788487.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26487..26627
                     /locus_tag="VYS74_000404"
     CDS             26487..26627
                     /locus_tag="VYS74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788488.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26671..27177
                     /locus_tag="VYS74_000405"
     CDS             26671..27177
                     /locus_tag="VYS74_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788489.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            27170..27760
                     /gene="nrdG"
                     /locus_tag="VYS74_000406"
     CDS             27170..27760
                     /gene="nrdG"
                     /locus_tag="VYS74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV3788490.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27757..28374
                     /locus_tag="VYS74_000407"
     CDS             27757..28374
                     /locus_tag="VYS74_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV3788491.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28640..28948
                     /gene="rpsJ"
                     /locus_tag="VYS74_000408"
     CDS             28640..28948
                     /gene="rpsJ"
                     /locus_tag="VYS74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV3788492.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29165..29791
                     /gene="rplC"
                     /locus_tag="VYS74_000409"
     CDS             29165..29791
                     /gene="rplC"
                     /locus_tag="VYS74_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV3788493.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29816..30439
                     /gene="rplD"
                     /locus_tag="VYS74_000410"
     CDS             29816..30439
                     /gene="rplD"
                     /locus_tag="VYS74_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV3788494.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30439..30735
                     /locus_tag="VYS74_000411"
     CDS             30439..30735
                     /locus_tag="VYS74_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV3788495.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30753..31586
                     /gene="rplB"
                     /locus_tag="VYS74_000412"
     CDS             30753..31586
                     /gene="rplB"
                     /locus_tag="VYS74_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV3788496.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31690..31971
                     /gene="rpsS"
                     /locus_tag="VYS74_000413"
     CDS             31690..31971
                     /gene="rpsS"
                     /locus_tag="VYS74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV3788497.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            31983..32327
                     /gene="rplV"
                     /locus_tag="VYS74_000414"
     CDS             31983..32327
                     /gene="rplV"
                     /locus_tag="VYS74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV3788498.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32340..32993
                     /gene="rpsC"
                     /locus_tag="VYS74_000415"
     CDS             32340..32993
                     /gene="rpsC"
                     /locus_tag="VYS74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV3788499.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            32997..33410
                     /gene="rplP"
                     /locus_tag="VYS74_000416"
     CDS             32997..33410
                     /gene="rplP"
                     /locus_tag="VYS74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV3788500.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33420..33626
                     /locus_tag="VYS74_000417"
     CDS             33420..33626
                     /locus_tag="VYS74_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV3788501.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33651..33911
                     /gene="rpsQ"
                     /locus_tag="VYS74_000418"
     CDS             33651..33911
                     /gene="rpsQ"
                     /locus_tag="VYS74_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV3788502.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33937..34305
                     /gene="rplN"
                     /locus_tag="VYS74_000419"
     CDS             33937..34305
                     /gene="rplN"
                     /locus_tag="VYS74_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV3788503.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34383..34688
                     /gene="rplX"
                     /locus_tag="VYS74_000420"
     CDS             34383..34688
                     /gene="rplX"
                     /locus_tag="VYS74_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV3788504.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34712..35254
                     /gene="rplE"
                     /locus_tag="VYS74_000421"
     CDS             34712..35254
                     /gene="rplE"
                     /locus_tag="VYS74_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV3788505.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35272..35541
                     /gene="rpsN"
                     /locus_tag="VYS74_000422"
     CDS             35272..35541
                     /gene="rpsN"
                     /locus_tag="VYS74_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV3788506.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35826..36224
                     /gene="rpsH"
                     /locus_tag="VYS74_000423"
     CDS             35826..36224
                     /gene="rpsH"
                     /locus_tag="VYS74_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV3788507.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36416..36952
                     /gene="rplF"
                     /locus_tag="VYS74_000424"
     CDS             36416..36952
                     /gene="rplF"
                     /locus_tag="VYS74_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV3788508.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37036..37392
                     /gene="rplR"
                     /locus_tag="VYS74_000425"
     CDS             37036..37392
                     /gene="rplR"
                     /locus_tag="VYS74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV3788509.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37410..37904
                     /gene="rpsE"
                     /locus_tag="VYS74_000426"
     CDS             37410..37904
                     /gene="rpsE"
                     /locus_tag="VYS74_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV3788510.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37876..38100
                     /gene="rpmD"
                     /locus_tag="VYS74_000427"
     CDS             37876..38100
                     /gene="rpmD"
                     /locus_tag="VYS74_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV3788511.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38245..38685
                     /gene="rplO"
                     /locus_tag="VYS74_000428"
     CDS             38245..38685
                     /gene="rplO"
                     /locus_tag="VYS74_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV3788512.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38698..40008
                     /gene="secY"
                     /locus_tag="VYS74_000429"
     CDS             38698..40008
                     /gene="secY"
                     /locus_tag="VYS74_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV3788513.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40159..40797
                     /locus_tag="VYS74_000430"
     CDS             40159..40797
                     /locus_tag="VYS74_000430"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV3788514.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40914..41132
                     /gene="infA"
                     /locus_tag="VYS74_000431"
     CDS             40914..41132
                     /gene="infA"
                     /locus_tag="VYS74_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV3788515.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41157..41273
                     /gene="rpmJ"
                     /locus_tag="VYS74_000432"
     CDS             41157..41273
                     /gene="rpmJ"
                     /locus_tag="VYS74_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV3788516.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41291..41656
                     /gene="rpsM"
                     /locus_tag="VYS74_000433"
     CDS             41291..41656
                     /gene="rpsM"
                     /locus_tag="VYS74_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV3788517.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41674..42057
                     /gene="rpsK"
                     /locus_tag="VYS74_000434"
     CDS             41674..42057
                     /gene="rpsK"
                     /locus_tag="VYS74_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV3788518.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42100..43035
                     /locus_tag="VYS74_000435"
     CDS             42100..43035
                     /locus_tag="VYS74_000435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV3788519.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43047..43433
                     /gene="rplQ"
                     /locus_tag="VYS74_000436"
     CDS             43047..43433
                     /gene="rplQ"
                     /locus_tag="VYS74_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV3788520.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43698..43964
                     /locus_tag="VYS74_000437"
     CDS             43698..43964
                     /locus_tag="VYS74_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV3788521.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43974..45311
                     /locus_tag="VYS74_000438"
     CDS             43974..45311
                     /locus_tag="VYS74_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV3788522.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45555..>46146
                     /locus_tag="VYS74_000439"
     CDS             45555..>46146
                     /locus_tag="VYS74_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3788523.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSV"
BASE COUNT        13874 a         8749 c        10156 g        13367 t
ORIGIN      
        1 tcctgttctt atttcatttc actatagtaa ggtgcagcag gataaagaaa aaccgagaag
       61 tttctttctc ggatttgctt attatgaggt tcctattttc tatgctccaa gagttgattg
      121 atgtgatcta cttttttagc ctctctttta tagagaatag cccagatgat gagatagaca
      181 atcgcaaact cgataatgag ctggaggtag aaaatccagt ggaaagggaa ccaaccagca
      241 agagttgcta gtgggacaaa gccagccagc ataaggaaga aatgagtcag agtggcacga
      301 agcatgctcc agtcacggct aaataagcgg ttgccaaagt tgaagagcat accgatggtt
      361 gcccagataa gtgtgcagta gagcaagacc agggcaccgt gaacctgatg ctgatccatc
      421 acttggccta tgagagagta gggatttagt ggtgcgtagg tatttggtgc ataaatgagt
      481 gaaaagagga tagagaggat gaggccgata agaacaccgg tagctgcatc gtgaaatact
      541 tgttttttca tagttctaat ttctccttga tggtttttag ataacggcgt gaagagtagg
      601 tgaagctttc gtttttcaag aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag
      661 agatggaatc gatattgatg atttctgatt gggaaatttg gataaaattg cttggcaaga
      721 gtttaagaac ctgatagagt cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta
      781 gaaccttccg attctcgata tagaagcgct gaatcttgcc aatctcaact agatagacct
      841 gatcatcgat ttttcctttg attttttctc tttggtccag attttctgcg aactcgatga
      901 ctttctggac tttttcggtt tcttgaggtg cttggacaat cagcttttcc tcctcgtaag
      961 tctcactaat ctgtagttct actttcataa atttctctcc ttgtttttca tacaaaatta
     1021 atctcacttc ttgtacacct ttattaatac tcttcaaaaa tctcttcaaa ccacgtcagc
     1081 gtcgtcttgc cgtaggatag ttgttgactt tgtcagtttt atctacaacc tcaaaacagt
     1141 gttttgagct gtctgcgtct agctttctag tttgctcttt gatttttatt gagtatacca
     1201 ctattttact ccctctggca agggactttg tctatgtgga gggattgggc tcctatgtgg
     1261 tggagttttt ctgttctttc tgaaatatgg tataatagca ctaattaatt tctaggaaaa
     1321 tagatacaga aaggggctga aagatgtctc atattattga attgccagag atgctggcaa
     1381 accaaatcgc agctggggag gtcattgaac gtcctgccag tgtggttaaa gagttggtag
     1441 aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa gctggtctca
     1501 agaaggttca aatcacggat aacggtcatg gaattgccca cgatgaggtg gagttggccc
     1561 tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg attcggacgc
     1621 ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg actctgttaa
     1681 cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt gaagttgagg
     1741 aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat ctctttttca
     1801 acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct catatcattg
     1861 atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg attagtgatg
     1921 gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc gcagggattt
     1981 acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta gatttcgaaa
     2041 tttcaggttt tgtgtcttta cctgagttga ctcgggctaa ccgcaattat atcagcctct
     2101 tcatcaatgg ccgttatatc aagaacttcc tgctcaatcg tgctattttg gatggtttcg
     2161 gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat atcgaccctt
     2221 atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc aaggaaaaag
     2281 aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa accttgattc
     2341 cagatgcctt ggaaaatctt gccaaatcga ccgtgcgcaa tcgtgagaag gtggagcaaa
     2401 ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg tcaagaccta
     2461 gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag gatttgaccc
     2521 tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat tttgcagaga
     2581 gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct agcatcgata
     2641 aggcttatga caaactggag cgagaagaag catccagctt cccagagttg gagtttttcg
     2701 gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac atcatagatc
     2761 agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt ggcaatgttg
     2821 accagagtca gcagcaactc ctagtgccct atatctttga atttcctgcg gatgatgccc
     2881 tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta gcagagtacg
     2941 gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa gagattgaat
     3001 caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct atcaagaaat
     3061 accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc aatcatcgta
     3121 ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac aatccctata
     3181 actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg gaaaagatgt
     3241 tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat taaaagtata
     3301 aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt gttcaaaaac
     3361 cttgatttta tgcgttttat catggaaata gttacttcat gttttcctaa ttcttttcga
     3421 aactcttttt aaaccacgtc agttttatca gtaatctcaa aacagtgttt tgagctaatt
     3481 ttgccagttt tgtctgtaac atcgaagttg tgttttacca ctctgcgact ggtttcctag
     3541 tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc tttattcgtc
     3601 taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat ttcaatagct
     3661 ccttgtgcac actcagaacc cttatttcct gctttggtac cagctcgttc tatggcttgt
     3721 tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt taaactgatt
     3781 tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt attccctcta
     3841 atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat ttttgatgca
     3901 atcagtggta tttcaaaagc tcctggaacc caggctacct ctatatcttt ctcgtttaca
     3961 ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat aaattcatta
     4021 aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc ttcataagtg
     4081 ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt tgtttctaaa
     4141 taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga aatggtaatt
     4201 ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg aagtgactga
     4261 agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc accttcaaag
     4321 cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg ataggctttt
     4381 aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa gacacccgaa
     4441 ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc gcaacgtaaa
     4501 gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac attggcttca
     4561 tcctctatgt ttcccataat aagagcaaga tgatgttccc catttagttt atctatatag
     4621 ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac tcgttctatc
     4681 agctgatcat atacttttct atattcttgt aattctttga tggtaattag tggaatgttg
     4741 tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc atgattcatt
     4801 atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc aacagttgct
     4861 tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg aaacatgtgt
     4921 cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg tgcggtcagt
     4981 cctctttcct cggcagaaat acctgtggtc gtttctttat aatcaattga aactgtaaaa
     5041 gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa ttgattagct
     5101 aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat aaaattaaca
     5161 ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc tctatccttg
     5221 tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgtttc ttgtattttt
     5281 cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata atagttcctt
     5341 agatatttct gattttggag agttatccat cagtttttgc acatatttac ctaagatatc
     5401 attttcaaga tttactgtac tcccgacttg tttactttta agaatggttt gtttcaaggt
     5461 atgagggata acagatactg aaaagtttac tttggagact ttagcgacag tcagactaat
     5521 gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt tttgtgtgtt
     5581 gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc ctgtaccatc
     5641 aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg atagataagg ctctttctag
     5701 attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg tttcattcat
     5761 tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga taccatttac
     5821 tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa ttgatagttt
     5881 acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa ttattcctgt
     5941 gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg agaaaaagca
     6001 taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc gacaggaaac
     6061 ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc aacaatttgt
     6121 tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct atcaatctgc
     6181 atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt tttctttatg
     6241 gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc ttcagaggaa
     6301 gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt aagaggagtt
     6361 cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa tctacatgtc
     6421 agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact aacatggtgt
     6481 cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga ttgatttgtt
     6541 ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata gggtacatgc
     6601 tgggtaatat actttctaaa actttttatt aagttaagac actcattttc taaaattcca
     6661 acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga tacaatagga
     6721 ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat agcatctata
     6781 cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag cgtcgctccg
     6841 acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc accaaaaaac
     6901 tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc gaccatagga
     6961 ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt catatatttt
     7021 gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac tactcaaggc
     7081 atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt aatcatttca
     7141 gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat actgtcggct
     7201 ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt agggaattac
     7261 accctgccct gaagatagtt attcaattac agatgattat agtacttaat tttgaatatg
     7321 tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg attctcgctc
     7381 ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg aatactatgt
     7441 acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt cttgaaacca
     7501 atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt caggttaatc
     7561 aggaggatca gatttatgtg catcaggttg tgcgtgagga cgcccatttg ctttatggat
     7621 ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct gggattggtc
     7681 ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt caagccattg
     7741 aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa acagcccagc
     7801 agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatgacctt cctgccaagg
     7861 tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa gccatgttgg
     7921 ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa ggaacgacag
     7981 atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag gagcagagaa
     8041 tgacaaaacg ttgttcgtgg gtcaagatga caaacccgct ctacatcgcc tatcatgatg
     8101 aggagtgggg tcagcccctc catgatgacc aagcattgtt tgagttgttg tgtatggaaa
     8161 cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct ttccgagaag
     8221 cctttcatag ctatcaaatt cactcagtcg cagagatgac tgagactgaa ttggaagcca
     8281 tgctggagaa tccagctatc attcgaaata gagccaagct ttttgctaca cgcgctaacg
     8341 cccaagtcct tctacagtta caggcagagt acggctcttt tgatgcctat ctttggtctt
     8401 ttgttgaggg gaaaattgtc gttaacgatg ttcctgatta tcgccaagcg ccagctaaaa
     8461 cacccttatc tgagaaatta gccaaagatc tcaaaaaacg aggcttcaag ttcacaggcc
     8521 cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac gagaatgatt
     8581 gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag ggaattctga tttttgcgat
     8641 tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt taatgccatc
     8701 tgtcattgca aattatcttg tttatgtagt tttagcttta tatggctcct tcttgttcaa
     8761 ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat tcttctttgg
     8821 cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat ttgtatcaga
     8881 gatgttgaag cagtttgtgg gactagatgg acaaggtcta aatcagtcta atattcaaag
     8941 tacctttcaa gaacaaccac tactgatagc tgtttttgct tgtgtcattg gacctctggt
     9001 agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt tgccaggttt
     9061 actaagcatt attctggtag gacttgtttt tgctctgact catatgcaca gtttggctct
     9121 atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt ctattattta
     9181 tgtgaaagaa aaagagaata tctactatcc cctacttgtt cacatgttaa gcaacagcct
     9241 ctccttaatc attttagcta tcagtatagt aaaatgaaat aagaacagga caaatcgatc
     9301 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
     9361 aatttgctgt aatatgtgat gaaaatacca gtaatgatac cgagaaaaaa gctgagaaac
     9421 ttttcccagc tttatttgtt atagtcaaag cgaatgactt gttcctgtgc atctacatga
     9481 gcatggaccc caaagggtac aattgctctt ggagttgcgt ggccgacatt cagattatag
     9541 acaatcggga tattgctgtc aatgatatcc aatagtgcct ctttatagtc gtcatggaaa
     9601 gtttcatcca taggttttcc gaccaagagt ccattgatga ccgcgaatat gccagtgtcc
     9661 tttaaagtta gcaacatctt tttgaagtct tctggcttag gcttttcttc gcttgtttcg
     9721 agcaagagga ttttcccttc ccagtctgac aagtcaggga aaagtttgta tttttggcag
     9781 agttccgtgc tatctgcgta tcgagagttg tcaaagatat cgtagaggga ttcgaggcaa
     9841 ccaccgagga tttttccctc gaactgagca cttccttgca acaagtcaaa acctgtattt
     9901 gtatgactga cacgaggtgt tcccagggcc gtgggactaa aatcagttcg ttcctcatac
     9961 caaacgtcac tagggcggat ttctgaaatt cttcccgtct taatcaattc tttaaagtag
    10021 tgaaggctat aggctagcat ttctttgtct aattcacaaa tgtctgctaa aaaggattga
    10081 ccataaaaag tcttgattcc taatttatgc aacatgaggt ggttcatggt tgtatccgag
    10141 aagccaagaa aaattttttg cttgataacc ttttggagtt ggtcattttc aaaaagataa
    10201 ggtagcaagc gataggtatc gtctccaccg atggcacata ggatcatgtc gatgctatca
    10261 tcagaaaagg catgaatcaa atcctctgca cgagcttcag gatggtcctt gataaagtct
    10321 aatcctttta acgaatggga caaaaagatg ggattgagtc ccagatcctt gagacgttgg
    10381 atacccaagt ccacttcgtg tttgacaaaa tcctctccga taatgccact agataaacta
    10441 acaataccaa tagtagaaac catatctcat cctcctagaa atagattgag cctattttat
    10501 cacaaaatat aagagaaagc tatgtgggat ttcagaagaa tacttttaat actcttcgaa
    10561 aatctcttca aaccacgtca gttttatctg caacctcaaa gctgtgcttt gagcaacctg
    10621 cagctagctt cctagtttgc tctttgattt tcattgaata taactcaaaa aagtaagtga
    10681 aaaagcaacc gcacctgcag ttgcttttat cgttttctta ttcttgatag cgtgttggtc
    10741 ctgcacttgc actacggata ttcaagcgtt tcttgagata gaagcgaagg gctaggaggg
    10801 ctgctccaat aatagctagt ggcaatggat ccagtactgg gttaaggcta gctggtagga
    10861 agcttgttgc aaagaagaca agcaaccaaa ggaacataga agctaggata actagtacag
    10921 atttccagaa aggtggacgt tgactgcgat ccatatctga tccatagtat tggtaaacaa
    10981 agtagtacat caagtagaag gcaaatccac caaccagtcc aactaataga agagtaatca
    11041 atccatagcc gaaagcttgg tctgccgcaa agaaggttgt gagggcgctg acaagggcaa
    11101 agaggctagt gatgaaaaga gctgagtcca taatcatcag ttttgggtca tcattttctt
    11161 ttggatgctc tttttcgtac tgctctttga cagtgaagct atgagcccaa tgagttggtg
    11221 cgccgtatag ggaacgggca gttgtacctt tagattgctc ctcaaggatt tggggaatga
    11281 cttcctcaaa aatagtctgg atttcagcgt ctgttttccc atctttgatg aattgttggg
    11341 tagcaatgtg gacaaactct tggtttttct tagttaattt ttgtagatca atttgagaca
    11401 taggaactcc tcttagaacc attttttatg gatgagatag agagtgagcg agacactcat
    11461 agcaaaggcg ataaagacga ttaaccagaa ggcatttggc tctccgttta gggggatttc
    11521 attatcctta aagttcatcc cgtaggcaga aaagaccatg gttgggatgg acatgacgat
    11581 ggtcacaagg gccaaggttt tcatgatgtt gttctggttg ttagaaatga tagaggcaaa
    11641 ggtctctgtc atagaatgca agacgtttcc ataaatatct gccatctcga tggcctgttg
    11701 ggtttcaatc agggtgtctt caagcaggtc ttcgtcctca aggtatttct tgatattgct
    11761 ggttgaactg gtcaatttct taatcacgcg ctcatttgtt ttgagggagg ccttgaaata
    11821 gacgatagtt ttttccaatt ccatgagctc aatcaattct tcattacgag ttgattgatg
    11881 cagttgactt tcgatttgtt cactcttgcg gtcgattgaa cgaagggctg ttaggtaaag
    11941 ctctgcattg cgataaagaa tttgaaagat aaaacgtgaa cgcatgaagg tatagaaatt
    12001 acgcaatcga cggttgataa agacatcaag gacaggtagt ggttccaaac acgtagtgat
    12061 aatggtttcc tcagtgatga taataccaag cgggatggtt acgtagtagg tgcggttatt
    12121 tctttcctcc gtgaccggca cgtctacgat aatcagggta tactcgtctt caatggtaat
    12181 acgagacatt tcttccgcat cgagcggtgc tcgaaggtcg gcaatatcaa tatcgaaggt
    12241 gttggcgatt tcgagtgatt cattttgagt cggattgacg agattgatcc aagtacccgg
    12301 ttcaagcgta tcgatctctt taaattcagt tgttgtagag agaaaaactt gtttcatatc
    12361 ccttatcctt tctcattttt cagatttttt cacactgtac tattatacta caaaatcggc
    12421 cttttgggta atagaatctc actttttttg gtataatggt aagcaataat ggactagaaa
    12481 gaacaaagat gcaagataaa attgtcattc atggggcgcg tgcccataat ttaaaaaata
    12541 ttgatgtgga gattccgcga gacaagttgg ttgtcgtgac tggcttgtca ggttcaggga
    12601 agtccagtct ggcttttgat actctctatg cggagggaca acgtcgctat gtagagagtt
    12661 tgtctgccta cgctcgtcag ttcttgggaa atatggaaaa accagatgta gatgccattg
    12721 atggtctcag cccagctatt tccatcgacc agaaaacgac tagtaaaaat cctcgctcga
    12781 cggtgggaac gacgactgaa atcaatgact atctgcgtct cctctacgca cgtgtgggga
    12841 cgccttactg tatcaacgga catggagcta tcaatgcttc ttctgtggag caaatcgttg
    12901 ataaggtttt ggagttacct gaacgccagc gcttgcagat cttggctcct gtcatccgca
    12961 agaaaaaagg ccaacataag agtgttatcg agaaggttca gaaagacggg tatgttcgtg
    13021 ttcgtgtgga tggggaagtc tatgatgtga ccgaagtgcc agagttgtct aagagcaagc
    13081 aacacaatat cgatgtcgtg gttgaccgta ttgttatcaa ggagggcatt cgtagccgtc
    13141 tctttgattc cattgaagct gcccttcgta tcgcagaagg ttatgtgatt atcgacacca
    13201 tggacgactc tgagttgttg ttctctgagc attatgcctg tccagtttgt ggatttactg
    13261 tcccagagtt agaacctcgt ctcttctctt tcaatgctcc ctttggctct tgtagtgagt
    13321 gtgacggctt ggggattaag ctggaggtgg atactgattt ggtagtgcca gatgccagca
    13381 aaaccttaca tgagggagcg ctggcacctt ggaatcctat ctcatccaac tactatccaa
    13441 acatgttaga gcaggccatg aaagtctttg gagtggctat ggataagcct tttgaggacc
    13501 tgtcagaaga agataagaac ttgattctct acggctcaga tggtaaggaa ttccatttcc
    13561 attatgaaaa tgaatttggt ggtgtgcgcg atatcgacat tccgtttgag ggagttatca
    13621 ataatatcaa acgtcgttac catgaaacca atagcgatta cactcgcact cagatgcgtc
    13681 tctacatgaa tgagctgacc tgcggaacct gtcaaggcta tcgtctcaat gaccaggcct
    13741 tgtctgtccg tgtgggcggc cagcaagggc cacatattgg agaaatctca gacctgtcta
    13801 tcgctgacca cttggacttg gtgagccagt taactttgtc tgaaaatgaa gccatcattg
    13861 ctcgtcccat tctcaaggaa atcaaggatc gtttgacctt ccttaataat gtgggtctta
    13921 actatctaac cctgtcacgt tcagcaggaa ccctttcagg tggggaaagt cagcgcattc
    13981 gtttggcaac ccagattggt tccaacctat caggtgtcct ctatatccta gacgagccgt
    14041 caatcggtct tcaccagagg gacaatgacc gcctgattgc cagtctgaaa aagatgcgtg
    14101 acttgggcaa tactcttatc gtggtggaac acgacgaaga taccatgcgt gaagctgatt
    14161 atctgattga cgttggtcct ggtgccggtg tttttggtgg tgagattgtt gcagcaggta
    14221 cacctaagca agtggctcgc aacagcaagt ctatcacagg tcagtacttg tcaggcaaac
    14281 gtgtcatccc agtaccagaa gagcgccgtg tcggaaatgg tcgttttatt gaagtgacag
    14341 gagcgcgtga gaacaacttg caaaatgtca cggctcgctt tccactagga aaatttatcg
    14401 cagtgacagg tgtgtcgggt tcagggaaat cgactctaat caacagcatt ctcaaaaaag
    14461 ccattgccca gaagctcaac cgtaattcag acaaacctgg taagtttaag actattacag
    14521 ggattgagca tgtcgaccgt ttgattgata ttgaccagag ccctatcgga cggacgccga
    14581 ggtctaaccc agctacctat acaggagttt ttgacgatat acgtgacctt tttgctcaga
    14641 caaatgaagc caagattcga ggctacaaga aggggcgctt cagtttcaac gtcaagggag
    14701 gtcgctgtga agcttgctca ggtgacggga ttatcaagat tgagatgcac ttcttgccag
    14761 atgtttatgt ggcttgtgaa gtttgccacg gaacccgcta taacagtgaa accctagaag
    14821 ttcactacaa ggaaaagaat atttcgcagg tcttggatat gacggtcaac gatgcggtag
    14881 aatttttcca acacattccg aaaattcaac gcaaactcca gaccatcaag gatgtaggtc
    14941 taggctatgt gaccttggga cagccagcta ccaccctttc tgggggagaa gcccaacgta
    15001 tgaagttagc tagcgaactc cacaaacgct cgacaggaaa atctttctac attctggatg
    15061 agccgacgac agggcttcat acagaggaca ttgctcgcct gctcaaggtt ttagctcgct
    15121 ttgtagacgc cggcaataca gtcctcgtca tcgagcacaa tctggatgtt atcaagacgg
    15181 cagaccatat cattgacttg ggacctgagg gcggtgtcgg tggtggaacc atcatcgtaa
    15241 caggaactcc agaagaagta gctgccaacg aagccagcta tacaggacac tatttgaaag
    15301 gaaagttaca tcatgaataa acgcgtacaa gcatttctag ctaaaatgca agaaaaagaa
    15361 ctagatggta tcatcatcaa caatcttaaa aacgtctatt atttgactgg tttttggggc
    15421 tcaaacggaa cagtctttat cagccgtgac cgtcaggtct tagtgacaga ctctcgctat
    15481 atcatcgcag ctaagcaaga aaccagtggt tttgagattg tggctgatcg tgatgaattg
    15541 gctgtcattg caggaattgt taaggacatg aacttgactc gtatcggttt tgaagatgag
    15601 atttcagtgt cttattacca ccgtatgcag gcagcttttg caggtttgaa cttgcttcca
    15661 caaactcagt ttgtggaagg tcttcgtatg attaaggatg aggcagagat tgcagcgatt
    15721 cgcaaggctt gttctatctc agaccaagct ttccgcgatg cgcttgactt tatcaaacca
    15781 ggaaaaactg aaattgagat tgccaacttc cttgatttcc gcatgcgtga gttgggagca
    15841 tctggcttat cttttgatac gatcctagct agcggtatca attcttctaa accccatgcc
    15901 catccaatgc acaaaccagt ggagttggga gaagccatta ccatggactt cggctgtctc
    15961 tatgaccact atgtcagtga tatgacccgg actatctatc tagggcatgt tagcgatgag
    16021 caggcagaga tttacaatac ggttctaaaa gctaaccaag ccttgattga ccaagctaag
    16081 gcaggcttag gtttccgtga ctttgacaaa atccctcgtg atattatcat tgaggcaggt
    16141 tatggtgact actttactca cggcattggc cacggtattg gtctggatat ccatgaggaa
    16201 ccctacttta gtcagacttc tacagaaact attaagacag gtatggcctt gaccgatgaa
    16261 ccaggtatct atatcgaagg caaatatggc gttcgtatcg aggatgatat cctgattaca
    16321 gagacaggtt gtgaattatt gaccctagct ccaaaagagt tgatagtcat ttagttgttt
    16381 gtcaagaaaa aagtacgaaa atgacgaaaa aagtcaaaaa aaattaaaaa taggtcgcaa
    16441 gtcggatgtt ttttatggta taatagacta aactgatagt aacatgtagc gaaaggggta
    16501 ggtacatgat taaaatttat acagtctcaa gttgtactag ttgtaaaaaa gcaaaaacct
    16561 ggctcaatgc ccaccagtta agttataaag aacaaaacct tggtaaagaa ggaattacga
    16621 gagaagaatt actggatatt ctaaccaaaa cagataacgg aatagccagc atcgtttcgt
    16681 ctaaaaatcg ctatgccaaa gcccttggag tggatattga agatttgagt gtcaatgaag
    16741 ttctcaatct gattatggaa acaccgagaa ttttaaagag cccaatcctt gtagatgaaa
    16801 aacgcctgca agttggctac aaggaagacg atattcgtgc cttcctacca cgctctgtcc
    16861 gtaatgtaga aaatgcagaa gcacgtttgc gtgcagctct ataaacatca aggctgggag
    16921 cacttcccag tcttattcta ttttaatttc aaaaagaaag aagaaagaaa tgaaaaaaat
    16981 agttcttgtt agtctagctt tcctttttgt cctggttggt tgcggacaga aaaaagaaac
    17041 tggaccagct acaaaaacag aaaaagatac gcttcagtcg gcattgccag ttattgaaaa
    17101 tgctgagaag aatacagttg taactaagac tttggtcttg cccaagtcag atgatggtag
    17161 ccagcagaca caaacaatta cttacaaaga caagactttt ttgagtctag ctatccaaca
    17221 aaaacgtcca gtctctgatg agttgaagac ttatattgac caacatggag tggaggaaac
    17281 tcaaaaagct cttcttgaag cggaggagaa ggataagtct atcattgaag ctcgtaaatt
    17341 ggcaggtttc aaacttgaaa caaaactatt gagcgcaacg gaacttcaaa caacgactag
    17401 ttttgatttt caagttctgg atgtcaagaa ggcttcccag ttggaacatc tgaagaatat
    17461 tggtttggaa aatcttttga aaaatgaacc aagcaaatat atttcagata gattggcaaa
    17521 tggcgcgaca gaacaataga aaatccaaat aaatagactg cctgaattgt agaaggtaag
    17581 gaattatgct ataatggtac tattctaagg aaagagggtg ttagaatggg atttactgaa
    17641 gaaacagtac gttttaaatt ggacgattcc aataaaaaag aaattagcga aactttgaca
    17701 gatgtttatg cttcgttgaa cgataagggt tacaacccaa ttaaccaaat cgtaggttac
    17761 gtattgagtg gagaccctgc ctacgttcct cgttataata atgcacgaaa tcaaatccgt
    17821 aagtatgagc gtgatgaaat cgttgaggaa ttggttcgct actacctcaa aggacaagga
    17881 gtcgatctat aacctatgag aattatggga ttggacgtcg gttcaaaaac ggtaggggtg
    17941 gcgattagcg atccgcttgg ttttacagct caagggcttg aaatcatcca gataaatgaa
    18001 gaacaaggcc aatttggttt tgaccgcgtt aaggaattgg ttgatactta caaggtggaa
    18061 cgatttgtag tgggcttgcc taaaaacatg aacaatacaa gtggaccgcg cgtagaagct
    18121 agtcaagcct acggagcaaa gctagaagag ttttttggtt taccagtaga ctatcaggat
    18181 gaacgcttga caacagtggc tgctgagcgc atgttgattg aacaagcaga tatcagtcgc
    18241 aataagcgca agaaagtcat tgataagtta gcagctcagc tgattttaca aaattattta
    18301 gatagaaaat tttaatataa aggagaggct atgtcacacg atcataacca cgaccacgaa
    18361 gaacgtgaac taatcacact agtagatgag caaggaaatg aaaccttgtt tgaaatcctt
    18421 ttgacaattg atggaaaaga agaatttggt aaaaactatg ttcttctagt accagttaac
    18481 gcagaagaag acgaagacgg acaagttgaa atccaagctt actcattcat tgaaaatgaa
    18541 gatggaacag aaggcgaatt gcaaccaatc ccagaagacg aatggaacat gattgaagaa
    18601 gtcttcaaca gctttatgga ggagtgatac agtctgggag actgtatcag cccaactcca
    18661 gaaatgggga agagttggaa cgtccagtga acatttttac cccaactctt agaaattgag
    18721 aagagttgga acatctagtg gatgttttta gcccatattc acattcatag tagctagtta
    18781 ttagctaacc aggtaagagg tcgggacgaa aatcctggcc ttgtttttat tagtaaccat
    18841 actttaatgc gataattgat tggacttttg tcaaggagat gtgtatgttt gaagtagaag
    18901 aatggctcca tagtcggatt ggtttgaatt ttcgatcagg tttgggccga atgcagcaag
    18961 cggtggattt gttaggaaat cctgagcagt cttaccctat tatccacgta acagggacta
    19021 atgggaaagg atctaccatt gcttttatga gggaattatt tatggggcat ggcaaaaaag
    19081 ttgcgacctt tacctcccct catatcgtct ctatcaatga ccgaatctgc attaatgggc
    19141 aacctatagc agacgcagac tttatccgtt tgactgatca ggtcaaggag atggagaaaa
    19201 cgcttctgca aactcctgcc cagttgtcct tttttgaatt gctgacctta gttgcttttc
    19261 tttattttag ggagcaagag gtggatttgg ttttattaga agtgggaatt ggtggcttac
    19321 ttgacacgac caatgtggta actggagagc ttgctgtcat cacctccatt gggcttgacc
    19381 atcaagaaac cttgggtgat agtctagaag caattgcaga gcagaaagct ggtattttca
    19441 aggctggtaa aaaggcagtg attgcgaaat tgcctccaga agctagactt gtctgtcaga
    19501 aaaaagccga atctttagct gttgaccttt atcaggcagg tcaagatttt tcagtgctga
    19561 atggtgattt ttcaagctct ttactaaatc tttcacagct gaacataggc ttagaaggag
    19621 cctatcagca ggagaatgcg gccttggcgt tgcaaacttt tcttcttttt atgggagaaa
    19681 gaaaggaagc tattgatgaa caggctgtaa gaaaggcctt ggaacagacc cattgggctg
    19741 gtcgcttgga gcgtattcgc ccacagattt acttggatgg tgctcataac ctccctgcct
    19801 tgactcgctt ggttgagttt atcaaagaaa aagagcagga aggctatcga cctcaaatcc
    19861 tctttggatc cttgaaacgt aaggattatc aagggatgtt gggttatctg actgaaaatt
    19921 tacctcaagt ggaactcaaa atgaccggct ttgactatca agggtctttg gacgagacag
    19981 atgtaacagg ttaccatgta attccctctt accgagaatt tatcagcagt ttcgaagaaa
    20041 gggcagacgc tcaagacttg ctgttcgtta cagggtctct ctattttatc tcagaagtac
    20101 ggggctacct gctggaccgt gagcagataa attgaccccc ttttttgaac ttgttatact
    20161 aggggaattg ctggttagag aaaatttctc taaattggta gcagaaagga aattcatcat
    20221 gaaattaaaa agattcacac tttctcttgc ttctctagca agttttagtc tcttagtagc
    20281 ttgttcacaa agagctcaac aggttcaaca gcctgttgct cagcaggtcc aacagcctgc
    20341 tcaacagaat accaatactg caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca
    20401 aaaccaacct gttgctcaac cgaccgatat tgatgggact tatactggtc aggatgacgg
    20461 agaccgtatc actttagtgg taactggaac gactggtaca tggactgagc tcgaatctga
    20521 cggggatcag aaagtcaaac aggttacatt tgattcagca aatcaacgca tgattattgg
    20581 cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc gtcgtagatg atatggatag
    20641 agacccatcg gaccaaatcg ttttaactaa ataagactat gcaagtagaa agggctggat
    20701 ttttcagcct ttttactttt acctagaaaa taatcataaa tacttgcctt ctaccgaata
    20761 cctgcgttat actagtatca attacttgtt tttagcattc aaactatcaa ggaggggata
    20821 tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg ctttagtctt tcgattatgc
    20881 tacttgaact ttgtcttgtt tttggtctct ttctttattt aggacgcatg gctcccattt
    20941 tatggattac tgtcctcatt ctactgagta tcatcacaat catttcgata gtcaaccgta
    21001 atacgactcc tgagaataag gtaacctggt tgttagtagc ctttgtgcca gtatttggtc
    21061 ccttgctcta tctgatgttt ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga
    21121 agaagctagg ctctatgcat ttccaagaag caaatagcca gctactaaaa gagaaattaa
    21181 aagaaagtga caaggcagct tatggagtca tcaagtcctt attgagtatg gataccaatg
    21241 ctgacatcta tgatcaaact gcctctacat tttttcctaa cggagaagct atgtggaaaa
    21301 agatggtaga agatcttaaa aaggctgaga aatttatttt cttggaatat tacattatag
    21361 aagaaggttt gatgtggaat cgcatactag atatactaga gcaaaaggta gctcagggtg
    21421 tagaggttaa gatgctctat gatgatatcg gctgtatggc tactttaaca ggagattatg
    21481 cacatcgact tcgtcagctg ggcatcgagg cccataaatt caataaagtt attcctcgtt
    21541 tgacagtggc ttataataac agagatcata gaaaaatatt gattgttgat ggtcagatag
    21601 cctatactgg tggggtcaat ctggcagatg agtacattaa ccacgtcgag agatttggtt
    21661 attggaagga tagtggaatt cgcttagacg gactagcagt aaaagccctg acacgcttat
    21721 ttttgaccac ttggtacatt aatcgaggag aaattagtga ttttgatcaa tatcatttag
    21781 aaaatcattc tatcccgagt gacggtttaa ccattccata cggaagtgga cccaagccaa
    21841 tttttcgagc gcaggtaggg aaaaaagttt atcagagttt aatcaatcaa gcaacagaat
    21901 cggtctatat tacgacacct tatttgatta tagattatga tttaacagag acaatcaaaa
    21961 atgcagctat gagaggggtc gatgttcgaa ttatcacccc ttacatacca gataagaagt
    22021 tcattcagtt agtcacgaga ggagcttatc ccgaccttct ttctgctggt gttcggattt
    22081 atgagtatag tccaggtttt attcatagta agcagatgtt ggtagacgaa gattttgcgg
    22141 tggtggggac aatcaatctc gactaccgga gcttggtaca ccattatgaa aatgcagtct
    22201 tactctataa aactccttct ataagggaaa tcgcccgaga ttttcgaaat atatttgcag
    22261 attctcagga agtctatcct cattctatca aaacgagctg gtatcaaaag cttgtaaaag
    22321 aaatcgccca gctattcgcc cctatcttat aagaaatcta gaacttagga tacaaaccag
    22381 acctattttt cttgtctttt actaatagaa agaaagagga ggggaattca tgctaactaa
    22441 ggaagaagtg aatgatatga ttgagttcaa acagacacat ttacaccgct ttcgggaaat
    22501 tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc
    22561 taggactttt tgatatacta agacagataa attgaacagg agaaacgata tgagtgtgta
    22621 tggtagagta gaagaagttc ataaggagaa tcgtgaacct ctagaatacc aaatcgaaca
    22681 agaatcgcat catcgtgaat caagtcgtct tcctttggtg aaaattttac tatggagtac
    22741 gcttgtaacg gggataactc taggagtacc gctattactc gacttaatga gtgcacaaga
    22801 agtgcaggat ttttatgcag gttgggggct tcatcagaca gggaagattt acagcgacta
    22861 ttatggaagt caaggtttgc tttattattt gctgacttac gtgagtcagg gcggattttt
    22921 ctttgccatc tttgagtggt tagccttggt agcaggagga tttttccttt ttagatcagc
    22981 ggacaccttg acagagcaag gagaccaagc tggacatctg gtgactattt tttacatgct
    23041 agttacaggt cttgcttttg gtggaggcta tgcgactctt ttagcgcttc ctttcttatt
    23101 cgcagccttt agtttagttg cggcttacct aagcaatcca agccatgata agggatttgt
    23161 acggattggg ctagctttgg caggcggatt tttctttgct cccttatcat cgctcctgtt
    23221 tattgctgta gtgagtttag gcttgttggt ctttaacctt gggcatagac gctttgcgca
    23281 tgggttttat cagtttcttg cagtggcttt aggtttttca cttgtctttt atccaactgc
    23341 ctactatagt gctgcaacag gaagttttgg ggatgcgatt agtggtattc gttatcctat
    23401 tgacagtatt cgctttgatt ttacttctaa aattttagag aatatgtttt tttatggctt
    23461 gttgtccctt ggtttgggat ttgtggtttg tatcttttta ggtctctttc aatccaaacc
    23521 ctttaaacta tacgtcattt cggttcctgc aagtcttgtg gtaattttag gtttgatttt
    23581 acttttcttt tcacaagagc ctctgcacgc ttcttatttg atggtcgtct tccctgtttt
    23641 cctactttta ttggtaacca atattaagag tcaacagagg gggcgtagtg ctagaagaag
    23701 ccgaagagaa acgccagtta gcctatggag tcgtttcttc aaaggaaatc tatatctgct
    23761 agtttttggg tttgtctatc ttttgtctgt tccttttttg atgaagtttg tcctttatcc
    23821 agtaccttat caagaacgta atcgtcttgc tgatttggta aaagaggaga caaatacgga
    23881 agatgctatc tatgcatggg atgatactgc gactctttat cgtaagagtg agcgcttgtc
    23941 gccatcggcg attttgtccc cgttgcacta tacagcaact gaggaaaatc gtaataagtt
    24001 acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg gtaaatgaca aggtggtagt
    24061 ctggtctgaa gtggaaacac ttttaaaaga aaattaccaa caagtaaaga ctgattactc
    24121 agagtttaaa gtctataaaa ttaaataacc aaatcaatat cttgtgtatt tttaaaaatt
    24181 ttaggatttt taacacaaga tattgatttt tctttttaga gtggtataat actttttaga
    24241 aagaacattt tagaaaagag catgcgtatg attgcactag aagaaaaaat tacaattttg
    24301 ccaactctct tcgtcgagaa acgagatggg agacgtgttg tatttgatgt ggacaagatt
    24361 gacaaggctc tccacaaggc ggctgacaag gttatggatg tgacacccct ggttgaaaaa
    24421 cgcctcaatg atctgactga gcgaattatt acagaaattc atagtcgctt tccacaggga
    24481 attaagattt acgaaattca aaatatcgta gaacatgaac tccttgaagc caaagaatat
    24541 gcgctggctg aggagtatat tacttatcgg acacagaggg attttgagcg ctcaaaagcg
    24601 acggatatca actttagtat tcataaactt ctcaacaaag accagacagt tgtcaatgaa
    24661 aatgctaata aagacagtga tgtctttaac actcagcgtg atttgacagc agggattgtt
    24721 gggaaatcaa tcggactgca aatgcttcct aagcacgtag ccaatgccca ccaaaagggg
    24781 gatatccact atcacgattt ggactacagt ccctataccc ctatgaccaa ctgctgtttg
    24841 attgatttta agggtatgtt ggaaaatggt tttaagattg gaaatgcaga ggtagagagt
    24901 cccaagtcta tccagactgc gacagcacag atttctcaaa tcattgccaa cgttgcttct
    24961 agccagtacg gtggctgttc agctgaccgt atcgatgaaa ttttggcgcc ttatgcagag
    25021 aagaattatc aaaaacatct caaagatgca gaagagtggg tattgcctga aaaacgggaa
    25081 gattacgctt ggaagaaaac gcaaaaggac atctacgatg ccatgcaatc tcttgagtat
    25141 gaaatcaata ctctcttcac ttcaaatgga caaacacctt ttacttcgtt aggttttggt
    25201 ctgggaacca gtcgttttga acgagaaatt caaaaagcta ttttaaacat tcgcatcaag
    25261 ggtcttggtt cagaacaccg tacggctatc tttcctaaac ttattttcac tctgaaaaga
    25321 ggccttaatt tagaggaagg aactcccaac tacgacatca agcagttggc tcttgagtgt
    25381 gcaaccaaac ggatgtatcc agatgtttta tcttacgata agattgttga cttgacagga
    25441 tccttcaagg tgcctatggg ttgccgttct ttcctccaag gatggaagga tgaaaatggt
    25501 gtagaagtca attcaggtcg catgaatctg ggtgttgtga cggttaatct gcctcgtatt
    25561 gctcttgagt ctgaaggtga tatgaataag ttctgggaaa tcttcaacga gcgaatgaat
    25621 atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta aagaggcgac accagcaaat
    25681 gctcctattc tttatcagta cggtgctttt ggccatcgtc taggtaaaga agaaagtgtt
    25741 gaccagctct ttaagaatcg tcgtgctacc gtttcgctgg gctatatcgg cttgtatgaa
    25801 gtagcgacag ttttctttgg taacagctgg gaaagtaatc cagatgctaa ggaattcacg
    25861 ctagacatca ttcacgatat gaaacgccgt gtagaagagt ggtcagacca atatggctac
    25921 catttctcta tctactcaac accatccgaa agtctgacag accgtttctg tcgactagat
    25981 atagacaagt ttggctctat tcctgatatc acagacaagg aatactacac caactctttc
    26041 cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat tggactttga gaaagtctat
    26101 ccagaagcag gtgcgtcagg tggtttcatc cattattgtg agtatccagt ccttcagcaa
    26161 aatccaaagg ccttggaagc tgtctgggat tatgcttatg accgtgtagg ctatctaggc
    26221 accaatactc cgattgaccg ttgctacaag tgtgactttg aaggggattt tgaaccaact
    26281 gagagagggt ttgcttgtcc aaactgtggc aatagcgacc ctaaaacagt agatgtggtg
    26341 aagcgaactt gtggctacct aggtaatcct caagcaagac cgatggtcaa cggccgtcac
    26401 aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa cgattaaaat agctgggcat
    26461 caagtaacaa attagaaaga aatgaaatgg gaaaatatca actagacgat aaggggcgcg
    26521 cacaagtgac ccgttatcac gagaaacact ctaaaggtgg agctggtaag aaagaacgct
    26581 tgcttagctt cagagaacaa tttttaaaca agaacaagaa aaaataaaag tgagagccag
    26641 ctctcgcttt tctcatagtg ggaggtaagg atggaattac gcagaccaag attagcggat
    26701 aagaaagctg ttttagatat gatgacagag tttgaaaaga gccaatcagc ccatgatgga
    26761 ggattttggg atacagagaa ttttgtgtat gaagagtggt tggaaagcaa tcaggaacag
    26821 gaaatgggga ttaatctgcc tgaaggatgg gttcctgcaa ttcagttagt ggctttttct
    26881 gagaaaggtc aagcagttgg atttcttaat ctccggttgc gcctcagtaa ctttttacta
    26941 gaagaaggtg ggcacattgg ctactccatc cgtccatctg aaagaggcaa gggttatgca
    27001 aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa agaacatcaa gaaagctctg
    27061 gtgacctgta gcgtgaataa tcctgctagc agagcagtca ttctagcaaa tggtggaata
    27121 tttgaggatg ctcacaatgg agtcgagcgt tattggatag aggtagcgaa tgaataatcc
    27181 aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt cgtatcattg actacaaggc
    27241 ctttaacttt gtggacggcg aaggcgtgcg taactctctc tatgtatcag gctgtatgtt
    27301 tcactgcgag ggatgttata atgttgcgac ttggtctttt aatgctggca ttccctatac
    27361 agcagaatta gaagagcaga tcatggcaga tcttgctcag ccctatgttc aaggtttgac
    27421 tttgttggga ggggagcctt ttcttaatac tgggattctc ttgccacttg ttaagcggat
    27481 tcggaaggaa ttgccagaca aggacatctg gtcctggacc ggctacactt gggaagaaat
    27541 gatgttggaa actccagata aactggaatt cttgtcactg attgacattc ttgtcgatgg
    27601 aagatatgat cgaactaaga gaaatcttat gctccagttt cgaggttcat ctaaccaacg
    27661 aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta gtgatttggg acaagctcaa
    27721 tgacggaaaa gaaagctatg aacaggtgaa gagagaatga agaaaaaaga cttagtagac
    27781 caactagtct cagagatcga gacggggaaa gtcaggacac tgggaatata cggtcatgga
    27841 gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag ctttagattc tactacagta
    27901 aatttgctag aaacagatcc ctatatcacc tccggacgct atttggtagt acccaaggac
    27961 gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc atgaactgga gagtttgcag
    28021 agagatatcc ttgccttgca ggcgggtatg gatgtcttga caattgaaga accttggaag
    28081 gctagtgagg tcttgtctgg agccaaacca attttgattg tcgaagggat gtctgttggt
    28141 tttctaccca aggaactctt tgaaaaaacc atctgtttct acacggatga ggagaccgaa
    28201 ttaaagcgac gccttgctag agatacgact gtgagaaatc gcgatgcatc ctttatattg
    28261 gctagccatc agatgagacg ggagcagtat ctgcgatact ataaagaaac tgagtctaag
    28321 gcggatatct tagtggacca atcagaagat aaatttgatg tcaagaggac ttaacttatg
    28381 tatagaagaa atatagaaga aaatccctaa ttttagaaag aaaaagtagg aaaacagttt
    28441 catcccaaaa aaacgaaaaa acctaacaaa tcccttgcaa tcgcagggga tttgtgttat
    28501 tctattatgg tgctgtaaat tacagcctta gttttgatgc aagaggttgc gacacgctcg
    28561 gttgcattgc cacgcaacac cgcgtcggtt ttcttgtgga gctagcctat tatcttaaat
    28621 agacgaaaag gagaaaaaga tggcaaacaa aaaaatccgt atccgtttga aagcttacga
    28681 acaccgtacg cttgacacag cggctgcaaa aatcgtagaa tcagctactc gtacaggtgc
    28741 acaagttgcg ggtccaatcc cacttccaac tgaacgtagc ctctacacaa tcattcgtgc
    28801 gactcacaaa tacaaagact ctcgcgaaca atttgaaatg cgtacacaca aacgtttgat
    28861 cgatatcgtt aacccaactc aaaaaacagt tgatgccttg atgaaattgg atcttccaag
    28921 tggtgtaaac gtagaaatca aactttaatc taaaatataa aagagcagag gctggtgttt
    28981 caatctaatt gaacacgggc tagactcggt gtgaaaaaga taaacttcct attgtccgct
    29041 agacactgcg ttagtttcct attttcactt tgagtttgac gccctttgta tcttagactt
    29101 gagcataaaa aacgctcgtt aaaaactttt tgaataaaaa atatagaaaa ggaactattt
    29161 tctcatgaca aaaggaatct tagggaaaaa agtgggaatg actcaaatct tcactgaagc
    29221 tggcgaattg atccctgtaa cagttattga agcaactcca aacgttgttc ttcaagttaa
    29281 aactgttgaa acagacggat acaacgctat ccaagttggt ttcgatgaca aacgcgaagt
    29341 attgagcaac aaacctgcta aaggacatgt agcgaaagct aacacggctc ctaagcgctt
    29401 cattcgtgaa ttcaaaaacg ttgaaggctt ggaagttggt gctgaaatta cagttgaaac
    29461 attcgcagct ggagatgttg ttgatgtaac tggtacttct aaaggtaaag gtttccaagg
    29521 tgttatcaag cgccacggac aatcacgtgg accgatggct cacggttctc gttaccaccg
    29581 tcgtccaggt tctatgggac ctgttgcacc taaccgcgta ttcaaaggta aaaaccttgc
    29641 aggacgtatg ggtggtgacc gcgtaacaat tcaaaatctt gaagttgtac aagttgttcc
    29701 agaaaagaac gttatcctta tcaaaggtaa cgtaccaggt gctaagaaat ctcttatcac
    29761 tatcaaatca gcagttaaag ctggtaaata ataaagaaag gggaaatcag tcacaatggc
    29821 aaacgtaaca ttatttgacc aaactggtaa agaagctggc caagttgttc ttaacgatgc
    29881 agtatttggt atcgaaccaa atgaatcagt tgtgtttgat gtaatcatca gccaacgcgc
    29941 aagccttcgt caaggaacac acgctgttaa aaaccgctct gcagtatcag gtggtggacg
    30001 caaaccgtgg cgtcaaaaag gaactggacg tgctcgtcaa ggttctatcc gctcaccaca
    30061 atggcgtggt ggtggtgttg tcttcggacc aactccacgt tcatacggct acaaacttcc
    30121 acaaaaagtt cgtcgcctag ctcttaaatc agtttactct gaaaaagttg ctgaaaacaa
    30181 attcgtagct gtagacgctc tttcatttac agctccaaaa actgctgaat ttgcaaaagt
    30241 tcttgcagca ttgagcatcg attctaaagt tcttgttatc cttgaagaag gaaatgaatt
    30301 cgcagctctt tcagctcgta accttccaaa cgtgaaagtt gcaactgcta caactgcaag
    30361 tgttcttgac atcgcaaata gcgacaaact tcttgtcaca caagcagcta tctctaaaat
    30421 cgaggaggtt cttgcataat gaatttgtat gatgttatca aaaaacctgt catcactgaa
    30481 agctcaatgg ctcaacttga agcagggaaa tatgtatttg aagttgacac tcgtgcacac
    30541 aaacttttga tcaagcaagc tgttgaagct gctttcgaag gtgttaaagt tgccaatgtt
    30601 aacacaatca acgtaaaacc aaaagctaaa cgtgttggac gttacactgg ttttactaac
    30661 aaaactaaaa aagctatcat cacacttaca gctgattcta aagcaatcga gttgtttgct
    30721 gctgaagctg aataatctaa ggaggaaata tcgtgggaat tcgtgtttat aaaccaacaa
    30781 caaacggtcg ccgtaatatg acttctttgg atttcgctga aatcacaaca agcactcctg
    30841 aaaaatcatt gcttgttgca ttgaagagca aggctggtcg taacaacaac ggtcgtatca
    30901 cagttcgtca ccaaggtggt ggacacaaac gtttctaccg tttggttgac ttcaaacgta
    30961 ataaagacaa cgttgaagca gttgttaaaa caatcgagta cgatccaaac cgttctgcaa
    31021 acatcgctct tgtacactac actgacggtg tgaaagcata catcatcgct ccaaaaggtc
    31081 ttgaagtagg tcaacgtatc gtttcaggtc cagaagcaga tatcaaagtc ggaaacgctc
    31141 ttccacttgc taacatccca gttggtactt tgattcacaa catcgagttg aaaccaggtc
    31201 gtggtggtga attggtacgt gctgctggtg catctgctca agtattgggt tctgaaggta
    31261 aatacgttct tgttcgtctt caatcaggtg aagttcgtat gattcttgga acttgccgtg
    31321 ctacagttgg tgttgtcgga aacgaacaac atggacttgt aaaccttggt aaagcaggac
    31381 gtagccgttg gaaaggtatc cgcccaacag ttcgtggttc tgtaatgaac cctaacgatc
    31441 acccacacgg tggtggtgaa ggtaaagcac cagttggtcg taaagcacca tctactccat
    31501 ggggcaaacc tgctcttggt cttaaaactc gtaacaagaa agcgaaatct gacaaactta
    31561 tcgttcgtcg tcgcaacgag aaataatatt aaactagtcg cttaagcaac tagtaaatcc
    31621 gccagctcgg tagcgctcca taggagtgca agccgctgtg gtacaacatt taaaggagaa
    31681 aatataaaaa tgggacgcag tcttaaaaaa ggacctttcg tcgatgagca tttgatgaaa
    31741 aaagttgaag ctcaagctaa cgacgaaaag aaaaaagtta ttaaaacttg gtcacgtcgt
    31801 tcaacgatct tcccaagttt cattggttac actattgcag tttatgacgg acgtaaacac
    31861 gtacctgttt acatccaaga agacatggta ggccacaaac ttggtgaatt tgcaccaact
    31921 cgtacttaca aaggtcacgc tgcagacgac aagaaaacac gtagaaaata aggagaacat
    31981 aaatggcaga aattacttca gctaaagcaa tggctcgtac agtacgtgtt tcacctcgta
    32041 aatcacgtct tgttcttgat aacatccgtg gtaaaagcgt agccgatgca atcgcaatct
    32101 tgacattcac tccaaacaaa gctgctgaaa tcatcttgaa agttttgaac tcagctgtag
    32161 ctaacgctga aaacaacttt ggtttggata aagctaactt ggtagtatct gaagcattcg
    32221 caaacgaagg accaactatg aaacgtttcc gtccacgtgc gaaaggttca gcttcaccaa
    32281 tcaacaaacg tacagctcac atcactgtag ctgttgcaga aaaataagga ggtaaaatcg
    32341 tgggtcaaaa agtacatcca attggtatgc gtgtcggcat catccgtgat tgggatgcca
    32401 aatggtatgc tgaaaaagaa tacgcggatt accttcatga agatcttgca atccgtaaat
    32461 tcgttcaaaa agaacttgct gacgcagcag tttcaactat tgaaatcgaa cgcgcagtaa
    32521 acaaagttaa cgtttcactt cacactgcta aaccaggtat ggttatcggt aaaggtggtg
    32581 ctaacgttga tgcactccgt gcaaaactta acaaattgac tggaaaacaa gtacacatca
    32641 acatcatcga aatcaaacaa cctgatttgg atgctcacct tgtaggtgaa ggaattgctc
    32701 gtcaattgga gcaacgtgtt gctttccgtc gtgcacaaaa acaagcaatc caacgtgcaa
    32761 tgcgtgctgg agctaaagga atcaaaactc aagtatcagg tcgtttgaac ggtgcagata
    32821 tcgcccgtgc tgaaggatac tctgaaggaa ctgttccgct tcacacactt cgtgcagata
    32881 tcgattacgc ttgggaagaa gcagatacta catacggtaa acttggtgtt aaagtatgga
    32941 tctaccgtgg tgaagttctt ccagctcgta aaaacactaa aggaggtaaa taaccaatgt
    33001 tagtacctaa acgtgttaaa caccgtcgtg agttccgtgg aaaaatgcgc ggtgaagcaa
    33061 aaggtggaaa agaagtagca ttcggtgaat acggtcttca agctacaact agccactgga
    33121 tcactaaccg ccaaatcgaa gctgctcgta tcgccatgac tcgttacatg aaacgtggtg
    33181 gtaaagtttg gattaaaatc ttcccacaca aatcatacac tgctaaagct atcggtgtgc
    33241 gtatgggatc tggtaaaggg gcacctgaag gttgggtagc accagttaaa cgtggtaaag
    33301 tgatgttcga aatcgctggt gtatctgaag agattgcacg tgaagcgctt cgacttgcta
    33361 gccacaaatt gccagttaaa tgtaaattcg taaaacgtga agcagaataa ggagaaggca
    33421 tgaaacttaa tgaagtaaaa gaatttgtta aagaacttcg tggtctttct caagaagaac
    33481 tcgcgaagcg cgaaaacgaa ttgaaaaaag aattgtttga acttcgtttc caagctgcta
    33541 ctggtcaatt ggaacaaaca gctcgcttga aagaagttaa aaaacaaatc gctcgtatca
    33601 aaacagttca atctgaagcg aaataataga ctagggaagg agaaatttca atggaacgca
    33661 ataatcgtaa agttcttgtt ggacgtgttg tatctgacaa aatggacaag acaatcacag
    33721 ttgtagttga aacaaaacgt aaccacccag tctatggtaa acgtattaac tactctaaaa
    33781 aatacaaagc tcatgatgaa aacaatgttg ccaaagaagg cgatatcgta cgtatcatgg
    33841 aaactcgccc gctttcagct acaaaacgtt tccgtcttgt agaagttgtt gaagaagcgg
    33901 tcatcatcta atcaaacctg aaaggagaaa actgaaatga ttcaaacaga aactcgtttg
    33961 aaagtcgcag acaacagcgg tgctcgcgaa atcttgacta tcaaagttct tggtggttca
    34021 ggacgtaaat ttgcaaacat cggtgatgtt atcgtggcat ctgtaaaaca agctactcct
    34081 ggtggtgcgg ttaaaaaagg tgacgttgtt aaagcagtta tcgttcgtac taaatcaggt
    34141 gctcgtcgtg ctgatggttc atacatcaaa tttgacgaaa acgcagcagt tatcatccgt
    34201 gaagacaaaa ctcctcgcgg aacacgtatc tttggcccag ttgcacgtga attgcgtgaa
    34261 ggtggcttca tgaagatcgt gtcacttgct ccagaagtac tttaattttt aggaacaaac
    34321 tagtccccta gcttcaagct agggtgccct tatgggcgta agaaaaatca aggagaaacc
    34381 taatgtttgt aaaaaaaggc gacaaagttc gcgtaatcgc tggtaaagat aagggaacag
    34441 aagctgttgt ccttactgcc cttccaaaag taaacaaagt tatcgttgaa ggtgttaaca
    34501 ttgttaagaa acaccaacgt ccaactaacg agcttcctca aggtggtatc atcgagaaag
    34561 aagcagctat ccacgtatca aacgttcaag ttttggacaa aaatggtgta gctggtcgtg
    34621 ttggatacaa atttgtagac ggtaaaaaag ttcgctacaa caaaaaatca ggcgaagtgc
    34681 ttgattaatc acgaaggaaa ggagaagtat aatggcaaat cgtttaaaag aaaaatatct
    34741 taatgaagta gttcctgctt tgacagaaca attcaactac tcatcagtga tggctgtgcc
    34801 taaagtagat aagattgttt tgaacatggg tgttggtgaa gctgtatcaa acgctaaaag
    34861 ccttgaaaaa gctgctgaag aattggcact tatctcaggt caaaaaccac ttatcactaa
    34921 agctaaaaaa tcaatcgccg gcttccgtct tcgtgaaggt gttgcgatcg gtgcaaaagt
    34981 tacccttcgt ggtgaacgta tgtacgaatt cttggataaa ttggtatcag tttcacttcc
    35041 acgtgtacgt gactttcacg gtgttccaac aaaatcattt gatggacgcg ggaactacac
    35101 acttggtgtg aaagaacaat taatcttccc agaaatcaac ttcgatgacg ttgacaaaac
    35161 tcgtggtctt gacatcgtta tcgtaacaac tgctaacact gacgaagagt cacgtgcatt
    35221 gcttacaggc cttggaatgc cttttgcaaa ataatatagg aggtaaatct aatggctaaa
    35281 aaatcaatgg tagctagaga ggctaaacgc caaaaaattg ttgaccgtta tgctgaaaaa
    35341 cgtgctgcat taaaggcggc aggggactac gaaggtttat ctaaattacc tcgcaacgcc
    35401 tcaccgactc gtttacataa tcgttgtaga gttacggggc gcccacattc agtttaccgc
    35461 aaatttggtc tgagtcgtat cgcttttcgc gaacttgcgc ataaaggtca aattcctggt
    35521 gtaacaaaag catcttggta atttaagata tcaagggtgt caaaactcca agtaaaaata
    35581 ggaaacttga cgaagaaact aaagtttcta ggaaagttta tctttttcac acagagttta
    35641 gcccgggttc aattgggctt gccaatttga acccgagcta cagctttggc aaaaaagacc
    35701 aatttgcttt ggagcattgc ttctgcatta aattgtctat ttttgctcgt gctgttacgc
    35761 tctttgtatc atgtattaac tagcaagtgc aacttgcaaa ctactagtaa gaggagaaaa
    35821 acaaaatggt tatgactgac ccaatcgcag acttcctaac tcgtattcgt aatgctaacc
    35881 aagctaaaca cgaagtactt gaagtacctg catcaaacat caaaaaaggg attgctgaaa
    35941 tccttaaacg cgaaggtttt gtaaaaaacg ttgaaatcat cgaagatgac aaacaaggcg
    36001 tcatccgtgt atttcttaaa tacggaccaa atggtgaaaa agttatcact aacttgaaac
    36061 gtgtttctaa accaggactt cgtgtctaca aaaaacgtga agaccttcca aaagttctta
    36121 acggacttgg aattgccatc ctttcaactt ctgaaggttt gcttactgat aaagaagcac
    36181 gccaaaagaa tgttggtggt gaggttatcg cttacgtttg gtaaaatcaa gatacaaagc
    36241 tcgtaaagaa caaagcaaaa ttaggaagtt ggagaagttt gtttacaaac aagccaactt
    36301 atctattttg cacagttctt agagcgtgtt cagttcagct cttgaactaa ataagtatct
    36361 gaaccccgtg aaaactggcc gttctggcct gacaatttaa caggagaaaa taaacatgtc
    36421 acgtattggt aataaagtta tcgtgttgcc tgctggtgtt gaactcgcta acaatgacaa
    36481 cgttgtaact gtaaaaggac ctaaaggaga acttactcgt gagttctcaa aagatattga
    36541 aatccgtgtg gaaggtactg aaataactct tcaccgtcca aacgattcaa aagaaatgaa
    36601 aactatccac ggaactactc gtgccctttt gaacaacatg gttgttggtg tatcagaagg
    36661 attcaagaaa gaacttgaaa tgcgtggggt tggttaccgt gcacagcttc aaggatctaa
    36721 acttgttttg gctgttggta aatctcatcc agacgaagtt gaagctccag aaggaattac
    36781 ttttgaactt ccaaacccaa caacaatcgt tgttagcgga atttcaaaag aagtagttgg
    36841 tcaaacagct gcttacgtac gtagccttcg ttcaccagaa ccatataaag gtaaaggtat
    36901 ccgttacgtt ggtgaattcg ttcgccgtaa agaaggtaaa acaggtaaat aatgttgagt
    36961 ggttgatcat caaccaccaa cctattttcc aactttgtgc atagcacacg atttaaaact
    37021 aaagaggtga aaactgtgat ttcaaaacca gataaaaaca aactccgcca aaaacgccac
    37081 cgtcgcgttc gcggaaaact ctctggaact gctgatcgcc cacgtttgaa cgtattccgt
    37141 tctaatacag gcatctacgc tcaagtgatt gatgacgtag cgggtgtaac gctcgcaagt
    37201 gcttcaactc ttgataaaga agtttcaaaa ggaactaaaa ctgaacaagc cgttgctgtc
    37261 ggtaaactcg ttgcagaacg tgcaaacgct aaaggtattt cagaagtggt gttcgaccgc
    37321 ggtggatatc tatatcacgg acgtgtgaaa gctttggctg atgcagctcg tgaaaacgga
    37381 ttgaaattct aataggagga cactagaaaa tggcatttaa agacaatgca gttgaattag
    37441 aagaacgcgt agttgctgtc aaccgtgtta caaaagttgt taaaggtgga cgtcgtcttc
    37501 gtttcgcagc tcttgttgtt gttggtgacc acaatggtcg cgtaggattt ggtactggta
    37561 aagctcaaga agttccagaa gcaatccgta aagcagtaga tgatgctaag aaaaacttga
    37621 tcgaagttcc tatggttgga acaacaatcc cacacgaagt tctttcagaa ttcggtggag
    37681 ctaaagtatt gttgaaacct gctgtagaag gttctggagt tgccgctggt ggtgcagttc
    37741 gtgccgttgt ggaattggca ggtgtggcag atattacatc taaatcactt ggttctaaca
    37801 ctccaatcaa cattgttcgt gcaactgttg aaggtttgaa acaattgaaa cgcgctgaag
    37861 aaattgctgc ccttcgtggt atttcagttt ctgatttggc ataagaaagg ggataaaatg
    37921 gctcaaatta aaattacttt gactaagtct ccaatcggac gcattccatc acaacgtaaa
    37981 actgttgtag cacttggact tggcaaattg aacagctctg ttattaaaga agataacgct
    38041 gctatccgtg gtatgattac agcagtatct cacttagtaa cagttgaaga agtaaactaa
    38101 tgaagtttta ggggatgtgc actgtaccat cccctaaaac tagatatagt catctatgat
    38161 gacatcgtat aggcgagttg atgggggaga caaccttttc tcccttatcg gcgctagcat
    38221 tttacaaaag aggagaaaat aaaaatgaaa cttcatgaat tgaaacctgc agaaggttct
    38281 cgtaaagtac gtaaccgcgt tggtcgtggt acttcatcag gtaacggtaa aacatctggt
    38341 cgtggtcaaa aaggtcaaaa agctcgtagc ggtggcggag ttcgccttgg ttttgaaggt
    38401 ggacaaactc cattgttccg tcgtcttcca aaacgtggat tcactaacat caacgctaaa
    38461 gaatacgcaa ttgtgaacct tgaccaattg aacgtctttg aagatggtgc tgaagtaact
    38521 ccagttgttc ttatcgaagc aggaattgtt aaagctgaaa agtcaggtat taaaattctt
    38581 ggtaacggtg agttgactaa gaaattgact gtgaaagcag ctaaattctc taaatcagct
    38641 gaagaagcta tcactgctaa aggtggttca gtagaagtca tctaagagag gtgacctatg
    38701 ttttttaaat tattaagaga agctcttaaa gtcaagcagg ttcgatcaaa aattttattt
    38761 acaattttta tcgttttggt ctttcgtatc ggaactagca ttacagttcc tggtgtgaat
    38821 gccaatagct tgaatgcttt aagtggatta tccttcttaa acatgttgag cttggtgtcg
    38881 gggaatgccc taaaaaactt ttcgattttt gccctaggag ttagtcccta tatcaccgct
    38941 tctattgttg tccaactctt gcaaatggat attttaccca agtttgtaga gtggggtaaa
    39001 caaggggaag taggtcgaag aaaattgaat caagctactc gttatattgc tctagttctc
    39061 gcttttgtgc aatctatcgg gattacagct ggttttaata ccttggctgg agctcaattg
    39121 attaaaactg ctttaactcc acaagttttt ctgacgattg gtatcatctt aacagctggt
    39181 agtatgattg tcacttggtt gggtgagcaa attacagata agggatacgg aaacggtgtt
    39241 tccatgatta tctttgccgg gattgtttcc tcaattccag agatgattca gggcatctat
    39301 gtggactact ttgtgaacgt cccaagtagc cgtatcactt catctatcat tttcgtaatc
    39361 attttgatta ttactgtatt gttgattatt tactttacaa cttatgttca acaagcagaa
    39421 tacaaaattc caatccaata tactaaggtt gcacaaggtg ctccatctag ctcttacctt
    39481 ccgttaaagg taaaccctgc tggagttatc cctgttatct ttgccagttc gattactgca
    39541 gcgcctgcgg ctattcttca gtttttgagt gccacaggtc atgattgggc ttgggtaagg
    39601 gtagcacaag agatgttggc aactacttct ccaactggta ttgccatgta tgctttgttg
    39661 attattctct ttacattctt ctatacgttt gtacagatta atcctgaaaa agcagcagag
    39721 agcctacaaa agagtggtgc ctatatccat ggagttcgtc ctggtaaagg tacagaagaa
    39781 tatatgtcta aacttcttcg tcgtcttgca actgttggtt ccctcttcct tggtgtgatt
    39841 tccattttac cgattgcagc taaagatgta tttggtcttt ctgatgttgt tgcctttggt
    39901 ggaacaagtc tcttgatcat tatctctaca ggtatcgaag gaatcaagca attggaaggt
    39961 tacctattga aacgtaagta tgttggtttc atggacagaa cagaataaaa gtatttactg
    40021 aatcagtaaa tactgaggga gtggaggttt aaactctgac atttgtaaga gttggatctc
    40081 ccctcttcta ttttgttttt aaatcggggt gaaaagactt tttgcttcta tttaaaaata
    40141 aaataaggag atcaaatcat gaatcttttg attatgggct tacctggtgc aggtaaggga
    40201 actcaagcag caaaaatcgt agaacaattc catgttgcac atatctcaac aggtgatatg
    40261 ttccgcgctg caatggcaaa tcaaactgaa atgggtgttc ttgctaagtc atatattgac
    40321 aagggtgaat tggttcctga cgaagttaca aatggaatcg taaaagaacg cctttcacaa
    40381 gatgatatta aagaaacagg attcttattg gatggttacc cacgtacaat tgaacaagct
    40441 catgccttgg acaaaacatt ggctgaactt ggcattgaac tagaaggtat tatcaatatt
    40501 gaagtgaacc ctgacagcct cttggaacgt ttgagtggcc gtatcatcca ccgcgtaact
    40561 ggagaaactt tccacaaggt ctttaaccca ccagttgact ataaagaaga agattactac
    40621 caacgtgaag atgataagcc tgagacagta aaacgtcgtt tggatgttaa tattgctcaa
    40681 ggagaaccaa tcattgctca ctaccgtgcc aaaggtttgg ttcatgacat cgaaggtaat
    40741 caagatatca atgatgtctt ctcagatatt gaaaaagtat tgacaaattt gaaataaagc
    40801 gtttttcaca cttgcaaaaa tccgctacaa atgttatact gaaatagtct gacttataat
    40861 tgttgtctct gtgtctagag gcatcgaatc gaaatttatg gaggtgcttt tgcgtggcaa
    40921 aagacgatgt gattgaagtt gaaggcaaag tagttgatac aatgccgaat gcaatgttta
    40981 cggttgaact tgaaaatgga catcagattt tagcaacagt ttctggtaaa attcgtaaaa
    41041 actatattcg tattttagcg ggagatcgtg ttactgtcga aatgagtcca tatgacttga
    41101 cacgtggacg tatcacttac cgctttaaat aatcgaaaaa cttggaggga taagaaatga
    41161 aagtaagacc atcggtcaaa ccaatttgcg aatactgtaa agttattcgt cgtaatggtc
    41221 gtgttatggt aatttgccca gcaaatccaa aacacaaaca acgtcaagga taagatagaa
    41281 aggagaaaac atggctcgta ttgctggagt tgatattcca aatgacaaac gcgtagtaat
    41341 ctcattgact tatgtttatg gtatcggact tgcaacatct aagaaaattt tggctgctgc
    41401 tggaatctca gaagatgttc gtgtacgtga tcttacatca gatcaagaag atgctatccg
    41461 tcgtgaagtg gatgcaatca aagttgaagg tgaccttcgt cgtgaagtaa acttgaacat
    41521 caaacgtttg atggaaatcg gttcataccg tggtatccgt caccgtcgtg gacttcctgt
    41581 ccgtggacaa aatactaaaa acaacgctcg cactcgtaaa ggtaaagctg ttgcgattgc
    41641 tggtaagaaa aaataatata ggaggtaaaa gtcttggcta aaccaacacg taaacgtcgt
    41701 gtgaaaaaga atatcgaatc tggtattgct catattcacg ctacatttaa taacactatt
    41761 gttatgatta ctgatgtgca tggtaatgca attgcttggt catcagctgg tgctcttggt
    41821 ttcaaaggtt ctcgtaaatc tacaccattc gctgctcaaa tggcttctga agctgctgct
    41881 aaatctgcac aagaacacgg tcttaaatca gttgaagtta ctgtaaaagg tccaggttct
    41941 ggtcgtgagt cagctattcg tgcgcttgct gccgctggtc ttgaagtaac agcaattcgt
    42001 gatgtgactc cagtgccaca caatggtgct cgtcctccaa aacgtcgccg tgtataatca
    42061 tcgcattaca ctgcttttcg tttaagaggg agtaactaaa tgatcgagtt tgaaaaacca
    42121 aatataacaa aaattgatga aaataaagat tatggcaagt ttgtaatcga accacttgaa
    42181 cgtggctacg gtacaactct tggtaactct cttcgtcgtg tacttctagc ttctctacca
    42241 ggagcagctg tgacatctat caacattgat ggtgtgttac atgagtttga cacagttcca
    42301 ggtgttcgtg aagacgttat gcaaatcatt ctgaacatta aaggaattgc agtgaaatcg
    42361 tacgttgaag acgaaaaaat catcgaactg gatgttgaag gtcctgctga agtaacagct
    42421 ggtgacattt tgacagatag cgatattgaa attgtaaatc cagatcatta tctctttaca
    42481 attggtgaag gttcttctct aaaagcgact atgactgtta acagtggtcg tggatatgta
    42541 cctgctgatg aaaataaaaa ggataatgca ccagttggaa cacttgctgt agattctatt
    42601 tatacaccag ttacaaaagt caactatcaa gtggaacctg ctcgtgtagg tagcaatgat
    42661 ggtttcgaca aattaaccct tgaaatcttg acaaatggaa caattattcc agaagatgct
    42721 ttagggcttt cagcacgtat tttgacagaa catcttgatt tgtttacaaa tcttactgag
    42781 attgctaagt caactgaagt gatgaaagaa gctgatactg aatctgacga ccgtatttta
    42841 gatcgtacga ttgaggaact ggacttgtct gtgcgttcat acaactgttt aaaacgtgcc
    42901 ggtatcaata ctgtgcatga tttgacagaa aaatctgaag cagagatgat gaaagtacga
    42961 aatcttggac gcaagagttt ggaagaagtg aaactcaaac tcattgattt gggtcttgga
    43021 ttaaaagata aataaaggag gaatacatgg cttaccgtaa actaggacgc actagctcac
    43081 aacgtaaagc aatgcttcgc gatttgacaa ctgacctttt gatcaacgaa tcaatcgtga
    43141 caactgaagc tcgtgctaaa gaaatccgta aaactgttga aaaaatgatt actctaggta
    43201 aacgtggtga tttgcatgca cgtcgtcaag cagctgcttt cgtacgtaat gaaatcgcat
    43261 ctgaaaacta tgatgaagca actgataagt acacttctac tacagcactt caaaaattgt
    43321 tctcagaaat cgcacctcgt tatgctgaac gtaacggtgg atacactcgt atccttaaaa
    43381 ctgaaccacg tcgtggtgat gcagcgccaa tggcgatcat cgaattagta taaaatcatc
    43441 aattttgttg agtgttatga tgatggagtc ttgtgctctt agtctagctc tggtctaccg
    43501 ctaggatttc ggtcctagcg ggaacactca tcataagttg ggatagtaga cgcttgttta
    43561 cgaaattgtt tttttcttaa gaacaacttc gtaagcaggc gtttttgagt attttcgtta
    43621 gaattatgct atactatttg aaaagaatcc tgtttaatgt taaggtttct tattttaaga
    43681 agaattggag tttacttatg aaagccatta taactgttgt tggtaaagat aaatctggaa
    43741 ttgttgcagg tgtttctggt aaaattgcag aattaggatt gaatattgac gatatctctc
    43801 aaactgtatt ggatgaatat tttacgatga tggctgttgt atctagtgat gaaaagcaag
    43861 attttaccta tcttcgtaat gaatttgaag cttttgggca aactttgaat gtaaaaatca
    43921 atattcagag tgcagcgatt ttcgaagcta tgtataatat ctaggaggtc atcatggata
    43981 ttagacaagt tactgaaacc atcgccatga ttgaggagca aaacttcgat attagaacca
    44041 ttaccatggg gatttctctt ttggactgta tcgatccaaa tatcaatcgt gctgcggaga
    44101 aaatctatca aaaaattacg acaaaggcgg ctaatttagt agctgttggt gatgaaattg
    44161 cggctgagtt gggaattcct atcgttaata agcgtgtatc ggtgacacct atttctctga
    44221 ttggggcagc gacagatgcg acggactacg tggttctggc aaaagcgctt gataaggctg
    44281 cgaaagagat tggtgtggac tttattggtg gtttttctgc cttagtacaa aaaggttatc
    44341 aaaagggaga tgagattctc atcaattcca ttcctcgcgc tttggctgag acggataagg
    44401 tctgctcgtc agtcaatatc ggctcaacca agtctggtat taatatgacg gctgtggcag
    44461 atatgggacg aattatcaag gaaacagcaa atctttcaga tatgggagtg gccaagttgg
    44521 ttgtattcgc taatgctgtt gaggacaatc catttatggc gggtgccttt catggtgttg
    44581 gggaagcaga tgttatcatc aatgtcggag tttctggtcc tggtgtggtg aaacgtgctt
    44641 tggaaaaagt tcgtggacag agctttgatg tagtagccga aacagttaag aaaactgcct
    44701 ttaaaatcac tcgtatcggt caattggttg gtcaaatggc cagtgagaga ctgggtgtgg
    44761 agtttggtat tgtggacttg agtttggcac caacccctgc ggttggagac tctgtagcac
    44821 gtgtccttga ggaaatgggg ctagaaacag ttggcacgca tggaacgacg gctgccttgg
    44881 ccctcttgaa cgaccaagtt aaaaagggtg gagtgatggc ctgcaaccaa gtcggtggtt
    44941 tatctggtgc ctttatccct gtttctgagg atgaaggaat gattgctgca gtgcaaaatg
    45001 gctctcttaa tttagaaaaa ctagaagcta tgacggctat ctgttctgtt ggattggata
    45061 tgattgccat cccagaagat acgcctgctg aaaccattgc ggctatgatt gcggatgaag
    45121 cagcaatcgg tgttatcaac atgaaaacaa cagctgttcg tatcattccc aaaggaagag
    45181 aaggcgatat gattgagttt ggtggtctat taggaactgc acctgttatg aaggttaatg
    45241 gggcttcgtc tgtcgacttc atctctcgcg gtggacaaat cccagcacca attcatagtt
    45301 ttaaaaatta agaaaatagg agaaatttta agttctattt aagattagac gtgtatacta
    45361 taatcattaa ataaagacct cctaatatta tttgaaacag ataacactga attagtttga
    45421 atttgatttt catctaatat ctttatttaa tgaactccta aactttttca taataatctc
    45481 cttcaaaagt cgcctgtatg ggtggctttt attttatcat tcatgatata atagaagcaa
    45541 acggaggacg gaaaatggta aaagtacgat tgtatttggt acgtcatggc aagaccatgt
    45601 ttaacacgat tggtcgcgcg caaggttgga gcgatactcc cttaactgct gaaggtgaac
    45661 gagggattca agagttagga atcggtttgc gagaatctga tctacagttt gagcgcgctt
    45721 attcgagtga ttctggtcgt accattcaga ccatgggaat tatccttgaa gaacttggct
    45781 tgcaggggaa aatcccttat cgcatggaca agcgtatcag agaatggtgt ttcggtagtt
    45841 ttgatggagc ctatgatggc gatcttttca tgggcattat tcctcgtatc tttaatgtgg
    45901 accacgttca ccaattgtct tatgctgaac tggctgaggg cttggtagag gtcgatacag
    45961 ctggttgggc tgaaggctgg gaaaaactca gtggccgaat caaggaaggc tttgaaatga
    46021 ttgcaaaaga aatggaagat caaggtggag gtaacgccct tgttgtcagc catggaatga
    46081 ctattggaac cattgtttat ctgattaatg gcatgcatcc gcatggtcta gataatggta
    46141 gcgtga
//
LOCUS       DAVMMR010000008        46020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMMR010000008 DAVMMR010000000
VERSION     DAVMMR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.008"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..834
                     /locus_tag="VYS74_000440"
     CDS             <1..834
                     /locus_tag="VYS74_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV3788524.1"
                     /translation="HFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGR
                     TQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRA
                     MSRFRVQIINQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDK
                     ILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEK
                     IVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTNFVLSR
                     A"
     gene            complement(889..2430)
                     /locus_tag="VYS74_000441"
     CDS             complement(889..2430)
                     /locus_tag="VYS74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3788525.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(2432..2914)
                     /locus_tag="VYS74_000442"
     CDS             complement(2432..2914)
                     /locus_tag="VYS74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3788526.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(2914..3816)
                     /locus_tag="VYS74_000443"
     CDS             complement(2914..3816)
                     /locus_tag="VYS74_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV3788527.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(3837..4367)
                     /locus_tag="VYS74_000444"
     CDS             complement(3837..4367)
                     /locus_tag="VYS74_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV3788528.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(4525..5130)
                     /locus_tag="VYS74_000445"
     CDS             complement(4525..5130)
                     /locus_tag="VYS74_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV3788529.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(5271..6308)
                     /locus_tag="VYS74_000446"
     CDS             complement(5271..6308)
                     /locus_tag="VYS74_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3788530.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(6342..7277)
                     /locus_tag="VYS74_000447"
     CDS             complement(6342..7277)
                     /locus_tag="VYS74_000447"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV3788531.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(7830..9020)
                     /locus_tag="VYS74_000448"
     CDS             complement(7830..9020)
                     /locus_tag="VYS74_000448"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV3788532.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(9235..10578)
                     /locus_tag="VYS74_000449"
     CDS             complement(9235..10578)
                     /locus_tag="VYS74_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3788533.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(10718..11533)
                     /locus_tag="VYS74_000450"
     CDS             complement(10718..11533)
                     /locus_tag="VYS74_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV3788534.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(11585..13765)
                     /locus_tag="VYS74_000451"
     CDS             complement(11585..13765)
                     /locus_tag="VYS74_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3788535.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(14051..14977)
                     /locus_tag="VYS74_000452"
     CDS             complement(14051..14977)
                     /locus_tag="VYS74_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788536.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            complement(14970..15662)
                     /gene="ftsE"
                     /locus_tag="VYS74_000453"
     CDS             complement(14970..15662)
                     /gene="ftsE"
                     /locus_tag="VYS74_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV3788537.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(15680..16775)
                     /gene="prfB"
                     /locus_tag="VYS74_000454"
     CDS             complement(join(15680..16702,16704..16775))
                     /gene="prfB"
                     /locus_tag="VYS74_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV3788538.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(16883..17539)
                     /locus_tag="VYS74_000455"
     CDS             complement(16883..17539)
                     /locus_tag="VYS74_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3788539.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(17847..18557)
                     /locus_tag="VYS74_000456"
     CDS             complement(17847..18557)
                     /locus_tag="VYS74_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788540.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(18557..19321)
                     /locus_tag="VYS74_000457"
     CDS             complement(18557..19321)
                     /locus_tag="VYS74_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788541.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(19321..20277)
                     /locus_tag="VYS74_000458"
     CDS             complement(19321..20277)
                     /locus_tag="VYS74_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3788542.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(20281..21150)
                     /locus_tag="VYS74_000459"
     CDS             complement(20281..21150)
                     /locus_tag="VYS74_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3788543.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(21418..22578)
                     /locus_tag="VYS74_000460"
     CDS             complement(21418..22578)
                     /locus_tag="VYS74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3788544.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(22679..22927)
                     /locus_tag="VYS74_000461"
     CDS             complement(22679..22927)
                     /locus_tag="VYS74_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV3788545.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(23006..23596)
                     /gene="clpP"
                     /locus_tag="VYS74_000462"
     CDS             complement(23006..23596)
                     /gene="clpP"
                     /locus_tag="VYS74_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV3788546.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(23770..24399)
                     /gene="upp"
                     /locus_tag="VYS74_000463"
     CDS             complement(23770..24399)
                     /gene="upp"
                     /locus_tag="VYS74_000463"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV3788547.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(24488..24955)
                     /locus_tag="VYS74_000464"
     CDS             complement(24488..24955)
                     /locus_tag="VYS74_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV3788548.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(24974..25531)
                     /locus_tag="VYS74_000465"
     CDS             complement(24974..25531)
                     /locus_tag="VYS74_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3788549.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            25656..26501
                     /locus_tag="VYS74_000466"
     CDS             25656..26501
                     /locus_tag="VYS74_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3788550.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            26560..27735
                     /locus_tag="VYS74_000467"
     CDS             26560..27735
                     /locus_tag="VYS74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3788551.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            27755..28210
                     /locus_tag="VYS74_000468"
     CDS             27755..28210
                     /locus_tag="VYS74_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3788552.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(28230..28901)
                     /locus_tag="VYS74_000469"
                     /pseudo
     CDS             complement(28230..28901)
                     /locus_tag="VYS74_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819367.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            29067..30542
                     /locus_tag="VYS74_000470"
     CDS             29067..30542
                     /locus_tag="VYS74_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV3788553.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            complement(30564..31508)
                     /gene="manA"
                     /locus_tag="VYS74_000471"
     CDS             complement(30564..31508)
                     /gene="manA"
                     /locus_tag="VYS74_000471"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV3788554.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(31640..31921)
                     /locus_tag="VYS74_000472"
     CDS             complement(31640..31921)
                     /locus_tag="VYS74_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV3788555.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            32241..33242
                     /locus_tag="VYS74_000473"
                     /pseudo
     CDS             32241..33242
                     /locus_tag="VYS74_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(33359..33706)
                     /locus_tag="VYS74_000474"
     CDS             complement(33359..33706)
                     /locus_tag="VYS74_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3788556.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(33817..35592)
                     /gene="spxB"
                     /locus_tag="VYS74_000475"
     CDS             complement(33817..35592)
                     /gene="spxB"
                     /locus_tag="VYS74_000475"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV3788557.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(35799..38042)
                     /locus_tag="VYS74_000476"
     CDS             complement(35799..38042)
                     /locus_tag="VYS74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV3788558.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(38052..38423)
                     /locus_tag="VYS74_000477"
     CDS             complement(38052..38423)
                     /locus_tag="VYS74_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV3788559.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(38434..38829)
                     /locus_tag="VYS74_000478"
     CDS             complement(38434..38829)
                     /locus_tag="VYS74_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV3788560.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      39013..39111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            39195..39986
                     /gene="thiD"
                     /locus_tag="VYS74_000479"
     CDS             39195..39986
                     /gene="thiD"
                     /locus_tag="VYS74_000479"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3788561.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(40196..40828)
                     /locus_tag="VYS74_000480"
     CDS             complement(40196..40828)
                     /locus_tag="VYS74_000480"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3788562.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(40821..41624)
                     /locus_tag="VYS74_000481"
     CDS             complement(40821..41624)
                     /locus_tag="VYS74_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3788563.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(41624..42148)
                     /gene="thiW"
                     /locus_tag="VYS74_000482"
     CDS             complement(41624..42148)
                     /gene="thiW"
                     /locus_tag="VYS74_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV3788564.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSATKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(42153..42845)
                     /gene="tenA"
                     /locus_tag="VYS74_000483"
     CDS             complement(42153..42845)
                     /gene="tenA"
                     /locus_tag="VYS74_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV3788565.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(42856..43506)
                     /locus_tag="VYS74_000484"
     CDS             complement(42856..43506)
                     /locus_tag="VYS74_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV3788566.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(43508..44893)
                     /locus_tag="VYS74_000485"
     CDS             complement(43508..44893)
                     /locus_tag="VYS74_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788567.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(44894..45454)
                     /locus_tag="VYS74_000486"
     CDS             complement(44894..45454)
                     /locus_tag="VYS74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3788568.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(45717..45805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT        13571 a        10050 c         8647 g        13752 t
ORIGIN      
        1 cactttgagc atgatttttc tcggcttcca aagattatgt cttgggatga gtatgccttc
       61 actaagggaa agatgagttt cattgcgcaa gattttgata atcttaatat tatcactgtt
      121 cttgaaggca gaacacaagc tgtcatccga aatcactttc ttcgctacga tagagccgtt
      181 cgttgtcaag tgaaaatcat tacgatggat atgtttagtc cttactatga cttggctaaa
      241 cagctttttc cgtgtgctaa aatcgttcta gatcgtttcc atattatcca acatctcagc
      301 cgtgccatga gtcgttttcg tgttcaaatt ataaatcagt ttgaacgaaa atctcatgaa
      361 tacaaggcta tcaaacgtta ctggaaactc atccaacagg atagtcgtaa attgagcgat
      421 aaacgttttt atcgccctac ttttcgcatg cacttaacaa ataaagaaat tcttgacaag
      481 attttaagct attcagaaga cttgaaacac cactatcaga tctatcaact cttacttttt
      541 cactttcaga acaaagaccc tgagaaattt ttcggactca ttgaggacaa tctgaagcag
      601 gttcatccta tttttcagac tgtctttaaa acctttctaa agaacaaaga aaaaatcgtc
      661 aacgctcttc aattacctta ttccaacgca aaattggaag cgaccaataa tctcatcaaa
      721 cttatcaaac gaaacgcctt tggatttcgg aactttgaaa acttcaaaaa aaggattttt
      781 atcgctctga acatcaaaaa agaaaggacg aattttgtcc tttctcgagc ttagctgact
      841 tcaacccact acagttgaca aagagccgta tttcttttac tctcattctt aagttaaatt
      901 attaaaatcc catatttggt ctatccaacc ttcataaaag tctggctcgt ggcagaccat
      961 aaggatagat cccctgtatt ctttgagagc gcgtttgagc tcatcctttg catccacatc
     1021 caaatggttg gtcggctcgt ccagcactaa aacgttgttt tcacgattca tcaagagaca
     1081 gaaacgaacc ttggcttgct ctccccctga taatacttga atctggcttt caatatgttt
     1141 ggttgtcaaa ccacaacggg caagggctgc acggacttct gcttgattaa gggcaggaaa
     1201 ggcattccag acagcttcaa gaggagtttg gcgattacca ccttctactt cctgctcaaa
     1261 ataaccaagt tctaaataat ctccacgctc cacttcccca gcgattggcg agataatgcc
     1321 caagagactc ttcaagagag ttgtttttcc aataccatta gcaccaataa tcgcaacctt
     1381 ttgattgcgt tcgaaggtaa gatttaaagg cttagtaaga ggacggtcgt aaccaatttg
     1441 caagttcttg gcttggaaga taaagcgccc tggtgtacga gctggtttga aatcaaagga
     1501 tggttttggt ttctcacttt ggagttcgat aatatccatc ttatccaatt tcttttgacg
     1561 agacatagcc atattacgag ttgcaacacg ggctttatta cgagccacaa agtccttgag
     1621 gtctgcaatc tctttctgct ggcgttcgta ggctgcctct agctgagatt tcttcatagc
     1681 ataaacttct tggaactggt agtagtcacc agagtaacgc gtcagctgtt gattttccac
     1741 atgatagaca atattaataa cgtcattgag gaatggaata tcgtgcgaaa tgagaacaaa
     1801 ggcattctca tagttttgga gatagcgctt gagccaatca atatgctcag catccaagta
     1861 gttggtcggc tcgtccaaca gcaagatatc aggcttttca aggagaagtt ttgccaaaag
     1921 caccttggtt ctttgcccac ctgacaaaga agttacatcc gtatccatgc caaagtccat
     1981 aacaccaaga gcacgcgcta cttcgtcaat cttagcatcc aaggtataga aatcacgact
     2041 ctccagacgg tcttgaagtt ctcctacttc ttccatgaga gcatcaacat ccgcgccgtc
     2101 ttcagccatt ttcatataga ggtcattgat acgagcttca gctttgaaaa gctcatcaaa
     2161 agccgtacgg agaacatcac gcaccgactg tctttcagca aggacagagt gctgatccaa
     2221 gtaaccagcc gtcacatatt tggaccactc aacctttcct tcatctggca gcattttacc
     2281 agtcacgata ctcataaagg ttgattttcc ttcaccattg gcaccgacca ggccgatatg
     2341 ttctcccttg aggagacgga aggacacatc ttcaaaaatt gcacggtcac caaaaccgtg
     2401 actcagattt ttaacttcta aaatactcat tttaattcct tatcttgttt ttatgtaatc
     2461 gtttataaag gagccaagcc agatagccac ccaaagtatt ggtccacaaa tcatcaatct
     2521 caaagacgcg attgaaatca aagaaaaagt ccaagattaa ttgcgtacac tcgattccaa
     2581 gactcacaag aaaactaaaa agaaggacct tttttgtttt ccgcaaattt ggaaatagat
     2641 aaaggagttg gaaaatcaga ggaaaaaaca agaagacatt gaggacattt tgtaaaaaaa
     2701 tccaacataa ttgtccaatg tcactcactt cgcccagttt ccagagagaa ttgaaaggag
     2761 tcaaaagaaa aaccaggcgt ccaagatgct gaatacctgg agttcccact cccacggtag
     2821 attgttcttg aggagtaaag caaaaacaga caatgcaaat gctatagaaa atgactcccc
     2881 agaccaaaat atgattataa gtcttcttca tcattaagga tttaccgctg cgactgcctt
     2941 ctggcggtca cgtttcattg tgttagagcg caattgtcca caagctgcgt caatatctgt
     3001 accatgctct tgacgaacca cacagttgac cccttttttc ttaagcgtat catagaaagc
     3061 caacacgcgc tctttgggac tacggctata ttggtcatgc tcactaactg ggttataagg
     3121 aatcaagttt acataagaca atttcttgat gttcttgagc aattcagcca attccaaggc
     3181 ttgttctaca ccgtcgttga cttcattaag catgatatat tcaaaggtta cacgacggtt
     3241 tgttgtctca atgtagtatt caatagcagc aaagagtttt tcaatcggaa aggcacggtt
     3301 aatcttcatg atacttgaac gaagttcatt gttgggtgcg tgaagagaca cggcaagatt
     3361 gacctgaacc ccttcatcag caaagtattt ctgaaccagc atgatttgcg ctacgatttc
     3421 accgttattg aggtcacgct gcttcttaat caaaccagag gcacagaagg tacaaccgat
     3481 attacagccg acctgagtgg tcacacagac agataaacca tagtgttgac gcatgagtac
     3541 agtctcaatt aacataccgt caggcaattc aaagagatat ttgactgtac catcagcaga
     3601 ctcttgcaca atacgttgtt tcaagggatt gaccacaaac tggtcattga gcttagcaat
     3661 caaatccttg gaaaggttgg tcatttcttc aaatgactgc acacgtttac ggtagagcca
     3721 ttcccagatt tgatctgcac ggaatttctt ttctccctgc tccaataccc attcctgcat
     3781 ggtttgatgt accaaactat gaattgaggg tttcatttct tctccttatt ctctactcac
     3841 ttctgacgaa tgacaaaatg acgttgttcc ttgtcctctt tctgagaatg ctggtctttc
     3901 tgacgacgtc tatttttctt atctgcattc gactttcgtt tagtttgagt cggtttcttt
     3961 ccttttctag aaggtgtttc ttcttccgtc ttacgcattt tcttgtcaaa tgatgctcgc
     4021 ttaggggctt cattttctaa gacaaaatag gcacaaccat aactacaata ctctaaaagg
     4081 tagtcttgta aacgactgat tttttcaagt ttttcttctg ttcggtcatc cttgtaaaaa
     4141 cctcgtaggc gaagctgttc gttgctccag tcccccacga tataatcaaa cttggttaag
     4201 acttctgaaa aacgctgatt aaaagtcgtc acatcaaagg catccttgat attttcaacc
     4261 aaggaaaaag ctatcccttc cgtttcgacc ttgtccccgt gtaaatggaa ctccggacca
     4321 ggaaacttgt tatagttgta taattcaggt gcaatttctt ttcgcataga tatccttttt
     4381 ccacgattac ttaatacttt attctaccat aatttctagc agttagcacg tttctcataa
     4441 aaaatgaaaa aagtctaacg attttgtcag accagaatct tataacctaa aaaagagaag
     4501 aacaattctt ccctccaact atcattattt agcagctgcg tacaattcat ctactttatt
     4561 ccagttgatt actgaaaaga aagctttgat gtagtcagga cgcacgttgc ggtatttcac
     4621 gtagtaagca tgttcccaaa cgtccaagcc caagattggt tttttacctt ctgagattgg
     4681 tgtgtcttgg tttgctgttg aagtcacttc aagtttccct tctttgttga caaccaacca
     4741 tgcccaacct gaaccaaaac gagttgttgc tgctgcagtg aaggctgctt ggaattcttc
     4801 aaatgaacca aatgttgcat cgattgctgc tgccagttct gctgaaggag ctgttttctc
     4861 gggagtcatc aattcccaga aaagagcgtg gttcaagtgt ccgccaccat tgttgataag
     4921 tgcttgacgg atatcagctg ggatagattc tacatcagca agcaaggctt caaggtcttc
     4981 accgatttca gggtgttttt ctaaagctgc attggcattg ttgacataag tttgatggtg
     5041 tttgtcatgg tgcaaatgca ttgtttccgc atcgatgtat ggttccaaag cgtcgtatgc
     5101 atatggaagt tctggtaaga taatagccat ctgtaatacc tctttttctt tctatatgaa
     5161 aatgataacg caaacattca cttttttcaa gttttttgct tatagataag caaatcagca
     5221 agaaacccac gacaagataa tcctgacgcg ggttgtagtt tcaacaaatg tcaattgacc
     5281 tgactagcaa tctgtaagag tgccttttca aaaaggaaac ctttttcata aagacctgtc
     5341 ttaatctgat agtctgtctc aatcaaatag gaaatagctt gcttcagaaa gctcaaagaa
     5401 agtcctctcg aatctcttaa tgcaaacttg atttgataag gatttgggtt acgtcccaga
     5461 taactaccta aactacttgc aatctgcgat tctgtttggc cagactccgc caaaatcttc
     5521 acctgagtaa aagtccgaaa ttgtcccagc atgactgcaa ttagtttgat ttcatcttcc
     5581 ccttgcaagg tcaagtctct caccaaatcg cgcgcctgat ccatcttttt agtcagaata
     5641 aactgagtta aatcaaaaat attgtcctgc aaggtcttgg gaattgcgtt aacaatatcc
     5701 tcttcctcga taacagaatc cgccttatag gactgtaaaa agagaagatt tttctggatt
     5761 tcgctaaatt gaaaccccga cttgatgagg agattttcaa aagaatgatt ggtaaactgc
     5821 agaccttgtt tctgactcca cttttggaaa tactggcgca attcttgttc ttttacttct
     5881 actgcatcga aggccttggc atcacgctta agtaatttga ctaaccgtct tttgctatcc
     5941 agctttcctt ctgcaaagat tatcaacttg gttgttggag aaggattgtc aaggtattcc
     6001 tcaaatgact taagctcatc atctgtcaaa aagcgtttct tagcagtcgt gatatccata
     6061 aaataatcta atatcacgat tttctcatcc gcaaagaaag gaaggctgac caactccagt
     6121 tccacatcct tgtaaactac ttctttcata tcaaagtagg caaagttgag gtcagcagaa
     6181 tcatacccaa tctgtttcaa cacttgactc ttcatcactt caaactgacc ctgatctgtc
     6241 cctgtaaata ggctcaggct cggtaaattt gataaagtca acttctgact ttcttcaatg
     6301 gctagcatcg tctctccttt cttcagattt ttcgatttaa tttagtcaat atagcgcaat
     6361 ttcccacgga aatcttctaa gctctcgtag cctttttcca ccatgattgc tttcagttca
     6421 ttggtaatgc ggtcaaaagc actgacgcct tctttgtgaa gggtcgttcc cacctgcacc
     6481 atacttgctc cacagaggat gtgttcaaag gcatctcgac cagtcagaac gccacctgtt
     6541 ccgataattt ggatttgagg atttaaacgt tgataaaagg cgtgaacatt ggctagagca
     6601 gtcggtttga tgtattctcc accaattcca ccaaaaccat tcttaggccg aataacgaca
     6661 gattcgtctt ctatatagag gccgtttccg atagagttaa cgcagttgac aaacttgagc
     6721 ggatatttgt tgaaaatagc tgccgcttga tcaaagtgaa caatatcaaa ataaggtggc
     6781 aatttaattc caagaggttt ggtgaagtaa gcaaacactt ctgccaaaat ccggtctgtt
     6841 gtctcaaaat cataggcaat ctgaggttta cctggaacat ttggacagga aagatttagc
     6901 tcagtcagac cacgaaaatc actctcttga acttttttca aaatagtatg ggtttcctct
     6961 ggagacatgc cgaccagaga taagaagaaa gttcggttcg actctttttc ctgcaaatct
     7021 aaaagataat ccaaataata gtctaagcca ttatttggca agcccataga gttgatggaa
     7081 ccaagtggaa catcttggta gcgtggctca ggattcccct gacggaagtc caaggtcgct
     7141 gtcttagtaa caaaggttcc tgccgctgag tttttgacct cttctaactc ctctatcgtc
     7201 atacaagcca cacctgctgc attcatcaag caattgtcaa actcaaaacc agcaatttgt
     7261 gttttcgttg ataccatgat tctaatcctc cagatttctt ttattgctaa tattatacca
     7321 taattttaat tctaggttta ctagattctg tggttctatc tatttattcg gaaaagaaag
     7381 tctagtacag aagtcatgct atgaaaacag ctagtgtatt ggaagtttga acaaggcttg
     7441 ggtaacagac ttttgaatga agtccaagca acaaggagaa atctcaacca tgagtccatt
     7501 ccatgactct gttgctctag aaaaccttgc aatagtcaag tgataacgaa taaaactttg
     7561 gattgaaata tccccatgat tatctattac gaggagaaat ctcaaccttt atcattttta
     7621 aagaaatata gtaaaatgaa acaagaacag gagaaatcga tcaggacagt caaattgatt
     7681 gctaacaatg ttttagaagt tgaggtgtac tattctagtt tcaatctact atacatgtaa
     7741 atctctactt tcgcaaaatg gattttcaga acagtatccc agtatagtta aaaactataa
     7801 aaaagagagg acgttcccct cttaaaatct tattttactg cttctttcaa agcatctacc
     7861 ttatccaaac gttcccatgg aagatcaata tctgtacgtc ccatgtgacc gtaagccgat
     7921 gtttgacggt aaattggacg cttgaggtcc agcatttgga taatccctgc agggcgaagg
     7981 tcaaagattt gacgagccgc tttttcaagt tgactttcag ctactgttcc tgtaccgaaa
     8041 gtatcgatac gaacagaaac aggttgcgca acaccgatag cataggccaa ctgcacttct
     8101 gccttcttag caaggcctgc tgcaacgata ttcttggcaa tatagcgagc cgcataagag
     8161 gctgaacgat ccaccttagt cgcatcttta ccagagaagg caccaccacc atgacgagag
     8221 tagccaccat aagtatctac aataatctta cgaccagtca aacctgagtc cccttgagga
     8281 ccaccgatta caaaacgacc tgtcggattg ataaagaatt ttgtcttatc atcaagataa
     8341 gaagatggaa taacttcttt gatgaccttg ttaatcacat cttgatggat ttgttcatta
     8401 gtggcctctg gatcatgctg agtagaaata acgactgtat ctacacgtac cggacggtca
     8461 ttttcatcgt actcaactgt aacttgtgat tttgcatctg gacggagata gctaatttct
     8521 ccagacttac gaagttctgc cagacgacga accaatttat gactgagtgc aattggcaat
     8581 ggcatgagct cttctgtttc atctactgca aatccaaaca tgagcccttg gtcacctgct
     8641 ccaatcaagt ccagtggatc ttgatcagca tttccacgaa cctccaaggc ttcgttaaca
     8701 ccttgagcga tgtcaggaga ttgttccacc aaagatgggt gtactcccac cgtctcagca
     8761 gaaaatccat attctgtatt ggtataacca atctctgcaa tggtatcacg aaccacacgg
     8821 ttaatatcca cataggcatt tgtagaaatt tcaccaaaaa cgtggacaga accagtatat
     8881 acagctgttt cagcagcaac gtgcgcctct ggatcctttg ctaaaatagc atccaaaatc
     8941 gcatctgaaa tttggtctgc aatcttatcc ggatgcccct cagatacaga ttcagacgtg
     9001 aataatttac gttctgacat aaaaatgtcc ccccttaaaa aatattgtta tagagttaca
     9061 aagtactgat aggaaaactc ttcgtaataa atacctacca tcttatcggg actatataac
     9121 tttatcatta taccattttt aatcgcaatt tgcagtcttt aatgtagtgt ttcggtttag
     9181 atagttcgtt ctcataaagc aaaaagttgg agctcaatac tccaacaatt tctattaaaa
     9241 tgtttggcgt ttagctttac gctctgcacg accgcgagcg cgattttcag cacgcttggt
     9301 tttacggcgc ttttcatcaa ccgcccattg aattttcttc ttataacccg gtttgacttt
     9361 tttctttttc tttttaacca aaccaatcat ttcgatatca agtttatctt gttttttctc
     9421 acggttggca cgacgatcac ggtcataggt atcttgaaat tccccgtctt tgaccatctt
     9481 aggactaaac ttgattccca atttctccaa ctcacggata tccgagtcat cacttggctg
     9541 ataaagggta atagctgtac ctggtaggcc atttcgtcca gtacgaccaa cacgatgaac
     9601 aaaaaaagat aagtcttgcg gaatggcatc attgatgaca tggctgacac cttcaatgtc
     9661 aatcccacgc gctgctaaat ctgttgcgac aatatactca aaatccagat tttgcacctg
     9721 attcatgatt cgcttgcgtt cacgaggggc aatatctcca tggatttttg caaccttcaa
     9781 gccttgagca gtcagatatg aatgcaattc atcagcacgc gttttagtgt taacaaaaat
     9841 cattgccaaa tacggctgca tcaactgagt caactggtaa atttgagcat tcttatcatg
     9901 tcccttggtc gaaatcaacc aattatcaat ggtgtcagaa ataaccgttt tggtcttaat
     9961 tttctccata acaggatttg ataagtattt tttcaagaat ggttgcagtt tttgtgggat
    10021 agtcgctgag aagaccatga attgcaagtc ttttggaaga ctgccagcaa tcttatcaac
    10081 agtttccaag aatcccatat ccaaggtcat atctgcttca tcaacaacaa atgtcttggc
    10141 tttatgaata gctaaatcac cagatttaac caagtcgtag atacggcctg gtgttccaat
    10201 aacaatatga ggctgattgc tttccaattt ctcaatctgg cgagccttat ccgtaccacc
    10261 cacataatta accacacgaa cttcgacatc tgagtgagct gaaatctgac gcgctacttg
    10321 gtaaatttga gtagccaact cacgactcgg tgcagtaatc actgcttgta cactatcgct
    10381 agcttcatct aattgctgga aaatcggtaa caagaaagta tgagtcttac ctgaacctgt
    10441 ttttgattct cctactaggt cacgacctgc caaaacaata ggaatcaact tgtcttgcac
    10501 ctctgttgga gttgtaaatt ttaactcctt caaggcttct ctaatatagt ttttaaattg
    10561 aaatttcgta aatgacataa catcctcgat tctatctatc ttatcaatta taccatattt
    10621 tattccatta cagtagtctc acttatttag gctatttcca gtagcttctc tagtaagaaa
    10681 aggctggaat tttatagttc caacctcttt tcagttatta tttccagttt aatatagcat
    10741 tcaagccata gtgatcactc acttgtggac tcttgttacc atcaaatacg acatgtaaat
    10801 tttccaccgc taactctttg gtagtaaaga cataatcgat tcgaaggggt tcagtgttcc
    10861 ctttccagcc atcaatttca ggcggaacag tatagctacc acttttctct tgagcaactt
    10921 caaatgcgtc ttgtaagcct aatggactag ctaaaatagc ttggtaacct tcctgacctg
    10981 ctgggttgtt aaaatctcca gctagtaaaa gtggcttgtt caattttttc aagacagcct
    11041 caaatcgtgc ccattcttct tggaaacctt tatcccacca agagagatga acactggcaa
    11101 ctgctagctc cttgccatcg actacagttt cagctagggc aacacggcga gtatgatagt
    11161 ctgttggatc atccacatct gaaaccaaaa tttctctggc ttcaataggt gttttagaca
    11221 agatagccac accttcgtgg tagcggtcat agccgatatg gttataagcc caggtccagt
    11281 agtaattttt cccttgctct aacaactttt caaccaagag tctaacataa tggtcttggt
    11341 gaataggctc agctgctggc aaagcttgat aaaggtcatt aacctccacc tctgacgagg
    11401 tcatctcctg attgatttct tggaaacaaa tcaaatcata gtccttttca agaatatctt
    11461 caagcaaaat ctggaatttt tcctctgctt ctttctccat ccaactgtga gtattgagtg
    11521 ttaaaaattt cattcttttc tcccaaaata agaggttgga atacagcttc caacctcaag
    11581 tatattacaa ttctacttta gcaactgctg ttttagctgc aagagaacct gttttttcta
    11641 atttaactga tttaattgca tcaccatttg tgaagacaac tactgttgaa gtttcacgtc
    11701 ctgctgcacg gatagcatcc aagtcagctg tgacaaggag atctcctgct gcaacttttt
    11761 gtccttcagc aacatgaact gtaaatggtt taccttcaag acttactgtg tccaaaccaa
    11821 tgtgaaccaa tacttcaaga cctgcttccg tcacaatacc aaaagcatgt tttgttggga
    11881 agatgcttga cacagtacct gaaactggag atacaatgtt tccatttgca ggttctactg
    11941 caaatccatc acccatcatt ttttgagcaa atactggatc ctttacttgt tccaaagcaa
    12001 caacttgacc gtctgctact gagtaaactt cctcagtaag atctttgaag tgaacagtgt
    12061 tttgttgtgc ttcagtcatt tggcttggaa gagtttcagg aatgatttca cctgaatcaa
    12121 ggatatcttg gatatcagat ttcaaaatgt cagcttttgg accgtagata gcttgaaccc
    12181 cttgtccttt catcacaaga cccatagctc cttctgcttt ccattgctct gcatttccta
    12241 ctttatctgc atctttaaca gttacacgaa gacgagtcat acatgcatca acatcaacga
    12301 tgtttacacg tccaccaaga aggttgataa tgtttacagc ttgagagcct gctgcaactt
    12361 tcacttcgct gctggtttct tctgaacctt cagcagtttc gtagtttccg ttgcgccctg
    12421 gagttgcgta gttgaatttt tgaatcatga agtttgcgat aaagtacatg attacagcaa
    12481 agagaacagt tacccaaacg aagttaacga tatccatacc aataccagca ctgattgcaa
    12541 taggtgtacg agtcaagaac tcgattgaac cgaatgagtg catacgtagg tttacgacgt
    12601 cagccatagc gaaggcagca ccttgaacaa gtgagtaaac aagatacata ggtgttgcga
    12661 tgaacatgaa catgtattcg attggttcag taacccctgt caagaatgtt gcaagagctg
    12721 ttgcaatcat catacctttg tatttatgtt tcttgtcagc atcaacatta cggtagatag
    12781 caacaatcac acccatcaag ataccgaatg aaccgatcat ttgtccaact ttgaaacgag
    12841 ctggatgtac tgtatctaac aagtgttgat attgactagc atcagtacct ttaaggttta
    12901 caaggtctgt tacccatgca agccatagtg ggtcttgacc gaatacttga gtacctttag
    12961 ctgcaccagt taaaatgtca taagtaccac caagagctgt gtagttcatt gggatagtca
    13021 acatgtggtg aagaccaaat ggcaagagca aacgttccaa agtaccatac aagaatggtg
    13081 caagaattgg agcagtttct tgtgagttgg caatccagat accgaagtta ttgatacctg
    13141 tttgaactac tggccagaaa gcagcaagta gaattgcagc gattgctgaa cgaagaataa
    13201 ctacaaatgg tacgaaacgt ttcccgttga agaatgaaag tgcatcagga agtttacgga
    13261 agttgtagta tttgttgtaa gcagttgccc ctacaaaacc tgagataatc cctacgaata
    13321 cccccatgtt caaggctgga gcttcaagaa cactgataaa gtaatcagca actttgattg
    13381 aaccaccaaa gaaagtagtt accatagcat ctggattttt caacatatcg cctgatacac
    13441 caaagattgt accagtgata cggttaatca agatgaaggc aagaccagcg gcgaaagcac
    13501 caccagcacg ttctttagcc cagcttcctc caatggctag ggcaaacaaa atgtgaaggt
    13561 taccgataac cccccaaccg atttgctcaa gaattccacc tgtgatgaca agtggtgcaa
    13621 aggttgggtt aatcatcacg atagacttac cgattgaaat catcaaacca gcagccggca
    13681 taaccgcgat aactaccatc aaagccttac cgaatttttg ccaaaattcg aaagacaaga
    13741 catttttgaa tgtatctttc atcattcaaa tctcctttat ttttatttag gcaaacgttt
    13801 tacgaaaacg tttgcacttt ctatgattta attataccac tttaattttt tttgtaaagg
    13861 cttttataaa aaaattacac ttttttctaa aaaatttcta gtgcgccaaa tttcaagttc
    13921 aaattagcca tcaagaagtc ttctctgggg gacttctttg gaagctcttt tcttctgcgc
    13981 atctcttttc aaaaactctt gtagcaaaaa aggagatttt acaatctcct cataaaagca
    14041 gctattttac ctaaaccttc aagaatcggc gcatggatat tcctgatccc aatgaaccaa
    14101 tgaaaacccc aatcacaaat agtagggcaa tcatcaacgg actaaataaa tctggactaa
    14161 tcatggatag attttgccct accaacgatt tgttgacaga ttggtaaaca atttgataaa
    14221 caataaagac caaaacagat ggtgcgatag ctcccaataa accgataaag gctccttcta
    14281 acaagaacgg tccacggata taactgtttt tagctccgac caagcgcatg atttgaattt
    14341 cgcgactgcg ggaaataatg gtaatacgaa tggtatttga aatcaagaaa actgcgataa
    14401 aaattaacaa agcagcaatc cctagtcccc aaacacggat aaatgaagct aacttgaaga
    14461 gtctttctgt attggcaccg ccatcttgaa cctcagagac accttcaatt tttttagcat
    14521 cttcggctat agtttttaca tcatttggag tgtttgcctc tacaatatag gcatcataga
    14581 gaggattggc atctccttca aagattttcc agttatctcc cattatctcg gttaattttt
    14641 catattgttc ttctttactt gaaaaggtaa cacttttaac cgtagacatg ttcttcaaag
    14701 aatcatatac cttgtggtag tcattatttg taacagtttg accttctttt tcaattgtct
    14761 gactattatc ttccacatcc tttcggatat aaactactac acggacatta ttttcaatat
    14821 ctgtagctag tttcgctgta ttgaaaataa cagatgcaaa tattgccacc aaggtcaaag
    14881 taatcatgac tgaactgaca gcagctactg tcatccaacc atttcgtttc aaacttttta
    14941 aggcttcaaa taaatggcga aaaaatctac taatcatcgt atccatactc tccttttgat
    15001 tcgtcacgaa cgacacggcc attttcaatg gcaatgacac ggtggcgcaa ggtatttaca
    15061 atctggctat tatgagtcgc catcaaaata gttgttcctt gtaggttaat ccgttccaaa
    15121 agattcataa tttcccatga attatccgga tccagatttc ctgttggctc atcagctatc
    15181 aatactttgg gattatttac aattgcacgc gcaatcgcaa tccgctgttg ctccccacct
    15241 gagagttcat ttgggaaaga acgaacctta tgcttcaatc caaccaagtc caaaacttcc
    15301 atcactcgtc ttttgatatt acggcgattt tccccgatta cttccatagc gtaagcaata
    15361 ttttcataga cagttttctt tggtaacaat ttataatcct ggaagacaac cccaacacta
    15421 cgacgtagaa gcgggatatc tttctttttg atcttaacca gattaaaacc agcaactgat
    15481 aggcttcctt tatcgatttt tacttcacga tacagagaac gaataaaagt tgacttccct
    15541 gctcctgaag gtcctacgat gtaagcaaat tcccccggtt gaacgctaac cgaaacaccg
    15601 cgtagagcag tcgttccgtt gtcgtatttt ttaacgacat ctctcatttc aataattgac
    15661 atgtgagttc ctttctatct tagctaattc gccacttgag ataagcatcg ataaaaccat
    15721 ctaagtcccc atccataacc ttatctacct gagcaacctc aaagctagtt cggtgatctt
    15781 ttaccatagt ataaggcgtg aagacataag aacggatttg gcttccccaa gtgatctcct
    15841 ttttctcacc tttgagagaa tctacctctg cagccttctt ctcttgctcc atttgataga
    15901 gcttagcctg caacatcttc atggcacgat ctctatttcc atactgggta cgatccactg
    15961 ttgattggac aacaattcca gttggaatgt gggttaaacg tacacctgtt gaaaccttat
    16021 tgacgttttg tccaccggca ccacctgaac ggaaggtatc catcttgata tcatcttcac
    16081 ggatttccac ttcaatagta tcatccaatt ctggcatcac ttctacagat gtgaaagagg
    16141 tatggcgacg tttggcagag tcaaatggtg agattcgcac taagcggtga acacccattt
    16201 ctgacttgag gagaccatag gcattaggcc cttcaaatga taaagttacc gacttaatac
    16261 cagcctcatc acctgcttgg taatccaaca cttccacttt aaagccttta gcattaccat
    16321 aacgagtata catacgaagc aacatatcac cccagtcctg cgcctcagta ccaccagaac
    16381 ctggatggat ttccaagatg gcattgttgt ggtcataagg ttctgacaag agtagagtca
    16441 tctcgtagct ggtcattatc ttatcaagtt cggctaactg cgctaccagt tcatcatgca
    16501 ctgactcgtc ttcagccaaa aaatccaata aaatttcgac ttcatcctgc aactcttcca
    16561 tcttatggaa ggtattgtaa gtgtttttta attcatttaa ttcttgcgac gttttttggg
    16621 ctgcaatatt atcgttccaa aaatcaggtt ctgtcatctt gttttccaag atggcaatct
    16681 cttcctctag cccttcgagg tcaaagagac cccctgaaag aagctaattt ttcacgattt
    16741 gcgtcaattt tctgacgaat tactgaaatg tccataaata ctcctttttt acattatttc
    16801 attatagcat attttatcat ttctttccat ctttttctct aatccccttt tcatgaaaaa
    16861 agaagccttt gggcttccct tcttacaaga cttttgctga agtgcgagcg atttcttcca
    16921 cttgaatacc atttgcttca aatttttctt tcaaggctgg taaatcaatt gatccatcga
    16981 tttgcacttc gataatcacc ttaccatcct tacgcggaat attgactgta tgggagatat
    17041 tcaaattttc ttctacaatc aaagaaacaa tttttccaag aacaccaact tcatcttctg
    17101 taacaaagcg tacacgaatc ccttcttcgc cataacctgc aatttcaaga aaggcttgga
    17161 aaacgtcacg gtcagtaata actccgtata cttgatggtt atctacgaca gggagaatac
    17221 taatcttatt tttcaacatc agataagttg catcttctag actagcatag cctgagacag
    17281 tgacaacatc gcgaatcatg acatctttta cttttgtctt attcagaaga taattcatct
    17341 catagataga aagacttgtt gctttagatg gacttgcttg tgcaatggtt ccctcagtca
    17401 ccaaaccaac taattgatca ttttcgataa caggcagacg gtgcaaacct tgctctctca
    17461 tcaaatctgc tgcatgagat actgttgtat ctggactaat ataaactacc ttgcgggtca
    17521 taaaatcttt aactgccatg agacttctcc ttttctattc ctatccctat tatacaatct
    17581 ttttgtaaat ctatcaaacg cttacatttc tttttcgaaa ttcagaaaag tatgaataag
    17641 ctagcaaaaa gagctctgaa atcgagctct gtgaatgaag gtaagaaatt tttcattcga
    17701 ttttttcaat tattagaaat taagctttca atgtagatgc agatttgttt gcaatctcaa
    17761 tctaccgact aaaaaggtcc cccggactat attgatgatt acttcgtaat cttcatctgc
    17821 cgactaaaac aatccactgg attgttttag ccacctagat atgcttttct gacttcttct
    17881 gatgaggcga gttcttttcc tgttcctgat aggacgattt tccctgtttc cagtacatat
    17941 cctcggtcag agattgcaag tgctttattg gcattttgtt caatcaagag gacggttgtt
    18001 ccttgcttct gaatatcttg aatgatatca aaaatctctt ggatgaagat tggggcaagt
    18061 cccattgatg gttcatctaa aagaagaagt tttggtgttg acataagagc gcgtcccatg
    18121 gcaagcattt gttgttcccc tcctgaaaga gtagctgcat cttggttctt acgttcttca
    18181 agacgaggaa agcgtgagaa aaccttcttc aagttagctt gattttcttc acgatttttc
    18241 tttaagaaag ctcccatttc aagattttcc ataacagtca agccaggaaa gacgtggcgt
    18301 ccttctggaa cttgtgaaag accacctgcc acaattttct gagctggcat tttttggatt
    18361 tcttgaccta aaaattcaat ctttcctgaa cttggtcgaa ccaaacctga caaggtgcga
    18421 agaatagttg tcttacctgc accgttggca ccgataaggg aaacaacttc tccttcatta
    18481 acttcaaagc ttacatcacg gactgcttgg atcataccgt aatgcacaga aagattttca
    18541 acttttaaca tagacattag gcttcacctc ctagataagc ttcgataacg cgtttattgg
    18601 tcttaatttc gtctggagtt ccttgagcaa ttaaacggcc atattcaagt acgtagatac
    18661 gttctgttac ttccatgacc agattcatat cgtgttcaat caacatgatt gtaatcttaa
    18721 attcatcttt gatacgacga attaactcag tcaattcggc tgtttcctgt gggttcatac
    18781 ctgctgctgg ttcatctaag aagagaattt taggttccgt agcaagggca cgaacaattt
    18841 ccaaacgacg ttgttgtccg taggaaagat ttttagcaag agtctctgca tcaccatcta
    18901 aatcaaagat tttcaacaat tccaaagctt tagcctttaa ttctttttca ctcttgtaaa
    18961 aagctggtaa gcgtaagaaa ctagtaaaaa catgttgttt gtgatggttt ccaaaagcaa
    19021 tcaaaacatt gtctaaaact gttaaatctt taaagagacg gatattttgg aaagtacgtc
    19081 caagtcccaa agaggcaatc ttataaggtg atttcccatt caaaaggtga ccatctaggg
    19141 ttactgttcc ctcgcttggt tcataaacac cggtcaaaag gttgaaaaga gtggttttcc
    19201 cagctccgtt tggaccgatt aatccaacca gttccccttc gttcaattca agagtcacat
    19261 ctccaacagc tgttagacca ccaaaatgtt tggttaactg ttttacttca agtaatgcca
    19321 ttagttttgt tcctccttct tagatttttt aaagaaacgt gataggctca gttcccatgt
    19381 tccaaggagt ccacctggtc tgaaaatcat taccaatacc aaggccaaag cgtaaataat
    19441 catacgcaca ctagcaacat cttggagaag catattcaaa attcccagaa caatagccga
    19501 aacaatcgca cctgtaatgg aaccgagtcc accaaataca acaataatca aaacgttgat
    19561 tgagttgatg aaggtgtaat ctttcggtac aacagacccg ataaatcctg cctgaagtga
    19621 cccagcaata cttgcagtaa tggcaccaaa gacaaaagcg atgattttaa ttttagtcgt
    19681 attaacccca actgactcag cagcgatttc atcttcacga acagagaggg ttgaacgacc
    19741 aattgggcta cgcaagaagt tcaaggttgc aatggttgta atcacgacaa agaagtaaac
    19801 catttgccaa gttgtaaagt taggaatccc taagatacct gccgcaccat ttgtaaggct
    19861 tccaccattg atgataaaga tacggataat ttcagaaaca cccagagttg ctaccgcaag
    19921 atagtccccc ttcaagcgca aggttggaat gccgacaagt aaggcaactg ctcctgaaag
    19981 caaagcccct acaagcatag ctccaaagaa ggcaccgtag gttggtaatt tagaaccaat
    20041 aatagctgct gcataggcac caatcgccat gaaaccagca tgaccaagtg aaaattgtcc
    20101 tgaaaaacca acgattaagt tgagaccaac agccaaaata atattaattc caatttgctg
    20161 taaaatctgt acatagaata gattaagtac tccgactgaa accagtacac taatcaagct
    20221 atagccagct aacaaaagga gtaaccatag aatattaact tttaaatttt ccttcatcgt
    20281 ttacaccttc tctttcacat tcttaccaag gataccagct gggcggacaa tcaagatcaa
    20341 caacaagatt ccataaacaa tggcatcacg gaaatctgac atcccaaagg cagtcgcaaa
    20401 ggtttccaat agaccaatca caaagccacc aagagccgca ccaggaataa ttccgatacc
    20461 accaagtact gcggcaacga aagatttaag acctggagta acccccatca aaggctcaag
    20521 agagttataa taaagagcaa tcagaacacc agccgcaccc gcaagagcag aacccaaagc
    20581 gaaggtaaag ctaatcgtac ggtttacatt gatccccatc aattgcgccg cgtcgctatc
    20641 tactgatact gcacgcatgg ctttccccat cttagtcttt tggacaatga cttgtaacaa
    20701 aatcatcaaa atcaaggaaa tagccaaaat cattaactgc acatttgtta agctaattgg
    20761 tcccaaatca tatcgaactg tttgaatcgc ttgagggaag gcacgggtat tggcaccaac
    20821 cagatagacc attccatact ccaataggaa agaaacccca atagccgtaa tcaaaacagc
    20881 aatacgagta gagtggcgca aaggtcggta agcaagaaac tcaatcacga caccaagaat
    20941 agctgtcgct agcatagcta caataagcgc tacaaagaaa ttcatttgga aagaattgat
    21001 caagaaataa ccgataaagg ctcccatcat ataaatatca ccatgggcga agttgatgag
    21061 cttgataatt ccgtaaacca tggtatatcc tagggctaac agcgcgtaaa cactacctag
    21121 aatcaaacca tttacgagtt gttggagcat aagattcact ctttctattt ataattccga
    21181 ggggtttccc tcactttttg ataggttctt atactcaatg aaaatcaaag agcaaactag
    21241 gaaactagcc gcaggttgct caaagcactg ctttgaggtt gtagataaga ctgacgaagt
    21301 cagtcacata tataatccaa ggcgacgttg acgcagtttg aagagatttt cgaagagtat
    21361 taaatatcga aacagggagt gagtcaaagg ctcattccct atttcaacat ttttctatta
    21421 tggttttaca acttctgctg cttcaacttt accattgttc atggtcatca tgtaagcagt
    21481 tttgactgtg ttgtggtctg catcgaagct tgtttgacca gttacacctt caaaatcttt
    21541 tgttttagca aggttatcct tgatttcacc tgaattttta gcaccttttg ctgcgtttgc
    21601 tacaaggtga actgaatcat aagccaaggc tgcaaatgtt gaaggctctt cattgtactt
    21661 agcacggtaa gcgtcaagga aggctttagc tttagctgaa acttctacag tagttgagaa
    21721 gcctgagata aagtagatgt ttgatgcttt ttcagcagtt gcttgttgta caaactcctc
    21781 accgttgaat ccatcaccac caacgattgg tttgtcaatt cccataccac gcgcttggtt
    21841 tacaatctta ccagcctcat tatagtaacc aggaacaacg atagcatcaa agtctttccc
    21901 tttcattttt gtaagggctg cttggaagtc tgtgtcacct gctacgaaag tttcatctgc
    21961 aacgatttca cccttgtatg actcgcggaa agatttggca atccctttag catagtcact
    22021 ggcattgtca gtgtaaagaa caactttctt agcatttaat ttttcagaaa catagtttga
    22081 gataattttt ccttggaagc tatcttggaa agttccaata aagaggtaat cttgaccttt
    22141 agtcaatcca tcttgagtcg cacttggtga gatcaaggga acacctgctt ttgtagcgtt
    22201 cgctaccgca gctgcagtcg caccagatgt tgcaggtcct acgactgctg atactttaga
    22261 ttgggttaca aggttagttg taactgaagc agcctcagct gtttcagact tattatcttt
    22321 atcgactact tcgatttgtt ttccatcgat accacctgcg gcattgattt catcaacagc
    22381 caattgggca cctttttgtt cagctgttcc gtatgcagct aaagaacctg attcttcaaa
    22441 gttaaacccg attttaattg tcttttcctc tactgagtta ccagcagtgt tgactgctcc
    22501 agacttcact tctccacagg ctgcaagaag tgctacactt gcaagcgcca caaacgatag
    22561 ggcaaatttt ttcttcatct ttttgtctcc ttatttctta aatagtctgt taagaatata
    22621 ccgaattatc agaaaattgt caatgttttc cttgaacttt ttcgatgata acgttttcct
    22681 atcgataaag attgcccaca aagggtgttt ccaactcttg gatatgacaa accctaactt
    22741 ttttaataaa tttttccttg ctcaagcgtc cgaccaattg ctccacttct tgagttggaa
    22801 catagagttg taagtaacga tgtttcttgg aatgataggt aatatctcca taatcctgca
    22861 gttttttggc atcacgatta tagtaaagat agataatcaa gccagagcga ttgacttttt
    22921 caaacatgat aaatcctctt tctaagaaat ctctccctat tataccaaaa aaagcaaacc
    22981 cagtcgagtt tgccttctat catcattagt tcaatgaatt gttggccata atttcatcaa
    23041 taaagccata ttcaagtgtt tcctgggcgc tcatccagtt atcacgttct gcatctgcat
    23101 ggactttttc cattgactga cctgaatttt cagccaagat tttttccaag gtattacgag
    23161 ttttgagcaa gtgttctgca gcgatagcca tatcagtttg ttgggtacca ccacctgtac
    23221 cgcccattgg ttggtgaatc atgtattcag catttggaag catgaaacgt ttgccttttg
    23281 ctccacttga tgcgatgact gttcccatag atgcagccat tcccataaca atggtttgga
    23341 catctgcctt gataaagttc atggtatcta cgattgccaa accagctgaa acagaaccac
    23401 ctggtgtatt gacataaagg taaatatctt ttgtactatc ttgggcatcc aagaaaagca
    23461 attgggcaat aacagagtta gccatattgt cttcaaccgg acctgtcagc ataatgatgc
    23521 ggtctttgag aagacgtgag taaatatcgt aagaacgttc tccacggctt gtttgttcaa
    23581 taactacagg aatcattcat ttctcctttt gagttttaat tttgttggtc aaatgactga
    23641 agataagact attataatat cttggtcaaa aaaggtcaaa tttttgctct gctttcatta
    23701 gacagaaaca aaaacccaac ctcctttcgt gactggaaat acttttccaa gtcattcttc
    23761 ttttcgatct tattttgtac cgaacaagcg gtctccagca tctccaagac ctggaacgat
    23821 ataaccgtgt tcgttcaaac gttcatccaa ggctgctgta aagatttcta catctggatg
    23881 agcttcttga agggctttta caccctctgg agcagataca aggcagacaa atttgatatt
    23941 tgatgcgcca cgttttttaa gagaatcaac agccaagatt gctgagccac ctgttgccaa
    24001 cattgggtct actataaaaa tttgacgttg gtcaatgtcc tcaggcaatt tcaccaagta
    24061 ttcaactggt tgaagtgttt cttcatcacg gtacataccg atgtggccaa ctttagcagc
    24121 tggaaccaag ctcaagagac catcaaccat cccgatacct gcacgcaaga ttgggacgat
    24181 ggccaatttc ttacctgcca attgtttttg aactgttttt gtaattggtg tttcgatttc
    24241 cacatcttct agtggaagat cacgaagtac ttcatacccc atcaacattg caatctcatc
    24301 tactagctca cgaaaagctt ttgtagaagt atctgtacga cgcaagattg acaatttgtg
    24361 ttgaatcagt gggtgattaa taacttcaat ttttcccatt tttggaattc cttctttcaa
    24421 tttattcttc ttattatacc aaaaaacggt ttaaaaatct ttctaagcca tttatttttg
    24481 ataattttta cattagatca gcctctttaa gagctgtctg tactgtctca agtggtaaat
    24541 gggtcaattc tgtccctttt tcttgataaa ggtattgggc gtagtcgtcc attcggtact
    24601 ggttgatata aaccacgcgc ttgcagccga cctgaagcaa ttgttttgta cagttgagac
    24661 aaggaaaatg ggttacatag gctgtaaagc ctttgggaac accacgctca gcaccttgaa
    24721 ggatagcatt gacctcagcg tgaagggtgc gaacacagtg gccttcaatg accaaacatt
    24781 cgtgatcaat acaatgctca gtccctgaca ccgaaccatt gtaaccagtg gaaataacct
    24841 tattatcttt taccagaatc gcgcccactt tagcacgttt acaagtggaa cgattcgcaa
    24901 ttagtagggc ttgggctgca aaatactcat cccaggccag tcttttttca gtcatctctt
    24961 ttctcctttt tctctatttt ttaaaaaatg gtaaacctaa atctgcaatc ttttcagctg
    25021 gtaccttcat gccatccttg atccatttta gaaggacaga gacgatggct gagctccaga
    25081 aggaatgaag ataagagctg acaccttttg atttcccatg gtatttttct agaaattcct
    25141 gcatggcttg gacaaagatt ttttccagat ggtaatccaa ggccaattga atcactctag
    25201 cttcctttct ggcctcccgg aaaaggtgaa cccaaaccaa ataaaggtct gtctttaaat
    25261 cgtaatgatg cagctgttcc ataatattgt ggacagttcg tttaaagacg ctctctaaaa
    25321 tttcctcttt ggagtcataa ttgcgataaa aggccgcacg cgaaacacct gcacgtttga
    25381 ccaattcaga aatactaatc ttggtcagct cctttttttc caagagttgc aagagggctg
    25441 tttcaatggc ttctctggtt aataaattgg attcttggtt tgattttctg agattttcaa
    25501 gagacttttc agagattcta cgttcagaca taacattttc tttctacttg tcacaacaga
    25561 cggatgatgc ttttgtttca agggttttca tgatataatt gtaaaaaaag acagaacctt
    25621 tgtcaatgta taactgacaa agatggagaa tttctatgac ttggaaaatt attgctgact
    25681 ctggttgtga ttatcgtcaa ctgccaacac cagctattaa cacaaccttt gtaagtgtcc
    25741 ccttaaccat tcaagtagct gatcaggtct ttgttgatga cgccagtctc gatattgacc
    25801 aaatgatgga aaccatgtat gcaactgcag aagcttcaaa atcggcttgt ccaagcccag
    25861 atgactattt gcgagcattt gaaggtgcca aaaacatttt cctagtaacc atcacaggta
    25921 ccctttctgg cagtcacaat agtgctcaac tagcaaagaa tatttatcta gaagaccatc
    25981 ctgacactaa gattcatgtg attgatagtt tatctgctgg tggtgaagtt gacctactcg
    26041 tagaaaaatt gaatgacttg attgaccagg gcttgtcttt tgaagaagtg gttgaagcta
    26101 tcactgccta tcaagaaaaa actaagttgc tctttgtcct agccaaagtc gataacttgg
    26161 tgaagaacgg ccgtttgagc aagcttatcg gtacggtcgt tggccttctc aacattcgta
    26221 tggtcggaaa agctagtgaa actggaactc tcgaattgct acaaaaagca aggggatcaa
    26281 agaaatcagt tcaagctgcc tatgatgagt tagtaaaagc tggatatgct ggtggccgta
    26341 ttgtcatggc ccaacgcaat aacgaaaaat gttgtcaaca gctctcagag cgaatccgtg
    26401 aaaccttccc acaagcggat attaaaattc taccaacctc tggtctctgc agtttctatg
    26461 cagaagaggg cggtttgctg atgggctatg aaattgatta attgcattct tgacaagagg
    26521 tgaccatcat ctcttgtttt tttgtggtac aatagagcta tgaaacattt tgatactatt
    26581 gtcatcggtg ggggacctgc tggtatgatg gctacgattt ccagtagctt ttatggacag
    26641 aaaaccctcc tcatcgaaaa aaatcggaaa cttggaaaaa aattagctgg gactggtggg
    26701 ggacgttgca atgtgaccaa caatggtagc ttagacaacc tgctagctgg aattcctgga
    26761 aacggacgct ttctttacag tgttttctcc cagttcgata atcatgacat catcaacttt
    26821 tttacagaaa atggtgttaa acttaaggtc gaagaccacg gacgcgtctt tccagccagt
    26881 gacaagtctc ggactattat cgaagctttg gaaaagaaaa tcaccgaact aggtggtcaa
    26941 gttgctactc aaatagaaat cgtttctgtt aaaaaagtag atgaccagtt tgtccttaag
    27001 tcagcggatc aaaccttcac ttgtgagaaa ctcattgtca caacaggtgg taagtcttat
    27061 ccttcgactg gttcgactgg ttttggtcac gagattgctc gccattttaa gcataccatc
    27121 accgatcttg aggctgctga aagtccttta ttaacagatt ttccacataa agccttacaa
    27181 gggatttctc tggacgatgt gaccctaagt tatggtaagc atgtcatcac tcatgattta
    27241 ctctttaccc actttggttt gtcaggtcct gctgccctac gcatgtctag ctttgtcaaa
    27301 ggtggggagg ttctctcact cgatgttttg cctcaacttt ctgagaagga cttggttaca
    27361 tttctagaag aaaatcggga aaaatccttg aaaaacgctt taaaaacctt gttaccagaa
    27421 cgcttggccg aattttttgt acaaggatat cctgaaaaag tcaaacaact gactgaaaag
    27481 gaacgagaac aacttgtcca gtccattaaa gaacttaaaa ttcctgtaac tggaaaaatg
    27541 tcccttgcaa agtcctttgt taccaagggt ggagtcagtc tcaaggaaat caatcctaaa
    27601 acccttgaaa gtaagctggt acctggcctc cactttgcag gcgaagttat ggatatcaat
    27661 gcccacacgg gtggctttaa catcacttct gccctctgta ccggctgggt ggcgggaagt
    27721 ctgcattatg attaaagatt aaaggttgtg aagaatgaac agaagagagg cagtcgaatt
    27781 tgttaacatg tgtatgatta aaaacggaga taaggtcctg gtccaagacc gagttagtcc
    27841 cgactggtct ggcattactt ttcctggtgg tcatgttgaa cgtggcgaat cctttgtcga
    27901 tgctgtcatt cgtgaagtga aagaagaaac tggtctgatc atttccaaac cccaactctg
    27961 tggtatcaaa aactggtatg acgacaagga ttatcgttat gtcgtccttt tttacaagac
    28021 agaacacttt actggtgaac tccagtcttc agacgaaggg aaagtttggt gggaggactt
    28081 tgaaaatctt tctcatctaa aacttgcaac cgatgatatg tctgatatgc ttcgtgtgtt
    28141 tctagaagaa gatctcagtg aattctttta ctacaaaaac ggtgacgact ggctttatga
    28201 tttgaagtaa aaaataggct aggaaattcc tagcctattt tttctgcatt tgtctgaacg
    28261 taagccgcaa tggcatctga cgtgtactgg gctgtttcag aaccaaactt gtcaatgttt
    28321 tccttaaact ctgggttgta gacgtaacct ttaccgatat gaccgaatac ctcaatagag
    28381 cagtcaaatc cataagtgcg aatggcttgc aagagcttgg ctgcttgctc ctggttttcg
    28441 gttgctgttg caggtaaacc aacttgaaga ttttgtgcca aagtttgaaa gacttggtta
    28501 aaggcggccg tagcctcgtc ttcgtgacct ttttggcgtt cgagcgcttg tcccatgact
    28561 tcttgaccat atttctctac cgcttcttgg tggtattttt gattgtcttg atagctaaat
    28621 cccgtgaatt tttcctcaat ggtcattttt ctttctcctt tttgttcttg aatagttttt
    28681 tgcaaggtgg aaatcaaggt atccagatgt tgcctttcgc gagttagata gtccaactac
    28741 ctagtcaaat ggagcaataa atctgtcctt tcttccttta acagttctgc tattttctct
    28801 aaagaaaagc ctagatattt gtagtaaaga atgacctgaa ggcgttccaa atcctcttga
    28861 ctataggttc gatagccgtt ttccgacttt aaggggacca agagtcctat cttgtcatag
    28921 tgatgcaggg cggggtcttg acagagacac ctgaaagctg cgcagcttct tttatatggt
    28981 acatgatttc ctccttacaa tgatagtata acccctcccc ttaggtcaga gtcaagcgtt
    29041 tttgagaaag gtgtgaaaac ttgaaaatgg ttttcttgac agaccttaga aaacataata
    29101 ggatagtcaa gtctatcaat caaacagaag gctatttgac aactcaagta gctttttctt
    29161 attttgaaaa aggagatcag agtttaacta tgtcagaaaa atcacaatgg gggtcgaaac
    29221 ttggctttat tctagcatct gctggctcgg tcatcgggct tggttccgtt tggaagtttc
    29281 cctacatgac tgctgctaat ggcggtggag gctttttact aatctttctc atttccacta
    29341 ttttaatcgg tttccctctc ctgctggctg agtttgccct tggccgtagt gctggcgttt
    29401 ccgctatcaa aacctttgga aaactgggca agaataacaa gtacaacttt atcggttgga
    29461 ttggcgcctt tgccctcttt atcctcttat ctttttacag tgttatcgga ggatggattc
    29521 tagtctatct aggtattgag tttgggaaat tgttccaact tggtggaacg ggtgattatg
    29581 ctcagttatt tacttcaatc atttcaaatc cagccattgc cctaggagct caagcagcct
    29641 ttatcttatt aaatatcttt attgtatcac gtggggttca aaaagggatt gaaagagctt
    29701 cgaaagtcat gatgcccctg ctctttatca tctttgttgt tatcatcggt cgctctctca
    29761 gtttgccaaa tgccatggaa ggggttcttt acttcctcaa accagacttt tcaaaactga
    29821 ctagcgctgg tctcctctat gctctgggac aatctttctt tgccctctca ctaggggtta
    29881 cagtcatgtt gacctatgct tcttacttgg acaagaaaac caatctagtc cagtcaggaa
    29941 tctccatcgt agccatgaat atctcgatat ccatcatggc aggtctagcc attttccaag
    30001 ctcggtcccc cttcaatatc cagtctgaag ggggaccgag cttgctcttt atcgtcttgc
    30061 ctcaactctt tgacaagatg ccttttggaa ccattttcta cgtcctcttc ctctttgcga
    30121 ctgtcacttc ttctgtcgtg atgctggaga tcaatgtggg caatatcacc aaccaggata
    30181 acagcaaacg tgccaaatgg agtgttattt taggaatttt gacctttgtc tttggcattc
    30241 cttcagccct atcttacggt gtcatggcgg atgttcacat ttttggtaag accttctttg
    30301 acgctatgaa cttcttggtt cccaatctcc tcatgccatt tggagctctc tacctttcac
    30361 tttttacagg ctatatcttt aaaaaggctc ttgcaatgga ggaactccat ctcgatgaaa
    30421 gagcatggaa acaaggactg ttccaagtct ggctcttcct tcttcgtttc ttcgttccgt
    30481 cattccaatc atcatcgtgg tcttcatcgc ccaatttatg taatcaaaaa ggacttgagt
    30541 aatgaactca ggccctttct tttttatgga tgactaacaa ttaattctaa atcttgccct
    30601 tccagagtcc aagcttcaac atcacttggt aggataaagt gtctcccttt ttggattgga
    30661 tagtttttcc cgtcaacagt tagttgacct tgaccagcca agacactcaa caagctgtag
    30721 tcagctgtct tttcaaagtt aacttttcca gtaatttccc atttgtaaac tgcgaagaaa
    30781 tcattggata caaggagagt ggaacgtaga tcatctgctt tgattgtcac tggacggcta
    30841 tttgcgggtt caccaatgtt caagacatcg atagattttt caaggtgaag ttcacgtaaa
    30901 ttgcccttgt catccttacg gtcaaagtca tagacacggt aagtggtatc gctagactgc
    30961 tgggtttcaa gaatcaagat acccgccccg atagcgtgca tggtaccgct tggtacatag
    31021 aagaaatctc ctgctttaac tggcactttt gtcaacagcg catcccagtt cttatcctcg
    31081 atttgctggc ggagttcttc ttttgacttg gcattgtggc cgtagataat ctctgaacct
    31141 tcatccgcag cgatgatgta ccagcattct gtttttccaa gttcgccttc atgctctagt
    31201 ccataagcat cgtctgggtg aacttgaacg ctgagccagt cgttagcatc caaaatctta
    31261 gtcaaaagcg gaaatacagg ctcaggacga ttgccaaata attcacggtg ttccgcatac
    31321 aaagtagcaa gatctgttcc ctcgtaacga ccattggcaa ctttagaaac accatttaga
    31381 tgagctgaga tagcccagta ctctccgact ttttcacttg ggatgtcgta gccaaactca
    31441 tcacgtagct tggtaccacc ccagattttt tcttgcataa ctgattgtaa aaataatggt
    31501 tctgacatct taatctcctg tctgattttt ctcccctcat tatagcaaaa aaagagttcg
    31561 aattgaactc ttttttacat cttataaagc agggagaaga ttttataaaa atagtaaaca
    31621 aatgtgctct actcgatgct tacaccattg ctataaatga catgtaccaa tagaaggact
    31681 tcttcttgct acgtttgaga gtcccatttc ctacattatc tcgatctaca tagataaagc
    31741 catagcgctt attcatttcc cctgtgccag ctgaaaccga atcgatacag ccccatgtcg
    31801 tataaccaag caagtcaacc ccgtctttgt aaatggcatc tcgcatggcc ttgatgtggg
    31861 cctctaagta agtaatccga tagtcatctg ctacataacc attctcatcc ggtgtatcca
    31921 tagcaccgag tccattttct acgataatac tatactaaaa tcaaaaagca ttatataata
    31981 gtgatatgaa atcaactaaa gaagaaatcc aaaccatcaa aacacttttt aaagactctc
    32041 gtacagctaa atatcataaa cgccttcaaa tcgttctata gtaaaacgaa acaagaacag
    32101 ggcaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac
    32161 tattctagtt tcaatttatt atatttcgtc tgatgggcaa atcttataaa gagattatag
    32221 aacttttata gtagtttgaa ataagatgtg aacaactcta tcaggaaagt caaactaatt
    32281 tatagaaata ttttagcagc taaggtgtac tgttatagat tcaatacact atagactgta
    32341 atcaaacaac gatttggcga aatgtaaaaa aatatgagga gttcgaactc gactctctcc
    32401 ttcaagaaac acgtggtggc cgtaaccatg catatatgac gattgaggaa aagaaagtct
    32461 ttcttgcccg ccatttgaag gctacagagg caggagaatt tgttacaatt gatgccttat
    32521 ttcaggctta taaaaaggag ttaggtcgtt cctacacacg tgatgccttc tatcaactgt
    32581 tgaagcacca tggttggcga aatattatgc cacgtccaga acatcctagg aaagcagacg
    32641 ctcaaaccat tgtcgcgtct aaaaataaaa tctcaattca agaagaaaag aaagcgcttt
    32701 aaaagcagta gacgttttcg taaggttcgc ttgatgtacc aagatgaggc tggtttcggt
    32761 agaatcagta aactgggatc ttgttgggct ccaataggag taggtccaca tgtccatagt
    32821 cactatatac gagaatttca ctattgttat ggagctgttg atgcccatac aggcgaatca
    32881 tttttcttaa tagctggtgg atgtaatact gagtggatga acgccttttt agaagagctt
    32941 tcacaagctt atccagatga ttatctttta ctcgttatgg acaatgctat atggcataaa
    33001 tcaagtacct taaagattcc gactaatatt ggttttacct ttattcctcc atacacacca
    33061 gagatgaacc cattgaacaa gtgtggaaag agattcgtaa acgtggattt aagaataagg
    33121 cctttcgaac tttggaagat gtcatgaatc aacttcaaga tgtcatacaa ggactggaga
    33181 aggaggtgat aaagtccatc gttaatcgga gatggactag aatgcttttt gaaagcatat
    33241 gagtattata tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt
    33301 catattctca atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct
    33361 atttcatacg ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg
    33421 ttccccaatc ggttacagtt ggtccgtgta aaacctttat accaagctcg ttcaaccgtt
    33481 tgtagttctg gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt
    33541 ccaaaggaac caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag
    33601 caaaaccatc atcagcataa tctgcctttt tatccaaaat atgctccaag tcagcacaga
    33661 cttggggaac atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat
    33721 aaaaagaccg gattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag
    33781 tctacaagtt tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa
    33841 tccttcttct tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt
    33901 gaaggctttg atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg
    33961 aagtggacgg tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa
    34021 tttaacagct tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac
    34081 agctccttga gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa
    34141 gtgtttgttt gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac
    34201 aaggttgata actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt
    34261 gaatgctccg tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc
    34321 gataccacca ggaagggcaa tacccattgt cgcaaagagt ggagatgtac gccacatgtt
    34381 cttaggtgtc atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga
    34441 gtagatagcg tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa
    34501 ttcaccctca gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt
    34561 aacgtttgca cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt
    34621 agctgcttga ccagcatcac caaggattga agcgtcaagg gcatgacgtt taccaagttt
    34681 gtaagggtcg atatcaactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc
    34741 agcaaatggg aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt
    34801 ggctggtttc caaccaacac gataagcaga acctgtcaaa ccttcatagt tccattcgaa
    34861 agcttcaaag tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt
    34921 aatcacttca ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt
    34981 gttcaagatt tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga
    35041 gcgttcgtat gaacctgaac cgtagtatga gttttcgtcg atttcttgga aaccgaagtt
    35101 tactggaatt tcaacaacag ctggaccttt tttagaaact gcagcacggc aggcttcgtc
    35161 aattactttt ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt
    35221 gtacattggg ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg
    35281 tgatccaagg attgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat
    35341 caagtgagtc gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt
    35401 agcttgcata accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaagcggat
    35461 atctttgtct tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta
    35521 gattgtatct acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt
    35581 cccttgagtc ataatgataa ctctccttca atttttttag acttggagaa tacgattaca
    35641 tagaattgga aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt
    35701 ttctaaaaat ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagtgtcgt
    35761 catttctgtt ttataacaaa aaaatctagt cattactttt agtctatttt actaaaattt
    35821 aacagaaggg aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat
    35881 tctgggttga gagccagtcc aacacctgaa aagactcctg ctgcaatcgg aattccgaca
    35941 acattgtaga taaaagccca gaaaagattg agcagaatac gatgaaaggt tttcttactc
    36001 atgtcgaagg cacgcaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac
    36061 tcgatggcga tatctgttcc agctcccata gcaatcccca catctgctac actaagggca
    36121 ggagcgtcat tgatcccatc accaacaaag gctactttac ctgactgttg cagtttatgg
    36181 atttcatggg ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga
    36241 tctgcaatag cacgcgccac accagcattg tctcctgtca gcatgactgt cccgagacca
    36301 cgttttttta gctgactgat ggctagctta gcatttttct taggaatatc ttgcaaagca
    36361 agcaagcctt tgatttcatt gtcaacagct aagaacacaa ctgtcttagc ttctttttct
    36421 agttcttcta gtttatcttg ataagtatta gaaatatcca tgccatccag cattttagca
    36481 tttccaagta aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggcc
    36541 tgaaaatttt caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc
    36601 tcagccagtg ggtgttgaga agcatcttcc aaggaggctg ccaatccaaa cacttctact
    36661 tcgtcgccga tgacatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca
    36721 aagacaaggg tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc
    36781 cccatcttgg cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag
    36841 gcacaaggac aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta
    36901 gctccaagca caaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt
    36961 cctaaaatga caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc
    37021 ggcgcacgac ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct
    37081 gagccaactt tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg
    37141 acagtatctc caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca
    37201 atactagaga caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact
    37261 cgaatcaggt cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc
    37321 aagacttctg cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt
    37381 tttctcattt tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca
    37441 ctttcgaagt aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact
    37501 agagttccca gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa
    37561 gcactctgga tatatggctt acctgcaact aacataatag gcgttgttgc tagaaaggtt
    37621 ccccaatgca tgacttgatg actaatgcta cctgccaaca tcccaatcat gagaatcaca
    37681 agaggcacag tacagatact agtaatccaa aaacgttgca ggagagatag agattttcga
    37741 gtcttctcaa cgactgtata gcttcccttt tgcatcttca tgccacaaga aaattcatgt
    37801 cgccctaatt cttgaggtgt aaaacgaatg actttctcct catctacgcc gattggttcc
    37861 aagatacctt cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga
    37921 aaggtaatct cagctggaat tcccttttga agctggatat gggctggatg atagcctttt
    37981 tcagcttgga tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc
    38041 atagtctcca cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag
    38101 ctaaactctc cagcctgttc aggcgtgatt tccactacat actcttcccc cattggcagg
    38161 ttcgcatgta caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatccttg
    38221 cggtcaaaga caatgcgggc tggcactgat ttcttgagga caatcaactc aggagtatag
    38281 cctcccatga cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca
    38341 gatttttcag gctttttgaa aaaccaaaac aagataaacg cgataagggc aatacaaata
    38401 atggttacaa tactatttaa catgacgtct cctttacata caattacatc ttacttctgt
    38461 tacagcactt gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt
    38521 aaattcacat tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt
    38581 gtctttgata tcttggacaa gtaaatcccg actttggtct agagttaaaa gcgctgaata
    38641 aacaaaggac ttgccttctt ttttccgagt caaacactct ttctcaacta gacgagccaa
    38701 aagtgtctga accgtggact tggaccagtc aaaccgctct gccaaaaccc taatcaaatc
    38761 tgtactggtc tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga
    38821 aatctgcatt accatacctc caaaatctac atttgtcaat tacactcaac agtatactcc
    38881 caaaatctac atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc
    38941 atttgaaaaa cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta
    39001 aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc
    39061 ctctgactca atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca
    39121 gtcttctatt gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg
    39181 ttgggaggtt ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta
    39241 gtggtggtgc tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa
    39301 tggctgttgt aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg
    39361 tttctcctca aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg
    39421 ctgtaaaaac tggaatgttg gctactactg aaatcatgga aatcatccaa ccctatctta
    39481 aaaaactgga ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct
    39541 tgattgactc aagtgctaga gactatctca aaacaaactt actacctcta gcaactatta
    39601 ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag
    39661 acatgcagcg tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca
    39721 aaggcggaca tctcaaaggt ggtgctaaag atttcctctt taccaagaat gaacaatttg
    39781 tctgggaaag cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg
    39841 cagtgattac tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca
    39901 aggcctttat cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag
    39961 tcaaccatac aacttttaaa gattaagaaa aaaaactctc tagttcccac tttaagggaa
    40021 ttagagagtt tttatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcaac
    40081 ttcaaaacac tgttttgagc tgacttcgtc agtcttatct aaaacctcaa ggcagtactt
    40141 tgagcaacct gcgactagct ttctagttta ctctttgatt ttcattgagt attaattagg
    40201 aaagaatgtc atgcaacttt gttaaaaagg cttgcgtttt tgcctcaata tcttctgctt
    40261 gcatcaaatc acgtacaaca gctacaccag ctatgccagt gcccataagc tgatcaatat
    40321 tctccgaagt caagccacca atagcaacta ttggaatggc aaccgtttgg caaattgttt
    40381 tcaaggttga aatcagggtg atgggtgcat tttccttggt ggttgtcgga aaaatggctc
    40441 ctgtccccaa gtaatctgca cctgatttct ccgcttccag agctctttta accgttttag
    40501 cggtgacacc gaggattttt tcaggaccca agacttgtcg ggcaaccgaa actgggagtt
    40561 cgtcatctcc aatatgcaga ccagcagcat caaccgcaag acaaacatcc aaccgatcat
    40621 cgattatcaa gggtacctga taagcatctg ttatttcctt gacttgtttt gccagttgat
    40681 aatattgatt ggttgtgaga tttttttctc gcaattggac tatggtaacc cctgaacggc
    40741 aggccgtctc aacttttgca agaaagcttt ccacggaatc ttgatagcga ttggttacca
    40801 gatatagtct aagtgcttct ctattcataa acctctcctt tgatggtatc tagccaattt
    40861 tcatctcttc ttaggagcga aagctgattg agtacttggt aacgaaattc ttccaatccc
    40921 attccttgaa caactatttt ctcagcagcg atattgagat aagagactgc taagcaagaa
    40981 gcttcaaaac cagtctttcc ttggctgaga aaaacggctg ttaaggctcc aaccaaatct
    41041 cctgtccctg ttatccagtc taattctgca cagccatttc ccagtacagc gacctgattt
    41101 ttcgaaacga cgaggtcctt gggacctgtg actaagaaag acataccatg ataggtctga
    41161 caccagtctt tcaagacttg aagcaaatcc tccgtttctt gatctttagc actcgcatcg
    41221 accccaacgc cgtggtgctt taatccaaca agacttcgaa tttctgacat gtttccttta
    41281 aggaccgtag gtctatagtc taaaaggtct ctgactaagc tcttacgaat ggatgaagct
    41341 gttacgccaa ccgcatctac taccattggg agacaaactt gagctgcata agaagctgct
    41401 atacggattg ctttctcctt ctcagctgac aaatgcccca aattgatgaa gagagcctga
    41461 ctttgcttag taaaatcaag aacctcacgg ggatcatctg ccatgacagg tttgcatccc
    41521 agagccaaaa tcccatttgc cagcatctca caagaaatct cattggtcat acagtgaatg
    41581 agggaactag agcctatagg aaagggattt gtaaattcct gcatcagtct atcctttcac
    41641 taaagaaata tccctgcact tttttaaaga attcctgctt gattaaaaat cgaaaggcaa
    41701 taaaggaaat cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc
    41761 agctaagctt agtagctgat cctgtaaaga gtaccataac aggataggaa acaatagaac
    41821 caataatacc tgttcccaca atttctccca aggcagaaaa gtaaaatttt cgaccgtact
    41881 tataaaagag acctgctaga agggctccaa aagtcgctcc tgtgagagct aaaggcggaa
    41941 tcccttgagt cgtcatacgg ataaaggctg tcactgtagc catagccaag gcataaacag
    42001 gtcccatcat gattcccgct agaatattga ctacactgga catcggtgcc attccctcaa
    42061 tccgaaagat aggtgtaagg actacatcaa gggcaatcat catagataaa atggtcaatt
    42121 tgtgaacttg tagttggtgc tttctcatgt ttctattctt ctcctttttc taaagactgt
    42181 aaatcgctct tccatgtctg gtgttggtaa gccatctccc aaaacttggc ttccatatga
    42241 acactgatgt ggaaggcatc tagcattttt tgcttatctg tctcatcact ttctcgatag
    42301 agctgattga ccagtgctcc ctcctctctg atctgttgct ctaactcatc cgtaatataa
    42361 gtttcaatcc attgttgata gagaggattt ggtgatggtt taagattaag tgatttgcct
    42421 atatcatggt ataaccaagg acaaggaagc aagcttgcaa aagcgatggc taagttcggt
    42481 tctgcaaatt gcctataaat atgagaaatg taatgataac aggttggagc gattggatgt
    42541 tgctccattt cctggtcgct gatttccaat tccttgaaaa attgttggcg aataaataac
    42601 tcaccctcca ctaaaccctg agcattttgt ttcaagagtc ttttcatctc ttggtttgaa
    42661 gtcttatcag ccaaaagatg atagatttct gagaaagcct tcagatagta ggcatcctga
    42721 atcaggtaat agcggaaaat ggcaggttct aaattcccct cttgtaattg taaaataaag
    42781 ggatgatgaa aggaagcctg ccaagctttc ttggataatt ccatcgcaat atctgtaaat
    42841 tccataataa ctcctttata aaaatagact ggtttgaagc aataaaaaga aaagcaggta
    42901 gattaatttt gtttttttag gaatataaaa agtccgatag ctattcttca actgtgcatg
    42961 ttcgtcatat ccgtgagcag atagagctct caggtaaaga tggcgccacc taaagactgt
    43021 catcagaacc ttactgtaaa tcaagggcga ccaaaaatgt agttcttgac cacgtaatag
    43081 gcaagcttct ttgagggact tgatttcttg ctgaatgaga ggaaaggaat tgaataccac
    43141 aatcaaggca taggaccaag agcgtgatag ccccttttga gccaagtaca agagaagctc
    43201 ttttagtgaa atagaggaaa caaagacaag gccgatacaa actgtcacaa aggccctcgt
    43261 tccaagcatg actgcctgtg aagcatctcc gtgtaactga actgcccagt agttggcaaa
    43321 agatggtaaa atggcaagta tgatcatcca agctaacatt ttaaatcgac ggtaatagag
    43381 cataaagaga atacaaaatg cgactaccga aagattcaga gcaatcgaag gaatgaaaga
    43441 tgtttccaag gataaaatca gcaagaagag actgataatc ggtgtctggg ttgctacttt
    43501 gaccatacta tctcacctcc ccttgggtat tgctactctg agatgtaagt ggtttggtaa
    43561 tggtcacttc tttcacatgc cgaagaccct gactagtcat ctcaatccaa taatcaacca
    43621 cagaaatcaa agggtctaaa cgatgactaa tgagcagaaa acttcttcct tgattcctct
    43681 cctccacaat ccacttgcaa aaataatggc aggctctatc atccaaacct gcaaaaggtt
    43741 catctagcaa gatcacggaa gccttactgg tcaagatggt caagagctga agaatttttt
    43801 gctgaccacc acttaattga tagggactct tatcgactgc ctgctccaaa tcaaaatatc
    43861 gtaaagcttg aaaaatccgc tgatttcttt cagaatcagg tccatctaat tgaagctcct
    43921 ctcgcagact gactcggata aactgcttct cagcttcctg aacaataccc gtcagatcac
    43981 ggtacaaact ctttttcttt ttcaggactg tccccttcca tgcaatgcgc cccttatact
    44041 tttgaaattg aagaatagac cgaaagaggg ttgatttccc gacaccattg tcacccagga
    44101 tacaggaaat cccttgatag aatgtgaaat cagcaattga aaagaggggg cgattaccaa
    44161 gctcaccagt cacacggttc atatggaata attccgggct agaagcaact tcctttgaag
    44221 caacctgtgt catctcatag gaagggattt gaaacacttc ccttagtttt ccgtctctta
    44281 gctcaaccat atggtcgata taggctttat agtcagataa atcatggtcg cacaaaataa
    44341 ctgtcttccc atcaagagcc aactctttta gaatctccaa tatctcgatt ctgctcttgc
    44401 ggtcaatgga agcgaagggc tcatccaaga gatagaccct aggattcatg gcaaagagga
    44461 cagccagcgc tgctttttgc ttttccccac ctgataggtg atgaatggga cggtgtaaga
    44521 ttgcctcgca acgacattgc tggacaacct ctgctatttt agaatcaatt tcctgaaggt
    44581 gatagccgat attctccaag gtgaaaatca gctcctcaaa caagttctcc atggtaaatt
    44641 gatgattggg attttgaaag agaatgccaa ccttctggac acgttcgacg atagaaagct
    44701 gactgacctc actcccatct atcaggactt gaccgctata gggaagagaa ctgacttggg
    44761 caatcatttg aaagaggctg gattttcctg aaccactgct cccaactaac aaggtaaagg
    44821 cttgcgcatg aaaagtaaaa tcaaacggct cagagaaaat tggggactga atcgctcgta
    44881 gttccagacc catctatgct tttcctccag ttgcaaactg atgatagagt ttgacaatgg
    44941 cacgaaccaa gatggtacag aagaaataaa cagaaataaa acgtaccaca agcaaggaaa
    45001 ggacaaacgg aagggaaaag gcgtagtaac ctaacttaat gtattcatag acaaagctaa
    45061 caagcgtaat cccaatacta ttagcagtta gagagagcca actttcatag cgattcttag
    45121 ttacgataaa accaaattca cttcccaaac cttgaaccaa gccagacaaa agagctccta
    45181 gaccaaattg gctaccataa aggacttcag caagcgcagc tagcacttct ccaatcgttg
    45241 cacttccgac tctcggaaca aagatggcag caatgggcgc agccatacac cagagaccga
    45301 agaggatttc attggcaaag gcctgcaaac caagaggtgt taagagtaga ctgagaatat
    45361 tatacacata tcctgaacca acgaaaaccc caccaaaaaa gatagacaag aaagcaagca
    45421 agataacatc ttttaactgc cattttttca acataaaaaa actccttttt ttaaagaaaa
    45481 gtggggcact caagaagacc gacctaaata ctttgtatag cagactgaat ttagaacagt
    45541 acacaagaac actaaaatat ttctagaaat taatttgaat tttctaattg atttgttcgc
    45601 atcttatttc aatctactat atcatcttca tccagtttcg taaaagaaaa aactctaatt
    45661 acagatacga attagagttc agcttacaag attagacagt tcttttcgac atacgaaaaa
    45721 aacatttcac atttcccttc gccagtctta actgtatcag gttcaatggg tatcatctca
    45781 gcctaaagca ccccaaatgt ctttattatt taattatgtg attattataa cacacatttt
    45841 atactagttc aagaaattga actggaaata cagccttgca ctcacaaaga cagcagatct
    45901 ttcttttgca aaaaacaaat gacctgtttg atgaattagc cattcaagct gaatctggac
    45961 atagcttttt aaaaaaggaa aatcctactt acttagaatc caaggataga tatctattgt
//
LOCUS       DAVMMR010000009        43852 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMMR010000009 DAVMMR010000000
VERSION     DAVMMR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.009"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(1..297)
                     /locus_tag="VYS74_000487"
     CDS             complement(1..297)
                     /locus_tag="VYS74_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788569.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VYS74_000488"
     CDS             complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VYS74_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV3788570.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3570..4796)
                     /locus_tag="VYS74_000489"
     CDS             complement(3570..4796)
                     /locus_tag="VYS74_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV3788571.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4839..5531)
                     /locus_tag="VYS74_000490"
     CDS             complement(4839..5531)
                     /locus_tag="VYS74_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3788572.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5704..7455)
                     /locus_tag="VYS74_000491"
     CDS             complement(5704..7455)
                     /locus_tag="VYS74_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788573.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7445..9190)
                     /locus_tag="VYS74_000492"
     CDS             complement(7445..9190)
                     /locus_tag="VYS74_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788574.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9396..9692)
                     /locus_tag="VYS74_000493"
     CDS             complement(9396..9692)
                     /locus_tag="VYS74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV3788575.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9711..9848)
                     /locus_tag="VYS74_000494"
     CDS             complement(9711..9848)
                     /locus_tag="VYS74_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788576.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNIA
                     P"
     gene            complement(9845..10300)
                     /locus_tag="VYS74_000495"
                     /pseudo
     CDS             complement(9845..10300)
                     /locus_tag="VYS74_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10434..11261
                     /gene="xth"
                     /locus_tag="VYS74_000496"
     CDS             10434..11261
                     /gene="xth"
                     /locus_tag="VYS74_000496"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV3788577.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAKLDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11273..11416
                     /locus_tag="VYS74_000497"
     CDS             11273..11416
                     /locus_tag="VYS74_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3788578.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11527..11655
                     /locus_tag="VYS74_000498"
     CDS             11527..11655
                     /locus_tag="VYS74_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV3788579.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     regulatory      12038..12133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12533..13114
                     /locus_tag="VYS74_000499"
     CDS             12533..13114
                     /locus_tag="VYS74_000499"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV3788580.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13114..14376
                     /locus_tag="VYS74_000500"
     CDS             13114..14376
                     /locus_tag="VYS74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV3788581.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14427..14888)
                     /locus_tag="VYS74_000501"
     CDS             complement(14427..14888)
                     /locus_tag="VYS74_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV3788582.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14885..15649)
                     /locus_tag="VYS74_000502"
     CDS             complement(14885..15649)
                     /locus_tag="VYS74_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV3788583.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15830..16234)
                     /locus_tag="VYS74_000503"
     CDS             complement(15830..16234)
                     /locus_tag="VYS74_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV3788584.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(16304..17785)
                     /locus_tag="VYS74_000504"
     CDS             complement(16304..17785)
                     /locus_tag="VYS74_000504"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV3788585.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            complement(17804..18982)
                     /locus_tag="VYS74_000505"
     CDS             complement(17804..18982)
                     /locus_tag="VYS74_000505"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV3788586.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19146..20153
                     /gene="galR"
                     /locus_tag="VYS74_000506"
     CDS             19146..20153
                     /gene="galR"
                     /locus_tag="VYS74_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV3788587.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20199..21191)
                     /locus_tag="VYS74_000507"
     CDS             complement(20199..21191)
                     /locus_tag="VYS74_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3788588.1"
                     /translation="MDLRPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGH
                     EAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQA
                     EYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVI
                     GDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREIL
                     GGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAA
                     SATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE"
     gene            complement(21226..21579)
                     /locus_tag="VYS74_000508"
     CDS             complement(21226..21579)
                     /locus_tag="VYS74_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV3788589.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21823..22713)
                     /locus_tag="VYS74_000509"
     CDS             complement(21823..22713)
                     /locus_tag="VYS74_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV3788590.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGAVVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSDLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22846..23382
                     /locus_tag="VYS74_000510"
     CDS             22846..23382
                     /locus_tag="VYS74_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3788591.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(23549..24355)
                     /locus_tag="VYS74_000511"
     CDS             complement(23549..24355)
                     /locus_tag="VYS74_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV3788592.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24700..26220)
                     /locus_tag="VYS74_000512"
     CDS             complement(24700..26220)
                     /locus_tag="VYS74_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV3788593.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVSKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26213..26941)
                     /locus_tag="VYS74_000513"
     CDS             complement(26213..26941)
                     /locus_tag="VYS74_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788594.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(26956..27720)
                     /locus_tag="VYS74_000514"
     CDS             complement(26956..27720)
                     /locus_tag="VYS74_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV3788595.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(27724..28167)
                     /locus_tag="VYS74_000515"
     CDS             complement(27724..28167)
                     /locus_tag="VYS74_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3788596.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(28849..29145)
                     /locus_tag="VYS74_000516"
     CDS             complement(28849..29145)
                     /locus_tag="VYS74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3788597.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(29167..30231)
                     /gene="pepA"
                     /locus_tag="VYS74_000517"
     CDS             complement(29167..30231)
                     /gene="pepA"
                     /locus_tag="VYS74_000517"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV3788598.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30632..31333
                     /gene="galE"
                     /locus_tag="VYS74_000518"
     CDS             30632..31333
                     /gene="galE"
                     /locus_tag="VYS74_000518"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3788599.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            31756..32715
                     /locus_tag="VYS74_000519"
     CDS             31756..32715
                     /locus_tag="VYS74_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788600.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            32705..33661
                     /locus_tag="VYS74_000520"
     CDS             32705..33661
                     /locus_tag="VYS74_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV3788601.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLIAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATSLVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            33658..34410
                     /locus_tag="VYS74_000521"
     CDS             33658..34410
                     /locus_tag="VYS74_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3788602.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            34506..35471
                     /locus_tag="VYS74_000522"
     CDS             34506..35471
                     /locus_tag="VYS74_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3788603.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(35696..35938)
                     /gene="yidD"
                     /locus_tag="VYS74_000523"
     CDS             complement(35696..35938)
                     /gene="yidD"
                     /locus_tag="VYS74_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV3788604.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(35938..36660)
                     /locus_tag="VYS74_000524"
     CDS             complement(35938..36660)
                     /locus_tag="VYS74_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3788605.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(36647..37216)
                     /locus_tag="VYS74_000525"
     CDS             complement(36647..37216)
                     /locus_tag="VYS74_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV3788606.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(37235..37963)
                     /locus_tag="VYS74_000526"
     CDS             complement(37235..37963)
                     /locus_tag="VYS74_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV3788607.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(37963..38703)
                     /gene="xerD"
                     /locus_tag="VYS74_000527"
     CDS             complement(37963..38703)
                     /gene="xerD"
                     /locus_tag="VYS74_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV3788608.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(38694..39155)
                     /locus_tag="VYS74_000528"
     CDS             complement(38694..39155)
                     /locus_tag="VYS74_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3788609.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(39152..39673)
                     /locus_tag="VYS74_000529"
     CDS             complement(39152..39673)
                     /locus_tag="VYS74_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV3788610.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(39649..40620)
                     /locus_tag="VYS74_000530"
     CDS             complement(39649..40620)
                     /locus_tag="VYS74_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV3788611.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(40617..41411)
                     /gene="racE"
                     /locus_tag="VYS74_000531"
     CDS             complement(40617..41411)
                     /gene="racE"
                     /locus_tag="VYS74_000531"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV3788612.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(41618..41866)
                     /locus_tag="VYS74_000532"
     CDS             complement(41618..41866)
                     /locus_tag="VYS74_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV3788613.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(42122..43747)
                     /gene="treC"
                     /locus_tag="VYS74_000533"
     CDS             complement(42122..43747)
                     /gene="treC"
                     /locus_tag="VYS74_000533"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV3788614.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
BASE COUNT        12736 a        10014 c         8168 g        12934 t
ORIGIN      
        1 ttatttcatt ttattatact ctgttaattc atatgagttt aaaccgattt catctttaac
       61 ctcgagtaaa gcagtttcaa atatttgttt aagagttttt gattctttac aattaaccga
      121 caaactttct gataaaatat gtacaacttc tgagactgaa taacctatct cctctttaga
      181 attatataaa tctgtagctc caccaataat ccaaaaatac tgattttgtg aacctacaat
      241 atcctcattt tctacggaac ttccttgtat cgaacaaatt ttatttatct ttaccataat
      301 acttcaaccc ttttagtgtc aaaagtaaac caattcctgt cactgttaag aatagttcca
      361 taatcttgtt cgaaccagtc tttggtaatt tttgttttac atctactatt tctttagatt
      421 tattaatatg attttcagtt tctctgccat ctccaactat tttatagttt acttcttctg
      481 tcttattatc ttgtttattg tcgatcttgt cattcatttg tctattatct ttacttgagt
      541 taaactctcc gttcttctgg ttactatcaa ttacattatt tgaattagat tgtttttcct
      601 ctttgttttt ttctttttcg tttttatcac ttaaattatt tgttacaatt ttgtaaagcc
      661 cattctccgt tacaatattg aaattaccat cgctatcacg tataacaggt tctttcccat
      721 ttgcattaga tttgatgaat gatatatact taccggataa attataaaat tggttattta
      781 aaacggttat tttacccttt gaatcctcaa taacaattcc ttctttacca ccgaatatac
      841 tattctctga cactactaaa ttatcactaa ctcgtaataa ttcaatattt ggttgttctg
      901 tttccttaga attattatta attacatttt ttgttacagt aatatctgat acttttccta
      961 aatattctgc actatctttt gcaactcgta tcgcttttgt tttaggatcg gcaatattaa
     1021 atatattttc ggcgataacc acctgtttat ttaaatctaa taggtcttta gtgtttttat
     1081 agctataagc atttactaaa gctgctccgc tttctcgata atgtacactc tctcctttaa
     1141 ctttcttatc aaagcgattt cctttgatta atacatcagt gaatcctgta aaacgtacac
     1201 ctgcatacat catgttatca aaatgattat ttaaaatttt aatattagag gggttctgtg
     1261 tcgacaatgt ttgatagtgt gtgccaattg ctgttactaa ttccccagat ttatcacttt
     1321 tgccaaaata ggaattttga atagtcacat tttcagattt tttcccatca tcattcaagg
     1381 cataaggaaa accttttcta gttaatggtt caatctgaat gctctcctta cttatgattt
     1441 gcccatcctt catcgttttg ggtaaggctt gcccaagaaa acgagaatta tcaactaata
     1501 catttttcga acctgcaatt tgaatagcat gcccttgata actatccttg aatgttacat
     1561 tttttatagt tacgttattt gaattcccaa tagcaaatgc acctgaagaa tttataagtg
     1621 gtagattttt tgctttagtt ccttcttcat tcaaagcacc gttcatatca atcgtaccac
     1681 cagaataact aatatcttct gttgggcccc attctacttg cgcaccatca tccgtaaata
     1741 aacctgtcat aaaaacaatg gaagggtgat tcttaatatt tataccattt agaattgtag
     1801 ctttctcatt caattctaag tgtgtatgac tttttaaaaa aacaatttct tttactaaat
     1861 aagttccttc aggaaaatat acatttcctc cacctagccc ttgagctgca gcatctattg
     1921 catcttgaat tgcttgtcga tcatcatgaa tcccatcacc tactgcacca taatctttta
     1981 cacttaaagc agtatcttca aattcttttt tgataattct gtcacttata acatcttcct
     2041 tataactctt attttcatta gtagatgtta ctgtagattc aactgttctg ttgtctaatt
     2101 gtgaattgga acgaatagta ccattagagc tgatactatt aacacttaac gaattatcta
     2161 aaccattagt ggttgaatta gaggcaatca tttcagcaga acgattctga ttaacagttg
     2221 gtgaattatc taaatcatta gtaattgcat tagaagactc ttttgtagcc atcggtgaag
     2281 aactagtaac aacttcatcc gataaaatac cttggcttcc caaaattgaa attgccaata
     2341 gtaccgagca agtccccacc tttaatttac gaatactgaa tacctcatta ggaacaaaat
     2401 atttcatcag aacctccaat ttttttacta tttttaacaa ttgattatta cttttcttaa
     2461 aatagataga atgacaccgc tgtcattatc tttatcatat attttccaaa ttgaattgtc
     2521 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
     2581 cagtgccgcc ttgccctaag tctaggttac tgacttggtc agttctatag tagactgaaa
     2641 ctataatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcccgatc
     2701 gatttgtcct attcttattt cattttatta tatctacaac ctcaaagcag ggttttgagc
     2761 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
     2821 tcttagcttg aatataaaat atcccattta ctatatttaa atttgttgat atgttgtaaa
     2881 ttataaatga aagaacaaat atcttgtcat acaagcattc gctagggata tttacctgaa
     2941 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
     3001 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
     3061 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcgggag atctaatgat
     3121 gtatagcaag catcactaaa cgtatagaat atttgtatta ttataatatc aatatgctat
     3181 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
     3241 tattctgaaa acaaaaaaag acgcgagttt ccacaatctc actcatggaa actcacgtct
     3301 ttcaaaaaat agggtgaatt gaaaactaga acaagacact agattttcta aatgatgtct
     3361 agtaaggatt ctaaatgcta caccaaattg ctcacatctt atttcaaacc acgggactcc
     3421 cacaagaaag gaaaatccta taaaaattct ctctctgagg agaaaggagc ctcaactaca
     3481 ggtgtaaact attatcaatt gaaaacaaca gtttttacac tcaattagat tttttaagga
     3541 caggatcact ttcgcttgac tgtagtttgt catttttttg ataaagttag cactttttca
     3601 gaaactgttt tgaaagtctc aatgatatag tctacttctt catcgcttaa tttagtatga
     3661 agagggaggg taatttcatt ctcaaagaag gcataggcct taggatagtt cgtcatatca
     3721 aatccaagat tcttataggc tgtcaagaga ggaagcggtt tgtagtgaac attacttgca
     3781 attcctgctt tagccaattc ttggatgatg aggttgcgtt cttctaggct tgctccttct
     3841 acacgggtga tgtagaggtg gcgtgaagat tcgacagttt cagtcttgtg tgccaaagga
     3901 tggatgcgag aacctgcaaa accactatca tagcggtcca caatgtcctt acggcgttgc
     3961 aacaaacttg gatagcggtc caattgtacc aaaccaagtg aagccatgat atcggtcatg
     4021 ttgcacttat aggctggtgt aacgatatcg tattcccatg accccagttg catcttggca
     4081 agagcatcct tagtttgccc gtgaagggaa aggatttgga attccttgta catctcttcg
     4141 tcatcaatca ctggattggc tttccaagtc gcacttccac cttctgccgt tgtaaagttc
     4201 ttaacggcat ggaatgagaa ggaagtaaag tcagcgatag aaccagaagg ttgtccttta
     4261 taagtagatc ccaaagcgtg ggcactatca gagacaatga caatacggtt aaaggccttt
     4321 tgccacttgc ttgaagcggt aaagaagtca cgttttttct ccacgacttg gaacaaacgg
     4381 tcataatcgc aaacaatccc tgcgagctct actgggataa tcaccttagt tttctcagtg
     4441 atagcttgct caagcaggtc atagtccatc tcaaacgtat ctgcttggat atccaccatg
     4501 acaggggttg ctcccacgtg cgtaatgaca ctacatgaag ccgtataggt catggctgga
     4561 acgatgactt catcaccagg tcccacttcc aaaacgcgta aaatcaactc cagagcggct
     4621 gtcgcagagt tgagacaaac agtcttaggt gtctgtgtgt aaagagacaa gcggcgctcc
     4681 agttcttttg ttttaggacc tgttgtgatc caaccagaac gcagggtatc cgctacttca
     4741 gcaatttctg cttctgtgat atcaggcggt gaaaatggaa tattgtaatt tggcattgat
     4801 ttgctccttt tatctgtact cattttctta tgactacttt attttagtac ctcaaacacg
     4861 gtttgaaaca tgattttgat gtccccaaag aaactaaact ctcggagata ggcgaggtta
     4921 tagcgcatct tttcaggaag aacatgctcc acataggcct gatcaactga cagacctttc
     4981 tccgtcattt gactgatgat ggtgtcctca tccttgtagt tgatgctggc tggagaggta
     5041 attcctgctt gcaagagcaa ggttgccatc atttcagggc tatactgctc tgtataacgt
     5101 ggcacttcag gtcgtgtacc gacaaaggac atctcacctt taaggacatt gaccaactga
     5161 ggcagttcgt ccaaacggac acgtcggatg aaatttccaa ccttggtaat acggctatcg
     5221 ttagcagaag tcaccagact tcctttttta tccgcatccg tcaccatggt acggaacttc
     5281 caaatcttga accgacggtt gtactgggtc acacgctctt gcttgtaaat cactggcccc
     5341 ttgctatcca acttgatcca aatgctcaag atgagaaaga tgggagaggt tagaaccagt
     5401 aagaccaagg ccaaaaccca gtccaagcaa cgcttgaaaa tcagcgaacc cttcctttta
     5461 gagacaagct ggtagtaaga ctcaacctcg cttgatttca tttccacagg caagtcttcc
     5521 catttcagca tgctgttctc ctttgttttt attatactat ttgtcaacat ttttcattat
     5581 accacaaaat ggaaaaggcg gtgaaagaaa tctgtaattt gaaggatttc cttctagtac
     5641 tcaatgaaaa tcaaagtaca gctttgagat tgcagataaa gctgacatgg tttaaagaga
     5701 ttttcaaaga gtatcggcca tggcccctgc ctgcaaacta tacatcttgt gataagttcc
     5761 tcccagagcc aagagttcct catgggttcc actctcgata atgcgtccct tatccaagac
     5821 atagatgcag ttggcgtctt gaatagtaga aaggcggtga gcgatagcaa tagttgttcg
     5881 gccctgtctc atcttcgcca gagaagcttg aaccaaactt tctgtttcag agtcaatatt
     5941 ggctgtcgct tcatccaaaa tcaggatttt aggctggctg gcgactgttc tagcaaaggc
     6001 aagaagctgg cgctgcccag tagagaagct cgaaccacgc tcggaaacag gggagtcgta
     6061 cccctgagga agttcttgaa taaaggaatc tgcatccaca aaggctgccg cagcctgaac
     6121 ctgctcatca ctgatttctt ggtacatggc gatattggac ttaatagttc catgatagag
     6181 gaagggttcc tgcaagacca aaccgatgtt ttttctcagc tcttcttgac tgaaatccct
     6241 gatatccaca tcatccaaga gaactctccc tgactggaat tcataaaagc gcatgaggac
     6301 attgataata gacgatttcc ctgaacctgt atgacctaca aaggcaatgg tttcaccctt
     6361 attaacagag aaagaaatgt catccagaat cggatgttta ccgtcatatg agaaacacac
     6421 atgttcaaaa cggatattgc cttcttggac tttggcttgc ccattttctt gaagaggttc
     6481 ataggtcctc tcgtctatca gggcaaagac acgacctgca gaaaccatag ccgtttgcag
     6541 agttgaaaag ttttgcgtca cctcaatcaa ggggtcaaaa aggcggttga tgtactggat
     6601 aaaggcatac atggtcccga ccgttatccc gatagaaaaa ccacggtagc caaagtaggc
     6661 catcaagact gcatagccta gaagtttcag caaactcatg gcaggtctca aaaagagggc
     6721 atccaaggct acagaacggt tggcgtagac caagtgttct tggttgattt catcaaattc
     6781 tgcctgcagg cgcttctctt gattaaaggc ctgaataatc ctgattccct cgatattctc
     6841 tgccagctta ctattgatat ctgacaagag acttctggtt ttctcgatga ttttcactga
     6901 cttttttcga tagagattga ccaaaaggaa aatcaaagga agaaagagca agactaaagc
     6961 cgtcaaacga aaatccagca ccaacatggt ataaagggtt gtcagaaaga taaaaactgc
     7021 tgagataaag ctggataaaa tcccagaaaa catatcacta atcgtctcgg tatcgttggt
     7081 caaacgagaa acgatagaac ctgctggcgt cttgtcaaaa taagacatgc ccaacttttc
     7141 catattggca aaggcatccc gacgaatatc cctaacaata ctgtaagaca cgcgcgcaaa
     7201 gagaagattg ccgacatact gaactacagt ttgtaggatg tagagaccat agtagaccag
     7261 caaaacggta acggctagtt ggttaagatt gctgagatac tggtcgataa agtgggaagc
     7321 cacgaggggt atgacacttt taatgaccgt cgtcgctagg agaaagctga gtgccaaaaa
     7381 ggtcaggagt ctataaggct tgagatagga catcaagcgc ttcaatacag tccattgttc
     7441 ttgtttattc tgcatcttct tctcccttca tttccagctg ctgagactgg taggtttggg
     7501 cataccagcc atccaaagct agcaagtctt cgtgcctgcc tcgttcgata atttgaccat
     7561 tttgcaggac taaaatcaaa tctgcatgga caactgcact caggcgatgg gcagtgataa
     7621 tggttgtctt gtcctttcgc gtctccttga ggttgtcgat aatcgcatac tctgtcttgg
     7681 catctacggc ggataaggaa tcatccaaaa tcaagatatc agggtctaaa atcatagccc
     7741 gactcatagc caaccgttgc ttttgaccac cagaaagact gactcctttt tcaccaatca
     7801 gcgtatcaaa tccttgaggc atgtctacaa tatcttggta aactcgggct agcttagtag
     7861 cttcctcgac cgctgaaagg ggcaagttag gattgccaaa gcggatattg tctaggattg
     7921 aagtcgcaaa gagaaactgg tcctgaggaa catagcccat gagactgcga aggtctgtca
     7981 gacgatagtc ccgaatatcg tgaccgttta gataaatggc acccttatcc acatcgtatt
     8041 cacgcaagag gagcttgatt aaggacgttt tcccagagcc tgtctgccca accaagccca
     8101 gggtttgccc ttttgccaaa ctaaagtgaa tatccgtcag tgtttcctca ttttcaaaag
     8161 caaagctgtc aatggcatac tccaaacgcc cattttcaat accatccaga ggaaactcag
     8221 ggtcttgtac aggagattcc tgagacaaaa gattttcaat ccgctggtaa gaaaccttcc
     8281 ctcgctgagt agtattaaag aggaaaccga tggccagaag aggccagacc agcatatcca
     8341 aatagctgat aaaggtgact agattcccaa ctgtaatctg cccttcctga accatcaagg
     8401 agccaaccaa aagcgttaaa acataggacg aaccaacaaa caagagaacc atagggtcaa
     8461 agagactatc atatttcatg gtttgcaggt tcttttggaa ggttaattca ttgactgcct
     8521 gaaaagactt caactcgtct gcctgataac cgaaagactt ggtcactttg atacctgata
     8581 cggactcctg taccttgtta ttgagttcag aaaaagcagt ttgggattcg ccaaaggcct
     8641 tatgagtctt tctccctagg cgactagtcg cataggccat gaaaggtagg gggagaatgg
     8701 caacaagagt catctgccat gagatgctaa agagcatggt caacaaagtc accagagccg
     8761 tgatagaggc atccaccgca gacatgacac cgccacctgc taaacgagtc aaggcattga
     8821 tatcattggt tgcgtgtgcc atcagatcac ccgtccgata ggtttgataa aaggctgacg
     8881 acatttttgt gaaatgctta aacaagcgag accgcatgat ctgtcccaag caataagagg
     8941 tcccaaggat atacatacgc cacacatagc gcaaatagta cataccaaag gctgcaagta
     9001 gcaagtaaaa taggctaaga aggaggtcct gctgggttaa ttgccccgat gtgatggcat
     9061 caataacccg ccccataacc ataggaggaa tgagattgag gacggaaacc aagaccaggg
     9121 ccacaattcc gactagataa cggcgttttt ctaacttgaa aaaccaccaa agtttttgaa
     9181 taatggacat aaaatccctt tctgattgca aatggaaacc tgaggccaat actcaatgaa
     9241 aatcaaagag caaactagga aactagccgc aggctgctca aagcactgct ttgaggttgt
     9301 agatagaact gacgaagtca gtaacctaca tacggcaagg cgacgttgac gcggtttgaa
     9361 gagattttcg aagagtacaa gaccccaggt ttttcttatt cataagttac gactgtaaca
     9421 gcacccttgt catattcagc aataaagata ttggctacat tgtcatgccc ttgtttactg
     9481 aggttatcaa gcaaccactc ttcgctacga ccaatcgatt ccaagacatc tacttgaatc
     9541 acaccgtcag tcacaactgg atacttagga ttttcatctc ccatttgcac aacgatgagt
     9601 tgcccatttt gctcttgcac agctcgtttg acttgcttca tctggaaaat cccttggcta
     9661 cgaagtttga gggctacatc cgatgcagac aaaccaactg aacgacaggt ttatggggca
     9721 atattcccat ttttgataag gagagttggc ttaccatcaa tcaaacgttt cacaaagtga
     9781 acattgttgt tgagccattt gagagtcaag accagtttga ttaaaatttc gatataatta
     9841 agtgtcattt attcacatcc accagttcaa ttgcgtctgt cttatacaat tcgactttct
     9901 ctaacaggta cttgtctggc tcacttccgt ttagggcacg ataatagcga tctcccacct
     9961 tgataagtgc gccatctgtg tttgtggaag tattaacata gacttctgac ttgtctactc
    10021 ccaaatcttt ggaaacaacc tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa
    10081 cttgattgtt ttgataagtc gtgatgctga ccaaaagcag agctaaataa ggccagagct
    10141 gaaatcatga ccaactcacg aaattcggtt ccctttttat catggtatgc cttaaaagca
    10201 aaaaaactgt cacagctaat agaacaatcc caaagccaac catgatacca ttttgctgac
    10261 cgatttggct gagtacatag tcataagagt agaatttcat gtattttcac tccctttcat
    10321 aaaatcattc ttaattataa acgaaagaga gtaatttttc aaactatacg ttggggaaat
    10381 agatcttttt ttggtataat aaaatctata atctgaatga aaaaggtaac tttatgaaac
    10441 ttatctcatg gaatattgat tccctaaacg ctgccctaac tagtgactca gctcgtgcca
    10501 aattgtccca agaagtccta caaaccttgg tcgctgaaaa tgctgatatc attgctatcc
    10561 aagaaaccaa gctttctgcc aaaggaccta caaagaaaca cgtggaaatt ttagaagaac
    10621 tcttcccagg ctacgaaaac acgtggcgtt cttcccaaga gcctgcccgt aaaggctatg
    10681 ctggaaccat gttcctttat aagaaagaac ttacacctac tatcagcttc ccagaaatcg
    10741 gtgccccttc taccatggac ttggaaggtc gtatcatcac tctagaattt gatgcatttt
    10801 tcgtaaccca agtttacact ccaaacgctg gtgacggtct caaacgcttg gaagaacgcc
    10861 aagtctggga tgccaaatat gctgagtatt tggctaaact agacaaagaa aaaccagtcc
    10921 ttgcgaccgg tgactacaac gtagcccaca atgaaatcga ccttgcaaat cctgctagca
    10981 accgccgttc acctggattt actgacgagg aacgtgctgg atttaccaac cttttggcaa
    11041 ctggatttac agacaccttc cgccatgttc atggcgatgt tcctgaacgc tacacttggt
    11101 gggcacaacg cagcaaaact tctaaaatca acaatacagg ctggagaatc gactactggc
    11161 tcacaagtaa ccgtatcgct gacaaggtca ctaaatctga tatgattgac tcaggtgctc
    11221 gccaagacca tacaccgatt gtcttggaaa ttgacttgta aggattttct atatggacta
    11281 caatgcggtc attcccgagt tgcttgtatc gaatatcgaa cagtcccggt ccttctactg
    11341 tggtttactg ggttttagga ttgaatacca acgtccagaa gaaaactttc tctttctctt
    11401 gaagagtgtc aactaatgtt agaggaggga acaaaggatc aactagctga actgacctat
    11461 ccctttggtc gtggagtcaa tctctccttt ggcataaagg atgtttcaaa actctattaa
    11521 agagtgatga aggcaaacta tcctatttat cgtcccttga ccaagagaaa gtttcgtgtc
    11581 agtgatccct atatctatcc tcatgaattt gcagttttgg atccaaatgg ctatttttta
    11641 agatttagcg agtagaataa taaaaattgg gaaaagtctt taaagttaga aaaaggcata
    11701 ttgtcaggtg tctaaaaact tgatagtatg ccttttatga tggaaaaatc ttctaatact
    11761 attcgaaaat ctcttcaagc cacatcaacg tcgccttacc gtaggtatat gatactgact
    11821 ctgtcaattc tatccacaac ctcaaaacag tgatttgagg ttgtggatag cttcctagtt
    11881 tgctctttga ttttcattta gtataaatgt tagcttacaa cggagttttt gttcaccccc
    11941 tcttctgttt gttcgttttt taaaccaaaa cactaacaaa atagttgatt tataaagtta
    12001 tttagtgtaa aataaaaaca ttggctcaag cctatttaaa attgaatatc gttttacttg
    12061 tttatgtcgt gaattggcac gacgtttcta caaggtgccg gaacacctaa caataagtaa
    12121 gtcagcagtg agatatggcc gtttttgcca tgtctattta ggggacttac ttagagatta
    12181 agtaggtctt ttttgaatat ttttaatatt tcccttaatt agtgcggacg ggaggtgaaa
    12241 ttatcctgtg aataatttca ggaatccagg gaatttcatc cctaattttt gagcccctag
    12301 ctctttcatt tctaggctta ggctaaaata gttccccgaa ctattttact ctcaaaaatc
    12361 ccgtcataga caataacaag attattgtct atgataccac tacaacggga aatatttgat
    12421 aagactcgct tcgctcgtaa tagttttatc aaatatatag tattagcaaa agggatcact
    12481 atagaaacat ttaaacaaca agtaaattag cttttagact ttaggaggtc ttatgaaatt
    12541 attagaagag cgcatcctca aggatgggca tatcttgggt gataacatcc tcaaggtaga
    12601 ttccttttta acccaccaag ttgactttag cttgatgcga gagattggta aggtttttgc
    12661 ggaaaaattt gctgctgccg gcattaccaa ggtcgtaacc attgaagcgt cgggtattgc
    12721 cccagccgtt tttacagctg aagccttaaa cgttcccatg attttcgcca aaaaagctaa
    12781 gaacatcacc atgaacgaag gcatcttaac tgctcaagtc tactccttta ccaagcaggt
    12841 gaccagcacc gtttctatcg ctggaaaatt cctctcacca gaggacaagg ttttgattat
    12901 cgacgatttc cttgctaatg gccaagctgc taaaggcttg attcaaatca tcgaacaggc
    12961 cggtgccaca gtccaagcta tcggtatcgt gattgagaaa tccttccaag atggccgtga
    13021 tttgcttgaa aaagcaggct accctgtcct atcacttgct cgtttggatc gttttgaaaa
    13081 tggtcaggtc gtatttaagg aggcagatct ctaatgcaaa ctcaagaaaa acactcgcaa
    13141 gcagccgttc ttggcttgca gcacttacta gccatgtact caggatctat cctggttccc
    13201 atcatgattg cgacagccct tggctattca gctgagcagt tgacctacct gatttctaca
    13261 gatatcttca tgtgtggggt ggcaaccttc ctccaactcc aactcaacaa atactttggg
    13321 attggactcc cagtcgttct tggagttgca ttccagtcgg tcgctccctt gattatgatt
    13381 gggcaaagcc atggtagtgg cgctatgttt ggtgccctta tcgcatctgg gatttacgtg
    13441 gttcttgttt caggcatctt ctcaaaagtg gccaatctct tcccctctat cgtaacagga
    13501 tctgttatta ccacgattgg tttaaccttg atccctgtcg ctattggaaa tatgggaaat
    13561 aacgttccag agccaactgg tcaaagtctc ttgctttcag ctattactgt tctgattatc
    13621 ctcttgatca acatctttac caaaggattt atcaagtcta tctctatttt gattggtctg
    13681 gttgttggaa ctgccattgc tgctactatg ggcttggtgg acttctctcc tgttgcggta
    13741 gctccacttg tccatgtccc aactccactc tactttggga tgccaacctt tgaaatctca
    13801 tctattgtca tgatgtgtat catcgcaacg gtgtctatgg ttgagtcaac tggtgtttat
    13861 ctagccttgt ctgatatcac aaaagatcca atcgacagca cgcgccttcg caacggttac
    13921 cgcgcagaag gtttggcggt acttctcgga ggaatcttta acaccttccc ttacaccgga
    13981 ttttcacaaa acgttggttt ggttaaattg tcaggcatca aaaaacgcct gccaatctac
    14041 tacgcagctg gtttcctggt tctccttgga ctgcttccta agtttggtgc ccttgcccaa
    14101 atcattccaa gctccgtcct cggcggtgcc atgctggtga tgtttggttt tgtatctatt
    14161 caagggatgc aaatcctcgc ccgagttgac tttgctaaca atgaacacaa cttccttatc
    14221 gcagctgttt caatcgctgc aggtgtcggt ctcaacaaca gtaatctctt tgtcagcatg
    14281 ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tagccagcct actcgctatt
    14341 gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg agcctctttt
    14401 ttgtgtgaca aaataaaaac atctacctat tcatatatgc tagtgtcata gtcctgaaaa
    14461 tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag tcgttcggct
    14521 tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt aatttctact
    14581 gtatactttt tcagttttag attgctttcc aataattcac ctattaacaa taatctatga
    14641 taggagctat gtagccactc tggcaattta tctcctactt cccacccata tttttccttc
    14701 atcaaagcta gttgtttttc gtcaccaaat ttcatatttt cagataacca tctcacacaa
    14761 tcgggatctg ataaggtaca gacgattaaa cttaggttat cgtaacggct ttcagtttca
    14821 taatcaggcc agatgacttt taagaaatca tacacctctt ctgatgcaac aagttgtttc
    14881 gttttcataa tttccgatac tttcctctac ctttaaattc tattatttct ttgtctctta
    14941 atatttgaag ctgttgccta atcttttctt tgatatgatt gttcttaaca aagatatttt
    15001 ttaggtcact ttcaaaacga tacatatctt caagggtaaa ttctgaaccc tctatcttat
    15061 ctatacaatt tagaatttct attgtccaac ctcttgatga cagagagctc ttccttaaaa
    15121 ataaaccttg cttaaattct tttgtaactt tttctggatc tctaacttgt ccatcttgca
    15181 caagaaatat ccttccttta gaaggtactt gtgataaatc aatgttacaa ccaatccaac
    15241 ctgctcgtct agcagttggt gcaagtggtt ttctttgaat aatcgatttc ggtgtaacaa
    15301 attgcttcgg aaggacaaga aagttattta cctcaaaatt ttttgtgtaa gttaaaaaaa
    15361 agaaattagg attattatct gcctgcacac gcttcatcat cgttgcataa gcaccatcat
    15421 tgattgttga tgaaaaattt ccttttttgc tctttagttc aaactcctca ctacaatgat
    15481 tacagtaaaa atctgctaca ggccgattat tttcaaaatg atttaaggga ttgttcccac
    15541 aattagggca ataagactga cgataaaccc aatcctccgt taagatacgt gccttttggc
    15601 tattactttt ataggtttct actaattcta aattaaagtg taattccatt taaaaattcc
    15661 tcactaatat gagaactcac tcgatataag caattaaaat ctactataac tagtatacca
    15721 catatatgta cctattacat ttgtatttca ccactctctt ttatatttcc ttctaacata
    15781 tctcataaat taaaaatgag tgagataaat ttatcccact cacctttctt tatatcctta
    15841 cccgtctttc ctcagacctt tggcctgtga caaacatggt aaaggttgct ttacagacat
    15901 ttctgccctc ttgattggtg atatccacat ccatcacaca ggttgtacga ccttgatgga
    15961 cacattctcc tttaatggtc aatacatcgt cgagttttcc tgccttaagg tagttgatag
    16021 aggattggag tgtcacccca tcaagtccca gcgagataac caccaaacca ctgatttggt
    16081 cacatagcgt gaatagatag ccaccatggg cattgccata gtagttgagc gacgagttca
    16141 ctactttggt cgtcaccaca acgtgaccat ctctcatttt ttcaatttcg taattttcaa
    16201 aggcagatat agcgtcaaaa tgaaagtctt tcatcgtttc ctcctgatat ttcaaacgac
    16261 actatgatac tactttttat aggactgtgc aaggagaaag ctcctagtct agtgaaattc
    16321 cgacctgttc cacaaagcgc ataaaggctg tctgcccttg ttctgtctgc ttgtagactc
    16381 ctgcatcctc aagtacacgc acaaagatag cacccacaga gtccttgacg attgcaaggg
    16441 ctttttcttt atccgttagg tctggatgtt gggatttgag ttggtctgcc cactcctgat
    16501 gataatcggc aactgtaaca gcttctccta caagatagct agcgacttgc tccacttctt
    16561 ctttcagacg tggtggcaag attgccaagc ccatgacctc aatcaagccg atattttcct
    16621 tcttgatatg ttggacatcc ttgtggggat gatagatgcc atcaggatgc tctgctgaag
    16681 tctgattgtc tcgcaagacc aagtccaact caaactgtcc atcacgctta cgcgctattg
    16741 gtgtgatggt gtgatgcggt gtcccgtctg tctctgctaa aatctgcaca ctagaatctg
    16801 aatactgacg ccattcctgg aaaatcttat cagctaagtt aatcaaatcc tctttggaat
    16861 ccgaagtcaa gcgtaggaca gacattggcc acttgataat tccagccttg acctgctcaa
    16921 aaccagcaaa tcggaaggtc ttttgcaagg gagccaattc cataggaaat acgtgacggc
    16981 ctccctgata atgatcgtga gttagaatag agccccccac aatcggcagg tcggcattag
    17041 atccagcaaa atatcctgga aactgctcta cgatagccaa cagacgttca aaactctgac
    17101 gactaatggc catgggacga tgctggccat ctaaaaagat acaatgctca ttaaagtaag
    17161 catagggcga atactggaac ccccattcct gaccaaccat ttcaaaacgg ataatccggt
    17221 ggttgctgcg agctgggtgg ttgacccgac catggtaacc ctcattctct aaacaaagtt
    17281 gacactgagg ataattacta gcttgcacca acttggctac cgcaatctct ttcggatcct
    17341 tttcaggctt agagagattg atggtaattt caagttctcc ataatcagat ggaacacgat
    17401 aagcgatatt tttagcaatg gccttgagtt tgatgtagtc atttttctga ctgagttgat
    17461 aaaaatcctc tatcgcttgc tcaggagagt aggcataggc ttcccagaag tcacgattga
    17521 cctgactcgg acaaggagtc accaagtcca tcagttcagt accaaggatt tcacgcgcag
    17581 tctgactatc ctcaatcgtc tctaatcgaa cggcttcctc aactagctgg tccttgaggt
    17641 caatcaattt atccagattg gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa
    17701 cacgattggt caggtagatt cgatccattt cctcaaatga actttcagaa atgacatgtg
    17761 ttacaaattt atctactaag gtcactatag agcctccttt tgactagtca aggacgcgag
    17821 tgccacctgc aacttcagcg atatagaagc ttggagcgta tccaactact tcctcgtagt
    17881 gtttgcctac agcttcctta aaggcctcaa cagtatcttt ttgaaccaag gcaatggcac
    17941 agccaccaaa accagcccct gtcatacgag caccgagaac tccttcttgt gcccaagctg
    18001 tgtgaacaag ggtatctaat tccaaaccag ttacttcata atcatgctcc agagaaacgt
    18061 gtgacgcatt catcaaacga ccaaatgttt ccaaatcacc tgcttgaagg gctgcttgag
    18121 ctttaagggt acgttggttt tcaagcacag catggcgagc acgtttcaaa cgattttcat
    18181 ctttaattag atagctatat tggtcaacgg cccactcgtc caattcaccc agggtctgaa
    18241 tatccaaggc aacttgcaat tcttccactg ctttttcaca ctcagcacga cgttcattgt
    18301 atttagagtc cgccaattca cggcgtttgt tggtgttcat gataacaacg acattgtcct
    18361 tcaaatcaag tggcaccaaa tcatactcta aggtgttggt atcaaggtaa atagcacgtt
    18421 gatcagcccc cataccaata gcaaactggt ccatgatacc agagttgact ccgataaagt
    18481 tgttttctgt ttgtttgccg attttaacca aatcgagacg ctctaatttt aaatcaaaga
    18541 gatgctctgc cacgactcct gtcaagagct ccaaggatgc tgaagaagac aagccagcac
    18601 catttgggat attcccataa acataaaaat caaaaccttt gtcaatcacg tgcccagctt
    18661 cttgcaagaa atgaagaacc ccttttggat aattggtcca gttgtgctct ttttcaaact
    18721 tgaggtcagc gagaggcact tcgataatgc ccttgtcctc aaagttagct gagtagaaac
    18781 gcaagacttg gtcgtcacgc ttacgagctg caccgtaagt tcccaaggaa atagcagcag
    18841 gaaaaacgtg cccaccgttg tagtctgtgt gttcaccaat caaattaata cgacctggtg
    18901 aaaagaaggt ttggtctgct tcttgaccaa aaacagcaag aaagtctttg cgaaggactt
    18961 cagtagtaag atgttgtgcc atatgaattc tcctttgact gtccgttaag ccatcttaac
    19021 gtgtaatcgt tttcttctat tgtatccaag gaatttccca aggtcaatcc tttttactaa
    19081 aattttagta aactatcagt aaaaagaaga aaaatatgat atactaacct aatcaaggag
    19141 gatttatggc taccttaaaa gacattgcac agctagcctc tgtctctatc gcgaccgtat
    19201 cccgtgtcct caaccgcgac cagagcctat ctgttacaga agaaaccaga caccgtattt
    19261 taaccgttgc tgaagagctg ggctacacca agcacctcaa gacaggcgag tcccataaac
    19321 ccaagcaaaa gattgccatt attcaatggg tcagcgaaca aggggagcta gacgacctct
    19381 actactacca gattcgccta ggaatagaaa aaagagccca agagttggac tacgatatct
    19441 tgcgctattt taatgaccat ccttttaccc taagcgagga agtgattggg attctctgca
    19501 tcggaaaatt tagccgagct cagatttctg cctttgaaga ataccaaaag cctcttgtat
    19561 ttctagacag cgatacactt tccctgggac atacctgtat tatcacggat ttttacactg
    19621 ctatgaaaca ggttgtcgat tatttcctca gccaaggaat ggaccgtatc gggattctaa
    19681 caggccttga agaaacaact gaccaagaag aaatcattga ggataagcgg ctggaaaatt
    19741 tcagaaacta cagtcaagca aaaggaatct atcatgatga actgatcttt caaggaaact
    19801 ttactgccca gtctggctat gacttgatga aggaagccat tcagaacttg ggagaccaac
    19861 tcccaccagc atttttcgca gccagcgata gtttagccat cggtgccctt cgtgcgctcc
    19921 aagaagctgg aatcagccta ccagaccgtg tcagcctcat ttcctttaac gacaccagcc
    19981 tgaccaagca ggtttatcct cccctctcca gcatcacagt ttatactgaa gaaatggggc
    20041 gagcaggtat ggatattctt aacaaggaag tcctccacgg tcggaaaatc cctagcctga
    20101 ccatgctggg aaccagactg acattaagag aaagtaccct aaatcaagaa taggataaca
    20161 taaaaaacga atagagttct aaaactccta ttcgtttttt attcgattac aatcatagac
    20221 ttaattgtct tacgttcatc catatcttta taggcttggt cgatatcttc cagtttataa
    20281 cttgaagtaa agacgcgacc tggattgata tcaccatcaa ggacggcttt tagtaaaaat
    20341 tgcttatcgt atgttgtagc agaagctgcc ccacctgcta cagagatatt ttgcataaat
    20401 gtcgaaccaa gagcacgatt attatagtgt gggactccta caaagcccat acgccctcca
    20461 ttatgaagaa cacctagcgc ctgttctata gcagcctccg taccaacaca ttcaagtgct
    20521 gcatctgctc ctccaccgag gatttcacgc accttggtaa ttccttcttg accacgttct
    20581 gcaacaacag ctgtcgcacc tgactccata gccatctttt gacggtcttc atgacggctc
    20641 ataaggataa tttgtgatgc tccacgcatc ttagccgcga tgacagcaca ttgaccaaca
    20701 gccccatcac cgataacaac aaccttgtcc cctttttgaa catttgcaac acgcgccgca
    20761 tgatagcctg tcggcatgac atctgcaaga gtcaaaaggg acttgagcat cccttctgta
    20821 tagtcagaag gttgaccagg gattttaacc agcgcccagt ttgcatagtg gaagcgaata
    20881 tattctgcct gaaaatcacc ccccaaatta ttgccaatat gattgtcgca agaaccgtca
    20941 aatccagcaa gacaggcatc acactcacca catccatgtg taaaagggac aatcacaaaa
    21001 tcacctggtt tcaccgtcgt aatggcttcc ccagcttctt caacaatccc aatcgcttcg
    21061 tgtccactat ttttgtgtcc agctttcgtt tctggattac ggtacctcca taaatctgaa
    21121 ccacaaacgc acgcacgaac cacacgaata atcacatcat ccgcttctat tatttgcgga
    21181 cgcaagtcca acctgacctg cctttgtata tactgctgat ttcatttaaa attttccttc
    21241 cttataaagt ttaattttga gatttaaacg atttaaagct gtctgtagct gagacaagcg
    21301 ctcctctaat ttttgctttt cctcttctaa aataccaagc ctctcctctc tcgtttcatc
    21361 tcccttttgg tagagcgaca tatagtcaac taaactatct acagagacac ccgccgaacg
    21421 aaaacactta ataaattcca gcgcttcgat atcctgatct tgaaaatcac gaatcccagt
    21481 agcagtacga gtaatcggtg gcacaagacc aacccgttca taataccgaa tcgtatccgc
    21541 tgaaattccc aacaaatcac tggcagattt aatattcaca cgctaacctc ctctcatttc
    21601 attcactgaa ttcacgctat tctaactcac ttatcttatt atataacttt gagttgactc
    21661 caagtcaagc attttgtatc taatcgaaaa atttttatca ttaacaatca tatatctgga
    21721 gtttatacaa tgctcttctc ttaccaattt ttgaaatatt taaataataa agaaattact
    21781 ctattccaaa taagatagta cccaagtatt tttcactttt ttctaatgtt gatgctcata
    21841 actccgttcc aagtcacaca cctttcgctt atgggtttgg tgagtttcta ggtcagcatc
    21901 aatttcaatg gtaatatttt gaaaaccaca atcttttagg aaaattcgaa tagactcttt
    21961 acaagtttcc atatgttcca tttcttttag acaaacatgg acaatggcat ttttttccaa
    22021 agcatccata gtccagagat taagctgatt aaggctggcc acattgtcca atcgctccag
    22081 gtcactcttt acttgcttga tatcaagacc ttctggcaca gcatccaaga aaatcttgag
    22141 tgtagaccaa aaacgtggaa gggcttttga aagaataaag aaagaaatga caagggacaa
    22201 aagaggatct aggatatacc agtccgtaaa tcgaagaaca atcgccatca ggataacagc
    22261 tacccaccct agcgtatctt ccagaaaatg cagactcaga atagactcat tctttgtctt
    22321 tcccttacca accaccagac tcgctaacag attgatagta atcgcaataa ttcctaacca
    22381 gagaatcccc tcatcattga ctggttgcgg atgcaaaatc ttcgtgacat tttccaaaat
    22441 gactagaaca gagcctgtta cgagaatcac agctgttacc acggctccta gcaggctaaa
    22501 ccgcttatag cccaaggtgt actgattgtc ttcttcacga ttggagattg tttctagaaa
    22561 agctgatatt ccaattgcaa tcacatctcc caagtcatgc acagagtcag caagaacagc
    22621 gctagaacca aatactccac ctgcaataaa ctcaacaatg gcataagtca aatttaagaa
    22681 aaaagccacc caaacagcat attttgcctt catatttctc attcctttgt tataatagat
    22741 ttatgaacac cttgttcatt atcattatcc actaaaactc aaagaaagga tagaagcaaa
    22801 acgtcagctt tattcagttc tgaacaattt gcctcaagtg tccatatgac taacattgac
    22861 cgccgtatca gcaaaaccaa aaaagccatc tatcaagctt ttatacaact tttgaatgct
    22921 aaggactacg agaccactac tgttcaggat atcattgatc tcgcagatgt gggacgatcc
    22981 accttttact gtcactatga gagtaaagag cttcttctgg atcagctctg ccgctacctc
    23041 ttccatcatc tttttgaaag agagcaagcc atttcaaccg aggactacct cgcccacctc
    23101 tttctccatt ttcagaaaaa ccaagaccat atcgccagtc tgctattttc caaaaatgac
    23161 tatttcctcc gtcaactaca gagagagctt gagcaccacg tctatcccat gctggctgct
    23221 gatttgaagc aaactcaccc aagtctacct acttcttacc tccaacattt tgtcgtaact
    23281 aactttatcg aaacattgac ctggtggctc aaaaaaggtc aagatttcac agaccaggaa
    23341 gttgtccaat tttatctaga ccttctcatt cctaaaaatt gaatatagag taaagcttca
    23401 gttgtcttat ttctaggtta ctgagttttt tatcttttca acaacaaaag aggacccgcc
    23461 gatcctcttt ttcatactat aaatccttga ttatcaacta tatctgtttt aatcgaaatc
    23521 tcaaaacagc actttcaaac atcttttcct agttaagtaa atcagtattc tgcttagctg
    23581 ccttgctcca ttgataccaa ccaactagac tgttaatgag ataaattaga tatttccctt
    23641 gaatttgcag gctttctccc caccagagat agattgaaaa gacattggta gccgcccaga
    23701 atatccactg ttcacggtaa acagctgtca tgaggatttg ccctacccca ttggttgcat
    23761 ctgtgattga atcacgatag ggacgattgg caccaataga ctgataaatg aagccaaagg
    23821 ccaaccacca aagcacacta atggaaagat actttgtcca gcccttgccg tccagtttac
    23881 gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg ataaatccaa actagaagtc
    23941 caattggctg catgactgtg aagtaaagtg tcgtcagcac ctcaccataa aagcctttct
    24001 gtagggccaa aataaggtaa ataacagagt taatcaagcc aaaaagataa ttacttgctc
    24061 gaccttccga tacaaagatt acacagataa tccctgtcaa gctacaaatc atcccaatcc
    24121 agtcaacaat acgatgttcg taaaccaact ccagccagag aggaaaactt cctaaaacca
    24181 gcaaataaat ccactgggca aaactacgat gggcaaagag gtcatcccag atagccttca
    24241 tagttcctga aaatcctaaa tcagccatag ccgcaaccat acgacggtaa ccacctgaca
    24301 tttcacctag ggttgttttg atattttcaa ttttcttttg caaataagta tgcatcattt
    24361 ctccttttgt ttttaaagag ccgtgtctgg atagactttc ggacgcaacg ctctattaga
    24421 taatgaactg cctatacaca agatttctaa ccttagtcga catgagctga aaccccttat
    24481 ttgttaagta gttcacaaaa tattatacac ctattttatg aatagtcaac tgtctttaca
    24541 gtaaaatttt agaaaatcat gaaaattttc tctttctttc cattttaagt gacattcagt
    24601 cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc attcttttat ctagtcgttt
    24661 aaggaagttg agttcagtat gtttaaagtc tctgtcccat catttcttca acaaaccttg
    24721 ttcttggaga aactccttgg ctacttgctt tgctgacttg ccttcaacac cgacttggta
    24781 gttgagctgg ctcatctggc tttctgtaat cttaccagcc aatgtattaa gaactctttc
    24841 caactctggg tgtttcttga gaagagcttc tttcatgagt ggagcccctt gataaggtgg
    24901 gaagagttgc ttgtcatctt ccaagacctg taaatcataa cgctccaatt ccgcatcagt
    24961 cgaataggcg tccgtaattt gaatatcacc tgactgaata gcctgatagc gaagggctgg
    25021 ctccatggtc gctacattaa gattgagacc atacattgat tgcaagccct tatttccatc
    25081 ttcacggtcg ttaaactcga gtgtaaaacc tgccttcaac tgcccttcca cttttttcaa
    25141 gtctgaaatg gtcttcaagc catattcttg agcaatcttt ttcgaaacag ctacagcata
    25201 ggtgttttga taagacatgg gtttgagata ggctagatga tcctgcttag caatgccatc
    25261 acgcgccacc tgataaacct gttctggttc atgactcacc ttgggtgatg gttgaagcaa
    25321 actttcagtc accgtaccag taaattcagg atagatgtca atatcgcctt ttttcagagc
    25381 ttcataaagg aagcttgtct tcccaaaatt cggtttaaca gtcgcagtca tgctggtatt
    25441 ttcttcaatc agcaacttat acatattggc caaaatttct ggttctggac ctattttccc
    25501 agcaataacc aagttttcct tctctttttg aaccaaaaga gctggactat aagacagacc
    25561 cagtaataaa gccaccaagg caaaacctga gaaaatcgtc cgtaattttg ctttttccat
    25621 tacttttagt aggaagttaa aggcgatagc aagcactgca gaagaaagtg ccccaatcaa
    25681 aatcaaactg gcattattac ggtcaattcc caacaggata aaggaaccca gtccccctgc
    25741 tccaatcaag gccgccaagg ttgccgtacc gataatcaaa acagctgccg tccgaatccc
    25801 agacatgata acaggcatgg cgagtggaat ttcaaatttc ttgagacgtt cccatctggt
    25861 catcccaaag gcaatcccag cctcttgcag gttcggatca attcccttca gcccagtgat
    25921 agtattttgc aaaataggga aaatcgcata aatcactaga gctgtcaaag ccggcaaggt
    25981 cccaattccc atcaaaggga taaagagccc caacaaggcc agagacggga tggtctggaa
    26041 aataccggca atctgcaaga cccagtcggc cagcttctca tgatagcgaa gaaaaacagc
    26101 caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa agtgacaact gcaaatgttg
    26161 agatagagct gtcaaccaat cactaaaacg atcctgaaaa gttgcaatta aattagtcat
    26221 gaacactacc tccaaacaag tctgctacaa agtctgttgc aggcactttt aaaattgtct
    26281 cgggattcgc tacctggcga atttctccat cctgcaagac agcaatacgg tccgccaact
    26341 tcaaggcttc atccgtatca tgggttacaa aaatcgttgt catcccaaac tctttatgca
    26401 attcttttgt cagaacctgc aactgttttc tcgaaatagc atccaaggcc gaaaagggtt
    26461 catccatgag gaaaatcttg ggctgaccaa tcatagctcg gacaataccg acccgttgct
    26521 gttctccacc agataattca ctaggtaagc gatgcccata ctcggctact ggtaaaccaa
    26581 ccttagccaa aagctcttct gttttcttcg taatttcttc cttgctccac cccttcattt
    26641 caggaatgag agcaatattt tccgcaactg ttagatttgg aaaaagagca atagcctgta
    26701 aaacataacc agtagaaaga cgaagttcac gctcatcata gtctttgatg cgcttcccat
    26761 ccatataaat atttccatca gttggttcca aaagacggtt aatcatcttg agcatggtcg
    26821 tcttacctga tccagaaggc cctactaaaa ccataaattc cccatcctca atctgtaagt
    26881 tgacatctct caagacatcc ttttctgtgt agcgcagtgc tacatttttg tattcaatca
    26941 ttctttgtcc tcaatttaaa acttccctcg attggtcaag tcttctacct taggcatgac
    27001 ttccttatta tcccaatgct ccacaatttt cccgttctct aaacggaaga tatcgtactg
    27061 ggcataagca acgccatcaa tctgagtctg accatagcta accacatagt ttccttgtcc
    27121 taagagttgg aaaacaaagt caaaagtgac actatattca gccacatagt ttttataagc
    27181 agcacttcct tgtccaatct catgattatg ctgaatcaaa tcgtctgcca cataatcact
    27241 ccactgctct agctccccat tttggaaaat ttctgtcaag aaacggcgaa ccagcttttt
    27301 attttctgct ttcttatcca aatccttgat ttcaaaatct ccaaaaattt gatctagttg
    27361 atcattttca ggtgttcgat agtagtcaat gacatcccaa tgctcaacaa tacaaccatt
    27421 ctcatccgca cggaaagtat ccgtcgtcac ccattgagct tctccaccat tcagatattg
    27481 atgaacatga acaaagacca gattgccatc ctcaatggtg cggacaatct taatctgacg
    27541 ctctggatga cgctcaaaga aatctgcaaa gaaggctgca aatccttctt tcccgtcagg
    27601 aacacctgtc gaatgttgga tataggtatc ccctacagac tgggcttgag cctcagcaac
    27661 tcgtccgtct tgaatggcat ggatgtatag gttgtgagca tttttcactt gttgtgacat
    27721 attctaaacc tcattttcct tctctttcag atttgccaaa attctttctt gaaaaccttc
    27781 aaattggtga atttcttcct ctgaaaatcc tttgtaaaag atagtatcca atttctgact
    27841 gacacgatgc cccacttctt tctggaactt gcctaactcc gttaaaacta aatacttctt
    27901 acgcttgtct tttccacacg gactaacaat tacaagcttt tgttcctcta gcttttttat
    27961 catagtcgtc agcgtattat tcgcaagccc agtcgcaagc gcgatatctg tcgcagttgc
    28021 gcagccagtt tcactattcc ataaaaccgc taaaatcttg ccctgttcac ccctataaag
    28081 agcctcagga tcttgactca gtaacttttg aaaaatccgc ccattcaaca aacgaatatg
    28141 atgggctagc aaatgactat ctttcataac acctccaatt tatttcgata tcgaaatgaa
    28201 taaaataatt gtaacactca tcgttctaac tgtcaactat ttcgatttgg aaataatttt
    28261 tgataattat tcacaccacc atactccggc tcaactaact tttaacgaga gtttctaaat
    28321 tccttcgtcc tccagtctac aaaagccttc catccgtact atcctatttt ttatgagggg
    28381 acacattttt cctatcagat catttatttt aaagatagaa gtaaatcata attgcttcca
    28441 tctgttcttt tatagtatat tgaagttgga ctagggcact gtatcttcta aaacattgat
    28501 agaaagcgat ttgaatttcc caatcaattt gttcgtattt atagcatttt gaaactagaa
    28561 taggacgcca tgactgctaa aagatttcta taaattcatt tgatttccct aatcaatttg
    28621 ttcatatctt atttcattcc gctataattt caccttaccc tatctttttc gtagcaccct
    28681 tcaaacagcc tatcccctac cgtttgacga ttcctcactt cactccactt ccattacaga
    28741 agtttcttca ctactatggt ctcggctgac ttctcatgat tccttgttac tactatttga
    28801 acgctcacga gataggtctt ataaaaaatg ctttgatcca caatggaatc aaagcatttt
    28861 aaaaagttcc tcatacataa gcgcagaagt cgcagttcct ctgtacttgg cttcttctct
    28921 tttgacaaag cgagccaagt tgagcaactc aggtgctgga tgtttgggat ttaggagcaa
    28981 ttcacgattg accaggcttg agagacgaac tgcctgcaat tgctcatttg tagtaggcag
    29041 ttttttagta gtctctagga gagcagcaac taaatcttca ctcaaatcat gtcgagcatg
    29101 attgtaaaga tcttttataa ggctttctag gtttggttct accatcccta ccacctccct
    29161 tatggtttaa taatgtttaa tcaaatcaac cgttgaacga tccaatttct tcaccaaggc
    29221 ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca tagagggttt ggtgagaatg
    29281 gatataacga gcgcagacac cgatagttgt tgatgggaca ccaccatttt tcagatgagc
    29341 tgcacctgca tctgttccgc ctttaccaca gtagtattgg tacttgatac cagcttcttc
    29401 agccgttgtc aaaaggaaat ccttcatccc tgggagaagc aagtgacctg gatcatagaa
    29461 acgaatcaag gttccatctc caatcttgcc ttgaccaccg tagacatcac ctgctggtga
    29521 gcaatcaact gcgaggaaga cttctgggtc aaacttggtt gtagaggtat gagcgccacg
    29581 cagaccaact tcttcttgga cgttagaacc cagatagagt tcattgccga gtttttgacc
    29641 tgataaagct tcagctagct cgcttaccat gaggacaccg tagcggttat cccaagcttt
    29701 tgagatgata tttttttcat tggctgtcaa aattgcagaa ctatctggta cgatggtatc
    29761 accaggacgg atgccaaaac tttctgcctc agccttgtcc gcaaaaccac catcaaaaac
    29821 gatatcggca atggctggca tggttggtcc cccctttcca cgagtcaaat gcggaggaac
    29881 agaacctgaa atcacaggaa tttcatgacc atcacgagtc aagagtttga aacgttggct
    29941 gctaaccacc atggggttcc agccaccgat ttctacgaca cggaaggtac catctggctt
    30001 gatttcgctg accataaaac caacttcgtc catatgagaa gcgaccaaga cgcgcggtgc
    30061 atccacagct tctgaatgtt tgataccaaa aataccaccc aagccatctg tcaccacttc
    30121 atccacatgc ggtgtcaact tttcacgaag ataagcacgg acaggcgctt catgacctga
    30181 gactgcagca agttctgtta cttctttaat ttttgaaaat aatgttgtca tttcagttcc
    30241 ttctttcttt catccatttt accacttttt ataggagaag gatagtggga aggtggattt
    30301 ctaagttagt atcttagtcc tgctctatct tagaaaagga tagtattctc ttgcatgtag
    30361 tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt tatttccaaa caaaaaaaca
    30421 atacaccatc aaagttgttt ggatttttca tgaaatttac agaaaatagt tgacttccct
    30481 ttcttctttc tttaaatata tagttggttg agtttggaat agtacgctgt agctgctgaa
    30541 acatttctag aaattaattt gactttccta atagagttgt tcatatctta tttcaattta
    30601 ctatagtaca aaactagaaa aggaaaaaat catgaccaaa acaattcttg ttacaggtgg
    30661 gactagctac attggctccc ataccgttaa agctctttta aatgctggct ataaggtgca
    30721 tgtcctcgat aatctctcca caggaaatcg agcggctgtg gatagtcgtg ctagctttaa
    30781 agaactggat gtttatgatg ctagcgcctt aaaggcttac ttagaagaaa atcagattga
    30841 tgctgtcctc cattgtgcag gtgaaattgt tgtgagcgaa agtattgaaa atccaagtaa
    30901 atacttcact gccaacgttg ctggtatgaa ccaagttctc aaagtcttat ctgaagttgg
    30961 cattcaaaaa atcatgttct cttcgactgc ttctctctat ggaaataact gtattgacaa
    31021 gccggtgact gaagataccc tgctcgaccc tgtcaaccct tatgcagaga caaaactgat
    31081 gggcgaacga atgatttact ggatggccaa tcgctacgac tggaaatatg ttattttccg
    31141 ttactttaat gttgctgggg ctgaaatgga tgcttcaaac ggtctgcgtg tgaaaaatcc
    31201 aactcacatc attccaaata tcaacaagac cgcattggga caaaatgata gcttaaaaat
    31261 atttggagat gactacgata cacgtgacgg ttcatgtatt cgagattaca tttatgtctt
    31321 ggaccttgca taggctcatg ttaaaggaat gaactaccta tttcaagaag acagttcttc
    31381 tcaaatcttt aacttaggaa ctaaaaaagg ctatactatc aaagaaatct ttaaaactgc
    31441 tgaagaatta ctaaattaaa aaatcccaca cgagattgtt gctcgacgtg ctagtgaccc
    31501 agccagtgtc ctagcagatg cttcaaaagc taaacaatat cttgattgga cggctagcta
    31561 ctccctcgaa gatattattt tatcagatta aggtaaaaac attttagaat aggataacaa
    31621 aggagaaagt cttgttgctc tctccttgtt gtatttatta aattgtcaga aattttattg
    31681 acagattaga aaaataatgt tacatttata tccgcagata tctttcgata ccaaatctac
    31741 atgaaaggac ggggtatgaa actttctcat tatttaattg gcttacttct actcctagtc
    31801 tttctctcta ttagcattgg gaccagtgat ttttcatggg gaaagctatt tgatttcgac
    31861 cagcagacct ggctcctctt tcaagagtcc cgtctcccaa gaactatcag tattctcctg
    31921 actgcctcta gtatgagtat ggcaggcctt ctcatgcaga ctattaccca aaatcagttt
    31981 gctgcaccga gtacagttgg aacgactgaa gccgccaaac tgggaatggt gctgagcctt
    32041 tttgtctttc catcggctag tctgacccaa aagatgctct tcgcttttgt ttcatccatc
    32101 gtattcaccc tcttcttcct agcctttatg accattttta ctgtaaagga aaggtggatg
    32161 ttgcctctga ttgggatcat ctatagcggg attatcggct cagtcacaga agttatcgcc
    32221 tatcgtttca atctggttca gagtatgact gcctggaccc agggctcctt ctccatgatt
    32281 cagacccatc agtatgagtg gctcttctta ggcctcatca tcctgataac cgtttggaaa
    32341 ttatcccaaa ccttcaccat catgaatcta gggaaagaaa ccagcgagag tttggggatt
    32401 tcctactccc tacttgaaaa actggccctc tttctggtgg cgctaacgac aagcgtcacc
    32461 atgattaccg tgggtggcct accatttctc ggagttatcg ttcccaatct tgttcgcaag
    32521 cgctatggag ataatctaag tcaaaccaaa ctcatggtcg cactggttgg tgccaatcta
    32581 gttctggctt gcgatatcct atcccgagtt ctgattaggc cctatgagtt gtctgtcagt
    32641 ctcttgctag gaatcatcgg tagtctcgtc tttatcctac ttctctggag agggggacga
    32701 aaagatgcag actaaaagca aacataccaa gctcttctgg attctcatta ttcttgccat
    32761 cggagcttgt cttctctact tttggcccat cactcacttg tcagcctttg cttggaagtt
    32821 gcgttcccaa aagatcatcg tttatctctt gatagccatc gcgactggga tttcgaccat
    32881 tagttttcaa accctgacgg aaaatcgctt cctgacgcct agtattttgg gaattgaatc
    32941 cttctacgtc ctactacaaa ccctactact ggtttttgaa agcaagtttc ttcaacttgg
    33001 caaatcccct atcttagaat tcctagtctt acttcttgtc cagtccctct tctttctcgc
    33061 cttacaaggt tacttgaaga cactgatgaa gcaagacctg gtcttcatcc tgctgatctg
    33121 tctagcgctc agaagtctct ttcgaaatat cagcaccttc cttcaagtcc taatggatcc
    33181 aaacgaatac gataaactgc aaaatagtct ttttgcctcc tttcaacatc tcaacacttc
    33241 catcctagcc atcggttctc tgatcatcct cgctttgaca atctttttct ttcgaaaagc
    33301 agtcgttcta gatgtcttgc acctgcaaag agaaacggct cagatattgg gactcgatgt
    33361 tgaaaaagaa cagaaagagc tcctctgggg aatcgtgctt ttgacctcaa cggccacttc
    33421 cttggtagga cctatggcct tcttcggctt tatgctggcc aacctcacct acctgattgt
    33481 caaagactat cagcacaagt tactctttat agtggccatt ctggttggat ttattagctt
    33541 aaccttgggg caagccttga ttgaacgagt ctttgcactg gaaattcgta tcagtatgat
    33601 cattgagagt gtgggtggct tcttattctt tatcttacta tataggaggg ctcgtcagtg
    33661 aaactggaaa acattgacaa atccattcaa aaacaggata ttttgcaagg catttcgctt
    33721 gaagtcagtc ctcaaaaact gacagccttt attggtccaa atggtgctgg aaaatcgact
    33781 ctcctctcca tcatgagcag actaaccaag aaagatcagg gagttctcag tatcaaagga
    33841 cgtgaaatcg agagctggaa ttcgcaagaa ctggcccaag aactaaccat cctaaaacag
    33901 aaaatcaatt accaagccaa attgactgtt gaagaactgg tcagttttgg acgttttccc
    33961 tacagccgag gtcgacttag atcagaagac tgggaaaaaa tccgagaaac tctgaactat
    34021 ttggaactga ccaacttaaa agaccgctac atcaatagcc tgtcaggggg gcaactccag
    34081 cgcgtcttta tcgctatggt actggcccag gatacggact ttatcttgct ggacgaacca
    34141 ctcaacaatc tcgatatcaa gcaaagcgtc agcatgatgc agattcttcg acgactggtg
    34201 gaggaactcg gcaagaccat tatcatcgtc ctccacgata tcaacatagc cagtcagtat
    34261 gcagatgaaa ttgtcgcctt caaggacggc caggtcttta gcaagggaag aaccgatcaa
    34321 atcatgcagg ctgacctgct cagtcaactt tatgagattc ccatcacgct agctgatatc
    34381 aatgacaaaa agatctgtat ctatagctag taacataaaa gctcaagtta gagaaccttc
    34441 agtctcttag tcaataagat caagagactc cctaaatcgt tatcacattt taaagaggag
    34501 aaattatgaa aacatccctt aaactttatt tcactgccct agtggccagc ttcttgctcc
    34561 tacttggtgc atgtagtaca aactcaagca ctagtcagac agagaccagt agctctgctc
    34621 caacagaggt aaccattaaa agttcactgg ccgaggtcaa actttccaaa gttcctgaaa
    34681 agattgtgac ctttgacctc ggcgctgcgg atactattcg cgctttagga tttgaaaaaa
    34741 atatcgtcgg aatgcctaca aaaactgttc cgacttatct aaaagaccta gtgggaactg
    34801 tcaaaaatgt tggttctatg aaagaacctg atttagaagc tatcgccgcc cttgagcctg
    34861 atttgattat cgcttcacca cgtacacaaa aattcgtaga caaattcaaa gaaatcgccc
    34921 caaccgttct cttccaagca agcaaggacg actactggac ttctaccaag gctaatatcg
    34981 aatccttagc aagtgccttc ggcgaaactg gtacacagaa agccaaggaa gaattggcca
    35041 agctagacaa gagcatccaa gaagtcgcta ctaaaaatga aagctctgac aaaaaagccc
    35101 ttgcgatcct ccttaatgaa ggaaaaatgg cagcctttgg tgccaaatct cgtttctctt
    35161 tcttgtacca aaccttgaaa ttcaaaccaa ctgatacaaa atttgaagac tcacgccacg
    35221 gacaagaagt cagctttgaa agtgtcaaag aaatcaaccc tgacatcctc tttgtcatca
    35281 accgtaccct tgccatcggt ggggacaact ctagcaacga cggtgtccta gaaaatgccc
    35341 ttatcgctga aacacctgct gctaaaaatg gtaagattat ccaactaaca ccagacctct
    35401 ggtatctaag cggaggcgga cttgaatcaa caaaactcat gattgaagac atacaaaaag
    35461 ctttgaaata agccacttca aactcaattt aatactcaat gaaaatcaaa gagcaaacta
    35521 ggaaactagc cgcaggttgc tcaaaacact gttttgaggt tgcagatgaa agctgacgcg
    35581 gtttgaagag attttcgaag agtataaaat tttgacattc taaaacgcat cctatcaagt
    35641 ttactcaaac ctgataggat gcgtttttat cattttgaaa tctatttacc ccacctcatt
    35701 ccccttcttg atttcgttta agggaaaagt ggtctggaac ggggtcctta cctgttttcg
    35761 accagggatg acaacgtaaa atccgagcca agcccatcaa taccccctta aaaccatgtt
    35821 tttcaatagc ctgaatcatg tagttggaac aagtcagctc aaagcgacaa gagggtggaa
    35881 agactggtga gataaaacgt tggtaaaagc gcacaggcgc tattaaaatt cgtttcatta
    35941 tttcttagtt acagccatgg tgtgtagttg gctgatttct tttttattaa gacgacggga
    36001 ttctcctgga cggagtcctg tcaagtctag gtgtccgaaa cgagtccgag acaacttatc
    36061 tacttggaga ccaacagctt caaacatctt tttaacctga tggttacgcc cttcatggat
    36121 ggtcaactgc accacagagc gatttttgac tgggtccact ttgagaattt cataaacagc
    36181 tggcttggtt ttcttaccat caatctcaag accacgggtc aaggggcgga gattgtcctt
    36241 attggccaca cctttaacac gcgcgacata aaccttgtca atctcattac gagggtgaat
    36301 catctcgtct gtaaagtccc catcattggt caaaatcaag acacctgatg tatcccagtc
    36361 caaacgaccc acagggtaaa tacgctcttt gacattgggc aagaggtcga caaccgtctt
    36421 gcgaccctta tcatctgtca cactggaaat cacaccgcgt ggtttgttaa gcagatagta
    36481 gaccttttct tcgttgtaga taggttgacc ttcaacttcg accttgtcgc ctgacttgat
    36541 agtggttgct agttcacgca ccacttggcc gttaaccgtc accaagcctt gcttaatcag
    36601 ctcttctgct tttctcctac tggccacacc tgcgtgggca atatacttat tgattctcat
    36661 cttcttctat cctttcacca aataattggc tttcttgggc ttgaatctca agctcatcaa
    36721 tcactggtaa ttcttctaaa tggtttatcc ccatgtaatc taggaaataa tccgtagtca
    36781 catagaggtt ggggcgcccc aatacttcct ttttcccgtc ttcctttatc aggtcaaaag
    36841 cctgcaactt tgccaaggct ccactcgagt taactccacg gatggcatca atttctatcc
    36901 gcgtaatcgg ctgtttgtag gcaataatgg acaaggtctc aagggcagcc cgagacaagc
    36961 tctggttgat aggcgcctta gagtattcct tcaaaatctc tgcaaattga ggcttggtca
    37021 ccaatctata agcaccactt gtctcaatca aagccaaact ggaatctggg tccttttcat
    37081 acttctgggc taattttcct aaactttgct ggatgcctgt cggtggcaga gagaggagtt
    37141 cagctaactg gcggacccga atcccatctt cacccgctac aaacaagagc gcttctattt
    37201 ttgctaaagt actcatcttt cctctctatc aagtctagct ttgaggcact tgactttctt
    37261 ccttcttttc catgagatag atatctccaa aactctcctc ttgcacgagg atcagctcct
    37321 gggttttgat taactctagg gttgccaaaa agagggtgat gacctcttgg acattctggg
    37381 cttccttgaa caaatcctgc aagcgcaatt gatctcgtcc aatcaaggac tctttcacga
    37441 taatcatcat gtcctcaatc ttatactcat cccgcaagat cgtcgtgtga ttttgtgcaa
    37501 actcctcttt tttcttggct aggatatttg aaaaagccaa aaagaggtca atggtcgtct
    37561 tgtcatgcac aagctccgca tcttcgtaaa tcaactctgt cggcgctttg gaataatact
    37621 gggcccgttc ttggtgcttg gcttccaagt gctcacccaa gagcttgaac ttgcgatatt
    37681 cttcgatttg agagaggagg tcctgctcca ggtcatcccc caagtctgtc acttctgcta
    37741 ccttcggaag gagtttacga ctcttaatca gcatgagctg actagccatg accatgtact
    37801 cacccgtcac ttccagacgc atggcctgca gggttgagac ataggctaga tactgttcga
    37861 tgacttccgt aatgggcaca tcgtagatat ccatctggta cttagaaacc agatgcaaga
    37921 gcaagtccag gggtccttca aaatctttta atttaatatc cattatctat atttttctaa
    37981 ggtcaggact gtttttaatc ctaatttttt tgcaatttcg tacaaatcga ccttgttttc
    38041 tatttgtctt agaataaact gttcacgtaa gacttgagct gataaggatg gaaaaccttt
    38101 ctccttgaca aaagattcta actgacgaaa ggcccactga cgagaatagg gtttctctcc
    38161 tctttcaaaa agataggtct gccccatcaa gggttccaat tctgaaagca aggccgtggg
    38221 aatggtgaca atcctctgtt gggaagcctt gctgattcgc agcacctgaa aatccagatt
    38281 gatgtccgca accttgatgg ctaaaatctc actgggcaag agccccattt ctaggattaa
    38341 gagcgctagc aagcggccct ctggatggtc gctttcctgc caaaaagagt ctaggtatag
    38401 aatctctggc ttttccgtct tcttttcagc ttgtttggct aattccaagc ggtaaaagct
    38461 gtccacctct cctttttgat agagaaagta tagaaattgg ttacaggccg aaatctttcg
    38521 cttctgggcg ctgattttta gattggctag ctgggcttgg taaatcttga gactggtctc
    38581 agaaatccgc tcacctacca tgtctaaaaa ttgctccaaa tcatacttat aggactgctt
    38641 ggaattgaca gacaagccct gcttttcctc taaaaaggct gaaatcctgt ctctcattgg
    38701 catcgaatct catattcctt actaaagtca tgcaagaggg cattaacggc cttgaggatg
    38761 gacttgcgcg taataatccc ttggaaaata ccctctgcat ccacgaccgg taagaaggac
    38821 tcatctacta gcttgtgcaa gacctccgta atggtgaaat caggcgaaac aaccgctacg
    38881 tccgtttttg tcatatgaac gatatccgta tccgccatga tttcttggct caagtcatgc
    38941 tccatctgat aagccataat atctctgagt ccaatcgtcc caacaaactg tttttcatct
    39001 gtcacaacgg gaacacgggt ataggtcatc tgactgagca agagggtcgc atgatccgca
    39061 ttgtgggtat caatcaacac agctagattt ttagcagggg tcaaaaaagt ttcctcctgc
    39121 cccaacaaga aagtctcaaa ctccttggca atcatcggct aaactccttg gacaaacctg
    39181 gatacacctc gtgatctcgt gtcaaaaagt ccactttgaa gtaactatca tcaatctcca
    39241 cacgagcata gagacattct ctgatggtac ctcgtggttg actgatagaa cctggattta
    39301 gaaagaggat cttgccttcc aaccaagcac ttggcacatg caagtgacca tagaggcaga
    39361 tagcggcctc ttcctcctga gcccagtagt ccaacttttg aaagttgaaa ttgatgtcaa
    39421 acaagtgacc atgagtttgg ataatcttgg tcgaaccaag ctcagtcacc agacgttctg
    39481 ggtagccggc gtagaagtcc atgttccctt taacaacgcg gatgccctcc caaagtggag
    39541 aatccggacg tagttcagaa tcgccgttat gaaaaacggc atcgactttg cccacatagc
    39601 gatcacggac ttcttccaca atcaagctat cgccatggga atcgctcatt acaatgatgg
    39661 tttgctttgc catgatggaa atacctccaa aagtttctta acggctaagg cacggtgaga
    39721 ttgactattt ttttcttcta gggttaattc agctgatgac tcacctgtct ctcctacaag
    39781 gaagagggga tcatagccaa agccattttc acccttaggt tcaaagttaa tataacctga
    39841 ccagtctgct tcaacaacta aactttcctt atttgggctg gctacgacta gggttgtgtg
    39901 gaactgagcc gagcggtcct tgagttcaaa gaccatggcc aattcgtgca agagtttggc
    39961 attattttca cggtcagttg ctcccacacc tgcgaaacga gctgaccaga cgcctggtaa
    40021 gccaccaagg acatcgactt tgagaccaga atcatctgcc aaaaccatct tacccgttaa
    40081 ttgagaaatg gtttctgcct taaggcgggc attttcttca aaggtcatac ctgtttctgc
    40141 tacttcaggc aggtcagggt agtcattaag attttccaca tcgtagccta acttatcaaa
    40201 gatagctcgg aattccttgg ttttgccttc gttacgagtc gcaatcaaca aggtttctct
    40261 gaccttgctt gtctcaaaga aatcatgaac attgacccct tctttaggca attctacata
    40321 caagagttgc ccattttcaa ccaagagcag ggccccatga cgttgaataa cttccaagtt
    40381 tcgtcccatt tcttgattaa gcatatctac cacaaaggag aacaaacggt aggtagaacc
    40441 atagcgtaaa acagtaactg aaagcgggaa accatactct tcatctccaa atattttgga
    40501 gaattcaaac agaggaaaat ctgtcttata gtcgctcaaa ctgttaacgc caccaaaaat
    40561 actataagac ccaacatacc agtcctggtc atccttatat tcataaattt tatttgtcat
    40621 aattctacat gctccacatg aatctctttt tccagccatt cttcaccaat ttgtgcaaaa
    40681 ctttggctac tggctgttgt gtaaaaacgg tgatggagtg gtccagcatc gcgaccacga
    40741 ttgatttcaa aataattgag taagactgag atatcccgta cgcactctgc cccactatcg
    40801 atgagctgaa cctttggccc catcacattt tggataatag ggcgaaggag tggataatga
    40861 gtacagccca aaatcaggct atccaccttt ccaaccaagg gacgcagggt ttcatagacc
    40921 accttcttgg taacactggt tgacagggca cctgactcaa ccaagggagc aaacttggga
    40981 caggccaagc tctccacctg taagtcggga tccagatcat ggattttctg acggtatatg
    41041 tctgattgta ccgtcatggg cgttccaatc actccgattt tcccaccttg actggacttg
    41101 atggctgccg aagctcctgg caaaattaca cccaagacag gaatatctag ttgagccttg
    41161 atttcttccc agacgaccgc agtcgcagtg ttacaagcaa tgacaatcat tttgacatcc
    41221 ttggtcaaga gaaagttgac cagctgccaa gtatattcac gaatttgctc agcaggacgg
    41281 gggccatagg gcgcccgcgc cgaatctcca atatagacga tttcttcatg gggaagctgg
    41341 cgcatgagct cgcgcacaac ggtcaagccc ccgacacccg aatccaaaaa accaattggt
    41401 cgattatcca tacagtcttc tttctagtct tttttctgat tgatcgttca ttatgattcc
    41461 ttgtactgaa cgaactgacc gacagcttga tagaatgtca actacctctc tcttaatcat
    41521 aatcaaatat caatagaatg caaggaaaaa gtctcatccc taaaccataa ccaacatagt
    41581 gagaagtctg gaactttcat cccagacttt tccttattta ttttttcttt ttattgttag
    41641 caagggctgc cttttgttgg cggatgattt ggtggtaaac ttgttgtacc ttagcttcgc
    41701 ttggtttttg accatttgca ctcaaaagag tacgaactgc ttcagcattc aaacgtgggt
    41761 tgtcagcaaa ttctttttcg atttgcttac gaaccaagta cattcctcca agagctcctc
    41821 ctagaaaagc tagcacaatc aatacaattg ctaaaagtaa atccatcatt tttctcctgt
    41881 ttctttttga gtccctttca ttataccaca aactagccac cctgaccagt ttgttctaca
    41941 ctttcaagga tggaatagac agcaaaaaga accctgcttt tctgaaaaag aggtctcctt
    42001 gctaagttta tactcaatga aaatcaaaga gcaaactagg aagctagcca caggttgctc
    42061 aaagcaccgc tttgaggttg cagataaaac tgacacggtt tgaagagatt ttcgaagagt
    42121 attaatttac ataaatagcc agtgtttgat agggtttgag tagaattttc tcagacactt
    42181 ctgcatcttc atagtttgaa atcaaaatct gtccattttg gtagactgct ggcaagtcga
    42241 tttccacttc tttagcataa aagttattga gcactagtaa cttttgatcc tcaaactggc
    42301 gttcaaaagc gtagacttgt ttgctatctt caaaggctgg tttgtaactt ccttctgaaa
    42361 tgattggcat ttccttacgc agtcgaatca agtcttgata gaaggtaaaa atcggaccct
    42421 ggatttcatt ttctacattg atgtctttat aggatttacc agctttcaac caaggagtgc
    42481 ctgttgaaaa tcctgcattt tccgaagcat cccactgcat gggaatgcgt gaattatcac
    42541 gcgacttagc ttgaataatc tggaaggctt cttgctgact ctttccttct tctaagagca
    42601 tctgataggc attaagcgat tcgacatcca cataatcagc catagaatca tagtctgggt
    42661 caatcatccc gatttcctca cccatgtaga tataaggtgt cccacgtgac aggtgaatgc
    42721 tggccgctag catggtggct ccttccttgc ggaagttttg aatatcgaca aaacggttca
    42781 aggcacgtgg ttgatcgtga ttgttccaaa agagggcact ccaaccgtct ttatcactca
    42841 tttccttacc ccaactatgg taaagactct tcaactcttc aaaatcaaag ggagccaagg
    42901 tccacttttg tccatcctta tagtccacct tgaggtgatg aaaattaaag gtcatggata
    42961 attcctgacg atcaggcgac gaatagagga cacagttttc catggtggta gaagacattt
    43021 ccccaactgt cataaagcta tcgtcagatc caaaagtggc ttggttcatc atacgcaaat
    43081 agttatgaac gatgggtttg tctgtataag ctggcttccc ttcattttca ggacagtcca
    43141 ctgaaacctc gtccttaccg atcaaattga tcacatcaaa tcggaaacct ttgacaccct
    43201 tgtcgcgcca gaaattaaca accttgaaaa gctccttacg gacattggaa ttgcgccagt
    43261 taaggtcagc ctgggtctca tcaaataggt gaagatagta tttcccagta tccccgaaag
    43321 gcgcccatgc agaaccacca aacttagact gccaatctgt tggttggtct tggatgaaga
    43381 aaaagtcttg ataatactta tcaccagcta gggctttctg aaaccattca tgctctgtcg
    43441 aacaatgatt aagtaccatg tccagcataa agtcaatctt gtgctcttta ccgacacaca
    43501 ccatttcctc aaaatcagcc atatcaccaa aaagaggatc cactgccata taatctgaaa
    43561 tatcgtaacc attatcccgt tgagggcttg gatagaatgg attgagccag accatatcca
    43621 cgcctagttt ggctaaatag ggaatttttt cgataatccc acggaaatcc ccaataccgt
    43681 tttcagtggt gtctttgtaa gattttggat agatttgata gactactttt cctttatcaa
    43741 gtgtcatctg tttctccttt tctgataaaa gggaggaagc agtcttccgt ccctatttgt
    43801 gctatttcaa ttatactcaa tgaaaatcaa agagcaaact aggaagctag cc
//
LOCUS       DAVMMR010000010        42313 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMMR010000010 DAVMMR010000000
VERSION     DAVMMR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.010"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="VYS74_000534"
     CDS             complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="VYS74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV3788615.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
     gene            complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="VYS74_000535"
     CDS             complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="VYS74_000535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV3788616.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="VYS74_000536"
     CDS             complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="VYS74_000536"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV3788617.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIINTVLYIEKIDR"
     gene            complement(3790..5058)
                     /locus_tag="VYS74_000537"
     CDS             complement(3790..5058)
                     /locus_tag="VYS74_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV3788618.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5178..5546)
                     /locus_tag="VYS74_000538"
     CDS             complement(5178..5546)
                     /locus_tag="VYS74_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV3788619.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5539..5805)
                     /locus_tag="VYS74_000539"
     CDS             complement(5539..5805)
                     /locus_tag="VYS74_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV3788620.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="VYS74_000540"
     CDS             complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="VYS74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV3788621.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9373..9942)
                     /locus_tag="VYS74_000541"
     CDS             complement(9373..9942)
                     /locus_tag="VYS74_000541"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV3788622.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10014..11129)
                     /gene="ychF"
                     /locus_tag="VYS74_000542"
     CDS             complement(10014..11129)
                     /gene="ychF"
                     /locus_tag="VYS74_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV3788623.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11213..11407)
                     /locus_tag="VYS74_000543"
     CDS             complement(11213..11407)
                     /locus_tag="VYS74_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV3788624.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11472..12608)
                     /gene="dnaN"
                     /locus_tag="VYS74_000544"
     CDS             complement(11472..12608)
                     /gene="dnaN"
                     /locus_tag="VYS74_000544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV3788625.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12767..14128)
                     /gene="dnaA"
                     /locus_tag="VYS74_000545"
     CDS             complement(12767..14128)
                     /gene="dnaA"
                     /locus_tag="VYS74_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV3788626.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14341..15099)
                     /locus_tag="VYS74_000546"
     CDS             complement(14341..15099)
                     /locus_tag="VYS74_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV3788627.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15157..16338)
                     /locus_tag="VYS74_000547"
     CDS             complement(15157..16338)
                     /locus_tag="VYS74_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3788628.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16521..17000
                     /gene="rlmH"
                     /locus_tag="VYS74_000548"
     CDS             16521..17000
                     /gene="rlmH"
                     /locus_tag="VYS74_000548"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV3788629.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17044..17117
                     /locus_tag="VYS74_000549"
     tRNA            17044..17117
                     /locus_tag="VYS74_000549"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17078..17080,aa:Arg,seq:tct)
     gene            17283..17408
                     /gene="comC"
                     /locus_tag="VYS74_000550"
     CDS             17283..17408
                     /gene="comC"
                     /locus_tag="VYS74_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV3788630.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17429..18754
                     /locus_tag="VYS74_000551"
     CDS             17429..18754
                     /locus_tag="VYS74_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3788631.1"
                     /translation="MDLFGFGTVIVHLLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIAIFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFS
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18751..19503
                     /locus_tag="VYS74_000552"
     CDS             18751..19503
                     /locus_tag="VYS74_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788632.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQFIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19555..19626
                     /locus_tag="VYS74_000553"
     tRNA            19555..19626
                     /locus_tag="VYS74_000553"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19588..19590,aa:Glu,seq:ttc)
     gene            19632..19705
                     /locus_tag="VYS74_000554"
     tRNA            19632..19705
                     /locus_tag="VYS74_000554"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19666..19668,aa:Asn,seq:gtt)
     gene            complement(19746..20288)
                     /locus_tag="VYS74_000555"
     CDS             complement(19746..20288)
                     /locus_tag="VYS74_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV3788633.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20467..21234
                     /locus_tag="VYS74_000556"
     CDS             20467..21234
                     /locus_tag="VYS74_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV3788634.1"
                     /translation="MIGISLIPALYNIIFLSSMWEPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21238..21657
                     /locus_tag="VYS74_000557"
                     /pseudo
     CDS             21238..21657
                     /locus_tag="VYS74_000557"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21685..21999
                     /locus_tag="VYS74_000558"
     CDS             21685..21999
                     /locus_tag="VYS74_000558"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV3788635.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(22100..24652)
                     /locus_tag="VYS74_000559"
     CDS             complement(22100..24652)
                     /locus_tag="VYS74_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV3788636.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLNRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24714..26336)
                     /locus_tag="VYS74_000560"
     CDS             complement(24714..26336)
                     /locus_tag="VYS74_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3788637.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26542..27567
                     /gene="trpS"
                     /locus_tag="VYS74_000561"
     CDS             26542..27567
                     /gene="trpS"
                     /locus_tag="VYS74_000561"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV3788638.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27720..29198
                     /gene="guaB"
                     /locus_tag="VYS74_000562"
     CDS             27720..29198
                     /gene="guaB"
                     /locus_tag="VYS74_000562"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV3788639.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29249..30346)
                     /gene="recF"
                     /locus_tag="VYS74_000563"
     CDS             complement(29249..30346)
                     /gene="recF"
                     /locus_tag="VYS74_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV3788640.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(30349..30717)
                     /gene="yaaA"
                     /locus_tag="VYS74_000564"
     CDS             complement(30349..30717)
                     /gene="yaaA"
                     /locus_tag="VYS74_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV3788641.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30876..32126
                     /locus_tag="VYS74_000565"
     CDS             30876..32126
                     /locus_tag="VYS74_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3788642.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNTLFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32123..33406
                     /locus_tag="VYS74_000566"
     CDS             32123..33406
                     /locus_tag="VYS74_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3788643.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTLAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33448..34269
                     /locus_tag="VYS74_000567"
     CDS             33448..34269
                     /locus_tag="VYS74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3788644.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34280..34825
                     /gene="pgsA"
                     /locus_tag="VYS74_000568"
     CDS             34280..34825
                     /gene="pgsA"
                     /locus_tag="VYS74_000568"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV3788645.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIVLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34822..35649
                     /locus_tag="VYS74_000569"
     CDS             34822..35649
                     /locus_tag="VYS74_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3788646.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRKIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35634..36473
                     /locus_tag="VYS74_000570"
     CDS             35634..36473
                     /locus_tag="VYS74_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3788647.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36466..37260
                     /locus_tag="VYS74_000571"
     CDS             36466..37260
                     /locus_tag="VYS74_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3788648.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37319..38137
                     /gene="mreC"
                     /locus_tag="VYS74_000572"
     CDS             37319..38137
                     /gene="mreC"
                     /locus_tag="VYS74_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV3788649.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38137..38631
                     /gene="mreD"
                     /locus_tag="VYS74_000573"
     CDS             38137..38631
                     /gene="mreD"
                     /locus_tag="VYS74_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV3788650.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38725..39903
                     /locus_tag="VYS74_000574"
     CDS             38725..39903
                     /locus_tag="VYS74_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV3788651.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40127..40906
                     /gene="rpsB"
                     /locus_tag="VYS74_000575"
     CDS             40127..40906
                     /gene="rpsB"
                     /locus_tag="VYS74_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV3788652.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            40985..42025
                     /locus_tag="VYS74_000576"
     CDS             40985..42025
                     /locus_tag="VYS74_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV3788653.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDKLAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT        13268 a         7290 c         7800 g        13955 t
ORIGIN      
        1 tttcgtcatt catttttgac tttacttcat cataggatag tgcatgagat tcctcttcta
       61 ctgcttcagg catctttcct gtttcgtaaa gagctttaat ttgtgtacta tccaatgttt
      121 cgtatttcaa taatgcttct gcaattaact tgtgagtttc acgatttgac tgaataattt
      181 cagcagcttt atttcgtgcc tcatttaata atgaacgaac ctcttcatca atttcataag
      241 ctgtttgttc tgaaattgat ttttgaggat tctgtgcacc aaacatagca tggtttcctt
      301 catattgtac tgggccaagt ttttcactca taccgtactc tgtaaccatt gcacgtgcca
      361 tttgtgtcgc ttgttcaaag tcgtttgaag ctcctgtagt ttggacatta aagataattt
      421 cttcagctac acgtccaccc attaagccag ccaattgctc tttcatatct tctttagata
      481 gaagcatttg atcctcttta ggaagtgcaa tcatgtatcc gcctgcacgg ccgcgtggta
      541 caattgtaac cttatggaca acgcgagcat tcgacaagac tagaccaaca atggtatgtc
      601 ctgcctcatg gtaagcaacc aattctcgtt ctttttgtga aactgtctta tctttcttag
      661 aaggtccagc aataactcta tcttctgctt catcaatatc tgaagcatca attatcgatt
      721 tattgcgacg agcagcaact aaagctgctt cattcaagac attctctaaa tcagcaccaa
      781 caaagcctgg agtttgttga gccactaatt tcaaatcaac atcttctgct aaaggcttgt
      841 tcttagcgtg aactttcaag attgcttcac gacctttaac atcaggacgg ccaaccaata
      901 cttttctatc aaaacgtcct ggacgcaaaa gggcaggatc aagtacatct gaacggtttg
      961 tcgcagcgat gacgataatc ccttcatttc cctcaaaacc atccatctca atcaaaagtt
     1021 ggttcaaggt ttgttcacgt tcgtcattac ctccgccgag accgactcca cgttgacgtc
     1081 caacagcatc aatttcatcg ataaagatga tagctggtgc tgcttttttg gcatcctcaa
     1141 aaagagagcg aacacgacta gctccaactc cgacaaacat ttctacaaag tcagaacctg
     1201 agatactaaa gaatggaaca cctgcttctc cagcgactgc cttagcaagc aaagttttac
     1261 ctgtccccgg aggtccctcc aaaagaacac ctgctggaat acgggctcca agttttgtga
     1321 atcgttttgg atcttttaag aactcaacaa cttcaactag ttcttgtttt tcttcttcag
     1381 ctccagcaac atctgaaaat cttactttaa tatcttcttt atttgctgct ttagccttac
     1441 tacgtccaaa actcattgga ttacggccat tgcctcctcc catatttccc atcatagaga
     1501 ataggaagaa gaatagaatt ccaaatggca caatggatac gagtagatta atccatatac
     1561 cacttgaact ttcatgctta acagttactt ctgctttatg gtcagtagca agtttttgca
     1621 attctgatac ggtagtatct gcaggaagaa tagtgctggt aaatttctct accttagtaa
     1681 cagatggcgt gaaaaactga atacctgttc cttctttact tgttttagga tttttataga
     1741 caccagaaac ttcgataaca ctaccatttg gttggtaagt taattctttt acattaccat
     1801 cggtaatttc ttgtaccaac tcagtatagt tgatttgctg acttcctcct gagttattcc
     1861 cagaatagaa atactggaat cctgtcacaa gaaaaaagat aaataataac catagaaaag
     1921 gatttttaat taaaccatta ttttgttttt tcattaaaga ttattctttc taatttgaat
     1981 acacttcctc tttcaatact ccaatataag gaagattacg ataattttct ttgtagtcta
     2041 aaccataacc tactacaaac tcatttggga tagtaaagca ggtatagtct gcctcaattt
     2101 ctacaacacg tccttctggt ttatccaaca aggttgcaat tttaacagaa gctgcttctc
     2161 tttctttaaa catatctcgc aaattcttca aagtttgacc tgtatcaatg atatcttcta
     2221 caaatagaac atgtcttcct ttgatatctt gagtcacatc ttgtttaata ttgataacac
     2281 cactacttgc tgttccacca tggtagctag aaaccatcat gaagtccatt tcaatatgtg
     2341 tatcaatatg tttgaccaat tcagccataa aaggaataga tccttttaaa atcccaacta
     2401 agattggatt ttttcctgca tagtctttag ttaattgagc acctagtttt ttagctgctt
     2461 ctgtaatttc atcgtgtgaa acgaggactt ttttaatatc gttttctaac attttttacc
     2521 tatctatttt ttctatataa agtacagtgt taattatatc atttttcgtt tttttactca
     2581 aattattggt cgcaattccc aaaattgaga caatttcacc aaattgctca ataataagag
     2641 cagagtttct cttttccata gggattttca aatcaataaa taaacgtctg agtttttttc
     2701 tatgcccatt ttgaatcaaa acatctcctg tttttcgatg acgaatgtgt atggatgttt
     2761 cacgtgaaac aggtatttgt tgaattaatt caccttctaa tggaagtcca aaggaaaata
     2821 aatatccttg ataagctacc tgattttgat agtgtaacac aagttcatct tccttttcat
     2881 cagcctgcgg actgatttta caaatctgaa actgttggta ctcttttatc aattcatagc
     2941 catttttaat cggatgacga tactggcttt tagattttaa aatctgctga acttcagcaa
     3001 actgagcttt tgtaagattc aaatctggaa aacgattcag ataagtttga agtaaaactc
     3061 tttgtgtaga ctcagagtaa gaaaataact gctgtaaatc ttccacatta atattgttag
     3121 ataattcagc tattgccaaa tcataatcta aaatttcatt gccaatgcct aagattgcat
     3181 ccctaaatcg aggattttct ttttccaatt ctggtaagta agagtttcga atacgatttc
     3241 gaaaataatg attctcctga tttgatgtat cttcaaagtg aaaaattgat ggaaagtctt
     3301 ttttctgaaa atgcaagaag ggacgaatga tttctatctc tccgactact tgcttctcct
     3361 taattcctga tagatagcgc aagcgagttc ctcgaatcaa gcgcatcaaa atcgtttcca
     3421 cctgatcatc agcatggtgg gcagtgacta aagctgtcgc acctgtcttt ttcataacct
     3481 cttgaaaaaa atcataacga aaatttcgtg cacgcgcttc tgaaaattct cctgaaaaat
     3541 tgctgatata aataggaagc tctgcttcag cagccaactt ccttaattcc ttttcttccc
     3601 aatctgattc aattctctgc ttatgattca catgagctag aatcaattca atctctaact
     3661 ctttttgata agtagacaat accttaaata gaaacatgga atctaatcca ccagaaagag
     3721 ctagaaccgc cttagcatgc tttttgaaat atcccttctt gagaaaatga tttaaaaaat
     3781 ctggttccct cattttagaa cctcataaac atccttggct atcttagaaa tcgtatcata
     3841 atcagaattc ttagtgaaaa tagaaagaat aaatggagaa tctgcataga caacacccgt
     3901 atcatgctta aattcatccg catctccaat tttatgagct actttaacag aaacaccttt
     3961 ggcaattcgc tgactatcaa aatctgtttt agtcaaagac tctagcacaa atccattttg
     4021 attataaata gcttccataa acttcccggc catcttagaa gaaatcaatt tttcttttgg
     4081 atcccaatca tctcccataa tggcagacat cttggatttg aatgtggcat cagattggtt
     4141 tgaaatgtaa tatcccaata gattatgagc tacattatca gattcttttg atactttcgt
     4201 aattaaatcc tttaaagaat attctttatt atcttctttt ttaggaagac taccacttcc
     4261 ctctggttta taagaacctg gaaaatcatt gactgcagat acgtatttta cagtcgtatc
     4321 taactgataa agaccctcat ttattttttc ttgcgtataa tagagatagg agagtttcaa
     4381 aacgctggct gcatacatct tttcatcttg attgatacca gcttcttttc cagtagtcag
     4441 ttgcttaaca taaatagaga aagaatcttt ctgatatttt tcagataaca tttcttgaac
     4501 tttactcatc cgattatctt cttcagaagt tgattcttta gctacccatc cagcctgatc
     4561 aatatgtaga aattctcttc cttctacaaa catggtcttg tcgattgata cttgcgaata
     4621 agctgataag gatgatttca cttcttttaa atcataagga ctattgtaca gtttaaagtc
     4681 agattctaac catgcttttt ttattgttgg agttacctct gattgatcat ataaaaatcg
     4741 tttgtccgca gctataaatt gatgatttga tagcttaaat actggaattc cttgtttatt
     4801 taagcgccac tcggttattt gaaaacttgt tttgggagtc aatttcccag attccactac
     4861 taaatcttca ttcgcataaa caggaacctc tccataaacc atgggagaac ttaatttttc
     4921 tctaaaataa atactaaagt cagattgtga aaggtaataa atttctttcg aagtatagac
     4981 gacttctttt tctgtgctaa cgacttttga aatggtcaaa aaacttggta gcaacaaaat
     5041 aattaagaat ttacgcattc ttcctttcct tttcttcttg taaacgcaat aattgatttt
     5101 tatcagccaa ttcttccagc ttttcatctg acaaactcaa aaattgctct attacgtcta
     5161 ataaattttc cattttatca cctttgaagc aagtcaggaa tcgtataaac tttttccctc
     5221 gacttagaat aatagtactt cgctcgtgta tatttagcag cataatcttc atctttcaac
     5281 ttggtagcaa atgctgtctc cttatccttt tcatcactca aagtttgata ctgagtttgc
     5341 aagtctgcta attgctgacg tctttggagt aattgctgat aactctgcgc taaattaaaa
     5401 gttggcaaga taaataatag cataatcaaa atcaataccc ctcccataaa acgattccgt
     5461 ttttgtcgtt ctttcatcag gtagcgacga cgttggtatt cattttgaat aaaagaatta
     5521 ttcaattgta caatattttt agacattttc ttctacccgt gtttcactga taatttcata
     5581 cattcctgct gcatcttctt tttttgtact atctttcatc tctagtactt ttacaagcag
     5641 caacttattg ccaaagcgaa tttcaacttg gtcattaact ttcaagtccg ttgaactttt
     5701 ggccaagatt ccattaacct tgattctacc tttatctgct acttcctttg cgactgtacg
     5761 acgcttgata attcgcgata cttttaaata tttatctaat ctcatcctta ttacctcaat
     5821 ttattattgt accattttta tctattttat agaagaaaaa tatcaaatgg aattttcttc
     5881 cttaaactct tttatctcta ataaactttc tccaaaaatc agcagacctt ctaaaatttc
     5941 ataatctttc ttattttgga catcaaatac aagctccatt aatcccttat tctcagcgat
     6001 gcctgctttt aagttcgtta cggataaagc tttaaaataa tcttgagcta aaaacagccg
     6061 ttgagtgact ttttcaaatt gaattgtaat tttattatct tttctttcca cacgttgaac
     6121 aaagaccttg tccaagtatg atttaaccaa accaatctct aaaagatagg ctactacatc
     6181 tgggtattct ccaaaacggt ctatcaactc ctcttgtaac tcttcataat tgacacggtt
     6241 gtcaatttga cgaattttct tgtaaatttc aatcttatgt cgttgatcag aaatataagt
     6301 atcaggaaga taggcatcaa tttgcaaaat caactcagca ttccctttgg ttcttgtgtt
     6361 agcgttagca ttaccgtttc gtttagcaat agcttcctct aataactgcg aatacaattc
     6421 aaaaccaaca gaatcaatga aaccagactg ggattttcct aaaagatttc ctgctccacg
     6481 aatcgaaaga tctcgcattg caatcttaaa gccagagccc aattctgtaa atcctttaat
     6541 cgcttctaat ctcttttcag agacttcact gattgatttt tctggacgat acatgagata
     6601 agcataagca atacgattac tacgaccgac tcttcctctt aactgatata aggttgacaa
     6661 gcccatatgg tccgcatttt caataaataa agtattagca tttggaatgt ccacccctgt
     6721 ctcaataata gtagtcgtca ccaagatatc gtattgtccc tcaataaagt ctaatagagt
     6781 attttccaac tggacttcac tcattcgacc atgaacatat ccaatcgaag cctccggaat
     6841 caactcctgt aattctgaaa ccttctgaac aattgtgtca actttgttgt aaagataata
     6901 aacttgacct ccacgctcca tttcacgcaa gacagcatca cgaatgacac tatcattctt
     6961 ttccaaaaca taggtctgaa caggatagcg attagtcggc ggagtttcaa taacagataa
     7021 atctctgatt cccagcatag acatatggag ggtacgaggg attggcgtag cggtcaaggt
     7081 taggacatcc acttgtttct tcagttcttt caaagtttcc ttatgcttga caccaaatcg
     7141 ctgttcctca tcaataatca tcaagcccaa atcagcaaac acaacatctt ttgacaaaac
     7201 acgatgtgtt ccaatcaaaa tatcgacttg accgtttttc aatttttcaa gtgttgcagt
     7261 ctgctctttt ttacttctaa agcgactcaa cacatcaaca ttaactgcaa aattttggaa
     7321 tcgttcctta aaattcgtat agtgctgttg cgctaaaacc gtcgtcggaa ctagaatgac
     7381 aacctgtttg tgatcattga ctgctttaaa ggctgcacgc atagcaactt cagtctttcc
     7441 aaaaccaaca tccccaacta aaagtcgatc cattggctga gaagcctgca tatccctctt
     7501 gatttcctca atactacgaa gttgatcatc cgtttcaaca taagggaaag catcatcaaa
     7561 ggcatcttga tcatcatcat cagctgagaa agcaaaaccc ttcaactgac tacgttcaga
     7621 gtagagtttg attaaatcat cagctatatc ctctacctgg ttcttaacct tttgcttggc
     7681 ctttttaaaa tgaccgtcat ttaatttatt gagttttgga gctttaccat cacttgaaat
     7741 atatttggac agtagatgaa tctgttccac ggggatagaa atttgatcac cattttggta
     7801 ttggacactg acataatcgc gatgaattcc cttgatttca atggtttcaa ttcctagata
     7861 ttgaccaatc ccatggatat gatggacaac atagtcccct ttttcaagtt cattgtaatc
     7921 ttttaatctc tctgcatttg aaacatgttg tcttcgaaaa cgacgcttta atttcttttg
     7981 aaaaatctca tgttcagtta tcaataaaat cttttcatct acaaaatgaa aaccatgtct
     8041 gagattaccc tctattaagt ttacagattc tttacagata cttgtcttat ctctagaatc
     8101 caatttaatc tgatattcct ctaacatatc ctccaatgtt ttacttccca ttgaattgct
     8161 agactgcaga ataatggtgt aatccatttt tttatatcgt tcaatttctt cttttagaaa
     8221 agaaaactga ttgaaaaatt cctgcatagg atattgatta aattgataaa ttttgtcaaa
     8281 tttgagattt cctaaaccct tttgaagatt agagaaaaag gtcactggac tttgtttttt
     8341 atagatttgt tcaatatcag aaaaatactg catatcagaa aatgctttac tattctgtaa
     8401 ttcttctgta aagtactgcg ctaagtctct ttcaaagact tcatactgat tcatcaattt
     8461 ttgataatca tcaaagaata ttggagtatc tttttcaata taatcaaaga cagtccatgt
     8521 cttatcatag cacaaagata aaaacttccg agagtctgca tgactttgtt tttggtgaaa
     8581 acttgaaaga atttcttcta ggtatgattt caaaataggt gataaagttt ttgaaatttg
     8641 tttttctaaa gctgactgtc ctcgttgata atccttttct ctcaaaagca tatcactagc
     8701 tggaaagata gtgagttctg tcttattttc tttcgataat tgtgtttcta cttcaaatga
     8761 cctgatacca tcaatttcat caccaaaaaa ctcaattcga caaggttcta actgggatat
     8821 ttcaaaaata tctaaaatat ctcctcgaag actaaattcg ccctgagttt gtacttgagt
     8881 aacttttcga tagccatttt cctttaactg atggataaac gcgtgttgat catattcttc
     8941 accaactgag atttttacaa tactatcttt gaatgcattg ggagacggta aaatcaatcg
     9001 acttgctgcg atattacaaa ctaaaatccc tttcttagat gaatcagtca aaaaacgcaa
     9061 ggcttcaacc cgtgaaataa ttttttcctg tgaagacatc aaaaactcca ccataggagc
     9121 atcatctacc aaaaatggat agacgagttc ctcacccaag atagaaataa gatcactaac
     9181 aagtccttct gcttctccat aagttgacgt caataacaca atcctatctt ctttttctaa
     9241 actgcttgca attgcaagag ccttagtaga tgttgataaa ccaagtatta gttgtctttt
     9301 cttatctgtt aaattttgat gccatttttt aatctgatca ttttctgaga ataaatctaa
     9361 taaggtcacc atttatccgt tatacctctg cattgttttc tcaaaatttt tctcttgtaa
     9421 atagtatttt acagaatcgt caactttgtc aacagactgt aaaataccga tataatcatc
     9481 cctgtcaaac ttactcaaaa catgatgaac aactgacata ccatttttag gtcttccaat
     9541 tccaatctta acacggttaa agacctgagt tcctatatgt tgaataatag acttgatacc
     9601 attatgacca cctgctgagc cttttgctct taaacgaatt ttcccaactt ccatgtcaag
     9661 atcatcgtaa atgataagta aatcgtcaat atccaaacca tagtaagtta ataaagcatg
     9721 aactgctttt ccactttcat tcataaaggt cgttggttta accagataaa ttttttctcc
     9781 atttaggaaa aaggatgcta ggtcagcttg aaatatctta tcgtgtgtaa aagtgacatt
     9841 ctgtttcttc gctagttgat caatcaacat aaaaccaaca ttgtgttttg tttcaaaata
     9901 tttatcccct ggatttccca agcctacaag taatttggtc atttattttt cctttcaaaa
     9961 gccaaaaggg ttggaatttt ttttccaacc taattgacac catttattaa tttttagaca
    10021 ttaaagcgga attccatgat atcgccatct tgaacgatat attcttttcc ttcttcacgc
    10081 aagcgtccag cttcttttac ggccttttca gatccgtatt tcactagatc ttcatatgac
    10141 atggttactg cacgaataaa gcctttttca aagtctgagt ggataatacc agctgcttga
    10201 ggagccttca taccacgttt gaaagtccaa gcgcgaactt ctttttcacc agctgtgaag
    10261 taagttccca atccaagcaa gtggtaagct gcacgcgtca acttatctac acctgattct
    10321 gtcaaaccaa tggcttcaag aaactctttt ttatcttcat catccaattc agaaatttct
    10381 tcctcagcac gcgcagaaat aacgactact tcagcatttt ctgtcgctgc aaattcacga
    10441 atttgtttga catagtcgat agagtcaggt tctgaaacca catcctcgtc cacattagct
    10501 acataaagaa ctggtttagt cgtcaaaagg aaaagacctt tgacaacctt ttgttcctca
    10561 tctgtaaatt caatggtacg agctgatttc ccgtcttcta ggactggttt aatcttttga
    10621 agaacattga attctgctac tgattcttta tctttttgcg tacgtgccat cttttctaca
    10681 cgcgcatatc gtttgttcac tgattctaag tcagcaagaa tcaattccag attaattgta
    10741 tcaatatctg caagtggatc tacaaaggcg tcttcacgtc cttgctcgcg catcacattt
    10801 tcatcatcaa aagcacgaac tacgtgaaca atcgcatcta cttcacgaat attggccaag
    10861 aatttattcc ctagcccttc tccttttgaa gctcctttta caatccctgc aatatctgta
    10921 aattcaaatg ttgtgggaac tgtcttttta ggagttatca tttcagttag tttttgtagg
    10981 cgttcatctg gaacttccac cattccaaca tttggatcaa tagtcgcaaa tgggtagttt
    11041 gctgcctctg ctcctgcttt tgtaattgca ttaaataatg ttgatttacc aacgtttggc
    11101 aaaccaacga tacctgctgt caaagccata tttctcattc tccgttttca tttcaatccc
    11161 taatattata acacaaaaag ggataaagtg ctaaccctct aactaagggt tctcagtcaa
    11221 taattttatt catttttcgc tcaaaatcat atcgccccat catgacaaca tgctcacaat
    11281 tactgcattt tattttgata tctgctccta cacgtgtaat ttcccaacga ttagcctttt
    11341 taccagtcga cttgattgta caagcgtgtg attttttcat ctcaacaaaa tttccaactt
    11401 gatacatact actctccttt tctttttcga ttatatcata aaagaggcga gccaggctca
    11461 acctctttca cttaatttgt acgaactggt gtaatgagct gcatgaagtc ttcgtcagta
    11521 tctgctggca caagagtaaa tggacgaaca gctgagataa agctaatggt caccttttcg
    11581 ctatttaaag ctttaagaga atcaatcaag taagttgggt tgaaactaat ggtcaaatct
    11641 tcaccagtaa cctgatcagt atcgatttct tcgtttactt taccaacttc tggagagtga
    11701 acatgggcgc taacaacccc atccttaatt tcaagtttca cagtaccatt ttgagtcgca
    11761 cttgataaaa gacgggcacg ctccattgac tggcgtaagt ttaccacatt aaaagtaata
    11821 gtagtgttaa agtctgttgg aatcaagcga tctgtatcag gatagtttcc ttctaggaga
    11881 cgagtataga agctaatatt ttcgcttcta aagaggattt ggttattggc aaagaaaatc
    11941 tctacagttt cgatatcatc tgtaaatacc gctgaaaatt cgcgtagaga acggctagga
    12001 attacgacat caaaatcatc actatttttt tcaagagtca attttttctg gcttaggcga
    12061 tgagagtctg ttgcaactgt ttttaactct ttgtgttgac tcaatacgaa gtggacacct
    12121 gttaaaatcg gacgactctc ttgtgtactt gcagcaaagg ctgtttcatt aataattttc
    12181 ttgagtaatt ttgtttcaag tattaaagga gtgcttgctg aaatttcttg gattcgtgga
    12241 tattgttcgc tatcttttcc ttttagggta atttctgatt tgccactggt taaaacaatt
    12301 tgattttgtt caatttcttt aaaatcaaga gtcacatcag gtaaactaga tactacattg
    12361 ataaagaaag aagcttcaag aaggatcgaa cctaaagaag taattaacaa accagcatct
    12421 tcattttttt gagaaataaa attttcaatt gaaatttgac catttgaacc aattaaagta
    12481 ataccttcat tggtcacgtc aatttttact gttgataaaa taggaatggc atttttagaa
    12541 ctaatagctc tcttagtagt atttaatgct tgtagaaata aatttttatt aattgaaaaa
    12601 tgaatcatgg attctccttt atttattttt agtattagaa agataatagt aataatagag
    12661 tctgtgaaat ctgtggaaaa cagcctttaa acaagtcata tcaagttttt tagcttgttt
    12721 aaaaaatgtg gataaaaaag ataaaaaact aaaagttatc cacaaattat ttgattttct
    12781 ttttgattga ttcaatttct aaacgtaaat tatcgtcttg atcaatcaaa gattttattt
    12841 tggcatgggc atgaatgact gtggtatgat cttttccccc aaattccttc ccaatttttg
    12901 gaagactatt atctgttagt tctctagata aatacatggc tacttgacgg gccaaaacaa
    12961 tattttgaag gcgtctactt cccttcattt ctttgatact aacaccataa aagttaccaa
    13021 cttcagtttg gattttatca attgggatga cgagcatttg gctaacatct tgtttgcggg
    13081 ctctaatggc ttctgcagca atatcaatag tgatatcctt gatttttttt actctggcaa
    13141 ttaaagtgat gtcgttgatg gctccctcaa gatctcgaac atttgaatca aattgcccag
    13201 ctaggtattc tagagtatca ctttggaaat tgtagcctaa atgttccgtt ttactttgta
    13261 aaatggcaat acgtgtttca aagtcagggg gtgtgatagt ttgtgtcaat ccccaactaa
    13321 aacgcgtgac aagcctctcc tcgagccctt ctagatgttt tggactacga tcactcgtta
    13381 ggacaatctg tttttgcttg tcatgaaggg cgttaaaggt attgaaaaat tcttcctgag
    13441 ttgcgacttt ttttccgctg agtgactgta tatcatcgat taacaaaaga tcaagactac
    13501 gataggtctt tttaaacttt tccatttccc caagtcttag gtgatcaaga aagtcattaa
    13561 taaagctttc ggcagggata tatttaacac gcgcattagg aatatttttt agaatttcat
    13621 ttccaatagc gtttaataag tgagtcttac caaggcctgg tcctccatag ataaaaagag
    13681 ggttataggt cagagccaaa tcttcagaga cagctaaagc ggctgataca gcccaaacat
    13741 ttccatcccc ttgaataaag ttatcaaagg tatacttttc ttttaatccc gtatctgaat
    13801 aaggaataga tactaacttt ggactatagt cataaagagt taaatttgta gcttcttcaa
    13861 cttgtgagct agtcgtatct tgaggtttgg tgaaaatata gtggggagtt atttcagcgt
    13921 cataaatttc aaaaccagct actacaataa tatcttttag ttgtttttcc cagaccattt
    13981 ccatttcaga gcgaggtaga aatatagtgg caacattttc ctctaccttg atgagttcag
    14041 cttgaatagc atagaaatca tacatggatc gagtcagtct ttcttgtgca aattctaata
    14101 tacgattcca aaattgtttt tctttcaatc ctttctcctc ctttactatt taaaagttta
    14161 atactagaga tattttacca tagatagcaa gaattttcca caagctgtga aaaataatcc
    14221 acaatgttac taaactttat ccacaggttg gggataaaag aagaaattat tgatttatta
    14281 agctttttat cgaaaaacat agtggataac cttgtgagat aaaaaaataa aagaacagcc
    14341 ttatttcagg ctgttgataa ttctactata ttcttcttga tttgaaaaag aaataatgat
    14401 ttttccactg tcttttttag atagtttaat ttctacatct aatccgagta gttttcttaa
    14461 ctgtttttct tcattttgta tgaaatgatt agttttttgc tgtttctttt gttttttctc
    14521 tgtcagaaga gcttctaact tccttacaga aatatcttct tctataatcc gttgaaagaa
    14581 atagtcttgt tgttccttat ttaacccaac tagggaacgc gcatgggctt gtgatagttt
    14641 gccattttct acttctgaga gaatctgttc tggcaaggaa agtaaacgaa tggagttgct
    14701 gatatatgga cgagacttgc ccatcttatc tgcaatttca gcatgggtga atcctttctc
    14761 tacgagagat tcataggcgc gtgcttcttc tattgggttt aaattttctc tctgtaaatt
    14821 ttcaataatg gactggacca tcatctcttg gtctgaaatc tgtttaacaa cagctgggat
    14881 agaccgtaga ccagctaaaa gtgaagcccg atagcgtctc tctcctgcaa ggatttcata
    14941 accaataaca ggagattgac gaacaataat cggttgaatg accccatttt ctttgataga
    15001 ctgtgctagt tcatctagtt tttctctatc aaattctttt cggggttgat agggattttt
    15061 ttgtatatct gtgatagaaa tcatttcaaa tttttccatg attctacact aacacatctt
    15121 ttctcttatg taaagctttc tttacataga tgtcaattaa gattctaaat cacctgaact
    15181 cttgttaagt ttgatagagg tagtttcttc tttcccgtta cgatagtagg ttatcttaat
    15241 ggtgtctccg atagaatggt tgtaaagagc actttgtaaa tctgttgatg aagcaatctc
    15301 tttgtcatct acttttgtaa ttacatcgta tttttcaagg tgaccattgg caggcatatt
    15361 actttgtacc gaacgaacaa ctacaccaga tgtaacatta cttggaatat tgagtcttct
    15421 gatgtcgctt gtactcacat tagataaatt aaccatctgg attcccaaag ctggacgagt
    15481 cacttttccg tttttttcta actgttcaat aatattgata gcatcatttg caggaattgc
    15541 gaaaccaaga ccttctacag atgttcctcc atttgtagca attttacttg aggtaattcc
    15601 gataacctgc ccttgaatat tgatcagtgg gccgccagag ttacctgggt taatagcagt
    15661 atcagtttgg atggcttttg tagaaatagc ttgtccatct tccgatctta aggatacatt
    15721 tctattgaga ctggatacga taccttgagt gacagtattt gcatattcag aacctaacgg
    15781 gctaccgatg gcaatagcag tttctcctac agttaactta ctagaatcac caaactcagc
    15841 tactgttgtc actttttctg aagagatttt gacgacagca atatcagaga aagtgtcagc
    15901 tccgacaatt tctccaggta ctttagtccc atctgacaat cgaatatcta ctttgctggc
    15961 gccatttata acgtgattgt tggtgacgat gtaagcttct ttatcattct ttttataaat
    16021 aactccagat ccttcactag agattcgctg agaatctgtg tcagtatcat cattgccaaa
    16081 tacgctattt tgtctgtttg ccgaataagt aataacagaa acaacagcat cttttacttt
    16141 gttaacagcc tgtgttgttg aattttcgtt cttataggca gtttgtgtaa tagtactatt
    16201 gttgttagag ttgtttacac tacttttttg agttagttga gttattgaaa aactacccaa
    16261 ggctccacta aaaaagctaa tgacgataac gactaataat tgaaaccatt ttttgtaaaa
    16321 tgtttttaga tgtttcatat ttgcctccat atgtttgaat tactgaaagt ataaactgac
    16381 tagcttaatt ataacttaaa cacaaaagtt ttacacaaac tgtggataac tcttttgaaa
    16441 ctgtgatttt cttaattgaa atctattttt tattttgtga ataagatgtg aaaaaataga
    16501 gaatatgtta gaatagagtc atgaaaatta aagttgtaac agttgggaaa ctgaaagaaa
    16561 agtatttaaa agatggtatc gcagagtatt caaaacgaat ttctagattt gctaagtttg
    16621 aaatgattga gttatcagat gaaaaaacac cagataaggc cagtgaatca gaaaatcaaa
    16681 agattttaga aatagaaggt cagagaattt tatcaaaaat tgctgaccgt gatttcgtta
    16741 ttgtgttagc cattgaaggg aaaactttct tctcagaaga atttagtaag cagttagaag
    16801 aaacttctat aaaaggattt tctactctta cttttattat tgggggaagt ttaggattgt
    16861 catcatctgt aaaaaataga gccaatcttt ctgtcagttt tggtcgccta accttacctc
    16921 atcagttaat gagactagtt cttgttgaac aaatctatcg cgcttttacg attcagcagg
    16981 gattccccta ccataaatag aaaattgact tttaattgaa tttttggtag aataattgtg
    17041 ttaggtctca tagctcagct ggatagagca ttcgccttct aagcgaacgg tcgcaggttc
    17101 gaatcctgct gggatcaata taatagcaaa gctgggaatt ttcccggctt ttttcttaaa
    17161 aaagtacact ttgggagaaa aaaatgacag ttgagagaat tttatctaaa acgaaattcc
    17221 attttgtata atggtttttg taagttagct tacaagaaaa aaacatttta ggagatttta
    17281 ttatgaaaaa cacagttaaa ttggaacagt ttgtagcttt gaaggaaaaa gacttacaaa
    17341 agattaaagg tggggagatg aggctgtcaa aattcttccg tgattttatt ttacaaagaa
    17401 aaaagtaatg aaataagggg aaagagtaat ggatttattt ggatttggga cagttattgt
    17461 tcatctttta attattagtc acaactacca tttaatttgt aaaggtcaaa taaatagaaa
    17521 agaattattc gtttttggtg cttatacatt actaacagaa atagtatttg attttccctt
    17581 atatattcta tatttagatg ggttagggat tgcaatattt ttatttcctt tgggcttata
    17641 ttcctatttt cgatggatga aacagtatga gagggataga ggactattcc taagtttact
    17701 actatctctt ttatatgaga gcactcataa ctttctgtcc gtaactttct cctctataac
    17761 aggagataat tttgttttac aatatcattt cccattcttt ttcgttgtga cggtgttaac
    17821 ctattttgtt acattaaaaa tcatttatta tttccatttg gaactagcct attttgacaa
    17881 ggactacctt tatcctttct tgaaaaaagt attttttgct ttactattgc tacatattgt
    17941 atctttcgtt tcagatatgg taagtacgat taaacatttg aatagttttg gaagtatttt
    18001 gtcatctatt gtctttatct ctctcctttt gaccttcttt gcaatgaatt ctcataaagt
    18061 tcaaatggag aaagagattg ctttgaagca gaagaaattt gaacagaaac atttacagaa
    18121 ttacacagat gaaattgtcg gtctgtataa tgaaatccgt ggttttcgac atgattatgc
    18181 tgggatgctt gtcagcatgc agatggcaat tgacagtggt aatttacagg aaattgacag
    18241 aatttacaat gaagttttag tcaaagcaaa tcataaattg cgttcagata agtacactta
    18301 ctttgatttg aacaacatag aagactcagc tttacgaagt ttggttgctc agtcaattgt
    18361 ctatgctcga aataatggtg tagagtttac actggaagta aaagatacga ttaccaagct
    18421 tccaattgaa ctattggatt tggttcgtat catgagcgtt ttattgaata atgctgtcga
    18481 aggatcggct gatagctata aaaagcagat ggaagtagca gttattaaga tggaaactga
    18541 aacagttatt gtgattcaga attcatgtaa aatgacgatg actccttcag gagatctatt
    18601 ttccttagga ttctccacta agggaagaaa tcgcggagtc ggattaaata atgtgaaaga
    18661 actactagat aagtacaaca atattatttt agaaacagag atggaaggca gtacatttag
    18721 acaaatcatt agatttaaga gggaatttga atgaaagttt taattttaga agatgttatt
    18781 gaacatcaag tgagactaga gagaatattg gatgaaattt cgaaagaatc gaatattcca
    18841 atatcataca agacaacggg aaaagtccgt gaatttgaag aatacattga aaatgatgaa
    18901 gtaaatcagc tttatttcct agatatcgat attcatggaa ttgagaaaaa gggatttgaa
    18961 gtggctcagt tcattcgtca ttacaatcct tacgctatta tcgtctttat cactagtcga
    19021 tcagagtttg cgactctaac ctataaatac caggtatcag ccctagattt tgttgataag
    19081 gatatcaatg atgagatgtt taagaagaga attgagcaaa atatcttcta cacgaagagt
    19141 atgttacttg aaaatgaaga tgttgtagat tatttcgact acaattacaa gggaaatgat
    19201 ttaaaaattc cttaccatga tattttgtat attgaaacaa caggggtatc tcataaattg
    19261 cgcattattg gtaagaattt tgcaaaagag ttttatggta ccatgacaga tattcaggaa
    19321 aaggacaaac atactcagcg attttattct cctcacaagt catttttggt aaatataggc
    19381 aatatcagag aaattgatcg aaaaaactta gaaattgttt tctatgaaga ccatcgttgt
    19441 cctatttcaa gattaaaaat tagaaaatta aaagatattt tagagaaaaa atctcaaaag
    19501 tgattgacaa ttagcaagaa attgatataa tggttatatc tgctacagat agaaggtccg
    19561 ttggtcaagg ggttaagaca ccgccttttc acggcggtaa cacgggttcg aatcccgtac
    19621 ggactatttt atccgccata gctcagttgg tagtagcgca tgactgttaa tcatgatgtc
    19681 gtaggttcga gtcctactgg cggagtatag ataaaaggga acacagctgt gttcctcttt
    19741 ttgtatcaat ttgtatcacc aagcattttc ataaggaagt ctgttatttc ttgagaactt
    19801 tctttttttc catgtgcaat ccaagtttgg cagacaccaa aaagtgcatg agttagatag
    19861 atgctactat attctaattc agtggtattt agattcagtt gcataaatcg cttttgtaaa
    19921 tctgtactga gcatgatatg aagtttattt cgtaagaaat tttggatttc tttagtccca
    19981 ttttcagaaa gaagggcagc cagaagtggt tctgactcta gatattcaaa aacttctaaa
    20041 atagcgtctc ttttgtgatg agcatgtttt tgaaaaatat attcaaatgt atggaatagc
    20101 ttgctttgat agtgctcaat catatcatac ttatccttat agtgagtata gaagctggaa
    20161 cgactaattc cggctttttc tactaatttg acagtagaaa ttttatcaaa tggctgttcc
    20221 atcagtaatt gtaccatagc gttttcaata gttcgctttg tttttaagcg tttgttactt
    20281 tcttgcatat ttcctccttg taaacaaatt agactatatg tctaaaaata gattttttat
    20341 cttgtaattt agatttttta atgtataatc tattatatca aaattttaga caatatgttt
    20401 aaaaaaggag aaactatgtt taaagaatgg aaagcaattt aaaaaaaaac aacctttatt
    20461 attgtcatga tcgggatttc tcttattcca gctctgtaca atatcatatt tttgtcatca
    20521 atgtgggagc catatgggca attgtctgac ttacctgtgg cagttgtaaa taatgataaa
    20581 gaggcttcct ataatggtaa tactatggca ataggaaaag acatggtgtc caatttaaaa
    20641 gaaaataaaa ccttggattt tcattttgta gatgaagagg aaggaaagaa gggattggaa
    20701 gatggcgatt actatatggt agtgacttta ccaagtgatt tatctgaaaa aacaactaca
    20761 ttatccaata ttcaatcgac agcagcttat caatcattga caagtgagca acaaactgag
    20821 ataagtgatt ctgtatctca aaattcaact gatagtattc aatcggctca gtcaattgta
    20881 gctttagtac aaggtttaca gggaagttta gaaaacttac aaaatcaatc ttctaatctt
    20941 tcgactttaa aaaatcaagc taatcaagta ttacctatta cttctacttc tttgatagga
    21001 ttgtcaagtg gattaacaga gatacaagga gatgttacta gcaaattagt tcctgccagt
    21061 cagtcgattg catcaggtgt aaacgcatat actacaggtg ttgataaagt ttctcagggc
    21121 gcaagtcaac taagtgaaaa aaatgccacc ttgacaggta gtttggataa actagtttca
    21181 ggctcaaaca ccttgacaca aaaatcttct agattgacag caggagttgg ttaattacaa
    21241 tcaggatctg ggcaattagc agacaaatcc agtcagttac tttcaggtgc ttctccatta
    21301 gagaatagag ctaataaatt ggcagatgga tctaggaaac tagcagaagg tggaacaaag
    21361 ttaacttctg gattggaaga tttacagaca ggacttgctt ctttaggaca aggactaggt
    21421 aatgctagtg atcaactcaa atcagtatca acagaatcta aaaatgcaga gattttgtca
    21481 aatccactca atctttcaaa aacagacaat gatcaagttc ctgtaaatgg aatcgcaata
    21541 gctccttata tgatatcagt tgctcttttt tttgcagcaa tatcaacaaa tatgatattt
    21601 gcgaaattgc cttcaggacg tcatccagag agccgttggg cttggttgaa atcttgagct
    21661 gaaataaatg gtattatagc tgttttggca ggaattttgg tatatggagg agttcagctt
    21721 attggtttaa ctgctaatca tgagatgaga atatttattc tcatcatcct aacaagttta
    21781 gtattcatgt ctatggtgac cactttagca acgtggaata gccgtatagg agcttttttc
    21841 tcacttattt tgcttttact acagttagca tcaagtgcag gtacttatcc acttgctttg
    21901 acaaatgatt tctttagatc tattaatccc tggttaccaa tgagctattc agtttcggga
    21961 ttacgacaaa caatctctat caacaagtca ttttcctagc tgtcatacta gctctattta
    22021 ctggtttagg tatgctagcc tatcaacata agaaaatgga agaagattaa aaaaatcgac
    22081 cgattaactg gtcgattttt tatgccttag atgactttcg tctgtgatta tagattccaa
    22141 atagtaagag agaagtaaag gaacagattg ctccagtaat aaaaccattg ggaatgaagg
    22201 aaagtgtaat agttcctttc cccttgggaa tgtcaacttt cataaatcca gtttgagctt
    22261 gtttaatttc tattttctta ccatcttggt aggcagacca acctttgtca taaggaatgg
    22321 tgaagaaaat agatgtatct tgttggacat catatgtagc aaaaaccttg tttttagaag
    22381 ttgatactgt gacaggttgt tctttaattt tttgaattgc ctcggtgaaa gttttggtat
    22441 ctaaacgata gaaggtagga gattcaaatg atacttgtga atttccaggg aaactaacat
    22501 tgatattgaa agtttttttc tctttagtat atcctagatt aaagaaggag aagacattat
    22561 cagttgtaaa agtctttttt tcaccattta caaggatgtc aaccttcttt tgtttatcgt
    22621 tagaaaagtg aaggtttatg aaagagagat aaacttggct gttttctgga acttcaattt
    22681 gatactggat tgctgcatct tcatttgaag aacttgtgac actaatcaaa tcattagtat
    22741 tttctatttt ttctgttttt tcataaggta ttggagaaaa ataatcaaaa ttgacgttag
    22801 caagttgatt taaaaatgag gcctgattat ccaaggtatg ttcattgaac ttgacatcat
    22861 tgtaaacaga ttgactagca actgcaatcg gaagagagta ttgattttca tatagggtaa
    22921 gattatcttt ttgatagata tctttaaagc catacttatc aataggactg tctgagatat
    22981 tgtactggat accaaataaa ctatcagcca aaatactatt atttgcatat cggagattga
    23041 gattagtccc agaggattta aaaccaagtt tatctaaagt agagcttgat gaacgatttc
    23101 gaacagatga aaattgagag attccattgt agttgaattt catactgtca tttcctgtct
    23161 gagtttgtag tttttcagta cgagtaaatt gatttccaat atatgttgag aaagattcca
    23221 tagctgggat atctcgacta taagcacttc gagaagcaaa tccccattcc ttagcaattc
    23281 cgtccatttg agatgaagca tttaaactca tttcaaccag tataaataaa gagattagaa
    23341 tggcaaatag attcacagat ataaactttt tgataactgc aaggagtaaa agagaataga
    23401 caaccaaaaa ttcaagagta agcagaatat tcaaatctgt taaaaaagaa taatgcgatt
    23461 ttagatagat ggtagctaaa aatcctgcta ctacaagaaa aagcgaaact aaaaaattcc
    23521 agactttaag ttctttcaga cgatttaaga cttctgctgc tgtgtaaatt aacaaggtag
    23581 agaaaatcca agcatagcga tgtaaaaaca tgtttggagt atgcatgcct tgccaaaata
    23641 agtcaagagc ttctatgtaa aagcttgcaa ttagaaatgc aaagaatatt acatatatga
    23701 gtttcacgtg aaacttaata gatttcagcg taaaaaataa aatggtcaaa ataaagggaa
    23761 gtagtccaac aaaaatcatt gggatggccc catactttgt tgtgtcaaag gaaccaatga
    23821 attgcttagc aaagagatca agataccagc tactttcagt ttgaaacttt gtaacttcag
    23881 tcaatttttc cccatgtgtc tgtaaatcaa acagagtggg aagagtcata atcaaactag
    23941 ccataccagc taaaaaggag ataactatga aatcaagaac agatgatttt cgagtcttaa
    24001 agtcccacga aatttgacag agataccaga aaataagaaa caatactgtc atatatccaa
    24061 aataataatt ttgaataaat aagattgaca gacttgtaaa gtacaatagg agtttctttt
    24121 cagttatcag tagatgtaaa ccagttataa ttaaaggaat caagataaaa acatctagcc
    24181 aggtttttat ctctaattga ctgacagtga aactcatcag agcataggaa gtagataagg
    24241 ctagttttaa aatctgaggg atagattgaa acaatttatt caaactaaaa aaggttgaca
    24301 gaccaatcaa tccaaatttt aagagagttg tcagatagat agcatctggc atattcgtta
    24361 gatcaaaaaa gtaaaccaga ggcgcgagaa aactacccaa gtaataacta gatagggcat
    24421 agaagtttag ccctagacca cttgtaaagg tgtaaaacag attactattt ccatgtagga
    24481 tatttcgtaa ggctacatca aaaataacgt attgatgaaa gccatctcct aatagaggag
    24541 agttgtcgct attccagtag ataccttgag atagatatac tccagacata atcactacag
    24601 gaatgatgaa agaaataaaa taggttcgat atgtttttaa aaatgatttc atgttacctc
    24661 gtagaatgat agaaaactca gttggttaac ccaactgagt tttgaagttt tatttagtct
    24721 ttccaaagtt ctttaacttt tgcttgtact tctgcatttt ctaggaattc atcgtaggtt
    24781 tcatcgatac ggtcaatgac gccattttta gataagacaa tgatatggtt agccaaagtt
    24841 tgaataaatt cgtggtcatg gctggcaaag atgattgatt ctttaaagtt tttcaatcca
    24901 tcattcaagc ttgagataga ttccaagtcc aagtgatttg ttggatcatc aagcacaagg
    24961 acatttgatt ttaagagcat gagttttgaa agcatgacac gaactttttc tccccctgac
    25021 aagacattta caggtttgtt aacttcatct ccagagaaga gcatacggcc gaggaagcca
    25081 cgtaggaaag tattgtcatc ttcttcttta cttgcgaatt gacgcaacca gtcaagaatt
    25141 gattctcctc ctgcaaaatc agctgagtta tcttttggta ggtaagattg actagttgta
    25201 actccccact tgacagttcc ttcatagtca atatctccca tgattgcacg aattaatgca
    25261 gtcgtttgaa tatcattttg tccaataagt gctgtcttat catctggacg caagatgaaa
    25321 ctaatattat ccaagatagt ttcaccatca atctttacag ttagattttc tactgtcaag
    25381 agatcattac caatctcacg ttccgcttta aagttgataa atggatattt acgactagat
    25441 ggtacaatct cttctagctc aatcttatca agcattttct tacgtgatgt tgcctgcctt
    25501 gacttagaag cattggcaga gaaacgagca acgaattctt gcaattgttt aattttttct
    25561 tctgctttag cattacggtc tgctagcaat ttagcagcaa gctcagaaga ttccttccag
    25621 aagtcgtagt ttccgacata gagtttgatt tttccaaagt caaggtcggc catgtgagta
    25681 caaactttgt ttaagaagtg acggtcgtgg gatactacga taactgtgtt atcaaagtca
    25741 atcaagaagt cttctaacca agtaatcgat tggatatcca aaccgttagt aggctcgtcc
    25801 aagagaagaa catctggttt accaaaaagt gctttggcga ggagaacctt tactttttca
    25861 ccgttggcca attcgctcat gttttggtag tgtaattctt ctggaatgtt taggttttga
    25921 agtagttgag aggcttcact ctctgcttcc caacctccaa gttcggcaaa ctctccttcg
    25981 agttcggcag cacgaacccc gtcctcgtct gagaaatctt ccttcatgta gatagcatct
    26041 ttctctttca tgatgctata aagtttttca tttcccatga taacgacatc aatggcacgt
    26101 tcatcttcgt agtcaaagtg attttgacga agaacagaga gacgttcatc tggaccaaga
    26161 gagatgtgac cagtagtagg ttcgatatct ccagctaaaa tttttaaaaa ggttgatttt
    26221 ccggcaccat tagcaccgat taatccgtaa gtatttcctt ctgtaaattt gatattgaca
    26281 tcatcaaaaa gtttgcgatc actaaaacgt agtgaaacat cagatactgt aagcaatatt
    26341 tttctcctat atgtgtaata tatttattct actagaaaat acagaaatat tcaaattttt
    26401 atttgtcaat tttgtgtaaa ttatatttac agtatccttt acacaaatct gtaaaaagca
    26461 aggctgattt attttgataa attacggtta tttcattaaa aaaatgctat aattgaaagg
    26521 actatatcga aggagaacaa aatgactaaa cccattattt taacaggaga ccgtccaaca
    26581 ggaaaattgc atattggaca ttatgttgga agtctcaaaa atcgagtatt attacaggaa
    26641 gaggataagt atgatatgtt tgtgttcttg gctgaccaac aagccttgac agatcatgcc
    26701 aaagatcctc aaaccattgt agagtctatc ggaaatgtgg ctttggatta tcttgcagtt
    26761 ggattggatc caaataagtc aactattttt attcaaagcc agattccaga gttggctgag
    26821 ttgtctatgt attatatgaa tctagtttcg ttagcacgtt tggagcgaaa tccaacagtc
    26881 aagacagaga tttctcagaa aggatttgga gaaagcattc cgacaggatt cttggtctat
    26941 ccaatcgctc aagcagctga tatcacagct ttcaaggcta attatgttcc tgttgggaca
    27001 gatcagaaac caatgattga gcaaactcgt gaaattgttc gttcttttaa caatgcatat
    27061 aactgtgatg tcttggtaga gccggaaggt atttatccag aaaatgagag agcagggcgt
    27121 ttgcctggtt tagatggaaa tgctaaaatg tctaaatcac taaataatgg tatttattta
    27181 gctgatgatg cggatacttt gcgtaaaaaa gtaatgagta tgtatacaga tccagatcat
    27241 atccgcgttg aggatccagg taagattgag ggaaatatgg ttttccatta tctagatgtt
    27301 tttggtcgtc cagaagatgc tcaagaaatt gctgatatga aagaacgtta tcaacgaggt
    27361 ggtcttggtg atgtgaagac caagcgttat ctacttgaaa tattagaacg tgaactgggt
    27421 cctattcgtg agcgccgtat tgaatttgct aaggatatgg gagaagttta taatatgatt
    27481 caaaaaggta gtgaaagagc gcgtgaagtt gcgggtcaaa ccctatctga ggtaaaaggg
    27541 gcaatgggac ttcattactt taactaagtt tattttacaa gaaactatca tttctatctc
    27601 gaagaaaaga tagttttttt atttacccct gtaacatttg acaaattttt atagaatggt
    27661 atgatagata caatataaaa agagtcaagc tcaaaaagaa agaaaagagg aaacttcgaa
    27721 tgtctaattg ggacactaaa tttttgaaaa aaggttttac ctttgatgat gtattgctta
    27781 ttccagctga aagtcatgtg ttgcctaacg atgcagattt aacaactaaa ttggcagata
    27841 atttgacttt aaatatccca attattaccg ctgccatgga cacagttaca gagagtcaaa
    27901 tggccattgc tattgctcgt gcaggcggtc tcggagttat ccataaaaac atgtcaattg
    27961 ctcaacaagc agacgaggtt cgtaaggtaa aacgttctga aaatggagtt attattgatc
    28021 cgttcttctt gacgcctgaa catacaattg ctgaagcaga tgagcttatg ggtcgttacc
    28081 gcatcagtgg tgttccagtt gttgaaacac ttgaaaatcg taaattggtt ggtattttga
    28141 caaaccgaga tcttcgtttt atttcagatt ataatcaacc aatttcaaac catatgacta
    28201 gtgaaaatct tgttactgct cctgtgggta cggatcttgc aacggctgag agtattcttc
    28261 aagagcatcg tattgaaaaa cttccgttgg tcgatgaaga aggcagtctt tctggtttga
    28321 tcactatcaa agatattgaa aaagttattg agtttccaaa tgcggctaaa gatgagtttg
    28381 gtcgtcttct agttgcaggt gcagtaggtg ttacttcaga tacatttgaa cgtgcagagg
    28441 ctctttttga ggcaggagcg gatgcgattg ttattgatac tgcacatggt cattctgcag
    28501 gtgtcttgcg taaaattgcc gagattcgtg ctcatttccc agatcggact ttgattgctg
    28561 gaaatattgc tactgctgaa ggtgcacgtg ccctttatga agcgggtgta gacgttgtta
    28621 aggttggtat tggaccaggt tctatctgta ctactcgtgt gattgctggt gttggtgttc
    28681 cgcaagtaac agctatctac gatgctgcag ctgttgcgcg cgaatatggt aaaacgatta
    28741 ttgctgacgg tgggatcaag tattctggag atattgtaaa agcacttgct gcaggtggaa
    28801 atgctgttat gcttggatct atgtttgctg gaactgatga agctccaggc gaaactgaaa
    28861 tcttccaagg acgtaaattc aagacttacc gtggtatggg atcaattgct gctatgaaga
    28921 aaggttcaag cgaccgttat ttccaaggtt ctgtcaatga agcaaacaag ctcgttccag
    28981 aaggaattga aggtcgtgtt gcttataaag gagcggcagc tgatattgtt ttccaaatga
    29041 ttggtggtat tcgctctggt atgggttact gtggtgcagc taaccttaaa gaactacacg
    29101 ataatgctca atttattgaa atgtctggtg ctggtttgaa agaaagccat cctcatgatg
    29161 tgcaaattac taatgaggca ccaaattatt ctatgtaaaa aacaatgaaa agaactccag
    29221 tgaaaacagg agttctttta caatgttgtc aatttccatt tacagcagcc ttaccatcct
    29281 gaatagtgaa gatacttaga ttttctggca gattttgaag atggtctaag cttgttgttg
    29341 tgataaaggt ttggattgat tgagaaatcg tttctaataa ttttaactgt ctagtgttgt
    29401 caagttcact catcacatcg tcaagcagta atatcggaga ttctgtggta atgctttcca
    29461 ttaattcgat ttctgctaat tttatcgaga ggacgagact acgatgttga ccttggcttc
    29521 cgaaactagc atccatccca tttatataaa aagaaatgtc atctcgatga ggaccgacac
    29581 cagtattctt tttaaataaa tctctggatc tacttttttc taaagcaatt ttgaaagatt
    29641 cggataagtt ttgtttgtca gttatattga cagaagattg ataggatatt gacaactctt
    29701 cgatctgatt agagagttca aaatgtttct tacgcccaaa tgattctagt ttttttatga
    29761 aatctaagcg gtgattcatt acacgacatc cataatcaac tagctgatca tctaacacag
    29821 aaaggaatgt ttcatctatt ttttgagctg attttaggta agtgtttctt tgctttagga
    29881 tgtggttata attggttaag tcagataaat agattggctt aatttgccca agttccatat
    29941 caatgaattt tcgtcgaatc gaaggtgctc ctttaattag ttgtaaatct tcaggagcaa
    30001 ataagacaac attcatgtgt cctacataat ctgaaaggcg tgcctgtttt aagtgattaa
    30061 cttttgttac acggcctttt tgtgttagtt cgatttctag gggaatggat ccagtttttt
    30121 tctgaacgag acctgaaaga tgaagttgtt cctcatcaaa atgaatgaga tttttatctg
    30181 ttcgagttcg atgactacgc gttaaggcta aaaaatagat agcctctaac atgtttgttt
    30241 ttccttgtgc attacgtccc aaaaagacat tcaatttagg attaaagtct attttcgttt
    30301 ctttgtaatt acgaaaagtc ttgagagata gatgttgtag ccacatgatt atctaccagg
    30361 gaatcgtgga gcttgttggc ttttaggtga tgaagcaggt ttcgatttgt cttttttaac
    30421 tcccttattc atctttttga caagtttagc gatccgttct ttttcaactt tatcagcttg
    30481 gtattcatct tgttcttcag aagtaggttg tgtcaacaag atgtcaatgt tcatgtcagg
    30541 gatgtcaact ttatcgccaa tacgaagttt ttttccacga cgactctcta attccctatt
    30601 aaagtaaaca gaatgttcag agagaaatga tttaatagct cctccgctat gtgtaattcc
    30661 aagttctttg agtagtgctt ggagggtaat aaattcttca aataatttgt attccataat
    30721 tcacctcaat ctttggtatt ataccatatt ttcctaaaaa tagtgagaga gacagcgaga
    30781 aatgtaagcg atttttattt caaaagtgtt acagggaaca agaaaatttc aggttttcgt
    30841 gatataatag aagtctgtat ataaggaggt aaatcatgga gttagtgcat ggaatttcaa
    30901 cacattttat ccaatcaaaa aagtttaaaa caaacaaaat taccgtgcgt tttaccgctc
    30961 cattatccct tgatacgatt gcaggtcaca tgttgagtgc aagtatgcta gagactgcta
    31021 atcagatgta ccccacttct caagatttga ggagacactt ggccagtcta tacggtacag
    31081 atatgtcaac caattgtttc agaagagggc aaagccacat tatagaattg acatttacct
    31141 atgttcgtga tgagttttta agtaggaaaa acgtgctaac ctctcagatt ttggaacttg
    31201 taaaagaaac tcttttttca cccgtagtag ttgataatgg gtttgatccg gccttatttg
    31261 aaattgagaa aaaacaattg ctagcaagtt tagcagctga tatggatgat tctttttatt
    31321 ttgcacataa agaattggat aaattgtttt ttcatgatga acgtcttcaa ttggaatata
    31381 gcgatttacg aaatcgtatt ttagctgaaa ctccacaaag ttcttattct tgtttccaag
    31441 aatttttagc caatgatcga atagatttct ttttcctagg tgattttaat gaggttgaaa
    31501 ttcaaaatgt attagaatca tttggcttta aaggtcgaaa aggagatgtg aaggttcagt
    31561 attgtcaacc ttattctaat atccttcagg aaggtatggt tcggaaaaat gtgggacaat
    31621 ccattttgga attaggttat cattaccgtt ctaaatatgg tgatgagcaa catttaccca
    31681 tgattgtaat gaatggttta cttggtggat ttgctcactc taagctcttt acaaatgtcc
    31741 gtgaaaatgc tggattagct tataccattt caagtgagct tgatttattt agtggattct
    31801 tgaggatgta tgctggtatc aatcgagaaa atcgtaacca ggctcgtaaa atgatgaata
    31861 atcaactgct tgatttaaaa aaaggttatt ttacagagtt tgagttaaat cagaccaagg
    31921 aaatgattcg ttggtcgttg ttactttctc aagataatca atcttcattg attgaacgtg
    31981 cttatcaaaa taccttattt ggaaaatctt cagcagactt taaaagttgg attgcaaagc
    32041 ttgaacaaat tgacaaagat gctatttgta gagtagctaa taatgtgaaa ctacaagcga
    32101 tttactttat ggaaggaata gaatgacaaa ggttgttttt gaagaaaaat actatccagc
    32161 tgtaaaagaa atggtttatc gaactcgttt ggccaacgga ttgacagttg ctcttttgcc
    32221 taaaaaggaa tttaaagagg tttacgggag tgtaactgta cagtttggtt cgatagatac
    32281 gcttgtcaca gaagttgacg gagatgtaaa acaatatcct ggaggaattg ctcattttct
    32341 tgaacataaa ttatttgaga gagaagattc tagtgatttg atgtcggctt ttacgagtct
    32401 aggtgcagat agtaatgcct ttacaagctt tacaaaaaca aactatcttt tttcagcaac
    32461 ggattatttt ttagaaaatt tatatttact tgatgaattg gtaacattag cacactttac
    32521 tgaagcttcc attctgacag agcaggatat tattcagcaa gaacgagaaa tgtaccaaga
    32581 tgatccagat tcgtgtttat tcttttcaac tttagcgaat ttgtatcctg gtacaccttt
    32641 agcaactgat atagttggaa gtgaggagtc catttcccaa atcaatctaa ctaatttgca
    32701 agaaaatttt acaaagtttt acaaacctgt aaacatgtct ctgtttttag ttggtaattt
    32761 tgatgtggag cgagtacagg actattttga aagcaaagaa ctgaaagatt cagattttca
    32821 ggaagtagca agagaaaagt tgtttttaca gcctgtaaag ccaacagata gtatgagaat
    32881 ggaagtatct tctcccaaac tagcgattgg agttagaggt aagcgagaag tttctgaagc
    32941 ggattgctat cgacatcata ttttattaaa attattgttt gcaatgatgt ttggttggac
    33001 ttcggatcgt tttcaaaaat gttatgaatc aggtaaaatt gatgcatcct tatctctgga
    33061 agttgaagta acaagtcgct ttcattttgt catgttgaca atggatacga aagagccagt
    33121 tgctttgtct catcaattta ggaaggctat tcgtaatttt acaaaggatt tagatattac
    33181 agaggaacat ttagatatta tcaaaagaga gatgtttggc gaatttttca gtagcatgaa
    33241 ctctcttgaa tttattgcaa cgcaatatga tgcttttgaa aatggtgaga caatttttga
    33301 tttgccgaaa attttacagg aaattacttt agaggatgtc cttgatgctg gacatcattt
    33361 aatagatgat ggtgacatag ttgattttac aatattccca tcgtagtaac ctattataat
    33421 agacactaga aagaagggat gacaagtatg agaaaaaaaa caattggaga ggttttacga
    33481 ttagctagaa tcaatcaggg attgagttta gatgaattgc agaaaaagac agaaatccag
    33541 ttagatatgt tggaagcaat ggaagcagac gatttcgatc aacttccaag tcctttttac
    33601 acgcgttctt tcttgaaaaa atatgcatgg gctgttgagt tagatgacca aattgttttg
    33661 gatgcttatg attctgggag tatgattact tatgaggaag tagatgttga tgaagatgag
    33721 ttgacaggtc gtagacgttc aagtaagaaa aagaagaaaa aaacatcatt tttaccttta
    33781 ttttatttta tcctttttgc tttatcgatt ttaatttttg tgacttatta tgtttggaac
    33841 tatattcaaa ctcaaccaga ggagccttct ctttctaatt acagtgtggt tcaatcaaca
    33901 agttcaacta gctctgttcc ccactcctca agtagtagtt cttctagtat agaatcagct
    33961 ataagtgtat caggcgaagg aaatcatgta gaaatcgctt ataagacaag taaggaaaca
    34021 gttaaattgc aattggcagt ttcagatgtt acaagttggg tcagtgtttc agaaagcgaa
    34081 cttgagggcg gtgtaacctt atcgccaaag aagaaaagtg cagaagcaac agttgcaact
    34141 aaaagtcctg taacaattac gttaggtgtt gtaaaaggtg ttgatttgac agtagataat
    34201 cagactgttg atttatcgaa attaacagct cagactggac aaatcactgt aacctttact
    34261 aaaaattaag gaaaaacgaa tgaaaaaaga acaaattccc aatctcttaa caataggtcg
    34321 aattctcttt atacctattt ttatctttat tttaacgata ggaaattcga tagagagtca
    34381 tatagttgca gctattatct ttgctgttgc cagtattacc gactatttag atggatattt
    34441 agctcgtaaa tggaatgtgg tcagtaattt tggtaaattt gcagatccta tggcggataa
    34501 gttactagtt atgtcggctt ttattgtgtt gattgagtta ggtatggctc cggcttggat
    34561 tgttgcagtg attatctgtc gtgagttagc tgtgacaggt ttaaggcttt tattggttga
    34621 aactggtgga acaattttag cagcagcaat gcctggaaaa attaaaactt ttagtcagat
    34681 gtttgccatt attttcttgc tattacattg gactttgctt ggtcaagttc tactttatgt
    34741 agccttattt ttcactatct actctggcta tgactatttc aagggtagtg cctatgtatt
    34801 taaagggaca tttggttcga aatgaaatca ataattgatg taaaaaatct ttcttttcgc
    34861 tataaagaaa atcagaacta ctacgatgtg aaggatatta cgtttcacgt gaaacgtgga
    34921 gaatggcttt cgattgtagg gcataatggt agtggtaaat caacgacggt tcgattaatt
    34981 gatggcttac tggaagcaga atccggagag attgtaattg atggccaacg tttgactgag
    35041 gaaaatgttt ggaatatacg tcgtaaaatc ggtatggttt ttcaaaatcc agacaatcaa
    35101 tttgttggag cgactgttga agatgatgtt gcttttggtt tggaaaatca gggactttct
    35161 cgtcaagaaa tgaaaaagag agtggaagaa gctctggctt tagttggcat gttggacttt
    35221 aaaaagagag agccagcgcg tctatcaggt ggccaaaagc aacgtgtggc cattgcaggt
    35281 gttgtagccc taagaccagc tattttaatc ttagatgaag caacgagtat gttggatcct
    35341 gaggggcgta gagaacttat tgggacagta aaaggaattc gaaaagacta tgatatgacg
    35401 gtcatttcta ttacccatga tttggaagaa gtcgccatga gtgatcgcgt attggtcatg
    35461 aaaaaagggg aaattgaatc aactagtagt ccaagggagc ttttctctcg aaatgattta
    35521 gatcaaattg gattagacga tccttttgct aatcaattaa aaaaatcttt gagccagaat
    35581 ggctatgatt tacctgaaaa ttatttgaca gaaagtgagc tagaggataa gctatgggaa
    35641 ttgctctaga aaatgtgaat tttacatatc aagaaggtac tcccttagct tcagcagctt
    35701 tgtcggatgt ttctttgacg attgaagatg gctcttatac agctttaatt gggcacacag
    35761 gtagtggtaa atcaactatt ttacaactct taaatggttt attggtgcca agtcaaggga
    35821 gtgtgagggt ttttgatacc ttaatcacct cgacttctaa aaataaagat attcgtcaaa
    35881 ttagaaaaca ggttggcttg gtatttcagt ttgctgaaaa tcagattttt gaagaaacgg
    35941 ttttgaagga cgttgctttt ggaccgcaaa attttggagt ttctgaagaa gatgctgtga
    36001 agactgcgcg tgagaaactg gctctggttg gaattgatga atcacttttt gatcgtagtc
    36061 cgtttgagct gtcaggggga caaatgagac gtgttgccat tgcaggcata cttgccatgg
    36121 agccagctat attagtctta gatgagccaa cagctggtct agatcctcta gggagaaaag
    36181 agttgatgac cctgttcaaa aaactccacc agtcagggat gaccatcgtc ttggtaacgc
    36241 atttgatgga tgatgttgct gaatatgcga atcaagtcta tgtaatggaa aagggacgtt
    36301 tagtaaaggg gggcaaacca agtgatgtct ttcaagacgt tgtttttatg gaagaagttc
    36361 agttgggagt acctaaaatt acggcctttt gtaaacgatt ggctgataga ggcgtgtcat
    36421 ttaaacgatt accgattaag atagaggagt tcaaggagtc gctaaatgga tagtatgatt
    36481 ttagggcgtt atatcccagg ggattcgatt gttcaccgat tggatccacg tagcaaattg
    36541 cttgctatga tgctactgat tttgattgtg ttttgggcta ataacccctt gacgaatctg
    36601 attcttttta tagcgacagg gatatttatt gccttgtcag gagtatctct ttcatttttt
    36661 attcagggct tgaagtctat gtttttcttg attgccttca caactatttt tcaactattt
    36721 ttcatttcta atgggaatgt tttatttgag ttttcgtttg tgagaatcac ggattatgct
    36781 ttgcaacaag ctgggattat tttttgtcgc tttgtattga ttattttctt ttcaactttg
    36841 ttaaccttaa caaccatgcc tttaagtttg gcatcagctg tcgaagcttt attagcacct
    36901 ttaaagcgtg tgaaagttcc agttcatgaa attggattga tgctgtctat gagtttgcgt
    36961 tttgtcccaa ccttgatgga tgatacgacg cggattatga atgcacagaa agctcgtgga
    37021 gtggattttg gagaaggaag catcgttcaa aaagtaaagg cgatgattcc cattttgatt
    37081 cctctttttg cgacaagttt aaaacgtgca gattccttgg ctatcgctat ggaagcgcgt
    37141 ggctatcagg gtggaaaagg cagaagtcaa taccgacaat tgaaatggac tctaaaggat
    37201 acgctgacca ttcttgttat tctcgtactt ggttgttgtt tatttttctt aaaatcttag
    37261 tagctttcag gaattgataa aaagttactg taacagtttt tgatataaag gtagggatat
    37321 gaaccgtttt aaaaaatcaa aatatgtcat tattgttttt gtcactgttc tgcttgtgtc
    37381 agctctctta gcgacgactt attcaagtac aattgtgaca aaattaggag atggaatctc
    37441 attggttgat agagttgtac aaaaaccttt tcagtggttt gattctgtca aatcagattt
    37501 ggctcatttg acacgaacat ataatgaaaa tgaaagtttg aagaaacagc tttaccaatt
    37561 agaagttaaa tcaaatgagg tggaaagttt aaagacagaa aatgaacaac tgcgccaatt
    37621 gcttgatatg aagtctaaat tgcaagccac aaagacttta gcagcagatg ttattatgcg
    37681 ttctccggta tcttggaagc aggagttgac cttagatgca ggtagatcaa aaggtgcttc
    37741 tgagaacatg ttagctattg caaatggtgg cttgattggg agtgtttcaa aagtagagga
    37801 gaactctact atagtcaacc ttctgacaaa tacggaaaat gctgataaga tttctgttaa
    37861 aattcaacat ggctctacta caatttatgg aattattatt ggctatgaca aggaaaatga
    37921 cgttcttaaa attagccaat taaatagtaa tagcgatatt agtgcgggag ataaggtgac
    37981 tactggtgga ttaggaaact ttaacgttgc tgatattcct gttggtgaag tggttgccac
    38041 aacgcatagt acagactatt tgacacgaga agtaactgtt aaattgagtg cagatactca
    38101 taatgtagat gtgatagaat tagtggggaa ttcataatga gacagttgaa gcgagttgga
    38161 gtatttttat tgcttccttt ctttgttcta attgacgccc atattagcca gcttctgggc
    38221 tcatttttcc cccatgtaca tttggctagt cattttcttt ttctatttct cttatttgag
    38281 acgatagaag tatcagagta tctctaccta gtctattgtt ttgttatagg cttggtttat
    38341 gatgtttact ttttccatct aatagggatt acaactctct tatttatctt attgggagcc
    38401 ttccttcata aattgaatag tgttattttg ttgaatcgtt ggacaagaat gctagctatg
    38461 attgtgctga cattcctgtt tgaaatgggt agttatcttt tggcttttat ggtagggttg
    38521 acagtagata gcatgtcgat ttttatagtc tatagcttgg taccgacgat gattttaaat
    38581 tttttatgga ttactgtttt tcaatttatt tttgaaaaat attatctata agaacgacat
    38641 ataaatgtaa caaaggcgta atatttatta ggcctttttt tggtatacta gtattgtctt
    38701 taaaagaagg agtatctacg taatatgaag aaaaaaatct tagcgtcact tttattaagt
    38761 acagtaatgg tttctcaagt agctgtttta acaactgcgc atgcagaaac gactgatgac
    38821 aaaattgctg ctcaagataa taaaattagt aacttaacag cacaacaaca agaagcccaa
    38881 aaacaagttg accaaattca ggagcaagta tcagctattc aagctgagca gtctaacttg
    38941 caagctgaaa atgatagatt acaagcagaa tctaagaaac tcgagggtga gattacagaa
    39001 ctttctaaaa acattgtttc tcgtaaccaa tcgttggaaa aacaagctcg tagtgctcaa
    39061 acaaatggag ccgtaactag ctatatcaat accattgtaa actcaaaatc aattacagaa
    39121 gctatttcac gtgttgctgc aatgagtgaa atcgtatctg caaacaacaa aatgttagaa
    39181 caacaaaagg cagataaaaa agctatttct gaaaaacaag tagcaaataa tgatgctatc
    39241 aatactgtaa ttgctaatca acaaaaattg gctgatgatg ctcaagcgtt gactacgaaa
    39301 caggcagaac taaaagctgc tgaattaagt cttgctgctg agaaagcgac agctgaaggg
    39361 gaaaaagcaa gtctattaga gcaaaaagca gcagctgagg cagaggctcg tgcagctgcg
    39421 gtagcagaag cagcttataa agaaaaacga gctagccaac aacaatcagt acttgcttca
    39481 gcaaacacta acttaacagc tcaagtgcaa gcagtatctg aatctgcagc agcacctgtc
    39541 cgtgcaaaag ttcgtccaac atacagtaca aacgcttcaa gttatccaat tggagaatgt
    39601 acatggggag taaaaacatt ggcaccttgg gctggagact actggggtaa tggagcacag
    39661 tgggctacaa gtgcagcagc agcaggtttc cgtacaggtt caacacctca agttggagca
    39721 attgcatgtt ggaatgatgg tggatatggt cacgtagcgg ttgttacagc tgttgaatca
    39781 acaacacgta tccaagtatc agaatcaaat tatgcaggta atcgtacaat tggaaatcac
    39841 cgtggatggt tcaatccaac aacaacttct gaaggttttg ttacatatat ttatgcagat
    39901 taatttacag agggactcga atagagccct cttttcaggt tttaccgtga caatccctat
    39961 taaaaattat atcaaaatag cttgaaaata ttggaaaagt atggtaaaat gaaaattgtc
    40021 gtgtgaacga taatactcat tcttgatgaa ttgtgaagca gttgcccttg ggtcgttttg
    40081 cgagttgaag tcaagaagag gaaaaaaaca aaaaggagaa atactcatgg cagtaatttc
    40141 aatgaaacaa cttcttgagg ctggtgtaca ctttggtcac caaactcgtc gctggaatcc
    40201 taagatggct aagtacatct ttactgaacg taacggaatc cacgttatcg acttgcaaca
    40261 aactgtaaaa tacgctgacc aagcatacga cttcatgcgt gatgcagcag ctaacgatgc
    40321 agttgtattg ttcgttggta ctaagaaaca agcagctgat gcagttgctg aagaagcagt
    40381 acgttcaggt caatacttca tcaaccaccg ttggttgggt ggaactctta caaactgggg
    40441 aacaatccaa aaacgtatcg ctcgtttgaa agaaattaaa cgtatggaag aagatggaac
    40501 tttcgaagtt cttcctaaga aagaagttgc acttcttaac aaacaacgtg cgcgtcttga
    40561 aaaattcttg ggcggtatcg aagatatgcc tcgtatccca gatgtgatgt acgtagttga
    40621 cccacataaa gagcaaatcg ctgttaaaga agctaaaaaa ttgggaatcc cagttgtagc
    40681 gatggttgac accaatactg atccagatga tatcgatgta atcatcccag ctaacgatga
    40741 cgctatccgt gctgttaaat tgatcacagc taaattggct gacgctatta tcgaaggacg
    40801 tcaaggtgag gatgcagtag cagttgaagc agaatttgca gcttcagaaa ctcaagcaga
    40861 ttcaattgaa gaaatcgttg aagttgtaga aggtgacaac gcttaattta tacaaatagt
    40921 aattacctag gagggcgggg cttagcccgg ctctcctatt ttcaaaaaat ataggagaat
    40981 taaaatggca gaaattacag ctaaacttgt aaaagagttg cgtgaaaaat ctggtgccgg
    41041 tgttatggac gctaaaaaag cgcttgtaga aacagacggt gacatcgaaa aagcgattga
    41101 attgcttcgt gaaaaaggta tggctaaggc agctaagaaa gctgaccgtg ttgctgcaga
    41161 aggtttgact ggtgtttatg ttaacggtaa tgttgcagca gttattgaag taaacgctga
    41221 aactgacttc gttgcaaaaa acgctcaatt cgttgaattg gtaaatacta cagctaaagt
    41281 cattgctgaa ggaaaacctg ctaacaatga agaagctctt gctttgataa tgccttcagg
    41341 tgaaactctt gaagctgcat acgtatctgc aacagcaact atcggagaga aaatctcatt
    41401 ccgtcgcttt gcattgattg aaaaaacaga cgcacaacac tttggagcat accaacataa
    41461 cggtggacgt atcggtgtta tttcagttgt tgaaggtgga gacgaagcac ttgctaaaca
    41521 attgtcaatg cacatcgcag cgatgaaacc aacagttctt tcttacaaag aattggatga
    41581 gcaattcgtt aaagataagt tggcacaatt gaatcacgtt atcgaccaag acaacgaaag
    41641 ccgtgcaatg gttaataaac cagctcttcc acacttgaag tatggatcaa aagctcaatt
    41701 aactgatgat gttattgctc aagctgaagc tgacatcaaa gctgaattgg ctgcagaagg
    41761 caaaccagaa aaaatctggg acaaaattat tccaggtaaa atggatcgct tcatgcttga
    41821 taacactaaa gttgaccaag cttacacact tcttgcacaa gtttacatca tggatgacag
    41881 caagacagtt gaagcatacc ttgaatcagt taacgcttcg gtagttgagt ttgctcgctt
    41941 tgaagttggt gaaggtatcg agaaagctgc aaacgacttc gaagctgaag ttgcagctac
    42001 aatggcagca gccttgaata actaataata gaaaggaagc aaattgcttc cttttttgta
    42061 tagggcttag atttttcctg aaattgtttt ttccccagtc tcactcgatt agaataatta
    42121 aaacaaaaag ccctataata agggctaagg gcatttagga gactatactt caaattcata
    42181 gtaagcgcgt tcataacaag gtatctatcc taattcccca acttccagta tactagataa
    42241 aaaataatta tcggaggaaa ggtattgtac tatgataatt caactaagtg atttaggtca
    42301 agttcacctt gtt
//
LOCUS       DAVMMR010000011        41535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMMR010000011 DAVMMR010000000
VERSION     DAVMMR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.011"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..250
                     /locus_tag="VYS74_000577"
     CDS             <1..250
                     /locus_tag="VYS74_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788654.1"
                     /translation="FLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKSFQS
                     LKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(378..581)
                     /locus_tag="VYS74_000578"
     CDS             complement(378..581)
                     /locus_tag="VYS74_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3788655.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(574..1656)
                     /locus_tag="VYS74_000579"
     CDS             complement(574..1656)
                     /locus_tag="VYS74_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3788656.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1782..2564)
                     /locus_tag="VYS74_000580"
     CDS             complement(1782..2564)
                     /locus_tag="VYS74_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV3788657.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2564..3907)
                     /locus_tag="VYS74_000581"
     CDS             complement(2564..3907)
                     /locus_tag="VYS74_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV3788658.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4006..5322)
                     /locus_tag="VYS74_000582"
     CDS             complement(4006..5322)
                     /locus_tag="VYS74_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV3788659.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5515..6741
                     /locus_tag="VYS74_000583"
     CDS             5515..6741
                     /locus_tag="VYS74_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV3788660.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6940..8514
                     /locus_tag="VYS74_000584"
     CDS             6940..8514
                     /locus_tag="VYS74_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3788661.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8779..9567
                     /gene="codY"
                     /locus_tag="VYS74_000585"
     CDS             8779..9567
                     /gene="codY"
                     /locus_tag="VYS74_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV3788662.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9567..10142
                     /locus_tag="VYS74_000586"
     CDS             9567..10142
                     /locus_tag="VYS74_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3788663.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10224..10309
                     /locus_tag="VYS74_000587"
     tRNA            10224..10309
                     /locus_tag="VYS74_000587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10258..10260,aa:Leu,seq:aag)
     gene            10592..11722
                     /gene="ugpC"
                     /locus_tag="VYS74_000588"
     CDS             10592..11722
                     /gene="ugpC"
                     /locus_tag="VYS74_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV3788664.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12233..12991
                     /locus_tag="VYS74_000589"
     CDS             12233..12991
                     /locus_tag="VYS74_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3788665.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13078..13590
                     /locus_tag="VYS74_000590"
     CDS             13078..13590
                     /locus_tag="VYS74_000590"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV3788666.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13772..14716
                     /locus_tag="VYS74_000591"
     CDS             13772..14716
                     /locus_tag="VYS74_000591"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV3788667.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14725..15402
                     /locus_tag="VYS74_000592"
     CDS             14725..15402
                     /locus_tag="VYS74_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV3788668.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15500..16258
                     /locus_tag="VYS74_000593"
     CDS             15500..16258
                     /locus_tag="VYS74_000593"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV3788669.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16296..17801
                     /gene="lytC"
                     /locus_tag="VYS74_000594"
     CDS             16296..17801
                     /gene="lytC"
                     /locus_tag="VYS74_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV3788670.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18298..18816
                     /locus_tag="VYS74_000595"
     CDS             18298..18816
                     /locus_tag="VYS74_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV3788671.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18946..19452
                     /locus_tag="VYS74_000596"
     CDS             18946..19452
                     /locus_tag="VYS74_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV3788672.1"
                     /translation="MTKKIVAIWAQDKEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPGELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19452..19622
                     /locus_tag="VYS74_000597"
     CDS             19452..19622
                     /locus_tag="VYS74_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788673.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19654..20886
                     /gene="clpX"
                     /locus_tag="VYS74_000598"
     CDS             19654..20886
                     /gene="clpX"
                     /locus_tag="VYS74_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV3788674.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20895..21482
                     /locus_tag="VYS74_000599"
     CDS             20895..21482
                     /locus_tag="VYS74_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV3788675.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21493..21873
                     /locus_tag="VYS74_000600"
     CDS             21493..21873
                     /locus_tag="VYS74_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV3788676.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21925..22815
                     /gene="rapZ"
                     /locus_tag="VYS74_000601"
     CDS             21925..22815
                     /gene="rapZ"
                     /locus_tag="VYS74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV3788677.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22812..23789
                     /locus_tag="VYS74_000602"
     CDS             22812..23789
                     /locus_tag="VYS74_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV3788678.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23786..24697
                     /gene="whiA"
                     /locus_tag="VYS74_000603"
     CDS             23786..24697
                     /gene="whiA"
                     /locus_tag="VYS74_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV3788679.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24731..25696)
                     /locus_tag="VYS74_000604"
     CDS             complement(24731..25696)
                     /locus_tag="VYS74_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3788680.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25832..26689
                     /locus_tag="VYS74_000605"
     CDS             25832..26689
                     /locus_tag="VYS74_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV3788681.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26676..27455
                     /locus_tag="VYS74_000606"
     CDS             26676..27455
                     /locus_tag="VYS74_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV3788682.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27479..28831
                     /locus_tag="VYS74_000607"
     CDS             27479..28831
                     /locus_tag="VYS74_000607"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV3788683.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28905..29279
                     /locus_tag="VYS74_000608"
     CDS             28905..29279
                     /locus_tag="VYS74_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV3788684.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29281..30129
                     /locus_tag="VYS74_000609"
     CDS             29281..30129
                     /locus_tag="VYS74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3788685.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30395..31162
                     /locus_tag="VYS74_000610"
     CDS             30395..31162
                     /locus_tag="VYS74_000610"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV3788686.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31174..32358
                     /locus_tag="VYS74_000611"
     CDS             31174..32358
                     /locus_tag="VYS74_000611"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV3788687.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32355..34226
                     /locus_tag="VYS74_000612"
     CDS             32355..34226
                     /locus_tag="VYS74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3788688.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34369..35553)
                     /gene="mntE"
                     /locus_tag="VYS74_000613"
     CDS             complement(34369..35553)
                     /gene="mntE"
                     /locus_tag="VYS74_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV3788689.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35857..38553
                     /locus_tag="VYS74_000614"
     CDS             35857..38553
                     /locus_tag="VYS74_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV3788690.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38566..39357
                     /gene="yghU"
                     /locus_tag="VYS74_000615"
     CDS             38566..39357
                     /gene="yghU"
                     /locus_tag="VYS74_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV3788691.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39473..39883
                     /locus_tag="VYS74_000616"
     CDS             39473..39883
                     /locus_tag="VYS74_000616"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3788692.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39895..41490
                     /locus_tag="VYS74_000617"
     CDS             39895..41490
                     /locus_tag="VYS74_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788693.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12166 a         7786 c         9506 g        12077 t
ORIGIN      
        1 gtttcttgag ggtaagggaa ttcaagcatc catgtcacgc aagggaaaca gccaagacaa
       61 cggtatgatg gagtccttct ttgggattct gaaatcggaa atgttttacg gttatgagaa
      121 gtcgtttcag tcgcttaagc aattggaaca agctattgta gactatattg attactacaa
      181 caataaacga attaaggtaa aactaaaagg acttagtcct gtgcaataca gaactaaatc
      241 cttcggataa attatttgtc taactgtttg ggggcagtac attttaacac ttgatactat
      301 gcgttttatt gtgggaagat tttcctcatt ttctagtgaa attgaacttt ttctaacatc
      361 ttttgataga tgacttctta atcaattatc atttccattg ttgtttcttg gggtttcatc
      421 ccttgtcttt gataaaagcg gagagcaccc tcgttatcat tccagacact tagggttaga
      481 ttattgcagc caatttcttt agcataatag cagaaaaatg acaataaaat agcccctaga
      541 tattttgaaa ggaaagcata ggggctatat agtttaatca aaataaagca aatctttgct
      601 ggtgcttgta aagaggtcga agccatcctt ggtaacaaca ccgcagtctt caatacgaac
      661 accgacttta ccagggatat agatacctgg ttcaacagag aagcacatgc cttcttcgat
      721 gaccatgtcg tttccttcca tgatagatgg gaattcatgg acatccatac cgataccatg
      781 cccgagacgg tggttgaagt actcaccata accagctttt tcgatgacct cacgggcagc
      841 gcggtccact tcatgagcag tcacacctgg cttgataaag tcaagagcag cttgttgggc
      901 ttcaagagtc aagttgtaaa tatctttctt gaattggtct ggtttgccga cagcgactgt
      961 acgagtcata tctgacgcat agccattgac cagaacaccc aggtcaaaga ggagaagagc
     1021 atcattttca accttattag ctgctggaat gccgtgtgga ttcgcagcat tatcaccagt
     1081 caagaccatg gtatcaaagc tcatttcata accttcacgt ttcatggcaa agtcgatttg
     1141 tgcgatgata tctgtctcag tcttatcaag agaaatattg tcaaaaccaa catgaacagc
     1201 cttgtcagca taaagacctg caaccatcat tttttgcact tcatcagctg atttgatgag
     1261 gcgcatgcgt tggatacgag gagtgaggtt gtcaaactca gcagtctcaa aaactgtttt
     1321 caaaccatgg tatttggtca agatgagatt gtcaaactca acagcgacac gtttgaagtc
     1381 aagttgtgga agagcatgtt tgattttttg ccatggattt tcagaatcga catagcccac
     1441 tactgggaag gaaacggtgc tacttgcacg ttctacttca agagctggga caaagaggag
     1501 aggttcctga tccgctagga caaagaggaa catttggcgt tcatggggat cactgtaaaa
     1561 accagtgagg taattgattg tgacggggtc agatacgaca gcgacgtcta gtttttctga
     1621 ttcaagatat gttaggattt gttgtaattt agacatatgc taccttcttt ctacccctct
     1681 attttggcaa aaaatgagag aaaatgcaag ggagaagggt aaaagaaaag acaaaaaact
     1741 ccgacaagat aacggagttc tttgatatca ttttcctttg tttaagaaaa gtcagccttg
     1801 cttttgacgt cactgtactc ttcagcgatt tcttggctga gaatgtcctc ataggcaggg
     1861 agttggatgt cctgaccata tttcttcttg agggcagtag taactaggcg ataagccaga
     1921 ttggcattac gagagaggat aggcccgtgg aagtaggaac caaagacatt cttataatga
     1981 accccttcac cgaccttttc ttcgttgttt ccatttccat agacaacctg tcccagcggt
     2041 ttttggtcat cagagaggaa ggtacgacct tggtgatttt caaatccata gtaggtttca
     2101 tcgaaatctt cattgtgaat cttgatgtca ccgataaaac ggttattggt ctggttgagc
     2161 gtgtagtgtc ccatgacccc tagcccttcg atacgttttc ctgaagcttc aacataatat
     2221 tgacccaata gttggaaacc accgcagata gccagaacta caccgtcgtt ttggatgtag
     2281 ttgtcaatgc tctctttttt agcaggtagg tcgtctgcaa tgatactttg ttcaaagtct
     2341 tgaccaccac cgaaaaaggc gatgtcgtag tgattttcat caaagtcatc atggagagaa
     2401 acgatgtcaa cggtcacatg ggctcccagt ttttcagcca catacttgag catgaggatg
     2461 tttccattgt ccccgtaggt attcatgaga tttccgtaga ggtgggcaat gttgagctga
     2521 taggggtaat tgctatcttt tgaggaaagt gaagtataaa ccattagttc atctcctttc
     2581 taacaatctg acgactagcc agcagttcac gaaattccag catggcagta taagttgcca
     2641 gaatataggc atgcttgcag tcttgattct caatggtctt gagaacttgc tccagattac
     2701 tcgtttcagt gattttctca gctggatagc cagtcactcg gaggcgacga gcgatttcag
     2761 aatgacgaac accgccagcg ttgatttcag gaatgtccat gtcagtgatt tgctcaaagt
     2821 ctgcatccca gatccagcta gtgtcaattc catctgcata gttggcatta aggaggacag
     2881 ataggctaaa tggataaggt gctagtttga tcatttcgat agcttgggtt gcaccgactg
     2941 gatttttaat caagacaagg gtacattcct tgtcaccgat atgaaaggtt tcttggcgtc
     3001 caaagacagc acggctcttg tcaaatccct gtttgatgag ttgggaatct gcgccgagga
     3061 aacgggcgat ggccacagca gctagggcgt tatagatatt atagagcccg ccgatttgga
     3121 taccgtattc ttggccgtct atgacaaagc gagagcgatt gttggtcaac tcaaccagtt
     3181 ttgtcaaacg atagtcgaga tcaggacgtt tacatccaca accttcacag atataggcac
     3241 ccaagtttgc ataggtatta tgctcatatt tgaggatgcc ttggcagtca ggacagagaa
     3301 tcccttcggt attgtagtga gcaagttggg ctggtccttt ttccaagtca aaaccaaaat
     3361 actctatagg gtttggaata gttggcttgt agaaaagtgg actgtctccg ttaaggagaa
     3421 cagtagcagt tggaactttt cgaatggcat ccaatatcat gttataggta gtatagattt
     3481 caccgaaacg gtccatctgg tcacggaaga tattagtaat gacaaaaaga ctaggctgga
     3541 tatagtcaca gatacgagat agactggctt cgtcaatttc gaggacggca atatttttcc
     3601 cagtttttga agatttggct gttaggaagg ttgttgcaat ccctgtaatc atgttggcac
     3661 cgcttgggtt ggttagaact tgaccataaa cctcttttaa aatgccgaca gtgagggcag
     3721 ttgtcagggt ttttccattt gttccagtga caacgacaat ctcgtagttc ttagctaggc
     3781 tttgtaaaat atctttatca aattgaaggg cgactttccc tgggagcgta cttccacgtc
     3841 caagacggct taaaacgaag tgggaagaac gcccagcaag aaggcccaaa gtagttttta
     3901 agttcatgtt tctattatat cacaaaagag ggaaaagaca gatttggtgt ttcgtagaag
     3961 taaaaacagc ccaaagaggg ctgtcagtta agatgagatt tcaacttaaa tcgcaaacaa
     4021 gtcattcaag ttctcagcca aggttgggtg agtgaagatt tgttttgtga agtaagtgta
     4081 aggaatcttg ttgtccatag caacagtaat gatgttgatg atttcttgag aaccttctga
     4141 gaagatgctt gcaccaagaa tttcttttgt ttcagtatta acaacagctt tgaaagctcc
     4201 gcgaaggtct ccatttacgt gaccacgagg catggctgca acagggattt ctttcactgc
     4261 gtatggaagt ttcaaatcag ctgcttggct ttcagtcaaa ccaacttgtg aaagtgcagg
     4321 tgtgatgaac atagtatttg gcacattgag acggtcctca agtgtgtagc tgccatctcc
     4381 agcaaggtag ctgtaaacaa cacggaagtc atcaagtgaa atgtaagtaa attgaaggcc
     4441 accgttgaca tctccaactg caaagacacc aggaacgttt gtttgacagt gtttgtctac
     4501 tttaatagca ccacgttcag ttagttcaat atctgtattt tcaagttgaa gtggttctac
     4561 atttggtttg cgtccagttg cgtagagaag ggcgtcgaaa cggtaagttt cgtcttcagt
     4621 tacgacaagc acttggtcac catcgttttt gatttcagta gtatggatat tttgaagcaa
     4681 ttcaatgcca tcttcttcca tgtattgttt agcaagagct gcgatggaag gttctgcacg
     4741 aggtaggaat gtatccaagg catctaggac tgtgaccttg cttccaagtt tgttgtaaag
     4801 gccggcaaat tcaagaccga tatttccgcc accaaggatt ccaagttttt caggtaattt
     4861 gtccaagctt tggatacctg ttgagtcaaa gatgtttttg cttgtagcaa gtccaggaat
     4921 tggcaagacg tttgaaacag caccagtgtt gatgacgatt gtttcagcag tcagttcttt
     4981 cttttcatca ccagcttgga tttcgatgac tttatttgaa aggaagtgag cttccgcatc
     5041 aaagatatct acgcctgtac cagcaacagt cgcatagttt ttaccgttga ggcgaccagt
     5101 gatcgtgttt ttagtagcaa tcacttcttc aaaagacaag tccttttcag cagcaactag
     5161 caaggtttta gttgggatac aaccaatgtt gatacaagtt ccaccgtaca tagccttgct
     5221 gcgttcaacg agggcaactt ttttgccagc tgaagccaat ttacctgcta gtgttttacc
     5281 agctttacca aatccaataa caattaaatc gtatgttaac atttatcttc ctcctattcg
     5341 aatttcattc tagcacaaga aaaaaacttt tgcacaattc tgctacggaa aagacagaaa
     5401 aagataaaaa cgctttccag acaaaaaact agtaattttt tgacaagctt gataaactaa
     5461 aatcctatct atttttatat aagaaagaat aaatgttcga aaataggagg ttttatgaaa
     5521 tcgaatcgtt atattattgc ctttgctggg gtcattttac acttaatgct gggttcgact
     5581 tatgcttgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga tcaggcttct
     5641 gttgccttcg cctttagtct agcaatcttt tgtttgggct tatcggctgc atttatgggg
     5701 cgtttggtag aaaaatttgg tccgaaagtc atggggagtc tatctgcttt tctatacgca
     5761 ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt gttgtatcta
     5821 gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc tgtgtcaacg
     5881 attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat tatggggttt
     5941 ggttttgctt ctttattgac tagtcccata gcgcaacacc tcatcgcagg ggtagggatt
     6001 gtagaaactt tttatatttt aggagcaagt tactttatta tcatgctcct agcttcacaa
     6061 ttcattaagc gtccaaatga gcaagagctt gcaattttat cttcttcagg gaaagaaaaa
     6121 acagcctctt tgacgcaagg aatggctgca aatcaggctc taaaaagcaa tcggttttat
     6181 atgctttgga ttattttctt tatcaacata gcttgtggtt taggcttaat ttcagcggca
     6241 tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt aatggtgggt
     6301 gttttgggga ttttcaatgg atttggtcgc ttgctctggg cgagtttgtc tgactatatc
     6361 ggtcgccctc taacctttag tatattactg cttgtcaatc ttttcttttc tctctcactt
     6421 tggctcttta cagattccgt tttatttgta gttgctatgt ctattttgat gacttgctat
     6481 ggagctggtt tttctttgat tccagcttat ctcagtgata tttttggaac caaggaattg
     6541 gccgctctgc atggttatat tttaacagct tgggcaatgg ctggtttagc gggacctatt
     6601 ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt cgtttttctc
     6661 attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat caaaaaagaa
     6721 agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc tctggtcttg
     6781 tgttatacta gataggttgc aaagaaaaca gtacttttct tttgtggaaa agaagcaaca
     6841 cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa actaagccct
     6901 atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa tgaattaaac
     6961 ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc tagtcctatc
     7021 caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca agctcagact
     7081 ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg tatagaagaa
     7141 gcgactattc aagccttggt catcgctcca actcgtgaac tagctgtcca aagtcaagaa
     7201 gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata tggcggatca
     7261 agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt gggaactcca
     7321 ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaagacat tgaaaccctt
     7381 atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat cgaagccatt
     7441 atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat gccagatgcc
     7501 atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat tgcggctaag
     7561 gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca agaaaaattt
     7621 gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt atttggtcgt
     7681 accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa
     7741 ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat
     7801 ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga tatttcaggt
     7861 gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt tcaccgtatc
     7921 ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgttgc tccaaacgaa
     7981 atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg tctcaaacct
     8041 gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt
     8101 gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa
     8161 ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct gacagtccaa
     8221 gacccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc gtttaaacca
     8281 tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg tggggacgac
     8341 cgtcgagagc gtgatcgtcg tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt
     8401 ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa accacgcaat
     8461 actttaagcg aaaagcaaac aggctttgtt attcgtaaca aaggtgataa ataagaaaaa
     8521 gcggttcaaa atgaatcgct tttttatata aggagaccga atgtgaaaaa gacaaataaa
     8581 attattatat tatcttttat aatattatga tacacaaaaa taggcgactt gtcaatagaa
     8641 aaggaacaat ttaattaaaa atattctggt ttttaattaa attgtaagtc aatgcaagtc
     8701 aataaggaat tttcagataa ttattgaaaa agagacgttt ttatgttata atgatagcga
     8761 ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt
     8821 ttgaaacgct cagaggagca gttgcaggat gagctccctt acaacgctat tacgcgtcag
     8881 ttagcggata ttattcattg caatgcctgc attatcaata gtaagggacg tctgcttggc
     8941 tattttatgc gttataaaac aaatacagat cgcgtagagc aattcttcca aactaagatt
     9001 ttcccagatg actacgttca aggggctaat atgatttacg aaacagaagc aaacttgcct
     9061 gttgagcatg atatgagtat tttccctgtt gagagtagag atgattttcc agatggcttg
     9121 acgactattg caccgattca tgtatcgggg attcgccttg gttctttgat tatttggcgt
     9181 aatgataaaa aattcgaaga tgaggacttg gttcttgttg agattgccag taccgttgtt
     9241 gggattcagc ttcttaactt ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact
     9301 gctgtcacca tggcggtcaa taccctttct tactccgaac tccgtgctgt ttcagcaatt
     9361 ttaggggaat taaatggaaa tgaagggaag ttgactgcgt cagtgattgc agatcgtatc
     9421 ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt
     9481 gaaagtcgct cacttggaat gaagggaacc tatcttaagg tcttgatttc agatattttt
     9541 gaagaagtga agaaaagaga ttactaatga caaaggcttt aatttcgatt gattatacag
     9601 aagattttgt tgctgatagt gggaagctga cagcaggtgc tccagctcag gcgatttcgg
     9661 atgccatcag caaggtaact cgattagctt ttgaacgagg agattatatc ttctttacta
     9721 ttgatgctca tgaagaaaac gattgtttcc atccggaaag taagttattt cctcctcata
     9781 atctgattgg gacgagtgga cggaatttat atggagattt ggggatcttt tatcaagagc
     9841 atggttcaga cagtcgtgtc ttttggatgg ataaacgcca ttactcagct ttttcaggga
     9901 ctgacctaga tattcgtttg agagagcgta gagtgtctac cgttatctta acaggtgtct
     9961 tgacggatat ctgtgtccta catacagcta tagatgccta taatctagga tatgacatcg
    10021 agattgttaa accagctgtt gcttccatct ggcctgaaaa tcatcaattt gccctaggtc
    10081 atttcaaaaa tacactcgga gctaagttag tagatgaaaa tctaaatgaa ctttctgagt
    10141 aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt
    10201 gatatactag taaagtaatc gatgcgggga tggcggaatt ggcagacgcg caggactaag
    10261 gatcctgtga ccgctttagg tcgtgagggt tcaagtccct ctctccgcat aggaaaagag
    10321 ttagctaaag ctagctcttt tgtatttctc taaaaaatta gaatccagtc aaatgtagtg
    10381 gagaatcagc gaaaatatgg gaaaactgat ccttgtttct gcttttgtca tagaagaata
    10441 ataaagatta tcttcttcaa attctccgat attctctaaa gttttgtgca agttgcacag
    10501 aactttttta tttttttggt catcttgcca tagaaatata aagcgttttc atctataata
    10561 taattatcaa aagacaaaag gagttcacct catggtagaa ttgaatctta aaaatattta
    10621 caaaaaatat ccaaacagcg aacactattc agttgaagat ttcaacttga acatcaaaga
    10681 taaagaattt atcgttttcg taggaccttc aggatgtggt aaatcaacta cactccgtat
    10741 gattgctggt cttgaagaca tcacagaagg tactgcatct atcgatggcg tggttgtcaa
    10801 cgacgtagct ccaaaagacc gtgatatcgc catggtattc caaaactacg ctctttaccc
    10861 acacatgact gtttatgaca acatggcttt cggtttgaaa ttgcgtaaat acagcaaaga
    10921 agacattaac aaacgtgttc aagaagcagc tgaaatactt ggattgaaag aattcttgga
    10981 acgtaaaccg gctgaccttt caggtggtca acgtcaacgt gttgccatgg ggcgtgcgat
    11041 tgtccgtgat gcgaaagtat tcttgatgga cgaacctttg tcaaacttgg atgccaaact
    11101 tcgtgtatca atgcgtgctg aaatcgctaa aattcaccgt cgtatcggag ctacaactat
    11161 ctatgtaact cacgaccaaa cagaagcgat gacacttgca gaccgtatcg ttattatgtc
    11221 agctactaag aaccctgctg gtacaggtac tatcggacgt gtagaacaaa tcggtactcc
    11281 tcaagaagtt tacaaaaatc cagttaacaa attcgttgca ggattcatcg gaagcccagc
    11341 tatgaacttc atcaacgtga aattggttgg tagcgaaatt gtttctgacg gtttccgttt
    11401 gaaagtgcca gaaggagcat tgaaagttct tcgtgaaaaa ggctacgaag gaaaagaatt
    11461 gatctttggt atccgtccag aagacgtgaa tgcagaacct gctttccttg aaacattccc
    11521 agactgtgtt gtaaaagcga ctatctctgt atcagaactg cttggttcag aatctcacct
    11581 ttactgccaa gttggtaaag acgagtttgt tgcaaaagtt gatgctcgtg actacttgca
    11641 aacaggtgca acagttgagc ttggatttga cttgaacaaa gcacacttct tcgatgtaga
    11701 aactgaaaaa acaatctact aagaataaat aaaattcaaa gcactacaag aaaagatatc
    11761 tctttatcaa ttgtagtgga gagatatcag ttaatctagg gagagaaaca aaatgcttct
    11821 ctcctttttg ctagggaagt catattatac atctatattg tgatgctctt tatactcttc
    11881 gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtac gtatgattac tgacttcgtc
    11941 agttttatct gcaacctcaa agctgtactt tgagtagctt acggctagtt tcctagtttg
    12001 ctctttgatt tccattgagt attatttgtg ggtaccatct acaagtgaag ctatatgcgt
    12061 aaactacgtg agcaattgaa ttcgaactag agaggtaata ataaatttat gctatagtta
    12121 tggtgacttg tatgcttttg attctagttt atcaaataat agattagaat tgtcagataa
    12181 tatcattttg tgtcattttg tgttataatg aagaaaaaac agaggtgttc aaatgtcaga
    12241 agcaggtcat aagtttttag caaaattggg gaaaaaacgc ttacgtccag gtggaaagcg
    12301 tgccacagat tggttaattg cagaaggagg attttcaaaa gaaaagagaa tactagaggt
    12361 tgcgtgtaat aggggaacta cagcaattga gttggcacag cgttttggtt gcaagataac
    12421 tgctgttgat atggatgctc aagctttaga agtggctaaa aaatctgctg gaacggcagg
    12481 tgttgctcat ttaatcagtt ttgaaagagc aaatgcaatg aaacttcctt atcaagatgc
    12541 tagttttgat attgttataa atgaagctat gctgactatg caagccgatc aagctaagaa
    12601 aaaatgtgta atggaatatc taagggtatt aaaacctgga ggtcttctct tgacacatga
    12661 tgtgcttctt aaggaagcta aagagtctat cagacaggaa ttatcacaag caattcatgt
    12721 aaatgtaggt cctttaactc aagatggttg ggaacaggtg atgatagaat caggttattg
    12781 tgatgtgaaa gtattgactg gtgaaatgac attaatgaaa ttatcgggta tgatttatga
    12841 cgaaggttgg ctaggaactt tgaaaatttg tgtaaatgct tgtaaaaagg agaatagaaa
    12901 gcagttttta actatgtata aaatgtttgc taagaataaa cagaaattgg gctttattgc
    12961 gatggctagt tataaatcgt caaaacgtta gataattatt gaagttaact tttccttttt
    13021 tctttcttaa aaaatatgct ataatagaga gtaaaaaact ttgaaagaaa gaaaaagatg
    13081 aatttaaaag attacattgc aacaattgaa aattatccaa aggaaggcat taccttccgt
    13141 gatattagtc ctttgatggc tgatggaaat gcttatagct acgctgttcg tgaaatcgtt
    13201 cagtatgcta ctgacaagaa agtcgacatg atcgtgggac ctgaagctcg tggatttatc
    13261 gtgggttgtc cagttgcctt tgagttggga attggttttg cgcctgttcg taagccaggt
    13321 aaattgccac gcgaagttat ttctgctgac tatgaaaaag agtacggtgt cgataccttg
    13381 actatgcacg cggatgccat taagccaggt caacgtgttc ttattgtaga tgaccttttg
    13441 gcgacaggtg gaactgttaa ggcaactatc gagatgattg aaaaacttgg tggtgttatg
    13501 gcaggttgtg ccttccttgt tgaattggat gaattgaacg gccgtgaaaa aattggtgac
    13561 tacgattaca aagttcttat gcattattaa tgaaaacagt ccctagggct gttttctcta
    13621 cactaggata taaaaataga ctataactag ttagagaaaa actataattg aaaactatat
    13681 cttcttacag tataataaaa ggactaagtg tttgagattt gtcttcaaac atatgcaatt
    13741 attcctgaaa gagtacagtt aggagagggt tatgccgatt cgaattgata aaaaattgcc
    13801 agctgttgag attttacgga cagagaatat ctttgtcatg gatgatcaac gtgctgccca
    13861 ccaagatatc cgtcctttga agattttaat tttaaatctc atgccacaga aaatggtcac
    13921 agagacccag ttgttgcgcc acttggctaa tacaccccta caactggata ttgattttct
    13981 ctatatggag agccaccgtt ctaaaacaac tcgttcagag cacatggaga ccttctataa
    14041 aacttttcct gaagtcaagg atgagtattt tgatgggatg atcatcacgg gtgctccagt
    14101 tgagcattta ccatttgagg aagtggacta ttgggaggaa tttagacaga tgcttgagtg
    14161 gtctaagact catgtctatt cgacccttca tatctgttgg ggggctcagg ctgggcttta
    14221 tctgcgctat ggtgtagaaa aataccagat ggacagtaag ctatcaggta tttatcctca
    14281 ggacacccta aaagagggtc accttctatt tagaggcttt gatgatagct atgtatcccc
    14341 tcattcacgg cacacggaga tttctaagga agaggtctta aacaagacca atctcgagat
    14401 tttatcagaa ggacctcagg ttggggtttc tattttggcc agtcgtgatt tacgagaaat
    14461 ttatagtttt ggtcatttgg agtatgaccg tgataccttg gcaaaagagt attttcgaga
    14521 tcgtgatgca ggtttcgacc cacatattcc agaaaattac tttaaagatg atgatgttaa
    14581 tcaggtacct tgtctttgtt ggtcttcatc tgcagccctc tttttcagta attgggtaaa
    14641 ccatgcggtc tatcaggaga cgccttttga ttggagaaag atagaagatg atgcatctgc
    14701 atatgggtat ttataagagg aattatgaca tatttagacg cttttaaatc aggtaccttg
    14761 gttttaccga gtgccctgct cttgcatttt aaggaactct ttccttctag cgacgatttt
    14821 ctggtttggc aatttttcta tttgcaaaat acgacaggct tagaagaaat gtcgccaagc
    14881 cagattgctg aaaggattgg caaggaaatt tcggatgtca accagtccat ttctaatctg
    14941 acggaaaggg gactgctcca gtatcgtact atcgaattaa atggcgaaat tgaattgttc
    15001 tttgatgcta gtttggcctt ggaacgtttg gatgacctgt ttggagcagt tcgttcaagt
    15061 tcagaccagc taacacctca aaaccagctc aaggatttgg tggaaacctt ccagcaggag
    15121 ttgggacgat tgttgacgcc ttttgagatt gaggatttga ccaagacact aaaggaagat
    15181 ggaaccagtg ctgacttgat taaggaggct cttcgtgaag ctgttttgaa tggaaaacca
    15241 aactggaagt acattcaggc aattttgaga aactggcgcc atgaaggaat caagagtgtg
    15301 gctcaaattg aggccaagag agcagaaaga gaagcaagca atcctcagtt gacacaggta
    15361 tctgcagatt tcagaaatgc catggatctc tggaaggatt aatccatgca agtaggcttg
    15421 aaatccgagt aagatttgca agctgtgtat aattgtgata gaataaatag aaaataaatt
    15481 gaaaaaagag gtatgtgaaa tgtcacgtaa accatttatc gctggtaact ggaaaatgaa
    15541 caaaaatcca gaagaagcta aagcattcgt tgaagcagtt gcatcaaaac ttccttcatc
    15601 agatcttgtt gaagcaggta tcgctgctcc agctcttgat ttgacaactg ttcttgctgt
    15661 tgcaaaaggc tcgaacctta aagttgctgc tcaaaactgc tactttgaaa atgcaggtgc
    15721 tttcactggt gaaactagcc cacaagtttt gaaagaaatc ggtactgatt acgttgttat
    15781 cggtcactca gaacgccgtg actacttcca tgaaactgac gaagatatca acaaaaaagc
    15841 aaaagcaatc tttgcgaacg gtatgcttcc aatcatctgt tgtggtgaat cacttgaaac
    15901 ttacgaagct ggtaaagctg ctgaattcgt aggtgctcaa gtatctgctg cattggctgg
    15961 attgactgct gaacaagttg ctgcctcagt tatcgcttat gagccaatct gggctatcgg
    16021 tactggtaaa tcagcttcac aagacgatgc acaaaaaatg tgtaaagttg ttcgtgacgt
    16081 tgtagctgct gactttggtc aagaagtcgc agacaaagtt cgtgttcaat acggtggttc
    16141 tgttaaacct gaaaatgttg cttcatacat ggcttgccca gacgttgacg gtgcccttgt
    16201 aggtggtgcg tcacttgaag ctgaaagctt cttggctttg cttgactttg taaaataatc
    16261 agtaagtagc aaaagctagg tggaacagca ttcagatgtc tgttacattt tttataggag
    16321 aggaaagatt gaaaataaaa attggattag caagtatctg tttactaggc ttggcaacta
    16381 gtcatgtcgc tgcaaatgaa actgaagtag caaaaacttc gcaggataca acgacagctt
    16441 caagtagttc agagcaaaat cagtcttcta ataaaacgca aacgagcaca gaagtacaga
    16501 ctaatgctgc tgcccactgg gatggggatt attatgtaaa ggatgatggc tctaaagctc
    16561 aaagtgaatg gatttttgac aactactata aggcctggtt ttatattaat tcagatggtc
    16621 gttactcgca gaatgaatgg catggaaatt actacctgaa atcaggtgga tatatggccc
    16681 aaaacgagtg gatctatgac agtaattaca agagttggtt ttatctcaag tcagatgggg
    16741 cttatgctca tcaagaatgg caattgattg gaaataagtg gtactacttc aagaagtggg
    16801 gttacatggc taaaagccaa tggcaaggaa gttatttctt gaatggtcaa ggagctatga
    16861 tgcaaaatga atggctctat gatccagcct attctgctta tttttatcta aaatccgatg
    16921 gaacttatgc taaccaagag tggcaaaaag tgggcggcaa atggtactat ttcaagaagt
    16981 ggggctatat ggctcggaat gagtggcaag gcaactacta tttgactgga agtggtgcca
    17041 tggcgactga cgaagtgatt atggatggta ctcgctatat ctttgcggcc tctggtgagc
    17101 tcaaagaaaa gaaagatttg aatgtcggct gggttcacag agatggtaaa cgctatttct
    17161 ttaataatag agaagaacaa gtgggaaccg aacatgctaa gaaaatcatt gatattagtg
    17221 agcacaatgg tcgtatcaat gattggaaaa aggttattga tgagaacgag gtggatggtg
    17281 tcattgttcg tctaggttat agcggtaaag aagacaagga attggcgcat aacattaagg
    17341 agttaaaccg tctgggaatt ccttatggtg tctatctcta tacctatgct gaaaatgaga
    17401 ccgatgctga gaatgacgct aaacagacca ttgaacttat aaagaaatac aatatgaacc
    17461 tgtcttaccc tatctattat gatgttgaga actgggaata tgtaaataag agcaagagag
    17521 ctccaagtga tacagacact tgggttaaaa tcatcaacaa gtacatggac acgatgaagc
    17581 aggcgggtta tcaaaatgtg tatgtctata gctatcgtag tttattacag acgcgtttaa
    17641 aacacccaga tattttaaaa catgtaaact gggtagcggc ctatacgaat gctttagaat
    17701 gggaaaaccc ttattattca ggagaaaaag gttggcaata tacctcttct gaatacatga
    17761 aaggaatcca agggcgcgta gatgtcagcg tttggtatta agcgatgatt tgaaagaggg
    17821 atgtgatagt agcaccctct ttttctttgt tttatgatag ttcatcctcg agtaaattca
    17881 agatcttgct cggaaatgaa gcttatatag tagattgaat atagataaag accttgtgat
    17941 tggtaaaaca ttttagaaat tcatttacct ttcctaatcg acttggtttc atcttatttc
    18001 aatctattat agtattgggg aatttcttca aaccacatca gcttggtcag tcctacctgc
    18061 gacctcaaaa cttgtgcttt ggtcaagctg ggtttagttt cctagtttgc tgatggattt
    18121 ccattgacta taagcatcca accctctttt ttgtcttcta aagaattctt aaattatcag
    18181 tctattgcaa cttttctcat ataagttctt tgtcttgcta ttggttttcc ttagtagtat
    18241 actaaggtag taatcattaa gaagtggtta caaaaaataa tgaatgaggt aaagaaaatg
    18301 gtagaattga aaaaagaagc agtaaaagac gtaacatcat tgacaaaagc agcgccagta
    18361 gcattggcaa aaacaaagga agtcttgaac caagctgttg ctgatttgta tgtagctcac
    18421 gttgctttgc accaagtgca ctggtatatg catggtcgtg gtttccttgt atggcatcca
    18481 aaaatggatg agtacatgga agctcttgac ggtcaattgg atgaaatcag cgaacgcttg
    18541 attacactcg gtggaagccc attctctaca ttgacagagt tccttcaaaa tagtgaaatc
    18601 gaagaagaag ctggtgaata ccgtaatgtt gaagaaagct tggaacgtgt tcttgttatc
    18661 taccgttact tgtcagaact tttccaaaaa ggtttggatg tcactgatga agaaggtgac
    18721 gatgtgacaa acggtatctt tgcaggcgct aaaactgaaa cagataaaac aatttggatg
    18781 cttgcagccg aacttggaca agcacctggt ttgtaagaaa taaggaaaca tagataaatc
    18841 tgtaggaaat ttcttacaga ttttttctat tttgtaagct attccaaacc agtctttttt
    18901 tgagtttttt gataaaatag tactatcagt gaaaaggatg gaagcatgac taagaaaatc
    18961 gtagctattt gggcccagga taaagagggt gtgattggta aggaaaatcg tctgccttgg
    19021 catttgccag cagaattgca gcactttaaa gaaacaactc tgaatcatgc tatcttgatg
    19081 gggcgtgtga cctttgatgg gatggggcgt cgcttgcttc caaaacggga aaccctgatt
    19141 ttgacgcgta atccggaaga aaagatagat ggggttgcta cttttcagga cgtccagtct
    19201 gttcttgatt ggtatcaggc tcaagaaaag aatctctaca ttatcggtgg gaagcaaatt
    19261 tttcaggctt ttgaagctta ccttgatgaa gtgattgtca ctcacattca tgctcgggtg
    19321 gaaggagata cctatttccc tggagagctt gacttgtctc tttttgagac agtttcaagc
    19381 aaattttacg ccaaagatga gaagaatcct tatgatttta ccatccaata ccgcaagaga
    19441 aaggaagtct aatggaacgg agtttatttg gtctattcac ggcttttctg tgtttcatct
    19501 gctttctggc aggagcacaa gcttttcgta aaaagcgtta cgggctttct atcctccttt
    19561 ggttaaatgc ctttaccaat ctggtcaata gtattcatgc tttttacatg accttatttt
    19621 agatagaatg acagtataga atggaaggaa atcatgtcta caaatagaaa aaatgatatg
    19681 atggtttatt gctcattttg tggcaaaaac caagaagaag tacaaaaaat aattgctggc
    19741 aacaatgctt ttatttgtaa tgaatgcgtg gagttagctc aggaaatcat tcgagaagaa
    19801 ttggttgagg aagtcttggc agacttgtct gaggtgccaa aaccaattga actcctccat
    19861 atcttgaacc actatgtaat tggtcaagat cgtgccaagc gtgccttggc agtggcggtt
    19921 tataatcact acaaacgcat caatttccac gatacacgcg aagagtcaga agatgtggat
    19981 ttgcagaagt caaacatttt gatgattggc ccaactggtt cagggaaaac tttccttgcc
    20041 cagaccttgg ctaagagctt gaatgtgcct tttgctattg cggatgcgac agctctgacg
    20101 gaggctggtt atgtgggtga ggatgtggaa aatatcctcc tcaaactctt gcaggttgct
    20161 gactttaaca tcgaacgtgc agagcgtggc attatctatg tggatgaaat tgacaagatt
    20221 gccaagaaga gtgagaatgt gtctatcaca cgtgatgttt ctggtgaagg ggtgcaacaa
    20281 gcccttctca agattatcga gggaactgtt gctagcgtgc cgcctcaagg tggacgcaaa
    20341 catccacaac aagagatgat tcaagtggat acaaaaaata tcctcttcat cgtgggtggt
    20401 gcttttgatg gtattgaaga aattgtcaaa caacgtctgg gtgaaaaagt catcggattt
    20461 ggtcaaaaca ataaggcgat tgacgaaaac agctcataca tgcaagaaat catcgctgaa
    20521 gacattcaaa aatttggtat tatccctgag ttgattggac gcttgcctgt ttttgcggct
    20581 cttgagcaat tgaccgttga tgacttggtt cgcatcttga aagagccaag aaatgccttg
    20641 gtgaaacaat accaaacctt gctttcttat gatgatgttg agttggaatt tgacgacgaa
    20701 gcccttcaag agattgctaa taaagcaatc gaacggaaga caggggcgcg tggacttcgc
    20761 tccattatcg aagaaaccat gctagatgtt atgtttgagg tgccgagtca ggaaaatgtg
    20821 aaattggttc gcatcactaa agaaactgtt gatggaacgg ataaaccgat cctagaaaca
    20881 gcctagaggt gactatggaa cttaatacac acaatgctga aatcttgctc agtgcagcta
    20941 ataaatccca ctatccgcag gatgaactgc cagagattgc cctagcaggg cgttcaaatg
    21001 ttggtaaatc cagctttatc aacactatgt tgaaccgtaa gaatctcgcc cgtacatcag
    21061 gaaaacctgg taaaacccag ctcctgaact tttttaacat tgatgacaag atgcgctttg
    21121 tggatgtgcc tggttatggc tatgctcgtg tttctaaaaa ggaacgtgaa aagtgggggt
    21181 gcatgattga ggagtactta acgactcggg aaaatctccg tgcggttgtc agtctagttg
    21241 accttcgtca tgacccgtca gcagatgatg tgcagatgta cgaatttctc aagtattatg
    21301 agattccagt catcattgtg gcgaccaagg cggacaagat tcctcgtggt aaatggaaca
    21361 agcatgaatc agcaatcaaa aagaaattaa actttgaccc aagtgacgat ttcatcctct
    21421 tttcatctgt cagcaaggca gggatggatg aggcttggga tgcaatctta gaaaaattgt
    21481 gaggaaaaga aaatggcaaa aacaattcat acagataagg ccccaaaggc tatcgggccc
    21541 tatgttcaag gaaaaatcgt tggcaacctt ttgtttgcta gcggtcaagt tcccctatcc
    21601 cctgaaactg gggaaattgt aggagagaat atccaagaac agacagagca agtcttgaaa
    21661 aacatcggtg ctattttggc agaagcagga acagactttg accatgttgt caaaacaact
    21721 tgtttcttga gcgatatgaa cgactttgtt ccttttaatg aggtttacca aacggccttc
    21781 aaagaggaat tcccagctcg ttcagctgtg gaggtagctc gtcttcctcg tgatgtaaaa
    21841 gtcgaaattg aagtcatcgc agagattgga taagctagtt gaagtttggt gttgccaaac
    21901 ttcttttgat ataaggagaa aaagatgaca aagaaacaac ttcacttggt gattgtgaca
    21961 gggatgagtg gcgcagggaa aactgtagcc attcagtcct tcgaggatct aggttatttc
    22021 accattgata atatgccgcc agctctcttg cctaagtttt tgcagctggt tgaaattaag
    22081 gaagacaatc ctaagttggc cttggtagtg gatatgcgta gccgttcttt cttttcagag
    22141 attcaagctg ttttggatga gttggaaaat caagatggtt tggatttcaa aatcctcttt
    22201 ttggatgcgg ctgataagga attggtcgct cgttacaagg aaaccagacg gagtcaccca
    22261 ctagcagcag acggtcgtat tttagatgga atcaagttgg aacgtgaact cttggcacct
    22321 ttgaaaaata tgagccaaaa tgtggtggat acgactgaac tcactccacg tgagctgcgc
    22381 aaaacccttg cagagcagtt ttcagaccaa gaacaagccc agtctttccg tatcgaagtc
    22441 atgtctttcg gatttaagta tggaatcccg attgatgcgg acttggtctt tgatgtccgt
    22501 ttcttgccaa atccctatta tttaccagaa ctgagaaacc aaacgggtgt ggatgaacct
    22561 gtttatgatt atgtcatgaa ccatcctgag tcagaagact tttatcaaca tttattggcc
    22621 ttgattgagc cgattctgcc aagttaccaa aaggaaggta agtccgtttt gaccattgcc
    22681 atgggatgta cgggtggaca acaccgtagt gtggcatttg ctaaatgctt ggcgcaggac
    22741 ttatccaaga attggtctgt taatgaaggg catcgcgaca aagaccgcag aaaggaaacg
    22801 gtaaaccgtt catgagaaaa ccaaagataa cggtgattgg tggagggact ggaattcccg
    22861 tcattctaaa aagtctgcgg gaaaaagatg tggaaatcgc agctatcgtg acggtggcag
    22921 atgatggtgg ttcttcaggt gaactccgaa aaaatatgca acagttgaca ccgccaggtg
    22981 atcttcgtaa tgtccttgtg gccatgtcgg atatgcctaa gttttatgag aaggtctttc
    23041 agtatcggtt ctctgaggat gccggagcct ttgctggcca tccattggga aatctcatca
    23101 ttgctggctt gtcagaaatg cagggttcaa cctataatgc catgcagtta ttgagcaaat
    23161 ttttccatac aacagggaaa atttatcctt ccagtgacca tcctttgacc cttcatgcag
    23221 tctttcagga tgggacagaa gtggctggag agagtcatat tgtagaccat cgaggcataa
    23281 ttgacaatgt ctatgtgacc aatgccctaa acgatgatac gcctctggcc agccgtcgag
    23341 tagtgcagac catccttgaa agtgacatga ttgtcctagg gccaggttcc ctctttacct
    23401 ctattttgcc caatatcgtg attaaggaaa ttgggcgggc tcttttggaa actaaggcag
    23461 aaattgccta tgtctgcaat atcatgaccc aacgtgggga gacggaacac tttacagata
    23521 gcgaccacgt ggaagtcttg catcgtcacc ttggtcgccc ttttatcgac actgtcttgg
    23581 tgaatattga aaaagtgcct caggaataca tgaattccaa ccgttttgat gaatacttag
    23641 tgcaagtgga acacgatttt gtaggtcttt gtaagcaagt ttcgcgcgtg atttcatcta
    23701 acttccttcg tctggaaaat ggcggtgcct tccacgatgg agatttgatt gtggacgagt
    23761 tgatgcgcat tatacaggtg aaaaaatgag tttcacagta gcagtaaaag aagaaatcct
    23821 gggccaacac cacctgagcc ggcatgaatt atctgccatt atcaaaatgt ctggtagcat
    23881 cggtctctcg acttcgggct tgactttgtc agttgtgaca gaaaatgcca aactggcccg
    23941 tcacctctat gagtcctttc tccatttcta tgaaatcaaa tctgaaattc gtcactacca
    24001 acggagcaat cttcgcaaga atcgcgtcta taccgttttt acagatgaaa aggtgcagga
    24061 tttgttaagt gatttgcact tggcagactc tttctttggc ctggaaacag gtattgatga
    24121 ggcgatttta tcggacgagg aagcaggtcg tgcctatctc tgtggcgctt tcttggcaaa
    24181 tgggagcatt cgtgaccctg agtcaggcaa gtaccagttg gaaatcagtt ctgtttatct
    24241 ggaccacgcg caaggaattg cctcccttct ccaacagttt ttactggatg ccaaggtgct
    24301 tgagcgcaag aagggggctg tgacctatct ccagcgagca gaagacatta tggacttctt
    24361 gatagtaatc ggagccatgc aggcacgtga tgattttgag cgggttaaga ttttgcgaga
    24421 aacccgtaac gacctcaatc gggctaataa tgccgagaca gctaatatcg ctcggacagt
    24481 ttctgccagc atgaagacca ttaacaatat cagtaaaatc aaagatatca tgggcttaga
    24541 aaatttgcca gtagatttgc aggaagtggc gcaactgagg attcagcacc cagactactc
    24601 tatccagcag ttagcagata gtctcagcac ccctctgacc aaaagtggtg tcaatcacag
    24661 actcagaaaa atcaataaga tagcggatga attataaaac cacgaatcct atgtgactcg
    24721 tggttctttt ttataaacta gtagagtgtt ttggttgtac tttttgttca gggtcaatgt
    24781 agttgatagc gttgttgaca gcagttggag cttctccgag gcctgtcgca atcagatcaa
    24841 tttttccgtc atagtagcag cagtcaccga tagcatagat acctgcttgg ctggattcct
    24901 gtttgctgtt gacgataatc ttgtgacggt tgaggtcgag cccccagttt ttaaggttac
    24961 cgacagaaga tttgaaacca tagttgacaa agaggtggtc taggtcaatg gtttcagttt
    25021 catcagattt gacttttgtg atttcaagtt tatcaagtgt ttttccattt ccaaggagtt
    25081 ggctaggggc gaatggtgtc ttgatggtta cagatgattc ttgcaaggct tgaacactgt
    25141 gttccaaggc acggaaatta tctctgcggt gaacaagggt agttggtgcg attttttcaa
    25201 aagccaaagc ccaatccaca gccgagtctc ccccaccaag aatcgtcact ttcttaccag
    25261 cgtattgctg aatgttagaa acgtggtagt ggatattttc atagccctca accccttcaa
    25321 gttccagcgg acgtggtttg aaggcaccgc cacccatagc gatgataact gttttagtca
    25381 ggtgacttcc tttagaagtt gtgatggcaa attcttcttg tttgtcaatc tcaagaaccg
    25441 tttcattgag atgaataggg gtatcaaatc catttagctg ttcaatcaag cggttagtca
    25501 actcttctcc agtcaggttt gggaagcctg gtacgtctag gatttccttt tcagggtaga
    25561 gaatagcagg ttgtccacct agctggggaa gagagtcgat gatttgaacc ttggcttggc
    25621 gtaggtgggc ataaaaggct gcaaaaagcc cgacaggacc accacccaca atggtaatat
    25681 catagagttg agacatagtt tctcctttgt tttttctagt cagtttattt tatcatattt
    25741 ttgttcaatg taaaatggac taggataggg aaaaaaatgc cagaaaatgg tataatagga
    25801 cgaaagtgtt ttgataggga ggatatcagg gatgaatttt caacaattat ccaatctgca
    25861 atattggacc agtttgtttg caagtccatg gacgatagct atcaatctga ttgatatttt
    25921 gattgttgct tatattttat accattttac aaaagctatt gcaggaacca agattatgat
    25981 tttggtacgt ggagttttgg tgtttatttt agctcaaatc cttgcaaata tgattggttt
    26041 gactacgatt tcttggttaa tcaatcaaat tattacttat ggggttattg cggcggttgt
    26101 tatcttctct ccagagattc ggactggttt ggaacgtttg ggaagagcga cagatttttt
    26161 ttccaatgcc cctattagtg ctgaggaaca gatgattcgt gcctttgtta agtctgttga
    26221 atacatgagt cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac gtaccttgca
    26281 ggagtatatt tcgacaggaa ttcccttgga tgctaagatt tctgcagaac ttctcattaa
    26341 catttttatt cccaacactc ccctacatga tggtgcggtg attatcaaag aagaacgtat
    26401 cgctgtgacg tctgcctatc tgcccttgac aaaaaacaca gggatttcca aggaatttgg
    26461 gaccagacac cgggcggcta tcggtttatc agaagtctca gatgccttga cttttgtcgt
    26521 atcagaggaa acgggaggaa tttcgataac ctataatgga aggtttaagc acaacctaac
    26581 acttgatgaa tttgaaacag aattacgtga aatcttactt ccaaaagagg aagtgggtct
    26641 tagttttaaa gaacgattgc taggaggatg gaaacatgaa aaaaaatagt ttatatatca
    26701 tatcctcact cttttttgct tgtgtcttat ttgtctatgc tacggcgacg aattttcaaa
    26761 acagtaccag tgctaggcag gtaaaaacgg aaacctatac taatacagta acaaatgtcc
    26821 ctattgacat acgctataat agtgataagt attttattag cggttttgct tcagaagtat
    26881 cagtggtctt gactggggca aatcgcctat cgctagctag tgaaatgcaa gaaagtacac
    26941 gtaaattcaa ggttactgct gacctaacag atgccggtgt tggaacgatt gaagttcctt
    27001 tgagcattga agatttaccc aatgggctga ccgctgtagc gactccgcaa aaaattacag
    27061 tcaagattgg taagaaggct cagaaggata aggtaaagat tgtaccagag attgacccta
    27121 gtcaaattga tagtcgggta caaattgaaa atgtcatggt gtcagataaa gaagtgtcta
    27181 ttacgagtga ccaagagaca ttggatagaa ttgataagat tatcgctgtc ttgccaacta
    27241 gcgaacgcat aacaggtaat tacagtggtt cagtaccttt gcaggcaatc gaccgcaatg
    27301 gtgttgtctt accggcagtt atcactccgt ttgatacaat aatgaaggtg actacaaaac
    27361 cagtagcacc aagttcaagc acatcaaatt caagtacaag cagttcatcg gagacatctt
    27421 cgtcaacgaa agcaactagt tcaaaaacga attaaaaata gaaaaggatt ttataacaat
    27481 gggtaaatat tttgggactg atggagtccg tggagaagct aacctagaac taacaccaga
    27541 attagccttt aaactaggac gttttggagg ctatgttctt agtcaacatg aaacggaagc
    27601 gccgaaagtc tttgtaggac gtgacacacg tatttcaggg gaaatgctgg aatcggcctt
    27661 ggtggcaggt ctcctttcag tagggattca cgtatacaaa cttggtgtcc ttgcaacacc
    27721 agcagtagct tacttggttg aaactgaagg agcaagtgcc ggtgtcatga tttctgctag
    27781 ccacaaccca gcccttgata acggaatcaa gttctttggc ggtgatggct tcaaactaga
    27841 tgatgaaaaa gaagcagaaa ttgaagcctt gctagatgct gaggaagaca ctcttcctcg
    27901 tccaagtgca gaaggcttag gaattttggt agattatcca gaaggcttgc gtaagtatga
    27961 aggatacctt gtttcaactg gaactcctct tgatggaatg aaggttgcct tggatacagc
    28021 taatggagca gcttctacca gtgcccgtca aatctttgca gaccttggtg cccaattgac
    28081 ggttatcggg gaaacaccag acggtcttaa catcaacctt aatgttggtt caacacatcc
    28141 agaagccctt caagaagtgg tcaaagaaag tgggtcagct attggtttgg cctttgatgg
    28201 agacagtgac cgcttgattg ctgttgatga gaatggtgac atcgtcgatg gtgacaagat
    28261 tatgtacatc atcggaaaat acctttctga aaaaggacaa ttggctcaaa atacaattgt
    28321 gacaactgtt atgtctaacc ttggtttcca caaggccttg aatcgcgaag gtattaacaa
    28381 ggcagttact gcagttggtg accgctacgt tgttgaagaa atgagaaaat caggctacaa
    28441 ccttggtggt gaacagtctg gtcacgttat cttgatggat tacaatacca caggtgatgg
    28501 tcaattatca gcagttcaat tgactaaaat catgaaggaa actggtaaga gcttatcaga
    28561 gttggcggca gaagtaacga tttatccaca aaaattagtt aatatccgag tggaaaacgt
    28621 catgaaggaa aaggccatgg aagtgccagc tatcaaggcc atcatcgaga agatggaaga
    28681 agaaatggcg gggaacggcc gtatccttgt tcgtccaagt ggaacagagc ccctcttgcg
    28741 tgttatggca gaagcgccta caacagaaga agtggactac tatgttgata ccatcacaga
    28801 tgtagttcgt gctgaaattg ggattgacta aatcctagaa aactagatga aaataaaaaa
    28861 ttatggtaca atagcttata atattgtagc cgatggagaa agacatgaat cttaaacgtg
    28921 aacaagaatt tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg gaaaatgaaa
    28981 atggagctcc tgaaaccaag gtggatgtga atttccaatt gctccaacat gaccaagaaa
    29041 atcaagtgac ttccttgatt gtcatcttga gctttatgat tgtatttgat aagtttgtca
    29101 ttagtggaac aatctcacaa gtaaaccata tcgatggccg tattgtcaat gaaccaagtg
    29161 aattgaacca agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg ctcaatcgtt
    29221 tgacttacga agtgactgaa attgcattag acttaccagg aattaatttg gagttctaat
    29281 atgaaattag ctgttttcac agattcctct gcttatctca gtgaagagac cttgcaaaga
    29341 gaagacttgt ttgtcttgga tattcctgtc aatattgatg gtgaggaata tgtcgaaggc
    29401 atcaatctgt ctgctgagga attttaccaa aaaatggctc aggcttctga attgcctaag
    29461 accagtcaac caagtattgc caagttagat gagatcttaa cttcgctcaa agaacaaggc
    29521 tatacacatg ccttggggct tttcctatct tctggaattt caggttttta ccaaaatatc
    29581 cagtatatgg tcgatgacta tgagggctta accattgctt tcccagacac tttgattaca
    29641 agtgctcccc taggcatcat ggttgaaagc gtctttaatt ggcgtgatca gggcgatgat
    29701 tttgccagca ttcaggataa gctagccatt caaatcagcc gtacgtcagc ctttatcatg
    29761 gtagatgact tggatcattt ggtaaaaggt ggacgccttt caaatggggc tgccattttg
    29821 ggcaatttgc ttagcattaa gccaatcctt tattttaacg atcaaggtgt gattgaagtt
    29881 tacgaaaaag ttcgtactga aaagaaggcc accaaacgct taattgaaat tatcaaggaa
    29941 acaacggctt caggtcaata ccgggtcatt gtcattcatg ggaatgatcc tgaaaaggct
    30001 gaagaattgc gtcagcattt gcttgatttt ggcttgggtt cggatgtttc acttgctaca
    30061 tttggtagtg tcattggaac gcacctagga gcaggaagta ttgctctggg ttatattcca
    30121 gtgatttagt aggcacttct aggctagtta atcttagcaa ttgaacacgg actacctgcc
    30181 tcttgaaaaa agatgtctgt cttgcctttc gtggaaagtc agtgccattc cctatttttc
    30241 atgggtggct aagatccttt gtattttgat aattgaacac gcctggaacc ctgtgtgaaa
    30301 aagatagttc ttccaaggag tagacacttc ttgatcagaa ttcctatttt cactttgtgt
    30361 tcttacaggc tttgtatctt agacaggagt agagatgagt attcgagtaa ttattgccgg
    30421 ttttaaggga aagatgggtc aagctgcttg ccagatggtc ttgcctgatt cagatttgga
    30481 cttggtagca gttttggatc cttttgagtc tgagtcagaa tggcagggta ttcctgtttt
    30541 caaggataag gctgatttag ctggttttga agcggatgtc tgggtagact ttactactcc
    30601 agctgttgcc tacgaaaata cacgttttgc tcttgaaaat ggctttgctc cagtagttgg
    30661 aacgactggt ttcacgagtg aagaaattgc agagttaaaa gaattttctc gtgcccaaga
    30721 cttgggtggc ctgattgccc ctaactttgc cttgggtgct gtcttactca tgcaatttgc
    30781 gacgcaggct gccaaatatt tcccaaatgt ggagattatt gagctccatc atgacaagaa
    30841 aaaggatgct ccgagtggaa cagccattaa aacagctgag ttgatggcag aggttcgaga
    30901 gtccattcag caaggcgcag cagatgagga agagctgatt gctggtgctc gtggtgctga
    30961 ctttgatggt atgcgcatcc actcagttcg tttgccaggt ttggtagccc atcaggaagt
    31021 catctttggc aatcaaggag aaggattgac cctccgtcat gactcctatg atcgcatctc
    31081 cttcatgaca ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg agcttgtcta
    31141 tggattagaa cacttattat gagattaacg caaatgcctt ctgaatttca gaaggcttta
    31201 ccagtattag aaaaaattaa agaagcaggc tttgaggctt attttgttgg gggctctgtt
    31261 cgagatgccc ttctccatag ccctatccac gatgtggata ttgcgacgtc ttcttatcca
    31321 gaagagacca agcagatttt tccgcgaaca gccgatatcg gaatcgagca tggaaccgtc
    31381 ttggtcttag atggggatga ggagtatgag gtaacaacct ttcggacaga ggatgtctat
    31441 gtggactatc gcagacccag tgcggtttcc tttgtgcgct cgctagaaga agacctcaaa
    31501 cgccgtgatt tcacagtcaa cgcctttgcc ttggatgaga caggagaaat cgttgacttg
    31561 ttccatggtt tagaagattt ggaaaagcaa gtcttgcgag cagttggagt ggctagtgag
    31621 cgtttcaacg aagatgcttt acggattatg cgtggtttcc gttttcaggc tagtcttggt
    31681 tttgcacttg agccagaaac atttaaagct atgaagacct tgacgccgct tttggagaac
    31741 atttctgtag agcgtacctt cgtcgagttt gataaactct tgctggctcc attttggaga
    31801 aggggcttgg cttccatgat tgagagtcaa gcttatgact atctccctga tatggcatct
    31861 agccagaaca agctcaacag actgtttgat ttggagactg attttacttt tgaatcctct
    31921 gaacaagcct gggcggcttt actatgggct ttggagattg aaaatgcgca gtcatttttg
    31981 aaatcttgga agacctcacg ccagtttgcc aagcaagttc aggatttgct gattattttg
    32041 gctctgcgtg aaaatggaga attgagcaag cgagattgtt atcgctttga catagatttg
    32101 cttttacagg ctgaaaatct tcgtcaagct cgaggaaaag aagtcaaccc acaagccatc
    32161 acagaaaaat accaaagctt gactatccat gacaagaaag agattcagat taatggcgat
    32221 attttgatca aggaatatgg ctatcagcca ggcccagact tgggagagat tttaacagag
    32281 attgagtttg ccattgtcga tggagaattg gagaataatc gtgaagccat ccatgcttac
    32341 ctgagggaga aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc gttggtgaca
    32401 agaccgtttt tagggatatt tcctttatta tccatgactt agacagaatt ggtttaatcg
    32461 gtgtcaatgg gactggcaag accacccttt tggacgtcct ttctggtgtt tctggctttg
    32521 atggggatgt cagtcctttt tcagctaaaa atgattacca gattggttac ttgactcagg
    32581 atcctgattt tgatgataga aagacagttt tggatacggt tctatctagt gaactcaagg
    32641 aaatccagct cattcgtgag tatgaattga ttatgctcga ctatagtgag gacaagcagg
    32701 cgcgtttgga acgtgtcatg gcagagatgg actctctcca agcttgggaa atcgagagtc
    32761 aggtcaagac cgttcttagc aaattgggca ttcaagactt atctactcct gttggggaat
    32821 tgtcaggtgg tctgagaaga cgggtacagt tggcacaagt cttacttggc aaccacgacc
    32881 tcttgctttt ggatgagccg accaaccatc tggatattgc gattattgag tggctgaccc
    32941 tctttttgaa aaattctaag aagaccgtcc tttttatcac tcacgatcgt tatttcttag
    33001 acgctttgtc aacacggatt ttcgagttgg agcgagcagg cttgacagaa tatcagggca
    33061 attaccagga ctatgttcgc cttaaggcag aacaggacga gcgcgatgca actctacttc
    33121 ataaaaaaga acaactctac aaacaagaac tggcctggat gcgcagacaa ccgcaggcac
    33181 gtgcgaccaa gcaacaagct cgtatcaatc gtttccatga tctgaaaaag gaagtttcag
    33241 gcagtagtgc tgagacagac ttgactatga actttgaaac cagtcggatt gggaagaaag
    33301 tcatcgagtt tcaggatgtt tcctttgcct atgaaaataa gcccattttg caagatttta
    33361 atctcttggt acaggtcaaa gatcgtatcg gaatcgttgg ggacaatggt gttggaaaat
    33421 caaccctact taacctgatt gcaggaagtc ttgagccgat agcaggacaa gttgtgattg
    33481 gggaaactgt tcgcatcgcc tatttctctc aacaaattga gggtttggat gaaagcaagc
    33541 gtgtgatcaa ttacctgcag gaagtggcag aggaggtcaa gaccagtggt ggttctacga
    33601 cttccatcgc tgagttgctg gagcaattcc tcttcccacg ttcgacgcat gggaccttga
    33661 ttgagaaatt atctggtgga gagaaaaaac gtctttatct cctcaaactg ctcttggaga
    33721 aaccaaatgt tcttctttta gacgagccaa ccaatgactt ggatattgca actttgacag
    33781 tcttagagaa tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt cacgaccgct
    33841 atttcttgga taaggtagcg accaagattc tcgcttttga ggatggcaag attcgtcctt
    33901 tctttggtca ttacaccgac tatcttgatg aaaaagcttt tgaaacagat atggccaatc
    33961 aagtgcaaaa ggccgaaaag gaaaaagtgg tcaaggttcg agaagacaag aaacgcatga
    34021 cctaccaaga aaagcaggag tgggcaagta ttgaaggtga tattgaaacc ttggaaaaac
    34081 gtatcgctgc tattgaagag gaaatgcagg ctaacggctc tgactttggt aagctggcta
    34141 ctctccaaaa agaattggat gagaaaaatg aagcactcct tgaaaaatac gaacgctatg
    34201 agtatctcag tgaatttgat agttaagact attcgaaaat caaagtgcaa actatgtcgg
    34261 cttcacattg ccgactcaag tacagcctgt gaccggcttc ctaatttgca ctttgatttt
    34321 tattgagtat aaaagaatag aaaaatcccg tctcatgaaa ggatttctct attctttttt
    34381 ggtttcttga tgaaagatat tttgccaggt ttcgtggcga cgccagatac tggtatggat
    34441 gataccatct aactcatagc agatgagttt ggttttttga ctgatggaag taatttgaat
    34501 gtccttgata gcagaattta actctttttt ggtcttataa gcctctttat ccatctgctc
    34561 tccatcttgg cgaatataga caaaatcatc agtcaagagt tcttctagtt ggtttccttg
    34621 gtcaatcaat tgttcacgca taagcaattt tttatagaca ttgtctaaaa tttcatcctc
    34681 agggataggt gctggttcga tatggacatc ggtatcaaag acgccaaaac gctcctccag
    34741 catagactcg acctgatccg cgatttcatg gctttcaaaa acagacaagt caggattcat
    34801 ctctagtgta atatccaggt agatgttgct accgtaggtg cgacctcttt gcgatttgac
    34861 cttgctgatt ttgggaattt ccatgatagc cttttggtag tcctcgagca ggcggtcgtc
    34921 aaagccatct gaaagactaa aggaagactc gatgaagata tcataggcag tcttcaagat
    34981 aaagaaagtg atgatgatag caaccagttt atccacaatc ggataattga aactactagc
    35041 taggatggca atggcggttc caagtgaggt aacagcgtca gaaagattgt ccttagcagc
    35101 tgccttcagc gccttggagt tggatttctt actgaggcga gtattgtaga gatagaccac
    35161 aaacataatc gctgcagaaa tgattcctag agttgcacca agaggatcaa tgaccgtttc
    35221 ttcccgactg agaatctttt gaatggtatc tcttagaata tcgaaaccga catagaacat
    35281 gatgatagaa gtgatcaagc ttgccaaatc ttcaatcttc caatgaccaa aacggtggtc
    35341 acggtctgca ggctggcgcg ccatccgaat cccgattaag agggccacat ttccaatgat
    35401 gtccgatacg ttattaaaac catcggccac caaactggat gaatgaagga gatgaccagc
    35461 tgctaattta gctgcagaca agatcaaata ggtcgaaata ctgataatgg ctccacgctc
    35521 agctaacttg agatttgaga tagattgctt cattcaactt cctttctagt cacatagcat
    35581 tctaccatta tactggtttt caaggaaaaa ttcaaataag gcagttttta ctctttgaaa
    35641 actccccaaa atttggtata atggtactac tcaagggagt agctggcaga aacctgtgat
    35701 agtgtcgtca ttccgaattt tatgctgaaa agtatgcttt ccggccctat cttaaacagc
    35761 gagacttgtt atgattaaca ggtctctttt tgtttgtcgt gaaaagatat gacagaaaga
    35821 gagtctgttt tgcacacaat gtcaaggagg agacacatgt caaaagaaca aaaacgccaa
    35881 gcgttttata ctcagagccc tgaagaggtc ttgcaggctg tggatgcgac cgagcaaggt
    35941 ttgtcatcaa gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga actcgaagaa
    36001 ggcgagaaac gatcaatctt ggtcaaattc atcgagcaat ttaaggattt gatgattatc
    36061 atcctagttg cggcagcaat cttgtcagtc gtgacttctg gtggggaaga tatcgcagat
    36121 gccattatca tcctagctgt ggttatcatc aacgctgcct ttggtgttta ccaagaagga
    36181 aaagctgaag aagctatcga agccctcaaa tccatgtcta gtccagttgc ccgcgttctt
    36241 cgtgatggac acatggcaga gattgactct aaagaattgg taccaggcga tatcgttgcc
    36301 cttgaagcag gtgacgtggt accagcggac ctacgtttgc tagaagccaa ctctcttaaa
    36361 attgaagaag cagccttgac gggtgaatct gtaccagtcg aaaaagactt gtcagtcgag
    36421 cttgcgacag atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa ctcaaacgta
    36481 acctatggtc gtgggatggg tgttgttgtc aatacaggta tgtacactga agttggtcat
    36541 attgctggta tgcttcaaga tgcggatgag actgatacac cactcaaaca aaacttgaac
    36601 aacctttcta aggtcttgac ctatgctatc ttggtcattg cccttattac ttttgtagtg
    36661 ggtgtcttca ttcaagggaa aaatccactt ggtgagttgt tgacttctgt tgcccttgcc
    36721 gttgcagcca ttccagaagg acttcctgct atcgttacca tcgttctttc tcttggtact
    36781 caagttttgg ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga aacacttggt
    36841 tcaactgaaa tcatcgcttc tgataagact ggtacgctga ctatgaacaa gatgacagtc
    36901 gaaaaagtct tctacgatgc ggttctacat gactcagctg atgatattga actaggtctt
    36961 gaaatgccac tacttcgttc agttgtcttg gccaatgata cgaaaatcga tgtggaaggt
    37021 aacttgattg gtgacccaac cgaaacagcc tttatccaat atgccttgga caagggctat
    37081 gatgtcaaag gtttcttaga gaaatatcct cgtgtagctg aattgccatt tgactctgac
    37141 cgtaagctca tgtcaacagt tcacccattg ccagatggtc gtttccttgt agcagtcaag
    37201 ggtgcgccag accaactctt aaaacgttgt cttcttcgtg ataaggctgg ggatattgct
    37261 ccgattgatg agaaggttac aaatctcatt catacaaaca attctgaaat ggctcatcaa
    37321 gccttgcgtg tccttgcagg tgcttataag attatcgata gtattccaga aaatctcact
    37381 tctgaagagc ttgaaaatga tttaattttt actggtttga ttgggatgat tgaccctgaa
    37441 cgtcctgaag ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg tccgattatg
    37501 attacaggtg accaccaaga cacagcggaa gccattgcca aacgtttggg aatcattgac
    37561 gcaaacgata cagaaggtca cgttttaact ggtgctgaac tcaatgaact gtcagatgaa
    37621 gaatttgaaa aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc agaacacaag
    37681 gttcgtatcg tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac aggtgacggt
    37741 gtcaatgacg cgccagctct gaaaacagcc gatatcggta tcggtatggg aatcactggt
    37801 acagaggttt ctaagggggc ttctgatatg attcttgcag atgataactt tgcgactatt
    37861 atcgtcgcag tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac tattcagtac
    37921 ctactttctg ctaatactgc tgaagtatta accatcttcc tatcaacctt gtttggttgg
    37981 gatgtcttac agccggttca tcttttgtgg atcaacttgg taacggatac cttcccagct
    38041 atcgctcttg gtgttgaacc tgcggaacct ggtgtcatga atcataaacc acgtggacgc
    38101 aaggcaagct tcttctcagg tggtgttttg agttctatca tttatcaagg tgtactccaa
    38161 gcagctcttg ttatgagtgt ttatggcctt gcgattgctt acccagttca tgtgggtgac
    38221 aatcatgcta ttcatgcaga tgccctaacg atggcctttg caacccttgg tttgattcag
    38281 ctcttccatg cctacaatgt caagtctgtc taccaatcca tcttgacagt tggcccattc
    38341 aagtctaaga cctttaactg gtccatcttg gtatccttta tccttctcat ggcaacaatc
    38401 gttgtagaac cgcttgaagg aatcttccac gtaaccaaac tagacttgtc acaatgggga
    38461 attgttatgg ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa gtttattcaa
    38521 cgcaaactcg gttttgacaa gaatgcgatt taagggaggt ttcggatgtc agcatatcaa
    38581 ttaccgaccg tatggcagga tgaagctagt aatcaaggag cttttacggg gctaaacaga
    38641 ccaacagcag gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc ttttcagctt
    38701 tattcactgg gaacaccaaa tggtgtgaag gttactatct tattggaaga attactagaa
    38761 gctggtttta aggaagcggc ttacgacttg tataagattg ctatcatgga tggggatcaa
    38821 ttcggctcag actttgtgaa gctcaatcca aattccaaga ttccagcctt attggaccag
    38881 tcaggtactg aaaacgtaag agtctttgag tctgctcata ttcttcttta ccttgctgag
    38941 aaatttggag cctttttacc aagtaatcct gtggaaaagg tagaagtttt gaattggcta
    39001 ttctggcaag caggtgcagc accttttcta ggtgggggat ttggacattt cttcaattat
    39061 gctcctgaaa aattggaata tcctattaac cgttttacga tggaagtgaa acgccagttg
    39121 gatttattgg ataaggaatt ggctcagaaa ccttatattg caggcaatga ctatacgatt
    39181 gcagatattg ctatctggtc ttggtatgga cagttagttc aaggaaatct ttaccaaggt
    39241 tctgcaaaat tcttggatgc ctcaagttat caaaatctag taaaatgggc agaaaaaatt
    39301 gccaatcgtc cagctgttaa gcgtggcttg gaagtaactt atacagaaat taaatagcaa
    39361 aaaagtcatc ttcggatggc tttttgctac agttgaggga aagggtttgc agttatcggc
    39421 ttttgtgcta taattgctat aatatagtaa aaagcaagag gagtgtgagg aagtggaaaa
    39481 gaaaattgtg aaggatatct tatttttatc tcaagtgtct cagccggcaa gtcaggagga
    39541 cctttatctt gccagagatt tgcaggatac actcttagca aatcgtgata cctgtgttgg
    39601 tctagctgcc aatatgattg gggtgcagaa gcgcgtgatt atctttaatc ttggcttggt
    39661 tcccgtggtc atgtttaacc cagtgcttct gtcctttgaa ggatcctatg aggcagaaga
    39721 aggctgtttg tccttggtag gtgtgagatc aactaagcgt tatgaaacca caaggcttgc
    39781 ctatcgtgac agcaagtggc aggaacagac cattaccttg acaggcttcc cagctcagat
    39841 ttgccagcat gagctggatc acttggaagg acgaatcatt taggaggaaa gcaaatgaaa
    39901 cgaatagtct ttgaacttat ttttatcgca acgacctggt atatcttttt accgcccctt
    39961 aacctgacca gctgggaatt tctcttcttc ctctgtgggc atttgttagt tgtggcaatc
    40021 ttatttggct ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg ccacggtaag
    40081 gcggaagctg ccttaaatct tgagggtttc aaaatcaatc ggttagggaa aattctgtta
    40141 gcttcgattg gaggaattct tctcttggca gctttggttt ccttggtaac ttccagcatg
    40201 tttcaggcta aaaattatgc caatgtagtc acggttacgg aaaaagactt tactgaattt
    40261 cctaggagtg acaccagtaa ggttcctatc ctagatagaa gtactgctga aaaaattgga
    40321 gaccgctact tgggttccct aaccgataag gtgtcgcaat acgtagcggc agatacctat
    40381 acccaattga caattgatgg gaaaccttat cgggtcacac cactagaata tgcagaccct
    40441 atcaaatggt ttaacaatca agccaaggga atcggtgagt atatcaaggt ggacatggta
    40501 actggaaatg cggatttggt ggacttgaag acaccaatca agtattcaga ctcggagtat
    40561 tttaaccgtg atgtcaaacg tcacctgcgc ttgaagtacc cgactaaaat ctttaaaact
    40621 ccatcttttg aggtggacga tgagggcaat cctttctatg tagcaacggt ttaccaaaag
    40681 caatttggac ttgcggttcc tcgtcctgct tcagtcatta tcttggatgc tacaaatgga
    40741 gaaaccaagg aatacagctt atcagatgtt ccagaatggg tggacaggat ctatccagca
    40801 gaggaaacca ttgagcaaat caactacaac ggcaagtaca aggacggttt cttgaatgcc
    40861 atgatttcca agaaaaacgt gacccagact accaatggct ataattactt gtctatcggt
    40921 aatgacatct atctctacac aggtgtgacg tcggctaatg cggatgagag taatcttggt
    40981 ttcatccttg aaaatatgcg aacaggagaa attactaagt atagcttggc ttctgcgaca
    41041 gaagaatcag cccgtgaatc agcagaaggt gctgttcagg agaaatccta caaagcaacc
    41101 ttcccaatcc tcatcaacct caatgacaag cctctctaca tcatgggctt aaaggacaat
    41161 gctggcttgg tcaaagagta cgccctggta gacgcagtcg agtaccaaaa tgttatcgtt
    41221 gctactacag tggaagagat gctcagcaag tatgccaata aaaacgacct tgaaattgac
    41281 aatgcaacga cagaaagcat caagggagta gtagcagacc tcaaatcagc tgttatcaag
    41341 ggagacactg tctacttctt taaagttgat ggcaagatat acaaggtaaa ggcttcagta
    41401 tccgatgacc ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt aggaaaagac
    41461 aattatctca agacctttaa agtacaataa tactcttcga aaatctctag ctctatctgc
    41521 aacctcaaaa cagtg
//
LOCUS       DAVMMR010000012        40954 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMMR010000012 DAVMMR010000000
VERSION     DAVMMR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.012"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            164..2146
                     /locus_tag="VYS74_000618"
     CDS             164..2146
                     /locus_tag="VYS74_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3788694.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2447..7750
                     /locus_tag="VYS74_000619"
     CDS             2447..7750
                     /locus_tag="VYS74_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3788695.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTASTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMEALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQSSMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKMPDYKQEIA
                     RPETPKQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7917..10076)
                     /locus_tag="VYS74_000620"
     CDS             complement(7917..10076)
                     /locus_tag="VYS74_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEV3788696.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10073..10669)
                     /gene="recU"
                     /locus_tag="VYS74_000621"
     CDS             complement(10073..10669)
                     /gene="recU"
                     /locus_tag="VYS74_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV3788697.1"
                     /translation="MVNYPHKVSSQKRQKSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10735..11262
                     /locus_tag="VYS74_000622"
     CDS             10735..11262
                     /locus_tag="VYS74_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV3788698.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11332..11661
                     /gene="gpsB"
                     /locus_tag="VYS74_000623"
     CDS             11332..11661
                     /gene="gpsB"
                     /locus_tag="VYS74_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV3788699.1"
                     /translation="MASIIFSAKDIFDQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11682..12062
                     /gene="rnpB"
                     /locus_tag="VYS74_000624"
     ncRNA           11682..12062
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYS74_000624"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12147..13304
                     /locus_tag="VYS74_000625"
     CDS             12147..13304
                     /locus_tag="VYS74_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV3788700.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13317..14711
                     /gene="mapZ"
                     /locus_tag="VYS74_000626"
     CDS             13317..14711
                     /gene="mapZ"
                     /locus_tag="VYS74_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV3788701.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNMTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14787..16211
                     /gene="gndA"
                     /locus_tag="VYS74_000627"
     CDS             14787..16211
                     /gene="gndA"
                     /locus_tag="VYS74_000627"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV3788702.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16223..16912
                     /locus_tag="VYS74_000628"
     CDS             16223..16912
                     /locus_tag="VYS74_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788703.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17010..17975
                     /gene="cbpC"
                     /locus_tag="VYS74_000629"
     CDS             17010..17975
                     /gene="cbpC"
                     /locus_tag="VYS74_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV3788704.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            17994..18992
                     /gene="cbpJ"
                     /locus_tag="VYS74_000630"
     CDS             17994..18992
                     /gene="cbpJ"
                     /locus_tag="VYS74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV3788705.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(19110..20342)
                     /locus_tag="VYS74_000631"
     CDS             complement(19110..20342)
                     /locus_tag="VYS74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3788706.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20353..20973)
                     /locus_tag="VYS74_000632"
     CDS             complement(20353..20973)
                     /locus_tag="VYS74_000632"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788707.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKVDLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(20873..21211)
                     /locus_tag="VYS74_000633"
     CDS             complement(20873..21211)
                     /locus_tag="VYS74_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEV3788708.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
     gene            21874..22203
                     /locus_tag="VYS74_000634"
     CDS             21874..22203
                     /locus_tag="VYS74_000634"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788709.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TSCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            22325..23203
                     /gene="mvk"
                     /locus_tag="VYS74_000635"
     CDS             22325..23203
                     /gene="mvk"
                     /locus_tag="VYS74_000635"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV3788710.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            23185..24138
                     /gene="mvaD"
                     /locus_tag="VYS74_000636"
     CDS             23185..24138
                     /gene="mvaD"
                     /locus_tag="VYS74_000636"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV3788711.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            24125..25132
                     /locus_tag="VYS74_000637"
     CDS             24125..25132
                     /locus_tag="VYS74_000637"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV3788712.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRSFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            25116..26126
                     /locus_tag="VYS74_000638"
     CDS             25116..26126
                     /locus_tag="VYS74_000638"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV3788713.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            26203..26901
                     /locus_tag="VYS74_000639"
     CDS             26203..26901
                     /locus_tag="VYS74_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV3788714.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            26898..27893
                     /locus_tag="VYS74_000640"
     CDS             26898..27893
                     /locus_tag="VYS74_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3788715.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            27907..28539
                     /locus_tag="VYS74_000641"
     CDS             27907..28539
                     /locus_tag="VYS74_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788716.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            28540..28782
                     /locus_tag="VYS74_000642"
                     /pseudo
     CDS             28540..28782
                     /locus_tag="VYS74_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            28707..29018
                     /locus_tag="VYS74_000643"
     CDS             28707..29018
                     /locus_tag="VYS74_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002876249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV3788717.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKTMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            29043..29309
                     /locus_tag="VYS74_000644"
                     /pseudo
     CDS             29043..29309
                     /locus_tag="VYS74_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            29459..29698
                     /locus_tag="VYS74_000645"
     CDS             29459..29698
                     /locus_tag="VYS74_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788718.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            29743..30372
                     /locus_tag="VYS74_000646"
     CDS             29743..30372
                     /locus_tag="VYS74_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3788719.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVCYLKEFRNLNSKDEREYDLALLILEEPIGAKLG
                     TLGFPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            30671..31030
                     /locus_tag="VYS74_000647"
     CDS             30671..31030
                     /locus_tag="VYS74_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3788720.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            31224..32507
                     /locus_tag="VYS74_000648"
     CDS             31224..32507
                     /locus_tag="VYS74_000648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV3788721.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(32555..34921)
                     /locus_tag="VYS74_000649"
     CDS             complement(32555..34921)
                     /locus_tag="VYS74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV3788722.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(35049..35663)
                     /gene="lepB"
                     /locus_tag="VYS74_000650"
     CDS             complement(35049..35663)
                     /gene="lepB"
                     /locus_tag="VYS74_000650"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV3788723.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            complement(35667..36548)
                     /locus_tag="VYS74_000651"
     CDS             complement(35667..36548)
                     /locus_tag="VYS74_000651"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV3788724.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVITRDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            36635..36937
                     /gene="zapA"
                     /locus_tag="VYS74_000652"
     CDS             36635..36937
                     /gene="zapA"
                     /locus_tag="VYS74_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV3788725.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            36934..37482
                     /locus_tag="VYS74_000653"
     CDS             36934..37482
                     /locus_tag="VYS74_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV3788726.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            37586..39922
                     /locus_tag="VYS74_000654"
     CDS             37586..39922
                     /locus_tag="VYS74_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV3788727.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLGPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGAALNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESRVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQVDQD
                     NDVNRIIEQLEEQTLESHKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKILQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQVQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(39972..40901)
                     /locus_tag="VYS74_000655"
     CDS             complement(39972..40901)
                     /locus_tag="VYS74_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001150386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3788728.1"
                     /translation="MQDESIYGLVYKTIREERGFTQKEAAGTTVTPHFLRQFEQGKSR
                     ITIQKFEEILSNIGIDKETFDSLKTQMFPTEFHKLQAQVADLAYKREFKKIISLINQP
                     MENPGIAEHYVIANRVVNKFAIANIIGDSFLTPKDYQELEYIKNYLSELDDWNKIEVN
                     IFSSILPHFSIEFLDYRLYHLLDTLKKQTEYHSIRTADYYIACLRTAIKHYSVNGYYD
                     KAESLAVKTLEVINTFPLLSTKMTEMISLSMERANNFLRKDDVRGLELAKHIFASLDN
                     FEKVYPNQLLTRMREDFFVTVTQLNHTGQPLDV"
BASE COUNT        12482 a         7503 c         9213 g        11756 t
ORIGIN      
        1 aaaaattcct tgactatgtg atatagttga gggattttta aatgatattc atattttttg
       61 caaagatgtt gtttgaaaaa taattttcaa aaattctgaa aattctgttg acaactttct
      121 gaaaagagtc tataatggag agaaagtttt aaaggagaaa atgatgaaaa gttcaaaact
      181 acttgccctt gcgggcgtga cattattggc ggcgactact ttagctgcat gctctggatc
      241 aggttcaagc gctaaaggtg agaagacatt ctcatacatt tatgagacag accctgataa
      301 cctcaactat ttgacaactg ctaaggctgc gacagcaaat attaccagta acgtggttga
      361 tggtttgcta gaaaatgatc gctacgggaa ctttgtgccg tctatggctg aggattggtc
      421 tgtatccaag gatggattga cttacactta tactatccgt aaggatgcaa aatggtatac
      481 ttctgaaggt gaagaatacg cggcagtcaa agctcaagac tttgtaacag gactaaaata
      541 tgctgctgat aaaaaatcag atgctcttta ccttgttcaa gaatcaatca aagggttgga
      601 tgcctatgta aaaggggaaa tcaaagattt ctcacaagta ggaattaagg ctctggatga
      661 acagacagtt cagtacactt tgaacaaacc agaaagcttc tggaattcta agacaaccat
      721 gggtgtgctt gcgccagtta atgaagagtt tttgaattca aaaggagatg attttgccaa
      781 agctacggat ccaagtagtc tcttgtataa cggtccttat ttgttgaaat ccattgtgac
      841 caaatcctct gttgaatttg cgaaaaatcc gaactactgg gataaggaca atgtgcatat
      901 tgacaaagtt aaattgtcat tctgggatgg tcaagatacc agcaaacctg cagaaaactt
      961 taaagatggt agccttacag cagctcgtct ctatccaaca agtgcaagtt tcgcagagct
     1021 tgagaagagt atgaaggaca atattgtcta tactcaacaa gactctatta cgtatctagt
     1081 tggtacaaat attgaccgtc agtcctataa atacacatct aagaccagcg acgaacaaaa
     1141 ggcatcgact aaaaaggctc tcttaaacaa ggatttccgt caggctattg catttggatt
     1201 tgaccgtaca gcctatgcct ctcagttgaa tggacaaact ggagcaagta aaatcttgcg
     1261 taatctcttt gtgccaccaa catttgttca agcagatggt aaaaactttg gcgatatggt
     1321 caaagagaaa ttggtcactt atggggatga atggaaggat gttaatcttg cagattctca
     1381 ggatggtctt tacaatccag aaaaagccaa ggctgaattt gctaaagcta aatcagcctt
     1441 acaagcagaa ggtgtgacat tcccaattca tttggatatg ccagttgacc aaacagcaac
     1501 tacaaaagtt cagcgcgtcc aatctatgaa acaatccttg gaagcaactt taggagctga
     1561 taatgtcatt attgatattc aacaactaca aaaagacgaa gtaaacaata ttacatattt
     1621 tgctgaaaat gctgctggcg aagactggga tttatcagat aatgtcggtt ggggtccaga
     1681 ctttgccgat ccatcaacct accttgatat tatcaaacct tctgtaggag aaagtactaa
     1741 aacatattta gggtttgact caggggaaga taatgtagct gctaaaaaag taggtctata
     1801 tgactacgaa aaattggtta ctgaggctgg tgatgaggct acagatgttg ctaaacgcta
     1861 tgataaatac gctgcagccc aagcttggtt gacagatagt gctttgatta ttccaactac
     1921 atctcgtaca gggcgtccaa tcttgtctaa gatggtacca tttacaatac catttgcatt
     1981 gtcaggaaat aaaggtacaa gtgaaccagt cttatataaa tacttggaac ttcaagacaa
     2041 ggcagtcact gtagatgaat accaaaaagc tcaggaaaaa tggatgaaag aaaaagaaga
     2101 gtctaataaa aaggctcaag aagatctcgc aaaacatgtg aaataactgt tgcaaaatat
     2161 aagaaaggat ttagtatttc tcttgaatgc tgaatccttt tttacatttg taaagaaaga
     2221 ttctaaatgt attgaccccc aaaagttgga gcctcttttt gtcagaatag agaaaatttt
     2281 tgttaatttt acttgtttcc tattgctttc tcagctatta tttgttatat taaaagtata
     2341 attatttttt atttatcagg gttaagcatt gcactttcag aggaaggagt attttttaaa
     2401 aaggaaatgt aaacgcttgc tcaaaaatga aaggatttag aagtttatga ataaaggatt
     2461 atttgaaaaa cgttgtaaat atagtattcg gaaattttca ttaggtgttg cttctgttat
     2521 gattggagct gcattctttg ggacaagtcc ggttcttgca gatagcgtgc agtctggttc
     2581 cacggcgaac ttaccagctg atttagctac tgctcttgca acagcaaaag agaatgatgg
     2641 gcgtgatttt gaagcgccta aggtgggaga agaccaaggt tctccagaag ttacagatgg
     2701 acctaagaca gaagaagaac tattagcact tgaaaaagaa aaaccggctg aagaaaaacc
     2761 aaaagaggat aaacctgcag ctgctaaacc tgaaacacct aagacggtaa cccctgaatg
     2821 gcaaacggta gagaaaaaag aacaacaggg aacagtcact atccgagaag aaaaaggtgt
     2881 ccgctacaac caattatcct caactgctca aaatgataac gcaggtaaac cagccctgtt
     2941 tgaaaagaag ggcttgaccg ttgatgccaa tggaaatgca actgttgatt taaccttcaa
     3001 agatgattct gaaaagggca aatcacgctt tggtgtcttc ttgaaattta aagataccaa
     3061 taataatgtt tttgtcggtt atgacaagga tggctggttc tgggagtata aatctccaac
     3121 agctagcact tggtatagag gtagtcgtgt tgctgctcct gaaacaggat caacaaaccg
     3181 tctctctatc actctcaagt cagacggtca gctaaatgcc agcaataacg atgtcaatct
     3241 ctttgacaca gtgactctac cagctgcggt caatgaccat cttaaaaatg agaagaagat
     3301 tcttctcaag gcgggctctt atgacgatga gcgaacagtt gttagcgtta aaacggataa
     3361 ccaagagggg gtaaaaacag aggatacccc tgctgaaaaa gaaacaggtc ctgaagttga
     3421 tgatagcaag gtgacttatg acacgattca gtctaaggtt ctcaaagcag tgattgacca
     3481 agccttccct cgtgtcaagg aatacagctt gaatggacat actttgccag gacaggttca
     3541 acagttcaac caagtcttta tcaataacca ccgaatcacc cctgaagtca cttataagaa
     3601 aatcaatgag acaacagcag agtacttgat gaagcttcgc gatgatgctc acttaatcaa
     3661 tgcggaaatg acagtacgct tgcaagttgt ggacaatcaa ttgcactttg atgtgaccaa
     3721 gattgtcaac cacaatcaag tcactccagg tcaaaagatt gatgacgaaa gaaaactact
     3781 ttcttctatt agtttcctcg gcaatgcttt agtctctgtt tctagtgatc aaactggtgc
     3841 taagtttgat ggggcaacca tgtcaaacaa tacgcatgtc agcggagatg atcatatcga
     3901 tgtaaccaat ccaatgaaag acctagccaa gggttacatg tatggatttg tttctacaga
     3961 taagcttgct gctggtgttt ggagtaactc tcaaaacagc tatggtggtg gttcgaatga
     4021 ctggactcgt ttgacagcct ataaagaaac agtcggaaat gccaactatg taggaatcca
     4081 cagctctgaa tggcaatggg aaaaagctta taagggcatt gttttcccag aatacacgaa
     4141 ggaacttcca agtgctaagg ttgttatcac tgaagatgcc aatgcagaca agaaagtcga
     4201 ttggcaggat ggtgccattg cttatcgtag cattatgaac aatcctcaag gttgggaaaa
     4261 agttaaggat atcacagctt accgtatcgc gatgaacttt ggttctcaag cacaaaaccc
     4321 attccttatg accttggatg gtatcaagaa aatcaatctc cacacagatg gtcttgggca
     4381 aggtgttctc cttaaaggat atggtagcga aggccatgac tctggtcact tgaactatgc
     4441 tgatattggt aagcgtatcg gtggtgtcga agacttcaag accctaattg agaaggctaa
     4501 gaaatatgga gctcatctag gtatccacgt taacgcttca gaaacttatc ctgagtctaa
     4561 atacttcaat gaaaaaattc tccgtaagaa tccagatgga agctatagct atggttggaa
     4621 ctggctagat caaggtatca acattgatgc tgcctatgac ctagctcatg gtcgtttggc
     4681 acgttgggaa gatttgaaga aaaaacttgg tgacggtctc gactttatct atgtggacgt
     4741 ttggggtaat ggtcaatcag gtgataacgg tgcctgggct acccacgttc ttgctaaaga
     4801 aattaacaaa caaggctggc gctttgcgat cgagtggggc catggtggtg agtacgactc
     4861 taccttccat cactgggcag ctgacttgac ctacggtggc tacaccaata aaggtatcaa
     4921 cagtgccatc acccgcttta tacgtaacca ccaaaaagat gcttgggtag gggactacag
     4981 aagttatggt ggtgcagcca actatccact gctaggtggc tacagcatga aagactttga
     5041 aggctggcaa ggaagaagtg actacaatgg ctatgtaact aacttatttg cccatgacgt
     5101 catgaccaag tacttccaac acttcactgt aagtaaatgg gaaaatggta caccggtgac
     5161 tatgaccgat aacggtagca cctataaatg gactccagaa atgcgagtgg aattggtaga
     5221 tgctgacaat aataaagtag ttgtaactcg taagtcaaat gatgtcaata gtccacaata
     5281 tcgcgaacgt acagtaactc tcaacggacg tgtcatccaa gatggttcag cttacttgac
     5341 tccttggaac tgggatgcaa atggtaagaa actttctact gataaggaaa agatgtacta
     5401 cttcaatacg caggccggtg caacaacttg gacccttcca agcgattggg caaagagcaa
     5461 ggtttacctt tacaagctaa ctgaccaagg taagacagaa gagcaagaac taactgtaaa
     5521 agatggtaaa attaccctag atcttctagc aaatcaacca tacgttctct atcgttcgaa
     5581 acaaaccaat cctgaaatgt catggagtga aggcatgcac atctatgacc aaggatttaa
     5641 cagtggtacc ttgaaacatt ggaccatttc aggcgatgct tctaaggcag aaattgtcaa
     5701 gtctcaaggg gcaaacgata tgcttcgtat tcaaggaaac aaagaaaaag ttagtctcac
     5761 tcagaaatta actggcttga aaccaaatac caagtatgcc gtttatgtcg gtgtcgataa
     5821 ccgtagtaat gccaaggcga gcatcactgt aaatactggt gaaaaagaag tgactactta
     5881 taccaataag tctctcgccc tcaactatgt aaaagcctat gcccacaata cacgtcgtaa
     5941 caatgctaca gttgacgata caagttactt ccaaaacatg tacgccttct ttacaactgg
     6001 atcggacgta tcaaatgtta ctctgacatt gagtcgtgaa gctggtgatg aagcaactta
     6061 ctttgatgaa attcgtacct ttgaaaacaa ttcaagcatg tacggagaca agcatgatac
     6121 aggtaaaggc accttcaagc aagactttga aaatgttgct cagggtatct tcccatttgt
     6181 agtgggtggt gtcgaaggtg tcgaagacaa ccgcactcac ttgtctgaaa aacacgatcc
     6241 atatacacaa cgtggttgga atggtaagaa agtcgatgat gttatcgaag gaaattggtc
     6301 actcaagaca aatggactag tgagccgtcg taacttggtt taccaaacca tcccacaaaa
     6361 cttccgtttt gaagcaggta agacctaccg tgtaaccttt gaatacgaag caggttcaga
     6421 caatacctat gcttttgtag tcggtaaggg agaattccag tcaggtcgtc gtggtactca
     6481 agcaagcaac ttggaaatgc atgaattgcc aaatacttgg acagattcta agaaagccaa
     6541 gaaggcaacc ttcctcgtga caggtgcaga aacaggggat acttgggtag gtatctactc
     6601 aactggaaat gcaagtaata ctcgtggtga ttctggtgga aatgccaact tccgtggtta
     6661 taacgacttc atgatggata atcttcaaat cgaagaaatt accctaacag gtaagatgtt
     6721 gacagaaaat gctctgaaga actacttgcc aacggttgcc atgactaact acaccaaaga
     6781 gtctatggag gctttgaaag aggcggtctt taacctcagt caggccgatg atgatatcag
     6841 tgtggaagaa gcgcgtgcag agattgccaa gattgaagcc ttgaagaatg ctttggttca
     6901 gaagaaaacg gctttggtag cagatgactt tgcaagtctt acagctcctg ctcaggctca
     6961 agaaggtctt gcaaatgcct ttgatggaaa cttatctagt ttatggcata catcatgggg
     7021 cggaggagat gtaggcaagc ctgcaaccat ggtcttgaaa gaaccaaccg aaataactgg
     7081 acttcgttat gttccacgtg gatcaggttc aaatggtaac ttgcgtgatg tgaaacttgt
     7141 tgtgacagat gagtctggca aggagcatac ctttactgca actgattggc cagataacaa
     7201 taagccaaaa gacattgact ttggtaagac aattaaggct aagaaaattg tccttacagg
     7261 tactaagact tacggagatg gtggcgataa ataccaatct gcagcggaac ttatctttac
     7321 tcgtccacag gtagcagaaa cacctcttga cttgtcaggc tatgaagcag ctttggctaa
     7381 ggctcagaaa ttaacagaca aagacaatca agaggaagta gctagcgttc agtcaagcat
     7441 gaaatatgcg acggataacc atctcttgac ggaaagaatg gtggaatact ttgcagatta
     7501 tctcaaccaa ttaaaagatt ctgctacgaa accagatgct ccaactgtag agaaacctga
     7561 gtttaaactt agctctttag cttccgatca aggtaagatg ccagattata agcaagaaat
     7621 agctagacca gaaacaccta aacaaatctt gccagcaaca ggtgagagtc aatctgacac
     7681 agccctcttc ctagcaggtg ttagcctagc cctatctgct ctctttgtag taaaaacgaa
     7741 gaaagactag tatttagtaa aacctcttaa caagattacg gaagcagtct ctatcttttc
     7801 caatgaggtt tatagtacag aaaaagcctg agaagatgtc ttctcaggct tttgttaagc
     7861 acataaatac aatagtgcta tgaaaaaatc acccagaaaa atctgggtga taaatgttat
     7921 ggttgtgctg gttgaggatt ctgattttgt tgatcagggg ttgtatttga ttgttgcgta
     7981 ttattgttag gattggtagt cgtactatta tttgtgcttg gagtggttga gctagactgt
     8041 gaagttgaac tatctgatga tgagcttgaa ctttcagttg atgggggttg ttgtggagca
     8101 ggtgagttcc acgtagaacg agcaccattt ttaaatacga attctccatt tctgtagagc
     8161 ccctctggta tattccaatc ttctggattg cttccttcag acaggtaggt catcatagag
     8221 cggtaaactt tggcagcgac cgtaaggcca ttgcctacaa gtggtgtcag acggttagaa
     8281 tagcctgtcc atacagccat tgaatattta cgcgtatagc cagcaaatag ttcatcaggt
     8341 gctacaaatt gagaggtctt gatgtggttt tcaatttcct cgtctgtata gttagaggtt
     8401 cctgttttac cagcctgagg aagccaagca agataggcat ttcgtccagt tccataagtc
     8461 aagactgttt tcatcatgtc ggtcatcata taggctgtcg tttctttcat ggcacgagtt
     8521 ccgacattag agaactcttt ttcactccca tcactaaaga cgactttatg gatatacatt
     8581 ggtttatagt aagttccacc atttgcaaag gcagcgtaag cagcagccat cttttcacta
     8641 cttgctccat attttttgtc tgattcggtt gtgttacttg aaatggcatt tgagtagtga
     8701 atacttgggt agtcgattcc gagaccattt aggaaagtct tggcgcggtt gagtccgacc
     8761 ttgtttagag tttccacggc tgggacgttt cgcgattgtt gcagggcgta ttgcaaggtg
     8821 atgttgccaa agtagcccct atcccagtta taaacaggag tatttgtccc agggtaatta
     8881 tagggctcat cgtgaacgat agtagcagtt gaatcgtaga caccgtactc caaggcagga
     8941 gcatagtctg tgatcggttt catagttgat ccccagtcgc ggtttgtttc tactgcttgg
     9001 ttaattccga aggaaacatt acttgactga tggcgtgctc ctagctgggc aatgacttta
     9061 ccgttagaaa catcaacaat ggtagaagcg acttgcaatt catcgtctgg ataggcaacg
     9121 tattcgtctg tattgtaaat atcccacaga tgtttttgag cttcttggtc tacatttgtg
     9181 tagacatcca tcccagttgt gagtaggtta tagcctgttt cttcttcaac ttgattgatg
     9241 acttccttga ggtaattatc catgtaagca gggtaattac ttgctgattt gagactttgt
     9301 agcccatcag taattggtgt attgactgct ttctcatact gttcagcaga gatgtagcct
     9361 tgatttttca tttcagataa gaccaagttt cggcggtctt gggctgcttc tggatgtgaa
     9421 taggggtcat attggtttgg tgcctgaggc attccagcca gcaaggctaa ctgaggtaaa
     9481 cttaaattat tgaggtcttt accatagtag ttttgagctg ctgtctgcat tccatagttc
     9541 ccattagaca tgtagacctt atttatatag taggtcaaga tttcttgctt ggttgctttt
     9601 tgttctaact gaatcgctaa ccaagcttcc tgagccttac gagaaatagt ctggtcggaa
     9661 gtcgaagttg aaaagtaagt caacttaatc aactgttgag tgagagttga tccaccttgg
     9721 agggaattgc tttgcagatt gcgcaagaaa gctcccagga tacggatggt atcaatcccc
     9781 ctgtggtcga agaagcgatg gtcttcgata gaaacgattg ccttaaccaa atctgtggga
     9841 atatcattag cttgggcatt gacgcggcgt tcagaaccca agtcagcaat gagttgattt
     9901 ttattgtcgt agattttact agaagttgtt gcaactagtt tactctcgga taggctagga
     9961 gccttgctaa cgtagtagaa aaaaactcct ccgcctaaga caatggctgc gataaccaag
    10021 cttaagaagc taatgctcag atacttgatt aggcgcagaa tcgttggttt gttcatcttg
    10081 ttttaccacc taataaatgt tctttgataa cattgagata aggaatttga gggaaggcac
    10141 cagccttgat ttcatatcca tattctcgaa tatattcaag tggcattgat ttttgtccct
    10201 tatcttgatg atagaagcga atcaaatcga atgccggcaa taagtaggtt tcttgctgag
    10261 aagaaaagtg aagaaggaca aagcagattc cttgttgggc aaggacttgt tccatatgct
    10321 gaatctgatg tggatgaaaa tttttcatcg gaatcgcacg tttttgtttt gtttccttga
    10381 cttcaaagtc gatgtaatat ccattataaa cgccagaata gtccgtcgtt gaagcttgtc
    10441 gaaaataggc ttcaacaatc ttggcacgac ttcgttgtgg atagtccact tgtacgattt
    10501 gaataggagt tggtttctta tgtatgacag ccaagccctg agacaaatag tagtcgttgg
    10561 tagcattgat catcttttca aaagacattc ctcgatttgc gaaatttttg ggttgagaaa
    10621 gagatttttg tcttttttgt gatgaaactt tatgtggata gttgaccata attctcctta
    10681 ttggtacaat aacatcactt tattatacca taatttgcac agaaagggtt aaaaatggct
    10741 acagctttgg ttttaggcta ttctgctttt gatttgggtt tattttctga taaggatcct
    10801 cgtcttaaat tgattaaaaa agcgatacgt aaagatttag aggctatggc agcagatggg
    10861 gtgtcttggc ttgtgtttac aggaagtttg ggttttgaat attgggtttt agaggttgct
    10921 caggagatga aaaccgaata cggtttccag ttggccacca tttttgcttt tgaaacccat
    10981 ggggaaaatt ggaatgaagg caatcagatg aaactgagtc gctttaagca ggtagacttt
    11041 gtcaaatatg cttatcctcg ctatgaacac aaggggcagt taagagatta ccagcagttt
    11101 ttgctggaaa acacgactag ttcctatctt ttttatgatg aagaaaatga aacaaaacta
    11161 gcttattttt accaaaagat gaaaaatcaa gaggactatt ttataaagag attaacattt
    11221 gatcaattaa atgaacttgc tgaaaatttt tccgaaaatt gaagctttga ccttgatttt
    11281 tgtttgcctt tttttatata ataatactag caagcgagaa tggagagaga catggcaagt
    11341 attatttttt cagcgaaaga tatttttgat caagagtttg gacgtgaagt ccgtggctat
    11401 aataaagtag aagttgacga gtttttagac gatgtcatca aggactatga aacctatgct
    11461 gccttggtca agtcacttcg tcaggaaatt gcggatttga aggaagaatt aactcgtaaa
    11521 ccgaaacctt caccagttca agcagaaccc cttgaagcgg caattacaag ttctatgacg
    11581 aattttgata ttttgaaacg cctgaataga ttggaaaaag aagtttttgg taaacaaatt
    11641 ttagataact cagattttta agtagttatt tgagatgtgc aatttttgga taatcgcgtg
    11701 aggagaattg cttctcatga ggaaagtcca tgctagcaca ggctgtgatg cctgtagtgt
    11761 ttgtgctagg cgaaaccata agcctaggga cgagaaatcg ttacggcagt tgaaatggct
    11821 aagtccttgg ataagccaga gtaggcttga aagtgccaca gtgacggagt ctttctggaa
    11881 acagagagag tggaacgcgg taaacccctc aagctagcaa cccaaatttt ggtcggggca
    11941 tggagtacgc ggaaacgaac gtagtattct gactgctatc agttagagct gttagtggta
    12001 gacagatgat tatcgaagga agtggtccta gtcacttctg gaacaaaaca tggcttatag
    12061 aaaattgcat ataggttggg gctgagaaat tttctcaacc tcatttttta aagtggacat
    12121 atagaaaggt cttgcaagac tgtaacatga aaaaagaatt taatttaatt gcaactgtgg
    12181 cagcagggct tgaggctgtc gttggtcgtg aagtgcgaga gttgggctac gattgtcaga
    12241 ttgaaaatgg acgtgttcgt tttcaaggag acgtgagagc tattatcgaa accaaccttt
    12301 ggcttcgggc agcagatcgt atcaaaatta tcgtaggaac gttccctgct aagacttttg
    12361 aagagctatt tcagggagtt ttcgctttag attgggaaaa ttatttacca cttggagctc
    12421 ggttcccgat ttcaaaagct aaatgtgtta agtccaaact tcacaatgag cccagtgttc
    12481 aggctatttc taagaaagct gttgtcaaga aattgcagaa acactatgct cgcccagaag
    12541 gggttcctct gatggagaat ggcccagagt ttaagattga ggtctctatt ctcaaagatg
    12601 tggcaactgt catgattgat acgaccgggt ctagcctctt taaacgtggt tatcgtaccg
    12661 aaaaaggtgg cgctcctatc aaggaaaata tggcagcagc cattttacaa ctttctaact
    12721 ggtatccaga caagcctttg attgatccga cctgtggttc ggggactttc tgtattgagg
    12781 cagttatgat tgctagaaag atggcgccag gtcttcgtcg ctcttttgca tttgaggaat
    12841 ggaactggat cagcgatcgc ttgattcaag aagtgcgcac agaagcggct aaaaaagtag
    12901 accgtgagct tgagctggat atcatgggct gtgatattga tgctcgcatg gtggaaattg
    12961 ctaaggccaa tgctcaggca gctggtgttg caggagacat tacttttaag cagatgcgcg
    13021 tgcaggattt acgttccgat aaaatcaatg gagtaatcat ttccaatccg ccttatggtg
    13081 aacgtttgtc agatgatgca ggggtgacca agctctatgc tgagatgggg caagtatttg
    13141 caccgctgaa aacttggagc aaatttatcc tgactagtga tgaagctttt gaaagcaagt
    13201 atggtagcca agcagataag aagcgtaagt tatacaacgg aaccttgaaa gtggatctgt
    13261 atcaatattt tggtcagcgt gtcaaacggc aagaggtaaa atagaaaggg atactcatga
    13321 gtaaaaaaag acgaaatcgt caaaaaaaag aaggtcaaga accgcaattt gattttgatg
    13381 aagcaaaaga gctaacagtt ggtcaagcta ttcgtaaaaa tgaagaagtg gaagcaggag
    13441 tcttgcctga ggattccatt ttggacaagt atgttaagca acacagagat gaaattgagg
    13501 cggataagtt tgcgactcgt caatacaaaa aagaggagtt cgttgaaact cagagtctgg
    13561 atgatttaat tcaagagatg cgtgaggctg tagagaagtc agaagcttct tcggaggaag
    13621 ttccatcttc tgaagacatc ttactaccct tgcctctgga cgatgaggag caaggcttgg
    13681 atcctctatt gctagatgat gaaaatccaa cagaaatgac tgaagaagtg gaagaggagc
    13741 aaaacctttc tcgtctggat caagaggact cagaaaagaa aagtaaaaaa ggctttattt
    13801 tgaccgtttt ggcgcttgta tcagtaatta tttgtgtcag tgcttattat gtctaccgtc
    13861 aagtggctcg ttcgaccaag gaaattgaaa cttctcaatc aactacagcc aatcaatcgg
    13921 atgtggatga ttttaataca ctttatgacg ccttttacac agatagcaat aaaacggctt
    13981 tgaaaaatag ccagtttgat aaactgaatc aactcaagac tttacttgat aagctggaag
    14041 gtagtcgtga acatacgctt gccaaatcta aatatgatag tctagcaacg caaatcaagg
    14101 ctattcaaga tgtcaatgct caatttgaga aaccagctat tgtggatggt gtgttggatg
    14161 ccaatgccaa agccaaatcg gatgctaaat ttacggatat taaaactgga aatacggagc
    14221 ttgataaagt gctagataag gctatcagtc ttggtaagag ccagcaaaca agtacttcta
    14281 gctcaagttc aagtcaaact agcagctcaa gttcaagtca agcaagctca aatatgacaa
    14341 gtgagccaaa accaagtagt tcaaatgaga ctagaagtag tcgcagtgaa gtcaatatgg
    14401 gtctctcgag tgcaggggtt gctgttcaaa gaagtgccag tcgtgttgcc tataatcagt
    14461 ctgctattga tgatagtaat aactctgcct gggattttgc ggatggtgtc ttagaacaaa
    14521 ttctagcgac ttcacgttca cgtggctata tcactggaga ccaatatatc cttgaacgtg
    14581 tcaatatcgt taacggcaat ggttattaca acctctacaa gccagatgga acctatctct
    14641 ttacccttaa ctgtaagaca ggctactttg tcggaaatgg cgctggtcat gcggatgact
    14701 tggactacta agcagtcgtt acaaaattct ttcttttcaa aagtaaaaat gataaaataa
    14761 aacaaatgaa acaagaggag tgtcaaatga caaaagctaa ctttggtgtc gtaggtatgg
    14821 ccgtaatggg tcgtaacctt gcccttaata ttgaatctcg tggttacaca gttgctatct
    14881 ataaccgtag taaagaaaaa acggaagatg tgattgcttg ccatcctgaa aagaactttg
    14941 taccaagcta tgacgttgaa agttttgtaa actcaatcga aaaacctcgt cgtatcatgc
    15001 tgatggttca agctggacct ggtacagatg ctactatcca agcccttctt ccacaccttg
    15061 acaagggtga tatcttgatt gacggaggaa atactttcta caaagatacc atccgtcgta
    15121 atgaagaatt ggcaaactct ggtatcaact ttatcggtac tggggtttct ggtggtgaaa
    15181 aaggtgccct tgaaggtcct tctatcatgc ctggtggaca aaaagaagcc tacgaattgg
    15241 ttgcggatgt tcttgaagaa atctcagcta aagcaccaga agatggcaaa ccatgtgtga
    15301 cttacatcgg tcctgatgga gctggtcact atgtgaaaat ggttcacaat ggtattgagt
    15361 acggtgatat gcaattgatc gcagaaagct atgacttgat gcaacacttg ctaggccttt
    15421 ctgcagaaga tatggctgaa atctttactg agtggaacaa gggtgaatta gacagctact
    15481 tgattgaaat cacagctgat atcttgagcc gtaaagacga tgaaggccaa gatggaccaa
    15541 tcgtagacta catccttgat gctgcaggta acaagggaac tggtaaatgg acgagccaat
    15601 catctcttga ccttggtgta ccattgtcac tgattactga gtcagtgttt gcacgctaca
    15661 tttcaactta caaagaagaa cgtgtacatg ctagcaaggt gcttccaaaa ccagctgcct
    15721 tcaactttga aggagacaag gctgaattga ttgaaaaaat ccgtcaagcc ctttacttct
    15781 caaaaatcat ttcatacgca caaggatttg ctcaattgcg tgtagcctct aaagaaaaca
    15841 actggaactt gccatttgca gatatcgcat ctatctggcg tgatggctgt atcatccgtt
    15901 ctcgtttctt gcaaaagatt acagatgctt acaaccgcga tgcagatctt gccaaccttc
    15961 ttttggacga gtacttcttg gatgttactg ctaagtatca acaagcagtg cgtgatatcg
    16021 tagctcttgc ggttcaagct ggtgtgccag tgccaacttt ctcagcagct attacttact
    16081 ttgatagcta ccgttcagct gaccttccag ctaacttgat tcaagcacaa cgtgactact
    16141 ttggtgctca cacttaccaa cgtaaagaca aagaaggaac cttccactac tcttggtatg
    16201 acgaaaaata agtaggtcag ccatggggaa acggatttta ttacttgaga aagaacgaaa
    16261 tctagctcat tttttaagtt tggaactcca gaaagagcag tatcgggttg atctggtaga
    16321 ggaggggcaa aaagccctct ccatggctct tcagacagac tatgatttga tgttattgaa
    16381 cgttaatctg ggagatatga tggctcagga ttttgcagaa aaattgagtc gaactaaacc
    16441 tgcctcagtc atcatgattt tagatcattg ggaagacttg caagaagagc tggaagttgt
    16501 tcagcgtttt gcagtttcat acatttataa gccagtcctt atcgaaaatc tggtagcgcg
    16561 tatttcggcg atcttccgag gtcgggactt cattgatcaa cactgcagtc tgatgaaagt
    16621 tccaaggacc taccgcaatc ttaggataga tgttgaacat cacacggttt atcgtggtga
    16681 agagatgatt gctctgaccc gccgtgagta tgaccttttg gcgacactta tgggaagcaa
    16741 gaaagtattg actcgtgagc aattgttgga aagtgtttgg aagtatgaaa gtgcgaccga
    16801 gacaaatatc gtagatgtct atatccgcta tctacggagc aagcttgatg ttaaaggcca
    16861 aaaaagctac attaaaaccg tgcgtggtgt tggatatacc atgcaagaat agataagcag
    16921 ttgcagttgt gtaactgctt ttttgaggag tttttatata ttgacatgca gtcgggtctt
    16981 tgctacaatc gttttaggag gatatataga tgaaactttt gaaaaaaatg atgcaagttc
    17041 tactagcagt ctttttcttt ggtttgctag ctacaaatac ggtatttgcg aataccacag
    17101 gtggccgatt tgttgataag gataatagaa aatattatgt aaaagatgat cataaagcaa
    17161 tctattggca taaaatagac ggtaaaactt actattttgg tgatattgga gagatggttg
    17221 tcggttggca atacttagaa attcctggaa caggttatcg tgataattta ttcgataacc
    17281 aaccagttaa tgaaattggc cttcaggaga agtggtacta ttttggacaa gatggtgctt
    17341 tgctagaaca aacagataaa caagtactag aggcaaaaac gtctgaaaat acaggaaaag
    17401 tatacggtga acaatatcct ctatctgctg aaaagagaac ttattatttt gataataatt
    17461 atgctgtaaa gacaggctgg atttatgaag acggcaattg gtattattta aataagctag
    17521 gaaattttgg cgatgattct tacaatccac taccaattgg tgaagttgct aagggttgga
    17581 ctcaagattt tcatgttact attgacattg atagaagcaa acctgctcca tggtactacc
    17641 tagacccaga aactggcatt atgcgaacag gatggcaata tctaggtaat aactggtact
    17701 acctccgttc atcaggagca atggcaacag gttggaaata tgtacgaggc aaatggtatt
    17761 acttagataa taaaaatggt gatatgaaga ctggttggta tcaagatggt tcaacttggt
    17821 actatttaga tccttctaat ggagatatga aaataggttg gacaaaagta aatggaaaat
    17881 ggtattatct caattcaaat ggagcaatgg ttacaggtag tcaaactatc gatggtaaag
    17941 tttataactt cgcttcatct ggtgagtgga tttaatgttg gaggatatat aaaatgaagc
    18001 ttttgaaaaa aactatgcaa gctggactaa cagtaatttt ctttggtttg ctagctacga
    18061 atacagtatt tgcagataac tctgaaggat ggcagtttgt ccaagaaaac ggtagaacct
    18121 attacaaaaa gggggacctc aaagaaacct actggcgagt gattgatggt aagtactatt
    18181 attttgattc tctatctgga gagatggttg tcggctggca atatatcccg tttccatcta
    18241 aaggtagtac aattggtcct tacccaaatg gtatcagatt agaaggtttt ccaaagtcag
    18301 agtggtacta ctttgataaa aatggagtgc tacaagagtt tgttggttgg aaagaattag
    18361 agattaaaac tcaagacagt gttggaagaa agtatgggga aaaacgtgaa aattcagaag
    18421 ataaagaaga gaagcgttat tacacgaact attactttaa tcaaaatcat tctttagaga
    18481 caggttggct ttatgatcag tctaactggt attatctagc taaaacagaa attaatggag
    18541 aaaactatat tggtggtgaa agacgtgcag gttggataaa tgatgcttca gcttggtact
    18601 acctagatcc agcaactggt attatgcaaa caggttggca atatctaggt aataagtggt
    18661 actacctccg ttcctcagga gcaatggcca ctggctggta tcaggaaggt accacttggt
    18721 attatttaga ccacccaaat ggcgatatga aaacaggttg gcaaaacctt gggaacaaat
    18781 ggtactatct ccgttcatca ggagctatgg caactggttg gtatcaagat ggttcaactt
    18841 ggtactacct aaatgcaggt aatggagaca tgaagacagg ttggttccag gtcaatggca
    18901 actggtacta tgcttatagc tcaggtgctt tggcagtgaa tacgaccgta gatggctatt
    18961 ctgtcaacta taatggcgaa tgggttcggt aatgacaggg cgcttataat tatattagaa
    19021 cactcgctta atcaaagaag tgactttgtt gtacttgacc tgacgattgg ggagattaaa
    19081 agcatgtgaa atctacacat gctttttcgt tagttatgag tgttgaactt gactaaccat
    19141 tgacaaaaga taagagcaat caaaataaga tacaaaaata tcaatgctaa ctgcaccgaa
    19201 acgcccgatg ctatggatag ggctaatagg gagagcaggg acggggcgag aatgtctact
    19261 gtgctcagag cagagcccac cgtccccatc ttgtcactgg ggatatgggc aaagacagct
    19321 gcttgtaggc gagggctgag aatgccgata atcgtagaac tgatgaaact ggtcacgaaa
    19381 attacgatat aggcttgaca aaggatacta gttattagag ccaataggga aatctcacag
    19441 aaaaccaaga ggtgcgtgaa acggatattt ttaaatagat tactgctggt catattgcca
    19501 aggatagctc cacaagacat gatgatggag agcagggcga agagttgccc aatgctcagt
    19561 tgtgctagtg tctgttggat gaagcggagg gcaatcagcc gaggggacac atccaagatg
    19621 gcaatcatac tggtcgtcag aaacagtagt atgacggtat cttttaacct caggagcaca
    19681 gccagcgact gtttaagatg ttgaaaatat ttcttaaaac tcagtgctgt atgtgacatt
    19741 tcaatccttt tttcaacctc atacaaggta tgtcgaataa catacaggcc caaaaaggca
    19801 atgacaaaag tcagagtgtt gataagggaa atagtttgaa tacttataac attgataagg
    19861 aatgcaccag ccagattggc gacaatgcgg acaaccctca tcagagactg cctgaacccc
    19921 atggcatcat gcaggtcgtc cttaattaca ctgatgtaga gggcgttcat catgtaggca
    19981 gacaggtagc tcaaggtgtc agagatgagg ttgatgatga caatcataat gactgaaact
    20041 aatgaaaagg gcgacaagac gagaaatact gtcaaaatag cgtagagcag gattttgatt
    20101 ttggcaatac agagtgcgtg tttgactcta ttttcttgaa aatctcctag aatcccgaga
    20161 aagagttgcg atagtagggg aaccatctct gaaatggaaa tcagtaaaat tgctagcgga
    20221 gcgaaattgt actgagccac ataattgata atggcgaggt agaaaatact gtcgccgaat
    20281 gatgaaaaac agctgtttag cagcaactgt ctatatatgt tgtttgtcca aaataatttc
    20341 atacttttct ccttatgtct gcacatcgaa tgggaattta ttatttaata gacaccaata
    20401 tacccccatg cttcctattc cgaagtcaaa caaatctact ttaccactat ctccttgaaa
    20461 catcagtaaa ccgtgatcat ctcgttcaga acatgctaaa attacctgtt ggattttttt
    20521 catcagtaat ttgttttgat tgtagacagt ggtttgtagt aagctagtta ttccgtggca
    20581 atatcctgtc atcattttta gatgatggtt aaaaacaaat tcttgatatt tactaataat
    20641 atctttgttt ccgtcacaat cgtacataca aagatataag attattccgg aaattccttc
    20701 gcaccaagaa agttgtaaat ttcctaaatt ttcagtatta gaagtaactt tttctaataa
    20761 tttttttaat tcagcatgga atgtatggag atcattatag aacatagaag gttctaagac
    20821 tttagaatag gcaaataaag catatgcaat tccacaatat ccatgagcaa agctatagtc
    20881 taaaatgctt tctttggctg tatctctttg taggaaataa tgataaattt ccccatatac
    20941 tattgtggat gaaagtttta agagtattgt cattggtaaa ttgatagtat tttattaatg
    21001 ataataggac accagatttt cccaatgcaa agtctatttc ctctagagta tcataattct
    21061 ctattaattt cttagcggtg acgtttgcta attgtagata gtattgattt ttactgatag
    21121 aaaataaatg taggagtaac catagatagc ctgcatcacc aaatagcagt gagtattgat
    21181 attcatatgt gtgtgtcgtt ctaattgtca ctgccttctt gatgatagag agtattttag
    21241 aatccagttc tggggggtac aatttattta gaggaaacag tagtccagct attccgtgtt
    21301 ggaatgataa attacttacg aaatttccaa attctgttga agaagtgatc cttccttcag
    21361 aatttacagt tttatctagt aaaaatcgta gaacactatt acattctgtt ttgtaatcat
    21421 atttatgttc ctctattttt ttaattttga ctaaattatt tgtttgactt aaatttagta
    21481 gatattgtaa ccaaaatagt tgctcatctg tgagctgacc ttgagtatgt gatagttcta
    21541 gtaattgaat aggatccttt aggtagattc aatcaatcta taacacaaca acataataat
    21601 acttttagga taacttgtca aatattatag taaaatgaaa taataacagg acaaattgat
    21661 caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
    21721 caatatacta tagtatatat ttttacatct tgaattgtca aattaagtgc ataactttcc
    21781 cgtagcatac ttcataaggc gttttccact taagaaattt tcgtggtcga ttatttagaa
    21841 cctgtattca caaaaaaatg ttaaactaga gttatgacac gcaaagcata tgatactgat
    21901 ttaaacgatc aagaatgggc taagattgaa ccctatttct ccaaacaccg cacctataaa
    21961 tggccgaaac gagtacttgt taatgagact ttgtacgtca ctaaaacaag ttgtcaatgg
    22021 cgcatgctcc ctcacgactt tcctctttac ttaatggtat ggagtttctt tcgtcgttct
    22081 atgacgacag gttggttcca ggtcaatggc agatggtact acgcttatag ctcaggtgcc
    22141 ttggcagtga atacgaccgt agatggctat tctgtcaact ataatggcga atgggttcaa
    22201 taatgaaaga agcgattgtg aatggaaaca atcgcttttt ttgtgaaaat ataataaaat
    22261 agataggaaa gaattaatac ttgtatgaaa aataagacgt tttttcgtct agtaaaagga
    22321 aaaaatgaca aaaaaagttg gtgtcggtca ggcacatagt aagataattt taatagggga
    22381 acatgcggtc gtttacggtt atcctgccat ttccctgcct cttttggagg tggaggtgac
    22441 ctgtaaggta gttcctgcag agagtccttg gcgcctttat gaggaggata ccttgtccat
    22501 ggcggtttat gcctcactgg agtatttgga tatcacagaa gcctgcattc gttgtgagat
    22561 tgactcggct atccctgaga aacgggggat gggttcgtca gcggctatca gcatagcggc
    22621 cattcgtgcg gtatttgact actatcaggc tgatctgcct catgatgtac tagaaatctt
    22681 ggtcaatcga gctgaaatga ttgcccatat gaatcctagt ggtttggatg ctaagacctg
    22741 tctcagtgac caacctattc gctttatcaa gaacgtagga tttacagaac ttgagatgga
    22801 tttatccgcc tatttggtga ttgccgatac gggtgtttat ggtcatactc gtgaagccat
    22861 ccaagtggtt caaaataagg gcaaggatgc cctaccgttt ttgcatgcct tgggagaatt
    22921 aacccagcaa gcagaagttg cgatttcaca aaaagatgct gaaggactgg gacaaatcct
    22981 cagtcaagcg catttacatt taaaagaaat tggagtcagt agccctgagg cagacttttt
    23041 ggttgaaacg actcttagcc atggtgctct gggtgccaag atgagcggtg gtgggctagg
    23101 aggttgtatc atagccttgg taaccaattt gacacacgca caagaactag cagaaagatt
    23161 agaagagaaa ggagctgttc agacatggat agagagcctg taacagtacg ttcctacgca
    23221 aatattgcta ttatcaaata ttggggaaag aaaaaagaaa aagagatggt gcctgctact
    23281 agcagtattt ctctaacttt ggaaaatatg tatacagaga cgaccttgtc gcctttacca
    23341 gccaatgtaa cagctgacga attttacatc aatggtcagc tacaaaatga ggtcgagcat
    23401 gccaagatga gtaagattat tgaccgttat cgtccagctg gtgagggctt tgtccgtatc
    23461 gatactcaaa acaatatgcc tacggcagcg ggcctgtcct caagttctag tggtttgtcc
    23521 gccctggtca aggcttgtaa tgcttatttc aaacttggat tggatagaag tcagttggca
    23581 caggaagcca aatttgcctc aggctcttct tctcggagtt tttatggacc actaggagcc
    23641 tgggataagg atagtggaga aatttaccct gtagagacag acttgaaact agctatgatt
    23701 atgttggtgc tagaggacaa gaaaaaacca atctctagcc gtgacgggat gaaactttgt
    23761 gtggaaacct cgacgacttt cgacgactgg gttcgtcagt ctgagaagga ctatcaggat
    23821 atgctgattt atctcaagga aaatgatttt gccaagattg gagaattaac ggagaaaaat
    23881 gccctggcta tgcatgctac gacaaagact gctagtccag ccttttctta tctgacggat
    23941 gcctcttatg aggctatgga ctttgttcgc cagcttcgtg agaaaggaga ggcctgctac
    24001 tttaccatgg atgctggtcc caatgttaag gtcttctgtc aggagaaaga cttggagcat
    24061 ttatcagaaa ttttcggtca gcgttatcgc ttgattgtgt caaaaacaaa ggatttgagt
    24121 caagatgatt gctgttaaaa cttgcggaaa actctattgg gcaggtgaat atgctatttt
    24181 agagccaggg cagttagctt tgataaagga tattcccatc tatatgaggg ctgaaattgc
    24241 tttttctgac agctaccgta tctattcaga tatgtttgat ttcgcagtgg acttaaggcc
    24301 taatcctgac tacagcttga ttcaagaaac gattgctttg atgggagact tcctcgctgt
    24361 tcgcggtcag aatttaagat ctttttccct aaaaatctgt ggcaaaatgg aacgagaagg
    24421 gaaaaagttt ggtctaggtt ctagtggcag cgttgttgtc ttggttgtca aggctttact
    24481 ggctctctat aatctttcgg ttgatcagaa tctcttgttc aagctgacta gcgctgtctt
    24541 gctcaagcga ggagacaatg gttccatggg cgaccttgcc tgtattgtgg cagaggattt
    24601 ggttctctac cagtcatttg atcgccagaa ggtggctgct tggttagaag aagaaaactt
    24661 ggcgacagtt ctggagcgtg attggggatt ttttatctca caagtgaaac caactttaga
    24721 atgtgatttc ttagtgggat ggaccaagga agtggctgta tcgagtcaca tggtccagca
    24781 aatcaagcaa aatatcaatc aaaatttttt aacttcctca aaagaaacgg tggtttcttt
    24841 ggtcgaagcc ttggagcagg ggaaagccga aaaagttatc gagcaagtag aagtagccag
    24901 caagctttta gaaggcttga gtacagatat ttacacgcct ttgcttagac agttgaaaga
    24961 agccagtcaa gatttgcagg ccgttgccaa gagtagtggt gctggtggtg gtgactgtgg
    25021 catcgccttg agttttgatg cgcaatcaac cgaaacctta aaaaatcgtt gggccgatct
    25081 ggggattgag ctcttatatc aagaaaggat aggacatgac gacaaatcgt aaggacgagc
    25141 atatcctcta tgcccttgag cagaaaagtt cctataatag ctttgatgag gtggagctga
    25201 ttcattcttc cttgcctctt tacaatctgg atgaaatcga tctttcgaca gagtttgctg
    25261 gtcgaaagtg ggactttcct ttttatatca atgccatgac tggtggaagt aataagggaa
    25321 gagaaatcaa tcaaaagctg gctcaggtgg cggaatcttg tggtatttta tttgtaacgg
    25381 gttcttatag cgcagccctc aaaaatccaa cggatgattc tttttctgtc aagtctagtc
    25441 atcccaatct cctccttgga accaatattg gattggacaa acctgtcgag ttaggacttc
    25501 agactgtaga agagatgaat cctgttctat tgcaagtgca tgtcaatgtc atgcaggaat
    25561 tactcatgcc cgagggagaa aggaagttta gaagctggca atcgcatcta gcagattata
    25621 gcaagcaaat tcccgttcct attgtcctca aggaagtggg ctttggaatg gatgccaaga
    25681 caatcgaaag agcctatgaa ttcggtgttc gtacagtgga cctatcgggt cgtggtggca
    25741 ccagctttgc ctatatcgaa aaccgtcgta gtggccagcg tgattacctc aatcaatggg
    25801 gtcagtctac catgcaggcc cttctcaatg cccaagaatg gaaagataag gtcgaactct
    25861 tggttagtgg aggggttcgg aatccgctgg atatgattaa gtgcttggtt tttggtgcta
    25921 agtctgtggg attgtcacga accgttctgg aattggttga aacctacaca gttgaagaag
    25981 tgattggcat tgtccaaggc tggaaagcag acctacgctt gattatgtgt tcccttaact
    26041 gtgccaccat agcagatcta caaaaagtag actatcttct ttatggaaaa ttaaaagaag
    26101 caaaggatca gatgaaaaag gcgtaaccag cgcctttttt ccatcctcag actgaggtga
    26161 cttttttgaa ttgtgataaa atagaaggga gaggatgaac ctatgagaaa atttaaaatc
    26221 tttttattta tcgaagcctg tcttctgaca ggagctctga ttttgatggt atcagagcat
    26281 ttttcgcgtt ttctgctgat actattcctc tttttgcttt tgattcgcta ctacactggt
    26341 aaagagggaa ataatcttct tttagtagcg gcaaccattc tcttcttttt catcgttatg
    26401 ctcaatcctt ttgtgattct agctattttt gttgcggtta tctatagcct ctttcttctt
    26461 tacccgatga tgaaccagga aaaagagcag accaatttgg tttttgaaga ggtcgtgacg
    26521 gttaagaagg agaaaaatcg ttggtttgga aatcttcatc atttttcaag ctaccagact
    26581 tgccaattcg atgatatcaa tctctttcgc ttcatgggca aggacactat tcatctggag
    26641 agggtcatct taaccaatca tgacaatgtc attatcctca gaaagatggt aggaacgacc
    26701 aaaatcatcg tacctgtaga tgtggaagtc agtctcagcg ttaactgtct ctatggggat
    26761 ttgacttttt tcaaccagcc caagcgagcc ctccgcaatg aacactatca tcaagaaaca
    26821 aaagactatc tcaagagtaa caagagtgtc aagattttct tgaccactat gattggtgat
    26881 gtggaggtgg ttagaggatg aaaaaacaag cctatgtcat tattgctctc acctccttcc
    26941 tgtttgtctt ttttttctcc cacagcttgc tggaaatact tgattttgac tggtctatct
    27001 ttttgcacga tgtcgaaaaa acagaaaaat ttgtcttttt attgttggtt ttcagcatgt
    27061 ccataacctg tctcttagcc ctgttttggc gagggatcga agagctttct ctaagaaaaa
    27121 tgcaggctaa tctcaagcgt ttattggcag ggcaagaagt ggttcaggtt gcagatccag
    27181 atttggatgc cagtttcaag tccttatcag gtaaacttaa ccttttgaca gaagctcttc
    27241 aaaaagctga aaatcatagc cttgctcagg aagaggaaat catcgagaaa gaacggaagc
    27301 gaattgctcg ggatttgcac gatacagtca gtcaggagtt gtttgcggcc cacatgattt
    27361 tatcgggtat cagtcagcag gctttgaaat tggatagaga aaagatgcag atccagttgc
    27421 agagtgtcac agctatttta gaaactgccc agaaggattt gcgggttttg ctcttgcatt
    27481 tgcgaccagt tgaactggag cagaagagct tgatagaagg gattcaaatt ctcttaaaag
    27541 agcttgagga caagagtgat cttagggtta gtctcaagca gaatatgacg aaattgccta
    27601 agaaaatcga ggagcatatc ttccgtatcc tgcaagagtt gattagcaat accctccgcc
    27661 atgcccaggc atcttgccta gatgtctacc tctatcagac agatgttgaa ttgcaactga
    27721 aggtggtgga caatgggatt ggtttccggt taggaagctt agacgacttg agttatggac
    27781 tgcgaaatat caaggagagg gttgaagata tggctggaac agttcaactc ttgacagctc
    27841 ccaagcaagg gctggcagtt gatatccgta ttcccctgtt agataaggaa tgataaagga
    27901 gtaaagatga aaattttact agtagatgac catgaaatgg tccgtttggg tttgaaaagc
    27961 tactttgacc tccaagacga tgtagaagtt gtgggtgagg cgtccaacgg gtctcaaggg
    28021 attgacttgg ccttggaact gcgtccagat gtcattgtca tggatattgt catgcctgag
    28081 atgaatggga ttgacgcgac cttagcaatc cttaaagaat ggcctgaagc caagattttg
    28141 attgtgacct cttatttgga caatgaaaaa atcatgccag tcttagatgc tggtgccaaa
    28201 ggctatatgc tcaagacttc tagtgcagat gaattgcttc atgccgtcag taaggtagct
    28261 gctggcgagc tggccattga gcaagaggtt agcaagaagg ttgaatacca ccgcaatcat
    28321 atggaacttc atgaagaatt gactgcgcgt gagcgagatg ttctccaact catcgccaag
    28381 ggctacgaaa atcagcgcat cgcagatgac ctctttatct ctctcaagac ggtcaagacc
    28441 cacgtgtcca acattcttgc caaacttgaa gtcagcgatc gtacccaggc ggctgtctat
    28501 gcctttcagc accatttggt ggggcaagat gagttttaga tgagtttagc agatttactt
    28561 gaggagctag aagcagcaaa agactctaaa aaagcaaggt ccatggaagc ctatatgcgc
    28621 catcaatttt cttttctagg tattgcggtg cctgaaagaa ataaactcta taaaaatatt
    28681 tactagaagc gaaaaaaaca aagattatcg attgggattt tgcagacact tgctgggaaa
    28741 aggagcctag aaaataccaa tatgtggctg ctaactattt gaaaacaatg cagtcttatc
    28801 taaaggaaac cgatttgcct aagctagagc agttagtcgt gacaaaatcc tggtgggaca
    28861 cggtagatat cctagatcga gtagtaggga gtttggtgta tgaacaccct gaactagaag
    28921 aaataatctt aatactcttc gaaaatctct tcaaaccacg tcagctctat ctgcaacctc
    28981 aaaacagtgt tttgagcaat ctgcggctag cttcctagtt tgctctttga ttttcattga
    29041 gtataaaatg gagcctatca gataatatct ggctgagacg tgtcgctatt gaccaccagt
    29101 tgttaagaaa agagaagacc aatactcaat taatggaaaa gattctactt cataacttga
    29161 accaaacaga attttttatc aataaagcca ttggctggac tctgcgagac tactccaaaa
    29221 cgaatcccac ttgggtaaca tgctttattg agaaaaacaa ggaaagaatg gctgaactta
    29281 gtatcaaaga agcaagtaag tacctctagc gtgattgaaa agcgcagtca ttaattgaaa
    29341 agtttacctg tttatgtgat aatggagggt atttaaagaa ggtggtagat ttcttgattc
    29401 tatagtagat taagaaattt agaatagtac gctataatta cattaggagg atacaataat
    29461 gaagaaaact gtttataaaa aattgggtat ttcaattatt gcgagtactt tattggctag
    29521 ccagttatcg acagtatctg ctttgagtgt tatttctagt acaggtgaag aatatgaggt
    29581 aagtgagaca ctagaaaaag gtccagggtc taatgattct tcattatctg agatttcacc
    29641 aacgtatggt tcatactacc aaaagcaatc agaagtatta tcggtaatga tgatttgatt
    29701 aaaattgata atactttaca gtatccgtat tcgacttcag ctatggtttt atctaagtat
    29761 tatggagtag ccgatggaat gaatgtagaa gggaggggaa gtgcgaattt tattaaagat
    29821 aatgtgttaa ttacagcggc tcacaactac tacagacatg actatgggaa agaagcggat
    29881 gatatttatg ttcttccggc tgttagtcca agtcaagaac tatttggaaa gatcaaagta
    29941 aaggaagttt gttatttgaa ggaatttaga aatttaaatt ctaaggatga aagggaatat
    30001 gacttggctt tattaattct agaagagccc attggtgcaa aattagggac tttgggtttt
    30061 cctactagtc aaaaaaattt gacaggaata actgtgacta tcacaggcta tccatcatat
    30121 aattttaaaa ttcatcaaat gtatacagat aaaaaacaag ttttaagtga tgatggcatg
    30181 ttcttggatt accaagttga tactttagag gggtctagtg gatctacagt ttatgatgct
    30241 agtcaccgtg tagtaggagt gcatacttta ggagatggag ctaatcaaat taacagtgca
    30301 gttaaattaa atgaacgaaa tttgtcattt atttattcgg ttcttaaagg ttactctctt
    30361 gaaggatggt gaatgggtta ggtaatgaag gctaattgta aactgtgatg gatacttaac
    30421 tttgtataat aggtggataa aagtctttaa aataggaaaa aacgcatatt attaggtgtt
    30481 taaaaacctt gataatatgc gttttataat ggaaagattt cttcaatttc gtcttgtata
    30541 gtagattgaa actataatag tacacctcta cttctaaaac attgttagaa attgatttga
    30601 ctgtcctgat cgatttgtcc tgttcttatt tcgttttact ataatgtaat cgtccaataa
    30661 cgaagtgttt ttggatagtc cgaaaagttt agcttcatat agagtaattg gtattagtga
    30721 tagagtgtta gaaattttaa aagaatatct tcttttagaa gaaggtgatt atatatttac
    30781 aaataatggt tctcccttaa tgataacttg tttcaattac tttttgaaaa acaggtttag
    30841 gaaaagtgaa attaagaaag atgattttgt attgactgct catgttttta gatattcgca
    30901 tataagtttg cttgctgaat tggaagtacc tataatagcg ataatggatc gtgtagatta
    30961 tactaatgaa acaaagattt taagtgttta tactcatgtt actgagaaga tgaagtcaaa
    31021 tataacttaa aaattagata aattagtttt ggaaaatgac taaccaaaag atatccaaag
    31081 tagtctaaaa ttgtctatac tttatgagtg ttttagttag gaaaaaggct tgttgtctat
    31141 aattgtctgc attagtctag attttattta tagaaaatgt tataatagac tgtatttaaa
    31201 aaattttaag gagaaatgac agaatgtctg tatcatttga aaacaaagaa acaaaccgtg
    31261 gtgtcttgac tttcactatc tctcaagacc aaatcaaacc agaattggac cgtgtcttca
    31321 agtcagtgaa gaaatctctt aatgttccag gtttccgtaa aggtcacctt ccacgtccta
    31381 tcttcgataa aaaatttggt gaagaatcac tttaccaaga cgttatgaac gctcttttgc
    31441 caaacgctta tgaagcagct gtaaaagaag ctggtcttga agtggttgcc caaccaaaaa
    31501 ttgacgtaac ttcaatggaa aaaggtcaag actgggttat cgctgctgaa gtcgttacaa
    31561 aacctgaagt aaaattgggt gactacaaaa accttgaagt atcagttgat gtagaaaaag
    31621 aagtaactga cgctgatgtt gaagagcgta tcgaacgcga acgcaacaat ctggctgaat
    31681 tggttatcaa ggaagctgct gctgaaaacg gcgacactgt tgtgatcgac ttcgttggtt
    31741 ctatcgacgg tgttgaattt gacggtggaa aaggtgaaaa cttctcactt ggacttggtt
    31801 caggtcaatt catccctggt ttcgaagacc aattggtagg tcactcagct ggcgaaaccg
    31861 ttgatgttat cgtaacattc ccagaagact accaagcaga agaccttgca ggtaaagaag
    31921 ctaaattcgt gacaactatc cacgaagtaa aagctaaaga agttccagct cttgacgatg
    31981 aacttgcaaa agacattgat gaagaagttg aaacacttgc tgacttgaaa gaaaaatacc
    32041 gcaaagaatt ggctgctgct aaagaagaag cttacaaaga tgcagttgaa ggtgcagcaa
    32101 ttgatacagc tgtagaaaac gctgaaatcg tagaacttcc agaagaaatg atccatgaag
    32161 aagttcaccg ttcagtaaat gaattccttg ggaacttgca acgtcaaggg atcaaccctg
    32221 acatgtactt ccaaatcact ggaactactc aagaagacct tcacaaccaa taccaagcag
    32281 aagctgagtc acgtactaag actaaccttg ttatcgaagc agttgccaaa gctgaaggat
    32341 ttgatgcttc agaagaagaa atccaaaaag aagttgagca attggcagca gactacaaca
    32401 tggaagttgc acaagttcaa aacttgcttt cagctgacat gttgaaacat gatatcacta
    32461 tcaaaaaagc tgttgaattg atcacaagca cagcaacagt aaaataatct taataaacag
    32521 aaaacccacc tgaattggtg ggttttctga tgtactattt tccaaaaatc tctttgaggt
    32581 ctgtgtctgt aatcccaatc atggctggaa tgctgtccca gttttcttcg gttaggatgt
    32641 aggattgttc agaggcactt gatgtgactg tttcagagac agcttgttgc ttttcttcaa
    32701 cattctccag tagatcactg aagcgttcaa tcagataggt ttttcgggca gttccgatgt
    32761 gttgggtagc atagtcgaag gcttgtaatt cgcctagtaa gatgagtttg cttttggcac
    32821 gtgtaatggc tgtgtagatg agatttcgct ccagcatacg tcggctagca ctagtaatcg
    32881 gtaggatgac aactgggaac tcacttccct gagacttatg aatactcatg gcataggcca
    32941 agcgaatctt gtaccattcg ttacgggggt aagagacttc attaccatca aaatcaatga
    33001 caatctcgtc ttgtttcgat tcggtgtatt taccaggaat caggtctgtg atagctccta
    33061 aatccccatt aaagacattg atttcagcat cgttaaccaa atgaatgacc ttgtctccct
    33121 tacgatagtg gcactgagga gcttcaaaac tgagttgatc tttttgtggg ggattgagca
    33181 ggtcttgcat gagctgattg atagcatcaa tccctgccgt ccctcggtac ataggagcca
    33241 gaacttggat atcacgggca ggaataccat ttctgagggc ggcacctaag attttttcaa
    33301 tggtggcagg aatatggcca ctagcaattt caaagtagga acggtcagct tttttttggg
    33361 tgaaatcagc tggcaagatg ccctgtcgaa tctgactagc tagggtgacg atggttgatt
    33421 cttcgctttg tcgataaatt ttttccaagc gagtctgagg aatcaaagga atatgaagca
    33481 gatccgctag aacctgtcca ggactgacag aaggtagctg atcactgtca cctacgatga
    33541 ggatcttact gttagaagag atattggaga agagttgatt ggccagccaa gtatccacca
    33601 tagagaattc atccacgatg ataaagtcag catctaggta atcttccaga tgactggtat
    33661 catcgtcacc tgtcattccc aagtggcggt gtatggtcgc gctaggcaaa cctgtcaatt
    33721 cattcatgcg acgagcagct cgaccagttg gagcagcaag aagaatgggc aggttgcttt
    33781 tcttcctgaa gtcaagtccc tctaacaggg cgtaaacagc gatgatccca ttgataacag
    33841 ttgtcttacc agtaccaggt ccacctgtca ggataaagac cttattctgg atagcatcac
    33901 agatagcctg tttttgaatg ttatcatact caattccaag ctcttgctcg atagtagtga
    33961 tatgtttttg aatggttttt aaatcatgac tcttctgttt tcctttttca aggatacgaa
    34021 ccaagtgact gcggatgcct tcctcagcga aaaagaggct gttgtcaaag atcttggtat
    34081 caatctgctg aaccttgtct tcttcgatca ggtaggagag ctcttgggca acttggctgg
    34141 ggtctagttc cacgggacgg gaagactcaa ggagagtaag ggtttgttcc agcaaatccc
    34201 gtgcttcaac ataggtgtcc cctgtttcca tacaggcctg aaaaagactg tgaactagac
    34261 cggcgcggaa gcgttcagga gcctgacttt cgatgcctag ttcctcagct agttggtcag
    34321 caatggtaaa gcccaaaccc ttgatatcct caaccagctg gtagggataa ttttcaacca
    34381 catcaagggt ttcttccttg taaaagtctt gaatctgaaa ggctagtttg ttgggaatgc
    34441 cgtagttggc tagtttggcc aaaaccatct ccgttccgta gttgagacgg agagtggaga
    34501 cgaaagcctc gcgatttttg gcagagagtc ctgcgatgcc ttctaacttt tctgggtgtt
    34561 gcaaaatttc gtcaatggta ttttcgccat aggtatccac gattttctga gctgtcttga
    34621 gaccaatccc cttgaaatgg ctacttgaaa agtacttgac caagccctta ctagttggtt
    34681 ttgcgcgatc ataacgactg atttgcagtt gttctccata cttggagtgc tggacaattt
    34741 gcccccaaaa agtatagtct tcgccctcaa ttacatcagc catggttcct gtgacaatga
    34801 tttcaaagtc atcaaaatcc tctgcgtccg tatcgtcgat ttctaggagg aggatgcgat
    34861 aaaaattgct gggattttca aaaataatcc gttcaatggt tcctgaaaaa taaacttcca
    34921 taaaattcct ttgcatgaat aggtgagagt tgaggtgttt ctgttctggt aagtttagat
    34981 agttaccaat cattttctca cgatagaaga agaggctgag attggtgatt ctcggcctct
    35041 taggtttctt aaaatgttcc gatacgggtg attggccata agcggaattt agcttcccct
    35101 gtgatatctt ttgctttgaa ggtacctacg tggcggctgt cgctcgaaac caagcggtca
    35161 tctccgagga gaaggtattc tccttctgga acagtaaagc taaagttggt gttgtagttg
    35221 acatcaactg tgaaggcttg agctttttga gcgatacttc taaagaaagt tcctttattt
    35281 ccttcaaagc ccttgcctga gtaagtgctt tggagtttgt catccttgaa gcgtttgata
    35341 tagtctgcta gataaggctc gtccgtttct ttgtcattga tgtagagttt atcattttcg
    35401 taacgaatgg tgtcgccagg cattccaatc acgcgcttga cgatgtcctt attcccatct
    35461 tcctcatggg ccaccacgat atcaaaacgg tcaataggaa ggtgttttac aacgaagaga
    35521 atttcgccat ccgctagggt cggatccatg gaatgtcctt ctacgcgaac attgctccaa
    35581 aaaaagatac gacttaaagc tagtaatgac agaattagga ggaacaatcc ccactctttt
    35641 aagaaatttt taaatgaatt cataacttac ctttctaagc gttttttcgc tttttcagtg
    35701 tttttaaagt gcaatttggc gcagaagctg agtccctgca taccataggc ttgcaaaatc
    35761 tggctagcca ccttgtcaga agccgttcca gctccacttg gaagctgata acccagttct
    35821 cgtcccaaat tttcaagatt ttccagaaag agatcacgcg taatgacaga agaaactgcg
    35881 acagccaagt atttgccctc agccttttct tctaagctga taggattgct gaaacgattg
    35941 gcctcttgtg ccaagtactt gtcataattt ttagcactgg taaaggcatc aatcacaatt
    36001 ttctcaggct gaacaccttt ttgaaggagg agatagatag cctgattatg gagggcaacc
    36061 ttaaccgaaa cagcattgta gcggtctccg atgacctcgt tgtacttgct gggtgagaga
    36121 aggagtgcct ggtgctggat tttttccttg agaataggag caatctgacg gatcttttgg
    36181 tcggtcagag tcttagaatc ccccacaccg agttttcgta aaaagtcgtg ctggtcaggt
    36241 gtgacaaagg cagccacaac tgcaagccca ccaaagtagg aaccatttcc cacctcatct
    36301 gtcccaatta aaggaagatt ttgtccgctg gtttgctcta cagcttgata gccaaagaaa
    36361 ctggcgtatt tttcagcccc ttcaccctga agcaagattt ttccagaagt atagatagaa
    36421 accgttgctt gaggtagttt caaaaagtag cggatatagg gattcttgct aggagccaga
    36481 ctggtttgat agtgttcaag aaaagcctga atatcctttt cgcttggtgt gagtgtgata
    36541 cttgccatag tttctattgt accacaaaag cagtaaaatt tgtaaaaact gacaaaatta
    36601 gcgaattttg gtataatatc gtgaggtgaa ttttatggca aatctaaatc gattcaaatt
    36661 tacattcggg aaaaaatcgt taaccttgac aagcgaacat gataaccttt ttatggagga
    36721 aatcgctaag gttgcgacag aaaaatacca agcaattaaa gaacaaatgc ctagcgcaga
    36781 tgatgaaaca atcgctcttt tgttggcagt caactgttta tcaactcagc tcagccgtga
    36841 gattgaattt gacgataagg agcaagagct agaagaactc cgtcacaagc ttgtgacttg
    36901 taagcaagaa cagagcaaga ttgaggattc cttatgattt cattccttct tctattggtc
    36961 ttggtttggg gattttatat cggctatcgg agaggcctgc tcttacaggt ttattacctg
    37021 atttcagcca tggcatcggc ttttatggct ggccagtttt ataaggggct tggagagcaa
    37081 ttccatttat tgctccctta tgcaaattcg caggaaggtc aggggacttt ctttttccca
    37141 tcggatcaac tctttcagct ggataaggtc ttttatgcag gcatcggcta cttgcttgta
    37201 tttgggattg tctatagcat tggtcgtttg cttggtcttc tcttacactt gattcctagc
    37261 aaaaaactgg gtggtaagtt gttccaagtt tcagcaggta tcttgtccat gttggtgacc
    37321 ttatttgtct tgcaaatggc cttgacaatc ttggcgacca tccccatggc agttatacaa
    37381 aatcctcttg aaaagagtat cgtcgcaaaa cacatcatcc agagcatacc gataacaacc
    37441 agttggctca aacaaatctg ggtgacaaat ttaatcggat aaaaagggca ggagttttcc
    37501 tagccctttg tttacagatt tgactcgaat ctatcagaat gtaaaaagct accacaccta
    37561 gacattcaaa gacaaggaaa taaagatgaa taagaaaata ttagaaacat tagagttcga
    37621 taaggtcaag gccttgtttg agcctcattt gttgaccgag cagggcttgg agcaattgag
    37681 acagctgggt ccgactgcca aagcagataa aatcaaacag gcttttgctg agatgaagga
    37741 aatgcaggct cttttcgtcg agcaaccgca ttttactatt ctctcaacta aggaaattgc
    37801 aggagtctgc aagaggttgg agatgggagc ggctctcaat atcgaggagt tcctactctt
    37861 gaaacgcgtg cttcttgcca gccgagaact tcaaagtttt tacgccaatc tggaaaatgt
    37921 cagcttggaa gaattagccc tttggtttga gaaattacat gattttccgc aattacaagg
    37981 aaatcttcag gcctttaatg atgcgggttt cattgaaaat tttgccagtg aagaattggc
    38041 gcgaatccgt cgaaaaatac atgatagcga gagtcgggta cgcgatgttt tacaagactt
    38101 gctcaagcaa aaagcgcaga tgttgacaga aggaattgtt gctagcagaa atggccgtca
    38161 ggttttacca gtcaaaaaca cctaccgcaa taagattgca ggtgtcgttc atgatatttc
    38221 tgctagtgga aacaccgtct atatcgaacc acgtgaggta gtcaaactga gcgaagaaat
    38281 tgctagtctg cgagcagatg agcgctatga aatgcttcgc attctccaag aaatttctga
    38341 gcgtgtccgc cctcatgcgg ctgagattgc caatgacgcc tggattatcg gtcatctaga
    38401 cttgattcgt gccaaggtcc gctttatcca agaaaggcaa gcagtcgtac ctcagctgtc
    38461 agaaaatcaa gagattcaac tgctccatgt ctgccatcct ttggtcaaaa atgctgtcgc
    38521 aaatgatgtc cattttggtc aagatttaac agctattgtc attacaggtc ccaatacagg
    38581 tggtaagacc atcatgctta aaactctcgg cttgacacag gtcatggctc agtccggttt
    38641 gccgatttta gcagataagg gaagtcgtgt tggtatcttt gaagaaatct ttgccgatat
    38701 tggagatgag cagtctattg agcagagctt gtctaccttc tctagccaca tgaccaatat
    38761 cgtggatatt cttggcaagg tcaatcaaca ttcactctta ctcttggatg agttgggggc
    38821 tggtacagac cctcaagaag gagctgctct tgccatggct attctggagg accttcgcct
    38881 gcgtcaaatc aagaccatgg cgactaccca ctatccggaa ctaaaggctt acggtattga
    38941 gacagccttt gtgcaaaatg ctagtatgga gtttgatact gcaactcttc gcccgactta
    39001 tcgctttatg cagggtgttc ctggccgaag taatgccttt gaaattgcca aacgtctagg
    39061 cctatctgaa gttatcgtag gggatgccag ccagcaggtc gatcaggaca atgatgtcaa
    39121 tcggattatt gaacaattgg aagagcagac gcttgaaagt cataaacgct tggacaatat
    39181 ccgtgaggtg gagcaagaaa atctcaagat gaaccgtgct ctcaaaaaac tttacaacga
    39241 actcaatcgt gaaaaggaaa ccgagcttaa taaggcgcgt gaacaggctg ctgagattgt
    39301 ggacatggcc ctcagtgaga gtgaccagat tctcaaaaat ctccacagta aatctcaact
    39361 caagccccac gaaatcattg aagccaaggc caagctgaaa aaattggctc ctgaaaaagt
    39421 agatttgtct aaaaacaaga tccttcaaaa ggccaagaaa aaacgagctc caaaggtggg
    39481 agatgatatc gtggttctca gttatggcca gcgtggtacc ctgaccagtc aactcaagga
    39541 cggccgctgg gaagcccaag ttggcttgat taagatgacc ctggaagaga aagagtttga
    39601 ccttgttcaa gttcagcaag aaaagccagt taagaagaaa caagtcaatg ttgttaaacg
    39661 gacatctggc cgaggtccac aagccagact ggaccttcga ggcaaacgct atgaagaggc
    39721 catgaatgag ctagatgcct ttatcgacca agccctgctt aacaatatgg ctcaagttga
    39781 tatcattcat ggtatcggaa caggtgtcat ccgtgaagga gtcaccaaat acctacaaag
    39841 aaacaaacat gtcaagagtt ttggctatgc cccacaaaat gctggaggca gtggcgcgac
    39901 tattgtcact tttaaaggat aagaaaagcc tgaggaaata tgtggttcct caagcttttt
    39961 tattttctag gttagacatc taaaggttgt ccagtgtgat tgagttgtgt gacagtaaca
    40021 aagaaatcct ctctcattcg agtcagaagt tgatttggat agaccttttc aaagttatca
    40081 agactggcaa agatatgttt agcaagctct agacctctga catcatcttt tctgagaaaa
    40141 ttattagccc gttccatgga aagactaatc atctctgtca tcttggtgct cagtagaggg
    40201 aaagtattaa tcacttccaa ggttttcaca gctaagctct cagctttatc atagtagcca
    40261 ttaacagagt aatgcttgat agcagttctt aagcaggcaa tatagtagtc agcagtacgg
    40321 attgaatggt attcagtctg tttttttaga gtatctaata agtggtagag cctataatct
    40381 aaaaattcga ttgaaaagtg aggcaggata gatgaaaaga tattgacctc aattttgttc
    40441 cagtcgtcta attcactcag atagtttttg atatattcta gttcttggta atcttttgga
    40501 gtcagaaaag aatcgccgat gatgtttgcg atagcaaatt tattgactac acgattggca
    40561 atcacatagt gttcagcgat cccaggattt tccatgggtt gattaatcaa actaatgatc
    40621 tttttaaatt cacgcttata ggccaaatca gcaacttgag cctgtaattt atgaaattcc
    40681 gttggaaaca tctgtgtttt gagactatca aaggtctctt tatcaattcc aatattagaa
    40741 agaatctctt caaatttttg aatggttatt cgagatttcc cttgctcaaa ctgacgtaaa
    40801 aaatgaggag tcactgtagt cccagcagct tccttctgtg taaaccctct ttcctcacgg
    40861 atggtcttat agactaatcc ataaatactt tcatcttgca tcctaaatct cctcgaatcc
    40921 ttttttctat tctatcataa ttctaccagt tttt
//
LOCUS       DAVMMR010000013        39724 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMMR010000013 DAVMMR010000000
VERSION     DAVMMR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.013"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            9..89
                     /locus_tag="VYS74_000656"
     tRNA            9..89
                     /locus_tag="VYS74_000656"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VYS74_000657"
     tRNA            94..164
                     /locus_tag="VYS74_000657"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VYS74_000658"
     tRNA            179..251
                     /locus_tag="VYS74_000658"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="VYS74_000659"
     tRNA            265..336
                     /locus_tag="VYS74_000659"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            346..429
                     /locus_tag="VYS74_000660"
     tRNA            346..429
                     /locus_tag="VYS74_000660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Leu,seq:caa)
     gene            536..1813
                     /locus_tag="VYS74_000661"
     CDS             536..1813
                     /locus_tag="VYS74_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV3788729.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1889..3373
                     /locus_tag="VYS74_000662"
     CDS             1889..3373
                     /locus_tag="VYS74_000662"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV3788730.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3434..4714
                     /locus_tag="VYS74_000663"
     CDS             3434..4714
                     /locus_tag="VYS74_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3788731.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4718..5338
                     /locus_tag="VYS74_000664"
     CDS             4718..5338
                     /locus_tag="VYS74_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3788732.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5504..6616
                     /locus_tag="VYS74_000665"
     CDS             5504..6616
                     /locus_tag="VYS74_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3788733.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6939..7364
                     /locus_tag="VYS74_000666"
     CDS             6939..7364
                     /locus_tag="VYS74_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV3788734.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7375..7734
                     /locus_tag="VYS74_000667"
     CDS             7375..7734
                     /locus_tag="VYS74_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV3788735.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7841..8485
                     /gene="pcp"
                     /locus_tag="VYS74_000668"
     CDS             7841..8485
                     /gene="pcp"
                     /locus_tag="VYS74_000668"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3788736.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8513..9370)
                     /locus_tag="VYS74_000669"
     CDS             complement(8513..9370)
                     /locus_tag="VYS74_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV3788737.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9500..10642
                     /gene="tgt"
                     /locus_tag="VYS74_000670"
     CDS             9500..10642
                     /gene="tgt"
                     /locus_tag="VYS74_000670"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV3788738.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10740..12557
                     /locus_tag="VYS74_000671"
     CDS             10740..12557
                     /locus_tag="VYS74_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3788739.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12710..13861
                     /gene="nagA"
                     /locus_tag="VYS74_000672"
     CDS             12710..13861
                     /gene="nagA"
                     /locus_tag="VYS74_000672"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV3788740.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14024..15082
                     /locus_tag="VYS74_000673"
     CDS             14024..15082
                     /locus_tag="VYS74_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV3788741.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15233..15598
                     /locus_tag="VYS74_000674"
     CDS             15233..15598
                     /locus_tag="VYS74_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV3788742.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15673..16614
                     /locus_tag="VYS74_000675"
     CDS             15673..16614
                     /locus_tag="VYS74_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV3788743.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16562..17578
                     /locus_tag="VYS74_000676"
     CDS             16562..17578
                     /locus_tag="VYS74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV3788744.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRAGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17580..17906
                     /gene="comGC"
                     /locus_tag="VYS74_000677"
     CDS             17580..17906
                     /gene="comGC"
                     /locus_tag="VYS74_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV3788745.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVA
                     NRKVND"
     gene            17899..18303
                     /locus_tag="VYS74_000678"
     CDS             17899..18303
                     /locus_tag="VYS74_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV3788746.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18308..18568
                     /locus_tag="VYS74_000679"
     CDS             18308..18568
                     /locus_tag="VYS74_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788747.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18531..18992
                     /locus_tag="VYS74_000680"
     CDS             18531..18992
                     /locus_tag="VYS74_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3788748.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18970..19383
                     /locus_tag="VYS74_000681"
     CDS             18970..19383
                     /locus_tag="VYS74_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3788749.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            19520..20113
                     /locus_tag="VYS74_000682"
                     /pseudo
     CDS             19520..20113
                     /locus_tag="VYS74_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20174..21127
                     /locus_tag="VYS74_000683"
     CDS             20174..21127
                     /locus_tag="VYS74_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3788750.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGETEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21178..22368
                     /locus_tag="VYS74_000684"
     CDS             21178..22368
                     /locus_tag="VYS74_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV3788751.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22517..22888
                     /gene="rnpA"
                     /locus_tag="VYS74_000685"
     CDS             22517..22888
                     /gene="rnpA"
                     /locus_tag="VYS74_000685"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV3788752.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22863..23687
                     /locus_tag="VYS74_000686"
     CDS             22863..23687
                     /locus_tag="VYS74_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3788753.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23706..24692
                     /locus_tag="VYS74_000687"
     CDS             23706..24692
                     /locus_tag="VYS74_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV3788754.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24743..25366
                     /locus_tag="VYS74_000688"
     CDS             24743..25366
                     /locus_tag="VYS74_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV3788755.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQCLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25507..26964
                     /locus_tag="VYS74_000689"
     CDS             25507..26964
                     /locus_tag="VYS74_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3788756.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDREKYYNGEVQEEA"
     gene            26987..27268
                     /locus_tag="VYS74_000690"
     CDS             26987..27268
                     /locus_tag="VYS74_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3788757.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27344..27829
                     /locus_tag="VYS74_000691"
     CDS             27344..27829
                     /locus_tag="VYS74_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3788758.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27846..28511
                     /locus_tag="VYS74_000692"
     CDS             27846..28511
                     /locus_tag="VYS74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV3788759.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28515..29378
                     /locus_tag="VYS74_000693"
     CDS             28515..29378
                     /locus_tag="VYS74_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV3788760.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29380..30084
                     /locus_tag="VYS74_000694"
     CDS             29380..30084
                     /locus_tag="VYS74_000694"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV3788761.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30261..31934
                     /locus_tag="VYS74_000695"
     CDS             30261..31934
                     /locus_tag="VYS74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3788762.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32046..33137
                     /gene="ulaG"
                     /locus_tag="VYS74_000696"
     CDS             32046..33137
                     /gene="ulaG"
                     /locus_tag="VYS74_000696"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV3788763.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33251..35227
                     /gene="tkt"
                     /locus_tag="VYS74_000697"
     CDS             33251..35227
                     /gene="tkt"
                     /locus_tag="VYS74_000697"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3788764.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35344..35643
                     /gene="yajC"
                     /locus_tag="VYS74_000698"
     CDS             35344..35643
                     /gene="yajC"
                     /locus_tag="VYS74_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3788765.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35689..36117
                     /locus_tag="VYS74_000699"
     CDS             35689..36117
                     /locus_tag="VYS74_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV3788766.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36119..36529
                     /locus_tag="VYS74_000700"
     CDS             36119..36529
                     /locus_tag="VYS74_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788767.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36829..39480
                     /gene="adhE"
                     /locus_tag="VYS74_000701"
                     /gene_synonym="adhC"
     CDS             36829..39480
                     /gene="adhE"
                     /locus_tag="VYS74_000701"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV3788768.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12084 a         7101 c         8937 g        11602 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa
      361 ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg
      421 ccgccggtat agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt
      481 ttggtataat tatagttatt caaattttat ttagattaag aaagtgtagg ggagtatgtc
      541 ttgttctatc gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt
      601 gtttgtcgga attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt
      661 tgaagttatg aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa
      721 ggtagatgat tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt
      781 atttgaagtt tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga
      841 ggtcgaaaat cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa
      901 tcatgctatt gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt
      961 ggcttatcca cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa
     1021 atcagattta catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct
     1081 ggatatttca aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc
     1141 taaggcttat ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc
     1201 aagggatatt ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa
     1261 tgctagactc cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt
     1321 tactgcggaa cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt
     1381 ggctacgtcc gaaatccaag catttgctct gaatggggat gaggttatta tttaccccca
     1441 agagaaggat tttgaaactc atcgtagtta ccagtaccaa gatttaacga ctcgaggaac
     1501 agttgagttt cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt
     1561 tcacttggga ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt
     1621 ctttaaagta tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct
     1681 tacagatgag gaagaaacta cgattattga attttccaaa gacttactcc tactagctga
     1741 ggagggacta gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga
     1801 attgagccta taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga
     1861 cgagactata gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc
     1921 caacaataca gtaactgcca gccaagcaat tttgcaaggt ttggcgacgg acggcggttt
     1981 gtttacaccg gatacttatc caaaggtaga tttgaacttt gacaaattga aagatgcttc
     2041 ttaccaggaa gttgctaagc tagttttgtc agcattttta gatgacttta cagttgagga
     2101 gttggactac tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcacc
     2161 attagtgaaa ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt
     2221 taaggatatg gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg
     2281 tttggagaac aagattgtta tcttgacagc gacatctggt gacacgggga aagctgctat
     2341 ggcggggttt gcgaatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt
     2401 cagcaagatt caagagttac aaatgaccac tcagactggc gacaatactc atgttattgc
     2461 tattgatggt aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgacgtggc
     2521 tcttcgtgaa aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat
     2581 tggtcgtctg gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac
     2641 tggtgaaatt gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa
     2701 tatcttggct gccttttatg ccaaacaaat tggtttgcca gttggtaaat taatctgtgc
     2761 ttcaaatgac aacaatgttt tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg
     2821 tgagtttaag gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg
     2881 cttgattttc catcttttgg gaaataatgc tgaaaagaca actgaactta tgaatgcctt
     2941 gaacacgcaa ggacaatata agttgacaga ctttgatgca gagattttgg acctctttgc
     3001 agctgaatat gcgactgagg aagaaacggc agcagagatc aagcgtgttt gtgagttaga
     3061 ttcttatatc gaggaccctc atacagctgt tgcttcagca gtttataaaa aataccaatc
     3121 ggccactgga gatgtaacta agacagtgat tgcttcaaca gctagtccat acaagttccc
     3181 agtagttgca gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc
     3241 tcaattacat gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgaaatagc
     3301 tccaattcgt cacaagacaa cagtggcagc tgctgacatg caagcagcgg ttgaggctta
     3361 tttaggactt taagacagag ggagcaaact cggttgggaa accaactgag tttcttttca
     3421 tcaggaggag agattgttta agaaaaataa agacattctt aatattgcat tgccagctat
     3481 gggtgaaaac tttttgcaga tgctaatggg aatggtggac agttatttgg ttgctcattt
     3541 aggattgata gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc
     3601 gattttcatc gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa
     3661 agaccagtcg aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag
     3721 tttcctttta ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac
     3781 ggagagggat gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt
     3841 tctcttaggt ttaatgacta gtctaggagc cttgattcgt gcaacgcata atccacgtct
     3901 gcctctctat gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat
     3961 ttttgttctg gatatgggga tagctggtgt tgcttggggg acaattgtgt ctcgtttggt
     4021 tggtcttgtg attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt
     4081 agataaggaa ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc
     4141 tggagatgta gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa
     4201 tgcaatcgga gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc
     4261 aacggtcatg ctgttggccc gagcagttgg agaggatgat tggaaaagag ttgctagttt
     4321 gagtaaacaa accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata
     4381 tgtcttgggt gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag
     4441 tgttctagtg acactgtttt cactacttgg gacccctatg acgacaggaa cagtcatcta
     4501 tacggcagtc tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg
     4561 aatgtggtgt atccgcattg ggacaggata tctgatgggg attgtgcttg gttggggctt
     4621 gcctggtatt tgggcagggt ctctcttgga taatggtttt cgctggttat ttctacgcta
     4681 tcgttaccag cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg
     4741 ggatttagac gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac
     4801 ttttgctcag ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta
     4861 ttcggtgcaa gatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct
     4921 aaatcaggtg cgtgcccaga gtctggctga gaagaatgct caggtagttt tgatgccagg
     4981 tgcgcgtgag gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca
     5041 taaggggaac aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacaga
     5101 gattttaacc agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct
     5161 gctagataag tatcagttga attctgataa tacttattat ataggggatc ggactctgga
     5221 tgtggaattt gcccagaata gtgggattca aagcatcaac tttttagagt ctacttatga
     5281 agggaatcac aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata
     5341 aaaagattgt gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg
     5401 ttacaaggaa tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt
     5461 gtgttatgat agacaggtac tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5521 attagcgtca acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5581 tctttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa
     5641 aacaagttat accgtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     5701 agatgtaaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     5761 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     5821 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     5881 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagt
     5941 tacaaagaca gtgattgctt ctgaagaagt ggcaccatct acgggcactt ctgtcccaga
     6001 ggagcaaacg accgaaacaa ctcgcccagt tgaagaagca actcctcagg aaacgactcc
     6061 agctgagaag caggaaacac aagcaagccc tcaagctgca tcagcagtgg aagtaactac
     6121 aacaagttca gaagcaaaag aagtagcatc atcaaatgga gctacagcag cagtttctac
     6181 ttatcaacca gaagagacga aaataatttc aacaacttac gaggctccag ctgcgcccga
     6241 ttatgctgga cttgcagtag caaaatctga aaatgcaggt cttcaaccac aaacagctgc
     6301 ctttaaagaa gaaattgcta acttgtttgg cattacatcc tttagtggtt atcgtccagg
     6361 agacagtgga gatcacggaa aaggtttggc tatcgacttt atggtaccag aacgttcaga
     6421 attaggggat aagattgcgg aatatgctat tcaaaatatg gccagccgtg gcattagtta
     6481 catcatctgg aaacaacgtt tctatgctcc attcgatagc aaatatgggc cagctaacac
     6541 ttggaaccca atgccagacc gtggtagtgt gacagaaaat cactatgatc acgttcacgt
     6601 ttcaatgaat ggataaaccc gacttgataa catcattttg acgaatgaga tctagctttc
     6661 gtgatggaaa gcgattctcg ttcgtttttt ctttgtcata ctcttcgaaa atctcttcaa
     6721 accacgtcag ttttatctga aacttcaaag ctgtgctttg agcaacctgc gactagcttc
     6781 ctagtttgct ttttgatttt cattgagtat caatttgaat ggaaaatgga aagttatcat
     6841 cttgtaatga gttaagcaac attcttgcaa tctattttac tttatatcac aattaattag
     6901 tcaaatattg ataaatcaat aaaaagagag gggaagaaat gctagagatt caagatttac
     6961 tgtatcaact ccgcttgtct gagcaagcga gtacgcaatt gtttgaaaaa aggcttggga
     7021 ttagtttgac acggtatcag attttactgt ttttgctgga gcattctcct tgtaaccaaa
     7081 tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat ttcaaaattt
     7141 tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg gaagtgttgg
     7201 tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg caacatatca
     7261 gggttaagga agagatagaa agtatcttaa cagagtttga gagaacagaa ctcagccgtt
     7321 tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa atagatgtca
     7381 attattttaa caacgatcgt tgctttggag catttttaca ttttttattt ggaaagtatt
     7441 gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga attggctcat
     7501 ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggctctgct aggagtcttt
     7561 ctcttgtatg tcatttattt ctcacagaat ttagaaattg tgactatttt tgtcttattt
     7621 gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat tttgaaacaa
     7681 ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac ttgaaggtcg
     7741 attctaatct cgctaatcct ttttaatcca gaataaggga aatatgttat acttgttttt
     7801 aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat tagtgacagg
     7861 ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta aaggtttacc
     7921 agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttt ttcacaaatc
     7981 tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc tttgtattgg
     8041 gcaagctggt ggaagaacta gtttgacacc tgaacgagtg accattaatc aagacgatgc
     8101 atgcatttct gataacgaag ataatcaacc gattgaccgt cccattcgcc cagatggtgc
     8161 ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa aaaaagaggg
     8221 cttaccggcc tctgtttcca atacggcagg gacttttgtc tgcagccatt tgatgtatca
     8281 ggctctctat ttggtagaaa agaaatctcc atatgttaag gcaggtttta tgcatattcc
     8341 ttatatgatg gaacaggtgg tgaacagacc gactactcca gctatgagtt tagtggatat
     8401 tcggcgaggg atagaagcag caatcggcgc tataatagaa catggagatc aggaactcaa
     8461 gttggtaggc ggagaaactc attgatagaa aaaagcttga ggggaaaaac cttcaagctt
     8521 ttggacgttt tcgggccaat actgctcggt aaaacataat tttagtgcat tggatataag
     8581 gtaggagtga aaaactagca atgccaaagg taatccaatt gaggaagtac caaggaagaa
     8641 gctgtaaatc taggacaaag tgctggaact tgtagccctt cataaaggaa cggctagttt
     8701 ttaggattcg tcttggtggg acctgtccta ggtctagact ataacagaga agaaattcca
     8761 cctgtgaata ggcataatac tgtggaatat agaggatatt tcctacaatg atcaagatga
     8821 gacttgcaag aaagtagagt ccaaagacca tgaggaaacg ctcggtttca actgatgaga
     8881 gatctagatt tggaaactca ggatgtaggg tgacgaattt tttggctaaa aagctactat
     8941 aaaagaggag gtaaatccca agtaaattag ggatactcca taaaaagaga tagaaacgtt
     9001 tgagaagtag ggtcaaaaag gtttgagaaa agcgctcctc atcaaagaga gctaggctgt
     9061 tttttacaga tggctccgtt ttagaatctt tcatgagtgt cagtgttgca tagacggaac
     9121 tggtcaaaag aatagtcccg ataaaggaga ctagtagagg aaagaggtag gtttgaagta
     9181 tttggccaag tatgctgaaa aatggctgtt ctaaaacagt cccgtggatc cgagataagg
     9241 gattaagaaa accagataag atgaccagca tactgggaag gatatagagg agaaagagac
     9301 ggggggtgtc agcctgaaaa tgttttgact cctgacgaat tgtttttaaa tcaatttttg
     9361 gatagttcat tctcttatta taccatagtt cttatacata gttcgtgaca gttcctactt
     9421 tttttgataa aatcatacag tgtgtccttg ggcacactgt atgaactggg actgtctttc
     9481 ccagcttcgg aggtaaaaaa tgtcagattc accaatcaaa tatcgtttga ttaagaaaga
     9541 aaaacacaca ggagctcgtc tgggagaaat catcactccc cacggtacct ttccgacacc
     9601 tatgtttatg ccagttggga cacaagccac tgtcaaaact cagtcacctg aagaattgaa
     9661 ggagatgggt tcgggaatta tcctatcaaa cacctatcat ctctggcttc gccctggaga
     9721 tgaactcatt gcacgcgctg gtggtctcca caagttcatg aattgggacc agcctatctt
     9781 gacagatagt ggtggttttc aggtttattc tttagcagat agccgtaata tcacagaaga
     9841 aggagtaacc tttaaaaatc atctaaatgg ttctaagatg ttcctatccc cagaaaaagc
     9901 catctctatt cagaataatc tgggttcaga catcatgatg tcctttgatg aatgtcctca
     9961 gttttatcaa ccttatgact acgttaagaa atcgatcgag cgtaccagcc gttgggctga
    10021 gcgtggtttg aaggctcacc gtcgtccaca tgaccaaggt ttgtttggaa ttgtgcaagg
    10081 tgcaggattt gaagaccttc gccgccaatc agctcatgat cttgtcagca tggatttctc
    10141 aggctactct atcggtggtt tggcagtggg agaaacccat gaagagatga atgcggtctt
    10201 ggactttaca actcaactgc tacctgaaaa taaacctcgt tatctgatgg gtgtgggagc
    10261 gccagatagc ttgatcgatg gggtcattcg tggggtggat atgtttgact gtgtcttacc
    10321 gactcgaatt gctcgtaacg ggacttgtat gaccagtcaa ggacgtttgg ttgtgaaaaa
    10381 tgcccagttt gctgaggact ttacgccact ggatcctgag tgtgactgct acacatgtaa
    10441 taactataca cgcgcttacc ttcgtcacct gctcaaggct gatgaaacct ttggtatccg
    10501 cttgactagc taccacaatc tttacttctt gcttaacctg atgaagcaag tgcgacaagc
    10561 catcatggat gacaatctct tggaattccg tgagtatttt gtggaaaaat atggctataa
    10621 taagtcagga cgtaatttct aaaatggaat tgatataaaa aaatcctaag ttttctctta
    10681 ggatttttct tctttttttg atagaataaa gtgtacaatg aaaggaagaa taaactcgta
    10741 tgcgcattaa atggttttcc ttgattagga ttataggttt acttttggta ctcttgtatc
    10801 acttctttca gacgatcttt cctggaggat ttttcggggt agatgtcttt ttcacatttt
    10861 caggtttcct gattacagct ctactcattg aagaattttc taagaacaat gagattgatt
    10921 tgataggatt ttttagaaga cgcttttatc ggattgtgcc acctgtggtt ttgatggtct
    10981 tggtgaccat gccttttact ttcttggttc gccaagacta tgttgctgga attggtggcc
    11041 agattgcggg cgtcttaggc tttatgacca acttctatga actcctaaca ggtgggagtt
    11101 atgaatctca gttcattcct catttgtttg ttcataattg gagtttggca gttgaggttc
    11161 actactatat tctttgggga ttggcagttt ggttcttatc caaacaagct aaatcaaatg
    11221 gtcagttgaa ggggatggtc tttctcttat ctgctgctgc cttcttgatc agtttcttct
    11281 ccatgtttat tggtagtttt ctagtgacct cttattcctc tgtttatttc tccagtttaa
    11341 ctcatgtcta tccattcttt ttgggaagta tgttagcaac tattgtaggt gttcgtcaga
    11401 cgacttccct cgtcaagcag ttggataaaa tctgggattt acgaaagact ttggtagttt
    11461 ttggaggagg ctttggtttc ctagttcttt tgactttctt tgtcaaattc acttatcttt
    11521 ttgcctatct tatcggcttc ttgcttgcca gtcttgcagc tcttgccatg attctggcgg
    11581 cgcgtgtctt acatgaaaag acacatcata tacaggagcc gaagattatc agctttttag
    11641 cggatactag ctatgcggtt tatcttttcc attggccttt ctatatcatt ttctcacagt
    11701 tgacatcaaa tcttcttgct gtattactga ctctgatttg ttcttatggc tttgccagtc
    11761 tgtcattcta tgtattggaa ccttggattg caggcaagaa cacacctatt ttacaaactc
    11821 ttcgtcccct gccttatatt cacgcaattc ttgtagcagg tacaggaatc ttgaccatca
    11881 ttgtctgcac ggtgaccttg ttggcaccac aagtgggagc gtttgagaca gacttgactg
    11941 tcaatggctt gaagcaagct gcaacaaata ttggccagac caaggtgatg gcagaacggg
    12001 cagatgcaaa cagtttggga attgctgatg gcactatgtt aattggtgac tcagtggctt
    12061 taagggcaaa tacagcacta cagacagctc ttcctggagc acagattaac gcgcaggtca
    12121 gcgtaacaac caagaccgca aatgaaatca tgctaaataa tagccagaat aaatttttac
    12181 ctaagacggt ggtcattgcg actggggtaa ataatcctga gaattacaag gatgactggg
    12241 acagtatcgt gaaaaatctt cctaagggac accatatgat tttggtgact ccttatgagg
    12301 gagataagac aaaagagacc tatgccatcg ttgagaaggc tgctgcctat atgagagaat
    12361 tggcagagaa gacaccttac attacgatag cagattggaa tcaagttgcg aaagagcatc
    12421 cagaaatttg ggctggaaca gaccaggttc atttcgggag tgagagtagc actatcgaag
    12481 caggagcaaa attgtatgca gatacgattg ccacagcttt gcagacagct caagacaagc
    12541 cggttaaatc aaaataactt gtattaaaaa agagaagagt tggagaaatc cagctctttt
    12601 aaaatatctc caaaaaatag gtctatacca tttacaaata aaaaagaaag gtttataatg
    12661 taattgacat aataaattgt agaatcaatc ttttaaggag gttaacatta tgcctaacta
    12721 tattaaagcg gatcagtttt tctacccaca cggagttcgt cgaggtggtt acttggaact
    12781 tgtggacggc aagtttggga aacatgtaga gcagattcct gaaggggctg aggtgattga
    12841 ctatacaggt tatagcattg ccccaggtct tgtggatact catattcatg gatatgcagg
    12901 tgtagatgtg atggacaaca acattgaagg tacattgcat actatgagtg aaggacttct
    12961 tagtaccggt gttaccagtt tcttacccac aactttaaca gccacttatg agcaattgct
    13021 tgcagtcact gaaaatcttg gaaaccatta taaagaagca acaggtgcta agattcgtgg
    13081 gatttattat gaaggtccat atttcacaga aacttttaag ggggcacaaa atccaactta
    13141 tatgagagac ccgggtgttg aggagtttca ttcttggcaa aaagcggcaa atggcttgct
    13201 taataaaatt gcccttgcac cagaacgtga tggggtggaa gactttgttc gtacagttac
    13261 gggcgaaggt gtgacggttg ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa
    13321 agcagtcgat gctggagcga gtgtttgggt gcatgcctac aatggaatgc gtgggttgac
    13381 tcaccgtgaa ttgggtatgg ttggagccat gtaccaattg ccacatacct atgcagagtt
    13441 gatctgtgat ggtcaccacg tagatccaaa ggcttgcgaa attcttatca aacaaaaagg
    13501 aacagaaaac atcgctctta tcacagactg tatgacagct gggggattgg aagacggaga
    13561 ttatatgttg ggagaattcc cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac
    13621 aggtaacttg gcaggttcta tcctcaaact caaagatggt ttgaagaatg tggtcgaatg
    13681 gggaattgcg aatccgcatg aagcagtcat gatggccagc ttcaacccag ctaaatccgt
    13741 tcacatcgat gacgtctgtg gccaaatccg tgaaggctac gacgctgact tcatcgtatt
    13801 agataaagat ttggaattgg tagcaaccta tctagatggc gtaaaacgtt atcaagcata
    13861 agagagaagg cagaagttag agactagctt ctgctttttt atacagggta ctttactggt
    13921 aaataaaaag ttaaaaaaat cacaaaaaaa gcttgaagaa acaaagaaaa aggcttacaa
    13981 ttgaactata aatattacaa ataaaaaaac ggaggagtgc tttatgaaag cctatactta
    14041 tgttaaacca ggacttgctt cttttgttga tgtagacaaa ccagttattc gcaagccaac
    14101 agacgctatt gtgcgtattg taaaaaccac tatttgtgga acagacctcc atattatcaa
    14161 aggggatgtt cctacttgcc aaagtggtac cattcttggc cacgaaggga ttgggattgt
    14221 tgaagaagtt ggggaaggag tttccaactt caaaaaaggc gacaaggtct tgatttcttg
    14281 cgtctgtgcc tgtggtaaat gctactactg taaaaaagga atttatgccc actgtgaaga
    14341 cgaagggggc tggattttcg gtcacttgat tgatggtatg caggctgaat atctacgtgt
    14401 ccctcatgca gataatactc tttaccatac tccagaagac ttgtcagatg aagctttggt
    14461 tatgctgtca gacattctgc ctactggata tgaaattggt gtcttaaaag ggaaagtaga
    14521 acctggttgc agcgtagcca ttattggttc aggtccagtt ggattggctg ctcttttaac
    14581 agcccaattc tattcaccag ctaaattgat tatggtagac ctagacgata accgcttgga
    14641 aactgcccta tcattcggtg cgactcataa ggttaattct tcagacccta aaaaagccat
    14701 taaagaaatt tatgatttga cagatggtcg tggtgtggat gtcgctatcg aagctgtagg
    14761 gattcctgaa acatttgatt tctgtcaaaa gattatcggt gtagacggaa cggttgccaa
    14821 ctgtggtgtg catggtaaac cagttgaatt cgatttagat aaactttgga ttcgcaacat
    14881 caatgtaaca actggtttgg tatctacaaa tacgactcca caattgttga aagcacttga
    14941 aagtcataag attgaaccgg aaaaattggt aactcactat ttcaaactca gtgaaattga
    15001 aaaagcctac gaagtcttca gtaaggcagc agaccaccat gccattaagg tcattatcga
    15061 aaacgatatc tcagaagcct aagtagtaaa aatatttttg tacataagta aatagaaatt
    15121 cagtcatcca tcagatggtt ggatttttta tcaaaaaatt aagaaatgag catatttctt
    15181 tccttgtctg gtggaattgg ttataatata cggtacaaag gaatgaatga atatgtatcg
    15241 tgttatagaa atgtacggag attttgaacc gtggtggttc ttagaaggtt gggaagaaga
    15301 tattgtagca agtagaaaat ttgaccagta ttatgatgct ctcaaatact acaaaacttg
    15361 ctggtttaga ttggaacaag aatcgcctct ttataaaagt agaagcgact tgatgaccat
    15421 tttttgggac ccggaagacc aacgctggtg tgatgaatgt gatgagtatt tacaacaata
    15481 ccattctttg gctcttttgc aggatgagca ggttatccca gacgaaaaac tacgctcagg
    15541 ctatgaaaaa caaaccagtc aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag
    15601 aaaagtaact ttttggagtt gcttttttta tttttctaac gctttgcgaa tagtataggt
    15661 gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcggc tcggaaaaaa
    15721 ggggcgcaag acatttattt tgtccccaag ttagatgcct atgagcttca tatgagggta
    15781 ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc cgttatcagt
    15841 cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc
    15901 tgtgattatg cctatgacca gaagatggcg tctctacgtt tatctactgt aggcgattat
    15961 cgggggcatg agagtttggt tatccgtttg ttgcacgatg aggagcagga cttgcatttt
    16021 tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact ctatcttttt
    16081 gctggtccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa gtcactcttt
    16141 aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga cgacatgctt
    16201 cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa actttccttg
    16261 cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac ggcgcgtgca
    16321 gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc caagagtatc
    16381 cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt
    16441 ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga ctttgcaagc
    16501 agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca gcttcttaaa
    16561 gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa
    16621 aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg tggagaccat
    16681 ctccttttta gataggagtg ctttgttgga caagcagtgt gtgacccaga tgcgtgcggg
    16741 cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt caagtgctat
    16801 tgtcactcag ttatccctag ctgaagtcca tggcaatctc cacctgagtt tgggaaagat
    16861 agaagagtat ctagacaatc tggccaaggt caagaaaaaa ttaattgaag tagcgaccta
    16921 tcctttgatt ttgctgtttt ttcttctctt aattatgctg gggctacgga attacctgct
    16981 cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc cacaaatttt
    17041 tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt tttataaaag
    17101 aagttctaaa atgagtgtct tttctatctt agcacgcctt ccctttattg gaatctttgt
    17161 gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt cacagggaat
    17221 ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct ttaaagaaat
    17281 cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac
    17341 ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag ttaagtccaa
    17401 gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt ttacccgagt
    17461 caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac tgattatcgt
    17521 tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa atttttaaaa
    17581 tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga
    17641 tgttggtggt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct aatctgacca
    17701 agcaaaaaga ggcagtcaat gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc
    17761 aggcagaact ttatagcttg gaaaagaatg aagatgctag cctaagcaag ttacaagcag
    17821 atgggcgaat cacggaagaa caggctaaag cttataaaga ataccatgat aaaaatggag
    17881 tagcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct cttggctttg
    17941 agtcttgtga gtatccttgc cttgggcttg tccggctctg ttcagtccac ttttgcggca
    18001 gtagaggaac agattttctt tatggagttt gaagaactct atcgggaaac ccaaaaacgc
    18061 agtgtagcca gtcagcaaaa gactagtctg aacttagatg ggcagacgct tagcaatggc
    18121 agtcaaaagt tgccagtccc taaaggaatt caggccccat caggtcaaag tattacattt
    18181 gaccgtgctg ggggcaattc gtccctggct aaggttgaat ttcagaccag taaaggagcg
    18241 attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga aacaaaaaat
    18301 tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca gcattgcgac
    18361 cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga
    18421 agaagtcttg agggtagcta agatggccct gcagacgggg caaaatcagg taagcatcaa
    18481 cggagttgag attcaggtat tttctagtga aaaaggattg gaggtctacc atggttcaga
    18541 acagttgttg gcaatcaaag agccataagg tcaaggcttt taccttgtta gaatccctgc
    18601 ttgccctcat tgtcatcagt gggggattac tcctttttca agctatgagt cagctcctca
    18661 tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg tttgtggacc
    18721 aacttgaggt agaattagac cgttcgcagt tcgaaaaagt ggaaggcaat cgtttctata
    18781 tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa
    18841 ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca gctcagatta
    18901 cagaggacaa tcaactggtt cattttcgtt tccagtttca aaaaggctta gaaagggagt
    18961 tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta tgcagtcacc
    19021 atggcagcta tctttagtct tttgttgcaa ttttacttga accgacaagt cgcccactat
    19081 caagactatg ctttgaataa agaaaaattg gttgcttttg ccatggctaa acgaaccaag
    19141 gataaagccg agcaagaaag tggggaacag gtctttaatc taggtcaggt aagctatcaa
    19201 aacaagaaaa caagcttggt gacgactgtt cgtacggata agagtcaata tgagtttctg
    19261 tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt agcgactgat
    19321 tcaagcgaaa aagcggagaa gaaaaaatca gaagagaagc ctgaaaagaa agagaattcc
    19381 tagtcaattc aactataatg cgttgaatcc aaaatagtcc actgtagttt ctagaaaatt
    19441 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
    19501 tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgattc
    19561 ccctaaaaaa tcttcctcgc aaacttggta tgtcaagcag ccgagaaaca gattgatctt
    19621 ctatcagaca aagaaatttt agatttcggt ggtggcacgg atctattagc cttgccccta
    19681 gcccctagcc aagcaggcta agtcagtcac tcttgtagac atttctgaga aaatgttgga
    19741 gcaagctcgt ttgaaagtgg agcagcaagc aatcaagaat atccagtttt tggagcaaga
    19801 tttaccgaaa aatcccttgg agaaagagtt tgattgcctt gctgttagtc gggttcttca
    19861 tcatatgcct gatttggatg cggctctctc actgtttcat caacatttga aggaagatgg
    19921 gaaactcatc attgctgatt ttaccaagac agaagctaat catcatggat ttgatttagc
    19981 tgaactggaa aacaagctaa ttgagcatgg tttttcatct gtgcatagtc agattctcta
    20041 tagcgctgaa gacctgtttc aaggaaatca ctcagaattc tttttaacag tagcccaaaa
    20101 atcactcgcc tagtcaggga gtgatttttc tataaggatg gaaaaaagaa gggaaatttg
    20161 gtaagatagt aatatggatt ttgaaaaaat tgaacaagcc tatacctatt tactagagaa
    20221 tgtccaagtc atccaaagtg atttggcgac caacttttat gacgccttgg tggagcaaaa
    20281 cagcatctat ctggatggtg aaactgagct aaaccaggtc aaagacaaca atcaggccct
    20341 taagcgttta gcactacgca aagaagaatg gctcaagacc taccagtttc tcttgatgaa
    20401 ggctggggaa acagaaccct tgcaggccaa tcaccagttt acaccagatg ccattgcttt
    20461 gcttttggtg tttattgtgg aagagttgtt taaagaggag gaaattacta tcctcgaaat
    20521 gggttctggg atgggaattc taggcgctac tttcttgacc tcgcttgata aaaaggtgga
    20581 ttacttggga atggaagtgg atgatttact gattgatctg gcagctagca tggcagatgt
    20641 aattggtttg caggctggct ttgtccaagg agatgccgtt cgtccacaaa tgctcaaaga
    20701 aagcgatgtg gtcatcagtg acttgcctgt cggctattat cctgatgatg ccgttgcgtc
    20761 gcgtcatcaa gttgcttcta gccaagaaca tacttacgcc catcacttgc tcatggaaca
    20821 agggtttaag tacctcaagt cagatggata tgctattttt ctagctccga gtgatttgtt
    20881 gaccagtcct caaagtgatt tgttgaaagt ctggttgaaa gaggaggcaa gtctggttgc
    20941 tatgattagt ttacctgaaa atctctttgc taatgccaaa caatctaaga ctatttttat
    21001 cttacagaag aaaaatgaaa tagcagtaga gccttttgtt tatccacttg ctagcttgca
    21061 agatgcaagt gttttaatga aatttaaaga aaattttcaa aaatggactc aaggtactga
    21121 aatataaaat agattttgtt ataatagttg aaaacgctta aaaaggggta tcatgttatg
    21181 acaaaaacaa ttgcaatcaa tgcaggaagt tcaagtttga aatggcaatt atacttaatg
    21241 ccagaggaaa aagtgttagc caaaggtttg attgaacgta tcggtttgaa agattcaatt
    21301 tcaactgtaa aatttgacgg ccgttctgaa caacaaattt tggatatcga aaatcataca
    21361 caagccgtta aaattttatt ggatgacttg attcgtttcg atattatcaa ggcttatgac
    21421 gagattacag gtgttggaca tcgtgttgtt gctggtggag aatatttcaa agaatcaaca
    21481 gttgttgagg gagatgtttt agaaaaagtt gaagagttga gtttgttggc tcctctacac
    21541 aacccggcca atgcagcagg tgttcgtgcc ttcaaggaat tgttgccaga cattaccagt
    21601 gtagttgttt ttgatacttc cttccacaca agtatgccag agaaagctta tcgctaccct
    21661 ctaccaacaa aatattacac agaaaacaag gttcgtaaat acggtgctca tggtacaagt
    21721 caccagtttg tagcaggaga agctgcaaaa ctcttgggac gtccattaga agacttgaag
    21781 ttaattacct gtcatattgg taacggaggc tcaattacag ctgtgaaagc cggcaaatct
    21841 gtagacactt ctatggggtt cactcctctt ggtggtatta tgatgggaac gcgtacaggg
    21901 gatattgatc cagctatcat tccttattta atgcaatata cagaggattt taacacacca
    21961 gaagatatca gtcgtgttct taaccgtgaa tcaggtcttt tgggagtttc tgctaattct
    22021 agcgatatgc gcgatataga agcagctgta gcagaaggga atcacgaggc tagcttggct
    22081 tatgaaatgt atgttgaccg tatccaaaaa catatcggtc agtaccttgc agtgctaaat
    22141 ggagcagatg ccattgtttt cacagcaggt gtcggtgaaa atgcagagag tttccgtcgt
    22201 gatgtaatct cagggatttc gtggtttggt tgtgatgttg atgatgaaaa gaatgtcttt
    22261 ggcgttacag gagacatctc aacagaggca gctaaaatcc gtgtcttggt tattccaaca
    22321 gatgaagaat tagtcattgc ccgtgacgtt gaacgcttga aaaaataagt gaaactaaaa
    22381 aaatattcaa tacaaggagt tgggaaagtt atttttccag cttctttttc tgatgaaatt
    22441 gtccaaaacc ttgctatgat tggctttttt gaaaaatatg gtataatagt agtaatttaa
    22501 tagatggagt tgagttttga agaaaaactt tcgtgtaaaa agagagaaag attttaaggc
    22561 gattttcaag gaggggacaa gttttgctaa tcgcaaattt gtggtctacc aattagaaaa
    22621 ccagaaaaac cattttcgag taggtctatc agttagcaaa aaactgggga atgccgtcac
    22681 tagaaatcaa attaagcgac ggattcggca tattatccag aatgcaaaag ggagtctggt
    22741 agaagatgtc gactttgttg tcattgctcg aaaaggagtc gaaaccttgg gatacgcaga
    22801 gatggagaaa aatctactcc atgtattaaa attatcaaag atttaccggg aaggaaatgg
    22861 gagtgaaaaa gaaactaaag ttgactagtt tgctaggact gtctctgtta atcatgacag
    22921 cctgtgcgac taatggggta actagcgata ttacagccga atcggctgat ttttggagta
    22981 aattggttta cttctttgcg gaaatcattc gctttttatc gtttgatatt agtatcggag
    23041 tggggattat tctctttacg gtcttgattc gtacagtcct cttgccagtc tttcaggtgc
    23101 aaatggtggc ttctaggaaa atgcaggaag ctcagccacg cattaaggcg cttcgagaac
    23161 aatatccagg tcgagatatg gaaagcagaa ccaaactaga gcaggaaatg cgtaaagtat
    23221 ttaaagaaat gggtgtcaga cagtcagact ctctttggcc gattttgatt cagatgccgg
    23281 ttattttggc cctgttccaa gccctatcaa gagttgactt tttaaagaca ggtcatttct
    23341 tatggattaa ccttggtagt gtggatacaa cccttgttct tccgatttta gcagcagtat
    23401 tcaccttttt aagtacttgg ttgtccaaca aagctttgtc tgagcgaaat ggcgctacga
    23461 ctgcgatgat gtatgggatt ccagtcttga tttttatctt tgcagtttat gcgccaggtg
    23521 gagtcgccct atactggaca gtgtctaatg cttatcaagt cttgcaaacc tatttcttga
    23581 ataatccatt caagattatc gcagagcgcg aggccgtagt acaggcacaa aaagatttgg
    23641 aaaatagaaa aagaaaagcc aagaaaaagg ctcagaaaac gaaataaata aggaggaatc
    23701 tggtaatggt agtatttaca ggttcaactg ttgaagaagc aatccagaaa ggattgaaag
    23761 aattagatat tccaagaatg aaggctcata tcaaagtcat ttctagggag aaaaaaggct
    23821 ttcttggtct atttggtaaa aaaccagccc aagtggatat tgaagcgatt agtgaaacga
    23881 ctgttgtcaa agcaaatcaa caggtagtaa aaggcgttcc gaaaaaaatc aatgatttga
    23941 acgagcctgt gaagacggtt agtgaagaaa ccgttgacct tggtcatgtg gttaatgcta
    24001 ttaaaaaaat agaggaagaa ggtcaaggta tttctgatga agtcaaggct gaaatcttaa
    24061 aacatgaaag acatgccagc actatcttag aagaaactgg tcacattgag attttaaatg
    24121 aacttcaaat cgaggaagcg atgagggaag aagcaggcgc tgatgacctt gaaactgagc
    24181 aagatcaaac tgaaaatcaa gacttgaaag agatgggctt gaaggtcgag caaagttatg
    24241 atattgccca ggtggctacg gatgtgactg cctatgttca agcgattgtg gatgacatgg
    24301 atgttgaagc tacactttca aatgattata accgtcgtag catcaatcta caaattgaca
    24361 ccaacgaacc aggtcgtatt atcggctacc atggtaaagt cttgaaggcc ttgcaactgt
    24421 tggctcaaaa ttatctttac aaccgctatt ccaaaacctt ctacgttaca atcaatgtca
    24481 atgattatgt cgaacaccgt gcagaagtct tgcagaccta tgcgcaaaaa ttggcgaatc
    24541 gtgttttgga agaaggtcgc agtcataaaa cagatccaat gtcaaatagc gaacgcaaga
    24601 ttatccatcg tattatttca cgtatggatg gcgtgactag ttactctgaa ggtgatgagc
    24661 caaatcgcta tgttgttgta gatacagaat aagtaaaatc agggttgacc tgattttttg
    24721 ctagttagag gaggttaaac tgatgttgaa taagataaga gactatctag acttttctgg
    24781 tttgcagtac cgtagtcctg ataaagcggg agcagagcga gagaagatgc tggcgttccg
    24841 ccacaaagga caagaggccc gaaaggcttt tacagaactg gccaaagcct ttcaagcaag
    24901 ccatccagaa tggcaactcc aacagactag tcagtggatg aatcaggccc agtgtttgag
    24961 accacatttt tgggtttatc tacagagaga cggacaagtg acagaaccta tgatggcctt
    25021 acgtttgtat gggacatcta ctgactttgg aatttctttg gaagtcagtt tcatcgaacg
    25081 taagaaggat gagcaaacac tgggcaagca gaccaaagtt ttagacattc caaccgttaa
    25141 agggatttat tatctaacct actctaatgg tcaaagtcaa cggtgggagg cgaatgaaga
    25201 aaagcgtcgt actttacgcg agaaggtgag aagtcaagaa gttcgaaaag ttttagtgaa
    25261 ggtagatgtt cctatgacag aaaattcgtc tgaagaagaa atcgtagaag gcttattgaa
    25321 gtcttattct aaaattcttc cctattatct agctacgaga aaataagata atttgtaaaa
    25381 catcataaat catcataaat catacagtcc aagagtgaac agtccgctgt gtaattcttg
    25441 gtctttttgt ttgcgctttc gcattatata ataaacttac aaaaacaatt caaaaggaga
    25501 acaattatgg aagtcgtttc aagtgttcta aattggtttt ctagcaatat tttgcagaat
    25561 cccgcatttt tcgtaggttt attggtgttg ataggatatg cacttttgaa aaaacctgcc
    25621 catgacgttt tttcagggtt tgttaaagca acagtagggt atatgttgct taacgtgggt
    25681 gctggtggtt tggttacaac ctttcgtcca atcttagcag ctcttaacta caaattccaa
    25741 attggtgcag cggttatcga cccttacttt ggacttgctg cagcaaacaa caaaattgca
    25801 gcagagtttc cagattttgt tggaactgca actacagctc tattgattgg ttttggaata
    25861 aatatcttgc tcgtagctct tcgaaagatt acgaaggtaa gaaccctctt tattactggt
    25921 cacatcatgg tacaacaagc tgcaacagta tctcttatgg ttctattctt agtaccacaa
    25981 ttgcgcaatg cttacggtac agcagcgatt ggtatcatct gtggacttta ctgggcagtt
    26041 agttcaaata tgactgttga ggcaactcaa cgcttgactg gtggtggcgg atttgcgatt
    26101 ggtcaccaac agcaatttgc aatctggttt gtagataaag tagcaggacg ctttggtaag
    26161 aaagaagaaa gtttagacaa tcttaaatta cctaagttcc tctcaatctt ccacgataca
    26221 gttgttgcat ctgctacctt gatgctcgta ttcttcggag ccattctttt aatcttgggt
    26281 ccagacatta tgtctaataa agaagtcatc acttcaggaa ctctattcaa tcctgctaaa
    26341 caagatttct ttatgtacat tatccaaaca gcctttacct tctcagttta cttgttcgtt
    26401 ttgatgcaag gtgtccgaat gttcgtatct gagttgacaa acgccttcca aggtatttca
    26461 aacaaattgt tgccaggttc attcccagcg gttgacgttg cagcttctta tggatttggt
    26521 tctccaaatg ctgtcttgtc aggatttacc tttggtttga ttggtcaatt gattacaatt
    26581 gtcttgctca tcgtctttaa aaatccgatt cttattatta caggatttgt accagtgttc
    26641 tttgacaatg cagccattgc ggtctacgct gataaacgcg gcggatggaa agcggctgtt
    26701 atcctttcct ttatatcagg tgtccttcaa gttgctctag gagctctttg tgtggccctt
    26761 ctcgatttgg catcttatgg tggctaccat ggaaatatcg actttgaatt cccatggctt
    26821 ggatttggat atatcttcaa ataccttggt attgttggtt atgtacttgt gtgtctcttc
    26881 ttgcttgtta ttcctcaact tcaatttgcc aaagcaaaag atagagagaa atattacaac
    26941 ggtgaagttc aagaagaagc ttagtatcta gaaaaggaga aataaaatgg ttaaagtatt
    27001 agcagcgtgc ggaaatggaa tgggttcatc aatggttatc aagatgaagg ttgaaaatgc
    27061 tctccgtaag cttaatcaaa cagattttac agtcaattca tgcagtgtcg gtgaagctaa
    27121 aggtttagca gtaggatatg acatcgtaat cgcttctctt catttgattc aagaattgga
    27181 agggcgaact aatgggaagt taattgggct tgataacttg atggatgata aagaaatcac
    27241 cgaaaaactc agtcaagcac tacagtaaaa ggttggaggg gctggacaga aactgagagt
    27301 tatcgtttct gtccttctcc ctctttaaat aaaggaggca gatatgaatt taaaacaagc
    27361 tttaattgac aatgactcga tccgactagg tttagaggct aacaattgga aagaagcagt
    27421 caaggtagca gtagatccct taattgaaag tggggcaatt ttgccagagt attacgatgc
    27481 tatcattgaa tcgactgaag agtatgggcc ttactatatc ttgatgccag gtatggctat
    27541 gccccacgct agacctgaag ctggtgtgca aagtgatgcc ttttcattga ttaccttaca
    27601 aaatcctgtt gtattttcag atgggaaaga ggtatctgtt ttgttggcac tagcagcaac
    27661 aagttcaaaa attcacacaa gtgtagccat tccacaaatt attgccctgt ttgaattaga
    27721 agattctatt gcacgtttac aggcttgcca gactaaagaa gatgtcttgg ctatgattga
    27781 agaatctaag gatagccctt atctcgaagg attggatttg gaaagttaga aagaggaata
    27841 aagaaatgac aaaaagaata cctaatttac aagttgcatt agaccattca gacttgcaag
    27901 gagcgattaa agcagctgtt tctgttggtc aggaagtaga tattatcgaa gctggaactg
    27961 tttgcttgct tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt ttcccagata
    28021 agattattgt ggcagacaca aaatgtgctg atgctggtgg aacagttgct aaaaataatg
    28081 cggttcgtgg agcagactgg atgacttgta tctgttgtgc aaccatccct actatggaag
    28141 cagctctaaa ggctatcaag actgaacgag gagaacgagg cgaaatccag atcgagcttt
    28201 atggcgattg gacttttgaa caagctcagc tttggctaga tgcaggtatt tcacaagcta
    28261 tttatcacca atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa aaagacctta
    28321 ataaggttaa aaaactcatt gacatgggct tccgtgtatc tgtaacaggt ggtctagatg
    28381 tagatactct caaactcttt gaaggtgttg atgtctttac ctttatcgca ggtcgtggaa
    28441 ttacagaggc tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa atcaaacgaa
    28501 tttgggggta aatcatggta cgtccaattg gaatttatga aaaggcaacc ccaacacact
    28561 gtacttggct agaacgttta aattttgcca aggagttagg ctttgatttt gtcgagatgt
    28621 ctattgacga acgtgacgag cgtttagcaa gacttgactg gagtaaggaa gaacgcttgg
    28681 aagttgtcaa agcaatctat gaaactggtg ttcgtattcc ttctatctgt ttttcaggcc
    28741 atcgtcgcta cccattgggt tcaaaagatc cagttctaga ggaaaaatct ctagaactca
    28801 tgaaaaaatg tatcgaatta gctcaagact tgggagttcg tacgattcaa ttagctggtt
    28861 acgatgttta ctatgaggaa aagtcacccc agacacgcca acgttttatc aaaaatttga
    28921 gaaaagcctg tgactgggct gaagaagctc aggtggtact tgctattgaa attatggatg
    28981 atcctttcat caatagcatc gaaaaatatt tggctataga aaaagagatt gactctccct
    29041 tcctctttgt atatccagat attggtaatg tgtctgcatg gcataatgat atctatagtg
    29101 agttttatct tggtcatcat gccatcgcag ctctccatct caaggatact tatgcagtga
    29161 cagaaagttc aaagggccag ttccgagatg taccttttgg gcaaggttgt gtcaaatggg
    29221 aagaagcttt cgatatttta aaggaaacca attataatgg acctttccta atcgaaatgt
    29281 ggtctgaaaa ttgtgaaaca gtagaagaaa cacgcgcagc cattcaagag gcgcaagctt
    29341 ttctctatcc actcattaag aaagcaggtt tgatgtaaga tgaatcaagt aatcaatgct
    29401 atgcgtaaac gagtttgtga tgccaatcaa tcattgccaa aacatggact tgtcaaattt
    29461 acctggggga atgtatctga agtcaatcgc gaactcggtg tcattgttat caaaccatca
    29521 ggcgtggatt atgacgaatt gacacctgaa aacatggtag tgactgatct agatggtaag
    29581 atcctagaag gggatttaag accatcttcc gacctcccaa ctcatgtgca attatataag
    29641 gcttggtcag aaattggtag tgtggttcac acccattcga cagaagctgt tggttgggct
    29701 caggcaggtc gtgatattcc tttctacgga acaacccatg cagattattt ctacggttca
    29761 atcccttgcg cccgtagttt gaccaaggac gaagtagaag tggcctatga aaaagatact
    29821 ggcctggtta tcgtagaaga atttgaacat cgcggactta acccggttga agtaccagga
    29881 attgttgtac gcaatcacgg tccattcacc tggggcaaaa atccagagaa tgctgtttat
    29941 cactctgtcg tactagagga agtatcaaag atgaatcgct ttacagaaca aatcaatcca
    30001 agagttggac ctgctcccca gtacatacta gaaaaacact accaacgtaa acatggacca
    30061 aatgcttatt atggtcaaaa gtaagaacga tgaaggagga gaaaaagata aatttagctc
    30121 ctctttttac atttgatttt tattgagagt aaagttggag ttgaagtaat tttgaaagat
    30181 tttttagaaa tagcgcttga tatatatggt aaaataaaaa gaattgctgt gatatcaata
    30241 gatttggggg attttttaat atggtactgg ataaggcaag ttgtgatttg cttcaatatt
    30301 tgatggatca agaaacgtcc aaaacgatta tggcgatttc gaaagatttg aaagagtcaa
    30361 gaaggaaaat ttattatcac attgacaaaa tcaatgctgc tctggttgac gaggcgcttc
    30421 acatcattag tattccacga attggtattc acttaacgga agagcagaga gatgcttgtt
    30481 gtaaactatt atcggaagta gattcgtacg attatatcat gagtgcgcat gaacgtatga
    30541 tgataatgtt actatggata ggtatttcta aagaacgtat tacgattgaa aaattgatag
    30601 agttaacaga ggtatctagg aatactgttc tcaatgattt gaatagtatt cgttatcaac
    30661 taactttgga acaatatcag gtgatcttgc aagtgagcaa gtcacaggga taccaccttc
    30721 atgcccaccc tcttaataaa attcagtatc ttcaatcgct tctatatcat atttttatgg
    30781 aagaaaatgc cacttttgta tctattttag aagataagat gaaagagagg ttagatgatg
    30841 agtgtttgct ttctgttgaa atgaaccaat tttttaagga acaggttcct ttagttgaac
    30901 aagatttagg gaagaaaata aaccatcatg aaataacttt tatgttgcag gttctacctt
    30961 atttgctgtt aagctgtcat aatgttgaac agtatcaaga aagacatcag gatatagaga
    31021 aagaattttc tttgataaga aaaagaatag agtatcaggt gtctaagaaa ttaggagaac
    31081 ggttgtttca aaagtttgaa atttctttgt caggacttga agtttctctt gtagctgttc
    31141 tcctcctctc ctatcgtaag gatttggata ttcatgcaga aagtgatgat tttcggcaat
    31201 taaaacttgc tttagaagaa tttatctggt attttgaatc acaaatccga atggagattg
    31261 agaacaagga tgatttgtta cgaaatttga tgatccactg taaagccttg ttatttagaa
    31321 agacttacgg tattttttct aaaaatcctc taacaaaaca aattcgatcc aagtatggag
    31381 aattattttt agtcactaga aaatctgcgg aaattttaga aggagcatgg tttattcggc
    31441 taacagacga tgatattgcc tatttgacga ttcatattgg aggattttta aagtatacac
    31501 catcgtctca aaaaaatatg aaaaaagttt atctcgtttg tgatgaaggt gttgcggttt
    31561 cgagactttt gctgaaacaa tgcaaacttt attttccaaa tgagcaaatt gacactgtat
    31621 ttacaacaga acaatttaag agtgtggaag atattgcaca agttgatgta gtgattacta
    31681 ctaatgatga tttggatagc agatttccga ttttaagggt taatcctatc cttgaagcag
    31741 aagatatttt gaaaatgcta gactatctta aacacaatat atttcgtaat aagagcaaaa
    31801 gtttcagtga aaatctttct agtcttattt cgtcttatat tgtagacagc aagttggcta
    31861 gtaagttcca agaagaggtt caaacactta taaatcaaga aatagtagtt caagcttttt
    31921 tggaagatat ttgaaggaca gtccaatgat gaacacaaac ctgtgttttt cttggtcttt
    31981 tttagtgttt tgaagggtgg tatactaatc tcaaagataa caattatatc caaaggaggc
    32041 aacatatgcc aaacgtcaaa gaaattacaa gagagtcatg gattttagcc actttcccag
    32101 agtggggaac atggttgaac gaagaaatcg aagaagaagt cgtacctgaa ggcaattttg
    32161 ccatgtggtg gctaggcaac tgtggtactt ggattaagac accagctggt gctaacgttg
    32221 tcatggacct ttggtcaaac cgtggaaaat caaccaaaaa agtgaaagat atggttcgtg
    32281 ggcaccaaat ggcaaatatg gcaggtgttc gtaagttgca accaaacttg cgtgttcagc
    32341 caatggttat cgatccattt gctatcaacg aactagacta ttacttagtt tcacacttcc
    32401 acagtgatca tatcgaccca tacacagctg cagcaattct caacaattct aagttagagc
    32461 atgttaagtt tatcggtcct taccactgtg gacgaatctg ggaaggatgg ggtgttccaa
    32521 aagaacgtat catcgttgtt aaaccaggtg acactatcga attaaaagat atgaagattc
    32581 atgcagtaga atcatttgac cgtacttgct tggtaactct cccagtgaac ggtgctgatg
    32641 agacaggcgg tgaacttgct ggcttggctg ttacagatga agaaatggct caaaaggctg
    32701 ttaactatat ctttgaaaca ccaggtggaa ccatctatca tggtgcagat tctcacttct
    32761 caaactattt tgcaaaacat ggtaaagact ttaaaattga tgttgctttg aataactatg
    32821 gtgaaaatcc ggtaggtatc caagacaaaa tgacatctat cgatcttctt cgtatggcag
    32881 aaaatctgcg taccaaagtc attatcccag ttcactatga tatctggtct aacttcatgg
    32941 cttctactaa tgagattcta gaactttgga aaatgcgaaa agatcgcttg caatacgatt
    33001 tccatccatt tatctgggaa gttggcggta agtacactta tcctcaagat caacacttag
    33061 tagaatacca tcatccacgt ggttttgatg attgttttga acaagactct aacattcaat
    33121 ttaaagcttt gctataatat aaaaatattc tttggaggtt atccgtatgt tgcatgatac
    33181 ggataatttt catataatag taaaatagaa tgtgtgattc aataatcacc tcaaatagaa
    33241 aggaaattct atgtcaaatc tatctgttaa tgcaattcgt tttctaggta ttgacgccat
    33301 taataaagcc aactcaggtc atccaggtgt ggttatggga gcggctccga tggcttacag
    33361 cctctttaca aaacaacttc atatcaatcc agctcaacca aattggatta accgcgaccg
    33421 ctttattctt tcagcaggtc atggttcaat gctcctttat gctcttcttc acctttctgg
    33481 ttttgaagat gtcagcatgg atgagattaa gagtttccgt caatggggtt caaaaacacc
    33541 aggtcaccca gaatttggtc atacggcagg gattgatgct acgacaggtc ctctagggca
    33601 agggatttca actgctactg gttttgccca agcagaacgt ttcttggcag ccaaatataa
    33661 ccgtgaaggc tacaatatct ttgaccacta tacttacgtt atctgtggag acggagactt
    33721 gatggaaggt gtctcaagcg aggcagcttc atacgcaggt ttgcaaaaac ttgataagtt
    33781 ggttgttctt tatgattcaa atgatatcaa cttggatggt gagacaaagg attcctttac
    33841 agaaagtgtt cgtgaccgtt acaatgccta cggttggcat actgccttgg ttgaaaatgg
    33901 aacagacttg gaagccatcc atgctgctat cgaaacagca aaagcttcag gcaagccatc
    33961 tttaattgaa gtgaagacgg ttattggata tggttctcca aacaaacaag gaactaatgc
    34021 tgtacacggc gcccctcttg gagcagatga aactgcatca actcgccaag ccctcggttg
    34081 ggactacgaa ccatttgaaa ttccagaaca agtatatgct gatttcaaag aacatgttgc
    34141 agaccgtggc gcatcagctt atcaagcttg gactaaatta gttgcagatt ataaagaagc
    34201 tcatccagaa ctggctgcag aagtagaagc catcatcgac ggacgtgatc cagtcgaagt
    34261 gactccagca gacttcccag ctttagaaaa tggtttttct caagcaactc gtaattcaag
    34321 tcaagatgcc ttgaatgttg tggcagctaa attaccaacc ttcctaggtg gatcagctga
    34381 ccttgctcac tcaaacatga cttatatcaa gacggatgga cttcaagacg ataccaatcg
    34441 cttgaatcgt aacattcagt ttggtgttcg tgaatttgca atgggaacaa tcttgaacgg
    34501 gatggccctt catggtggac ttcgtgtata cggtggaact ttcttcgtct tctctgacta
    34561 tgtgaaggca gctgtccgct tgtcagcctt acaaggactt cctgtgactt atgtctttac
    34621 ccatgattca atcgcagttg gggaagatgg tccgactcac gaaccagttg agcatttagc
    34681 aggtcttcgt gctatgccaa atctaaatgt tttccgtcca gcagatgcgc gtgaaacgca
    34741 agcagcttgg taccttgcag tgacaagtga gaaaacacca actgcccttg tcttgacacg
    34801 tcaaaacttg actgttgaag atggaacaga cttcgacaag gttgctaaag gtgcttatgt
    34861 tgtatatgaa aatgcagccg actttgatac catcttgatt gcgacaggtt cagaggttaa
    34921 tcttgctgtc tcagctgcta aagaattggc tagtcaaggc gaaaaaatcc gcgtaatcag
    34981 catgccatct acagatgtct ttgataaaca aaatgcagct tacaaggaag agattcttcc
    35041 aaatgcagtc cgccgtcgtg ttgcagtcga aatgggtgca agtcaaaact ggtacaaata
    35101 tgttggtctc gatggtgccg ttctaggtat tgataccttc ggagcctctg ccccagcacc
    35161 aaaagtattg gcagaatatg gctttactgt cgaaaatctt gtaaaagttg ttcgaaattt
    35221 gaaataatcc taaaaatcag ggcgtaagct ctggtttttc ttaccagaaa agtaaggtac
    35281 aatcttgtaa aagtagctga aatttgatat agtagtccta tgtaaaagac aaaggagaaa
    35341 acaatgaatc caaatattac ttttttaatc atgcttgtag gtatgatggc cttgatgttc
    35401 tttatgcaac gttctcaaaa gaaacaagct caaaagcgta tggaaagctt aaataaacta
    35461 caaaaaggct atgaagtgat tacaatcggt ggactttatg gaacagtcga tgaagtagat
    35521 acggagaagg gaacaatagt tcttgacgta gatggagttt acttgacttt tgaactagct
    35581 gctatcaaga cagtattacc gctgaaagaa acagcttcac tagaaggcgc aattgaaaaa
    35641 taagacggga tcacgaactc ccgttttcta taaaagaaag gaaatgggat gaaaaaatta
    35701 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    35761 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    35821 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    35881 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    35941 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    36001 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    36061 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    36121 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    36181 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    36241 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    36301 tggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    36361 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    36421 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    36481 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    36541 caaaggaggt attattagaa tcacaaggta agcgcttacc tgtaaatccc tttctttcca
    36601 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    36661 caaagataaa acttgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    36721 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    36781 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    36841 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    36901 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    36961 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    37021 tttgaagaaa caggacgtgg tgtatttgaa gacaaagcaa ctaagaactt gtttgcttgt
    37081 gaacacgtag taaacaatat gcgccacact aagacagttg gcgttatcga agaagacgat
    37141 gtaacaggat tgactcttat tgctgaacca gttggtgttg tttgtggtat tactccaaca
    37201 acaaacccaa catcaacagc aatcttcaaa tcattgattt cattgaagac acgtaaccca
    37261 atcgtctttg ccttccatcc atcagcacaa gaatcatctg ctcatgcagc tcgtatcgtc
    37321 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tgcaatggat tactcaacca
    37381 tctatggaag caacaagtgc ccttatgaac cacgaaggtg ttgcgacaat ccttgcaaca
    37441 ggtggtaatg ccatggttaa ggcggcttat tcatgtggta aaccagctct tggggtaggt
    37501 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    37561 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    37621 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    37681 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    37741 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    37801 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    37861 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    37921 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    37981 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    38041 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    38101 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    38161 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    38221 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    38281 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    38341 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    38401 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    38461 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    38521 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    38581 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    38641 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    38701 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    38761 actgtggcaa tcgtagaccc tgctttggta ttgacagttc caggatttgt tgctgctgat
    38821 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    38881 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    38941 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    39001 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    39061 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    39121 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    39181 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    39241 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    39301 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attatccttc
    39361 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    39421 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    39481 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    39541 tctatgataa aaggcatcct atcaaggttt ttgaatacct gataggatgc ctttttatga
    39601 tgttgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat atagcagatt
    39661 gaaactagaa tagtacatat ctgcttctaa aacattgtta gaattcgatt tgactgtcct
    39721 gatc
//
LOCUS       DAVMMR010000014        39168 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVMMR010000014 DAVMMR010000000
VERSION     DAVMMR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.014"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            718..1200
                     /locus_tag="VYS74_000702"
     CDS             718..1200
                     /locus_tag="VYS74_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEV3788769.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
     gene            1197..1793
                     /locus_tag="VYS74_000703"
     CDS             1197..1793
                     /locus_tag="VYS74_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3788770.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
     gene            1774..2265
                     /locus_tag="VYS74_000704"
     CDS             1774..2265
                     /locus_tag="VYS74_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788771.1"
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSE
                     LSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITL
                     SFSILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKNLRLTVVFSVPFLFNAIMSII
                     NSF"
     gene            2645..4798
                     /locus_tag="VYS74_000705"
     CDS             2645..4798
                     /locus_tag="VYS74_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV3788772.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            4811..6160
                     /locus_tag="VYS74_000706"
     CDS             4811..6160
                     /locus_tag="VYS74_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV3788773.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            6330..7037
                     /locus_tag="VYS74_000707"
     CDS             6330..7037
                     /locus_tag="VYS74_000707"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV3788774.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            7094..10819
                     /locus_tag="VYS74_000708"
     CDS             7094..10819
                     /locus_tag="VYS74_000708"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV3788775.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            10912..12354
                     /locus_tag="VYS74_000709"
     CDS             10912..12354
                     /locus_tag="VYS74_000709"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV3788776.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            12391..13413
                     /locus_tag="VYS74_000710"
     CDS             12391..13413
                     /locus_tag="VYS74_000710"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV3788777.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            13410..13955
                     /gene="purN"
                     /locus_tag="VYS74_000711"
     CDS             13410..13955
                     /gene="purN"
                     /locus_tag="VYS74_000711"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV3788778.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            14039..14548
                     /locus_tag="VYS74_000712"
     CDS             14039..14548
                     /locus_tag="VYS74_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3788779.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            14573..16120
                     /gene="purH"
                     /locus_tag="VYS74_000713"
     CDS             14573..16120
                     /gene="purH"
                     /locus_tag="VYS74_000713"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV3788780.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            16242..17504
                     /gene="purD"
                     /locus_tag="VYS74_000714"
     CDS             16242..17504
                     /gene="purD"
                     /locus_tag="VYS74_000714"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV3788781.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            17907..18395
                     /gene="purE"
                     /locus_tag="VYS74_000715"
     CDS             17907..18395
                     /gene="purE"
                     /locus_tag="VYS74_000715"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV3788782.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            18382..19455
                     /gene="purK"
                     /locus_tag="VYS74_000716"
     CDS             18382..19455
                     /gene="purK"
                     /locus_tag="VYS74_000716"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV3788783.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            19478..21865
                     /locus_tag="VYS74_000717"
     CDS             19478..21865
                     /locus_tag="VYS74_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3788784.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKRVKDYEKQISDLEDKKLNIDIAVDEINKSLNYIFFSKNRLAI
                     QNQNGKYYLLSRGKSVVPSRVSVGERNALALCYFFTEIIQQRELADAYSHEYFIVIDD
                     PISSFDMENKVGITSYLKYCLTRFFKGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCK
                     EEEGGQKSKYKKLELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMR
                     KILEAYGSFNYKKGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTL
                     SIDFFDTISDEERKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            22038..23336
                     /gene="purB"
                     /locus_tag="VYS74_000718"
     CDS             22038..23336
                     /gene="purB"
                     /locus_tag="VYS74_000718"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV3788785.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVFASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(23391..27329)
                     /gene="strH"
                     /locus_tag="VYS74_000719"
     CDS             complement(23391..27329)
                     /gene="strH"
                     /locus_tag="VYS74_000719"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3788786.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(27644..28360)
                     /locus_tag="VYS74_000720"
     CDS             complement(27644..28360)
                     /locus_tag="VYS74_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3788787.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            28708..30495
                     /locus_tag="VYS74_000721"
     CDS             28708..30495
                     /locus_tag="VYS74_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV3788788.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            30492..30968
                     /locus_tag="VYS74_000722"
     CDS             30492..30968
                     /locus_tag="VYS74_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3788789.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            30996..31901
                     /locus_tag="VYS74_000723"
     CDS             30996..31901
                     /locus_tag="VYS74_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3788790.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            31888..32703
                     /locus_tag="VYS74_000724"
     CDS             31888..32703
                     /locus_tag="VYS74_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3788791.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            32710..33114
                     /locus_tag="VYS74_000725"
     CDS             32710..33114
                     /locus_tag="VYS74_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3788792.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            33413..34579
                     /locus_tag="VYS74_000726"
     CDS             33413..34579
                     /locus_tag="VYS74_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV3788793.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            34695..35732
                     /locus_tag="VYS74_000727"
     CDS             34695..35732
                     /locus_tag="VYS74_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV3788794.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            35940..36794
                     /locus_tag="VYS74_000728"
     CDS             35940..36794
                     /locus_tag="VYS74_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3788795.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            36931..37188
                     /locus_tag="VYS74_000729"
     CDS             36931..37188
                     /locus_tag="VYS74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV3788796.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(37230..37592)
                     /locus_tag="VYS74_000730"
     CDS             complement(37230..37592)
                     /locus_tag="VYS74_000730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788797.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(37646..37942)
                     /locus_tag="VYS74_000731"
     CDS             complement(37646..37942)
                     /locus_tag="VYS74_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3788798.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(37932..38093)
                     /locus_tag="VYS74_000732"
     CDS             complement(37932..38093)
                     /locus_tag="VYS74_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV3788799.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            38163..38927
                     /locus_tag="VYS74_000733"
     CDS             38163..38927
                     /locus_tag="VYS74_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV3788800.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT        11153 a         7286 c         8880 g        11849 t
ORIGIN      
        1 aattataagt aaattgttct tatttttatc tataggacta ttgtctatag aacaaggaca
       61 agttgtaatt tatgtcatac tactcaatta ttttatttta gtcatgggga tattagataa
      121 tcatgcgagt aatttctatt ttaaaagcag tcgaatgagt caaaaaagat ttcgtagcag
      181 tctatttata ttgcttcgtt tttgttggtt aaataaaggg ttaacttttt cttttatttt
      241 gattagtatt cttctttcct atctattttg gtatatgatg acgatgttat caattgatga
      301 cacaaaaata ccaatattat ttctttatac ctcaatatat attacattaa tggaggcaat
      361 aatagggaat cataaatcct caattggggt agatctagct ttacttcaat tatctgctct
      421 taatcattct aaaatgaatt tcttttttaa gaatgcacaa ctaatgatgt tgacgcattt
      481 aatctctttc ttacaatttt cattgttgtt atgtttgatt ccggtgacaa gttatagtaa
      541 tgatataaaa agtattttgt tgaatttgtt attgattcca ttcaattatt ttatagccta
      601 tgtctattat aagaaagctc tatttttaat taaaggtaca gttagtttat tgagatggag
      661 ttttctggtt ttatattgtt ttctgataat atttgtaatt ttagtagggg gcattagatg
      721 agaaaaagtg gttttctgat ttactatgtg ttattttgta tagtctgttt tttactaggt
      781 ttacttttaa gctttatatt aaaaaatgag acttttgcta ctatacctcc gaaactggaa
      841 ttacaattta cattgacagt tttacaggca aattctacaa attttttgct atatctattc
      901 tgttttccga tttcgccctt attgcaactt tttgatttgc tatctttagg aatacaattg
      961 ggtatatctg gtcagatgat tggaatagta gctacaataa aaggaatatt acctcatgct
     1021 cttattgaaa taccgaattt tatttggtat caaggtatga gtcagtatct tttataccga
     1081 ttattgaagg ataggggaat taaaaatata cttatttata taaaaagtaa tttaaaatgg
     1141 tatatcattt cttatctttt attaatttta gcagcgttga tagagggaat taaattatga
     1201 aaattgaaaa tgaatctaaa agttatgata aatgtatatt gaaaaatata tcactagaga
     1261 ttgataaggg aatttacaga gttgaaggag agaatggttc aggaaagagc acccttctac
     1321 aacttttagc tgggctagag acttttgata gtggtttaaa gaattgtgtc tccaaagatg
     1381 tactatacct tgataccaat cagcttgggg ttgttccatt tactatagaa gaaaatttgc
     1441 agttgttgtg tgattctttt caaatccatc tatcatttga ggataaagaa caaattaata
     1501 ctttttttga taataaaatc ggagataact atatgacagc atctactgga acaaaattca
     1561 aattaggctt atccttaatt tttgctaaaa attgggatta tatttttatt gatgagactt
     1621 tatcaacagt ggatgcaaga agtattgata tgattgctaa acgcttaatt tccatgaaag
     1681 aaagctctac tattatatat gtatctcata atttattgaa tcaagaattg attagcaaat
     1741 ctcaaaggat tttaatagag gaaggacggg tttatgaaat ttcaaacaaa taatagttta
     1801 tacaaattaa tgaatctgat tatttatctt tttcttttat atttgtttat tgatttactg
     1861 atgaagtcgg gaattttaga tgaacaaatt gataaattat ctgaactatc taatcttttt
     1921 acaattaaaa tagaaacaat attgacagtg atttctgttg ttttcacatt tatagcaact
     1981 cttattgaat atggattttc tctctttctg attacattat tttttgataa gaataagagg
     2041 aactttggag atattctttt atctaaaaat ataaccttaa gttttagcat tttactttgt
     2101 tatttattta atccatcgga tataaagttt gcctatttca gcgtattgac agtattacta
     2161 attgggataa caaattatgc gagatttaaa aatttaagat tgacagtagt gttttctgta
     2221 ccatttttat ttaatgctat aatgtctatt ataaatagtt tttagaaata ctaaaatttt
     2281 tttctttact taataatgat atgaaatcat ttcggttttg ttaacttgta attcttatat
     2341 tttctaggta gaacgtttgt tctgcctatt tttttatcca aaaagtgcag ttgggaggga
     2401 gataggctca tttgggaagg aagtccagtt tttgtttagt gattggggta agatagttgt
     2461 tatcagatga gtttatactc ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc
     2521 gtatatatgt gactgacttc gtcagtccta tctacaacct caaaacagtg ttttgagcag
     2581 cctgcggcta gtttcctagt ttgctctttg attttcattg agtattaggg aaaaggagat
     2641 gaatatgaaa tttgggaaac gtcactatcg tccgcaagtg gatcagatgg actgcggtgt
     2701 agcttcatta gccatggttt ttggctacta tggtagttat tattttttgg ctcacttgcg
     2761 agaattggct aagacgacca tggatgggac gacggctttg ggcttggtca aggtggcaga
     2821 ggagattggt tttgagacgc gagccattaa ggcggatatg acgctttttg acttgccgga
     2881 tttgactttt ccttttgttg cccatgtgct taaggaaggg aaattgctcc actactatgt
     2941 ggtgactggg caggataagg atagcattca tattgccgat ccagatcccg gggtgaagtt
     3001 gactaaactg ccacgtgagc gttttgagga agaatggaca ggagtgactc tttttatggc
     3061 acctagtcca gactataagc ctcataagga acaaaaaaat ggtctgctct cttttatccc
     3121 tatattagtg aagcagcgtg gcttgattgc caatatcgtt ttggcaacac tcttggtaac
     3181 cgtgattaac attgtgggtt cttattatct gcagtctatc attgatacct atgtgccaga
     3241 tcagatgcgt tcgacactag ggattatttc tattgggcta gtcatcgtct acatcctcca
     3301 gcaaatcttg tcttacgctc aggagtatct cttgcttgtt ttggggcaac gcttgtcgat
     3361 tgacgtgatt ttgtcctata tcaagcatgt ttttcacctc cctatgtcct tctttgcgac
     3421 acgcaggaca ggggagatcg tgtctcgttt tacagatgct aacagtatca tcgatgcgct
     3481 ggcttcgacc atcctttcga ttttcctaga tgtgtcaacg gttgtcatta tttcccttgt
     3541 tctattttca caaaatacca atctcttttt catgacttta ttggcgcttc ctatctacac
     3601 agtgattatc tttgccttta tgaagccgtt tgaaaagatg aatcgggata ccatggaagc
     3661 caatgcggtt ctgtcttctt ctatcattga ggacatcaac ggtattgaga ctatcaagtc
     3721 cttgaccagt gaaagtcagc gttaccaaaa aattgacaag gaatttgtgg attatctgaa
     3781 gaaatccttt acctatagtc gagcagagag tcagcaaaag gctctgaaaa aggttgccca
     3841 tctcttgctt aatgtcggca ttctctggat gggggctgtt ctggtcatgg atggcaagat
     3901 gagtttgggg cagttgatta cctataatac cttgctggtt tactttacca atcctttgga
     3961 aaatatcatc aatctgcaaa ccaagcttca gacagcgcag gttgccaata accgtctaaa
     4021 tgaagtgtat ctagtagctt ctgagtttga ggagaagaaa acagttgagg atttgagctt
     4081 gatgaaggga gatatgacct tcaagcaggt tcattacaag tatggctatg gtcgagacgt
     4141 cttgtcggat atcaatttaa ccgttcccca agggtctaag gtggcttttg tggggatttc
     4201 agggtcaggt aagacgactt tggccaagat gatggttaat ttttacgacc caagtcaagg
     4261 ggagattagt ctgggtggtg tcaatctcaa tcagattgat aaaaaagccc tgcgccagta
     4321 catcaactat ctgcctcaac agccctatgt ctttaacgga acgattttgg agaatcttct
     4381 tttgggagcc aaggagggga cgacacagga agatatctta cgggcggtcg aattggcaga
     4441 gattcgagag gatatcgagc gcatgccact gaattatcag acagaattga cttcggatgg
     4501 ggcagggatt tcaggtggtc aacgtcagag aatcgctttg gcgcgtgctc tcttgacaga
     4561 tgcgccggtc ttgattttgg atgaggcgac tagcagtttg gatattttga cagagaagcg
     4621 gattgtcgat aatctcattg ctttggacaa gaccttgatt ttcattgctc accgcttgac
     4681 tattgctgag cggacagaga aggtggttgt cttggatcag ggcaagattg tcgaagaagg
     4741 aaagcatgct gatttgcttg cacagggagg cttttacgcc catttggtca atagctagaa
     4801 agaggagagg atgaaaccag aatttttaga aagtgcggag ttttataatc gtcgttacca
     4861 taatttttcc agtagtgtga ttgtacccat ggcccttctg ctcgtgtttt tacttggctt
     4921 tgcaactgtt gcagagaagg agatgagttt gtccactaga gctactgtcg aacccagtcg
     4981 tatccttgca aatatccagt caactagcaa caatcgtatt cttgtcaatc atttggaaga
     5041 aaataagctg gttaagaagg gggatctttt ggttcaatac caagaagggg cagagggtgt
     5101 ccaagcggag tcctatgcca gtcagttgga catgctaaag gatcaaaaaa agcaattgga
     5161 gtatctgcaa aagagcctgc aagaagggga gaaccacttt ccagaggagg ataagtttgg
     5221 ctaccaagcc acctttcgcg actacatcag tcaagcaggc agtcttaggg ctagtacatc
     5281 gcaacaaaat gagaccatcg cgtcccagaa tgcagcagct agccaaaccc aagccgaaat
     5341 cggcaacctc atcagtcaaa cagaggctaa aattcgcgat taccagacag ctaagtcagc
     5401 tattgaaaca ggtacttcct tggccggtca gaatctagcc tactctcttt accagtccta
     5461 caagtctcag ggcgaggaaa atccccaaac taaggttcag gcagttgcac aggttgaagc
     5521 acagatttct cagttagaat ctagtcttgc tacttaccgt gtccagtatg caggttcagg
     5581 tacccagcaa gcctatgcgt cagggttaag cagtcaattg gaatccctta aatcccaaca
     5641 cttggcaaag gttggtcagg aattgaccct tctagcccag aaaatcttgg aggcagagtc
     5701 aggtaagaag gtacagggaa atcttttaga caaggggaaa attacggcaa gtgaggatgg
     5761 ggtgcttcat cttaatcctg agaccagtga ttctagcatg gttgcagaag gtgccctact
     5821 agcccaactt tatccatctt tggaaagaga agggaaagcc aaactcacag cctatctaag
     5881 ttcaaaagat gtagcaagaa tcaaggtcgg tgattctgtt cgctatacta cgactcatga
     5941 tgccgggaat caactttttc tagattctac tattacaagt attgatgcga cagctactaa
     6001 gactgagaaa gggaatttct ttaaaatcga ggcggagact aatctaactt cggagcaggc
     6061 tgaaaaactt aggtacgggg tggaaggccg tctacagatg attacgggca agaaaagtta
     6121 tctacgttat tatttggatc aatttttgaa caaagagtaa tgttcgtgtt tttagagtta
     6181 aataattttt aaactgtgag aaagattctt cttgcagttt tttctttaca atttttgaaa
     6241 aacatctgct atttattcgg ttaaattctt gtgttttttg gttttttgtg gtaaaatgtg
     6301 ctcaagtaat acgaaaggcg aactttaaaa tgtcaaaaca attgatctat tcgggaaaag
     6361 ctaaagatat ctatacaact gaggatgaaa atcttattat ttcaacttac aaggaccagg
     6421 cgactgcttt caatggtgtc aagaaggagc agattgcagg taagggagtc ttgaataatc
     6481 agatttcatc ttttattttt gagaaattaa atgcggctgg tgtggcgact cactttgtgg
     6541 agaaactttc agacacggaa caactcaata aaaaggttaa gattattcct ttggaagtcg
     6601 tgctccgcaa ctatactgct ggttcctttt caaaacgttt tggtgtggat gagggaatcg
     6661 ccttggagac tccgattgtc gaattttact acaaaaatga tgatttggat gatccattta
     6721 tcaatgatga gcatgtgaaa ttcctacaga ttgcggatga ccagcagatt gcctacttga
     6781 aggaagaaac gcgtcgtatc aatgaactat tgaaagtctg gtttgctgag attgggctta
     6841 aattgattga ctttaagcta gagttcggtt ttgacaagga tggcaagatt atcttggcag
     6901 acgaattttc accagataac tgccgcttgt gggatgctga tggcaaccac atggataagg
     6961 atgttttccg tagaggattg ggagaactaa ccgacgttta tgagattgtt tgggaaaagt
     7021 tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag ctgaaataaa
     7081 ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt ttcaggtcaa
     7141 gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct tgaaaagtat
     7201 tcgtattgtg caagtatatg atgtatttga cttggttgag gacttgtttg cacctgcaga
     7261 gaagcacatt ttctctgagc aggtaaccga ccatgtttta gatgaagtat ctgtgcaggc
     7321 ggatcttgct aactatgctt tctttgccat tgaaagtcta ccagggcagt ttgaccagcg
     7381 tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg tgacagtcaa
     7441 cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg aagctgtcaa
     7501 aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgatag ggattgccaa
     7561 gcaggagttt tcagagtcag acaagaccat tccaaaattg cctttctttg aaagctatac
     7621 agcagaagac tttgctcgct acaaggccga acaagggatg gccatggaag tggatgattt
     7681 gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga ctgaactcaa
     7741 ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga cagagttgaa
     7801 acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct atgacaagta
     7861 tattgccatg cgcgaggaat taggtcggtc tgaaaaacca caaaccttga tggatatggc
     7921 gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg aagtctctga
     7981 cgaaatcaat gcctgctcag ttgaaattga agtggacgtt gatggtgtca aggaaccttg
     8041 gctcctcatg tttaaaaacg aaacccacaa ccatccaaca gaaattgagc catttggtgg
     8101 agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt cctatgttta
     8161 ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg aaactcgtgc
     8221 tgggaaattg ccacaacaag tcatttctaa aacagcaact catggttatt cttcatatgg
     8281 taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag gctttgtagc
     8341 taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg ttgtccgtga
     8401 aaaacctgaa gcaggtgatg tgatcatcct tctcggaggt aaaacaggtc gtgatggtgt
     8461 cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga ctgctggtgc
     8521 tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct tccgtaatgg
     8581 caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggtg tctgtgtggc
     8641 tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc ttaaatacca
     8701 gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg cggtcgtggt
     8761 tcgtcctgaa gatgtggata ccttcgttgc cgaatgtaac aaagaaaata ttgatgctgt
     8821 tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg gtgagacaat
     8881 cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg tcgatgccaa
     8941 agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg aaacactgga
     9001 atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag gattacagac
     9061 tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg gtcgttacca
     9121 actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg tgactcatac
     9181 tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc cataccacgg
     9241 tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg ccaactggtc
     9301 taaggctcgt ttctcttacc aagaatattt cgagcggatg gacaagcaag cagagcgttt
     9361 tggtcagcca gtagccgccc ttctaggctc tattgaagca caaattcagc ttggcttgcc
     9421 atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg ttccgccaac
     9481 cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc cagaatttaa
     9541 agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag agattgattt
     9601 tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc ataaagtgac
     9661 atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg ctacctttgg
     9721 aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga cagctcaatt
     9781 aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga ttggacaaac
     9841 gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca agcttgacag
     9901 tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag cgaaagaact
     9961 agaagaagta ccagctgtgg tatcagatgt tgtgattaaa gccaaagaaa aggttgaaaa
    10021 acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt cagctaaggc
    10081 cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga atgaagaagc
    10141 tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata ttctcttctt
    10201 tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta tcgtcaatat
    10261 cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg gtggcttgat
    10321 tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac catacggaaa
    10381 ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca accaacacgt
    10441 ggccaagatg gtggaaactc gtattgccaa taccaactca ccatggttgg ctggtgtgca
    10501 agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg tcgtgacggc
    10561 tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg ttgactttaa
    10621 cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg ccatcgaagg
    10681 aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac gttatgagga
    10741 tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat cagcggttaa
    10801 acatttcact ggaaaataag acttacagat tttctaatag atagtatcag taatgtaaaa
    10861 gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa aatgacatac
    10921 gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca tccagatgct
    10981 gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga gggggcagga
    11041 atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct tttatcagaa
    11101 gttttcagaa atccagccaa tttggataaa ttgacaggag ctggtgcgat tgggcatgtg
    11161 cgttatgcga ctgctggcga agcttctgtt gataacatcc agcccttcct cttccgtttt
    11221 cacgatatgc agtttggctt ggctcataat ggaaatctga ccaatgcagc ctctctcaag
    11281 aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga aatcttggct
    11341 cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga agcgctcagc
    11401 cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat tgcggctctt
    11461 gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc ggtggttgtt
    11521 tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga tttgaagcca
    11581 ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac agatgatact
    11641 cagttggcga tttgttctat ggagtatatc tactttgctc gccctgattc taatatccac
    11701 ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg agaatttaag
    11761 catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc tatgggattt
    11821 gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata cacccagcga
    11881 acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa actgtctgct
    11941 gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat tgtacgtgga
    12001 acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga ggttcacgtt
    12061 gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat ccagacccgt
    12121 caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat tggtgcggac
    12181 agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga aacagatgcg
    12241 ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc tctctatgac
    12301 tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa gtaggcgaca
    12361 gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata tgcccagtcg
    12421 ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca cgtggcccgt
    12481 acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt tgacctttcc
    12541 aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg aaccaagctc
    12601 atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt ggccatgtgt
    12661 gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta cgtagcgaca
    12721 gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga aggttgtgtg
    12781 caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat gtacggcgaa
    12841 gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca aatcattgac
    12901 ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg gattcactca
    12961 aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga agtcctacca
    13021 gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat ctatgtcaag
    13081 gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat cacaggtggt
    13141 ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga aattgatgaa
    13201 agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca gattaaacac
    13261 gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt cagccctgaa
    13321 aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg tcgcatcgtc
    13381 aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt gcctctggta
    13441 atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc ttttcagacc
    13501 atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc tatgcttttg
    13561 aactcaagga gtttgagagc aagacagact acgaagcagc ccttgtcgaa ctcttggaag
    13621 aacaccagat tgacttggtt tgcctagcag gctacatgaa aatcgttgga ccaaccttat
    13681 tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca gaatttccag
    13741 gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt gtgaccattc
    13801 actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt gtgccacgac
    13861 tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag tacaggttgt
    13921 atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca tatgatatct
    13981 ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg tatttgtaat
    14041 gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt tattaacttg
    14101 gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct tagctccaag
    14161 gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa ttgtttttgc
    14221 tgaaatgtta tttaatctga tttcctttat tccattaggt gtttgtttcc ctttgataaa
    14281 aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt tattgtttga
    14341 gtgcttacag tatattttag caataggtat aacagatata acggatttga ctttaaatac
    14401 gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt tcaaatcaca
    14461 gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt atcttgtttt
    14521 actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata taatgactaa
    14581 acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc aagaactcaa
    14641 aaaacttggt tgggagatta tctcaacagg tggaactaag gttgcccttg ataatgctgg
    14701 ggtggatacc attgctatcg atgatgtgac tggtttccca gaaatgatgg acggtcgtgt
    14761 gaagaccctc cacccaaata tccacggagg gcttctcgct cgtcgtgact tggatagcca
    14821 cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg tcaaccttta
    14881 cccatttaag gaaactatcc ttaaaccaga tgtgacttat gctgatgcag ttgaaaatat
    14941 cgatattggt gggccgtcta tgcttcgttc agcagcgaaa aatcatgcca gtgttacagt
    15001 tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa acggcgaaac
    15061 ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag cggcttatga
    15121 cgccttgatt gcagaatact tcacagctca agtgggtgaa agcaagcctg aaaaactcac
    15181 tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac aagacgcgga
    15241 cttttaccag aaagctttgc ctacagatta ctctattgct tcagccaaac agctcaacgg
    15301 gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta tcatccgtga
    15361 cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg gaattggtca
    15421 agctgatgac atcgagactg cttgggacta cgcttatgag tctgacccag tatctatctt
    15481 tggtgggatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga agatgcacgg
    15541 cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag ctattttgat
    15601 caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag aggctagcga
    15661 agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc aagacgtggt
    15721 caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaactg agacagaagc
    15781 gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg gtattatcgt
    15841 gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg tggcttctgt
    15901 tcgccttgcc attgaccaag ccaaagatcg tctgaacggg gcggtccttg cttcagatgc
    15961 cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta aggccatcat
    16021 ccagcccggt ggctctgtcc gtgaccaaga attcatcgaa gcagcggata aatacggctt
    16081 gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa gggaagaaaa
    16141 cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa cgaacttttt
    16201 tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg cttgttgtcg
    16261 gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa gacgtggaaa
    16321 aagtctttgt agctcctggg aatgacggta tgactctgga tggtctggaa ttggtaaata
    16381 tatctatttc cgaacattat aaattgattg acttcgcaaa ggccaacgat attgcttgga
    16441 cctttatcgg tccagatgac gcccttgcgg ctggtatcgt ggatgatttc catgcagctg
    16501 gtctcaaagc ctttggtccg acaagattgg cagcggagct ggagtggtcc aaggatttcg
    16561 ccaaggaaat catggtcaaa tatggtgttt cgacagcagc ctatggcaca ttttcagatt
    16621 tcgaggaagc aaaggcctat atcgaaaagc atggtgcgcc tatcgtagtc aaggcggatg
    16681 gcttggcgct tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg gtcgaagccg
    16741 ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg gttattgagg
    16801 aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat aagttctaca
    16861 tcatgccaac ggctcaggac cacaaacgtg cctacgatgg cgacaaaggg cctaacacgg
    16921 gtggtatggg tgcctatgcg ccagttccac acttaccaca gagtgtagtt gatacagcgg
    16981 ttgacaccat tgtcaagcca gttctagaag ggatgattaa agaaggtcgc ccttatctgg
    17041 gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatt gagttcaacg
    17101 ctcggttcgg agatccagaa actcagatta tcttgcctcg cttgacctct gactttgctc
    17161 aaaatatcac agatatcctg gatagcaagg agccaaatat catgtggacg gacaagggtg
    17221 tgactctggg tgtggttgtc gcatccaagg gctacccgct agactatgaa aggggcgttg
    17281 agttgccagc caagacagaa ggcgatgtca tcacctacta tgcaggggct aagtttgcgg
    17341 aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt accacagcag
    17401 ataccgtcaa agaagcccaa gccagcatct accaagaact atcccaacaa aaaatagaag
    17461 gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat aagaataacg
    17521 cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa cagtgaatgt
    17581 tctggtcagg gggaaacttg gagaccgtag gctcaaagtt taggaatgaa accgaaggtt
    17641 tgcttccgcc tccatcacct aagaccatta tcaaaaagaa aaataaaaat tcacaaaata
    17701 cgttaatgat cgtatggttt gcgagcgtta gcgagctaat atagaacaat caccgccgtt
    17761 gtgaaagaac gattggatga taatccaatc gttcagggaa attggaagac cttgggtttc
    17821 caatttaggc atgagacacc tttggtggct gctgccgtcc ctcacaagct aaggtgattg
    17881 ttgaaaaaga ggaaaaagga gaagaaatga aaccagtaat ttccatcatc atgggctcaa
    17941 aatccgactg ggcaaccatg caaaaaacag cagaagtcct agaccgcttc ggtgtagcct
    18001 acgaaaagaa agttgtttcc gcacaccgta caccagacct catgttcaaa catgcagaag
    18061 aagcccgtag tcgtggcatc aagatcatca tcgcaggtgc tggtggcgca gcgcatttgc
    18121 caggcatggt agctgccaaa acaacccttc cagtcattgg tgtgccagtc aagtctcgtg
    18181 ctcttagtgg agtggattca ctctattcta tcgttcagat gccgggtggg gtgcctgttg
    18241 cgaccatggc tatcggtgaa gctggagcga ctaacgcagc tctctttgcc ctccgtctcc
    18301 tctctgtaga agataagtcc attgcggatg cacttgccaa ctttgctgaa gaacaaggaa
    18361 aaatcgcaga ggagtcgtca aatgagctca tctaaaacaa tcggaattat cggtggcggt
    18421 caactgggtc agatgatggc catttctgct atctacatgg gccacaaggt tatcgcgctg
    18481 gatcctgcgg cggattgccc ggcctctcgc gtggcggaaa tcattgtggc accttataac
    18541 gatgtggacg ccctccgtca gttggcagac cgttgcgatg tcctcactta tgagtttgaa
    18601 aatgtcgacg ctgacggttt ggatgccgtt atcaaggatg gacaactccc tcaaggaaca
    18661 gatctgctcc gcatttcgca aaatcgtatt tttgaaaagg actttttgtc aaacaaggct
    18721 caagtcactg tggcacccta caaggtcgtg acttctagcc tagacttggc agatatcgac
    18781 ttgtcgaaaa actatgtcct caagactgcg actggtggct acgatggtca tggacaaaag
    18841 gttattcgtt cagaagcaga cttggaagca gcctatgcgc tagcagactc agcagactgc
    18901 gtcttggaag aatttgtcaa ctttgacctt gagatttctg tcatcgtgtc aggaaatggc
    18961 aaggaggtga cgtttttccc agttcaggaa aatatccacc gcaacaatat cctgtctaag
    19021 accatcgtac cagcccgcat ttctgaaagt ctagtagaca aggctaaagc tatggcagtg
    19081 cgaatcgcag aacaactcaa cttgtctgga actctctgtg tggaaatgtt tgcgactgat
    19141 gatgacgtta ttgtcaatga aatcgcccca cgaccacata actctgggca ctattctatt
    19201 gaagcctgtg atttctctca gtttgacacc catattctcg gtgttctcgg agcaccatta
    19261 ccagtcatca aactccatgc gcctgccgtc atgcttaacg ttctcggcca gcatgttgag
    19321 gccgctgaaa aatatgtcac agaaaattca agcgcccacc tccacatgta tggtaaaata
    19381 gaagcgaagc acaaccgcaa gatgggacat gtgactttga ttagtaatga accagatagt
    19441 gtgggagagt tttaggggag aggggtctaa agaaaaaatg tttgaaaaaa ttaaaggaat
    19501 taatataaaa tcaggtattt ttgaagatga aacaaaatta gaactctttg aaggaaattt
    19561 tgagggaaca aatccagtac aaaacgatag ggcaagttta ttgtttggaa ggaatggttc
    19621 agggaaaagt accattgctc gaggaataaa tcaattaaaa aatggtgaga ttggaacaga
    19681 tagagtatca tttattgata aaaataataa taatattgta cttagtgata ctgaacgaaa
    19741 atcgatattt gttttcaatg aacactatgt tgatcagaaa gtcaaaattg ctcaagaagg
    19801 gttagataca attgtaattt taggtgaaca ggttgatatc gatgaggaac tggatagatt
    19861 aagaacgcag ttatcagaat cacaaattga atctcaagat tattatgctg aatatgaaga
    19921 atatttagat gaaaaaaatg aaaaatctcc agatttttgg aaaaaggaaa tgacagattc
    19981 tttgaaagga gtaggcaact gggccgaaag agatagggag ataaaaggaa atcgagctgc
    20041 atctcccgtt cataataata cctttcaaaa ttttgttgat ttacaaccaa tcttagataa
    20101 aaatgagtta gaggttgaat tcaacaataa aaaagctaga tatttttcaa taagagattc
    20161 cgcagttact ataaataatg aactatcttt acctgatatt aattttgata gcaatgaatt
    20221 atcaacttta ttgtctgaaa aaatcgaaga acctgagttg aattctaggg ataagtattt
    20281 actgacatta ttatctgatt ccacaaaagg tgaacgtcat ttgagagaag ttaaagattt
    20341 ctttgaagat gaacatcaaa aaaaatgtcc tttctgtact caatcagtat cagaagatgt
    20401 taaagttgaa ctcacgaatg gcataactaa actactatca agagcagtcg aagaacacca
    20461 atccgcattg agaggtaaaa aaattgatga gattaatcaa gatttttctg gttatgaaca
    20521 gatagatcca atattaattc agtcttatca aaatagtatt aatgctttga atgctaaatt
    20581 taacgaaatt aactcaataa tcgataagaa gattgataat ccatataata tagttgagtt
    20641 acctaatata agcttttctc aagaattaag tcaagcagag catgatattg aaaaaattaa
    20701 ccaagcaatt atcaaacata attcagaaat ctcgggtatt caaaagttaa aagtagatct
    20761 ccttcaaata aataatgaat tagcttttta tgaaattcaa gatgcataca aaaagtttca
    20821 agagaaaaca aataaaaaag caatttgtga aaataattat aataacagta gtaaacgagt
    20881 aaaggattat gagaagcaga tttctgattt agaagacaag aaacttaata tagatattgc
    20941 agttgatgaa attaataaaa gtttaaatta catatttttt tcaaaaaatc ggttagctat
    21001 acagaatcaa aacggaaaat actatctgtt atcaagaggt aagtctgtgg tccccagtag
    21061 ggtttccgta ggggaacgaa atgcgctggc attatgttat ttctttactg agataattca
    21121 acagagagag ttagcagatg cttatagtca tgaatatttt attgttattg acgatccaat
    21181 ttctagtttt gatatggaaa ataaagttgg aataacctcg tatttaaaat attgtttaac
    21241 tagatttttt aaggggaata gtaatactag agttttacta atgacccatg ataaacaaac
    21301 aatatatgac tttgatatct ttttgaaaga gataatggaa tcctgtaaag aagaggaagg
    21361 tggacaaaaa tctaaataca agaagttaga attggtttct ggtaaattac aagaattcaa
    21421 aactagtact cacgattata cagaactact tgaaatagtc ttcggttatg cattgggaaa
    21481 tagtacacct acttcggaaa gttttgttgg gaatgcaatg agaaaaattt tagaagccta
    21541 tggtagtttt aattataaga agggaattgc cgagctaaca acagatcctt taattgttga
    21601 aaaaattgat aaagaataca gaacttattt tgagaatctt atgtatagac tggttttaaa
    21661 cggtgaaagt cattttaagg atccggttaa aactttgagt attgatttct ttgataccat
    21721 ttctgatgaa gaacgaaaaa aaacagctag agatttacta gttttgcttt atcttttaga
    21781 tgatttacat gttttaaaac atttagaggg tgtatcaaat gcggaaaata gacttgaaca
    21841 atggaagtgt gagattttag aataagagaa tcataccatt tgacttattg caatggttga
    21901 agtttgcatg atagctgatt agagtaatca aaggattatt acttcctaat tttattttct
    21961 acaaacccta atttagcatc taataatgca aagtttcctg agcctcatag ataaaaaaga
    22021 tttttaagga gaaaaacatg atcaaccgtt actctcgccc tgagatggcg aatatttgga
    22081 gtgaagaaaa taaataccgt gcttggcttg aggtggaaat cctctctgac gaggcatggg
    22141 ctgagttggg ggaaatccct aaggaagatg tggctttgat tcgcaagaag gcggactttg
    22201 acatcgaccg tattttggaa attgagcagg agacgcgcca cgatgtggtg gctttcacgc
    22261 gtgcggtttc tgagactctt ggtgaagagc gcaagtgggt tcactatggg ttaacttcta
    22321 ccgacgtggt ggatactgcc tatggttacc tctacaagca ggccaacgac atcatccgtc
    22381 gtgaccttga aaacttcact aatatcatcg ctgacaaggc caaggagcac aagttcacca
    22441 tcatgatggg gcgtactcat ggtgtgcacg ctgagccgac aacttttggt cttaaattgg
    22501 caacttggta tagcgaaatg aaacgcaata tcgagcgctt cgagcatgcg gctgctggtg
    22561 tagaagctgg taagatttct ggtgcggttg ggaactttgc caatatccca ccatttgtag
    22621 aggagtatgt ctgcgataaa cttggcatcc gtgcccaaga aatctctaca caagtccttc
    22681 ctcgtgacct tcacgctgag tactttgcgg tttttgccag cattgcgact tcaatcgaac
    22741 gtatggcgac tgagattcgt ggtctacaaa aatctgagca acgcgaagta gaagagttct
    22801 ttgctaaagg gcaaaaaggg tcttcagcaa tgcctcacaa acgcaaccca atcggttctg
    22861 aaaatatgac tggtctggcg cgtgtcattc gtggtcacat gattacggct tatgaaaacg
    22921 tcgctctctg gcacgaacgc gatatttctc actcatcagc tgagcgtatc atcacaccag
    22981 atacgaccat tttgattgac tacatgctca accgttttgg aaatatcgtc aagaacttga
    23041 cagtcttccc agaaaatatg atccgaaaca tgaactcgac ttttggtctt atctttagcc
    23101 aacgggctat gttgacattg attgaaaaag gcatgacccg tgagcaagcc tatgacttgg
    23161 tgcaaccaaa aacagcctac tcttgggaca accaagtaga ctttaaacca cttcttgagg
    23221 cagattcaga agtaacatca cgtctcacac aagaagaaat caatgaaatc ttcaacccaa
    23281 tttattacac caaacgagtg gatgatatct ttgaacgtct tggactaggt gattaattaa
    23341 aaaataaaca gcgagcttca atctcgctgt ttatttttta tcgaaaagac ttagtcttct
    23401 tttcttttag tgagtccata ggctgctagt gtggacatga gtcctgcgac tactagtcct
    23461 gcagaatcgt gagttcctgt ttcaggaagt tttttctctg ttaccacagg agctggatct
    23521 tgaggaagag ctttgctttc ctcagcagga gcagttgatg gagctggttg gcttgggatt
    23581 tctagtcttg gtttttcttc agcaatagcg gcttgtccgt tttcatcgcc tacatgtgtt
    23641 accatagttc cgacttcgac tatttgagta acggcttcct gtgctacgac actatttaca
    23701 agtgttttca cttccttacc atcggcagaa gtgctcacag agtagaagtt gctacgatgt
    23761 ccattgacgc ccttagtaat gacttgtgtt tttcctttga gtaagagtgg attttcacga
    23821 gtcactgtgg taaatggaat ttcttctttt tggatatcca gtctaggttt tacctcagta
    23881 gttggtgcaa gaccactttc atcacccttg tgagttacag gagcgccaac ttcaaccact
    23941 tggtttataa cttctttggt tacctggcta tcaaggactg tttctgttgt ttttccattt
    24001 tcagtgagta cagagatgta atgagttcgt tcacctttga ctcctgctgt gataatattt
    24061 tcctgaccag ctgggaggtt aggattttct ttcttgataa cttcaaatgg aatttcttca
    24121 gttcttgtga tgagttctgg tctggtttca acattggcag ccacttcatt ttcatccagg
    24181 cttcctgaat gagttgcagc tggtttgagg ccttgaagag cggcttttag gttggctaca
    24241 agtgtgtcaa gctcagcttg tttattacgg ttgaggttgt aatttagagc tgttttagct
    24301 gcgtcaaggg cctcaagact ttctttacta tatccttcta agtttgtagg aattttagct
    24361 aattcttcgc ggagagcatt ataattagca cggaagtagt ctttgttgtg gtctgcaaag
    24421 gcagtcatga gttcaaagat ttcctcttcc ttgtactcag cgcttggtct atctgcccag
    24481 attgaaagca tacttccgac tgttggaaga tctacttcag gatatttggt agaagctagt
    24541 tgattgaatg gaactttacc agaattttca actgcttttg aaagagggta agcttcatct
    24601 tgtttgtgat tgcctataac ataataccag tcaccgttgg tattcaagaa tttatagcct
    24661 ttgcttgcta ggtattgagg tgatgcgagg ttatatcccc accagccttt agaccagtaa
    24721 gaaatcaaga catctttgtc aaactgaaca tcgtccttgt cttcatagta gaagccatcg
    24781 ttgaaggcca ttggttgaag ccctctttct ttggccatag ctgcgagggt gttggcatat
    24841 tcggcaaatt tgccatagag ttgataccac ttgaggtagt accagccttg ggcactagtc
    24901 gcatcgttgg cgtattcgtc agtaccaaag ttgaaaatct ttgttttacc tgcaaagaag
    24961 tccatgtatt taccgatgag ggcttttaca aagttcatcg cttcttcgtt tttcaagtcc
    25021 atagttgttt ttgaaacttt atcaaagtgg gcttgaggat ttttaatacc taatttttcc
    25081 atggcaacca gcatagcatc catgtgacct ggactgttaa tagctgggat gagaccaatg
    25141 tccttagatt tagcgtattc aattagctct gtcacttctg cctgtgttag tgcagtaccg
    25201 tttggatcgt cgtagtaagc tttagttcct tcgataatag cttttttaac gtcatcacta
    25261 gcataggttt ttccgttggc agtaatggtc atatcattga gtagaaagcg aagtccgtca
    25321 tttcctagaa ggagatggac atcagaatat ccgagctcac tggccttgtc tacgatgcgt
    25381 ttgagctggt ccagagtaaa gtatttgcgt ccagcatcga ttgagattac cttgtttttg
    25441 gcaagttttt caacctcacg tttagcttct tcttcttttt gagcttcagg cgtgagggtc
    25501 aagttgttga cagtttcttg aagtttagca atggcttgat caatcgtatc ttgttgggca
    25561 cggctaaggt tgctatcgag agagcgaata gctttttcag cttcttttac ggccgcgacg
    25621 ctttctgcag tataacggtt caggtctttt ggtacctcgt taagtgcttg ctctgcagat
    25681 tcataatcag ctgcgaagta ttcagcgttg gcatttgcaa aatgacgcat gagtttgaag
    25741 aggtgtgatg gtgaataacg tgcagatgga gtgtcagccc aagcagctac cataccaccg
    25801 atgattggga tatcagctcc ttctgttttt ggtacagaag tgattggtgt gtttttaata
    25861 ccattgagcc cctgatcgag attgtaccag ccttggccat cagcgtttcg tccaagaacg
    25921 tagtaccaag catcattggt attaaggatt tggtgacctt tttcagctag tagtttagaa
    25981 gaagcgacat cgtagcctcc ccaaccacca gtccacatag aaacgatgat gtctttgtca
    26041 aaactaccaa agcttgtgtc gctattgtag tagataccgt cgttaaaggc cattggtttg
    26101 agaccgtgcg attttacaat acgagcgagg tcattggcgt aggcaataaa tttttcatag
    26161 ccttttacag ggtagccttc gtttggatag tatttatcag cttgaagcac actccaacct
    26221 ttagcatctg tcgcatcatt ggcatattca tcaagtccga tgttgaagat ttcagtcttt
    26281 ttcgcgaaat aagcagcata cttgtcgata agggcttttg taaaagcgac agcttgttcg
    26341 ttgtcaagat cgacagtacg ggctgatttc ttcccaaaat agctaaagtt agggttttgg
    26401 attcccaatt ctttcatggc attgagaatc gcatccatgt gtccaggact atttactgtc
    26461 ggaatgagac cgataccttt atctttggca tagttaatca gatctgtcat ttgactttct
    26521 gttaagtgat tgccgtttgg atcgttgtaa taatcatttg tacctttttc aatggcgcgt
    26581 ttgacatcgt cactggcata ggtcttgccg ttagctgtga tgctcatatc gtccaacatg
    26641 aaacggagtc catcatttcc gactaatagg tgtaaatcag tgtagccata atgtttcgct
    26701 ttatcgatga tttccttgag ctgttctggt gagaaatatt tacgtccagc atcaatagaa
    26761 acaattttct ttttcgctag tttttcattt acagttgcag cacgttcctt tcctgtctct
    26821 gtttccggtt tgtcagcctc tgctttcgct tcatcttttt tagctggttt atccttgtca
    26881 gtcttgtctg tatttgactc tttagaatca acctctttcg cttcttcctt tttagggcta
    26941 gcttcttctt cctttttatt agcagtttct ttttcagcag aagttggagt taccacctct
    27001 gctttatcac taggagttga actaacttcc tcttgtggtt tttcttctgt ttttggaaga
    27061 ctagctacct tatcagtagc tggagtttct gtttctacag tttttggagc ttctggttga
    27121 agctctgctt taggtgtttc ctcagtccga ttttcggatg attgagggga atcagaaacc
    27181 gtatggatgg tcggttggtt ttctgtagta gtaggagtaa ctccatcggc tgcaacagtc
    27241 tgtgcttgga aggcaaatcc aattagaaca gaagctgctc ctacagcgta tttacgaata
    27301 gaaaaacgct gttgtttttc atgtttcatt gcaaaacctc ctgattgcat tgttatattg
    27361 atagcgatta tataaatcaa cgcctttatt ttatttctta tattaacgag agtcaagagg
    27421 agatgacaaa aaactataat aagtataaaa aaatataaaa tttaaactta agatttcaga
    27481 ttggtcgaaa aaatacatat atatatctag tataattttt ggttctattt ctataaaata
    27541 ttccacaaat tatagaatct tccaaaaata ggtaagcgct acctttttgg tgtggtataa
    27601 taagcataga aaaagcccga gcgattaggc tcaggctttc ttcttagtga tcacggtcac
    27661 atgagatgaa tttaatcttg tagtaatcag atcgtttgta agtttcactg tattctaaaa
    27721 cttggccagt tgattcgagt ttggtgattt tagtttgtag gacagtaggg aattgttcat
    27781 cgactccgag gactgaagcc gcatgttctg gagttggaaa gactatttcg ttgatttctt
    27841 caaagtgttc atcattcatg tgaatgtggt agtctaactt gaaacgatta tagatagaac
    27901 tatagtattc aaggtttgga taatttgcgt tgatatattg ttctgggatg taggatgtat
    27961 ggtagatata aacgacaccg tttgattcgc ggatacgttc aatcttgtag tagaattgat
    28021 cgccgcgtag acccaatttt tccaagtaaa caagcttgtt tccgcgttca attgaaagaa
    28081 cagttacctt atcatcttta gcattgaaga gttcaatatc tgaaaactct acaagcttgt
    28141 gtttgcgtgc acgtgaaacg aaggttcctt ttccttgttg gcggacaata tagccatctt
    28201 tggcaaggtc gtttaaggcg cgaacaactg tgatagagct gacatcgtac attgaaatga
    28261 gttctgcttc agtgtaaaat ttatctccac tgctaaactg cccagagatg attttatttt
    28321 ttaattcgtc ttttatgtat tgatatttgg gaattgccat atttttacct ctattttcct
    28381 tctccataga aaattttacc ataaaagcga aaaaaatagt agtcttttga ataaaaaatt
    28441 agaataatag tttgaaaaat taagcgatat agggattgct catattttat caatgcattc
    28501 tatacaaaat tattttaggt cctatattcc gaatttaaag agcgtttgaa atgggtctaa
    28561 aaatgtttga aactaattga atgtgtgtat aatagtaaaa atgcagtaaa tatcccgaca
    28621 atttctattg acaattcaaa cagattggtt tataattaat ataacaacaa atgaaagcgc
    28681 aaactttcgc ggtcggaagg tagttttatg acacgatttg agatacgaga tgatttctat
    28741 ctcgatggaa aatcatttaa gattttatct ggtgccattc attattttag gattcctcca
    28801 gaggattggt atcattcgct ctataacttg aaggctcttg gttttaatac ggtagagact
    28861 tatgttgctt ggaatttaca cgagcctcgt gaaggtgagt ttcattttga aggtgatctg
    28921 gatttagaga aatttctcca aatagcgcag gatttgggtc tctacgcaat tgtgcgtccg
    28981 tctccattta tctgtgcgga atgggaattc ggtggcttac cagcttggct cttgaccaag
    29041 aacatgcgaa ttcgctcatc cgacccagca tatatcgagg cagttggtcg ctactatgat
    29101 cagttattgc caagactggt gcctcgtttg ttgaacaatg gtggcaatat tctcatgatg
    29161 caggttgaaa atgagtatgg ttcttacgga gaagataagg cttacctgag agcgattcga
    29221 cagctaatgg aagagtgtgg cgtaacctgt cccctcttta catcagatgg tccatggcga
    29281 gctactctga aagctggaac cttaattgaa gaggacctct ttgtaacagg aaactttggt
    29341 tctaaggcac cttacaactt ttcgcagatg caggaattct ttgatgaaca tggtaagaaa
    29401 tggccactca tgtgtatgga gttctgggat ggttggttca atcgctggaa agaaccgatt
    29461 atcacacggg atcctaagga attggcagat gcagttcgag aggttttgga acaaggctct
    29521 atcaatcttt acatgttcca cggtggtaca aactttggtt tcatgaatgg ttgctcagct
    29581 cgaggaactt tggacctgcc acaagttaca tcttatgatt acgatgccct tctggatgaa
    29641 gaaggaaatc caactgctaa atatcttgca gtcaagaaga tgatggcaac acatttttca
    29701 gagtatccgc agttggaacc actctacaaa gagagtatgg agttggatgc tattccacta
    29761 gttgaaaaag tttctttgtt tgaaacctta gatagcttgt caagtcctgt agaaagtctc
    29821 tatcctcaaa agatggagga gctgggacaa agttatggct acctacttta tcgaacagaa
    29881 acaaactggg atgcagaaga agaaagactt cgtatcattg atggtcgaga tagggcccag
    29941 ctgtatgtcg atggtcagtg ggttaaaact caatatcaga cagagattgg ggaagatatt
    30001 ttttatcaag gtaaaaagaa agggctatct aggttagata tcttgataga aaatatgggg
    30061 cgtgtcaact atgggcataa gttcttagcg gatacgcaac gtaagggaat tcggacaggg
    30121 gtctgtaagg atctgcattt cttactaaac tggaaacact atccactccc actagacaat
    30181 cctgagaaaa ttgatttttc aaaaggatgg actcaaggac aaccagcctt ttacgcttat
    30241 gactttacag tcgaagagcc aaaagatact tacctagact tgtctgagtt tggtaaggga
    30301 gttgcctttg tcaatgggca gaatctagga cgtttttgga acgttggccc aactctctca
    30361 ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc gtatcattat ctttgaaaca
    30421 gaaggtcaat ataaagaaga gattcattta actcgtaaac ctacactaaa acatataaag
    30481 ggggaaaact tatgacaatt gtaggatgcc gtattgatgg acgtttgatc cacggacaag
    30541 tagccaatct ttgggctgga aaactaaatg tttcacgcat tatggttgta gacgacgaag
    30601 ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc gacaccacca ggtgtgaaat
    30661 tgagtatttt gccagttgag aaagctgcag ccaatattct tgctggtaaa tacgatagcc
    30721 aacgtctctt tatcgtggct cgtaaaccag accgcttcct tggtttggta gaagcaggtg
    30781 taccacttga aacccttaat gttgggaata tgtctcaaac accagaaact cgttctatta
    30841 cacgttctat caacgtagta gacaaggatg tggaagactt ccacaaactg gcagaaaaag
    30901 gtgttaaact tactgctcag atggttccaa atgatccaat ttcagacttt ttgagcttat
    30961 taaaatagga aaaaaatttt taggaggtca ttgttatgat acaatggtgg caaattttac
    31021 ttctcacttt gtactcagct tatcaaatct gtgatgagtt gacgatcgtt tcatctgcag
    31081 gttcccctgt atttgctggt ttcattactg gtttaatcat gggagatgtg actactggtt
    31141 tacttatcgg tggtaacttg caactgttcg ttcttggggt tggtaccttc ggtggtgctt
    31201 ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc cttctctgtt tcacaaggaa
    31261 ttgatgcacc gcttgccatt actacaatcg ctgtaccagt agcagctctc ttgacttact
    31321 tcgacgttct tggtcgtatg actactacct tcttcgctca ccgtgtggat gctgcaatcg
    31381 aacgctttga ctataaaggt attgaacgca actacttgct tggtgcgatt ccgtgggctc
    31441 tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg tggtgccttt gtacaatcag
    31501 tagtagactt cgttgaagcc tacaaatggg ttgcagatgg cttgacactt gcaggacgta
    31561 tgcttccagg tcttggattt gcaatcttgc ttcgttacct tccagttaaa cgtaaccttc
    31621 actaccttgc tatgggattt ggtttgacag ctatgttgac tgttctttac tcatatgtaa
    31681 caggtcttgg tggcgctgtt gctggtatcg taggtactct tcctgctgaa gttgctgaaa
    31741 aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat tggtatttct atcgtaggta
    31801 ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt agctgtagca gcaccttcta
    31861 caccatcaga aagtggggaa atcgaagatg acgaattcta attacaaact tacaaaagaa
    31921 gattttaatc aaatcaacaa acgtagcttg tttactttcc aattaggttg gaactacgaa
    31981 cgtatgcaag cttctggtta cctttacatg atcttgcctc agttgcgtaa aatgtatggt
    32041 gatggaactc ctgaattgaa agaaatgatg aaagttcata ctcaattctt caatacttca
    32101 ccattcttcc ataccattat cgctggtttt gaccttgcca tggaagaaaa agatggtgta
    32161 ggttcaaaag acgccgttaa cggtatcaag acaggtttga tgggaccatt cgctcctctt
    32221 ggggatacaa tctttggttc acttgtacct gctatcatgg ggtcagtcgc agcaactatg
    32281 gctatcgctg gccaaccttg ggggatcttc ctttggattg cagttgcagt agcgtatgac
    32341 atcttccgtt ggaaacagtt ggaatttgct tacaaagaag gggttaacct tatcaacaac
    32401 atgcaaagta ccttgacagc tttgattgac gctgcatctg tacttggtgt cttcatgatg
    32461 ggtgctcttg tagcaacagt gattaacttt gaaatttctt acaagttgcc aatcggtgaa
    32521 aagatgattg atttccaaga catcttgaac caaatcttcc cacgtttgct tccagcaatc
    32581 tttactgcct ttatcttctg gttgcttggt aagaaaggta tgaactctac taaagctatc
    32641 ggtattatta tcgtacttgc tttggctctt tctgcccttg gtcactttgc acttggaatg
    32701 taattcctta tgactaaatc attaattttg gtgagccatg gtcgcttctg tgaggagctt
    32761 agaggtagca cagaaatgat tatgggccca caagacaaca tttacacagt agctcttctt
    32821 ccagaagatg gcccagaaga atttactgct aaatttgaag ctgttattga aggattggat
    32881 gatttcctag tctttgcgga tcttctcggt gggacacctt gtaatgtggt gagtcgcttg
    32941 atcatggaag gtcgtgatat tgacctttac gcagggatga atcttccaat ggtgattgaa
    33001 tttatcaatg cgagccttac aggcgcagat gcggactaca agagccgtgc tgcagaaagc
    33061 attgtgaaag ttaatgacct gttagcgggc ttcgatgatg acgaagatga ataatactct
    33121 tcgaaaatct cttcaaacta cgtcaacgtc gccttgccgt aggtatatgt tactgacttc
    33181 gtcagtctta tccggcaacc tcaaaacggt gttttgagct gacttcgtca gtcttatccg
    33241 gcaacctcaa agcagtgctt tgagcagcct gcggctagtt tcctacagat tttagttgga
    33301 actcgattca attcatgtga caacgtgaaa atcgttagag cattttatat agaatataca
    33361 tgggaatgta gcttactccc attcccatat ttaatagaaa aagaggaact caatgctaca
    33421 ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc actacgcgtg aaatctacca
    33481 acagcctgat gtatggagag aagcttttga attttatcaa gcaaaacgtg aagaaattgc
    33541 agccttccta caagaaatcg ctgataaaca tgactatatt aaggttatct tgacaggtgc
    33601 tgggacttct gcttatgtgg gagatacctt gctaccttat tttaaggaag tctatgacga
    33661 acgcaaatgg aatttcaatg ctattgcgac aacagatatc gttgccaatc cagcaaccta
    33721 tttgaaaaaa gatgtggcaa ctgtccttgt gtcttttgct cgtagtggga attcgcctga
    33781 aagtttggcg actgttgatt tggccaaatc cttggtggat gagctttatc aagtgacgat
    33841 tacttgtgca gcagatggta aattggctct tcaagctcac ggtgatgatc gtaatctctt
    33901 gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc atgacttcta gctttacgtc
    33961 tatgatgttg acaactctct tggtctttga tcctacagaa tttgctgtta agtctgaacg
    34021 ttttgaagtt gtatctagtc ttgcccgtaa agttttagac aaggcagaag atgtcaaaga
    34081 gctcgttgat ttagacttta accgtgtcat ctatctaggc gctggtcctt tctttggact
    34141 tgctcatgaa gctcagctca agattttgga attaactgct ggtcaagttg cgaccatgta
    34201 tgaaagccca gttggcttcc gtcacggtcc aaaatctctt atcaacgaca atacagttgt
    34261 tttggtcttt ggtacaacga cagactacac tcgtaagtac gacttggact tggttcgtga
    34321 agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg agtgatcaag cttttggtct
    34381 tgaaaatgtc aaagaagtgg cccttggttg tggcggtgtc ttgaatgata tttaccgtgt
    34441 cttcccttac atcgtttatg cccaactctt tgctttattg acttcactca aggtagaaaa
    34501 taaaccagat acaccgtctc ctacaggtac agtaaaccgt gtagtacaag gtgtcataat
    34561 tcacgaatat caaaagtaag acagtgttta tgaattcttg acaagaggat ttgtaaatta
    34621 tcagataaac catagattgt cagtacgctt tctatggttt gtttgcttga gagaaatagt
    34681 aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt tggaaatgtt gagggtgagg
    34741 atgtcttggc ctatcgattt gagacagacg gtggctacca acttgaggtt atgacttatg
    34801 gtgcgactat cttgcgctat gtcgcacctg acaaggctgg aaattttgcc aatgttatct
    34861 tgggatttga tgactttgat agttatgtag gcaatagtcc caagcatgga gcaagtgtag
    34921 gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct caatggtaag acctatgacc
    34981 ttgaggttaa taatgctagc aactgtaatc acagtggttc aactggttgg gattccagct
    35041 tgtttgaagt tgaagaagta agcgatcatg gcttgactct ctacacagag cgtacagatg
    35101 ggacaggagg gttccctgga aatctcaaga tttggatcag ttatcacttg gaagaaactg
    35161 gtgcctatga aatcagctac aaggtaacga ccgatcagga tacgctggtc aatccaacca
    35221 accacagcta tttcaacttg tctggtgatt tcacgcagac gattgaccgt catgtcttcc
    35281 aactaaacac agagggcatt tactcaatcg ctcctgacgg tgttcctgcc aaaactccag
    35341 aagccaaccg tgatgtggtc aaacacgtct acaatggtac cttgttgaag gatatctttg
    35401 cagaagaaga tgagcaaatc cagctggcat caggtttgga tcatccattt gcccttcctg
    35461 caggccatga caatgctgga ttcctttatg accaaaattc aggtcgcttc ctgcttttca
    35521 agacagaagc tccttgcttt gtggtctaca cagcaaactt tgtggatgaa agtgtcatca
    35581 taggaggtca gccaatgcta cagcacaatg ggattgctct tgaagcgcaa gctttaccag
    35641 atgccattca cagtgacctt aaaggccaag tcattcttaa agctggtcaa accttcacca
    35701 gtaagacacg ttatgaactt gttgtgaaat aaaaaaagct ggtcaaaaag tctttaaaat
    35761 taaaataacg catattgtca ggtgatgaaa agtttgataa tatgcgtttt attatgaaat
    35821 gatttacttt atattatcct gaaatggtgt tatttttgag ttatctggct tgactttctt
    35881 gatggaaatt atataatagt tgtaacaata ataattacaa ctagaaaaga ggaccattta
    35941 tgacctatga atacaagagt cacatttatt tggcagagac agctttaaat gtaaaggatt
    36001 tggccagtca aacagccttt tatcagcaag ttattggttt agaaatctta tctcaaactg
    36061 agacagagtc cattctaggc cttggtggaa aagtcttggt acagctgatt caagcacaag
    36121 agagcggaga agtgagggaa tattatggtc tttaccattt ggccattctc ttgcccacac
    36181 gcaaggcttt agctgatgtc ttgaaacacc tgacagattt acaaattcct cttgtcggcg
    36241 gtgcagacca tggttacagt gaagctattt acttggaaga tttggaggga aatggcattg
    36301 aactctatcg agataagcca gtttccacat gggatattcg agaagatgga cgcattatcg
    36361 gagtgacgga ggctcttgcg gcgcaggaca tttatgagtt gggggaaaga gtagagccct
    36421 ttatcctggc agaaggtaca agaatggggc atattcatct ttccgtcaag gatagtcgaa
    36481 agtccagcca gttttatcaa aaggtgttag ggctagagaa taaattcagt gtgcctggcg
    36541 ctagttggat tgcggctgga gattaccatc atcatttagc agttaacgaa tggggaggaa
    36601 aaggtctggc ttcgcgtaag caaggcttac caggtttagc ctactatgtc atcgaagtcg
    36661 cacataaaaa agaactgtta acgattgccc aacgagcaca agaagttgac gcaccaatca
    36721 aatggatgac atcgatccaa ttggaaatca cagactcaga tggcatcgtg acccgtattc
    36781 gtttagcaag gtaaaattag gaatttttaa taagttataa gtattgctag tttgcttttt
    36841 gaacggtctt tggtaacaga actgcgtgag aattttgaca aagaagctga ttagagatat
    36901 aatgaagaaa aatcgagcaa ggaaaaagaa ctgacaaatt caaagtatat tacttgtttg
    36961 aaacgttcag agggccagtt gtgtgggatt caaaagatga ttgaaggaga tcgtgactgt
    37021 gctgatattg tgacgcaact gacagcagtg aaatctagtg tagagcgcgt gattgagatg
    37081 ataattaccg aaaatcttac tgaatgtatc aatcagcccc tagatgattc tgaagctcaa
    37141 aaggaacgcc tagaaaaggc tatccgatac ttgattaaac ggaaataaat gaatgttttg
    37201 agacgatatg tgcaaaggtc tattaactat tagaatagtt taatgtgagt caaaagaagt
    37261 tgtaaaaaat ctctaaaatt aaaaaaacgc atagtatcag gtgttgaatg tactgacccc
    37321 caaaagttag attttttctg tctaactttt ggggtgcagt tcataagaac cttggtaata
    37381 tgcgttttat tatgtagaga cttatactct tcgaaaatct cttcaaacca cgtcagcttc
    37441 accttgccgt agatatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt
    37501 tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga gcagcctgcg
    37561 gctaacttcc tagtttgcac tttgattttc attgagtatt acttcatctt ttcctgaaat
    37621 tgagtttttg cccaactcct cttgattatt ctacttgacc gggccaagca ttcataccac
    37681 cttctacgtt ggtaacggtg aggccttggg agctgagaaa ctggcaggta gatgcagagc
    37741 ggactcctcc ttgacaaatg acatagtatt catggtcagg tttgagttct ttgtagcctt
    37801 gctctaaggt acttaatgga agatttttgg cacctggtgc atgtcctgct tggaattcat
    37861 gtgcttcacg gacatcgata agttctagat tttcattttg gtattttcca taaaagtcag
    37921 ccatgctgat actagtttcc atattctact ccttctgggt taaccatttt gtatagtgaa
    37981 taagcgccgt caaggttttg ggcggtaaat cctgcttgct tgaggatagg ctctgcgata
    38041 tagctgcgca aaccactgtg gcagctgacg atgtaggctt gttttttgtc cagttcatca
    38101 gtttcctcct tgacactttg tcagtttttg atacaatagt acaaaattag aggaggcagg
    38161 ctatgattca gaaacatgcg attcctattt tagagtttga tgacaatcct caggcggtta
    38221 tcatgcccaa tcacgagggg ctggacttgc agttgccaaa gaagtgtgtt tatgcatttt
    38281 taggtgagga gattgaccgc tatgcaaggg aagtaggggc ggattgtgtc ggcgaattcg
    38341 tttctgctac caagacctat ccagtctatg tcatcaacta caagggtgag gaggtctgtc
    38401 tggctcaggc tcctgttggc tccgctccag cagcccagtt tatggattgg ttgattggct
    38461 atggtgtgga gcagattatc tctactggga cctgtggtgt cctagctgat atagaggaaa
    38521 atgcctttct agtccctgtt cgcgctttgc gagatgaggg agccagttac cactatgtgg
    38581 caccttgtcg ttatatggaa atgcagccag aggctattgc tgctattgag gaagttttgg
    38641 aagacagagg gattccttat gaagaagtca tgacctggac gacagacggt ttttaccgag
    38701 aaacggctga aaaggtggct tatcgtaagg aagaaggctg tgctgttgtg gagatggagt
    38761 gttctgctct tgcggcagta gctcaattgc gtggggttct ctggggtgaa ttgttgttca
    38821 cagcagattc tctagcggac ttggaccagt acgacagtcg tgactggggc tcggaagctt
    38881 ttaataaggc gctagaactg agtttagcaa gtgttcacca cctttagttg tactggcaaa
    38941 ggatttgttt tatcataaaa tgtctagctc atacttttca aaaatatgtt taaacgaggt
    39001 caccttcctc ttgtcctagg catgttgagg ttgggaaaaa tctttaaaat cagaaaaacg
    39061 tatcatatca ggtgatgaaa attttgatac tatgcgtttt atgttggtaa gatttagagt
    39121 gagatgaaat gatactcttc gaaaatctct tcaaaccagg tcagcttc
//
LOCUS       DAVMMR010000015        38190 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMMR010000015 DAVMMR010000000
VERSION     DAVMMR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.015"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            83..445
                     /locus_tag="VYS74_000734"
     CDS             83..445
                     /locus_tag="VYS74_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788801.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGRILA
                     IIGGSLFLATLKKFKSEE"
     gene            980..1672
                     /locus_tag="VYS74_000735"
     CDS             980..1672
                     /locus_tag="VYS74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV3788802.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1725..4112
                     /locus_tag="VYS74_000736"
     CDS             1725..4112
                     /locus_tag="VYS74_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV3788803.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNSDYHLNVNLQMNY
                     WPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHTQATPFGWT
                     APGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFWNAFLHKDQ
                     QAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLLTEVKEKSD
                     LLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSYKGQEYIEA
                     ARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLWCSHPPFQI
                     DGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSMSWEDKKLL
                     QLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDLVQFYF"
     gene            4362..5063
                     /locus_tag="VYS74_000737"
                     /pseudo
     CDS             4362..5063
                     /locus_tag="VYS74_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            5065..7773
                     /locus_tag="VYS74_000738"
     CDS             5065..7773
                     /locus_tag="VYS74_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV3788804.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYMVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7869..8360)
                     /gene="tpx"
                     /locus_tag="VYS74_000739"
     CDS             complement(7869..8360)
                     /gene="tpx"
                     /locus_tag="VYS74_000739"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV3788805.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8483..9412)
                     /gene="psaA"
                     /locus_tag="VYS74_000740"
     CDS             complement(8483..9412)
                     /gene="psaA"
                     /locus_tag="VYS74_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV3788806.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9444..10292)
                     /locus_tag="VYS74_000741"
     CDS             complement(9444..10292)
                     /locus_tag="VYS74_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3788807.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10289..11011)
                     /locus_tag="VYS74_000742"
     CDS             complement(10289..11011)
                     /locus_tag="VYS74_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3788808.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            11302..13194
                     /gene="pepO"
                     /locus_tag="VYS74_000743"
     CDS             11302..13194
                     /gene="pepO"
                     /locus_tag="VYS74_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV3788809.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(13317..13946)
                     /locus_tag="VYS74_000744"
     CDS             complement(13317..13946)
                     /locus_tag="VYS74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3788810.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(14286..14528)
                     /locus_tag="VYS74_000745"
     CDS             complement(14286..14528)
                     /locus_tag="VYS74_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788811.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            14939..17161
                     /locus_tag="VYS74_000746"
     CDS             14939..17161
                     /locus_tag="VYS74_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV3788812.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALIYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17191..17634
                     /locus_tag="VYS74_000747"
     CDS             17191..17634
                     /locus_tag="VYS74_000747"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV3788813.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTDIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(17673..18088)
                     /locus_tag="VYS74_000748"
                     /pseudo
     CDS             complement(17673..18088)
                     /locus_tag="VYS74_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18446..18808
                     /locus_tag="VYS74_000749"
     CDS             18446..18808
                     /locus_tag="VYS74_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788814.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            18989..19288
                     /locus_tag="VYS74_000750"
     CDS             18989..19288
                     /locus_tag="VYS74_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788815.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(19383..19820)
                     /locus_tag="VYS74_000751"
     CDS             complement(19383..19820)
                     /locus_tag="VYS74_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788816.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(19922..20587)
                     /locus_tag="VYS74_000752"
     CDS             complement(19922..20587)
                     /locus_tag="VYS74_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV3788817.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            complement(20775..21425)
                     /locus_tag="VYS74_000753"
     CDS             complement(20775..21425)
                     /locus_tag="VYS74_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV3788818.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            21537..22379
                     /locus_tag="VYS74_000754"
     CDS             21537..22379
                     /locus_tag="VYS74_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV3788819.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMNFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            22455..22892
                     /locus_tag="VYS74_000755"
     CDS             22455..22892
                     /locus_tag="VYS74_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV3788820.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            22961..23263
                     /locus_tag="VYS74_000756"
     CDS             22961..23263
                     /locus_tag="VYS74_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788821.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLDFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(23354..24427)
                     /locus_tag="VYS74_000757"
     CDS             complement(23354..24427)
                     /locus_tag="VYS74_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV3788822.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDAELQLYFDLFFGTILDAGISSINDLTSLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            24583..25260
                     /locus_tag="VYS74_000758"
     CDS             24583..25260
                     /locus_tag="VYS74_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788823.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            25253..26227
                     /locus_tag="VYS74_000759"
     CDS             25253..26227
                     /locus_tag="VYS74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3788824.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(26276..28219)
                     /gene="thrS"
                     /locus_tag="VYS74_000760"
     CDS             complement(26276..28219)
                     /gene="thrS"
                     /locus_tag="VYS74_000760"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV3788825.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LEHYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(28277..28453)
                     /locus_tag="VYS74_000761"
     CDS             complement(28277..28453)
                     /locus_tag="VYS74_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV3788826.1"
                     /translation="MTKQGRYYDETSYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(28460..28600)
                     /locus_tag="VYS74_000762"
     CDS             complement(28460..28600)
                     /locus_tag="VYS74_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV3788827.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(29031..29231)
                     /locus_tag="VYS74_000763"
     CDS             complement(29031..29231)
                     /locus_tag="VYS74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788828.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            29470..30099
                     /locus_tag="VYS74_000764"
     CDS             29470..30099
                     /locus_tag="VYS74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3788829.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKEHISLLEK"
     gene            30240..30509
                     /gene="rpsO"
                     /locus_tag="VYS74_000765"
     CDS             30240..30509
                     /gene="rpsO"
                     /locus_tag="VYS74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV3788830.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            31325..31939
                     /locus_tag="VYS74_000766"
     CDS             31325..31939
                     /locus_tag="VYS74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV3788831.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(32231..32980)
                     /locus_tag="VYS74_000767"
     CDS             complement(32231..32980)
                     /locus_tag="VYS74_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV3788832.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            33113..35449
                     /locus_tag="VYS74_000768"
     CDS             33113..35449
                     /locus_tag="VYS74_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV3788833.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            35550..36227
                     /locus_tag="VYS74_000769"
     CDS             35550..36227
                     /locus_tag="VYS74_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV3788834.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            36214..37011
                     /locus_tag="VYS74_000770"
     CDS             36214..37011
                     /locus_tag="VYS74_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV3788835.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            37028..38131
                     /locus_tag="VYS74_000771"
     CDS             37028..38131
                     /locus_tag="VYS74_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV3788836.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
BASE COUNT        11225 a         7087 c         8718 g        11160 t
ORIGIN      
        1 ttataatatc agaaaatact ttttttaaaa agcaaatatg atacaatttt atttgaaaaa
       61 aaataaaaaa ggagatttta ttatgaaatc aagtggtatc gtcggtcttg tgggaggaat
      121 tttacttctt atttgtcctt ttgtcttgtt gggaacagcg gtaaatacag ctgctacaac
      181 tcttaatgga ggagctactg caggggcttt ttcaggtgtt gccttactct tgaatgcctt
      241 gaagattgca aatcttgttc ttggtatcat tgctattgtt tactataaag gagataagca
      301 tgtaggtgca gctccgtctg tactaatgat tgtttctggt gaagttagtc tcattccatt
      361 cttaggatgg gttgggagga ttcttgctat tatcggagga tctctattcc ttgcaacatt
      421 gaagaaattc aaatcagaag aataaaaggt attttagcat gaaaagaaca aaaaagttta
      481 tcggtatagg agtagctcta ttatctcttt ctcttctagt tgcatgtgga acataaagtt
      541 caaagaatac ttcaacaagt aatgatgaga agacagtagc aacatccaat agttcaaaag
      601 aaacaatcac tttcgataca ccggttgtaa cagacgatgc gattgaatca atacgcactt
      661 atgcagatta tatagatctt tataaaaata tttttgatga ttattttact aaagctgagg
      721 aaggtttcaa aggcacaact atggaaaata atgactcgtt tactaaacta aaagagtcaa
      781 ctcaaaaatt attcgatgcg cagaaaaaaa ggttaaataa tgaagataga atagaaacaa
      841 ccaaaaacaa tgtgattgcc aaacattgtc aaacagtcct ttcctttttg gttttgacta
      901 gcttttttgt gaaaaattgt gtaaaataga atagataaac gaggggaaac ctcggaaaat
      961 ttaaaggaga atccatctaa tggtaaaatt ggtttttgct cgccacggtg agtctgaatg
     1021 gaacaaagct aaccttttca ctggttgggc tgatgttgat ttgtctgaaa aaggtacaca
     1081 acaagcgatt gacgctggta aattgatcaa agaagctggt atcgaatttg accaagctta
     1141 cacttcagta ttgaaacgtg ctatcaaaac aactaacttg gctcttgaag cttctgacca
     1201 attatgggtt ccagttgaaa aatcatggcg cttgaacgaa cgtcactacg gtggtttgac
     1261 tggtaaaaac aaagctgaag ctgctgaaca atttggtgat gagcaagttc acatctggcg
     1321 tcgttcatac gatgtattgc ctccaaatat ggaccgtgat gatgagcact cagctcacac
     1381 agaccgtcgt tatgcttcac ttgacgactc agtaatccca gatgctgaaa acttgaaagt
     1441 gactttggaa cgtgctcttc cattctggga agataaaatc gctccagctc ttaaagatgg
     1501 taaaaacgta ttcgtaggag ctcacggtaa ctcaatccgt gcccttgtaa aacacatcaa
     1561 aggtttgtca gatgatgaga tcatggacgt ggaaatccct aacttcccac cattggtatt
     1621 cgaattcgat gaaaaattga acgttgtttc tgaatactac cttggaaaat aaaaaattgt
     1681 aagcctagga ttgatttcta ggctttttat gttagtatgg aagtatgata aggaataaaa
     1741 aacaagatta tgtactggcc tacaagcaac cagcttcaac cacttacatg ggttgggaag
     1801 aagaagcttt accgataggt aatggttctt taggagcaaa agtatttggc cttatagggt
     1861 ctgaacggat tcaatttaat gaaaaaagtc tctggtctgg aggtccactt cctgatagtt
     1921 cagattatca gggtggaaat cttcaggatc agtatgtttt tttagctgag attcggcagg
     1981 ctttggagaa gagagattac aatctggcta aggaactggc tgagcagcac ctaattgggc
     2041 caaaaacgag tcaatatggg acctatctgt cttttgggga tattcacatt gagttcagcc
     2101 agcaaggtac gactttgtct caggtgacgg actatcagag acagttgaat attagtaagg
     2161 cacttgcgac gacttcttat gtctataagg gaacgcgatt tgaacgtgaa gcttttgcga
     2221 gttttccaga tgatctcttg gttcaatgtt ttactaagga agggttggaa actctagatt
     2281 ttactataga actatccttg acctgtgatt tggcttctga tggaaagtat gagcaggaaa
     2341 aatctgatta caaggagtgt aagttggata ttactgattc tcatatcttg atgaagggaa
     2401 gagttaagga taatgatctg cggtttgcta gttatctagc ttgggaaacg gatggagata
     2461 ttagagtttg gtcagatagg gttcagatat caggagccag ttatgccaat ctcttcttgg
     2521 ccgctaagac ggattttgcc caaaatcctg ctagcaatta tcgcaagaaa ctagatttag
     2581 agcaacaggt gatagacttg gtggacacag ctaaagaaaa gggctatacc caattgaaat
     2641 caaggcatat cgaggactac caagccttat tccagcgtgt tcaattggat ttggaagctg
     2701 atgttgacgc atccactaca gatgatttgt taaaaaatta taagccacaa gaagggcagg
     2761 ctttggagga gctgttcttc cagtatggac ggtatttatt gattagttcg tccagagact
     2821 gcccagatgc tctaccagct aacctacagg gagtctggaa ttcggactat cacttaaatg
     2881 tcaatctgca gatgaattat tggccagcct atgttaccaa tctcctagag gcggtctttc
     2941 cggtcatcaa ctatgtagat gatttgcgtg tctatggtcg tctagcggct gtaaagtatg
     3001 caggaatcgt ctctcaaaaa ggtgaggaaa atggttggtt ggttcatact caagcgactc
     3061 cctttggttg gacggcacct ggttgggatt actattgggg ttggtcacca gctgccaatg
     3121 cgtggatgat gcaaaccgtt tatgaagcct attcatttta tagggaccaa gactatctca
     3181 gggagaaaat ttatcccatg ttgagggaaa cggttcgttt ttggaatgcc tttttacata
     3241 aggatcagca ggcgcagcgt tgggtgtctt ctccgtctta ttctccagaa catgggccga
     3301 tttcgattgg caatacctat gaccaatctc tgatttggca gttatttcat gattttattc
     3361 aggctgctca ggaattggga ctggatgagg acttgttgac tgaggttaag gagaagtctg
     3421 atttactaaa tcctttgcaa atcactcaat ctggtcgaat cagggagtgg tatgaggagg
     3481 aagagcagta ttttcaaaat gagaaagtgg aggcccagca tcggcacgct tcccatctag
     3541 tgggactcta tcctggcaat ctctttagct acaagggaca agagtatatt gaagcggcgc
     3601 gtgctagcct caatgatcgt ggagatggcg gcacaggctg gtccgaggct aataagatca
     3661 atctctgggc gcgtttggga gatggcaatc gagcccataa attattggca gagcagttaa
     3721 agacatccac cttgcaaaat ctttggtgta gccatcctcc ttttcagata gatggtaatt
     3781 ttggtgctac tagtggcatg gcagaaatgt tactccagtc tcatgcagct tatctggtac
     3841 ctctagctgc cctacctgat gcttggtcaa caggttctgt ttcaggctta atggcacgtg
     3901 gacattttga agtgagcatg agctgggaag ataaaaaact cttacagttg accattttat
     3961 caaggagtgg aggagatttg cgagtttctt atccagatat tgagaagagt gtgattaaaa
     4021 tgaatcaaga aaaaataaaa gcgaaatgca tggggaaaga ttgtatttcg gtggcaacag
     4081 cagaaggtga tcttgttcaa ttttattttt aagaagatgt tataaggcag taatttgaaa
     4141 ctgcctttta ataaggattt aagaatataa gcagttttca actagttgaa aaaacgttat
     4201 aatgataata ggaagtaata ctcaatgaaa atcaaagagc acaaactagg aagctagccg
     4261 caggttgctc aaaacagtgt tttgaggttg cagatggaag ctgacgtggt ttgaagagag
     4321 attttcgagg agtataattt gtttgataga gggtgggtct gatggcttat attgagatga
     4381 aacactgtta caagcgttat caggttgggg acacggagat tgtggccaat cgtgatgtga
     4441 attttgagat tgaaaagggg gagctggtta ttatccttgg tgcttcaggt gcaggcaagt
     4501 caacagttct taaccttctt gggggaatgg ataccaatga tgaaggggaa atctggattg
     4561 atggtgttaa tattgcggat tatagttccc accagcgcac caattaccgt agaaatgatg
     4621 tggggtttgt ttttcagttt tataatctag tttctaatct gacagctaag gaaaatgtgg
     4681 aactggcttc tgaaattgtg acagatgcct tgaattctga tcaggtcttg acagatgtag
     4741 gtctggctca tcgtctcaat aactttccag cccagctttc tggaggggag caacagcgag
     4801 tctccattgc acgcgcggta gccaaaaatc ctaaaattct tctttgtgat gaacctactg
     4861 gagccttgga ttattagacg ggcaagcagg ttttgaaaat tctccaagac atgtctcgtc
     4921 aaaagggagc gacggtgatc atcgtgactc ataatggagc tttggcgccc attgctgatc
     4981 gcgtgattca tatgcacgat gccagtgtca aggatgtggt gctcaaccag catcctcagg
     5041 atattgacag tttggagtac tagcatgatc aagcgaaaaa cttattggaa ggacttaatt
     5101 cagtccttca caggctccaa ggggcgtttt ttatccatct tgatcctgat gatgttggga
     5161 tctctagcct tagtaggcct caaagtaacc agtcccaaca tggaggcgac agctaatgct
     5221 tatttaacaa ctgctcaaac cttggatttg gcagtcatgt ctaactatgg cttggatcaa
     5281 gcagaccaag aagaactaaa acagacggag ggcgcagagg tcgagtttgg ctatttgaca
     5341 gatgtgacta tggataatgg gcaggatgcc attcgactgt actccaaacc agagcgaatt
     5401 tcaacctttc agctaagaaa gggacgactt cctcagtcag acaaggaaat cgctttggcc
     5461 actcatttgc aaggccaata cagcgtggga caggagatta gttttaaaga aaaagaagag
     5521 ggtcattcct ctttaaaaga ccatacttat accattactg gttttgtgga ttcggctgaa
     5581 atcctctccc agcgagatat gggctacgca ggaagtggaa gtgggactct gacagcctat
     5641 ggggtgattt tacctagtca gtttgatcag aaagtctaca atatagctcg tttgaaatat
     5701 caagatttag caggtttaaa tgccttttca tcagcttatg aagaaaaatc caagcaacat
     5761 caggaagagc ttgaacaaat tttatcagat aatggcaagg tacgtctgca acttttgaaa
     5821 aaagaaggac aagagtctct agacaagggg caagagaccc ttgacaaggc tcagactaat
     5881 ttgcaggaag gcaagcgtcg tttagcagct gctcaagctc gtatacaggc tcaagaaagt
     5941 caactagcct tgtttcctca agttcagaga gagcaggcta gtgctcaact tacccaagcc
     6001 aagcaggaat tgggcaagga agaggccaaa ctaaagcaag ctgaacaaaa tctagcccaa
     6061 gaaaaggaaa aattagaaaa acatcagcaa gtcttggacg atttggcgga gccaaggtat
     6121 caggtctata atcgtcagac catgccaggt ggtcagggct atcttatgta tagcaatgct
     6181 tcatccagta ttcgagcagt gggcaatatc tttcctgtgg tactttatgc cgtagcagcc
     6241 atggtgacct ttacgaccat gactcgcttt gtagacgaag agcgaactca tgcagggatt
     6301 tttaaggcct tgggttaccg tagtaaggat attatcacca agtttctcct ttatggacta
     6361 gtagctggga ctgtcggaac ggctctaggt agtatacttg gtcattattt gctagccagt
     6421 gtaatttcaa gtgtcattac aaaaggcatg gtggtgggag aaacccagat tcagttctat
     6481 tggacctata gcttactagc tcttgtcttg agcttgttgg cgagtgtgtt accagcctat
     6541 atggtggctt ggagggaact tcatgccgaa gcagcccagc ttctacttcc taaacctcct
     6601 gtcaaaggag ctaaaatctt attggagcgt atcggtttta tctggcgtcg tctcagtttt
     6661 actcataagg taacagcccg caacatcttt cgttataagc agagaatgtt gatgacaatc
     6721 tttggtgtgg caggttctgt agctctgctc tttgcaggtt tgggaatcca atcttctgta
     6781 gcaggagttc cgtctaaaca gtttcaacaa atccaacagt atcagatgct tgtctctgaa
     6841 aatcctagtg cgaccaatca ggacaaggta gagctagcag aagtgttgaa agggcaggag
     6901 atactagcct accagaaaat ctattctaaa acgctagaca aggatttcaa aggcaaggct
     6961 ggtcttcaaa acattactct tatgatgata gagaaggaag atttgactcc ctttatccat
     7021 cttcaacatc atcagcagga gctgacatta aaagatggca tcgttattac agctaaactc
     7081 gcccagctgg caggtgtcaa ggttgggcag actttagaaa ttgaaggtaa ggaactaaag
     7141 gtcgctgcta ttactgagaa ctacgttggt cactttattt atatgagtca ggctagctat
     7201 gagcaacttt acggacagct accccaagcc aacacttatc tggtctcatt aagggatacc
     7261 agtgcaacta gtatcgaaag tcaggcgggc ttgcttatga atcaatctgc ggtgtccagc
     7321 gttgtccaaa atgcttcagc cattcgactc ttcgactcta tcgctagctc actcaatcag
     7381 accatgacca tcttggtcat cgtatcggtt ctattagcta ttgtcatcct ttacaatctg
     7441 accaatatca acgtagctga gagaatccgt gaactctcca ctatcaaggt tctcggtttt
     7501 cataataatg aagtcaccct ctacatttac cgtgagacga ttgtgctgtc ccttgtggga
     7561 atcgtacttg gtctggtagc tggtttctat ttacaccaat ttttgattca aatgatttcg
     7621 cctgcgacta ttctctttta tccgcaggta ggctgggaag tctatgtaat cccagtggca
     7681 gcagtaagca tcattttgac cttgcttggt ttcttcgtca attattatct gagaaaggtt
     7741 gacatgttag aagccctgaa atctgtagag taaggtagtt atttttagct gattgaactt
     7801 ctatttacta atattcaaaa atcctccgtt tcaaagagca gggaactctt tgtgacagag
     7861 gattttttct atagggcttt agcagctgca attgcggctt cgaagtttgg ctcagaattg
     7921 atattatcca cgtattcaac gtagcgaatc gtattgtcag tatcgaggac aaagactgcg
     7981 cgtgctaata ggtgccattc gttgatcaag agggcataat cgtgcccgaa agaatggtca
     8041 aagtagtctg aaagcataat ggcattgtca aggccttcag caccgcacca acgtttttga
     8101 gcaaaaggta ggtccattga aacagtcaat acgaccgtgt tgtccagtcc agccaattct
     8161 tcattaaaac gacgtgtttg agttgagcag atgcctgtat cgatagaagg aacgacactc
     8221 aagacttttt tcttgccatc aaaatcagcc agagattttt tagaaagatc tgttgtagta
     8281 agagaaaaat caagcgcctt gtcgccgact tgtagttgtt tacctgtaaa gctcacagga
     8341 tttccgagaa aagttaccat aggatactcc aatctttttt cttccattgt atctgaaaca
     8401 gtcagaattt tccaatgatt tgaccggaaa tgtgggcata gaaaaaacgc cagctcatat
     8461 gagaatgacg tttttcagag gcttattttg ccaatccttc agcaatcttg tcaaggttgt
     8521 atttcatcat gctgtagtag ctgtcgcctt ctttaccttg ttctgcgata gagtcagtaa
     8581 agatttgtgc gtagattggg atgtttgtgt cttgagaaac ggttttcatt ggacggtcat
     8641 ccacacttga ttctacaaag agtgatggaa cttttgtttg gcgaagtttt tcaaccaagg
     8701 tcttgatttg ttcaggagtt ccttcttctt cagtattgat ttcccagatg taggcacttg
     8761 gaacaccata ggctttagag aagtatttga atgctccttc gctggttaca atgagtttct
     8821 tttcagcagg gatcttatta aatttatcct tactttcttt atcaagtttg tctaacttat
     8881 cagtatattc tttgagattt ttttcataga attctttatt gttagggtct ttggcgctca
     8941 attgtttggc gatattttta gcaaaaataa taccgttttc aaggttaagc caagcgtgtg
     9001 ggtcttcttt tcctttttca ttttgacctt caaggtagat aacatcaacg ccgtcgctga
     9061 ctgcgaagta gtctttgttt tcagttttct tggcattttc taccaatttt gtaaaccaag
     9121 cattgccacc tgtttcaagg ttgataccgt tatagaaaat caaatcagcc tcagaagttt
     9181 tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc ttgcccaatc ggaacgatac
     9241 tatgaaggtc aattttgtca ccagcaatat ttttagtaat atcagcgatg attgagtttg
     9301 tagcaacaac ttttagtttt tgaccagaag ttgtatcttt ttttccgcta gcacatgcta
     9361 caagaatgat tgcagaaaga aagagaacga gtaatgtacc taattttttc attagatcct
     9421 ccaatttatt agggctttgc cccttatttt aacaaatgtt tatttttcag tttcaaatat
     9481 cgttgtttgg gagcgataaa gaagctaatg agaaagaaac tagcagctgt aagcacgata
     9541 ctagaacctg ccgcaacatt aaaactatag ccaataaaga gtcccaaaac tgaagcagta
     9601 gctccgaagg ttgaggaaag gaaaatcatg cttttcagac tattagcata cagataagca
     9661 gttgcagctg gggtaatcag catggctaca atcaggatag ttccgacact ttgcatggct
     9721 gtcacagaca cgagagtcag gagtaccatg agaaggtagt gatagaaatt gacaggcatt
     9781 cccatggctt tagccaagag ttcatcaaag gaagttatca agagttgctt gaagaaaatc
     9841 cagattaaca agaggatggc tgcccccaca cccatagtaa taaacatatc cgtatcttgg
     9901 acggccagga tattaccaaa aaggatatgg aaaaggtcag ttgaactttt agcgacacca
     9961 atcaagatga taccgagggc taagaaagaa gaaaaggtaa tgccgatggc ggtatcgctt
    10021 ttgataatcg agtttccttt gatgtaggta atgatgatgg cagctagcaa tccaaagaca
    10081 atggctccga taaagaagtc aaggcccaag atgaaggaga gggctacacc tggtaagaca
    10141 gcatgtgaaa tggcatctcc catgagtgac atcccacgta gaataatgaa acatcccacg
    10201 gctccagcta caatcccgac gacaatagct gttatcaagg cattttgtag gaaatggaat
    10261 ttttgcaatc catcgataaa ttctgcaatc ataggtcacc tccattgaaa aagagttgat
    10321 taccgtaagc ttcttttaga ttggtttcgg taaaagtttc ttttgttgga ccaaaggcaa
    10381 tcacttctcg attgacaagt aagacttgat cgaagtagtg gggaatcttg ctgaggtcgt
    10441 ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc tctcagcgta ttcatgatga
    10501 tttcctcact gacagagtca atcccagcaa agggttcatc caagaggata tagtcggctt
    10561 cctgcaccaa acatctggca atcaagaccc gctggaattg acctccagac agttgactaa
    10621 tttgacgttc agcgtagtca gctaggccga cgatttcaag ggcctcttgc actttcttcc
    10681 aatgtttagc ctttaaactt cgaaagagag gaatagaggg aaatagtcct aacgagacgc
    10741 attccttgac cttgatggga aagttgtagt cgatattgat tttttgttcg acataggcaa
    10801 ttcggtgtaa ggatttttta acttccttgt catcgagaaa tgcctgacct tgatgtggga
    10861 taattcccag catacctttt aatagtgttg atttcccagc gccgtttgga ccaatgatgc
    10921 cggtaattgt tggtccatgg agcactagtg aaatatcctt aagtgccaac gtttctttgt
    10981 aggagacact gaggttttcg atacgtatca taaacttgta ttcctcctgt ctcttaatat
    11041 acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa attaaaataa taactgaaaa
    11101 atagattcta aagataactt tcaggataaa tttctaaatt ataaaacgca tagtatcaag
    11161 tgtaaaaaac ttggaattat gcgttttatc atggaaagat tttttataat agctaaaaaa
    11221 taatagttaa atggttatta attgcattcc ctagtgattt ttgttaagat aaatgcaaat
    11281 acaaatgaaa gcgagaacaa gatgacacgt tatcaagatg atttttatga tgctatcaat
    11341 ggagaatggc aacagacagc tgaaatccca gcagataagt ctcaaacagg aggttttgtt
    11401 gatttagacc aggaaattga agacctgatg ttggcgacaa cagacaagtg gttagcaggt
    11461 gaagaagtgc ctgaggatgc tatcttggaa aactttgtca aataccaccg cctagttcgt
    11521 gattttgaca agagagaagc tgacggtatc acacctgtct taccactcct taaagaattc
    11581 caagaattgg aaacttttgc ggattttaca gctaaactag cagagtttga gcttgcagga
    11641 aaaccaaact tccttccttt tggtgtatcg ccagacttta tggatgctag aatcaatgtt
    11701 ctatgggcta gcgctccaag cacaatcttg ccagatacga cctactatgc agaagaacat
    11761 cctcagcgcg aagaactctt gactctttgg aaagaaagca gcgcaaatct cctcaaggct
    11821 tatgatttct ctgatgaaga aattgaagac ttgctagaaa aaagacttga attggaccgc
    11881 cgagttgcgg cagtggtgct ctctaatgaa gaaagttcag aatatgctaa actctatcat
    11941 ccatattctt acgaagattt caagaaattc gcgcctgccc tacctttgga tgacttcttc
    12001 aaagcagtta ttgggcaatt accagacaag gttattgtag acgaggaacg tttctggcaa
    12061 gcagcagagc aattctacag tgaggaatcc tggtctctcc ttaaagcaac cttgattttg
    12121 agtgttgtca atctttcaac cagctattta acagaggata tccgtgtttt gtctggtgcc
    12181 tacagccgtg ccctttctgg agttccagag gcaaaagata aggtcaaagc agcttatcat
    12241 ctagcacaag aacctttcaa gcaagccctg gggctttggt acgcccgtga gaagttctct
    12301 ccagaagcca aggcggatgt ggagaaaaaa gtggcaacca tgattgatgt ctataaggag
    12361 cgtctgctta agaatgactg gctcactcca gaaacctgta aacaggctat cgtgaagctc
    12421 aatgtgatca aaccttatat tggctatcca gaagaattgc ctgcacgtta caaggataag
    12481 gtagtgaatg aaactgccag tctttttgag aatgctctag cctttgcgcg tgtggaaatc
    12541 aagcacagtt ggagtaagtg gaaccagcct gtagactata aggaatgggg catgcctgct
    12601 catatggtca atgcctacta caatcctcag aagaacctga ttgtctttcc agcggccatt
    12661 ttacaggcgc ctttctatga cttgcatcag tcatcttctg ctaactacgg tggtattggg
    12721 gcagtgattg cccatgaaat ttcccacgcc tttgatacta acggggcttc ctttgacgaa
    12781 aatggtagcc tcaaggattg gtggacagag agcgactatg ctgccttcaa ggagaaaaca
    12841 caaaaagtca ttgaccaatt tgatggacag gattcttatg gagcaaccat taacggtaaa
    12901 ttgactgtat cagaaaacgt ggctgacttg ggaggaatcg cagcagcgct tgaagcagct
    12961 aagagagaag cagacttctc agcagaagag ttcttctaca acttcggtcg catctggcgc
    13021 atgaaaggtc gaccagaatt tatgaaactt ttggctagcg tcgatgtgca cgcaccagcc
    13081 aaactccgtg tcaatgtgca agtaccaaac ttcgacgatt tctttacaac ctatgatgtc
    13141 aaagaaggag acggaatgtg gcgttcacca gaggagcgcg tgattatttg gtaatacaaa
    13201 aaatctagtc atcagaaaaa aggcatccaa tcatgtgtga aaaccttgat aggatgcctt
    13261 ttgtaataga aagatttgct ggatagttta cttaaatttc gattgtcatc atcttgctag
    13321 aaaaagggat tgaaggcctt ttcgtgagcg atagtagtag ctggaccatg tcctggatag
    13381 acatcgtagt ttggtagggt gaagagttgg gtctggatac tatgaaggag ttgctccatg
    13441 ctaccagtcg gaaggtcggt ccgtccgata gtttcgcgga atagagcatc tcccgtcaag
    13501 actaggtgag catcaggaaa gactagggaa acaccaccga tagagtgccc tggggtcggt
    13561 agaaccttaa aacgaaattc ctctagttgg tattcttcgt gaaagacaaa ggtgtgttct
    13621 gcaggttttg tgaccacatc tgccatatca tcgtggcgag ggagaccgga gagattatcg
    13681 acaggagtgt agagccagct ggcttcgctc tctgcgatat agacaggagg attgccaaac
    13741 gtctcgcgaa ccaagtccag actcatgata tggtcataat gggcgtgggt caagagaata
    13801 gcacagatcg gtttgttgat cttctcgatt gtctgacgaa tggcttccca atgactacca
    13861 ggatcgacga cgatgaggtg cttttcgcct tctagataat aggtattttc ataggcaaca
    13921 ggattcacgg ttttatggat tttcatacta gctccaatct caaagaatgt actaatactc
    13981 ttcgaaaatc tcttcaaacc gcgtcaactt tatctgcaac ctcaaagcag tgctttgaac
    14041 agcctgcggc tagctttcta gtttgctctt tgattttcat tgagtataaa ttaagtatag
    14101 cacagttagg gagaataggt aaggatttaa aatgaactta aaaaccagca taactagatg
    14161 aagacaggtt ggttatcaat ttatcaatat tggaaaggtg tcaattgccc ttcataggtt
    14221 gcataaactc cgtcattagc ttgtttgata gacgtgatgt ttagagtgct accgtagttg
    14281 gcttctcatt tgtcgctata ttcactataa gttatcctac tgggcaggtt ctggtcgtta
    14341 gcatagtatt gaacgagttt tttatagctt tgctggctga ataggaacag gcagcaaact
    14401 cctaacatta ggagagcaaa gatagaaata gcagtttttt cataggtatt ctccttataa
    14461 actaaaggat agaagaaagt atcttgagga tttttatact cttcaaaaat ctcttcaaac
    14521 cgcgtcaacg tcgccttgcc gtatatatgt gactgacttt gtcagtctta tctacaacct
    14581 caaagcagtg ctttgaacag cttgcggcta gtttcctagt ttgctcttgg attttcattg
    14641 agtattaatt ccaaaatcca gtcaaggtac cagtgtatgt gatacgagtt ccaccgttta
    14701 tctttcgtat cttcaatgga attggggaag tcagaaagtc tttgggttac aatatggttt
    14761 ccgttcttct atctcttctt ctatcaccta agtttaaaat ctaacttcgt agtatattat
    14821 gggccaataa ttcagaatta tcaagcaatt ttgaattatt tttttcttat tctgttccaa
    14881 tctagttttc atattcttaa aaaagtgata aaatggtagg aagaattgga gagtagagat
    14941 gccgaaagaa gtgaatttaa caggcgaaga agttgtcgct ttaaccaaag aatatttaac
    15001 ggaagaggat gttcattttg tccataaggc cttgatctat gctgttgaat gccacagtgg
    15061 tcaatatcgc aaatcaggcg agccttatat cattcaccct atccaagtgg caggtatttt
    15121 agctaagcta aagctggatg ctgtaacagt agcttgtgga ttcttgcatg atgtggtgga
    15181 agatacagat gcgaccttgg acgatttgga aagagagttt ggtcctgatg tgcgggtgat
    15241 tgttgacgga gttaccaagc ttggcaaggt cgagtacaaa tcgatcgagg agcaattagc
    15301 ggaaaatcat cgcaagatgc tcatggccat gtctgaggac atccgcgtta ttttggtcaa
    15361 actgtctgac cgcttgcaca atatgcggac cctgaaacat cttcgaaaag acaagcagga
    15421 gcgtatttcc aaagaaacca tggaaatcta tgccccactt gcccatcgtt tggggatttc
    15481 cagtgtcaaa tgggaattag aagacttgtc tttccgttat ctcaatccaa cggagtttta
    15541 caagattacc catatgatga aggaaaagcg cagggagcgt gaggccttgg tggatgaggt
    15601 agtcacaaaa ttagaggagt atacgacaga acgtcacttg aaagggaaga tttatggtcg
    15661 tcccaagcat atttactcaa ttttccgcaa aatgcaggac aagagaaaac ggtttgagga
    15721 aatctatgat ctgattgcta ttcgttgtat tttagatacc caaagtgatg tttatgccat
    15781 gcttggttac gtgcatgaat tttggaaacc gatgccaggt cgcttcaaag actatattgc
    15841 caaccgcaag gccaatggtt atcagtctat ccatacgact gtttatggac caaaagggcc
    15901 gattgaattc cagattcgaa ccaaggaaat gcacgaggtg gctgagtacg gggttgcggc
    15961 tcactgggct tataagaaag gtataaaggg gcaagttaac agcaaggaat cagctattgg
    16021 aatgaactgg atcaaggaga tgatggagct ccaagaccag gctgatgatg ctaaggaatt
    16081 tgtggactct gttaaggaaa actatctggc tgaggagatt tacgttttta ccccagatgg
    16141 agctgtccgt tcccttccca aagattcagg accgattgat tttgcctacg aaatccatac
    16201 caaggtcggt gaaaaagcaa ctggtgccaa ggtcaatggc cgcatggttc cactgacaac
    16261 caagttaaag acaggggatc aggttgaaat tatcgccaac ccgaactcct ttggacctag
    16321 ccgtgactgg ctcaatatgg tcaagactag caaggcgcgc aataagattc gccagttctt
    16381 taaaaaccaa gataaggaat tgtctgtcaa caagggtcgt gagatgctga tggctcagtt
    16441 ccaagaaaat ggctatgtgg caaataaatt tatggacaag cgccacatgg atcaagttct
    16501 gcaaaagacc agttacaaga cagaagactc cctctttgcg gccattggtt ttggggaaat
    16561 cggtgcgatt accgtcttta accgtctgac tgaaaaggaa cgccgtgagg aagagcgtgc
    16621 caaggccaag gctgaggcag aggagcttgt caaaggtggc gaggtcaagg ttgaaaataa
    16681 agaaactctc aaggtcaagc atgagggggg agtggttatt gaaggtgctt ctggtctcct
    16741 agtgcggatt gctaagtgtt gtaaccccgt gcctggtgac gatattgttg gctacattac
    16801 caagggtcgt ggtgtggcta ttcaccgtgt ggactgtatg aacctgcgtg cccaagaaaa
    16861 ctacgagcaa cgtctccttg atgtggaatg ggaagaccag tactctagct caaataagga
    16921 gtatatggcc catatcgata tctacggtct caaccgtaca ggactgttga acgatgtact
    16981 gcaagttctt tcaaatacaa ccaagaatat ttcaacggtc aatgcccaac caaccaagga
    17041 tatgaagttt gctaatatcc atgtgtcctt cggtattgcc aacctctcta cactgaccac
    17101 ggttgtcgat aaaattaaga gtgtgccaga agtttactct gtcaaacgga ccaacggcta
    17161 gttgtcctag ctcttactag aaaggctatt atgaaaatca ttatccaacg ggttaaaaaa
    17221 gcccaagtga gtatagaagg ccagattcag ggaaaaatca atcagggact tttattgcta
    17281 gttggtgttg gaccagagga ccaagaggaa gatttggact atgctgtgag aaaactggtc
    17341 aatatgcgga ttttttcaga cgcagaaggc aagatgaacc tgtctgtcaa agatattgaa
    17401 ggagaaatcc tctctatttc tcagtttacc ctctttgcgg atactaagag aggcaatcgt
    17461 ccagccttta caggggcagc taaacctgat atggcatcag acttctatga tgctttcaat
    17521 caaaaattag cgcaagaagt gcccgttcag acagatatct ttggagcaga tatgcaggtt
    17581 gagctggtta ataacggacc cgttaccatt atcctagata ctaaaaagag ataaaaaaga
    17641 ccaagcccag tcggcttggt ctttctcatc tatcataaaa tactccaaaa agaaatcggt
    17701 tcttgatatg cttgggggaa gtctcttttc aggctttggc agatgcgata ggaagggatg
    17761 agatgtccta ggatgaggag agttccctga aggaaaagtg gaatatcact taaagacacc
    17821 aaggagagaa tcatcaggct atcccatagg agaatttgta aagcaaaacg ccagaatctc
    17881 ggtctaatct gggaatagag ctgactaaag tggtcacaaa gttggtttga aagataggtc
    17941 aatagcacag gatgaaagaa aatgagccaa ccgctataaa tcaaaatcca gtcccaagta
    18001 agcccccctt tttaaatgtc aaaaatgcca gcatgattcc atcaatgaca aggagttgaa
    18061 tggttttgat gaagatgatt tttttcatgc taaaacctcc ttttccttag agacactccc
    18121 ctacaggtat tttagaatgt atattgctta gatattcctt attttattag ctttttaagg
    18181 tattaaagta gttatataag tttatgaagg gtatcctatc ctgcacggct aattttgagt
    18241 tcgaaactga ggagtttata actcaaaatt tcgattaaat cagcatttaa cgatagtatg
    18301 tttgtcataa aatgataaat ttttagattg tggaaatttt ttttaactta attcttgatt
    18361 ttttttctaa atgtgatata cttaatttac gggataaaaa accaccttag aaaggtgtga
    18421 taatgtgcaa aaagagaatg ttagaatgcg tttagcgtct gaatccagac agatattaga
    18481 taagcttaaa gatgatgttt acaataaatt aggctatgaa gtaagctatt ccagtattgt
    18541 tagtcaagct gttagagaat acgttcctaa aaaagaacgt atagattgga taaaattaaa
    18601 agagacagct ataccctttt catcgttaaa acagagcaat aattgggagt accagacatc
    18661 acttatgtta gaggaagatg tattaatctt actgtctgaa ctacaaaatt ttttcttaga
    18721 tgtcttccaa gcgaaacgta tacatagggc attttgtgta cgcctatgct tgaaagcaca
    18781 atttttatta agtaataatg attcttaata ctatcaatat taatgcggag tgtaatgctc
    18841 cgctaaaata aaacctatta cgggataaaa acactacttt atttaggatg gaaagaggaa
    18901 cgaatgaaga tgaaagctag agattcacca ttgaattgat tttgttttta gaaaaaatca
    18961 ataattagaa aaaatacgag gtaaaaaaat gaatagagat gtattagagt ttctgagaac
    19021 agagaccgct gaaaaaatta gtctttatat aagcgaggct aatagattgg agggcgatgt
    19081 aactctactc gcacctagtt ctcaagattt ggaagatatt aaaaatgcta tgctttccaa
    19141 ttctaatctc ggattaaaag ttgcgagact ggatgtgatg aagaaaatag catatgcttc
    19201 tacaagaaat cattatttaa caggggctac gatttttggg gatatttcga aaggaacata
    19261 caattgtgat cctaaaagtt atgtctaata taagggaggt taatccctcc ccttgttttt
    19321 gaaaatcgaa aggaaaagta taaaaatgaa agttaatgaa tatttctgga gaacgagctg
    19381 tcttatacta aaaaagaaga aatcgataaa atagtatacg tgttagagat gtgtttgtcg
    19441 taaaagtctg gatagttttc tcctaggtag tcagctgtca tataataaaa agtagaatgg
    19501 caagcagtga taattggcat aagagatgaa aaacgctgag aactaatgaa ggctaagaga
    19561 acaaaaaata gaaaatcaat ggtgagaacc cctaaatagt agaagcgaat ctttttattg
    19621 atttgaggat aaatctcagc gaattgatgt ttgagtcgga cgaccaagag aaatagcagg
    19681 agtccttgag caaggaagaa aattaaacca gagagcaaga gccactcaaa agatgtctcg
    19741 agtctagtga taaaaaatgc aaatagtagc aaatcaataa ctgcaattgc tataaaatgg
    19801 attctataga gttttttcat gacaagacct tcctctttta tctaacttca ttctactcca
    19861 aaagaatggg agttacaact aaaatgataa aaatagcaga agggagattc tcttaagttg
    19921 gctagtattc tttatttgag tttccttcta tgtatctaac ttcttcatca ttccagacaa
    19981 ataaagctcc gattgcattg aggatataaa agatgtattt accgatattg gcgaagtttc
    20041 cttgaatacc agcttttgtc agctgaacga aattgtaaat caaccaaaag ccccactgag
    20101 ttgttagttt caatgcattc aaagcattgg caatgaggga cagtgcaaag gcaatagttg
    20161 ttacgtaggc aaggagattc atcttgccct catatccgat atagttggtc acaaaggcaa
    20221 agaggaaggc gatgatggaa atgatgatgg ccgccaattt tacctgtttt tggctcattt
    20281 ggttgggtct gccttcttgc gaagctttcc atttctttat agcaaaggta taaatgagga
    20341 aggtgacggg ataggtaatg atggccgcct tatttccaag gatataatca atagcaccgg
    20401 acaaaatggt attaacaata ccaaagtaat ttccccattt gcttaatttc cccgtgaaac
    20461 gagtggacaa catggaaatc ccaacgttgg ttacggaaat caatccaaag ggtacaagag
    20521 ctgtccatgg tccccagtct acaaatttat cgaggcgtga gttgaggtaa ccagatgcaa
    20581 tcgcaatcca acgaccaaag caaccccgaa gaggtcaaac tatttagatg tagcaaaaat
    20641 ttttagtgat tttttcatag gttaaactac ctttcttttt ttcaaatatt ctcccaccaa
    20701 atgaaagtaa aataaaatga tagaaataaa accctgaaaa taaaactttc agggttggta
    20761 ggggacttga gaaattagtt gattttctcg acatagagtt gttttgcaat gtccatattc
    20821 acttgtaaat cctcgtcgtt actgaggata gtgaaggtat tgctgaagcc atcaaactgc
    20881 ttgacttgga gctggtcacc gatgtgaagt gagtgcttgt ccagataatg gagaatgtca
    20941 aaattatcgt gcacccgagt caggcggtag gcgccagctt ccttgatatc agctagtggg
    21001 aggttattga tttcaacgag gagttctccc ttggcaggaa tagttcctcc gtgggggcag
    21061 gttttaggga aacctagcag tttatctagt ctttccacga acaggtcaga gacagtgtgt
    21121 tccaagacct cagcttcctc gtgaatctgg tcacttgtat agtctaaatg atgaactaga
    21181 aaaacttcaa tcaagcggtg cttacgatag agctcagaga ctagtttgag accgaggtca
    21241 gtcagtagat agccacattc cttgtccttt aggatgaggt tttcactttt cattcgtttg
    21301 atcatttcag ttacggcagg gggagagact tgcatgcgag ccgcaatttc cttgttggta
    21361 atcttatgca ggtctatgcc aatttcataa atacatttta gatagtcttc tttgtttggg
    21421 gtcattcgtt tcctcttgtc taatatcttt atctagtata tcaaaaaaag ctagatttct
    21481 ctagctgtga ctttttatgt tatacttatt gcaagagaaa aaacgaggag atggatatga
    21541 cgaaaatagc tcttctttca gatattcatg gaaataccac cgccttggag gctgttttgg
    21601 cagatgctcg gcagctagga gtggatgaat actggctttt gggagatatt ctcatgccag
    21661 ggacaggacg tagaaggatt ttggacttgt tggatcaact accgattacg gctagagttt
    21721 tgggaaactg ggaagacagt ctttggcatg gtgtccgtaa ggaattggat agtactcgcc
    21781 ccagtcaacg ctatctcttg cgccagtgcc agtatgtttt agaggaaatt tccctagaag
    21841 aaattgaagt gctccacaat caacctcttc aaattcatcg tcagtttggg gatttgacgg
    21901 tgggaattag tcatcatctg cctgataaga actgggggcg agagttgatt catactggca
    21961 aacaagagga ttttgaccgc ttggtgactc atcctccctg tgatattgct gtttatggac
    22021 atattcacca gcagttgctt cgttacggga ctggtgggca attgattgtc aatccgggtt
    22081 cgattggcca acctttcttt ttggacgctc agctacggaa ggacttgcgg gcccagtata
    22141 tgattttgga gtttgatgac aagggcctgg tagatatgaa cttccgacgg gtagactacg
    22201 atgtagcagc tgaattgcag ctggctaaag acctgagact tccctatttt gaggtttact
    22261 atgaaagtct ggtcaatggg atccaccata ctcatcatca ggaatttttg agagaattgg
    22321 ctcagaaaga gggttgcgat cgggagttag acgactggtt gaaaagtggt aacgattgac
    22381 attacaacta aaaagggtat aataatagaa aaagaagagt agaggcaggg tagcctcgta
    22441 aaaaggagaa gaggatgcaa attccaagta gatttaccat tgcgactcat atgctgataa
    22501 tcattgccct cgaggggaag gaaagcaagg tgaccagtga ttttctggct gctagtgtcg
    22561 gggtcaatcc tgtcattatc agaaagatct tgtcccagtt gaagaaggca gagctgattt
    22621 cagtagcgcg tggaacgggc ggaacagaga ttgtcaagga ccttaaggat attagtcttt
    22681 tagatgttta tcaggcggtc gaatgtcttg gtaagacagg tcaactcttc agtttccatg
    22741 acaatccgaa tccaaattgc cctgtaggag ctcatattca tgatgttttg gatcaaaaat
    22801 tggagagaat tcagttgact atggaggcag aacttggtca gaccagtcta gaaaaagtcg
    22861 tggccgatgc agagagtcag atgaaggatt aagagggata aatgccctct ttttttcttt
    22921 ggataattat gataaaatgt agctattaaa ggagtaagaa atgtatctcg ttataggaat
    22981 tgtattagcc tttatagtgt cattttggaa ggataataga agtttgtgga atccagtatt
    23041 atttttatta tccctcattt caagttattt ttatctatcc tacctttttt ataaaaatgg
    23101 atacgagaat gttcagttag atttttatgt atttgccttt gtcttacttc cttttttgct
    23161 ttttctaagt ggcatctttt taatctataa tggggtcatt ttgttgaagc gagagggacg
    23221 ttccaagccc cattatctct caatgctctt tgatttttat tgactattag attctattta
    23281 aaaagactta gaatgcacat gaaagtatgt attctaggtc ttttgttttg tatacattta
    23341 attttcaaga gtattatcta tttttccatg cctggtaacg agtatcaatc aataactggg
    23401 taaggcgacc caaggtttct ctttcttctg gagtgaggga ttgagcttgg acaaagagat
    23461 gatgaatgta ttcaagcgcc tttaaggaag tcaagtcatt gatagagcta ataccagcat
    23521 caagaatagt gccaaagaag aggtcgaagt agagctgaag ttctgcgtca gggactgtgg
    23581 ccacccattc tttaaaggtt gtgtctactt gctggctatc actgttggtc ttatccagtt
    23641 ggacgaagtg cttgtcctca atctgccaac taaaggtatc gtgctgggcg atgccaccca
    23701 gggcagtact ctgaacgatg atttggtgag caggaatttc cagcatcata ccgataatgg
    23761 aaccttgtgg aatgaatatc ttgcttctat ccattatcct ttgataaccc gcagtctgtg
    23821 tcaattcttg atggagacca ggtgcatcaa atgtataaac tgctgtgatc tgattttgca
    23881 aactttgctc aatttggcta gcagcataga tagcgagatt tcctcccttg gaatgcccag
    23941 ccagaataac cttttgctta ggatgatggg caaaaaagtt ctttaaatag cgaagggcgt
    24001 gcttttgagc aggaatttcc ttcatatagg tcaggtggaa atcttccttc cagccaatga
    24061 tactgtcatc tgtcccacga aagacaatca gataggtatc gaggctgaca cgataagtca
    24121 tagccgcaaa ttgcttttgc agttcagggt cgatgtcgtt gataaaatgg gagagtttgc
    24181 aatttttgaa gcgcttgtgt tgagccaatt catctaataa ttgaaggcga tttttgctag
    24241 taagcatggt gggctctctt ggaacctgag gtgctagatc taaaagacgt tgaggaagtg
    24301 tggagaccag attatcaaag gagaggtagg tgatttctat taaggttaga atgtctaact
    24361 cattcaaggg aaggtcgtaa taagaatcat atgcgacatc tttcagatag tcaaaaatat
    24421 tggccataag agctcctttc tttttattta tcttagcaaa tttccctcaa attagctagt
    24481 agcattgcct gtttgtactg gctaaaaaca ggctatttca aattcagttt cagaccatct
    24541 ggcatggaaa aatctgttat aataatagaa aaggagaagc gcatgcacaa gattttatta
    24601 atagaagatg atcaggtcat tcgtcaacag gtcgggaaaa tgctctctga atggggattt
    24661 gaagtggtcc tggtagaaga ctttatggaa gttttgagtc tatttgttca gtcggaacct
    24721 catctggtcc tcatggatat tggtttgccc ttgtttaatg gttatcactg gtgtcaggaa
    24781 atccgcaaga tttccaaggt acctatcatg tttctttctt cgagagacca ggctatggat
    24841 attgtcatgg caatcaatat gggggcggat gactttgtga ccaagccttt tgaccagcag
    24901 gttcttttag ctaaggttca gggcttgttg cgtcgttcct atgagtttgg gcgtgatgag
    24961 agtttgctgg aatatgctgg tgttatcctc aataccaaat ccatggattt acattatcaa
    25021 gggcaagtct tgaatttgac caagaatgaa ttccagattt tacgcgtgtt atttgagcat
    25081 gcaggcaaca tcgtagcacg tgacgacctg atgcgggaac tttggaacag tgactttttc
    25141 attgatgata ataccctctc tgtcaatgtg gctcgtttgc gtaaaaagtt ggaggagcag
    25201 ggattggtag gatttatcga gaccaagaaa ggaatagggt acggattgaa gcatgcttga
    25261 ttggaaacaa ttttttctag cctatctgcg ctcccgtagt cgtcttttta tctatctgct
    25321 ttctttggca tttcttgtct tactctttca gtttttattt gccagtctag gaatttactt
    25381 cctctacttt ttcttcttgt gttgctttgt aaccatctta tttttcactt gggacatatt
    25441 ggtggaaacg caggtctatc gccaggaact tctctatgga gagagggaag ccaagtctcc
    25501 tttggaaata gctttagcag aaaaattaga agcgcgtgag atggaactct atcagcagag
    25561 gtcaaaagca gaaagaaaac tgacggattt gctggattac tataccttgt gggtccatca
    25621 gataaagacc cccattgcag ccagtcaact cttagttgca gaagtggtcg accgccaact
    25681 gaagcagcag ctagaacagg aaattttcaa aatcgactcc tataccaacc tagttttaca
    25741 gtacctgcgt ttagaaagtt tccatgatga tttgctctta aagcaggttc aaattgagga
    25801 cttggtcaag gaaataattc gtaaatatgc tcttttcttt attcaaaaag gcttaaatgt
    25861 caatctacat gaccttgata aagaaatcgt gacggataaa aagtggctgc tagtggttat
    25921 tgagcaaatc atctcaaaca gtctcaagta caccaaggaa ggtggtctgg agatttatat
    25981 ggatgaccaa gagctttgta tcaaagatac gggaatcggg ataaaaaaca gtgatgtcct
    26041 ccgagtattt gaacgtggct tttcaggata caatggccgt ttgacccagc agtcctctgg
    26101 acttggcctt tatctatcta agaaaatttc tgaagaactg gggcaccaga ttcgtatcga
    26161 gtctgaggtc ggaaaaggaa cgacagtgcg gattcagttt gctcaagtga acttagtcct
    26221 tgagtaaaaa tagatgagaa aaagcctcca gattggaggc ttttatgcta gactcttatt
    26281 tctcaacgcg tgatttgttg gcgatatcag ctaggatagc ttgaacaaaa ttatcaactg
    26341 agacagtttg tgtttctttt tggccgtagc gacgaacgtt gactgtttcg tcttccattt
    26401 ctttgtctcc aacaattaat tggtaaggaa tcttgctggt ttgtgaagca cggatcttga
    26461 actgcatttt ttcattgcgc tcatctacgt ctgcacggac accgcggtca cggagtttct
    26521 tggccacttc ccaagcgtag tccacgtgtt tttcgttaga tactgggatg agggttactt
    26581 ggtgtggtgc cagccatgtt gggaaggccc ccttgtagtt ctcaatcaag atagctgtga
    26641 agcgttccat agttgagata accccacggt ggatcatgac tggacggtga tcttcgccat
    26701 cagctccgat gtatttgagg tcgaagcgtt ctggcaacaa gaaatcaagt tggatagtag
    26761 aaagggtttc ttcttttcca agggcagttt taatctggat atccaatttt ggtccgtaga
    26821 aggctgcttc accttcggct tcaaagtagt ccacgcccat ttcatcaaga gctgcacgaa
    26881 gcatggtttg ggcattttcc cacatctcat cgttatcaaa gtacttatga gtatcttgag
    26941 ggtcacgaag agagaggcgg aagcggtagt cagtcaagtt gaagtcttca taaacatcga
    27001 taatcaactg aagggcacgt tggaattctt cttggatttg ttctggagta acgaataggt
    27061 gaccgtcgtt gagtgacatt tcacgtacac gttgaaggcc agtgagggca ccagattttt
    27121 cgtaacggtg catcataccg atttcagcga tacggattgg caattcacgg taagagtgaa
    27181 catggtgttt gaaaacttgg atgtggtgcg gacagttcat tggacgaagg acaaattctt
    27241 ccccgtcacc catgtccatg gttgggaaca tgtcttcttg gtaatgatcc cagtgaccag
    27301 aagtcttgta aagctcaaca gaagcaagtg gtggagtgta gacgtgttgg tagccagaag
    27361 ccaactcttt gtttacgatg tagtgttcca attcacgacg gatagtcgca ccatttggca
    27421 accagaatgg caaaccttgt cccacttctt gtgaaatcat aaagaggtca agctctttac
    27481 caagtttacg gtggtcacgt tccttagctt cttcacgcat ttgaaggtag tttttcaagt
    27541 ctttcttgtc aaaccaagct gtaccgtaga tacgttgcat catagcgttg tcgctgtttc
    27601 cacgccagta cgcaccagct acatggagaa ggtggaagat ttggatacga cctgttgatg
    27661 gaacgtgagg tccacggcag aggtctacat attcaccctg acgatagata gtcaaaccgc
    27721 cttcgtcttc tgagtgttct tcaatcaatt ccaacttgta agggtcattt ttgaagattt
    27781 cacgtgcctc gtctttagtc acttcttcac gaatagatgg gaagttttct ttgacgattt
    27841 tttgcatttc ttcttcgata cgaggaaggt cttcgttaga gatttgacca gctgtgttgt
    27901 cagtatcgta gtagaaacca tcttcgatgg ctggaccaac tcccaagtga atgtctggga
    27961 aaagacgacg agctgcttgg gcgaacaagt gagctgctga gtgacgcaag attggaaggg
    28021 catcttcgtg atcaggtgtc acaatttcga tgcttccatc ttcagtgata gcgcgagtag
    28081 tgtcgatgag tttgccgttg aatttaccag ccaaggcttt tttagctagg gaattgctga
    28141 tagattgggc aatttcaaaa gttgttacgc cagattcgaa ttcacgaaca gcgccatctg
    28201 ggaaagtaat gttaatcatg atgttctcct tttttattat ttcatagttt ctaagcattg
    28261 attttttatc agcagactac ttccgagtcc taacgttcaa ttgcttttct ttatcagaga
    28321 ggaagttttc catttccgtc agaatgtcta tatccaaacg ttgagaaagt tctaatagcc
    28381 accagatatt ttctgaaagt ttttgttcca gtgtgtagga tgtttcatca tagtagcgtc
    28441 cttgctttgt catcaccagt cattggaaat ttccaatatc attagataaa gccaagaggt
    28501 cttcttctac cgtccacttg aaatcatgat gcttaacttc cagttcgtga taagcttgtc
    28561 ggattgccca actgcgctcc actaattctt gcaactccat aattagtatc ctaataaaaa
    28621 aatgcgacat cctcgaaagg acgtcgcaac gtggttccac cttcatttat gtacctcaaa
    28681 agagagggac acctctgatc ggctctaacg tggccaccgt tttatgtttt cataaaaaac
    28741 tcaagagtag tatcagttta gacttcctat gcgtttccag caaccacgca ctctctagta
    28801 gaaagggact aagtgacttg tctctatgga ttattatagc atgattgtga gatttttcaa
    28861 ggattttgtg aaagtttttt gtttttcatt ttttgtagaa tatgcttgat gctaatacca
    28921 gaatccatgc ccaccagagc aactgtttca tagattagca agtaaattag tataaactca
    28981 ccaaaggtta tgatagtaaa gtaaaagagc agtccgttac caactaacca tcaaagtgag
    29041 aaacggaaac ggtagctata gagtaacgat acaatcaaga ttaccatagg tgtaaagaag
    29101 agaagattgt ttatatagag tcccttgagt gtaagggggt cagaaacaac tagcaagaac
    29161 aactcataaa gtggccaata aaagaataat atgaccagat agtaggggag atgggaatag
    29221 aatctacgca tgataatcac ctcgttttaa aaaaacagac aacttagatc tcttgctatg
    29281 attattatag catgtttaaa acaagaaagt aaatttgttg acaaagaaga tttttctttt
    29341 cactagaatc atagttagtt ttcttggtag aaatattttg ataataatta agcaagacta
    29401 ttgatattga ttattaagag tgtcacaatt gttgtaacta aaatgattac aacaatagag
    29461 gtataaaaaa tgaaactagc agttattgca gcaaatggac aagcggggaa agcaattgtt
    29521 gaagaagcag tcaaacgagg acatgaagta acggctattg ttcgttctga aaacaaaagt
    29581 caagctgagt ctattattaa aaaagattta tttgagttga caaaagatga tcttactgga
    29641 tttgatgctg ttatttcagc gtttggtgct tatacaccag atacgctacc gctccacagc
    29701 aaatcaatcg aactatttaa tcagcttctt gctggaacac agacacgttt tctagttgta
    29761 gggggagcag gaagtcttta tatagatgaa acaaaaacga cacgactttt agatacgtct
    29821 gattttcctg aagaatttaa gtcactagca aaagatcaag cggatgaatt ggatcttctt
    29881 cgtacaaaaa ataatctcaa ctggactttt gtcagtcctg ctgtagattt tattcccgat
    29941 ggtgaaaaaa caggaaacta cattctagca ggagaaattt ttactactaa tgaaaaagga
    30001 ataagccaga ttagttatgc agattatgct attggattgg tagatgaact tgaaaaaggt
    30061 catcatataa aagaacatat ctctttactt gaaaaataat aactatgaga gtattaatgt
    30121 ttgacagaaa atttaaatct tggtaaaata gggattgtcg taaagacaaa taacttcttc
    30181 ttggttacag gcatgccaac ctgtcactcg gatgaagcca aataaaaagg agaaacatca
    30241 tggcaatctc aaaagagaaa aaaaatgaaa tcattgcaca atatgcacgt cacgaaggtg
    30301 atacaggttc agtagaggtt caagttgctg tccttacttg ggaaatcaac caccttaacg
    30361 aacacatcaa acaacacaaa aaagaccacg ctacttaccg tggattgatg aaaaaaatcg
    30421 gtcgccgtcg taacttgctt gcatacttgc gtaaaaacga cgttaaccgt taccgtgagt
    30481 tgatcaactc tctaggactt cgtcgctaat tcaagataca aatgctgtca aaagcacaaa
    30541 gcaaaaatag gaaaattgac gaagaaactt cagtttctag gagattttat cttttttgcc
    30601 aagtgcttag gccgtgttca attgagcata tcttgataat gaagctactc taaattgggt
    30661 agcttttttg cgttgtcacc ttacctcgat atactcaagt ataatctttg gttacggttc
    30721 ctagcactgt aaggtaaaat aaaccagctt ttctcccttc ggggagattt tttttgtttc
    30781 actgaaagtt tcgtacgatc ttcgcctcgt cgcctagtct ataaacgatt tatctagcaa
    30841 gaatcatgat gctaagggcg tcaaaaatcc gtatgaaaat agggaaatga cacagtgttc
    30901 gatgagcaca aggagtttca tctttttcac taggatttta gcccgggctc aaatcagctc
    30961 tctgacttca gagggctttt ttattgaggt tttatcggta acaattttgg agactacaaa
    31021 aacctcaaac ggtatcagct aattacacca taaaggtgcg cagttactcg gcttttcaag
    31081 ccgagtagct gtatgcaagc cccctagaag agcccactct ttacaaagta aagtatagta
    31141 tgttatactt tacatggaag tagtcactga attccagtta gaaattactt tgtaactacg
    31201 ttttgaggag gagtaaaatg ctttcctacg ttcgacatta cccactagcg atagctaaat
    31261 taatgtgtct gtgctctcct aaaatctgct gatttattac tgactaatac aggaggtttt
    31321 ttttatggga cagacaatca tatctgctat tggtgtttat atttccacca gtatcgatta
    31381 tttaattatt ttaattattt tatttgcaca gctatcacag aataaacaga aatggcatat
    31441 ttatgcgggg caatatctag gcacaggctt acttgtaggg gcgagtttag ttgctgctta
    31501 tgtcgttaat ttcgtgcctg aagaatggat ggttggattg cttggtttaa tccctatcta
    31561 tttagggatt cgctttgcaa ttgttggaga agatgcggaa gaagaagagg aagaaattat
    31621 tgaaagatta gaacaaagca aggcaaatca actgttttgg acagttacat tgctgacaat
    31681 tgcgtctggc ggagataatt taggtatcta tataccttat tttgcttcgt tagattggtc
    31741 acagaccctc gtggccttgc ttgtgtttgt aatcggcata attatctttt gcgagattag
    31801 tagggtgtta tcctctattc cgttaatatt cgagacaatt gaaaaatacg agcgaatcat
    31861 tgtgccctta gtattcattc tacttggact atacatcatg tatgaaaatg gcacgataga
    31921 gacttttctg atcgtgtaga tttttttgtt tcactaggat tttagcccga gctcaaatca
    31981 gctctctgat tttcagaggg ctttttcatt gagtataaat ggacaatctt aaagttactc
    32041 tattgtagag tggcttttga tttgattagg agattcttat aaatatagta aaatgaaata
    32101 agaataggac aaatcgatca ggacagtcaa atcgatttct aaaaatgttt tagaagtaga
    32161 ggtgtactat tctagttcca atctactata taaatgaaaa ccttagctgt ttggccaagg
    32221 ttcatttctt ctatgcaagt tcttctcttt tcttatctgg gttccagatg aagcttgcga
    32281 taaagctaaa gatgatggtt aggatagcga ggataatagc aaggatgagg gcagggatgc
    32341 ggataaacca ccagagtgga tttggttttt tatcattgtg ccattgtttc gtttcttcgt
    32401 ccagacgttg gaattctgtt tgaatacgat tggcgactgt ttcaatgcct tcatcattca
    32461 ttttcttgat atctgagata tcgattggat ttccaaagtt catatcgaca cgttcacggc
    32521 taatcaagcc cttcaaagtc atgggaccgg tgtaggtaac cggcatgata cggaccttgg
    32581 ccattttggc aatcagtgct gcgcccccct tgacatcgtt tgagtggcgg ctaccacttg
    32641 gaaacatgat gagagagcgg tcactttttt tgagaacgtt gataggatat ttgatggctg
    32701 aggcgctggg attttcacgg tcgatgggaa aggcgccaca catacgaatc caccaaccaa
    32761 agatacggtt ggtaaagagt tctttttttg ccataaagat gaactgtttt ggcttggtcg
    32821 caaaggccat ataaacagga tcccaccagg tacggtgagg cgcaactaaa atataatttt
    32881 catcttgatt aggaatttta tcagtattat gatagtgagc attgccattg atggaccata
    32941 agagcaatac aactaatcca cgcaaataag tataaaacat gcgatctcct tcgattgttt
    33001 tcttgttatt attatacctt atcaaaggag ggctggcaaa cttttccctt gactagatac
    33061 atatttagga tgaaattaga attctgttaa aaaaaatgat ataatagaat ttatggataa
    33121 aaataagatt atgggattaa cccaaagaga agtcaaggaa agacaggctg agggtttggt
    33181 caatgacttt accgcatcag ccagtaccag cacttggcaa atcgttaaac gaaatgtctt
    33241 tacccttttt aacgctttga acttagccat tgctttggct cttgcctttg tgcaggcttg
    33301 gagcaatctg gtcttctttg ctgttatctg ctttaacgct ttttctggga ttgtgaccga
    33361 gctacgagcc aaacacatgg tggacaagct caatctcatg accaaggaaa aggtcaaaac
    33421 catccgtgat ggtcaggaag ttgctcttaa tcctgaagaa ttagtgctag gagatgtcat
    33481 tcgtttgtct gcaggagagc agattcctag tgatgccttg gttttggaag gctttgcgga
    33541 agtcaatgaa gccatgttaa cgggagaaag tgatttggtg caaaaggaag ttgacggctt
    33601 acttttgtca ggaagtttcc tagccagtgg gtcagtttta tctcaagttc accatgtcgg
    33661 tgcagacaac tatgctgcca aactcatgct tgaggctaag accgttaaac ccatcaactc
    33721 ccgtatcatg aaatcgctgg acaagttggc tggttttact gggaagatta tcattccctt
    33781 tggtctggct ctcttgctgg aagccttgct tttaaaaggc ctgcctctca agtcatctgt
    33841 tgtaaactcg tcgacagctc ttttgggaat gttgcctaag ggaattgccc ttttgaccat
    33901 tacttcgctc ttgactgcag ttatcaagct aggtttgaaa aaggtcttgg tgcaggagat
    33961 gtactctgtt gagaccttgg cgcgcgtgga tatgctctgt ctggacaaga cgggtaccat
    34021 cacccaagga aagatgcagg tggaggctgt tcttccgttg acggaaacgt atggtgaaga
    34081 ggctattgcc agcatcttga ctagctacat ggcccatagt gaggataaga atccaactgc
    34141 ccaagccatt cgccagcgtt ttgtgggaga tgttgcttat cctatgattt ccaatcttcc
    34201 cttctcgagc gaccgcaagt ggggggctat ggagttagaa ggcttgggga cagttttctt
    34261 aggggcacct gagatgttgc tggattctga agtcccagaa gccagggagg ccttggagag
    34321 aggatcacgt gtcttggtct tagctctcag tcaggagaaa ttagaccatc acaaaccaca
    34381 gaaaccatct gatattcagg ctctagcctt gctggaaatc ttggacccca ttcgagaggg
    34441 agcagcagag acgctggact atctccgttc tcaggaggta ggactcaaga ttatctctgg
    34501 tgacaatcca gttacggtgt ccagcattgc ccagaaggct ggttttgcgg actatcacaa
    34561 ctatgtagat tgctcaaaaa tcaccgatga ggaattgatg gccatggcgg aggagacagc
    34621 tattttcgga cgtgtttccc ctcatcaaaa gaaactcatc atccaaacgt tgaaaaaagc
    34681 gggtcataca acggctatga caggggacgg ggttaatgat atcttggccc ttcgtgaggc
    34741 ggattgttct atcgtgatgg cggaggggga tccagcaacc cgtcagattg ccaatctggt
    34801 tctcttaaac tcagacttta atgatgttcc tgagattctc ttcgagggtc gtcgcgtggt
    34861 caataacatt gcccacatcg ccccgatttt cttgataaag accatctatt ccttcctgtt
    34921 agcagtcatc tgtattgcca gtgctttact aggtcggtca gagtggattt tgattttccc
    34981 cttcattccg atccagatta ccatgattga ccagtttgtg gaaggtttcc caccattcgt
    35041 tttgactttt gagcgaaata tcaaacctgt cgagccaaac ttcctcagaa gatccatgct
    35101 tcgtgcccta ccaagtgccc tcatggtcgt gtttagcgtt ctttttgtga aaatctttgg
    35161 aacgagtcaa ggttggtctg agttagaaat ctcaactctc ctctattatc tcttggggtc
    35221 aattggtttc ttatccgtat ttagagcctg catgccattc actctatggc gagtcctctt
    35281 gattgtctgg tcagtaggag gcttcctagc cacagctctc ttcccaagaa tccaaaaact
    35341 gcttgaaatt tcaaccttga cagaacaaac gttgcctgtt tatggtgtca tgatgttggt
    35401 ctttaccgtg attttcatcc tgactagtcg ctatcaagct agaaaataaa gaaagactgc
    35461 aatctgtgga ttgcagtttt tttaggtaca agattgccag ccgaaatgtg gtataataag
    35521 aggtaataga gtttggaaag tgagagaaaa tgatttcaaa gagattagaa ttggtagctt
    35581 cctttgtgtc acagggggct attttactag atgtgggaag tgaccatgct tatctgccta
    35641 tcgagttggt tgagagaggc caaatcaaaa gcgctattgc aggtgaggtg gtggaaggtc
    35701 cctatcagtc tgcggttaaa aatgttgagg ctcacggcct aaaggagaaa atccaagtcc
    35761 gtttagccaa tggcttggca gcttttgaag agactgacca agtgtctgtc attaccattg
    35821 ctggcatggg tggtcgtttg attgctagga ttttagaaga aggtttgggg aagttagcta
    35881 atgtagagcg tttgatcctc cagcccaata atcgtgaaga cgacttgcgt atctggctac
    35941 aggatcatgg attccagatt gtagcagaaa gcatcttaga agaagctgga aagttttatg
    36001 agattttggt ggtggaagca ggacaaatga agctatcagc cagtgatgtt cgctttggtc
    36061 ccttcttgtc caaagaagtc agtccagtat ttgtccaaaa atggcaaaaa gaagctgaga
    36121 agctagagtt cgccctcgga caaatcccag aaaaaaatct ggaagaacgt caagttctag
    36181 tagataagat tcaagctatc aaggaggtgc cccatgttag caagtgaagt gatccaagct
    36241 tatgaagcct tttgccctca ggaattttct atggagggag acagccgtgg tctgcaaatt
    36301 ggcactttag acaagggtat ccaaagggtc atggttgccc tcgatattcg tgaagagaca
    36361 gtggcagagg ccattgaaaa gggtgtggac ttgattatcg tcaagcacgc gcctatcttt
    36421 cgtcctatca aggacttgct tgccagccgt ccgcaaaatc agatttacat cgacctgatt
    36481 aagcatgata tcgcagttta tgtcagccat accaatattg atatcgttga aaatgggctc
    36541 aatgactggt tttgtcagat gctaggaatc gaggagacga cttatctgca ggaaacaggt
    36601 ccagaacgtg gaattggacg tattgggaat attcagcctc agacattttg ggaattggcc
    36661 caacaggtca agcaagtctt tgacctagat agccttcgaa tggtgcatta tcaagagaat
    36721 gatttgcaga agcctatttc aagagtagca atttgtggtg gaagcgggca gtctttctat
    36781 aaggatgctt tggcaaaggg ggcagatgtc tatatcactg gcgacatcta ctaccatact
    36841 gctcaggata tgctgtctga tggcttgtta gcattggatc caggtcacta tatcgaagtg
    36901 ctttttgtgg aaaaaatcgc agcactcctt actcaatgga aagagaaaaa aggctgggaa
    36961 ctagagattt tacctagtca agcatcgacc aatcctttcc gtcatatcta gttagaaagt
    37021 gaagacaatg aaaaaagttg ccattattgg agcaggaatt gtgggagcaa cagctgccta
    37081 ctacctctcg agagaaagtg acctagaggt gaccgttttt gaccatgagc aaggtcaagc
    37141 caccaaggcc gcagcaggaa ttatcagtcc ttggttttcc aaacgccgta ataaagcctg
    37201 gtataagatg gcgcgcttgg gggctgattt ttatgtggat ttattagctg atttagagaa
    37261 atcaggacaa gaaatcgact tttaccagcg ttcgggagtc tttctcttga aaaaggatga
    37321 atccaagttg gaagaactct atcaactggc tctccagcgc agagaagaat ctcccttgat
    37381 aggccagtta gccattctgg accaagcccc agctaatgaa ttattccctg gcttgcaggg
    37441 attcgacaac ttgctctatg cttctggtgg agcgagagta gatggtcacc tcttagtaac
    37501 tcgtttgctg gaagccagtc aggtcaagtt ggtcaaagaa aaagtgactc tgacactctt
    37561 atcatcaggc taccagattg gcgaagaggt gtttgatcag gttattttgg caacgggagc
    37621 ttggttgggg gacatgttag agcctttagg ttatgaagtg gatgtccgtc ctcaaaaagg
    37681 acaactacga gattatcagc ttgcccaaga catggaagat taccctgttg tcatgccaga
    37741 aggggagtgg gatttgattc cctttgcagg tgggaaatta tccttgggcg ctacccatga
    37801 aaatgacatg ggatttgatt tgacggtaga tgaacccttg ctccaacaaa tggaggaggc
    37861 ggccttgcct cattacccag ttttagctag agtgacttca agggctgagc gtgtgggaat
    37921 ccgtgcctat accagtgatt tctctccctt ctttgggcag gtgccagaat tggcaggtgt
    37981 ctatgcagcc agtggactag gttcatcagg cctcacaact ggtcctatca ttggttacca
    38041 tctagcccaa ctgatccaag acaaggagtt gaccttggac cctctaaatt acccaattga
    38101 aaactatgtc aaacgagtaa aaagcgaata atactcaatg aaaatcaaag aacaaactag
    38161 gaaactagcc gcgggttact caaagcactg
//
LOCUS       DAVMMR010000016        36665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMMR010000016 DAVMMR010000000
VERSION     DAVMMR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.016"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            89..1780
                     /locus_tag="VYS74_000772"
     CDS             89..1780
                     /locus_tag="VYS74_000772"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV3788837.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            complement(2095..2622)
                     /locus_tag="VYS74_000773"
                     /pseudo
     CDS             complement(2095..2622)
                     /locus_tag="VYS74_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2629..3105
                     /locus_tag="VYS74_000774"
     CDS             2629..3105
                     /locus_tag="VYS74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV3788838.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            3189..3896
                     /locus_tag="VYS74_000775"
     CDS             3189..3896
                     /locus_tag="VYS74_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788839.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            3897..5228
                     /locus_tag="VYS74_000776"
     CDS             3897..5228
                     /locus_tag="VYS74_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV3788840.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            5395..6270
                     /locus_tag="VYS74_000777"
     CDS             5395..6270
                     /locus_tag="VYS74_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV3788841.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            6388..7251
                     /gene="pstC"
                     /locus_tag="VYS74_000778"
     CDS             6388..7251
                     /gene="pstC"
                     /locus_tag="VYS74_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3788842.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            7244..8059
                     /gene="pstA"
                     /locus_tag="VYS74_000779"
     CDS             7244..8059
                     /gene="pstA"
                     /locus_tag="VYS74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3788843.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            8061..8813
                     /gene="pstB"
                     /locus_tag="VYS74_000780"
     CDS             8061..8813
                     /gene="pstB"
                     /locus_tag="VYS74_000780"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3788844.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            8828..9478
                     /gene="phoU"
                     /locus_tag="VYS74_000781"
     CDS             8828..9478
                     /gene="phoU"
                     /locus_tag="VYS74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3788845.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            9546..9962
                     /locus_tag="VYS74_000782"
     CDS             9546..9962
                     /locus_tag="VYS74_000782"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788846.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(10085..10525)
                     /locus_tag="VYS74_000783"
     CDS             complement(10085..10525)
                     /locus_tag="VYS74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3788847.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            10737..11753
                     /locus_tag="VYS74_000784"
     CDS             10737..11753
                     /locus_tag="VYS74_000784"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3788848.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            11775..12674
                     /gene="galU"
                     /locus_tag="VYS74_000785"
     CDS             11775..12674
                     /gene="galU"
                     /locus_tag="VYS74_000785"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV3788849.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12746..13423)
                     /locus_tag="VYS74_000786"
     CDS             complement(12746..13423)
                     /locus_tag="VYS74_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV3788850.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13407..13946)
                     /locus_tag="VYS74_000787"
     CDS             complement(13407..13946)
                     /locus_tag="VYS74_000787"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV3788851.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            complement(13958..15088)
                     /locus_tag="VYS74_000788"
     CDS             complement(13958..15088)
                     /locus_tag="VYS74_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV3788852.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIHFKGKDGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGIFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHSQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            complement(15156..15854)
                     /gene="dapD"
                     /locus_tag="VYS74_000789"
     CDS             complement(15156..15854)
                     /gene="dapD"
                     /locus_tag="VYS74_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV3788853.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVSENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            complement(16084..16995)
                     /locus_tag="VYS74_000790"
     CDS             complement(16084..16995)
                     /locus_tag="VYS74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3788854.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            complement(17060..19525)
                     /locus_tag="VYS74_000791"
     CDS             complement(17060..19525)
                     /locus_tag="VYS74_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3788855.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLASSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGATATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19667..20923
                     /locus_tag="VYS74_000792"
     CDS             19667..20923
                     /locus_tag="VYS74_000792"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV3788856.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEESYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            complement(21001..23065)
                     /locus_tag="VYS74_000793"
                     /pseudo
     CDS             complement(21001..23065)
                     /locus_tag="VYS74_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(23067..23345)
                     /locus_tag="VYS74_000794"
     CDS             complement(23067..23345)
                     /locus_tag="VYS74_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788857.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            23497..24345
                     /locus_tag="VYS74_000795"
     CDS             23497..24345
                     /locus_tag="VYS74_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3788858.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            24968..25540
                     /locus_tag="VYS74_000796"
     CDS             24968..25540
                     /locus_tag="VYS74_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788859.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(26585..28843)
                     /gene="glgP"
                     /locus_tag="VYS74_000797"
     CDS             complement(26585..28843)
                     /gene="glgP"
                     /locus_tag="VYS74_000797"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV3788860.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(28869..30386)
                     /gene="malQ"
                     /locus_tag="VYS74_000798"
     CDS             complement(28869..30386)
                     /gene="malQ"
                     /locus_tag="VYS74_000798"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV3788861.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            30936..32207
                     /locus_tag="VYS74_000799"
     CDS             30936..32207
                     /locus_tag="VYS74_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3788862.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            32318..33610
                     /locus_tag="VYS74_000800"
     CDS             32318..33610
                     /locus_tag="VYS74_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3788863.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            33612..34454
                     /locus_tag="VYS74_000801"
     CDS             33612..34454
                     /locus_tag="VYS74_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3788864.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            34715..35515
                     /locus_tag="VYS74_000802"
     CDS             34715..35515
                     /locus_tag="VYS74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV3788865.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            35525..36511
                     /locus_tag="VYS74_000803"
     CDS             35525..36511
                     /locus_tag="VYS74_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3788866.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
BASE COUNT        11108 a         7839 c         6940 g        10778 t
ORIGIN      
        1 taccaaaaaa acaggagatt tcaaaaattt tttcttcttt ctttaaaatt gtgataaaat
       61 agtagaaaag tccaaaaagg agctcttaat gaatacaaaa gaattgattg ctagcgaatt
      121 atctagcatc attgatagcc tggaccaaga ggctatttta aagttactgg aaacccctaa
      181 aaactcagaa atgggagaca ttgctttccc tgctttttct cttgccaaag tcgaacgtaa
      241 agcaccacaa atgattgcgg ctgaactggc tgaaaaaatg aacagccaag cctttgaaaa
      301 agttgtcgca acaggacctt acgttaactt tttccttgat aaatctgcca tttctgctca
      361 agtattgcaa gctgttacca ctgaaaaaga acactatgct gaccaaaata ttggcaaaca
      421 agaaaatgtt gttatcgaca tgtctagtcc gaatatcgct aaaccatttt ctattggcca
      481 cctgcgttca actgttatcg gagatagctt gtcacatatt ttccaaaaaa tcggttatca
      541 aacggtcaag gtcaaccatt tgggagactg gggtaaacaa tttgggatgt tgattgttgc
      601 ctacaaaaaa tggggcgacg aagaagctgt aaaagctcat ccaatcgatg aactccttaa
      661 actctatgtc cgcatcaacg ctgaagctga aaatgaccct agcttggatg aagaagcgcg
      721 cgaatggttc cgtaaacttg aaaatggaga tgaggaagct ctcgctcttt ggcaatggtt
      781 ccgcgatgaa agtttagtgg aatttaaccg cctttacaat gaattgaagg ttgaatttga
      841 cagctataac ggagaagcct tctacaatga taagatggat gcagttgtag acattctttc
      901 tgaaaaagga ctacttcttg aatcagaagg tgcccaagtt gtgaatcttg agaaatatgg
      961 aattgaacat ccagccctca tcaagaagtc tgatggtgca actctctata tcacacgtga
     1021 cttggctgca gccctctacc gtaaaaatga ataccaattt gctaaatcta tctacgtcgt
     1081 tggtcaagag caatctgccc actttaaaca actcaaagct gtcttgcaag agatgggcta
     1141 cgattggagt gacgacatta cccacgttcc ttttggtctg gttacaaaag aagggaagaa
     1201 gctctctact cgtaaaggga atgtcatctt gctagagcct actgttgcag aggctgttag
     1261 ccgtgccaag gtccaaatcg aggctaaaaa tcctgaacta gaaaacaaag accaagtagc
     1321 acatgctgtt ggggttggag ccattaaatt ctatgacctc aaaactgacc gtacaaatgg
     1381 atacgacttc gacctagaag ctatggtatc cttcgagggt gaaactggac cttacgttca
     1441 atatgcctac gctcgtatcc aatctatctt acgcaaagcc gatttcaaac cagaaacagc
     1501 tggcaactat agcttgaatg atactgaaag ctgggaaatc attaaactca ttcaagactt
     1561 cccacgtatt atcaaccgtg cggcagataa ctttgaacct tctatcattg ctaaatttgc
     1621 aattagcctg gctcaatcct ttaacaaata ctatgcacat acacgtatct tggatgaaag
     1681 cccagaacgc gacagccgtc tagccctcag ctacgcaacc gcagtcgttc tcaaagaagc
     1741 ccttcgcttg cttggagtag aagcgccaga gaagatgtaa atcgccaaag ttacttgatt
     1801 gcaaagcaat ctaagtaact aacgccaaat cctattcgga ctttggctag gcgcttcact
     1861 agttttagga cataaatatg atcgatttat gtcctaaaac gtcgtaatct taaagttatt
     1921 ggaactaaag ttcctaattg ttagacgcta gccgcttata tgcgaactag ctaactgctt
     1981 cactagtcat gacccctaaa tataatcgat ttagaggcca tgacgtcgta accagagaca
     2041 cttgcctaaa cacttcacta attcacctaa ccaaataata gacttcctgc aaaactagaa
     2101 tcctagttca tgattgataa taccagcaat caaattcatt cgtaatccga agcgtttacg
     2161 atgatttcga taggttgttg aaaatatttt aaacgttttt actttggcaa agatgttctc
     2221 aaccttgctt ctttccttag atagcgcatg gttataggct ttatcttcag ctgttagcgg
     2281 cttgagtttg ctggatttac gtggagtttg tgcttgagga tatatcttca tgagcccttg
     2341 ataatcactg tcagccaaga ttttaccagc ttgtccgata tttctgcaac tcattttgaa
     2401 caacttcata tcatggcaat agaaacaatt ctcccttgac ttgtgacaat cgcttgagcc
     2461 ttcatagcgt gaaatttctt tttaccagaa tcattcgcta attctttttt tagggcgatt
     2521 gatttgtact tccgtcgcat caatcattac cgtgtcctca gaactgagag gagttcttga
     2581 aatcgtaaaa ccactttgaa caagagttac ttcaacccat tggccccgat ggataaagaa
     2641 gacttgataa gaactctaga gttagctccc catgttgaag gtggctattt tagacaaaca
     2701 aaaaaagctt cagacactat aaaccttgct ggtaagggaa gggcgcttta taccagtatt
     2761 tatttcttat tagaagaaac gaatccttca cattttcacc gattgacagc agatgaaatc
     2821 tggtattttc atgcaggatc cccacttact gttcacatga ttacagcaga tggtcactac
     2881 gaagcagtca ctttaggcct ggacatatcc aaagggcaac aacttcacta ctgtgttcct
     2941 aagggcacta tttggggatc aacagttgat aaggattatg ctctggtttc ttgtcttgtt
     3001 gctcctggtt ttgaatttga ggattttgaa ttatttgaaa gggtagatct actggcgacc
     3061 tatccagagc acaaggagat gattgagcgt ttgaccagat actagtcttg ataagcaaat
     3121 ttaatctgat ttagcaatgt taggatttct gataattgtg ttataatgtt accaatagga
     3181 gataatagat gacaaaacaa gtcttattag tggatgatga agaacacatt ctgaaattgc
     3241 ttgactacca tttaagtaag gaaggctttt ctactcaatt ggtaacaaat ggacggaagg
     3301 ccttagcttt ggcagaaaca gaaccctttg attttatctt gcttgatatc atgttaccac
     3361 aattagatgg catggaagtt tgtaagcggc tgagagccaa aggcgtcaaa actccaatta
     3421 tgatggtttc tgcgaaaagt gatgaatttg ataaggtttt ggccttggaa ttaggggctg
     3481 atgactacct gaccaagcct tttagcccta gagaattgct ggcgcgtgtc aaggctgtcc
     3541 tcaggcgaac taaaggagaa caagaaggag atgattcaga taatatcgct gacgattctt
     3601 ggctatttgg gaccttgaaa gtataccctg agcgtcatga agtctacaag gcgaataagt
     3661 tactgagttt gaccccaaaa gaatttgaac tcttgctcta tcttatgaaa catcccaaca
     3721 tgacactgac tagagagcgt cttttggaac gtatctgggg gtatgacttt gggcaggaaa
     3781 cacgtttggt ggacgttcat attggtaagt tgagggaaaa aattgaagac aatcctaaag
     3841 cccctcaatt tattcgaacc attcggggtt atggttataa gttcaaggag ttatagatga
     3901 aacgctacct tcaattttgg ctagtcaatc taagtgttag cctcattctg attgcaggga
     3961 tggcattaac ttggatcagt aaaggcatcg gtctctttct tctagccctt tctttgggac
     4021 aaggtggcta ctggctcttt tgcctttgga aatgggaagt tgcttttgag accttgcacc
     4081 aacctctctt gaccagtagt gaatactttt tagaaaaagg gcaagaagac ttaaagtctt
     4141 tggctcagta tgtgtctggc ctaaaaacca aagtctccca acaggatcag caatacaagg
     4201 acctagccga aacaatggag gttcttctgt cgcacctaac catggggacg tttttagttt
     4261 ccgctcaagg tcagatgtta ttatccagtc gctctctgcc tcattatttt cccgatgtgg
     4321 acggcgacat aagttcactt gatgacctca aacggatgga tattcggaat ttagttcacc
     4381 aagcctttga tcaaaaaaca aggttaaaac aagaagtaag tgggtttcat gagggtgact
     4441 tgattttaga agtgacagca gttcccgttt ttagccccac ccaatctgtg gaagctgtgc
     4501 tcgtcttgct atatgattta acaacgatta gaacttatga aaagttaaat ttagcctttg
     4561 tctcaaatgc ctcccatgaa ttgaggacac cagttacttc aattaagggc tttgctgaaa
     4621 ccattaaggg gatgtcagct gaagaagaag cgctcaagga tgactttcta gacattattt
     4681 acaaagaaag tttgcgtctt gagcatattg ttgagcatct tcttacctta tctaaggctc
     4741 aacaaatgcc tatacaatgg acgacccttt ctttggctga atttgtacag gatttgactc
     4801 aaagcttgca acctcagctc aagaagaagg atttacagct aaaggtgcag gtgccagatg
     4861 atgtcaccct cgtatcagat agtcaattac tttcccaaat cttactcaat cttttatcca
     4921 atgccatccg ttacactgaa caagggggaa aaattgaggt caagacccaa aaggtgaacg
     4981 aaggcattaa gatttctgta tcagatacag ggattggtat tagtcaatta gagcaggatc
     5041 gtatttttga acgtttttac cgagttaata aaggtcgaag cagacaaact ggtggcactg
     5101 gtcttggcct tgccattgtc aaagaactca gtcaattatt aggtggccaa gtcacggtga
     5161 cgagtcagct tggtagaggc agttgcttca cgatttttct tcctaaccaa tctttcgcac
     5221 aggactaata atttttaaag ctatttttag gagcagccat ttttcaaaag tggctttttt
     5281 tgattcttta cacaatcttt acatgaatca gggataaacc tttacaaaga cttgttagac
     5341 tataaatgta gtaagcctac acaagaaaaa tacatagaga taaaggtgat tattatgaaa
     5401 ttcaaaaaaa tgcttactct tgcagccatt ggcttatcag gatttgggct tgttgcctgt
     5461 ggcaatcagt cagctgcttc caaacagtca gcttcaggaa cgattgaggt gatttcacga
     5521 gaaaatggct ctggaacacg gggtgccttc acagaaatca cagggattct caaaaaagac
     5581 ggtgataaaa aaattgacaa cactgccaaa acagctgtga ttcaaaatag tacagaaggt
     5641 gttctctcag cagttcaagg gaatgctaat gctatcggct acatctcctt gggatcttta
     5701 acgaaatctg tcaaggcttt agagattgat ggtgtcaagg ctagtcgaga cacagtttta
     5761 gatggtgaat accctcttca acgtcccttc aacattgttt ggtcttctaa tctttccaag
     5821 ctaggtcaag attttatcag ctttatccac tccaaacaag gtcaacaagt ggtcacagat
     5881 aataaattta ttgaagctaa aactgaaacc acagaatata caagccaaca cttatcaggc
     5941 aagttgtctg ttgtaggttc cacttcagta tcttctttaa tggaaaaatt agcagaagct
     6001 tataaaaaag aaaatccaga agttacgatt gatattacct ctaatgggtc ttcagcaggt
     6061 attaccgctg ttaaggagaa aaccgctgat attggtatgg tttctaggga attaactcct
     6121 gaagaaggta agagtctcac ccatgatgct attgctttag acggtattgc tgttgtggtc
     6181 aataatgaca ataaggcaag ccaagtcagt atggctgaac ttgcagacgt ttttagtggc
     6241 aaattaacca cctgggacaa gattaaataa aatgtttgct ccataaatct ctaaagagat
     6301 gcagacgttt catcgtacaa taagataaag aaggcaagta gggaggtgtc gtatctccct
     6361 tactttcttc actagaaagg acaagatgtg ataaaacaag ccttcaaaga agcagttttt
     6421 agggcaattt ttttcatgag tgcaacagta gctgttgtag ctattttgct aatctgtttc
     6481 tttattttta gtaatggctt acctttcatg gctaactacg gctttgcccg ttttttatta
     6541 ggcagtgatt ggtcgccaac gaacattccg gcaagctatg gtattttacc aatgatcgtt
     6601 ggttccttat taattacctt aggagcgatt gtgattgggg tgccaacagg catcttgaca
     6661 tcggtgttta tggtttatta ttgtccaaag cccgtctatg gcttcttaaa atcagctatc
     6721 aacttgatgg cggccattcc atctattgtt tatggttttt tcggcctaca attattggtg
     6781 ccttggatta gaagcttttt aggaaatggc atgagtgtcc taaccgcttc gttactatta
     6841 ggaataatga ttttgccaac cattatcagt ttgtcagaat ctgctatccg aacagttccc
     6901 aaaacgtatt attctggtag cttggctcta ggagctagtc atgaacggag tatttttagt
     6961 gtcatcttgc cagctgcgag atctggtatt ttatcagcag ttattttagg catcggtcgc
     7021 gcagtaggtg aaaccatggc agttattttg gtggcaggca accagccgat tattccaagt
     7081 ggactctttt caggaaccag aaccttaaca accaatattg ttctggaaat ggcttacgca
     7141 tcaggtcagc atagggaagc ccttattgca acctcagcag ttctcttttt ccttattctc
     7201 ttgattaatg cctactttgc ctacttgaaa ggaaaatcat cttatgagta aatacctgct
     7261 aaaacttctc gtttattgtt tttcagcttt aacctttggc tctctctttt taatcattgg
     7321 ttttatcctc atcaaaggtt tacctcatct aagtctatcc ctcttttctt ggacttatac
     7381 ttctgagaac atttccctta tgccagcgat tatttccacc gttattctgg tctttggtgc
     7441 tcttctttta gccttgccca tagggatttt tgctggtttt tatcttgtgg aatatacaaa
     7501 aaaagattcc ctttgtgtta aaatcatgcg attggcctca gataccttgt ctgggattcc
     7561 ttccattgtt tttggtctgt ttggcatgct cttctttgta gtcttcttag gttttcaata
     7621 ctctctgtta tcaggaatct taacctcagt catcatggtg ttgccagtca ttattcgctc
     7681 aacagaagaa gcccttttat ctgttagtga tagcatgcgt caagcaagtt atggacttgg
     7741 ggcaggtaag ttacggactg tttttagaat tgttctacca gttgccatgc caggtatttt
     7801 agctggagtg atactagcta ttggccgtat cgttggtgag acagctgccc tcatgtatac
     7861 attaggtacc tctaccaata cgccaagtag tctcatgtct tcaggtcgtt ctctagccct
     7921 gcatatgtat atgctgtcaa gtgaggggct acatgtcaat gaagcctatg ctaccggtgt
     7981 gattttgatt attactgttt taatgataaa tactctatca agcttattat ctcgaaaact
     8041 tgtgaaagga gcttcctagt atgggaacat tttcagtcag acacctagac ttattttacg
     8101 gggattttca agccttaaaa aatatttcga ttcaattacc agaaagacag attactgcct
     8161 tgataggccc atctggttgt ggcaaatcaa cttttctaaa aacccttaac cggatgaacg
     8221 atttggttcc ttcttgccat attgaaggcc aagtcctctt agatgagcaa gatatttata
     8281 gtagcaaatt caaccttaat cagctacgta agcgtgtagg gatggttttt caacagccta
     8341 atccctttgc catgtctatc tatgataacg tggcttatgg cccaaggaca catggtattc
     8401 gagacaaaaa acaattagat gccttagtgg agaaatcttt aaaaggggca gccatttggg
     8461 aagaagtcaa agatgatctt aaaaagagtg ccatgtcctt atctggcggt cagcagcaac
     8521 gcctttgcat tgcgcgagct ttagcagtag aacctgatat tctgttaatg gatgagccga
     8581 cttcagcctt agaccctatc tccactttaa aaattgaaga cctcattcag caactaaaaa
     8641 aggattatac gattatcatt gttacccata acatgcaaca agcttcacgt atttcagata
     8701 aaactgcttt tttcttaaca ggagaaattt gcgaatttgg agataccgtt gacgtgttta
     8761 ccaatccaaa agatcagcgc acagaagact atatttcagg acggttcgga taaggaagga
     8821 aaaacctatg agaaatcaat ttgacttaga attgcatgaa ttagaacaat cctttttagg
     8881 actagggcaa cttgtccttg aaacagcttc aaaagcctta ctggccttag cctccaaaga
     8941 caaggagatg gcagagctaa ttatcaataa ggatcatgct ataaaccaag gtcaaagcgc
     9001 tatcgaattg acctgtgccc gtttgttggc cttgcagcag ccacaagtgt ctgaccttcg
     9061 atttgtgatt agcatcatgt cttcttgttc agaccttgaa cgtatgggag accatatggc
     9121 aggcattgcc aaagctgttt tgcaactaaa agaaaatcaa ctagcccctg acgaagaaca
     9181 gttacaccaa atgggtaaat tatccctcag catgctagcc gatttattgg ttgcctttcc
     9241 tttgcaccaa gcctcaaaag ctattagtat tgctcaaaaa gatgaacaga ttgaccaata
     9301 ttattatgcc ttatcaaagg aaatcattgg acttatgaaa gaccaagaaa cctcaattcc
     9361 caatggaact caataccttt atatcatagg gcatctggaa cgcttcgctg attacattgc
     9421 taacatttgt gaacgcctag tctacctaga aacaggagaa ctagtggatt tgaattaatt
     9481 caactaatcc ttaaaagaga agagtacgat taagtgctct tttttatggt tgtaaaaaag
     9541 ttcatttgac caatttaagc agtgtagata gtgaggagct gtttcaattc tatcgtgaac
     9601 gagggaatgc tgaaaacttt atcaaagaaa ggaaagcagg attctttggg gataagacag
     9661 atagttcgac catgattaag aatgaagtac gtatgatgat gggctgtctg gcttataatc
     9721 tctacctctt tttaaagcag ctagctggtg atgaagtaaa ggccttgact atcaagcgtt
     9781 ttcgacgtct cttccttcat attgccggaa aatatgtctc tactgctaga cgacatattc
     9841 tcaaattctc aagtctatac gcctattcaa aacagtttca agccttattt gatacaatct
     9901 gccagataaa tctgatactc cctgttccat atagagctag agggcagggg aaaactgcct
     9961 aacagaataa gtcaccttat tttaaaaatc gagcatcaaa ccaagggagg agtctgccct
    10021 tttttaggaa aaaatcaaga caaatctcct caatcatgtc tcgaacatca gaaattaagc
    10081 aaaatcacca gaaggacagt atttcaacta gcttttctgg taatttttga actgtgtagt
    10141 tcgctagtgc tagatatgaa taatttggga tgataaatct ttcttcctca ggtagcctat
    10201 cataatactc ttcaaaaatc ttatcaaaaa cactctcttt cttttgggcg atagtttcat
    10261 cttcgtatgt aggagtcctc atcaagaaat acttcaattc taggtattcc ttatccaact
    10321 ctatataact tggcatcaac ttgtaatctt caacctccaa acgttcagca atatatttta
    10381 actttgttag tgttggtctg gattctccat tttcaattct aattaattga cggatactta
    10441 attcagactc atcaccacaa aattctgaac gactgatttt tttagccaaa cgtaatcttt
    10501 taattttttc gccaaactct cgcaacctac aagaacttcc tgagttgttt acctctatta
    10561 taagcatata ctgaatcaaa ctatctatca gatttcttct cactttaact aaagactaag
    10621 agtttatccc ttcatctcgg tctttgtgta tttttccacc ataccccagt aatgcaagtg
    10681 caaaatcccc tagaatatga tagaataaga gaaagaaatc tatcaaggag gaaatcatgg
    10741 aaaaacaaac cgtcgccgtc ttggggcctg gttcttgggg aaccgccctt tcacaagtct
    10801 taaatgacaa tggacacgag gtacgtattt ggggaaatct tcccgagcaa atcaatgaaa
    10861 ttaatacaca ccatactaat aagcactact ttaaagatgt cgttctagac gaaaatatca
    10921 ttgcctacac cgacttagca gaaacattga aaaatgtgga tgcgattttg tttgttgtcc
    10981 caacaaaagt gacacgactt gttgcccagc aagttgcaca aaccttggac cataaggtta
    11041 tcatcatgca cgcatcaaag ggattagaac ctgatagcca taaacgatta tcaaccattc
    11101 ttgaagaaga aattcctgaa catctccgta gtgatatcgt cgttgtttca gggcctagtc
    11161 atgcagaaga gaccattgtg cgtgacctaa ctttaataac tgctgcttct aaagatttac
    11221 aaacagctca atacgttcag gagctattta gtaatcacta cttccgactt tataccaata
    11281 cggatgttat cggggttgaa actgctggtg ctcttaaaaa tattattgct gtcggtgctg
    11341 gagctttaca tggtcttgga tttggtgata atgctaaggc agccatcatc gctcgaggtt
    11401 tagcagaaat cacccgccta ggggtagcac tcggggccag tccattgacc tatagcggct
    11461 tatctggtgt gggagatttg atcgtaacgg gaacttccat ccactctcgt aactggagag
    11521 ctggagatgc tctcggacga ggagaatccc tagctgatat agaagctaat atgggcatgg
    11581 taatcgaagg aatttcaacg actcgagcag cctatgaact agcccaagaa cttggagtct
    11641 atatgcccat tacacaggct atttaccaag ttatttatca cggaaccaat atcaaagatg
    11701 ccatttatga catcatgaac aatgaattta aagcagaaaa tgagtggtct taaccctcta
    11761 tagaaaggat ttttatgaca tcaaaagtta gaaaggcagt tatccctgct gctggactag
    11821 gaactcgatt tttaccagca accaaggccc ttgccaaaga aatgttgcca atcgtagaca
    11881 aaccaactat ccagtttatc gtggaagagg ctctcaaatc aggtattgaa gatattctag
    11941 ttgtcactgg taaatcaaaa cgttctattg aggaccactt cgattcaaac ttcgaattgg
    12001 aatataacct caaagaaaaa gggaaaacag atcttttgaa gctagttgat aaaacaactg
    12061 acatgcggct gcattttatc cgccaaactc atccacgcgg tctcggagat gctgttttgc
    12121 aagccaaggc tttcgtcgga aatgaacctt ttgtcgttat gcttggtgat gacttgatgg
    12181 atatcacaga cgaaaaggct gttccactta ccaaacaact catggatgac tacgagcgta
    12241 cccacgcgtc tactatcgct gtcatgccag tccctcatga cgaagtatct gcttacgggg
    12301 ttattgctcc gcaaggcgaa ggaaaagatg gtctttacag tgttgaaacc tttgttgaaa
    12361 aaccagctcc agaggacgct cctagcgacc ttgctattat cggacgctac ctcctcacgc
    12421 ctgaaatttt ccaaatcctc gaaaaccaag ctccaggtgc aggaaatgaa attcagctga
    12481 cagatgcaat cgacaccctc aataaaacac aacgtgtatt tgctcgtgag ttcaatgggg
    12541 ctcgttacga tgtcggagac aagtttggct tcatgaaaac atccatcgac tacgccctca
    12601 aacacccaca agtcaaagat gatttgaaga attacctcat ccaacttgga aaagaattga
    12661 ctgagaagga ataaaataaa ataaaaagat aaggttcaaa aaatgcctta tcttttttca
    12721 ttacacaaat tttctttcaa cttcgtcaca ttcccattcc cttgtagaaa agcaaaatgg
    12781 cgagtcctac gaacaagact accgctccta atctctggct ggtgttatac atccgttttt
    12841 ctcctctaac tggaaagata actgctagaa atgcgccacc aactgcacca ccgatatggc
    12901 ctgctaggct gattcctgga atcagaacac ttccaataat gttaaccaca aaaagtgtca
    12961 gataggattg ccctagctgt tggatataag gattgcgagt tgcatagcga agaacaataa
    13021 tcgcggcaaa tagcccataa agagaggtag aggcgcctgc tgctaaggat ttaggactaa
    13081 atacaaaaac aaagagattg cccatcattc ctgataaaag atagagaaag aaaaactgct
    13141 tagaaccgaa aatctcctct acctgccttc caagataata aagtgaaagc atattaacaa
    13201 tgaaatgttc ccacccaata tgaacaaaaa tggcagacaa gagacgccaa acctgctcgg
    13261 gaaagaggcg aatagctggc ccatacatgg ctccaaatcg aaataatgta tctgccctgt
    13321 caaagtttcc gcctgcagtg accaacatta gtaaaaatac caaggccgtc actaagagga
    13381 agaaactcgt cacagggtaa cgtctatcaa agatttcctt catcaattaa tacctcctga
    13441 acaggaatat catggttttc aggtataaag tcctgaattt gacaaggatg aaccgtactc
    13501 aaagtatgac cagaaaaatg ttccagatag cggtcataat aacctccacc atatccaatc
    13561 cgatatccat ccgtcgtaaa agccaggcca ggaacatgaa tcaaatcaat ctgagatgca
    13621 tccaccactt ccaaatctcc ctgtggctcc agtaaggcaa aggcagtttt taccaactgc
    13681 tgcggatcat agactacaaa gtccatgcgc cccttgggat aagttttggg tattaaaacc
    13741 ttcttgccgt ccttcagcgc ctgctcaatc agttcccgcg tttgaaactc atgagaaaaa
    13801 gagaggtagg ttgcgatgac cttggcttct tgataaaagg ggtgttgtaa aattcgctcg
    13861 gttaaagctt ggtctatagc ctgtttttgt tcttgagata aagccttcat ttcatgcaag
    13921 acttgcttgc gtaattccga tttcatagac aagccctcta ttctgctgcc ttctttttca
    13981 ggaaactaga caccgcagcc accccaatag ctaagacttc ttccttagga ctcatttgag
    14041 agtgatgaag agcgtaggga ctatcgatac ctagccaaaa catcacgcca tcaacctttg
    14101 aaaggagata accaaagtcc tcgcctgtca tagcaggttc gatatcaatc aactcgattc
    14161 cgtctttttc gtcaaagaag tccatcagtt cacgcgccaa ggctggattg ttctcaacag
    14221 gtaggtatcc accttgtttg agttccactt cgacttccat atcaaaggca gctgcaaccc
    14281 cttctgcaac tgtttttacc ctcttttgca ccaaaagact catgtcctgt gtcaaggcac
    14341 gaatagttcc atgtaaaaaa gctgtgtctg tgatgacatt gttggtggtt ccagcttgaa
    14401 aaatgccgaa ggtcaccact gctccctcga ttgggttgac attgcggcta acaactgact
    14461 gcacttgggt cacaaagtaa ctagccgcca ccaaggcgtc attggcttca tgaggaaaag
    14521 ctgcgtggcc atctttgcct ttgaaatgga tcttcacctc gcaagttcct gcaaagagtg
    14581 tatgagtatt agtcgcaatc tggccgactt tcaaatctgg acgaacatgg agaccataga
    14641 attggtttgg caaccaatct ccaaaagcac cgtcctcata catgagcata ccaccagctt
    14701 cattttcttc agcaggctga aatagaaaga gcagattatt cttgggttgc tcctcaaggg
    14761 cgcgctcaag acagcctaag gcaatggtca tatgaaaatc atggccacag gcatgcatgc
    14821 gaccttggtg ttgagaagca aaaggtagac ctgtttgttc gacgataggc aggccatcaa
    14881 tatctgtccg ccaaccaatg gttcgctccg gctgacttcc ctgcaggtag accaaaatcc
    14941 ctgtccgcca agtacgaatt tgaacaaaat ccttgcccgt agtcaatttc tcaatcacat
    15001 ccagcaaata agcctgagtc ttgaactcct ccaagccaat ctctggaatc tggtgtaaag
    15061 ctcgtctagt ctgaatcaaa tctaacatct atctgtcctc cgatatagca gaaagaggct
    15121 ggaaaaaggg ttccgcctct tttttacttt tacaattaca aggtacgaag cgcatcctct
    15181 agcgctgttt tttgttgggt ttgggcatca atttctttga tgatacgagc tggaacacct
    15241 gctactacca cgttttctga gacatcttgg gtaacgatag ctcctgcagc gacaactgaa
    15301 ccactaccga tttggactcc ttcgataacc actgcattag ctccgataag aacattgtct
    15361 ccgacacgga ctggttcagc actagctggc tcaatcacac ctgccaaaac tgcacctgca
    15421 ccaacgtggc tattttttcc aacgatggca cggccaccaa ggatggcacc catgtcaatc
    15481 atggttccag caccgatttc agcaccgata ttgataacag ctcccatcat gataacagca
    15541 ttgtcaccaa tttccacctg gtcacggata atagcacctg gctcgatacg agcgttgata
    15601 gcacgcttat ctagcaaagg aactgcagaa ttacgagcat cttgctcgac aacataatct
    15661 tgattttcta ccaaaccttc aagaagcgga gccacgtcct tccagtctcc gaataggaca
    15721 tttcctagtt tgacaacaga gctaggcaca gcagttgcga gttgcccctc aaaggttact
    15781 ttgacactgg ttttcttttc agcattggcg ataaattgga taatttcttg agcgttcatt
    15841 tttgtagcag tcataggtgc ctcctggttc gttataatga tacctattct accaaaaaag
    15901 gcctcaaatt tgaaaccttt ttgtctgttt ttaggtatga ttttttaggg atgtgagata
    15961 aaacgtgctg tcggtaaaag caagccccta ccgatacaac ctaaaaacac tcttcacaac
    16021 tatctccccg tgttttattg actattctag tatagcacac ttttagaatt aagaaaagac
    16081 tttttatcta ctttctttcg cttcttctat agacaaaaat atcatgggta aaataatcaa
    16141 ggctatagct agaaggaggg accaatccac tactaatcct aagaacaaaa cactcaagag
    16201 agcagaagag agaggttcac tggcactgat aacggaaacc accaaaggag aaaccaagga
    16261 cacagccttc atggaaatga aaaaagcaaa agccgttcca aagaaagcga taatgaggca
    16321 aatcaagata ctccaaatat caagagtaaa ggaaagctga taaaccggcg agaggacatt
    16381 gctaaacaaa cctgccaaaa tcatccccca cccaaccgta ggaacaaaac cataacgctt
    16441 agcaaaaggt tggggcaaga taacattaaa cataacaccc atggcactca gcaaacctgt
    16501 tataagagct agcggcgtca tggataactg agagaggtct ccctttgtcg ccatcaagca
    16561 aacacccagc atggcaacca aaacatagaa aacagcgctt tttgacgctc gtttttgata
    16621 aaccaagcga ttgtaaaaga ggataaagac agggctaata aactgtaaaa tagttgctgt
    16681 cgtagcattt gagtattcta cacagagata gaaaaaatac tgaactgaaa aaatccccaa
    16741 aatagcatag gctaaaaagg gcaggtaatt tttcttgtct cgccaaatat ctagcacttg
    16801 cgattttaat tgtattgcag accaaatgag tacaagactc cctgccagtg tcaaacgcat
    16861 agaggtaatc cagcccgaag acacctgata atgagtaaag aagtactctc ctaaaattcc
    16921 acagattccc catattaagc cggataggag cgaataaatt tttccgttaa caatcttttt
    16981 ctgatactga ttcataccct tattgtaaca cgaaaagaaa aaagtggcaa tccattgatt
    17041 ggccacttca tttagagaat tatcgtctcg cccttgaaga agaaggtcgt gtagtacttg
    17101 agttactgct atcgctagaa ctactacttg aactgctgga gctggatgga gttggtagac
    17161 tccccacaat actagaccaa gcattctgat aatccgcatc acttccacca atagcaaagc
    17221 gataacttgt cgctgtcgct cctgccttat tagcccaata gctggtaaca gtcgaacctg
    17281 tgacctctac ttcttttcct tcaacagaaa ccttccctgg tttttgacct gttgatttca
    17341 agacttccga tttcactaca ctaggatcta aagcaaagcg ctcgttcccc caaatgcttg
    17401 gggaagcttg ctgaatcgca tttaccagat gagccatgta attagagtta ttagaataac
    17461 ctgctctacg tgacaatgaa tgattatcat catgcccaat ccagccacct agggttaatc
    17521 taggtgtcga aagcatgagc cacatatttt cgtcttggtt ggttgtacca gtcttcccaa
    17581 tccaatctgc attagccaga gtaggattta aagaagtcag gttagacttg aaggttgttg
    17641 tcacacgaga ggatagaact tctcgtagca atccctgcat aatcgtcgca gtagcttttg
    17701 aatagacttg aaccggttta tcctgatact catacaccac tctaccatct gctgcttcaa
    17761 tctttgaaat cacatgcttc tgatgataaa ctccattatt agctaaggtc tgatagccat
    17821 tggtatgctg ggcaactgtg acttcaatac caccacccat tggcaagctc tcaataccgt
    17881 actcaggaat ctcgtaaccc atcttttcca tataaccctt gacatcaaca ccattttcac
    17941 ggagcatacg ataggtccag taagcaggga tattccatga atagttcaga gcttctccca
    18001 aggtcatcat tcctgttccc ttgctattag catacataat cggattgcca ttagcaaagt
    18061 ttgttggata gttagataga atcgtttcac ttcccatcaa gccctggtca atagcaatac
    18121 cgtaggccag caagggcttg gtagtagaag ctggcgaacg tttggtatca aaagcatgat
    18181 tattttgatt ttcttgataa ttacgaccac ctacaaagcc tagaatagca cctgtttggt
    18241 tgtccatcaa gacattccct acttctacac gacctgttcc atcgtctaaa agatagccat
    18301 aatcagcaac cgcactttgc atggcagaat gaattttctg atctatggta gtagtaatct
    18361 tataaccacc attttcaatt tccttggctg ccaaatctcg ataaaacttc tgagttgcct
    18421 catttttcaa ctccttagcg gagacattgt ctctctgagc tagatagtca tacatacgtt
    18481 cttgagcttc tgccaaagtt gtaaagtata aatagtctcg tgaaattcct gtaaccgtgc
    18541 ccgatggtaa aaagtcctgt ttaaggtcat aatccttgta ctgagaatac tcgtctttgc
    18601 ttaatgcacc tgtacgatac atactgtaaa gaactgcctt agcccgtctt aagccaattt
    18661 ctaggtcttc atcactcttc aactccccag tattttcata aggagagtaa gtaatgggac
    18721 tctgtggaag tcctgctaaa aatgctgctt gaggaacagt caactgactg gcatctacac
    18781 cgaaaattcc ctcagctgct tgccgagccc ctgcaatatt ctgtccctta ttatttcggc
    18841 caaagggagc cacattgaga taggtcgtta aaatctcatc tttattcatg gcgcgttcca
    18901 aggcaagagc atccacaatc tctgccgcct tacgagccaa ggtcggcgca tccccaacca
    18961 cctgctgttt aattagttgc tgggtcaagg ttgaaccccc actagaggaa gccaaaccta
    19021 caaatttccc caaggtcgca cgaatcaccg ccttgggtac tacaccctta tgttctttaa
    19081 agtgttcatc ttctgtcgca atgatagcct tcttcagatt ttccgaaatt tgctcagatg
    19141 agatagaagt gcgcaacaaa tcactctcta tggaagcaat caccgtcccg tccgaatagg
    19201 taatctctga aatagaagag atgtccttga cctgattcac caattcttct gtctgaggca
    19261 cccgaacctt gtcaaataag gccactccgt accccaaagc aatcccagct cccaacattc
    19321 ctcctagaaa accgagtaca aagagtaagt taaataaggc ttttatactc agtaaaatag
    19381 ctgggaaaat ggctgactta tctaaggttt tagatttttt ggtacttgaa cctttcttgc
    19441 caggtctagc tgatttttta tttttttgtt tttgctggaa aaattccagc atttttcgtt
    19501 ttaattcatt taattgattt tgcatggatt tcctcactct atctattata ccacaaaagg
    19561 gaaattttca ataaaatagc cactttcttc cctattctgc taggctattg cccaagtttg
    19621 tgatacaata ggtagaaaca ataattttaa aaaggagaaa agacacatgc acatttttga
    19681 tgagctaaaa gagcgtggtt tgatatttca aacgactgat gaagaagctt tgcgtaaagc
    19741 cctagaagaa ggtcaagttt cttactatac tggctacgat ccaactgctg acagccttca
    19801 cctaggccac cttgtcgcaa tcttgacaag tcgtcgcttg caactagcag gtcacaaacc
    19861 ttatgcgctc gttggcggtg ctacaggtct catcggagat ccgtccttca aagatgctga
    19921 acgtagtctc caaacaaaag acacagtaga tggctgggtc aagtctatcc aaggacaact
    19981 ttctcgtttt cttgactttg aaaatggcga aaacaaggct gtcatggtca acaactacga
    20041 ctggtttggc agcatcagct tcattgactt cctccgtgat attggaaaat acttcacggt
    20101 caactacatg atgagtaagg aatctgttaa aaaacggatc gaaacaggaa tttcttacac
    20161 tgagttcgct taccaaatca tgcaagggta tgacttcttc gtccttaacc aagaccataa
    20221 tgtcactctt caaatcggtg gttctgacca gtggggaaat atgacagctg gtaccgaatt
    20281 gcttcgtcgt aaggcggaca agactggtca cgttatcact gttccactaa tcacagatgc
    20341 aactggtaag aaatttggta aatcagaagg aaatgccgtc tggctcaatc ccgaaaagac
    20401 ttctccatac gaaatgtacc aattctggat gaacgtgatg gacgctgacg ctgttcgctt
    20461 cttgaaaatc tttactttct tgtcacttga tgagattgaa gatattcgca aacaatttga
    20521 agcagcgcca cacgaacgct tggctcaaaa agtcttggct cgtgaagttg ttacacttgt
    20581 tcacggagaa gaatcctaca aagaagcact taacatcact gagcaactct ttgcaggaaa
    20641 catcaaaaac ctttctgtca aagagctcaa acaaggactt cgtggtgtgc caaactacca
    20701 agtacaggca gacgaacaca acaatatcgt ggaactgctc gtctcatctg gtatagttaa
    20761 ctcaaaacgc caagcccgtg aagacgtcca aaacggagcc atctacgtaa acggcgaccg
    20821 catccaagac cttgactatg tcttgagtga cgctgataag ttagaaaatg aactgactgt
    20881 tatccgtcgt gggaagaaaa aatactttgt attgacttac taaactattc aacatttatc
    20941 tataaacaaa ggagttaacc tcgagaaagg taactccttt ttgctgttaa taactctcat
    21001 ctatctattt ttaatagaca ggctacgcag gacaatgcgc aaggttgtta gattatgtaa
    21061 gatagagaga tttgaaggac tgagccaatt aaataagcca aagccaatca aactactatt
    21121 tacgacaacg gtatcttgaa tatttttctt gatgagtgtt tgcaaagatg atgataacga
    21181 atccaactct tggaagaaat ccaaacgatt atctaacaaa taagatatca ctcatctgct
    21241 tagaaatatc tgcactctca ttcatcacca caccgatatc tgatagagtt aaagccgctg
    21301 agtcattcaa tccatctcca accatcaaaa tagtgtgacc tgctttctgc agtttctcta
    21361 ctaactcaaa tttcccatca ggtttcaagt ctgtatagac ctgatcaaag ggcaaatctt
    21421 tgactaattc ctctgtccta atcaaggtgt ctcctgttgc cagaatcaat tttttcccct
    21481 gtgccttaag tttatccaag gctgtttttg cttcttttct caaaggagta tgaatgcaga
    21541 acattccaat caattcattt tgataagcca agaataagag attgtagtga ctcttgtact
    21601 cttcaattaa agcattttgt tctgaactga tatgaatctg ctcatcctgc atcaagacat
    21661 aattcccaat aagaactggt tggccatcta tatgagattt gatccccttg cttgcgatat
    21721 attggagttt cccatgcatt tcctcatgtt caattccctc tatctcagct tgcttgacga
    21781 tggcattagc aataggatga taaatgtgtt cctcaagaca ggcactgatt ctgagaatat
    21841 cttcctcact atagtctcca aaaggtaaca ccttttcaac tataggataa ctagttgtga
    21901 ttgttcctgt cttatcaaac aagaaagtat caacttccag atatttctcc agaacatctc
    21961 catccttaat caccatttca cggttcaacc cttccttgat aactgtcaaa taagctacag
    22021 gagtagagat tttcaaagcg caggagaaat cgaccaatag gaaagaaata gccttagaaa
    22081 aagaacctgt caataggtaa gtcagcccag cccccaagaa attatattta acgactttat
    22141 ccgccatctt gatgaaatag cgttgtttcg ttttcttgtt ttcttcagat ttcttcatca
    22201 acttaatcag ctgtaaaata cggctgttca tctgattatc tgttacacga atgcgtaact
    22261 ctccagtttc taatactgta tttgcacaaa ccaaatcaga ctctcttttt tcaactggaa
    22321 aactctctcc tgtcaaggaa ctttcgttga ccatacctaa acctgaaact acttgtccat
    22381 caaacagaat ttcatttcct tgagataaga tcaagacatc tcctatttga acatcggaac
    22441 tcttgatact aacaaccgta tcgccctgta ctaggaatac atcgctctct tttgcaagaa
    22501 gactctgttc taaatctgtt gcagtttttt tcaaggacca ctgatctaaa tgattcccca
    22561 aatcaagcat aaacatgata ttgctagctg tcttggattg gttcataaac aaagacaata
    22621 aaatagccga acagtccaag acttccatcg ttagttcctt acgcgctagt gtttgatagg
    22681 cttctctaat ataacccaaa gcctgataac aagtccatat atagcgaata ggatacggca
    22741 caaaactacg aaaaagtaca cgcttaaccg ctgcacctga aacaatagaa taagcactct
    22801 cttctctacg aatgggaaga gtcatcaact cagaaacttt ccctttatca attcttttta
    22861 aaaaggcttc tgcattatct aatacagaaa agccttcttt tatgcgtaga gtaaagtgct
    22921 gttgatccat gtaaaactgg atagactcaa tccccttttc atctctcgcc aaggaacgaa
    22981 gatagtcttg aatatccaag gtaagtgaaa aagaagatga tagtcggata tgttggtatc
    23041 ctctatgtag cactttaaaa gacatattat tcacctataa ggctatctaa ttgctcttct
    23101 tttttctctt gctcgtacaa atatttggca tcttgcaaga catcgtctcc atgttgcttc
    23161 acaacagaaa cagatgcatc tagctcgtct ttcaacttgt aagccttagc caaagcttta
    23221 gaataacctt ttttagcttc cttacttgct aagattttca aaccaagggt accaaatgcg
    23281 acaccaccca aaaataatga agattttttc gcaacttttg caacggttaa tacttctttt
    23341 aacatactta tttcctcctt atcattatta tatagcaatt tagatataaa agcaatttca
    23401 ttctataaat tggagaatta gttttatttc tactgaatct cccatctact tattcctaaa
    23461 gttgctttca tttgcctctt ttgatacact taaactatga atacaaatct caagcccaaa
    23521 cttcagcgtt ttgcttctgc gactgccttt gcctgtccta tctgtcaaga aaatctgact
    23581 ctgttagaga ctaatttcaa gtgctgcaac cgtcattctt ttgacttggc gaaatttggc
    23641 tatgtcaatc tagcacctca aatcaagcaa tctgctaact atgacaagga aaattttcaa
    23701 aaccgtcaac aaatcctaga agccggcttt taccaagcta tcttagatgc tgtatctgac
    23761 ttgcttgcaa gctcaaaaac taccacaaca attttggata tcggttgtgg tgaaggattc
    23821 tattctcgca aactacaaga aagtcactct gaaaaaactt tctatgcctt tgacatctcc
    23881 aaagattcag tccaaatcgc tgctaaaagt gaacccaact gggcagtcaa ttggttcgtt
    23941 ggcgacttgg cacgacttcc tataaaagac gctaacatgg atattctgct tgatatcttc
    24001 tcacctgcca actatggaga atttcgtcgc gttttatcca aagacggtat cttgataaag
    24061 gttatcccaa ctgaaaatca cctcaaagaa atccgtcaaa gagtacagga ccagctgaca
    24121 aacaaggagt attctaacca agatatcaag gagcatttcc aggaacactt taccatccta
    24181 tctagtcaaa ctgcctctct gactaagact atcacagcag aacaactcca agccctactc
    24241 agtatgactc ctctcctctt tcatgttgac cagagcaaga ttgactggag ccaactgaca
    24301 gagattacca tcgaagcaga gattctggtt gggaaagcat tctaaactag acttcccaaa
    24361 ggtataaaac ttaccgaaaa tcttcaatat tacaaaaacg cataagaaca ggtacacaaa
    24421 ccttaatctt atgcgttttg ttatagtttc catacacatt aaggggaaat agttgaaatc
    24481 agttctctta ataaccattg aataccatat actacattta ccccaaatat gatataggcc
    24541 gtataaatca agtatttcga tttctcccgt ctcaaagaat agatattgta caaaatagat
    24601 aaatttaaaa taataaataa tagagacaaa atcttcctcc aaaatcaata ttaaaagtag
    24661 ccagtcaatc atagttctaa tctttatttt cgattcgaaa ttacaaactt atcaatgaac
    24721 aataagtgat ataaggccag taccaaaccc caaaagctac cacgaaaaga gaattcttga
    24781 tgattgagag taaacaaatc atggttacca atatagaggc aggtcaaaat aacaaataaa
    24841 atccaaaata aatccgtcca tttatatttt gaaaaatatt tttttaacat aatagcatat
    24901 ttttcttgac tataataaca gtatataata aaatgtaagc agatacaaat aaaaaggaga
    24961 aaagctcatg aacaaattaa tgaaatttat ttcggttttt ttgacgtcaa ttgtgttaat
    25021 tgtatcagcg attccaagtg tttcagctgt atacgcttct gaacaagtat cgcaaattga
    25081 aacaaatatg gaacttcaac ctgtcacttc tctaacagaa gaacaaatca atacacttgc
    25141 aaacgaaatc caatcttttc atccagacgt ctcacaacaa tggatcaaag aagtaattaa
    25201 ccgacaatta caaggcgatt atacaatccc acctacatac tctccattta gagcagcttg
    25261 gcaaggtatt acagttaatc aaatgggtgc tctattagat actacaatag ctttagcatt
    25321 aggaggaact actgcaggcc ttgcaaatct aattaaagta aaaggaaaac atgcagcaaa
    25381 aagtgctatt cgttcagcaa tttctagata tctaggtagt tggtttgtaa atgatgttgc
    25441 tttagaatcc gctatgaatt tattatcacc ggggacttat ttagcacaat tatgggataa
    25501 aaatgatgcc attcctaaca acggaaggat taacttttaa tcatgagata tacaataaaa
    25561 ttaattgggt ataacgtatt ttcgttgttt atattcttta tcttatcaat gttgtttaaa
    25621 gttcccttta ggtggtggat cagcattgca atgacgactt cgtcgtacat tgtatataat
    25681 tacttaaaga aaaagagaga aaaggaatga tcttatacca gatcattcct tttctgcttt
    25741 ctgtaaatcc ctcggagtgt gtttggcgct ttgatacgaa gtgtcaaagc tgtataaacg
    25801 ttactatttc caaaaaagta acagaaaatt cctgctttgt aggactgctt tgggtgagta
    25861 atagacatgt cgactcaccc tatctcctga tggcgaaaag aatttagcaa tggtgcaagt
    25921 tgttctgaac tcgagaaaaa acatgccgaa atcgaagaaa agcgtgcaga acttgaagcg
    25981 aagaagcaag aaatgttgaa gcgcttggat aaatagaatt tagaaaacag aaaaaactaa
    26041 aaagaatgta gggtttattt ccctacattc ttaaaataac ttagtacata aatgacaatg
    26101 ctgccactgc cgtcaatatt gcaaaaacta ttttccaaat ttttttattt tttccaaaaa
    26161 tcttatcagg taacaagctt attacaagaa aggtaataaa caacaccaat ttaagtgtgt
    26221 atatcataaa aacaccttct ttcctacaat tatattataa tactttatgt aagcgaatac
    26281 aagaggtgtg aggtaatcaa ctacgaaaaa atataaggct gaggacagta aaaaccagca
    26341 tttaaagtct tcactgtatc taggatttcc ctatttttag acaagattga cccaaatcta
    26401 cagcatttaa aaaagcgaat ttcaatcgaa actcgctttt ttacaaaatg tattagtata
    26461 aatttgtatc ttatactcaa tgaaaaccaa agagtaaact aggaagctag ccgcaggttg
    26521 ctcaaagcac cgctttgagg ttgcagataa agctgacgtg gtttgaagag attttcgaaa
    26581 agtattagtt caagtgccag atgtcttcgt tatactgagc gattgtacgg tcagatgaga
    26641 agaatcctgc tttagaaatg ttaacgatga ctttatccaa ccatgcgtca cggtcttcgt
    26701 agtcagcaag catttgctct ttgactttga tgtagtcttc caagtcaaga agagtcatga
    26761 accagtcttt gttgatcaat tcattgtaaa gacgttccaa gcgctctttg tttccagctg
    26821 caagaactgc atcactaacg atgaagtcaa ccaatggttt gatagcttca cgagcgtaga
    26881 attcgcttga tttgtaagct gcttttgcgt aaaggtcgat aacagtttct gaatcttcac
    26941 cgaagatgta gatgttttct tctccaacca actcagcgat ttccacgtta gcaccgtcca
    27001 tagtaccaag tgtcaaagct ccgttcaaca tgaatttcat gttaccagta cctgaagctt
    27061 ctttagaagc aagtgagatt tgttctgaga tatcacatgc tgggataagg aaacttgctg
    27121 cagtaacgtt gtagttttca accataacta cttgcaagtg tggagctact gctggatcgt
    27181 tagcaataac ttctgacatg caaaggatta aatggataat gtcttgagcg attgtgtagg
    27241 ctggagctgc tttaccacca aagaagattg tgattggacg agcagggatg ttaccagctt
    27301 tgatgtcaag gtatttgtgg atcacgtaca aagcgttcat ttgttggcgt ttgtactcgt
    27361 gaagacgttt gatttggata tcaaagatag aatttggatt gatttccaca ccttggtgtt
    27421 ctttcaagtg acgagccaat ttacgtttgt tgtgagcctt gatgctttcc aatttttctt
    27481 tgacagctgc tttgtcttca taagacaaaa gtttttcaag ctcatctgct tcatggtgcc
    27541 aaccatctcc aagaatctca tccaagtagt gagacaatct tgggttagca tgcataagcc
    27601 aacgacggaa agtgataccg tttgttttgt tgttgaactt ttctgggtaa aggtcgtaga
    27661 aggctttcaa ctcagaattt ttcaagattt cagtatgaag tgctgcaacc ccgttaacac
    27721 tgtatccgta gtggatatcc atgtgagcca tgtgaacacg tccgctctca tcgatgattt
    27781 gaacagctgg atctttgtac tctgccttca cacgacggtc caattcttcg atgattggta
    27841 ccaagtgagg aaccacttct tgcaagaatt caagaggcca tttttcaagc gcctcagcaa
    27901 ggattgtgtg gttagtgtag gcagtcatgc tacgaacaat tgagattgct tcgtcaagct
    27961 cgataccacg tgcagtcaaa agacgaatca attcaggaat caccattgat gggtgagtat
    28021 cgttgatttg gacaactgcg tagtcagcaa ggtcatgcaa gttgcttcct ttttcgattg
    28081 cttcgtcgat gatcaattgc gcaccgtttg aaaccatgaa gtattgttgg aagatacgga
    28141 gcaattcacc ttgacggtca ctatcatctg ggtaaaggaa gagagttaag ttgcgagcga
    28201 tatctgtctt gtcaaagttg ataccatctt taataataga agaatcaact gaatccaagt
    28261 caaacaaacg caagcggttt ttagtcgctg tttcataacc agtaacatca atatcgtaaa
    28321 gagttgatgt caaagtaaag tctgcaaatg gtacttggta gctacggctt gagcgaacca
    28381 accagttttg ctctgtcaac catgcatttg gaattgtttc ttgttggttg tttttaagaa
    28441 cttgttggaa aagaccaaag tggtagttaa gaccaacacc gtcaccattc aaaccaagag
    28501 tagcaattga gtcgataaag caggcagcca aacgtcccaa accaccatta ccaagagatg
    28561 gttccaattc aacttcttca acttcgatca agtctttacc tgcagctgca agttcttttt
    28621 taacatcgtc gtaaagacca aggttaatca agttgtttga caagagttta ccaatcaaga
    28681 actcagctga gatgtagtaa acttttttct taccagtgtt gactggtttt tggctgcttg
    28741 caagcttgct gtagttaaga agagcaaggt aaagctcttc attgctacat tctgcaatgg
    28801 ttttattgta acgattttgt acaaattctt gtagtgataa catgtttagt tgtctcctga
    28861 ttattgtctt atttctttaa atctaccaaa ttttcattaa ttcggcgata aattgttgtc
    28921 aagtcaagca aaccttcctc gacagctggt gtcaattgat cttcagtcat acgccaagac
    28981 cagtttccac caagggtaga tgggaagttc atacgagctg cctcatccaa ttctagtaaa
    29041 tcttgcatag ttgcaattgc cataaagcta actgatgaaa atactgtacg aagcatagcg
    29101 tgtaccactg tttcgtattc tttacggttc gtgtaacgag ccatgtactc acgagtcgca
    29161 tcatcaatct cattacggta ccaaccaaga accgtattgt tatcgtgtgt tcctgtgtac
    29221 ataactgagt tagcaggtgc caagtgtggg ctatcaatgc tttcgtcttc tgggttgaag
    29281 gcaaattgaa gaatcttcat tcctgggaag ccagtacgtt cacgcaattc gatcacttca
    29341 tctgtcatga agccaaggtc ttctgcgatg atgtttagct caccaagttc ttccttaacg
    29401 gctgcaaaaa gcttgtagcc tggacctttc acccactcac caggtgctgc tgtatcggaa
    29461 ccagcaggga tttcccagta agattcgaag ccacggaagt ggtcgatacg aacgatatcg
    29521 tagattttga agctttcacg caagcgttca atccaccatt tgtagccgtc tttgtccatt
    29581 gcttcccagt catagattgg attaccccaa agctgaccag ttacagaaaa ctcatctggt
    29641 gggcatcctg cgatacaagt agccttacca ttgacatctg ttttgaagag atgtggattt
    29701 gcccacatat cacttgaatc ttccgctacg tagattggca tgtccccaac gatttcgatg
    29761 tggttgtcgt tagcgtaagc tttcaatttc aaccattgtt ggaagaagaa gtattgagtc
    29821 acacggtggt aaaccaactt gtctgccaat tgctcacgat agctttcaag tgctgaagct
    29881 ttacgagcac gagcatctgc atctggccat tcagtccaag caagattgtc aaaatactct
    29941 ttgatagcca tatactcagc aaagagctca agccatgatt ggttgtcttg agcaaatttc
    30001 tcaaaatctt taacatctcc gacttcaaag aaacgtttca ccgctttttc taaaagagga
    30061 cgacgtgcat agtagatttt agcatagtca acttcagacg catcgctacc aaagtcaact
    30121 ccttcaaggt cacttgcttc caacaaacct tgctccacca agatatctaa atcgataaaa
    30181 tgagtgtttc ctgcgaaggc tgagaaagat tggtaaggag aatccccgta actagttgct
    30241 cctaatggaa ggatttgcca gtaacgttgt tttgtacgga ccaagaaatc aacgaagtcg
    30301 taagcacttt gaccaaatga tccgattccg taagctcctg gaagagaaga gatgtgcatc
    30361 aacacaccac tttgacgttt tttcataata gcacctcgtg tgttaaaata atggaacgtt
    30421 gcgtattttg cagacgcaaa cgtttgcgtt acttataagt atactccttt tcaagcataa
    30481 ttgcaagcgt ttttaaaaaa cttttttgat tatttttaag gagactaact cattttagca
    30541 aagataactt aaaactatct aatcctaaag tttgactctc acgagacagt ctacaaagta
    30601 caaaacctcc ttagggagct tgaatttata tgcaacaaag cacaaacgtc tatagaaaat
    30661 agatagggat tagaaacagg gaggtagccc ctcctgattt ccctctttag acagattcca
    30721 tatattttta ttaaaaatac tgtattctta tctatttcat acttttcttc tattaaaatg
    30781 caaaattatg ctatcaaatc aagcttaaaa attttttaaa atttttacaa aaaatacttg
    30841 caaccgtttt ctatttgtgc tatactaagc tcataaagga aaacgtttgc gtttccttat
    30901 caataaaact tgctattctt tgggaggaat acactatgtc atctaaattt atgaagagca
    30961 ctgcggtgct tggaactgct acacttgcta gcttgctttt ggtagcttgc ggaagcaaaa
    31021 ctgctgataa gcctgctgat tctggttcat ctgaagtcaa agaactcact gtatatgtag
    31081 acgagggata taagagctat attgaagagg ttgctaaagc ttatgaaaaa gaagctggag
    31141 taaaagtcac tcttaaaact ggtgatgctc taggaggtct tgataaactt tctcttgaca
    31201 accaatctgg taatgtccct gatgttatga tggctccata cgaccgtgta ggtagccttg
    31261 gttctgacgg acaactttca gaagtgaaat tgagcgatgg tgctaaaaca gacgacacaa
    31321 ctaaatctct tgtaacagct gctaatggta aagtttacgg tgctcctgcc gttatcgagt
    31381 cacttgttat gtactacaac aaagacttgg tgaaagatgc tccaaaaaca tttgctgact
    31441 tggaaaacct tgctaaagat agcaaatacg cattcgctgg tgaagatggt aaaactactg
    31501 ccttcctagc tgactggaca aacttctact atacatatgg acttcttgcc ggtaacggtg
    31561 cttacgtctt tggccaaaac ggtaaagacg ctaaagacat cggtcttgca aacgacggtt
    31621 ctatcgcagg tatcaactac gctaaatctt ggtacgaaaa atggcctaaa ggtatgcaag
    31681 atacagaagg tgctggaaac ttaatccaaa ctcaattcca agaaggtaaa acagctgcta
    31741 tcatcgacgg accttggaaa gctcaagcct ttaaagatgc taaagtaaac tacggagttg
    31801 caactatccc aactcttcca aatggaaaag aatatgctgc attcggtggt ggtaaagctt
    31861 gggtcattcc tcaagccgtt aagaaccttg aagcttctca aaaatttgta gacttccttg
    31921 ttgcaactga acaacaaaaa gtattatatg ataagactaa cgaaatccca gctaatactg
    31981 aggctcgttc atacgctgaa ggtaaaaacg atgagttgac aacagctgtt atcaaacagt
    32041 tcaagaacac tcaaccactg ccaaacatct ctcaaatgtc tgcagtttgg gatccagcga
    32101 aaaatatgct ctttgatgct gtaagtggtc aaaaagatgc taaaacagct gctaacgatg
    32161 ctgtaacatt gatcaaagaa acaatcaaac aaaaatttgg tgaataaaaa atttgttcaa
    32221 ggggggtgga aatcaaatcc ccctttgaat ttatcaatag agacacaaat aatttagctt
    32281 tcttataaaa aagtagtatc ctatgaaagg agttaatatg gaaaagcaac aacctagtaa
    32341 agcagccctg ctgtctatca ttcctgggtt aggacagatt tacaataaac aaaaagccaa
    32401 aggttttatc ttccttggtg taaccatcgt atttgtcctt tacttcctag cacttgcaac
    32461 ccctgaattg agcaacctca tcactcttgg tgacaaacca ggtcgtgata attccctctt
    32521 tatgctgatt cgtggtgcct tccatctaat ctttgtaatc gtttatgtac tcttttattt
    32581 ctcaaatatc aaagatgcac atacgattgc aaaacgcatt aacaatggaa ttccagttcc
    32641 acgcacactc aaagacatga tcaaagggat ttatgaaaat ggcttccctt acctcttgat
    32701 cattccatct tatgttgcca tgaccttcgc gattatcttc ccagttatcg taaccttgat
    32761 gatcgccttt accaactacg acttccaaca cttgccacca aacaagttgt tggactgggt
    32821 tggtttgacc aactttacaa acatttggag cttgagtacc ttccgttctg cctttggttc
    32881 tgttctttct tggactatca tttgggcttt ggcagcttct actttacaaa tcgtaattgg
    32941 tatcttcaca gctatcattg ccaaccaacc atttatcaaa ggaaaacgta tctttggtgt
    33001 tattttcctt cttccttggg ctgtcccagc cttcatcact atcttgacat tctcaaacat
    33061 gtttaacgat agtgtcggtg ctatcaacac tcaagtattg ccaatcttgg ctaaattcct
    33121 tcctttcctt gatggagctc ttattccttg gaaaacagac ccaacttgga ctaagattgc
    33181 cttgattatg atgcaaggtt ggctcggatt cccatacatc tacgttttga ctttgggtat
    33241 cttgcaatct attcctaacg acctttacga agcagcttat attgacggtg ccaacgcttg
    33301 gcaaaaattc cgcaacatca ctttcccaat gattttggct gttgcggcac ctactttgat
    33361 tagccaatac accttcaact ttaacaactt ctctatcatg tacctcttca atggtggagg
    33421 acctggtagt gtcggaggtg gagctggttc aaccgatatc ttgatctcat ggatctaccg
    33481 tttgacaaca ggtacatctc ctcaatactc aatggcggca gctgttacct tgattatctc
    33541 tatcattgtc atctcaatct ctatgatcgc attcaagaaa ctacacgcat ttgatatgga
    33601 ggacgtctaa gatgaataac tcaattaaac tcaaacgtag actgactcaa agccttactt
    33661 acctttacct gattggtcta tcaattgtaa ttatctatcc actgttgatt accatcatgt
    33721 cagcctttaa agcaggtaac gtctcagcct ttaaactaga tactaatatc gacctcaatt
    33781 ttgataactt taaaggcctc ttcactgaaa ccttgtacgg tacttggtac ctcaacactt
    33841 tgattatcgc cttaattacc atggctgttc aaacaagtat catcgtactt gctggttatg
    33901 cttacagccg ttacaacttc ttggctcgta aacaaagttt ggtcttcttc ttgatcatcc
    33961 aaatggtgcc aactatggcc gctttgacag ccttcttcgt tatggcgctt atgttgaacg
    34021 cccttaacca caactggttc ctcatcttcc tctacgttgg tggtggtatc ccgatgaatg
    34081 cttggctcat gaaaggctac ttcgatacag tgccaatgtc tttagacgaa tctgcaaaac
    34141 tagacggtgc aggacacttc cgccgcttct ggcaaattgt tctaccactt gttcgcccaa
    34201 tggttgccgt acaagctctc tgggccttca tgggaccttt cggggactac atcctctcta
    34261 gtttcttgct tcgtgagaaa gaatacttta ctgttgccgt aggtctccaa accttcgtta
    34321 acaatgcgaa aaacttgaag attgcctact tctcagcagg tgctatcctc atcgcccttc
    34381 caatctgtat tctcttcttc ttcctacaaa agaactttgt ttcaggactt acaagtggtg
    34441 gcgacaaggg ataatttatc cccgccaccc tttttcattt tatactcttc gaaaatctct
    34501 tcaagccacg tcagctttat ctccaacctc aaagttgtgc tttgagcaac ctgcagctag
    34561 cttcctggtt tgcactttga ttttcattga ctattagcga ttgtcactgt aaataatatc
    34621 cttgtagcaa gcaatttacc ccctagactt gaaataaagc gcatttctct atataataat
    34681 actcatatag aaaacacctt ttagaaagat acctatgctt ccatatccat tttcctattt
    34741 ttcaagtatt tgggggtttc gtaagcccct gtccaaacgt ttcgagctca actggtttca
    34801 acttctcttt accagtatct tccttatcag cttgtctatg gtacccattg ctatccaaaa
    34861 cagctcccag gagacctatc cgctagaaac ttttatcgat aatgtctatg aacctctgac
    34921 aaatgaggtt gtccaggatc tctctgaaca tgctacaatt gtcgatggca cattaactta
    34981 tactggaaca gctagtcaag ccccttctgt tgtgattggt ccaggtcaaa tcaaggaatt
    35041 acctaaggac ttgcaactgc atttcgatac aaatgaacta gtcatcagca aggaaagcaa
    35101 ggaactgacc cgcatctctt accgagccat tcagactgag agtttcaaaa gcaaagacag
    35161 cttgacccaa gcaatttcta aagactggta ccaacaaaat cgtgtctata tcagcctctt
    35221 cctagttctc ggtgcgagct tcctctttgg tttgaatttc tttatcgtct ctctaggagc
    35281 tagtcttctc ctttatatca ctaagaaatc acgcctcttt tcatttagaa cctttaaaga
    35341 gtgctatcac tttatcttga actgtttagg attgccgact ctgattacac ttattttggg
    35401 attatttggc caaaatatga caaccctgat tactgtacaa aatattcttt ttgttctgta
    35461 tctggtcact atcttttata aaacacattt ccgtgatcca aattaccata aataggagat
    35521 ttttatgccc gttacgatta aagacgtggc caaggctgct ggtgtttcgc cttcaaccgt
    35581 aacccgtgtt attcaaaata aatcaaccat tagcgacgaa acaaaaaaac gcgttcgcaa
    35641 agctatgaag gaactcaact accacccaaa ccttaacgct cgtagcttgg taagcagcta
    35701 tactcaggtt atcggattag ttcttcctga tgactcagac gccttctacc agaatccttt
    35761 ctttccatcg gttctacgtg gcatctctca agtcgcatct gaaaaccact atgccattca
    35821 aatagcaaca gggaaagatg agaaggagcg tctcaacgct atttcacaaa tggtctacgg
    35881 caagcgtgta gatgggctaa tctttctcta tgcccaagaa gaagaccctc tcgtaaaact
    35941 cgtcgcagaa gaacagttcc ccttccttat cttaggtaaa tctctatctc ctttcatccc
    36001 acttgtcgac aacgacaatg ttcaagctgg ttttgatgcg actgaatatt tcatcaaaaa
    36061 aggctgcaaa cgcattgcct ttatcggagg aagtaaaaag ctcttcgtga ccaaagaccg
    36121 tttaacgggc tatgaacagg cgcttaaaca ttacaaactt accactgaca acaatcgcat
    36181 ctactttgcc gacgagtttc tggaagaaaa gggctataaa tttagcaagc gattattcaa
    36241 gcacgatcca caaattgatg ctatcatcac aaccgatagc ctcctagctg aaggtgtttg
    36301 taactatatt gccaaacacc agctggatgt ccctgttctc agctttgact cggttaatcc
    36361 caagctcaac ttggcagcct atgtcgatat caatagttta gagcttggtc gtgtttccct
    36421 tgaaactatt ctccagatta ttaatgataa taaaaacaat aaacaaattt gttaccgtca
    36481 attgatcgcc cacaaaatta tcgaaaaata agagactggg caaagagtcg ttaaaagcaa
    36541 aaacgcatac tatcaggtat tgaaaaaact tgatactatg cgttttattg tgggaagatt
    36601 tacttccttt tctactgaaa ttgagtcttt tcccaagatc tttttatact caatgaaaat
    36661 caaag
//
LOCUS       DAVMMR010000017        35362 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMMR010000017 DAVMMR010000000
VERSION     DAVMMR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.017"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(100..450)
                     /gene="rbfA"
                     /locus_tag="VYS74_000804"
     CDS             complement(100..450)
                     /gene="rbfA"
                     /locus_tag="VYS74_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV3788867.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(701..3493)
                     /gene="infB"
                     /locus_tag="VYS74_000805"
     CDS             complement(701..3493)
                     /gene="infB"
                     /locus_tag="VYS74_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV3788868.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3510..3809)
                     /locus_tag="VYS74_000806"
     CDS             complement(3510..3809)
                     /locus_tag="VYS74_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV3788869.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3802..4095)
                     /locus_tag="VYS74_000807"
     CDS             complement(3802..4095)
                     /locus_tag="VYS74_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV3788870.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4117..5253)
                     /gene="nusA"
                     /locus_tag="VYS74_000808"
     CDS             complement(4117..5253)
                     /gene="nusA"
                     /locus_tag="VYS74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV3788871.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5297..5776)
                     /gene="rimP"
                     /locus_tag="VYS74_000809"
     CDS             complement(5297..5776)
                     /gene="rimP"
                     /locus_tag="VYS74_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV3788872.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDGLTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5902..6537)
                     /gene="trmB"
                     /locus_tag="VYS74_000810"
     CDS             complement(5902..6537)
                     /gene="trmB"
                     /locus_tag="VYS74_000810"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV3788873.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6534..7328)
                     /locus_tag="VYS74_000811"
     CDS             complement(6534..7328)
                     /locus_tag="VYS74_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV3788874.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7489..8100)
                     /locus_tag="VYS74_000812"
     CDS             complement(7489..8100)
                     /locus_tag="VYS74_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3788875.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            complement(8251..8484)
                     /locus_tag="VYS74_000813"
     CDS             complement(8251..8484)
                     /locus_tag="VYS74_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788876.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(8526..9215)
                     /locus_tag="VYS74_000814"
     CDS             complement(8526..9215)
                     /locus_tag="VYS74_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3788877.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            complement(9267..9650)
                     /locus_tag="VYS74_000815"
     CDS             complement(9267..9650)
                     /locus_tag="VYS74_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV3788878.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10279..10650)
                     /locus_tag="VYS74_000816"
     CDS             complement(10279..10650)
                     /locus_tag="VYS74_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788879.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(11118..11237)
                     /locus_tag="VYS74_000817"
     CDS             complement(11118..11237)
                     /locus_tag="VYS74_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV3788880.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11341..11490)
                     /gene="blpO"
                     /locus_tag="VYS74_000818"
     CDS             complement(11341..11490)
                     /gene="blpO"
                     /locus_tag="VYS74_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV3788881.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11734..11982)
                     /gene="blpK"
                     /locus_tag="VYS74_000819"
     CDS             complement(11734..11982)
                     /gene="blpK"
                     /locus_tag="VYS74_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV3788882.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(12051..12320)
                     /gene="blpJ"
                     /locus_tag="VYS74_000820"
     CDS             complement(12051..12320)
                     /gene="blpJ"
                     /locus_tag="VYS74_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV3788883.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(12787..12984)
                     /gene="blpI"
                     /locus_tag="VYS74_000821"
     CDS             complement(12787..12984)
                     /gene="blpI"
                     /locus_tag="VYS74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV3788884.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            13266..13853
                     /locus_tag="VYS74_000822"
     CDS             13266..13853
                     /locus_tag="VYS74_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV3788885.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            13780..15423
                     /locus_tag="VYS74_000823"
     CDS             13780..15423
                     /locus_tag="VYS74_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV3788886.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENHYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            15434..16795
                     /locus_tag="VYS74_000824"
     CDS             15434..16795
                     /locus_tag="VYS74_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV3788887.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            16852..16980
                     /gene="blpC"
                     /locus_tag="VYS74_000825"
     CDS             16852..16980
                     /gene="blpC"
                     /locus_tag="VYS74_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV3788888.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(17051..18391)
                     /locus_tag="VYS74_000826"
     CDS             complement(17051..18391)
                     /locus_tag="VYS74_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3788889.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(18405..19142)
                     /locus_tag="VYS74_000827"
                     /pseudo
     CDS             complement(18405..19142)
                     /locus_tag="VYS74_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(19147..19485)
                     /locus_tag="VYS74_000828"
     CDS             complement(19147..19485)
                     /locus_tag="VYS74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3788890.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            19761..20090
                     /locus_tag="VYS74_000829"
     CDS             19761..20090
                     /locus_tag="VYS74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV3788891.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(20258..21307)
                     /locus_tag="VYS74_000830"
     CDS             complement(20258..21307)
                     /locus_tag="VYS74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788892.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(21304..22035)
                     /locus_tag="VYS74_000831"
     CDS             complement(21304..22035)
                     /locus_tag="VYS74_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788893.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYISETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            22103..22513
                     /locus_tag="VYS74_000832"
     CDS             22103..22513
                     /locus_tag="VYS74_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3788894.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            22523..22810
                     /locus_tag="VYS74_000833"
     CDS             22523..22810
                     /locus_tag="VYS74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788895.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(23500..24636)
                     /gene="dnaJ"
                     /locus_tag="VYS74_000834"
     CDS             complement(23500..24636)
                     /gene="dnaJ"
                     /locus_tag="VYS74_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV3788896.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(25038..25394)
                     /locus_tag="VYS74_000835"
     CDS             complement(25038..25394)
                     /locus_tag="VYS74_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV3788897.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(25396..27219)
                     /gene="dnaK"
                     /locus_tag="VYS74_000836"
     CDS             complement(25396..27219)
                     /gene="dnaK"
                     /locus_tag="VYS74_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV3788898.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(27699..28223)
                     /gene="grpE"
                     /locus_tag="VYS74_000837"
     CDS             complement(27699..28223)
                     /gene="grpE"
                     /locus_tag="VYS74_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV3788899.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(28250..29284)
                     /gene="hrcA"
                     /locus_tag="VYS74_000838"
     CDS             complement(28250..29284)
                     /gene="hrcA"
                     /locus_tag="VYS74_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV3788900.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(29452..29958)
                     /locus_tag="VYS74_000839"
     CDS             complement(29452..29958)
                     /locus_tag="VYS74_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV3788901.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFCFICFSDFLSNSWLFQ
                     TKKEIPSR"
     gene            30566..32899
                     /locus_tag="VYS74_000840"
     CDS             30566..32899
                     /locus_tag="VYS74_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3788902.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            32912..34375
                     /locus_tag="VYS74_000841"
     CDS             32912..34375
                     /locus_tag="VYS74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3788903.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            34375..>35362
                     /locus_tag="VYS74_000842"
     CDS             34375..>35362
                     /locus_tag="VYS74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3788904.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT        11060 a         7394 c         6322 g        10586 t
ORIGIN      
        1 ggcgtactat tctagtttca atatatttta aaagaatatt tttccatttc aaattcaacc
       61 tattttcctc caccaaaaaa gaggggcaac ccctcttctt tagttcttat ccagattgcg
      121 tagcatctcg tcaatcttgt ttccatactc gatggactcg tctttgacga aggtcaaatc
      181 tgggattttg tacaatttca aattgcgacc aagttcacgt ttgatggtac cagttgcttt
      241 ttcaagcccg atttgggctt tttggttatc cgaagcaagg ttactcaaaa tggtgtaata
      301 aaccttggca acagacaagt cacccagcat ctgaacatct gtgatggtca caccttggac
      361 acgtggatca cggactttct tttgcaaaat ctcattgact tcacgcttga tttccatgcc
      421 cacacgatct gtacggaaat gatttgccat gatattcctt tctctacttt gaaccaaaag
      481 ctaggccagc agacctagct attttaaact tcttgatttt cagtttaaat tgaaacgaag
      541 ttcaatttga agtcatctgc ttctttcgcc gtggtgaaag tgatggaact gatttatcag
      601 tcccatcaca ggggattttt gagaccctag gctcaaaaat aagcaatgaa accatcggtt
      661 tgcttgcgtc cctcaccacc taagaaagga gcaaaaaatc ttatctcttg atttcttcca
      721 tgacatacgc ctcaatcaca tcatccatct taatatcatt gtagccgtcg atcatcaatc
      781 caccttcacg accgtttgtc acttctttca cgtcgtcttt gtagtgtttc aagcttgcga
      841 gttcgccatc atagataacg acaccatcac ggataacacg gactttagag tcacgggcaa
      901 ccttaccgtt gataaccata aatccaccga tagttcccac tttagacacc ttgaaggttt
      961 cacggataac cgcttcacca ataacttttt cttcaaattc tggatcaagc atccctttca
     1021 tagcttcttc catctcttcg ataaccttgt agataatgct gtgaagacgg atttccacat
     1081 cgtcagcttc tgcttgttga cgagcttgtg gtgtagggcg tacgttgaaa ccaacgataa
     1141 aggcatttga agcttcggca agggtcacgt ctgattcgtt gatagcaccg accgctgagt
     1201 ggacgatagt cactttgaca ccttccacgt caatcttttg aagtgaggca gaaagggctt
     1261 caacagaacc ttgtacatca gccttgatga taacattaac agatttgagt tccccagctt
     1321 taagggtatc aaagaggttt tcaaggctaa cacgttgggt agcttgacgt tgtttcatga
     1381 gggcacgttt ggcacgctct tcacctgctg cacgcgcaga tttttcatcc tcgtaaacgg
     1441 caaagtggtc acccgccatc ggtgcttcgt tcaaacctgt gatagagact ggtgttgatg
     1501 gtccagcaac tttaacacga cgaccaaggt cgttggtcat agcacggaca cgaccgaagg
     1561 tatttccgac aacgattgga tcttgaacat tcaaggtacc ttgttgtaca agaagggttg
     1621 cgaccgcacc ttttccttta tccaagcgcg cttcgataac cgtaccgatt gcacgcactg
     1681 ttgggtctgc cttgagttct tggatttcag ctacaagaag gactgtttcc aacaattctt
     1741 cgatattttg gttgaattta gctgagattt caacaaattc agaatctcca ccccaagcgg
     1801 ttgacatcac accatgctct gccaattcac cgataacgcg ttctgggtta gcacctggtt
     1861 tatcaatctt gttaatggct acgatgattg gaacgttagc tgcttttgag tggttgatgg
     1921 cttcaatagt ctgaggcata accccgtcat ctgccgctac gaccaagatc gtaatatcgg
     1981 taacagaagc accacgcgca cgcattgatg taaaggccgc gtgtcctggt gtatcaagga
     2041 aggtaatctt cttaccattt tccacgattt ggtaggcacc gatatgctga gtaataccac
     2101 ctgcttcacc tgtcgcaaca cgtgagttac gaagagtatc caaaagggtt gttttaccgt
     2161 ggtcaacgtg tcccatgata gtaacaactg gtggacgctc aaccaattca tcttcattga
     2221 gataaccatc ttcgacaaag aaacgttcga tgtcagcatt atccacttca accttttgtt
     2281 tggcttcgat accgtaatcc accatgagga gttcaattgt ttccccatcc aaggattggt
     2341 tttgtgtggc catgacaccc atcatgaaaa gtttcttaac aatttcagct ggttcacgtt
     2401 tgatacgttt tgcgatttcc gcaacggtca taccatctgt atattcaaat tctgttggca
     2461 attcatggaa tttacgctcc gtaacaggtt ttggagtctg attacggttg ttcttgttat
     2521 tgcctttttt gttctttttg ttgttattcc agttactatt cttttgattt ctcacttgat
     2581 tttgactact tcgattcttt tgttgttttc taggaccatc ttcttcatga tcataatcgt
     2641 cacgattttt gtctggtcga gcttgttttt tacgacgtgt atccactgca ggttcttttt
     2701 tctcagggac gacttcaacc actgcttgaa cttgctgtgc ttgttcagct aacttagccg
     2761 cttcttcaaa gatttcctct ggttccttgc gtttgttagc ttgagccaag gcttctttag
     2821 cagcctgata ctgcttgaag cgttcctcac ttgaacgagc gtactctgca ttttgctctg
     2881 cttttagggc tgctgcacgg gctttaaagt caatacgtgg agccgcttga tttgaacgtt
     2941 tatcctcttg ctgacggcgc tcatttctac gtttttgctg accttgctgc ttcttagctt
     3001 ggtcattaaa gcgacgattg tcgcggttgc tttgaccttg ttttccacca ttacggccgt
     3061 cgtttttctg acggtttccg ttttgttgtt ggtcacggtt attgccctta ttttgcttgc
     3121 gtcgttctgc ctgctcttta gcacgggctt cacgctcagc cttgaaatta cgactttgcg
     3181 gtttcactgc tactttttct gttttcggag caactggctc agccggtttt gcctcttcct
     3241 tagctacagc tggtttagct ggctcagatt tttcggcttt cttttctgca cttacttttg
     3301 gtgctgcagg ttttgcttct actttcggag cagctgcagg cttaaagctg gcagcaattt
     3361 ttgcagcgac agcttcttcc acacttgatg agtggctttt cacatccaag cccaactctt
     3421 ttgcacgcgc tacaacttct ttactttctt ttccaagttc ttttgcgatt tcgtacaatc
     3481 ttttcttaga caaatcatgt cctcctcttc tattccataa gagacctcat tttctttgta
     3541 aatccagcat ctgttacagc caaaaccttt ctcgatttcc cgactgctat gcttaattcc
     3601 agtgttgaaa acacggttac aatttctact tgataataat gacttttatc ttgaatcttc
     3661 ttggtcagat tgggtccagc atcatgagct agaaagacca acttggcctt gccgtcttga
     3721 atggccttga ccaccaattc ttcacccgat atgatgcgcc ctgctcgctg agcaagcccc
     3781 aagagattac ttatcttttg cttattcaag tcccaactct cttcttttca ctttgtgatc
     3841 cacataagcg atcaactcgt cataaaagct ttcttccact tccatgctaa agctgcggtt
     3901 aaagaccttc ttctttttcg cctctagggc ttctgcattg tctagtttga tataagcgcc
     3961 gcggccattg gccttgcccg taggatcaat aaagacttgt ccttccttgt tcttgacaat
     4021 gcggagcaaa tcacgcttat caatcacttc gttagacaca acagacttgc gcaaagggat
     4081 ttttcttgtt ttcatctttc cctcctctag cagcttttat tcttctacag tatcgttttc
     4141 tacttccaac tctactgaag cagcgtcttc catggcttca aattcgctag cagacttgat
     4201 atcgatacgg taaccagtca agtgagccgc caagcgcacg ttttgtccac gacgaccaat
     4261 ggcaagagaa agcttgttat ctggaacaac caccaaggca cgtttgctgt cgttttcatc
     4321 aaagataact tggtcaacct cagcaggagc gatggcattg tagataaatt cagctggatc
     4381 tgctacccac tcgataacat cgatattttc ttcgattggt accatgcggt catttttagc
     4441 atcgtaacga gctgggtgga atttgctagt aatcttcttg atattagcac caccacgtcc
     4501 aacgattgta ccgatagcat ccacgtttgg attgtggcta cgaacagcaa ccttcgtacg
     4561 gtcacctgct tcacgagcca cgctcatgat ttcaacagtt ccatcataaa cttctggaat
     4621 ttcttgctcc attaaacgtt tgatcatttc tggatgacta cggctaacaa agacgttcac
     4681 accacgaggg ttgtcttcaa ccttgtaaac ataaacttcg atacgatcat gagaagcaaa
     4741 aacttctcca ggaatttggt cttgttttga caattgggct tcgatgctac caaggttgac
     4801 atagataaag cggttgtcaa agcgttctac tgtaccagac atgatttctt gctcatgttc
     4861 tttgtaagta ttgtaagtga tggcacgtgt ttgcttgcgc attttttcca tgatggtttg
     4921 tttggcagac tgggctgcta cacgaccaaa ctcagctggt gcttcttcaa acttgatttt
     4981 gtctccaagt tcataagctg aattaatggc aagagcatct ttcaagctga tttccaaacg
     5041 gctatcaaat acttcatcaa caacttcacg gacagtataa actgtaaagt cacctgtttt
     5101 ttcgttgaag tcaatagcta cgctgtctga ctgaccatag cgtctgcgat aagcggaacg
     5161 aagcgactct actactgcgt cgatgatatc ttcttttttg attcccttgt cttcttccaa
     5221 aatgcggaag gcctctagca tttctttact catgttttct tcacttttga atcctcaaaa
     5281 gctatccttt ctttttctat aatttaactg ctaaacgtgc ttttgatact aaactgtatg
     5341 gaatttggac ggttttctta cgcgtcttgt ccatatattc catagtcaac ccgtcctctt
     5401 cgaaggccaa caaggttcct tcaaagacct tttgcttatc gatggcttgg tagagcccga
     5461 catggatgta ttttccaacc gctccagcga cggcatcctt ggttttcaaa gggcgttcca
     5521 aacctggact ggtaatttct aggaaatatt gttctgggaa gggatctggc ttgatggtgt
     5581 ctaggacagg actgataatt tctgtcaagt ctgccgtgtc gttcaaggta attccttcgg
     5641 gtttatctac aaaaatactg agaatcatgt cactgccaat ctttccatac tcgatatcca
     5701 cgagttcaaa aggagcttct atgacaggtt ctacaacttc tctgactaat tctacgattg
     5761 ttgcgattgc gtccacctcc tcataagcaa gaggcgaaga tatttccccg cctcactttt
     5821 catattctta ctatcagtat agcacgtttg ccaacttatg taaagcaaaa gcacttgtac
     5881 agcctaattt taggctatct cttaaaattc tgcctcaact cggtagataa cttgcccctt
     5941 gtttgagaat ttttgctcgt attctgtcat gacattgcct tcaaaatcac tggcatgcaa
     6001 atctaaccag acaccattga gtttcatgcc atattgagaa aagctcacta aactgtactc
     6061 aaacaagcca cggttatccg tcttgaaatg aatttctcca ttttcaggca agatacgttt
     6121 gaaggtatcc aagaaggtct tgtaggtcaa acgacgcttt tcatggcgtt ttttcggcca
     6181 tggatctgaa aagttcagat acaagcgatc aatctcaccg tcttcaaaat agtcagttaa
     6241 gtcagaacca tctacccaca agagcttgat gttaggcact ccaacttcaa gcaccttgtc
     6301 caaagcgtag ctcaaaacag acttttgaat atcaatcccg atatagttga tgtcagggtt
     6361 ttgcttggcc atacctgaaa caaaggcacc ctttccactt ccaacttcca catgaatggg
     6421 attatcattg ccaaacaagt cccgccattt tgccttggct tccaagggat tgaggaccac
     6481 atactgggga tttgcctcta gtaattctgt tgccccttta cgatttctaa ctctcatctt
     6541 ctctttccat acttgtctcg gaaatgacgc aaaccatgaa tctcccgatt gacattttct
     6601 aaatcttggt tcatataata cttggaaatc tgactcaaat aagacaattg accataccaa
     6661 tacaatttac ttaataccgt ttgattgtac ttgtaaccgt agtaggtcaa ccattccttc
     6721 cactgatgtt ctgaaatata atggcagagc atatgggcca catcaaacat gcgatcggtc
     6781 aagcgaaccg aatcccaatc tactaaataa atcaagccac tatctgtctc aatccaatta
     6841 ctatgtcgta catctccatg gacaatggtc gcatggtcct ctctaaatcc tggaatagtc
     6901 tgacgtaaat cagccatcac ttcactgata aaatgatttt tacgcaaagc atctggagcc
     6961 gtttcctgcc aagactggag taaatctaca ggtgtttcca tggcatagcc caaacgactc
     7021 aattgtgtca tcaacggacg tgagcgatga agacgggtta aaatattgac gatttgctta
     7081 cgattcatat catagggggt caatatcttg cctgtcaacc attcttgagc acacatatca
     7141 cgcccatctg ccaaacgacg actccataat aattgtggag caatttgttc tctagctaga
     7201 ccaggtagga ttggagaggt gttcattttt acaaagatgc gcttcccgtc aggatagcta
     7261 cccatataag ccttgccact tttcccaggt atgggagtca gtgttagctc attatcaccc
     7321 aaatccattt ctttcctccg tataaagttt ccttactatt ctactagttt ttggcctatt
     7381 cgtcaaccac tctaacccga ttctcaaaga aaagcctctg gattggcttg ggtatcatta
     7441 tagcttgaaa aaggtaagca ccatcttctg cttacctttt cataattttt ataaataata
     7501 taggagtggc caactgttgt aaaacatgtg gactagagta gaaacccata ggttttgact
     7561 ctttttataa gcaaagccca gcaacaaacc cccaagtaga taaaaaataa acgaagggac
     7621 attagaaggt ccatgcatga aagaaaagag agaggaagtt agcacaagcc ctaaccaaga
     7681 attgtcaaaa accgcacctt gaagaagtcc tctgaaaatg agttcctcct ggatggggcc
     7741 aaaaactgag gtagtcaaaa tcaaagacag ggaaattcct ctgctgactt ccgccaagtg
     7801 ttggacccct gagccagaag aaacagcgaa gaaatgaata taaaccagcg tctcaagtac
     7861 acttagaaga aagattgcaa tgaaaagcaa aagttgtttc ttgcttaaca tcccaaaaga
     7921 aatcatttca aactttctag cataagcaac actcagcgag gttacaagaa gacttacaag
     7981 aatcaatgca gattcatttt gaaaaaaacc cccatggtta atcgtataag gaactgtaat
     8041 gacaattatt agtactaaaa atccaaaaca caaggcatga aatttatcaa aaaactccat
     8101 aaaactatcc tcctcaccaa acagacttcc tacgcgtcat tctttagttc tagcctttcc
     8161 aaaacaaacc attttatact cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc
     8221 cgtactcaag tacagcctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
     8281 tagtaaccaa aatccgacca catagccagc ccctatgaat atagccatta aagctagcat
     8341 ggaatttagg aaattaaaaa ccaccgcaga tacaaaggtt agcacaaaaa cattaaaagc
     8401 aatggtgtca gaagccaaga ctagaatata gggtgtcaac cgatctaaag ttttggaatc
     8461 taggaaaaat aagtgtttat acatgataat ctcctctatg gctgaaaagc aagccttttg
     8521 tttttttacc ccaagaccct atgtaaaaaa gtcagcaaaa atggtaatgt tgcgaaaata
     8581 ttattgatca catgcaccgc ataggaagga taaatgctct tggtatagcg ggtcaaacta
     8641 gcaaagatga tgccagatgt tgcaaagatg aagatatcta acaaactagg caggcttgaa
     8701 aaatgaggaa gagcaaataa aattgacgga agaagcaaat caaggccaaa tctcgaatgc
     8761 ttaaaaaagg cgtgttgcag taatcctcta tagattagct cttccataag tggagtcaga
     8821 aagaacaaag ttatatagat acctagctca gcaaagttgg tcccactata accgatcaat
     8881 acagcccaac cttcagcagt tgactgaaca tgtttagctg tctgaacgtt aaaagagatc
     8941 tggagcacta ccactaatac tgtcaaaatc gaataccaaa gccatttttt tcttggaatg
     9001 tgaaagagat aaccatgacc tgtcttaacc agaatccaaa tcatgactcc gctaaatagc
     9061 aaactcaaga aattttgaat ccagacgaaa ttgccaatct gggaagaaaa ttgccaataa
     9121 ttttgaataa taagcgtcat ctgagaaaga ccaaatacga aaaataagta agagaaaact
     9181 gcacttattt tgaatagaag ttgatacttt ttcatagaaa tcctccctac tatgacctca
     9241 ccttgtcagg ctctactgct gtaagattaa gaagacagtt tgtttttttt aaggctaacc
     9301 tgactactag ataatagata cattaaggca ttaaagacaa tgaaaatatg tccatagaat
     9361 aaaatcaacc tcgcatccaa accaagataa agtttgatta tcaaaaagat gagcaaaaga
     9421 atttgaaacc ataaggtttt tccaaaaata aatttaaagc gatttcgaat atctacttcc
     9481 ttgattttta ccgccacccc tttattagca agaaggaaaa ctcctgcttc aaacaaacca
     9541 ctgtaaagaa caagccaccc aatagatacg atagagattt gtaaaaatgt ccctaaaaga
     9601 atatccaaca cactactcaa gaaaataaca aaaaataatc tgtatttcat attaaatacc
     9661 tccattcatt tatttcacta acaatttaat agagccttct actcaaatat cctgtcagaa
     9721 aaggatagaa agctactttt tataatactt caagccccac atgagcagaa gcgtgataaa
     9781 caagcagaga atacacctat ataagcgatt agttgttgat agaattctgt ttctgaaata
     9841 cctctataca aacaaatgac aaacataaaa tctgccaagc cgataaacat aagttgattg
     9901 gttctaggac taaccaaatc atcatttact tatatttaag agtatctctt ttatgttaat
     9961 gtatgttagc actgaaaagc aagacaggcc aataatattt aaaatgaaca gtaacggggt
    10021 taagtctcta aaaaaattat ctactgacac tacaagaaat actatacata ttatagtcga
    10081 aactatcttt ttcttatcca taattattta ctcctttcct aacaaatcca acttatcaat
    10141 caagagcgat ttttaacata atgtagcagc acccgttgca actttgacaa gtttagtata
    10201 tcattgtttt ttaaaatttt tcatccaaat cttgaattgt catcgaaacc tcctaaattg
    10261 caaaaacttc attagcccct actttttata atacttcaaa ccccaaatga gtagaagcat
    10321 gatgatcaag caaagaatag caccaatata agcgattagt tgttgataag attctcctgc
    10381 tgtgatacct ctatacaaac aaataataga cataaagcct gtcaagccga taaacatgag
    10441 ttgattggtt ctaggactaa ccaaatcatc atcttcaaac tctcttaatc tcatttccct
    10501 agtaagataa acagtaacca aaatagaagt caagttaata actactaaaa gaaattggaa
    10561 aactgacgaa atagaaatag ataagtagaa acaagcaaag gcaactgacc caggaacaat
    10621 ctcacaaaga atatgattct ttttaatcaa gaaaatcttt catttctttt ctttcttttt
    10681 aattaaagaa aaatagatca taaccgcaat catataggct atggtataaa atagattata
    10741 ccaaatactc tccctaagcg gataaagaaa gatggacatg atcagataca gaacgaaaat
    10801 aatcagtatt tttttcttca taagttttcc tcctaaatgt gcgatttatc ttagttgagc
    10861 aagaacattt acactgctag tatagcactt attttgacct tggatcactc aaatcataaa
    10921 tggtcatcaa aacctcttga attgtaaaaa ttaaaaaagc aagcatgaaa aacatacttt
    10981 cctctttata ttgtattgat accaacttgt ttgtagactt ttcatcctgc tatcacatat
    11041 cattttgaca ggcgaaacaa tattaaagaa actcccctgt aaattaagct agcaaataca
    11101 ggggagaaat ttatttttta gagagtacta tccgtatcct ttttggaaga ttttgaaaat
    11161 atttttctaa ttaagtcatc catataagga ccaaatatac caactactaa accaataata
    11221 aaacttttaa aatccataat taccaccaac atgttgctgc ataggctaca cctccaagta
    11281 tagctccacc tgcagcacca gttacaccta ttcctatagc aaatggtccc aatagaaatg
    11341 tcaaaccgtt gttgcacacc catcaattgc gccatatgca acccctgctg cacaactaat
    11401 ttttcttccc caatcaatat ctccaccttc aacgcaagca agcatttcat tatccataac
    11461 agaaaattgt gacatcattt ttgtattcat atagtatgtc acttttcagt tacggaacaa
    11521 gtttaatata aaaattatca aaaaaacata ggcaataaag agaaaaatta atttatcata
    11581 gattagaaat aatatgacaa aacaattcaa tgatgttaat tcaatagtct tttgtttttt
    11641 atcagagata cttatggata gataaataag ataggtttga aaagcgaaga gaataataaa
    11701 gaatatagcc ttcataaaat ttagctttca tttttatgat gtagcggtat aggctaaata
    11761 tccacaaacc actgctcctc caattcctcc tattgcagcg ccccatggtc ctagaatttt
    11821 cccatatttc actccacccg ctgcacaacc taaagcagca actacagctg ctcctccaac
    11881 acctgctttg gcaaaatctc cccaattgca tccgccacct tcaacgcaag caagcatttc
    11941 agtatccata acagaaaatt gtgacatcat ttttgtatcc atgacaaata ctcctttttt
    12001 aaaaaactaa aataaatcag aatagaatcc tcataatttt actataagtc ttaccaactt
    12061 agtcccaatt tatcaccaac catacctcct aagcatgtta atccaccccc aattgcacca
    12121 atgtgtgctc caacaaatgc accagcaagt ccagctactc ctaaagtggc caaacctgct
    12181 ccagttccac cagttataat tcccgtagtg actcctgtaa tcagtgcatt ttgacaatca
    12241 gtggagctat accccccttc aactttcgca agcatttcag tatccataac ctctaactgt
    12301 gacaacattt ttgtattcat gatgaatacc tcctttttat tttcaatttg ttaccaaagt
    12361 cttaaattca ataaacaaat agatttttta tagtatcttt ttgattttct taaaaaagta
    12421 tatacgtcta ctatcttctt aaaggtagca gtacctattt tttagtctaa gatttcaata
    12481 atcttgagta tctaaaatat cttaatttcg ttattctcct tgcaataaaa agttttacta
    12541 tactatttat taacttgcag aaagcaaaaa atattagtaa ataatagttt atagttaagt
    12601 tttttattcc taccaatcca tcaactaagt aaagcatcaa cgattacata aacgattgat
    12661 aatataatta aaattttgct aactatctta ttctcatgat tcttagataa ctttgatatt
    12721 ttgtaagtaa gtaaataaga cagtaaatta atagcgataa taatactata tttaagaatc
    12781 ataatcttac aaagaggaca taattcctga acctacacaa ataagtgttg ctgctccccc
    12841 agttatcgga ccagtcgcag cagctaatag tactgctcca atacaaccac cgattgcaga
    12901 tcctaaattg cctcttcctc cactaactat ttcgagttct tcattatcca taacagaaaa
    12961 ttgttccatc atttttgtat tcatgacaaa tactcctttt ttcttttttt atttttgtct
    13021 tgttgtaact ttgataagtt tagtatatca tcgtttttta aaatttttca tccagatctt
    13081 gaattgtcat cgaaacgtct tgaattaggt tttttatttc aagccacctc taaatgttta
    13141 aaaaaaataa tttctaatca cttttttacc attcaggaag ttttaatgac tattcaagat
    13201 ttcataaaat atgaacttag ttttatgaca taatagacct atccactata tgaaaggaat
    13261 tgccaatgac ttcttataaa cgtacatttg ttcctcaaat agatgcgaga gactgtggtg
    13321 tcgctgcctt agcctcgatt gctaaattct atggttcaga tttttctcta gctcacttga
    13381 gagaacttgc aaagaccaat aaagaaggga cgactgctct tggcattgta aaagccgctg
    13441 atgaaatggg ctttgaaaca agacctgttc aagcagataa aacgctcttt gacatgagtg
    13501 atgtccccta tccatttatc gttcacgtta acaaagaagg aaaactccaa cattactatg
    13561 ttgtctatca aacaaagaaa gactatctga ttattggtga tcctgaccct tctgtaaaaa
    13621 tcactaaaat gtcaaaagaa cgctttttct atgaatggac tggagtagct atttttctag
    13681 ctaccaaacc cagctatcaa ccccataaag ataaaaagaa tggtctacta agcaagcttc
    13741 cttcctctga ttttcaaaca aaaatctctc attgcttaca ttgttctctc aagcttattg
    13801 gtcactatta tcaatatagg tggttcttac tatctccaag gaatcttgga tgaatacatt
    13861 ccaaatcaga tgaaatcaac tttaggaatc atctcagttg gtctggttat cacctatatc
    13921 ctccaacaag tcatgagctt ctccagagat tatctcctaa ccgttctgag tcagagatta
    13981 agtattgatg tgattttatc ctatattcgc catatttttg aacttcccat gtctttcttt
    14041 gcgacacgtc gtacaggaga aatcatttca cgattcacag atgctaactc tattatagat
    14101 gccttggctt ctaccattct ttctcttttt ctggatgttt ctattctgat tcttgtagga
    14161 ggcgtcttac tggcacaaaa ccctaatctc ttccttcttt ctcttatttc cattcctata
    14221 tacatgttca tcatcttttc ttttatgaaa cctttcgaaa aaatgaacca tgatgtcatg
    14281 caaagtaatt ctatggttag ctctgccatt atcgaagata tcaacgggat tgaaactata
    14341 aagtcgctca cgagtgaaga aaatcactat caaaatatag acagcgaatt tgtagattat
    14401 ttggaaaaat cctttaagct cagtaaatat tctattttac aaacgagttt aaagcaggga
    14461 acaaaattag ttctgaatat ccttatccta tggtttggcg ctcaattagt catgtcgagt
    14521 aaaatttcta tcggtcagct gattaccttt aacacacttt tttcttactt tacaactcct
    14581 atggaaaata ttatcaacct ccaaaccaaa ctccaatctg cgaaggtcgc taataaccgt
    14641 ttgaacgaag tctatctagt cgaatctgaa tttcaagttc aagaaaaccc tgtccattca
    14701 cattttttga tgggcgatat tgaatttgat gacctttctt ataagtatgg ttttggacga
    14761 gataccttaa cagatattaa tctcacgatt aaacaaggag ataaggttag cctagttgga
    14821 gttagtggtt ctggtaaaac aactttagcc aaaatgattg tcaatttctt tgaaccctac
    14881 aaaggacata tttctatcaa tcatcaggat attaaaaaca ttgataaaaa agtcttgcgc
    14941 cgtcatatta attacctacc acaacaagcc tatatcttta atggctctat cttggaaaac
    15001 ttaactttgg gcggtaatca tatgattagc caagaagata ttctaagagc ttgtgaatta
    15061 gctgaaattc gtcaagacat tgaaagaatg cctatgggct atcaaactca gctctctgat
    15121 ggagctggtc tatcaggagg acaaaagcaa cgaatagccc tcgctcgtgc tcttttaact
    15181 aaagctcctg ttttaatact agacgaagct actagcggtc ttgatgtctt gactgagaaa
    15241 aaggttatag ataatcttat gtctctaact gataaaacca ttctctttgt agcccatcgt
    15301 ctcagtatag ccgaacgaac caaccgtgtc attgttcttg accaggggaa aatcattgaa
    15361 gttggtagtc accaagagtt aatgcaggcg caaggtttct accatcatct attcaataaa
    15421 taaggagaat gtcatgaatc ctaatctttt tagaagcgtc gagttttatc agagacgtta
    15481 ccataactat gcgacagtgt tcattatacc tctttcatta ctatttactt tcatcttgat
    15541 tttctccctt gttgccacaa aagaaattac tgttacttcc caaggagaaa tcgcccctac
    15601 aagtgtcatt gcctccattc agtcaaccag tgataatcct atcctagcta atcatttagt
    15661 ggcaaatcaa gtagttgaaa aaggggactt actcatcaaa tactctgaaa caatggaaga
    15721 aagtcagaaa actgccttag caactcaatt acaaagactt gagaagcaaa aagaaggact
    15781 tggaattttg aaacaaagct tagaaaaagc gactgatctt ttttctggcg aggatgaatt
    15841 tggctaccat aataccttta tgaattttac taaacaatcc catgatattg aactgggtat
    15901 cacaaagact aacaccgaag tttcaaatca agctaatctt tccaatagca gttcatcagc
    15961 tattgaacaa gaaattacaa aagttcaaca acaaattgga gaatatcaag agttgagaga
    16021 tgctatcata aataacagag cacgcttacc aactggcaat ccgcaccagt caattttgaa
    16081 tcgttatctt gtagcctcac aaggacaaac acaaggaact gcagaggagc catttttatc
    16141 tcaaattaat caaagtattg caggtcttga atcatctatc gcaagcctca aaattcagca
    16201 agctggtatc ggaagtgtag caacttatga taacagttta gcaaccaaaa ttgaagtact
    16261 ccgcactcag tttttacaga cagcctcaca gcaacaacta actgtggaga atcaattaac
    16321 agaattaaaa gtacaactag atcaagccac acagcgtttg gaaaacaata ccttaacctc
    16381 cccaagtaaa ggtatcgttc atctgaacag cgaatttgaa ggtaaaaata gaattccaac
    16441 tggtacagaa attgctcaaa tattccctgt catcacagat acaagagaag tactaatcac
    16501 ttactacgta tcttctgact atctacctct actagataaa ggacaaactg taagattaaa
    16561 actggagaag attggaaatc acggcaccac catcatcggc caacttcaga caattgatca
    16621 aactcctacc agaacagagc aaggaaatct ctttaaatta accgctcttg caaaactatc
    16681 taacgaggat agtaaactca tccaatatgg cttacaaggt cgcgtcacta gtgtaactac
    16741 aaagaaaaca tattttgatt atttcaaaga taaaatttta acccattctg attaattttc
    16801 agataacact ctataactat ttattatctt atcaaaaagg agaatcataa catggataag
    16861 aaacaaaacc taacttcatt tcaagaacta acaactaccg aactcaacca aattacaggt
    16921 ggaggattgt gggaagattt attatataac attaatagat atgctcatta catcacataa
    16981 gaacttcatc atccaataca actataaaaa aataagaccg agaaacaagt actctcggtc
    17041 ttatttttca tcattctgta tgtatcacag taagtacctg acgaaagact tgattttgac
    17101 aggtggtatt tagactggta ttaggatggc tttccacaat cttcatgacg gtatagagac
    17161 caactcctct ctcctcccct ttagaactgg ctccaaagga gaagatttca gaaatatcga
    17221 tgccctcttc tttgatggag ttttcgatga taaaggtctc ctgtgctcca ttttttaaaa
    17281 aggcgattga aacatgaggt tgactagctt ccacactggc ttcaatagca ttgtcacaaa
    17341 ggatagacac aatggttaga aaatcaagta gactcatccc ctcgacctga atctcctcag
    17401 gaacttcgac attaaagaca atgttcttat ctctggcttt taaaaatttc cctgctagaa
    17461 gacttttgag ggctttatca cgaatattta ccaatctgcc caggtcatat ttattgttct
    17521 gcaatttctg actggaatcc tttaagacgg agccatagac ctcttttatc tgctccatat
    17581 cctcctcttc aatgcccaga cgtaagctag tcaagaggtt ggtataatca tgacgaaagc
    17641 tccgtacttc cttgtaaagc tcctctatat gccgactata gcgttccata tctctatagc
    17701 gcagggcctg ctcttgttcc aatctctcat agagtttttc cttcaaatag gtatccaatt
    17761 tcttgataac ccccataaaa aagagtaggt aaaagactag gatgagatgg cgaacagtct
    17821 ttgattgaat actttgttca tattcaaaaa aagacagact ttccatgact agatagtagc
    17881 cacccattat ccagttaatc tgagtcaggg acttttgaaa ggctttatcg agaatctcct
    17941 ttctcaagct agtaaaatcg tagtccaacc atttcaaaaa agctagagaa atgaagaaat
    18001 tgaaaattat tatacataac ccagtaaatg agtagccatc atatacttgc ccttgtccca
    18061 aaaatggaag cacaaaatag gagactcctc tataaaagag attcaccaat atcattggaa
    18121 agagaccata aaagaaaagg agttttttag gaagccctct caataataag aaagataagc
    18181 ctatgccgta caagggttcc ataaaataag ataggtaaac atttcctact atatagctaa
    18241 tcatcacaaa aacaaaggcc aacagtatct taaaaagaaa ggccttaaaa atcctctcga
    18301 aagtaagatc aattccatcc accttaaaga agatgacaat ttctagtcca ttagtaacaa
    18361 gtgtatacaa caatatccaa gcaatgttca taaattctcc tagctcagtg taatttattg
    18421 atggcctcag acacttccct gaccttataa cgggcgatta gacaacttcc accattggga
    18481 aagaaaagca gtttttcttt cttatccaaa tgcaccacat ttgcaggatt gatgagaaaa
    18541 gagcggtggc actgcaagag acggggctcc tgcttgaaaa cctcctctaa actcgctgta
    18601 aattccagcc tgtctgtctt ggtatagaga ataacacgat gggctctggg cgacgtttcg
    18661 agatagtaaa cctctttaaa aggatactga aattgggcaa attttgattt aaagtaaaag
    18721 caatcttccg ccagactttt actatcttga ctattggcat agaggagggc tgtctcgatc
    18781 cgagattcaa actcctctgc tgacaaggcc ttatcaatgt agtccaaagc agacacttgg
    18841 tagcgaaaag acaggggcat aaactccgag tgagtcgtca caaagacaat cagggcataa
    18901 ggatcccgat cccgaatctt tctagccact tccagtccct tcatctcttc atttcgaatc
    18961 tcaatatcca aaaagaatag ctgatgggcc cccttctcat gcacttcagc cagcagttgg
    19021 tccggcttgc caaatacctc aaaagagcta ggaatgatat gatgtgcttt caaaagtttc
    19081 tcaatcgtcg tttcaattct agtctattgg gaaaaatcat cctctaaaac aaatattctc
    19141 atcttcttac tctccctctt tcaaccattt ttgacgaatt tctctatagc gacgtctggc
    19201 atacttgaca gcgtattgac cttcttctcc gagaaaaagg attctttctt gaaaatcaat
    19261 cgattttaat ttatcaagat taaccaaaca atttcgatga catctcatca aggtttcccc
    19321 acattggttc tcaagattac ttaaattttg aagcatatta aaactagctt cttctgtaac
    19381 aatctgtacg gtatgggctt tagttggatg tgtttggata tagtagatat cgtcgatgtt
    19441 tactttatag actgttttct gcgtctgaat aatcatatat ttcattggat tctcctccta
    19501 agaaaagaat aacagaaaag gtctagccca agcacaccca atcctaaaca gtctttgaaa
    19561 actcctaaaa ggattatttt ctacaacatc atgatgagct tacaactatt ttatcataaa
    19621 attcactcag cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag
    19681 aattaaaagt gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc
    19741 agattaaagg aggcattccc atgacagata cagaccccat caaaagagct catactttga
    19801 ttactgactt aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct
    19861 ttcaggagca attaaactct attcttggtt ttctagccaa ggctgaaaca gtggataatc
    19921 gattcttgat tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg
    19981 ctcttgatcc agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg
    20041 accaagtcaa gaccaagtta atactcaatg aaaatcaaag agcaaactag gaagctagcc
    20101 gcaagctgta cttgagtacg gtaagacgac gctgacgtgg tttgaagaga ttttcgaaga
    20161 gtataagtct ctacggacca accattatcc tgtagtaaca aaaaagaagt tgagacagac
    20221 tgaactcaac ttctttttta gtgtcgtatc agcaatgtta gtcctgcatc tgacgtttta
    20281 cctgataagg caaatacaag actagtaaaa ccaaaccagc tcctagtaag gctagaaggg
    20341 ctagtttttc ttggaaggta atcaacccaa caactaattc cacaagtaaa acaaaactgg
    20401 tcaatcctcg aactacctcc tgtaagcctt tttccttttg ccccttgtcc agcggaaaga
    20461 gttgggtcaa atactggtag tcaaaggcat gatagagggc cagcaactgg aagagcaaga
    20521 ggtagttaaa gagaactacc actgctgtcg caatccaagc ttgctcgata aaaacctgcg
    20581 ccagtacgga aagtaagaga agacgaagac tgagagcaaa gaggtcgcca tttcgcagat
    20641 aagaacgcag atagagattt tgccaaatct tcccaggcac cttctgaaca gcctttaaga
    20701 taaagtccag ataggcacga cgcttaacgc tgtttgaaat tcccttgacc tgcgtaaaga
    20761 gggcaaagaa acgaagcaag acttgcttac gcttgctttc ttgggaaatg acatagtccc
    20821 aatccagtcc agtttcagtg aaaaatttgc tggccttttg acgaaagtgg aaatattttc
    20881 ctacccccaa taaaagcaca tagagcagaa aaactggcaa gccataaccc attgctaaaa
    20941 ataaaggcgc aaataacagc aagaaaaggg tctgtacaaa gagccaaaag actagggaaa
    21001 tgccagtttg acgcttgaga tggagcttga tttcctcttc tccaactaag agaaagagct
    21061 tgtctggagc ctccatatag gtggcaattc ctccccaaag taaaagtaaa acagacgtaa
    21121 ttcctacaaa caaaaggata ggccaatgat tttcaggaaa atgttgtaag agttgactgt
    21181 actggtaggc tagaaagccc aacaggacaa gcaggaacaa gacaaagtgg tcattgagca
    21241 catagcgcag ataaccaaga cactccttac gaaaggcctg ctttctcttt aaaaacaagt
    21301 ctttcatagg tcctcctctt ttgtcagagc caagtaaata tcattcaaac tagcctcagg
    21361 catatcaaag gcttcgcgta gttgcaggag attgcctttg gcacgcacct ctcccttgtg
    21421 aagaatgaca aaggcatcac acatcttctc cgccgaatcc agcacgtggg tactcatgag
    21481 aatagacttg cccttttgct tctccacttc caaaagctga atcaaatcag aaatagccag
    21541 cggatcaaga ccaaggaaag gctcatccac gatgaaaaga cttggatccg ccacaaaagc
    21601 acagataatc atgaccttct gcttcatccc ttttgaaaaa tgaacaggga accagtctaa
    21661 tttctgttcc aaacggaaca tttttaacaa gggctctact cgttcgaaag ccactttttg
    21721 ctcaataccg taagccatag caaccgtttc gatatgctct ctgagggtca attcctcata
    21781 cagactaggc gtctcagaaa tgtagccaat ctgcttgcgg tagctagtcg catctccttg
    21841 cagagtcagg ccattgatat tgatggagcc actataaggt gccaacagac cgataatctc
    21901 attgatcgtc gttgatttcc cagcaccatt gagaccaatc aaaccgacca actgcccact
    21961 ttcaacagta aaggacacat ctttcaaaac aggaacatga acatagccac ctgtcaggtt
    22021 tttaatttct aacatatttt ctccaaatct ggtataatgt agctatatta tatcaaaatt
    22081 cagtacagta gaggtagatt ttatgtcaga ttgcattttt tgtaaaatca tcgcagggga
    22141 aattcctgct tcaaaagtat atgaagatga gcaggtcctt gcctttcttg atatctctca
    22201 agtaacacta ggacacacct tggtcgtgcc aaaagaacac tatcgcaatc ttttggagat
    22261 ggatgctacg agcgccagcc aactctttgc ccaagtacca aaagtagctc aaaaagtcat
    22321 gaaagtcact aaggctgctg gtatgaatat catttccaac tgtgaagaag tcgctggtca
    22381 aacagttttt catactcacg ttcaccttgt acctcgctac agtgctgacg atgacctcaa
    22441 gattgatttt atcgcccacg aaccagactt tgacaaactt gctcaagtcg ctgaaaccat
    22501 caaaaacgct taaggagttg ctatgaaatt atccaaccta ctgctatttg caggagctgc
    22561 agccggaagt tatctggtca caaaaaatcg ccaaaccatc acagatgaag tcttgaatac
    22621 cactgaccgc gttcaagcta tcaaggacga tgtggatatt atccaaaaca gcctgcaaat
    22681 cattgaccag caaaaagaac ttatcaagga ataccaagaa gacttgactt acaagtttaa
    22741 ggtcttggaa aaggatatcc agactagact agctgtgata aaagaaatgc agggaactga
    22801 agataagtaa aaagagcccg acggctcttt tttaaaacgt aaacaagtta tagtgaattg
    22861 aatttagaat agcacatcat agtttctaaa gcattgttag agattacttt aaaatcctct
    22921 tatcaatttg ttcatattct atttcaatct actatatata tcccatcaac tatgcatcat
    22981 aatttaggta actcatactc aataaaaatc aaaaagcaaa ctagaaagct aggcacagac
    23041 tgctcaaaac accgttttaa ggttgtggat agaactgacg aagtcagtaa ccatacccat
    23101 gacaagccga cgctgacgtg gtttgaagaa atttttgaag agtatcattc accatttcac
    23161 aactaaatcg aagactttct gccgtaatga aaacacctga aacagcttgg tttcggctat
    23221 ccggaaactt taagacttag gttcaaagtt taggtatgga acttcgaagg aggtcgctac
    23281 cgtccgtcat tacttaggaa agtcttaaaa aatctataaa gtaaaaagag cccgacagct
    23341 ctttttgttt atactcaatg aaaatcaaaa agcaaactag gaaactagcc acgggctgct
    23401 caaaacactg ttttgaggtt gtagatacga ctgacgaagt cagtcacata tatacggcaa
    23461 ggcaacgctg acgtggtttg aagagatttt cgaagagtat tattctccat caaaggcatc
    23521 tttaatatgg tcaaagaagc cttttttctt tggatttact ttcaagtcac cagcagccgc
    23581 gaattctttc aaggctactt tttggcggtc gttcaagcct gtcggtgtta cgacattaac
    23641 agtaacgtat tggtcaccaa ctgcaccgcc acgaaggctc ggtgccccct tactacgtag
    23701 gcggaacttc ttaccagtct gagttccctc tggaataacc aattcaacat caccgtgaac
    23761 agttggaata tctactgtat caccaagagc cgcttggaca aagttgaggt tgagattgta
    23821 gaagatagtc gttccttcac gttcaaactt gtcgctagct tccacagaaa ctactacata
    23881 caagtcacca taaggtccac cgttaaagcc tgcttcacct tgaccagcga ggcgaatttg
    23941 ttgacctgtt tccacaccag cagggatttt cacatgtacg ctatgagctt gtttctcatg
    24001 acctgttcca tgacaggttg tacatggata tttgatttct tttcctcgac cgtgacagac
    24061 atcacaggtt acttggcgac gcatcatacc aagaggagtc tgcgtatcga cgttaatgac
    24121 accagcgcca tgacagcgtc cacaagtgac tggacttgtc cctggcttag caccagatcc
    24181 attacatgta cgacagccag cttcacgatg atacttaact tccttctcag ttccgaagat
    24241 agcttcttca aaggtcaaat tgacacgata ctggagatca tctccttggc gaggagcgtt
    24301 tggattgcgc gaagaaccgc ctccgccgaa gaaacttgag aaaatatcct caaaaccacc
    24361 gaagccacct gccccattga aaccgccgaa accaccagct ccaccaaaac caccattggc
    24421 gcctgcagca ccatactggt cataggcagc acgtttttgg tcgtcactca aagtctcata
    24481 ggcttcttga acttccttgt acttgtcctc agcaccaggc tccttgttga tatctgggtg
    24541 atattttttg gaaagcttac gataagcctt tttgatttcg tctgccgaag cgtttttgga
    24601 tacccccaga cgatcataaa attcagtatt gttcataatt caagatacaa agggcgtcaa
    24661 acggacacag ccatctagga gttttgacga cggacgctta cgtcctagaa aaaactatct
    24721 ttttggcaca gtccatagcc cgtgttcagt tacgaacacc cttattcctt tctattgatg
    24781 ttctgaatcg aaccaccatt taggtcgggt tcaattcctt tcttttatat ttagtaagaa
    24841 attctgaaac ccgtgttcag ttacgaacac tctttgttcc tttctattga tgagacaaaa
    24901 cccaaacaaa tgagattccg tcccttttta tcgaaaaaca gaggctgggt tgcaacctct
    24961 gcaaataagt aaattaactt tattttgatt ttaaatatcc tataagtaca gaaatattta
    25021 tatatttttg tctcttctta gtatttgcct atataattcc aattgatcat aagaaataca
    25081 actactgtcc aaatcatacc aattatgaac ggtattacaa atttatatgt ttgtatttct
    25141 aatgatatca tagctgatac catcgctgat acaaatgcag acaacaatac tgaagtaaca
    25201 aaattcctta ccgcttcatc atttttgaca caactatcat gctcagctaa tataatttct
    25261 ctatatatag tcaaacattc tactggaata ttatctataa aaccttttgc tgcttcatag
    25321 ttgtcagctt gattacaaaa atcattataa acttttcgtg tattttttag atactcatca
    25381 tccaatacac tcatcttatt tttccgtaaa ctctccgtct acgacgtcat cgcctgcgtt
    25441 tcctgttgct tgtgcgcctt ctgctccttc ttgagcttgt tgcgctgctg cggcttgttc
    25501 gtagagtttc acagcaaggc cttgagcttt ttcgttcaac gcttcaagtt tcgctttcat
    25561 gtcgtccaag ttgttgtctt cttgagcttt cttaaggtca tcaagggcag cttgggcagc
    25621 gtcacgttct gcgtcgaagc ctttaccttc agtttccttg attgtctttt cagtcgcaaa
    25681 gattgcttgg tctacttcat tacgaaggtc tacttcttct ttacgtttct tatcggcttc
    25741 agcgtttgct tctgcatctt tcatcatgcg gtcgatttct tcgtcagtca aacctgagtt
    25801 cgattggatg acaatagttt gttctttttg agttccaagg tctttggcct taacagacac
    25861 gataccgttc ttgtcgatgt caaatgttac ttcgatttga ggaattccac gaggtgcagc
    25921 tgggatatca gtcaattgga agcgtccaag agtcttgtta tctgctgcca ttgggcgttc
    25981 accttgaaga acgtggatat caacggctgg ttggttgtct gctgctgttg agaagacttg
    26041 tgatttagat gttgggattg tagtgttgcg atcgataagt tttgtaaata ctccacccat
    26101 tgtttcgata ccaagtgaca atggcgttac atcaagaagg acaacgtcct tgacatcacc
    26161 agtaatcaca ccaccttgga tagccgcacc catagcaact acttcatcag ggtttactga
    26221 tttgtttggt tctttaccag tttcagcttt aacagcttca acaacggcag ggatacgagt
    26281 tgaaccacca acaaggataa cttcgtcaat ttctgacaag ctcaaacctg catctgaaag
    26341 ggcttgacga actggaactt ttgtacgttc aacaaggtca cgagtcaaat cgtcaaattt
    26401 cgcacgagtc aaagtcattt ccaagtgaag aggtccagcc tcacctgcag tgataaatgg
    26461 caagctgatt tgtgttgaag ttacaccaga aaggtctttc ttcgcttttt cagccgcatc
    26521 tttcaaacgt tgcattgcca tcttgtcagt agacaagtcg ataccgtttt ctttcttgaa
    26581 ttctgctacc aagtggtcaa tgattttttg gtcaaagtcg tcaccaccaa gtttgttgtc
    26641 ccctgcagtt gacaatacgt cgaagacacc gtcacccaat tcaaggatag agacgtcgaa
    26701 tgtaccacca ccaaggtcaa ataccaagat tttttcttct ttgtcagtct tgtccaaacc
    26761 ataagcaaga gctgctgcag ttggttcgtt aacaatacgt tctacttcaa gaccagcaat
    26821 tttaccagcg tcttttgttg cttgacgttg agcgtcgttg aagtaagccg gaactgtgat
    26881 aacagctttg gttactttct caccaaggta gtcttcagcg tagcctttca agtattgaag
    26941 gatcatagct gagatttctt gtggagtgta ttcttttcca tttgcagaaa ctttttcaga
    27001 agttcccatc ttagatttga tagagataac tgtatctggg tttgtgactg cttgacgttt
    27061 tgcagcatca ccaacgatga tttctccgtt tttgaatgag actacagatg gagttgtgcg
    27121 gttcccttct gggtttgcga tgattttgct ttcagttcct tcaagaactg caactgctga
    27181 gtttgttgta cctaagtcaa taccgataat tttagacatg tgtttttctc cttgttagtt
    27241 aattttctat ttttcttttt gatactcttc ttaagtggcg acgccgtcag agcttgactt
    27301 cgtcaatctc tttgactaaa ctttgagcct aaggtctcaa aatttgcgca aatagcacca
    27361 cggcgaagag tatcgtcttt ggttcgcttc gctcactatt acaaagccaa acatatttta
    27421 tctttcttca tagtttattg tcgtttcgga caagttttct attatgtaaa ttgcgacacg
    27481 aggagtcgaa atcgatttta tttcgacgac gagttagtaa ggaagctagg caaacgtcat
    27541 agcgattgcc gttttctgac gagttgcttt agctaccgtc agaattgccg aatcgaacac
    27601 gccctaagct ctgagtaaaa aagatagatt ttcttgtgtt catcgaacac ttcgtcaatc
    27661 tcctattttt actgcgagct ttttacgagc tttgtatctt agttatacac cactaccatt
    27721 gctgggcgta ggatgcggtc atggagtttg tagccttttt gaaagacttg ggcgatggta
    27781 tctactgggt gttcatcgtc tgctgggaga gtttggatgg ccatatggta gttatggtca
    27841 aattcgccat ctgctgcgat ttcttcaatt ccttcttctt tcaaagcgtg aatcaagctt
    27901 tcttgcacca tcgccaagcc cttcttcaca tcatctgtca aaccttcaac tgcaagtgca
    27961 cgctcaaggt tgtcaagaga tggtaagatt gcttttgcca agtcctggct acgataacgt
    28021 tgcaagtttt gacgttcttc attggcacgg cgttggatat tttgcatttc tgcatgagcg
    28081 cgaagatatt tgttttcgaa ctcatctgca cgttcatttg ccaagtccaa ctcagacttt
    28141 tcaggagttg tttcttcagc tgtttccaca acttcctctt cttgaacttc ttctacttct
    28201 tcatttttta tatcttgggc catgttcgcc tcctttaatg atttcaatct taatgtactt
    28261 cgtaatgatt actgctgagg tagcggtaaa aatctgtcaa cttcatggtc aaaacacggt
    28321 tgaccacatt gacttgattg attagctgtt ggtaatccag attaactgga ccgataatgg
    28381 ctagaattcc aactccccga taaggaatga ggaacttact actaatcacc gctaggtcag
    28441 ctaaacagga ctcttgaccg tctgcaacac gaacattttg catctgatcc tcacgcaacc
    28501 cctcacgaat ctccaaggcc accttttgcg gttggtcaaa gaactgatag gctgctagat
    28561 tggcaaaatt caagagatgg accttgcccg ccatcacaat gttttcgttg aacatttcct
    28621 taaagatgtg ttcaaagaga tcgatgacat tatccgttgt tgtaaagtaa cgctggataa
    28681 tctgcggaat ctccgtccga atcttgtagt gaatatctaa aacggtgtga ccgaggaaac
    28741 gttcctgaat gatgctcttc agtttcagca aatcctcctg caagaagttc cttggaatca
    28801 gaaactgact agtaaccgtt cgcgactcgt ctagggtaaa taccgccaag gctgtatgtt
    28861 gccccaaaac aacgatatca aaggctgtca aacgttgcct gctcggctca acatccagtg
    28921 ccactaccgt acagccactc aggtctgtta gcaagttagc agcctcttgc agaatatcct
    28981 ccaatttgaa gaattcctga tcaaaggctt tgacaatctc atatacctca ttttcagcca
    29041 gtcggtcaaa atccagtgag tgtttcacat agtactgaaa accagcaaca cttggcatcc
    29101 gaccgcttga agtatgagcc ttctcaagca acccttgttt ttctagttcc gccatgtcat
    29161 tacgaatggt tgcactgcta gagttaatag actcttgcaa ggtttttgat ccgacaggtt
    29221 cgtgcgtttt ggtaaagatg tcaataatca gatttaaaat atcctgctga cgctctgtaa
    29281 ccatctcatc actcctctct gtctgatcaa ttagcactca atgcatccaa gtgctaactc
    29341 atgattctat tatacaccct tagaagagaa tgtcaagaca aaaactcaaa aaattagcac
    29401 tcttttatca agagtgctaa aaatcaatct gaagacctag aaccttgcta ttcatctact
    29461 cggtatttcc tttttagtct gaaataacca tgaattagat aagaaatcag aaaagcatat
    29521 aaaacaaaaa ataagaaaga aagacataga aagatcctct ctaaacaaac tcatacaaac
    29581 aacaagacca cctaagaaag tagctgtaca ggaacgaagt ctagatcgca taacttccca
    29641 tttgagcccc ttactcatat agacttgatc ctctggaagt aaatcagaag acgatttacg
    29701 aagaatcgaa caagaacctg ctcctatcaa ttcccaacct ctatctctaa aatcttgcag
    29761 ttcacgctta tgctctttaa gaaatctaga atcatagata cgatagataa catcgtccgg
    29821 ttgaccttgc tcaaaataga acaaaccaaa acgactcgtt ctatacctcc aacctttcaa
    29881 atgcatctca tgtaaatatt cttctttctt gtccaaatca acaatggtga aaatccgaaa
    29941 ttctactctg ctattcattg tcttacccca aaattagaaa acatgcctgg cgttatttat
    30001 tagataattc tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcgcaa
    30061 aaactatcaa taaaacccaa tctattatga aaatcaaaaa cactttccaa ctgaaagaac
    30121 tacctccagt aacaaacttt gagaaaaacg gtagtagagc taaaaagaga agtaaaatag
    30181 gaagcatccg cattgttaaa atccgtttgg cataaaaaaa tctttattta aactaaaata
    30241 ttatagcaaa atttacgcca gtttttgaac ggctgatgta gatattttat actttcaaaa
    30301 tgtttaaatg tgattattta tttttgaaaa atagctcacc agcccgactg aaagtgctta
    30361 tagaatgata ataagtcgcc tgccgaaaac agcggaaaat agcggtgtta tgcggagata
    30421 atctgacgcg atgcgaaagt atattgcata cttattttca acaatttagc agagtatttt
    30481 tataagtgtg atataataga agtataattt gttctgatag tttattttat ggagaagtag
    30541 atttttagaa tgcggagggt tcaatatggt tgagtttata aagtctaaga aagaaatgag
    30601 tgaggaggat attaaagcaa atttcatcac tcctgctatt gtatccaaag gatggaaaaa
    30661 tggtgagcat atcgcttacg aagaatactt cactgatggt cgaattgaag ttagaggaga
    30721 taaggctcgt cgtaaagaag gaaaaaaatc agactattca ctgtattacc aatttggaac
    30781 tcgaattgca attgttgagg caaaggataa taaacacagc gttcgagcag gattacaaca
    30841 agctattgaa tatggagaga ttttagatgt tccatttgtt tattcttcga atggtgatgg
    30901 ctttattgaa cacgaccgta tcacgagaga agaacgtgag ctggagttag acgaattccc
    30961 tactcgtgaa gaattatttt ctcgtatgac gaaggaaaaa ggattgacgt acgaaattac
    31021 agaagctatc tcaactccat actatacaga cgccttctca atgaaaacgc cacgctatta
    31081 tcagcaaata gctatcaacc gtactattga aacagttgcc agaggacaaa aacgagtaat
    31141 gtttgtgatg gcaacaggaa cggggaaaac gttcatggct tttcaaatta ttcatcgcct
    31201 tcgaaaagct ggtttggcta aacgagtttt attcttagca gatagaaaca tcttagtaga
    31261 ccaaacgatg gctgaagact ttaggccatt cgaaaaggta atgacgaaaa ttacaccaaa
    31321 acttttgact gctcctgaaa aattaaattc ttttgaaatt tatctagggc tttatcagca
    31381 actaactggt gaagatggaa ctgaaacaca ttatcaaaaa tttgacaaag acttctttga
    31441 tttaatcgta attgatgaag cacaccgtgg ttcagctaag gaaaacagta actggcgtaa
    31501 ggtaattgat tatttcagtt ctgcgacaca gattgggatg accgctactc ctaaagaaac
    31561 caagaatgct tccaatacgg aatactttgg tgagccaatc tatacttata gtttaaaaca
    31621 gggaatcgag gatggttttt tggctccata tcgtgttatg agggttaatt tagatgtgga
    31681 tgtggatggt tatcgtccag aaactggaaa agttgatgct aacggacaat taatagaaga
    31741 taggtactac ggcaggaaag attttgataa aaccattgtc attgatgata gaacgcaaag
    31801 agttgccaag tttgtttctg attatatgaa gcaaaacaat gcacgatttg ataaaacaat
    31861 tgttttttgt gttgatattg accatgccga gcgaatgcgt gctgcacttg taaaagagaa
    31921 tctagactta gtccaagaag actatcgtta tgtcatgcaa gtaactggtg acaacgctga
    31981 aggaaaagct caactggata actttatgga tgtcaattct aattttcccg ctattgtaac
    32041 aacgtctaaa ttattaacga caggagttaa tgctaaaaca tgtcgtttga ttgttttaga
    32101 ctctaatatc caatccatga ctgaatttaa acaaattatt ggtcgtggca cacgtcttta
    32161 tcctcaaaag gggaaagaat tttttacgat tattgatttt cgaaatgtta ccaatttgtt
    32221 tgctgaccct gattttgatg gtgatccagt gaaggtgctg gaaacaggtg cgaaaacagt
    32281 cagtggttct acgcccggtt tcgtagatga ggaaggtgac ccagtagaaa aatatatcgt
    32341 tacagacaag caggttacca ttcttaattc tactgttcaa gtattggatg aaaacgggaa
    32401 actgattacc gaaagcctga ccgactacac tcgaaagaat atcttaggta gctacgccac
    32461 tttgaacgat tttatcacag tttggcatac ggcagataaa aagaagctta tcttagacga
    32521 actttataaa aaaggagttt atctagatgc tattcgagag tcggagggaa tatcagaaca
    32581 agaaatcgat gattttgatt tactcctaaa acttgcctat ggtcaaaaag aattaaccaa
    32641 aacggaacgt atcaataaac tcaaacaaag cggatattta tataaatata gtgaggaagc
    32701 gcgtgctgtt ttggaaattt tactgaacaa atacatggat aaaggtattg gagaactcga
    32761 aagcattgaa acattaaaac ttccagaatt tcagatatat ggtggaacct tcaaaatcat
    32821 caatacttat tttggagata aaaaacgata tttacaagca attaaagaat tggagcaaga
    32881 gctatttaca gtagcttaat gaaaggaaag tatgtcaatt acatcatttg taaaaagaat
    32941 tcaagatatc actcgaaacg atgctggtgt taatggtgat gctcaacgta ttgagcaaat
    33001 gtcttggtta ttattcttaa aaatttatga tagccgtgaa atggtttggg aattagaaga
    33061 agacgagtat gagtcaatta tcccagagga attaaaatgg cgaaattggg ctcatgctca
    33121 aaatggggaa cgggtattga caggcgatga attacttgat tttgtcaata acaagttatt
    33181 caaagagttg aaagagcttg aaataacttc aattatgcct attcgaaaaa cgattgttaa
    33241 atcagctttt gaagatgcga acaactatat gaaaaatggc gtcttgttac gccaagtcat
    33301 caatgttatt gatgaagttg atttcaatag ccctgaagat cgtcattcgt ttaatgatat
    33361 ttacgaaaaa attcttaaag atattcaaaa tgctgggaac tcaggagaat tttatacgcc
    33421 acgtgcagcg actgatttta ttgccgaagt tcttaaccca aaacttggag aatcaatggc
    33481 agaccttgct tgcggaacag gaggcttctt gacttcgact ctgaaccgtt taagtagtca
    33541 acgtaaaact agtgaagata ccaaaaaata taatacagct gtttttggta ttgaaaagaa
    33601 agcatttcct catcttttag cagttacaaa tctgtttctt cacgaaattg atgaccctaa
    33661 aattgttcat ggaaatactt tggagaaaaa tgttcgtgaa tatacggatg atgaaaaatt
    33721 tgacattatt atgatgaatc caccttttgg agggtcagaa ttagaaacaa taaaaaataa
    33781 ctttccagca gaattacgga gttctgaaac agctgattta tttatggctg tcattatgta
    33841 tcgtttgaaa gaaaatggtc gtgttggagt tattttacct gatggttttc tatttggtga
    33901 aggtgtaaaa actcgcttga aacaaaaact ggtagatgag ttcaacttgc atacgattat
    33961 taggttgcct catagtgtct ttgcaccgta tacaggaatc catacgaaca ttcttttctt
    34021 tgataaaaca aagaaaacag aagaaacttg gttttatcgt ttagatatgc cagatggtta
    34081 taaaaatttc tcgaaaacta agccgatgaa gtcagaacac ttcaatcctg ttcgtgactg
    34141 gtgggaaaat cgtgaagaga ttctggaagg taagttctac aaatctaaat catttacacc
    34201 tagtgaattg gctgagttga attataattt agaccagtgt ggttttccaa aagaggaaga
    34261 ggaaatctta aatccctttg agttgattca gaattatcaa gcggaaagag caactttaaa
    34321 tcataagatt gataatgtat tagctgatat tttgcagttg ttggaggaca aataatgaca
    34381 ccagaacaac ttaaagcaag tattctccaa agagcgatgg aagggaaatt agtgccgcaa
    34441 aatcccaatg acgaacctgc aagtgaatta ttaaagagaa ttaaagctga aaaagaaaaa
    34501 cttatcagtg aaggaaaaat caaacgagat aaaaaggaaa ctgagatatt tcgtggtgat
    34561 gatgggaaac attatgggaa gtttgctgat ggaagtactc aagaaattga tgttccttat
    34621 gatattcctg atacttggga gtgggtgagg ttttctacat tggttgaaat tgtcagaggt
    34681 ggctctccac gaccaatcaa agattatctt acttctgaag tagatggaat aaattggata
    34741 aaaataggtg atactgaaaa gggtgaaaag tatataaata atgttaaaga aaaaatcaaa
    34801 aaatcagggc ttaacaaaac tagatttgta aaaaaaggta catttttgtt aactaattct
    34861 atgagttttg gtagacctta tattttgaat gttgatggtg caatacacga tggatggttg
    34921 gctatttcga actatgaaaa ctcattaaat aaagattacc tattctatat tctttcatca
    34981 aatgtagttt attctcaatt tctatctcta attagtggag ctgttgtgaa aaacttgaat
    35041 agtgataaag ttgcttctat tcttatccct ctccccccac tatccgaaca acaacgcata
    35101 atagaagcaa tcgaatcagc tttagaaaaa gtagatgaat atgctgaaag ttataataga
    35161 ctagaacagc tagataaaga atttccagat aaactaaaaa aatctattct tcaatatgct
    35221 atgcaaggaa aattagttga acaagaccca aatgatgaat cagtcgaagt tttacttgaa
    35281 aaaatacgag cagaaaaaca aaaactcttt gaagaaggca agattaaaaa gaaagatttg
    35341 gacatttcta ttgtttccca ag
//
LOCUS       DAVMMR010000018        35196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMMR010000018 DAVMMR010000000
VERSION     DAVMMR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.018"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(381..1361)
                     /locus_tag="VYS74_000843"
     CDS             complement(381..1361)
                     /locus_tag="VYS74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV3788905.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1677..3692)
                     /gene="recG"
                     /locus_tag="VYS74_000844"
     CDS             complement(1677..3692)
                     /gene="recG"
                     /locus_tag="VYS74_000844"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV3788906.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIGQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWCMIALHLEKKEHLD"
     gene            complement(3711..4814)
                     /locus_tag="VYS74_000845"
     CDS             complement(3711..4814)
                     /locus_tag="VYS74_000845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV3788907.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4804..5172)
                     /locus_tag="VYS74_000846"
     CDS             complement(4804..5172)
                     /locus_tag="VYS74_000846"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV3788908.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5211..6242)
                     /locus_tag="VYS74_000847"
     CDS             complement(5211..6242)
                     /locus_tag="VYS74_000847"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3788909.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6244..7275)
                     /locus_tag="VYS74_000848"
     CDS             complement(6244..7275)
                     /locus_tag="VYS74_000848"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3788910.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7356..9869)
                     /gene="secA"
                     /locus_tag="VYS74_000849"
     CDS             complement(7356..9869)
                     /gene="secA"
                     /locus_tag="VYS74_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV3788911.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10015..10302)
                     /locus_tag="VYS74_000850"
     CDS             complement(10015..10302)
                     /locus_tag="VYS74_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788912.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10313..10963)
                     /locus_tag="VYS74_000851"
     CDS             complement(10313..10963)
                     /locus_tag="VYS74_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3788913.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10967..11608)
                     /locus_tag="VYS74_000852"
     CDS             complement(10967..11608)
                     /locus_tag="VYS74_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788914.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11601..12392)
                     /locus_tag="VYS74_000853"
     CDS             complement(11601..12392)
                     /locus_tag="VYS74_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788915.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12414..12806)
                     /locus_tag="VYS74_000854"
     CDS             complement(12414..12806)
                     /locus_tag="VYS74_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788916.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAVDMARQNWWDLSAAISGSWSPN"
     gene            13133..13984
                     /locus_tag="VYS74_000855"
     CDS             13133..13984
                     /locus_tag="VYS74_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3788917.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(14706..15041)
                     /locus_tag="VYS74_000856"
     CDS             complement(14706..15041)
                     /locus_tag="VYS74_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788918.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(15132..16442)
                     /gene="der"
                     /locus_tag="VYS74_000857"
     CDS             complement(15132..16442)
                     /gene="der"
                     /locus_tag="VYS74_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV3788919.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(16456..17169)
                     /locus_tag="VYS74_000858"
     CDS             complement(16456..17169)
                     /locus_tag="VYS74_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV3788920.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(17166..18062)
                     /gene="dnaI"
                     /locus_tag="VYS74_000859"
     CDS             complement(17166..18062)
                     /gene="dnaI"
                     /locus_tag="VYS74_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV3788921.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(18063..19232)
                     /locus_tag="VYS74_000860"
     CDS             complement(18063..19232)
                     /locus_tag="VYS74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV3788922.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEYPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(19233..19706)
                     /gene="nrdR"
                     /locus_tag="VYS74_000861"
     CDS             complement(19233..19706)
                     /gene="nrdR"
                     /locus_tag="VYS74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV3788923.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            19846..20211
                     /locus_tag="VYS74_000862"
     CDS             19846..20211
                     /locus_tag="VYS74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3788924.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            20216..20911
                     /locus_tag="VYS74_000863"
     CDS             20216..20911
                     /locus_tag="VYS74_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788925.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            20928..21695
                     /locus_tag="VYS74_000864"
     CDS             20928..21695
                     /locus_tag="VYS74_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788926.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(22048..23247)
                     /locus_tag="VYS74_000865"
     CDS             complement(22048..23247)
                     /locus_tag="VYS74_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788927.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(23244..24137)
                     /locus_tag="VYS74_000866"
     CDS             complement(23244..24137)
                     /locus_tag="VYS74_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3788928.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            24253..24333
                     /locus_tag="VYS74_000867"
                     /pseudo
     CDS             24253..24333
                     /locus_tag="VYS74_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            24818..25031
                     /locus_tag="VYS74_000868"
                     /pseudo
     CDS             24818..25031
                     /locus_tag="VYS74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            25446..26063
                     /locus_tag="VYS74_000869"
     CDS             25446..26063
                     /locus_tag="VYS74_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV3788929.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(26383..27270)
                     /locus_tag="VYS74_000870"
     CDS             complement(26383..27270)
                     /locus_tag="VYS74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3788930.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(27419..29356)
                     /locus_tag="VYS74_000871"
     CDS             complement(27419..29356)
                     /locus_tag="VYS74_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEV3788931.1"
                     /translation="MDYSKVAAEVIGAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            29732..31186
                     /locus_tag="VYS74_000872"
     CDS             29732..31186
                     /locus_tag="VYS74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3788932.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            31167..32132
                     /locus_tag="VYS74_000873"
     CDS             31167..32132
                     /locus_tag="VYS74_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3788933.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(32167..33441)
                     /locus_tag="VYS74_000874"
     CDS             complement(32167..33441)
                     /locus_tag="VYS74_000874"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV3788934.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(33441..34613)
                     /locus_tag="VYS74_000875"
     CDS             complement(33441..34613)
                     /locus_tag="VYS74_000875"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV3788935.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETKSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(34744..34992)
                     /locus_tag="VYS74_000876"
     CDS             complement(34744..34992)
                     /locus_tag="VYS74_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788936.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIDELSSN"
     gene            complement(35060..>35196)
                     /locus_tag="VYS74_000877"
     CDS             complement(35060..>35196)
                     /locus_tag="VYS74_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3788937.1"
                     /translation="SKVENIFAKVKTFKMISTTYRNHRKRFGLRMNLIVGIINHELGF
                     "
BASE COUNT        10422 a         7794 c         6526 g        10454 t
ORIGIN      
        1 aacatatata cggcgaggcg aagctgacgt ggtttaaaga gatttttgaa aagtattaac
       61 acctacagac aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc
      121 tttctgaaaa aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataaat
      181 ataatagatt gaaactagaa tagtacacat ttgcttctaa aacattgtta gaaatcgatt
      241 tgactgtcct gatcgttttg tcctattctt gtttcatttt actatatttc catgataaaa
      301 cgcatagtat caagtttttt taatcccctg atactatgcg tgtttgtaat ttttaagatt
      361 ttgtgcttag agtcgactcc ttattttaga tatttaaaag gaatctcact tccacagagc
      421 cagttgtaga cttggtcatt gacaaataca ttcatggctt cgtgagcata ctcaggcatg
      481 atgcgatagg ttttatcgca ggtcagacga ttataaatcg caaactgggt aatgggatag
      541 caaacatcgt cgtccaagcc cgtaatcatc ttaacctcac cttggatacg atgggcaaga
      601 tttttgacat cgatataggc aagggtcgcc atgatttcct cctctgtttc atggaagggg
      661 tcgtgaaact tgaaataacg gaaaagttcg tcgtaagcct cgctagtatt accaatctca
      721 atcacccgtc tgaagtctga caagaaggga taaatggcaa ctgttttctg aattcgagga
      781 ttgagcgctg ctgcaactag agctagagcc cctccttgtg aggcaccata gctagaaaga
      841 cgcttctcat caacctgaga cagactagca acaatttcga ccaactggta aatatccaga
      901 taaacatcct tataaaagag gtggtcccga ccttccatag caccacggat aatatgcccc
      961 ttcacggtat ttcctaaagg agaacgcaag ccgtcttgtg agtaacctga ctggccccgc
     1021 acatccatgg aaacaacacc gtaaccagct acggtgaagc ccagcatgtc ggcccagtcc
     1081 cagccacgtc ccatataacc atgaaaatgg aagattagtg gaaccttctc ctcactcttt
     1141 ggaagaacaa tgcgtgcata gacctttcct tccttgcttc cttcaaatgt taactcatag
     1201 cacttgactt gaggaatgtg gaaatctctt tcctccaagt ggtaggatgg aagcgtggaa
     1261 acatttttca cttccccatc ccagaaagca tcaaagtctt ccggaacctc atcccttcct
     1321 ctataggtct taatttcttc tagcaaagct ggatttttca tagcatgctc cttttatttt
     1381 gtaatcgtta ccacaactgt agcatatcta tgtaatcaat gcaagaaaga aggataaata
     1441 gaaagtgaat ttagattctt tacttttagg atgatgggct gaaagtagtt ttagaaacga
     1501 aaaagagcat ttcgctctct cttctgttta tactagtttt ttgcttcctt cttttggaaa
     1561 gtggtttggt aatttacttt aatcgttact gtcatatgag agtcctcgtt tctttttgat
     1621 gactttagta taagagccaa acctaaaaac tagcttaagt tttccttaga gaaagcttaa
     1681 tccagatgtt ctttcttttc cagatgaagg gcaatcatgc accactctgg atcttcttgc
     1741 caagcttcta tagaactaat gtagctagca acctttcttg cttcttctaa aatcggaaaa
     1801 tcttcgataa tatcagccac ttggaactct ggaagtcctg actgtctggt tccaaaaatc
     1861 tcaccagaac cacgcatttt caaatcttcc tccgcaagga caaatccatt ggtcgtttct
     1921 gtcatgatgc gcatgcggtc tttcccagaa tccgtcttgg gattggcaac gagaacagcg
     1981 taggactgct tgtccccccg accgacacga cctctaagct ggtgaagttg actgagaccg
     2041 aagcgatcgg catccatgat aatcatgaca gtcgcattgg gaacgttgac cccaacctca
     2101 ataaccgtcg tcgaaaccag aatatccgtc tttctctcct tgaaatcctg catgatctgg
     2161 tctttttcgt cactcttcat cctaccatgt agaagagcca cctctgcctt gcctgcaaaa
     2221 tgagtcgtca actcctctga taaggcaatg gcatttttca aatctagagc ttctgattct
     2281 tcaatcaaag gagagatgac ataggcttgg gaaccttttt gaatttcccc ctctaaccaa
     2341 gtcaagacct gaggtagttg ctcatgtttg atccagcgcg tcacaatagg cttccgacct
     2401 gctggcatct ggccgataat ggaaacatcc atatctccaa aggctgtgat ggcaagcgtc
     2461 cgtggaatgg gagtcgccgt catcatgagg acatctggat tgtcaccttt ttcccgtaaa
     2521 atacgccttt gccctacacc aaaacggtgc tgctcatcga taataatcaa accaagacga
     2581 gcatactcca ccccatcttg tatcagagcg tgagttccta taatcaaatc agcctcaccc
     2641 ttggcaatgg tctccaagac ttctctcttt tctgcagctt tcaaggaacc tgtcaagaga
     2701 gccagtttca aattgggaaa aaggttctgt aaactctcaa agtgttgctc tgcgaggatt
     2761 tctgttggta ccattagggc agcctgataa cctgctgtca ctgccgcaaa catggccaag
     2821 ccagcgacta ccgtttttcc actccccaca tccccttgta ggagacgatt catgtggtgg
     2881 tcggacttca tatcagttaa aatttcctgc aaactctttt cctgagcttg ggtcagggca
     2941 aaaggaagac ttactttaac tgctgtcact ttttcctgag accaattcag aaccagacca
     3001 cttccctgaa ctctattttc agacttgagc gtctgcaact gcatttggaa ataaaagagt
     3061 tcctcaaact tgatacggcg aagagcctgc ttgtattctg ccaaatcctt tggaaaatgc
     3121 atagctcgga ctgcctgaca acgggacatg agtttgtatt tgtctagtaa agactggggc
     3181 agattttctt ctatcaagag gtccagtccc tgatcaaaag ccgtcttgat gaccttaacc
     3241 agactggcct gactgattcc ctgagccaga cgatagacag gctggaggtc atcttctacc
     3301 tgagccagaa ccttcatccc agtcagacta gccttagcgc ggtcccattt tccaaagaca
     3361 gcaagggttg ctcccaactc tattttatca gccagatagg gctggttaaa gaaattcacc
     3421 gcaaaaacga cctctccctg cttgagacta aaacgcaggc gattgcgctt gaaaccataa
     3481 tactggacac tagcaggagt cactacctga ccagaaagaa ctgccttctc accgtcttcc
     3541 agctccagca cctgcttggt tttgaagtct tcataacgga aaggaaagta gagcaagaga
     3601 tcttgcaagt tttcaattcc tagtttggca tatttttctg ctgactttgg tcccacacca
     3661 ggcaagacat gcaagggttg atgtagattc atgctccact cctttctttt ctaataatat
     3721 tctctcggaa tacggtcgct gaggaggcaa accacctcat agttaatggt tacgcggtag
     3781 gtcgctacct gagttgcagt gatttcctta tccccattgg agccaatcaa ggttaccttg
     3841 gttccgagtg ggtaaggctt aggcaatcga atagtgattt ggtccatcga aaccctgccg
     3901 acaattgggc aagcttggcc atctaccaag acagagaaat tttgcatgtc tcttgtccat
     3961 ccatctgcat acccgattgg cacggtcgcg atgacttgct cgctatccgc ttgataagtt
     4021 gctccatagc ccatgcaagc tccagctgga actgtcttga catgaaccag agcagactcc
     4081 aaggtcaagg ccggtatcaa atcataaggc aaatccaaga ccgctccact tggattgagg
     4141 ccatacatgg catctcccat acgaaccgca ttgaaaatag tctctacatg ccaaagagtc
     4201 gttgcagaat tgctagcatg aaccagctct ggaacttcct tcatactagc taaaatagtt
     4261 ttaaaccgtt ctaactgggc attaaaatag tcatctgatt cctcatcagc agtagcaaag
     4321 tgggtaaaga ttccttcaac acaaacaccg tgttgttgga gcaaatcttg agcctgctca
     4381 acctcacttg cctctctaaa accaatccgt cccatccctg aatcaatctt gaggtggact
     4441 gtcaatccag ttaggtccac ttccttatct aagagtgctt gaatccactc cagtccagcc
     4501 actgtcaagg tgaagtcata ttctttagct agagcaacag cttcgatttc agaaactcct
     4561 aaaatgagga ttggcttgct gagtccagct tgtctgagtt caatggcttc atcgatattg
     4621 gaaacgcaaa agccatcaac atcatcttga attgccttgg caacggcaac agctccatga
     4681 ccataagcat tggccttgac cacagccaac ttgagcgttc cttgagggat atgagccccc
     4741 atttgctgaa tattttgtcg aatagctccc agatgaatca gagccttggt tggtctatgt
     4801 ggactagctt tcatgatttt cctccaaaat gacactggct gtcacaaact gatcggtgtg
     4861 gctgatagac agccaaatct ttcctgaaaa tggtgcctga ctaaaataag gcgccccacg
     4921 ttcattgttc aagacttcca aatcctgaaa accgagcttg ctaatgcccg ttcccatagc
     4981 cttggaaaag gcctccttag ccgaccagcg accagctaaa tattctattt gcctgcgtcc
     5041 tttgagactg gtgaagcgct ccatttcctg agcggtcagt acacgcttag caaatccttc
     5101 atgtcgtgta actgcgcttt ctatcgaagc caattcttcg atgtcaattc cgtgtccaac
     5161 tatcattctc atcaaaagga gttgagattc cccaactcca tccttttcac ttattttgtc
     5221 aaggtaacat aaatctcttc taccaaggcc tctgtatttt cccaacctag gcaagggtca
     5281 gtaatagagc aaccaaagat ctctggttgg ttttgacgac catctgctag gtaagattca
     5341 atcataaatc ctcgaaccgt ctttttaatt ttctcattcc aatcacgatt ctgcaaggtc
     5401 tggcgaacaa ttcgaatctg ctccatatat tgcttgcctg agttatcatg gttggtgtca
     5461 atgaggataa aaggattttc aagtcccatg gtttcatatc gttcaatggc ttggagtagg
     5521 ttttcatagt agtaattcgg catataattg ccatactcgt tgactgctcc acggaggata
     5581 acatgggcca aaggattacc tgatgtctca acttcctgcc catgataaag gaaggtttgc
     5641 ttgttttgag cagcatagat ggcgttaaac ataacaccca aatttcctga ggttggattt
     5701 ttcatcccta ctggtgcatc aatcccagaa gccacaaagc ggtgctcttg gtcttccaca
     5761 gaacgagctc caacggcatg gtagctgacc aagtcatcca ccaagatcag atttgacgga
     5821 taaagcatct catctgccgt tgtcaaacca gtctctgtaa tcacgcggta gtgcaactgg
     5881 cgcacagcct gcaagccgtt aatcaggctt ggagccttag aagtatctgg ctggtgaacc
     5941 aatcctttat agccgtctcc attggtacga ggcttagcag tatacacgcg catgaccatg
     6001 aaaatcttgt ccgctacctt cttttgcaag gcggataaac ggcgagcata ttccaagacc
     6061 gcctcttcat tatcagaaga gcaaggacca atcaccaaga gaatacggtc atcttcccct
     6121 gaaataatat ctgccaattc cctgtcacgg ctttccttga gtctcaaggc ttctgcagac
     6181 aattgggttt cagccttgat gacttccata tcgatttctt gacctttttc aataaatgcc
     6241 atcttattct cctaacgttt tgtaaatttc tctgacaaga gcttctgtgt tatcccaacc
     6301 caggcaaggg tctgtgatag acttaccaaa tacttctggc tcattttgtc gaccatcttc
     6361 cagataagac tcaatcataa aaccacgaac gaactgctta attttttcat tccaagcacg
     6421 gttaatcaag gtctggcgga caattcggat ctgttcaata tactgcttac cagaattgtc
     6481 atgattggta tcaatgatga taaaaggatt ttccaagccc attttctcat actgggcaat
     6541 ggtatcaatt aaattgtcat aatagtagtt gggaatattt tttccatact cattaagagc
     6601 accacgaaga atagcgtgtg aaagcgggtt cccagttgtt tctacttctt ttcctaagaa
     6661 aaggaaactt tgtttgtttt gagcagcata aatcccatta aacatgacat tgagatttcc
     6721 agaggttgga tttttaaaac cagtagaaaa tcctgcccca cttgccacaa agcggtgttg
     6781 ctggtcttca actgaacggg caccaactgc catgtaagaa atcaaatcat ctacaagcgg
     6841 aaggttttca ggataaagca tttcatcagc agttgtcatc cctgtttctg tgatgacacg
     6901 atagtgaaga tggcgaacgg ctttgattcc attgataaga ctaggcgctt ctgtcgcgtt
     6961 aggctggtga atcaagccct tatagccatc tccgttggta cggggtttgg cagtataaac
     7021 acgcataacc ataaagatac gatctgccac ttcttcttgt aggactgcca aacgcttagc
     7081 gtattcaagg acagcttctt cgttgtcaga tgagcatggc ccgattacca agagaattcg
     7141 ctggtcttct ccacgtataa tggcttctag ctcttgatct cgctgtgatt tcctctccaa
     7201 agcctgacct tctaattttg acaaggcacg aacttcttct atatttattt tagagctttt
     7261 tgctgtaaat accataactc atctccttat aaagcaaacg gatacccagt aaaaatttac
     7321 ttttcacaag atatccgcct ctaaactatc tcattttatt gtcttttacc gtgacagttt
     7381 ttaaatttct taccagaacc acatgggcaa agttcattgc gtccaatctg gctcaaatcc
     7441 aaatcttctg gcatacttgc ttggtgagca gcgatattgc gagtcgctgt tgtactgata
     7501 tgacgttctg cctgtggtct ttcttgttca tgaatttgtg ctttcatcat caagcgtgtc
     7561 acatcaaact caatcgaacc aatcatatca ttaaacatac ggaaaccttc tgcctgatac
     7621 tcaacaacag ggttgttctg agcatagcca cgaagtccaa ccgcgttacg caattgatca
     7681 agggcatcga tatgatctgt ccacttgtta tccaccactc gtagaatcaa aactttttgg
     7741 aattctttaa ctgcttcttc atcgcgtagt tttgaaacct gactatcgta aaccttcaag
     7801 gcacgttgga aaagctcttc cttgatggcc ttatcagaca agcctgacaa gtcttccatc
     7861 gtaatagaat cttcaggaag caagttgtac ttagcgaagt tcaaaattgc ctctagtttc
     7921 tcatcttgtt tagcacgcgc atgaccgtca acgacacgac caatcgtgcg tttgatcatt
     7981 gcctgaattt caggtgccaa gtcacggtct gcagtaatga catcataacg ttgagtatag
     8041 ataatctcac gttgttcacg catgacatcg tcgtattgaa ggacttgttt acgggtatcg
     8101 tagttatttc cttcgacacg tttctgagct gcttcaacct gacgcgtcaa catgcgagac
     8161 tcaatggcct cttcagacat gttcaagcgt tcaaagattc ccttcaagcg ttcagaacca
     8221 aaacgtttca tcaaatcatc ttcaagagat aggtagaatt gtgactcacc tggatctcct
     8281 tgacgacctg aacgtccacg aagctggtta tcgatacgac gactttcatg acgttctgta
     8341 ccaataacac aaagtcctcc aagttcacga acaccttcac caagcttgat gtcggtacca
     8401 cgacccgcca tgttggttgc gatggtaacg gcaccacgtt gaccagcatt catgatgatt
     8461 tgggcttctc tatagtggtt tttggcattc aagacttcgt gaggaacacc agctgcaacc
     8521 aatttcttag aaatgtagtc actagtttca accgctactg taccaaccaa gacaggttga
     8581 cccttttggt aacgagcctt aacgtcttcg acaaccgctt taaacttaga ttcgatactt
     8641 gcataaagaa ggtctgagtg gtcaatacgt tgaacaggac ggtttgttgg gattggaata
     8701 acacgaatgt tgtagatttc acgaaattct tcttcctcag tcttacctgt acccgtcata
     8761 ccagacaatt ttttgtacat acggaaaagg ttttggtacg tgattgaggc agatgtcttg
     8821 gtttcatcct ggattggcac accttctttg gcttcaatag cttggtgcaa tccatcagaa
     8881 taacgacgac cttccatggt acgacctgta aattggtcga caatcaagat ttcttgctct
     8941 tcgctcacca catagtcaat atcgagaagc atgatgtagt tggcacgaag ggcgttatcg
     9001 ataaagtggg tcaaagccac gttttcgatg tcatagaggt tttcaagttt gaagtagctt
     9061 tcagccctgt caatccctga atcagacaaa ccaatagtct tagactgcac atcgatgatg
     9121 tagtcatctt tgttcaaaga ttttacatag tggtctgcca tgtgatacaa ctgactggtt
     9181 tcaaccgcat tggcacctga tacaatcaaa ggtgtacgag cctcgtcaat caagatagag
     9241 tcaacctcat cgaccaaggc atagttaagc ggacgttgta ccatgttttc agcgcgaacg
     9301 accatgttgt cacgaaggta gtcaaatccg atttctgagt tagttgagta agtaatatca
     9361 cactcatagg cttctttttt ctccattgga gatttggtag ccaagttaat ccctactgac
     9421 aaaccaagcc aagagtacaa ttcacccatc tcagtcgcgt cacgttctga caggtattca
     9481 ttaaccgtaa ctacgtgaac ccctttacct gaaagggcat tgaggtatac cggcatggtc
     9541 gcagtcaagg ttttcccttc ccctgtacgc atctctggca cgtcaccatg gtgaagaaca
     9601 atccccccca tgacctgaac cttataaggg aagagaccta ggacacgttt ggcaccttca
     9661 cggacaaccg caaatgcttc gtaaagcaat gaatccagtg attctccatt ttgataacgt
     9721 tccttaaatt caactgtttt tgcttttagt tggtcgtcag tcaaagcagc catttggtct
     9781 tcgtatttga aaaccttgtc agccatcttt tccagacgac ggatttctcc tttatcattt
     9841 tcgataattg tttttaaaat attagccatg tttttcctta ctttaaattc cgaatatttt
     9901 agaatgttcc tttaatttta gcacaattac tgattatttt caaggaagaa tccccatttt
     9961 gaaaaggaaa aagaggttag ttttcaagct aacctctccg acttttatga ctgtttaatt
    10021 gccaaaaatt ggcaaaatgg caaatgtgat aaataagtta atccaaagag aaagacagca
    10081 aatcagtcca aaaacaaaga gactgacttt cttggacagc aaggccatca aaatggacag
    10141 aattccaaaa actagcaaga ctttctgtat gatgaagatg tcaatcctta cccccagaat
    10201 cgctctatcc caagggataa agaagaaaga cagccagatc agcagaacta aaccaatata
    10261 aaccttggaa tagcgtgtaa taaatgattt tagatgtgcc ataagccatc tcctaggatt
    10321 gtatatcctt tacatattgt atatgtccat tttcgacaac taatacatct gtacaaaggt
    10381 ctgaaatatc ttccgatatg tgcgacgtca ggataaccag accattttct tttttcaggt
    10441 aagataaaat gacctgtttg gttaacctca cacttttctc atccaaagca ttcataggtt
    10501 catcgagaaa gagtatagaa ggagaggaaa taaaggcttg aatcaaggcc attttttgct
    10561 ttgttcccaa ggataaatga cggtattcaa cgtcttcaaa ttcctgtaaa tcatagtatt
    10621 gaatccaata ggcaattctt ttttccgtaa ctttagatga aaaatacctt aacagttcca
    10681 aattttctct cagggataaa tgagataaaa actcgacttt ttcaattaaa attcctgcat
    10741 cctgaatata atgatttttt accccgtaaa ctttaccatc ttgaagaact tttcctttgt
    10801 caagcttaat ataaccagca agtatcttta aaagaaccgt cttgccagat ccattatcac
    10861 ctttaagtcc ataaattttt cctgattgaa acaccaaatt caactgattt aaaataggtc
    10921 ttttcccata aattttctgt ccatttataa tttcaagtct catcgtttat cctttcttaa
    10981 atttctcttc caagcgacta agaatcaaca taactaataa gcttaagagt actaaataca
    11041 ctgttacaat atgatccatc atccattctt gtcccacaaa tgccaaaaaa tagagaaaaa
    11101 aagaaaaacc agcgcccgca ctaatcagtg ctatcagata agacagtagt aagtacaaca
    11161 tcattaactg agcaagtaaa aagaaaagtg cgactgtttt gaaatgaatt cctagcggaa
    11221 gtaaaccaag aaaccagaca aacaaatctt gagtaaaaaa tttagaaatg acaaccaatc
    11281 gtttccttgt ataatcgaaa aaggagcttg attgatagcg aatcaagtga tggtaatggt
    11341 ctgccaaatc actttcaagt aaaatgacag agagccatcc taccatgaaa agcgaaaggt
    11401 ttaacaagag tttggggatt tctaaattcg cttcagaata gtcattatag gaaaaaattg
    11461 aaatcaattc tctcttgaaa aagataacag catcctgctt ttgaaccgct gttgacaata
    11521 gatagtttat caaaatcatc atcagaacta tacgaagtag taaaacgatc tttctcttta
    11581 gccaaatatt gttaaaattc ttaaacatta tcttcatatt ttttaagtac catataaggg
    11641 acaacataaa ggatataagg agtaaagagt ttcatcaaac ttacatcccc atagctagct
    11701 tgcatcaaac tcgtcatagg aaccatatag taaggcaagg cactatagag cagcatagaa
    11761 ccaagccaaa gaaacatcaa ataggtgatt gccgaacgag tcacattccc cacataatca
    11821 actatttgta aaaaatagat tgcattgatg aaaataccga ttaataggac acaagtaaag
    11881 aacaaatagc tttttatctc tccatctagg agtctttgta aaccactccc atcagaaaat
    11941 agagaattcc attcaagagg agaaaaagtc ccaaagaaat aggttataat tgcaattatc
    12001 agcacagtca ctaaatatat caaacaaggg atacttgcaa cttgcaaagt caactttctt
    12061 tttaaccctt gatagctcac ttctcttcca atactcaatc gtaaaacttt atttttcagc
    12121 tcaatatatt gaaaacctag caaaacaatg atgattggaa taataaatga ttgataaaaa
    12181 cctagattga actggagaat acgaaaatct aatgctgatt ttaataagtc atttttaatt
    12241 gcttgtccat cgaatttagc tagatcgggc gcatgaatat caaaaaagaa ataagtcatc
    12301 aaaacatcac gataaaccaa agcaagaaaa agcaaagtaa gcaagacata aacaaattta
    12361 tgccttgcaa aagttaagag aggaatcttc ataaattata cctactttac gaattaattt
    12421 ggtgaccaac tacctgaaat ggcagctgat aaatcccacc aattttgtct tgccatatct
    12481 actgcataaa catagccaac cgttgcccaa ttaccatgag ttgaacgagt accacaggtt
    12541 gttaacgatt ctccactacg agcttctcca ttagaattta ctatacgatg cttaacagtc
    12601 atgggatgta aatttctaca aggatttaag ttcatgatat attccgcacc ttcattttct
    12661 ttagtcaaat aacctgttct cttataactg gtagtaataa caaaattact aaaagcacct
    12721 atctgatttg cttctacgaa agtagttcca acagcactta tcattaaaac aggaataacc
    12781 attttcaata atttcttaat tttcattttt tctgtttcct ttcatttttt attcaagaag
    12841 attccttata acttctattt taaatataga cttattctaa acgatggtat atcatacaaa
    12901 ttcctccttt acttattatg tatagactct aatttcttta tccccaaaac catgtttcaa
    12961 accagataag ttgaatccaa ctccaaatca aaaatccaga gtttttcggt aaattcttca
    13021 aatatttttt aaagcccatg actttttccc tcctttttga tataataata ccaccttttt
    13081 ctacgttttt tataaatgta gaaaatattc acatttacct aggaggaaca atatgagatg
    13141 ggattatgga caaattttta aagaaattcg aaagtcaaaa ggattgaccc aacaagatgt
    13201 atgtggacaa gtcatacatc gaacaactct aacaaatatt gaacacggta aagttattcc
    13261 cagttttgaa aacatggtat ttcttcttga acaaattgat atgagcttgg cagaattcaa
    13321 gtatatatgc aacgaatacc atcctagtaa aaggcgagat attattgtag agagccaaaa
    13381 tccgtctact tttcaagata ctagaaaaat ggttgaactc actgagaaat gtcaaaaata
    13441 tcttaagaca catcacgatg ttcctattca aaatatctat cgtcatacaa aaattgtcac
    13501 agagttacga actaaaggat tcaaaaacaa ccacgtcttg aaagatttgt atgaagaaat
    13561 ttgggactat cttgaaccta tggatacatg gtacattagt gatttgaaat tgcttggaac
    13621 cattctcttt ttctttcctt ctgaaaatct tccccttctt attgatagaa ttatgaaaac
    13681 catcgagaaa tataaatact tccgagaaac aaaagcattt ttatcatctt ttttagccaa
    13741 tctctccact gtatattttc aacatcattt attcaaagaa tgtgaaacaa tcaccctaca
    13801 acttttggta ttagcagaag aattaaaaat atacgatatt cttggattct ctcaagtacg
    13861 cttgggaata ctacaacata actccgattt gattgataaa ggaataactt tattacgact
    13921 aaccaaagaa gaggcattag ttaaaatact cgaaaaagaa attaatgatt tttcaaacct
    13981 ataatttgta ataactctca tcattttatt atagtagatt gtaatagaat gtaatagaat
    14041 gtgaacgaat caattagggg atctaaagca ttttatacca atgttttata agttgtattg
    14101 tattattcca tattcaatcc actatacaag atgaatattt ctctcaagtt tcataatcaa
    14161 aataaaaatg actgtatcta tagctccata aaaaaatcat agatacagtc attttttagt
    14221 tcctacacct tcctttatgc acacctttag acttccctag ttcccgtctc aagtcaagca
    14281 tttttttgaa acagatatag tctaaccagt cactagcaaa agcgataagt agtaataata
    14341 gccaaacacc ccaagacttg atgtctgcca catttctcaa gacgatattg aacaacagga
    14401 ccgaaaaaac tgtccaagcc agactaaaaa gagcatagag ggtgatgtaa atccagtaaa
    14461 aatagtaaaa aattgagaaa atcttactat ctctgtttgc cctttcaatc cagctgaaaa
    14521 agtaaaaaca gggtaataag attaccattt taaacaagtg ttccatcaca aacctcctct
    14581 tttaaattgt tttctttagt ttcgttctat taaatgttat taccaatata aaccttggaa
    14641 tagcgtgtaa taaatgattt tagaggtacc ataagccacc tcctacaaat agaaaccgat
    14701 ataaatcaat gccttccacc cttagacttc cctagttcct gtctcaagcg aaacatttct
    14761 ttgaaacagg aataagttaa ccaattcata ccaatagcta gcagaataaa aagaaaccaa
    14821 atgccccata acttgatatc tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa
    14881 acaactgtcc aagcaaggct aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa
    14941 taaaaaattg gaaaaaactt actatttctg ttggccttct caatccagtt atcaaaataa
    15001 aagtacggtg ctaaaagtaa gaatttaaac aaatgttcca tcaccgacat caccctcctt
    15061 cttttgatag cgttttctat tattttatta tatcaaaaaa atccggaact gtcattccag
    15121 attctacttt tttatttgcg ttttcttgcg atgagatgaa tcggtgttcc ttcaaaaaca
    15181 aaggccttgc ggatttgatt ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct
    15241 tcattgacaa agatgacaaa ggttggtggt ttggttgcca cttgggtcgc atagaaaatc
    15301 ttgagacgtt ttcctttgtc tgtcggtgtt gggttgatgg caatggcatc catgatgaca
    15361 tcgttcaaga cagctgatgg aatacgtgta ttttgacttt cgctgatttg cttaatcatc
    15421 tcaggaagtt tgtggagacg ttgcttggtt aaagctgata caaagataat cggtgcgtaa
    15481 ggcaggtatt ggaactgctc acggatatct tcttcccagt ttttcatagt gtggttatct
    15541 ttttcaagcg tatcccactt gttgaccacg ataatcatcc ctttaccagc ttcatgggca
    15601 aatcctgcga tacgcttgtc gtactcacga atgccttctt ccgcattgat gaccatcaag
    15661 accacatctg aacggtcaat agcacgcatg gcacgcataa cagagtattt ctcagtattt
    15721 tcataaacct taccagactt acgcatacca gccgtatcaa tcatggtaaa ctcttgacca
    15781 tctgtatctg taaagtgggt atcaatggca tcacgagttg ttccagcaac aggactagca
    15841 ataacacggt cttcgcccaa gatagcattg atcaagcttg attttccaac gtttggacga
    15901 ccaatcaggc taaacttaat gacatctgga ttttcttctt catattcatt tggaaggttt
    15961 tccacaatcg catctagtac atcccctgta ccgataccat ggacagatga gataggcagt
    16021 ggttcaccca aaccgagggc atagaaatca tagatatcat ttcgcatctc agggttgtct
    16081 accttgttaa ctgcgaggat aactggtttg tgggtcttat aaagcttacg ggctacgtat
    16141 tcgtctgcat cggtaattcc ttccttaccg gacacgacaa agacgataac atctgcttct
    16201 tccatggcaa tttctgcctg gtgcttgatt tgttccatga aaggagcatc aacatcatca
    16261 attcctcctg tatcaatcat gctaaaagaa cgattgagcc actcacccgt tgcataaata
    16321 cggtcacgtg tcactccttc gacatcttct acaatggaga ttcgctcacc agcgatccga
    16381 ttaaataggg ttgatttccc aacattggga cgtcctacaa tggcaatagt tggtagggcc
    16441 ataatttctc actttctaca ataatttctt ctgttcaaga ttttttctag ttgagcttgg
    16501 ttcagcttga ccaaactgtt ctgctagacg ctgactccag cttgtggtcg cacgcgcccc
    16561 agcatagtca gcctgaacac ggtcataagc ttggattgcc tcagttgact gttcttggta
    16621 ttcttcctca aagacaacat tctctagtgg cagtctcggt ttcatatcat gatgttgatt
    16681 tggcacaccc agtgccatcc caaagacaga ataggtgtag tcaggtaggt taaagagctc
    16741 tgccacttct tcagacttgt atcgaaccaa accgataatc acaccaccat agcccaagct
    16801 ttcagctgcc aacaaggcat tttgtccagc aagagctgca tcgaccgaac taatcaagag
    16861 accttccaca ccttggggtt ggaaggtgtc ggtatgaagt cgggctccct tttctgctcg
    16921 gttcaaatct ccgacaaaga gaaggaaaac agcagactgg cgaatggctt cttgaggtac
    16981 caattcatac aaggcatctt tcttctcttg acttcgtacc acaatcacag agtaggattg
    17041 gaaattcttc caagatgatg ccatctgggc tgctgtcaaa atctcattta agtctacttg
    17101 gggaatttct tgctctttaa acctgcgcac tgaagtatga gccttcatca atttaatggt
    17161 ttctgtcatc gacggtttac tccttctaaa cgagtctcct cagccaaata acggatgcgt
    17221 tccatgaccc gtctggcttc ccaggtttcg tcatttccat gtttcacttt cgcaaaatgc
    17281 ttctccaaat cttcaaagtt gaagttggat gtgaaaaagg tcggtaaatt ttcctgcatc
    17341 cgatattgga gaatgacctg caggatttcg tcacgcaccc aaacggttga ttgctcggcg
    17401 ccaatatcat ctaaaatcag gacctcagac agcttaatct catccaccaa ggtcttaaca
    17461 ttgccatcac tgatagcatt tttgacatca atgacaaagc taggatagtg gaggagagtt
    17521 gatgaaacac cacgtttttc tgataaatca tgagctaagg ccgccaccat gaaactttta
    17581 cccacaccaa agtctccata taagtaaaga ccttttcgaa tagctggata ttgctccacg
    17641 aaggctagta gcttttcaaa aactggtaag cgccccaaat catccaagtc aacttgagcc
    17701 aaactagctt tcttgagact ggctggtaga ttgattaact tgagacggtt cttaatagcc
    17761 gcttcttttt cagccgcgat tagctcagga gtttcttcat atgaaacatc tgcatagcca
    17821 tgattcttaa ccaaaatcgg cttgtagcct ttggcaatat aatccgtatc cccacggaga
    17881 aacttgtcac gctcggtgat gtactgatta aacttggaga tactgcgatt taattcctct
    17941 ggagtcaagg attcttgctg gataaaagcc gcaacatcag ggtccttcat gattttctgg
    18001 accaaatctt gataataaaa acggctgggt tgacgtttga gtacgtctcc gacactttcc
    18061 atctaatctc ctcctttttc taatcgagct aatagttctt gcttcttacg ttctagttcc
    18121 agacgagttt cctcgctggt ttcattctta tattcaggat tactccattt aggaacattg
    18181 gtttttactg gggcagtctg attctgtttt tgtgtttttg ctttctgtcc tcgctcacgg
    18241 atgcgcaaga ctgccttttc tgccgaatga atcttttgat aggcatagtc attggctacc
    18301 ttcatggcat atttctcatt gatatttgcc gaatcgacct tattaaaggt caataaaaga
    18361 atgatattga tgacttcgtc cagcaagccc aagccagcca tctgttgcaa gagttctctt
    18421 tctgtttggg taatggttcc cttgcgtgtt tgcttgattt ctgccaagaa ctgcagggca
    18481 gttttacttt tggcttcttt gataatggtt gtttccttaa gactaaagtc agaggaaact
    18541 ggtttttgag caattttttc acgcatgcgt ttggttgaaa taacctgaga aacagctgtt
    18601 gacttggcca attgataggt ttcaaaccaa gtccatttct tctcctcggc aatagcaaag
    18661 aggtttaaga catcggactg ctcatccgca aaacgaagtc catctcgagc catcagctgg
    18721 cgaaaatgtt ccaagtcaaa atcattggcc actttcttct tgagaccaag gtcttcttga
    18781 ctgcctagtt ctgccaattc tggaaagact tgattgagtg agacaggtat ttcttcacca
    18841 tcagcacttt caactttcaa atcctccaca gcagagtcgc caatcttttt ctctaagagt
    18901 ctgcgataaa caggatactc caagaagtct tgactagata gaggagcatg gagggctagc
    18961 tgataaacat cccccttttg atagagggtc aagagattaa aagcagataa gattttcaat
    19021 gattttatca gtctatccat cccaaagttg agatgattga ggatacttga aaaaagatat
    19081 tcctttctac cattatccca aaaactgatt gtataaagat aaaggctcag tgcctcctga
    19141 ccgataatcg ggaggtagca ctgtaccaga gatgaggtat cttgcgacac ccgattattc
    19201 tttagataag aaaaacggtc aattggcttc atttatcttt cctttttctt tttagaggac
    19261 tgggtgattt gttggagcaa gctctctaac tcactgacat ccttaaaact acgatagaca
    19321 ctagcaaaac gtacataggt aatctcgtcc aattcagcca actcctccat gacgagtgaa
    19381 ccaatgtcct cactttgaat ttcattttca tttcgaccac ggagtttctg ttcgatacga
    19441 ttgactacca tgttgatttc atcacttgac acaggacgtt tctgggctga gcggataatc
    19501 ccattaaaga ttttatctct ggagaattgt tcccgtgtgc catctttttt aacaaccact
    19561 aaggttcttt cttctactcg ttcgtaggtt gtaaaacggt gttggcactc gtcgcactct
    19621 cgtcttctac gaatggtgtt cccttcttct gcttggcgac tatcgataac acttgacttg
    19681 gtagccccac attttggaca acgcatcctt tccctcctta tcgttttctt ttcattatac
    19741 cattttttaa gcgattccca aaacaattct tctttttgct tgacaagttt tttgttttgt
    19801 tgtattattt aattaagaca aaagggtaag agaaaggaga ctaagatgtc ctggacattt
    19861 gacaacaaaa aacccatcta tttacagatt atggagaaaa tcaagcttca gattgtttcc
    19921 catacactgg aacccaatca acaacttcca accgtgaggg agctagctag tgaggctggt
    19981 gtcaatccca ataccatcca aagagcctta tcagaccttg aacgagaagg atttgtctac
    20041 agcaagcgaa caactggacg atttgtgact aaggataagg agctaatcgc tcagtcacgc
    20101 aaacaattat cagaagaaga attggaacac ttcgtttcct ccatgaccca ttttggctat
    20161 gaaaaagaag aactaccagg cgtagtcagt gattatatta aaggagttta agcctatgtc
    20221 attactagca tttgaaaatg tatccaaatc atatggagca acaccagccc ttgaaaatgt
    20281 ttctcttgac attccagctg gaaaaattgt cggccttctt gggccaaacg gctcaggaaa
    20341 aacaaccctg attaaactaa ttaatggcct cttacaacca gatcaaggac gtgtcctcat
    20401 caacgacatg gacccaagcc cagcaaccaa ggccgttgta gcttatttgc ctgatacgac
    20461 ctatctcaat gagcaaatga aggtcaaaga agccctaacc tacttcaaga ccttctataa
    20521 agatttcaat cttgaacgcg cccatcatct acttgcagac ctgggcattg atgaaaatag
    20581 tcgtctcaag aaactatcaa aaggaaacaa agaaaaggtt caactgattt tggttatgag
    20641 ccgtgatgct cgtctctatg ttttggatga acccattggt ggggtggatc cagcagcccg
    20701 tgcttatatc ctcaatacca ttatcaacaa ctactcacca acttctaccg ttttgatttc
    20761 tacccacttg atttctgata tcgagccaat cttggatgaa attgtcttcc taaaagacgg
    20821 aaaagtcgtc cgtcaaggaa atgtagatga tattcgctac gagtcaggtg aatccattga
    20881 ccaactcttc cgtcaggaat ttaaggccta agcaaaggag attatttatg ttttggaatt
    20941 tagttcacta cgaatttaaa aatgttaaca agtggtattt agccctctac gcagccgtgc
    21001 tagtcctttc tgccctcatc ggaatacaga cacaaggctt taaaaatcta ccttaccaag
    21061 aaagtcaggc tactatgcta ctttttctag ctacagtctt tggtggcttg atgcttacac
    21121 ttgcgatttc aaccattttc ttgattatta aacgcttcaa aggtagtgtc tacgaccgac
    21181 aaggctatct gactttgacc ttgccagttt ctgaacacca tatcatcaca gccaaactaa
    21241 tcggtgcctt tatctggtca ttgattagca ccgctgtatt ggctctaagt gctgttatta
    21301 ttctggcttt aacagctcca gaatggattc ctctttctta tgtgattaca tttgtagaaa
    21361 cacatctccc tcagatcttt cttacaggta tatccttcct actaaatacc atttcaggaa
    21421 tcctctgcat ctacctggct atttccattg gacagctttt caatgaatac cgtacagcac
    21481 tcgctgttgc agcctacatt ggtatccaaa tcgtcattgg atttattgaa cttttcttca
    21541 atcttagttc caatttctat gtcaattcac tggtaggact caatgaccat ttctatatgg
    21601 gagcaggtat agccattgtt aaagaactca tattcatagc tatcttttat ctcggaacct
    21661 actacatctt gagaaataag gttaatttgc tttaaataat ttttacctag atatgtaaca
    21721 tactcataga acaaaagaga ccaggcaaaa agtctttaaa attagaaaac gcatagtatc
    21781 aagtgttaaa aatccttgat attatgcgtt ttttgtgagc tgacttattt cctttcgcta
    21841 tatcgcaaaa tgaaataaga acggaacgat gggattttgg aattcaaatc aatttataag
    21901 aatgttttag aagtaatatt atcctattcc agattcagtt cactatacaa ttgagttttc
    21961 aagcaacctg tttacataat gtgtacataa ttaggttcgt gattccaccc ttttcacctt
    22021 taaaaacctc gctttcgcaa ggctcttcta tttataagat aaggcacgtt taaaggtttt
    22081 ccaaatccct aaatcatccg tttgaagaac gagactagca tacatgcgtc cgataaatcc
    22141 tgttgctacc accgcaaaaa tcactgtaat agcaagtgaa atccatgctt ctgctccccc
    22201 cgcatagcca ttaatcgttc gaaacggcat aaagaaggtc gaaataaagg gaatataaga
    22261 accaatcttc aagaggagat tgtcaccagc tgcacctaga gctgtcactc caaaaaaacc
    22321 acccataatc aaaatcatca aaggcgacaa ggctttccct gagtcctcag gacgagaaac
    22381 catagatcct aggaaggctg ccaagactac gtacatgaaa agactgatca aaataaagag
    22441 caaggtattc agtgagatag catctcccaa gtgatccaaa ataccagact gagccaagaa
    22501 tggcaaatct ttaaagagca aaacggcagc cagaccacct acaacataga tcccaatatg
    22561 cgttaaaatc actagaaaca gagccatcat ccgcgcatag aaatagtgac ttgcccttat
    22621 gctagaaaaa acgacttcca taattttggt gcctttttca ctggcaactt cctgagctgt
    22681 tacacccgca taggtaatca gaatcatata aagaaagaat cctaaggcac ctgctgcaat
    22741 tgtttgaata aactttttat tttccttggc ttcatcaatc ttttctgtga attgaattgt
    22801 ctgcgctaag cgtttttcct gctcttgaga caaggaagca gttgaacgat taagctgatt
    22861 ttgcagttca ttgagtgtac ctgtaacctc aaatttaatt ccattttcaa gcgatgtttc
    22921 gccatgataa actgccttta gaacactatc ttcttgatca atggtcaaat aaccttttaa
    22981 tttttcttct ttaattgctt ctttggcact tgcttcgtct ttatagtcga agttaacacc
    23041 atttacattc ttcagtcctt ctgctacaga tggcactgtt gtcactactg ccactttatt
    23101 atttttagcc atagaagaac cttggatatg cccaattcct acagagattc ctaaaaagag
    23161 gaacggcgaa atcaccataa agaagaaact ccatgactcg acatgtcgaa gataggtttc
    23221 cttgattaca acccacatat ttctcatact tccactcctg attctagttt aaagatttca
    23281 tcgatagttg gcgcttgttg gtcaaatgtt gcgatatatt gaccttgagt caagattggg
    23341 aagagttccc ttccagcgct ctcatcctcc aaaatcaatt tccaactgcc ttgtttggtc
    23401 aagctcacct gtttgacatg aggaagattt tccaattctt ccttgcttcg ttcacttgaa
    23461 acaaagagac gcgttttccc gtattgattg cggacatcct gaactggtcc gtgcaagacc
    23521 acacggccat ctcggatcat cagaatatcg tcacaaagtt cctcaacatt ggtcatgaca
    23581 tggtcagaaa agataatggt tgctccgcgc tctttttcct gaaaaatgac ttgtttgagc
    23641 aattctgtat taactgggtc caatccacta aaaggctcat ccaagataat caggtctggt
    23701 tcatgaatca gagtaataat gagctgaatc ttctgctgat ttccttttga cagactcttg
    23761 attttatctg tcagctttcc tttcacttcc aacctcttca tccattgagg gagtttttct
    23821 ttgacttctt tggcatccat gccttttaga gtcgccaagt agcgaacttg ttcaagaact
    23881 gtcaatttag gcatgagact gcgttcttca ggcagataac caatccgagc ataggtctcc
    23941 tgacgaatat cctgaccatc cagaccgatt tctccctgat attctaggaa tttcaaaata
    24001 ctatggaaaa tcgttgtttt tccagcacca ttttttccga ctagtcccaa aatacgacct
    24061 ggtcgcgctt gaaagtcaat accaaacaaa acttgcttgg agccaaaact tttctctaga
    24121 cttcttactt ctagcatctt tcacctccga aatttcttgc actcattata ctcctttttg
    24181 atagccttta caatgttttt gtccattttt agaagactat tgctgtgtaa aatatggcct
    24241 ggagcacttt tatactcaat gaaaagcaaa gagcgaacta ggaagctagc cgtaggctgc
    24301 tcaaagtaca gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt
    24361 gaggttgtgg atagaactga cgaagtcagt aactatatct acggcaaggc gaagctaacg
    24421 tggtttgaag agattttcga agagtattag tgataaatcc attatacagt agcaaactca
    24481 atttatacct tccgctcctc aactgtctat ttttaatcct gaattgttat ttgagtaact
    24541 cctttttcct cataaagttt tcttcctcta aaacttctgg aaaaaagcga atagtttcag
    24601 acaacatttt tataagaaac aagttcatct gtcatttcaa gaaggagtaa tcctttatct
    24661 actaatggac ggaacggaat tcaaccgctt gtccgatata ttttctaagg attatatagt
    24721 aaaatgaaat aaaaacagga caaatagatc gggacagtca aatcgatttc taacaatgtt
    24781 ttagaagtag atgtatacta ttctagtttc aatctgctat atctattatg cacaccccta
    24841 taggatctaa tgaaaatcac aacaggctca ttcatagatg gttacctcag gaaactaaga
    24901 aaacgactac aaaggaagtc gcattcatcg aaaagtggat taacaactat cctaaaaaat
    24961 gcttgaacta caagtccccc agagaagact tctggatgac taacttgaac ttgaaattta
    25021 gcaataatta attcaccatc taactatatt tagtaattat ttcagaactg attaatatta
    25081 aaattaacta acaattcaaa ggattcatac tagccataaa ttacgtccat cagagagaga
    25141 ctcttactac ttttatattt tagtctttct ggcttcagga tacatataaa ctttagggaa
    25201 aatgactatt cgaaagcgcg aatgcctcaa aattatctca gataagctat tcgaaactta
    25261 gaatgctttt aaatttatgg aattgcgatt attcgaaacc tagaatgcat ataaccttta
    25321 gttgacagac ctattctaag tctcgaaggg ctatttactt tctattcctt atcaaaaaag
    25381 actcattccc cctttctcct ccaaaatatg gtatagtaga aatatactat ctatgaggag
    25441 tttacatgtc acaggataaa caaatgaaag ctgtttctcc ccttctgcag cgagttatca
    25501 atatctcatc gattgtcggt ggggttggga gtttgatttt ctgtatttgg gcttatcagg
    25561 ctgggatttt acaatccaag gaaaccctct ctgcctttat ccagcaggca ggcatctggg
    25621 gtccacctct ctttatcttt ttacagattt tacagactgt cgtccctatc attccagggg
    25681 ccttgacctc ggtggctggg gtctttatct acgggcacat catcgggact atctacaact
    25741 atatcggcat cgtgattggc tgtgccatta tcttttatct ggtgcgccta tacggagctg
    25801 cctttgtcca gtctgtcgtc agcaagcgca cctacgacaa gtacatcggc tggctagata
    25861 agggcaatcg ttttgaccgc ttctttattt ttatgatgat ttggcccatt agcccagctg
    25921 actttctctg tatgctggct gccctgacca agatgagttt caagcgctac atgactatca
    25981 tcattctgac caaacccttt accctcgtgg tttataccta cggtctgacc tatattattg
    26041 actttttctg gcaaatgctt tgacacgtaa aaaatccgtt tggtttccca agtggatttt
    26101 taaagcgtag attaactata gcttgatact aaatatactt tggtatggaa atcatgcata
    26161 tttttcgata gtgaggcgag gacttaccta gccttcccgc cgtgatagaa acacctgaaa
    26221 tctaatggtt tcaggtattc ggaaactttg agcctagtgt ctcaaagttt aggtatggaa
    26281 tttcgaagaa agtcgctacc gtccgtaatc acttaaggaa aggctcaaaa atattgtttt
    26341 caaccacaaa atccgtttgg tttcccaagc ggattttgtg ctttattttg aaacttcttt
    26401 tgcaagaaca aagttcccaa gtgtggcaga accatttcct gcgactgctg gcgtcacgat
    26461 atagtcacgc acatctggta ctggtaggta accattaaga agagatgtaa atttctcacg
    26521 gacacggtcc agcatatgtt gttgagccat gacccctcca ccaaagacaa tcacgtctgg
    26581 gcggaaagtc actgtcgcat taaccgcagc ttgagcgata tagtaggctt gaacatccca
    26641 aacagggttg ttgagttcaa tagtttcccc acgtacacct gtacgagctt ccaaacttgg
    26701 accagctgca taaccttcta gacatccctt atggaaagga caaacaccct taaactcttt
    26761 ttcaatatcc attgggtgtc tagcaacata ataatgaccc atttcagggt gacccacacc
    26821 accgataaac tcaccacgtt ggatgacgcc tgcaccgata cctgtaccga ttgtgtagta
    26881 aaccaagttt tcgatacgac caccagcatt gttacgggca accatttcac cgtaagcaga
    26941 gctgtttacg tctgttgtga agtacattgg cacgtttagg gcgcgacgaa gggcaccaag
    27001 caagtccaca tttgcccagt ttggttttgg agtcgtcgtg ataaagccat aagtttttga
    27061 gtttttgtca atatcaatcg gcccaaatga accaactgca agaccagcaa ggttatcgaa
    27121 ttttgagaag aactcaatgg ttttatcgat tgtttcgatt ggagttgttg ttggaaattg
    27181 tgttttttct acaacgttaa agttttcatc accgacagca cagacaaact ttgtaccgcc
    27241 cgcttccaag cttccatata attttgtcat gataaacctc ttgtttttat tttctttatt
    27301 atagcatact tcgaaagtct aaatgtctct attttttaga ttttcctctg taaatcttac
    27361 tatctaataa aaacgaacaa acatgtcatt tgttcgtttt cacattagag aggattgatt
    27421 agattttcac ttcgatcaca gcatccccct tcgcaactga acctgttgcg actggagcta
    27481 ctgaagcgta gtcagctgta tttgtaacga taaccattgt tgtatcatca agtccagctg
    27541 cagcgatttt gtttgagtca aatgttccaa gaacatcgcc aactttcacc ttatcacctt
    27601 gagcaacttt tgcttcaaaa ccgtcaccgt tcatagatac agtgtcaata ccaacgtgaa
    27661 tcaaaacttc agcaccattt cttgttttca aaccaaaagc gtgccctgtt ggaaaggcaa
    27721 ttgaaacttc agcatcagct ggtgcataga ccacgccttg gcttggtttc acagcgatac
    27781 cttgtcccat agctccactt gagaagactg ggtcattgac atcagcaaga gcgacaacat
    27841 caccaacgat aggagttaca agtgtttcat tttgaagagt atcagtctct tccactactg
    27901 gtgcttctac tgtaggttct gtaactgttt ctgcggttgc tgtggcagct tcatcagcaa
    27961 agacactagc tgcttttgtt ttcccgtaga aataagtgaa agcaaaggcg gcaacaaagg
    28021 acataatttc cgcaattata tacataggga ttgaaccagc cttaatagaa aggaatccaa
    28081 taaatccagc cgctcccaat gaaactgcaa taacgtgagt taaacctgct acaaaggcac
    28141 caattgctga accagcaagg gcgcagaaga atgggaattt gtatttcaag tttaccccaa
    28201 agagagcagg ttctgtaata ccaaggaagg cagaaacagc agccgaactg gacaatcctt
    28261 ttgttttagc attttttgtc aagaagtaga ctgcaagagt agccgcacct tgtgctacgt
    28321 ttgccattga agcaacaaca aagatgtagt ctccaaatcc agtaccgtta ttattgtatg
    28381 cagacaacaa ttgtgtttcg attgctggga atgattggtg aagacccgtc ataacgatta
    28441 atgagtaagt tccaccaaat agccccatac caaaggcacc tgttgtgttg tacaaccata
    28501 caatcgcatt tgtaatcgca tcagacacaa ccaacataac aggaccgata actgtaaagg
    28561 taaggaatcc tgtaatcatc actgataaaa gcggtgtaaa agtgaagtca actgcagatg
    28621 gcaatttctt gtggaagaat ttttctaaat tagctaacaa ccagactgca acaaggactg
    28681 gaataacctg ataagcatag tttgtttgtg taacatggaa acctaagatg tcccagaagg
    28741 cttctttacc acccaaatct ggagtggtca taatcatacc gattgccgca ccaaggaatt
    28801 ggtttgctcc aaagcgcttg gctgctgaaa taccaaccaa aacagggagg aacataaatg
    28861 gagcagcaga catcaattga atcatagctg agaagccttt gatagcagga tacatatctt
    28921 caattgattg aggtccaaac aaatctggtg atgtcaagaa gttccgtaaa gccatcaaga
    28981 gaccacctgc aactaaggca gggataatag gaacaaaaat gtctgacaaa agtttaatca
    29041 agtccatcaa tggattgaac tttttgtctt tattagcaat ctctttcaaa tcatctgtcg
    29101 aaacttcttt caaaccagtt ttcttaataa tttccgcata tacgaagtta acatcaccag
    29161 gaccaatgat aatttgatat tgtccgttag tgctaaaagt ccccttgaca tccgcattat
    29221 tatccagagc tgcttgattc acttttgctt cgtctttcaa taccaagcga agacgtgtcg
    29281 cacagtgagc tgcagctaca agattatcct taccgacagc cccaatgacc tcggcagcaa
    29341 ctttactata atccatagca aaaatctcct tatataacat tttgttcttg tttgaaagcg
    29401 attttattcg cccttacgac tattatttta tcatgtttta aaaatatgtc aagcgttttg
    29461 cagaaaaaat attctatcca cttagtgggc aagttttaga ttgattgttt ttgactgttt
    29521 catcaggaat tccttgaaaa ataagtttga aaccttggtt aaaaagtctt ttatttcatt
    29581 atctttcaca ttttcgtaaa aatttgattg atttcctgta tttattttat gataatcgtt
    29641 tgacagtttt tcctcttttt taacataaaa ggcttgcatt ttttatcgaa aacagttact
    29701 attaaaagtg ataagatttt tggaggaatg aatggaatgg acaactgagc gtcgttacag
    29761 actttatcaa gattggacgc aagaagaaat tcaacatata aaggaaaata tggcacaatc
    29821 tccatggcat actcattacc atgttgagcc aaaaacagga cttctcaacg acccaaatgg
    29881 cttttcttac tttgatggca agtggatcct cttttaccag aattttcctt ttggtgcagc
    29941 ccacggtttg aaatcttggg cacagctaga aagtgatgat ttggttcact ttaaagaaac
    30001 tggaatcaaa gttttaccag atactccatt agatagccac ggtgcctact ctggttctgc
    30061 catgcaattt ggcgataact tattcctatt ttatacagga aatgttcgcg atgaaaactg
    30121 gatccgtcac ccataccaga tcggtgcttt gatggacaag gagggtaaga ttacaaagat
    30181 tgacaagatc ttgattgacc agccagcaga ctctactgac cacttccgcg atccacaaat
    30241 ttttaacttt aaaggtcaat actatgctgt cgtcggtgga caggacttgg agaaaaaagg
    30301 cttcgtccgt ctctacaagg ctgtcaataa cgactacaca aactggcaag cagttggcga
    30361 ccttgacttt gctaacgacc gtactgccta catgatggaa tgtcctaatc tggtctttgt
    30421 agaggaacat cctgtccttc tctactgtcc acaaggattg gataagaaag ttctagacta
    30481 cgataatatc tttccaaata tgtataagat tggggcttcc tttgacccta aaaatgccaa
    30541 aatggtagat gtgtctcaac ttcaaaacat ggattacggt ttcgaagcct atgcaactca
    30601 agccttcaac gctcctgatg ggcgtgctct agcagttagc tggcttggtt tgccagatgt
    30661 ttcttaccca tctgaccgtt ttgaccacca aggaaccttc tctttggtca aggaactcac
    30721 tatcaaagac gacaagctct accagtatcc agtcgctgct attaaggacc ttcgtgcttc
    30781 tgaagaagcc ttctcaaacc gttcccaaac caagaacact tacgaacttg aactcaactt
    30841 ggaagctaat agccagagcg agattgtctt acttgctgat aaagaaggta agggactttc
    30901 aatcaacttt gaccttgtaa acggtcaagt gacagtggat cgtagccagg ctggagaaca
    30961 gtatgcccaa gaatttggga caactcgttc ttgccctatc gagaatcagg ctactactgc
    31021 tacaatcttc atcgataact ctgtctttga aattttcatc aataaaggag aaaaagtatt
    31081 ttctggtcgt gtcttcccac atgcggacca aaatggtatc ctgatcaaat ctggaaaccc
    31141 aactggaact tactatgaat tagattatgg tcgcaaaact aactgatgtc gccaaacttg
    31201 caggcgtcag tcctactacc gtttctcggg ttatcaataa aaaaggatat ctatctgaga
    31261 aaaccatcca aaaagtcaat gaagccatga gagaattagg ctataaaccc aacaacctgg
    31321 ctcgtagtct gcaaggaaaa tcagctaagt taatcggctt gattttcccc aatatttcca
    31381 atgttttcta tgcagaattg attgataaat tggaacacca actcttcaaa aatggttaca
    31441 agaccatcat ctgcaacagt gaacatgatt ctgagaagga acgcgaatac atcgaaatgt
    31501 tggaagccaa tcaggtagac ggcatcattt ctggtagtca caacctagga atcgaagact
    31561 acaatcgtgt gacagcgccg attatttcct ttgaccgaaa cctatcgcca gacatccctg
    31621 ttgtctcctc tgacaactat gctggtgggg ttcttgctgc ccaaaccttg gtcaagacag
    31681 gtgcccagtc tatcatcatg attacaggga atgacaattc caattcgcca accggactgc
    31741 gccacgctgg ttttgcatcc gtactcccaa aagctcctat tatcaatgtt tccagtgact
    31801 tttctcccgt cagaaaagaa atggaaatca agaatatctt gacccgggaa aaaccagatg
    31861 ccatttttgc ttcggatgat ttgacagcta ttctggtcat taaaatcgct caagaattgg
    31921 gcatttctgt cccaaaagag ctcaaggtca tcggctatga tgggacctac tttatcgaaa
    31981 attactaccc tcaattggct actatcaagc aacctttgga agagattgct tgtctcacta
    32041 ttgatcttct cttgcaaaag attgaaggca aggaagtcgc cacaactggt tacttcttac
    32101 cagttacgct attaccagga aaaagtattt aaacacaaga aaactcagac cgattcgtct
    32161 gagtttttat gatcttaaat tttcgagata gcgctgggct gtctctaggt taaaggtttt
    32221 atctgagatg aggcgctcta ctaggggagc aacttcagat tcactagccc cagctaggag
    32281 agctagggat ttggcctgta gtttcatgtg gccttgctgg atgcccgtac ttaccaaggc
    32341 tttgagggct gcaaaatttt gagcaagacc gatggacacg ataatctggg ctaattctct
    32401 ggcagaagga tttcctagta gatcatgact gagagctaca cgtgggttga ggccgataga
    32461 gccaccctta gtcgctacag gcatgggcag ggtcatctca ccgaccaatt cttctctttc
    32521 aaggtccagc gtccagcagc taagaccttg atagcgtcca tctcgactgg caaaggcatg
    32581 ggccccagct tcgatggcac gccagtcatt accagtggca atcaaaatcg catcaatacc
    32641 attaaaaatt cctttattat gagtagcagc tcggtaagga tcagcctgcg caaactgact
    32701 agccaacgca attttctccg caatctctcg tccttgatcc ttttggcggc tcaagtagcg
    32761 aaaggcgatg cgacagcttg cagtcaccag agaatcagtc gcgtagttgg acaggattcc
    32821 catgagactc tgtccctgac tgagttcttc taagactggt ttcaaggctt ccagcatggt
    32881 gttgagcata ttggcaccca tggcttcctg ggtatcgaca tgaatataaa caacgagaaa
    32941 gtctggttcg ccttttatct gctcgacatg cagatcacgc gccccacctc cacgtttaac
    33001 gatagaagga taggcttgat tggcaagctc caagagctcc gctttcttgc tggcaatctt
    33061 ctcttgcgct agtttaggat tagcaacttg ataaagggct acctgcccaa tcatctggcg
    33121 ctgatggact tgtgcagtaa aaccacctgc acgcttgatg attttgctgg catagctggc
    33181 cgccgcaacc acagagggtt cttctgtcac atagggaacg gtgtattcct gaccgttgac
    33241 aagtacctcc ggaaccagcg aataaggcag agaaaaagtt cccaccacat tctcactcag
    33301 ctggtctgcc acagtcacac tcatctgttc atccttctcc agactagctt gtctctcagg
    33361 actaaggagc gcctgagctt ttagcagctc gaggcgctct tggtatgatt ttttagaaaa
    33421 tccattccaa cttatcttca ttatttttca accttgctat aacggcgttg gtggtcaaga
    33481 atttcaacca aggcaaaatc ttgattttca tagccagcaa actgggcaga ttttgtttca
    33541 tccaagttta cttcctcaaa aaagaccttt tcatagtctg caacggatag ggcagttcgt
    33601 tggttgagct tgttcaagcg gtctttatcc aaataagctt catatccttc aaccaattca
    33661 ccactgaaga actcagccac agctccactt ccgtaactat aaagggcgat tttatcccca
    33721 gctttcaagc tatctgtatt ttccaagaga gacaaaagtc caaggaaaag tgaacctgtg
    33781 tagatattcc ccaccttttg actgtagaga atagactggt caaaatgctt ttgtaagagg
    33841 tcttttttct cttgaggcag gctcttatcc atgatttttt tcaagccttt tagcgctaat
    33901 ttaggataag gcaagtggaa acaaacagcc gcaaaatcat ccagagtaag ctggtagcgt
    33961 ttttgatatt caagccaagt cgttttcaaa ctatccaagt attgttgggt agaataaaca
    34021 ccatttacat aaggagttgt cgagtaattt ggtcgccaga aatccatgat gtcacgggtc
    34081 tgagctacat tgtcattatt aaaggccatc atgcgtggat tttgtgtaat caacatagct
    34141 acacttccag caccttgagt tggttctccc ggagtttcaa taccatattt ggcaatatca
    34201 ctggcaatga ccaatacctt ggactccgga gaattttcca catgcaattt ggcataatgg
    34261 agggcagctg tcgccccata gcaggcttct ttaatctcga aactacgagc aaagggctgg
    34321 atgcccagca agccatgcac aaagacggcc gcagccttac tctggtcaat tcctgactcg
    34381 gtagccacaa tgaccatgtc aacttcttgt ctttcttgct cagttaaaat agagtcacta
    34441 gcactggccg ccaaggtcac gatgtcctca gttaggggcg caatactcaa ttccttgagt
    34501 aagagtcctt tacttaattt ttcagggtca attcccctcg cttctgctaa gtcttgtaat
    34561 ttcaagacat attgactggt cgcaaaacca atcttatcaa taccgattgt catatttacc
    34621 tctgttttat cattcatgta aaaaatcgtt ctatactatt ttatcacaaa tggcagtaaa
    34681 agagagaaaa aagacttgat tcaccaaatc aagcctctta ttggtcatca ttttaaagaa
    34741 tgattagttg ctagagagtt catcgatata agtagcttta taagctccat tcacagttat
    34801 cagctcctgg aggatcaaat ttcctgagta agtccttccc atctcatcta caaatttttg
    34861 ataaaactga ctggtcggaa tttctctgac atccttatca aatgtcttgt caagtgtttt
    34921 actaaccttc tcagcaatca attgatgctc ttgccatcca ctttgaaact ctgagcccga
    34981 actagaaacc atgactggga taaacaacaa ggtcagtaga cttacagaca ataaggaaaa
    35041 tagtagactt cctgcaaaac tagaatccta gttcatgatt gataatacca acaatcaaat
    35101 tcattcgtaa tccgaagcgt ttacgatgat ttcgataggt tgttgaaatc attttaaacg
    35161 tttttacttt ggcaaagatg ttctcaacct tgcttc
//
LOCUS       DAVMMR010000019        34331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMMR010000019 DAVMMR010000000
VERSION     DAVMMR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.019"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            434..886
                     /locus_tag="VYS74_000878"
     CDS             434..886
                     /locus_tag="VYS74_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3788938.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGEVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VYS74_000879"
     CDS             905..2242
                     /locus_tag="VYS74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV3788939.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VYS74_000880"
     CDS             2321..3205
                     /locus_tag="VYS74_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3788940.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VYS74_000881"
     CDS             3205..4038
                     /locus_tag="VYS74_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3788941.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4057..6150
                     /gene="nanB"
                     /locus_tag="VYS74_000882"
     CDS             4057..6150
                     /gene="nanB"
                     /locus_tag="VYS74_000882"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV3788942.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6163..7266
                     /locus_tag="VYS74_000883"
     CDS             6163..7266
                     /locus_tag="VYS74_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3788943.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7440..8138
                     /locus_tag="VYS74_000884"
     CDS             7440..8138
                     /locus_tag="VYS74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3788944.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8157..9674
                     /locus_tag="VYS74_000885"
     CDS             8157..9674
                     /locus_tag="VYS74_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3788945.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9799..11127
                     /locus_tag="VYS74_000886"
     CDS             9799..11127
                     /locus_tag="VYS74_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3788946.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11379..12245
                     /locus_tag="VYS74_000887"
     CDS             11379..12245
                     /locus_tag="VYS74_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3788947.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12261..13100
                     /locus_tag="VYS74_000888"
     CDS             12261..13100
                     /locus_tag="VYS74_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3788948.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13372..13824
                     /locus_tag="VYS74_000889"
     CDS             13372..13824
                     /locus_tag="VYS74_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3788949.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            13966..14274
                     /locus_tag="VYS74_000890"
     CDS             13966..14274
                     /locus_tag="VYS74_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788950.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14284..14499
                     /locus_tag="VYS74_000891"
     CDS             14284..14499
                     /locus_tag="VYS74_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3788951.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKSPKEKRKNRFVHGRDL"
     gene            14557..15201
                     /locus_tag="VYS74_000892"
     CDS             14557..15201
                     /locus_tag="VYS74_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3788952.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEVLQKGLILASFNFP
                     WFLLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            15374..16291
                     /locus_tag="VYS74_000893"
     CDS             15374..16291
                     /locus_tag="VYS74_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV3788953.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEILKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVIEEDRPVVEAAAALIRETKERFL"
     gene            16309..17193
                     /locus_tag="VYS74_000894"
     CDS             16309..17193
                     /locus_tag="VYS74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3788954.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17209..18060)
                     /locus_tag="VYS74_000895"
     CDS             complement(17209..18060)
                     /locus_tag="VYS74_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3788955.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18260..20317
                     /gene="pbp2b"
                     /locus_tag="VYS74_000896"
     CDS             18260..20317
                     /gene="pbp2b"
                     /locus_tag="VYS74_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV3788956.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKILETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     AYMVQTALGLMGQTYQPNMFVGTSKLESAMGKLRSTFGEYGLGSATGIDLPDESTGFV
                     PKDYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20328..20924
                     /gene="recR"
                     /locus_tag="VYS74_000897"
     CDS             20328..20924
                     /gene="recR"
                     /locus_tag="VYS74_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV3788957.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21100..22143
                     /locus_tag="VYS74_000898"
     CDS             21100..22143
                     /locus_tag="VYS74_000898"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV3788958.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSQTPGQEDRLMTNETIDWDKQVAPSAIYEEGVVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPGLIERLVDL
                     AKESFDKREAHLI"
     gene            22227..23600
                     /locus_tag="VYS74_000899"
     CDS             22227..23600
                     /locus_tag="VYS74_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV3788959.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGQDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23587..24198
                     /locus_tag="VYS74_000900"
     CDS             23587..24198
                     /locus_tag="VYS74_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3788960.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24289..24993
                     /locus_tag="VYS74_000901"
     CDS             24289..24993
                     /locus_tag="VYS74_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV3788961.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25212..26585
                     /gene="ftsA"
                     /locus_tag="VYS74_000902"
     CDS             25212..26585
                     /gene="ftsA"
                     /locus_tag="VYS74_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV3788962.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26602..27861
                     /gene="ftsZ"
                     /locus_tag="VYS74_000903"
     CDS             26602..27861
                     /gene="ftsZ"
                     /locus_tag="VYS74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV3788963.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27866..28537
                     /locus_tag="VYS74_000904"
     CDS             27866..28537
                     /locus_tag="VYS74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV3788964.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28547..29086
                     /locus_tag="VYS74_000905"
     CDS             28547..29086
                     /locus_tag="VYS74_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV3788965.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29086..29349
                     /locus_tag="VYS74_000906"
     CDS             29086..29349
                     /locus_tag="VYS74_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV3788966.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29346..30131
                     /locus_tag="VYS74_000907"
     CDS             29346..30131
                     /locus_tag="VYS74_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV3788967.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30140..30928
                     /gene="divIVA"
                     /locus_tag="VYS74_000908"
     CDS             30140..30928
                     /gene="divIVA"
                     /locus_tag="VYS74_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV3788968.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31276..34068
                     /locus_tag="VYS74_000909"
     CDS             31276..34068
                     /locus_tag="VYS74_000909"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV3788969.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPVGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     ICFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10529 a         6068 c         7484 g        10250 t
ORIGIN      
        1 ttagaaaaac gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgatttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatactgaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgctttgagg ttgtagataa gactgacgta tataatccaa ggtgaagcga
      301 ctgtggtttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccgttta ggaacttatg tgggagtaaa
      481 tccacatttt gcaacattaa tagatttttt agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggagccttct ttgaaaccca ccaaaaatat ttggatattc atttagtgct
      661 ggaaaacgaa gaagccatgg ctgtgacgtc gccggaaaat gtaagcgtta cccaagagta
      721 tgatgaagag aaggatattg aattatacac aggggaagtg gaacagttag ttcatttgag
      781 agctggcgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggttcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaact ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaagt ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaatga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgagctga
     2281 aaagctctag cccaatcttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttttct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gttggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtattgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcaattt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca accctcattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct atattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata agattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaaatga ataaaagagg tctttattca
     4081 aaactaggaa tttctgttgt aggcattagt cttttaatgg gagttcccac tttgattcat
     4141 gcgaatgaat taaactatgg tcaactgtcc atatctccta tttttcaagg aggttcatat
     4201 caactgaaca ataagagtat agatatcagc tctttgttat tagataaatt gtctggagag
     4261 agtcagacag tagtaatgaa atttaaagca gatgaaccaa actctcttca agctttgttt
     4321 ggcctatcta atagtaaagc aggctttaaa aataattact tttcaatttt catgagagat
     4381 tctggtgaga taggtgtaga aataagagac gcccaaaagg gaataaatta tttattttct
     4441 agaccagctt cattatgggg aaagcataaa ggacaggcag ttgaaaatac actagtattt
     4501 gtatctgatt ctaaagataa aacatacaca atgtatgtta atggaataga agtgttctct
     4561 gaaacagttg atacattttt gccaatttca aatataaatg gtatagataa ggcaacacta
     4621 ggagctgtta atcgtgaagg taaggaacat tacctcgcaa aaggaagtat tgatgaaatc
     4681 agtctattta acaaagcaat tagtgatcag gaagtttcaa atattccctt gtcaaatcca
     4741 tttcagttaa ttttccaatc aggagattct actcaagcta actattttag aataccgaca
     4801 ctatatacat taagtagtgg aagagttcta tcaagtattg atgcacgtta tggtgggact
     4861 catgattcta aaagtaagat taatattgcc acttcttata gtgatgataa tgggaaaacg
     4921 tggagtgagc caatttttgc tatgaagttt aatgactatg aggagcagtt agtttactgg
     4981 ccacgagata ataaattaaa gaatagtcaa attagtggaa gtgcttcatt catagattca
     5041 tccattgttg aagataaaaa atctgggaaa acgatattac tagctgatgt tatgcctgcg
     5101 ggtattggaa ataataatgc aaataaagcc gactcaggtt ttaaagaaat aaatggtcat
     5161 tattatttaa aactaaagaa gaatggagat aacgatttcc gttatacagt tagagaaaat
     5221 ggtgtcgttt atgatgaaac aactaataaa cctacaaatt atactataaa tgataagtat
     5281 gaagttttgg agggaggaaa gtctttaaca gtcgaacaat attcggttga ttttgatagt
     5341 ggctctttaa gagaaaaaca taatggaaaa caggtcccta tgaatgtttt ctacaaagat
     5401 tcgttattta aagtgactcc tactaattat atagcaatga caactagtca gaatagagga
     5461 gagagttggg aacaatttaa gttgttgcct ccgttcttag gagaaaaaca taatggaact
     5521 tacttatgtc ccggacaagg tttagcatta aaatcaagta acagattgat ttttgcaaca
     5581 tatactagtg gagaactaac ctatctcatt tctgatgata gtggtcaaac atggaagaaa
     5641 tcctcagctt caattccgtt taaaaatgca acagcagaag cacaaatggt tgaactgaga
     5701 gatggtgtga ttagaacatt ctttagaacc actacaggta agatagctta tatgactagt
     5761 agagattctg gagaaacatg gtcgaaagtt tcgtatattg atggaatcca acaaacttca
     5821 tatggcacac aagtatctgc aattaaatac tctcaattaa ttgatggaaa agaagcagtc
     5881 attttgagta caccaaattc tagaagtggc cgtaagggag gtcaattagt tgttggttta
     5941 gtcaataaag aagatgatag tattgattgg aaataccact atgatattga tttgccttcg
     6001 tatggttatg cctattctgc gattacagaa ttgccaaatc atcacatagg tgtgctgttt
     6061 gaaaaatatg attcgtggtc gagaaatgaa ttgcatttaa gcaatgtagt tcagtatata
     6121 gatttggaaa ttaatgattt aacaaaataa aggagaaaaa acatggttaa atacggtgtt
     6181 gttggagcag ggtattttgg agctgaattg gctcgctaca tgcaaaagaa tgatggagca
     6241 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
     6301 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc
     6361 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
     6421 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg
     6481 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
     6541 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
     6601 gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa
     6661 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
     6721 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
     6781 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgccttg
     6841 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
     6901 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
     6961 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
     7021 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
     7081 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagcccca
     7141 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
     7201 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta
     7261 aaataaattt tggtattctc ctatttatag gtcgacttgc tcctctgaaa gtacttttag
     7321 aggagctgtt tgactttgct agtttttgaa actgaaatct attatactac aaactattga
     7381 aagcgtttta attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga
     7441 tgccacagat tagcaaagaa gccttgattg agcaaatcaa agatggaatc atcgtttctt
     7501 gtcaggctct tcctcatgaa ccgctttata cagaagcggg aggggtgatt cccttgctgg
     7561 tcaaagcggc tgagcaaggt ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca
     7621 aggaaattaa ggaagtcact aaacttccaa tcattgggat tatcaaacgt gattatccac
     7681 ctcaggaacc cttcatcacg gctactatga aagaagttga tgaattggca gaactggaca
     7741 tcgaggtgat tgctctggat tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag
     7801 agttcattcg tcaggttaag gagaaatatc ctaatcagct tttgatggct gatactagta
     7861 tcttcgaaga agggctagca gctgtagaag caggaattga ctttgtcgga acaaccttat
     7921 caggctacac atcctacagt ccaaaagtag acggtccaga ttttgaattg attaagaaac
     7981 tctgtgatgc cggtgtagat gtcattgcag aaggaaaaat tcatacacca gaacaagcca
     8041 aacaaatcct tgaatatgga gtgcgaggca tcgttgttgg tggcgccatt actagaccaa
     8101 aagagattac agaacgcttc gttgctagtc ttaaataaga tgtgagaggg agttttatgt
     8161 ttaaagtttt acaaaaagtt ggaaaagctt ttatgttacc tatagctata cttcctgcag
     8221 caggtctact tttggggatt ggtggtgcac tttcaaaccc aaccacgata gcaacttatc
     8281 caatactaga caatagtatt tttcaatcaa tattccaagt aatgagctct gcaggagagg
     8341 ttgtattcag taatttgtca ctacttctct gtgtgggatt atgtattggc ttagcgaaac
     8401 gagataaagg aaccgctgcg ttagcaggag taactggtta cttagttatg actgcaacga
     8461 tcaaagcttt ggtaaaactt tttatggcag aaggatctgc aattgatact ggagttattg
     8521 gagcattagt tgtcggaata gttgccgtat atttgcacaa ccgatataac aatattcaat
     8581 taccttccgc tttaggattc tttggaggtt cacgcttcgt tcctattgtt acatcgttct
     8641 cttctatctt gattggcttt gtcttctttg ttatttggcc acctttccaa caacttcttg
     8701 tttctacagg tggatatatt tctcaggcgg gtccaattgg aacttttcta tatggatttt
     8761 taatgagact ttctggagca gtaggcttac atcatataat ttaccctatg ttttggtata
     8821 ctgaacttgg tggtgttgaa actgttgcag gacaaacagt ggttggagct caaaaaatat
     8881 tttttgctca attagccgat ccggcccatt ctggattatt tacagaagga acaaggtttt
     8941 ttgcaggtcg tttctcaaca atgatgttcg gtttaccggc tgcctgttta gcgatgtacc
     9001 atagtgttcc taaaaatcgt cgtaaaaaat acgcgggttt gttttttgga gttgctttaa
     9061 catcttttat taccggtatt acagaaccaa ttgaatttat gtttctattc gtcagtccgg
     9121 ttctatatgt tgttcacgca ttccttgatg gtgttagctt ctttattgca gacgtcttaa
     9181 atatttcaat aggaaacaca ttttcaggag gtgtaatcga tttcacttta tttggaattt
     9241 tgcaggggaa cgctaagacg aattgggttc ttcagattcc aattggactt atttggagtg
     9301 ttttgtatta tattattttt agatggttca ttactcaatt caacgttcta acgccaggac
     9361 gaggagaaga agtagattct aaagaaattt ctgaatccgc agattcaact tcaaatactg
     9421 cagattattt aaaacaggat agcctacaaa ttatcagagc cttgggtgga tcaaataata
     9481 tagaagatgt agatgcttgt gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag
     9541 ttgataaagc acttttaaaa caaattggtg cagttgatgt cttagaagtg aagggtggca
     9601 ttcaagcaat ctatggagca aaagcaatct tatataaaaa tagtattaat gaaattttag
     9661 gtgtagatga ttaagtactt actgacttaa taaaaaacag aggagagtga tggatgagta
     9721 ggatgaaatg aaatcgtata caagaaataa agaactcatt atccaagttg gatacgctta
     9781 ttacatagga gaatacaaat gaaatttaga aaattagctt gtacagtact tgcgggtgct
     9841 gcggttcttg gtcttgctgc ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca
     9901 ggtggtgacg gtgccaaaac agaaatcact tggtgggcat tcccagtatt tacccaagaa
     9961 aaaactggtg acggtgttgg aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca
    10021 aacccagata taaaagtgaa attggaaacc atcgacttca agtcaggtcc tgaaaaaatc
    10081 acaacagcca tcgaagcagg aacagctcca gacgtactct ttgatgcacc aggacgtatc
    10141 atccaatacg gtaaaaacgg taaattggct gagttgaacg acctcttcac agatgaattt
    10201 gttaaagatg tcaacaatga aaacatcgta caagcaagta aagctggaga caaggcttat
    10261 atgtatccga ttagttctgc cccattctac atggcaatga acaagaaaat gttagaagat
    10321 gctggagtag caaaccttgt aaaagaaggt tggacaactg atgattttga aaaagtattg
    10381 aaagcactta aagacaaggg ttacacacca ggttcattgt tcagttctgg tcaaggggga
    10441 gaccaaggaa cacgtgcctt tatctctaac ctttatagcg gttctgtaac agatgaaaaa
    10501 gttagcaaat atacaactga tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc
    10561 tggattaaag acaatttgat caataatggt tcacaatttg acggtggggc agatatccaa
    10621 aactttgcca acggtcaaac atcttacaca atcctttggg caccagctca aaatggtatc
    10681 caagctaaac ttttagaagc aagtaaggta gaagtggtag aagtaccatt cccatcagac
    10741 gaaggtaagc cagctcttga ataccttgta aacgggtttg cagtattcaa caataaagac
    10801 gacaagaaag tcgctgcatc taagaaattc atccagttta tcgcagatga caaggagtgg
    10861 ggacctaaag acgtagttcg tacaggtgct ttcccagtcc gtacttcatt tggaaaactt
    10921 tatgaagaca aacgcatgga aacaatcagc ggctggactc aatactactc accatactac
    10981 aacactattg atggatttgc tgaaatgaga acactttggt tcccaatgtt gcaatctgta
    11041 tcaaatggtg acgaaaaacc agcggatgct ttgaaagcct tcactgaaaa agcaaacgaa
    11101 acaatcaaaa aagctatgaa acaatagttc ttagttattc tataaaaagt agttttttaa
    11161 agaacctaag agtgtatacc cccttttccc tctacacaga tagtgtaaga aaagggggct
    11221 tttgtttaaa atgtaagaaa ctgtcacgaa attaaaatga agttcttaca taagcgaatc
    11281 ataaaaaatt tcattttgat tttaaaacag ttcaagaaag tcaaaaaatt attctatttg
    11341 aaagagaggt gccgactgtg aaagtcaata aaatccgtat gcgggaaaca gtgatttcct
    11401 acgctttcct agcaccagta ttattcttct ttgtcatctt tgtgttggct ccgatggtga
    11461 tgggcttcat tacaagtttc tttaactact caatgactaa atttgagttt gtaggcttgg
    11521 ataactatat ccgtatgttt aaagatcctg tctttacaaa atctctgatt aacacagtta
    11581 ttttggttat tggatctgta ccagttgttg ttctattctc actctttgta gcatctcaga
    11641 cctatcatca aaatgtcatt gccagatcct tctaccgttt cgtcttcttc cttcctgttg
    11701 taacgggtag tgttgccgtg acagttgttt ggaaatggat ttatgaccca ctatcaggga
    11761 ttctaaactt tgtccttaag tccagccaca tcatcagcca aaacatttct tggttgggag
    11821 ataaaaactg ggcattgatg gcgattatga ttattctctt gaccacttca gttggtcagc
    11881 ccatcatcct ttatatcgct gccatgggga atattgacaa ttcactggtt gaagcggcgc
    11941 gtgttgatgg tgcaactgag tttcaagttt tttggaagat taaatggcca agccttcttc
    12001 caacaactct ttatattgca atcatcacaa caattaactc attccagtgt ttcgccttga
    12061 ttcagctttt gacatctggt ggtccaaact actcaacaag taccttgatg tactaccttt
    12121 acgaaaaagc cttccaattg acagaatacg gctatgccaa cacaattggt gtcttcttgg
    12181 cagtcatgat tgctatcgta agctttgttc aatttaaagt acttggaaac gacgtagaat
    12241 actaaagaaa ggagacagct atgcaatcta cagaaaaaaa accattaaca gcctttactg
    12301 ttatttcaac aatcattttg ctcttgttga ctgtgctgtt catctttcca ttctactgga
    12361 ttttgacagg ggcattcaaa tcacaacctg atacaattgt tattcctcct cagtggttcc
    12421 ctaaaatgcc aaccatggaa aacttccaac aactcatggt gcagaaccct gccttgcaat
    12481 ggatgtggaa ctcagtattt atctcattgg taaccatgtt cttagtttgt gcaacctcat
    12541 ctctagcagg ttatgtattg gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta
    12601 tctttatcgc tgctatggcg cttccaaaac aagttgtcct tgtaccattg gtacgtatcg
    12661 tcaacttcat gggaatccac gatactctct gggcagttat cttgcctttg attggatggc
    12721 cattcggtgt cttcctcatg aaacaattta gtgaaaatat tcctacagag ttgcttgaat
    12781 cagctaaaat cgacggttgt ggtgagattc gtaccttctg gagtgtagcc ttcccgattg
    12841 tgaaaccagg gtttgcagcc cttgcaatct ttaccttcat caatacttgg aatgactact
    12901 tcatgcaatt ggtaatgttg acttcacgta acaatttgac catctcactt ggggttgcga
    12961 ccatgcaggc tgaaatggca accaactatg gtttgattat ggcaggagct gcccttgctg
    13021 ctgttccaat cgtcacagtc ttccttgtct tccaaaaatc cttcacacag ggtattacta
    13081 tgggagcggt caaaggataa tactctgcga aaatctcttc aaactacgtc agcttcacct
    13141 tgccatactt aagtattgcc tgcggttagc ttcctagttt gttcttcaat tttcattgag
    13201 tataggaaaa tcaatctatc aagatacaga agtatatttt atagatttag agaatataga
    13261 ggttataagt gtctacaaaa tggagggtat gcagttactt tatgaagttt tgtcagacac
    13321 ttataaactt aagaatggtt ttagttaact atcagaaacg aaggaaagag tatgattttt
    13381 gacgatttga aaaacatcac cttttacaaa gggattcatc ctaatttaga caaggctatc
    13441 gactatctct accaacatcg taaggattct ttcgaattag gaaagtatga tattgatgga
    13501 gataaagtct ttctagttgt tcaggaaaat gtcctcaatc aagctgaaaa tgatcaattt
    13561 gagtatcata agaactatgc agatttgcat ttgctggtag aaggacatga atattcgagc
    13621 tacggttcac gtatcaaaga cgaggcagta gcattcgacg aagcgagtga cattggcttt
    13681 gttcattgtc atgaacacta cccactcttg ttgggttatc acaattttgc gattttcttc
    13741 ccaggtgagc cacatcagcc aaatggttat gcaggcatgg aagaaaaggt tcgaaaatat
    13801 ctctttaaaa ttttgattga ttaaaaatag gatgaattgt ttttttgtaa agctttgata
    13861 atactctacc atgaaattga tctttgtgag gtagagaaat gagaataaaa tatttaaaaa
    13921 ttggtatctt cttgtcagtc tgtgtatgct acaagagcta gtttcttgga tggacaggga
    13981 attacagttg atgagatggc ttggataatt aggggcattg tgaatgcatt gattggtaga
    14041 tacataaaat taggtactta tgcggctaag tatggtatta gtatggcacg ctcgatctta
    14101 agtagggcag ctgcaactgc agcagcaaga gtaggattat cgaccaagat ttctggatgg
    14161 attttacgag tagctgtgaa tgtagctgat gtatatggta attttgccaa caatattgct
    14221 gcagcttggg atgcacatga taaaatccct aacaatggtc gtataaactt ttaaaatgcg
    14281 agaatgaaag cactttgtat ttttttattg aatatgttag cttggacagt gcttgcaatg
    14341 ataattcgtg gagggctaga tggatttgat gagcatactt ggagtactat tttaattgct
    14401 tcgctgttcg gggtatatga ttataagccc atagataaaa atagaaaaag tccaaaagaa
    14461 aaaagaaaaa atagatttgt tcatggtagg gatttatgaa agctttactg acaaaaaaga
    14521 aaacagttta caaagaaaaa tgatggagga gcaaacatgg cacaaaaagg agtaagcctt
    14581 atcaaggcag catttgatac agacaacttt ctcatgcgtt ttagtgagaa agtcttggac
    14641 atcgtgacag ccaatcttct ttttgtcgtc tcttgtttgc ccatcgtgac gattggagta
    14701 gctaaaatca gcctctacga gaccatgttc gaagttaaga agagcagacg ggtgcctgtt
    14761 tttaaaatct atctaagatc tttcaagcaa aatctgaaac taggtcttca gctgggttta
    14821 atggagttag gaattgtgtt tcttaccctt tcagatctct atcttttctg gggtcaaaca
    14881 gctctgccct tccaattgct gaaagccatt tgtttaggta ttctgatttt tcttactatc
    14941 gtgatgctgg ctagttaccc tatcgcggca cgttatgacc tatcttggaa agaagtgtta
    15001 caaaaagggc ttatcttggc aagttttaac tttccttggt tcttactcat gttagccatt
    15061 cttgtcctca ttgtgatggt tctttatctg tctgccttca gtctactctt aggtggctca
    15121 gccttcctac tttttgggtt tggacttctt gcttttttac aagtgggagt gatggaaaag
    15181 ctatttgcta agtatgagta aggggtgcaa ttatttctga aactactttc aaaggctcca
    15241 aacgctattc tataagcgag aaactaaaat ctaagtgtct ataagatatt gaaagccttt
    15301 tcggtaaagt gtatactaaa gaagtaaaga aaaatctgct ttagcagaaa ataaaaataa
    15361 taggagaaaa tctatgtcag atttgaaaaa atacgaaggt gtcattccag ccttctacgc
    15421 atgttatgat gatcaaggag aagtaagccc agaacgtacg cgtgccttgg ttcaatactt
    15481 cattgataaa ggtgttcaag gtctttatgt caatggttct tctggtgaat gtatctacca
    15541 aagcgttgaa gatcgcaagt tgattttgga agaagtcatg gcggtagcca aaggtaaatt
    15601 gaccattatt gcccatgttg cttgcaataa tactaaagat agtatggaac ttgctcgcca
    15661 tgctgaaagc ttgggagtag atgctattgc aacgattcca ccaatttatt tccgcttgcc
    15721 agaatactca gttgccaaat actggaacga tatcagttct gcagctccaa acacagacta
    15781 cgtgatttac aacattcctc aattggcagg ggttgctttg actccaagcc tttacacaga
    15841 aatattgaaa aatcctcgtg ttatcggtgt gaagaactct tctatgccag ttcaagatat
    15901 ccaaaccttt gtcagccttg gtggagaaga ccatatcgtc tttaatggtc ctgatgagca
    15961 gttcctaggt ggccgcctca tgggtgctaa ggctggtatc ggtggtactt atggtgctat
    16021 gccagaactc ttcttgaaac tcaatcagtt gattgcggat aaggacctag aaacagcgcg
    16081 tgaattgcag tatgctatca acgcaatcat tggtaaactc acttctgctc atggaaatat
    16141 gtacggtgtc atcaaagaag tcttgaaaat caatgaaggc ttgaatattg gatctgttcg
    16201 ttcaccattg acaccagtaa ttgaagaaga tcgtccagtt gtagaagcgg ctgcagcctt
    16261 gattcgtgaa accaaggagc gcttcctcta atctaaaagg aggtatttat gacatactac
    16321 gttgcaattg atatcggtgg aactaacatc aagtatggtt tggttgatca agaggggcaa
    16381 cttcttgaat cacatgaaat gccaacagag gcgcataagg gtggacctca tatcttacaa
    16441 aagaccaaag atatcgtagc tagttattta gaaaaaggcc cagtagcagg tgttgccata
    16501 tcttctgctg ggatggtgga tccggataag ggtgagattt tctatgctgg gccgcaaatc
    16561 cctaactacg caggcaccca gttcaaaaag gaaatcgaag aaagctttac tattccttgt
    16621 gagattgaaa atgatgtcaa ctgtgcaggt cttgctgagg cagtatctgg ttcaggcaag
    16681 ggagcaagtg tgacactttg cttgaccatt ggaaccggta tcggtggttg cttgattatg
    16741 gataggaaag tcttccatgg ttttagtaat tcagcctgtg aagtcgggta tatgcatatg
    16801 caggatggag cttttcagga tctcgcttct acaacagctt tagtgaaata tgtagctgaa
    16861 gcccatggag aagatgttga tcagtggaat ggccgacgta tctttaagga agctactgaa
    16921 ggaaacaaaa tctgcatgga aggtattgac cgtatggttg actatctagg aaaaggtctg
    16981 gcaaatattt gctacgttgc caatccagaa gtggtcattc ttggtggtgg tatcatgggg
    17041 caagaggcta tcctcaaacc taagatccgc acagccttga aagaggcttt ggtaccaagt
    17101 ttagcagaaa aaacacgatt agaatttgcc catcaccaaa atacagcagg gatgttgggt
    17161 gcatattatc attttaagac aaaacaatcc tagtttggct cagccaaact aggattttct
    17221 tacacgtttt tgtctacgat agccgttgag ttttttattt tcccagtagc tattaaagat
    17281 tttttccttg ctttcgcgat tgatttccaa aaagtaggca taaatcaaat cgataaagaa
    17341 gagcatagga agttgagcgg atattcgttg gatataggag ggttggctgt gggtggctac
    17401 aagaacagtc tctgtatagg tctggctatc tttattggga acacttgtaa agagtacagt
    17461 ctttgccccc atctccttgg catctaatag actatctaaa atagaaggag ttgagcctga
    17521 aagtgagaag cccagtacta gacaattttc atccatgatg ctggttgtcc aggcaaagcc
    17581 gtcttggtct gtcaaagctt cgcagaccac acctaatcgc ataaaacgta atttcatttc
    17641 acgggcgacg aggccagaac tccctgttcc aaagaagtag atacgctcag catcttcgat
    17701 tagctgggca attcgttcta gttggatttc gtcaatcaag tcttgtgttt gttccctcat
    17761 attgctataa cttctgagga ctcgtttggt cagtggactg tgcttggaga cttggttggc
    17821 ttgattttct gcctgatgtt ggtattggaa aataaattct cggtagccag taaagccaca
    17881 ctttttagca aagcgggtca aagcagcttg agaaatatgt aatttttggg tgacttgttg
    17941 agaagataaa tcatctgtaa tcgtttcagc ttgcaaaaaa tagcgagcga tttcttgctc
    18001 taggtctgtc atttcttcaa aatgtgaatc aatgatagtt gcgatatctg gtttgtccat
    18061 agggaaagct cctttacatg agtcatactg gaagactaga tcagggaata gtcacacttc
    18121 attataacac ataatataag gatagataaa taaaaacgca tctctgtttt aaaaacgaaa
    18181 aaatcgaaaa agcttctctc ttttccataa ttttctgctt aaattgtggt acaattaaga
    18241 gtaagatttt aagttagaaa tgagactgat ttgtatgaga aaatttaaca gccattcgat
    18301 tccgattcgg cttaatttat tgttttcaat tgtcattttg ctctttatga ccattattgg
    18361 tcgtttgttg tatatgcagg ttttgaacaa ggatttttac gaaaaaaagc tagcctcagc
    18421 tagtcagacc aaggtcacaa ccagttctgc tcgtggggaa atctatgatg ctagtggaaa
    18481 acctttggta gaaaatacgt taaagcaggt tgtttccttt acgcgtagta ataaaatgac
    18541 ggctacagac ttaaaagaaa tagctaaaaa gttactgact tatgtgagca tcagttcgcc
    18601 aaatttgaca gaacgccagc tggctgatta ctatttggct gatcctgaaa tctataaaaa
    18661 aacagtggaa gctctcccaa gtgagaaacg cttggattca gatggcaatc gcctatccga
    18721 atcagaactg tataacaatg cggtcgatag tgttccaaca agtcaactaa actatacaga
    18781 ggatgaaaag aaagaaatct atctttttag tcagttaaat gctgttggaa actttgcgac
    18841 aggaaccatt gcgacagatc ctctaaatga ttctcaggtg gctgttattg cctctatttc
    18901 aaaggagatg cctggcatta gtatttctac ttcttgggat cgaaagattt tggaaacttc
    18961 cctttcttct atagtaggga gtgtatccag tgaaaaagct ggtctcccag cggaagaagc
    19021 agaagcctat cttaaaaaag gctattctct aaatgaccgt gttggaacct cctatttgga
    19081 aaagcaatat gaagagacct tacaagggaa acgctcggta aaagaaatcc atctggataa
    19141 atatggcaat atggaaagtg tggatacaat tgaggaaggt agtaagggaa acaatatcaa
    19201 actgaccatt gatttggcct tccaagatag cgtggatgcc ttgctgaaaa gttatttcaa
    19261 ttcagagttg ggaaatggtg gagccaagta ttctgaaggt gtctatgcag tcgcccttaa
    19321 cccaaaaaca ggtgctgttt tatccatgtc agggatcaaa catgacttga aaacgggaga
    19381 gttgacgcca gattccttgg gaacggtaac caatgtcttt gttccaggtt cggttgtcaa
    19441 ggcggcgact atcagctcag gttgggaaaa tggagtcttg tcaggaaatc agaccttgac
    19501 agaccagtcc attgtcttcc aaggttcagc tcccatcaat tcttggtata ctcaggctta
    19561 cggttcattc cctattacag cagtccaagc tctggagtat tcatctaatg cctatatggt
    19621 ccaaacagcc ctaggtctta tggggcagac ctatcaaccc aatatgtttg tcggcaccag
    19681 caagctagag tctgctatgg ggaaattgcg ttcaaccttt ggtgaatatg gtttgggttc
    19741 tgcgaccggg attgacctac cagatgaatc tactggattt gttcccaaag actatagctt
    19801 tgctaattac attactaatg cctttgggca gtttgataac tatacgccca tgcagttggc
    19861 tcagtatgta gcaactattg caaatgatgg tgttcgtgtg gctcctcgta ttgttgaagg
    19921 catttatggc aataatgata agggaggcct gggcgacttg attcagcaac tgcaaccgac
    19981 agagatgaat aaggtcaata tatccgactc cgatatgagc atcttgcacc aaggttttta
    20041 tcaggttgct catgggacta gtggattgac aactggacgt gccttttcaa atggtgcctt
    20101 ggtatccatt agcggaaaaa cgggtacagc cgaaagctat gtggcagatg gtcagcaagc
    20161 aaccaatact aatgcggtgg cctatgcccc atctgataat ccccaaatcg ctgttgcagt
    20221 ggtctttcct cataatacca atctaacaaa tggtgtagga ccttccattg cgcgtgatat
    20281 tatcaatctg tatcaaaaat accatccaat gaactagaaa ggaatttatg ctttatccaa
    20341 cacctattgc taagctgatt gacagttatt ccaagttacc aggtatcggg attaagacgg
    20401 ctacgcgtct ggccttttat acgattggga tgtctgatga cgatgtcaat gaatttgcaa
    20461 aaaatctcct ttctgctaag agagaattga cctactgttc tatttgtgga cgtttgacag
    20521 acgacgatcc ttgttctatc tgtaccgatc cgactcgtga ccagacaacg attttggtgc
    20581 tagaggatag tcgtgatgtg gcggccatgg aaaatatcca agaataccat ggactctatc
    20641 atgtcctgca tggccttatt tctcctatga atggtatcag tccagatgat atcaatctca
    20701 agagtctcat gactcgtctg atggatagtg aggtttcaga agtaatcgta gcaaccaatg
    20761 ctacagcaga tggagaagcg acttccatgt atctttcacg tttgctcaag ccagctggta
    20821 tcaaggttac gcgcctggcg cgaggtctcg ctgtgggagc agatatcgag tatgcggacg
    20881 aagtgaccct cttacgggct attgaaaatc gaacggagtt gtaagtgtag acaaatttac
    20941 gaactccatt catttataaa aaatcaaaga ggctgaaaat cgttcctatc ggcctttttt
    21001 tgtatagtgt gatgagtaga atcgggttca agttttaaaa aaacaagcaa atatgatata
    21061 ctaaagagcg agtattctag tagaattagg acaaataata tgaaacaaac gattattctt
    21121 ttatatggtg gacggagtgc agagcgtgaa gtctctgtcc tttcagctga gagtgtcatg
    21181 cgtgcggtta actacgaccg tttcacagtc aagactttct ttatcagcca gtcaggtgac
    21241 tttatcaaaa cgcaagaatt tagtcagact ccggggcaag aagaccgtct catgaccaat
    21301 gaaaccattg attgggataa gcaggttgca ccaagtgcta tttacgaaga gggagtagtg
    21361 gtttttccag tccttcacgg tccgatggga gaagatggct cagttcaagg attcttggaa
    21421 gttttgaaaa tgccgtatgt cggatgtaat atcttgtcat ctagccttgc catggacaaa
    21481 atcacgacta agcgtgtttt agaatctgct ggcattgcac aagttcctta tgtggctatc
    21541 gttgaagggg atgatgtgac tgctaaaatc gctgaagtgg aagaaaaatt ggcttatcca
    21601 gtcttcacta agccgtcaaa catggggtct agtgtcggta tttctaagtc tgaaaaccaa
    21661 gaagaactcc gtcaagcctt aaaacttgcc ttccgatatg acagccgtgt cttggttgag
    21721 caaggagtga atgcccgtga aattgaggtt ggcctcttgg gtaactacga tgtcaagagc
    21781 acgctacctg gagaagttgt caaggacgtt gccttttatg actacgatgc caagtatatt
    21841 gataacaaga ttactatgga tattcctgcc aaaatcagtg atgatgtggt ggctgtcatg
    21901 cgtcaaaatg cagaaacagc cttccgtgcc attggtggcc ttggtctatc tcgttgcgat
    21961 ttcttctata cagataaggg agagattttt ctcaacgagc tcaataccat gccaggtttt
    22021 acccagtggt ctatgtaccc actactttgg gacaatatgg ggatcagcta cccaggacta
    22081 atcgagcgtt tggttgacct tgccaaggaa agctttgaca agcgcgaagc gcatttgata
    22141 taaaatgaaa gagagggtag aagccagaac catcactgca cggtgactag agttctcgga
    22201 cttcagccct ttttaaagga gtagaaatga aattaacaat ccatgaaatt gcccaagttg
    22261 ttggagccaa aaatgatatc agtatctttg aggacaccca gttagaaaaa gctgagtttg
    22321 atagtcgttt gattggaact ggagatttat ttgtgccact taaaggtgcg cgtgatggcc
    22381 atgactttat tgaaacagcc tttgaaaatg gtgcagcagt aaccttgtct gagaaagagg
    22441 tctcaaatca tccttacatt ttagtagatg atgttttgac agcctttcaa tccctagcat
    22501 cctactatct tgaaaaaacg actgttgatg tctttgctgt tacaggttca aatggcaaga
    22561 caacgactaa ggatatgttg gcgcatttac tgtcaacaag atacaagacc tacaaaacac
    22621 aaggcaatta caataatgag attggccttc cttacacagt tctccacatg cctgaaggaa
    22681 cagaaaagtt ggtcttggag atgggacagg atcacttggg cgatattcat ctcttgtctg
    22741 aattggctcg tccaaaaaca gccatcgtga ccttggttgg agaagcccat ttggcctttt
    22801 tcaaagaccg ttcagagatt gctaagggaa aaatgcaaat tgcagacgga atggcttcag
    22861 gttccttgct tttagcgccg gctgacccta tcgtagagga ctatttgcca actgataaaa
    22921 aggtggttcg ttttgggcaa ggggcagagc tggaaattac tgacttggtt gagcgcaaag
    22981 atagtctgac cttcaaggcc aatttcttag agcaagccct tgatttgcca gtaactggca
    23041 agtacaatgc gacaaatgct atgattgcat cctatgttgc cttgcaagaa ggagtttcag
    23101 aggagcaaat tcgtttggcc ttccaagatc ttgaattgac gcgtaaccgt accgagtgga
    23161 agaaagcagc caatggagca gatatcctgt cagatgttta caatgccaat ccaactgcta
    23221 tgaaactgat tttagagact ttctctgcca ttccagccaa tgaaggtggc aagaaaattg
    23281 cagtgttggc ggatatgaag gagcttggtg accagtctgt tcaacttcat aatcagatga
    23341 ttttgagcct ttctccagat gtgcttgata ccgtgatttt ctatggacaa gatattgctc
    23401 agttggctca attggccagt caaatgttcc caatcggcca cgtttactac ttcaagaaaa
    23461 cagaagacca ggatcaattt gaagacctag tcaagcaggt caaggaaagc cttggagccc
    23521 atgaccaaat cctgctcaaa ggctctaact ctatgaatct agccaagttg gtagaaagtt
    23581 tagaaaatga agacaagtga ttttgtcaag tatttgcaaa gaatgattgc cattacagat
    23641 actggcttaa cctttacaaa agatccgttt gaccgtgagc gctacgaaga cttgcgaagt
    23701 ctgttatctg aaatgttgaa tcaagcatca gaccttgatt ccgaagaagt ggcagaagtc
    23761 ttgaagccaa cttctgctta tgcgactccc ttaatggacg tccgtgcttg gattgttgag
    23821 gatgagaaga tttgtctggt taggggacaa ggagaggata gttgggcttt gccaggtggc
    23881 tttggtgaag tcggctattc tccaacagaa aatattctca aggaaattga agaagaaacc
    23941 ggttttaaag ccaaagttga aagactgcta gctgtttttg ataccaatcg tttccaacta
    24001 cagagcaaac agtatacaaa gtttgttttt ggatgtaagc ttcttgatgg acaattccaa
    24061 gaaaatcaag aaattgctga ccttcaattt tttgccattg accaactgcc gaacttatct
    24121 gaaaaacgca ttaccaagga gcaaatagag cttctttggc aggtttatca aggtcatagg
    24181 gggcaatatc ttgactaaga agatgattat cgtatttcta aatccatttt taacaactag
    24241 catggtataa taatatgcag gaaaattttg aattatgagg aagactagat gaatttatgg
    24301 gatattttct ttacgactca ggcaaccgag ccgcccaaat ttgacctttt ttggtatgtt
    24361 agcctattta cgctcttagc cttaaccttt tatacagccc atcgctatcg tgaaaagaag
    24421 gtttaccaac gatttttcca aatcttgcag actgttcagt taatccttct ttatggttgg
    24481 tactgggtca atcatatgcc actgtcagaa agcctaccct tttaccattg ccgtatggct
    24541 atgtttgtgg tactcttgct tcctggtcaa tccaaatata aacaatactt tgcattattg
    24601 ggaacatttg ggacattagc agcctttgtt tatccagtgc cagatgctta cccttttcca
    24661 catatcacca ttctatcctt tatctttggt catttagcac tcttggggaa ctctctagtt
    24721 tatctattga gacagtataa tgcgcgattg ctggatgtga agggaatttt tctcatgacc
    24781 tttgccctaa atgccttgat ttttgtggtc aatttggtga caggtggcga ttacggattt
    24841 ttgacaaaac cgccattggt tggggatcac ggtctagtag ctaattattt acttgtttca
    24901 attgtgctgg tagctactat cagtttgact aagaaaatct tagaattctt tttagctcaa
    24961 gaagcagaaa aaatgattgc aaaggaagct taacacagag ctttcttttt tgctcttaga
    25021 gagtttttac aagcagctta taaaataaga atttctgaat agacaaactc aaaaaatggc
    25081 tgggaaattt aggaaaaaag caagcacgat taaatttttt gtgttataat attttgtgaa
    25141 tagctatgcc tatgtttagc tatggaataa tacgaagtgc gaaacttgga agatagagag
    25201 gaagcgatgt aatggctaga gaaggctttt ttacaggtct agatattgga acaagctctg
    25261 tcaaggtgct tgtggccgag cagagaaatg gtgaattaaa tgtaattggc gtgagtaatg
    25321 ccaaaagtaa aggtgtaaag gatggaatta ttgttgatat tgatgcagca gcaactgcta
    25381 tcaagtcagc catttcccaa gcggaagaaa aggcaggcat ttcgattaaa tcagtgaatg
    25441 tcggcttgcc tggtaatctt ttgcaggtag aaccaactca ggggatgatt ccagtaacat
    25501 ctgatactaa ggaaattacg gatcaagatg ttgaaaatgt tgtcaaatca gctttgacaa
    25561 agagtatgac acctgaccgt gaagtcatta cctttattcc tgaagaattt attgtggatg
    25621 gtttccaagg gattcgtgac ccacgtggca tgatgggggt tcgccttgaa atgcgtggtt
    25681 tgctttatac aggacctcgt actatcttgc acaatttgcg taagacggtt gagcgtgcag
    25741 gtgttcaggt tgaaaatgtt atcatttcac cactagcaat ggttcagtct gttttgaacg
    25801 aaggggaacg tgaatttggt gctacagtga ttgatatggg ggcaggtcaa acgactgtcg
    25861 ctacaatccg taatcaagaa ctccagttca cacatattct ccaagaaggt ggagattatg
    25921 taactaaaga tatctccaag gttttgaaaa cctctcgcaa attagcggaa ggcttgaaac
    25981 tgaattacgg ggaagcctat ccgcctcttg caagcaaaga aaccttccaa gtagaggtta
    26041 ttggagaagt agaagcagtc gaagtgacgg aagcctactt gtcagaaatt atttctgcac
    26101 gaatcaagca catccttgaa caaatcaagc aagaattaga tagaaggcgt ctattggacc
    26161 tccctggtgg tattgtctta atcggtggga atgccatttt accaggtatg gttgagcttg
    26221 ctcaggaagt ctttggcgtc cgtgtcaagc tttatgttcc aaatcaagtt ggtatccgta
    26281 atccagcctt tgcgcatgtg attagtttat cagaatttgc gggtcaatta acagaagtta
    26341 atcttttggc tcagggagcg ataaaaggtg agaatgactt aagtcatcag ccaattagtt
    26401 ttggtgggat gctgcaaaaa acagctcagt ttgtacaatc aacgcctgtt caaccagctc
    26461 ctgctccaga agtagagccg gtggcgccta cagaaccaat ggcggatttc caacaagctt
    26521 cacaaaataa accgaaatta gcagatcgtt tccgtggatt gatcggaagc atgtttgacg
    26581 aataaagagg aaaaataaat tatgacattt tcatttgata cagctgctgc tcaaggggca
    26641 gtgattaaag taattggtgt cggtggaggt ggtggcaatg ccatcaaccg tatggtcgac
    26701 gaaggtgtta caggcgtaga atttatcgca gcaaacacag atgtacaagc attgagtagt
    26761 acaaaagctg agactgttat tcagttggga cctaaattga ctcgtggttt gggtgcagga
    26821 ggtcaacctg aggttggtcg taaagccgct gaagaaagcg aagaaacact gacggaagct
    26881 attagtggtg ccgatatggt cttcatcact gctggtatgg gaggaggctc tggaactgga
    26941 gctgctcctg ttattgctcg tatcgccaaa gatttaggtg cgcttacagt tggtgttgta
    27001 acacgtccct ttggttttga aggaagtaag cgtggacaat ttgctgtaga aggaatcaat
    27061 caacttcgtg agcatgtaga cactctattg attatctcaa acaacaattt gcttgaaatt
    27121 gttgataaga aaacaccgct tttggaggct cttagcgaag cggataacgt tcttcgtcaa
    27181 ggtgttcaag ggattaccga tttgattacc aatccaggat tgattaacct tgactttgcc
    27241 gatgtgaaaa cggtaatggc aaacaaaggg aatgctctta tgggtattgg tatcggtagt
    27301 ggagaagaac gtgtggtaga agcggcacgt aaggcaatct attcaccact tcttgaaaca
    27361 actattgacg gtgctgagga tgttatcgtc aacgttactg gtggtcttga cttaaccttg
    27421 attgaggcag aagaggcttc acaaattgtg aaccaggcag caggtcaagg agtgaacatc
    27481 tggctcggta cttcaattga tgaaagtatg cgtgatgaaa ttcgtgtaac agttgttgca
    27541 acgggtgttc gtcaagaccg cgtagaaaag gttgtggctc cacaagctag atctgctact
    27601 aactaccgtg agacagtgaa accagctcat tcacatggct ttgatcgtca ttttgatatg
    27661 gcagaaacag ttgaattgcc aaaacaaaat ccacgtcgtt tggaaccaac tcaggcatct
    27721 gcttttggtg attgggatct tcgccgtgaa tcgattgttc gtacaacaga ttcagtcgtt
    27781 tctccagtcg agcgctttga agccccaatt tcacaagatg aagatgaatt ggatacacct
    27841 ccatttttca aaaatcgtta agtaaatgaa tgtaaaagaa aatacagaac ttgtttttcg
    27901 agaagttgca gaggctagtc tgagtgctca tcgagagagt ggttcggtct ctgtcattgc
    27961 agttaccaag tatgtagatg taccgacagc ggaagccttg cttccgctag gtgtccatca
    28021 tatcggtgaa aatcgtgtag ataagtttct ggaaaaatat gaagctttaa aagatcgaga
    28081 tgtgacttgg catttgattg gtaccttgca aagacgtaag gtgaaagatg tcattcaata
    28141 cgttgattat ttccatgcat tggactcagt aaagttagca ggggaaattc aaaaaagaag
    28201 tgaccgagtc atcaagtgtt tccttcaagt aaatatttct aaagaagaaa gcaaacacgg
    28261 tttttcgaga gaggaactgc tggaaatctt gccagagtta gccagactag ataagattga
    28321 atatgttggt ttaatgacga tggcaccttt tgaggctagc agtgagcagt tgaaagagat
    28381 tttcaaggcg gcccaagatt tacaaagaga aattcaagag aaacaaattc caaatatgcc
    28441 tatgaccgag ttaagtatgg gaatgagtcg tgattataaa gaagcgattc aattcggttc
    28501 cacttttgtt cgtataggta catcattttt taagtaggag agaaccatgt ctttaaaaga
    28561 tagattcgat agatttatag attattttac ggaggatgag gattcaagtc tcccttatga
    28621 aaaaagagat gagcctgtgt ttacttcagt aaattcttca caggaaccgg ctctcccaat
    28681 gaatcaacct tcacagtcgg ctggcacaaa agagaacaat atcaccagac ttcatgcaag
    28741 acaacaggaa ttggcaaatc agagtcagcg tgcaacggat aaggtcatta tagatgttcg
    28801 ttatcctaga aaatatgagg atgcaacaga aattgttgat ttattggcag gaaacgaaag
    28861 tatcttgatt gattttcagt atatgacaga ggtgcaggct cgtcgttgtt tggactattt
    28921 ggatggagct tgtcatgttt tagctggaaa tttgaaaaag gtagcttcta ccatgtattt
    28981 gttgacacca gtgaacgtta ttgtaaatgt tgaagatatc cgtttaccag atgaagatca
    29041 acagggtgag ttcggttttg atatgaagcg aaatagagta cgataatgat ttttttaatt
    29101 cgtatgattt ataatgcagt ggatatttac tccctaattt tggtagcctt cgctgtcatg
    29161 tcttggtttc caggtgccta cgaatccagt ttaggtcgtt ggattgtagc gttggtaaaa
    29221 ccagtgcttg ctcccttgca acgcctgcct ttacagatag cgggtcttga tttatctgtt
    29281 tgggttgcga ttgttttggt tcgattttta ggagaaaacc tagtgcgttt tctggcgatg
    29341 ataggatgaa taaagggatt tatcagcatt tctccataga agatcgtcca tttcttgaca
    29401 agggaatgga atggataaag aaggtagaag atagctatgc tcctttttta actcctttta
    29461 tcaatcctca tcaggagaag ctattaaaga ttttggccaa aacctatggt cttgcttgta
    29521 gcagtagtgg ggaattcgtc tcgagtgagt atgttcgagt tttattatac ccagattatt
    29581 tccaaccaga gttttcagat tttgaaatat ctctccagga aattgtgtat tccaataaat
    29641 ttgaatattt aacgcatgct aagattttag ggacagtcat caatcaatta gggattgaac
    29701 ggaaactttt tggagatatc ctagtagatg aagaacgggc gcagattatg attaatcagc
    29761 agtttcttct cctctttcaa gatggattaa agaaaattgg tcgtatacct gtttcgctgg
    29821 aggaacgtcc tttcaccgag aaaatagata agctagaaca gtatcgagaa ctggatttat
    29881 ctgtgtctag ttttcgatta gatgttcttt tatcaaatgt tttgaaacta tctaggaatc
    29941 aagcaaacca gttgattgaa aagaaacttg tccaagtaaa ttatcatgtg gtagacaaat
    30001 cagattacac tgttcaagtt ggagacttga ttagtgtgag aaaatttggt cgcttgagat
    30061 tacttcaaga taagggacaa acgaaaaaag agaagaaaaa aataaccgtc cagttattat
    30121 taagtaagtg aggaatagaa tgccaattac atcattagaa ataaaggaca agacttttgg
    30181 aactcgattc agaggttttg atccagaaga agtcgatgaa tttttagata ttgtggttcg
    30241 tgattacgaa gatcttgtgc gtgcgaatca tgataaaaat ttgcgtatta agagtttaga
    30301 agagcgtttg tcttactttg atgaaataaa agattcattg agccagtctg tattgattgc
    30361 ccaggataca gctgagcgag tgaaacaggc ggcgcatgaa cgttcaaaca atatcattca
    30421 tcaagcagag caagatgcgc aacgcttgtt ggaagaagct aaatataagg caaacgagat
    30481 tcttcgtcaa gcaactgata atgctaagaa agtcgctgtt gaaacagaag aattgaagaa
    30541 caagagccgt gtcttccacc aacgtctcaa atctacaatt gagagtcagt tggctattgt
    30601 tgaatcttca gattgggaag atattctccg tccaacagct acttatcttc aaaccagtga
    30661 tgaagccttt aaagaagtgg ttagcgaagt acttggagaa ccgattccag ctccaattga
    30721 agaagaacca attgatatga cacgtcagtt ctctcaagca gaaatggaag aattacaagc
    30781 tcgtattgag gtagccgata aagaattgtc tgaatttgaa gctcagatta aacaggaagt
    30841 ggaaactcca actcctgtag tgagtcctca agttgaagaa gagcctctgc tcatccagtt
    30901 ggcccaatgt atgaagaacc agaagtagct ccagtgcatc cgacaggtcc aacaccagct
    30961 acagaaactg ttgattcagc accgggattt gaagcaccgc aagaatctgt tacaatttta
    31021 taagaaatat tctgagaaca atatcttatc cttatattcc cagcgagcag gagatggtgt
    31081 gagtcctgca ttccctatcg ataagattat cctctcaaac tatcaagtct gaatctagta
    31141 agatttgacg ttccccacgt tacgggataa gagggagaaa gactaaaact ttttccgaat
    31201 aaaggtggta ccacgatttt cgtccttttt ggaagtcgtg gtttttaatt tgttattatt
    31261 tataaaggag ataccatgaa actcaaagac acccttaatc ttgggaaaac tgaattccca
    31321 atgcgtgcag gccttcctac caaagagcca gtttggcaaa aggaatggga agatgcaaaa
    31381 ctttatcaac gtcgtcaaga attgaaccaa ggaaaacctc atttcacctt gcatgatggc
    31441 cctccatacg ctaacggaaa tatccacgtt ggacatgcta tgaacaagat ttcaaaagat
    31501 attattgttc gttctaagtc tatgtcagga ttttacgcac catttattcc tggttgggat
    31561 actcatggtc tgccaatcga gcaagtattg tcaaaacaag gtgtcaaacg taaagaaatg
    31621 gacttggttg agtacttgaa actttgccgt gagtacgctc tttctcaagt agataaacaa
    31681 cgtgaagatt ttaaacgttt gggtgtttct ggtgactggg aaaatccata tgtgaccttg
    31741 actcctgact atgaagcagc tcaaattcgt gtatttggtg agatggctaa taagggttat
    31801 atctaccgtg gtgccaagcc agtttactgg tcatggtcat ctgagtcagc ccttgctgaa
    31861 gcagagattg aataccatga cttggtttca acttcccttt actatgccaa caaggtaaaa
    31921 gatggcaaag gagttctaga tacagatact tatatcgttg tctggacaac gactccattt
    31981 accatcacag cttctcgtgg tttgacggtt ggtgcagata ttgattacgt tttggttcaa
    32041 cctgttggtg aagctcgtaa gtttgtcgtt gctgctgaat tattgactag cttgtctgag
    32101 aaatttggct gggctgatgt tcaagttttg gaaacttacc gtggccaaga actcaaccac
    32161 atcgtaacag aacacccatg ggatacagct gtagaagagt tggtaattct tggtgaccac
    32221 gttacgactg actctggtac aggtattgtc catacagccc ctggttttgg tgaggacgat
    32281 tacaatgttg gtattgctaa taatcttgaa gtcgcagtga ctgttgatga acgtggtatc
    32341 atgatgaaga atgctggtcc tgaatttgaa ggtcaattct atgaaaaggt agttccaact
    32401 gttattgaaa aacttggtaa cctccttctt gcccaagaag aaatctctca ctcatatcca
    32461 tttgactggc gtactaagaa accaatcatc tggcgtgcag ttccacaatg gtttgcctca
    32521 gtttctaaat tccgtcaaga aatcttggac gaaattgaaa aagtgaaatt ccactcagaa
    32581 tggggtaaag tccgtcttta caatatgatc cgtgaccgtg gtgactgggt tatctctcgt
    32641 caacgtgctt ggggtgttcc acttcctatc ttctacgctg aagatggtac agctatcatg
    32701 gtagctgaaa ctattgaaca cgtagctcaa ctttttgaag aacatggttc aagcatttgg
    32761 tgggaacgtg atgccaaaga cctcttgcca gaaggattta ctcatccagg ttcaccaaac
    32821 ggcgagttca aaaaagaaac tgatatcatg gacgtttggt ttgactcagg ttcatcatgg
    32881 aatggagtgg tggtaaaccg tcctgaattg acttacccag ccgaccttta cctagaaggt
    32941 tctgaccaat accgtggttg gtttaactca tcacttatca catctgttgc caaccatggc
    33001 gtagcacctt acaaacaaat cttgtcacaa ggttttgccc ttgatggtaa aggtgagaag
    33061 atgtctaaat ctcttggaaa taccattgct ccaagcgatg ttgaaaaaca attcggtgct
    33121 gaaatcttgc gtctctgggt aacaagtgtt gactcaagca atgacgtgcg tatctctatg
    33181 gatattttga gccaagtttc tgaaacttac cgtaagattc gtaacactct tcgtttcttg
    33241 attgccaata catctgactt taacccagct caagatacag tcgcttacga tgagcttcgt
    33301 tcagttgata agtacatgac gatttgcttt aaccagcttg tcaagaccat tcgtgatgcc
    33361 tatgcagact ttgaattctt gacgatctac aaggccttgg tgaactttat caacgttgac
    33421 ttgtcagcct tctaccttga ttttgccaaa gatgttgttt acattgaagg tgccaaatca
    33481 ctggaacgcc gtcaaatgca gactgtcttc tatgacattc ttgtcaaaat caccaaactc
    33541 ttgacaccaa tccttcctca tactgcggaa gaaatttggt catatcttga gtttgaaaca
    33601 gaagacttcg tccaattgtc agaattacca gaggctcaaa cttttgctaa tcaagaagaa
    33661 atcttggata catgggcagc cttcatggac ttccgtggac aagctcaaaa agccttggaa
    33721 gaagctcgta atgcaaaagt tatcggtaaa tcacttgaag cacacttgac agtttatcca
    33781 aatgaagtgg tgaaaactct actcgaagca gtaaacagca atgtagctca acttttgatc
    33841 gtgtctgagt tgaccatcgc agaaggacca gctccggaag ctgcccttag cttcgaagat
    33901 gtagccttca cagttgaacg tgctgctggt gaagtatgtg accgttgccg tcgtattgac
    33961 ccaacaacag cagaacgcag ctaccaggca gttatctgtg accactgtgc aagcatcgta
    34021 gaagaaaact ttgcggaagc agtcgcagaa ggatttgaag agaaataaga ttgaaaagtc
    34081 taggcaaaat tcaatttgag aagaaaagac aactaatttt atagtctatt aaacgcattg
    34141 tatcacgttt ttgaacacct gatatgatgc gttttttatt tattttaaaa atttgcgagg
    34201 tatgactttt tatactcaac aagaatcaaa gagaaactta gcaagctaac agtagtaaga
    34261 taaaatagga atttgatatt agggataaga ttggtaaata gtgtaatatt tttacaacaa
    34321 taaatttata t
//
LOCUS       DAVMMR010000020        32545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMMR010000020 DAVMMR010000000
VERSION     DAVMMR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.020"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(295..984)
                     /gene="rplA"
                     /locus_tag="VYS74_000910"
     CDS             complement(295..984)
                     /gene="rplA"
                     /locus_tag="VYS74_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV3788970.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1192..1617)
                     /gene="rplK"
                     /locus_tag="VYS74_000911"
     CDS             complement(1192..1617)
                     /gene="rplK"
                     /locus_tag="VYS74_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV3788971.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1740..2456)
                     /locus_tag="VYS74_000912"
     CDS             complement(1740..2456)
                     /locus_tag="VYS74_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV3788972.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2474..2935)
                     /locus_tag="VYS74_000913"
     CDS             complement(2474..2935)
                     /locus_tag="VYS74_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3788973.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(2937..3974)
                     /locus_tag="VYS74_000914"
     CDS             complement(2937..3974)
                     /locus_tag="VYS74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV3788974.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4072..5397)
                     /gene="brnQ"
                     /locus_tag="VYS74_000915"
     CDS             complement(4072..5397)
                     /gene="brnQ"
                     /locus_tag="VYS74_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV3788975.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5568..5660)
                     /locus_tag="VYS74_000916"
     CDS             complement(5568..5660)
                     /locus_tag="VYS74_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3788976.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5703..5855)
                     /locus_tag="VYS74_000917"
     CDS             complement(5703..5855)
                     /locus_tag="VYS74_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3788977.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(6015..6593)
                     /locus_tag="VYS74_000918"
     CDS             complement(6015..6593)
                     /locus_tag="VYS74_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV3788978.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6647..8047)
                     /gene="pepV"
                     /locus_tag="VYS74_000919"
     CDS             complement(6647..8047)
                     /gene="pepV"
                     /locus_tag="VYS74_000919"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV3788979.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8060..8665)
                     /locus_tag="VYS74_000920"
     CDS             complement(8060..8665)
                     /locus_tag="VYS74_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV3788980.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8836..9636
                     /locus_tag="VYS74_000921"
     CDS             8836..9636
                     /locus_tag="VYS74_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3788981.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9820..10653)
                     /locus_tag="VYS74_000922"
                     /pseudo
     CDS             complement(9820..10653)
                     /locus_tag="VYS74_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(10643..12490)
                     /gene="uvrC"
                     /locus_tag="VYS74_000923"
     CDS             complement(10643..12490)
                     /gene="uvrC"
                     /locus_tag="VYS74_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV3788982.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12530..13231)
                     /locus_tag="VYS74_000924"
     CDS             complement(12530..13231)
                     /locus_tag="VYS74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV3788983.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13310..14542)
                     /gene="murN"
                     /locus_tag="VYS74_000925"
     CDS             complement(13310..14542)
                     /gene="murN"
                     /locus_tag="VYS74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV3788984.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14547..15767)
                     /gene="murM"
                     /locus_tag="VYS74_000926"
     CDS             complement(14547..15767)
                     /gene="murM"
                     /locus_tag="VYS74_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV3788985.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15879..16658)
                     /locus_tag="VYS74_000927"
     CDS             complement(15879..16658)
                     /locus_tag="VYS74_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV3788986.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16727..18388)
                     /locus_tag="VYS74_000928"
     CDS             complement(16727..18388)
                     /locus_tag="VYS74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3788987.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18563..20785)
                     /gene="recJ"
                     /locus_tag="VYS74_000929"
     CDS             complement(18563..20785)
                     /gene="recJ"
                     /locus_tag="VYS74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV3788988.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21062..21821
                     /locus_tag="VYS74_000930"
                     /pseudo
     CDS             21062..21821
                     /locus_tag="VYS74_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21861..22625
                     /locus_tag="VYS74_000931"
     CDS             21861..22625
                     /locus_tag="VYS74_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV3788989.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22638..23315
                     /locus_tag="VYS74_000932"
     CDS             22638..23315
                     /locus_tag="VYS74_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3788990.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23325..23984
                     /locus_tag="VYS74_000933"
     CDS             23325..23984
                     /locus_tag="VYS74_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3788991.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKIKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24280..25470
                     /locus_tag="VYS74_000934"
     CDS             24280..25470
                     /locus_tag="VYS74_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV3788992.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25791..26672)
                     /locus_tag="VYS74_000935"
     CDS             complement(25791..26672)
                     /locus_tag="VYS74_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV3788993.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26813..28141)
                     /gene="vncS"
                     /locus_tag="VYS74_000936"
     CDS             complement(26813..28141)
                     /gene="vncS"
                     /locus_tag="VYS74_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV3788994.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(28138..28794)
                     /gene="vncR"
                     /locus_tag="VYS74_000937"
     CDS             complement(28138..28794)
                     /gene="vncR"
                     /locus_tag="VYS74_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV3788995.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28843..28926)
                     /gene="pep27"
                     /locus_tag="VYS74_000938"
     CDS             complement(28843..28926)
                     /gene="pep27"
                     /locus_tag="VYS74_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV3788996.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29106..30485)
                     /gene="vex3"
                     /locus_tag="VYS74_000939"
     CDS             complement(29106..30485)
                     /gene="vex3"
                     /locus_tag="VYS74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV3788997.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30537..31184)
                     /gene="vex2"
                     /locus_tag="VYS74_000940"
     CDS             complement(30537..31184)
                     /gene="vex2"
                     /locus_tag="VYS74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV3788998.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31197..32474)
                     /locus_tag="VYS74_000941"
     CDS             complement(31197..32474)
                     /locus_tag="VYS74_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3788999.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9195 a         7460 c         6052 g         9838 t
ORIGIN      
        1 cgattcgtcc tattcttatt tcattttatt ataattgata gtggtcgccc cagccagata
       61 ccttatccac tatataggcc ttattatcac gttcctgttc gtagagccac gatttcgcta
      121 tcccttcctc tgacgattct tatttccagc gtcagacttg ggaatcgcta ttgggttcac
      181 cggtagtagg tgcccaccga ggacttacac ctcagatata cgacatgccc gtcgtacatc
      241 aaaaaaagtt gagacatagt gtcttcaact tttttacatt actttaacta ctgattaaag
      301 tgagtttacg tcaactttga taccaacacc ttgagtagtt gtgatagtca agtttgttac
      361 gtaagttcct ttagctgtag ctggttttac tttttggatt gtttcgttga aagctttaaa
      421 gttttcaacc aatttttcag cttcaaatga tactttaccg atgattgctt gaacgttacc
      481 tgcacggtca gcacggtaag tgattttacc acctttagac tcttcaactg ctttagcaac
      541 atccattgtt acagtaccag ttttagggtt tggcatcaag ttacgtggtc caaggacacg
      601 tccaagacgt ccaacaagag ccatcatatc aggtgtagcg ataactacgt cgaagtccaa
      661 ccaaccgtcg ttgattttag caacaaggtc atcttcacca acaaagtctg caccagcagc
      721 ttttgcttct tcagcttttg caccacgtgc gaaaacaaga acacgtgaag ttttaccagt
      781 accgtttggc aataccattg ctccacggat ttgttggtca gcttttttaa cgtcgatgtt
      841 caagttgtaa gcaacttcta cagttgcatc aaattttgca aagttagttt cttttgcaag
      901 tgctacagct tcttctacac tgtatgcttt tgtgctgtcg attttctcaa gagcagcacg
      961 aagttgtttg ctttttttag ccattttcta ttctccttgt aagtggttca atcgattttc
     1021 atctcccacg tcacttctcg aaatgatgaa gtcttgcggg ttttcagtct actatttttc
     1081 tcttcctacg gccttaagct cccttgctgt atccaagtac aagcctcagt cacttgccta
     1141 gtatgaaaag caactaatag actgctgtga gattttctac tgtgttattg attagtcaac
     1201 aacagtgaat cccatagaac gagcagtacc ttcgatcata cgcattgcag actctacgtt
     1261 tgctgcgttc aaatctggca tcttagtttc tgcaatttct tgtacttgtg cacgagtaac
     1321 tgtagcaact ttagttttat taggtgtacc tgatcctttt tcaacacctg cagctttttt
     1381 caaaagaaca gcagctggtg gtgttttcgt aacgaaagta aatgatttat cttcgtaaac
     1441 tgagataaca actggaatga tcataccagc ttggtcagct gtacgagcgt tgaactcttt
     1501 tgtgaatccc atgatgttga taccagcttg accaagagca ggtccaaccg gtggagctgg
     1561 tgtagcttta ccagcaggga tttgcaattt tacaagtttt tcgacttttt tagccatttt
     1621 taaatcctcc tttgtggttt tggcggtaat tagagatttt tacctcccac aagtatgctt
     1681 tacacatacc tttccattat acactattta tctaaaaatg caagagaaaa ctttcatttt
     1741 taatcgacat aatcgccgcc ttcaaagcca tagtactctt ccaaactgag accacttgca
     1801 gcaatttctt tggcttcttt tccaacgtag cggagatgcc attcttctgc catgtagcca
     1861 gtctcctttt ccttgccttt aagatagcga acaacaaagc catagtcagc cgcatggtcc
     1921 aagagccact gggctgcctt ttcttcagta actaaatccc catctgtccc aatcaaatca
     1981 aaagcaagac ccgtctggtg ttcactgtaa cctgggcgtg ctgagtagcg atctgctgct
     2041 tccttaccat cttgattgac atagtcttgg tagagcttag cctgagtttc ataactccta
     2101 aacccactgt attggtcact gattggatag ccctcagcct gcatagctgc gattagtttg
     2161 agcaactctg ccttggctgt tggattttct cctggattgt aatcttttga caaaggataa
     2221 cgtttattag ctaggataat ctcatcgtat ttcccttgga tactataata gctcccttta
     2281 ttgctaactt ctgccttttt ctgactagaa gcttgagatt tgctacctac agttccttcc
     2341 ggttgactgc tttgctccgt tttttgagtg ttttcttcaa ctttggtttt ttcttgcgaa
     2401 caagctgcaa gggtcaatgc tagtaaacca gataaaatga catatctttt tttcatttct
     2461 ttcttcctct ctcttaggcc aaatacttct ccaattcatg agataagatt ttaatcatct
     2521 cttccagctc tggcgattgt acttggcaat cttctgccgc catttcttcc cagggcaccc
     2581 accagactgg gccacggcca ttcaaaccgt gactcttcat atcacctgct cttcggggaa
     2641 aaagatgcca atgagcgtgg gcatcgccat ttcctaggag ttcaatattc atcttttcag
     2701 cctcaaatgc tttggcgact gcttcttgga ctaaactcat ttcctctaaa aagcgaagtt
     2761 ttacagaagt ttccatatga tggagttctg tgacatgatc ttttgctaaa aataaggtat
     2821 agcccttaaa gtattggtag tcaccaataa caacatagcc tgtttctagt tcttttacaa
     2881 agtagggatt ttgcccagct ttaatgagtt cgattctctg acagattaag cacattctat
     2941 gataccgcct cgctcttaag ataagcatca accatacgct cagtcgctat tactgaatcg
     3001 atgtgggtgc gctcataaga gtgactggac tcaataccag ctccaagaag tgcatgcttg
     3061 acctctgcac ctgctgacat cgctgctgaa gcgtccgatc cgtaaaatgg atagatgtcg
     3121 agcttaaatg gaatatcttg ctctttagcc aaggcaacca agtgttgacg gaagtcatag
     3181 tgataaggac ctgaagcatc cttgacacag atagatactg tgtactcatc tgtctgctgg
     3241 tcatctccca tagctcccat atccacagcc agatattcta ctacctgagc aggaatgtta
     3301 gaattagcac catgtcctac ttcttcaaaa actgaaaaag caaaatgagt tgttactggc
     3361 aattcaatct tctctttctt ataaatacga agcaggttga gcaaaatcgc tgcgctgacc
     3421 ttatcatcca aatgacgaga cttgataaaa cctgtatcag tcacaacagt tcgtgtatcg
     3481 aagctgataa aatcaccgac ctcaatccca agagcacgag tttctttttc attgcttact
     3541 tttacatcca aacgcacttc catattgtcc tgcgtgcgtt ctgcagttcc tgcatccttg
     3601 tagacatggc aagaagtttg gtggatgagg atggttcctg ataccttttg acctgtgcta
     3661 gccacatgaa cggtacagtt ttcaccttca atcatgttcc aaggaaaacc accgatacgg
     3721 tccaatttaa gacgaccatc tggtttgaca gcacgaacaa tagcacccag cgtatccaca
     3781 tgggcagtca catagcgatg ttgctcatca ttttgacctt tgatggtcac attgacacca
     3841 cccttagctg tgcgaactgg ctggtaacca aaaccttcta gagtcttgac taaataattc
     3901 gaaatttcac gagtgaagcc cgttggcgat gcgatggctg tcagttcttt gatatattct
     3961 actgtttgat tcatttcttc acctctttcg ctacctatta taacacattt atcatcggat
     4021 aaaaaaagaa aatcactcct gtagacttgg ctacaaaagt gatttaagtg attattccat
     4081 ttcaaaaaca tcgctttctt gcttgtttgg tagaaccaat gagagcaaga ttccaacaat
     4141 ggctggcacc aaccatggga gagatgcctt ggcaaaagga agaacgctaa caagatttgc
     4201 aagaaactca accttaaacg agcttcctag tacgcttgca atggcaataa ctgtaaccac
     4261 agcaattgtc aactgcatac ctggttttga aagggcaaca aatttgttga caatgacaat
     4321 cataacgata gcaatcgtga ttgggtacaa gataaccagt actggaattg agtacttgat
     4381 aatcgcatca agacccaaat tggcaatagc aaatccaatc aaggtaaagg ctgtcgcata
     4441 aaccttgtag ctgatttgtg ggaagcgctc attaaagaac tcagctgttg acacaatcaa
     4501 accaacagtc gttgtgaagc aggttacggt aaccatagct gcaaggaaga gttgagctgt
     4561 tgagccaaag atttcttgag tggcttgtga caagatgtaa acacctggtg ttccaccctt
     4621 catcgcttca gctggtactg ggaaatgatt tccaagaaaa cctaaaccga tgtaaagagc
     4681 gctgaaggca agggcaacaa cgataccaac aacccaaata gttgaaatgt attctttctt
     4741 acttgaaaat ccaagttgtt tcaaggtttg aactgcgatt acgctaaagg ccactgaagc
     4801 aagggcgtcc aaggtattgt aaccttctag gaaacctgta ccaaaggcag aagcttgata
     4861 agcagctgaa gcagcttgag gacttgttcc accatatttg atagctccca gaacgaccaa
     4921 gataacaatc aaaattgcaa agactggcgt taaaacacgt ccaatgcggt ctaagatttt
     4981 tgatggatta agcgaaatca aataggctgc cgcaaaatac agaaccgtaa atacaatcaa
     5041 gccaagtcct ttatttgcat ccgacaaaag ggggctaatc cctacttcgt aagctgttgt
     5101 agcagtacgt ggggtagcaa agaatggacc gattgacaag taaagaactg agaggtaaag
     5161 agtcgcaaac caaggcgcta tcttcgttga aatctcgtag atatatcctt taggatttag
     5221 cgttccaata ataagggtca agacggcgat accaacgcct gaaaagacaa aacctgcgat
     5281 ggcaggaaga aaatgttctc cagatagagc acctagagaa ggcggaaaaa tcaagttccc
     5341 cgcaccaaaa aatattccaa acaggagcaa acctgttagg gtaccttttt tagccataaa
     5401 aatctccttc atatttataa aatactgacc tagtatacca cgattaggag tctgaaaaaa
     5461 gcatcacgag gcaaatatta aatgttttca attttcttaa aaataagaat tatctaaaaa
     5521 taaaactctc ctacccagtc cacaccaagt aggagaaatg caaaatttta aagttcttca
     5581 aaggctaaca tgccaccctg gacattgata acattataac cttgttctaa caggaattgg
     5641 caagcacgcg ccgatctcat tccagatttg caaataatat aatgcaagcg atctttgtcc
     5701 aattaatcat aactatcagc caattgacta agcggtaggt tgtgggcaca ttctaaatga
     5761 agagctgcaa actcatccac ttctctcaca tccactagag aaagctggtc gttttggtaa
     5821 agctggtaaa atgcgtcaaa ggctatttct ttcattcttt ttcttctaat actcttcgaa
     5881 aatctcttca aaccacgtca gcgtcgcctt accgtgcgta tgttaccgac ttcgtcagtt
     5941 ttatctgcaa cctcaaagct gtactttgag cagcctacga ctagcttcct agtttgctct
     6001 ttgattttca ttgactataa aatggtttta attctttttt tcaaatctgg cactacttct
     6061 gcctcaaacc aaggattttt ggccatccag atttgatttc gtggtgatgg gtgaactagc
     6121 ggaaaatagg ctggcagata gtctttatag tgtttcaccc tctccgttac cttcccactg
     6181 attttctcct gtaaatagta ggcttgggca tattgcccaa tcaagagggt taactgaata
     6241 tcaggcaatt cctgtaagag ctgcggatgc catttttctg caaaacctgt acgaggcgga
     6301 agatcacccg acttgccatg tcctggaaag tagaaatcca taggcaaaac agcaaaataa
     6361 cctgaattgt aaaaggtatc ttcatccaca cctagccagt cccgcaagcg gtcaccactt
     6421 ttatctttcc agtaaaggcc tgcttcttga gttttaagtc ccggagcctg accgatgata
     6481 ttgatgcgag cagtttttgg cgctgcaaag agaggctcaa tgccacgctc tgtatagctg
     6541 gcattctgtg aatccgccat gatagcctgt ttgattcttt cgatttgaga catctacttt
     6601 ccctccaaaa agaagtccaa gcataagatc tcagacttct atcgttttat ttgatcaatt
     6661 cgtaaatagc ttcggcatag attgctgctg ctcggaagag atcgtccaag gcgataaatt
     6721 cattggcttg gtgcatggta tcaattgagt ctgggaacat agcaccatag gcaactccgc
     6781 gctctaacaa gcgaccaaag gttccaccac cgatgacttg ttcatgacct ttaaagccag
     6841 tttgtttttc atagatattc aacaaggttt gcacaagtgg atcttccatt ggcacatagt
     6901 gaggcgtgtg accgtgttca gacaggctaa cagaaacaac tggcaagttt tcaaggattg
     6961 acttgatttg ttctggactt gttccttttg gatagcggat gttgagggca atggtattat
     7021 cagcacttgt ttcatcgaag cggaagacgc cggcattcat agaaagagca cccatctttt
     7081 catccacatg agcaatctta agattttcac cctcatgatc gttcaagaga attttacctg
     7141 cgatgtcaag gtagtctttg gctggaccag caaagccaaa ctgactgagg aagagggcaa
     7201 ggtaagttgc gccattgaca cctgaagcag gcatagcacc gtgggctgat ttaccaatga
     7261 tcgtcacctt gtatttgcca gcttcttctt ggagttctcc tctaagtttg tgttctgcaa
     7321 caaaggcatc tagtttagct tgcaagtcag ccaagtcacc tgaaacgact gctgttgctg
     7381 attctggtac catattttca cgtaaaccac ctgtaaagct gtgaagacgg gcaacacctg
     7441 tattttctcc tgcaaagtgg aggtattccg tgatatttcc tttttcacca ttgatgattg
     7501 gaaattcagc atctggtgag aaaccgaaat ctggtttggc aagtcctacg tgctcaaagt
     7561 agtagtccat gtctgcccag cctgattctt cgtctgttcc aacgatgaag cgaactttct
     7621 tagaagttgg aagacccaat tctttgatga ttttcaaacc atagtaacaa gctgttgtag
     7681 gacccttatc gtccgaagcc ccgcgcgcat aaaggcgacc atctttgata gttggtgtgt
     7741 aagggtctgt gtcccaaccg ctaccagcag gcaccacatc catatgggca aagattccga
     7801 gaacttcttc tccatcacca aactcaaaat gtcctgcata gttatcaaca ttcttagttg
     7861 ggtagccatc gcggtctgcg atttcaagga atttctccaa ggcttttact ggaccaggcc
     7921 caaatggatg ctgggcatca gccttgctgt catcacgttc tgaattgatt tccaaaaggc
     7981 taaacaagtc agccaagagg tcttctttgc gtttttctac ttctgctgta aaatcaattg
     8041 ctgtcatttt ttcttctttc tatcttttct cgatgatttc atctactggc aagcggtagc
     8101 ttggttccaa tttttcgtct gtataaccca ctgtgatcaa gagttctggg cggaaacggt
     8161 cttcgatttc caaaacttca ttaacttttg atttgtcaaa accaagaata atgttagaac
     8221 caattccttg gtctgtcaat gcaagaacca agttcatggc aaccaaacct gcattgagag
     8281 ctaggtagtc gctgacttgt tgttcattgt agcgtgcaaa ttcagctggc aaatttttca
     8341 tgaagtattg aagttgttct tctgagaaat tgttagcacc accaactcgg gcaatcttac
     8401 gagcgcgttt ggcaaggtct gtatctgtaa atagggcaat ggttacaggt gctgatgata
     8461 cctgctcaaa gtttgaacca taagccaact ttgccagttc agcatttttc tcacgaacaa
     8521 ctacaaattt ccaaggctgg ctgttgtggg cgcttggcgc caaggttgca atttcgatag
     8581 ccgtacgcac atcttttgga tcaacaggct tatcagtgaa atgcttggtc gcatgacgtt
     8641 ttttatttaa ttcaagaaat ttcataatcg atttcctttt ctaattctac tgcttccatt
     8701 ctaccataat ttgaaagagc ttgcagggat ttttcatgat taagtttttc tatcacagac
     8761 ataaaagata tatattggtt tatcaggaaa gtttctgata aactagcaag tactttacat
     8821 ttgaatggag acagtatgaa aaaatttagc ctattactag ctatcctacc atttttggtt
     8881 gcctgtggga atcaagctac acccaaagag actagcgctc aaaagacaat cgtccttgct
     8941 acagctggcg acgtgccacc atttgactac gaagataaag gcaatctgac aggcttcgat
     9001 atcgaagttc taaaggcagt agatgaaaaa ctcagcgact acgagattca atttcaaaga
     9061 accgcctggg aaagtatctt cccaggactt gattctggtc actatcaggc tgcggccaat
     9121 aatttgagtt acacaaaaga gcgtgctgaa aaataccttt actcgcttcc gatttccaac
     9181 aatcccctcg tccttgtcag caacaagaaa aatcctttga cttctcttga ccagatcgct
     9241 ggtaaaacaa cacaagagga taccggaact tctaacgctc aattcatcaa taactggaat
     9301 cagaaacaca ctgataatcc cgctacaatt aatttttctg gtgaggatat tggtaaacga
     9361 atcctagacc ttgctaacgg agagtttgat ttcctagttt ttgacaaggt atccgttcaa
     9421 aagattatca aggaccgtgg tttagacctc tcagtcgttg atttaccttc tgcagatagc
     9481 cccagcaatt atatcatttt ctcaagcgac caaaaagagt ttaaagagca atttgataaa
     9541 gcgctcaaag aactctatca agacggaacc cttgaaaaac tcagcaatac ctatctaggt
     9601 ggttcttacc tcccagatca atctcagtta caataacact cttcgaaaat ctcttcaaac
     9661 cacgtcagct ttatctgcaa cctcaaagct gtgctttgag caacctacgg ctagcttcct
     9721 agtttgctct ttgattttca tattgagtat aagatatatt aaaagcgatg ggatcagcca
     9781 atcgcttttt tagtttgcat tggttatttt aggaaactct tacaaaaaat caaaaaaatt
     9841 cttcacatcc tctacatacc tatgcttttc tattaagctt gctaatagtt ccagattgtg
     9901 cctctgatag ttatcttcac gacgtagttc ttcatacatt tcgataaagg gaaaaccctt
     9961 ggatttggca aattctaact cagcctcgta atcataagcg actttacgaa ataggatatt
    10021 taccaattcc ccatcttcaa cctctatcac ggcatactgg gcacggtgat tttttaacgc
    10081 ctcccaatta aaatagggca tgccaatcga ccctggattg atgatttgtt gcccttgact
    10141 accataacga agcaactact tgtgaacatg accataaact gccacgtccg tttccgcatc
    10201 tagcagttgg tcaaatttct ctgtatcatt ctcaactagc aagtcaccac catagttttt
    10261 gtcaggtaaa ttatgagaga tagaaaagcg caatccgtca atttctttct tttccagcaa
    10321 aggcaagctt cgtagccaga caatcgttgc aggatccatt cgctccatca aatactgtgt
    10381 catacgcaag agctgaactt cctgtgggtc ttctaagcca tattgcccat ctaaagcctc
    10441 aaggacacaa tcatcccaat tgcctcgaac acttgccgtg ataggaagtt cctttaacag
    10501 agcgaccaag tcatttgcac ctggaccagg gaggaaaatg tctcccatga gccaatattc
    10561 actggctcct tgagttttag catctgcaat cactgcttct agcgccgtcg cattgccatg
    10621 aacatctgat aaaattgcga ttttatggtt tattgtggtt tccttccgtt tggtaatcta
    10681 ctctttcttc cgccacttga agcaagattt ctgtttcctg cgggttcaac ttggtttgca
    10741 cagcctctgc tactgctctt ggtactccaa cttcgacaat ctcatccaca ctggcttcct
    10801 tgattttggt cagagacttg aagtgcctca tgagattttg cttgcgttta ggtcccagac
    10861 catcaatccc atccaattga gatgagaaag aatttttgga gcgcagttgg cggtggaagg
    10921 taatggcaaa gcggtggacc tcatcttgaa tgcgttggag gaggaaaaat tcctgagaat
    10981 tgcgagacaa gtccaccact tccagcggat ctccaaagag caattcatgg gtttggtgct
    11041 tatcattctt ttgcagacct gcaattggaa tatccaagcc cagttcttct tggatgactt
    11101 gcttagcaat attgacttga ccttgccccc catcaatcac aatcaaatct ggaggagtca
    11161 aagcctcacg ctgtactcga ccatagcgtc tgcgaatgac ctctcgcata ctggcatagt
    11221 cgtctggccc gactacggtc ttgattttat acttacggta atccttctta ctaggcttgc
    11281 cgttgacaaa gaccaccatg gctgaaacag gactagttcc catgatgttg gagttatcga
    11341 aggactcgat gcgaactggg gttgggattt gaagcaagcg tcctagattt tcaatagctc
    11401 cttgggtctt ctcgacagat ttctctagca ggttgaactt ctgctctagg ctaacacggg
    11461 cattttttat agctaaattg accagctgct ttttctctcc acgttgaggc ttaagaatct
    11521 tggaatccac caaggccttg acagcctctt cgtcaatatc ttgcggaatc agtacctcat
    11581 tgggaactag atgggatttt tcttgataga attgtcctac ataggtcaaa aaatcctcat
    11641 ctggatcatt gaagtagggg aagagattga catcgcgctc gatgagctta ccctgacgga
    11701 caaagaaaac ctgcacacac atccagccct tatccacata gtagccaaag acatcgcgat
    11761 tttggagatc cttagccatg acccgttgct tggttcgaag cgttccaata gcctgaatca
    11821 ggtcacggta ttccgccgca cgttcaaact ccatagtctg ggctgccgct gccattttgc
    11881 cctttagctc atcaatgatt ttgttatcct gccctttcag aaaatcagaa acctcctggg
    11941 ccatagactt gaaataaatc tcatccttct tacagatggt gtgggccatg cattgaccga
    12001 tatggtaata aaaacagacc ttagagggcg ggttggtaca cttacgaaaa gggaatatcc
    12061 gatccagcaa ccgcttgatt tcattggctg cccccacatc gggatagggt ccaaaataaa
    12121 gacctccgtc ctttttgacc tgacgagtga taatcaagcg tggatagcgc tcattggtga
    12181 ttttgatgaa aggataggac ttgtcatcct tgagcatgat attgtacttg ggcttgtttt
    12241 ccttgatcag gttgatttct aggagaagtg cctcaatatt agactccgta acaataaatt
    12301 caaaatccac aatttcagac accagagcct ctgtcttggt atcatgactt ccacgaaaat
    12361 aggaccgtac tcgattacgc agatttttag cctttcctac atagataatg gtgccatttt
    12421 tatccttatg aatgtagcaa ccagggctgg tcggcaagag ctctagtttt gatttaatca
    12481 agttgttcat agttccatta tagcaaaaaa agtagggaaa gatagtaccc tactcactag
    12541 tctcatatat ttttcggagg tttgcaacat cctcttcctg gataccataa aaggaacctg
    12601 ctggttgcat gacagacttc tcatctgtat ggtccaagcc aatcgcatga cctaactcat
    12661 gttctgccgt atggacaagg cgttcatagg agtagccata gtatggattg gacaaataat
    12721 aatgattcaa ccgcaccgtt acggacaaga attgccctgt taagagatta gtttgacttt
    12781 ccgcctctcc tgccacagga gtgcctccgt cgttcatctc cgtagccgta atatccgcct
    12841 tactagactc agtcacgagt tcaaagttaa aagcaccagt ttgattccag ttctggattg
    12901 cttctaaata agcctcttga aagcgtgaat ccatctgcgg atccaggtaa atacgagcag
    12961 aggcctgtgc ccaccttcct tcagaatgct ggtgactatc tgtctggctt aacttaggca
    13021 gttgccctgt tttctcccat tggtcaatac tttgttgacc aatttttact gaccgttctg
    13081 cttgctttag cgctccttga aaatctccgt tcagatacca gagaagtccg aaagcaagca
    13141 cacataagag cacaactatc caaaccagac gccaaaacaa acgccacaca aaagaaaaga
    13201 aagcccctat caaacgaaaa agccagcgca tgtctctcca cctttctagc aaataagaat
    13261 tattttacta aaagaagcta aaagaaatct aaatactgac tttttcatct taacgtccta
    13321 ctattttttt gagtaactgg atagctttgt attttaaagg agatggatgg taacggaaag
    13381 tacctgcttt gcgtacaata tagccattaa aattctgttt aaaacgcaaa acaccatcac
    13441 ttccatcaaa aatcccttga atgcctagga agttgtattt aggtattcca cgttttatgc
    13501 tttccaacat aacatatttt tgaagcaggg caggggcata gaacttatta aactcagtgt
    13561 aggaaccact aaagagataa gtcgtttcct gaggcatata aacaaataaa ctcccagcta
    13621 aaacaatatc ttcttctcca tatttttcaa tcaagtctcg cgcttctgct tttcgaactt
    13681 caaacgtttc aaattgacta gaatattctc tcagttgatt ttgttttttc tcagaatgag
    13741 gatttttact caaatcaagt cgcaacttgt ccaagttttc ttctagttta ctttgttcac
    13801 cttgcaattt gctcatatag tccgaaaaat ttaagcttgc tatgagaaac tccgcttgtt
    13861 ctccaaaagt atcataaaaa tgctcataat attctaaact tttatcacta tattctctac
    13921 gttcagaggt ttcttttgtt atattcttaa aaatcgatag ttcttcacgt tttaactttt
    13981 tcaaccgaat gccaaaggtt tcagcctttt tcaccaaggg tttacccttt ttgctaaaac
    14041 ttttaagcaa actcttttca gttaattcag ttaaatcttt atagtataac caatctggtt
    14101 ctccacctgg gtaacctgtt gttaagccat caaattgata acctaaatca gtcaaacctt
    14161 gaataatact ttttttctca gcatctattg gattaccttg gctatcaaaa gtttgataag
    14221 tttcataagg ttttacaagc aactctaata caccattttg cttggcatat tcttttaact
    14281 ctgcataaaa aactggaaga gcatcttgtt gggtataaat tggccccgaa ttgagttcca
    14341 tatgcagacc acccagcatg ggcaagctat aaaccagagc tgcaacttga atttctcctt
    14401 cttgtttcaa agcaagataa acaattcgag cccctctttt ttctagcaaa tcccccatct
    14461 ggacagattg cataaaggaa cgagaagaaa cctgatcaga ataagtctga aactcttctt
    14521 tcgtgagtgt tgttagtgcc atatacttac tttctatgtt tttttcttaa tgttttacgg
    14581 aaatcaagag caagtcttaa cagaggatag agaggatgag tgggcattgt aaattcaccc
    14641 aagtattctt caatcgttgg attaaatttt tccttaaaat gataaagtcc accattgaga
    14701 gagttttcaa caccacctaa attttgccag accatacctc gctcaaaggc atagcgagcc
    14761 gtttcatacc atgttaaaat tggtgcattg taacgtttaa aatcatcatc cataccagca
    14821 tatatattaa cagaggtagt accaaattcc aaactcaaag tagccgctaa aggaactctc
    14881 gcttgaccta catctatata ttcctgcaag aaggtcaatt cctctaacaa acgttctttt
    14941 tccttcttct gcgcttctac ttttgaagtt cgagtcgact cagtaaacgt ctcttccaag
    15001 gctctatttt tcgctaactg ttcttctaat tcttgcgaac gtttagaaac atccaaggtg
    15061 gccaaggtga tataggcctt gtccttaaaa ttatctaaca attttttata ataggcttca
    15121 ttcctcaaat gaatctcttt tcgcttctca gtttttttca tcaactccga aaatgaatct
    15181 aatagttcca gtccaccata ttgaatctca agccctttgt ttcgtgctgt tcgaatagcc
    15241 tgttttgttg acttggaaag tttatcttct tcaaaatttt ccttgtatat tttcgcctga
    15301 atacgaggtt gaatggtgtc tcccatttcc tccgtttttc ctgaccacct tactcccatt
    15361 tgttgcaaac tatcaataat agccagattt tcaggaaatt ctgtcttttc ctgattgatt
    15421 aaactttgag ataggcaaat acttgggtca aaagtcacaa aaaccgctct cttactgcga
    15481 gcataggact taatagactg aatggcaaaa ttcaagagtt ctttatcccc ataatccaat
    15541 ataggtcctc ttgggatgta aaacatttta tagcctagcg gaagagttct aatcaaaata
    15601 ctagctgtcg ccagtaattt ttcttccctg taaacaccaa acttctcatg ttgccaatta
    15661 gacttaactt cctcccaagc actactttgt aatacattgg ctaattcatg ttctttgaca
    15721 aactgatcat attctaatgt gggaatgcca atttgataac ggtacatttc ctactctctt
    15781 tcctccagta ttttattact attctactac tttcgtttga aaagtctagt gaaaacatat
    15841 tttattttag ttctactccc cctatgctga agttcaaact aagtcagtct ctcttctaat
    15901 ttgaaatcaa ttggtagggt tgttaaaaaa acttccaatt gtttttccca gtagtaccac
    15961 tcgtgagttc cagcactatg gctataggtc acatcaaaac ctagtttttt gagatttttc
    16021 actgcgagat tattggcttc gtacaagaaa tcctgttcgc cacaccacgc ccaaagtttg
    16081 gtctttttat ccgatttttt agccagactt tcaagagaat agggactagt tgtccagtct
    16141 ctaatctctc caaaaacacc tctccagtag gctggacttc ccagattttg actttcagga
    16201 gaaaagtttt gaaagctgag ggcacctgaa aaactagctg catgagaaaa acgatttgtc
    16261 gtaagagcca gtttgaagca gccgtagcct cccatagaaa gaccagcgat aaaggtcttt
    16321 tcacgcttgc tagtcatatt agggaagaag cgtttcagaa cctgtggcaa ttcctctgct
    16381 agagccatgt agtagtcaaa accatactgg gtatcggtgt accaaccatt gctggtattg
    16441 ggcataacaa cgatgagatt agttcctcga agcaagcgtt ctacattggt ccgcttaagc
    16501 caactattat gatttccaga catcccgtgc aaaaggtaca agacgggaat atcttcacac
    16561 tctggttctt ccactcgatt ggcatcaggg tagaggacat tcacccccca ctccatatcc
    16621 aatacttgtg agtaatactc gattttcatt actgccatga ttttctcctt ctatttttct
    16681 ataagaaaaa gccgaaactc gactttctct ctgtttattt caacgattat tttgcttcca
    16741 tgactactgg taaaatagct ggacgacgct tggtttggtc aaagagatac ttggtcaaat
    16801 tatctcgaac cttcccttta agatctgccc agtcaaagtc atctccttga agatactctt
    16861 ctaccgtttg gttaatcaat tctgaacttt cacggagaat atcgcgactc ttcttgagat
    16921 aaacaaatcc gcgcgtatga acacgagccc tagccacaat tttcttctca cgacggttga
    16981 ctgtaatagc cacgatgaaa attccatcct ctgacaagac cttacggtca cgaagaacaa
    17041 catttccaac atcaccaatg gcattcccat caatcaagat atctcctgct gaaaccgatc
    17101 cagctggaac aaagtctcca ttctcgtaag ccatggtcgt ccccttttta ggaatgaaga
    17161 tgcgttctgg caacatccca actgccatgg cagccttagc gtgagcatcc aactcacgat
    17221 actccccttg gacagggaag aggtacttag gttgcaaaag attgatcatc agctgcaaat
    17281 cacgcacatt tccgtgccct gatacatgta aactttgggt aatcaatttg acaaccccac
    17341 ctgcctgata aatcatattt tccacacgcg caacaaaggc ttctttagca atagacggag
    17401 ccgtagcaat atagaccagg tccccatcct tgatttctac ataacgatgg cgaccaatcg
    17461 acatcttacg aagtccattg ataggttcac ccatacgacc tgtctcaaga ataatcaact
    17521 catggtcttc aaagcgagac atatctttag gcttaatcaa gagaatttcg ttggctaaag
    17581 acaacttctt aagacgaatc gctgtgcgga cgatattttc aatatcaaat cctgtcaaga
    17641 cgatacgtcg acctgtttta tccgcagcgt caaaaatctg ctgaatacga gaaagattac
    17701 tggaaacagc tgcaacgatg atacgacctt cccagtcagc aatagtttgg gtaatttcat
    17761 ccctaacttc actttcacta gccacctgaa tattgctgtc tgcattggcc gaatcactga
    17821 ggagagccag gacgccgtca cgaccaatct ctgccaaacg agcaaaatca gttgcataag
    17881 attcactagc cgtttggtca aatttgaagt cacctgtata aacgatgctt ccttccgatg
    17941 tcttcaagac aattcccaga ctctctggaa cggagtaagt cgtagggaag aaggaaacca
    18001 ctgtcccacc aaaatcaatc tccgtattct catcaatgac atggaaatca ttaaatttct
    18061 taacggcatc atttcctttg acaaagagct ttgccaactc aatggtcaac tcagacccaa
    18121 atacaggaac tttagcctct gccaagagat aaggtagagc accaatggca tccgcatgcc
    18181 cgtgggtcaa gaaaacccca gcaatacggt cgctattttc aaaaaggtaa tccatgtttg
    18241 gaatcaccac atcgacccct aattgttcat tttcaggata ttttaaccct acattcaaaa
    18301 caaaaatgga ctctccaatt tcagcaatgt acatattttt tccattctca cgcacaccac
    18361 caagtgttgt taaactgata ttactcattt ttcctccaaa atcttagttt atcttgatta
    18421 tagggcaact ctaccctctt attctaaaag ttctaatact ttcagccgaa tcttttccta
    18481 ccattatacc atttttttga tgattttcaa aatgaaggtc tgttttcaaa aaagagttga
    18541 tttcccaact ctttcctaac ttctactctt tttccatcaa aaaatcataa atttcttgca
    18601 ccgtacccag cgccatcatt tcttggtctt taacggtttg tttgagattt tggtaaattt
    18661 gactttctcc tatctcccgc tttggcgcct ctttattgac tgtcatcaca ccatctttta
    18721 tcgtcacaaa gcctagttct tcaaatactt gaatcatctt gaccagcaag atttgttgaa
    18781 tattaagata tgcagccaaa tctttcagct tgtagcgaat atcaaactct gggaactggt
    18841 aaatagtctt gtacaatttg gcaaactgat ctctagtccc ataacctgtc agataatagg
    18901 ccttgtcaat atcatttttg aaatagacag cagagaaatg ctgttcctga aaaatggtct
    18961 tcagctgagt aatatcctct ggaatgtttt ttacgacaac agcttcacta gccgcaagat
    19021 ttggcagttc tccaggaaaa tccaagactg gaacaccttc tggcaagact gcattttttc
    19081 cacgaatgtt aaaaagttga acaccttcca ctcgcgcatc catcatcatc aactggaggg
    19141 cagtttggcc gttccattgg ttgacagaca atttaaccgc taactctaga ttcttggttt
    19201 gagaaaactc tgtcgcccat ctgccttgac caaaggctac cacttcaaaa ctcacctcac
    19261 ccttggaaat tttcagcttt agatgggcat tacctgcccc catagtacga gcactttcga
    19321 cctgaaaatt cttgatataa aaaataggtt tctgattatc cattccaaaa ggagctaaac
    19381 gttcaaaact tttgaccgtt tccaagctaa gtgcctccaa atccaactct tcatctaggt
    19441 ttaacttatt cttgccacca gcatctgcac ctttttcacg aacataatct tccaaaatct
    19501 gagataaatc tgagagttgc tcaacttcca gcgtcatacc cgctgcacct gcatgacctc
    19561 caaaggcgat gaagaggtct cgatagggat ccagagcttc aaaaatatcg accgcttcca
    19621 cactacgagc actgcccttg gcacgaccgt cttctatatt aagaacaatg actgtctgtc
    19681 ccaattcttc caataaacga ccagccacga ttcctagaac cccaggattc cagccttcct
    19741 tggccaagac ctgaaccttc ttctcaggat ccaccatggt cttggcttct tcatagatag
    19801 actgaacgat ttccttgcgc tcttcgtttt tctggtgaat cataagggca atctcatgcg
    19861 cttcctcatc atcaaatcca gtcaacaaat caatggcagg attgggatca tccaagcgac
    19921 ccaaggcatt caaacgagga gcaatctgga aaccaaccgt ttcttctgtt acttcgttag
    19981 cagcaatccc agccatgtcc agcatttctt gcagaccaat gcgctgggta tgacccaaca
    20041 tttccagacc atattgaact aagatacgat tttcatccgt cagactcacc atatctgcaa
    20101 tagttccaat agcgaccaaa tcaagcaatt ccacttgcac ttcttctaac agggcacaag
    20161 ccaacttgaa agcaactcca caaccagcca aatatttaaa aggataatcc gcatctggat
    20221 gttcaggatg gacaatagca taagcatctg gcagggtttc aggtatggaa tgatggtctg
    20281 tcacaatgac atctactccc atagactgag ccaatgcaat agcctcatga ccagcaaccc
    20341 cattgtccac cgtcacaatc aaggaaattc cttcttgctc gataaagtat ttataaacac
    20401 tagcattagg gccatagcca tcggtaaaac gatttggcag gtaaactcgg cactccgcac
    20461 caagttgttc caaactttcc ttcacaatag aagccgaagt catgccatcc gcatcatagt
    20521 ctccataaac gagaatattt tccccttctt caatagcctg acgaatccgc tccactgcct
    20581 tgtccatatc atggagcaga taagcatcat gtaagtcctc caaggaaggt tctaaaaact
    20641 tcttcagact ttcttggtcc tgaatccctc tctcaaacaa taaccgagcc acctcaggac
    20701 ccagtccagc cttcttggct atctttgtaa aatccgcatt ttctacctgc agggcaaact
    20761 gccattcata agtaggtgtt atcaaaaaga catccactct ttctaagaat tctattgctt
    20821 cattataaca tgatttagac gttttctcta tccagattgc ttttttataa cattttatag
    20881 tatattgaaa ctagaatagt acacctctac ttctaaaata ttgttagaaa tcgatttgat
    20941 tgtcccgatc gatttttcct gttcttatat cactatagtg aattttttca gatatgattt
    21001 gacaagaaca ttcttttatc gtagaatcat gttataaaat ctaacacaag ggagatctct
    21061 catggcttta gtagaattta aaaacgtcga aaaatattac ggagactacc acgcactccg
    21121 caacatcaat ctccgttttg aaaaaaggac aagttgttgt cctgcttgga ccttctggct
    21181 ctgggaagtc cactcttatc cgtacgatca atggtttaga ggctgttaac aaaggaagtc
    21241 tcctagtcaa tgggcaccaa gttgctggtg ccagccagaa agatttggta cctcttcgca
    21301 aggaagtcgg catggttttt caacatttta acctttatcc acacaaaaca gtgttagaaa
    21361 acgtaacact tgcacccatt gaagttctag gaattgataa aaaagaagct gaaaaaaccg
    21421 cccaaaaata tctggaattt gtaaatatgt gggacaagaa agattcctat cccgccatgc
    21481 tatctggtgg acaaaaacag cggatcgcca tcgctcgtgg tcttgctatg catccggaac
    21541 tcctcctctt tgatgaacca acatctgctc ttgatcctga gactatcgga gatgttctag
    21601 cagttatgca gaaactggcg catgatggga tgaacatgat catcgttacc cacgaaatgg
    21661 gctttgctcg agaggttgcg gaccgcatta tctttatggc cgacggagaa gttttagtag
    21721 atatgacaga tgtcgatagc ttttttgaca atccaagcga acctcgtgcc caacaattcc
    21781 tcagcaaaat tatcaaccac gaaagtgaca aagtcaaata aggaggcgcc tatgaaaaaa
    21841 aattcttttt atcagcatta ttgattagcc ttttcggcct tgttgctgcc aaaccagtcc
    21901 aggctaatac tagtatcgca gacattcaaa aaagaggcga actggttgtc ggcgtcaaac
    21961 aagacgttcc caattttggt tacaaagatc ccaagaccgg tacttattct ggtatcgaaa
    22021 ccgacttggc caagatggta gctgatgaac tcaaggtcaa gattcgctat gtgccggtta
    22081 cagcacaaac ccgcggcccc cttctagaca atgaacaggt cgatatggat atcgcgacct
    22141 ttaccatcac ggacgaacgc aaaaaactct acaactttac cagtccctac tacacagacg
    22201 cttctggatt tttggtcaat aaatctgcca aaatcaaaaa gattgaggac ctaaacggca
    22261 aaaccatcgg agtcgcccaa ggctccatca cccaacgcct gattactgaa ctgggcaaaa
    22321 agaaaggtct aacctttaaa tttgtcgaac ttggttccta cccagaattg attacttccc
    22381 ttcacgctca tcgtattgat gccttttccg ttgaccgctc tattctgtct ggctacacta
    22441 gtaaacgcac agaactacta gatgatagtt tcaagccatc tgactacggt attgttacca
    22501 agaaatcaaa tacagagctc aacgactatc ttgataactt ggttactaaa tggagcaagg
    22561 atggtagttt gcagaaactt tatgaccgtt acaagctcaa accatctagc catactgcag
    22621 attaaggagg acaccccatg acagatttat catcttggac agcctatttt caggattttg
    22681 gacaattttt caatggtttc ctctttactc ttgccctagc gattggatcc tttatcctcg
    22741 ccatggtctt aggaatcttc tttggtgcca tgtcaaccag taaacgtcca attttacgca
    22801 ttttagctcg catctttgtt gaattttacc aaaatactcc cctcttggtg cagtttgtta
    22861 ttgtttttta tggcctacct cttatcagtg accatatcat catgattcca atttattgga
    22921 cagccgttct ctgcgtggga ctctatcacg gcgcttatat cgctgaggtt attcgttcag
    22981 ggattcagtc tattcctagt ggtcagatgg aggccgcctt gtcgcaaggt tttacctata
    23041 tcagtgccat gcgcttgatt accttgcctc aggccttctg catcattcta cctccattga
    23101 ccaaccaaat tgttaacctc atcaagaaca cctctactgt agctatcatc tctggagtag
    23161 acttgatgtt tgtgactaag tcttggtcgg ctctcaacgg aaactatatc ccagcctttt
    23221 taggtgctgc tcttctctac tttgccctat gcttccctgt tgcccagttt ggtcgcaaga
    23281 tggagcaagc caacaaaaaa gcctattcac tttaggaggt tactatggaa tctattttag
    23341 aagttttaac cccagataac ctagtcttta tctttaaagg atttggcttg accctctata
    23401 tttctctgat tgccatcatc ctctctacta tcatcggtac ggtgctagct gtcatgagaa
    23461 atggcaaaaa tcctatctta cgcattattt ccagtattta tatcgagttt gtgcgcaacg
    23521 ttcccaacct tctctggatt tttactatct ttttggtgtt caaaataaag tccacaccag
    23581 caggtattac agcctttact ctctttacat cagcagcctt ggctgagatt attcgaggcg
    23641 gtctcaatgc cgtagacaag ggacaatacg aagcaggaat gtcacaaggc ttcacctcag
    23701 cccaaatcct ctactacatc attctcccac aagccatccg caaaatgcta ccagccatca
    23761 tttctcagtt tgttcccgtg attaaggata ccagtctcct ctactctgtt atcgccctac
    23821 aagaactctt tggagccagc caaattctca tgggccgtta tttcgaacca gagcaggtct
    23881 tcagtcttta catcctgatt gccctcatct acttcagctt taacctagca atttctagcc
    23941 tgtctcatat gctagccaaa cgttggcaac aagctgcaga ataatactct tcgaaaatct
    24001 cttcaaacca cgtctgcttc accttgccgt atatatgtta ctgacttcgt cagttctatc
    24061 cacaacctca aaacggtgtt ttgagcagcc tgcggctagc ttcctagttt gctctttgat
    24121 tttcattgag tataaacagc cactctccag ttcaattgga gagttttttt gatgagagta
    24181 gatatttata gacctcaatt tgacaccata cattttctat gataaaatgt aacaaattta
    24241 tttacaacaa atcgaaatct aaatgaaatg gaattttcta tgaaatcaat caaaggacta
    24301 ctctttatca tagctagttt tatcttgact cttttgactt ggatgaacac ttctccccaa
    24361 ttcatgattc caggactagc tttaacaagc ctatctctga cttttatcct agccactcgt
    24421 ctcccactac tagaaagctg gtttcacggt ttggagaagg tctacaccgt ccacaaattc
    24481 acagcctttc tctccatcat cctactaatc tttcataact ttagtatggg cggtttgtgg
    24541 ggctctcgct tagctgctca gtttggcaat cttgccatct atatctttgc cagcatcatc
    24601 cttgtcgcct atttaggcaa atacatccaa tacgaagctt ggcgatggat tcaccgcctg
    24661 gtttacctag cctatatttt aggactcttt cacatctaca tgataatggg caatcgtctc
    24721 cttacattta atcttctaag ttttcttgtt ggtagctatg cccttttagg cttactagct
    24781 ggtttttata tcatttttct atatcaaaag atttccttcc cctatctagg gaaaattacc
    24841 catctcaaac gcttaaatca cgatactaga gaaattcaaa tccatcttag cagacctttc
    24901 aactatcaat caggacaatt tgcctttcta aagattttcc aagaaggctt tgaaagtgct
    24961 ccgcatccct tttctatctc aggaggtcat ggtcaaactc tttactttac tgttaaaaat
    25021 tcaggcgacc ataccaagaa tatctatgat aatcttcaag ccggcagcaa agtaacccta
    25081 gacagagctt acggacacat gatcatagaa gaaggacgag aaaatcaggt ttggattgct
    25141 ggaggtattg ggatcacccc cttcatctct tacatccgtg aacatcctat tttagataaa
    25201 cgggttcact tctactatag cttccgtgga gaagaaaatg cagtctacct tgatttactc
    25261 cgtgactatg ctcagaaaaa tcccaatttt gaactccatc taatcgacag tacgaaagac
    25321 ggctatctta attttgaaca aaaagaagtg cccgaacatg caaccgtcta tatgtgtagt
    25381 cctatttcta tgatgaaggc acttgccaaa cagattaaga aacaaaatcc aaaaacagag
    25441 cttatttacg aaggattcaa gttcaaataa gtaatcatac tctcctggga aatcccagga
    25501 gagtttttta tagccaaatg aaataagaca ggaacgatgg aattttggca ttcaaatcaa
    25561 ttgataagac tattttatag tggagtgaaa taggatacga acaaattgat taggaaaatc
    25621 aaatgaattt atagaaatct tttagcagtt atgttatcct attctagttt caaaacgcta
    25681 tagaagcagc attgtactag tctagattca gtttactata ctaaaacgag tagcttgaaa
    25741 tcaaaaaacc caccctcaca ggcaggtttt atctgtatta ttcagctaga ttatgcttta
    25801 ccttctgaac cgaatacgtc gatacgttct tcaaccgatg cttggatagc ttttacaccg
    25861 tcagccaaga atttacgtgg gtcgaagagt tttttcttgt cgtattctgc ttcgtttgct
    25921 tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg cgatttggca ttctgtgtta
    25981 acgttaactt tggcaacacc aagtttgata gctgcttgga tttgctcatc aggaatacct
    26041 gatccaccgt gcaatacgat tgggaatcct ggaagagctt ctgtcaattt ttgcaagtgg
    26101 tcaaggtcaa gaccttccca gtttactggg taaggaccgt ggatgttacc gataccagct
    26161 gccaagaagt cgataccagt ttcaaccatt gctttagcgt cttcgattgg agccaattca
    26221 cctttaccga tgattccgtc ttcttcacca ccgatagtac caacttcagc ttctactgag
    26281 atacctttag cgtgtgcttt ttcaacaact tctttagcca atttaaggtt ttcttcaact
    26341 ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa cttcgataca ctcaagtgca
    26401 tcttcgtagt gaccgtggtc aaggtggata gctactggta cagtgatacc cattgattca
    26461 acaaggttag cgatcaagtt gcgagcaact ttgtaaccac ccatgtattt agcagcaccc
    26521 attgaagttt ggatcaaaac tggagctttt ttagcttctg ctgcgcgcaa gatagcttga
    26581 gtccactcaa ggttgtttgt gttaaatcca ccaactgcat aaccgttgtc acgggctgct
    26641 tggacaaatt tttctgctga aacgattgcc attttatcag gcctcctgta tatttttatg
    26701 ggtcatccca tttacattgt tcattttatc actttttgcc aaaaaaatct agtttttccc
    26761 gcagtttcga ttgattttct tctaactcca tctatgtaaa ccctttctct ccctagtctt
    26821 gggcgacttt tggaaaagct ataaagaagg ttaaacgatt ctcctccatc tcgaaacgat
    26881 aagctaattt ttcatgttct aatagactct taaccacaaa gagccccata ccagacccct
    26941 tgaccttgcg actggcattg tcagaaaaag actgggctag tttttcttgt tcctctgagc
    27001 tacagctatt ttcgataaaa agttctcctt ctctttctcc aattcgaact aagccacctg
    27061 gaacagagtg cttaatggca ttgctgatga gattagaaag aatcaacttc ataactgatg
    27121 ggtttagata agcctgctga tgggtcaaac tattgtctat ctggagctct ctttccttgg
    27181 ctagcaaggc ataatctttg accagatttt gcgtcatctg gaggaggtca attgtttccc
    27241 tatcatctcg caattcctgc acagaagaga gggaaagtat ctgcagaaca tggtgattga
    27301 gttcatccac aatccccaag gcaactccca gatactggtc tctatcctta taacgaccga
    27361 tattctctct catattttcg attaggattt tcaaactagc cagcggtgtt ttcaattcat
    27421 gagaagctcc tcgtaggaat tcgaccttca tcttctccag ctggagaatg gcttcattct
    27481 tttcatgcaa gtccgcaata acagtcaaga gatgctggta gaggctattg atttgttcct
    27541 tgagattacc tatctcatcc ttagaatcca cgcgcaatcg cacttgggaa tccaggtcca
    27601 tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc aacaatagtc cgggcgtaga
    27661 tgtaggccac caagagggaa atcagaaagg aggccagcaa ggtataggga agaaactgga
    27721 gactgatttg ctccgcttcc ttttgtaaat ccatggaagc tagaaactgg agaatcatag
    27781 taccaccgtc ttgcgttttc acctcgcgct cctcaataaa gagagaggtt gtctggcggt
    27841 ctgtgtccag aggaagactg tccttgactt ctaacttgtc ctcggtcatc tcacctttga
    27901 cggtcccctt gatatcacta gtctgggaat acaagtctaa cacttgctcg atactctgcc
    27961 tatctttccc ttctagggac tgggcaatgg ctgttgcctt ttgaccaatg gtttcctgac
    28021 gatgactcag ataagtcgaa ggaaaaagaa aataaatagc taaatgaagg cagataacca
    28081 gaacactaaa tatcgagaag gtatagataa atatctttgc aaataaacct gttcgtttca
    28141 ttttcgctcc aatttataac caacattgcg cacagtgagg atacaatcca agtctagctt
    28201 tttccgcaat tccttgatat aaacatcaat aacacggtca aagggaacct catctgtcgc
    28261 tttccagacg gcatcgataa tctgagaccg agtcaaggcc cggccttcat ttttcactag
    28321 atagtccaga atttccaact ctttggcatt gataggcact tcttgacctg cgaggcttgc
    28381 actgtagctt tcaaagtcca ccttggtatc cttgtaagaa aagattcgtc ctgtatcgta
    28441 gtagcgcttg aaaatcgcgt ccaccctcac ttttaagagg gagagagaga aaggtttttc
    28501 cagatagcca tctgccaaag aggcaaaggc actcatcttg tattcctcat cctgaaaagc
    28561 tgtcaacatc aagacaggaa cctgactggt tttacgaatc tcagctagga cttctaagcc
    28621 gttaagcttg ggcatctgga tatccagtaa aaccagggcc acctcatagc tagaaaattg
    28681 ctccagagct tcctgaccgt ccgctgcctc aatagtttca tagccacaat ccgtcaaata
    28741 atcactgacc ccctcacgga tcatctcttc atcttctaca attaaaattt tcatacttta
    28801 actgctctct attttttatt tttcttagaa taaataccta ctctattttc tattatagtc
    28861 tcttgctggc cttttgtctg taagcaactg atcactagat aaaacgttgt gaaattcctt
    28921 tctcataaat tccataactt tagtatatta tatttaagca ctaaagtaca aagaaagcaa
    28981 ctgaaagcaa tgattttcac cactgctttc agatttattt tgaattgtta aatagctatt
    29041 cctatccact attcttgaat agaaacacaa gatgcaatct ttattccaga ctcatttttt
    29101 caaatttatt caccatccag caagagctct tttggttgtt ttctaaggag attgcttgaa
    29161 gcaagcgcca taacgagaac cactagaacc aaggcaagga caaaaatgat gataaagtct
    29221 gatgtctgaa tggaaatgtc taggctcgac aaggtcttgc taaagccatc tacttctgca
    29281 ccgccaccaa ggttagaggc ttgagccgcc ttactagcct gtttggcaac acctgaagtc
    29341 acattggcaa ggacagtgtt tccaattgca cgggcagtgt aattagctag gaagtaagca
    29401 gaaactagag cagggatagc aatcaagata gattcggtga tgaattgacc caagatactt
    29461 gcctgcttga ggccgataga gaggagaatt cccacttcct tgcgacgggc gttgatccaa
    29521 aggctgagca agagggcaag gaggagaact gagaagctca agctacccca gaagaggagg
    29581 ttggccatct tgtacatacc agagatagat tgctcaagag ctgggtagtt agaggagctc
    29641 ttgacgagtg tgtagctctt ccagttgata ccactgatgc cattcaactc tttcataaca
    29701 tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt ccccataaat ggctgtgtct
    29761 tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg taatagctgt gttttcgtaa
    29821 agttcttgtg agtaggttac tgctgactta ttatgaccat caaagagtcc cttgattgtc
    29881 acttcaactg tttccttggc tcctttttca ttatctgcat cgtagatatt agagtccagt
    29941 ttaaccttgt cccctacttt ccagccgtgt ttggctgcca agtccttgtg caagaggatt
    30001 ttatccttgt cgtcgttggt taagtgctct ccttcgacta gtttataaga accagagaca
    30061 aacttatctt ctttagagga gtcattgaca cctgtaatca tcaagctact tccaaaacgc
    30121 ttggcacgat cagcagtgag attcttcttg gtttctggcg tttcaatcag gtcatatcca
    30181 gtcaaatctc cgatagcgtt gatacgtttg acataagact cgatggcctt gttttcggtg
    30241 atttttttga tgtcttcacc cttgatattc ccagcaccac gaggcgttcc ttggttgacg
    30301 cgacgattga tttgcatgga gaagctattg gtgatatttt taaaggtctc ctgagaagcc
    30361 ttggcagtag ctcccttgat tgacaagccg accaaactca agctcgccat gaggagaata
    30421 atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa cataggcaaa tgcgttgtgt
    30481 aacatagatt ccctttctag attttgtttt aatcattcta tcaaaataag ctcaaattat
    30541 ttactagtat tgcgcgtttc agtcagtttc ttatccttta attcaagtgt aatatctgac
    30601 gcttgtgcca cttctttact gtgagttacg acaatcacac atttacctgt tttctgggca
    30661 agtgatttga gtagttcgac aatatctcca gcagttttag gatccagatt tcctgttggc
    30721 tcatcagcta gaataactgg agcttctgag accaaactgc gagcaatggc aacacgttgc
    30781 tgttgaccac ctgataactg gagaacattc cgcttgatct ggctttcatc caaaccaagc
    30841 tcaagaagtg tattcttgct tgcctttttg ttgaccaatc ggatattttc cagcggagaa
    30901 agataatcta tcaagttata attttgaaag accagggaaa tatggtgcat gcgatggtaa
    30961 gaatagccct tcttacgaat atcctctcct tgaaaaagga tagaaccttc aacaggacta
    31021 tctagaccag caagtaggga caagagtgtg gattttcctg ctcctgactc cccaataata
    31081 ctgtaaaatt ttccgggttc aaaattataa tcgatctgat ataggactgc ttcagcagta
    31141 ttcttataac ggtaggtaac atcttgtaat tgtaataaag tcatgatttc tccttcttaa
    31201 ctaatagatg ataaaatttc tttcggtgat tttctaaata agaataggaa acaaagggct
    31261 acagataagc aactaagcag aactagaaaa acataggatt ctgcaaaaga taagatacta
    31321 gttgataaac tgcttgcttt ggctagtgta tcttgtaagc ttgcctgatc tccacttgct
    31381 agtagagttt ggagtaggta agttgtgatt ccgtttcctg caacaaatgc tggaagcaaa
    31441 gctccaagag ataccaaaac tacctctaaa cagaattgta ggaagatcga gctcttgcct
    31501 tttccaagtg caagtaaaat ccccacttca tagacccgtt ctctcaacca gagagacaaa
    31561 accagaatta aggctccagc tcctgctatc aacatcccat aaaggaagat ggtcaggaag
    31621 gtttggaaag ttgcaactga gtctttgatt tgttcaaaag ccttgttttc cttttcgact
    31681 tggtagcctt gattttccaa ggccaagttt tctacctgct tcatgagtcc gtccatttcc
    31741 ttaggatttt ctacatagaa gcgtgctgca ctgacttgag cttcactatt gcccaaaagg
    31801 gtttggctac tttcatagtc tgtaaagact tgattttcac tgaagtcaga agacaagcct
    31861 gtgaatttct cttgtttttt accagaaaag atgccgataa tctcaaactc tactgtttgt
    31921 ccttttccag attcagactg accagcatcc aagccaatct tgtcatgaag tgaaagaccg
    31981 ttcttcttag ccaattcttc gtggataagg attttcttgg aatccccttt ttgaaggtgt
    32041 cgcccttctt ttagattgaa agccgaactg gtaaaggtta catccttgga tgaatcctca
    32101 agagccgtta agctaaccaa gttattgtct gcagctgata aatcatcacg ctccacgctc
    32161 tgctcgccag tcactgcttc cttgtctttt agttttgcga ccgtctcaag ttcaggagag
    32221 acattttcca gccccttaat cttgcttaca gatgctaggt ctgacaactt gaatgtctga
    32281 ccattctcta tcttcttaat agaaaaagat gtattgagtg atttataaag attgctttct
    32341 actgttttgt tggacttcat cagagtcaaa caggctgaaa ttccggccaa taagaccaat
    32401 aaaatcagaa ataaaataaa acttctcagt cgctttctgc tgacataagc ccaagatctt
    32461 tggattggat tcatttgtca cctccatatt tgtaagacta ttataaaacc caaatatgaa
    32521 atgtttatga aatacgaaaa aaaaa
//
LOCUS       DAVMMR010000021        32513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMMR010000021 DAVMMR010000000
VERSION     DAVMMR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.021"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1157)
                     /gene="guaA"
                     /locus_tag="VYS74_000942"
     CDS             complement(<1..1157)
                     /gene="guaA"
                     /locus_tag="VYS74_000942"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV3789000.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     ICETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTEL"
     gene            1299..1997
                     /locus_tag="VYS74_000943"
     CDS             1299..1997
                     /locus_tag="VYS74_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3789001.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2040..2936)
                     /locus_tag="VYS74_000944"
     CDS             complement(2040..2936)
                     /locus_tag="VYS74_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3789002.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGILQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(2945..3880)
                     /locus_tag="VYS74_000945"
     CDS             complement(2945..3880)
                     /locus_tag="VYS74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV3789003.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(3877..4602)
                     /locus_tag="VYS74_000946"
     CDS             complement(3877..4602)
                     /locus_tag="VYS74_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV3789004.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESIEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(4686..5321)
                     /locus_tag="VYS74_000947"
     CDS             complement(4686..5321)
                     /locus_tag="VYS74_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV3789005.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5323..6150)
                     /locus_tag="VYS74_000948"
     CDS             complement(5323..6150)
                     /locus_tag="VYS74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3789006.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6172..6507)
                     /locus_tag="VYS74_000949"
     CDS             complement(6172..6507)
                     /locus_tag="VYS74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV3789007.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(6508..7224)
                     /locus_tag="VYS74_000950"
     CDS             complement(6508..7224)
                     /locus_tag="VYS74_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV3789008.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            7737..8348
                     /locus_tag="VYS74_000951"
     CDS             7737..8348
                     /locus_tag="VYS74_000951"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3789009.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            8393..9121
                     /gene="rlmB"
                     /locus_tag="VYS74_000952"
     CDS             8393..9121
                     /gene="rlmB"
                     /locus_tag="VYS74_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV3789010.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9160..10071)
                     /gene="trxB"
                     /locus_tag="VYS74_000953"
     CDS             complement(9160..10071)
                     /gene="trxB"
                     /locus_tag="VYS74_000953"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV3789011.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTNNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10137..10361)
                     /locus_tag="VYS74_000954"
     CDS             complement(10137..10361)
                     /locus_tag="VYS74_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV3789012.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(10439..11182)
                     /locus_tag="VYS74_000955"
     CDS             complement(10439..11182)
                     /locus_tag="VYS74_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3789013.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11182..11982)
                     /locus_tag="VYS74_000956"
     CDS             complement(11182..11982)
                     /locus_tag="VYS74_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3789014.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12140..12496)
                     /locus_tag="VYS74_000957"
     CDS             complement(12140..12496)
                     /locus_tag="VYS74_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV3789015.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(12509..13021)
                     /locus_tag="VYS74_000958"
     CDS             complement(12509..13021)
                     /locus_tag="VYS74_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV3789016.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13021..13515)
                     /locus_tag="VYS74_000959"
     CDS             complement(13021..13515)
                     /locus_tag="VYS74_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789017.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            13650..14096
                     /locus_tag="VYS74_000960"
     CDS             13650..14096
                     /locus_tag="VYS74_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV3789018.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14093..14740
                     /locus_tag="VYS74_000961"
     CDS             14093..14740
                     /locus_tag="VYS74_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV3789019.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(14761..15342)
                     /gene="pdxT"
                     /locus_tag="VYS74_000962"
     CDS             complement(14761..15342)
                     /gene="pdxT"
                     /locus_tag="VYS74_000962"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV3789020.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15343..16218)
                     /gene="pdxS"
                     /locus_tag="VYS74_000963"
     CDS             complement(15343..16218)
                     /gene="pdxS"
                     /locus_tag="VYS74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV3789021.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16370..17749)
                     /locus_tag="VYS74_000964"
     CDS             complement(16370..17749)
                     /locus_tag="VYS74_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV3789022.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18043..18966
                     /locus_tag="VYS74_000965"
     CDS             18043..18966
                     /locus_tag="VYS74_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV3789023.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19026..19631
                     /locus_tag="VYS74_000966"
     CDS             19026..19631
                     /locus_tag="VYS74_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3789024.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            19650..20894
                     /locus_tag="VYS74_000967"
     CDS             19650..20894
                     /locus_tag="VYS74_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3789025.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21117..21374)
                     /locus_tag="VYS74_000968"
     CDS             complement(21117..21374)
                     /locus_tag="VYS74_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV3789026.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(21416..23452)
                     /locus_tag="VYS74_000969"
     CDS             complement(21416..23452)
                     /locus_tag="VYS74_000969"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV3789027.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(23664..24581)
                     /gene="glyQ"
                     /locus_tag="VYS74_000970"
     CDS             complement(23664..24581)
                     /gene="glyQ"
                     /locus_tag="VYS74_000970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV3789028.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(24774..25535)
                     /locus_tag="VYS74_000971"
     CDS             complement(24774..25535)
                     /locus_tag="VYS74_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV3789029.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(25808..26650)
                     /locus_tag="VYS74_000972"
     CDS             complement(25808..26650)
                     /locus_tag="VYS74_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV3789030.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(26764..28155)
                     /locus_tag="VYS74_000973"
     CDS             complement(26764..28155)
                     /locus_tag="VYS74_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV3789031.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTPFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(28393..28857)
                     /locus_tag="VYS74_000974"
                     /pseudo
     CDS             complement(28393..28857)
                     /locus_tag="VYS74_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29011..29988
                     /locus_tag="VYS74_000975"
     CDS             29011..29988
                     /locus_tag="VYS74_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3789032.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            29985..31067
                     /locus_tag="VYS74_000976"
     CDS             29985..31067
                     /locus_tag="VYS74_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3789033.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31131..32161)
                     /locus_tag="VYS74_000977"
                     /pseudo
     CDS             complement(31131..32161)
                     /locus_tag="VYS74_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9619 a         7261 c         5862 g         9771 t
ORIGIN      
        1 agctctgtac caagagcacg aacttcatcc ttgtaaagag tattgagtgg ttcaatcaat
       61 tcaaactgca tatcttctgg aagaccaccc acgttgtggt gtgacttgat agtttgagct
      121 gtatccgtac cagactcgat aacatctgta tataaagtac cttgagcaag gaatttcaca
      181 tctttgagct tgcttgcttc gtcatcgaat acatagacaa actcgttacc aatgatttta
      241 cgtttttgtt caggatcaga aacgccagca agtttgtcaa ggaaacgttt agcagcgtct
      301 gctttgacga tattcaaacc aaacttacca ccgagcatgt ccataacttg atcagcttcg
      361 cctttacgaa gaagaccgtg gtctacgaag atacagatca attgatcgcc aatcgctttt
      421 tggagaagaa ccccaacgac agatgagtca acaccacctg atagaccaag aaggacacgt
      481 ttatcaccga cggtttcaca aatttttttg atctgcatgt caatgaaatt atccattgac
      541 cagtcacctt tagccttaca aatgttaagg gcaaagttac gaaggatatc atttccgtat
      601 acagaatgac gaacttctgg gtggaattgg ataccgtaaa tgtgtttatc tgggttttcg
      661 atggctgcgt atgggcagtc agctgatgta cctgtacgaa caaagtcagc aggaatctca
      721 gtaaccgcat caccatggct catcaaaaca gtctgttcat caggtgttga ttcaaaaagc
      781 gctgatggtg tgtgagttag ggttgattga ccgtattcac gatttccagc atcacctgca
      841 ggaacaactt ttcctccaag tttatgggtc aataactgca taccataaca gattcccaaa
      901 attggaattc cgagttcgaa gatttctggg tcaatatcaa atgaaccatc ttcatataca
      961 gaatttggac cacctgatag aataattcct acaggattga cttcacgaac ttcagcagct
     1021 gaaattttat ggctttttag ttctgaaaaa acaccaatct cacggatacg gcgtgaaatc
     1081 agctggttgt actggctacc atagtccaat acgatgattt tttctacatc ttgcaaatca
     1141 gttgaaatgt tgctcatctt tttcctttct caaaagaaat atcttttccc aatattctat
     1201 cacaaataag ggcgaattac caactaaaca aggcgaagtt tgactttttc tagtttattg
     1261 gggtacaata gagaaaagat agaaatgaag tgaaaaatat gctacctgct tatataaaaa
     1321 tccatgatca gataaaaaag gatattgacg agtaccgttg ggctattggt gagaggcttc
     1381 ccagtgaaag agatttagct gagcagtttg cggtcagtcg catgaccctc cgccaagccg
     1441 tatctctatt agtcgaagaa ggcgtcttag agcgccgtgt aggaagcggc acctttgttt
     1501 ccagtactcg agtacaagaa aagatgcgag ggacaaccag ttttactgaa attgtcaaat
     1561 cccaaggtaa agttccctct agccagctca tttcctacaa aaaaaccatt cccaatgagc
     1621 aagaagttgc caagctagga atttttccaa cggacaatat tatccgaatg gagcgggtcc
     1681 gctatgccga ccaagttccc ctagtttatg aagttgcttc tattcctgaa aaattcatta
     1741 aggactttaa aaaagaagaa atcaccagtc atttcttcca aaccttgcaa aaacatggct
     1801 atcgtatcgg caaatctcaa cagaccatct atgctcgcct tgctaaagaa aagattgccc
     1861 actatttgga agttgaaaaa ggacatgcta ttcttggatt gacccaggtt tcctacctag
     1921 aagatggtac tgcttttgaa tacgtaaaaa gtcaatatgt aggcgaacgc tttgaatttt
     1981 atcttgaaaa taattagatt accacgcaaa aactctctgt cacggacaaa gagtcttttt
     2041 tatttttcta agaataaatc tttcaaagaa atcaaagtta cagcaaacaa tatcattgcc
     2101 ataccaataa aatcaatggg ataaaattgt tcattcatta ttaagaaggc aaagaaaacc
     2161 gccgatattg gctcaattga agccaacaag cttgacttga ccggtcctat cagactggct
     2221 cctttaagga aagctgtata ggcaaagaca gtcccgataa ggataatgcc tgcaaacgca
     2281 aggagaaaat caagactagt cgggatatcg gcctgtagaa tccctgtaaa aggaagggcg
     2341 accaaacctg ctatgaccat tcccacacca atgaccaagc tgctccccca ctttttaatc
     2401 aaggctatgg gtaaaatgat atacaaagca taagtcaagg cagaaaagag accccagaac
     2461 agaccagcag gtgtcatgga taactggtcc aactgcccat gtgttgcgat caggaaggtt
     2521 cctccgatgg cgaatatgat ggaaactatc tctcccagtg tcggtgccac cctatccttg
     2581 atacagctat aaattaaaat tccgacagga caaacatact gaagcaccgt cgctgttccc
     2641 gcattggtct cctgaatagc agacagatag gcgaattggt tgagaaaaag accaatcaga
     2701 gcaaaaataa gaagagacag caaactcttt ctatccttta aaaagaccag tattttatcc
     2761 tttgcagtag cataagccaa gagcatgaga attccaccag cgattaaaag acgcaagttg
     2821 gtcaagacca gagccgaaat tccgtgtgcc attaagtatt ggccactcgt tcctgacaac
     2881 ccccaagcaa taccagccac aactgttagt aaagtccctt ttaaactatt tgacatgtct
     2941 ctccttactc ttctttgcga atcaagttca tcacttgatt gcggtctaat atccattgag
     3001 ctctctcgtc cttttcatag gtacgattcg ataggaaaat agtggcttct tgcttctgac
     3061 gattccacat gataaaggta cctgtatagc ccgtatggtc tagccaatct ccttccaaat
     3121 tccatgctaa agaacgttcc ttgtcatcca aaggagaaaa attttgattt aagtctcttg
     3181 caaaatcatc tgctaaatag tgttctaaaa agatttgtaa atcctttata gtcgaaaata
     3241 aaccagcact cccagcatgt ctacccagga gacgagcctt gggatcatgc actatgcctg
     3301 cctctacacc tctaactgtt ggaacagcaa gctcaactgg cccaaacttg gtttccgtca
     3361 ttccccaagg tttccagact tgatccttta aaatcacatc caaatcttga ttaaaaattc
     3421 tttccaaaat aaagcccaac agcaaaaaat ggacatccga ataaagaaag gctggctgac
     3481 ttcgtctgtt gagatgaaac atcgcttcct ttaattcagg ggctgttaaa agatcacgat
     3541 taggaataaa aggatcaagg tctgttgcat gagtcaagag ctggcgaata gtgatgtctg
     3601 gataatcact ctcaggtaaa aaatctatta ccagtctatc aatatctaat tgacctattt
     3661 cccacaagaa ggtacaaact gtgccaaccc caacaacctt gctgacacta gctaggtcat
     3721 aaactagtcc tgcctcagtc tgcaagccat gctctgggtc actctggcct aaatagaact
     3781 ctgtccattg attgtcctta aaatacgcaa aagaggctcc gggataaatc cctgcctcga
     3841 tttgttcttc tattttttta ataatcttgg tccacttcat acttcttcaa accacaattc
     3901 aacattattt ctatctttac caaggaagaa tttttcagac ttaggaataa aatattcaat
     3961 agactcaaac ttctggcgaa gacttgctat gtctaattca ttgaccaaga acttgagcat
     4021 agataagtcc caggtaaccg tattgtccac agtcaaatcc tgcccctgag ctggaataaa
     4081 atcaagggat gccccaattt cagatgattc caagaaactt tcgatatcag ttgggagatg
     4141 taattccata gaaatctcaa atttacttaa agaaattgat gccaaatctg tttgaaattc
     4201 aggcttttct cctacttcta ctagacttgc tctgtcatct tccgcatgaa tcaaaatcaa
     4261 atcatcttct ggtgagaaaa tttcaaaagc gtagccattt tgacctttat ataatcgatg
     4321 aatcgaatct gttttagata agattccttc aatttctaag ggattttcca ccttgacaat
     4381 caatctagct agtttttttc ttccctctac cttacgagta cgcatactgg gagcttcttc
     4441 taaaaccagc ttttcaagac ccgtttggtc acctagtgac agaaaggccg attcttctaa
     4501 caaggccttc attccaaggg tttcaatata aaatgtttca tttaattttc tattattagc
     4561 ttttaaagta ggaataatcc gtacaatctg atttacattc ataaattcct ccgtcacttt
     4621 ttattttaaa ggattttaga attttttaca agatattatg ctcaaattta acattattta
     4681 gctatttagt aaagatagtc tttcaaggct tttgacaaag cctgataacc agcaatactg
     4741 aggtgcagtc catcagttgt atattctttt ttgagttggc ctgcttggtc tgtcaaacaa
     4801 tcaaatactg gcacaaattc cacctgcata taggcagatg caagctcttg ataggcttga
     4861 ttccagttct gaattttttc attcgagcgg atatagactg cctgctggta ctcctctctc
     4921 tcattgacag gcaaaatgga aagcaattta atctctgtca atggataatc gcgagcaacg
     4981 gattgaatga tagcttcgag attattgaga gcctcattca caggaacatc ctttccgata
     5041 tcatttgtcc caatcagaag aaaaatttta tctactgctc caccatatag atgagcatca
     5101 aggttctcta gtaacagtcc tgtctgatag ccacgaattc ctcgattgac aatcgtcttt
     5161 gaagtcccaa atagctcctg tagaggataa tactcgacaa tggaatcccc aataaaaaga
     5221 atgtttggtt ctacaacaga aatgtgattt aggtgacgat acttagtttg aattttttct
     5281 tgttccttta ggagccaatt ttctaataac tgtactgcca cttcattctc ctttttctaa
     5341 ccagttttcc aagacttgat aaactcctcc ttggctgttg gctggtgcta gagcagtcgc
     5401 cacagctttg gctttctcat cagcattttc catcgcatag gcaattccag ccatttcaag
     5461 catttcaaca tcattttcac tatcaccaaa agccatgatt tcttgggatt tcaagtccca
     5521 gcgcttgagt aattcctcca agccccatgc tttatgaatc ccagcttgga ggatatcaat
     5581 gcaaccatag ccactggata cagctcggac acggccatca aagagagcat tgatttcttc
     5641 caaaaccgaa ctcaaacgtt cctcaccaac aaccatgctc atcttgagca caccaccaaa
     5701 gaggtcagat gttaattcat ccacaaattg catccgttgg tagaattttt caatcatttc
     5761 tggagtcata aaactttcaa gatctgtaaa aatcgtacct tccttgacaa aatcaccctt
     5821 catccccgtt acaacaaact ggtcctgaca cgctcgaccc ttgaaatgag tcaaagcctt
     5881 gttgacaatg gcgtcatccc atgtctgagc ctgaatcaat tcattgtttt caaaaatacg
     5941 agcgccatta gcaacaacca gaaccactcg atccaccaag ggactcagta gttgtctcat
     6001 gcggtgaatt tcattgcccg tcgcaatgac aaaacgaatg cccctttgat ccaactgatc
     6061 taaaatcttt tccaatcgtg ggagatcaag ctgacctcta gcatccagca aggtcccatc
     6121 catatctgtc gcaattacct taatcatcat ttactttcct ctggattcaa gctaatacca
     6181 cttcaatccc acatgttcat tcatttcttt ataggctgta tcaattcgtt ccttagtcgc
     6241 ttcatctaac tggtcacgat gactgccatc ctctataaaa tcttccaaaa tataagtttg
     6301 gtaatgatac aagggatatc cttctggtga aaaggttccc tttggtgttc gattgaccca
     6361 agtaggatga gctttagctg ttccgatagt tgtttttcca tccttcttct tgatggtgac
     6421 atccatgaga acaccacgtt cagtccactt agcattctct tcatctccca tagattcaat
     6481 tcgttgattg gaaatgaagt tccccattta atagacaatg agcttcttat ctccatcttt
     6541 ttccaccgtt tcagatggtt caacaacgtg aggatgccct ccaaagataa tatccgctcc
     6601 ccaatcaatc atcttgtgat aaagagcttt ttgttcttca gttggttcca atcgatactc
     6661 aacacccatc tgaggcatga taatggtgat atctgcttcc ttctctgccc gttcaacttc
     6721 agcctttatc ttatcttcgt ttaaatctga aagataacga ttatagtctt cctgagaaat
     6781 atactgctca attccattga aaccatagga ataggctaac aatgcaacct tgatagcatt
     6841 cacttcctta atgaccagcg gagcctgagc acgtggttcg tgcgtataaa ctccgattgg
     6901 agtgattcca gctttctcaa taatatcggc cattgaaata actccctcaa tttgcgaatc
     6961 caaaatatga ttatgagcta aatccagcac atgataacct gcctccttaa tagcatccat
     7021 aacttcaaca ggagcattaa agagaagata acccgctaaa taatgatcct tattaatggt
     7081 tccttcaaaa tcaccaatag ctaaatctgc ttgcttgagc caaggagtca catactcaaa
     7141 attttcatga aagtcatagg taccgtcttc ttttttagct gaaaagaaaa gtccatcgtg
     7201 gtagagcaaa tccccgtggg ccataattct ggcagttttt tcctcttcct actcctgaga
     7261 cttttgctta gtcccctctt gagaaatagt atctcgtttt tgactagtca agggattcct
     7321 tggaagcttt caaacaacaa gaccaagccc attgataaac caactgctag caagagtatc
     7381 gccacaaatc ccttattgct ccacttgcga taactcctaa aaagtttacc aagcccttca
     7441 taaaacgaaa agctaaacca ccctgatttc gattttgtct tctttgtatc ttcgttctcc
     7501 ctactttctt atgcaagcct tttcttttta ttatatcaca gataagtatt tctttcacaa
     7561 ttgaattgaa cttcctatct attttctata aatcctaaat gccataatgc tttcaattct
     7621 tgtcattttg tgatatcatg tagaggaaat gaactaatcc acagtggctt attccaagta
     7681 taccacttgg gctttggcag tagctaactg cactaaacat aatataagga gagaaaatgt
     7741 ctgcaataga acgtattaca aaagctgctc acttaattga tatgaacgat attatccgtg
     7801 aagggaatcc tactctacgc acggttgctg aggaagtcac tttcccccta tctgaccagg
     7861 aaatcatcct aggcgaaaag atgatgcaat tccttaaaca ttcccaagat cctgtcatgg
     7921 ctgaaaaaat gggactccgc ggtggtgttg gactggctgc tccccagtta gatatctcaa
     7981 aacgcattat cgctgttttg gtacctaata ttgttgaaga aggcgaaact ccacaggaag
     8041 cctacgattt ggaagccatt atgtacaatc caaaaatcgt ctctcactct gttcaagatg
     8101 ctgctcttgg cgaaggagaa ggttgcctgt ctgttgaccg taacgtgcct ggctatgttg
     8161 ttcgccatgc ccgcgttact gttgactact ttgacaaaga tggagaaaaa caccgtatca
     8221 aactcaaagg ctacaactcc attgttgttc agcatgaaat tgaccacatt aacggtatca
     8281 tgttttacga tcgcatcaat gaaaaagacc catttgcagt taaagatggt ttactgattc
     8341 ttgaataaag aaaatcccgt tgcaagacgg ggttttgtgt tataatagag gcatgaaaac
     8401 aaatgatatt gtctatggtg tccacgctgt taccgaagcc ctccttgcaa atacaggaaa
     8461 caaactctac ctccaagaag atctccgagg taagaatgtt gagaaagtca aggaactagc
     8521 tacagaaaag aaggtgtcca tttcttggac atcaaaaaaa tctctctctg agatgactga
     8581 aggtgctgtt catcaaggtt ttgttctacg agtgtctgaa tttgcctata gcgagctaga
     8641 ttacatcctt gcaaaaacac gccaagaaga aaatccactt ctattgattc tagatggtct
     8701 aaccgatccc cataatctgg gttctatctt gcgaacagcc gatgcgacca atgtttcagg
     8761 tgtcatcatt cccaagcacc gtactgtcgg agtaactcct gtcgttgcca aaacagccac
     8821 aggtgctatt gaacacgttc caattgcccg agtgaccaac ctcagtcaaa ccttagataa
     8881 acttaaggat gaaggtttct ggacctttgg aacggatatg aacggtactc cttgccacaa
     8941 gtggaataca aaagggaaaa tcgccctcat cattggaaat gaaggaaaag gtatctctag
     9001 caacatcaaa aaacaggtcg atgaaatgat taccattccg atgaatggac atgttcaaag
     9061 ccttaatgcc agtgttgctg cggccattct catgtacgaa gttttccgaa atagactata
     9121 aaaaagtttc cagtcatctg attggaaact tttttatgat taactatgtt ctgtaatgaa
     9181 tttataggct tcttgaccag cgatagctcc atctccaacc gctgttgtta cttggcgaag
     9241 gtctttcaag cgaacatctc caactgcaaa gataccgtcg actgcagttt tcatgtggtt
     9301 atttgtcaca atccatcctg cctgatcttg gatattcaat tctttaacaa aatcgctaag
     9361 agggtccaaa ccaacataga taaagacacc accgaaggct tgttctgtca cttgacctgt
     9421 tttcacattt tcaaatacga ctgattctac tcggttttca cccttgattt cccttactac
     9481 agaatcccag ataaagctga ttttttcatt cgcaaaggcg cgatcttgta aaaccttttg
     9541 ggcacgaagt tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac gagtcaagaa
     9601 gagggcttct tcaacagctg aatctccacc accaactacc aataaatctt ggtcacggaa
     9661 gaaagcacca tcacacacag cacagtaaga aacaccacga ctgttcagtt cttcttctcc
     9721 aggcactccc aaaggacggt gtttagaacc agttgctacg ataactgtac gtgtttcata
     9781 tgtttggtca tcagtcatca ctttcttaaa atcaccatgg tcttcgacat tttcaacata
     9841 accataaatg tgctcaacac caagattttc aagtggttca aacatctttt cagccaattc
     9901 aggtccacta atattagcgt atcctgggta attttcgata tcagatgtat tattcatctg
     9961 accacctggc agaccacctt caatcaaagc tacttttaga ttgcttcgag cagcatacaa
    10021 ggccgcagtc atccctgcag gtccagcacc gataataata gtatcgtaca tatagattcc
    10081 ttctttcttg ttgtaactat ctttattcta actctttctt gtcaatcacg caaggattat
    10141 gccttgaaaa caagaattaa actcataacc gcaaaggata atactgggac aaccaagact
    10201 ccaatcataa tcatatcata gagatgggct tggcgagccc tggctgtctt atcaactccc
    10261 gacatggctc tcagaccaat ccaaatccct aaaaaaatca gaacaaggat ggtggtcaag
    10321 atcaaactct cgaaatataa agaaaatagt tgcagtagca tgatttctct catttctatc
    10381 ttttttaaag agtaaactca gctagtccaa ctaactgagt tttcctttat ctattatatc
    10441 aaatataagt ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct ttaggggtgt
    10501 ggataatctc atccggagtt ccagactcga tgattttccc cttatctaag aagagaattt
    10561 tatccgcaac ttgggctaca aaggacatgt catgactgac caaaatcatg gtctgacctg
    10621 acttagcagc atctgcaata gacttttcta cttcaccgac caattctggg tcaagggctg
    10681 aagttggttc gtctaagagc aaaacatctg gtttcatagc aagcgcacgc gctagggcaa
    10741 cccgttgctt ctgtccacct gataaatggc gaggataatg gttttcacgg tccgaaagcc
    10801 caaccttagc caactcttcc ttggcaatct tagtcgcttc ttggtcagat aatttcttga
    10861 caacaaccaa gccttctttc acattatcaa gtgctgttcg gcgttcaaat aaattaaact
    10921 gttggaaaac catagacaac ttacggcgta gggcaaggat ttcttcttga gtgattttag
    10981 aaaaatcaac tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt tcaagataat
    11041 tgagactgcg aagaaaggtt gattttccag ctcctgaaga accaatcaag gctacgactt
    11101 ctcctttttg aatatccaag ttcagatgat ccaagacagt ctgtcctgaa aaggatttgc
    11161 ttaaattcga aatcttaatc attaacgaag gtctcctttc acatctgttt gcactgtatc
    11221 aggtgcagaa atagccattt ttctctcgat gaaacgaccg aggctttcaa ttccgatatt
    11281 gactacccaa taaacaaggg caacggagat gaagcgttca aaatagcgat aatcagctcc
    11341 acctagaatc tgagcttggg caaagacttc cacaacaccc gcactaaaag ctagagatgt
    11401 tcccttggtc aaaccgatga gggaattaat caaggttgga gtagctacca ccgctgcatt
    11461 aggaataatc actcgtcgat aaacttgcgc tcgggtcata cccagactgc gtgccgcctc
    11521 aatctcacca ggattaactg agagaatggc tgcacgaatg gtttcactag cataagctgc
    11581 ctcattaaag gcaaaagcga caatcgcaaa agctgcagct ggaatcgcat tgatattgag
    11641 accagttccc cattgctgat tgagggcttt caaagccaaa gggattccgt agtaggtcaa
    11701 catgagttgc accaaaatcg gtgtcccttt taagaaacta acaaagaagg cctgcaaggg
    11761 atataaaatc ttgacacgat tgatcttcac aatggcaaaa agaagcgcca aaaccaagcc
    11821 aaaaagggca ccaccaattg tcaacataat tgttgttgga agttgttgga caattctagg
    11881 gattccatca aagaccgaac gtaggctaaa cagcttgcca tccggaatca attgcatcaa
    11941 gttttggtac caatctgatg ctaaaatcgt tgtaacattc ataaaaacat cctcttctga
    12001 taatgattca ttctaccatg cttctgccgt caatgaaatt atttgctacg gtcagataca
    12061 gactttgtct acctactagt ttatcatact ttgaaacagg gaagttatat attttatcta
    12121 tcaaagagat agataaaaac tatttcagtc ccagttcttc ataagatttt cgataaccga
    12181 tttgcttaac agttccattt tctactaaaa tgggccgttt taacaacata ccgtcacttg
    12241 ctagcaactc agccgcttct tggtttgaca aacttcctac cttatctttt agccctaatt
    12301 cacggtattt gataccactg gtgttgaaaa attgcttcaa ttcaaatcct gaggtttcta
    12361 gccaattcaa aatgacttct tggctaggtg tttcttccac gatatggacg gctttatagt
    12421 ccacacctaa ttgatttaat tcttgttttg cttttttaca agttgaacat ttggggtatt
    12481 cgataaattc taacatgtgc ttctccttct atttgtttat atctttaaaa tctacgcctt
    12541 catgctccaa gagccaagct ttcttttcca ctcctgcagc atagcctgtc agacgcttgc
    12601 ctgctcccaa cacacgatga caaggtacta ggatagacca aggattgcgt cccactgctc
    12661 caccaattgc ttgagcagaa gccacttgca ggtcttgagc aatttgtcca taggtcactg
    12721 tctgaccata aggaatgccc tgtaaatagt cccaaactct cttttcaaaa ttcgttccga
    12781 ttggcgccaa gagcaagttg gataaatcct gaggcttgcc tttaaagtaa tcatctaagc
    12841 aagcaataac tgggtctaaa ataggatgac taacaacttc ttctatcgtt tcatctccta
    12901 gtcccctctc aaaatgcttc tgctcctgaa cccaaattcc atacaaataa tggtcatcag
    12961 ctacaagtga tagaatacca attggtgagg agtagagtat ttttacatac ttcttttgca
    13021 ttatttcttt aatttttgat aggccaagcg ttcgccatct actggcacct taccaacttg
    13081 tttaaaacca agtttttcaa aaatatgttg cataaccttg ttttcagcat gcgtatctga
    13141 gcgaaaatca agataatcaa aaccttcaat caagccctct aagaaggttt gagcaactcc
    13201 ttttccctgc acatctgctg ccacagcaat acggtgaaag actagatact ctgactctcc
    13261 agcttgccag tttccctcat aaatagcttc ataggcctcc tctggactct tggtcacagc
    13321 agcataggct agcagttctc cctcttccaa ggctacgtag gcttgccctg agatgatatc
    13381 atcgataata acatctgcat ttggatagcc attttgccac tggtcactac cagcatctgc
    13441 taaacatttt ttggcatcct ccatcacctg catgatggca tctacttcat ttggaaaagc
    13501 taaacgaatt tccatctttt ttcctcattt ctgactagtt tctcccatca tagcataatt
    13561 taagtatttc tacaagcagt taaaacttga ctttgtgttt ttgttatttt ataatctagt
    13621 tatcaaaact atataaaaag gagttgaaaa tgaaaactac cttttcctac ccaaaatggg
    13681 cagaaattcc aaacattgac ctctatctgg accaggtttt gctctatgtc aatcaggtct
    13741 gcgcccctat ctctcctaat aaagacaagg gcctaacagc atctatggtc aacaattatg
    13801 tgaaaaatgg ttacctgaca aagcctgaca aaaaaaaata ccaacgccaa cagattgccc
    13861 gtttgattgc tatcacaacc ctcaagtctg tattttctat tcaagaaata gcccagacac
    13921 ttaatactct acaaactcaa gcaagttcag accaactcta cgatgctttt gtggactaca
    13981 tgaaccaagg aattgaccca gctaacccta ttatccaaac cagctgccaa accgtcaaac
    14041 tctatcatca aactctagac ttaatcgatc atactcaaga ggaggtaatc caatgaacac
    14101 tagtcttaaa ctcagcaaac aactcagttt tggagaggag attgctaata gcgtgaccca
    14161 tgctgtgggt gcagtcatca tgcttatctt gctgcctatt tcatccatct atagttatga
    14221 agcacacgga tttttatcct ctatcggcgt ttccattttc gtcatcagtc tctttctcat
    14281 gttcctatca tccaccattt atcactctat ggcctatggt tcgacccaca aatatgtttt
    14341 gcgaatcatt gaccattcta tgatttacgt tgccattgcc ggctcataca cgcccgttgt
    14401 cttgaccttg atgaataact ggtttggcta tctgattatt gtcatccaat ggggaacgac
    14461 catctttggt attctctata aaatctttgc taaaaaggtc aatgagaaat ttagccttgc
    14521 tctttacctg attatgggct ggttggttct ggctatcatt cctgccatta tcagtcaaac
    14581 gacacccgtt ttctggagtc tcatggtaac tggcggactc tgttatacag ttggagctgg
    14641 attttatgcc aagaaaaaac cttatttcca catgatttgg catctcttta tcctagctgc
    14701 gtccgcactt caatacatcg ctattgttta ttacatgtaa aaaagttgag aaattcaatc
    14761 tcaacttttt tctttacaca tattgataaa gtactggtgc aagcgcacat catcagtcaa
    14821 ttctggatga aaagaactta ccaacatatt tttttcttgg gctgcaacaa tttgattgtt
    14881 cactgttgct agaatttcta caccctcacc aacactactg ataatcggac cacggataaa
    14941 ggtcattgga atcttgccaa ctcccttaca ttctgcttcc gtgtagaaac ttcctaattg
    15001 gcgcccataa gcattacgct cgaccaccat atccatagtt cctagatgac tctctttctg
    15061 agaagtgatt tccttagcca gcaaaattaa gcccgcacag gtcccaaaca ctggtaagcc
    15121 agatagaatg gcttctcgga tgggaagtag catgttctgg tcacgtaaga gcttgcccat
    15181 ggttgtagac tcaccaccag gcaaaatcaa acccgacaag tcactctgat cttgctgaaa
    15241 atcatctaga tttctgagtt ctacactctc gacacctaat tgatctagca cttttgcatg
    15301 ttctgcaaag gccccttgca aggccaatat tccgattttc atctattttc ctcgttcagc
    15361 catgagaatt tggatttcat tttcattaat accaaccatg gcttctccta aatcttcaga
    15421 gatttgagct aggatttgag gattacggaa gttagtcaca gccttaacaa tggcactcgc
    15481 tcgtttaaca ggatctcctg acttgaaaat acctgaaccg acaaagaccc cctctgcccc
    15541 taattgcatc attaacgcag catctgctgg cgttgcaaca cctccagcag cgaaatttac
    15601 aactggcaat tttccatgtt catgaacata ttggaccaat tctacaggga cttgcaaatc
    15661 cttggcagca acataaagct cgtcctcacg taagttttga atgcggcgaa tttcctgatt
    15721 catcatacgc atatgacgaa cagcttggac gatatcccct gtccctggtt ctcctttggt
    15781 acgaatcatg gaagcacctt cagcgatacg acgcaaggct tcacccaaat ccttagcacc
    15841 acagacaaaa ggaacttgga attctttctt gtccacatgg aaacggtcat cagctggaga
    15901 tagaacttca ctctcgtcga tataatcaat ttcaatagcc tctaaaatct gagcttcaac
    15961 aaaatgcccg attctgacct tagccattac tggaatacta accgcttctt ggatttcctt
    16021 aatcatcttt gggtcgctca tgcgggaaac tcctccagct gcacgaatat cagccggaat
    16081 tcgttccaag gccatcacag ctgccgcacc agcagcttct gcgatacgag cctgttcagg
    16141 attctgcaca tccataataa caccaccctt gagcatctgt gccaagtttt tatttagttc
    16201 ataacgattt tcagtcattt gttttatcct catcatttgt attggtagct accttttcat
    16261 tttaagtcag aacagaatga agcacaagat aaaaaaacta taattgtatg gggacagatt
    16321 ggctaaaaat tatctgacct taacttaagg ccagatagct cattcatttc tatttttcag
    16381 ccgtaagggc agccattgtg atgtagttgt atggtttgtt gaagtgtggc aagaagaaga
    16441 ggtctgtcaa tgccaattta tcaattgtca catgctcttg gatagcaagt gagaacatgt
    16501 ggattcccat gctaattgca atatcatgtg aaaccatttg ggcaccaaga atttcacggc
    16561 tatctttgtc aaagacaatc ttaattgcta cttcatggtt gtcatgtttc atgaattctg
    16621 gtttttgaag atcgttaaag cctgtttcag ttgcgttgta accagcagct ttcgcttttt
    16681 caagagtcaa accagttgaa accatgtgaa gaccgtagat tgagatacca tttgaacctt
    16741 gaacaccgat tccttccaat tcatgtccac aagcattgta ggcaccaacg ataccagtgc
    16801 gaacagcatt tgaagcaaga gcgatatagc ttgtatcttt acgagcattg tcataaacag
    16861 tcgcacagtc accaacagcg taaacgcctg ggatagatgt ttcttgtttc ttgtctacaa
    16921 ggaaggcacc gttgcggaag agttcgatct taccatctgc aagggctgtg tttggacgga
    16981 aaccaactgc aaggataacc atatccacgt caaagctttc tttgtcagta atcaagcgtt
    17041 caactttacc gtcaccttcg attgctttaa cagtttgacc tagagccaag cggatgttgt
    17101 gatcttccaa gttcttcgcc atcatttgtg tgaagtcttt gtcatagtaa ccgttcaaga
    17161 cagtatcaac gatatcaaca aggacaactt cttttccaag acgttcaaag gcttcagcaa
    17221 gttcaacacc gatgtaacca ccaccaacaa cggcgatacg gtcgaggtgt tggctcttgt
    17281 cagaaagttt attgataact tcttcagcat tttggtacaa tttcacgaat tgtacgtttt
    17341 caagagttgc tttaaattcg cggtttcctt taacaatttc aacaccttcg attggtggca
    17401 agattggtgt agaacctgta gcgaaaatca atttttcgta tgattctttg tgctcttttc
    17461 cttcaacttc cgctgtaact actttgttat catagtcgat tgaaagaaca ggtgagttca
    17521 tgtaaacttt agcaccttta gcttccaatt tttctttatc agaatagaac aagccttcag
    17581 caccgtcaat ttgttcacca atccaaagag ccattccaca tcctaggaaa gagatgttag
    17641 agttttggtc aaatacaaca atttcgttct catttccaaa attatccaac atggtattga
    17701 tacatgctgt accagcgtgg ttagcaccga ctacaacgat tttactcata aaaatttcct
    17761 gccttcattc taaatttacc ttactagtat accatttacc gttagcgttt acaagggggg
    17821 tactattatt tcagcatttt ttatcaaaaa taattcccca tttatcctgt tttcaagcct
    17881 gatgaatttt atgagcatat ttcttgactt tttccaaatt ttgtttgtga aaattctaac
    17941 tttatggtat tataataaca tgaaaatgaa atgtaagggc ttcaatttag atgattattt
    18001 gatattacaa cttattttct ataagagaaa ggagttggta gcttgactct tcgttcacat
    18061 tctgaacggc taatggggac tactatcact atttcattag tggatgagca agccgatatc
    18121 tttctccaaa aatcctttga cttgctcaaa gaacttgaat accgcttcaa cgccaatagt
    18181 caagaatctg agttgatgga aatcaattat caagcaggaa tagcccccgt cacggttcat
    18241 ccagacctgt ttgagctgat ttcacttggg ttagagcata gcctagcgct ctctagccac
    18301 ctcaatatca gcattggtcc cttaattcaa acctggcgta tcggtttttc agatgccaag
    18361 gtcgcgcagc ctcaagaaat tgaatcggtg ctgcctctaa tcaatcctca tggtatcgag
    18421 ttagattctt ctacttctac tgtgttttta aaacagaaag gaatgaagat cgatctaggt
    18481 tgtttagcca aaggatacag tgcggataag gttgcccaat ttcttaggaa agagggggtg
    18541 acttctgcct tgatcaatct gggagggaat atcctgacca ttggaaaaaa tcaggcaaga
    18601 ggggataacc catggcaaat cgggattcaa gacccagcca atcctagggg aaatcattta
    18661 atgaccatcc ctgttgtcaa taaatctgtc gtgacttcag gcatttatga acgtcacctg
    18721 accgtcgatg gacaagatta ccatcacatt tttgacagtc aaacaggata tcctgttgaa
    18781 acggaactag cgagtctaac aatcatctct gataaatcag tcgatggcga aatctggacg
    18841 actcgactct ttggagaaag acctgcttct atcctctggc aagtcgaaag tttggagggc
    18901 atcgaagtca tcctcatcga taaagaaggc cacctaagct gttcttcagg aattcctact
    18961 ctatagagac aactgtttca atggaatttt aaaattgtat tatgtaaaaa gagaaaggaa
    19021 atctcatgtt aaaacttatt gctattgttg gaacaaattc aaaacgttct acaaaccgtc
    19081 aattgcttca atacatgcaa aaacactttg ctgacaaagc tgaaattgaa cttgttgaaa
    19141 tcaagaccat tcctgtcttc aacaaaccag ctgacaagca agtacctgct gaaatattgg
    19201 aaattgctgc taaaatcgaa gaggcagatg gcgttattat cggtactcct gagtatgatc
    19261 actctattcc agctgttttg atgagcgctc ttgcttggtt gtcttatggt atttacccac
    19321 ttttgaacaa accaatcatg attacaggtg cttcttacgg tacgcttggt tcatctcgtg
    19381 cccaattgca acttcgtcaa atcttgaatg ctcctgaaat caaggcaaat gttcttccag
    19441 atgaattctt gctctcacac tctcttcaag catttaaccc aagtggcgac ttggttgacc
    19501 ttgatgttat caagaaattg gatgccatct ttgatgactt ccgtatcttc gtgaagatta
    19561 ctgagaaatt gcgcaatgcg caagaattgc ttcgcaaaga tgctgaagac tttgactggg
    19621 aaaatttgta agataggaga ccaaaaagaa tgaaatttgt tggacttgtt ggatcaaact
    19681 acgatcaatc atataaccgt aagctcttgg aatttatccg tcgctacttc aaactcaaat
    19741 ttgaattaga agttcttgaa attgacgaag ttccaatgtt taaccaagat gaaaaatggg
    19801 atgaaagttt ccaattacgt tatttatata acaaaattac tcgtgcagat ggtgttatca
    19861 ttgctactcc tgagcataac cacactatct cagcttcact caaatctgta cttgaatggc
    19921 tttcatacga agttcatcca tttgaaaaca agcctgttat gattgtgggt gcatcatact
    19981 atgaccaagg aacatcacgt gctcaagttc accttcgtaa aatccttgat gctccaggtg
    20041 ttaatgccta cacgcttcca ggtaacgaat tccttcttgg taaagctaag gaagcttttg
    20101 ataacaacgg aaatatcacc aacgaaggca ctgttaaatt ccttgaaact tgcttagata
    20161 actttgttaa atacgtagga gtcgtttcga aattgaaaaa accaaaacca atcgaatcag
    20221 aagacttgga ttgtggaaaa ccaatcgcta caaccatcac agaagttgac cctgacgatc
    20281 cagaatgggt agaaaaagtt gcagcattca ctggtgctgt ttctggtgat acctatgtca
    20341 aattggacca cggtatccta acagttaacc aaattgatat gttcttgaaa gctatgccat
    20401 ttgaattgac atacgctgac gacaacaacc aattcctcta ctacaacaac gctcaccaag
    20461 atccagacac tatgttcgct aaacgtgtac cacctcaatc aggtagccgt atgtcgactg
    20521 ttcatggttc tcttccacca gcacgtatga agaacgtaga gtgggttatc ggaacacttc
    20581 gcaacggaaa ccaagaatac gtccgtacga tcgttccagg ttctcctgca ggtgttatca
    20641 acactcacaa ctaccaagca atgtactatc ctgatggatc atacgctggt atcaatgaaa
    20701 tcgtctttaa cttccaacca tggcttgact ggtacctaaa agagactggt caacgtttgg
    20761 tgggtggtag cggaccattt gctcctgctg ccggaggtca tggagacgcc gatgctactt
    20821 ctggcgcttc tgattcaggg gatgctggag gccacggtgg tgacgcagac gctacagccg
    20881 gtgcaagtta ctaatgatag tttttaggaa ccattatggt tcctttttta gcgaaaaaag
    20941 actagcaaac acaaactgct agtctctttg ttatcgtttc atctatcttt gttaaaagaa
    21001 agaatacata tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgtac
    21061 ttaagtacgg caaggtgaag ctgacgtggt ttgaatttga ttttcgaaga gtattattat
    21121 gaatttggat catcaagact acggccatgt aatccttttt cacgttgtac ttggcgtagt
    21181 ttttctggtg taacatcgtt tccttcttcg tccacaattt tgattccttc aatgtggtga
    21241 cgaacagcgc ggcgataacc ttcgatgtac tcctcacgta gtttggcttg ttccactttt
    21301 tcttctggtg ttaagccttc tgtttttttc tttttagcaa gctcattgat acgagcaatt
    21361 tttttcggat ccatttcttc tcctctgtaa taagataaag tccgtttatc aaattttatt
    21421 tagtgttaat ttggttaaaa caagcaaact tagctgcttt cttggttagt tgtgacaaga
    21481 ttgccaaacg attttgacgg acagcctgat cttcagccat taccatagta ttttcaaaga
    21541 aagcatcaat gactgggcta agcgcaaaga gttgtttcaa ttgctgactt gcaggtcctg
    21601 ataaaacgag tgtttctact gcttctgcca aagctttttc ttggtcattc tcaaatagtg
    21661 ctgaatcaac cgtagcaacc ccttctgcct tctcagccag gttaaaggca cgagaaagtg
    21721 attcaacaga tggtttaaaa tcttcttcct tgcttacttc tacgagagca cttgctgctt
    21781 ccaacatatc tgccacaaca aagtttgaac ctgcaagaac tgcttctttg atatcttttg
    21841 gagtagagcc catcatctta tcaacacgag ccttgataaa gtccataacc tctgctttat
    21901 tttcataagt caaactgtca aatttcaatg cataaaggct atcaatcagc tcatccatag
    21961 caatgtgcca accaaaggca tccaagatac gaaccacacc ttgagttgca cgacgaaggg
    22021 cataagggtc attagaacct gatggaatca atcctactga gaagaaactc aaaatcgtat
    22081 ccaatttgtc tgcaatggct agaactgcgc cgaccttgct ctctggaagt tctccttcag
    22141 ctgatgtagg catgtagtgt tcacgaatag cagctgccac cgctggagtt tcaccagcaa
    22201 gaagggtgta tttttcaccc ataattcctt ggagttcgtc aaattcacca accatacctg
    22261 tcaacaagtc aaacttgtaa atggctgctg cacgagcaag gtcaactgtt tcatccactg
    22321 acaaactagc tttttctgcc aaaagtacag tgatttgacc cgtacgaatc atgtgttcac
    22381 gaagggaacc aatcttctca tggaaggtga cattgtttaa tttttcaaca agatctgaaa
    22441 tcaccaattt ttggtcttca cgccagaaga attctccgtc ttccaagcgg gctaccaaga
    22501 ctttttcatt tcctttgatg acatttttca aacgctctgc gtttccgtta cgaacagaaa
    22561 tgaagtttgg caagagtttt ccatcttgat cacgaacaac aaagtaacgc tggtgttcct
    22621 tcatagaagt caccaagact tcttctggaa cttcaaggta tttagcatca aaacttccca
    22681 tgaaggcagt tgggtattca accaaattca agacttcatt cagcaaatcc gcatcaattt
    22741 cgatacgtac accatgtttt gcctcaattt ccttgatttg gtcaacaatc atttgctcac
    22801 gttcacatgg atctgcgatt acaaactgct tacgaaggtc ttcttcatag ctcaatgctg
    22861 actgaatttt ggtttctttc cccaagaaac gatggccacg gctcacacga cttcccttga
    22921 tatcaaggaa atccaagtca aactcttgct catccaagag aacagttaaa gtgtgaacag
    22981 ggcggatgta ttcaaagcta tttccagccc agtgcatgct gacagggaaa gtcaatgact
    23041 tcaagacatc tacaacacca ggaacaatgg cttcaactga ttggccaact tcttccttag
    23101 tgacatagac atattcttca cccttaattt cacggaattc gatatcttca acagtcaaac
    23161 ctttcccacg gacaaatcct tgagctgctt tggtgaagtt tccatcacta tccaaggcaa
    23221 ttttctttgc tggtcccttg aaatcttctg tcaaatcaga ctgtttgtct gataaaccag
    23281 tcacacgaac agccaaacga cgtggtgttg agaaggtttg aatggcttca aaagacaggc
    23341 ggttttcctt gaggaaggct gccatttttt cgcctagttg tttttcactt ggtgtgacaa
    23401 cataggctgg taattcttca agaccgagtt ctactaataa gttttttgtc atgttttttc
    23461 tccgaaaagt atctttttat tttccagttt gccttatgtg attggcacgc aagcaaccaa
    23521 cttcgttgtt tcattgctaa aattggagcc aaaagtctcc aattttgcct agtatcctta
    23581 gtttcactaa ggataccttc atcactgcgg caactggctc agggctcact ctgttcgccc
    23641 attttcgtaa tttgtcactc tctttattct gcgtcttctg ctaggagttt agctcttgtt
    23701 tcttcatcca aaagtgggta gcctaggcgt ttgcgttctg cgacaaaggt tttggctaca
    23761 acacgggcca agttacggat acgagcgata tagcctgcac gctctgttac agatacggca
    23821 ccacgcgcgt caagcagatt aaaggtatgt gaacatttga gaacatagtc ataggcaggg
    23881 tgtaccaagc cttcttctaa tgcacgacca gcttcttttt caaacttatc aaagttttca
    23941 agcaacattt cttggtccga aatttcaaat gaatattttg agtgctcata ctcaggctgg
    24001 ataaagattt ctccgtattt tacaccatca gcccactcga tatcatagac agagtctact
    24061 tcttgaatgt aagaagccaa gcgctccaaa ccataggtaa cttccgcagt cacagggcca
    24121 gttgccaatc caccgacttg ttggaaataa gtgaactgag tgatttccat tccgtcaagc
    24181 caaacttccc aaccaagacc agctgaacca gttgatgggt tttcccagtt gtcctcaaca
    24241 aaacgaatat cgtgctccaa aggattgatt cccaattttt ccaaagactc aaggtaaagt
    24301 tcttggatat ttgatggaga aggcttcatg accacctgga attggtggtg ttggtagaga
    24361 cggttagggt tttccccata acgaccgtca gcaggacgac gtgatggctc tacataagct
    24421 gcattccatg gctcaggtcc gatagcacga aggaaagtgt aaggactcat tgttcccgca
    24481 cctttttcat tatcataagc ctgcataagc atacaacctt ggtcattcca aaattgttgc
    24541 aaagtaagga tgatttcctg gaaagtcaat ttcttagaca ttgtttactc ctttttatat
    24601 aaattacttg cgacatgagt ctgcctcgtc gatcatacga ggcaagacga gttagtactg
    24661 cgtctagctc aggcactcta gtgctgagcg tggggcagtc cgactgtaag gaggtttctg
    24721 ccactgacgc agtgaaatgt caatttctta gacattgttt actcctttag tttttatatt
    24781 tctttttcaa ttaatttctg ctgaacaaac caatctaaca agggaaaagt tttttctgta
    24841 tttgcccata aatgataaga atctaagacc gcttgtacac tacctaaatt ttctaccaac
    24901 ttatctactg tcaaaaagct gcgccagtat tcataaaaaa tacttgcata gttcttatca
    24961 taagtcgaag aaccaaaatc attcaatgaa tgccaaccat acttcttctg aaaaagttct
    25021 acgagagatt gattacaaat tttttccgct tgaaattcct cttctgtcaa gaaatacttg
    25081 cgactaatat attcaaccat cccctcttcg aaccagatat aagagtcata accatcaaaa
    25141 tcatctaaaa aatgctccga ccaatgagct aactcatgtc ctacaatctg taagaggaaa
    25201 ttttcagata aagaatgata gtgactttct attgcttgag tttgctgaga acactcgtag
    25261 tccatcaatt gatacaaata caactctttc caaacagcta aatcaggcgc cataaccatt
    25321 cgtctattat ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta
    25381 aaatctgacc aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa
    25441 gattgataat ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt
    25501 tgactatcgt cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag
    25561 actaaataag caaaagaacc gcgtccgaca atcctaggtt gatttaacct aggggcgtca
    25621 gaaacgcggt tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc
    25681 cagctcatta ttttgcccta cttggctccc actatcccaa gctcgcttga agaacgccat
    25741 aagactttct ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta
    25801 tgattttcta gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt
    25861 ttaagttttc tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa
    25921 tacgagaggt tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccaggcca
    25981 aggctatctg agcaatcgat tttccgtgat ttgctgcgat ttcttgcact tgcttgctat
    26041 caaatagttc tccctgacca aaaggccccc aagcttccaa taaaattccc ttttcacgac
    26101 agtaagctac gacttgatct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag
    26161 gaacgatagt tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc
    26221 cgatagcacg gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat
    26281 tgcgagtttt ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat
    26341 acaaatccaa ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt
    26401 gctcataggt ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa
    26461 ctccgctatc tttgattgct tgaccaacac tttcttcatt ctgataaatc gccgccgtat
    26521 caatatgacg ataaccagcc ttcaaggctt ctaacactgc atgataggct tcttctccat
    26581 ccttagcctt aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat
    26641 aggtattcat atgcttctcc ttctatttta ttcatcaatt atagcataag acatttttta
    26701 agtagtacta tcttctgtaa aaaataagtc tgtcaaatat ctaacagacc tattttttct
    26761 tgcttattca tcacgactat agtacagctc atgagctttt aggcgagtat tgagcatctc
    26821 tggtatggtc acaaaggtat aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc
    26881 attgactgtc ggactgtgaa tatcatgcat caaaacgata gagccgttag ctacttgatg
    26941 ctgaatttct gtcaaaatag atgcttcatt tttactcttc cagtccagac tatccacatc
    27001 ccacatgata aagctcaaat ccaagctatt gcgaatatca tctgtaatag caccataagg
    27061 tggacgcatg agtttagaac tagaacccag cactttagtt agcacatcct cagtatcagt
    27121 aatctgcttt ttagcttcat caagagagag ttgcgagaga atcggatggc tccaactatg
    27181 gtttccaaca acatgacctt cagattttat cctcttcacc aagtcctcat tcccagaaac
    27241 atttttccca agcacaaaga aagtcgcttt aatatcatat ttagctaggg tctctaatac
    27301 ctgcggggtc gttgctggat ttggaccatc atcaaaggtt agagcgacaa ctttttgata
    27361 tttcttatca aagtaagatt ggtacaaggc cgcatctttt tcgagtaagt acgaagattg
    27421 gataacatca aagaaagcag atactggcaa ggctatctct tctaaatttt caagcacagg
    27481 acttggataa aggataatct gactatcctt gtaatcaaaa ttccatgcag acaagtcttg
    27541 gtcagagaag ttttttacaa tctgctcact ctggtcttgc tctatttttt tatcctctat
    27601 gaagggggtc aactctttta tcagctgttc cttagcctta ctagcatctg aaaacagttg
    27661 gtcaagtgta aaaggttgcc catcttctgt taagtgaacc tttcctagac tggtcttttc
    27721 agtctcttca accttaaaag caactaaatc atagatttgt ttggtcacat tacgattaac
    27781 gactcccttt aaacctgact cttcttgctc tgtatagtag aaaacaagat tgtccttact
    27841 ttctagcttg tccttaacat cttgatttat caactcccta acagaggaaa tcactttctc
    27901 cccttggaga ggataatagg caatcacttc ggcttgcccc tgacgaaaat gctccttctg
    27961 atttccctca ctcaattgat catctttctc ttttttgagc gattcaatct tttgctcaaa
    28021 acttttttgc tggtagatct taaaagcaat aaatccgcct aataggcaaa tagaaattgc
    28081 taaaatacca atcaaagcca ataatacatg tctcgttttc ccgtgtctgc cacgtcctag
    28141 tctactttta ttcatattta tatcataaca aatttgagca ataatttcaa tgatacaatt
    28201 tggcaatcca caattcctct agcaagccaa agcatcttac atcaactcca acatccgtat
    28261 ctgtagagat tatgtagttt tctatctatg gaatttctag tagattgaag ctagaatagt
    28321 agacctatac ttctaaaaca ttgttagaaa tcgatttgat tgtcctgatc gatttgtcct
    28381 gttcttattt cattttacta taaaactatg agaagctagg tcatctgaga agtaattact
    28441 atcgacaatt gcgatagcgt atactggtgc gatgtgtttg tagctctggt gggtatcacc
    28501 ttcacgttgg cgaatttttt ctaggttttg attaacctga ctgcacagat aagcccatag
    28561 gcgattgatg aaaaaattct gatgatgaac ctgaatttcg atgataactt ggattccatt
    28621 atctaactcc gccaagacgt ctatactagt atagaaatcc tgcgctgagt agggcatgga
    28681 aggcaagacg tgaatgttac ttccctccaa aatcgtcaca ttttttgctg gcaagtccag
    28741 catatcgcgg ataaattgac aagtgatttc tggattgctg aatatttttt tagcaaccaa
    28801 atcattagtt gggctgatgc ccggatgtct gagaatcctc cttctattat agcacatttt
    28861 taaatctagg tttgctagat tctatgcttc tatctattta ttcggaaaag aaagctaata
    28921 gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt tataatgaaa
    28981 gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat cggaactggc
    29041 gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca actggtcgca
    29101 atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca gaatatccaa
    29161 ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta tatcgccagt
    29221 ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa acatgttatt
    29281 ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca aacagctgaa
    29341 aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc ctttactact
    29401 atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta tgccaaatac
    29461 tcttccaaga tgcctgactt gttgactgga caaacaccaa atgtcttttc aaaccgtttt
    29521 gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt tcgtcttttt
    29581 ggaaaagcca atgacgcgac ttaccatgct caacagcttg acaatagcat tgacctaaat
    29641 ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg aaaaaacatc
    29701 acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct caacacgatt
    29761 gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca gcttcctatc
    29821 caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat gatccagcaa
    29881 ccagatctga atctctacca aacttggctg tatgatgcag gctctgttca tgagctacta
    29941 tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa accaaactac
    30001 cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc cccattcaaa
    30061 ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc caaaccggaa
    30121 ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag aaaaaagccc
    30181 aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt gatgtatgta
    30241 aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt tcaagtcaga
    30301 aacgacaaat tgaacgcctt aaaaaggggc cagaaattct gattggaact cctggccgta
    30361 tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc atcatcctgg
    30421 atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa atcactcact
    30481 acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt gatcaagaaa
    30541 agattgtgcc gaatacacgc actattgatc tctctgatca aaaattggac aatatccagc
    30601 atttctacat gcaggtagac caacgtcatc gagtggatat actacgaaaa ctggctcatg
    30661 tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg aatgccgagg
    30721 aaaaactaca gtatcgtgat atattggctg tttccctcgc tagtgatgtt aatgttaaat
    30781 ttagaaaagt catcttagaa aagtttaaag ataaccaact aaccctgctt cttgcaactg
    30841 accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac tttgatattc
    30901 ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg ggcaaggaag
    30961 gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa aagtttgcaa
    31021 gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc cccattgctc
    31081 tatacacatc cagtgtatgg agctttttta taagcaattc aattttatac tcatctgctt
    31141 tcaaaaagca ttctattcca tctccgatta acgatggact ttatcacctc cttctccagt
    31201 ccttgtatga catcttggag ttgattcatg acatcttcca aagttcgaaa ggctttattc
    31261 ttaaatccac gtttacgaat ctctttccac acttgttcaa tgggttcatc tctggtgtgt
    31321 atggaggaat aaaggtaaaa tcaatattag tcggaatctt taaggtactt gatttatgcc
    31381 atatagcatt gtccataacg agtaaaaaat aatcatctgg ataagcttgt gaaagctctt
    31441 ctaaaaagac gttcatccac tcagtattac atccaccagc tattaagaaa aatgattcgc
    31501 ctgtatgggg catcaacagc tccataacaa tagcgaaatt ctcgtatata gtgactatgg
    31561 acatgtggac ctactcctat tggagaccaa caagatccca gtttactgat tctaccgaaa
    31621 ccagcctcat cttggtacat caagcgaacc ttatgaaaag gtctactggt tttaaagcgc
    31681 tttcttgtct tcttgaattg agattttatt tttagacgcg acaatggttt gagcgtctgc
    31741 tttcttagga tgttctggac gtggcataat atttcgccaa ccatggtgct tcaacagttg
    31801 atagaaggca tcacgtgtgt aggaacgata taagcctgaa ataaggtatt gtaacaaatt
    31861 ctcctgcctc tgtagccttc aaatggcggg caagaaaggc tttctcttcc tcaactgtca
    31921 tatatgcatg gttacgacca ccacgtgttt cttgaaggag agagtcgagt ccgaactcct
    31981 catatttttt tacatttcgc caaatcgttg tttgattaca gtctaaaaga tctataatct
    32041 ctttataaga tttgcccatc agacgaaata gaacgatttg aaggcgttta tgatatttag
    32101 ctgtacgaaa gtcttttaaa agtgttttga tggtttggat ttcttcttca gttgatttca
    32161 tattactatt gtataatgct ttttgatttg agtctagtat tatactcttc gaaaatctct
    32221 tcaaaccgcg tcagcttcgc cttgtcgtat agatgttact gacttcgtca gttctatcta
    32281 caacctaaaa acagtgtttt gagcagtctg cagctaactt tctataacaa aatgaaataa
    32341 gaacaagaca aatcaatcaa gacaatcaaa tcgatttcta acaatgtttt agaagtagat
    32401 gtgtactatt ctagtttcaa tatattatag tttgctcttt gattttcatt gagtattagt
    32461 tcaatatcta aaaaaagagg ctctataata tttgtagtgg gtaaatcccc tat
//
LOCUS       DAVMMR010000022        31542 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMMR010000022 DAVMMR010000000
VERSION     DAVMMR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.022"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..448
                     /locus_tag="VYS74_000978"
     CDS             <1..448
                     /locus_tag="VYS74_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3789034.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
     gene            865..1245
                     /locus_tag="VYS74_000979"
     CDS             865..1245
                     /locus_tag="VYS74_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV3789035.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            1705..2418
                     /locus_tag="VYS74_000980"
     CDS             1705..2418
                     /locus_tag="VYS74_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV3789036.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2432..3079
                     /locus_tag="VYS74_000981"
     CDS             2432..3079
                     /locus_tag="VYS74_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789037.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSN
                     FDDFLEKNYLKTIWQVSKETPFSEMDFNIFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3217..3870
                     /locus_tag="VYS74_000982"
     CDS             3217..3870
                     /locus_tag="VYS74_000982"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV3789038.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3880..4344
                     /locus_tag="VYS74_000983"
     CDS             3880..4344
                     /locus_tag="VYS74_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3789039.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4357..5625
                     /locus_tag="VYS74_000984"
     CDS             4357..5625
                     /locus_tag="VYS74_000984"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV3789040.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5639..5899
                     /locus_tag="VYS74_000985"
     CDS             5639..5899
                     /locus_tag="VYS74_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789041.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5910..7271
                     /gene="pdrM"
                     /locus_tag="VYS74_000986"
     CDS             5910..7271
                     /gene="pdrM"
                     /locus_tag="VYS74_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV3789042.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7425..8393
                     /locus_tag="VYS74_000987"
     CDS             7425..8393
                     /locus_tag="VYS74_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV3789043.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8409..9401
                     /locus_tag="VYS74_000988"
     CDS             8409..9401
                     /locus_tag="VYS74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV3789044.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            9619..10662
                     /locus_tag="VYS74_000989"
     CDS             9619..10662
                     /locus_tag="VYS74_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV3789045.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10726..12411
                     /gene="lpdA"
                     /locus_tag="VYS74_000990"
     CDS             10726..12411
                     /gene="lpdA"
                     /locus_tag="VYS74_000990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV3789046.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12475..13464
                     /locus_tag="VYS74_000991"
     CDS             12475..13464
                     /locus_tag="VYS74_000991"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV3789047.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13529..14599)
                     /gene="xerS"
                     /locus_tag="VYS74_000992"
     CDS             complement(13529..14599)
                     /gene="xerS"
                     /locus_tag="VYS74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV3789048.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(15203..16753)
                     /locus_tag="VYS74_000993"
     CDS             complement(15203..16753)
                     /locus_tag="VYS74_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV3789049.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16769..17548)
                     /locus_tag="VYS74_000994"
     CDS             complement(16769..17548)
                     /locus_tag="VYS74_000994"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV3789050.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17535..18386)
                     /gene="ylqF"
                     /locus_tag="VYS74_000995"
     CDS             complement(17535..18386)
                     /gene="ylqF"
                     /locus_tag="VYS74_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV3789051.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(18640..23853)
                     /locus_tag="VYS74_000996"
     CDS             complement(18640..23853)
                     /locus_tag="VYS74_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3789052.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            complement(23897..30082)
                     /locus_tag="VYS74_000997"
     CDS             complement(23897..30082)
                     /locus_tag="VYS74_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3789053.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGINTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEQVAPLPEYTGVQAGAIVE
                     PEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTG
                     VGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIH
                     FYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLK
                     DATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPT
                     QLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGN
                     KYRHVTSLASLPSNAENYFMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLI
                     QHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELP
                     DGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDT
                     ATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVAS
                     YQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGN
                     LNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGT
                     YSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTEDFNFSS
                     RYVTDYKNLENADSSKEKVYKNIEKLLPFYNRETIVKYGNLVETSSNLYNKELLSVVP
                     MKDKEVISDINKNKSSINKLLLYYADNTSETLNVNYQTDFSNVAEYRIGGTNLIYTPN
                     TLLRNYQNILDEVLPALNSVEYKSEAIRKVLDVSKDVSLTELYLEEQFNTTKTNLKDS
                     LTKLLTADAAIAENNNKVIDNYVIEKIKNNKEALLLGLTYLERWYDFKYRDTKAKDLV
                     MYHLDFFGKSNSSALDNVIELGKSGYNNLLAKNNVITYNVLLAKNYKTNNLFDALEKY
                     RKAFVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSPSWKY
                     PSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRF
                     RDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFETNHPAIKHFFG
                     PAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNG
                     LGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYM
                     HNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPE
                     ELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMG
                     RRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLSKVFNN
                     QYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVK
                     FDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            complement(30291..31469)
                     /locus_tag="VYS74_000998"
     CDS             complement(30291..31469)
                     /locus_tag="VYS74_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789054.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         9290 a         6009 c         5892 g        10351 t
ORIGIN      
        1 aagaaacaaa aatggtcaag ctgataccaa tcaaacggaa aaaccaaacg aggagaaacc
       61 tcagacagaa aaacctgagg aagataagga acatgatgaa gtaagtgagc caactcaccc
      121 tgaatctgat gaaaaagaga atcacgctgg tttaaatcct tcagcagata atctttataa
      181 accaagcact gatacggaag agacagagga agaagctgaa gatactacag atgaggctga
      241 aattcctcaa gtagagcatt ctgttattaa cgctaagata gcagatgcgg aggccttgct
      301 agaaaaagta acagattcta gtattagaca aaatgctgtg gagacattga ctggtctaaa
      361 aagtagtctt cttctcggaa cgaaagataa taacactatt tcagcagaag tagatagtct
      421 cttggcttta ttaaaggaga gtaagtaaag gtagcagcat tttctaactc ctaaaaacag
      481 gataggagaa cgggaaaacg aaaaatgaga gcagaatgtg agttctagtt ctcatttttt
      541 tcatgaaaat gtgcaaaata tagtagattg aaactagaat agtatacctc tacttctaaa
      601 acattgttag aaatcgattt gactgtcctg ttcttatttt actatatctt aacagatagt
      661 gtaaataaag ataaactatt tactggctaa ttaatcagtt aaacactagt taaggagtaa
      721 tgatgaaaaa aagaacaata ctattattga tgaccagtct gttggctctt gtcttaggag
      781 catatggttt cttggacata ttgatcctgg atcattctca tcaggattac tctttactgc
      841 tattttagaa actggggtgg tttgatggaa agtattggta ttggtcttgt tatcgtttca
      901 cattccaaac acattgcaga aggtgttgtt gaactgatta gtaaagtagc taaagatgtt
      961 ccgattactt atgtaggagg aaccgagggc ggaggaattg gaacgagttt tgatcaagta
     1021 gatagggttg tttccgaaaa tccagcagat actttacttg ccttttttga cctaggttct
     1081 gctaaaatga acttaaaaat ggtgactgat ttcagtgata aaagtatcat catcaacagg
     1141 gttccaattg tagaaggtgc ctataatgca gctgctcttc tgcaggctgg tgcagaactg
     1201 tcagttattc aaacacagtt agcggagctt gaaatcaata aataaggaat tttactataa
     1261 ctctttttat aggtaagcta ttgattatct caactataaa atgttaagta aatgatttca
     1321 aaattagaaa gggattgctt tatgcagttc ctttttattt taacaggagt gaaactatag
     1381 tgtttctaaa ttgtgaatca atcaaaactg attgtgatgg ggctattcta gctttagaat
     1441 ccttcaaaaa ttaaaattta aggcaatcaa ttacttggaa gagtatgaaa gcatttagtt
     1501 tataggaatt ctaggtctag aattacacat atatttatga agacgggggt gctcgatagt
     1561 tagtattgtt ctattctgaa agatttgagc tgtcagttgt atagaaagtg ttcgaatttt
     1621 ttttaagtga ttaaattagt taattgtatg aggtgcttta tgatataatg ttcttaatga
     1681 attttcagaa aggaaaacct caaattgttc tacaaatttc tactcttcga cctcgaccac
     1741 actcttcttg attttgatgc tgctgaggat gtggctttga cccaacttct aaaagaagaa
     1801 ggagttgcgg atattcaggc ttataaagat tattacgttc ctatgaacaa ggctctctgg
     1861 aaagacttgg agctgaagaa aatcagtaaa caagagctgg ttaacacgcg cttttctcgt
     1921 ttattttctc attttggaca ggaaaaagac ggtagttttc ttgcccagcg ttaccaattt
     1981 tacctcgccc agcagggaca aacactatcg ggcgctcatg atctcttgga cagcctcatt
     2041 gagcgtgatt atgacttgta tgctgcgaca aatggcatta ctgccattca gacaggacgt
     2101 ttggctcaat ctggtctagc accttatttc aatcaagtct ttatctcaga acagttgcaa
     2161 actcaaaagc cggatgctct cttttatgaa aagattggcc agcaaattgc tggatttagt
     2221 aaagaaaaga cgctgatgat tggagattct ctaaccgccg acattcaagg tggcaataat
     2281 gcggggattg acactatctg gtataatcct catcacctcg aaaatcacac acaagcccag
     2341 ccgacttacg aagtctattc ttaccaagac ttgctggatt gtttagataa aaatattctt
     2401 gaaaagatca cattttaaag gagattagct aatgactaca aaaaaagcta atattactat
     2461 tgaagagtac attgaaatgt ctgaagttga ttttaatgaa gctgttaatt atgaatttac
     2521 atctgacact tgtcaattag caaatagtat ttatcaatct ctttttaagt tttttgataa
     2581 gaaaaatttc tctggcgatt taatttttac ttggaaatct ccatcattag tcaaagaagg
     2641 ggattatatt gggagaaggg attcacaagt agataatctt agagtaatag gaaatatatt
     2701 tccgaattat cttactaatc gaaaatatag cctcaatatg aatcgtaatg gctgtatggg
     2761 agattttcct catgactttt ttgatatata cctagatcat gtagcaaaat atgcctacga
     2821 acaaaaagtt aataatatta aagagtatta tcctttaaaa agagcgattt tacaccaaga
     2881 gaatgcattg tattttcgat tgttttctaa ttttgacgac tttttagaaa aaaattattt
     2941 aaagactata tggcaagttt ctaaagaaac tcctttttct gaaatggatt ttaatatatt
     3001 taaaaatatt tcagaaaaga taatcttcga aagaggtagt aaaatgctta atgatttaaa
     3061 aagtaattat aaaaaatagc cagtctttat aaactgtatt cacgtattca aatcgtatat
     3121 atagtcgatt tattaaatct ttagataaac tgtaaaaagt ggtttccata gccacttttt
     3181 gctataatag aggcagtaaa aatgaaggag tcggctatgg aacactcgtc ttggcatgct
     3241 ttgattaagg cgcaattacc tgagggttat ttcgggaaaa tcaatcagtt tatggagcag
     3301 gtctattctc aggggattat ttatccaccc aaggaaaagg tttttcaggc tctcttgaca
     3361 acactgcttg aagaagttaa ggtggtaatt ctagggcaag acccctatca cggaccaggt
     3421 caagcgcagg gcttgagttt ttctgtacct gactctatcc cagctccacc atccttgcaa
     3481 aatatcttga aagaattgtc agatgatatc ggagttaaga aatctcatga tttgacagct
     3541 tgggctgagc aaggagtctt gcttcttaat gcttgtttga ctgttcctgc tggacaggcc
     3601 aatggtcatg ctggtcaaat ctgggagcct tttactgatg ctgtgattca ggtggtcaat
     3661 catctagata gaccagtcgt ttttgtactc tggggagctt atgcacgtaa gaagaaggca
     3721 ttagttacca atcctcatca cttgattatc gaatcagccc atccaagtcc tttgtcggtt
     3781 tatagaggat tttggggttc caagcccttt tccaaggcca atgcattctt aaaagagaca
     3841 ggacaagagc caatcgattg gcttagataa ggagaaaata tgcctcagtt agcgacgatt
     3901 tgctacattg ataatgggaa agaactgctc atgctccatc gtaataagaa acccaatgat
     3961 gtccatgaag ggaaatggat tggtgtgggt ggtaagctag agagaggaga gacgccccag
     4021 gaatgcgcgg cgcgtgaaat ccttgaagaa acagggctca aagccaagcc agttctaaaa
     4081 ggtgtcatca cttttcctga atttacacca gatttagact ggtacaccta tgtttttaag
     4141 gtgacggagt ttgagggcga cttgattgac tgcaatgagg ggacgctaga atgggttccc
     4201 tatgatgagg ttttgagcaa gccgacttgg gaaggtgacc acacctttgt tgagtggctt
     4261 ttagaggata aacccttctt ttcagccaag tttgtttatg atggggataa attgttggat
     4321 acccaagttg atttctatga ataaaggaga aagcagatgc tactaatcaa aaatggtcgt
     4381 gtaatggatc ccaagtctgg tttggatcaa gtttgtgatg tcttagttca agatgggaaa
     4441 attatcaaaa ttgcgtctga gatcacggaa gaaggagcag aaacgattga tgctactggt
     4501 cttgtggttg ctcctggctt ggtcgatatt catgttcatt tccgtgaacc tggtcaaaca
     4561 cataaagaag acattcatac tggtgcccta gcagccgctg caggtggttt tactactgtc
     4621 gtcatgatgg ctaataccag tccaaccatt tcagacgtgg agactttgca agcagttctc
     4681 cagtcagctg ccaaagagaa gattaatgtc aagacagttg cgaccattac taaaaacttt
     4741 aatggtaaaa acttgactga ctttaaggca ctcttagaag ctggtgcggt tggtttctct
     4801 gatgacggta ttccgcttga gagcagtaag attgtcaagg aagccatgga ggaagccaaa
     4861 aaactcaata cctttatcag ccttcatgag gaagatccag gtttgaacgg tgttcttggc
     4921 tttaatgaaa atattgctag agaacatttc catatctgcg gtgctactgg ggtggctgag
     4981 tacgctatga tggcgcgtga tgtcatgatt gcctatgcaa ctaaagccca tgttcacatc
     5041 cagcatttgt ctaaggaaga aagtgttaaa gtagtggagt ttgctcaggg gttaggtgca
     5101 gaagtcacag cagaagtagc gccacagcat ttctctaaga cagaagcact tcttttaaca
     5161 caaggtagca atgctaagat gaatccaccg cttcgtttgg aatcagaccg tcgtgccgtt
     5221 atcgaaggtc tcaaatcagg tgtcatcaca gttattgcga ctgaccacgc gcctcatcat
     5281 gtagatgaaa aaaatgttga ggatattacc aaagcgccat ctggtatgac tggcttagaa
     5341 acatccctgt ctctcagctt gacctattta gtagaagctg gtgagttgag cttgatggaa
     5401 ttacttgaaa aaatgacata caacccagcc aagctttaca actttgaagc aggttacttg
     5461 gctgagaatg gtccagcaga tatcactatt tttgatgcca aggctgaccg ctttgtggac
     5521 tcccattttg cttccaaagc agctaattca ccattcatcg gtgaaacctt aaaagggcag
     5581 gttaaatata ccatctgtaa gggacaaatc gtctatcaag cttgatagga gttagaatat
     5641 gaattatttt cgaaaacgta gggagagaca agcgaaatct aatagtggaa tttactgtcc
     5701 cgcagctaat cgttccaaaa ttcaatatga aactaaagaa aaagctgacc atgcggttca
     5761 atatgataac ggaggtttag ttagaagcta ttattgtcgt acatgtgcgt gttggcatac
     5821 tacttcaaaa tctaataaac ctccgctaag tttgaaaaac tatggtctga aactctttgg
     5881 tttagataaa tcagaataga aagagaagta tgtataagac aaagtgttta cgagagaagt
     5941 tagtattatt tttaaaaatt ttcttcccaa tcctgatcta ccaatttgcc aattattctg
     6001 cctcttttgt tgatactgca atgacaggtc aatacaacac tatggacttg gctggtgtat
     6061 ctatggcaac cagtatctgg aatcctttct ttacatttct aacagggatt gtgtcagcct
     6121 tggtgcctat cattggtcac catcttggtc gaggcaaaaa ggaagaagtt gcgtctgatt
     6181 tttaccaatt tatttatttg gccttgggcc tatctgtggt cttgctgggg atggtacttt
     6241 tcttggcacc aataatcttg aatcatattg ggttagaagc agcagtagcg gcagtagcgg
     6301 ttcgctatct ttggttttta tctatcggga ttatcccctt gttgctcttt agcgtcattc
     6361 gttccttgct ggattcgctg ggcttgacca aactgtccat gtacctcatg cttttgttac
     6421 tccctctcaa tagcggattt aactatctct tgatttacgg tgcctttggt gttccagaac
     6481 tgggaggggc tggtgctggt ttaggaacat ccttggccta ctgggtcttg cttgggattt
     6541 ctgttctggt tttatttaaa caggagaagc tcaaagcctt acaccttgag aaacgaattc
     6601 cacttaatat ggataacatt aaggaaggag ttcgtttagg tctgcctatt gggggaactg
     6661 tcttcgcgga agtggctatc ttttcagtgg ttggcttgat tatggctaag ttttcgtcct
     6721 tgattatagc tagtcaccag tcagctatga acttttcaag tcttatgtac gcctttccta
     6781 tgagtatctc atcggctatg gctattgtcg tttcctatga agtgggagcc aagcgatttg
     6841 atgatgcgaa aacctatatt ggtctaggaa gatggactgc tctcattttt gcggccttca
     6901 ccctaacctt cctttacatt tttaggggaa atgtggccag tctttatggt aacgacccaa
     6961 aatttatcga tttgacagcg cgttttttaa cttatagtct tttcttccag ttagcagata
     7021 cctttgcggc accgcttcag ggaattttgc gggggtataa ggatacagtt attccttttt
     7081 accttggttt gcttggttat tggggcgtag caatccctgt ggctacgcta tttgattccc
     7141 taacagattt tggagcttat tcttactgga tcggcttgat tattagtttg attgtgagtg
     7201 gggcgctcta ccgttggcgt ttaactgtga ttatgaagag atttgaatct ttagcaaaat
     7261 ccaaacgcta aaaagtttgt gaaaaaatat tcttgacaag aaaatccctt gttttcacgg
     7321 ggttttcttt tttattttgt gaaatttctt ttagaaaaac tttgacagta tttgccaaaa
     7381 gcggtaaaat agtaaggtaa gaagaaagtt agaaaggcaa gaagatgtca actttagata
     7441 aaaatctttt gctagaaatg ttccgtaaga tggaagaaat ccgtcgcatg gacttaaaaa
     7501 ttgcacaatt agtaaagaaa gggaaagtgc caggaatgac gcacttttct gttggtgaag
     7561 aggcagctaa cgtgggggct atgttagctc tcaatccaga tgatctgatt acctcaaacc
     7621 accgtggaca cgggcaagct attgctaaag ggattgacct caacggaatg atggctgaaa
     7681 tccttggtaa atacactgga acctgtaaag ggaaaggtgg atctatgcat atcgctgacc
     7741 ttgatgctgg gaaccttggt gccaatggta tcgtaggtgg tggtatgggg atcgctgtcg
     7801 gtgcagccct cagtcagcaa atgcaaaata ccggtaaaat cgttgtctgc ttctttggag
     7861 atggtgcgac caatgaaggt gttttccacg aagcagtgaa catggcttct atctggaacc
     7921 tgccagtcat tttctattgc attaacaacg gttacggtat ctctgcggat atcaagaaaa
     7981 tgaccaatat agaacatatc catcaacgta gcgccgctta tggaattcct ggaatgttca
     8041 tcgaagatgg taacaatgtc atcgatgtct atgaaggatt tcagaaagct gtggaccatg
     8101 ttcgcagtgg caatggttca gtcttgattg aaagtgtaac ttatcgctgg ctcggtcact
     8161 catcatctga ccctggtaaa tatcgtacgc gtgaagaagt ggaattgtgg aaacaaaaag
     8221 atccaatcga aaacctccgc aattacctta ttgaaaataa cattgcaagt gccgaagaat
     8281 tggaagaaat ccaagcgcaa gtaaaggaag cagtagaagc ttctgttaaa tttgcagagg
     8341 aaagcccatt cccaccgctt gaatcagcct ttgaagatat ttacgcagac taaggagaaa
     8401 gagataaaat ggaaacaaaa acaatgtcct tccgtgacac cattatcctt gctatgtctg
     8461 aggaaatgcg tcgcgatgaa aatgtgttct tgatgggaga agatgtcggt gtcttcggag
     8521 gagacttcgg tacttctgtt ggaatgcttg aagaatttgg tccagaacgt gtccgtgact
     8581 gtccgatttc tgaagctgcc atctctggag cagcagcagg agcagccatg acaggacttc
     8641 gtccaatcgt cgatatgacc ttcatggact tctctgttat tgccatggac aatatcgtca
     8701 accaagctgc taaaacacgt tacatgtttg gtggtaaagg tcaggttcca atgactgttc
     8761 gttgtgcagc tggtaacgga gttggctctg ccgcccagca ctcacaatct ctagagtctt
     8821 ggtttactca cattccagga cttaaggttg tggcacctgg aacacctgcg gacatgaaag
     8881 gcctgcttaa gtcttctatc cgtgataaca acccagttat tatacttgag tacaagtcag
     8941 aatttaacca aaaaggggaa gtgccagttg atccagacta cacaattcca cttggggttg
     9001 gtgaaatcaa acgccaagga acggatgtaa cagttgttac ttatggaaaa atgcttcgcc
     9061 gtgtggttca agctgctgaa gaattagcag aagaagaaat ttcagttgaa attgttgacc
     9121 cacgtaccct tgttccgctt gataaggata tcatcataaa ctcagtgaag aagactggta
     9181 aggttattct ggtcaacgat gcccacaaaa caagtggcta tatcggtgaa atttcagcta
     9241 ttatttcaga atcagaagca tttgactatc tagatgcacc aatacgccgt tgttcaggag
     9301 aagatgtgcc gatgccttat gcacaaaacc tagaaaatgc aatgattcca acagttgaaa
     9361 gtatcaaaga tgcaatccga aaaacttata acaaagacta gagttacata atataagtta
     9421 tgtaactaaa ataaagctga cgagaaactt tgtatctttt aggtgcagag ctcttcgtca
     9481 ttaatatctt atattcgagc acgggctaaa agctcggaaa aaagataaat ctccttggct
     9541 tcatcgaagc catcgtcgat ttcctatttt tcagtcgctt ttgacgccct ttgtatcata
     9601 aatagaattg gagaggtcat ggctgatgac aagctaagag cgactcctgc agctagaaag
     9661 ttagcggatg atttagggat aaacctctac gacgtttctg gctcaggtgc aaacggtcgt
     9721 gtccacaaag aagacgtgga aacttataaa gacacaaacg tggttcgcat ttcgccactt
     9781 gcaaaacgaa ttgccctcga acataacatt gcttggcagg aaatccaagg aaccggtcat
     9841 cgtggtaaaa tcatgaagaa ggatgttttg gccctgcttc ctgaaaatat cgaaaacgat
     9901 agcatcaaat ctcctgctca gattgaaaaa gtggaagaag tccctgataa cgtaacacca
     9961 tatggtgaaa tcgagcgtat tccaatgaca ccaatgcgta aggttattgc ccaacgcatg
    10021 gttgaatctt acttgactgc gccaaccttc accctcaact atgaagttga tatgactgaa
    10081 atgttggctc ttcgtaagaa ggttcttgag ccaatcatgg aagcaactgg gaagaagact
    10141 actgtaacag accttctttc gcttgcagtt gttaagactc ttatgaaaca cccatacatc
    10201 aacgcttcat tgacagaaga tggcaagact attatcactc acaactatgt caatcttgcc
    10261 atggcagttg ggatggataa tggattgatg acacctgttg tttacaatgc tgagaagatg
    10321 agtctttcag aactggtggt agcctttaag gatgttattg gccgtacctt ggatggtaaa
    10381 ttggctccaa gtgagttaca aaattcaaca ttcacaatca gtaatttggg aatgtttggt
    10441 gttcagtcct ttggtccgat tattaaccaa cccaactcag ctatccttgg tgtcagttcg
    10501 acaatcgaga agccagttgt cgtcaatggt gaaattgtga ttcgcccaat catgagttta
    10561 ggattaacca ttgaccaccg tgtcgtagat ggtatggctg gtgctaagtt tatgaaggac
    10621 ttgaaagagt tgattgaaac gccaatctca atgttaattt aagagcaaga gtattcattt
    10681 ttaagatata gataaaggaa ggaagacatg gccttagaaa taattatgcc aaaagccggc
    10741 gtggatatga cagaaggaca aatcgtccaa tggaataaaa aagtcggaga atttgtaaaa
    10801 gaaggagaaa tccttttgga aatcatgact gataaagtca gcatggaatt ggaagccgaa
    10861 gaagatgggt acttgattgc cattctcaaa ggagatggtg aaactgtccc tgtaacggaa
    10921 gttatcggtt accttggtga agaaagggaa aacatcccaa cagctggagc agcatcgcca
    10981 gaagctagcc ctgtacctgt agcaagtacc tcaaacgatg atggtaagag cgatgatgct
    11041 tttgatatcg ttgtgattgg tggaggtcct gctggttatg ttgcagccat taaagctgcc
    11101 caactcggcg gtaaggttgc ccttgttgag aaatctgaac ttggtggaac ctgcttaaac
    11161 cgtggatgta ttccaaccaa gacctacctt catagcgctg aaattattga aaatatcggt
    11221 catgctgcaa atcgtggtat cgtgattgaa aatcctaact tcactgttga tatggaaaaa
    11281 cttttagaaa ctaaatctaa agttgttaat actcttgttg gtggtgttgc aggacttctt
    11341 cgtagctacg gagttgctgt tcataaagga attggtacaa tcactaaaga caagaacgtc
    11401 ttggtaaatg gttctgaatt gcttgaaacc aagaaaatca ttcttgctgg tggttcaaaa
    11461 gtcagcaaga tcaacgtccc tggtatggaa tctccacttg tcatgactag tgatgacatt
    11521 cttgaaatga acgaagtgcc agaaagcctt gttatcatcg gtggtggagt tgtcggtatc
    11581 gaactcggtc aggccttcat gacatttggt tcaaaagtga ctgttatcga aatgatggac
    11641 cgtatagttc cagctatgga tgtggaagtt tctaagaacc ttcgcttgat ccttgagcgt
    11701 aaaggaatga ccatcttgac tggtactaaa ctgcaagaaa tcattgagga aaatggtcaa
    11761 cttcgtatca aggttgaagg aaaagacgat atcatcgcaa gcaaagctct tctttcaatt
    11821 ggtcgtatgc cagaccttga aggtattgga gaggttgagt ttgaattgga tcgtggttgt
    11881 atcaaggtca acgaatacat ggaaacttca gttccaggca tttatgcacc aggtgacatc
    11941 aacggtacta agatgttggc tcacgcagct ttccgcatgg gtgaagtttc cgctgaaaat
    12001 gcccttaaag gaaatcatgc agttgccaaa ttgaatttga ctcctgcagc catctacact
    12061 cttcctgaag tagcagcagt aggtttgaca gaagaacaag cccgtgagaa atacgatgtt
    12121 gccatcggta agtttaactt tgctgctaac ggtcgtgcta ttgcatctga cgcagctcaa
    12181 ggtttcgtaa aagttatcgc tgataagaaa tacggagaaa tccttggtgt acacattatt
    12241 ggtcctgcag ccgcagaatt aataaacgaa gcatcaagca tcatcgaaat ggaaatcact
    12301 gttgaggaaa tgctgaagac catccacggg cacccaactt actctgaagt gatgtacgaa
    12361 gcgtttgcag atgttctagg aatggccatc cattcaccta agaaaaaata aaacgaagat
    12421 agattagact ggaaggttat ttccagtttc tatcatataa agtaaaggga tatcatgaaa
    12481 tacattatca atcattcaaa tgacactgct tttaatattg ccttggaaga atacgccttt
    12541 aaacaccttt tggatgagga tcaaatcttc ctactttgga ttaacaagcc atctatcatt
    12601 gttggtcgtc accagaacac tattgaagaa atcaaccgtg attatgttcg cgaaaatggt
    12661 attgaggtag ttcgccgtat tagtggtggt ggagctgttt accacgattt aaataacctc
    12721 aactacacga tcatctccaa agaagatgaa aataaggcat ttgacttcaa gagcttctca
    12781 actccggtta tcaatacctt ggctcaactc ggggttaaag ctgagttcac aggtcgtaat
    12841 gaccttgaga ttgatggtaa gaagttctgt ggcaatgccc aagcctatat caacggccgt
    12901 atcatgcacc acggttgctt gctctttgac gttgatttgt cagtcctcgc aaatgccctt
    12961 aaggtttcaa aagataaatt tgaatcaaaa ggtgtgaaat ccgtccgtgc ccgtgtaacc
    13021 aatattatca atgaattacc aaaaaaaatc acagtcgaaa aattccgtga tttactcttg
    13081 gaatacatga aaaaagagta cccagagatg actgaatacg tcttttcaga agaagaattg
    13141 gccgaaatca atcgcatcaa ggatactaag tttggaactt gggactggaa ctatggtaaa
    13201 tcacctgaat ttaacgtccg tcgtggaacc aaattcacta gtggtaaggt tgaagtcttt
    13261 gctaacgtta ctgaatcaaa aatccaagac atcaagattt atggtgactt ctttggtatt
    13321 gaagacgttg ctgcagtaga agctgtcctt cgtggggtga aatacgaacg cgaagatgtt
    13381 cttaaggcgc taaaaaccat tgatatcaca cgctacttcg ctggtattag ccgtgaagaa
    13441 atcgctgaag cggtagttgg ataaatcaaa aagaagttgg ccaacttctt tttttgatat
    13501 ttatttacat aatttaaaat acgtaaaatc ataaactatc cagagcattc ttttgttcat
    13561 cactaacaat atgggtatag aggtcagtga cttgtgtgct agcatgtcct agttggtgac
    13621 tgactaaaac ttgtgattta gtcgcatcat agagcctagt tgctagtgta tggcgcagtt
    13681 tatggggtgt tacacgcact ttaaaatcct ctgagtattt agcaaccatt ttctcaacgc
    13741 tagaagcatc gatacgatta ggaacacctc tgtagagagt taaaaaaagg gctgtatctg
    13801 ttttttccgt tttatagcgt tgattccgaa tggccagata attctctaaa taaggtttag
    13861 caaaagcagc gacattgact gagtcacgtt tgccaccttt tcgagtaaca tcaataacca
    13921 tcatttttag attgagatct cttagatcta gattaacagc ttcagataag cgaacaccag
    13981 atgccaagag aagggcaata atggctaaat ctcgttcttt atttttgttg aatgatgaga
    14041 gagctcgatt tgaaagctgt tgtgggtgct cttgatcgat ataagttaga aaaccttctg
    14101 tttcatcacc tagaaagagt ttttgcttga tattttcagc tctggcagca agggtttctt
    14161 tctttttctt ggttgaaact tttttcatta cattacgata gaaataaggt tccccctgat
    14221 cgttttcaac ctcctcggtt agatacttgt aaagactaga aagtgctgat aaggttcgat
    14281 tgatagttgt ctgtgaaaca ccttgttttg ttgtattagc attcagcaag ggacgttcac
    14341 gtagataaag gataaaggat tccatgtctt tcttagacat attttccaaa actgataaag
    14401 gaatatcgga tattttatca gcgtttgaaa taccagactc caaaacccag ctgaaaaatc
    14461 ggtcatattc cttaaggtat tcgtacaggg ttgtaaaact gtagggcaca gccagtttag
    14521 attggtagta ttccagaaca taccagggca tgagttgttt tagtttgtcg attcgttcca
    14581 gtaaaatctc acgtttcatg tattttctcc ataattaagt atactagtag tatagcatag
    14641 acttgtatat ttttcaagaa aattatagtt tttctgaaag atgttatagg agtttttcaa
    14701 agtgataaaa agacttagac ctacaaacaa aacagaataa ggaaaataat aaaaatctca
    14761 cccactaaac ttctgttaat actacataac tgaagagcta tataaggagc tatgagatac
    14821 aaaacacatt gtataataga ttgaaactag aatagtacat ctggacttct aaaacattgc
    14881 tagaaattga tttgattgtc ctgattgttt tatcctattt ctatttcatt tctctatata
    14941 attgctattg ttttcttttt catgatttta atcttactgt acagatttga tacagtaaac
    15001 tcctttctag ctttatactt tatcatttct aacttaatta tagtctttct aacttaatta
    15061 tagtcttatt tttaataaag ttcaattatt tatatgtaaa aatcctcatc agtaaaaaat
    15121 aaccgatctc atttctgaga atcggttttc taaataaatc aaaccgatca tttacataac
    15181 ataaattatg taaatgatca tactacaaca acaaatcttt gacttttcca atttcacttt
    15241 ttggaataac caggtgaatc atatcaccca gatacattct ggttgagccg ttaaccgttt
    15301 ggctcttgcc attatggact tgagttgtga tgaggacgtt gtgtggtaag ttgagttcat
    15361 gaacttgttt cccagcaatt ttatcagaaa ctggtatttc gataagtgta acttctcctt
    15421 cgctagatac ttcttctgga agcatttttt ccagcatggc ttcatagact ggcgtacctt
    15481 tgagcaaatc catgataata taagaaacaa gagtgacaag accaagtggc ataaggttgc
    15541 gaatatctcc taccatctca gttacgagga tcatagcggt taagggagct tttgatatgg
    15601 ctccaaaata gccactcatt cctagaatga caaatatagg gaattgctct tgactgacaa
    15661 gtccaagatt gacacagata acaccaacta aggcaccaag caaagaacca agagctaaaa
    15721 tggggaggaa aattcctcct ggcagtccac ttccatagct aatcatactc caaataaagc
    15781 ggattaaaaa gtaagctaat aaaacttgga aactaaaatt ttgttcagtt aaagaaagga
    15841 caagctgatt tccgccacca atgatttgag gtaagaagat tccgactggt atgataagga
    15901 taaaagccaa gatgggataa taagccctat ccaaatggat tttttgacca atcaagtcat
    15961 aaactcttcc aacgtttaat acagctttct catagagaaa acctgaaaat ccaaggaaaa
    16021 ttcccatgac gagatatatc caatactgat ctagggtcat gggaggaatg ttatctggca
    16081 tatccaatac tggtgtcaaa ccgaacatga gtagagacac aaagtttgct acgatgctgg
    16141 ctgctagagt tgagacccag aaaaagcgcg aaaaatggtg atagacttct tctacaacaa
    16201 agagaagtgc tgcaatagga gcattaaagg ctgcggctaa acctgctgca gctccactgg
    16261 caatcaagga acgttcctct actggactgg atttgagcca cttggcaatt cctttaccac
    16321 caactgctcc aagttgaata ctgggtccct ctcgacccag catgagtcca ctggcaatag
    16381 caagaatacc tagcacatat tttttccaaa gaatgcccca ccagttgagg gacatgaggc
    16441 ctttcagttc ggcttcgact tgaggaattc ctgagccttt aatatctttt tctgaccgtg
    16501 ttagtttggc actgagccaa cagatgagta tataaaacaa aaccagtaca aaaagattgc
    16561 gcactaagta cccttgatct tgataaactc cttgtatcag gtggaagccc ttttcaatta
    16621 agaaacgaaa ggatccgacg ataattccaa cgatgaggcc gacaatgatt cctcgaccaa
    16681 cttgggatag tatagtgctg gatgcaaagg cgaattcttt cttagaacgg actatttccg
    16741 actgttcctc catggtattt cctttcaatt aactttcttt tttacctaaa accagtgatt
    16801 taacgggttc aaagctggtt cggtgaattg gggtaactcc tagttttgtg aggccttcca
    16861 gatgtttagc tgtgccatat cctgcattag tagcgaaatc atagccaggg aactgctgat
    16921 cgtattcctt catcaattca tcacgtgtta ccttggctac tatagatgct gctgcgatag
    16981 agagggagtt ggcatctcct ttgataatgg aggtttgtga aatgggcaag tccagtttca
    17041 tggcatcaat caaaaggtgc tctggttgag ggctgagctg ggagattgct tcttgcatgg
    17101 ctagtttggt tgcttcatag atgttgactt ggtcgatgac ctgattatct atgataccaa
    17161 ttccaatcga caaggcttgg tcttgaacgg cttggaaaat ctccagatgt ttctttttag
    17221 gaattttctt gctgtcgttg agacctttaa tcttacaatt tttaggtaaa atgacggccg
    17281 cagcgactac aggaccagca agaggaccac gaccaacctc atcaatacct acaattaagg
    17341 tcaatccttg cttataaagt tctttttcat aagaaagcat ggattccaag cgcaaatttt
    17401 catctaattc agcttgaatg gctcttttac gcttgctgat ttccttttga actcctgagc
    17461 gattatcctt ttcaagctct aaaaaaatag ggctttctaa ctccttgact gtcacaagga
    17521 attctttgat ttctttaatc gttgccatcg aggtcttcca atgtatctaa ggtatagtta
    17581 ccgagtttgc catcacggac ttccttcacg aagagactgt aaaaacggtc atagtcatca
    17641 cggaaaccga gggcgcgggt catatccata ataatcacag gcgcttcttc ttcaattttc
    17701 atttgtttga agcgttcagc cagcttttct ggataatgtt ctttgaaata attgatacca
    17761 aaaatggtaa cctcatccat aggaagcaac tgatccttga tagctccagt caatgccaac
    17821 ttaagtgcaa cagtttcatc ctcaaactta ggccagagaa tccccggtgt atccaagatt
    17881 tccaggtctt tattggtttt aagccattgt tgaccttttg tgacccctgg cttgtttcca
    17941 acaacagcaa tctttttacc agccaaacgg ttcatcagag ttgatttacc agcgtttgga
    18001 atcccgataa tcatagtacg caaggtttca atctgaatcc cacgttcttt ctggcgagca
    18061 atcttatcag ccatgagctt cttggccgca tctgttacaa cttttacagt cacttgctcc
    18121 ttggagttga tagccagagt ttggattcct tgtgattcaa aatactgacg ccattccttg
    18181 gtcattgctg gatcagccaa atccgcctta tttaaaatca agagttttgg tttatcacca
    18241 acaatcttgg tcaacatagg attttgacta gatagaggta agcgtgcatc caccaaaatc
    18301 gttacaaaat caacaaattt taaattttcc tgcacctgtc gacgcgcttt agacatgtga
    18361 ccaggaaacc attgaatagt agccattgag tttttttcct ttcgtagcaa gggtttagag
    18421 atcctatttt attttactat cgtttgaaca taaagggttc tcaaatgaat gatgttcaaa
    18481 cagttttttg ttttatgaca tagaaatcta ccttatatta tatcatgcct tctttatttt
    18541 gtccgatgat agcctttcaa gtaccctata tagaaaaaag ccatcaaatg atgacttttc
    18601 agagggagca aaaaactgtt ccttctatat tctaaacatt taaatgtctt ctttaaacag
    18661 tttattaaag aaaccgtctg tgtttttaag aagggctttg aagaccttag atttaagagc
    18721 aacagtgtga gagaagtcat ctggtttctt caaatcttct tcaactgctt tatcaaataa
    18781 aggccttaac tcatcatagt tactgatatg gacgccatca atatcgatag cctcaaaacc
    18841 tttcttagct ttagcaagaa cttccccgta ccaatgtctc ttccactctt caagagtatt
    18901 aaagtttcct ttcgatactt tattaattat tagcttatcg cttagtaaac cgtggttgtt
    18961 tttatctgct tctgctttgt atttatttga tacataacta ataaagccat tttcataacc
    19021 ataatagccc cacatacgga aagcattgtg cttaaatgat aatgaaccag gagcaccttg
    19081 actcgtattc ccaccgtaaa ttcctgccat catattgaca tttacgtaag ctgagtttgg
    19141 tgtaaaatct tctggtctat aacgtccatt acctggattt ccatgttttg tcatgaagtt
    19201 attatccact aaatcattga cgctagataa agtcattacc ttttcttcat ctttcaaatc
    19261 acggatacta tcccattggt ttggacctaa aagatcatta tactgaatct tagagcgcat
    19321 ttctttgtca atcttcttga accacttact attgtctcct ttaattttag gaagtacagc
    19381 atcagcttct acatgatcaa gcaacatcat agcttcattg tagtttctca tgtagcggtc
    19441 aatgtcatca cgtgtcttta atttgtcagg atctgggttg taccattggt taccatcatt
    19501 tggacgatgg taagccatgt taattccaag tgcgccgtat tcaccattag tcgttgagct
    19561 gtcaggtgtt tgcaacatac cttgagcata ggcttcaacg tctgtacctt gacgatgctt
    19621 atgaccaccg aagtaaagca ttctatcgtt tacgtgtgtt gtttcgtgag taaaggctga
    19681 aataccaaat gggctaatca tatccgtcac cataaacaag accttgtcat cattatttgg
    19741 attagggaat attgatgcca tagcgcccat acgccagtct ctatcatggt gtcgccatgt
    19801 tggaccatat aattcacgaa ttggagctat agggttacct ttactgtcaa atcctcgacg
    19861 acctacagga gaaccattgt aggtctggtt atcccaaact ggtgtcggta ccatattttg
    19921 actcttaaga agtctatcac gaacattagg cagagctaat ctggaccaga aatctaaata
    19981 agtttgttga gcatgtgcaa cttcatcaat cttagttttg aactcattac gagcttcttc
    20041 tgtatgtttt ccatatttct caaatgaact gtacgccatc gtgttatatg ttgaaattaa
    20101 gaacatatgg gcatctttaa gcgttagaag tggtaaaatc atacgaccat gaacaccgtt
    20161 atctaaacca tcgaacacac gatgacgttt atctttcaac tctgtatttg ttgtcttagg
    20221 ttctgaaaca taaacattct tagctgagtg gataaaccaa tcattgatgt ctttatcctc
    20281 tgtaaacaga cgcatgttgt attctaagaa gttacgcaaa ttacttttac cagtattacc
    20341 tgcgatgatt tcacggtaag catcctgagt ccgatcccct ttcaagttct tttcacttga
    20401 accgatgttg atgagtctgt caataacact tggtgtttta ccataaaaat caggtttgaa
    20461 catcatgatg ttttttatac ttatgtcacc gtatttgata tcatagtaac gattgagata
    20521 agtcagagcc atcatgattt tagctttatt ggtttccact ttcttgagaa gtgattttat
    20581 cgcagcttca tcaccattca attgatggtc ttcattttcg accaaggatt tgactaactt
    20641 atctagattt tgcttcactt cggcaaagct ttcttctaag tatagatctt taatataacc
    20701 attcttacgt tcatccgtat tctctgcagg tttatcacgt ctactcatta aatctcttac
    20761 ttcttgtgaa atcaattcca cacttgataa ttgagattta acatcagcaa tcaatttatc
    20821 acgattttta acaaccatat tcggtgtata aacaacatca ccaagaccag atacactata
    20881 ttcacgcact tgttggactt tagaatcagt attcttagta acatccattt cttccttagt
    20941 accatcagca tagtgaacca taatcttatc aatatctgat aaatcggtca cgaattgacc
    21001 atccttcatc cccgtaacag aaaggacttc tttcttagct aagtttgaat cttctgcaag
    21061 tttgttacct tggttgacaa tccattcttt attataaaat ggttgaagtt tctcaatatt
    21121 acgataagct aaatcacgtt cggatttgta gtcttgagtt gacttatatt catcttctct
    21181 tctggttaaa cgatttaagc gattgaccac aggttcgttg atttcgtatt tatcagcagt
    21241 aagtccaaaa tttgcaattc tcttattagc ttcatcttga ctaatttctt taattttgtc
    21301 acgagaacgt ttgtatgaat gcttacctga actgacacct ttgacaacaa agttacgctc
    21361 taaccagcca tttgtgaaat aaccaccatc gtagtccaaa tcggaagaac caaagaactc
    21421 ttcaccattt tcaactctca tcattgaaat attatcttca agtttaccaa aaggaagatt
    21481 gctattgata aatccacctg tttcaaacaa aactttagtc ttaatggttc ctttggtgac
    21541 tgatttacga attgaaccgt atttaccaat tcccatagag tcgttaccat tatctgtttt
    21601 agcaactaaa ccacctatac gggctctgtt agcaacaata tcagcctcga catatgcttt
    21661 tgcaagatta cctctccaaa tctcaccggc aatgccagcc atatattgac ctttatcacc
    21721 aacacctgta agattaccga tgaaggctac atttgtcaac tgaccaccac tatcgatttt
    21781 attgacgata cccgcaatat tgttattagc aactacatta ccagttacct taacatcttc
    21841 aacagttgca tttttcacca tattggcaac aggtgctatt ccgtcagccc aaggcatatt
    21901 gatatccaca ttacctaaat taatattttt cacagaacca ttttcaacac ggtcaaataa
    21961 gggacgggca atgttatgaa ttgtatattg tttaccatca acacttgata gatgtccctt
    22021 aaacgtacct ggaacatatt gtttgttagg tgttggtaca ttcgttgcat tcaaatctgc
    22081 tccgatttta aatgtaccat ttttatcagc attcatggca tctactaact ctttaaagtt
    22141 ataataaacg tcatcaactt ttggaactgg tttttcaata taatgaacat acttgttacg
    22201 aaccttcgtt gggtctgtat gttgaatcaa atcttgagct tcggcagtga ttttataaag
    22261 tttcttacct tcttcagtca cttcttcaat tttatcaact gctaaacgtg ttactttatt
    22321 atcacgacta gtaattttta agtacatttt cttcacatct tcatctgaag gtttttcagt
    22381 cataaaatca ctaggtgttt cagtaccatc gtcatttacc ttcactaaat tagtagatgc
    22441 gatattttta atttcaactt ttttcaaatc caagcgaagt ggtttctctt ctaatgttgc
    22501 agtttcttct cccttgccac ggtcataggt catagtggtc tctatcttat agtccttgta
    22561 gaattttaaa tcttctacct gcttatctaa ttgctctgaa gtcaaatcta ctgttttgac
    22621 aatttcattt ccatctttaa ttgtcgcagt aattttattg attgtgactc catctgtatt
    22681 aactaaatga taagaaagag ttgcggatcg gtttaataca tttgtatttg ttctatctaa
    22741 ggtcaaaaca ggagccgttt tttctttagg tttttctttg gtacctttta caagaatttt
    22801 aggcttcatt tctttcaaaa tagttactgt ttcaactggg tcactgattt ttttgccatg
    22861 taactcttca tagctagtaa caatttgacg ttcgccatcc tcaccaactt gtctaacaga
    22921 atcatcgcct ataaatttgc taggatcaga ttcttctata gtggttttag gaatagtctc
    22981 tgtccgaata tcggtatgta aagaaggcaa ctcttctctt tctgatgcaa cttctgaaac
    23041 tgtagatatt ggttcagtat actctggaat gtcgctaaca ggtggctcaa tcaagtttcc
    23101 attttcatcc actcctgttg tacctactgg ctcagtatat tctgggatgt cgttaacagg
    23161 tggttcaatc aagtttccat tttcatctac tcctgttgtc cctactggtt cagtgtatgc
    23221 tagtttctca tgaacttcag gctcaacaag gtttgcacct attggttgcg tatagtcagg
    23281 cttctcatga acttccggtt cgcctttctc tgttacaact ttgggttgct caactggcga
    23341 aacagttgat gctggagaaa ccgacgaaac agttttatca tcaagctcca agtaaccaac
    23401 atatctgtaa ccagaaattt gcaacacacc atcacgtcca gactcatgga tgttagcatt
    23461 caaattaagt gctgaagcag ttgatagtgt aactaaacct gtcgccccta caatcaaaaa
    23521 tgtagctatt tttttatttc tattatcttt tgtgataatc acaataagac tcccgatagc
    23581 taacaaaccc aatgcagtca taatagattg agaacttcct gtgtttggta gtgcgtcttt
    23641 ttcataatcc aaagcatatg actctttgga ttcatcaggt ctaccttgtt tgagttgatc
    23701 acgttcagtt tgtgtcaaac tactataatc taagtaatga taagtcgatg caccaacaac
    23761 tgacggtgca aataaaagac tccccaagaa taccgaacca acaataccct ttattttccg
    23821 aatggaaaat ttatcttttt ttaataatga caattctaat cctccacctt cactttttat
    23881 ttcctaatag taaacactat tttttattct caaaaattga acttctaaaa tcaccggttt
    23941 ggtcaagata agctttaaag attgcttttt tcagtttaag aactctacta tttgtctcag
    24001 gatataattt ggcaacttca tcctcagcat caaattttac tgcatcttca atcatttttt
    24061 caagtctatt aatattatca atagtcactt tctgttgtcg agcccaagaa ccgtttggat
    24121 cgataaaact aactttattt aactttccaa atttatcttc tctttcttta tacatatctt
    24181 tcttgaaatc tatccaagaa ttatactggt tattaaatac ttttgaaaga acaatttcat
    24241 cagtaactaa acctatttgt ttaccatatg atgtaataac ttttccttga gctttcgctt
    24301 ctttttcata ctgattagaa atatatggaa ccataccgtc tttgtacccc ttggctgcca
    24361 ataactcaaa tgcaatacgt cgtcccatta aatctcctgg tgttcctatt ttactgctca
    24421 atgctgaata aattggtgag aatagattaa ttgtatagta gccattccgc ttataagttg
    24481 cttcatctac ataacctctt cttgttataa tatcatgttc tatcaaactg ttaaatgtag
    24541 ttaatttagt aagttcttct ggttttaatc tacgtacaac attcgttgca taaacagaat
    24601 ttccatcagg atcaggttta tattcatttt ctatttttct taataaatca tatttttgat
    24661 ttgtatctaa tttagctaca gctttacctt ctaatatttc aagcatgtat attacatcaa
    24721 acatattatg catatactta tttagatcat caacacttcc aaatcgttga gttggatctg
    24781 ctacttgaag ccttgttgat tcttctgatt gatcatattt taatatggaa ttaatagtga
    24841 ctgttggatc attaggatga tcaggagctt gtagcagtcc tttagcataa aactctggtc
    24901 caagtccatt tcgtcttcca tagcctccaa gataaatttc tctatctgag ttatgtgtca
    24961 tttcgtgtgt ataagttacc gcaccatctt tatcaagcat gcgatatgcc atataataga
    25021 atgcatcccc tgtagcatat gctccattag aattatgaac aacattattt cccgctggac
    25081 cgaagaaatg ttttatagct ggatgattag tttcaaaagt tgcttgatat gtatctttat
    25141 cttctttttc atctgtacca aatctaaatg catcatagac tagaacactt ctatataatt
    25201 tttctttatt tttatcatct agtattctat accaataatc atagtgatct ctaaatctaa
    25261 cagccgcttc tttagctttt ttctcaacat agtcatttaa atcagttcct gctggatgtt
    25321 ctttacttct atatctatca taagcaccga aacctatagt cgaaatgttg gctataataa
    25381 acaccgattt ttcaggtaat gttaatagcg gcaacaccat acttggatat ttccaagatg
    25441 ggctagttaa tctatcatat actccaattg aatatggact accagctatt gattgcttat
    25501 cacttacctc tttaatagta gatttttctt ctacaatata tgctttagtt tgttctttaa
    25561 accactcatt atttgtttta tctggtacaa atgcttttct atatttctct aatgcatcaa
    25621 ataaattatt agtcttataa tttttcgcta ataaaacatt ataagttata acattatttt
    25681 ttgctagtaa gttattatat cccgatttac ctagttcgat aacattatct aacgctgaac
    25741 tatttgattt accgaagaaa tccaagtggt acatgactaa atctttcgct tttgtatcac
    25801 gatatttgaa gtcgtaccaa cgctctaagt atgttaatcc tagaagtaaa gcttctttat
    25861 tatttttaat tttctcgatt acataattat ctattacctt attgttattt tcagctattg
    25921 cagcatctgc agtaagaagt tttgtcaaac tatctttcaa attagttttt gtggtattga
    25981 attgttcttc taaatataac tccgttaaac taacatcctt agaaacatct aatacttttc
    26041 taattgcctc tgatttatat tctacactat ttaatgctgg taatacttca tctaaaatat
    26101 tttgataatt gcgaagtaat gtattcggtg tataaattaa gtttgtaccg cctattctat
    26161 actctgctac atttgaaaaa tcagtttgat aatttacatt aagtgtctct gatgtattat
    26221 ccgcgtaata tagtaatagt ttatttatac ttgatttatt tttgttaata tcactaatta
    26281 cttctttatc tttcataggg actactgaca ataattcttt attataaaga ttactacttg
    26341 tctctactaa atttccgtac ttaacaattg tttctctatt ataaaacggt aatagttttt
    26401 caatattttt atatactttt tcttttgatg aatcagcatt ctcaagattt ttatagtcag
    26461 ttacatatct agaagagaaa ttaaaatctt ctgttttaat agtattttta gatacaaaag
    26521 tagctttttt ctcttttatt tgtgcatctt ctaatacttc tcctctttga acagtagaat
    26581 ccttagttaa gatttcatca tcttcttgta ctacgttcac aaccttatta tttttattac
    26641 tgtatgtgcc atttgctttt ataccattaa aattataacc tgcaatagca ttaccattcg
    26701 ttacattaac atcgcttagt acattagaaa gtcgaccgga tttttctaat cctccaaccg
    26761 gatcccaaag attaccaact actccaccaa cgtttgcaaa acgttgaaca ttatttagat
    26821 ttccttcagc ataactatta ctaataagcg cactattttc tactgcacca actattccac
    26881 caatagactg gtctccttgt gttgcatttg aagataaatc gattgatgcg aacgacttat
    26941 ctattaatcc cctcggacca gaaagtttgc ctactaaacc tccgatttga taactagcaa
    27001 ctgttttata tgtattagtt attctacctg tatagctact gttagaaata gtagaattat
    27061 taacttgtga tactaatcct cctatgctac gttccccagc tatcgctcca tcagcatgaa
    27121 cgttatcaat atgagtattg ttatttgctt ctttcgcaag tgttgcagta tcgtcctttg
    27181 cggaaatatt aacatctttt agtgaaagtt tttctactgt agcaccattt aattccccaa
    27241 ataatggttt tttcaaatta taaatagcat aatatttctc attatttgtt cctactaata
    27301 ttccatggaa tacattattc acataacttt cttgaccatc tggaagttca acttcacgag
    27361 catccatagt tgctcctaaa cggaatgtgc cattaggatt gctattcatt gcatctacta
    27421 aatttttaaa tgatgtatat accccagttt cactttgctg agtttttggt aaatagtatg
    27481 tataattttc tagtacatta ttattttcat gttgtatcag actatttgca cttgctacta
    27541 tcttataaac atctctatta tctttagtat cattagtaat gcttgtaact ggtagcatca
    27601 catctttaaa gttctctgat ttaactttca taaagtaatt ttctgcatta cttggtaaag
    27661 aagcaagtga agttacatgt cgatacttat taccatcttt tgaatatagt tctacagaag
    27721 atatatttct aagttctaat ttcttaataa cttctttttc atctttagcg tcatcttttt
    27781 tctcatcttt atgacgataa agttcatcgt atactggtgg aatttcatta cctgctataa
    27841 tactcttaat ccaagctaat tgtgtaggag ataaaatcaa tccgcctgag cgattttgtg
    27901 ctccattttg atgcacacca ataacttcac cattagcatt aaatattcct ccaccactca
    27961 taccaggatt tccaccttga tattgaacac cgtaggtgtt attcgcatat tcttgttcca
    28021 tttctaccgt agcatctttt aagtgcgtat atttcccagc tggatatcca tgaacattta
    28081 gtttgtcatt aagatttact ttcgaataat ttttaactaa ttctacatgt tctctgccag
    28141 ttgtttcagg tagcttaatc actgctaaat catagataaa tccttttgga taatcttttt
    28201 tattataaaa atgaatattg ttagcagtaa atgttccttt ttgtccatca ggagtcaccc
    28261 actcatatac gtttttagat ttatcacctc taaattcgcc agtagtatta tcagtagaat
    28321 tattaatgaa attatgtgct actgttaaaa gtacatttgg tgcaataaaa gtagctgatc
    28381 ctactccagt aaccttcgac gaatctgtaa taaagttcat attaattaat gcactagatt
    28441 ttttctgaat actcatttct tctggtgtat tagtattttc attatttggt ttagtatctt
    28501 ctgcactagg ttgttcgatt ttaccagtat attctttcag tgcttctact ttctctggct
    28561 cgactatcgc tccggcttgc acccctgtgt attctggtag tggtgctacc tgctctggtt
    28621 caacaatagc tccagcttgt actcctgtat attctggtag cggttctacc tgctctggat
    28681 cacccttgtc agtcatgact gcctctggta acgctggttg aacttctggt acgcctttat
    28741 cacttactac agcttctggc aactctggtt ggactgctga ctcacctttg tcggttaata
    28801 cagctgctgg tagttcaggc tgaattgcgg gttcaacaat agctccagac tgtacttcct
    28861 tatgttctgc accagtctca gcttgttcct ttataacttg agctttttta gtaccttttt
    28921 cgactattct tgaaacaggc gcagtcgttg aagttgaaat aatttctcgt gaaacttctt
    28981 cattgtttac agaaaatatt ctgacgattt caactttctt acctaatgca ccttcttgtt
    29041 ttactcttac agttccttct gctaaatcag gattttcttg aatttcttct tgaaaatcta
    29101 tttttgtttc tatagtttcg tcacgatata agagttccgg tttgttcaac tgacctgata
    29161 gcacttcatc ctgtggattt aatgtattta tcccagtctt ttcctttgga gaaatcttct
    29221 cctctttctt cgtttctaga ttcttatgtt cggctaattg ttcttgagaa tctgaagatt
    29281 gtttctcttc ttttcttgga ttgattaatt cagtagagaa aggtttttca actacttgaa
    29341 cttctgtcgg cttagttgaa gaaacaggtg tttgttcctg aatagcttgt actgttgatg
    29401 gatggtctac aaaatttggt gtaacattat aatccacctt ttgttgtttt gtaggagtgg
    29461 ctgctgattt ttcttgatta cttacttcaa aatcagaagt cgtttttccc tctttgatat
    29521 atccaatata agtgtaacct gaaatctctt taggaagagg taatttttct ccagaggtca
    29581 attcatagtc cgtattgtaa tttagcaaaa gatgattttc taaagcatgg actgaaacta
    29641 agacaccatt tcccattccc gcaaccaata ctaaatgtaa taccgtttta ttcttaacct
    29701 ttttcttgga aacagcaaaa attaaaattc ccagagcagc taagctagca ccagcaacta
    29761 gggcttgcct ctcattcttg cttccagtat ttggtaattc cgccagttga ttttgagaat
    29821 ttaacttata aacaagataa taagtttcat catcattctc cacgtatgtc ggaatatcat
    29881 agacaagctg cttcttttct tctgatgata gctctgaatc tgccacatat ttatagtgaa
    29941 ctcccgcagt ttcttgagca tccacagatg aactagctaa tacagacata aaaaataaac
    30001 ttgaaatcgt tgcagataca agtcctactg ataattttct aaatgaaaaa cgctcttgtt
    30061 tttcaccaaa atacttttcc attattcctc cttgaaataa aatttatata tgttacaaag
    30121 acctttatta tattagtgta ttatctatta tctatagaaa aggcagtata ccttaattat
    30181 actcttaatt tacaaaaaag tcttaaaatt gagatgcgct ttcatacttt gttttatatt
    30241 atttgggggt acaataacac ctaccatgaa atttacacgg taggtgttac tcatatcact
    30301 aatcgttcta aaaatggttt gaggcagttg aggagaattc cttctatcca gcttccttgt
    30361 gctgatgagc gatggtcttc ctgcaggctt ttttttagaa aatctcggac ttgttctggt
    30421 gcgatttcaa attcaaaggc tttcatttta tagaaaaagt cgatgagatg atcggacagg
    30481 tattcagttg aaaagggtac ttcaccactt tttcgatatt ctaataagag cctagaaaat
    30541 cgagcttttt cttcaggaag ctcacgaaaa taggaattga ggagccaagt ctgcttctgt
    30601 tttctttcaa ttggatcctg actggcaatt cgttggtctt tttccagctc tttttggtat
    30661 tgtttggcct tgatagcccg ttctgctcta tttttaccaa aaagaatttt ttcccacttg
    30721 cgttcttctt gagtcagggt ctctgtaaag ccaaagtaat cttgataagc acgctctgcg
    30781 ggtcccatgg ctagaaccag attgtctgca tattgcttgg cgattttatc cctcttcttg
    30841 cgttctttct ctgcctggat acggagttct tgttcgtagt caattttctc cttgcctagc
    30901 ttgacaaggt agagttggtc atccgatttc ccaagtaaaa agggtttgat acacttttca
    30961 aggacttctt ccatccgagc ctttttcttt ggttccgcct tggtccaact tcctccctga
    31021 aagacttcta ggaaaagctg gtagtctctc tcaggcgcaa attgattgcc acgattgggt
    31081 ttgaaaacac ctttttccca gagccatttt agaagtcgct cgtcaaagtc acttttattg
    31141 accttgattt tttccttttt ctgagctttt ctggttagat tttcaacctt tctgagcagt
    31201 ttttcttcct cttccaattg ctggtcaagg gacaatcgat gaaaatgacg aacacagtcg
    31261 ctaccaattg gaaagaggcg ttggcctgtg acaccgttaa agagttcata agcgtatttg
    31321 atggcatttc cacagacaca attgctacgg ccgataccgt taaaaatcaa ggaaacttca
    31381 ttccattcct tggtagcttg ttcccaagta tccgctttcg aagcctgtaa aactgcatcg
    31441 tgcagggatt ttctaactgg aagtgtcatg aggtctcctt tctaatactc aataaaaatc
    31501 aaagagcaaa ctagaaagct agccgcaatc agctcaaaac ac
//
LOCUS       DAVMMR010000023        31381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMMR010000023 DAVMMR010000000
VERSION     DAVMMR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.023"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            184..1056
                     /gene="rsmA"
                     /locus_tag="VYS74_000999"
     CDS             184..1056
                     /gene="rsmA"
                     /locus_tag="VYS74_000999"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV3789055.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            1058..1936
                     /gene="rsgA"
                     /locus_tag="VYS74_001000"
     CDS             1058..1936
                     /gene="rsgA"
                     /locus_tag="VYS74_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV3789056.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            1947..2603
                     /locus_tag="VYS74_001001"
     CDS             1947..2603
                     /locus_tag="VYS74_001001"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV3789057.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            2566..3228
                     /locus_tag="VYS74_001002"
     CDS             2566..3228
                     /locus_tag="VYS74_001002"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV3789058.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            3230..4486
                     /locus_tag="VYS74_001003"
     CDS             3230..4486
                     /locus_tag="VYS74_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV3789059.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            4476..5417
                     /locus_tag="VYS74_001004"
     CDS             4476..5417
                     /locus_tag="VYS74_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV3789060.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            5684..6511
                     /gene="purR"
                     /locus_tag="VYS74_001005"
     CDS             5684..6511
                     /gene="purR"
                     /locus_tag="VYS74_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV3789061.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            6578..7828
                     /locus_tag="VYS74_001006"
     CDS             6578..7828
                     /locus_tag="VYS74_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV3789062.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            7954..8748
                     /gene="pflA"
                     /locus_tag="VYS74_001007"
     CDS             7954..8748
                     /gene="pflA"
                     /locus_tag="VYS74_001007"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV3789063.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(8823..9749)
                     /gene="yidC"
                     /locus_tag="VYS74_001008"
     CDS             complement(8823..9749)
                     /gene="yidC"
                     /locus_tag="VYS74_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3789064.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(9827..10105)
                     /locus_tag="VYS74_001009"
     CDS             complement(9827..10105)
                     /locus_tag="VYS74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV3789065.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            10139..10879
                     /locus_tag="VYS74_001010"
     CDS             10139..10879
                     /locus_tag="VYS74_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV3789066.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            10935..11618
                     /locus_tag="VYS74_001011"
     CDS             10935..11618
                     /locus_tag="VYS74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV3789067.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            11730..12722
                     /locus_tag="VYS74_001012"
     CDS             11730..12722
                     /locus_tag="VYS74_001012"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV3789068.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            12787..13326
                     /gene="rsmD"
                     /locus_tag="VYS74_001013"
     CDS             12787..13326
                     /gene="rsmD"
                     /locus_tag="VYS74_001013"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV3789069.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            13316..13804
                     /gene="coaD"
                     /locus_tag="VYS74_001014"
     CDS             13316..13804
                     /gene="coaD"
                     /locus_tag="VYS74_001014"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV3789070.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            13788..14825
                     /locus_tag="VYS74_001015"
     CDS             13788..14825
                     /locus_tag="VYS74_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV3789071.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            14883..15113
                     /locus_tag="VYS74_001016"
     CDS             14883..15113
                     /locus_tag="VYS74_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV3789072.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            15137..16420
                     /gene="murA"
                     /locus_tag="VYS74_001017"
     CDS             15137..16420
                     /gene="murA"
                     /locus_tag="VYS74_001017"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3789073.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            16413..16601
                     /locus_tag="VYS74_001018"
     CDS             16413..16601
                     /locus_tag="VYS74_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3789074.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            16640..17464
                     /gene="endA"
                     /locus_tag="VYS74_001019"
     CDS             16640..17464
                     /gene="endA"
                     /locus_tag="VYS74_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV3789075.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            17755..19086
                     /locus_tag="VYS74_001020"
     CDS             17755..19086
                     /locus_tag="VYS74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV3789076.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(19284..19354)
                     /locus_tag="VYS74_001021"
     tRNA            complement(19284..19354)
                     /locus_tag="VYS74_001021"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19320..19322),aa:Cys,seq:gca)
     gene            19770..23381
                     /gene="rpoB"
                     /locus_tag="VYS74_001022"
     CDS             19770..23381
                     /gene="rpoB"
                     /locus_tag="VYS74_001022"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3789077.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            23414..27091
                     /gene="rpoC"
                     /locus_tag="VYS74_001023"
     CDS             23414..27091
                     /gene="rpoC"
                     /locus_tag="VYS74_001023"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV3789078.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            27202..27615
                     /gene="ndk"
                     /locus_tag="VYS74_001024"
     CDS             27202..27615
                     /gene="ndk"
                     /locus_tag="VYS74_001024"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV3789079.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(28544..29176)
                     /locus_tag="VYS74_001025"
     CDS             complement(28544..29176)
                     /locus_tag="VYS74_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789080.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(29178..31193)
                     /locus_tag="VYS74_001026"
     CDS             complement(29178..31193)
                     /locus_tag="VYS74_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3789081.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT         9126 a         6240 c         7134 g         8881 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtatgaac gtatgtttcg agagaagcac taagtccctc
       61 tttaaagatt tccaagacta tctttcttca tgaggaaaga tagttttttg gtatgatttt
      121 cattcccaaa atacaagggg aatgtgttac aatagtagta acagataata gaaaagagaa
      181 tagatgagaa ttgcagatta tagcgtgacc aaggcagtgt tggagcgtca cggttttacc
      241 tttaaaaagt cctttgggca aaatttcttg acggatacca atatccttca aaaaattgtg
      301 gatacggctg aaattgatga tcaggtcaat gtcatcgaaa tcgggccagg tattggtgcc
      361 ttgacagaat ttttggctga gcgtgcagcc caagtcatgg cttttgagat tgaccaccgt
      421 ttgatgccaa ttttggcaga tactctgcgt gattttgata atgtgaccgt agttaacgaa
      481 gatattctca aggttgattt ggcgcaacat atccaaaatt ttaaaaatcc taacctgcca
      541 atcaaggtag tggctaattt gccttactac atcacgacgc ctattctcat gcacttgatt
      601 gagagtggca ttcctttttg tgagtttgtg gtcatgatgc agaaagaagt agcggaccgc
      661 atttcagccc agcctaacac caaggcttac ggtagcttgt ctatcgccgt gcagtattac
      721 atgacagcca aggttgcctt tatcgtgcct cgtacggtct ttgtgccagc gccaaatgtg
      781 gattcagcca tcttgaaaat ggtgcgtcgt ccagagccag ccgtagcagt agaagatgag
      841 aactttttct ttaaggtttc caaggctagt tttacccatc gccgcaagac cttgtggaat
      901 aacttgacag gttactttgg taagactgaa gaggtcaagg acaagctgac caaggctttg
      961 gaccaggcag gcttgtcacc aagtgtgcgt ggggaagctc tcagcttggc agaatttgcc
     1021 ggtctagcag acgcacttaa agggcaagga ctctaagatg cagggacaaa tcattaaagc
     1081 cttggcaggt ttctactatg tggagagtga tggccaggtt tatcaaacac gcgcgcgtgg
     1141 gaatttccgt aaaaaaggcc atacccctta tgttggggac tgggtagatt tctctgccga
     1201 ggaaaattca gaaggctata tcctcaaaat tcacgaacgg aaaaacagtc tggttcgtcc
     1261 gcctattgtc aatatcgatc aagctgtagt aatcatgtcc gtcaaggaac ctgattttaa
     1321 cagcaatttg ctggatcgtt tcttggttct tttggagcac aagggcatcc atcccattgt
     1381 ctatatttcc aaaatggatt tgttggaaga taggggagaa ctggattttt accagcagac
     1441 ctatggtgac atcggctatg actttgtgac cagtaaagag gaactcctgt ctttgttaac
     1501 aggcaaggtt acggtcttta tggggcagac aggtgttggg aagtcaactc ttctcaataa
     1561 aatcgcacca gacctcaatc ttgaaacggg agaaatttca gacagtctag gtcgcggtcg
     1621 ccataccact cgagctgtta gtttttacaa tctcaacggg ggtaaaatcg cagatacacc
     1681 aggcttttca tctctggact atgaagtatc aagggctgaa gacctcaatc aggcctttcc
     1741 agagattgct actgttagcc gagattgtaa gttccgtact tgtacccata cccatgagcc
     1801 gtcttgtgcc gtcaaaccag ctgttgaaga gggtgttatt gcaaccttcc gttttgacaa
     1861 ttacctgcaa ttccttagtg aaattgaaaa tcgtagagaa acctataaaa aagtcagcaa
     1921 aaaaattcca aaataaggag aaacctatgt ctcaatacaa gattgctccg tcaattctgg
     1981 cagcagatta tgccaacttt gaacgtgaaa tcaaacgtct agaagcaact ggggcagaat
     2041 atgcccatat cgatatcatg gacagtcatt ttgtaccgca aatcagtttt ggtgcaggtg
     2101 tggtcgagag ccttcgtcct catagtaaga tggttttcga ttgccacttg atggtgtcaa
     2161 accctgagca tcatctggaa gattttgcgc gtgcaggtgc agacatcatc agtatccatg
     2221 tagaagcaac gcctcatatt catggcgccc tccaaaaaat tcgttcactc ggagttaagc
     2281 cttcagtcgt tatcaatcct ggcacatcag ttgaagccat caagcacgtc cttcatctag
     2341 ttgaccaagt tttagtcatg acggttaatc caggttttgg tgggcaagcc tttctgccag
     2401 aaaccatgga taaggttcgt gagttggttg ctcttcgtga ggaaaaaggt ttgaactttg
     2461 aaatcgaagt ggatggtggg attgatgacc aaactattgc ccaagccaaa gaagctggtg
     2521 cgaccgtttt tgtagcaggt tcctatgtct ttaagggaga tgtcaatgag cgagtacaaa
     2581 ctctcagaaa acaactggac tagggttgcc gtttttgcag gcggagaccg cggtcattat
     2641 cggacagatt ttgatgcttt tgttggggtg gatcgaggct cgctctgggt cttggaagaa
     2701 gacttgcttc ttgctctagc agttggggat tttgattctg tgaccgaaga agagcgacag
     2761 gtgattcaaa aacgtgccca gcattttgtc caagcccgac cagaaaaaga tgataccgat
     2821 ttggaattgg ctctcttaac catctttgag aaaaatcctc aggctcaggt tactattttc
     2881 ggtgccttgg gtggccgtat tgaccatatg ttggccaatg tctttctgcc tagcaatcct
     2941 aagttggcac cctatatgca tcaaatagaa attgaggatg ggcaaaactt gattacttat
     3001 tgtccagaag ggaccagtca gctagaacct cgttcagact acgactatct agcctttatg
     3061 ccagttcggg atagccagct gactattctt ggagccaagt atgagttgac agaggaaaat
     3121 tttttcttta aaaaagtgta cgcttctaac gaatatatag atagggaagt gtcggtaact
     3181 tgcccagatg gttatgtggt cgtactgcat agcaaggaca ggaggtagga tggaaagttt
     3241 acttattcta ttattaattg ccaacctagc tggtctcttt ctgatttggc aaaggcagga
     3301 taggcaggag aaacacttaa gtaagagctt ggaggatcag gcagatcatt tgtcagacca
     3361 gttggattac cgctttgacc aagccagaca agccagccag ttagaccaaa aagatttgga
     3421 agtggttgtc agcgaccgtt tgcaagaagt gcggattgaa ttgcaccaag gtctgaccca
     3481 agtccgtcaa gaaatgacag ataatctcct ccaaactaga gacaagacag accaacgtct
     3541 ccaagccttg caggaatcaa atgagcaacg tttggaacaa atgcgccaga cggtcgagga
     3601 aaaactagaa aagaccttgc agacacgctt acaggcttcc tttgagacag tttctaaaca
     3661 actggagtct gtcaatcgtg gccttggaga aatgcagaca gttgcccgtg atgtcggagc
     3721 tcttaacaag gttctctctg gaaccaagac gcgagggatt ctgggagaat tgcaactggg
     3781 gcaaattatt gaagacatca tgacacctgc ccagtacgaa cgagaatacg caacggttga
     3841 aaactctagt gaacgagtgg agtatgccat caagttaccc ggacaaggcg accaagaata
     3901 cgtctatctg ccaattgact ctaagtttcc actggcagat tattaccgct tggaagaagc
     3961 ctatgagaca ggtgacaagg atgagattga acgctgtcgt aagtcactcc tagcaagcgt
     4021 caagcgcttt gccaaggata tcaagagcaa gtacatagca ccgcctcgga cgaccaattt
     4081 tggagttttg tttgttccga cagaaggtct ttattcagag attgtgcgca atccggtctt
     4141 ctttgatgat ttgagacggg aagaacagat tatcgttgca ggaccaagta ccctatcagc
     4201 ccttcttaac tccctatcag ttggtttcaa gacccttaat atccaaaaga gtgccgacca
     4261 tatcagcaag actcttgcca gtgtcaagac cgagtttggc aagtttggtg gtattctggt
     4321 caaggcacaa aaacatctcc aacatgcctc tggcaatatt gatgaattat taaaccgtcg
     4381 taccatagct atcgagcgga cgctccgtca cattgagttg tcagaaggcg agcctgcgct
     4441 tgatctactc cattttcaag aaaatgagga agaatatgaa gattagtcac atgaaaaaag
     4501 atgagctatt tgaaggcttt tacctaatca aatcagctga cctgaggcaa actcgagctg
     4561 ggaaaaacta cctagccttt accttccaag atgatagtgg cgagattgat gggaagctct
     4621 gggatgccca acctcataac attgaggcct ttaccgcagg taaggttgtc cacatgaaag
     4681 gacgccgaga agtttataac aatacccctc aagtcaatca aattactctc cgcctgcctc
     4741 aagctggtga acccaatgac ccagccgatt tcaaggtcaa gtcaccagtt gatgtcaagg
     4801 aaattcgtga ctacatgtcg caaatgattt tcaaaattga aaatcctgtc tggcaacgga
     4861 ttgtccgaaa tctctacacc aagtatgata aggaattcta ctcctatcca gctgccaaga
     4921 ccaaccacca tgcctttgaa acgggcttgg cctatcatac ggcgaccatg gtgcgtttgg
     4981 cagacgctat tagcgaagtt tatcctcagc tcaataagag cctgctctat gcggggatta
     5041 tgttgcatga cttagctaag gtcatcgagt tgacggggcc agaccagaca gagtacacag
     5101 tgcgaggtaa tcttcttgga catatcgctc tcattgatag cgaaattacc aagacagtta
     5161 tggaactcgg catcgatgat accaaggaag aagtcgtttt gcttcgtcat gtcatcctca
     5221 gtcaccacgg cttgcttgag tatggaagcc cagtccgtcc acgcattatg gaagcagaga
     5281 ttatccatat gattgacaat ctggatgcaa gcatgatgat gatgtcaaca gctcttgctt
     5341 tggtggataa aggagagatg accaataaaa tcttcgctat ggataatcgt tccttctata
     5401 aaccagattt agattaataa tttaagaaaa atgagcattt tttaggataa gaatgttcgt
     5461 ttttttatgt gaatatggta taataagtaa aagacaaaaa tgaatactct tcgaaaatct
     5521 cttcaaacta gggtagtatc gccttgtcgt atgtatatat gcaggtatat tacagggttt
     5581 gtcagttcta ttgacaatct caaaacagtg ttttgaacca ccagcgacca gctttctagt
     5641 ttgctttttg attttttgaa taaaaatgga ataggaaata gaaatgaaat taagaagaag
     5701 tgatcggatg gttgtcattt ccaactattt gattaataat ccttataaac taactagtct
     5761 caatactttt gctgaaaagt atgagtctgc taaatcatcc atctcagaag atatcgtcat
     5821 tatcaaacgc gcctttgagg aaattgaaat cggtcatatc cagacagtga ctggggctgg
     5881 cggaggtgtc atcttcacac cgtctatttc gagtcaggat gctaaggaaa tggttgaaga
     5941 cttgcgtacc aagttgtcag aaagtgaccg tatcttgcca ggtggttata tctatctgtc
     6001 tgatttgctt agcacaccag ccatcttgaa aaatattggt cgtattattg ccaaaagctt
     6061 tatggaccaa aaaattgacg cggttatgac cgtagcaact aagggtgtgc cacttgcaaa
     6121 tgcagttgcc aatgtcctca atgtctcttt tgtcattgtg cgccgtgacc tgaaaattac
     6181 cgaaggttca actgttagcg tcaactatgt ttcaggttca agtggtgacc gtatcgagaa
     6241 aatgttcctt tcaaaacgta gtcttaaggc aggtagccgt gtcttgattg tggatgactt
     6301 cttgaaaggt ggcggaacgg tcaacggtat gattagtctc ttgcgcgagt tcgactcaga
     6361 actggcaggt gtagcggtct ttgcggacaa tgcccaagaa gaacgtgaaa agcagtttga
     6421 ctacaagtca ctcttgaagg taactaatat tgatgtcaag aaccaagcca tcgatgttga
     6481 ggttggcaat atctttgacg aagataaata agagatagaa ctaaaggttg gaacgattgt
     6541 cccagccttt ctttgcaaac agaatagaag gaagcttatg aaaacaccat ttatcaatag
     6601 agaagagtta gaagcgattg ttgccgagtt cccgactccc tttcacttgt atgatgagaa
     6661 ggggattcgt gagaaggcaa gagccgtcaa ccaagctttt tcgtggaaca agggctttaa
     6721 ggaatatttt gcagttaagg ctactccaac tccagctatt ttgaaaattc tccaagaaga
     6781 aggttgtggt gtggactgct ctagttatgt agagcttttg atgagccata aactggactt
     6841 tctgggttct gagattatgt tctcttccaa caacacgcca gacaaggaat acgcctatgc
     6901 acgtgaattg ggtgcgacca ttaacttgga tgcctttgaa gatattgaac atctggagag
     6961 agtagcaggc attccagaaa tcatctcttg tcgttataat cctggaggcg tttttgaact
     7021 ggggacagac attatggaca atcctgggga ggctaagttt ggcatgacca aggaccagct
     7081 ctttgaagcc tttgctatct tgaaggaaaa aggagccaag acttttggga ttcactcctt
     7141 cctagcgtcc aataccgtga cccatctcta ttatccagag ttggctcgtc agctctttga
     7201 actggctgtt gaaatcaagg aaaagttggg catttggcta gactttatca atctttctgg
     7261 cggtattggt gttaattatc gtccagacca ggagccgaac gatatcgcct tgattggtga
     7321 gggagttcgt aaggtgtatg aagaagttct tacgtcagca ggtcttggtc aggtcaagat
     7381 tttcaccgaa ttgggtcgtt ttatgctggc acctcacggt gctctagtca caagagtgac
     7441 tcataagaag gaaacctacc gtacctatct aggtgtggat gcctcagcag tcaacctcat
     7501 gcgtccagct atgtacggag cttaccatca tattagcaac gtgacccatc cagatggacc
     7561 agctgaagcg gtagatgtgg ttggttcact ctgtgaaaac aatgataaat ttgcagtcaa
     7621 tcgcgaactg cctcatacag aaatcggtga tttgctggta attcatgata caggtgccca
     7681 cggattttca atgggctacc agtataatgc caaattacgt tctgcggaaa tcctctatac
     7741 cgaagaaggt aaagcccgtc aaatccgccg tgcagagcgc cctgaggact attttgcaac
     7801 cttatatggc ttcgattttg aagaataatc tgataataga ttgaaaatga aattgaaaaa
     7861 cagattgctt tctaaaaaat aggcaaaaat cttgtttttc cttcaagtcg tgatataata
     7921 aaactataaa acgttttcaa ggaaggtaac gatatgtctg aagaaacaat tgattatgga
     7981 caagtgacag gaatggtgca ttcgacagaa agctttgggt cagtagatgg gcctggtatt
     8041 cgctttattg tctttttgca gggctgtcac atgcgttgcc agtattgcca caacccagac
     8101 acttgggcta tggagtccaa taagtcacgt gaacggacgg tagatgatgt cttgacagag
     8161 gccttgcgct accgtggttt ctggggaaat aagggtggga ttacagtcag tggaggagaa
     8221 gctctcttgc agattgattt cctgattgct ctcttcacca aggctaagga acaaggaatc
     8281 cactgtacct tggatacctg tgctcttcct ttccgtaata aaccacgtta ccttgagaag
     8341 tttgacaaac tcatggctgt cactgacttg gttcttttgg atatcaagga aatcaacgaa
     8401 gaacagcaca agattgtcac tagacaaacc aataaaaata tcttggcttg tgcccagtat
     8461 ctatcagata ttggaaaacc tgtctggatt cgccacgtgc tagttccagg attgacagac
     8521 agagatgatg acttgattga acttggtaag ttcgtcaaga ccctcaaaaa tgttgataag
     8581 tttgaaattc taccttatca caccatgggt gagttcaagt ggcgtgaact tggaattcca
     8641 tattccctcg aaggagtcaa accaccaaca gcagatcgcg tcaagaacgc taaaaaactc
     8701 atggataccg aaagttatca agattatatg aaacgtgtac atggatagaa aagaagcctg
     8761 atggaaacat cgggcttttg atttgcaaaa agacttagca aatcagctaa gcctttttct
     8821 tcttatctcg aacgttgttt tccagcgttg cgatttttgt gttttttctt gcttgtgata
     8881 gcagttggtt gttcaggggt aacgtctttt cgtccacttg gtttagagaa agcacttgct
     8941 tttggtgggt tcttggctag ttcttcacgg acttttttgc gaagttttgg acgaacgata
     9001 tagttgacga taaactgttg gagaatcatc atgaaaccac cgacaaccca gtaaagtgtg
     9061 acactagctg gtgagaagag ggagaagacg acgatcatga gtgggctcat gtaaatcatt
     9121 ttcttgattt gttctctttg catttcatct tctactccgt gaagtgaaag gagcgattga
     9181 agatagtaaa ggacaccagc acaggcaacc aaaatcatac ttggagaacc tagaggaatg
     9241 cctaggtagc ttgcttgagc aaccccttca gtatgttggg cagcaaagta gatagcagag
     9301 aagaaaggca tttgaaggag gatagggaaa catcctacac cgccaaacat gctgataccg
     9361 tgctcttttt gagcagcaaa gagagcttgt tgggcttcga gtttttcttc ttgagtagtc
     9421 gcttctttga gacgcgtttg gtgtggctca aggacgtgct tgagggcgtt catcttttca
     9481 gagtgaagcg ttgccttcca tgattggtag ataccaagtg gcaagataat caagcgcacg
     9541 ataatggtta cgatgatgat agcgacacca aagcctagac ctttatcagt agcgaagtac
     9601 ttgatagctt cagccatagg ggctccgatg gtattccaaa taaatcctgt tggctgacct
     9661 gtggttttat cgacattgac acaaccagtc aagacaagca acatagccgc tcccatagct
     9721 gagagtgcaa aacgtttaat agatttcact gtttttattc cttctttaaa aattatacct
     9781 ttctattcta ctgttttttt acaaaatata caatagttct ggagacctaa tttgcgattt
     9841 taaagtcaga gtagggagga aagttgctta gtttgacatc taagtagctt acttttgaaa
     9901 aaggtgtcgg tccttttcgg atttcttgga taaattttgc catgatagca gatgagtctg
     9961 cttgggctaa gatttccact gtgccatcgt cgttattcca tactcgacct gtgatgccac
    10021 caatttcaag tgccaagctg taaacacccc aacgaaagcc gactccctgt accctacctt
    10081 gggcaatcat tctaaccttt tgcataccaa acccctttga ttgtgttata atatttctat
    10141 gactattata acctcaaaag ccaattctgt ggtaaaaaat gccaagaaat tacgccaaaa
    10201 aaaataccgc aagtctgcct acttgattga aggctggcac ttgtttgaag aagctgtcca
    10261 agctggagtg acgattgaga aaatctttgc cctagaaaat tatcgagatc agttagctac
    10321 tttttcgcaa actgtctggg tgtcagagga tattttgcta gatttggcgg attctcagac
    10381 tccacaggga attgttgccg tggttcaaaa agaagaagta ggacaagctg atttgagtca
    10441 gggcaagttc ttgtttttgg aagatgtgca agatcctggt aatgtaggaa ctatcattcg
    10501 aactgcggat gcagcaggtt ttacaggagt gattgtttca gataagtcgg cagacatcta
    10561 cagtctcaaa accctacgtt ccatgcaagg tagtcatttc catctgccca tttaccggat
    10621 gactagtcaa gcgcttcttg acgaaactaa aaaggtagct atcccagtgc tagcaacaac
    10681 cctatctaaa gattctgttg attacagaga actgccttct atagaaaatt ttgtactagt
    10741 tatgggaaat gagggtcaag gaattagtcc ccttatggct gaaagtgcag accagttggt
    10801 ccatattagc atgaaggggc aggccgagag tttgaatgtt gcggttgcag ccggtatttt
    10861 aatcttccat ttaagctaat tttaactttt tttgttataa tcaaggaaag atgttcacag
    10921 aaaaggagaa atggatgaat cacactatta tacatgaccg tgcaggtctc aatcaatttt
    10981 acgctaaggt ttatgccttt gttggtctgg gaatcggact atctgctttg gtatcaggcc
    11041 ttatgttgac ggtctttcag tctcagttgg tttacttttt gatgcagggg cgtctctggt
    11101 tgaccattgc tacttttgcg gagttggctt tggttttcgt tgccagtagc atggcttcaa
    11161 gaaatagtcc agctgctctt ccagtatttt tactttactc cgttttaaac ggctttaccc
    11221 ttagttttgt ggtggctttc tatactccgg gtacagtttt atctgccttt gtatcgagcg
    11281 cccttctctt ctttgtcatg gcggcagttg gtatttttac caagaaagac ttgagtggga
    11341 ttggtcgagc aatgatggca gctctcattg gtttactgat tgctatggta gtcaatattt
    11401 tcttggctag tggcttcttt gattatatga ttagcgtagc catggttttg gttttttcag
    11461 ggttgattgc ttgggataac caaaagattc gcctcgccta tgaacaatca caaggtcgtg
    11521 tagcgacagg ctgggtggtg tcaatggctc tcagcatcta tcttgatttt atcaatcttt
    11581 tcctaagtat tctacgtatc tttggtcgca atgactaaaa ggtctatgaa atcagtgttt
    11641 tgagtgacac tgattttttt taaattttct cttttctttt cttggaaata ggtgtataat
    11701 acgtttatta aatttttgag gagttgtcta tgaagaaaag ttttatccat caacaagaag
    11761 aaatttcctt tgtcaaaaac acttttaccc agtatttgaa agataagcta gaagttgtcg
    11821 aagttcaagg tcctatcttg agtaaggtcg gtgacggaat gcaggacaac ctgtctggtg
    11881 tggaaaatcc agtatcggtc aaggttctcc aaatccctga tgctacttat gaagtggtgc
    11941 actcacttgc taaatggaaa cgccacacct tggctcgttt tggctttggt gaaggagagg
    12001 gtctctttgt ccacatgaaa gcccttcgtc cagatgagga ttccttggat gcaacccact
    12061 ctgtttatgt tgaccagtgg gactgggaga aggttatccc aaatggtaag cgtaacatcg
    12121 tttatctaaa agaaacagtt gagaagattt ataaggctat tcgcctgact gagctagctg
    12181 ttgaagcccg ctatgacatc gagtctatct tgccaaaaca aatcaccttt atccatacag
    12241 aagaattggt agaacgctac ccagacttga cacctaaaga acgtgaaaat gcgatttgta
    12301 aagaatttgg agccgtcttt ttgattggta tcggtggcga gttgccagat ggtaaaccgc
    12361 acgatggacg tgcaccagac tatgatgact ggacaagcga gtctgagaat ggctacaagg
    12421 gtctaaatgg tgatattctt gtctggaatg agtctttagg tggagccttt gagttgtctt
    12481 ctatgggaat tcgtgtagat gaagaaactc ttagacgtca ggtggaaatt acaggtgatg
    12541 aagaccgctt ggaattggaa tggcacaagt ctttgttgaa tggcctattc ccattgacaa
    12601 tcggtggagg aattggacaa tctcgtatgg ccatgttcct acttcgcaag agacacatcg
    12661 gagaagtgca aacaagtgtt tggcctcaag aagtccgcga tacttacgaa aatattttgt
    12721 agagaatcga accgcaaggt tcggttttct ttctcttttt gtctataatt tggtataata
    12781 aacagtatga aaatcgtatc aggaatctat gggggacgtc ccctcaagac actagaaggc
    12841 aagacaacaa gacctacttc ggataaggtt aggggagcca tttttaacat gattggtccc
    12901 tactttgaag ggggacgagt cttggacctt tatgcaggta gtggtggttt atctatcgaa
    12961 gcagtatcgc gtggcatgtc cagtgctgtt ttggtggagc gagaccgtaa ggctcagacc
    13021 atcgtggctg aaaatatcca gatgaccaag gaagttggaa aatttcaact cctcaagatg
    13081 gatgcagaga gggcattgga acaggtatct ggggaatttg acctcgtttt cttagaccct
    13141 ccctatgcca aggaacaaat cgtagcagat attgaaaaaa tggctaagag agagcttttt
    13201 tctgaagatg ttatggttgt gtgcgagacg gataaggccg ttgaacttcc agaagagatt
    13261 gcctgtctgg gtatctggaa ggaaaagatt tatggaatta gtaaggtgac agtctatgtc
    13321 agataagatt ggcttattca caggctcatt tgatccgatg acaaatgggc atctggatat
    13381 cattgaacgg gcgagcagac tttttgataa gctttatgtg ggtatttttt ttaatcccca
    13441 caaacaagga tttctccctc ttgaaaatcg taaacggggg ctagaaaagg ctttgggaca
    13501 tttggaaaat gttgaagtcg tggcttctca tgatgaattg gtggtcgatg ttgcaaaaag
    13561 actgggggct actttcctag tacgggggtt gaggaatgcg gcggatttgc aatatgaagc
    13621 tagttttgat tactacaatc atcagctgtc ttctgatata gagactattt atttacatag
    13681 ccggcctgaa catctctata tcagttcatc aggtgttcga gagcttttga agtttggtca
    13741 ggatattgcc tgccatgttc ccgagagtat tttggaggaa ataagaaatg aaaaaaaaga
    13801 ttagatggcc cttatacgtc attgcggcct tgattgtgac tttcttggca tttgtagtgc
    13861 ccttgcctta ttatatagag gttccaggtg gttcggaaga tattcgccaa gtccttaaag
    13921 taaatgacac agaagataag gaagctggtg cctatcaatt cgttacggtt ggtgttcaac
    13981 atgccacttt agctcatatg atttatgctt ggttgacacc ttttacagat attcgtagtg
    14041 ctcaggagac tacaggtggt tcttccgatg ttgaatttat gcgaatcaat caattctaca
    14101 tgcaaacatc gcaaaatatg gccaagtatc aaggactaaa aacagctggt aaggatatcg
    14161 aactcaagta ctttggagtt tatgttttga atgtgacgga taattcaacc tttaaaggga
    14221 ttctcaatat ctctgataca gtcacagcag tcaatgatca gacctttgat agttccaaag
    14281 acttgattga ttacgtcagt tctcaaaaat taggggattc cgtcaaggtc acctatgaag
    14341 aggatgggca aaccaagtct gcagaaggaa agattatcac cttggaaaat ggcaaaaatg
    14401 gaattggaat cggcttgatt gaccgtacag aggtaatcag caatgtccca attagctttt
    14461 caacagctgg tattggcggc ccaagtgctg gtctcatgtt tagtctagct atctatactc
    14521 aaatagctca cccagatctt cgtaatggtc gtattgttgc cggtacaggt accattgacc
    14581 gcgatggtaa tgtgggagac attggaggta ttgataagaa ggttgtagct tcggctaggg
    14641 caggtgctgc tattttcttt gctcctgata accctgttag cgaagaagaa caaaaggcgc
    14701 atctggacgc gaaaaacaac taccaaacag ccctagaagc ggctaaaaca atcaagacgg
    14761 atatgaaaat cgtgcccgtt aaaaccctac aagatgcgat tgattacttg aaaaacaatc
    14821 cctaattttt gagtgaagtt tccaaactag cgcacaaaca gagaagatgg tataatagtc
    14881 aaatggttca attattattc actctaagta gtcatatgct ctttatttat gtgagttttt
    14941 accttttaaa ggatcttgtt agatgggaga aggttttaaa agtgacagat gataatacaa
    15001 gaaaagttcg tttattagta gcctttttta gcattgtcat aggctacatc ctgagttctt
    15061 tctttattag cctgtatcat ttgtggcaag aagcgcttag aggattatta taaaatcaag
    15121 agtaaaggaa acgagtatgg ataaaattgt ggttcaaggt ggcgataatc gtctggtagg
    15181 aagcgtgacg atcgagggag caaaaaatgc agtcttaccc ttgttggcag cgactattct
    15241 agcaagtgaa ggaaagaccg tcttgcagaa tgttccgatt ttgtcggatg tctttattat
    15301 gaatcaggta gttggtggtt tgaatgccaa ggttgacttt gatgaggaag ctcatcttgt
    15361 caaggtggat gctactggcg acatcactga ggaagcccct tacaagtatg tcagcaagat
    15421 gcgcgcctcc atcgttgtat tagggccaat ccttgcccgt gtgggtcatg ccaaggtatc
    15481 catgccaggt ggttgtacga ttggtagccg tcctattgat cttcatttga aaggtctgga
    15541 agctatgggg gttaagatta gtcagacagc tggttacatc gaagccaagg cagaacgctt
    15601 gcatggcgct catatctata tggactttcc aagtgttggt gcaacgcaga acttgatgat
    15661 ggcagcgact ctggctgatg gggtgacagt gattgagaat gctgcgcgtg agcctgagat
    15721 tgttgactta gccattctcc ttaatgaaat gggagccaag gtcaaaggtg ctggtacaga
    15781 gactataacc attactggtg ttgagaaact tcatggtacg actcacaatg tagtccaaga
    15841 ccgtatcgaa gcaggaacct ttatggtagc tgctgccatg actggtggtg atgtcttgat
    15901 tcgagacgct gtctgggagc acaaccgtcc cttgattgcc aagttacttg aaatgggtgt
    15961 tgaagtaatt gaagaagacg aaggaattcg tgttcgttct caactagaaa atctaaaagc
    16021 tgttcatgtg aaaaccttgc cccacccagg atttccaaca gatatgcagg ctcaatttac
    16081 agccttgatg acagttgcaa aaggcgaatc aaccatggtg gagacagttt tcgaaaatcg
    16141 tttccaacac ctagaagaga tgcgccgcat gggcttgcat tctgagatta tccgtgatac
    16201 agctcgtatt gttggtggac agcctttgca gggagcagaa gttctttcaa ctgaccttcg
    16261 tgccagtgca gccttgattt tgacaggttt ggtagcacag ggagaaactg tggtcggtaa
    16321 attggttcac ttggatagag gttactacgg tttccatgag aagttggcgc agctaggtgc
    16381 taagattcag cggattgagg caagcgatga agatgaataa gaaatcaagc tacgtaatca
    16441 atcgtttact tttagtcatc atagtactga ttttaggtac tctggctcta ggaatcggtc
    16501 taatggtagg ttatggaatc ttgggcaagg gtcaagatcc atgggctatc ctgtctccag
    16561 caaaatggca ggaattgatt cataaattta caggaaatta ggctggagaa ccagcctttt
    16621 tctaaagata aggagaaata tgaacaaaaa aacaagacag acactaatcg gactgctagt
    16681 gttattgctt ttgtctacag ggagctatta tatcaagcag atgcagtcgg cacctaatag
    16741 tcccaaaacc aatcttagtc agaaaaaaca agcgtctgaa gctcctagtc aagcattggc
    16801 agagagtgtc ttaacagacg cagtcaagag tcaaataaag gggagtctgg agtggaatgg
    16861 ctcaggtgct tttatcgtca atggtaataa aacaaatcta gatgccaagg tttcaagtaa
    16921 gccctacgct gacaataaaa caaagacagt gggcaaggaa actgttccaa ccgtagctaa
    16981 tgccctcttg tctaaggcca ctcgtcagta caagaatcgt aaagaaactg ggaatggttc
    17041 aacttcttgg actcctccag gttggcatca ggtcaagaat ctaaagggct cttataccca
    17101 tgcagtcgat agaggtcatt tgttaggcta tgccttaatc ggtggtttgg atggttttga
    17161 tgcctcaaca agcaatccta aaaacattgc tgttcagaca gcctgggcaa atcaggcaca
    17221 agccgagtat tcgactggtc aaaactacta tgaaagcaag gtgcgtaaag ccttggacca
    17281 aaacaagcgt gtccgttacc gtgtaaccct ttactacgct tcaaacgagg atttagttcc
    17341 ctcagcttca cagattgaag ccaagtcttc ggatggagaa ttggaattca atgttctagt
    17401 tcccaatgtt caaaagggac ttcaactgga ttaccgaact ggagaagtaa ctgtaactca
    17461 gtaaaagata agcctaaact cctatgtcac ttatggatgt aggagttctt tttactagtt
    17521 taagcagggc tagaataggt gctaagaaaa aatagtaact tctaaaacta acttccagtt
    17581 tcccacaacc tagcttttag tatgagaaaa acgcgtgaaa tcagtggaaa tccgtcttag
    17641 actaacttcc agttttggga gagagatgga agttacgcta agaaaaaaat aatatgtatt
    17701 ggattaaatt ttgtgatata ataaaggata agatactatt ttaggagaag aactatggaa
    17761 gacccaagta gtcagaattt gttgctacaa tttgttttat tgtttatctt gacggtatta
    17821 aatgcctttt tctcagccac agaaatggcc atggtttctc ttaatcgtgc ccgagttgaa
    17881 caaaaggcgg aagaaggaga cagacgctac atccgcctgc tgaaggtact agaaaatcct
    17941 aaccactttt tatcaaccat tcaggtagga atcaccctga ttacaatctt atcaggggcg
    18001 agtttaactg atactctagg acgtgtgatt gcctcttggc ttgggaatgg cgaaacagca
    18061 caagccgtgg caacttttct atccttggca tttttgacct atatttccat cgtttttggg
    18121 gaattatatc ctaagagaat cgctcttaat ctaaaggatg ccttggcaat tcgtacagcg
    18181 ccgattatca ttgggatcgg aaaactagtc agtccttttg tttggctttt agctgcttcg
    18241 accaatttct tgagccattt gactcctatg tcattcgatg atgcggatga aaagatgacc
    18301 cgtgatgaaa ttgcctatat gctgacaaat agtgaagaaa cattggatgc tgatgagatt
    18361 gagatgctac aaggtgtctt ttcgctcgat gaactgatgg cacgagaggt tatggtgcct
    18421 cgaacggatg cctttatggt ggatattcag gatgatagtc aagccattat ccaaagtatt
    18481 ttaaaacaaa attattctcg tatcccggtt tatgatgggg ataaggacaa tgtaattgga
    18541 atcattcaca ccaagagtct ccttaaggca ggctttgtgg acggttttga caatattgtt
    18601 tggaagagaa ttttacaaga tccacttttt gtacctgaaa ctatttttgt ggatgacttg
    18661 ctaaaagaac tgcgaaatac ccaaagacaa atggcaatct tgctggatga atacggtggt
    18721 atggctggtt tggtgacttt ggaagacctc ttagaggaaa ttgttggtga aattgatgat
    18781 gaaacggaca aagcagcaat agatgttcat cagattggcg aggataccta cattgttcaa
    18841 ggaactatga ccctcaatga tttcaataat tactttgatg ttgaactgga aagtgatgac
    18901 gttgatacca tcgctggtta ttatttgaca ggagtgggaa ctattccaac gactgagaaa
    18961 ctcagctatg aattggtcag ccaaaacaaa cagtttatct taaccaatga taaagtgaag
    19021 aatggacgtg ttaccaaggt aaaagtccaa atcacagaag tcgaaataga agaagaaaca
    19081 gaataaacta gaggcttttg tcaccaggcg tgacgaagct tttttcaatt gatttcaaaa
    19141 gggaattatg ccatggaaag aactctgcca caaactcgct ccaaaagttt ttaaagttat
    19201 acgtatttat tcgagggaaa ataagaaaag cccttcaaat tgggctttct gttcatttga
    19261 aacctgataa atcaagttat agaaggcggt agacggattt gaaccgacga tcaagctttt
    19321 gcagagccgt gccttaccac ttggctatac cgccttaacg tttattatta taccttgaaa
    19381 atgatttcca gtcaatagtt ttttcagttt tttgtaccat agcgtgattc gaatgtgatg
    19441 ccaagttttc tatgggaaaa aatttaccaa aaacctactg aaaccccttg aaaaatctag
    19501 ctaaataggg tataatatgg gtaatcattt gtcgtaggtt ttgtctgaaa tattgtccag
    19561 acaaggctca cagcagttaa atcttctgaa aaagtcagat ttagctgctc tttttgtgct
    19621 ttttttcaag attatgagca tttgtaacag aggcttaaag attctgaaaa ttcgtcaaga
    19681 ggacacggtg ataaggggtt tacaaccata tgacgattag aaaagcctga ttgacaaggc
    19741 ttggaactta tttacaaagg agaatcatct tggcaggaca tgacgttcaa tacgggaaac
    19801 atcgtacccg tcgtagtttt tcaagaatca aagaagttct tgacttacca aatttgattg
    19861 aaattcaaac tgactcattc aaagctttcc tagaccacgg tcttaaggaa gtgtttgaag
    19921 atgtattgcc aatttcaaac ttcacagaca caatggagtt ggaatttgtt ggatatgaaa
    19981 tcaaggaacc aaaatacacg ctagaagaag ctcgtatcca cgatgctagc tactcagcac
    20041 caatttttgt aaccttccgt ttgatcaata aagaaacagg cgaaatcaag acccaagaag
    20101 ttttctttgg tgatttccca atcatgacag aaatgggtac tttcatcatc aatggtggtg
    20161 aacgtattat cgtttctcag ttggtccgct caccaggtgt ttactttaac gacaaagtag
    20221 acaaaaatgg taaggtgggc tatggttcaa ctgttatccc taaccgtgga gcttggttgg
    20281 aacttgaaag cgactcaaaa gatatcacct acactcgtat cgaccgtact cgtaagattc
    20341 catttacaac cttggttcgt gctcttggtt tctcaggtga tgatgaaatc tttgatatct
    20401 ttggtgacag cgaattggtt cgcaacactg ttgaaaaaga tatccacaag aatccaatgg
    20461 actctcgtac agacgaagcc ttgaaagaaa tttacgaacg ccttcgtcca ggtgagccta
    20521 agacggctga aagctcacgt agcttgcttg tagctcgctt ctttgaccca cgtcgttatg
    20581 acttggcagc agttggtcgt tacaaaatca ataaaaaact caatgttaaa acacgtttgc
    20641 tcaaccaaac cattgcagag ccattggtag accctgaaac tggagaaatc ttggtagaag
    20701 ctggtacgat tatgactcgt agcgtgattg aaagcattga aagccatttg gatggcgact
    20761 tgaacaagat tgtctacatc ccaaacgatg cagccgttgt gactgagcct gttgttcttc
    20821 aaaaattcaa ggttattgct ccaactgatc cagatcgcgt cgtaacgatc attggtaatg
    20881 ctaacccaga tgacaaggtt cgtacggtga ctcctgcaga tatccttgct gagatgagct
    20941 acttcctcaa cttggctgaa ggacttggcc gtgtagatga tatcgaccac cttggaaatc
    21001 gtcgtatccg tgcggttggt gaattgcttg ccaaccaagt acgtttggga ctttctcgta
    21061 tggaacgtaa tgtccgtgaa cgtatgtctg ttcaggacaa tgaagtcttg acaccacaac
    21121 aaattatcaa tattcgtcct gtaacagctg cagttaaaga attctttggt tcatcacagt
    21181 tgtcacagtt catggaccaa cacaacccgc tttctgagtt gtctcacaaa cgccgtttgt
    21241 cagccttagg acctggtggt ttgactcgtg accgtgccgg atatgaagtg cgtgacgtgc
    21301 actacactca ctatggtcgt atgtgtccaa tcgagacacc tgaaggacct aacatcggtt
    21361 tgatcaataa cttgtcatct tacggacact tgaacaaata tggttttgtt caaacaccat
    21421 accgtaaggt tgaccgtgaa acaggtgttg tcacgaacga aattgtttgg ttgacagctg
    21481 atgaagaaga tgaatatact gtagctcagg ctaactctcg tctgaatgaa gatggaacct
    21541 ttgctgagaa gattgtcatg ggacgtcacc aaggggtcaa ccaagagtat ccagctaata
    21601 ttgttgacta catggacgtt tcaccaaaac aggtagttgc cgttgcgaca gcatgtattc
    21661 ctttcttgga aaacgatgac tccaaccgtg ccctcatggg agccaatatg caacgtcagg
    21721 ctgtgccatt gattaatcct caggcacctt acgttggtac tggtatggaa taccaagcag
    21781 cccacgattc tggtgcggct gtgattgctc agtatgatgg taaagttact tacgcagatg
    21841 ctgacaaggt agaagttcgt cgtgaagatg gttcattgga tgtttaccac atccaaaaat
    21901 tccgtcgttc aaactcaggt actgcttaca accaacgcac tctcgtaaaa gttggtgatg
    21961 tcgttgaaaa aggcgatttc atcgctgacg gaccttctat ggaaaatgga gaaatggcgc
    22021 ttggacaaaa cccaatcgtt gcctacatga cttgggaagg ctacaacttc gaggatgccg
    22081 ttatcatgag cgaacgcttg gtgaaggacg atgtctacac atctgttcac cttgaagaat
    22141 acgaatcaga aacgcgcgat acaaagcttg ggcctgaaga aatcactcgc gaaattccaa
    22201 acgttggtga agatgccctc aaagaccttg acgaaatggg gattatccgt attggtgctg
    22261 aggttaaaga aggtgatatt cttgtaggga aagtaacacc taagggtgag aaagatcttt
    22321 cagctgaaga acgtctcttg cacgctatct ttggagacaa gtctcgtgaa gtgcgtgata
    22381 cttctcttcg tgtaccacac ggtgccgatg gtgtcgttcg tgatgttaag atctttacac
    22441 gtgtaaatgg agatgagttg caatcaggtg ttaacatgtt ggttcgtgtt tacatcgctc
    22501 aaaaacgtaa gattaaggtc ggagataaaa tggccggacg tcacggaaac aaaggggttg
    22561 tctctcgtat cgttcctgta gaagacatgc cttaccttcc agacggaact ccagtcgaca
    22621 tcatgttgaa cccacttggg gtgccatcac gtatgaatat cggtcaggtt atggagcttc
    22681 accttggtat ggcagctcgt actcttggta ttcacattgc gacaccagtc tttgatggag
    22741 caagttctga agatctttgg tcaactgtta aagaagcagg tatggatagc gatgccaaaa
    22801 cgatccttta cgatggacgt actggtgaac catttgataa ccgtgtttct gttggagtca
    22861 tgtacatgat caaactccac cacatggttg acgataaatt gcacgcgcgt tcagtcggac
    22921 cttactcaac tgttacccaa caaccactcg gaggtaaagc tcagtttggt ggacaacgtt
    22981 tcggtgagat ggaggtttgg gctcttgaag cctacggtgc gtcaaatgtc cttcaagaaa
    23041 tcttgactta caagtctgac gatatcaacg gacgtttgaa agcctatgaa gctattacaa
    23101 aaggcaaacc aattccaaaa ccaggtgttc cagaatcctt ccgagttctt gttaaagaat
    23161 tgcaatctct tggtcttgac atgcgtgtcc tagacgaaga tgaccaagaa gtggaacttc
    23221 gcgacttgga tgaaggaatg gacgaagatg tcatccacgt agatgacctt gaaaaagccc
    23281 gcgaaaaagc agcccaagag gctaaagcag cctttgaagc tgaagaagct gagaaagcaa
    23341 caaaagcgga agcaacagaa gaagctgctg aacaagaata agcagttcac ttagaataga
    23401 aagggaagaa atagtggttg atgtaaatcg ttttaaaagt atgcaaatca ccctagcttc
    23461 tccaagtaaa gtccgttcat ggtcttatgg agaagtcaaa aaacctgaaa caatcaatta
    23521 tcgtaccttg aaaccagaac gtgaaggact ctttgatgaa gtgatctttg gtcctacaaa
    23581 agactgggaa tgtgcttgtg gtaagtacaa acgcattcgt tacagaggaa ttgtttgtga
    23641 ccgctgtggg gttgaagtaa cgcgtacgaa agttcgtcgt gagcgtatgg gacatatcga
    23701 attgaaagct cctgtatctc acatctggta cttcaagggg attccaagcc gtatgggctt
    23761 gacccttgat atgagccctc gtgccctcga ggaagttatc tactttgcgg cttatgtggt
    23821 gattgatcct aaggatacac cacttgagca caagtctatc atgacagagc gcgaataccg
    23881 agagcgcttg cgcgaatatg gttatggttc atttgttgcc aagatgggtg cggaagccat
    23941 ccaagacctt ttgaagcaag tagatcttga aaaagaaatt gctgaactca aagaagaatt
    24001 gaaaacagct actggacaaa aacgtgtcaa agccatccgt cgtttggatg ttttggatgc
    24061 cttttacaag tctggaaaca aacctgaatg gatgattctt aacatccttc cggttatccc
    24121 accagatctt cgtccaatgt tgcagttgga tggtggccgt tttgcctcat ctgacttgaa
    24181 tgacctttac cgccgtgtta tcaatcgtaa caaccgtttg gctcgtttgc ttgagttaaa
    24241 tgcaccaggt atcatcgttc aaaatgagaa acgtatgctt caagaagcag ttgacgcttt
    24301 gattgacaat ggtcgtcgtg gtcgtccaat cacaggacca ggtagccgtc cattgaaatc
    24361 attgagccac atgcttaaag gtaaacaagg acgtttccgt caaaacttgc tcggtaaacg
    24421 tgttgacttc tcaggacgtt cagttatcgc cgttggtcca actcttaaga tgtaccaatg
    24481 tggtgtgcca cgtgaaatgg cgattgaact ctttaaacca tttgtcatgc gtgaaatcgt
    24541 tgcccgtgat atcgtgcaaa acgtcaaagc agctaaacgc ttggtggaac gcggagatga
    24601 gcgtatctgg gatatccttg aagaagtgat taaagaacac ccagtgcttt tgaaccgcgc
    24661 accgaccctt caccgtttgg gtatccaagc cttcgagcca gtcttgattg atggtaaggc
    24721 tcttcgcttg cacccacttg tctgtgaagc ctacaatgct gactttgacg gggaccaaat
    24781 ggccatccac gtaccacttt cagaagaagc acaagcagaa gctcgtatcc tcatgctagc
    24841 tgctgagcac atcttgaacc cgaaagatgg gaaaccggta gttactccat ctcaggacat
    24901 ggttttgggt aactactact tgaccatgga agaagctggt cgcgaaggtg aaggaatggt
    24961 cttcaaagac cgtgacgaag cggttatggc ttaccgcaat ggttatgttc acctccactc
    25021 acgtgttggt atcgcaacag acagcctcaa caagccttgg acagaagagc aaagacataa
    25081 ggtcttgctt acaacagttg gtaaaattct cttcaacgat atcatgccag aggggctacc
    25141 atacttgcaa gaaccaaaca atgccaactt gacagaaggt gttccagcta aatacttctt
    25201 gccacttggt ggagatatca aggaagctat cagcaatctt gagctcaacc ctccattcaa
    25261 gaagaaaaac ctcggaaata tcatcgctga aatcttcaaa cgtttccgta cgacagaaac
    25321 ttctgcccta cttgaccgca tgaagaacct cggttaccac cactcaactc ttgcaggatt
    25381 gacagtgggt attgccgata tcccagtcgt tgatgacaag gctgaaatca ttgaagaatc
    25441 acacaaacgt gtcgaacaaa tcaccaaaca attccgtcgt ggtatgatca cagacgacga
    25501 gcgttacaat gctgttacag ctgaatggcg tgctgcccgt gaaaaacttg agaaacgctt
    25561 gattgctaat caagatccta agaacccaat cgttatgatg atggactctg gagcccgtgg
    25621 taacatctca aacttctcac agcttgccgg tatgcgtggt ctgatggctg ctccgaatgg
    25681 acgtatcatg gaattgccaa tcctttcaaa cttccgcgaa ggtttgtcgg tattggaaat
    25741 gttcttctca actcacggtg ctcgtaaagg tatgaccgat acggccctta agacagccga
    25801 ctcaggttac ttgactcgtc gtttggtcga cgttgcccaa gacgttatca tccgtgagga
    25861 cgactgtgga actgaccgtg gtctcttgat ccgttctatc gcagaaggaa aagagatgat
    25921 cgagtctctc gaagagcgtc tcaacggtcg ttacactaag aaaactgtta aacatccaga
    25981 aactggtgca gtgattattg gtccaaatga attgattaca gaagacaagg cgcgtgaaat
    26041 tgtcaatgct ggtgtggaag aagtgactat ccgttctgta tttacatgta acactcgtca
    26101 cggtgtctgc cgtcactgtt acggtatcaa cttggcgact ggtgatgcgg ttgaagttgg
    26161 tgaagcagtt ggtacaatcg ctgcccaatc tatcggggaa cctggtacac agcttacaat
    26221 gcgtaccttc cacacaggtg gggttgcctc aaataccgat atcactcagg gtcttcctcg
    26281 tgtccaagaa atctttgaag cccgcaatcc taaaggggaa gcggttatca cagaggttaa
    26341 aggacaagtt actgctatcg aagaggatgc atcaactcgt accaagaaag tctttgttaa
    26401 gggtgaaact ggcgaaggtg aatatgtcgt tccatttaca gctcgtatgc gtgtcgaagt
    26461 tgggggccaa gtagcgcgtg gtgctgctct gacagaaggt tctatccaac caaaacgtct
    26521 ccttgcagtt cgtgatgtct tgtcagttga aacgtacctt ctcggtgaag tacaaaaagt
    26581 ttaccgtagc caaggggtag aaatcggtga caaacacatc gaggtaatgg ttcgtcaaat
    26641 gatccgtaaa gtccgtgtca tggatccagg tgatacagat cttctcatgg gtaccctcat
    26701 ggatatcaat gactttacag atgccaacaa agatgtcctt atcgcaggtg gagttccagc
    26761 gacaggtcgc ccagtcctta tgggaattac caaagcctca cttgaaacaa acagtttctt
    26821 gtcagcggct tccttccagg aaacaactcg tgtccttact gacgcagcta tccgtggtaa
    26881 gaaagaccat ctccttggac ttaaagaaaa tgttatcatc ggtaagatca tcccagctgg
    26941 tactggtatg gcccgttacc gtaaccttga accacatgct gtcaacgaag aagaatacct
    27001 taatcctcca gtagaggaag aaggaaatga agaaacaaca gaagtagttg tggatactgc
    27061 cgttgaaact gtggaagaaa cagtagaata agagagaatg agagaaccct cgggttctct
    27121 cattctctct tctgaaaact ttctccattt ttccacgaaa tgtggtaaaa taaaagaaag
    27181 aagctgacaa aggagacggg tatggaacaa acattcttta tcatcaaacc agatggtgta
    27241 aaaagagggc tagtgggtga agtgttaaag cgcatcgaac aacgtggatt tacaatcgaa
    27301 aaattggagt ttcgttcaca ggtttcagaa gagttgattg accagcacta tcaggacttg
    27361 gttggtcaga gtttttaccc accgattcgt gaattcatga cttcaggtcc agttcttgtg
    27421 ggtgtcattt ctggtcccaa agtaatcgaa acttggcgga ccatgatggg tgcaactcgt
    27481 ccagaagaag ctttaccagg cactattcga ggtgattttg caaaagctgc tggagaaaat
    27541 gagattatcc aaaatgttgt acatggttca gattcagaag agtcagctaa gcgagaaatt
    27601 gctctttggt tttaagagtg gattggctca atcaattggt taaaagctca tttgaataga
    27661 aagtatagtc aattagttta agacatgacg catgatatca aactttttag tttttgatat
    27721 ggtgcgtttt ttgattaagt gactattagt ttgtctcgct ccgttaggtg cgagacaaaa
    27781 aaaacacccg ctatgcgggt gcgcgtcgaa ggttatacca aaaaaactcc aaacgcgata
    27841 caataaaggt gttcaatcca attgtaaagc gaaaggagaa aaatatggca caaaaggttc
    27901 ttttatcctg ttcttgtttc aattgactat atttctatac tttttcttcc tttctctgtg
    27961 aaaatcgcaa gtttttccca ccttttctca atactgaaac aacatctttc cagaacttca
    28021 ataatttctt tttgtgttaa atcattattt aactaaatct ttggaggcaa tacgttttca
    28081 aagctacttt tttaccatag gttttgagcc ttcaacacag attactaagc caaaaatgct
    28141 ctaaattttt gaaattctaa caaattccac tcaactttat catcatcttt gactttcaca
    28201 atatcttcct caaaataaca tggaataaag atagttttct tttaaaacgg ttgtaggagg
    28261 aaaatctctc ctacactatt attgattctg agagaaaatt gcaaaatatc atctaccaaa
    28321 ctttcaatta ttaagatttt ttgcagttta tctgaattag aatcgcaagg cttctcaata
    28381 atataattgt cgaatttttt cattctaact tcctctccat tatttcttcc tttatcaaga
    28441 aaaagttacc tcaatattaa taaagtacaa ttctaaatta actccaattc ttgattcatg
    28501 ataatatttc agtaactcat cgtgcaatta aaaacaagga tttttatttc agatgatcca
    28561 tcgtgaacac ttcatcagcc atctcccaaa ttgccggatt atgtgttgcg ataatgatta
    28621 gcctattatc atctcgaaga gatagcaaaa tctccataat caactgagag gttgctgggt
    28681 ctattgaagc tgttggctca tctgccagaa taaagggtgg attctttaag ataatttttg
    28741 ccaaggcaac ccgttgcgat tctccgcccg ataactcaaa gatgcgctta tctaggtcaa
    28801 gataagccag gccgacctgt tctaaagcct gcttctgcct caaccgctgt tccgaccgac
    28861 tcaacttttg accaatgaga cctagcttaa ggttttcttc aatactttgg ttttcaatta
    28921 agccaaagtt ctggaagagg tagcccaatt cgtgacggaa aaaatcactc gatttataat
    28981 tggccaagtc tttacctcgg taaaaaatcg tcccatcata aggttctaat ttcccaatca
    29041 tgttcatcaa ggttgttttt ccgctaccac ttgaaccaat taaggcatag acttttccag
    29101 cctcaaatgt cattgaaaga ttcgaaaata actctcgttc tccaaaagat ttactcacct
    29161 gtttaagttc aatcatatta acctcccttc aaaacaagaa tggacatctt gttttctttc
    29221 tgcatttgga catgtaactg taaaagagat agaccagtaa agagtaacaa gactaagaaa
    29281 gcaactccta tctctacctg aagaaataca ctcgcaacaa atcccagtaa aaacacaccc
    29341 agttgagcaa agagataagt gcgatggatt tctaagaacc tgagacctgc aatgcgtttg
    29401 ataaaaatgg cacgtctaaa ttcttcaaag tagagcctat tcatagtgtt aaacaacaag
    29461 attgaagttg caatcccaag cacagctcct gctagcatta cccaacgttc cctctggata
    29521 ttatccaata atgtgatgta gttgtggtaa cctgtttgca tttctgagac ccaattttca
    29581 atgccttgtc tctggataag ctcctgggca tcagacaatt gattaaagag aacgtagttc
    29641 tgtactgcgt ctatccaaaa caaaatggac tgtggaccag ttgattgggg tgttataaca
    29701 atgatgattg gatctttcaa aaactgctgg taagaaatag gggtcgtatt atacacaaaa
    29761 cgatcctgac ctgattctaa ataacctacc gtagcagtca tttgctgtcg ttcatcttga
    29821 ctagacatgc gactggttaa gtcgtcttca aaaacagatt tataatgttc ttcctctgaa
    29881 cggaggtgtt caggcagcaa taagacaaac tcccctacat taaggtgatt catcttttgc
    29941 tcaatagttg tatctacagg aatgttttga cgctccaagt attgcggtgt gacgataagg
    30001 acattgccat tcgggttgta atcgtgccat tctgtagagg ttatcaagtt tttggaggaa
    30061 gccatgccat tttgcaaagt acggtcaatt aactggtgtc tagataagaa agccttttgt
    30121 tctgaaacag ccagatccat caattgatac caagttctga gtttcctttg agcttgttca
    30181 tcaaaaccag gactcgttcc ttcacggctg attgataggg taatcagttg cctttcctga
    30241 ctccaagcct cttgtccaat ccgatgctgt tgccaggctt gggaatactt taagctactc
    30301 cctattccca gcgttacaat aataatcgct aatagttgac cgattaaaat caaactaatg
    30361 attcctctga caggcacttg cccttttata atctgcatca ggtgtatctt ttttatccca
    30421 actgcgaaga gttgagcgaa aaacagggag atcgacaaca agatgagatt ataactgagc
    30481 aagcctgctc ctatggtcat tagggattgc gttggaagcg acagaatttt ttgcataaga
    30541 atggcgagca cgccagctaa actgaaacca acagctatcc ctttcaaatc ctcaccaact
    30601 ggtctaagga aaatggacca ccgtttctct cctgaaatga ggcgaatgcc cgacgaccgt
    30661 aattggctaa tttgactaat taaagtcagt gctacaaagg ttaagatgaa aatcaataga
    30721 ctgattaact gaaatccgtt actaaagata gccattaacg tagatagttt agatggaatt
    30781 gtcaggttca tgttagtcaa gccaagttga cttagctcct cccttagcaa gtgaatatct
    30841 aggtgtccat cgaatacaaa atagtttgtt tcaacactac tactttgagc atcttctagt
    30901 tttttttcct gaagcccatc aggcaacttt ccgtccccaa aggtcgtata agaaaaaact
    30961 gtagtacctt ctgagttaga atcttggtgt tgaatcgcaa taaagctatc tgtttcttct
    31021 gctagttttt ccaagcgtgt agcaacatgc tgaaaagttg tttcagggga agaatcacgt
    31081 acaacaacgt tggggtaata atgggagaca tatgtatcag tccaaatggt aaatacccaa
    31141 acaaagaaca aactagcaag caggttggat atgagtataa ataatttttt catagtgcat
    31201 tccttattct aaaacagagg gcagaaatac ccctgccctc tataaactaa caagcttact
    31261 aaactattga taaaagaatc ttcttcaatt attctttacc atcccgcatc aaatgtaact
    31321 aattttttaa ttccacgttt ttttctcatg caatgaaacc cctttctttt tccttgtttt
    31381 t
//
LOCUS       DAVMMR010000024        30375 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMMR010000024 DAVMMR010000000
VERSION     DAVMMR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.024"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..316)
                     /locus_tag="VYS74_001027"
     CDS             complement(<1..316)
                     /locus_tag="VYS74_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEV3789082.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            complement(372..797)
                     /locus_tag="VYS74_001028"
     CDS             complement(372..797)
                     /locus_tag="VYS74_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3789083.1"
                     /translation="MPKRYLLDGKTPRISVTNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYIEDLKLKCNIANYNI"
     gene            complement(1185..1979)
                     /locus_tag="VYS74_001029"
     CDS             complement(1185..1979)
                     /locus_tag="VYS74_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789084.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(1979..2986)
                     /locus_tag="VYS74_001030"
     CDS             complement(1979..2986)
                     /locus_tag="VYS74_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3789085.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(2986..3993)
                     /locus_tag="VYS74_001031"
     CDS             complement(2986..3993)
                     /locus_tag="VYS74_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3789086.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(3983..5008)
                     /locus_tag="VYS74_001032"
     CDS             complement(3983..5008)
                     /locus_tag="VYS74_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV3789087.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            complement(5401..5619)
                     /locus_tag="VYS74_001033"
     CDS             complement(5401..5619)
                     /locus_tag="VYS74_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789088.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(5910..6143)
                     /locus_tag="VYS74_001034"
     CDS             complement(5910..6143)
                     /locus_tag="VYS74_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEV3789089.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCN"
     gene            complement(6357..7988)
                     /gene="rlmD"
                     /locus_tag="VYS74_001035"
     CDS             complement(6357..7988)
                     /gene="rlmD"
                     /locus_tag="VYS74_001035"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3789090.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRNNSHNLMPLEDFF
                     IQDPVIDQVVVVLRDLFRRFDLKPYDEKEHSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(8062..9036)
                     /locus_tag="VYS74_001036"
     CDS             complement(8062..9036)
                     /locus_tag="VYS74_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV3789091.1"
                     /translation="MRKKLFLTGAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(9110..9724)
                     /gene="pmp23"
                     /locus_tag="VYS74_001037"
     CDS             complement(9110..9724)
                     /gene="pmp23"
                     /locus_tag="VYS74_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV3789092.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(9724..10698)
                     /locus_tag="VYS74_001038"
     CDS             complement(9724..10698)
                     /locus_tag="VYS74_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV3789093.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(10706..11962)
                     /locus_tag="VYS74_001039"
     CDS             complement(10706..11962)
                     /locus_tag="VYS74_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV3789094.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(12024..12455)
                     /locus_tag="VYS74_001040"
     CDS             complement(12024..12455)
                     /locus_tag="VYS74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789095.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(12457..13059)
                     /locus_tag="VYS74_001041"
     CDS             complement(12457..13059)
                     /locus_tag="VYS74_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV3789096.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(13043..13882)
                     /gene="prmC"
                     /locus_tag="VYS74_001042"
     CDS             complement(13043..13882)
                     /gene="prmC"
                     /locus_tag="VYS74_001042"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV3789097.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(13882..14961)
                     /gene="prfA"
                     /locus_tag="VYS74_001043"
     CDS             complement(13882..14961)
                     /gene="prfA"
                     /locus_tag="VYS74_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV3789098.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(14958..15548)
                     /locus_tag="VYS74_001044"
     CDS             complement(14958..15548)
                     /locus_tag="VYS74_001044"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV3789099.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            15671..15853
                     /locus_tag="VYS74_001045"
     CDS             15671..15853
                     /locus_tag="VYS74_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV3789100.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            15989..17362
                     /gene="mnmE"
                     /locus_tag="VYS74_001046"
     CDS             15989..17362
                     /gene="mnmE"
                     /locus_tag="VYS74_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV3789101.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(17409..18344)
                     /gene="dapA"
                     /locus_tag="VYS74_001047"
     CDS             complement(17409..18344)
                     /gene="dapA"
                     /locus_tag="VYS74_001047"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV3789102.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(18400..19476)
                     /locus_tag="VYS74_001048"
     CDS             complement(18400..19476)
                     /locus_tag="VYS74_001048"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV3789103.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(20062..20134)
                     /locus_tag="VYS74_001049"
     tRNA            complement(20062..20134)
                     /locus_tag="VYS74_001049"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20099..20101),aa:Thr,seq:ggt)
     gene            complement(20234..20743)
                     /locus_tag="VYS74_001050"
     CDS             complement(20234..20743)
                     /locus_tag="VYS74_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV3789104.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(20741..20837)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            20866..21297
                     /locus_tag="VYS74_001051"
     CDS             20866..21297
                     /locus_tag="VYS74_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV3789105.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            21379..21756
                     /gene="mscL"
                     /locus_tag="VYS74_001052"
     CDS             21379..21756
                     /gene="mscL"
                     /locus_tag="VYS74_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV3789106.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(21798..22892)
                     /gene="hemH"
                     /locus_tag="VYS74_001053"
     CDS             complement(21798..22892)
                     /gene="hemH"
                     /locus_tag="VYS74_001053"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV3789107.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            23047..24270
                     /gene="pepT"
                     /locus_tag="VYS74_001054"
     CDS             23047..24270
                     /gene="pepT"
                     /locus_tag="VYS74_001054"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV3789108.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(24306..24935)
                     /locus_tag="VYS74_001055"
     CDS             complement(24306..24935)
                     /locus_tag="VYS74_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789109.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            24900..25121
                     /locus_tag="VYS74_001056"
     CDS             24900..25121
                     /locus_tag="VYS74_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789110.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(25392..26403)
                     /locus_tag="VYS74_001057"
                     /pseudo
     CDS             complement(25392..26403)
                     /locus_tag="VYS74_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(26629..29748)
                     /gene="phtE"
                     /locus_tag="VYS74_001058"
     CDS             complement(26629..29748)
                     /gene="phtE"
                     /locus_tag="VYS74_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV3789111.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLVGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     SEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(29958..>30375)
                     /locus_tag="VYS74_001059"
     CDS             complement(29958..>30375)
                     /locus_tag="VYS74_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3789112.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDETEIPQVENSVINAKIAEAEALLEKVTDSSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT         8843 a         6066 c         5279 g        10187 t
ORIGIN      
        1 atgttatttc atctttagat gatttttcta cattatcatc tattctttct tctaaaattt
       61 ccattatgtt taatcttatt tcttcatcat ttaacgtttt tattaactta taaaattcat
      121 cttttgttaa atcaatactt tcttcaaaaa tactttcaaa cactgcacct ttttctataa
      181 gtcttttgtt tcttgctttt ctttcttgtt cattaagttt tttcttcaac tgtctttttt
      241 gattttgtaa ttgcttaatg ctttcttctt tcttttcaat ctgttcttca atacttaatc
      301 ttttacttgc catcatgtat cctttcttaa aataaaaaac gactagattt ttatttctaa
      361 tcgttcttat tttaaatatt ataatttgct atattgcatt ttaattttaa gtcttctata
      421 tatctttcca tgtaattcca gtctggagta cctctactgt ctatcggtaa tttaattttg
      481 atatctggca atactgtgct agatatttga tctttatatt cagtattttt taaagcttgc
      541 ctaataattg aagttaaata gaatgctact tctttattca attctttatt ttttaatttt
      601 aaacaatgaa ttttcatatc taggcttgct ttatatttat gataaaagac tgctcctaaa
      661 aatgaaacag atataaaatt ttcaaaaact ctataatttt tatcatctat atcttcgtag
      721 taacctaaga taccattgtc aatatttgta acactaattc taggagtttt tccatcgagt
      781 aaatatcttt ttggaataga tgagcctgtt ggtacttgac tcgatttccc ctttttttcg
      841 gtaataaata tttcttttta ttttgttgtc tgatattttt cctacctgtc ctttgtagga
      901 tgagtatttt ctagattttc ttgaataact ttttacttga agttttagct tttgaactag
      961 tcgttgtact ttctttttgt ttattatcag tcctgatctt tttaatattg ctgttattct
     1021 ctatatccta tttttcattc atgatattct tttactaatt ttatcttaaa ttctgtgctg
     1081 tatttgccat taaaaaactg acctccttta gttagttttt tggcctaact tttgagggtc
     1141 agttcaaaat ttgcgacttt taaatgaatt ccaatattca attattaaga gctaacatgg
     1201 tgcttgccaa taggaatcat tagaggcgaa ttggaaatag ggtcacgtat aatttttgct
     1261 tcaagattaa agatatcttt aactagttta tcatttagta tatcttcagg ctttccctct
     1321 gcaacaagtt taccttcttt aattgcaaat aggtaatcag cgtatcttgc tgttagattt
     1381 atatcgtgca aaatcatgca aatggttgtc ttatattttt ggtttagatc agtcaagagg
     1441 tctaatagtt ctatttgata tgagatatcc aagtaagtag ttggctcatc taaaagtagg
     1501 atacttgtat cttgggctag ggctagagct atccatactc tttgcctttg acccccagaa
     1561 agttcttcaa ctaggttatt tgctagatct tcaacattgg ccttaaccat tgatctgttt
     1621 attatttcaa ggtcatcttt tccaagactc ttaaaaggct ttctgtaggg gaaacgacca
     1681 cggcttacaa gatcagctac tgttattgat tcagggatta ttggagattg aggtaatata
     1741 gctatgtgtt ttgctaaatc tttttcttta taagaattaa ttgatttatt atcaagcaat
     1801 acttctccct ctaatggctt tataagtcga gacaaggttt taatgagtgt tgatttccca
     1861 caaccatttg acccaataat aactgatatt ttttcttcag gtatttttat atttatattt
     1921 tccaagatta ttttttcatc ataaccgcag gtaagattat ttgaccacag acctttcatt
     1981 atatattcct cctgttcatt tttattagta agtatattaa gtatggtgaa cctaacaagc
     2041 cagttacaac acctactgga tatctagctg gtaaaatatt ttgagagaat atgtctgata
     2101 acaaaactag taaaattcca accaatccag ctaatattgg gcttcttttc ttgccaatat
     2161 ttaaggctat gggaccagct aaaaaagata tacaagctat tggtcctgta attgaagtag
     2221 aaaaagcagt taaagataca gcgcaaaaaa ttaaaacaag ccttgaaagc tcgggatttg
     2281 ctccaagtcc gattgctatt tcttcaccaa gttcaataat ttctagtctt ttattaaaaa
     2341 ataaaactaa tatagtagca ataatactta ctattagaac aagaggtatg tcatctaact
     2401 ttgtaaaaga taaagagcca ctgagccatc tcataacttc ttgtaattca tatcttgcta
     2461 ctttcaacaa taaaaatgag gtgcctgctc ttgtgacagc ttgaaaacca atacctaata
     2521 ttatcagtct tgctgctgaa aaaccatctt ttttagctag taaaaataat attaaagatg
     2581 atgttagtcc acaagttatt gaaataattc cagtagttaa actatttgtt tttaatacca
     2641 atatgcaaaa gaccgctgca atagatgaag aacttgtgac accgattata tcaggacttg
     2701 caagaggatt tcttaacata gtttgaaaga taaatcctgc caatccaaaa gaccagccag
     2761 ctataattcc tgctaataat tttggtaatc taatttccat aatcgaaaaa ctagctccag
     2821 gaacagtttc actatttaag actttaatca aagttgaaaa agaataactt tcatctccga
     2881 taagtaaaat gaaaaatgat agactgatta ttattaataa aaatagtgag gaaaatagtg
     2941 ttattctatt ttttcttttt tgaataccta taattaaatt ttgcattagt tattaacccc
     3001 tctatttttc atagttacat aaataagtac tggacccccg attattgcag taattatccc
     3061 tacttcaatt tcacctggtt tacctaacat acggccgatt atatcacata taagcaagag
     3121 ctctgcacct ataaaagatg aagaaatggt cattgtgcgt atatctttgc ttataaataa
     3181 gccacaaaag tgaggaacta taagacctac gaagccaata ggtccaccaa ttgcagtaat
     3241 acttgaacat aaaagcacac ttgcaattat tgcaagtgat cttatcctat taacattaac
     3301 tccaagacca acagccattt catcacccat agctaaagcg tttaaatctg atgaaataaa
     3361 tatagctatc aagtgaccta aaattataaa aggtagtagt gtagatatag aagataatgt
     3421 agctgctcca aggctaccta tttgccaaaa tctaaatttg tctaagacgt tattattcgg
     3481 taaaattaaa aaacttacaa aactgcttaa agccatacta acacaagttc ctgataaggc
     3541 aagttttata ggggtaaggc ctgcttttcc gcttacagca atcgcgtata caaaaattgc
     3601 acttactaag ccaccaatga ttgcaaaact tatatggctt atgcttgatg aaattcctaa
     3661 aaaagaaaga ccaattacta cactaagact tgctcctgtg tttataccga gtatacctgg
     3721 atcagctatt gggtttctag taactgattg catcaataca ccgcttatgg caagactaga
     3781 acctgctaaa attgcaaaaa tagttctagg tattctttta tatataattg atttaataaa
     3841 atcatcattt gtattaccca ttccaaaagc tgctaaaaaa tctctgatat taatttcttt
     3901 tgttccaagc tttaatgaca aaaatattgt aataagaaga cctaggcaga ttacaaaaat
     3961 tatgcctagg tttatttgtt ttttatttcg catttttgca tgcatttcct aaaagattta
     4021 ggtattcttc aatagtatag tttattgaaa gtggtgttgg agtgcatgag gctgctaacg
     4081 gtgtattatc tggaattaca gcaacggcac catttttaat tgcatttatt ttacctaaaa
     4141 gaggatcttt ttgtaaagct tcaagagttt tatcatcacc ataagttatg attacatcag
     4201 catcatttat cttatttgct tcttctgcag aaatttcctt tgcaaaacta tcttcactct
     4261 caaattcttt taatgattca gggaaaacta gacctaaatc tgttaaataa tttgctcttg
     4321 gatctttgct agtataaatc cagaattttg atgtatctgc agcattaatc atagtaaata
     4381 atactttttt tcctttgatt tttcctttga tttctggatg tttttctaat tctttggata
     4441 tacgagcttc agtatttttg attaactcat caccttcttt ttccatacct aaggcttttg
     4501 aatcaatttt aatcatatct ctccataaag tttgccaagg tttagatttg tatgctgcta
     4561 caggagcaat ttttgataga gtgtcataat cttctttagt tataccagaa taacctgcta
     4621 agataacatc tggtttagaa tttgatattg cttcaaagtt aagtccatcc aaatcgtcaa
     4681 ataggttagc tttaccattt agttctttga ttttttcttc tgtccatggt aaaactcctt
     4741 tatcagcact tacaccgtaa tttgcttttg aaaatccaac aggaactatt cctaaagcta
     4801 atgctacatc atgatttccc caagcaatag ttgcaactct ttcaggtttt ttatctaata
     4861 tagtttgacc gaaagcatga tctaaaacta ttttatcagg cgcatgctct ttagaagtat
     4921 tttcttcatt tttaacagaa ttagaagaac aagcgctaaa aactatacac atagctaaaa
     4981 tagctattag aaaaaatttg tttttcataa aaactcctta aacatatttc aagtctattg
     5041 tatcgataat aattatcatt gtcaatagat acagttacaa atttaataat ataatttttt
     5101 caaaaaagga tctagttttt atttttagtt aaattatgtc tatatttttc attaactcat
     5161 caataaaaat ttgtttgcta atatctcttt cttctgattt tgtttcaagg attattgcta
     5221 catcattcat atttttaggt aaataccaac catctggctt atctgaatat cccttgaagc
     5281 ctaattgatt aaaagtagtt atttgaccag taccttgatt gatgttttct tcaacttcat
     5341 taaagcctat acttctcttg cataatccct aacttgatct tctgtcctta aagtcatttt
     5401 ctattcctcg tcactaaaat tatcttcaaa actaataata tcattaggag tgcaagataa
     5461 agccttacat attctttcta aattcttaaa tgtaattggt ttatcctttc ccatttgtgc
     5521 cataacattt gttgtaagtc ctgccatagc tattacatct gttttcttta gtcctttttt
     5581 tgctaactgt atccataatg gtttatagtt aagtgccata atatccctct ttttttcttc
     5641 attagattta tttaattata gcataaatat tgatttcatt caatcttaca ttgatttagt
     5701 gatttgtttt agagaatttg ttgtgttatt ccgtccttat tttgccataa attgttttaa
     5761 gtgggacctc ttatccttac catttttacc tcctaatttt gagtataaaa aagacgatag
     5821 agtttttaat ttctatcgtc tgagatttac atattaaatt ttcatcacaa aataagactc
     5881 tttaaagaat ctttaaattc attggtatgc taattacaat atccattatt aaaatggata
     5941 ctataagcag cacaccaact atttattttt tgagttttaa actttggtag aagtcttgaa
     6001 ctccttatat ttacataact atttgcctct ataactgtta tgacacgaaa ccaacaagca
     6061 agttcaagca cctccatatc tttattcaaa tgaggactaa gatattcaca aactttatca
     6121 taaacctctt tatctttttt cataaaaggg gcatacaact tagcaaattt atcctaaaat
     6181 tttttgttgc ctttatcaat catttcaacg ctcctttaaa tataaaaatg atcctatcaa
     6241 tctttatatt gagcctattt tctaattgat accttgcaag aaaggaataa tagctgttcc
     6301 tataccaagt aggaatgtaa taaatgcaga aagtcatata ctgtcattct tttctattaa
     6361 atcattttga agtgtctcaa agcatccatg atggcttctt ctttttcagg tgtacactgt
     6421 ggaataattt gtttatcctt tttagacttg ttgtaatgtt ctcgtaattc tattccacat
     6481 ttctttttta tctgtgcaat atataatgtc gaaactttta attcaaattt attccaaaca
     6541 tattctttga tttgagcata tgttgctttg ctctccgcac ttgtcaaatc catctcatcc
     6601 agctcaattt caacacttat gtgcttatcg acatcgagtt tggacaaaag tgctaccgtc
     6661 tcgacgtgat gcgtttgagg aaatagatcc accggctgga ctttcttcaa ttcatatccc
     6721 aactcttggt atagtttaat atcacgcgcc atggttgcga cattgcagga gatataggcg
     6781 atgcgatcgg ctcctgtttg ggcgcttgct ttgataaagc tttctgtcaa gcccttgcgt
     6841 ggaggatcaa ccaagataac ggttggttga atcccatctt taagccaatt cttcatagca
     6901 ttttcagctg tgtcacagac atagtgggcg tttgaaatat tgttcagctg agcatttttt
     6961 ttactattct caaccgcttc tggaatcagt tcaacaccgt agacttcttt gacatgcttg
     7021 gcgactgata aaccaatggt tccaatacca gaataagcat caataaccac gtcatctttt
     7081 tttaactctg caaagtcaat ggctgtttga tagagtttct ccgccatttc agtattgact
     7141 tggtaaaagg ctgggccagc gatttggaag tcatttccca acatctggtc cgtaatatag
     7201 tcttgaccat aaagagtgcg ccactcctta ccaaaaatcg cattggtatt ctggtcgttg
     7261 atattttgca tgacagacac aatctctggg aactgcttga taacttgttc aatcaattgg
     7321 tcaacacgaa aaacttttgg acgagttgtc accaaaacga ccatgatttg ccctgaatag
     7381 tgaccacgac gcaccacaag attccgaatc aatccagaat gttccttttc gtcataaggt
     7441 tttaaatcaa aacgacggaa caggtctcga agaactacta cgacttggtc aatgacagga
     7501 tcctggataa agaaatcttc aaggggcatg aggttgtgcg aattattacg gaaaaatcct
     7561 gtttccaaga caccattcac tcgacgaacg ggcacctgcg ccttattgcg atacttgact
     7621 ggatgttcca taccaagcgt ttcagcaact tctacatctg caattccagc aatcttgtag
     7681 agactgtcct tgacttgctt ggttttaaac ttgagctgtt ctggataaga aaggtgtcct
     7741 aaatccgcga ttcctgaacg caggtaagcc aaatctagat cttgattacg gtgtggtgac
     7801 tggacaaggt atttttcaac ttttccaaag ccaatctttt tattgacctt gaggacacgc
     7861 atgagaattt tttcactcgg taaagcattc tctacaaaaa agaccaaacc atctaccttg
     7921 gcaactcctg ccccttcatg ggtcaaatca acaatttcaa cttctacaat atcatttttc
     7981 tttaacattc ttttctcttt ctattggccg acaaaaaaga cggcaacgtt acggttacca
     8041 tccattatac catgaaattt cttaagaacc aaaactcttg aagaagttga caagggcttg
     8101 ccagagggag cttaagaagt taccaccgtc ctctagcgcc ttatccgcat caaagttaag
     8161 gttgatattt ttaaaactgt cgccagcttg tgatacgatg ctttgtttaa ggtcatttag
     8221 ggttttagtg aaatctgcat tgctgaggat atcactcttt gagagattca aggcaaaatt
     8281 gatgatgata ttgatctggt ttcctgttat gacctgatca agtttgtaat tttttaaggt
     8341 atcttcaaca atcttgcgta catcttcttc tgtcagattt cccttgcttt ctttagcttt
     8401 ggcgagtcct gacttgatat cagctagggc aacgtttaat ttattagcat catagcctga
     8461 tttgtccttg ttttcagcat tgatatctga caaagccttt agctcttctt gagccaaatc
     8521 tttattggct tgcggcacct tggctccatt agcttctagc gaatagtaga tcccagccaa
     8581 agcactctca cctgtaactg gaataggggc tgctacagtg attttggcat gttccatacc
     8641 cagcgttact gctgcgtttc ggtacatatc ctgagtcacc ttagtgatat tttctggtgt
     8701 ttcaatcttg acctcaagtg gcgatttgtc acctagcttt tgaatcttgg ctgatgaata
     8761 caactgtaag ctagagtcat tggccacatt catgatttta gaataaacat caggtgtcat
     8821 ggtcttgagt tctttggtat ctgttgaggc attgtagccc agttttttaa gagtttgatt
     8881 tttttggtct tcagataggg aggaacctag gacatattca ggttggacat aggtttcatc
     8941 gataactttt tgaacatctg ttgctgcatg ggcgctattc atagctgtta ctgcccacaa
     9001 gaccgcagca ccggtcagaa agagtttctt tctcataggg aatttcctcc tttacttttc
     9061 tagagtaata tatctatctt aaagaaaact tataacaaaa acacctggtc tagccagatg
     9121 ttgaaaagag agtgaaacat ttgatgatgt aaaggttaag tcgtacctgt ctagaataat
     9181 aatagtttcc tccatttaca tagagttcag caccgtgaaa aatggaaatg gggtgaatat
     9241 aactataagt ctttccagtc ctattaccaa gcaagggggc aacagtctca cgagagtact
     9301 gtttggctag agccaaggta ttttccttgc cattttgggc gataaaatcg atataggcag
     9361 gtccaaaatt ataggcttga acagctgtcc agatatctac acccttcttc tgcgccagat
     9421 agagattgcc tgtcagagtt tgaatgcctt gccgaatgct agaggcatta tcattgatgg
     9481 tgttggtgga accacttgca gactcactag actgcataac atcgccttct tttccttttg
     9541 tttcagtata aatcatagca agcacaagct cttcgtttgc tggggtgtct ttttcactca
     9601 atatttctcg caccatgggt tgataggtca tgacttgttt gacatcttga tgaacgcggt
     9661 aagctttata gccagcaaaa aggaagactg ctagtacaag cactcttcga attcgtttaa
     9721 acattattta ctttggatat cctcgatatt tttgattaag atagagtagg ttccattttc
     9781 gttttggata aactcaacag actcggcgtc ttgatagacg ttattgggaa cgatgagctc
     9841 aattccattt gataaggaga gtttttggtt ttcaaatttc tttaattggc gactggcatc
     9901 aatttcatca aattgaacag gttctggtac ggcttctttg acttggtcaa taaagctcaa
     9961 acgagccgtc agattgttgt caaaaaggtc attagccaat ttctcaggtg acaattcatt
    10021 gctttcttct aggttgttga aaatagctga tttgaccttg gattgaaatt gaaaatcatc
    10081 tgtgttaaaa gattcagcaa ttctctgggc tgttttttcc agttccttga tagatttttt
    10141 aggagaaatc ttaggagcga cagcaagaag attatctgaa aaatagttca aaaaagtccc
    10201 gttgtacttg attcgttttt caatcaggtg atacttgcga ctctgaagat tgaccaccaa
    10261 ggcctcgtca gcacccgttc caaatccagg caggttattc tgagtcagct tgattggatt
    10321 atcaacttct cctccgaggt gggtcaaggt ctcccgcagg gcaattcgca agaaagcgaa
    10381 atgttctaca ccttctttag aaaattgaac aaaaatcaag tcattggtct tgagattttc
    10441 agaaatgcta aactcctctt tccagagatt agccagcgtt actgatgtct ccaacaaatc
    10501 gtctgtaata tgattgaaga agggattttc ttcttcgaaa atcccagtct tggcttcatc
    10561 tgaatacaca tgttcaattt ttttacgtag gtattcttcg atttttggag taatattgag
    10621 aaacttatct gctaagaaca gctcggtatc atccggactg aactggtgaa taatggcttt
    10681 cttaatataa atatccataa aagttttagt cctcgtataa tgggaaggca tctgtcaatt
    10741 ctttgactgc acttctcact tcttctaata cagcctcatt ttctgaattc ttaagggttt
    10801 taatgatgag ttcagccact ttgcgacttt cttcttcacc aaatccacgt gcagtaatag
    10861 ctgctgctcc gatacgaatc ccacttgtct tgaatggcga caaggtttcg taagggattg
    10921 aatttttatt tagggtaata ttgacttcat ccagcaagtt ttgagcaact tttccgtttt
    10981 ctacaacctt agtcacatca acaaggaaga gatggttttc agttccacct gaaataatac
    11041 ggaaatcagg gtcttgcaag aagacatctg ccatagcctt gctgttctta attacattgg
    11101 cagcatattc cttgaaggct ggatccaaaa cttctttgaa ggaaactgcc ttagccgcca
    11161 caacatgctc taaaggaccg ccctgaatac ctgggaaaat agctgaattg atttttttag
    11221 caagttcttc gtcattggtc aaaatcaaac caccacgagg tccacgaagg gttttgtggg
    11281 tcgttgttgt tgtgatatga gcgtatggaa ctgggcttgg atgaaggcca gccgcaacca
    11341 aaccagcgat atgggccatg tccaccatga gcttcgcacc gacagcatct gcgatttcac
    11401 ggaattttga aaaatcgata atttgagaat atgctgaagc accagctaca atcagttttg
    11461 gttttacttc ttgggcttgt ttcaagatag catcaaagtc taagagttcc gtttcaggat
    11521 caacactata agaaacaaag ttgtaggttt gaccagagaa gctaacagga gccccatgag
    11581 tcaaatgacc acctgatgcc aaatccattc ccataaccgt atcacctggc tcaatcaagg
    11641 acatgtaagc cgcacagtta gcttggcttc ctgaatgtgg ttggacattg gcaaatttag
    11701 caccgaaaat ttcttttgcg cgttcaatag caagagtctc tacaacgtct actacatcag
    11761 ttccaccata ataacggcgt cctgggtaac cctcggcata tttatttgtc aagatagacc
    11821 cttgagctgc cataacagcc ttggaaacta cgttttccga agcaattaac tcgatattat
    11881 tttgttggcg ttcttcttct ttggcaatag cattccagag atcagcatca tatgctttaa
    11941 aatcatcttt gtcaaaaatc ataggtcttc tcctttattg tgtgactagt ccattagttt
    12001 gattttacaa taagaaaatc aaactaaaag atgcgaataa accgtttctg cattttatca
    12061 caagtatagc caactttttc ataaaatgca tgagcaccca gacgatgatt ggcagaattt
    12121 aagcggataa acccataacc acatcttttt gcttcttctt ccaacccttg tagtaaactt
    12181 ttaccaatac cttgaccttg cgcttgaggt gaaactgcta aagctaagat attaaatcct
    12241 gctttggaat agagtgattc gtaaacttca gcgtggacat atccaagtaa gacatgatta
    12301 gctgcatcct catagccaag taggaaatga tgggaatcct gagacagtct agctagttgg
    12361 ctagccgttt cctctggact aaaagtataa cccaaagcct cttggttgat gtcacatatc
    12421 gctttcacat ctgtttcttg caaatctctt agcatttcaa gcctcctcaa aagaaatctc
    12481 tggcaaccga gcaagaatat cttctcgttt aattgcgcct tggcgtaaga ttttcacctt
    12541 gtctccagac aaatccacaa tagttgaatc ctgtccagtt agaaaagcat cgtcttccag
    12601 acccagaacc tcttggtcaa aatccttcag aatttgttca aaggttacac cacttgcctg
    12661 acctgagata ttggcagacg gcccaatcaa gggacctgtc tctcgaatta aatccagtgt
    12721 gatagggtga ctgggcatcc gaaatccaat agttgcaagg tcagaattta cccaataggg
    12781 aactcggtca ttggcttcga gaataatggt caagggacct ggcaaaaagg tctctacaag
    12841 tttttgtaga taagttggct gattcttaga aaagtgcaag atatcctcga aagaggcgat
    12901 attgagattg agtgccttat ctctaggacg acgtttgagt tggtaaacat ggtcaactgc
    12961 tttttcatct aaggccttgg caaaaagacc ataaacagtc tctgtaggta gaacgacagc
    13021 tccaccattt tccaactctt gtctaatcct gtccatcatc aaccacaacc atcctatttt
    13081 gaccaaattg gtctttgagt gttcgaactt ttttttcagg aagatgtttt ctaaaaagtt
    13141 cgggaacact ttgaccttgc ttgtatccaa tttcaaggta aatcttacca ccatccttga
    13201 gatagtcttt tgcatcttcc gcaattctac agtaaatagc taggccatcc tcgtctgcaa
    13261 agagagctag atgaggctcc gaatgcaaaa cattcaaacc tacctctgac tcatcttcac
    13321 gagagatata gggtggattg gatacaatta tatcatattt ttcagaaatt tctgcaaaac
    13381 aatcagattt tttaaaaaat atattaagat tttgatcgct agcattctct gatgcaagct
    13441 ctaaggcctc ttgagaaaca tctgctgctg tcactgacca atctggtcta ttttttgcta
    13501 gagcgagagc aatagctcca ctacctgttc caatatccag aactgagaga ttcgtctcaa
    13561 gattttcagt tagaataagc tccactaact cctctgtttc tggacgagga atcaaaaccc
    13621 gttcatccac ctttaaatgc attccataaa aatctgcctg tccaatgatg tactgagctg
    13681 gcttgtgagc tgctagttgc tggtaaatat cttctacaaa ttgtttttct tcctctgttg
    13741 tcacctcctg ctggagggca aaaataaagt ctgtaaaaga tagatttttc agactacgat
    13801 agacaaaaga gaggctttcc gcttcctctc cttgtcttat caactcttct tcaaaatttg
    13861 aaaataattg agctaatttc attatttgtt taattcttct agtttttgtg tttggtcata
    13921 aagcaccaag gcatccacaa cttcgtccaa tttaccagac aaaatcgtat ctagtttttg
    13981 gagggtcaag ccgatacggt ggtctgtgac acggttttgt gggaagttat aagttcggat
    14041 ccgttctgaa cggtcaccag taccgattgt cgacttacgc tcagcgtctt gttcatcctg
    14101 agcaatctga gcaaagtggt cagcgacgcg tgcacggata atcttcatag ccttctcgcg
    14161 gttcttctgc tgggtacgtt cttcctgcat ctcaaccttg atattggttg gcaagtgaac
    14221 gatacgaacg gcagtcgcaa ccttattgac gttctgtcca ccagcaccag aggcgtgata
    14281 gatgtcgaca cgaaggtctt ttggatcaat gtcgtattca acctcttcaa cttctggcat
    14341 aacaagaact gtcgctgtcg aagtatgaac acggccttgg ctttctgtca caggaacacg
    14401 ttgcacacgg tgggcacctg attcatactt aagcttagag tatacagact gacctgaaac
    14461 catagcaacc acttctttaa aaccaccgac accattcata gaggcttcca tgacttcaaa
    14521 gcgccaacct tgggcttccg catacttttg gtacatagtt agcaaatctc cagcgaaaag
    14581 tgccgcttcg tctccaccag ctgctccacg gatttcaagg atgatattct tgtcatcgtt
    14641 tggatccttt ggaaggagca aaattttcag tttttcttca tattcttctt tttcagcctt
    14701 ggcatctttg agttcttgct tggccatttc ttccaagtcc gcatctccgc ctgattcctt
    14761 aatcatctct tcggcatcga cgatattttg aaggacttgt ttatactcac ggtaggctat
    14821 tacggtgtca cgattggaag cttcttcttt tgaaagctcc ataaaacgct tggtgtctga
    14881 aacgacatca gggtcactca gcaattctcc tagttcttca taacggtctt ctacagcttg
    14941 tagttgatca tagatgttca tttttcttca ttctccttat tgatttcagg ggcaaaataa
    15001 tgtttacggc aaaccgagat ataggtttca ttaccaccaa tctggatctg ttctccatcg
    15061 taaacgggca gtccatcctg tgttcgcaac accatggtcg cctttttctt gcaatactga
    15121 cagatggtct tgatttcgtc aatcttgtct gctaaaagca agagatattt ggaaccttcg
    15181 aacaattcat tgcgaaagtc atttttcaag ccaaaagcca tgacgggtat gtctaactcg
    15241 tccacaacac gagctaggtc ataaacatgg tggcgtttga gaaactgggc ttcatcgacc
    15301 aaaacacagt aaggtttttc tggtaggtct cggatatagc caaagatatc cgttgtttcc
    15361 tcaatcgcaa gggcagggcg tttcatgcca attcgactcg acacatagcc aacaccgtca
    15421 cgcgtatcca gagccgaggt cataatcaca acaccttttc cttgctcctc gtagttatag
    15481 gccactttga gaatctcaat cgttttacca gagttcatgg tcccataacg atagtacaac
    15541 tgtgccatgt ttcttgcttc acgtccattt ctaaattttt gctacattct agtatatcat
    15601 aattttctta agctttaaac ggcaaaatgt ggtaaaatgg aagaaatcaa aactagtgga
    15661 ggaagctatt atgccatttg tacgcatcga tttatttgaa ggacgcacgc tcgagcaaaa
    15721 gaaagctctt gctaaggaag taacggaagc ggttgttcgc aacactggag cacctcaatc
    15781 tgctgttcat gtcatcatca acgacatgcc agaaggaact tatttcccac aaggggaaat
    15841 gcgcaccaaa taagctagct taagcagaat tgcttaggct ttttcaatct ccaagtagca
    15901 cccattgaag aaatagtcta aatttgttac aatttgaaaa gagacttgga aaattccaag
    15961 aaaagagcaa ttaattaaag gaaacattat gattacacgt gaatttgata ccatcgctgc
    16021 tatctctact ccactaggtg aaggggctat tggtattgtc cgcctgagcg gaacagacag
    16081 ttttgctatt gtgcaaaaga tttttaaagg aaaagacttg aacaaggttg ccagccacac
    16141 tctcaactac ggtcacatta ttgatcctct gactggtaaa gtcatggacg aggttatggt
    16201 tggggctatg aagtctccaa agaccttcac tcgtgaggat attatcgaga ttaacaccca
    16261 cggtgggatt gcggtgacca atgaaattct ccagctagct attcgtgaag gggctcggtt
    16321 ggcagaacct ggtgaattta ccaaacgtgc ttttttaaac ggtcgcgtag acttgacaca
    16381 ggcagaggct gtgatggata tcatccgtgc caagactgac aaggccatga acattgcggt
    16441 caaacaatta gacggctccc tttctgacct cattaacaat acccgtcaag aaatcctcaa
    16501 tacacttgcc caagttgagg tcaatatcga ctatcctgaa tatgatgatg ttgaggaagc
    16561 tactactgcc gttgtccgtg agaagactat ggagtttgag caattgctaa ccaatcttct
    16621 tagaacagcc cgtcgcggca aaatccttcg tgaaggaatt tcaacagcta tcatcggacg
    16681 tcccaacgtt gggaaatcca gccttctcaa caacctcttg cgtgaggaca aggctatcgt
    16741 aacagatatc gctgggacaa cacgagatgt catcgaagag tacgtcaaca tcaatggtgt
    16801 acctctcaaa ttgattgata cagccggtat tcgtgaaaca gatgacatag tggaacaaat
    16861 cggtgttgag cgttcgaaaa aagccctcaa ggaagccgac ttggttctac tagtgctaaa
    16921 tgccagtgaa caactgactg cgcaagacag acaacttctt gaaattagcc aagataccaa
    16981 tcgcattatt ctacttaata aaaccgacct gccagaaacg attgaaactt cgaaactacc
    17041 tgaagacgtt atccgtattt cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg
    17101 aatcaacaac ctcttctttg aaaatgctgg cttggtcgag caagatgcta cttacttgtc
    17161 aaacgcccgt cacatttccc tgattgaaaa agcagttgaa agcctacaag ccgttaatca
    17221 aggtcttgag ctggggatgc cagttgattt gcttcaagtt gacttgactc gtacttggga
    17281 aatcctcgga gaaatcactg gggatgctgc tccagatgaa ctcatcaccc aactctttag
    17341 ccaattctgt ttaggaaaat aagaaaaatc catgatcctt cattcggtca tggattttat
    17401 tgtctttatt agtaatctgg tcttaagacc cctgttacag ttgccttagt tgcttcgtag
    17461 tcgccatcta cgacaacctt gataatgcgt ttggcatctt cttctggtgc tggaacaaga
    17521 ggtagacgag tgggtccagc ttcaaatccc atatagttaa gaattgcctt aactggagca
    17581 ggacttggat aagagaagag agcattaacc ttaggaatga atttacgctg aattgctgcg
    17641 gctttcttca tatcgctttc tgcaatggca gtaaacatct cgtgcatttc atccccattt
    17701 gtatgagagg caacagaaat aaccccatcc gccccaaggt tcatggcatg gaaagcatct
    17761 ccatcctcac ctgtataaat caagaactct tcaggcttgt gctcaatcaa gtaagccata
    17821 ttagccaagc tagtacattc tttgacaccg ataatatttg gatggtcagc caagcgaagc
    17881 atggtttctg gagtcaattc gacaactaca cgccctggaa tgttatagat aataattggt
    17941 aggtcagaag catctgcaat agccttaaag tgctgataca tcccttcttg agaaggtttg
    18001 ttgtagtaag gaacaatagc aagcccagct gcgaaaccac caaattccgc tacttctttg
    18061 acaaactcaa tagagtcacg cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt
    18121 ccattgacaa ccttttgtac agccgcaaac aactccaact cctcatcgtg ggtcaaagtt
    18181 ggactctcag cagtcgttcc tgcgagaaga attccatccg tatgatgggc caataaatgc
    18241 tcaatcaagg ctggaatagc atcaaagtta atggaaccat cctcatggaa gggggtaata
    18301 aaggctgtaa tgattttaca ttcttttaaa tcttgataag acacgaaaac acctctctat
    18361 ttcaaagaag gagttcatct gaactcctaa acgatatgac tattttaatt caaatttcaa
    18421 ttcagctgtt ggacgaacca atccacgttc atgaagagtt tcagcaatct gaactgagtt
    18481 ccaagcagca cctttgagaa ggttatctga aacaacccac atgtgaattc ctttttctgc
    18541 atccaagtct ttacggatac gaccaacaaa ggtatcacgt gaaccaactg cattgatggc
    18601 ttgaggatag atttgatgaa ctacatcatc ttcaagaaca gcacctggga aggatgcgat
    18661 agctgctttg acttcttcaa ttggagccac ttcctttgtt tcaatataaa ctgactcaga
    18721 gtgagctgac aagactggaa tacgcacaca tgtcgcagat actgcaatgc tatcatcttc
    18781 cataattttc ttagtttcct tggtcatctt catctcttcg tacgtgtaat cattatcagt
    18841 gaagacatca atttgtggaa gagcgttaaa ggcgatagga taatgtttct tgtcaccacc
    18901 tgaaggcaag atttccgcat gcaaatcacg tggtttcact ccatcattca agacctcacg
    18961 aagttcacgt tgtgtctcaa gaattgctcc cataccagca cctgaaacgg cttgataagt
    19021 tgaaacaatg atacggtcca agccccattt ttggcgaacc ggctcaagag ccaccatcat
    19081 ttggattgtt gaacagttag ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc
    19141 attgacctct ggaacaacca aaggaacatc tggattttga cggaaataag atgtattatc
    19201 cactactacc acgccagctt ttactgcgta tggtgcatac ttagctgatg tcgaaccacc
    19261 tgctgaaaag agagcaatat caactccttc aaaagctgtt tcagtcgttt cttcaatcgt
    19321 aatatcttga tctttaaatt tcaatgactt acctgctgaa cgtgcagaag caaggtaacg
    19381 gattttatcg attggaagtg ttgattcttc caacattttt atcatctgag caccgacagc
    19441 acctgtcgcg ccgactacag caactgtata tcccataaat aacctctttc ggaattttct
    19501 aaaaatttct ataatagaag tattatacta ctttttccat gaattgcaaa gctttttcat
    19561 cattttttgg aaaataaaaa tccccaatcg ctagactagg gattaagagg catcttcatg
    19621 tgatgagcag gttcacacaa ctcatcaagg tccgctcctg cgttatgacc tccttatgct
    19681 caatagtagc ccgaagacta cctatcgact catcgcatct actattctac taaagagaat
    19741 gacttttgtc aacagacgaa actctttata ctcttcgaaa atctcttcaa acttcgtcag
    19801 cgtcgccttg ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaaac
    19861 atgttttaag ctgacttcgt cagttctatc tacaacctca aagcagtgct ttgagcaacc
    19921 tgcggctagt ttcctagttt actctttgat tttcattgag tattagtttt atttacacag
    19981 gtgcataaga aatatattgg aaaaattggg ggcagacata acaaaaaccc ttgtaaatca
    20041 agggcttttc tgtctattta atgctaattg ccgggattga accggcgacc tcatccttac
    20101 catggatgcg ctctgccaac tgagctaaat cagcttactt aagaagtata ctatattcag
    20161 aaaaacttgt caagtttcct tagaaatttt ccataggaaa aagccaggta agaactacct
    20221 agcttatctt cttctattta cttaaatcaa ttcgacgacc aattttacca ataataatcg
    20281 ctccaataat caatgaagca aattcaccga ttcctgttgt aaaccaagta aggaagaatg
    20341 gtaattctgc tacaatattt aactctgcag cgatagtaaa catggaaatt gaaaagagga
    20401 ttgaaaagaa gaaatgatct tttcgaatca atccgttaaa gaggtaatcc ttgctatatt
    20461 ttgcaaaaag ccaaacacct aaactgagga atactaaggt tgaaccacca ccaacaaaga
    20521 catctagcag tccgaagcta aagaaattag caatcataca accgatggta actccgatga
    20581 tgtacttagg attgtaaaaa gccataaagt tcatcatttc tgaaatacga aactgataag
    20641 caccatagct gatggcattt aggggcggtg tgacagtcaa aacgacgtag atagcagcga
    20701 cgattgcaat atctgcaaca tcacgaatag ttaatttttt catcttttct cctttggcgg
    20761 ttatccgcgt gtaatatgct tggtgaaaga agctaagcac caagggttga ttcattcaac
    20821 cttactagta tagcacaata gcctgcttta tgctatactt aaagcatgaa aaaacctatt
    20881 caaaaatttt ttaacaatga aatcttagcc tatctctttt ttggccttgc gacaactcta
    20941 gtttcgattc tctcacgact agtcatttat caactaagtc atcaggaact ccttgctacg
    21001 gccttggcaa atattatcgg tatcttattt gcctttatca caaatgatac gattgtattt
    21061 aagcaaaaaa agaaaaatag acttatccgt ttagtgaaat tttctcttgc tcgcctttct
    21121 acttttctgt tagacttgtt tttaactttt ctctttgtaa cccaatttcc tcacatcata
    21181 gggcaattcg taactgaaaa cattgataag ataaatgcta ttgaaacaat aattgcacaa
    21241 atattcatta ttatcatcaa ttatatcttg agcaaggtct atatttttag aaaataatgc
    21301 tccatttatg agccttttaa ttgccttcta taatgattta ggatacaatg aaacacgaaa
    21361 cattgaaagg aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt
    21421 attgaccttg ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt tacttcactt
    21481 gtaaacgaca ttatcactcc tcttatttta aatcccgctt tgaaagctgc taaagtcgaa
    21541 cgtatcgctc aactttcttg gcatggagtc ggctatggta actttttaag tgctattatc
    21601 aattttatct ttgtgggtac cgccctcttc tttattatca agggcattga aaaagcacag
    21661 aaactgactg gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga attggaagtc
    21721 cttcaagaaa taaaagctct ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa
    21781 gctaaatcct tttttcttta ctctttgggt aacttgcctg ctttttcaag catttttttg
    21841 ataagataag gcatcttaac attctcccgc ccttttttct caatcagttt tttcacaaat
    21901 tctggcattt gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc tgaaggaact
    21961 aactcatcaa aggtctcttc ttctgggaga aattccttaa actcttgatt ttcattgact
    22021 ctaactgtat tgaccaaagc atcaatcaaa cgagaatctg tatttggcat tggtggacga
    22081 tggtagttta ccccaaattc ctggcataag tcataacatt ctacatcatt gtcaaacaag
    22141 acctcaatat gctcactgat gaaactaata ggtacaaaaa tataatggtc tggatgttct
    22201 gtctgttctc tgagatactc caaaacatct ggtttaatcc atggaatacc aatatcactc
    22261 tcactctgcc aagtgttggt atattgttct gaactcaagc ccagtttttc agcgactagc
    22321 ttgctatttt cgaaaatttg gtctatataa ggatcaccaa agtccaaggc aaaaatgggc
    22381 acactatggg ctgaaaagat gactttaaag ctgtcttgct ttacttcttc ttttaaaatc
    22441 ttagcaattt catctgccca atagtttaag agcgcttctt cctgatacca gtccttaatg
    22501 accaaaaatt gaatttgttt gctttctaaa aatttttcat agcccatgac agagtagaaa
    22561 gaataatggg gctccaaaat caagcaaata cactgttcaa ttccgtctgc ttccatttga
    22621 ccgatcacat ctggaataaa gggactggaa aacttattag caaagtagac actgtattca
    22681 ttccccagcc tagcttctac caaggcaacc tcttgacggg taattttttg cagaggcgtg
    22741 cctccaattc gtacataatt atcatagaga gtctgaatct cgtggtcttg gggtctcacc
    22801 ccacgacgaa tatttgtgaa aaaatcagct acaccttcaa aggtaatctc ttctggtgaa
    22861 ccgaaggtca tcattaaaat tgcttttttc atgactgctt ccttgtgatg tttttatcaa
    22921 gtttatttta tcattaatta atcgataagt aaacgctaat atagggacat tctcaatctt
    22981 ctgtcttttg tttttctctt ctttctatga tacaatggaa aaaataaatt caaaaggagt
    23041 ttttttatga cttatccaaa tctcttggac cgcttcttaa cctacgttaa ggtcaacacg
    23101 cgctctgatg aacactctac tactactcca agtacacaga gtcaggttga cttcgcaaca
    23161 aatgtcctaa ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta tctaccgaat
    23221 ggttttgcta ttggaacctt gccagccaac gatccgtctt taacacgtaa gattggtttt
    23281 atatcgcaca tggatactgc tgattttaat gctgaaggag tcaatccaca ggtaattgaa
    23341 aactacgatg gtggtgtgat tgaactaggg aattctggtt tcaaactcga tccagctgac
    23401 ttcaagagtc ttgaaaaata tccaggacaa acgctcatca caacagatgg aacaagcttg
    23461 ctaggtgctg atgacaagtc aggaattgct gaaattatga cagccattga atatctaact
    23521 gctcatcctg aaattaagca ctgtgagatt cgtgttggtt ttggtccaga tgaagaaatc
    23581 ggtgttggtg ccaataaatt tgatgcagaa gattttgatg tggattttgc ctacactgtt
    23641 gatggtggtc cactaggtga acttcagtac gagactttct cagccgctgg tgctgaattg
    23701 catttccaag gccgtaatgt ccaccctggt actgccaaag agcagatggt caatgccctt
    23761 cagctagcaa ttgattttca taatcaactt ccagaaaatg accgacctga gttaactgaa
    23821 ggttaccaag gtttttacca tctaatggat gtgacaggta gtgttgagga ggtgcgtgca
    23881 agctacatca ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc atccatgcaa
    23941 tctatcgctg ataagatgaa tgcagaactt gggagctacc gtgtcactct caacttgaca
    24001 gaccagtact acaatatgaa agaagtcatt gaaaaagata tgactccaat taccattgct
    24061 aaagccgtta tggaagatct aggtatcacg cctattatcg aaccaatccg gggtggaaca
    24121 gacggctcta agatttcctt tatgggaatc ccaactccga atatctttgc aggtggcgaa
    24181 aatatgcacg gacgttttga atacgttagc cttcagacta tggaacgtgc agttgatacc
    24241 atcattggca tcgtagctta taaaggctaa aaagacgagg tagctcagct acttcgcctt
    24301 tctttttatt ctactggttt ttcttgattt ccagtagttg tagaagattc tgttgtttca
    24361 ttttctgaag ttgattcagc aggtttagaa tctcttgtat tgcttggttt gttttcgtcg
    24421 ctagcaattt caatgttaga ttctgcagtt gcgtttggtt ggttctcagc actggtgtta
    24481 tcactatttg cttcagcatt tcttgctgga cttgtttctt cacttgcgct agcttttgac
    24541 tggatttgat gattcaaaac tagaatagct tttgtcgatt caagtaaagc tgttttgtct
    24601 ttactcttag cagaaagttg atctaataat gcatccacct tatcaaagtc cgcatcagat
    24661 ccattattac tttctaaata agagtgaagc gacatgagaa tatcgtagag tttttgatag
    24721 agtacaagtg tctgagaatc ttgctcagca ttttcctttt cttgttgaag ggcgctagcg
    24781 atacgagtca agacatcttt tacctgactg tttacttcat ccaagtctgc atcagccttg
    24841 tttgtggcag cttttagatt ttctacttct tctgccaagg attgtctgat tccttcttca
    24901 tggatttgtt ccaagagttg atttaccttg ctcaaaagac tttctacttc ttccttgcta
    24961 tctgtcgcag attattggtt gccatctacc atgtactcct aaaacaggag agttataatc
    25021 caagattaca aggccttaca gaaataagaa atccagataa gacaatgttc gtccaagacg
    25081 ctattcgctt cgcacagcag cacggattca atatgcttta attttaaagt ttaggtgtca
    25141 agacctcttt ttagtgtgcc caaaatttag agaagtaatc aatcaactaa cttttatttt
    25201 tttcaaactt tcagtaaact gacctaaagc taactcaatc tgtctttgta gatgcttctg
    25261 ctatcagcta gaagttgatc tacttttgcc aagactgcct tctcatcaaa agttccaggt
    25321 tgatagttgg attgcaggga tggaatcttg tttttcaaag ccgcttcata tcccttagtt
    25381 tgaaccttga tgtagtgatt gtggtcgcca tgaggaatca caaaaccttc tgaatcttca
    25441 cttataattc gattggcatc aaaaccatga ccatcttctt cctcatgatg gacatgtagt
    25501 gacggattac ttaatacaga actagaagaa cttcctacct cttccgtgtt agagtgtgat
    25561 gggggattgt taagagatga cttaggaata tagtgatagt gatccccatg tcttactata
    25621 taagcatcac ctgtatctct gacaatatca ttagggttaa agacatatgt ggctgctaat
    25681 tcacctgccg acaagtcact ctcaggaatg aaatgatagt gaccaccatg tggtactatc
    25741 gtagattgaa atagaatatg agcaaattga taaggggatt ttaaagtaat ttctaacaat
    25801 gatttagaaa ctatgatgtg ctattctaaa ttcaactcac tatatataac catcatcggt
    25861 agtataacgt ccctgtaatt ttgctacaga tacttctgca ctagctcctt tatcgtcttt
    25921 accatgttct tgtttttggc gattgatttc atcttttatt cgtacatttt ctgcatgagc
    25981 ttgatcttta aggtaaacat aatactttcc atctacctta ataatatatc ctcccttaac
    26041 ctaactgacg atatcttgat ctttcggctg atagttgggg gctttcatta gtagctcttc
    26101 actaaagagc gcatccaaag gaactttacc attatagcag tgataatgat cgccatgaga
    26161 agttacataa ccttgatctg taatcttaat aacaatttgt tttgcttgaa ttccttcttt
    26221 ttgactaacc tagtctggag tcaaattttc agtcttctta gtgtctttat tactgtttac
    26281 atatgaaaca cgatttttat ctgtattggc ctgttagcta tgttggttca gagcataaac
    26341 acacagactt aaggaaagga taacaacaga tccagctgct atatatttct ttttaaattt
    26401 cataattacc tcatttctat aattatttat atgatgtctt cattattaaa tgattaaata
    26461 aattaattaa ccaattaatt aactagtaaa tattccacct ctttttaagt tgtatgtcaa
    26521 gaaattttat atattaataa taaaatgaaa ttctcccaaa gtcagagttt tatttctaac
    26581 ttttgagaga acttcatttt tgattcagac tttttctact gctattcctt acgctatgag
    26641 atcagataaa ttctttttta tcacttctcc acttggcaat cttaattcaa tcgttccatc
    26701 catattgaat ataacactat ctaaacctaa tccgtaacta gctgtaaatt tttctaattt
    26761 ttcttgtaca ggatctactg ctggagcttc ctctaatgct ggatctaaca tagggtcact
    26821 ccccacattc ccttctggat tcaacattcc attatccgtt gagttttctg gttttacagg
    26881 ttttttgttt ggtgcctctg gtaaagaatc tgctggttta ttttctgttg gttggttctc
    26941 aactgttcca gtagatactt ttccattttc agatggttta ttttcaccat ttccttgagg
    27001 tgcttctcct gtaaaatctg ccatattctt tttaatgact tctcccgatg gtaaatataa
    27061 ttcaattgtt ccgtccatat taaacaagac attttctagc ttcatcccat aactttcagc
    27121 aaattttgct actttttctt gtacaggatc cactgtagga acttcttcta acgttgaatt
    27181 actagtacta ttcccagttt cagaaagttt ttctttttct accttctcac tagtctttgg
    27241 ttcttctacc ttttcatcaa gttttgagtt ttcttgtgct ttattccttt taaattgtgg
    27301 tagaatactt ggtttatcag tttgattttc tttttccaag ataggtactt ccacaatata
    27361 agtcgattga ttgtccaaat aagcatttgc catgaaggtt acaggaattt tatttccggc
    27421 cgttctggtt gttccttggt ttaatttcgg aatcggtaat ttaatttcac caactttata
    27481 gttattttct aaataagcat ttccatgaaa ttcatcaaac actctgacta aagcatcagt
    27541 tcctttaggc actgcaaatt gagggttcac tcttaaataa gtatcccctg catggaaagg
    27601 atagaaaatc gtttgactgg ccattttgta agctaaagag gttggaactg taaatgtacc
    27661 atcataactt acttctggat aatcttttga agcgatagta tacttaaatg tttgtcctgg
    27721 taaataaggt tgatctaatt caaagtttgc aatattccct actccttctc caaatacttt
    27781 accagatact ttctccaata cttttccatc tggtgttatt aattttacta gcatattgat
    27841 acctaatttt ttctccaatt caggcggaaa actaaaagaa acgcgttttt gaccattggc
    27901 tagagtaaag ttttgattat taaacgtact attttttaac aaattaacaa cattcgttaa
    27961 ttcttctcca gtataaactt tattcccttc ttttttagca actccttctt cgggtttaaa
    28021 cagttcatag ttactgtgag aatgaccaat tccaaccggt ttatgttcat caatcggatc
    28081 tgcatgatgg tgatctccat gcggataaat aatcgcattt ttttctttat tcacgacaat
    28141 actttcacgt ttgacaccat attgtttcat aatgccagca attttttctt caattttttt
    28201 atctaaatct ttcatttctt tggcattact tggataatcc tgttcatgag atgacaaaga
    28261 atctaatcca ttatgactag ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg
    28321 ctcttctgtc aagtccttct tgaagaaata atgattgtgg tctccgtgac tcatgacaaa
    28381 acctgattca tcttcagaga taatacgatt agcatcaaat ccgtatccat cttcttcatg
    28441 tttctcatgt gaagttcctg gattgattgg aagagatgga gaaggtgttg ctagactatt
    28501 gtttggaaga gtcggttgcc caatttgatt tgattttgga atgtaatgga aatgatcacc
    28561 atgtcttaca atataagctg tagccgtttc ttcaacgata tcttttggat taaaaatata
    28621 accatcagat gctgaagaga gctccttact tgtcgttaaa gaagaaggat tgcttgaaag
    28681 actgcctaga ctagacacta cttcattagg ttttgcattt gtagaaactg tagaaccagt
    28741 tccactgata ggcaccattc tggcaatctt ttcttctaag gcagaaagct tgctgtaagg
    28801 aataaagtgg taatggtcgc catgcggaat cgcaactcca tttggtgtac gactgataat
    28861 cttagcaggg tcaaagacca ggccatctga ttcactgtaa cgttgggcgc taggtgaatc
    28921 atagagttcc ttcaaaagac tctggagatt ttcagattta tttgctggct tgctagttga
    28981 tccttttgct acagattgcg tgttattgtc actagctgtt gaagaatagc ttaactgact
    29041 cggttgcata ttttttccaa ccagatgtgc tttagctgct gctaattcac tagcagataa
    29101 atcgcttttg ggaatgtagt gatagtgacc tccatgagga acgatataag cattacccgt
    29161 atcttcgata atatcagctg gattaaagac ataaccatca tttgtcgtat atcgtccctg
    29221 agaccttgct acagcaacat tagagttaac cttctcatta tctttgacat gttcttgttt
    29281 ttgacgattg atttcatctt tagttcgaac attatcagca tgagctgcat ctttcaggta
    29341 gacataatat tttccatcga ccttgatgat ataaccaccc ttgacttcat tgacaatatc
    29401 agcgtcttta agttgatagt ttggatcctt catcaagagt tcttcactaa agagggcatc
    29461 ataaggaact ttcccattat agtaatgata gtggtcaccg tgtgacgtta catagccctg
    29521 atctgtaatt ttgattacaa tttgctcagc ctgaattcct tctttctggc taacctggtc
    29581 tggtgtcaag ttttcacttt tctgacttga ctggctgcca tccacataag agacacgatt
    29641 attgtcctta ttttcctgcg aacgatgctg gtttagtgca taggcacata gactcaagga
    29701 tacgataaca gctgatccag ctgctatata ttttttacta aatttcataa atccctcatt
    29761 tcaataaatg atgaagtttt ttctcaactt cttttacttt attaaatagt tttctaaacc
    29821 ggttatttaa ccatttaatt aaccagtaaa tagtttacta tttaaaatta cttttgtcaa
    29881 gtttctgtta cgtttttcta aaaaagagaa gtaacacgaa aggttacttc tccataaaat
    29941 atgctccatt cattttacta ctgtatcgga gccggttgac ttttttttaa caaagccaag
    30001 agactatcta cttctgctga aatagtgtta ttatctttcg ttccgagaag aagactactt
    30061 tttagaccag tcaatgtctc catagcattt tgtctaatac tagaatctgt tactttttct
    30121 agcaaggcct ccgcctctgc tatcttagcg ttaataacag aattctctac ttgaggaatt
    30181 tcagtctcat ctgtagtatc ttcagcttct tcctctgtct cttccgtatc agtgcttggt
    30241 ttataaagat tatctgctga aggatttaaa ccagcgtgat tctctttttc atcagattca
    30301 gggtgagttg gctcacttac ttcatcatgt cccttatctt catcaggttt actgtcttgg
    30361 tctgccttat tttta
//
LOCUS       DAVMMR010000025        28830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMMR010000025 DAVMMR010000000
VERSION     DAVMMR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.025"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            189..635
                     /gene="rplM"
                     /locus_tag="VYS74_001060"
     CDS             189..635
                     /gene="rplM"
                     /locus_tag="VYS74_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV3789113.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            655..1047
                     /gene="rpsI"
                     /locus_tag="VYS74_001061"
     CDS             655..1047
                     /gene="rpsI"
                     /locus_tag="VYS74_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV3789114.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(1309..1731)
                     /locus_tag="VYS74_001062"
     CDS             complement(1309..1731)
                     /locus_tag="VYS74_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3789115.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            1878..2054
                     /locus_tag="VYS74_001063"
     CDS             1878..2054
                     /locus_tag="VYS74_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789116.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2455..4668
                     /locus_tag="VYS74_001064"
     CDS             2455..4668
                     /locus_tag="VYS74_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV3789117.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4890..5366)
                     /locus_tag="VYS74_001065"
     CDS             complement(4890..5366)
                     /locus_tag="VYS74_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV3789118.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5589..8792
                     /locus_tag="VYS74_001066"
     CDS             5589..8792
                     /locus_tag="VYS74_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV3789119.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9172..9557
                     /gene="tnpA"
                     /locus_tag="VYS74_001067"
                     /pseudo
     CDS             9172..9557
                     /gene="tnpA"
                     /locus_tag="VYS74_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9936..10565)
                     /locus_tag="VYS74_001068"
     CDS             complement(9936..10565)
                     /locus_tag="VYS74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV3789120.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(10575..11576)
                     /locus_tag="VYS74_001069"
     CDS             complement(10575..11576)
                     /locus_tag="VYS74_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV3789121.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11607..12248)
                     /locus_tag="VYS74_001070"
     CDS             complement(11607..12248)
                     /locus_tag="VYS74_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV3789122.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12267..13082)
                     /locus_tag="VYS74_001071"
     CDS             complement(12267..13082)
                     /locus_tag="VYS74_001071"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV3789123.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGEANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            13353..13787
                     /locus_tag="VYS74_001072"
     CDS             13353..13787
                     /locus_tag="VYS74_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3789124.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            13799..14989
                     /locus_tag="VYS74_001073"
     CDS             13799..14989
                     /locus_tag="VYS74_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV3789125.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            15000..15491
                     /locus_tag="VYS74_001074"
     CDS             15000..15491
                     /locus_tag="VYS74_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3789126.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            15506..16285
                     /locus_tag="VYS74_001075"
     CDS             15506..16285
                     /locus_tag="VYS74_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3789127.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            16272..17090
                     /locus_tag="VYS74_001076"
     CDS             16272..17090
                     /locus_tag="VYS74_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3789128.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            17090..17383
                     /locus_tag="VYS74_001077"
     CDS             17090..17383
                     /locus_tag="VYS74_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3789129.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            17405..19306
                     /locus_tag="VYS74_001078"
     CDS             17405..19306
                     /locus_tag="VYS74_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV3789130.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            19366..20367
                     /locus_tag="VYS74_001079"
     CDS             19366..20367
                     /locus_tag="VYS74_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3789131.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(20477..20812)
                     /locus_tag="VYS74_001080"
     CDS             complement(20477..20812)
                     /locus_tag="VYS74_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789132.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(20823..21065)
                     /locus_tag="VYS74_001081"
     CDS             complement(20823..21065)
                     /locus_tag="VYS74_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789133.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(21081..21275)
                     /locus_tag="VYS74_001082"
     CDS             complement(21081..21275)
                     /locus_tag="VYS74_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789134.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            21441..22391
                     /gene="rsmH"
                     /locus_tag="VYS74_001083"
     CDS             21441..22391
                     /gene="rsmH"
                     /locus_tag="VYS74_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV3789135.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMEMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLRPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            22403..22720
                     /gene="ftsL"
                     /locus_tag="VYS74_001084"
     CDS             22403..22720
                     /gene="ftsL"
                     /locus_tag="VYS74_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV3789136.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            22724..24976
                     /gene="pbpX"
                     /locus_tag="VYS74_001085"
     CDS             22724..24976
                     /gene="pbpX"
                     /locus_tag="VYS74_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV3789137.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQISQQTVDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITKDFVWRDILYQSNYE
                     PGSAMKVMTLAASIDNNTFPGGEYFNSSELKIADVTIRDWDVNEGLTGGGMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     NYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KSLEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFEGSGTTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            24978..25958
                     /locus_tag="VYS74_001086"
     CDS             24978..25958
                     /locus_tag="VYS74_001086"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV3789138.1"
                     /translation="MFISISAGIVTFLLTVVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLSSLLTLAILYLM"
     gene            26566..28671
                     /locus_tag="VYS74_001087"
     CDS             26566..28671
                     /locus_tag="VYS74_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3789139.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGAAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYIIEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
BASE COUNT         8926 a         5085 c         6350 g         8469 t
ORIGIN      
        1 ttcagttact ttgagaagtt accgaaaaat atttttcata tctattgact tttaggggtg
       61 aaatttggta tgatagtagg cggtattgtt taccccattt gaaaggcccc ggaaccttcc
      121 aaatactttt cgatgggaag gaacacccat caccgtaaac aaaaatcgaa ctatatatag
      181 gagaaatcat gaacaaaaca acatttatgg ctaaaccagg ccaagttgaa cgtaaatggt
      241 acgtagttga cgcaactgat gtaccacttg gacgtctttc tgcagtagtt gctagcgtac
      301 ttcgcggaaa aaacaaacca acatttacac cacacactga tacaggtgac ttcgtgattg
      361 ttatcaatgc tgaaaaagtt aaattgactg gtaaaaaagc aactgataaa atctactaca
      421 ctcactcaaa ccacccaggt ggattgaaac aaatctctgc aggtgaactt cgttctaaaa
      481 atgcagtacg tttgattgag aaatcagtta aaggtatgct tccacacaat acacttggac
      541 gcgctcaagg tatgaagttg aaagtatttg ttggagctga gcacactcat gctgcacaac
      601 aaccagaagt tcttgacatt tcaggactta tctaaggaaa ggaacaaata aagtatgtca
      661 caagcacaat atgcaggtac tggacgtcgt aaaaacgctg ttgcacgcgt tcgccttgtt
      721 ccaggaactg gtaaaatcac tgttaacaaa aaagatgttg aagagtacat cccacacgct
      781 gaccttcgtc ttgtcatcaa ccaaccattc gcagttactt caactgtagg ttcatacgac
      841 gttttcgtta acgttgtagg tggtggatac gctggtcaat caggagctat ccgtcacggt
      901 atcgctcgtg cccttcttca agtagaccca gacttccgcg attcattgaa acgcgcagga
      961 cttcttacac gtgactcacg taaagttgaa cgtaagaaac caggtcttaa gaaagctcgt
     1021 aaagcatcac aatttagtaa acgttaattc gaaagaatta ctatacttat acagagcacc
     1081 tttcggggtg ttcttttttt atactttctt actaaattgg tgcaattgac acagttgttg
     1141 cgactttagt cgcttacaaa tgtggctgca acctgacaag gtcagttgcc tcaaaacgtt
     1201 aatcaataca attatatcaa cgtttcaaag cactcaagag tttacctcat gggtgctttt
     1261 tttatgtttt ttgaaaaagt tcacctcaaa gtttacctta ttttcacctt attgttttag
     1321 agactgtaag gcaaattcac cgacggccat agggactaca ttagaagtat tgacataaat
     1381 ctgtgttgta cctgtgtcgc tatgtattag agcttcagaa atggcttcta agctcattcc
     1441 tgcttgttta gcgagcgttg ctccggtatg acgtagtttg tgtggcgtag cgtgtgtaag
     1501 ctctgggtgt cgttttctga tagttttcat cttattattg agatagtcag aatgtaaagg
     1561 tttattgata ttgcctttgg tatctatgta agtaaagatg aattgttctg gattactgat
     1621 gataccaaac tttgccagtt catatttttg ttgttttttc catgaagtca gaagttgaag
     1681 taagtcatta gaaatagaga aaatgggaaa tgtttccaca ataaaacgca tattatcaga
     1741 tggttaatgc gttttatatt tgggtttgag gcttataaaa taggataagc gtactgaaaa
     1801 cttagatatg acaaacaaaa taaaattttt aaaagctaca gagaaacatg gtataatgta
     1861 tctaaaggag gtagcatatg tccttagata tagataaaga aaagatgaca atcatgggaa
     1921 tagcttttga aaaccgatcc gtttttaaga gtgtttggta tgctttaagt acaaatatga
     1981 ttgaaggatg gcgacctacg gttagtgatg ttgaaaaatt acgagatgaa gctcttgctc
     2041 tggggatgac ttaatgaagc gcaactgtta tgagtcttat gactatgttg atccggataa
     2101 tttatatacc gatccgggtt cttcagttct aagaaataag caggaaggaa aaaagcacgc
     2161 gagcttgaat atcgtatgat tgctagtaag agtttgaaat tatttatcaa tcctatctta
     2221 gttaatagtt taaaagtagt ttgtttcaca actgcataag cttagaaaag ttgaaaagtg
     2281 ataactcttt aataaactct tggtttgtta aaagatattt tgtccactac attttttcaa
     2341 ttgactatta tttgtcatag atataacaga tagaacattg cattcagaat aatgaaaaac
     2401 gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa caaattgaaa
     2461 atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt aaggttagaa
     2521 tactctcaaa ctggaagttt tgaggataga acgacacaac ttatctataa tagagatttt
     2581 ggccaagttt cgttagatta tatcgagaca tcaaacgtac tagatattat gacggactat
     2641 tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat agaattaaaa
     2701 ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga aacgttaaaa
     2761 ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga tggaattatt
     2821 agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga tgaacaatct
     2881 ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg gcatgattat
     2941 agaggagcaa tcagagactt ttaccatttg actggttcaa cacccttgtt gccaagatat
     3001 gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata cttggattta
     3061 atagacagat ttgcaacaga gaaaattcca ttatctatcg gtgtgttaga tatggattgg
     3121 catataactg acattccagc tcgttttgga agtggctgga caggatatag ttggaataga
     3181 aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa gttaaaactc
     3241 tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta tcctcaaatc
     3301 gcaaaacggt tggggttaaa tgtagaacta gaagaacctg ctatttttga tttttttaat
     3361 ccctctttta gggaagccta ctttaaagat gttcattatg agctagaaaa gcagggagta
     3421 gatttttggt ggattgactg gcaacagggg acacaaggca tgctagatcc actttggctt
     3481 ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt gattttatca
     3541 agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga tactattatc
     3601 agttggaatt ccttaagatt tcaaccctat tttacagcga cagcatctaa tatcggttat
     3661 agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga agagctacaa
     3721 actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag ttctagaagt
     3781 ccttttaata gtaaagaacc ttggtttttt tcagaaacaa catctaagat tatgaagaaa
     3841 taccttcgtt tgagacatca gatgattcct tatctatata ccatgaatgt aaagacacat
     3901 gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa tgatgagagc
     3961 tataatgttc caaaccaata cttttttgga acagaactga tggtggctcc cattgtagaa
     4021 aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga aggtgaatgg
     4081 tatgacttct tttcagagaa aaaatacaca ggtggtgtga agttaagtgt ttatagggac
     4141 atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag
     4201 ataggtatgg gtgttgattt acctgaggtt gtagattggt atgtattccc agggaaacaa
     4261 cattcttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag attatcaatc
     4321 gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat cgttccaagc
     4381 aatagaaaac atagaattca ttttaaagga acgaatgtgt ctataattga attgccaaat
     4441 aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt aaatgacgaa
     4501 gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag attgttaaat
     4561 caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca tcaggataag
     4621 gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt gaaataatta
     4681 gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc actggtcaca
     4741 ttgaaaatgt gaaataagca cctacaacat agactttaca acacttcatg tttcatatca
     4801 tggggtgttt ttctatttaa gtcaaagcca atcaaaaaat ctcactattc atcaggttaa
     4861 aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc ctcttcaatt
     4921 tgttttgggt ctgtttttga agaaaagcgt tcaaagactt gcccatcacg accaatgaga
     4981 aacttagcga aattccattc gattcgtttt cctagtgggc cagatttatg gtcttttaac
     5041 cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag
     5101 gtggtttgaa aatgtaggct acagaaggtg ttgatttcct ctgcgctgcc gggtgcttgt
     5161 cccataaact gattgcaagg gaaatccaat atttcgaagc cctgatcttg atagcgttca
     5221 tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc agtgttgaca
     5281 atcaagagaa ctttgccacg atagctatcc aagggagttg cttggttgtt ttggttcaaa
     5341 acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca ttgtaacagt
     5401 ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagcc ctcaatatga
     5461 tagaaatcgt ttacaaataa ctttaaaaat ggtatgattt aattaaaccg gtttactttg
     5521 ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat gtttattgga
     5581 gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca cttgttttat
     5641 caggattttt attaaaccat tatatgacag ttggagcgga agaaacgact acgaatacca
     5701 ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat ttagttgaaa
     5761 atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc aaagctcagg
     5821 ggtggtcagc ttgggtagac cagaagaata gttctgcaga tgcctcaact cgagtcattg
     5881 aggctaagga tggggctatc actatctcaa gccctgagaa attaagggca gcggttcacc
     5941 gtatggttcc tattgaagct aagaaaaagt ataaactgcg tttcaagatt aaaacagata
     6001 ataaagtcgg gattgccaag gttcgtatca ttgaggaaag tggtaaggac aagcgattgt
     6061 ggaattctgc aacgacgtca ggaacaaagg actggcagac cattgaagca gactatagcc
     6121 cgactttaga tgttgataaa atcaagctgg agttattcta tgaaacagga actgggactg
     6181 tttcctttaa ggatattgag ctggtagagg tagcagccca gctttctgag gattctcaaa
     6241 cagataaaca gcttgaggaa aagattgatt taccaattgg aaaaaaacat gttttttctc
     6301 ttgcggacta tacttataag gtagaaaatc ctgacgttgc ttcagtcaaa aatggaattt
     6361 tagaacctct taaggaaggg acaaccaatg tcattgtcag taaagatggc aaggaagtga
     6421 aaaagattcc tttgaagatt ctagcctctg ttaaggatgc atacacagcc cgtttggatg
     6481 actggaatgg catcatcgct gggaatcaat actatgattc taaaaatgaa cagatggcca
     6541 aattaaacca ggaattggaa ggaaaggtag ctgatagcct atctagtatt tcaagtcagg
     6601 cggaccgcac ctatttgtgg gaaaaatttt caaattataa aatgtctgca aatctgactg
     6661 ccacttatcg gaaattggag gagatggcca agcaagtgac caatccttct tctcgttatt
     6721 atcaagatga aactgtcgtt cgaacagtca gggattccat ggaatggatg cataaacatg
     6781 tctacaatcg tgaaaagagc attgttggga actggtggga ttatgaaatc ggtacacctc
     6841 gtgccatcaa caataccttg tctctgatga aagaatactt ctctgatgag gaaattaaaa
     6901 aatatacaga tgtgattgaa aaatttgtac cagatcccga acatttccga aagacgactg
     6961 ataacccatt taaggctcta ggtggaaact tagttgatat gggaagagta aaagtaatag
     7021 ctggtttact gcgtaaggat gatcaagaaa tttcttctac cattcgctcg attgagcaag
     7081 tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga tggatcctat atcgaccaca
     7141 ccaatgttgc ctatacgggt gcttatggga atgttttgat tgatggcctg tctcaactgt
     7201 tgccagtcat tcaaaagacc aagaatccaa tcgataaaga taaaatgcaa accatgtacc
     7261 actggattga taaatcgttt gctcctttgc tggtgaatgg agagctgatg gatatgagtc
     7321 gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt ggccgcagta gaagtactaa
     7381 gagggattca ccgaatagcg gatatgtctg aaggagaaac caaacaacgt ttgcagagtc
     7441 ttgtgaagac cattgttcaa tcggatagtt attatgatgt ctttaagaat ttgaagactt
     7501 ataaggatat cagtttgatg caatccttgt taagtgatgc aggagtcgca agtgttccaa
     7561 gaacaagtta cctatctgcc tttaacaaga tggataaaac agccatgtac aatgcagaga
     7621 aagggtttgg atttggcttg tcactctttt ccagtcgtac cttgaattac gaacacatga
     7681 acaaggaaaa taaacgtggt tggtatacga gtgatgggat gttctatctt tacaatggcg
     7741 atttgagtca ctatagcgat ggctactggc caacagttaa tccatataag atgcctggta
     7801 caacagagac ggatgctaag agagcggata gcgatacagg taaagtttta ccgtctgctt
     7861 tcgttggaac gagcaaacta gatgatgcca atgcgacagc aaccatggat ttcaccaact
     7921 ggaatcaaac attgactgct cataagagct ggtttatgct aaaggataag attgcctttt
     7981 taggaagcaa tatccaaaac acttcaacag atactgctgc aactacaatc gaccagagaa
     8041 aactggaatc aagtaatcca tataaagtct atgtcaatga taaagaagcc tcccttacag
     8101 aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt agaatcgtcc gattcgaaaa
     8161 agaatattgg ttactttttc tttaagaaga gttcaatcag catgagtaag gctttgcaaa
     8221 agggagcctg gaaggatatc aatgaaggac agtcagacaa ggaagttgaa aatgaatttc
     8281 ttacgattag tcaggctcat aagcaaaatg gagattctta tggctatatg ctcattccta
     8341 acgtggatcg tgccaccttc aatcaaatga taaaagagtt agaaagtagc ctcatcgaaa
     8401 ataacgaaac ccttcagtct gtttatgatg ccaaacaagg agtttggggc attgtgaaat
     8461 atgatgattc tgtctctact atttccaacc aattccaagt tttgaaacgt ggagtctata
     8521 ccattcgaaa agaaggggat gaatataaga ttgcctacta taatcctgaa acccaggaat
     8581 cagctccaga tcaggaagtc tttaaaaagc tagagcaagc agctcagcca caagtacaga
     8641 attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca ttcggatcaa aagaatctcc
     8701 ctcagacagg agaaggtcag tcaatcttgg caagtctagg gttcttgcta cttggggcgt
     8761 tttatctatt ccgtagagga aagaacaact aatttgttca tcatctagat gaattacatg
     8821 aaactgtgga aaatagaaaa aatccgaaga aggccaaata tcttcggatt tttcttttaa
     8881 aaaaggataa gttgatactt atccttttta cttcattatg tgactatagc gttttgaaac
     8941 tagaatagga catcataact gctaaaagat ttctataaat tcatttgatt ttcctaatca
     9001 atttgttcgt atcctatttc actccactat attagtccgt ctcgctccgt gaggtgcgag
     9061 acaaaacaac cacccgctat acgggtgcgc gtcgaaggtt ataccaaaaa aactccaaac
     9121 gcgatacaat aaaggtgttc aatccacttg taaagcgaaa ggagaaaaaa tatggcacaa
     9181 aaggctcata gtttatctca cacaaagtgg aggtgttcta tcacattgtg ttcaccccta
     9241 agtatagacg aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc
     9301 gcttgtgtag ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac
     9361 acatgttagt cagtattccg tcgaggctaa gtgtttcaag ttttatgggg tatttaaaag
     9421 gcaaaagtgc actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc
     9481 ggcatttctg ggcggaaggt tattatgtaa gtccagtagg gcttaatgaa gccacaatta
     9541 agaaatatag tcaagattaa agaaattatc cagtggatga tttcttcacg agtatgaaaa
     9601 tttgagaacg agtaaagtat gatatatagt aaaatgaaat aagaacagga caaaatcgat
     9661 caggacagtc aaatcgattt ttaacaatgt tttagaagta taggtgtact atttcagttt
     9721 caatctacta tagcactaga taaattgagt gtaaaagaat atgaggatcc ctttagggat
     9781 agtggtaagt aataccaaag tctcttaaag aggcaagtga cgagtcaaga gcaataaggc
     9841 ttgaacaacg tgaaagccag cgtctttagg cgctggctga tgatttgggc ttatagctct
     9901 gagataaacc acccgttaga cgggtagtta tgattttatc tgagtgtaat atactgttgg
     9961 gcaatctcgc tgatgcggtc aaagttgcct tgggaagcga gtttattgag ttcgccacca
    10021 attccaacgg catctgcacc agcagcgaac cattgaggga tgttgtttag gccgactcct
    10081 ccggttacca ttacggaaac ttgtgggatc ggtgccttga ctgcagagat atatgctgga
    10141 ctgagagtac tacctgggaa gagtttgatg atttcactac cggcttcaag tgcagtcgtg
    10201 atctctgtga gggtaataca gcctggaatg tacggtgtgc tgtagagatt gcacattttc
    10261 gcagtttcag catggaaaga tggagaaaca acgtaatttg ctccagctag aatggcatct
    10321 ctagcagtta cggcatcaag cacagtacct gcaccgatac aaacactctg atcgtcctga
    10381 tacaagtcta caagttcctt gatgatttgt cctgcatact gattggtata ggcgatttca
    10441 atagctttga taccgccctt gatacaagca atcgaggctt gtagtccttc ttcctttgta
    10501 tttcctcgaa tgacagcgac aattttctgt ttttttagtt caataatcgt atctgatttg
    10561 gtcatgtaat tctcctaacg aatgatatct tgtgcatttg ccagtaaatt ttcaatacta
    10621 gttgcggaag tggagagatg gtctcctgga agagtgcatt tgagagttgc gctcgcaact
    10681 gcaaagtcaa tggtagtttt tagggaggaa tgatggagta gttggtaaag ggcaccagat
    10741 ataaaggcat ccccgctacc aattcgttga tagactgcag tttttagttg gacagactct
    10801 tcaaatcgtt cttccagagc ataggcttga tagacatttt tgtcttgctc atcactagat
    10861 cggagggtat ggaaaatagc cttgaaacca taggcttctt ttaaaagtcg catgcgattt
    10921 tccacctctt ctaggctagc actgtctctt gggaacatct ctagattttg gtcatcaatc
    10981 atgagaggat caataccgaa gcaatagtca gtaaaacgtg caaacttaga aaattcatac
    11041 ttggcttcta ggactgaaat catctttgtt ctcagattga gatccattga aacgacaatt
    11101 cctcggcgct tggcttcttc caagagtagg agaaggatcg cacggacctc ttgaccgata
    11161 gctatggtga ttccactaaa atgaaaatgg ctaatcccct gaaagagaga atccatatct
    11221 agcatgtttg gccgaatctg gctgatactc gtatgcttac gatcgtagaa aacttcactt
    11281 tgacgacaac caaagccgtt ctccaaatag tagaggccga ttcgatcgcc aagccgacag
    11341 attgaactgg tatcgatttg gtgctgtttc aagaatgtga gaaaacgatc tccaatctcg
    11401 ttggcaggga gtgcggtaaa gactttcgtt gagataccca gggcttgcaa attacaagcg
    11461 atgttaattt ctgatccgcc aaaataagtc gaactggcaa catgatcgcc gatactggtg
    11521 gcatctaatg gtgagattcg aattaatggt tcgccaaaca gtaatatttt cgtcatccat
    11581 tagttccttt ctggtttaga atagctttac ttggctaata cttctttcaa ataagcagcg
    11641 acttcatcat cttggcagtt ttcaaagaag tattcttgga agtatttgcc agagatggtg
    11701 tctttgagga agtcttggtc gattgtttta aggatggtca tcaaatcatt gtgggtgatt
    11761 tgtttcacct cgtttaagat acgagcgttg cgttgttcag ggattacacg ttctcttggg
    11821 tagccaccgc ccatttcttc agcaaataag cgttcaaaca tcaatttgag ggtcagttct
    11881 gccccccagc caaatccttt ggcataaggg atagacaagg cgttaccacc attgatttga
    11941 gaataaaggt aagcgtcagt tgggtccact gctagaccac agacaacacc agggaagctg
    12001 tttaaagcaa gcatagcccc tacacccgta ccacagccgg taacaacaaa gtcaactgcc
    12061 tttgtattta aaaggatggc agccattagt ccgttctgca cataagttaa ttgactttct
    12121 ccttcttctc cacgcatacc atagttaaat aattggtagc cttttttatc tgtcgcttct
    12181 tttagactat cgtaaataat gtgattcttg ctagcttgac tattttcatt gattaatgcg
    12241 attttcataa gatcttcttt ctatttttac tcaggttgtt ttccgatgta ggctaagata
    12301 ccgccatcta catataggat gtggccattg acaaaattgc tggcatcact agcgagaaag
    12361 acagcagggc ccatcaaatc ttcaggattt ccccaacgtg cagcaggtgt ttttgcaatg
    12421 atgaactggt caaatgggtg gcgagaacca tcttcttgca actcacgaag aggtgctgtt
    12481 tgaggagtgg caatataacc cggtccaatt ccgttacatt ggatattggc ttcaccgtat
    12541 tcagacgcaa tgttgcgggt caacattttc aagccccctt tagcagcagc ataagcgcta
    12601 actgtttcac gtcccagttc gctcatcatc gaacaaatat tgataatctt tccatgccct
    12661 ttctttatca tagaaggaat aactgccttt gaaacgataa atggtgcgtt taagtcaata
    12721 tcgatgacct tacggaaatc agcggcgctc atttcgcaca ttggaactcg gcggataata
    12781 ccagcgttat taacgaggat gtcaatgaca ccaacctctt gttcgatttg cttgaccatg
    12841 gcttggatac cgtcctcgtc tgtcacgtca cagacatatc catgggcttc gatgccaact
    12901 tcacgataag cttcaatccc tttattgacc agatcttgat tgatatcgtt aaagacaata
    12961 gtagcgccgg cttcagcgta ggatttggca atagcaaatc caattccata agaagcgccg
    13021 gtgatgagag caatttttcc ttttaaagaa aactgctcaa ttgagaatga tgtatttgtc
    13081 atttgatact tcctttcaaa tttgttaaga cttctctata accaaattat attctagtaa
    13141 accggttttg tcaataaata aatattttta tttataaaaa gatttatttt ccttaaaaat
    13201 ggaatatttt gaaatttctg tctattagaa tgattaaaaa gataatgaaa gcgctaatat
    13261 ttttttaaaa aaactattga caagataaaa ataaaggatt acaatgggga atataaagta
    13321 aaccggtaaa cctaaacaag aaaggagaaa agatgaaaat tgtacttgta gggcatggac
    13381 attttgctac agggatttat agttctttac aattgattgc aggcaatcaa gaaaatgtgg
    13441 aggcaattga ctttgtggaa ggaatgtcag cagatgaact caagcaaaaa atcttacttg
    13501 caatttcaaa tgaagaagaa gttttaatcc taagtgatct cttgggagga tcgccattca
    13561 aggtttcttc taccataatg ggagaaaatc cagccaaaac aatgaatgtt ctttcgggtt
    13621 tgaacttagc catgttaatg gaagcagtct ttgctagaat ggctcatagc tttgatgagg
    13681 ttgttaataa atcagtagtg gcggcccagg gcggagtcgt aaatggtaaa gaattgtttt
    13741 caacggatgc agaggaagag gaagaagatt tcgaatcggg tatttaaagg gtaaaagaat
    13801 gataaaaaag gttacgattg aaaaaataaa atcgcctgag cgcttcttag aagtaccact
    13861 tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt attcggcagt tagaactcaa
    13921 ccttgactat ttcaaggaag atttcccgac gccagctacc tttgataatg tctatccaat
    13981 catggataac acggaatgga ccaatggttt ctggacagga gaactgtggt tggcttatga
    14041 atacagtcaa caggatgcat ttaaaaacat cgctcataaa aatgttcttt ctttcctgga
    14101 tcgtgtcaat aagagagtag aattggatca ccatgatctc ggcttcttgt acacaccgtc
    14161 ttgtatggct gaatataaga taaatggaga tggagaggct agagaagcaa ccttgaaagc
    14221 tgcagataag ttgattgaac gctatcaaga aaaaggtggt tttattcaag cttggggaga
    14281 cttgggcaag aaagagcatt accgtttgat tatcgactgc ttgctcaata tccaactctt
    14341 attctttgct tatcaagaaa caggcgatca aaaatactac gatattgcag aaagtcattt
    14401 ctatgcttca gctaataatg taatccgtga tgacgcttcg tccttccaca ccttctattt
    14461 tgatcctgag acaggtcaac cctttaaagg tgtaacgaga caagggtata gtgatgattc
    14521 atgctgggca cgtggtcaat catggggagt ctatggtatt cctttgactt atcgtcactt
    14581 aaaagacgag tcctgctttg acttgtttaa gggtgtgacc aattatttct tgaatcgtct
    14641 gccaaaagat catgtgtcct attgggattt gatttttaat gatggtagtg atcaatcacg
    14701 agattcttca gcaacagcta tcgccgtctg tgggattcat gaaatgctaa aacatcttcc
    14761 agaggtggat gctgacaaag atatttataa acatgctatg catgccatgc ttcgttcctt
    14821 gatcgaacat tatgcaaatg atcaatttac ccctggtggg acaagtctcc tccacggtgt
    14881 gtactcatgg cattcaggta aaggagtgga tgaaggcaat atctggggtg actactatta
    14941 cctagaagcc cttatccgtt tctacaaaga ctggaaccta tattggtagg aggagaaata
    15001 tgacaatgcc aaatattatt atgacccgta tcgatgaacg gttgattcat gggcaaggac
    15061 aactttgggt aaaataccta ggttgtaata cggtcattgt tgccaatgac gaagtaagca
    15121 cggacaagat gcaacaaact ctgatgaaaa cagttgtgcc agactcagtt gccatgcgtt
    15181 tcttcccttt gcaaaaggtg attgatatca ttcacaaggc taatcctgct caaacgatct
    15241 ttatcgttgt aaaggatgtg aaggatgctt taaccttggt agaaggtggt gtccctatca
    15301 aagaaatcaa tattgggaac attcacaatg ctactggtaa agagcaagtg acacgctcca
    15361 tcttcctggg tgaagaggac aaggcggccc tcaaggaatt gagccaaact catcaagtaa
    15421 catttaatac gaaaacaact ccaacaggaa atgatggagc tgttcaagtc aacattatgg
    15481 actatattta aaagaggaga tcgttatgtc gattaatgta tttcaagcga ttttaattgg
    15541 attatggaca gctttctgtt ttagtggaat gctgttagga atttacacca atagatgtat
    15601 tgttctgtca tttggtgtcg gaattattct aggtgatctg cctactgctc ttgcaatggg
    15661 agctattggt gaattggctt atatgggatt cggtgttggt gctggaggta ctgttccacc
    15721 aaacccaatc ggacctggta tctttggtac cttgatggct atcactagtg ctggtaaagt
    15781 cagtccagaa gcggctcttg ccctctctac tccgattgct gtggcgattc aattcttaca
    15841 aactttcgcc tacactgtac gtgctggtgc gcctgaaaca gcgatgaagc acttgaaaaa
    15901 ccataatttg aagaaattca agttcactct aaatgcaaca atttggttgt ttgcctttat
    15961 tggatttacc ttgggttgct tgggtgccct ttcaatggat accttgttga aactcgtaga
    16021 ctacattcca ccggtattgc ttacaggttt gacagttgct ggtaaaatgc tcccagctat
    16081 cggttttgcg atgatcttgt cagtgatggc taagaaagag ttgattccct ttgtcttgtt
    16141 gggatatgtt tgtgcagctt atctaaacat cccaacaatt ggtattgcaa ttgtaggtac
    16201 tatctttgct ttgattgaat tttataacaa gccaaaaaca gcggatcatg tggtagagga
    16261 ggaagcacac gatgactgga tctaacaaat taacaaaacg tgattatctt aaaacgtctt
    16321 tgcgggcatt cttttgtcaa aatggattta actatagtaa ctatcaaggg ttgggatatg
    16381 ccaatgtgat gtatcctgct ttgaaaaaac actatggaga ggatcaggaa ggtttctacc
    16441 aagccttgga agaaaactgt gaattctata ataccaaccc acacttcctg ccttttatta
    16501 ccagcttgca tcttgtaatg ttggaaaatg gccgtccggc aaaagaaaca cgtagcatca
    16561 agatggcctt gatgggacca ttggcaggta ttggggattc tctttctcaa ttctgtttag
    16621 ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga aggcttggtt gtcggtccaa
    16681 tcttgttctt ccttgcgatg aatacgattt taacagcgat taaattgtca actggtctgt
    16741 atggatacaa actaggaaca actgtgattg ataaactaag cgaacagatg gcaacgattt
    16801 ctcgtattgc caatattatc ggtgtaaccg taattgctgg tttggcagcg acatctgtta
    16861 agattatggt gccgattacc tttgctgcag gggaagttaa agcagacgct aaacaaagta
    16921 tcgtaagtat tcagggaatg cttgataagg ttgctccagc tcttctacca gccctattta
    16981 cacttttagt ttactacttg atcaaagaaa agaaatggac aacatataaa ctcgttattt
    17041 taacagttat catcggaatt atcggaagct ggcttaagat tatagcttaa tggatacaac
    17101 attgttttat ggaatagtga ttgtcttggc agtgagtccc cttttactgt caagctttca
    17161 ttctattcgt cagcaaaagt tgcttcgcaa acagatggag caacgacaag agtatttagc
    17221 ttctttaaca tctggtgatg aagtgttgtt attgtctgga attcatggaa aaatcatttc
    17281 tatccaagat gacttggtct ccttgcagat tgcaaaaggt gtggtcatct atgtagaaaa
    17341 ggaaagtgta atgggaaaga caaaagaact gctttttaag tagttctttt tctggtaata
    17401 gataatgaaa gaagaaagaa gacaattttt tgaaagagtc gatggaaacc aatgtcgtga
    17461 ttatatcttg tcccactgtt caaaagacta tgagaaagtc aagagttccc ttgaacgctt
    17521 gatagacaat cgttttatgt ttgatagtcc ttgggatatg gagccttgtt caaaaatcca
    17581 tcaaatccag ccgatggtat gggatcaagt atttgaagat gatccagaat ggtcttatat
    17641 gctcaatcga caagaatatc tcttgcagtt tatgataggg tatctggtag aaggagataa
    17701 gggctatatc caaaagtgca agttctttct atttgattgg attgagcagg tgagagaatt
    17761 ttctcctcaa tccttgatga ctagaacctt ggatacgggt attcgttcct ttacttggtt
    17821 gaaactactc ttgctcctct tgaaatttga cttgctagag gagaaagaac tagagaaaat
    17881 tttggtcagt ctagaaaagc agattgactt tatgaaaagc cactatcgcg ccaagtacac
    17941 ccttagtaac tgggggattt tacaaacaat tccgatgctt gctatctatt atttcttttc
    18001 agataagatg gacctagaag aagcttacca ttttgcttca gaggagttga aacagcaaat
    18061 tgagacacag attttaggag atggaagcca gtttgaacag tcgattctct atcatgtaga
    18121 ggtttataaa gccttgctgg atttgtgtct cttgcttcca gacttgcaag atagttaccg
    18181 agagttgctg gaaaagatgg cgacctatat tcaaatgatg acaggcttag atggacgaac
    18241 tttggctttt ggtgatagcg attctacaga aacgacagaa attttgagcc tgtctgctgt
    18301 ggttttgaac aaggaagacc ttcttaacgg tctggatgtt aaagtcgatt tgcttagcct
    18361 cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa tttgaaagga gagcttggca
    18421 gcctaagtcc atgatctttg aagactctgg acatgtctgc attaaggatg aacatcgtta
    18481 tctatttttc aaaaatggtc cgctaggaag tgcccatagc catagtgacg agaatagttt
    18541 ttgcttacag tatcaaggcc aacctatttt catagatgct gggcgttatt cttatcggga
    18601 gatatatgaa cgttatctct taaagagtgc ttggagtcat tcgacctgca ttgtagatgg
    18661 gaaagctccg gaaagaatca cgggatcctg ggaatatgaa tactatcctc actccctgtt
    18721 ttgtcaccat aaagaaaggg agggagtgca ttatattgag ggggcttatt ggtcagcaga
    18781 acctgatttg ccttatcttc acaagagaaa aatcctcatg ttggtagagg atgtctggct
    18841 cttggtagat gacatcaggt gtcaaggtca gcatgaggcg ttgactcagt ttatccttga
    18901 caaggatgtg acctatcaag atgggaaaat caatcagttg agactatgga gtgaagttga
    18961 ttttgatttg gaagatacca tcatttctcc taaatacaat gagcttgaaa gaagtagcaa
    19021 actcaccaag cgccaattct ttgagaatca gatgctggat tataccatca ttgcgcatga
    19081 gagttttgaa attatccgtc attctgtcta ccagacagat gatcgtgaag tggaaaatgc
    19141 tctggctttt gaagtgaaaa atgacgaaac agacaagctg attctgttat taagcgagga
    19201 tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca aaaatgcgtg gaaaatgtct
    19261 agtatatgat aaaataaatg agagaatgat tcgcttgcag tgctagaaat aggcattttg
    19321 aatagtgaat atgttataat aagtattagt aggaggtgtt ttagattgga gaagaaactg
    19381 accataaaag acattgcgga aatggctcag acctcgaaaa caaccgtgtc attttaccta
    19441 aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa agattgaaaa agttattcat
    19501 gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa actccaaacg aacaaaatta
    19561 atcggtgttt tgatcggtga tattaccaac agtttctcaa accaaattgt taagggaatt
    19621 gaggatatcg ccagccagaa tggctaccag gtaatgatag gaaatagtaa ttacagccaa
    19681 gagagtgagg accggtatat tgaaagcatg cttctcttgg gagtagacgg ctttattatt
    19741 cagccgacct ctaatttccg aaaatattct cgtatcatcg atgagaaaaa gaagaaaatg
    19801 gtcttttttg atagtcagct ctatgaacac cggactagct gggttaaaac caataactat
    19861 gatgccgttt atgacatgac ccagtcctgt atcgaaaaag gttatgaaca ttttctcttg
    19921 attacagcgg atacgagtcg tttgagtact cggattgagc gggcaagtgg ttttgtggat
    19981 gctttaacag atgctaatat gcgtcacgcc agtctaacca ttgaagataa gcatacgaat
    20041 ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc ccgatgaaaa aactctggta
    20101 tttatcccta actgttgggc cctacctcta gtctttaccg ttatcaaaga gttgaattat
    20161 aacttgccac aagttgggtt gattggtttt gacaatacgg agtggacttg cttttcttct
    20221 ccaagtgttt cgacgctggt tcagccctcc tttgaggaag gacaacaggc tacaaagatt
    20281 ttgattgatc agattgaagg ccgcaatcaa gaagaaaggc aacaagtctt ggattgtagt
    20341 gtgaattgga aagagtcgac tttctaaaat gaaggaaaat gacttgcaat ttctgttaag
    20401 aaataaaata atcccaccta gaacaagcta ggtgggatta tttgcctatg aaatgagaaa
    20461 ttatgggagc aagctcctaa atcaactgtt tttgatctac ttctttaact acttgataaa
    20521 agttatagaa gtaggccaaa cttgaaatga tggttacgac taggaatatt gaaaatttcc
    20581 attggacagg gttggttaaa agttgtggaa aggatatgag gagaaagaag agggctgcgt
    20641 tgaggacagg tatccgtttt gattgtattt tctcaagtcc tttattgagc gcaggaagaa
    20701 agaggagtag gagtagtaaa actgtatgag aaacagctcc tgaaataagg gcgaagaaaa
    20761 ggaaaatact gataaaaaca tgaatgatca gtagtctagc tagtgatttc ataaggcacc
    20821 tcctaatcct ggtctttttt agctcttgca atacgaagtg agtcgacaat atgtatcatc
    20881 actccgaaaa agaaagctcc cagtatagtt ttaaaaatat gttttgtatt tagaagagaa
    20941 ctgataaaat ttggattttc acttgttagg gtatcaatga gtggaattat aaaaaatatc
    21001 actgttccat aaatcgaacc tgctttcaga ccaggataac gtaactgttt cttttctttt
    21061 ttcatgagtt tgctcctttt ttaaaaatct tccttccaaa agagactgtt gaggtcagtt
    21121 tggaggctgc gagcgagatt gagacagagt tccagagttg gattgtactt gtcgttttca
    21181 atcatattga tggtctgtct cgagacaccg atatccttgg cgagctcgag ctgggaaatg
    21241 cccagttcct tgcgaaattc tttcacacga ttcatctggt ctcctttctg atttgtgtcg
    21301 tatatatttg actttattat agtcttttaa acataaagtg tcaagtattt ttgacatatt
    21361 ttttgaagaa atagtagtct ccttgtccta tttgtctgac aagtgcaagc tggtcggatt
    21421 tgtggtaaaa tagataagat atgacaaaag aatttcatca tgtaacggtc ttactccacg
    21481 aaacgattga tatgcttgac gtaaaacctg acggtatcta cgttgatgcg actttgggtg
    21541 gagcaggcca tagcgagtat ttattaagta aattaagtga aaaaggccat ctctatgcct
    21601 ttgaccagga tcagaatgcc attgacaatg cgcaaaaacg cttggcacct tacattgaga
    21661 agggaatggt gacctttatc aaggacaact tccgtcattt acaggcacgt ttgcgcgaag
    21721 ctggtgttca ggaaattgat ggaatttgtt atgacttggg agtgtctagt cctcaattag
    21781 accagcgtga gcgtggtttt tcttataaaa aggatgcgcc actggacatg cggatgaatc
    21841 aggatgctag tctgacagcc tatgaagtgg tgaacaatta tgactatcat gacttggttc
    21901 gtattttctt caagtatgga gaggacaaat tctctaaaca gattgcgcgt aagattgaac
    21961 aagctcggga agtcaagcca atcgagacta cgactgaatt ggcagaaatt atcaagtcgg
    22021 ccaaacctgc caaggagctc aagaagaagg gccatcctgc caaacagatc ttccaggcta
    22081 ttcggattga agttaacgat gagctggggg cggcagatga gtctatccag caggctatgg
    22141 agatgttggc tttggatggt agaatttcag tgattacctt tcattccttg gaagaccgct
    22201 tgaccaaaca gttattcaag gaagcttcaa cggttgaagt tcccaaaggt ttgcctttca
    22261 tcccagatga tctcaggccc aagatggaat tggtatcccg taagccaatc ttgccaagtg
    22321 cagaagaatt agaagccaat aaccgctcgc actcagccaa gttgcgcgtg gccagaaaaa
    22381 ttcacaagta agaggaaaaa agatggcaga aagaatggaa aaaacaggtc aaatactaca
    22441 gatgcaactt aaacggtttt cgcgtgtgga aaaagctttt tacttttcca ttgctgtaac
    22501 cactcttatt gtagcaatta gtattatttt tatgcagacc aagcttttgc aagtacagaa
    22561 tgatttgaca aaaatcaatg cgcagataga ggaaaagaag accgaattgg acgatgccaa
    22621 gcaagaggtc aatgaactat tacgtgcaga acgtttgaaa gaaattgcca attcacacga
    22681 tttgcaatta aacaatgaaa atattcgaat agcggagtaa gatatgaagt ggacaaaaag
    22741 agtaatccgt tatgcgacga aaaatcggaa atcgccggct gaaaacagac gcagagttgg
    22801 aaaaagtctg agtttattat ctgtctttgt ttttgccgtt tttttagtca attttgcggt
    22861 cattattggg acgggcactc gctttggaac agatttagcg aaggaagcta agaaggttca
    22921 tcaaaccacc cgtacagttc ctgccaaacg tgggactatt tatgaccgaa atggagtccc
    22981 gattgctgaa gatgcgacat cttataatgt ctatgcggtc attgatgaga actataagtc
    23041 agcaacgggt aagattcttt acgtagaaaa aacacaattt aacaaggttg cagaggtctt
    23101 tcataagtat ctggacatgg aagaatccta tgtaagagag caactctcgc aacctaatct
    23161 caagcaagtt tcctttggag caaagggcaa tgggattacc tatgccaata tgatgtctat
    23221 caaaaaagag ttggaaactg cagaggtcaa ggggattgat tttacaacca gtcccaatcg
    23281 tagttaccca aacggacaat ttgcttctag ttttatcgga ctagctcagc tccatgaaaa
    23341 tgaagatggc agcaagagct tgctgggaac ctctggaatg gagagttcct tgaacagtat
    23401 tcttgcaggg acagacggca ttattaccta tgaaaaggat cgtctgggaa atattgtccc
    23461 cggaacagaa caaatttccc aacaaacggt ggatggtaag gatgtttaca caaccatttc
    23521 cagccccctc cagtccttta tggaaaccca gatggatgct tttcaagaga aggtaaaagg
    23581 taagtacatg accgcgacct tggtcagtgc aaagactggt gaaatccttg ctaccaccca
    23641 acgaccgacc tttaatgcag atactaaaga aggaatcact aaggactttg tttggcgtga
    23701 tatcctttat caaagtaact atgaaccagg gtcagccatg aaggttatga cgttagctgc
    23761 ttctattgat aacaatacct tcccaggtgg agaatatttc aatagcagtg aattaaaaat
    23821 agcggatgtg acgatccgag attgggacgt taatgaaggt ttgaccggcg gtggtatgat
    23881 gaccttttct caaggatttg ctcactcaag taacgttggg atgaccctcc ttgagcaaaa
    23941 gatgggagat gctacctggc ttgattatct taatcgcttt aaatttggtg ttccgacccg
    24001 tttcggtttg acggatgagt atgctggtca gcttcctgcg gataatattg tcaacattgc
    24061 gcaaagctca tttggacaag ggatttcagt gacccagacg caaatgattc gtgcctttac
    24121 cgctattgct aatgacggtg tcatgctgga gcctaaattt attagtgcca tttatgatcc
    24181 aaatgatcaa actgctcgga aatctcaaaa agaaattgtg ggaaatcctg tttctaaaga
    24241 tgcagctagt ctaactcgga ctaacatggt tttggtaggg acggatccgg tttatggaac
    24301 catgtataac cacagcacag gcaagccaac tgtaactgtt cctgggcaaa atgtagccct
    24361 caagtctggt acggctcaga ttgctgacga gaaaaatggt ggttatctag tcgggttaac
    24421 taactatatt ttttcggctg tatcgatgag tccggctgaa aatcctgatt ttatcttgta
    24481 tgtgacggtc caacaacctg aacattattc aggtattcag ttgggagaat ttgccaatcc
    24541 tatcttggag cgggcttcag ctatgaaaga ctctctcaat cttcaaacaa cagctaaatc
    24601 tttggagcaa gtaagtcaac aaagtcctta tcctatgcct agtgtcaagg atatttcacc
    24661 tggtgattta gcagaagaat tgcgtcgcaa tcttgtacaa cctattgttg tgggaacagg
    24721 aacgaagatt aaaaacagtt ctgctgaaga agggaagaat cttgccccga atcagcaagt
    24781 cctgatctta tctgataaag cagaggaagt tccagacatg tatggttgga caaaggcgac
    24841 tgctgagacc tttgctaagt ggctcaatat agaacttgaa tttgaaggtt cgggcactac
    24901 tgtgcagaag caagatgttc gtgctaatac agctatcaag gacattaaaa aaattacatt
    24961 aactttagga gactaatatg tttatttcca tcagtgctgg aattgtgaca tttttactaa
    25021 ctgtagtagg aattccggcc tttatccaat tttatagaaa ggcgcaaatt acaggccagc
    25081 agatgcatga ggatgtcaaa cagcatcagg caaaagctgg gactcctaca atgggaggtt
    25141 tggttttctt aattacttct gttttggttg ctttcttttt tgccctattt agtagccaat
    25201 tcagcaataa tgtgggaatg attttgttca tcttggtctt gtatggcttg gtcggatttt
    25261 tagatgactt tctcaaggtc tttcgtaaaa tcaatgaggg gcttaatcct aagcaaaaat
    25321 tagctcttca gcttctaggt ggagttatct tctatctttt ctatgagcgc ggtggcgata
    25381 tcctgtctgt ctttggttat ccagttcatt tgggattttt ctatattttc ttcgctcttt
    25441 tctggctagt cggtttttca aacgcagtaa acttgacaga cggtgttgac ggtttagcta
    25501 gtatttccgt tgtgattagt ttgtctgcct atggagttat tgcctatgtg caaggtcaga
    25561 tggatattct tctagtgatt cttgccatga ttggtggttt gctcggtttc ttcatcttta
    25621 accataagcc tgccaaggtc tttatgggtg atgtgggaag tttggcccta ggtgggatgc
    25681 tggcagctat ctctatggct ctccaccaag aatggactct cttgattatc ggaattgtat
    25741 atgtttttga aacaacttct gttatgatgc aagtcagtta tttcaaactg acaggtggta
    25801 aacgtatttt ccgtatgacg cctgtacatc accattttga gcttggggga ttgtctggta
    25861 aaggaaatcc ttggagcgag tggaaggttg acttcttctt ttggggagtg ggacttctat
    25921 caagtctcct gaccctagca attttgtatt taatgtaaga atggcaccct gatgtttcag
    25981 ggtgtttttg tatttaaata cacaatgaaa atcaaagaac aaactagaaa gctaacttta
    26041 ggctgctcaa aatatagtag attgaaacta gaatagtaca catctgcttc taaaatattg
    26101 ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttaatatac
    26161 agttttgaga tggctgatag aactgacgaa gtcagctcaa aacacggttt tgaggttgta
    26221 gataaggaga agctgatgtg gtttgaagag gttttcgaag agtgtaaaaa atattggtca
    26281 gttaaagtca aaactcttga ccttttctga ccaatgtagt atattatgaa cgtaaggtaa
    26341 atgaaagcct tactagaaca cttatttaaa gtaaaataga aagaggtggg tctatgtttc
    26401 atctagaaat cttcagaagt aaagatagtc tactcctgct tgaaaaagaa aaaccagaaa
    26461 tagtacgtag ggtagcgatc tagctagtct aaatttttta aaacaatggt caaagatagt
    26521 caatatcagt aatcataact aagtaaacaa aaagaggtaa agaatatgaa taacaacttt
    26581 aataatttta ataacatgga tgatttattt aaccaattga tgggtggtat gcgaggatac
    26641 agttctgaaa atcgccgtta cttaattaat ggacgcgaag tcacacctga ggaatttgct
    26701 cactatcgtg cgactggtca attaccagga aatgcagaaa ctgatgtgca aatgccacaa
    26761 caggcatcag gtatgaaaca agacggtgtc cttgcaaaac taggtcgaaa cttaacagca
    26821 gaagcgcgtg agggcaagtt ggatcctgtt atcggacgaa acaaggaaat tcaagaaaca
    26881 tctgaaatcc tctcacgccg caccaagaac aatcctgttt tggtcggagc tgcaggtgtt
    26941 ggtaagacag cagttgtcga aggtctagcg caagccattg tgaacggaga tgttcctgct
    27001 gctatcaaga acaaggaaat tatttctatt gatatctcag gtcttgaggc tggtactcaa
    27061 taccgtggta gctttgaaga aaatgtccaa aacttagtca atgaagtgaa agaagcaggg
    27121 aatattatcc tcttctttga tgaaattcac caaattcttg gtgctggtag cactggtgga
    27181 gacagtggtt ctaaaggact tgcggatatt ctcaagccag ctctctctcg tggagaattg
    27241 acagtgattg gggcaacaac tcaagacgaa taccgtaaca ccatcttgaa gaatgctgct
    27301 cttgctcgtc gtttcaacga agtgaaggtc aatgctcctt cagcagagaa tacttttaaa
    27361 attcttcaag gcattcgtga cctctatcaa caacaccaca atgtcatctt gccagacgaa
    27421 gtcttgaaag cagcggtgga ttattctgtt caatacattc ctcaacgtag cttgccagat
    27481 aaggctattg accttgtcga tgtaacggct gctcacttgg cggctcaaca tccagtaaca
    27541 gatgtgcatg ctgttgaacg agaaatcgaa acggaaaaag acaagcaaga aaaagcagtt
    27601 gaagcagaag attttgaagc agctctaaac tataaaacac gcattgcaga attggaaaag
    27661 aaaatcgaaa accacacaga agatatgaaa gtgactgcaa gtgtcaacga tgtggctgaa
    27721 tctgtggaac gaatgacagg tatcccagta tcgcaaatgg gagcttcaga tatcgaacgt
    27781 ttgaaagata tggctcatcg cttgcaagac aaggtgattg gtcaagataa ggccgtagaa
    27841 gctgtagctc gtgctatccg tcgtaaccgt gctggttttg atgaaggaaa tcgcccaatc
    27901 ggcagcttcc tctttgtagg gtctactggg gttggtaaga cggagcttgc taagcaattg
    27961 gcactcgata tgtttggaac ccaggatgcg attatccgtt tggatatgtc tgaatacagt
    28021 gaccgcacag ctgtttctaa gctaattggt acaacagcag gctatgtggg ttatgatgac
    28081 aatagcaata ccttaacaga acgtgttcgt cgcaatccat actctatcat tctcttggat
    28141 gaaattgaaa aggctgaccc tcaagttatt acccttctcc tccaagttct agatgatggt
    28201 cgtttgacag atggtcaagg aaatacagta aacttcaaga acactgtcat tattgcgacc
    28261 tcaaatgctg gatttggcta tgaagccaac ttgacagaag atgcggataa accagaattg
    28321 atggaccgtt tgaaaccctt cttccgtcca gaattcctca accgctttaa tgcagtcatc
    28381 gagttctcac acttgactaa ggaagatctt tctaagattg tagatttgat gttggctgaa
    28441 gttaaccaaa ccttggctaa gaaagacatt gacttggtag ttagtcaagc ggctaaagat
    28501 tatatcatag aagaaggtta cgacgaagtc atgggggttc gtcctctccg tcgcgtggtt
    28561 gaacaagaaa ttcgtgataa ggtgacagac ttccacttgg atcatttaga tgctaaacat
    28621 ctggaagcag atatggaaga tggcgttttg gttattcgtg agaaagtcta agacagaatt
    28681 ttgaggataa aaaagaagga gccagctgaa aaaaactggt tccttttgag gtatgacggg
    28741 catgtcgtat agtagaagtg tattattcta gtttcaattt actatagtag ctcagaagtc
    28801 ggtacttaaa cgtgctatat caaaaccagt
//
LOCUS       DAVMMR010000026        25812 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMMR010000026 DAVMMR010000000
VERSION     DAVMMR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.026"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..4786
                     /locus_tag="VYS74_001088"
     CDS             <1..4786
                     /locus_tag="VYS74_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3789140.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(5023..5571)
                     /locus_tag="VYS74_001089"
     CDS             complement(5023..5571)
                     /locus_tag="VYS74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3789141.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5892..6380
                     /locus_tag="VYS74_001090"
     CDS             5892..6380
                     /locus_tag="VYS74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV3789142.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6377..7564
                     /locus_tag="VYS74_001091"
     CDS             6377..7564
                     /locus_tag="VYS74_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3789143.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7557..7781
                     /locus_tag="VYS74_001092"
     CDS             7557..7781
                     /locus_tag="VYS74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3789144.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            7938..9284
                     /gene="asnS"
                     /locus_tag="VYS74_001093"
     CDS             7938..9284
                     /gene="asnS"
                     /locus_tag="VYS74_001093"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV3789145.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9437..9727
                     /locus_tag="VYS74_001094"
     CDS             9437..9727
                     /locus_tag="VYS74_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV3789146.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9739..10209
                     /gene="ssbA"
                     /locus_tag="VYS74_001095"
     CDS             9739..10209
                     /gene="ssbA"
                     /locus_tag="VYS74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV3789147.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            10241..10480
                     /locus_tag="VYS74_001096"
     CDS             10241..10480
                     /locus_tag="VYS74_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV3789148.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10775..12175
                     /locus_tag="VYS74_001097"
     CDS             10775..12175
                     /locus_tag="VYS74_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV3789149.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12177..12536
                     /locus_tag="VYS74_001098"
     CDS             12177..12536
                     /locus_tag="VYS74_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV3789150.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12590..13339
                     /locus_tag="VYS74_001099"
     CDS             12590..13339
                     /locus_tag="VYS74_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV3789151.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13329..13628
                     /locus_tag="VYS74_001100"
     CDS             13329..13628
                     /locus_tag="VYS74_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV3789152.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13699..14634
                     /locus_tag="VYS74_001101"
     CDS             13699..14634
                     /locus_tag="VYS74_001101"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV3789153.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14721..15233
                     /locus_tag="VYS74_001102"
     CDS             14721..15233
                     /locus_tag="VYS74_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV3789154.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15258..15866
                     /locus_tag="VYS74_001103"
     CDS             15258..15866
                     /locus_tag="VYS74_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV3789155.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            15967..16149
                     /locus_tag="VYS74_001104"
     CDS             15967..16149
                     /locus_tag="VYS74_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3789156.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(16195..17640)
                     /locus_tag="VYS74_001105"
     CDS             complement(16195..17640)
                     /locus_tag="VYS74_001105"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV3789157.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            17746..19368
                     /locus_tag="VYS74_001106"
     CDS             17746..19368
                     /locus_tag="VYS74_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3789158.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19571..21529
                     /locus_tag="VYS74_001107"
     CDS             19571..21529
                     /locus_tag="VYS74_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3789159.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21701..21934
                     /locus_tag="VYS74_001108"
     CDS             21701..21934
                     /locus_tag="VYS74_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV3789160.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            21940..23382
                     /locus_tag="VYS74_001109"
     CDS             21940..23382
                     /locus_tag="VYS74_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789161.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            23540..24634
                     /locus_tag="VYS74_001110"
     CDS             23540..24634
                     /locus_tag="VYS74_001110"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3789162.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24642..25808
                     /locus_tag="VYS74_001111"
     CDS             24642..25808
                     /locus_tag="VYS74_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3789163.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
BASE COUNT         8085 a         4736 c         5828 g         7163 t
ORIGIN      
        1 ccaaggtgaa cgcggtttaa ctggagctaa aggtgaaaaa ggggaccaag gtgaacgcgg
       61 tctcactgga gcccaaggtg aaaaaggaga ccgaggcgaa cgtggtttaa ctggagctaa
      121 aggtgaaaaa ggagcccaag gtcaggcagg tcgtgacggt gtaactccaa ccgtaaccgt
      181 taaagataat aaaaatgacg gcactcatac tatcactatt aacgacggta gaggtaatgt
      241 tacaagtact gttgtaagag atggtttcga tggcgcaagt ccattagttg cgactcaacg
      301 aaatgacgca gataagacaa caactgttat cttctattac gataaaaatg gaaacaatga
      361 attagatgct tctgataaga aattaaaaga agttgtaatt gcagatggtg ctaaaggtga
      421 aaaaggggac aaaggtgaac aaggtcttca agggcgtgat ggtgaacaag gaccaaaagg
      481 agaagatggg aaaaccccaa cagttaaagt aactgatggt caagatggaa cgcatacaat
      541 tacaattaac gatggtaaag gtggtataac tactacagta gtaagagatg gatttgatgg
      601 tgcaagccct cttgtttcta ctcatagaaa tgaagcagat aaaacaacaa ctgttatttt
      661 ctattatgat ctaaatgata ataatcaatt tgatgaagga gatacaaaac ttaaagaagt
      721 tgttatcgca gatggaaaac aaggaccaaa aggtgacaaa ggtgataacg gaaaagatgg
      781 attcacacca gaagtaacag ttacagataa caataacgga acacacacca tcacaatcac
      841 acaaccagac aacagaccat cattaacaac aatcgttaaa aacggtgaag atggaaaaac
      901 accaaaagtc aaagcagaac gagatgatgc gaagaaacaa acaacattaa cattctacat
      961 tgacaaagat ggagatggaa gttacacagc aggaaaagac gagttagttc aaacaacagt
     1021 agttaaagac ggacaagacg gagctgcagg agcatctgga cgtgatggaa aagaagtatt
     1081 aaacggaaaa gtagacccaa caacagaagg taaagatgga gacacattcg taaatacaca
     1141 aacaggagat gtattcgtta agaaaggtaa cacttgggaa ccagcaggaa acatcaaagg
     1201 accgaaaggt gacaaaggtg cagatggtgc caagggtgaa aaaggagccc aaggagaacg
     1261 cggcctgact ggagcgcaag gtgtcaaggg tgaaaaggga gatcagggtg aacgcggtct
     1321 aactggatct aaaggtgaaa aaggagacca aggtgaacgc ggtttaactg gagcgcaagg
     1381 tgccaaaggt gacaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa
     1441 aggcgcagat ggacaaagag gaccagcggg accacaagga ccaaaaggag aacaaggtaa
     1501 tccaggaact ccaggtaaag atggaaaatc tctaattgct gttaaaaatg gaactgaaac
     1561 aaaagtctat gtagaggatc cggctagacc aggacaacca cttaatccaa accaaccatt
     1621 agcaactatc actgatggaa gaaacggtac taatggacaa tcaccaacaa ttacagcgac
     1681 acgaagtgtg caaaatggca aaaatggtgt attagtaaca attacaccag taggaggtcg
     1741 tccacaaaca acatttgtag aggatggaca aaagggtgct gatgggaaaa ccccaacagt
     1801 aacaataact gaggggcaaa acggcacaca tacattaacg gttcataatc caggaagtcc
     1861 agatgtgaca actacgatcc gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga
     1921 tgtattaaac ggaaaagtaa atccacaacc aaaccaaggt aaaaatggag ataaatatat
     1981 taataccgaa accggtgatg tctatgttaa aaacaatgga aactgggata aagaaggcaa
     2041 catcaaaggc cctaaagggg acaaaggtgc agatggtgct aaaggcgaaa aaggagacca
     2101 aggcgaacgc ggcctaactg gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg
     2161 tgatggacgt gacggtaaag acgtgttgaa cggcaaagct aacccagaag cacatcaagg
     2221 taaagacggc gataaatacg ttaatacaga aacaggcgac gtcttcgtta agaataacgg
     2281 caactgggat aaagagggca acatcaaagg ccctaagggt gacaaaggtg aacgcggaga
     2341 agatggtaag actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat
     2401 tacattcact gttccaggta aagatccagt tacagtaaat gttaaggacg gagaaaatgg
     2461 tctgaacggt aaaactccaa aagttgattt acttcgtgtc caaggaaaaa acggaaatcc
     2521 atctcataca attgtgacat tctatacaga tgaaaacaat gacggcaaat atacaccagg
     2581 aactgatgaa cttctaggtt cagaaatgat taaagatggt gctaaaggcg cggacggacg
     2641 agatggtaaa tcattgctta ctgtcaagga tggtaaagaa actaaagttt accaagaaga
     2701 tccagctaac ccaggacaac cattaaatcc agaaaaacca cttgcggtaa ttagagatgg
     2761 agtagatgga aaatcaccta cagttacagc tgttcgtaaa gatgaagcag ggcataaagg
     2821 tgtagaaatc actgttgata accatgatgg ttcacaacca actacagtct ttgttcaaga
     2881 tggtgctaag ggagaaactg gtgcaaccgg tcaggatgga caaactccta caatcactac
     2941 tcaacgtgga caagatggcc aaagcactgt tgtaactatc acaacatcag gtaaagatcc
     3001 agtaaccttc actgtaaaag atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa
     3061 agtagaagat attacttcac cttcaagaat tagacgcgat acagatgctg ctgcaactcc
     3121 aacgcgtaac ggtatccgtg ttacagttta tgatgatgtt aatgacaatg gggtatacga
     3181 cgaaggtgtc gataaagtat taaatagtaa agatatttat aacggtatag atggacgtga
     3241 tggttcagct ccaactatta ctacaaaaga taatggagat ggaactcaca ctatcacagt
     3301 tcaaaaccca gatggttctg aatcaacaac agttgttaaa gatggtaaag acggtaaaac
     3361 tgcaaatatc actacaacag aaaacccaga tggaagccac acaattacgg tgacaaatcc
     3421 agatggttca actaaagaaa ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt
     3481 tgaagtaacg gataacaacg atggaactca tactgttaaa gttacagatg gagacggcaa
     3541 tgttaccaac gctatcatca aagatggtaa agacggtaaa gctgcaacag caacaactac
     3601 tgaaaatcca gatggaagcc acacagtaac aatcactaac ccagacggaa ctaagaatga
     3661 gtttgttgtt aagaatggac gtgacggtgt tgacggacgt actccaaccg catctgttcg
     3721 tgataatgga gacggaagtc atacaatcgt tattacaaat ccagaaggtg tgacaactga
     3781 aaccacagtt cgtgatggta aatcaccaaa agtgactata actgatgaac aaaatggaac
     3841 tcataagatc tctgttctaa atggtgacgg aacaactaca acaactacag ttcgtgatgg
     3901 tagagaacca aaacttgaag ttattgataa caacgatggt tcacacacta ttaaagtgac
     3961 aggtgctgat ggtaaaggaa cgacaactac aatctttgat ggtaaatcac caaaagcgaa
     4021 catcgttgat aacggagatg gaactcatac attaacaatc gtagattctg atggtcgtga
     4081 atacaaatct attatcaaag atggtaaaga cggcaaagat agcgtttcac caactgtaac
     4141 tgttaaaaat aataacgatg gaactcacgt tgttacaatc actaatccag atggaagtaa
     4201 gacagaaatg gtgattaaag acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga
     4261 taatggtgat ggtagtcata caatcacaat catcaattct gatggaactg tgacaaaaac
     4321 agttattaaa gatggtagag atggacgtga tggtcgagac ggcaaagacg gtaaagacgg
     4381 taaagatgga aaatgtggat gccaagacaa accagtaaca ccatcaaatg acaaaccagt
     4441 tcctccaaca ccaaatgtgc cgacaccaga agtaccggtt aaacctgtgc cagcgcaacc
     4501 aacaccaaat gtaccgacac cagaagtgcc agtacaacca actccagctg ttccaacacc
     4561 agaagtaccg gttaaaccag taccagcggt tccagaacaa ccagtagtac caacaccggc
     4621 tcaaccagca actccagtaa atgctaaccc agtagcacca actacaggta aagaaaaccg
     4681 tggggacaaa ttacctgaaa ctggaagcca atctgattat atctctgttc ttttaggtag
     4741 cggtattcta ttgagcctat atgtaggacg aagaaaagaa gcttaatcag ccaaaagcag
     4801 tcactaattt ctcgaaagaa gatgataagt gatagatctc gaatgaaaga atattcttag
     4861 aatgattttc ctatgatgga aaagactgct aaaaaccttg agtggtaacg ctcaaggttt
     4921 ttttggatgg ctgatgtttg tctgaaaatc ttgattgaga aataaaaagc caccctatta
     4981 ggtggctata attgatttag actttctaaa attttctatt ttttactgaa tcgcttcgac
     5041 caagtcttga gcttgttttt caagtgagtt taggactgtt tcttcaagaa ccaattttcc
     5101 gtctgcccag gcagagtcat taacacgtgc agcagtgaaa tcaccaacgc cttgtgtacg
     5161 gataaatggc aagaggtctt tgtagatagc gaaaagttga tcgtgccctg cattggctac
     5221 agatgatacg gtgacaaact tgtcttgaag ggcagaaacg ccacgcgtat cagacaagtc
     5281 aagggcacga gatagccagt caagcaagtt ttttactgta ccagggatag agaagttgta
     5341 gactggagag aaaatccaga tagcatccgc aacgagaact gcttcacgag cagcagctac
     5401 agctggatgt gttggaactt ccaaatcttg gctgaagaga ggaagggctg aataatcaag
     5461 gtagctaact tccgctttac cagcaagtgc tttctcagct tcgagcgcca tttggtggtt
     5521 gaaagaacct tgacgtagtg atccgacgat aaataatact tttttagaca tgatgtgtct
     5581 cctttttctt caaattttga gaactctctc gttgttataa aatacattac aacaaaacaa
     5641 aagaacttgc aagaaatttg ctcagagttt atttgaaaat tgtaaaattt cggaaactct
     5701 agtcatttgt gactgaaaaa gtaagaactg tttcactttc aaaatcggtt ttgtttgaag
     5761 ggagaaatag aattggttgc tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg
     5821 tttgaagaga ttttcgaaga gtattcttat attcacttgt aataaagctt aatagatggt
     5881 aaaatagacg agtgaaaaaa agacaaaaca aagcaaaaaa taatctactg tggcagtatg
     5941 gtctagggat gacgattttg tttgtggtta tcagtgcttc ctttctgtat atggtttctc
     6001 ttagcatgaa accctatcaa acagctaaaa gtgaaggaga aaaattagct cagcagtatg
     6061 caggattaga gcaggccgat caggttgatt tatacaatgg cttggaatct tattacagcg
     6121 ttcttggtcg taataaacag caagaagcac ttgctgttct gattggaaaa gatgatcata
     6181 agatttacgt ttatcagcta aatcagggtg tttcacaaga aaaagcagaa acggtttcta
     6241 aggaaaaggg agctggcgaa attgacaaga ttatctttgg tcgttatcaa gataagccaa
     6301 tctgggaagt caagtcagga tctgattttt atctagtaga ttttgaaaca ggagcattgg
     6361 tcaacaagga gggcctatga aattatccaa ccgtgtttta gaaatggaag aaagtgtgac
     6421 tttggctgct ggagcccgtg ccaaagcact gaaggctgag ggtagagata ttttgtctct
     6481 aaccttgggt gagccagatt tcactactcc caaaaatatc caagatgccg ccattgcatc
     6541 gattcgagat gggcgtgctt ctttttatac agtaacctca ggtctgccag agcttaaggc
     6601 ggcggtcaat agctactttg agcgctttta cggctattct gtagcgtcaa atcaagtgac
     6661 agtcgctgcg ggagccaaat attctctcta taccttcttt atggctgtgg tcaatccagg
     6721 tgatgaagtc atcatcccaa ccccatactg ggttagctat ggagatcagg tcaagatggc
     6781 agaaggcgtt cccgtctttg tttctgctaa ggaagacaat cactttaagg tgaccgtaga
     6841 gcagttagaa gcagctcgca ctgacaagac caaggttttg gtgctgaatt cgccatctaa
     6901 tcccacaggt atgatttaca cccgtgagga actcttggca attggaaact gggctgtaga
     6961 aaatgatatt ctcatcctag cagacgatat ctatggccgc ttggtttata atggtcatga
     7021 gttcacaccg atttctagcc tatcggaagc gattcgcaag caaacagtgg tcatcaatgg
     7081 tgtgtctaaa acctatgcca tgactggttg gcggattggt tatgccgttg gagaagcaga
     7141 cattatcgct gccatgtcca agattgcagg tcaaacaact tcgaatccgt cagcagtagc
     7201 ccaatatgca gcagttgagg ctctatcagg tgagcaagat actgtagaaa gcatgcgtca
     7261 ggcctttgag gaacgtctta ataccatcta tcccctcctt gcagaggtgc caggatttga
     7321 agtggtcaaa ccgcaagggg ccttctatct cttcccaaat gtcaaaaagg ccatggagat
     7381 gaaaggctac acggatgtga cagactttac aactgctatc ttagaagaag ccgaagtggc
     7441 cttggtaaca ggagctggat ttggagcacc agaaaatgtc cgcctcagct atgcgacaga
     7501 cctagacacg cttaaagaag cagtcgaacg cttgaaagca tttatgggta gtgagaatga
     7561 ttgatcattt tgagattaag gtaaaggatt tacaaatttc agaaggattt tataggagtt
     7621 ttctcgctcc tttggactat aaattggctt ttaagactag ttctctaatt agttttctat
     7681 ccccgaacag ccctcatcct ggtggtgatt tttggctgac tcagggaaca caagatcctg
     7741 ttcattttgc ttttttagca gaaaataagg aagaagttta ggcttgttat gaggctggct
     7801 tagaggcagg tgggcgagat aatggggctc ctggttatcg aagtgagcgt ccgatttact
     7861 atgctgcttt tatgattgac ctggatggga acaatataga ggtggtttgc cataaagaat
     7921 aaaaaagaaa aggaaaaata atgacaaaac gtgtaacgat tattgacgta aaagactatg
     7981 ttggtcagga agtgacgatt ggcgcttggg ttgccaataa atcaggaaaa gggaaaatcg
     8041 ctttcttaca attgcgtgat ggaacagcct tctttcaagg tgtggctttt aaaccaaact
     8101 ttgtcgaaaa atttggtgaa gaagtgggac ttgagaagtt tgatgttatc aaacgcttga
     8161 gccaagaaac gtctgtttat gtgacaggta ttgtcaaaga ggacgaacgt tctaaatttg
     8221 gctatgagtt ggacatcaca gacatcgaag tgatcggtga atctcaagac tacccaatca
     8281 caccaaaaga acacggaaca gactttttga tggataaccg tcacttgtgg ctacgctctc
     8341 gtaagcaagt agctgtgttg caaatccgta acgctattat ctatgcaact tatgagttct
     8401 ttgacaagaa cggttttatg aagtttgaca gcccaattct ttcaggaaat gcggcagaag
     8461 attctacaga actctttgaa actgactact tcggaacgcc agcctacttg agccaatcag
     8521 gtcagcttta cctagaagca ggggctatgg ctcttggtcg tgtctttgac tttggtccag
     8581 ttttccgtgc tgaaaaatca aaaacacgcc gtcacttgac tgagttctgg atgatggatg
     8641 ctgagtactc atacttgaca catgatgagt cgcttgactt gcaagaagct tatgtgaaag
     8701 ctcttctaca aggtgttctt gaccgcgcgc ctcaagcctt ggaaaccttg gaacgtgata
     8761 cagaactctt gaaacgctac attgcagagc cattcaaacg tatcacttac gatcaagcca
     8821 ttgacctctt gcaagagcat gaaaatgatg aagatgctga ctacgagcat cttgagcatg
     8881 gtgatgactt tgggtcacca cacgaaactt ggatttcaaa ccactttggt gtgccaacat
     8941 ttgtcatgaa ctatccagta gccatcaagg ccttctacat gaaaccagtt cctggaaatc
     9001 cagagcgcgt gctttgtgca gacttgcttg ctccagaagg ctatggagaa attatcggtg
     9061 ggtctatgcg tgaggaagat tacgatgccc ttgtcgctaa gatggatgaa cttggcatgg
     9121 atcgtacaga atatgaattc taccttgacc ttcgtaaata cggtacagtt ccacacggag
     9181 gatttggtat cggtatcgaa cgtatggtaa cctttgcagc aggaacaaaa catatccgtg
     9241 aagctattcc attcccacgt atgttgcacc gtatcaaacc ataagaacag gaaaacgccc
     9301 atgtggcgtt ttttcgaatt ctagtgtttt tacttgcgta aaaaaatttt ttctagtata
     9361 atagtttatt gtgagcgaac ctcacttacc ccttgcaaag tcttggggtc attagaccaa
     9421 aaggaggaac atatcaatgg ctaaatacga aattctttat atcattcgtc caaacattga
     9481 agaagaagct aaaaacgctt tggtagcacg ttttgactct attttgactg acaacggtgc
     9541 aactgttgtt gaatcaaaaa cttgggaaaa acgtcgtctt gcatacgaaa tccaagattt
     9601 ccgtgaagga ctttaccaca tcgttaacgt tgaagcaaat gacgatgcag ctcttaaaga
     9661 gtttgaccgt ctttcaaaaa tcaacgctga cattcttcgt cacatgatcg tcaaaattga
     9721 cgcgtaagaa ggtaaactat gattaacaat gttgtacttg tagggcgtat gacacgtgac
     9781 gctgagttgc gttatacccc atcaaatgta gcagttgcga cttttactct tgcagtaaac
     9841 cgtacattta agagtcaaaa tggtgaacgt gaggctgatt ttatcaatgt cgttatgtgg
     9901 cgccaacagg ctgaaaatct tgctaactgg gctaaaaaag gctcacttat cggggtgaca
     9961 ggtcgtatcc agactcgtag ttacgataac cagcaaggac aacgtgtcta cgtgacagag
    10021 gtcgtggctg agaatttcca aatgttggaa agccgtagtg tgcgtgaggg tcacacaggt
    10081 ggagcttact ctgcaccaac tgcaaactat tcagcaccta caaattcagt actagacttt
    10141 tcacgtaatg aaaatccatt tggagcaaca aacccattgg atatttcaga tgatgattta
    10201 ccattctaat ggacaactaa acttataaag gagaaaaaac atggctcaac aacgtcgtgg
    10261 cggattcaaa cgccgtaaaa aagttgatta catcgcagca aacaaaattg aatatgttga
    10321 ttacaaagat actgagcttc ttagccgttt cgtttcagaa cgtgggaaaa tccttcctcg
    10381 tcgtgtaaca ggaacttcag ctaaaaacca acgtaaagta acaacagcta tcaaacgcgc
    10441 tcgcgtaatg gctttgatgc ctttcgtaaa cgaagattaa agaaaagacc cggttgggtc
    10501 tctttttcat gagcttgaaa acaagaaagc gaattaagac cccaatgggt cttttttcac
    10561 aaactttgaa atgagagagc gagttaggct cagtggctcc ctttttacga gtttgaaaac
    10621 aagagaacac attaaaagca cgatggagct ttttcataaa tatgtctttt ttctcatcca
    10681 agaaattaaa ataaaaatga taaacttttt gaaaataggg gatttgccta ctttatgata
    10741 taatggaaag agttagattg aaagaggtag tgagatggac gcgaaattaa gatacaaggc
    10801 aaagaagatc aagattgtct tttttgatat tgatgataca ttgcggaatt caaagacagg
    10861 gtttattcca actacaattc ccactgtttt taaacagttg cgtgaaaaag gaattttaac
    10921 aggaatcgcc tctggacgtg gcatttttgg tgttgttcca gagattcgtg atctcaagcc
    10981 tgactttttt gtaactttga atggggctta tatcgaagat aaaaaaggtc aggtcattta
    11041 tcagcatcag attgaaaagt cagatgttga ggagtatatc tcttgggcta agcaagaagg
    11101 aattgagtat ggcttggttg ggagtcatga tgccaagttg tcgactcgca ccgatatgat
    11161 gagtgaagct atcaatccaa tttatcccga cttagatgta gatcccgatt tccatgaaaa
    11221 agaagatatc tatcagatgt ggacttttga agataaggga gatgacttgc acttgcctga
    11281 cagtctctca gacaaacttc gcatggttcg ttggcatcaa cattcgtctg atattgtgcc
    11341 gatttcaggc tccaaagcga cgggggtgga aaaagttgtg gaacaccttg gcttgaaacc
    11401 agagaaggtc atggtttttg gagatggtct caacgacttg gaactctttg attatgctgg
    11461 aatcagcgtt gccatgggaa tttctcatga taaaatcaaa gaaaaagcag attatattac
    11521 aaaaacatta gaagaagatg gcatttttga tgccttagaa gtatttggta tggtagaaaa
    11581 agaattgcat tttccacaag tagacattga aacagtagaa ggtcctcttg cgactattaa
    11641 gaccaatcac ggagacttac gtatcaagct tttccctgaa catgctccta aaacagtggc
    11701 taactttgta tctctttcaa aagatggcta ctatgatggt gtcattttcc accgtattat
    11761 caaggacttt atgatccaag gtggaaaccc aactggaact ggtatgggtg gcgagtcaat
    11821 ctacggcgaa tcttttgagg atgaattctc agaagagctt tacaatatcc gtggtgctct
    11881 ttccatggca aatgctggtc caaataccaa cggcagccag ttctttatcg tgcaaaacca
    11941 acacctacct tattctaaga aagaaattac tcgtggtggt tggccagaac cgattgcaga
    12001 aatctatgcc aatcaaggtg ggacacctca cctagaccgt cgtcacacgg tttttggtca
    12061 gttagctgat gaagcatctt acgctgtctt ggatgccatt gctgctgttg agacaggagc
    12121 tatggacaag ccagttgaag atgttgtaat tgaaactatt gaaatcgagg actaagatga
    12181 aaatcggtga taagctaaag gggcgtatta cagggattca gccctacggt gcctttgttg
    12241 agttagagac gggtgatacg gggctgattc acatttcaga aattcggaca ggatttattg
    12301 aaaatattca tgaaaccttg aaagtcgatg aagaggttca agttcaggta gtggatttag
    12361 atgaatttac ggggaaagcc agtctttcta tccgcacttt ggaggaagaa aagtaccagt
    12421 ttcctagacg gagacgcttt tcaagcgacc gctttaacta cggttttgct ccctttcgac
    12481 gaatgctacc aatctggact ggagaagccc tgcaccattt aaaaaagaag aagtagttta
    12541 gagttttata ttttttgtaa tgttaatata aatttgctat aataggacca tggaagaaga
    12601 acaattatta aaatcaggag agcgcattaa ccagctcttt tcgacagata tcaaaatcat
    12661 tcaaaataga gaggttttta gctattcggt ggatagtgtt ctcttatcac gatttccacg
    12721 ttttcctaag aaggggttga ttgtggattt ctgtgctggg aatggagcag tggggctttt
    12781 tgctagcact cgtactcaag cacagatatt gtctgttgag attcaggagc gtttggcgga
    12841 tatggctgaa cgctctgtcc gtttgaatgg attagaggag caaatgcagg tcatctgcga
    12901 tgatttgaaa aatatgcctg ctcacattca ggggagtaag gtggatatga ttttgtgtaa
    12961 tccaccctat ttcaaggtgg atccttattc taatctgaac gagagtgaac attatctctt
    13021 ggctcgacac gaaatcacga ctaatttgga agaaatctgt catagtgccc agagtattct
    13081 caagtctaat gggcgtttgg ccatggttca tcgtcctgat cgacttctgg atattttgga
    13141 tacgttaaaa cgacataatc tagcccctaa gcgcctgcag tttgtttatc caaaaagaga
    13201 aaaggaagcc aatatgcttt tgattgaggc gatcaaggat ggctcaacaa gtggctttaa
    13261 ggtcttacct cctttgattg tccacaatga tgatggctct tatacgcccg aaatcgaaga
    13321 gatttattat ggatcataag gcttatatgt acgttctgga gtgtcgtgat ggatcctact
    13381 atataggcta tacgactgat atgagaagac gcctcgctat ccacaatagt gggaagggag
    13441 ccaaatatac acgagcacgc ttgccagtca aacttatcta tgctcaaggt tttgccagta
    13501 aggaagaagc catgtcggct gaagctcttc tcaagcgtaa gaagaggcca cagaaggaag
    13561 aatttttatc tgaaaatcaa gatagaaatt tactcagtta ttttgaagaa tcgtggggag
    13621 tcctttgact cctcttacct ttgtcaaaaa gcgaaaaaaa tgctacaata aagaaaataa
    13681 acaaaatgag gtattatcat gtccaagatt ctagtatttg gtcaccaaaa tccagactca
    13741 gatgccatcg gatcatctgt agcttttgcc taccttgcaa aagaagctta cggtttggat
    13801 acggaagctg ttgcccttgg aactccaaat gaagaaacag cctttgtctt gaactatttt
    13861 ggtgtggaag caccacgtgt tatcacttct gccaaagcag agggggcaga gcaagttatc
    13921 ttgactgacc acaatgaatt ccaacaatct gtatcagata tcgctgaagt agaagtttac
    13981 ggtgttgtag accaccaccg tgtggctaac tttgaaactg caagcccact ttacatgcgt
    14041 ttggagccag ttggatcagc gtcttcaatc gtttaccgta tgttcaaaga acatggtgta
    14101 gctgtgccta aagagattgc aggtttgatg ctttcaggtt tgatttcaga tacccttctt
    14161 ttgaaatcac caacaacaca cccaacagat aaaatcattg ctcctgaatt ggctgaattg
    14221 gctggtgtga acttggaaga atatggtttg gcaatgttga aagctggtac caacttggct
    14281 agcaaatctg ctgaagaatt gattgacatc gatgctaaga cttttgaact caacggaaat
    14341 aatgtccgtg ttgcccaagt gaacacagtt gacatcgctg aagttttgga acgccaagca
    14401 gaaattgaag ctgcaatgca agctgccaac gaatcaaacg gctactctga ctttgtcttg
    14461 atgattacag atatcgtcaa ctcaaactca gaaatattgg ctcttggtgc caatatggac
    14521 aaggtcgaag cggctttcaa tttcaaactt gaaaacaatc atgccttcct tgctggtgcc
    14581 gtttcacgta agaaacaagt ggtacctcaa ttaactgaaa gctttaatgc gtaagatttt
    14641 gggtgtcagc tcaaaatcgg aaagtctagt ttgccttata tcgaaaggag tttcggctcc
    14701 tttttttcta ggagtgaagt atgttagaaa atggcgattt gatttttgtg agagatgggt
    14761 cagacatggg acaggccatc cagacttcca caggtaacta tagccatgtt gccatttatt
    14821 tggatgggat gatttatcat gctagtggac aggctggtgt tgtctgtcaa gaaccggcag
    14881 acttctttga gtccaatcat ttgtacgacc tctatgttta cccagaaatg gatatccagt
    14941 cggtgaagga aagagcttgc agacatcttg gagcacccta caatgcttct ttctatccag
    15001 atgcagctgg tttctactgc tcccagtata tagcagaaat cctacctatt tttgaaacta
    15061 ttcctatgaa atttggagat gaggaacagg agattagtga tttttggagg gagtattaca
    15121 tagaactagg tctgcctgtt cctctgaacc aagctgggac caatcctagt cagttggcag
    15181 catcgcctct gttacaatgt aaagaaagga atcttcatga ttcagatttt taatccatct
    15241 cgtttgacga gacagccatt tttggagaat tgatccgcta tctggaccag tatgaggatg
    15301 tgattctacg ggaaattaag gctcaatttc cagatgttgc agttgataaa ctcatggaag
    15361 agtatataaa ggcaagcttg attctacgtg aaaataagcg ctattacctt aattttccta
    15421 cgcttgaatc acttgatagt cttgaactgg atcaagagat ttttgtcaga gaagctagtc
    15481 cggtctatca agccttgttg gagcagagtt ttgagacgga attgcgcaat caaatcaatg
    15541 cagctatttt agttgaaaag acggactttg cgcgcattaa aatgaccctg tccaattatt
    15601 tttacaaggt caaacagcag tatcctttga cagaaaaaca gcaggagctc tatgacattt
    15661 taggagatgt taatcctgag tatgccctca agtatatgac ggcttttttg ttgaaatttc
    15721 tcaaaaaaga ccagcttatg cagaaatgcc gtgatatctt tgtggacagt ttggttgtct
    15781 taggctatat tgtgcaaaat gaagatggaa agtatgagtt ggctatcgat tttgataagg
    15841 agagattaac tttctactta gcgtgatttc ttgtttctga gtacattgtt tgactttcct
    15901 tagtattcgg tataaactat atgtaaccgg taacacatat cggaataaac taaaggagac
    15961 aatcatatgt cacttgaaaa caaattggaa caagcaacag gcgctgtcaa agaaggtttt
    16021 ggtaaagtta ctggagacag caagacagaa cttgaaggag ctgttgaaaa aacagttgct
    16081 aaggcaaaag acgttgtaga agacgcaaaa ggtgctgtag aaggtttgaa aaacgttttt
    16141 actaaataat aggaaaaaat caagggtttc attttccctt gattttttct attcttataa
    16201 ataattttct gcgacggctg tatctcctgg gtaggattct ttcttgccct ggatgatttg
    16261 gtaacaatcg actcccttac ccgcaataat aactgcatct aattcgtgat ttgtgatagc
    16321 cattgccgcc ttgatggctt cttggcgatc cgcaatcttt tcaacaggat gattgatgta
    16381 gctactaatt tcatctgcaa tggccattgg gtcttcatag ttagggtcat cagcagtcag
    16441 aaagacttga atctcagggt gttgattgag gaggaggcca aagtccttac gacgactttc
    16501 tcccttgttt cctgttgatc ccagaaccag agcaatcttt ccggtttgat gagtttccac
    16561 cacattgatg agttttttca gactatcccc attgtgggca tagtcgatga agaccttggc
    16621 tccatttttc tgagtgagga cttccatacg accaggaacg cgggttgcag cgatgccttt
    16681 tttgatgtcc tcaagacttg ctccgagacg gagacaagca agtccagcag caactgcatt
    16741 ttcttggttg aagttgccaa tgagttggat atcataatct ccagcgagtt tacccgtagc
    16801 tgaaaagcta aaggctttgg aattctcgat ttggttatca aattggctac catagaaatc
    16861 atggtcttga tcttcaacct gttctttcaa gactgagaag tggtccatgt cactgttaat
    16921 gatgactgct cggctatttt ccatcaagag acgcttgtgg tagaaatagt cttcaaagct
    16981 agggtgttca atcgggccga tatggtctgg gctgatattg aggaaaactc ccacatcaaa
    17041 ggttagacca tagacacgtt tgaccagata ggattgactg gagacttcca tgatgaggtg
    17101 ggtacggtca ttttgcacag cctgattcat catgtcaaag aggtcaatac tctcaggggt
    17161 tgtcaacgct gacttaaaga aagtctcgcc atcaagagtt gtgttcatgg tcgacaacat
    17221 agcaggtcta tgcccttgag ataagatgtt ataggcgaaa taggttgctg ttgtcttacc
    17281 cttagtacca gtaaaggcaa ggagtttgag tttctcttgt ggattaccat agaactccat
    17341 ggcaatcaaa ctcatggctt tctttatatc gttcacaatg atgacaggga tatcgacttc
    17401 gtagtccttt tcagctacat accaagctaa accttgtgtt atagcagaaa gaaggtattc
    17461 ttttttaaag gcagcgcctt ttgcaaaaaa aagagtgtct tctgttactt ttcggctgtc
    17521 gtagctgatg ctatcaaaaa caactttgct gtagttgtag tggtaatgac cttggtcaat
    17581 aatttcgcga aaaaggccat ctttctttaa aatatctaat acggtttcaa tcttaatcat
    17641 actttctatt gtaaaccgaa agtcgcaaat ttacaagtaa caaggaaaag tttataatgg
    17701 aagataagga gtttttccta gttatcaaaa ttgaatgagg aatctatgtc gcacgaaaac
    17761 aatcaccagc aggcccagat gttacggggg actgcttggc taacggctag taactttatc
    17821 agtcgcctac tcggggctgt ttacattatc ccttggtaca tctggatggg ggcttatgca
    17881 gctaaggcaa atggtctctt taccatgggt tacaatatct atgcttggtt cttgttggtt
    17941 tcaacagcgg ggattccagt tgcggtggcc aagcaagttg ccaagtataa taccatgcga
    18001 gaagaagagc atagctttgc cctgattcgg agcttcttag gctttatgac aggactaggc
    18061 ctggtttttg ctttagtctt gtatgtcttt gctccttggc tagcagactt gtctggcgtg
    18121 ggcaaagact tgatcccaat catgcaaagc ttggcttggg gagtcttgat tttcccgtct
    18181 atgagtgtta tccgaggatt tttccaaggg atgaataacc tcaaacccta tgccatgagc
    18241 caaattgctg agcaggtcat tcgtgttatc tggatgctcc tagcaacctt tatcattatg
    18301 aagctcggtt caggagatta tctagcagcc gttacccaat caacctttgc tgcctttgtc
    18361 ggtatggtag ccagttttgc agtcttgatt tatttccttg cccaagaagg ttcactcaaa
    18421 agagtctttg aaacaggaga taagattaac agtaagcgtc tcttggttga taccattaag
    18481 gaagccattc cttttatcct gacagggtct gccatccagc tcttccagat tttggatcag
    18541 ctgaccttta tcaatagtat gagctggttt accaactaca gcaatgagga cttggttgtc
    18601 atgttttctt atttctcagc caatcctaat aaaatcacga tgattttgat ttctgtaggg
    18661 gtttcgattg ggagtgttgg tttgccactt ttgacggaaa actatgtcaa gggggacttg
    18721 aaagcagctt ctcgtctggt tcaggacagt ctcaccctac tctttatgtt cttgctacca
    18781 gcaacggttg gagtggttat ggtaggagaa cctctttata cggtcttcta tggtaagcca
    18841 gatagtttgg ctctgggctt atttgtcttt gcagttttgc agtctattat tttaggcttg
    18901 tacatggtct tgtctccaat gcttcaggcc atgttccgca accgcaaggc cgttctctat
    18961 tttatctatg gttctattgc caagctagtc ttgcaactac ctaccatcgc cctcttccac
    19021 agttatggtc ctttgatttc aacaaccatt gctctcatca ttcctaacgt cttgatgtat
    19081 cgggatattt gtaaagtaac tggtgtcaag cgcaaggtga ttttgaagcg aaccatttta
    19141 atcagtttgc tgaccctagt catgtttctg ttaataggaa ccatccagtg gctgttagga
    19201 tttttcttcc aaccaagtgg acgtttgtgg agcttctttt atgtagctct tgtcggtgcc
    19261 atggggggtg gactttatat ggttatgagt ctgcgtacct atttattaga taaggtaata
    19321 ggaaaagccc aagcagatcg cctgcgagca aaatttaagc tttcgtaagg tagcatttat
    19381 aaataaaagg aataagttga acaatctttt aggagaactt gaaacttatt ccttttttag
    19441 cgtaaaaata gctaaaatta tctttcaaaa atagaaaatg aggttttttt cgattttttg
    19501 gaaaaataat tgactaaaca aaccaaacgt tgtataataa aacaaatatt taaagcagga
    19561 ggttctggaa atgaaaaagt ctaagagcaa atatctaacc ttggcaggtc ttgtcctggg
    19621 tacaggagtt ttattgagcg cgtgtggaaa ttctagcacg gcgtcaaaaa cctacaacta
    19681 tgtttattca agtgatccat ctagcttgaa ctatctagca gaaaaccgcg cagcaacatc
    19741 cgatattgtt gcaaatttgg tagacgggtt attagaaaat gaccaatatg ggaatattat
    19801 tccatcatta gcagaggatt ggactgtttc tcaggacggt ttgacctata cctacaaact
    19861 tcgtaaggat gccaagtggt ttacttctga gggagaagaa tatgcgcctg taactgccca
    19921 ggattttgtg acaggtttgc aatatgcagc tgataaaaaa tcagaagcct tgtatctagt
    19981 gcaggactct gttgctggtt tggatgacta tatcactggt aaaacaagcg acttttcaac
    20041 tgtcggtgtc aaggcacttg atgaccaaac ggttcaatat actttggtta aaccagaact
    20101 ttactggaat tcaaaaacac ttgcaacgat actttttcct gttaatgcag atttcctgaa
    20161 atcaaaaggg gatgattttg ggaaggcgga tccatctagt attttgtaca atggaccttt
    20221 cttgatgaaa gcacttgtct caaaatctgc tattgaatat aagaaaaacc ctaattactg
    20281 ggatgctaag aatgtctttg tagacgatgt gaaattgacc tactatgatg gtagcgatca
    20341 agaatcactg gaacgtaatt ttacagctgg tgcttatact acggctcgtc tttttcctaa
    20401 cagctccagc tatgaaggga ttaaagaaaa atacaaaaac aatatcatct atagtatgca
    20461 aaattcaact tcatatttct ttaattttaa cctagatagg aagtcttaca attatacttc
    20521 taaaacaagt gacattgaaa agaaatcgac tcaggaagca gttctcaata aaaacttccg
    20581 tcaggctatc aattttgctt ttgacagaac atcttatggg gctcagtctg aagggaaaga
    20641 aggtgcaaca aagattttgc gtaacctagt ggttcctcca aactttgtca gtatcaaggg
    20701 aaaagacttt ggtgaagttg tagcctctaa gatggtcaac tatggtaagg aatggcaagg
    20761 tatcaacttt gcggatggtc aagaccctta ctacaatcct gagaaagcca aggctaagtt
    20821 tgcggaagct aagaaagaac tcgaagcaaa gggtgttcaa ttcccaatcc acttggataa
    20881 gactgtggaa gtaacagata aagtaggcat acaaggagtt agttctatca aacaatcaat
    20941 tgaatctgtt ttaggttctg ataatgtagt gattgacatt cagcaattaa catcagatga
    21001 gtttgacagt tcaggctact ttgctcaaac agctgctcag aaagactatg atttatatca
    21061 tggcggttgg ggacctgatt atcaagaccc gtcaacctat ctcgatattt ttaatactaa
    21121 tagtggagga gttctacaaa atcttggact agagcctgga gaagccaatg acaaggctaa
    21181 ggaagttggg ctggatgtct atactcaaat gttggaagaa gctaataaag agcaagatcc
    21241 agccaaacgt tatgagaaat atgctgatat tcaagcttgg ttgattgata gttctttagt
    21301 tcttccaagt gtttcgcgtg ggggaacacc atcattgaga agaaccgtac catttgctgc
    21361 tgcctatggt ttaaccggta caaaaggggt tgaatcatat aaatacctca aagtacaaga
    21421 taagattgtc acaacagacg aatatgcaaa agccagagaa aaatggttga aagaaaaaga
    21481 agaatccaat aaaaaagccc aagaagaatt ggcaaaacat gtcaaataaa atctaattgt
    21541 agataagttt gtgtaagaag tctttgtgct taaaaacgca taatatcagg tatataaaat
    21601 cctagatgtt atgcgttttg ttataggaag atttattaga ttttatcctc aaattgagtt
    21661 attcccgact ttatccgttg aaatattata aggagtattt atgaaattaa aattattaag
    21721 agtagatact aaggtgatta tggggagttt cttccttgtt ctgtctagtc tacttgcttt
    21781 gttgcttccc cttatcttaa aaggtttaat agatgggagt tctattgaaa atataggttc
    21841 caaagtattt caatcgtttt tgatttttat tggtcaagcc ttgttttctt ctattggtta
    21901 ctatctgttt agtcaatcgg gtgaaaaaag atagcaaaaa tcaggaaaaa agtgatagag
    21961 gggttgattt atgcagagaa atccttcttt gataagagcc aaagtgggga gttgacttct
    22021 gccattgtca atgacacgag tgtcattcgt gagtttttaa ttacgacttt cccaaatatt
    22081 attctgagtt tagttatggt acttggttcc attgtagtct tatttagtct tgattggaat
    22141 ctttctctac ttttattcat cactcttcct tgtatgatgt ttattatctt gcccctttcc
    22201 aatatcagtg aaaagtatag tcgtcgttta caggaggaaa tcggattttt aacagggcaa
    22261 ttgactgaaa agattcaaga acatgaatta attaagacta accaagcaga gaaaagtgta
    22321 caggatgttt tggataattg tattgaaagg gtacaaaaca attcactgaa atcagatagg
    22381 attacttctt ttgagacccc gtttgctctc ttatttatct ttgcgactat agctgtgatg
    22441 ctaacctatg ggggttatcg ggtcagcgca ggatatatat ctgtgggaac cttggtttcg
    22501 tttttgattt acctctttca attacttaat cctattagta atatagctaa ttttgtaact
    22561 gtttattcta ggagcaaggg atcttcagtt gcactggaga acttgcttgc agttcctaaa
    22621 gaaaaatttg agggaggaaa atcggtatca ggacgagggt tgaattttaa ccatgtctat
    22681 tttggttatg atgaaaatcg acctgtctta aaggatatta tttgttcaat tttcaagggg
    22741 caaaaaattg cttttgttgg accatctgga tcaggaaaat caacgattgt gcgtttgtta
    22801 gagcggtttt ataaaccgct ttcaggagat attctaatgg agcaatcaag tatatatgat
    22861 tttaacttaa aagaatggag aagtaaaatc gcttgggttt cacaaaataa tgcagtctta
    22921 tctggcagta ttcgtgacaa tctttgtctc ggtttgaatc gcttagtaac tgatgatgaa
    22981 ttgatgaaag tgctagactt agtatcacta ggtgatgaga ttcgctccat gaaagaggga
    23041 ctagatactg aagttggtga acgcggacga ctcttgtcag gggggcaaag ccaaagactt
    23101 caaatagcta gagcctactt aaaagatgct gaaattctta tatttgatga agccactgct
    23161 aatcttgatg cggattctga gtatgcgatt atcagtagcc tctattctgt attaaaggag
    23221 aagacggttg tgattatagc gcatcgtttg tcaacggtaa aagatgtgga ttgtattttc
    23281 ttcttagagg aggggaaaat cactggctca ggaactcata aggaactact ggaaaatcat
    23341 gagcgttatg ctcgttttgt gcaggagcaa atgatagagt gaaggtgtct tttgagattc
    23401 accattttat agtctattaa agggagcagg aaaaactccc tttttatata gtttgaaact
    23461 ataactagct cttgaaaaca agaaaacgaa ggatacaaaa taagtggtac aatagttact
    23521 atagatttgg aggaattgta tgagcaagga attacacatt aacacaattt tagcacaagc
    23581 agggatcaag tctgatgagg caacaggtgc tttggtgaca ccacttcatt tttcaacgac
    23641 ctatcagcat ccagagtttg gtcgatctac tgggtttgac tatacgcgca ctaaaaatcc
    23701 aactcgtagt aaggctgagg aagtcttggc ggctattgag tcagcagact atgccctagc
    23761 gactagctca gggatgtcag ctattgtact ggcctttagc gtctttccag taggaagtaa
    23821 ggtcttggca gtgcgtgatc tttacggtgg ttcttttcgc tggtttaacc aagtggagca
    23881 ggaaggtcat ttccatttta cctatgccaa tacagaagaa gagttgattg ccgagttaga
    23941 aaaggatgtg gatgttctct atatcgaaac cccaaccaat cccttgatgt tggaatttga
    24001 tatcgaaaaa ctagcaaaat tggctcatgc taagggttcc aaagtggtgg tggacaatac
    24061 cttctatagc cctatttacc aacgtccgat tgaagatgga gcagatatcg ttctccattc
    24121 agcaaccaag tatctagcag gccacaatga tgtcttggct ggagtggttg tgaccaatag
    24181 tttagaacta tacgagaagc ttttttacaa tcttaataca acaggggcag tcttgtctcc
    24241 atttgacagc taccagttgc ttcgtggtct caagaccttg tctcttcgta tggagcgctc
    24301 aacagctaac gcccaagaag tggttgcctt tttgaaggat tctccagcag ttaaggaagt
    24361 tctctacact ggtcgtggag gcatgatttc ctttaaagta gccgatgaaa cacgcattcc
    24421 tcatattttg aacagtctca aggtcttctc ttttgcggaa agtttgggcg gagtggaaag
    24481 tcttattact tatccaacga ctcaaactca tgctgatatt ccagcagaag tacgccattc
    24541 ttatggtttg acagatgacc tcttgcgttt gtctattggg attgaggatg ctagagattt
    24601 gattgcagat ttgcgccaag ccttagaagg ataagacaaa gatgggaaaa tatgatttta
    24661 caagcctgcc caaccgttta gggcaccata cctataaatg gaaagaaaca gaaacggata
    24721 gtgaagttct accagcttgg atagcggata tggactttgt ggtcttgcct gaaatccgcc
    24781 aagccgtgca aacttacgca gaccaactgg tttatggtta tacctatgcc agtgaagact
    24841 taattaagga agttcaaaag tgggaagcta cacaatacgg ttacaacttt gacaaagagg
    24901 ctcttgtctt tatcgagggt gtggtaccag ccatctcaac agctattcaa acctttacaa
    24961 aagaaggcga ggcggtttta attaacacgc ctgtctaccc accctttgct cgcagtgtca
    25021 agttgaataa tcgtagattg attactaatt ccttagtgga aaaggatagt ctgtttgaga
    25081 ttgactttga ccaacttgaa aaggatttgg tggaagagga ggttaaactc tatgttcttt
    25141 gcaaccctca caatcctggt ggacgtgttt gggaaaaaga agtgttggag aagattggcc
    25201 aactctgcca aaaacacggt gttttgttag tttcggatga gattcaccaa gatttgaccc
    25261 tctttggtca caaacaccag tctttcaata ccatcaatcc tgccttcaaa aattttgcta
    25321 tcgtcttgag cagtgccact aaaacattta atattgctgg aacaaaaaat tcctatgcag
    25381 tcattgaaaa tcctaagttg agactggctt tccagaaacg cctgttggcc aataatcagc
    25441 atgaaatttc aggcttgggt tatttggcga cagaagctgc ctatcgatac ggtaaagatt
    25501 ggctagagga actcaagcaa gtctttgaag accacatcaa ttatgtggtg gatctatttg
    25561 gaaaagagac taaaatcaag gtcatgaaac cgcaaggtac ctacttgatt tggcttgact
    25621 tttcagccta tgacctgact gatgaaacat tgcaagagtt gttgagaaat gaagccaagg
    25681 ttatcctcaa ccgtggtttg gattttggag aggaaggaag tctccatgcc cgcatcaata
    25741 tagctatgcc caaatctctg ttgcaagaag tctgtcagcg gattgtggct acttttgcca
    25801 aatgttaaaa at
//
LOCUS       DAVMMR010000027        25563 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMMR010000027 DAVMMR010000000
VERSION     DAVMMR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.027"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(1..367)
                     /locus_tag="VYS74_001112"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="VYS74_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(429..878)
                     /locus_tag="VYS74_001113"
     CDS             complement(429..878)
                     /locus_tag="VYS74_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3789164.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(880..1638)
                     /locus_tag="VYS74_001114"
     CDS             complement(880..1638)
                     /locus_tag="VYS74_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3789165.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1753..3732)
                     /gene="pknB"
                     /locus_tag="VYS74_001115"
     CDS             complement(1753..3732)
                     /gene="pknB"
                     /locus_tag="VYS74_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV3789166.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKAQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3729..4469)
                     /locus_tag="VYS74_001116"
     CDS             complement(3729..4469)
                     /locus_tag="VYS74_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV3789167.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIVDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4484..5797)
                     /gene="rsmB"
                     /locus_tag="VYS74_001117"
     CDS             complement(4484..5797)
                     /gene="rsmB"
                     /locus_tag="VYS74_001117"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV3789168.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5790..6725)
                     /locus_tag="VYS74_001118"
     CDS             complement(5790..6725)
                     /locus_tag="VYS74_001118"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV3789169.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6738..9134)
                     /locus_tag="VYS74_001119"
     CDS             complement(6738..9134)
                     /locus_tag="VYS74_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV3789170.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9200..9514)
                     /locus_tag="VYS74_001120"
     CDS             complement(9200..9514)
                     /locus_tag="VYS74_001120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV3789171.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9539..10165)
                     /gene="gmk"
                     /locus_tag="VYS74_001121"
     CDS             complement(9539..10165)
                     /gene="gmk"
                     /locus_tag="VYS74_001121"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV3789172.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10295..11908)
                     /locus_tag="VYS74_001122"
     CDS             complement(10295..11908)
                     /locus_tag="VYS74_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV3789173.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(12252..14999)
                     /locus_tag="VYS74_001123"
     CDS             complement(12252..14999)
                     /locus_tag="VYS74_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV3789174.1"
                     /translation="MEENYNLSITQIKNSIKENSLVLFVGAGISANSNLPTWGELIQS
                     LKKELNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPNELHKLIEKIA
                     PKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGDFSKKNIVLKE
                     DDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNTFKLDAKNAYF
                     YTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLEVLSENRSQDV
                     NNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQLRFEIDGHEE
                     LRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKAKFRELANIAF
                     RQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILNSVTGDEKKII
                     EFFRDSILNFNFLYRKLETINELFDEIREEHESYRRGGWSANNHLFNAEFTVKNLYNF
                     LKLNCLCVEHYKIYKSIINRYLEILLLSYDNSYVNPDSSIFDRTSSWLKNLDLDDVQL
                     ILPNIDFKVVNLYFRNYSFGKIKVTEEAKDYLLNRITYLQERLEITEDENLRELKNML
                     IFLPLVDDIDIEKVIEILNNQTLYYNWREEFRRIIKIVLDNMDVIDKDSLKSKIIGIV
                     NKHLNEILEKNFSLYNSVYPLYSQLLEYCSTDQETAIIVLEKFNTDILRIKYKNDDIK
                     NIIEYSDLICHLFKYFEKDIKDDILDTLKVYEESENIIYHKVIDLMSYNVYDFPQIQN
                     KIYHYLIKRINDKRVEGVKTFPDPREKSVSDLYNLSRKGYFSDFEILKDIEEDIRGLY
                     PEVDWTWFHDRSDDVIHRLLEHRTPNNIKTYFSKNEEDNKLINEYILKAFDEDKLILK
                     K"
     gene            complement(15347..16444)
                     /locus_tag="VYS74_001124"
     CDS             complement(15347..16444)
                     /locus_tag="VYS74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV3789175.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(16454..17200)
                     /locus_tag="VYS74_001125"
     CDS             complement(16454..17200)
                     /locus_tag="VYS74_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3789176.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(17303..17656)
                     /gene="rsfS"
                     /locus_tag="VYS74_001126"
     CDS             complement(17303..17656)
                     /gene="rsfS"
                     /locus_tag="VYS74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV3789177.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(17669..18169)
                     /locus_tag="VYS74_001127"
     CDS             complement(17669..18169)
                     /locus_tag="VYS74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3789178.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(18170..18763)
                     /gene="yqeK"
                     /locus_tag="VYS74_001128"
     CDS             complement(18170..18763)
                     /gene="yqeK"
                     /locus_tag="VYS74_001128"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV3789179.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(18763..19392)
                     /locus_tag="VYS74_001129"
     CDS             complement(18763..19392)
                     /locus_tag="VYS74_001129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV3789180.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLVPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(19444..19755)
                     /gene="yhbY"
                     /locus_tag="VYS74_001130"
     CDS             complement(19444..19755)
                     /gene="yhbY"
                     /locus_tag="VYS74_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV3789181.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(19969..21075)
                     /gene="yqeH"
                     /locus_tag="VYS74_001131"
     CDS             complement(19969..21075)
                     /gene="yqeH"
                     /locus_tag="VYS74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV3789182.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(21078..21668)
                     /locus_tag="VYS74_001132"
     CDS             complement(21078..21668)
                     /locus_tag="VYS74_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV3789183.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(21620..22528)
                     /locus_tag="VYS74_001133"
     CDS             complement(21620..22528)
                     /locus_tag="VYS74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3789184.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(22585..23145)
                     /locus_tag="VYS74_001134"
     CDS             complement(22585..23145)
                     /locus_tag="VYS74_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV3789185.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            23284..24499
                     /gene="sstT"
                     /locus_tag="VYS74_001135"
                     /pseudo
     CDS             23284..24499
                     /gene="sstT"
                     /locus_tag="VYS74_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            24567..25520
                     /locus_tag="VYS74_001136"
     CDS             24567..25520
                     /locus_tag="VYS74_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3789186.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         7059 a         5809 c         4762 g         7933 t
ORIGIN      
        1 ccaagatttt accagcttgt ccgatatttc tgcgactcat tttgaacaac ttcatatcat
       61 gacaatagtt cacagcgata tccaaagaaa caattctccc ttgacttgtg acaatcgctt
      121 gaggttccat agaatgacat ttctatttta ccagaataat tggctagttg atttttttag
      181 gacgatttat ttttacctct gttgcatcca caatcaccgt attctcagca ctaagatgag
      241 tttttgaaat cgtaacacca ctttgaacaa gagttacttc aacccattgg ctccgacgga
      301 ttaagttgct ttcgtggata ccaaaatcag ccgtaatttc ttcataagtg cggtattctc
      361 gcacatatag aaagcgttgt taatttattt atctcatttt tcagaaaatt cttttatttc
      421 tgtaaagtct acgatactcg atgtgttttt atataatgat acagtctgag aatcactgtt
      481 ccgctagcca ttccaataga gattaccaga gccaacatga caaccaaggt cgcacttgcc
      541 agtgctttat tatagtcccc tgtcacaaaa aaggcagttg ttcggtagga gagataacct
      601 ggaaccagcg gtgccaaaat ggccaagata aagaccacag caggtgtctt ataaagaata
      661 cttaaaatct ggctgacaca agaaccaata atggctgcaa tgaaggtagc tacaatgaca
      721 ttggtcggtt ccttgagcaa gagatagatt agccagacag tcatgcccaa aatccctcca
      781 ggtaagagca tagaccgttg cacattgagt acgattaaaa aagtgataat ggcaagaaaa
      841 cttgctactg cttgtaataa aaaggttgtt agtgtcatat tagttcatca ataccaaggc
      901 gacagaagtt cctgccccta aagcgagggt aatgagcagg gattcaaaca tcttactcat
      961 accagagttt atgtggttgg tcataatatc acggaccgca ttggtcaagg caatgcctgg
     1021 tacaaacggc atgaccgcac cagctataat caaatctgcc gttgaaggaa aacctgtgta
     1081 gcgagcccaa aactgggcaa ttatcccaaa gacaaaggct ccagcaaagg ctgtcacaaa
     1141 gggaattcgg ataaattttt ccacatagag ggaaaaggca aaaccaaata aggtcgccac
     1201 tcctgcccca agtgcgtcgt agatatttcc gctaaacata actgaaaaga aaggagcact
     1261 aaaggtcgca gccagagtta cctgcaactt agtataggga aggggttgag cttgcaaggc
     1321 cgtcaattgc ttaaaggctg tttctaagtc aatctgcccc ccaactagct gacgagaaat
     1381 ctggttcaca tcgcagactt tttcgatgtt ataagaagag gaggtcacgc gcttcatgcg
     1441 cgaaatattg gtattttcaa tagagaaaaa gatagcggca ggcatggcaa ggacattgca
     1501 atccacaatc ccctgcgaat gcgcgattcg aatcatggta tcttctacac gatggatttc
     1561 tgagccactt ttaaggagaa tagtccccgc tagcataatc acatcaatga cggcatttaa
     1621 ttcttttgat tcttccatgc tttcctcctt ttatcaactc cctctattct atcacaaatc
     1681 tggactcaaa aaaaatcttt gccatgaaat catgacaaag attgattact cattttgatt
     1741 atccatctgc ttttaaggag tagctgaagt tgttttaggt ttgtagattg aaatcttgac
     1801 tctagtctta tttaggtcta ccttttcacc tgctctagga ctttgttcaa caaccatgcc
     1861 ttctacacta cctgcaggcg ctgtcgtcac ttctacaact tctatattag cttccttaat
     1921 cccaacaatt tgaatcaaat tgttcttagt aaactccaag ctggaaccaa tgtaactcgg
     1981 catggcaaca cttgtaactt ttttagctac tgtcaagaca atttgagtag gtttactcac
     2041 atcataagtc gttccggcac ctggactttg tttcataatc gttcctggtt cgctttcgct
     2101 ggactcttct tcctctatct taatcaaatt ctcaggaacc ttcttctgct tgagttctga
     2161 gattacttct gtagagttcc gtccaatata gttccctaat tgaatcgtcg tagctttttt
     2221 agctactgtc aaaacaattt gagttgcctt gctcaagtca taggtcgtac cttctggtag
     2281 actttgcttc aagaccgttc cagcctcact ctcattcgac tcttcttcct caattttaat
     2341 caaattatct ggaacttttt tctcttttaa ttccgcgata acatcagaag atttccggcc
     2401 gacataatta ctaatttgga aggattgttt gcctgatgag acaaccagat tgattttcgt
     2461 tccttctttt cgaccagttc cagcgccagg atctgtacgg ataatccgcc cttcttccac
     2521 cttttcacta gcctctgtct tctcctcacc aatctcaaaa ttggcttttt tgagcgttgc
     2581 cttggcctct gcaactgtct gacctgccac atctggaatg gcaatggttg caggagttct
     2641 ggatagtatc caaataagag aagctgccac caatacaagg ctggccaaca aaatcaggta
     2701 acgcatctta aatctatgtt ttttcggtgc ttgtggttgg taagtttcct ctgtcacagc
     2761 ctggcttggg tttttgattg atttgtgttc tgtctgcgct tgagccttag gaatagatgt
     2821 caaggtactc tgagaaacct tcggcaaggt cttggtatct gccttgctcg tttcatcaaa
     2881 gattaactta ctttcatttc tacgattgta ggacaagcta ctagacaagt ccacatacat
     2941 ctctgaaacc gagcggtagc gattggtcaa ctttttagca gtcgccttga taataacatt
     3001 ttctaaagcc tgaggtacag atggattttc tgcaataacg gacggcaggg gtttctggaa
     3061 atgctggagg gcaatggtca ccgcgctatc cccgtcataa gggatatggc ctgtcaacat
     3121 ctcatagaaa ataatcccca tggcatagat atcactctgc acagtcgcct tcgaaccacg
     3181 cgcctgctct ggtgacaagt aatgaactga gcccaacatc gagttagtct gggtcagact
     3241 tgtctctgca aaggctacag caatcccaaa gtctgtgacc ttggcagtcc catctggtgt
     3301 caaaaggata ttttgaggtt tcaagtccct gtgaacaatt cctcgagtat gggccaagcg
     3361 catagccaag agaatttgtc ccatgatacg gactgcttct tcattagaaa gaggataatg
     3421 ttccttgata tagcgtttga ggtctagtcc agcaacatac tccattgcaa gatactgttg
     3481 accgtcttcc tcaccaatat ctgttatccg aacgatatga ggatggtcta gatctgccat
     3541 agctctcgct tcacgctgaa aacgagctac agctatcggg tccgtctggt agttggtcct
     3601 cagaaccttc actgccactt cttccccatc taagattaag tctttggcta ggtagacatc
     3661 cgccatgcct cctcgaccaa tctgtttgac aatccgatag cgtccggcaa aaatcttgcc
     3721 gatttggatc attctgcatc ctcctcgttc atagaaacaa gggcaaccgt aatgttgtct
     3781 aaacctcctg cattgttagc aaaacgaaca agtgtctccg ttttatctgc taaaggaata
     3841 tcactggtta caatatcacg aatctcactg cctgaaatca tgttggtcaa gccgtcacta
     3901 ttgagcaaga gatagtcacc tgactcaagg ataactgtcc caaaatcagg ctgaatttca
     3961 tctttttgcc caatagactg ggtgataata tttttttgcg gatgagcttc tgcctcttct
     4021 ggtgtcaatt gaccagcctt gagcaattca ttaaccaagg aatgatcgct cgtcaactga
     4081 tggtattctt ctccacgaat caagccgata cgcgaatcac caatatgagc atagatagcc
     4141 tgattatcaa caatagcaag gacttccaaa gtagttccca tgcctctgta agcttcatcc
     4201 tgaccaagct ggtgaatctt ttgattttca atttctaggt aatgggcgaa ccattcacgc
     4261 acttcattga ctgtatcgat ctgggtatca acccaagcta cacccaggtc tgtgaccgcc
     4321 atttcactag cgatattccc tgcacgatga cctcccatcc catcagctaa aataatcatg
     4381 gtacgtccag ctctattgac atagtggttg acatagtctt ggttatttgt tcgtttctga
     4441 ccaacatctg ttaataatga aatttccatg tgtcagttcc ttcctaatcc gatatcttgc
     4501 gaaattgact gatgaagaat ccatcacttc catacaattc aggtgtaatg aggatacagc
     4561 cgtctttcat gatatcctta cattcatgtt ctagttttac ctgctcgaac tcgggatgac
     4621 tctctaaaaa ggccttaacg acttgaaaat tctcctctga gacgatagtg caggtgctat
     4681 aagttattat accacctttg cctagtattt gacaaacact acctaatatt tctaactgaa
     4741 tttcctgcaa ggacgcgaaa tctgccgttt ctttattgta tttgatatct ggttttcggc
     4801 gcaaaagacc gattcctgaa caaggagcat ccaccaaaat cttatcaaag gaatcctggt
     4861 caaaaaactc atgcaccttt ctggcatcca atttttgagt ttgaacccga tctgcaactc
     4921 ccagacgttg ggcattttct tgaattaaat ccaacttgtg gtcgtacaag tccagagcag
     4981 taacctgacc tgtcgtaaga taagaggcta tatgggctgt tttcccacct ggagccgcac
     5041 aggcatcaag cactcgctca tcaccttgta aatcaagcgt cggagcaacc aactgactgg
     5101 actcgtcttg gatggtaatg gctccatccg caaacaaatt atgccctgca aaatgcccct
     5161 gctccttaac cagaccagtg gttgctaaaa gggaattatt cgcctccaac aaggcttgga
     5221 tttcctcttt tcgacttagg tctgttacac gaatactggc tttgtttcgc actaacaggc
     5281 tttcaaagat ggcttttgct ctctcctctc cgtattcttc cttgagtttg gcaactagcc
     5341 aaactgggag agaataggca atggagtcac gcttgttttt tcgcttgatg ctagcaatat
     5401 ctggccagcc ttcacgcaag atacggcgaa ggacagcgtt gaccaatttt tcactgcctt
     5461 ttttacggag tttggccaat tccactgctt cattgaccac agcatgatct ggaatcttgt
     5521 ccaaatagcg gagttggtag gcactcatga gaagaaggac atagagccag ctgtctaact
     5581 ggtctctgtc ttcgataaag tgggataggt accattccag agtcagttta cgggctaccg
     5641 ttccatagac cagctcggtc actaagccct tgtctgctgc caaaagttga cttcccttta
     5701 gatgcttatt taaggcgata tttgagtatg cttggttcac aaaaacatcc tctagcactg
     5761 ctagagctaa acttctagcc gtttctactt tagtcaccaa atcgttctcc tacagtcaat
     5821 gtacgtccaa ctccgttgag gaaggaagca atgtccatct taggcttacc agctggctgc
     5881 acttgtttga gggatagagc cccttcagcc gttgcgacaa tcaattcttt cttgccgata
     5941 gagagaatct cacctggatt tccctgacct tctactggta gggcttcata aatcttaaag
     6001 cggtcgccct taaggaaagt atgggcaaca ggccaggggt tcattccacg aatttggtta
     6061 aagagttgac gattggtttt gttccagtcc agtttttctt cctctggctt tatatttgga
     6121 gagaaggtaa cctgactcgt atcctgcggt tcaggtttga tatcaccagc aatataggca
     6181 ggcagagtgt ccaaaagcaa atcacgacca actagcgcca atttttcaaa caaggtgcca
     6241 acattgtcct catctgtgat cggaatgctg cgacgagaaa tcatatctcc tgcatccatt
     6301 tccttaacca tttccatgat ggtcacacca gcttcctcat ccccttgaat caaggcataa
     6361 tggataggcg caccaccacg gtgtctagga aggagggagg catgaacgtt gacagcaaag
     6421 tccatgctat caaggagttt gcttgggaga aactgcccaa aagcagcagt cacaattcca
     6481 tctgctccta gcttcataag atcttccatc tctggacttc cagataattt ttcaggttgg
     6541 tagatagata gtcctgcttc cttggcagcc tgcttgactg gggtttcttg gataactttt
     6601 ttacgaccaa cagcacggtc tggctgggtc acaacggcta gaatttcgta acggtcatct
     6661 gtcaaaagtc cttttaagac tgttgctgaa aagtcggggg tccccataaa gattagtttt
     6721 gtcatatctt ctccttctta taaaaattgc tgcggctcat ggtcaatact gagacggagc
     6781 tcactatttt cccgttcttg agtcaaggcc aggacctggt tgagggtcgg ccccagctca
     6841 tcttctaaac ggtatttaat taaaatctgg taatgataga ggttgtgggt acgggcaatc
     6901 ggttttggcg ttggccccag aatgggactg gtctctgaca agcctgaccg caaaatgttc
     6961 atgacttcat aggcacgttt gaaaacctct tcttctttct tgtgagaaag ggtaatacca
     7021 atcgtgaaat agtaaggtgg atagccgagt tgtcgtctga ttcccatttc ataggcataa
     7081 aagccttcgt aatcttgatc cttggcaaat cgaatagcat agtgctgcgg attgtaggac
     7141 tgtatcaaga cttgacctgc tttttcagcc cgacctgctc gacctgccac ctgagtcaag
     7201 agctggaagg ttctctcaga agaacggaaa tcaggcagat tcaaggccgt atccgcattt
     7261 agaactccga ctagggtaac attgggaaaa tccaaaccct ttgcaatcat ctgagtacca
     7321 agtaaaatat ccgcttcccc tcgcccaaac tggtcaagca aggcttggtg actgcctttc
     7381 tttcgagtcg tatccacatc catcctcaaa atgcgagctt ggggaaagag ttctgctagc
     7441 tcatcataag ccttctgagt tcccgtccca tagtaacgaa tactgcggct cttacagtta
     7501 ggacagacct gaggaatatc cttcgagaaa ccacaataat ggcagttcat agtcttggta
     7561 tccatatgca aggtcagaga aatatcgcag ttgggacaag tatccaccgt cccacactcc
     7621 cgacacatga caaagctaga ataaccacgg cgattgagca tgagaaccac ctgctctttt
     7681 ttaaccagac ggtcttggat agcctctagc aaaggaggcg taaagtttga cgtctcattt
     7741 tgtccgatat agtctcgaaa gtcaatcact tgaacctcag ggattgtagc caaaggattg
     7801 gcacgttggg ttagacgtaa gtgttgatag acgcctttgc cagcacgtgc ccggctctct
     7861 aagctcggcg ttgcagatcc aagtaccaga gttgcttgat tatactgagc ccgtaaaata
     7921 gctacctctc tggcatggta acggggattg ctgtcctgct tataagccgc ttcatgctct
     7981 tcatcaataa tcatgacacc cagatttttc agaggagcaa agatagcaga tctggcacca
     8041 acaacaactt gggcatcgcc acgctccacc ttgcgccatt catcatactt ttcgccattg
     8101 gataatcctg agtgaagaat ggctaccttg tccccaaaac gtgctataaa acgctcggtc
     8161 atctgaggag tcaaggaaat ctcaggtacc agcaaaatag ctgtcttacc cttatccagg
     8221 gcaccttgga taatctgcaa gtaaacctcg gtcttcccac ttcctgtaat cccttgaagt
     8281 agaaagggag gttgagaact gccaatagaa ctcacaaccg catcacgcgc ctgtctttgt
     8341 tctggattta actccaaagg tctacttgct tcaattcctt caaaataagc agccgagcgt
     8401 tgaacttcct tttggactat ggtaacagca ccttgatcca caaagaagtt gacttgctct
     8461 cgcgagtagg actctaacaa gctagccaag gaagcgctct ctggatgaga cagcagataa
     8521 tctctcagtt ccaacttttt cttggcacgt gtagaaatct caacaccttc taattgggca
     8581 tggtcaacct cataccaaga ctgggtcttg accttctttt gatcgactgc ctgatattcc
     8641 agaccaagca ggccttttct agtcaaacgc atcatttcag cttgcttggc aaggtctagt
     8701 gaagaaaagg ctagcgaatc ttctgaacca aacaggcgta ctcgttcttc ctgactcaag
     8761 ccttccagag gatagagaat cttgtcatag ctagaattca gaaaccctgg aagcatggcc
     8821 ttgaggatag agattttgta ggagaagaca gatttgcgta actcctcagc cagccagagt
     8881 tgttctggcg tgagaacagg agaaaaatcc agcacctctg caatatcttt taaatcttgc
     8941 tccatctctt ctccatctga ttgggacttc aaaccaagaa caatcccttg aatcaggcga
     9001 ttacccttac caaaaggcac atgaacccgc atcccaactt ccagcattcc ctcaaattcc
     9061 tccggaatcc tgtaactata gggctggtcc gtctgcatca agggcacatc tacgataatc
     9121 ttagctaggg ccatcttctc acctcctcct tgtcagtaca ttcttgcaat agaaaaaata
     9181 agattgagtc cccccaacct taaatttttt caccatcttc tttttcttta gcaatttgct
     9241 ctttgatttt cttttcttct tcttctttgc ggcgtttttc ttcttcgata cggcgacgca
     9301 ctgcttcacg ttttccttct ggatctgggt gaattgtaac gtttcctgat tcgatttctt
     9361 ctaaagcgcg aagagttgat ttttcagact tgaaaccttg agttgctggg gcacctgctt
     9421 ccaattcgtg ggcacgtttt gcttccaaga ttacgagtga atattttgaa ggaaccttgt
     9481 cgagcaaggt atcaatagag ggttttaaca tcatttgctt gtacctattt tctaaatttt
     9541 atcgggtagt tggagatttt ggtaacatct cctgatagtg accaatgaca cgatccacac
     9601 agaagtgttc tgcttcaatc acacatttga cacgttcagc agctaggggc acctgatcgt
     9661 tgacaatcgc ataatcatac tcacgcatga gggcaatttc ttccttggcc ttttcgattc
     9721 gttgggcaat cacttctgca ctatctgttc cacgacctac caagcgatct tgcaattcat
     9781 ccaaatctgg tggtgtcagg aagataaaga cagcatctgg aacctttttc ttgacctgaa
     9841 gagcaccctg aacttcaatt tcaaggaaaa catcgattcc cttgtccaag gtttcattga
     9901 cataggtcag aggagttcca tagtagttgc cgacatattc tgcgtattcc aacatctgtc
     9961 cttgacgaat cagctcttca aattcttcac gagtacggaa gaaatagtca acaccgtcca
    10021 cttctccagg acgttgtgcg cgtgtcgtca tcgatacaga atattgaaat tggttttcag
    10081 aactctcaaa aatctctctt ctaaccgttc cttttccaac ccctgaagga ccagaaaaaa
    10141 cgattagtaa gcctcggtct gccattgtgt ctccttttag tcaatctgtg aaataacatt
    10201 tctctagaat aatggcaaaa agccagatta tcctttacag tctttctatc tagtgtaaca
    10261 aaaaagcagt aatttttcaa ctgctctttc ttatttattt agcataatct actgcacgaa
    10321 gctcgcgaat cacggttacc ttgatatttc ctggataatc gagattgttt tcaattttct
    10381 tacgaacttt gtgagccaag attgtgactt tgtcgtcctt gatttttcct ggattgacca
    10441 tgatacgaat ttcacgtcct gcttgaaggg caaagctagt ttgcactcct tcaaagccgt
    10501 tagcaatttc ttccaaatca tggagacgct tgatgtagct ttcaagagac tcactacgag
    10561 cacctggacg ggctgcgctc aaggcatctg ctgcagcgac gataactgct atcacgctct
    10621 cagcttcaac atctccgtgg tgactagcaa tcgtattcac cacaactggg ggttccttgt
    10681 acttacgggc caattccata ccgatttcaa cgtggctacc ttcaacctca tggtcaatgg
    10741 ctttcccgat atcgtgaagg aatccagcac gacgggcaag agccgcattt tcaccaagtt
    10801 cgctcgccat gataccagcc aacttagcaa cctcaatcga atggcgcaaa acattttgtc
    10861 catatgaagt acggaactgc aaacgtccca taatcttcat caagtctgga tgaaggtttg
    10921 gcgcaccaat ttcataggca gcagcctcac cgtattcacg aatcttattg tcaatctctt
    10981 gacggttttt ctcaaccaac tcttcgatac gagctggatg tatacgacca tctttgagca
    11041 acatttccat agtcatacgg gcaatctcac gacgaatcgg atcaaatcct gacaaggtca
    11101 ccacttctgg tgtatcgtcg ataatcacat cgacccctgt caaactttca aaggtacgaa
    11161 tgttacgacc ttcacgacca ataatgcgtc ccttcatagt atcgtctggc agatgaactg
    11221 ttgagtttgt tgactccgct acatattcac cagcgatacg ttgcatagct tgaaccaaga
    11281 tgtccttggc cattttgtca gaacgttcct tgacctcttg ctcagcttcg cgaatgcgac
    11341 tggcaatctc cctggtcaag ttttcctctg tctgagccaa gataatatct cgtgcttctg
    11401 cctgagacag agcaccaata cgctctagtt ctgcttcttt ttgtctttcg acttcctcta
    11461 attgctcttc acgcgcatca aggtttttcg ctctatcaga aatactttgt tctttttgtt
    11521 caagtgtttg ttctttactc gtcaaattgt cgtccttacg gtcaaggcta gtagctctct
    11581 ctgtcaaacg actttcgatt tgtttgagtt cttgacgttc tgatttgaat tcagcgtcca
    11641 cttcttcacg gtattttctg gcttcttctt tggcctccaa tagtgcttct tttttaagag
    11701 acttgctttc acgtttagct tcattaacaa gtaaatccgc ttcacgctca gcttgtccac
    11761 gtaaattagt tgcttcttgt tcagcattta aaagcatcaa ctccgcagct tcctgagatg
    11821 atttcatctt agctgagatg ctgacatatc caatgactaa accaatgatg acggcaaaaa
    11881 cagcaatcgc aagcgacatg atttccatgt ttttacctca ttttattgtt attccgaatg
    11941 acatacattc ttttacattc taccataaaa aagtgatttt cacaaaccta aaatagaata
    12001 tgttttgggg aatttagtct gaattttagg attacacttc cttggttttt taaggagata
    12061 agtcagtgtc gctatcaaaa agttccaccg aatttctaca actatggatt ttctattgtt
    12121 gtgccaaata gtgttccact agattcaaac attttgcttt caagtctttg aaaattcact
    12181 ttaggaaatt acagcttatc ttcaagatca aatttgtcat aaattaactt tttttattta
    12241 gttgtatcaa tttatttctt aagtattaat ttatcctcat cgaaagcttt taaaatatac
    12301 tcattaatca atttattatc ttcttcattt tttgaaaaat aagttttaat attattggga
    12361 gttctatgtt ctagtagacg atggataaca tcatcacttc tatcatggaa ccaggtccaa
    12421 tctacttcag gataaagtcc tcgaatatct tcttcaatat cttttaaaat ctcaaaatca
    12481 gaaaaatatc cttttctact taagttgtat aaatctgata cagacttttc tcttgggtct
    12541 gggaaagttt taaccccctc aactctttta tcattaattc ttttaatcaa ataatggtag
    12601 attttatttt gtatctgagg aaaatcatag acattataac tcatcaaatc aattacttta
    12661 tggtaaataa tattttctga ttcttcataa acttttagag tatctagaat gtcatcttta
    12721 atatcctttt cgaaatactt aaataagtgg catatcaagt cagaatattc tatgatattt
    12781 tttatatcat catttttata ttttattctt aaaatatctg tattaaattt ttcgagtact
    12841 attattgcag tttcctgatc agttgagcag tattctagta actgtgaata gagtggatac
    12901 actgaattat ataaagaaaa atttttctcc aaaatttcat ttaaatgttt atttacaatc
    12961 ccaattatct tagatttcaa tgaatcttta tcaataacat ccatattatc gaggacaatt
    13021 ttaataattc tcctaaattc ctctctccaa ttataataca gtgtttgatt atttaaaatt
    13081 tctattactt tttctatgtc aatatcatca acaagaggaa gaaaaattag catattttta
    13141 agctctcgaa gattttcatc ttcagtaatt tcaagtcttt cttgaagata agtaattcga
    13201 ttcaacaagt agtctttcgc ttcctcagta acttttattt taccaaatga atagtttcta
    13261 aaatataaat ttacaacctt aaaatctata ttcggaagaa ttaattgaac atcatccaaa
    13321 tcaagatttt taagccaaga agatgttcta tcgaaaatac ttgagtcagg atttacatat
    13381 gaattatcat aactgagtaa taatatttct aaatatctat taatgattga tttataaatc
    13441 ttataatgtt caacacataa acaatttaat tttaaaaagt tataaagatt ttttactgta
    13501 aactccgcat taaatagatg attattagca gaccatcctc ctcttctata gctttcatgc
    13561 tcttcacgaa tttcatcaaa taattcatta atagtttcta attttctata taaaaaattg
    13621 aagttaagga ttgaatctct aaaaaattct ataatctttt tctcatctcc tgtaaccgag
    13681 ttaagaattt gttctgttaa ttcacttaat tctccatcat aaagtggctc tgcataacta
    13741 ggtgtttttt cgtatctatc aattatttga atttgctgaa aattaaattc acaaactaag
    13801 aaattaaaat agtctttctg acgaaatgct atattagcta gttctctaaa ttttgctttt
    13861 gctaaagaat attgtttttc ttcatacaac ttaaaagcct tacttaagaa tcgattcccc
    13921 tttaaatccc taggctcacc aatctccata tctagaaagc gatttaataa agatttttta
    13981 gataccataa ttcgtaattc ttcatgacca tcaatttcaa atcttaattg atcatttcca
    14041 aaccagcaat actcatcatc ccagtttaaa gcccttttct ttaaatcaga atatctagaa
    14101 aaatctttcg catcaataaa actaagtcta tctaaaaaag ccagttgatt ccataaatca
    14161 tcagcattat ttacatcttg acttcggttt tctgatagaa cctctaaaaa atcttttgtc
    14221 ctgcaataca atttgttctg tttttctgat gtctcctgac ctagattctc ttcattagag
    14281 ataatgaaaa ttccttgctt cttataatac tctcgaatga tcatagacgg ttcctcggga
    14341 gtataaaaat aagcattttt agcatccaat ttaaatgtat tttgaatcat tctaaaaatt
    14401 gaattaaaag tcgaatcact tagagaatac cccacaaaca atagagtatg attcattatc
    14461 aatgactgga ttaaagtaga aatcattggg aagttcaaat gatagtctaa ataatcatct
    14521 tctttcaaaa caatattttt cttactgaag tcaccatgca ttttaatcaa gtatctttcc
    14581 gaactcgtat aagggatgtc tttatcctca gcgactacat tgtactttaa caagccactt
    14641 tcaaattgac tctccagaag tgaatcatag tttgtcgtaa taatatgttt tggtgcaatt
    14701 ttttctatta atttatgtaa ctcatttggt ttactcaatc ccttcttaaa gaaaatttct
    14761 tctatcttct ttgtgtactg atttttccca aaagtatcat aataatactg agcaattctc
    14821 agtggactat ctgttctttc ttctggtatg ttcaattctt tttttaatga ttgaatcaat
    14881 tctccccaag ttggtagatt agaattagct gatattcctg caccaacaaa caaaactaag
    14941 gaattttctt taatagaatt ctttatttga gttatagata aattataatt ttcttccata
    15001 ttttaactat cctatcatta gcttgtatta cataaataca aattattctt ataaatcttc
    15061 ttttttatta cataatctta aacaaaagtt tatcatggtt tataaatata aaaatttatt
    15121 tgtcttttat attcaattta tgatttttaa tataaccagt tctcttcatt ataccacaaa
    15181 ataacaagca taaagcggat tcaaagataa agattaaggt tcatatacta attttcttta
    15241 ttgacttttt gtcttctgta ttagggagtt tatcattttc caaaaacttt atcttcaatt
    15301 tgttagctaa aatttctaca ataaaaaagc ctacctttca gtagacttag tttgtttcta
    15361 ttctaatcgg cactctgcca aaattctgtt ctgctatact tggatttccc aattggtaaa
    15421 tctggtctgc cttttgagtc atgacatccc aaggttcctt gccaattctg ctgactagac
    15481 tgacctgccc tttcagatat ttaagatgtt gcctgccttt ttcggtaaag ccaaggacat
    15541 gaatcccttc tggcaagtca ctttctctag cctgcaccaa gatataggtc aagaggcgtc
    15601 tgacacgcgc cttggtgtaa cgtttggtgg tcactaactc gaccaattct tccacagact
    15661 gggctgtttt aatagcttct ttaatgcgca cagccatttc ttgattgacc tgatagatgg
    15721 tggttaggtc gggatttgac aagatttgat agcggagcaa gggaaaatag tcttcccaga
    15781 tcaccttact ggcctgctca aatagggcaa cagaaggcat aaaacgttct aagaaatctt
    15841 ggtccttctg atgttgacgg agggctgtcg ccgaggcaaa gtccacatct ttattcacag
    15901 aatggtaacc tgccccctga cgctgaatcg gatgcagctt gatatttcgt cctgcaaccg
    15961 cctttgcata ggccagagca agaacatgat tgggggtatt acctgaaaaa tcaagaccag
    16021 caaattcctt ccacatagct tgggttttct ggggatagga aagggaatca ggcagatttt
    16081 ccacaaattt ctccatctca gcacctttct ctgtgtataa gtcagcgatt ttctgataat
    16141 ctcgaacttc ctctgtccca aaaactagac tatcaatgcc caagcgatcc aagatatcca
    16201 cagctccttg accaaagaaa tcagctgcct gaacactgac taaaaagggc aattccacta
    16261 ccaagtctgc gccattttcc agcgccatct gggcccttgt ccacttatcc acaatagcag
    16321 gctctccacg ctgcatgaaa ttccctgaca tggcaacaat tttcagtccc tctgcctgat
    16381 ccagcaggta tttatggcca ttatgaaaag gattgaactc cgcgataata cctgtgatgg
    16441 tcatgctaat ctcctacttc tgcgccacaa aaaaccaacg ggtgctagtt tctgttggct
    16501 ccttatcctc aaagtctgca tagagtttga aggacttgaa tccagcctgt tccagcaaga
    16561 tatcataggt caagatctca taggtcctct cctcatgtac ttcatcgtgg cgactaaagg
    16621 aaccgtcagc ctccttgata aagaaagtca gctcatgcac gatggagtga ggaacttcgt
    16681 cctcatatgt atcccaaagc atggcaaaat cttccgcatt ttcatggtag gaatagccag
    16741 ggaatacttc atctgtctgg taggtcgagt gcacgtcaaa gatgaaaact ccctcttcat
    16801 tcagcgcatt gtacacgtcc ttaaagacgt cccctacttc cacctcatcc tgcatatagc
    16861 agatagagtc cgaataacac gtgacaaaat cgtatttccc tgccttggac aaatttagca
    16921 tattaccttc tataaaagca atcttttgct tggctgaagt tgctctcttc tccgcaatct
    16981 tcaacatatc cgcactcaag tcaagtccag tcacatcaaa accagcctga gagaagcgca
    17041 ctgattgaat tcctgtccca caagccaatt ccaagagttt ctttctctcc ttggtcttag
    17101 gcaaatgacg cagagaaaaa ttcgtccatt tgtcgtataa actatcgtcc atgaccgcat
    17161 catagacagc cgcaaaggtt tcataagtcg ccataatcat gatactaaga gctccatggt
    17221 aaacaccact agccctttac ctttctatca atttttctaa aataagcaaa gaaacccagt
    17281 tgactacaac tgagttcatc ttctaagcaa gagcttctga aatatctact gaattcgcct
    17341 catgccatag cttctctaga ttataatggg cacgcatttc ttctgaaaag atatgcacga
    17401 caacagcacc gaggtccagt aagacccagc ctccagctgc atcgccttcg acatggctac
    17461 ctttaaagcc tgcttgagct actttttcac ggatattagc tgcgatagcg tccagctgac
    17521 ggctattcat tgagctagtg atgacaaagt agtccgtcac actagtcaaa tcttgtacat
    17581 caagtgcgag gatatcctcc gcacgtttct catcagccgc tttcacgact agttctagta
    17641 attctttttc gttcatttaa tcctcttttt agaaaatatg tttgtaatct aggacatctg
    17701 caaagcgctc ctcccaaatg tcctcataaa attcattaat cgtttctgct ggaatgtcat
    17761 ccccatcaaa ggttgtgaca gcaccttctg gaacaataag ctgatagcca tattcaaagg
    17821 caatcttgac agaggtatcc acacaatatt ctgtctgcat accacataaa actaattttt
    17881 caatcccctg cttatccaag tattctttta agccagtttc tttgaaaata ctgttatatt
    17941 tcttctgaaa gaccttttca gcaggttttc gatttaaaag cgcagataac tgccaatctt
    18001 ctgatgtttg agcttcagag ttctcaatat gttgaacata gataatttca atattcttgc
    18061 ttctagcctg gttttgtaaa taagaaattt tttccaatag actttttgtc tggaacccag
    18121 tttctactaa aacattctga acatcaatga ttataaaagc catcttcatt tagttctctt
    18181 tcaaatagtg cacaaaggcg ttataggttt caagggtttg gggatagatg gggaatccct
    18241 gatgagctag atactccacg gtacgagctg tttcgtaggc caccgcctta ttgagcgata
    18301 gacttgcaac ctcacgcgcc acatctactc caggaaagga tcgattgtgc tcgatatagt
    18361 ctgctacata gacaacctta tctaggtctg tcatctgacc agcaccgact gtatggactt
    18421 caatggctcg caggatttct gaatcatgca aatccaaatc ttcctgaatt ttgtaaattc
    18481 cgaccatacc atgccagaca ttattgcccc agtttttgag gccagggtct agctggtaac
    18541 ggtcaatcaa gtctagaaat tcctgatctg acaacttttt agcatagtca tgaagaagac
    18601 ctgctagact tgctttctca acatctacac caaagcgctg agctaattcc atagcggcac
    18661 gctccacacc tagacaatgg gttagacgtt tctcaggtag aagctctgcc attttttcca
    18721 acaaggcctc acgggagcag ttgatatagt cttgatagga catcagtaga gcccctcctt
    18781 ctcgatgtag tctagcactg gctgaggtag gagaaagttg ggtttccgac cttgggcaat
    18841 gaagtcccgc accatgctgg acgagatatc catgagcggt acgtccaccc agataactgg
    18901 ataggaagtc cctgccttgt agcgtggacg ctgaaccccc acaaactgaa ccatgtcaac
    18961 cagttcatca attcggtacc acttaggcag atagtcaacc atgtcggcgc cgatgataaa
    19021 gtaataatcc gtatctggat gttgctctgt caagattttc atggtgtcgt aggtgtagga
    19081 aatacccttg cgctccaact caatggtttc aatgactagg ccgtcaatcc cctcaattgc
    19141 caactcaagc atcttgagac gatggtgttc agggatggtt tcctttttat caacgtgagg
    19201 aggttggtat tcaggcacga gaagaacttg atccagtccc aactgttgcc gtacttgatc
    19261 cgcaacaatg agatgggcat tgtgaacagg gttaaaattc ccccctaaaa tcccaacttg
    19321 tttgcgtttt ttctccttga tttctggctc caactctacc ttggtaaagg gagtcaatag
    19381 ttcgattgtc ataggctagt cccctttatt tctctataaa aacagttatt tggagtttcg
    19441 gtcttagatt tctttgactt tcttagaaat cttgcgattt tctttcttgc tagattgttt
    19501 aaacaaaatc aagatgcgtc ctattttttg gactgtatcc acaccgattt cttcttccaa
    19561 aatttcagct acttcgtgga tgttttcatc tgtgttttgt aagagagtaa ccttgattaa
    19621 ttcacgcgca tcaagagctt gacggacgct ggttttgatt tggtcgttga gtccattttt
    19681 cccgatttgg atgataggtt tgagggtgtg tgcctggctg ttgaggaagg cacgttgttt
    19741 tgatgttaat gacataattt tcttccttgt tttttgatag ttattttagg tggtgaggga
    19801 cggtcggaac taattttcca aagatttcat ctttgaaaaa tggatttcct gacctcacaa
    19861 ttgagccttg gtctcaattg tgccccttgc caatctacag attggcaatc acaccacggc
    19921 aataactatc gctatatgag ctcactttgt tcgctcagcg attttgtttt aaataattgc
    19981 ttttcgtgta acgacggcga cgccttctgg tgcccagacg gcgacttttg ctgtgcctgt
    20041 tacacgaatc cagcctaggc ctgagatgac taggtctgtc ttatctttaa tggtaaagac
    20101 atgctggact agccttggga aatcttcttt ttccttgcta tttggtggtg tcagaagagt
    20161 tcccaggtgc ttatcgtaga aagcactagc tccttcaagc ttgctacgat ggagtttgag
    20221 ttcattatca aagaaagcag taaatccttg cttttctcct gctataaagt caaagcgtcc
    20281 caaaccacct aaaaataggg tttgctcagg attaagctga taggtcttag gcttgatttc
    20341 ctttttagga ctgacatact tgaggttttt ggccgtcaag tagtgagcca tctggtggcg
    20401 gtggataatt cccggcgtat cgtaaatata agatccgtcg tcaagcggaa tctctatttt
    20461 gtccaaggtt gtccctggga agcgtgaagt agtgatgaca ttctgatcac ccgtgatttc
    20521 ttggataata gcattgatta gagttgattt tccaacgttg gtcacaccga ccacatagac
    20581 atcgcggccc ttacggtagt gttcaatctt gtcaatgact tccttaatgg catgtttatt
    20641 ttgtgctgaa gttaggacca catcgactgg acgaagacct tcttcatagg cacgtttcat
    20701 gagccactgg ctaatcttac cagacttaac tgacttagga aggatatctt ttttatttcc
    20761 taccaagagg acatcattgc ccgagacgaa acgtggtaaa cctgggatga cagatccatt
    20821 aaaatcaaag atatcaatga cattgaccac taaagcatca ctgtctccca cctcgtgcaa
    20881 gagcttgagg aaatcatcgt tcgtcaactg gacatctgtg atttcattgt agtggcggag
    20941 acggaaacag cgttggcaat agacttcgcc agtctccaaa cctttttcaa gtgccgactg
    21001 gggggtaaaa ccaagaccag ctttatctgt cgtctgaatg gttgctccac aaccaataca
    21061 gagaatttct tccatagtta aattcctttt ttatatgtaa tcggtccgta cttttcagtg
    21121 atttttctca taacacgacg ctcacgagtt cggttaatct gcgttttgat tgagtcatgt
    21181 tggaccaagg gtttgactaa aattgaccga atccctgcac ggtgggctgc tcgtatatct
    21241 gtcatgagct ggtcgccaac catgactact tcctttttgt catagtggaa ttccttcatg
    21301 gcacggtcaa taccaaatgt gaagggcttc aaggcccagt aaacgtaatc aatcccaaat
    21361 ttctcaactg ctcgttgaac gcgttttttg gtgttatttg acactacgat aatgccaata
    21421 cccgcgtccc gaaggtcatg tagccattgc ttcatctctg gcgttccatc agggttgttc
    21481 caagcaatga gggtattatc caaatcgacc aaaacagcct tgattccctg cgcctgcagg
    21541 cttgggactg tcagatcata gactgcttcc acagcaaaat ctggtatata attttcaatc
    21601 gccattctgg ctccttttct taactttttt tcgcaatttt ccttagtaac aaggataaaa
    21661 caatccacaa acctgcacta gccaaactga tatagagcca agcatggggc tcatctgtta
    21721 aaggtaaggg aacattcatt ccgaaaaagc ctgtcacgac tgccaaaaca gctagcaaga
    21781 ctgaaatgat agtcaaggtt gtcaaattgt cattcagatt attgtttaga atattgttgt
    21841 aagaggctga aagctgctgt aaaatctgag agattaggtc tgtcatggat accagctgat
    21901 gagcctcaat catggcatca tcaaactgtt ctctctcaat ctcatcaaaa ctacgataca
    21961 aggcatgacc ttgaatatgc tctaacaaaa tccgattttg tttggcagct gccgtcagat
    22021 aaaccatacc agtctccaaa tcagacagga caaagaggtt tttcttggta gttcgctggc
    22081 gcaagaggtc attgacctca tccctactct tgtccatctg ctcaatgaca ggatagtagg
    22141 cattgctgat aatttccaga ctggcaaaga gaaacttata aatcgaaagc gtgtcatggc
    22201 tctccagata acgagtcatc tgttcaatga cataggcgtt cttggtatta ctaatggtaa
    22261 tcaggcgacg atgctcgaca ataaaggtca tgggaaaagt ctcatagtag gccttgtcct
    22321 tttttacatc taggacatta tagataaagg taaccgtccc actttcacgg tggtagtcca
    22381 tgtgggcgcg ctcgtttcta tccagtgcgt attcaatggt ttccttgtcc aaaccgtaaa
    22441 tttcagaaag gtcttctagt ttattcaact ttcctaggtc taggtctatc caggtacaac
    22501 cattgcccaa ctgcttttct aaaaccatct tttctcctta ttaaactccc tctattgtac
    22561 cacaaattac ttaaaataac aggtctagtc tgtgcgtgag aaattgctaa caactgtgat
    22621 atttcggtta gggacaaagt gaagggcttg ctcctcgcta cgaagctgtg tcgtacgaat
    22681 tcccacactg acaaccttac ccgatacagt aatcggtcca tttgtcagaa cgacctcatc
    22741 tcccacatcc agttgacgtt caaagaggat gaaaaagcca ttgatgacat cagacagaaa
    22801 gccttgggct cccataccaa tcgctacccc agcaatacca gctccagcca gcaaactaga
    22861 aactggcaaa cctaaaatcg acaaaatgca gtagagtaaa aagaaatata gcgtataatt
    22921 aaacacattt tctagtaaac gtgagatggt tttttggcgt ccgacatcat gacgagacat
    22981 ttttagagaa ggtttgacaa ttctctgcac catggtatga agcatttttt tagcaatata
    23041 aaatacaatc aaaagcagta aaagagaaat gaccttggtt agaatattct cgataatggt
    23101 tgtcacatct agcttttcaa tataagcctg aataaatttt tgcatataaa actcctttat
    23161 ttcattatac catattttca tcagtctcat tctccataaa tattcaaaaa atagacagaa
    23221 aattcttgat tttgtctatc atttatacta taatcataat cactacacaa aaaggagatt
    23281 attatgaaaa gaatcattca tgcttggaat aaggcaagcc ttgtcaagcg tatcttgatt
    23341 ggtatgcttt gtggggaatc ctaggactga cccttcctaa tctctcaggg attgggctac
    23401 tcgaggattt attcgttgga ggtctaaaag ctgttgctcc tatcctagtc tttgctctcg
    23461 ttgccaatgc cctttcccaa catcaaaagg gacaagatag caatatgaaa actgttgttt
    23521 tcttgtatat cctatgatag ggactttcgc cgctgctctt gtagctgtac tagcaagttt
    23581 catcgtccct attgaaatta ccctaaatag tgccaatact gaaattgcac caccagatgg
    23641 tattgggcag gtcctcagca acctcttgct caaactggtt gacaacccag tcaacgccct
    23701 gcttactgct aactatattg gaatcttatc ttgggcagtc atttttggaa tcgctatgag
    23761 agaagccagt aaaaatagta aagaattgct aaaaactatc gctgacgtga cttctaaaat
    23821 tgtcgaatgg atcatcaatc tggctccatt tggaatcctt ggtcttgttt ttaaaaccat
    23881 ttctgacaag ggagtcggaa gccttgccaa ctacggtatt ttattggttc cattagtaac
    23941 gactatgctt tttgttgccc ctgtggtcaa ccctttgatt gccttcttct ttatgagtat
    24001 gagacgcaat ccttaccctc tagtttggaa ctgcctccgt gtcagcggtg tgacagcctt
    24061 tttcactcgt agttctgcga ctaacattcc tgtcaacatg aaactctgcc atgaccttgg
    24121 actcaaccca gatacctatt ctgtttctat cccactcggt tctactatca atatggctgg
    24181 agtagcgatt accattaacg ttttgaccct tgttacagtt aacactcttg gaattcctgt
    24241 tgactttgcc acagcctttg tcctcagtgt ggtagcagct atctcagcct gtggtgcttc
    24301 aggtattgcc ggaggttccc tccttcttat cccagttgct tgtagccttt tcggtatttc
    24361 taacgatatt gccatacaag ttgttggggt tggttttgtg attggtgtca tccaagactc
    24421 atgtgaaaca gcccttaact cttctacaga tgtcctcttt accgccgttg ccgaatacgc
    24481 agcaacccgt aaaaaataac tcatcaaggc aagcctgctc atgtcttgtc ttttacgctt
    24541 ttattctaac ttattaggaa attcttatgt ctattagcca acgtacgacc aagctcatct
    24601 tagctacctg tcttgcctgc ctgcttgctt attttctcaa tctttcgtca gcagtttcgg
    24661 ctggaattat cgctctcttg agcctatctg atacgcgtag aagtacttta aaactggctc
    24721 gcaatcgtct tttttctatg cttctagctc tggctatcgg tgttctagct tttcacttga
    24781 gcggatttca tatctggagt ctcggcctct atctggcctt ctacgttcct ttagcctaca
    24841 agatgggctg ggaaattggc atcacaccaa gcacggtttt ggttagccat ctcttggttc
    24901 aagagtcaac ctctccagac ctcctagtca acgaattcct tctctttgct attggtacag
    24961 gatttgcctt gcttgttaat ctctatatgc cttcacgaga agaggaaatc cagcactacc
    25021 acacgctggt ggaagaaaag ttaaaagata tcctccagcg cttcaaatac tatttatcca
    25081 gaggagacgg acgcaaccga gcacagctgg tagcagaatt agacacgctt ttgaaagaag
    25141 ccctcagact ggtctatttg gatcactctg accacctctt tcaccagacc gactaccata
    25201 tccactactt tgagatgaga caacgacaaa gtcgtatcct gagaaacatg gcccaacaga
    25261 ttaacacttg tcaccttgcc gccagtgaaa gcctgatctt agcgcaactc ttttcaaaaa
    25321 ttgcaggtca actgagccag accaatcctg cttctgattt gctagatgaa attgaacgtt
    25381 atctggaagt cttccggaac cgcagtctgc ccaagacaag agaagaattt gaaacccgcg
    25441 ccacccttct tcaactccta cgtgaagcca aaaccttcat ccaagtaaaa gttgattttt
    25501 accaaaaata tagacagtaa tactcttcaa actacgtcag cttcaccttg ccgtatatat
    25561 gtt
//
LOCUS       DAVMMR010000028        25249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMMR010000028 DAVMMR010000000
VERSION     DAVMMR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.028"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(1..326)
                     /locus_tag="VYS74_001137"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="VYS74_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            874..3432
                     /locus_tag="VYS74_001138"
     CDS             874..3432
                     /locus_tag="VYS74_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3789187.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            3732..4571
                     /locus_tag="VYS74_001139"
     CDS             3732..4571
                     /locus_tag="VYS74_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV3789188.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            4589..6427
                     /locus_tag="VYS74_001140"
     CDS             4589..6427
                     /locus_tag="VYS74_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3789189.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            6440..7855
                     /locus_tag="VYS74_001141"
     CDS             6440..7855
                     /locus_tag="VYS74_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3789190.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            8447..9493
                     /gene="pheS"
                     /locus_tag="VYS74_001142"
     CDS             8447..9493
                     /gene="pheS"
                     /locus_tag="VYS74_001142"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV3789191.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            9493..10002
                     /locus_tag="VYS74_001143"
     CDS             9493..10002
                     /locus_tag="VYS74_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789192.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            10079..12481
                     /locus_tag="VYS74_001144"
     CDS             10079..12481
                     /locus_tag="VYS74_001144"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV3789193.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(12549..13550)
                     /locus_tag="VYS74_001145"
     CDS             complement(12549..13550)
                     /locus_tag="VYS74_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV3789194.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            13706..14152
                     /locus_tag="VYS74_001146"
                     /pseudo
     CDS             13706..14152
                     /locus_tag="VYS74_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            14278..14544
                     /locus_tag="VYS74_001147"
     CDS             14278..14544
                     /locus_tag="VYS74_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789195.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            14805..17054
                     /gene="metE"
                     /locus_tag="VYS74_001148"
     CDS             14805..17054
                     /gene="metE"
                     /locus_tag="VYS74_001148"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV3789196.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            17118..17984
                     /gene="metF"
                     /locus_tag="VYS74_001149"
     CDS             17118..17984
                     /gene="metF"
                     /locus_tag="VYS74_001149"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV3789197.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            18669..20882
                     /gene="pnp"
                     /locus_tag="VYS74_001150"
     CDS             18669..20882
                     /gene="pnp"
                     /locus_tag="VYS74_001150"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV3789198.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            20898..21515
                     /gene="cysE"
                     /locus_tag="VYS74_001151"
     CDS             20898..21515
                     /gene="cysE"
                     /locus_tag="VYS74_001151"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV3789199.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            21527..22411
                     /locus_tag="VYS74_001152"
     CDS             21527..22411
                     /locus_tag="VYS74_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789200.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            22498..23841
                     /locus_tag="VYS74_001153"
     CDS             22498..23841
                     /locus_tag="VYS74_001153"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV3789201.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            23834..24220
                     /locus_tag="VYS74_001154"
     CDS             23834..24220
                     /locus_tag="VYS74_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV3789202.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            24224..25108
                     /locus_tag="VYS74_001155"
     CDS             24224..25108
                     /locus_tag="VYS74_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV3789203.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         7722 a         4466 c         5581 g         7480 t
ORIGIN      
        1 aggcagattt ctaaaattac actcaaagtg gaagtcattg agctttcgaa tgacagttga
       61 agttgaaatg gccagctgat gggcaatatc ggtcatagaa atcttttcaa ttaacttttg
      121 cgcaatcttt tggttgataa tacgaggaat ttggtgattt ttcttgacga tagaagtttc
      181 agcgaccatc attttcaagc aatgatagca cttaaaacga cgttttctaa ggagaattct
      241 agtaggcata ccagtcgttt caaggtaagg aattttatag ggtctttaat gtctagtaat
      301 tttgtgataa aatgtaattg ttccatatga ttctttctaa tgagttgttt tgtcgctttt
      361 cattatagat cttatgggac tttttttcta cccaaaatag gctccataat atccataggg
      421 aatttaccca ctacaaatat tatagagccc aaagttttag gtcgcttgat aatatgcgtt
      481 ttttgaattt tatagactgc tcgtttaaac tctatttact tcgtaccttc tggagcgaga
      541 cggaatatta gtcacataca aaatgagtac tattaggatt ttattttcat gtacaatttc
      601 agccagtctt gttataatca gcctatagga atcaaggagg tgactcttat ggctgttttt
      661 gtgtctttgg atggaattgt ggtagaagtc cttgatgtct tttcttcttt taatggggat
      721 agtgagtttt tcttgtgtat agcattttga atctggaata ggacgccatg actgctaaaa
      781 gatttctata aattaatttg attttcctaa tcaatttgtt catatcttat ttcattccac
      841 tataaacgtc ttaaagacaa gagtcagttt gttatggaac gctctcagtt cgaggagatg
      901 ttccaacttc aaagtagtcg cttgacgacg caagaaaaat tacaattgtt tacctctgtg
      961 tttgctggcc gttatgatgt ttatgctaag aattttatca atgaacaagg gaaaattcag
     1021 tattttcctt cctatgatta tggttggaag cagttgccac ctgaaaaacg gagtttccag
     1081 acattgacga actccgtttt gaaatctcat tttcgtgggg aggcagctat cggtatcttt
     1141 cctatgcact tagatgatag ctgttatttt ttggtactgg atttggatga aggagattgg
     1201 aaagaagctg gtttaaccat tcgaagaata gccagggaac gccagatgga agcccattta
     1261 gagatttctc gttcgggtta cggactccat atttggttct tctttgagga agcgattctg
     1321 agtcgaaagg ctcgcctgtt tggaaagaaa ctattagaat tggctatgca ggaaagtatg
     1381 caactgtcct ttgattcttt tgatcgcatg tttccaaatc aggatgttct tcctaaaggt
     1441 ggatttgata atttaatttc ccttcctttt caaggagaag cttatcatca agggcgaacg
     1501 gtctttgtgg atgaacagtt tcagccttat gaagatcaat ggaggtatct acaagaaatt
     1561 cagaagattt caactgctaa agtggcactg ttaatccaag aggagttagg caagcaagaa
     1621 ttggacaagg agttgaagat cgtcttatcc aatatgatcc aacttgaaaa atcgtctgtg
     1681 acacccaaga cactgttttt cttgaaaaat atggcttcat tttctaatcc cgaattttat
     1741 ttaaagcagg ctatgcgaca gccaacctat caaattcctg agagaatgta tttatttgga
     1801 gaatccgatc attatttatg gttgccaaga ggtttgctgt atccattgca agataaattt
     1861 aagcaggtat ctgtggaaga taggagaaag gtacaaaggt ctattagcgt ggaatttaag
     1921 ggagaactca cttttgagca agagttagcc ctgtcagata tgacttctaa agaaaatggt
     1981 ttacttcatg cggagactgg ttttgggaag accgttttag gtgctgctct tatctctgaa
     2041 cggaaaacaa aaacaattat tctagtccat aataagcaac tcttagacca atggctagat
     2101 cgcttaaact gctttttgac tttcgaagag gaggaggcta tccgttatac ggcatcaggt
     2161 cgtgaaaagg taatcggcta tgttggacag tacggtggga ctaagaaatg gctgagtaaa
     2221 ctggttgatg tcgttatgat tcaatctcta tttaagttgg aaaatagtca aagtcttttg
     2281 gatgagtatg agatgatgat tgtggatgag tgtcatcatg tctctgcctt gatgtttgaa
     2341 aaagttgttg ctcagtttag agggaagtat ctttacggtt tgacggctac gcctgagcgt
     2401 aagaatggtc atgagcctat tgtttttcag agaattggtg agatactcca tactgctgat
     2461 aagagggaaa cggattttaa acggcaattg caattaagat tcacttcttt tggtcatttg
     2521 gagattgaaa agaccaaagc aagtaatttt atacagctca gtgattggat tgctacagac
     2581 tcagcgagga atcagttgat tctcaaggat attctagccc aagtggcaga aggacggaat
     2641 atcttggttt tagttaatcg aattcaacag atagatgtct ttgaaaagtt attgaaagag
     2701 aaagaggttg atggctgtta cattattagc ggaaaaacca aagtccgaga aagaacgagt
     2761 ttactggaga cgttagaaca gttagataaa gggtttgttt tgttgtctac tggaaaatac
     2821 attggcgaag gttttgactt acctcagttg gacacgctta tcttggcagc acccttttct
     2881 tggaaaaata atttaattca gtatgcaggt cggattcata gaaactacaa ggataagtct
     2941 ttggtgcgta ttttcgatta tgtggatatt catgttcctt atttagaaaa gatgtttcag
     3001 aaacgacaag tagcttatcg aaagatggat tatcgtgtca tcgagggtga ggagaaacaa
     3061 ttcgtttatg ttgatagtag atatgagaag gtgttgagag aggacttagc aggggaaaga
     3121 caggaatgtc tgcttatttt accttatgtg caccagacaa aactgatgaa ttttctaaaa
     3181 gaatttagga ttagtcaaat tgagatatgt ataccagaga cggttgcaaa taaagcatgg
     3241 ctagaccagt tgaagagcca gaaaattaaa gtgtctttta ctcaatcaaa aatagtaacg
     3301 cctattcttt tggtgaataa gactattgtt tggtatggtg caatgccatt attagggaag
     3361 gtagatgaga tgaccatatt acgtttggaa tcagctagta tagtttctga actagtggca
     3421 ggtttacgat agagaaaatt tttaaaaatt tctatgtatg attttcattt ctttagtgag
     3481 actgttgcca ttatcacatt cgaatcacac aaaataaaaa aatttttata agtacttgac
     3541 aaatagattg aaatatcata aaataaaaac ggttacagag ttattaatta tttaagcttc
     3601 atgtcaccat taaaaattga aataaaagga tgttatcact aatacaagtg agcaggaacc
     3661 tatttaatca catcagaaga agtttcttga tgtttttaag taggttcctt ttattttaaa
     3721 agggaaattt tatgatcata aaacgaatac taaaccacaa tgccgtaatt gcgcaaagta
     3781 aaaaagatat cgatattctt ctttttggaa ggggaatagc ttttggaaga aaaactggag
     3841 ataaagtaaa tccaattgat attgagaaaa gtttttttct caaaaataga gataatatga
     3901 cccgttttac agagatgttt attaacgttc ctttggagtt ggtgtacatc accgaaaaaa
     3961 taattaacct aggtaaaata acattgggta ataattttga tgaaattatc tatattaatt
     4021 taacggatca tatttcttcg agcatagaac gttataaaga agggattatt atttcgaatc
     4081 ccctacgctg ggaaatatcg aaatattata aagaagaatt tgaacttggg aaaagggctt
     4141 tacaaataat aaaaaaagag ttaggtattg aacttccaat tgacgaagct gcattcatag
     4201 cgctacattt tgttaatgct aatttagaaa ataattttca agagtcgtat aaaatcactg
     4261 aaataattat gggaattgag aaaatcattc aagatttcta ttgtactgag tttaaccaag
     4321 attctattga ttattataga ttcataactc atataaaatt atttgcccat cgcttggttg
     4381 aaaatacaac ttattgtgac gatgatgatg aagacttgtt agcattaatg aaaaataaat
     4441 atcctagaga atatgaatgt ggtgaacagg tggcaatgtt tatacaaacg gagtacaatt
     4501 atttgctgac ttctagtgaa ttagtttatt tgatggctca tattcgtcgc ttaacaaaaa
     4561 atttagatta atagaatagg agaataccat gagttataaa gatacggtac aaaaaatcct
     4621 cgatgtaatt ggaggtgaaa aaaatgtcaa tagagttacc cattgtgtaa cacgtttaag
     4681 attagaatta aaagatgaaa atttagtcaa tgatgatgat gtgaagaaga taccaggtgt
     4741 aataggtatt atgaaaaaga atggacaata tcaaattata cttggtaatg atgtagctaa
     4801 ttattataaa gaattcgtta aacttggcaa ttttgaatcc gattcagttg ttcaagggca
     4861 caaagggaat attttagaaa gaatcattga gtatatcgct ggttccatga ctccaatcat
     4921 tccagcaatg ttagggggag gtatgctgaa agtcttggta attattttac caatgcttgg
     4981 tatattgcaa tcagattctc agactattgc ttttttgaca ttttttgggg atgctccata
     5041 ttatttctta ccgctgttat tagcttattc tgcatcacaa aaattaaaag taacatctac
     5101 attagctatg tctgtagcag gtgtacttct ccatccaaat tttgttcaaa tggtgcaatc
     5161 agggaatcct cttagtttat ttggtgtacc tgtgacacca gctagttatg gttcgtcagt
     5221 cgttccaatt cttattatgg tttggttgat gaaatatatt gaaaaaatga ttgctaaatt
     5281 aacaccagct gttactaaga gttttttgca acctacgcta gtattattag tatcaagctg
     5341 tattgcctta gttgtagtcg ggcctattgg agtaattgtt ggtgaaggat tatcaaatct
     5401 agttgggcaa atgtatggtg tagctggatg gcttacatta gctattcttg gtgctattat
     5461 gccatttatt gttatgactg gaatgcattg ggcttttgca cctatttttt tggcggcatc
     5521 tattgctact ccagacgtat taattcttcc agcaatgtta gggtcaaact tagctcaagg
     5581 ggctgcttcg atggctgttg cattaaagag taaaaataat aatacaaaac aaattgcttt
     5641 tgcagcaggt ttctcagcct tacttgcagg gattaccgaa cctgcattat atggtgtgac
     5701 tttaaaatat aaaaaaccgc tttatgcagc tatgattggt ggtggattag cgggattatt
     5761 tgcaggtctt actagtgtta aagcgtatct atttgctgtc ccatctttga tagcgttgcc
     5821 tcaatttatt tattctgatg tgccatcaaa tattgtaaat gctttaattg tggcggttat
     5881 ttcggttgtt attacctttg tattagctta tatatttgga atcgatgaag aagagagttc
     5941 tagcaattta gaagttaaag ctggagtttc aaataaaaaa atgatatttt ctcctatatc
     6001 aggagaaatc attccgttaa gcgatgtcca ggataaaaca ttttcagata aactaattgg
     6061 agacggagta gcgattattc caagtgaagg taaggtttat gcaccatttg atgggaaaat
     6121 tacaaatatt tttccgacta agcacgcaat tggattgaag agtgatgagg gtgttgagtt
     6181 actaattcat attggattag atactgttga gctaaaaggt caaggtttta ttagtcatgt
     6241 agaagaagga gacagagttt tcaaaaatca gttgattttt gaaatggact tgaatttaat
     6301 caagactaaa ggctacgaaa cagttacacc agtaattgta acgaatacca atgattttct
     6361 agatgtatta gtattaccta ataatcagac aatcgagcat tctaaggaat tactggtaat
     6421 attataatgg aggaaagtta tgaccatttt tccagatgat tttctttggg gtggagctgt
     6481 tgcagctaat caagtagagg gagcatataa tgaagatggt aagggcttat cagttcagga
     6541 tgtgttaccc aaaggtggat taggagaagc aacagaaaat cctacagaag ataacttaaa
     6601 attgataggt attgattttt atcataaata taaggaagat atatccttgt tttctgaaat
     6661 gggctttaat gtttttcgta cttctattgc atggagtaga atttttccaa aaggagatga
     6721 ggaagaacct aacgaagctg ggttgaaata ttatgatgaa ttgtttgatg aactacatgc
     6781 tcacgggata gaaccacttg taactctttc acactatgag actccattat atttagcaag
     6841 aaaatatcat ggatggattg ataggagaat gattcatttt tatgaaaaat ttgctcgaac
     6901 agttttagaa aggtataaag ataaagttaa atattggctt acatttaacg aagtaaactc
     6961 tgttttggaa ttaccgttta ctagtggagg aatagatata cctaaggaga atctttcgaa
     7021 acaagaatta tatcaagcta tacatcatga attagtcgcc tcgagtttgg ttacaaaaat
     7081 tgctcgtgag attaattcag agtttaaggt ggggtgtatg gtattagcta tgccagctta
     7141 tccaatgact ccaaatccaa aagatgtatg ggcgactcat gagtatgaga atctaaatta
     7201 tctattttca gatgtgcatg ttagaggtta ttatccgaat tatgcaaaaa gatattttaa
     7261 ggaaaatgac attaacatag agtttgcagc tgaagatgca gagttattaa aaaattatac
     7321 tgtagatttt ttatccttta gctattacat gagtgtgact caatctgctc ttccaacaca
     7381 gtataattca ggagaaggga atattattgg tggtttagta aatccttatt tagagtcttc
     7441 tgagtgggga tggcaaattg atccaattgg actacgtata attttaaata gatattatga
     7501 ccgttatcaa attccattat ttatagtgga aaacggatta ggtgctaaag atcaactaat
     7561 aaaagatgaa tttaataact taacagtcca agatgattat agaattcagt atatgaaaga
     7621 acatttattg caagtagctg aagctctaca agatggtgtg gaaattatgg gctatacgtc
     7681 ttggggatgt attgactgcg tttctatgtc cactgcacag ctttctaaga ggtacggtct
     7741 catttatgtt gatcgaaatg atgatggaaa tggcacattc aatcgatata agaaaatgtc
     7801 ctttacttgg tataaaggag tgattgaatc aaatggagaa tccttattca aatagtttgt
     7861 ttattgatga ttagatatta tcacggttaa ggcagcatct cattaaatat gaaataaaga
     7921 ggtttggaag acatcaaatt ctaaacctct ttattttata gggggattaa ttaatttaat
     7981 tttgttacag agagccgatt ataaatagtt aacttaggat agattggtta tatatagtag
     8041 actgaatcta aaatagtacg aaacaattgc taaaacattt atagaaatta attttacttt
     8101 cccaatcgat ttgttttcat cttatttcaa tccgctataa agttagataa gaataagcta
     8161 acaagcagaa gtaagatagg tcttgcaaga atggtaaaaa tctgataaac taagtggtaa
     8221 ttgaatagaa atctgaaaga ctagtagccc aagggaagta tatcagggag gagagccgtg
     8281 actgcaagct ctctatatga aagctgggtg aattcacttg cgcatggatt tagaaatgaa
     8341 ggtctgactt gtcagatgaa aacggatggt accgcgtgtc aacgctccga gtggagtttt
     8401 tggcatgtgg ttttcttttt atctacgaga gactgatgga ggaaatatgt caactattga
     8461 agaacaatta aaagcgcttc gcgaagaaac gctgactagc ttgaagcaga ttactgctgg
     8521 aaatgaaaaa gagatgcaag atttgcgtgt ctctgtcctt ggtaaaaagg gttcgctcac
     8581 tgaaatcctc aaagggatga aagatgtttc tgctgagatg cgtccaatca tcgggaaaca
     8641 cgtcaatgaa gctcgtgatg tcttgacagc tgcttttgaa gaaacagcta agctcttgga
     8701 agaaaagaaa gtcgcggctc aactggctag cgagagtatc gatgtgacgc ttccaggtcg
     8761 tccagttgcg actggtcacc gtcacgtttt gacacaaacc agtgaagaaa tcgaagatat
     8821 cttcatcggt atgggttatc aagtcgtgga tggttttgaa gtggagcaag actactataa
     8881 ctttgaacgt atgaaccttc caaaagacca cccagctcgt gatatgcagg atactttcta
     8941 tatcactgaa gaaatcttgc tccgtaccca cacgtctcca gttcaggcgc gtgctatgga
     9001 tgcccatgat ttttctaaag gtcctttgaa gatgatctcg ccagggcgtg tcttccgtcg
     9061 cgatacggac gatgcgaccc acagtcacca attccaccaa atcgaaggct tggtagttgg
     9121 gaaaaatatc tctatggctg atcttcaagg aacgcttcag ttgattgtcc aaaaaatgtt
     9181 tggtgaagag cgtcaaattc gtttgcgtcc atcttacttc ccattcacag agccatctgt
     9241 tgaggtggat gtttcttgct tcaagtgtgg tggagaaggc tgtaacgtat gtaagaaaac
     9301 aggttggatc gaaattatgg gagccggtat ggttcaccca cgtgtccttg aaatgagtgg
     9361 tatcgatgcg actgtatact ctggttttgc ctttggtctt ggacaagagc gtgtagctat
     9421 gctccgttat ggaatcaacg atatccgtgg attctaccaa ggagatgtcc gcttctcaga
     9481 acagtttaaa taatgattag aaaagtagaa atggcagatg ttgaggtgtt ggctaaaatt
     9541 gccaaacaag cctttcgtga aacctttgcg tatgataata cggaagagca gttacaggaa
     9601 tactttgaag aggcttatag tctgaaaact ttgtcaactg agttgggaaa tcctgactct
     9661 gaaacctatt tcattatgca tgaggaggag atagctggtt ttctcaaagt caactgggga
     9721 agtgctcaaa ctgagagaga attagaggac gcttttgaaa ttcaacgcct ctatgtgcta
     9781 caaaaattcc aaggatttgg actaggtaag caactgtttg aattcgcact tgaacttgct
     9841 acaaaaaata gtttttcttg ggcttggcta ggtgtttggg agcataatac aaaagctcaa
     9901 gcgttttata atcgatatgg ttttgaaaaa tttagccaac atcattttat ggttggtcaa
     9961 aaagtagata cggattggtt actgagaaag aaattaaggt aagaagagga ttcttctatt
    10021 gaaatgatag gaaaggaaaa gaaccagtgt ggcaatggtc attctggaga actaaattat
    10081 gcttatatct tataaatggt taaaagaatt ggtggacatt gatgtgccat cacaagagtt
    10141 ggctgaaaaa atgtcaacta caggaatcga ggtagagggt gtcgaatcac cagctgctgg
    10201 tctctcaaaa attgtcgtcg gtgaggtctt gtcttgcgaa gatgtgccag agactcacct
    10261 ccatgtttgt caggttaacg ttggcgaaga agagcgtcag atcgtttgtg gtgccccaaa
    10321 tgtgcgtgct gggatcaagg tcatggtggc tcttccagga gctcgtatcg ctgataacta
    10381 caaaatcaaa aaaggaaaaa tccgtggttt ggagtcactt ggaatgatct gttcacttgg
    10441 tgaattggga atttctgact cagttgtgcc taaggaattc gcagatggca tccaaatctt
    10501 gcctgaagat gccgtgccag gtgaggaagt cttttcttac ctagacttgg atgatgaaat
    10561 catcgaactt tccatcacac caaaccgtgc agatgccctt tctatgtgtg gagtggctca
    10621 cgaagtggca gccatctatg acaaggcagt caactttaaa gaatttactc taacagaaac
    10681 taatgaagct gcggcagatg ccctttctgt cagcattgag acagacaagg cgccttacta
    10741 tgcagctcgt atcttggaca atgtgaccat cgcaccaagt ccacaatggt tgcaaaatct
    10801 tctcatgaac gaaggaatcc gtcccatcaa taacgtagtg gacgtgacca actacatcct
    10861 gctctatttt ggtcaaccaa tgcatgcctt tgacttggat aactttgaag ggactgacat
    10921 ccgtgtgcgt gaagcgcgtg ctggtgaaaa attggtgacc ttggacggtg aagaacgtga
    10981 cttggacgtg aatgacctag tcatcactgt cgcagacaag ccagtagccc ttgcaggtgt
    11041 catgggtggt caagcaacag aaatctctga aaaatctagt cgtgttgtcc ttgaagctgc
    11101 tgttttcaat ggcaaatcta tccgtaagac aagtggtcgc ctgaaccttc gttctgagtc
    11161 atcttctcgc tttgaaaaag gaattaatgt ggcaacagtt aatgaagccc ttgatgcggc
    11221 agctagcctg attgcggaac ttgcaggtgc gacggtgcgt aagggcatcg tttcagcggg
    11281 tgagcttgat acttcagatg tagaagtttc ttcaaccctt gctgatgtta atcgtgtcct
    11341 cggaactgag ctgtcttatg ctgatgtaga agacgtcttc cgtcgtcttg gctttggtct
    11401 ttctggaaat gcagacagct ttacagtcag agtcccacgt cgtcgttggg atatcacaat
    11461 cgaagctgac ctctttgaag aaattgctcg tatctatggt tatgaccgct tgccaactag
    11521 tctaccaaaa gacgatggta cagcaggtga attgacagcc acacaaaaac tccgccgtca
    11581 agttcgtact attgctgaag gagcaggttt gacagaaatc atcacctata ctctaacaac
    11641 tcctgaaaaa gcagttgagt ttacggctca accaagtaac cttacggaac tcatgtggcc
    11701 aatgactgtg gatcgttcag tcctccgtca aaatatgatt tcaggtatcc ttgataccgt
    11761 tgcctacaac gtggctcgta agaataaaaa cttggccctt tacgagattg gaaaagtctt
    11821 tgaacaaaca ggtaatccaa aagaagaact tccaaatgaa atcaacagtt ttgcctttgc
    11881 cttgacaggc ttggttgctg aaaaagattt ccaaacagca gcagttccag ttgatttctt
    11941 ctatgctaag ggaatccttg aagccctatt tactcgtttg ggactccaag taacctatac
    12001 agcaacatct gaaatcgcta gccttcatcc aggtcgtaca gccgtgattt cattcggtga
    12061 ccaagttctt ggtttccttg gccaagtgca tccagtcact gccaaggctt acgatattcc
    12121 agaaacgtat gtggctgagc ttaacctttc agctatcgaa gctgcgcttc agccagcgac
    12181 tccatttgta gaaatcacca aattcccggc agtcagccgt gacgttgccc ttctcctcaa
    12241 ggcagaagtg actcatcaag aagttgtaga tgctatccaa gctgccggcg tgaaacgttt
    12301 gacagatatc aaactctttg acgtcttctc aggtgagaaa ttgggacttg gtatgaagtc
    12361 aatggcttat agcttgacct tccaaaatcc agaagatagc ttaacggacg aagaagtcgc
    12421 acgctatatg gaaaaaatcc aagcatcgct cgaagaaaaa gtcaatgcag aagtgcgtta
    12481 aacgatagta tgatacaaat tccccctcta gtttatgaac actagagggg tgattttata
    12541 taaaataatt aaaatgtgat ttctgtaaaa atacatctat gatcagagtt ttgaaaactt
    12601 acaatgtcta aatctttaac atagtagtgg tttttaggca ataaaatatg atctattgtt
    12661 gcattaaaaa gttttggact ttggctattc caagttcctc tttcaaaagg tggcagtaca
    12721 tttaatgcgt ccctatgaga gcttattttt gcaagtgctc catgacgcat agttgcatta
    12781 aaatcacctg caataatagc ctttggatat tttgaagcca attgattatg aatgatgttt
    12841 aagtcttgct tccagatttc cattaaacct ggcagaggag gagcagtatg caaggcaatg
    12901 atatctggta tattttgttt tctcgaatgt aatacaattg tcccgaaccg tgttgtatga
    12961 aaagttttag cttctgtata gaaaccataa cttttcttga caatcacagt cacaggagct
    13021 attccactat tggtaggtgg agaagtgaaa atatcatagt tggccataga aagtccaact
    13081 tgatgaaaca atagtttgat tctctggttt tcttgctcac ctctgatatt ggtagctagt
    13141 tcagggaata tagccatatc ggcgtcaaaa tggctaaaaa ttcgctcaat atgttgtgct
    13201 tctatttgat tagcgacgtt ccaagttact aattttaagg ttttagtctt atttgatgca
    13261 gaagaaaggg ttttatctgt tccgaataat agtaatatga gcgatagtaa gagcaaaatg
    13321 ccagagtaaa accgtatttg tttatttttc ttgtaaaaag atagggaaaa aaatacgaaa
    13381 cttaaaatag cagccatagt tgcagtagct aaggggaagg cataaatctg tgccaagata
    13441 ggttgggtag atagccaaat gttaagatta gaagatggga aaaaagaata agttataata
    13501 gggatagaaa agagaatgac cgaaagggca gagagaaatg ttttaatcat tgtgtgagta
    13561 cctcaatttg caatggttgc tcggagctaa tgtgactttg tgactttact tgattgatat
    13621 tctttttctt gtcttcctca tagattaagt acaacccaag taaaaaaagt ccaatttaag
    13681 attgaaatga ttaaaaggca cagaaatgca agacaattat actacaaaag gcaaacattt
    13741 gacaatcgat agccgtcgct taatcgaacg atggaaaaaa gaaggaaaat caaatagaga
    13801 aattgcctct ctacttggaa aagctcctca aactatccat actgaaatca agtatgggac
    13861 agtccgacaa tgtcttggaa aagggcgctt caaagagatt tattctgccg actacgctca
    13921 acagtcttat gaaaataatc gcaagcactc ggtcaagaaa tcaagcttga ctaagaaact
    13981 aaaggaaaag attctccact atcataacca aaaattttcg cctgaaatga tggtgatggc
    14041 taaaggggtt aacgtgggaa tttcaaccat ttactattgg attcatcgtg gaaaattggg
    14101 gttaagcaag caggatctgc tttatcctag aaaaggaaaa gcgcttaaga aataggctag
    14161 caccaacttt aaacccgctg gtcaatctat cgaatcataa ttcgctcatt tttttaaaaa
    14221 tacatagacg taaaaaaaag agggttaagt gatgaagaat agtgctattg ggagtaattg
    14281 gaaggatgtc catctgagct ctttagcaag gaggaaatcc ttgaaagtga tatgcgagtg
    14341 gctatcatga gcgagttgat tgaggctagg tatgaacaag gaatcagtca gaaaaagctg
    14401 gaagaagtca gtggaataag ccagcctgtc atagctagga tggagacagg agagagcagt
    14461 cttcagttgg atacggtctt aaaagttcta accagtctag gaaagacact agcagtcgtt
    14521 ccacttgaac aggggaaaag ttgactagaa tcagttatag tcttttctaa taacaggtca
    14581 tagtcactta taagaattac taataacatg ttttagtatt ctaattggaa tatagctgta
    14641 aagtaagaat tacaaaggat ttgagacgtt tgcttcaagt ctttttcttt tgtccgaaac
    14701 agatatatag tttcaaacta tatcaaaccc taatttttta caattataca gacgctttct
    14761 tttctgttaa gatagtttca acaacaaatt ttggaggaca catcatgtca actacaatca
    14821 tcggtttccc tcgtttgggc gaattccgtg aattaaaatt tacaactgaa aaatacttta
    14881 gaaaagaaat ctcagaagaa gaacttttag cagcagcaaa agacttgcgt gctaaacact
    14941 ggaacattgt caaagaaaaa ggcatcactg aaattccatc aaatgacttt tctcactatg
    15001 ataacttcct agatgcggct ttccttttca acgtggtgcc tgcatccgtt caaaacttgg
    15061 acttgtctga ccttgagcgc tacttcgctt tgggacgtgg ttaccaggga gaaaaagggg
    15121 acgttcgcgc ccttccaatg aagaaatggt tcaacactaa ctaccactac atcgttccta
    15181 aatttgaaaa agacactcaa gtaaaattgg ctggtcacaa gattttcgat gagttccaag
    15241 aagcaaaaga acttggattg aacactcgtc ctgttcttgt aggtccattc actttccttc
    15301 aattgtcaga ctttgaagaa ggcgtgaaag cagacgactt cgtagatagc ttagtggttg
    15361 cttaccaaga agtttttgct aaattggctg aacttggtgc gactcgcatc caattggatg
    15421 aagcggctct tgtaaaagac ttgacagctg aagaaaaagc tctcttcttg aacctttaca
    15481 acaaactctt ggctgacaaa aaaggtcttg aagtcttgct tcaaacttat ttcggtgatg
    15541 ttcgtgacgt atacgctgac cttgtaaact tgccagtaga tgctatcggt cttgactttg
    15601 ttgaaggtaa gaaaactctt gaactcgtta aaggtggctt cccagctgac aagactctct
    15661 atgtaggtat tgtcaatggt aaaaacatct ggcgcaacaa ctacgaaaag agcttggctg
    15721 ttcttgagca aatcccagct gaaaacatcg ttttgacaag ctcttgctca cttcttcatg
    15781 tgccatttac aactgctaat gaagaatttg aaccagcact cttgaaccac tttgcctttg
    15841 cagttgaaaa attggatgaa atccgtgact tggatgctat ccgcaatggt caaggttcag
    15901 aagctcttgc agccaacaaa gaactctttg cgactgagcg tgttggtgaa aatgctgaac
    15961 ttcgtgcgcg tatcgctggc ttgacagacg cagactacac tcgtttgcca gcctttgcag
    16021 aacgtgaagc tatccaagaa gaagctttca aacttccagc tcttccaaca acaacgattg
    16081 gttcattccc tcaaacaaaa gaagttcgtg ccaaacgttt ggcttaccgt aaaggtgaat
    16141 tgtctcaaaa agagtacgat gctttccttg ctgaaacgat tgacgaatgg atcaagtggc
    16201 aagaagatat tgactttgat gtccttgttc acggtgaatt tgagcgtaat gacatggttg
    16261 agtacttcgg tcaaaacttg tcaggatacc tcttctctaa aaatggatgg gtacaatcat
    16321 acggtatgcg tggggtaaaa ccaccaatca tctggggtga tgtcactcgt cttaactcta
    16381 tcactgttaa atggtctagc tatgcacaaa gtcgtacaaa caaacctgtt aaaggtatgt
    16441 tgactggacc tgttaccatc cttaactggt cattcccacg tgaagacatc tctatcaagg
    16501 attcaactct tcaaatcgcc cttgctatca aggatgaagt acttgacctt gaagctgctg
    16561 gtgtgaaaat catccaaatc gacgaggctg ctcttcgtga aaaattgcca ctccgccgta
    16621 gcgactggta cgaagactac cttgactggg ctattcctgc cttccgcttg gtacactcaa
    16681 cagtagcgcc agacacacaa atccacactc acatgtgtta ctcagaattt acagatatca
    16741 tcccagctat cgataacatg gatgcagacg ttatttcctt tgaagctagc cgttcaaacc
    16801 ttgaaatctt ggacgaactc aaagcgaaaa acttccaaac agaagtggga cctggggttt
    16861 acgatatcca ctcacctcgt gtaccaaatg aaggcgaaat cgacaacaca atcgaagcca
    16921 tccttgctaa agtgccaagc aagaaagttt ggatcaaccc tgactgtggt ttgaaaacac
    16981 gtggtatccc agaaacaaaa gaaagcttga tccgccttgt tgaagcagct aaagctgcgc
    17041 gtgagaaatt gtaagattgg atttctctcc atacagtggt tgttcaaaaa gaaatcaact
    17101 agaaaaggtt attacatatg tcacgccaaa caccgtcact ctcatttgaa gtgttccctc
    17161 caaacccagc cgtgggtaat ggtaacatta tttcagcctt gcaggatatg caggagttgg
    17221 ctccccattt tatcagtgta actgccagca ataataaatt taatatcaag gaaacgacgg
    17281 ttcgtttggc tgactttatc caaaatgact tggcgattcc gaccattgcc cacttgccag
    17341 ctatttatct aactaaggac aaggttgctg aaactattgc tgacttggac aaagttgggg
    17401 tgcagaaaat cttggctctt cgtggggata ttattccaga cgtagaacca caaaaggatt
    17461 tccgctatgc aaccgacttg attgagttta tcaaggaaca agcccctcac tttgatatta
    17521 ttggagcttg ctatccagaa gggcatccag attcgccaaa tcagatttca gatattcaaa
    17581 atcttaagaa gaaagtggat gcaggctgtt cgagcctcgt aactcagctc ttctttgata
    17641 atgagcgctt ctatgatttt caggacaagt gtattttggc tgggattgat gttcccattc
    17701 atgcaggaat tatgccaatt ctgaatcgaa atcaggctct ccgactcttg aagacttgtg
    17761 agaatatcca tcttccacgc aaatttaaag ccatcttaga caagtatgag catgaccctg
    17821 agtcgctcag agcagcagga cttgcctatg cagtggacca aatcgtggac ttggtaactc
    17881 aggatgttgc cggtgtgcat ctctatacga tgaataatgc tgatacagca aaatacatcc
    17941 atcaagcaac ccatgccttg tttaatcacc agtctctagg ataataaaag caaaccattc
    18001 ttctcaggtg aggggaatgg ttccttttta atggtaaaga cctcactttt taagaaaaat
    18061 atgataaaat aaattctgta cgtacttgat acaaagatga aggtattaaa taatacaatg
    18121 catttaaact gtaagattta acgacacgag tgagtcaaat cgatgttatt tgacgagaga
    18181 gttagtaaag catttagcta atcgcccgat agcgattagc gtgaaagctt tagatacttt
    18241 agtattaaac ctttctaatg atgtatcttg aaaactgaac ttgcctacaa ctctgtggaa
    18301 aaagataaat ctccctagga gcagtcgctc cgtcgttcga tttcctattt tcctttgagt
    18361 tgcttgacgg cttggtatct tgatgacttg aattttggtc tcatttctta ggaaaaaaga
    18421 taagcttcct agcactcgtc gagtgcgacg tcaccttcct atttttctac agaaatgttc
    18481 ggcaagccga accgtccaaa atatcttgtg taattgaaca cggccgaaaa gctgtgtaaa
    18541 aaagataaac tgtcttgtct tcatcgaaga cttcgtcagt ttcctatttt tactttgctt
    18601 ttgacgtcct tggtatcttg atctttgtag gcaaggcgta taatttcatc aatccaaagg
    18661 ggattaaaat ggcaaaacaa gtgtttcaaa cgacttttgc gggtcgtgag ttaattgtag
    18721 aaactggtca ggttgctaag caagcaaatg gctctgttgt cgtacgttac ggtgagtcaa
    18781 ctgtcttgac tgctgccgtt atgtctaaga aaatggcaac tggggatttc ttcccactcc
    18841 aagtcaacta cgaagaaaaa atgtatgcgg ctgggaagtt tcctggtggc tttatgaaac
    18901 gtgaaggacg tccttcaaca gatgcgacct tgacagcgcg tttgattgac cgtccgattc
    18961 gtcctatgtt tgcggaaggt ttccgtaatg aagtccaagt catcaataca gtgctttctt
    19021 atgatgaaaa tgcatctgca ccaatggctg ctatgtttgg ttcatctttg gcactgtcta
    19081 tttcagatat tccatttgac ggaccaattg ctggggtaca agtgggatat gtagatggcc
    19141 aaatcatcat caacccaagt caagaacaag cagagcaatc tcttcttgaa ttgacagtag
    19201 ctggaaccaa gcacgctatc aacatggtag agtctggtgc caaagaattg tcagaagaaa
    19261 tcatgttgga agcgcttctt aaagggcacg aagctgtcaa agaattgatt gccttccaag
    19321 aagaaatcgt tgctgctgtc ggtaaagaaa aagcagaagt ggaattgctt cacgtggatg
    19381 ctgaattgca agctgaaatc attgcagcct acaacagtga cctccaaaag gcagttcaag
    19441 tagaagagaa attggcccgt gaagctgcaa ctcaagcagt gaaagaccaa gtgactgccg
    19501 tttacgaaga aaaatatgcg aaccacgaag aatttgaccg tattatgcgt gatgtggctg
    19561 aaatcttgga acaaatggaa cacgcagaag tgcgacgttt aattacagaa gacaaggtgc
    19621 gtcctgatgg tcgtaaggtc gatgaaatcc gtcctttgga tgcggttgtt gacttccttc
    19681 ctcgtgtaca tggttcaggt ctctttactc gtgggcaaac tcaagctctt tcagtcttga
    19741 ccttggctcc gatgggagaa actcaaatca ttgatggttt ggatccagag tacaagaaac
    19801 gctttatgca ccactataac ttccctcaat attctgtagg ggaaacaggt cgttacggtg
    19861 cgccaggtcg tcgtgaaatc ggtcacggtg cccttggtga gcgtgctctt gctcaagtct
    19921 tgccaagctt ggaagaattc ccatacgcta tccgtctagt agcagaagtt ttggaatcaa
    19981 acggttcttc atctcaagct tctatctgtg cgggaactct tgcccttatg actggtggtg
    20041 tgccaatcaa ggcgccagta gctggtattg ctatgggact tatctcagat ggaaataact
    20101 acacagtatt gacagatatc caaggtttgg aagatcactt tggagatatg gacttcaagg
    20161 ttgcaggtac tcgtgatggg attacagccc ttcaaatgga tatcaagatt caagggatta
    20221 ctgcagaaat cttgacggag gctcttgctc aagccaagaa agcgcgtttt gaaatccttg
    20281 atgtcattga agcaaccatt ccagaagttc gtccagaatt ggctccaact gctccgaaaa
    20341 ttgatacgat caagattgat gtggacaaga ttaagattgt catcggtaag ggtggagaaa
    20401 ccatcgacaa gattatcgct gaaacaggtg ttaagattga tatagacgaa gaaggaaatg
    20461 tgtctatcta ctctagtgac caagatgcta ttaaccgtgc caaagaaatt attgctggtt
    20521 tggttcgtga agccaaagtg gatgaagttt accgtgctaa agtcgttcgt atcgagaaat
    20581 ttggtgcctt tgttaacctc tttgataaga cagatgccct tgttcatatc tctgagatgg
    20641 cttggactcg taccaatcgt gtagaggatt tggtagaaat cggggatgaa gttgatgtta
    20701 aggttatcaa gattgatgaa aaaggccgta tcgatgcctc tatgaaggct cttctacctc
    20761 gtccgccaaa acctgagcat gatgaaaaag gtgaaaagtc tgagcgccct caccgcccac
    20821 gtcatcacaa ggatcacaaa cctaagaaag aatttacaga aacaccaaaa gattcagagt
    20881 aagaaaagga gaaatgtatg gggtggtggc acgaaaccat tgatatcgta aaagaaaatg
    20941 atccagcggc ccgcaccact ttggaggttt tgctgactta tccaggtgtc aaggccttgg
    21001 cggcccaccg tctctcgcat tttctctgga agtacgactt caaactctta gctcgtatgc
    21061 acagtcagtt ttggcgcttt tggactcaga ttgagattca tccaggagcc cagattgatt
    21121 caggtgtctt tattgaccat ggttctggtc tggtgattgg agagacagcg attgttgaaa
    21181 aaggcgttct tctctatcac ggagtgactc tcgggggaac agggaaagac tgtggcaaac
    21241 gccatccgac tgtacgaaag ggagccctca tatcagccca tgcccaagtt atcgggcctg
    21301 tggaaattgg tgaaaatgcc aaggtcggtg ctgcagcagt tgtcgtagca gatgtaccta
    21361 gtgatgtgac ggttgtcggt attccggcca agattgtccg tcttcatggt aagaaagatg
    21421 agcctgttat tcacgaagtc gaagaaaaac gggagtatta tgtcaataaa ctcgagcagg
    21481 ctaaagatgc cagtcacaga tcgtctggtt tgtagaggat acctatatga ttcaagcaag
    21541 aaacaagtta agccaagagg agctatctga ggcgaaaaaa gtaattaact gttgccaaaa
    21601 ctatgacggt acctatcgcg atccctatct ctctaacatg cttaattttg acccaaacat
    21661 gcccgccttt ttcctttatt atgaaaaagg caaacttgtt ggtttattaa ctgtctatgc
    21721 agatgaccaa gatgtagaag tgacgatatt ggttcatcca ggtcatcgcc gtcaggggat
    21781 tgcgcgtgca ttgtttacta gttttgagag agaaacagct tctttcccca ttcggtcagt
    21841 cacttttcag acagaacgta tttttttaga gaatcatcct gactttgcca gtaactgggg
    21901 actaatcgag gatgaagaga cagaaacttg gttaggtaag gatagaagac cttatccgtt
    21961 agcaaatgtt tcccatcttg aagttttgtt agcagatagt tcgtatcagg atcaaattag
    22021 tcaattaaaa tttcaggcat tttcagagga acatgaatcg agagaagttg tggatagata
    22081 tgtcgctgaa gctctgaagg atccagaaag tcgcttatat attttgttaa aagacggtca
    22141 ggttattggg acttgcacgg tagatttatc gactaatacg aattacttct acggtttagc
    22201 aatatcggaa cctgaacgtg ggaaaggcta tggaagctac ttagcaaaat ccctcgtcaa
    22261 ccaactaatt gagcaaaatg acaaggaatt tcagattgca gtggaagata gcaatgtagg
    22321 tgccaaacgt ttgtatgaaa aaattggctt tgtcaaacag acacaggtgg tttatctgaa
    22381 tgagaaagga gcaagggatt ccgaagtgta gagacattcg gactgaaatt tacttgagct
    22441 cttagtttag tgatgaaact aacagttctt ggattttagc tttcctgatt atgatttatg
    22501 attaaaatct atgacaccat gtctcgtgat ttgcgagaat ttgtcccgat tgaggacggc
    22561 aagatcaaga tgtatgtttg tgggccaacc gtgtacaact atattcacgt gggaaacgcc
    22621 cgttcgacgg tagcttttga tacgattcgt cgctattttg agtaccgtgg gtataaggtt
    22681 gcctatattt ccaattttac agatgtggat gataagatta tcaaccgtgc cagggaagaa
    22741 ggcatcacac ctcaggaggt tgcggataag tacatcgctg cctttcgtga ggatgtgacg
    22801 gccttaggcg tgaaacctgc gactcgccat ccgcgtgtag tggagtttat ggcagacatc
    22861 atccgttttg tggaagactt gatcgagaaa ggctttgcct atgagagtca aggggatgtc
    22921 tatttccgtg tagaaaaatc ccacaactat gctaaattgg ctaataaaac cttggaagat
    22981 ttggagctag gtgcttcagg tcgtaccgat gaagaaacgg ctcgtaagga aaatcctgta
    23041 gactttgctc tatggaaatc ttccaaacca ggtgagattt cttgggatag tccttgggga
    23101 cctggacgtc cgggttggca tattgagtgt tcagtcatgt cgacagagat tttaggtgat
    23161 accattgata tccacggtgg tggagctgac ctagagtttc cacaccacac caatgaaatt
    23221 gcccaatctg aagctaaaac aggcaaggct tttgctaact actggatgca caatggcttt
    23281 gtcaatatcg acaatgtcaa aatgtctaag tccttgggta acttcattac ggtacacgat
    23341 gcccttaaaa ctcttgatgg gcaagtactt cgtttcttct ttgcgaccca gcactaccgt
    23401 aagcctatca acttcacgga aaaggcagtt cgcgatgccg agaccaatct caagtatctg
    23461 aagaatactt acgaacaacc atttacagga aacgtggatg cccaagagtt acaaaacttt
    23521 aaagataagt ttgtagcagc tatggatgaa gattttaatg ctgccaacgg tatcacagtt
    23581 gtctttgaaa tggccaaatg gatcaactca gggaactatg atgcaagtgt caagcaagct
    23641 cttgcagata tgttagaaat ttttggaatt gtctttgttg aggaagtttt ggatgcagag
    23701 attgaagact tgattcaaaa acgccaagag gcgcgtgcca atcgtgactt tgagacagca
    23761 gaccaaatcc gtgaccaatt ggttactcaa ggaattaagc tccttgatac caaggatgga
    23821 gtgaggtgga cacgtgattg atgtcaatct cattaacggg attgcgctag cctttgaggg
    23881 agatgcggtg tattctatgt atattcgccg ccacctcatc ctcaaaggta tgaccaagcc
    23941 caataaactc caccaagagg cgaccaagta cgtgtcagcc aaggctcagg ctcgtctgat
    24001 tgctctcatg ttggaggagc agatcctaac ggaaaaagaa gaagaaatct acaaacgtgg
    24061 ccgcaatacc aatagtcaca caaaggctaa aaatgcagat gtcgtgactt atcgtatgtc
    24121 cacgggattt gaagcagtta tgggctatct ccatatgact gagaatctgg aacgtcttga
    24181 gagtttggtt tcatggtgca tccaaaaagt ggagggctag aacatgaggg caaaagaact
    24241 acaagactgg tttcctgagg ctcggatttc agaccaacca gtagagaaag agggctatct
    24301 cacgcttcct ttagcttctc agcagtggat tttgctggag gaagctgggc tcagcgagcg
    24361 tgaaaagcag ttggttgccc ttttgaccca gcaggagcag gctcgttcgc taaacccttg
    24421 gtattcctat ctggttgagg gcaagggaca ggcaccgcaa gtttttaaaa agattcagtt
    24481 ggtttattgc catctttctt attttcagca ggaaaatctg acttcttggc tagatatgat
    24541 gcggactctt tttccgaatt gtcagacagt gctacaggtc ggagctcagg attatgtttt
    24601 cgtgcttcaa caagacaaat acacttctgt aagagatatt ttgagtgata cgattgaagc
    24661 ggttgagtat gactttggac ttcgtctttc tatcatgttg ggtcaggttt ggtctcagac
    24721 aggacatcaa gccctatcag acttaatcaa agctgagcgg gatttgttca agacatggtg
    24781 gcgtcagggt caccaaggtg ttcatacttt ttctcagctc tatctttgga gtatgggaga
    24841 aagactcgtg gacttgaagc caattaaaga atgtctacac cagatgattt tggatcaaga
    24901 tcagattcag gaaatcattc tctctctttg ggaaaatagt gctgttctca ctaaaacagc
    24961 ccagcaactc tatctgcacc gcaattctct ccaatacaag attgataaat gggaagagtt
    25021 gacagggctt cagttgaaag agttgaccga cctgaccttg tgttatcaat tgattttagg
    25081 ttctttgtca actatagttg gtttgtaaag aagttaatat ggctctttgt caactgtagt
    25141 gggttgaaaa aaagctaagc tcgagaaagg acaaattttg tcctttcttt tttgatattc
    25201 agagcgataa aaatccgttt tttgaagttt tcaaagtttc gaaaaccaa
//
LOCUS       DAVMMR010000029        24872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMMR010000029 DAVMMR010000000
VERSION     DAVMMR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.029"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            210..623
                     /gene="rpsL"
                     /locus_tag="VYS74_001156"
     CDS             210..623
                     /gene="rpsL"
                     /locus_tag="VYS74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV3789204.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            643..1113
                     /gene="rpsG"
                     /locus_tag="VYS74_001157"
     CDS             643..1113
                     /gene="rpsG"
                     /locus_tag="VYS74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV3789205.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1538..3619
                     /gene="fusA"
                     /locus_tag="VYS74_001158"
     CDS             1538..3619
                     /gene="fusA"
                     /locus_tag="VYS74_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV3789206.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3723..8114
                     /locus_tag="VYS74_001159"
     CDS             3723..8114
                     /locus_tag="VYS74_001159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV3789207.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLIMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8216..8479
                     /locus_tag="VYS74_001160"
     CDS             8216..8479
                     /locus_tag="VYS74_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV3789208.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8472..8750
                     /locus_tag="VYS74_001161"
     CDS             8472..8750
                     /locus_tag="VYS74_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV3789209.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8771..8923
                     /locus_tag="VYS74_001162"
     CDS             8771..8923
                     /locus_tag="VYS74_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789210.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8945..10186
                     /locus_tag="VYS74_001163"
     CDS             8945..10186
                     /locus_tag="VYS74_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV3789211.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10196..10426
                     /locus_tag="VYS74_001164"
     CDS             10196..10426
                     /locus_tag="VYS74_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3789212.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10694..11416
                     /locus_tag="VYS74_001165"
     CDS             10694..11416
                     /locus_tag="VYS74_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3789213.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11633..12967
                     /gene="pepC"
                     /locus_tag="VYS74_001166"
     CDS             11633..12967
                     /gene="pepC"
                     /locus_tag="VYS74_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV3789214.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(13023..13934)
                     /locus_tag="VYS74_001167"
     CDS             complement(13023..13934)
                     /locus_tag="VYS74_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3789215.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13958..14761)
                     /locus_tag="VYS74_001168"
     CDS             complement(13958..14761)
                     /locus_tag="VYS74_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV3789216.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14789..15778)
                     /gene="manX"
                     /locus_tag="VYS74_001169"
     CDS             complement(14789..15778)
                     /gene="manX"
                     /locus_tag="VYS74_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV3789217.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(16011..17030)
                     /gene="adhP"
                     /locus_tag="VYS74_001170"
     CDS             complement(16011..17030)
                     /gene="adhP"
                     /locus_tag="VYS74_001170"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV3789218.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17369..18181)
                     /locus_tag="VYS74_001171"
     CDS             complement(17369..18181)
                     /locus_tag="VYS74_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3789219.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18371..19789
                     /locus_tag="VYS74_001172"
     CDS             18371..19789
                     /locus_tag="VYS74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV3789220.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIYWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19842..20549
                     /locus_tag="VYS74_001173"
     CDS             19842..20549
                     /locus_tag="VYS74_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3789221.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            20644..21618
                     /gene="folP"
                     /locus_tag="VYS74_001174"
     CDS             20644..21618
                     /gene="folP"
                     /locus_tag="VYS74_001174"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV3789222.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            21620..22942
                     /locus_tag="VYS74_001175"
     CDS             21620..22942
                     /locus_tag="VYS74_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3789223.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            22923..23477
                     /gene="folE"
                     /locus_tag="VYS74_001176"
     CDS             22923..23477
                     /gene="folE"
                     /locus_tag="VYS74_001176"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV3789224.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            23520..24332
                     /gene="folK"
                     /locus_tag="VYS74_001177"
     CDS             23520..24332
                     /gene="folK"
                     /locus_tag="VYS74_001177"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV3789225.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(24377..24694)
                     /locus_tag="VYS74_001178"
     CDS             complement(24377..24694)
                     /locus_tag="VYS74_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV3789226.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT         7240 a         4844 c         5753 g         7035 t
ORIGIN      
        1 cagaaattga tctctaacat taccaataaa aaagaaaaat atcttgacaa gcaagggaaa
       61 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
      121 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
      181 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
      241 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
      301 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
      361 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
      421 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
      481 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
      541 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
      601 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
      661 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
      721 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
      781 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
      841 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
      901 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
      961 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
     1021 gctgctaaca acactggtgc agctgttaag aaacgtgaag atactcaccg tatggctgaa
     1081 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaaat
     1141 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
     1201 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
     1261 gtgaaaatag gaaactgacg cagtattcga tgaatacaag gagttttatc tttttcacgc
     1321 agcatcccgt tccagctcat atcggctaac taactttagc ccgggttcaa attagctaaa
     1381 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
     1441 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
     1501 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
     1561 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
     1621 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
     1681 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
     1741 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
     1801 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
     1861 acaatcaggt gttgagcctc aaactgaaac agtttggcgt caagcaactg agtacggagt
     1921 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
     1981 aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
     2041 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
     2101 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
     2161 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
     2221 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
     2281 tatcaacgtt gaattcttcc cagtattgtg tggttcagcc ttcaaaaaca aaggtgttca
     2341 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
     2401 aggtattaac ccagatacag acgctgaaga aattcgtcca gcatctgacg aagagccatt
     2461 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
     2521 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
     2581 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
     2641 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
     2701 gacagatgaa aaagctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
     2761 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
     2821 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
     2881 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
     2941 caaagttgaa gcgaacgtag gtgctcctca agtatcttac cgtgaaacat tccgcgcttc
     3001 tactcaagca cgcggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
     3061 atggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
     3121 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
     3181 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
     3241 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
     3301 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
     3361 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     3421 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
     3481 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
     3541 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
     3601 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
     3661 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
     3721 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
     3781 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
     3841 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
     3901 agaaaggttt gagcgaagaa ttttctaaga caggcaataa agctgttttt gaaattaagg
     3961 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
     4021 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
     4081 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
     4141 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttccaact tttaactgtc
     4201 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
     4261 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
     4321 tggcacctcc tccagcggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
     4381 ctaaacccaa gctggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
     4441 atcgtctggt atttgaaggg gttgtttttg atgtggaaca aaaagtgact agaacaggtc
     4501 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
     4561 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
     4621 ttcgagggaa tgtggagatg aataacttca cacgcgattt gattatgaac gtacaggatc
     4681 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
     4741 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
     4801 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
     4861 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
     4921 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
     4981 tatcagaagc aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
     5041 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
     5101 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
     5161 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
     5221 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
     5281 ctaattatga gcgtcatggt cttccaaaga ttagtcagcc agttattgat acgctggagt
     5341 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
     5401 ttggtgtggc cttggaacat catcacatgg ccaactacga tgcggaagcg actggtcgtc
     5461 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
     5521 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
     5581 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
     5641 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
     5701 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
     5761 gtgtggatgc ggcggttgag gtggccaagt attatgactt tatcgaggtc atgccaccgg
     5821 ctatctatgc gcccttgatt gctaaagagc aggtcaagga tatggaggaa ctccagacca
     5881 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttttg gctacgggaa
     5941 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
     6001 gacagggtgc gatgattaac cgaactatcg gtcatggtga acatgcccaa ccagcaccac
     6061 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
     6121 aggaactggc tcgtaaactg gttattgaaa acaccaatgc cttggcagaa atatttgaac
     6181 ccgttgaagt cgttaagggt gacttgtata cgcctttcat cgacaaggct gaagaaacag
     6241 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
     6301 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
     6361 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
     6421 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg aattacggag gtcaatcctc
     6481 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
     6541 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
     6601 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
     6661 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
     6721 tctttggtga agaatatgcc ttccgtgcgg gaacagttgg tacggtagct gccaagactg
     6781 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagaag
     6841 tagaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
     6901 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
     6961 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
     7021 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
     7081 atttgtctgg tattgaccct aataaaattc ctatggatga cgaaggcgtg atggcactct
     7141 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
     7201 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
     7261 caacctttgc ggaattgctt cagctgtctg gtctgtccca cggtactgat gtttggttgg
     7321 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
     7381 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
     7441 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatggct
     7501 atatcgaagc aatgaaggct aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
     7561 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
     7621 cttacttcaa ggttcaccat cctatttatt actactgtgc ttacttctcc attcgtgcta
     7681 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
     7741 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
     7801 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggtaaa ttagatctct
     7861 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
     7921 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
     7981 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
     8041 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
     8101 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
     8161 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaagagggtg agagtatgtc
     8221 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
     8281 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
     8341 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagcgtt
     8401 aacggattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
     8461 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
     8521 ctatgaagcg tggtttgaag gcagaattat tagaagaagt tttgaatttt ctggttcaag
     8581 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
     8641 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
     8701 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
     8761 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
     8821 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
     8881 tattgcttta gcaggaatct tttctgcttt gaaagaaaaa taaaaataga aaagagaaaa
     8941 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
     9001 gaacggaatt aacgtgcaac ctggtcacac tttggctctc tctattgatg tggagcaacg
     9061 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
     9121 tcagtggaca gatgatgtga ttaaccgtga gaaattcctc catgccccga tggagcgttt
     9181 ggacaatgtg ccagaataca agattgctga gatgaactat cttttggaga acaaggctag
     9241 ccgtcttggg gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
     9301 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
     9361 taacaaggtt agctggactg tagcagccgc tgctggactt gagtgggcta agaaagtctt
     9421 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggatcaaa tcttcaaaac
     9481 ttgtcgtgtc tacgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
     9541 gagcaaggcc gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
     9601 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
     9661 tgcacagggc gaagaattct tgccaaatat gccgacagaa gaggtcttca cagcgcctga
     9721 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
     9781 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
     9841 gggtgatcag gttatgaaag accttgtctt tgaaaatgcg ggtgcgcgtg ccttgggtga
     9901 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tttttaacac
     9961 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    10021 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    10081 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    10141 tggaacgcgg gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    10201 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    10261 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    10321 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    10381 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatctga
    10441 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcactgc
    10501 tttgaggttg cagatagaac tgacgaagtc agctaaaaac actgttttga ggttgcagat
    10561 agaactgacg aagtcagtaa catatatacg gcaaggcgac gttgacgcgg tttgaagaga
    10621 tttttgaaga gtatgaaacg aaaaggaggt tggtcattgt actagcctcc ttttgatatg
    10681 atataatagt tctatgagat tagataaatt tttagttgcc tgtgcggtgg gaagtcggac
    10741 tgaggtcaaa aacttgctca aggctgggcg cgtgactgta aatggtaaaa aagaaaaatc
    10801 agctaaattg cagattgatg aaaaaataga tgagattcgc tttgatgggc aagtgttgga
    10861 gtatgaagag tttgtctact acatgatgaa caagcccaaa ggagttatct cagcgactga
    10921 ggatcccaag cacagaaccg ttctggactt gttggatgac ttggcgcgga gtaaggaagt
    10981 ttttccagta ggacgcttgg atattgacac gcatggtctt ttgctcttga ccaataatgg
    11041 tcagctggct catgttcttc tttcgcccaa gcgtcatgtg gacaagactt atcaggctca
    11101 ggtcaaggga attatgaccc aagaagatgt ggagacattt atcaagggca ttccgctcaa
    11161 agactttacc tgtcagcccg ctagactgga gattctttcc acagatgcag aaaagaatca
    11221 gagccaaatc cgtgtgacca ttgcagaagg gaagtttcat cagatcaagc gtatggtggg
    11281 ctactgtggc aaggaagtgg tggacttgca acgattgact atgggaactt tagtattaga
    11341 tgagaacctg gaacgagggg aatggcgtcg cttgaccaag gaagaattag aaattctccg
    11401 cgctaatatt atttaattta gtttaatact caatgaaaat caaagagcaa actaggaaac
    11461 tagccgcagg ctgtacttga gtacggcaag gcgacgttga cgtggtttga agagattttc
    11521 gaagagtata aattcgaaaa aggtgaggga gaatgtcctt accttttatg ttttttagtt
    11581 gcttcctttg tgaaaagagt tataatagac tgtagaataa aaggaggaat ctatgaacgc
    11641 gattcaagaa tcatttactg ataaactatt tgccaactat gaagcaaatg taaaatacca
    11701 agcgattgaa aatgctgcca gccacaacgg aatttttgca gctctggaac gtcgccaaag
    11761 ccatgtagac aacacacctg ttttctcatt ggatttaacc aaggacaagg tcactaacca
    11821 gaaagcgtct ggtcgttgct ggatgtttgc ggctctcaac accttccgcc acaaactcat
    11881 ctcgcaatac aaattggaga actttgagtt gtcacaagcc cacactttct tctgggataa
    11941 gtatgagaaa tcaaactggt tcttggagca agtcattgcg acttcagacc aagaattgac
    12001 tagccgcaag gttagcttct tactccaaac acctcaacaa gatggtggtc aatgggatat
    12061 ggtcgtttcc ctctttgaaa aatacggtgt cgtgcctaag tcagtttatc ctgagtctgt
    12121 ttcatctagc agcagtcgtg agctaaatgc gatccttaat aaattgcttc gtcaagatgc
    12181 tcaaatcttg cgtgatttgc ttgtttctgg tgcagatcaa gtgactgttc aagctaagaa
    12241 agaagacctc ttgcaagaaa tctttaactt tcttgctatg tcattaggac ttccaccacg
    12301 caagtttgac tttgcttatc gcgataaaga taacaactat aaaagtgaaa aaggaatcac
    12361 accacaagag ttctacaaga aatatgtcaa tcttccttta gaagactacg tttctgttat
    12421 caatgctcca actgctgata aaccttacgg aaaatcttac acagttgaga tgttggggaa
    12481 tgtggttggt agccgtgcag ttcgttacat caacgttcca atggagcgct tgaaagaatt
    12541 ggcgattgcc caaatgcaag caggtgagac tgtttggttt ggttctgatg tcggccagct
    12601 cagtaaccgt aaggctggaa tccttgcgac agatgtttat gactttgaat caagcatgga
    12661 cattaaactt actcaagaca aggctggacg tttggactat agcgaaagct tgatgaccca
    12721 cgccatggtc ttgacaggtg ttgacttgga cgaaaatggt aaatcaacca agtggaaggt
    12781 tgaaaactca tggggagaca aggtcggtac agatggttac tttgttgcct cagacgcttg
    12841 gatggacgaa tacacatacc aaatcgttgt tcgtaaggaa ttgctgacag cagaagaaca
    12901 agctgcctat ggagcagaac caattgtact tgcaccatgg gatccaatgg gagccttggc
    12961 taaataaaag catagaaaaa aggaatcaga ttttagaacc tggttccttt ttagttgctt
    13021 gattacatga tgtgaagaac atgtgccaca atacccactg cgaagagggc aaggataata
    13081 gtgattggag atactttttt cttaagtaag tacatgcaaa gtaaagtaag gagtagtcct
    13141 gataatccag gaatcaacat atccaagttt tgttggaaag tagtaacttt ttcaggagtt
    13201 tgagacaatc cttgtcctac ttgtgcgaat gcttcttgga tacctttaga cccttctggc
    13261 aatttatccc aatggatata agccttttca tctagttgaa ctttagaaac atcgaaagca
    13321 aatttaatat ttacccagcg ttgaacaagg acagcaagaa tgaacatccc aaggatagaa
    13381 gctcctttag tgatatcttg aaggatacca ccagacatat ctttagtgat ttctgatcca
    13441 gccttgtatc caatctcttg aacataccac aagaatgaca tacgaatcaa gttccatgca
    13501 acaaagaaga ggagtggccc caagatattg ccagtaaggg caagtgaagc accgagagat
    13561 ccaaggattg ggcgtactgt aaaccagaat actgggtcac cgataccagc aagaggtccc
    13621 atcataccga ttttaacccc ttggatagca gcgtcatcga tttccacacc gttagcacgt
    13681 tcttcttcaa gcgcaagagt aacccccatg actggagcag ctacgtatgg atgagtgttg
    13741 aagaactcaa ggtgacgctc aagagcagcg atttgatctt cttttttagt atagagtttt
    13801 ttgatagctg gaatgagtgt ataagcccag cccaagtttt gcatccgttc aaagttccaa
    13861 gacccttgta agaaggttga acgccaccaa acttttttac gatctgattt agttaattga
    13921 agtttttcag tcatgatgtt ttcagtcctt tcttatctta gtagtcttct aggatatcgc
    13981 cgattgggtc gttagaagtt gcggctcctc cgccaccatt tccaccagtt ttagaaaggt
    14041 gaaggtagat aagagcgata gcaacaccga tagcaccgaa tccgattaga gtaatatctg
    14101 acacagcagc gagaacgaaa ccaagagcga agaatggcca tacttcacga gttgccatca
    14161 tgttgataac catggcgtaa ccaacggcaa cgaccatacc accaccgata gccataccat
    14221 ctttgagcca gtctggcatg gcactaagga tactttgtac agtttcagtt ggtaccataa
    14281 ggagaagagc tgcaggaagc gcgatacgaa gtccttggaa gagtagcgca atgaaatgcg
    14341 cacgctccac agcgccgaag tcaccttttt tagcggcagc atctgcagta tgaaccaaac
    14401 caactgaaat tgtacgaaca atcattgtca agaaaagtcc agctacggca agagggatag
    14461 caaccgcttg ggcaacaccg ataccagtct tggtaaagtc accaccaaga accatgataa
    14521 tggcagcagc gacagaagca agtgcagcat caggagcgat agcagcaccg atatttgacc
    14581 aaccaagggc aatcatttga agcgatccac cgaggataat ccctgcttcc aagtgacctg
    14641 ttacaagccc aataagggta caggctacaa gtggttggtg aaattggaac tggtcgagga
    14701 tgccttcaag acctgcaaag aaggctacaa cgactactaa aaccatagaa ataatagaca
    14761 tgtttaaaat cctttcataa ataatggctt atttgacatt ggctttgtta atcaagtcaa
    14821 acaaatcttt tttagaatca tttggtactt tacggacatc aaattcaaca cccaagtcac
    14881 gcattttttc aaatgtagca acatcttctt tgtccataga caaaacggta ttgaccaatg
    14941 ttttacctgt tgagtgagcc atagaaccaa cgttaagagt cttgattggc acgccgcctt
    15001 cgatggcacg aagggcatct tgaggtgttt caaacaagat aagggcatgt gtttctccaa
    15061 aacgtgggtc ttttgaaatc tcaatcagtt tttgaattgg aaccacgtta gccttgacat
    15121 tacctggagc tgcttgttta atcaattctt tacgaaggtc gtctttagcc acgttatctg
    15181 aagcaacgat gatacgattt gcttttgaat ctggagtcca agcagttgca acctgaccgt
    15241 gaagtagacg tgtgtcaaga cgggcaagat tgattttcaa tttaccgtct ccgataacag
    15301 ttccttctgg gatagcagtt tgggcaactg gagcagctgc agcgcttgca acttcttcaa
    15361 ctggatttag ctcttctgga agagctttga tgccatcttt ggcttcttta atgatattag
    15421 cagcgacttt ttctacacct gcagcagcgt ccatgaggcg ctctgtgtag gcttgaatca
    15481 acatcggtaa gttaagtcct gtgatgatgg caaacttacg ctcaggattt tctcccatca
    15541 cgcgactagc ttggttaaat ggagaaccac tccaaaggtc agccaaaact agaacctcat
    15601 cttctgcgtc aaatgcagca acagcgttat taaacttagc gtatagatca tcaggacctt
    15661 catttggcat aaaggtcaca acttgaacct tttcttgttc accaaagatc atagatcctg
    15721 actgatgaat acccgcagca aattcgccgt ggctcgcaat aatgattccg atactcatta
    15781 ttgtcattcc tccttttttg ttttagtttt catttaagaa aactttaaga ctgtttaagt
    15841 ataaaccgtt ttcataaaaa aagcaactat ttatcataaa aaattaaaaa atttttattt
    15901 ttgtatgttg atatgttgag tttttgagga gtaatcttga attcttattt aaaatttaat
    15961 ataaaaaagt tggaagaagc ttccaacttt taaaataggt ttctattaaa ttagtgggtg
    16021 aagtcgagta ccatacgtcc ttggatttgg cctttttcca tttcgtcgaa aatggcaaca
    16081 gcatcttcta ctggacgttt ttgaacaact gggactacca gaccttctgc accaaattgg
    16141 aaggcttctt ctaagtcttt acgagttccg acaagagaac cgatgacttg gattccatcg
    16201 aggactgttt taacgatgct gagttccatc atttcagaag gaagaccaac agcgacgacg
    16261 cgaccaccag cacgaatgga gtcaacagcc tggttgaagg caactttaga cacagcagtt
    16321 acgacagctg aatgagctcc tccatcagtt ttttctttaa tgagtccagc tacatcttca
    16381 acttcgaggc cgttaatcac aatgtcagcg cctacttctt ttgcaagggt aagtttgtca
    16441 ttgttgatat cgactgcgat aacatgagca ttgaatactt ttttagcgta ttgaacagcg
    16501 aggttaccaa gtccaccagc accgtaaaga acaacccatt ggcctggttc aacttttgct
    16561 tctttgatag ctttataggt tgttactcca gcacatgtga tagaagaagc ttgggctgga
    16621 tcaagtccgt caggaacttt gacagcatag tcagcagtta cgatacattg ttcagccata
    16681 ccaccgtcta ctgagtagcc agcatttttc actgtacggc aaagggtttc gcgaccagtt
    16741 gtacagtatt cgcaagtgcc acatccttca aagaaccaag caacgctgac gcggtcaccg
    16801 actttaaggc ttttcacatc tggagcaatc tctttaacga taccgatacc ttcgtgccca
    16861 agaacacgtc ctgggacttg accaaagtca ccatgagcaa cgtggaggtc ggtgtggcaa
    16921 acgccacagt attcaacttc tacaagtgct tccccagttt caagtggacg gagtactttt
    16981 tcttcaatag caacaccagt gctttctgga tttacaacaa cagctttcat agctatcctc
    17041 cttgatattg accgagaaca gggacaacgg gactggattg attttttgtc aacagagtcg
    17101 agtgtgacga gctctcttgt atttatatgt gaagtatatc acaaaagaaa gccttttcca
    17161 aggttttgga gacaaaaagt agaacaatcg attaactggt tgatgagact gtgaaaagag
    17221 cacaaagacc agcttgcaag ctggtcatac tcttcgaaaa tctcttcaaa ccacgtcagc
    17281 ttcatctaca acctcaaagc agtgctttga gcaacctacg gctagcttcc tagtttgctc
    17341 tttgattctc attgagtatc agtgtaggct ataagaataa gtcttgtagg gttttggcaa
    17401 ccccatcttg gtcgttggta agggaaattt gctcatctgc ataagggagt agctctggat
    17461 tggcattttt catggcataa cctttcccag caaaagcgag catttcggta tcattgtgtt
    17521 catctccaaa ggcaatcaaa tcttttttgt cacgattcat tatcttgagc aagtagtcca
    17581 aagcaaaggc cttgttgaca ccttttgggg tacattcaag gatattgagc ggacctcccc
    17641 aggtattgat agaaagttga tgctggtaga aggcgttcat gtcttttgcc aaggcatatt
    17701 tgtcactggc tctggtctgc aagaggatac agttagggtc cttggtcacc aattcaggct
    17761 ggaattgatc ttcaggctgg aaagcttcta caccaaatag tttgggattg gcaatctctt
    17821 cattgggatt tgtaatgtag aattttttac gatattctcc agcgataaaa tcggcttgaa
    17881 tgtcctctga acgttgaacc atatctagca gatatttttt gtctacagtc aaacactttt
    17941 caaaatccca aacttggtct ggtaaatgag taagggatcc gttgaagtta atcataggag
    18001 tgtctaagcc cagttcacgg taaaaatctt ttgacatacg gtaagggcga cctgtcgtaa
    18061 taataacctg atggcctttt tcagcaactt ttttgatggt ttttttggta aagtcagaaa
    18121 tttgactgtc tgagttgagc agggttccat ccaggtcaac tgcaataatt tttttcgtca
    18181 tatggacctt tctagtgtgt tttcagataa gatgtcttct attataacag aaagcaggca
    18241 gaaaagcaga ttccaaacaa aaaatgaagt tggattaatt tcttaaacaa atcaaacaaa
    18301 gacattgaaa aagtgaatga taaatgatat aattctatta ttgttcgtaa aaattaaaag
    18361 gagattgatg atggacaaat tatttaaact aaaagagaac ggtacagacg ttcgtacaga
    18421 ggttctcgct ggtttaacaa ctttctttgc aatgagctat attctctttg taaacccaca
    18481 aatactttca caaacaggaa tgcctgctca gggcgtcttc ctagcgacga ttattggtgc
    18541 agtagcgggt accttgatga tggcttttta tgctaactta ccttatgccc aagcgccagg
    18601 tatgggactc aatgccttct ttacctttac agttgtattc gggcttggtt attcttggca
    18661 agaagcccta gctatggtct tcatctgtgg gattatttca ttgattatta ccttgacaaa
    18721 tgttcgtaaa atgatcattg aatcgattcc caatgctctt cgctcagcta tttcagctgg
    18781 tatcggtgtc ttccttgcct atgtagggat taagaatgct ggacttttga aattcacgat
    18841 tgatccaggc aactatactg ttgtaggaga aggggctgac aaagctcaag caacgattgc
    18901 agcaaactct tcagcagttc caggattggt cagctttaat aatccagctg ttttagtggc
    18961 tcttgcagga cttgccatta ctatcttctt tgtcatcaaa gggattaaag ggggaattat
    19021 tctctctatc ttgacaacaa ctgttcttgc tattgcagtt ggtttggttg atttgtctag
    19081 tatcgatttt gctaataacc atgttggtgc agcttttgaa gatttgaaga caatctttgg
    19141 tgcagctctt ggttcagaag gtttgggagc tttggtttca gatacagctc gcttgcctga
    19201 aactctgatg gccattcttg ccttctcatt gacagatatt tttgacacaa ttggtacctt
    19261 gatcggtaca ggtgaaaaag ttggtattgt agcgacaaat ggtgaaaatc accaatcagc
    19321 caaattggat aaggctcttt actctgattt gattggaacg acagtcggtg ccattgcagg
    19381 tacttcaaac gtaacgactt atgttgagtc tgctgctggt atcggtgcag gtggacgtac
    19441 tggtttgaca gccttggttg tagctatctg ttttgcgatt tcaagcttct ttagcccact
    19501 tctagcgatc gtaccaacag cggctacagc tccaatcttg attatcgttg ggattatgat
    19561 gcttggtagc ttgaaaaata tctattggga tgatatgtct gaagcagttc ctgccttctt
    19621 cacatctatc tttatgggat tcagctactc tatcactcaa gggattgcag ttggtttctt
    19681 gacttacact ttgactaagc ttgtcaaagg tcaagttaaa gatgttcatg ccatgatttg
    19741 gattttggat gccttgttta tccttaacta catcagcatg gccttataat agaatgaccc
    19801 agggggattt tccccctttt ttaatacaaa ggagataggt gatgaaagag aaaaatatgt
    19861 ggaaagaatt gttgaatcgt gcaggctgga ttttggtctt tttacttgcc gtccttttat
    19921 atcaggttcc cctagtggtt acctctattt tgactttaaa agaagtagcc ctgctacagt
    19981 cagggctgat agttactggt atttctattg tggttctggt gctatttatt attggagctc
    20041 gtaaaacgca attggcaagt tttaattttt ctttttttag aactaaggat ttggcacgtt
    20101 tgggcttgag ttatttagtc attatctgtt caaatatact tggttctatc ttgttgcaac
    20161 tgtcaaatga gacgacaaca gctaaccagt ctcagattaa cgatatggtt cagaatagtt
    20221 ctctgatttc tagcttcttc ttgctagcct tgcttgctcc aatttgtgag gaaatcttgt
    20281 gccgtgggct tgttcctaaa aagattttcc gaggcaagga gaacttggga tttgtagtcg
    20341 gtacgattgt gtttgcttta ttgcatcaac caagtaattt accttcttta ttgatttatg
    20401 gaggtatgtc gacagttcta tcttggacgg cctacaagac ccaacgtttg gaaatgtcga
    20461 tcttgcttca catgattgtt aatgggattg ctttctgttt gttggctctt gtggtgatta
    20521 tgagtcggac attaggaatt tctgtttaaa agtttttatg taggaaccga cctctttcta
    20581 ctagggaaag atgaatgcaa tcgtgtccat ctttttcttt ttatggtaaa atagaaaaat
    20641 aatatgatga aaatccttga gggagtgacc gatatgtcaa gtaaagccaa tcatgcaaag
    20701 acagttattt gcggaattat caatgtaacc ccagactcct tttcggacgg tggtcaattt
    20761 tttgctcttg agcaggcact ccagcaggct cgtaaattga tagcagaagg agccagtatg
    20821 ctcgatatcg gcggagaatc gactcggccg ggaagtagct atgttgagat agaagaggaa
    20881 atccagcgtg ttgttccagt gatcaaagcg attcgcaagg aaagtgatgt cctcatctct
    20941 attgatactt ggaagagtca agtagcagag gctgctttgg ctgctggtgc cgatctagtc
    21001 aatgatatca ctggtctcat gggtgatgaa aaaatggccc atgtggttgc taaagctgga
    21061 gcgaaagtgg tcatcatgtt taacccagtt atggctcgac ctcagcatcc tagttcgctt
    21121 atcttccctc attttggctt tggtcaaacc tttacagaaa aagagttagc tgactttgaa
    21181 acattgccaa tcgaagactt gatggtggct ttctttgaac gagcactagc gagagcggca
    21241 gaagctggta ttgcaccaga aaatatcctg ttggatccag gaattggctt tggtctgacc
    21301 aagaaagaaa atcttctcct tttacgggac ctggataaac tacatcagaa gggctatcca
    21361 atctttctcg gagtgtcgcg caagcgattt gtcatcaata tccttgaaga aagtgggttt
    21421 gaagtcaatc ctgagacaga gcttggtttc cgaaatcggg acacggcttc ggctcatgta
    21481 actagtatcg ctgcgagaca gggtgtagaa gtggtgcgcg tgcatgacgt agctagtcac
    21541 aggatggcag ttgaaattgc ctctgccatt cgtctggctg atgaagcgga aaatttagat
    21601 ttaaaacaat ataaataaga tgaaagaaat tgaaaacaat cagtggattg ctaactaccg
    21661 gacggatcaa ccgcattttg gcttggaacg aatggtggaa ctgttagctt tgcgtggcaa
    21721 tccccatctt aaactcaagg ttctccatat cggagggact aacggcaagg gctcgactat
    21781 tgcttttttg aaaaagatgc tagaaaagct agggctgaga gttggcgtgt ttagttcgcc
    21841 ctatctcatt cattacacag accagattag catcaatggg gaatcgatct cagaagcgag
    21901 gctagaagct ctcatggcag actatcagtc tttgctggag ggagaagcgg tcgccaattt
    21961 acagggcaca accgagtttg agattatcac agccctagcc tatgactact ttgcctcaga
    22021 gcaagtagat gtggctatca tggaagttgg catgggtgga cttttggata gtaccaatgt
    22081 ctgtcagccc attttgacag gaattacaac tattggcttg gatcatgtgg ccctacttgg
    22141 tgacaccttg gaggccatag cagagcagaa ggcaggtatt atcaaacaag ggatgccctt
    22201 ggtaacaggg cgtattgctc cagaagcctt gactgtgatt gaccgcattg cggaagggaa
    22261 agatgcgccg agacttgcct acgggacaga ttatcaggtt cgtcatcaag aaagtgtggt
    22321 gacaggggaa gtctttgact atacaagtgc tgtcagacaa ggtcgcttcc agactagcct
    22381 gcttggtttg taccaaatag agaatgctgg gatggccata gctttacttg atactttttg
    22441 tcaagaagat ggtcgagagc tagcaagcaa tgactttctt ggtcaagcct tggaagaaac
    22501 aagttggcca gggcgtttgg aaatcgtgtc aagagatccc ttgatgattt tggatggagc
    22561 ccataatccc catgctatca aggccttgtt ggtaaccttg caagaacgtt ttgcggatta
    22621 tcataaggaa atcctcttca cttgtatcaa aaccaaagcc ttgggggata tgttggactt
    22681 gctgggagct atgccagata ccgagcttac tctaacacat tttgcggata gtcgggcgac
    22741 ggatgaaagc gtgctgaaag aggcagctaa gtctagaaat ctcagctacc aagattggca
    22801 tgattttcta gagcagaatt tgacagataa aaaagaagag aaacaaacag ttaggattgt
    22861 cacaggttcc ttgtatttct tgagccaagt gagggcctat ctgatggaga ggaagaacga
    22921 gaatggatac acaaaagatt gaagcagctg taaaaatgat tatcgaggct gtaggagaga
    22981 acgctaatcg cgagggcttg caggaaacac ctgctcgtgt agcccgtatg tatcaagaga
    23041 ttttttcagg tcttggtcaa acagcagagg aacatttgtc aaaatccttt gaaattattg
    23101 acgataatat ggtggtagaa aaggatatct ttttccatac catgtgtgaa caccacttct
    23161 tgccatttta tggtagagcg cacattgcct acattccaga tggtcgtgtg gcaggcttgt
    23221 ctaagctagc ccgtacggtt gaagtttatt cgaaaaaacc acaaattcaa gaacgtttga
    23281 atatcgaagt ggccgatgcc ttgatggact atctaggtgc taaaggagcc tttgttgtca
    23341 ttgaggcgga acatatgtgt atgagtatgc gtggtgttag aaaaccaggc actgcaacct
    23401 tgacgacagt agctcgtggt ctatttgaaa cagataagga cctccgcgac caagcttatc
    23461 gtttaatggg gctataaaaa gaatccgttt caagcggatt tttctagaaa ggaatcatta
    23521 tggatcaact gcagattaag gatttggaaa tgtttgccta tcatggtctt tttcctagtg
    23581 agaaagaatt ggggcagaaa tttgtcgttt cagccatcct atcctatgat atgaccaagg
    23641 cagctacaga cttggattta acagcctctg tccattacgg agaattgtgt cagcagtgga
    23701 cgacttggtt tcaggaaacg agtgaagatt tgattgaaac ggtagcctat aaactggtgg
    23761 aacgtacctt tgagtcttat cctcttgtcc aagaaatgaa gttggaactg aaaaaacctt
    23821 gggcaccggt gcatttgtca ctagatactt gctcggtaac cattcatcgc cgcaagcaac
    23881 gagcctttat cgccctagga agcaatatgg gagataaaca agcaaacttg aagcaagcca
    23941 ttgacaaact gcgagctcgt ggcatctata ttctcaaaga gtccagtgtc ttagcgacgg
    24001 agccttgggg tggagtggag caggatagct ttgccaatca agtggttgag gtggaaacct
    24061 ggctaccagc acaagacttg ttagaaacct tgttagccat tgagtcagag ctgggacggg
    24121 tgagagaagt gcattgggga cctcgtttga ttgatttgga cttgctcttt gtggaggacc
    24181 agatccttta tacagacgac ctcatattgc ctcatcctta catagcggaa cgcctttttg
    24241 tccttgagtc cttacaggaa attgcgcctc attttatcca tccgatatta aaacaaccga
    24301 tccgtaactt gtatgatgct ttgaaaaaat agaaaaactc tagttttcag ttacttgcaa
    24361 ctgaaggcta gagtttttaa aataggtcat tttcttctgg gaggaggata gtttctctac
    24421 cgtccatgtc taaaaccagt actcttgggg gataacgagg gtcgaaagga tggttaaagt
    24481 caaaatcaat ggctgtaggg aggtgttgac ttgaaaagtg gaaggtaatc tttccttggt
    24541 tattaagcaa ttgaaactcg agttcttctt ccaattcaaa gacatttttt aagaaatggt
    24601 cgataatata ccaaaaagag tcaatgatgt catcaggcaa gctggtaaca atgccaaaac
    24661 tagcagaccg catgtgggta ttggtaaaag ccatatctct gtctcctttc ttttccctta
    24721 tcatacagca aataggatta aaaatcaaga aaaggtgatt ttttgaaaag gattttaact
    24781 acagggaaaa agaaggaaaa aaattcccaa aaatctaccg aagttaatag gtaaattccc
    24841 aaattaactt gattatataa ctttcagtta ct
//
LOCUS       DAVMMR010000030        23980 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMMR010000030 DAVMMR010000000
VERSION     DAVMMR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.030"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..661
                     /locus_tag="VYS74_001179"
     CDS             <1..661
                     /locus_tag="VYS74_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3789227.1"
                     /translation="FYLNKGKVYGFVGPNGAGKTTIIKMILGILKPDSGKITIFNQTV
                     EQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANMKGLSLDTERLNEYLSMVGLKDVK
                     KKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPINGLDPQGVIEIRSLIRFLQEKKGI
                     TFLISSHILSELDKVISDIIIINYGKVEFFGSCHYLLQKYNCRNLEEAYLACLAGGEY
                     D"
     gene            654..1391
                     /locus_tag="VYS74_001180"
     CDS             654..1391
                     /locus_tag="VYS74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789228.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNVLLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1404..2114
                     /locus_tag="VYS74_001181"
     CDS             1404..2114
                     /locus_tag="VYS74_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789229.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISALYSE
                     LDYNKYWYILHTLIYMLMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFFLLPKLIKLKEQNLV"
     gene            2656..2877
                     /locus_tag="VYS74_001182"
     CDS             2656..2877
                     /locus_tag="VYS74_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789230.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3394..3647
                     /locus_tag="VYS74_001183"
                     /pseudo
     CDS             3394..3647
                     /locus_tag="VYS74_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3775..5004
                     /locus_tag="VYS74_001184"
     CDS             3775..5004
                     /locus_tag="VYS74_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3789231.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5024..7720
                     /gene="ppc"
                     /locus_tag="VYS74_001185"
     CDS             5024..7720
                     /gene="ppc"
                     /locus_tag="VYS74_001185"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV3789232.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7803..8020
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8133..9137
                     /locus_tag="VYS74_001186"
     CDS             8133..9137
                     /locus_tag="VYS74_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3789233.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9206..10072
                     /locus_tag="VYS74_001187"
     CDS             9206..10072
                     /locus_tag="VYS74_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789234.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10069..10827
                     /locus_tag="VYS74_001188"
     CDS             10069..10827
                     /locus_tag="VYS74_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789235.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            10917..12677
                     /gene="dnaG"
                     /locus_tag="VYS74_001189"
     CDS             10917..12677
                     /gene="dnaG"
                     /locus_tag="VYS74_001189"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV3789236.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12680..13789
                     /gene="rpoD"
                     /locus_tag="VYS74_001190"
     CDS             12680..13789
                     /gene="rpoD"
                     /locus_tag="VYS74_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV3789237.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13803..14132
                     /locus_tag="VYS74_001191"
     CDS             13803..14132
                     /locus_tag="VYS74_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3789238.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14166..15290
                     /gene="cpoA"
                     /locus_tag="VYS74_001192"
     CDS             14166..15290
                     /gene="cpoA"
                     /locus_tag="VYS74_001192"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV3789239.1"
                     /translation="MRKFPLFSSSLSFLLLILFKENDIIVVMEKKKLRINMLSSSEKV
                     AGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGR
                     KIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPR
                     EKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLA
                     EELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLF
                     LLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQAN
                     PAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK"
     gene            15306..16631
                     /locus_tag="VYS74_001193"
     CDS             15306..16631
                     /locus_tag="VYS74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV3789240.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16662..16838
                     /locus_tag="VYS74_001194"
     CDS             16662..16838
                     /locus_tag="VYS74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3789241.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            16897..17031
                     /locus_tag="VYS74_001195"
     CDS             16897..17031
                     /locus_tag="VYS74_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV3789242.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17095..18405
                     /gene="obgE"
                     /locus_tag="VYS74_001196"
     CDS             17095..18405
                     /gene="obgE"
                     /locus_tag="VYS74_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV3789243.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGGDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18415..18573
                     /locus_tag="VYS74_001197"
     CDS             18415..18573
                     /locus_tag="VYS74_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789244.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            19660..20919
                     /locus_tag="VYS74_001198"
     CDS             19660..20919
                     /locus_tag="VYS74_001198"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3789245.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            20935..21486
                     /locus_tag="VYS74_001199"
     CDS             20935..21486
                     /locus_tag="VYS74_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789246.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            21479..22756
                     /locus_tag="VYS74_001200"
     CDS             21479..22756
                     /locus_tag="VYS74_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3789247.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            22772..23632
                     /locus_tag="VYS74_001201"
     CDS             22772..23632
                     /locus_tag="VYS74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV3789248.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     FTSQGEEGTY"
BASE COUNT         7438 a         3982 c         5189 g         7371 t
ORIGIN      
        1 attttattta aataagggaa aagtatatgg ttttgttgga ccaaatgggg ctggaaaaac
       61 tacaattatc aaaatgattc tagggatttt aaaaccagat tctgggaaaa taactatttt
      121 taatcaaacc gtagaacaga attccgaaaa tatattatct aggataggat tagttttggg
      181 accttcattt tatggtcatc ttgatgctta taaaaatttg aagttaatag caaatatgaa
      241 agggcttagt ttagatactg aaagacttaa tgaatatcta tcaatggtag gattaaaaga
      301 tgttaaaaag aaaaaagtga aaaatttttc aatgggcatg aaacaacgtc taagtatagc
      361 tgcatcattg ttaggtagtc cagaaatatt aatttgggat gaaccaatca atggacttga
      421 tccacagggt gtaattgaaa ttagaagttt aatccgtttt ctacaggaga aaaaaggtat
      481 tacattttta atatcaagtc atattcttag tgaactggat aaagtgatat ctgatattat
      541 aattataaat tatgggaaag ttgaattttt tggttcttgc cactaccttt tacaaaagta
      601 taattgtcga aatctggaag aagcctactt agcttgttta gctggaggtg aatatgatta
      661 aattagagtt tctaaagcag aaaaaaagca tcttatggtt tgttcttatt tttccaataa
      721 ttctgaatgt tttgttatat atagacttaa cttttaggta tcgaggttat ttattggtgc
      781 atcaaaatga acttgcttta tctaattggc agttaatttt taaagaacaa acaatatttt
      841 atttttctga actattttat ttagttctat ctttaataat ttacgaagtg tttgcagtag
      901 aatttaaaaa tgatgcatgg ttaaccgtaa tttcattgcc atttagaaac aagtacacaa
      961 taaattctaa attactcatt acagtggttt acacatttac tttttggttg tctgattata
     1021 ttagcttata cgttattggg aaggctattg ataacagttt agagattggt ttgattttct
     1081 ttttaaaaac atttaccatt caattaattt cctcgttgat gataatgtta ttatattttc
     1141 taactttagt acttataagg aaaatttctg gaataatacc aattggtatt ataatgatga
     1201 ttttaacaat tagcatatac tacaacgatt ataattttaa gatttactta ccatttactt
     1261 atctttctca tgcttttaga gtgactgaaa gtcaatttta tatgatttta ctatctaata
     1321 taataatcat cgtacttttt tatatattaa ttcgtaaatt gaacgaaaga agttttgaga
     1381 tgaaacttta ataggtaata aaaatgaagt tacttaaaaa tgaattgatt aagagtaaaa
     1441 tatttttatt tattattgtt gacatctgta ttcagattct agtcattcta gctataaaaa
     1501 catatatttt agatatttcg gcactatact cagagcttga ttataataag tattggtata
     1561 ttttacacac tttaatatat atgctaatga tttttcctat acaaattttg tatcaaaatc
     1621 taagagaggc attgattgaa gataataata atggttggaa tatcatggtg attaacacta
     1681 ataatctcgt gaaaatcatc tatataaaag taacgataaa tatcgtgaga tgttttattt
     1741 gttattttgt ttatacaatt ttttctttaa ttcaattagg tggtatggga actgatatgt
     1801 tattaaccaa tataatattt cccaatataa tgtcatttct tttattttta cctatagcta
     1861 tctttatgca aatttgctgt ataagatttg attccatttt agcaaaggcc ttacctaata
     1921 ttttgctgat acttattgtt ttaatcacgt ttcagagtga ttggaatata tttatacctg
     1981 caacttatta ttatacagaa attcagtcaa ctacaaactt aggtattaaa ttattagttt
     2041 gtatctggat tatgggattt gaatttttcc ttcttccaaa actaataaaa ttgaaagaac
     2101 aaaatctagt ttaaatgttg ctcgaaaatt aaataattga atacgattaa caatgaaaca
     2161 atacaacgtg agtcatgact tcaaagtaat tgattttatg attctggttt cttacgctta
     2221 attaagcaag ttgtattcac tttatcaaat tgttattact ctaaaaagcg tctaacagct
     2281 atagagtgtc tgtttgcttc tctttacttt tattttaaat aattcattaa ccttccgtaa
     2341 gtgcccaata ataaggtgtg ttgaagaagt ctgagataaa atagttctca gtcacaaaaa
     2401 agctatagat tcccattggt gaacagctcc ctgtcaagta gacagtaaaa taatataaaa
     2461 tcttaagcga cctgattcct gaattccagt ggagtcaggt tgttttttaa ccgtctttct
     2521 taatattgtc ttacctataa tagatatgct tatgagatat gtttaataaa ttaattttag
     2581 ttcaaaatgt ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc
     2641 tcaggagaaa gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc
     2701 caaaaacaaa tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg
     2761 ggtgagcata cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt
     2821 acagactacc tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct
     2881 cctcaatttc atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat
     2941 agtccaaaat ggcactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga
     3001 gcgattttaa aaccatcaat aaaatagagc gatactttat atgccatgat acaaatgata
     3061 tacaatgata cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt
     3121 atgatgactt catcagtcat gccacgttaa atgtgtgagt ttgttagaga aacgcaatta
     3181 atcctcaaaa ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa
     3241 gcttgttgct aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac
     3301 aaaaatacaa tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg
     3361 tttcaagagc cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat
     3421 caacgcaacg taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc
     3481 tcatggtaac tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt
     3541 ttggcattca cgaaagcaac ttaatccgtc ggagccaatg ggttgaagta actcttgttc
     3601 aaaatggttt tacgatttca aattctgccc taattatgaa aaacagtaaa attcgaagga
     3661 ttgtaaggta agagtttttt tctttctgaa aaaatggtat aatagcaatc aaaactagaa
     3721 aataaaacgg aatttggaac agatttgtct gtatcctagt agagtggtga tgctatgaag
     3781 attagtaaga ggcacttatt aaattattcc atcttgattc cctacttgct tttatctatt
     3841 ttgggcttga ttgtggtcta ttcgaccacc agtgctattt taattgaaga aggcaagagc
     3901 gccttgcagt tggttcgaaa ccaaggaatc ttttggattg ttagtttgat actgattgcc
     3961 ttaatttata aattgagact agattttttg agaaatgagc gactaatcat tttagttata
     4021 ttaatagaaa tgcttttatt gttcttggct cgttttattg gtatttccgt aaacggggca
     4081 tacggttgga tttcggttgc aggagtaact attcagccag ctgagtactt aaaaatcatt
     4141 attatttggt atttagctca ccgattctcc aaacagcaag aagaaatagc tacttatgat
     4201 tttcaagttt tgactcaaaa tcaatggctt ccccgtgctt ttaatgattg gcgattcgtt
     4261 ctcctagttc tgattggaag tttgggaatt ttccctgatt taggaaatgc gactatttta
     4321 gtcttggttt ccttgattat gtatacagtt agtggaatcg cttatcgctg gttttcaacc
     4381 attctggcgc tcgtatctgc cgcttctgtc tttgtcttga ccactatcag cctaatcggt
     4441 gttgagacct tttcaaaaat tccagtattt ggctatgtag ccaagcgctt tagtgccttt
     4501 tttaatcctt ttgccgatcg tgctgatgca ggtcaccagt tagctaattc ttattttgcc
     4561 atggtcaatg gtggttggtt tggtctaggt cttggaaact cgattgaaaa acgaggttat
     4621 ttgccagaag ctcatacaga ctttgtcttt tctatcgtga ttgaagaatt tggctttgct
     4681 ggtgccagtc ttattttagc tctcttgttt ttcatgattt tgcggattat cttggtcggt
     4741 attcgagcgg agaatccttt caatgccatg gttgcactcg gtgtcggagg gatgatgttg
     4801 gttcaggtat ttgtcaatat cggagggatt tcgggcttga ttccatctac aggagtaacc
     4861 ttccccttct tatcccaggg tggaaatagt cttctagtct tatcagtggc agtagccttt
     4921 gtcttaaata ttgatgccag tgaaaaacgc gctaagttgt accgagaatt ggaaaatcaa
     4981 ccaatgaacc ttctgttgaa gtaggataaa gaaaggatag tttatgtctc ttcaaaaatt
     5041 agaaaattat agtaataaaa gtgttgtgca agaagaagtc ttgattctaa cagaattact
     5101 ggaagatatt actaaaaata tgcttgcccc agagaccttt gaaaaaataa tacagttgaa
     5161 agaattatca acgcaggaag attatcaagg tctaaaccgt ctagtgacta gcttatcaaa
     5221 tgatgaaatg gtctatattt cacgctattt ctctatctta cctcttttga tcaatatttc
     5281 agaggatgtg gatttagctt atgaaatcaa tcatcaaaat aatattgatc aggactattt
     5341 aggtaaatta tctacaacga ttaaattggt agcagaaaag gaaaatgccg ttgagatcct
     5401 agaacacttg aatgttgtcc ctgttttgac agcccatcca acacaagtgc aacgcaaaag
     5461 tatgttggat ttaacaaatc atattcatag tcttttgcgt aaataccgtg atgttaagtt
     5521 ggggttgatc aataaagata aatggtacaa tgatttgcgt cgttacatcg aaattatcat
     5581 gcagacagac atgattcgtg agaaaaaatt aaaagtgact aacgaaatca cgaatgctat
     5641 ggaatattat aacagctcct ttttgaaagc tgtacctcat ttgacgacgg agtataagcg
     5701 cttagcgcaa gcgcatggtc tgaatttaaa acaggctaaa ccaatcacca tgggtatgtg
     5761 gataggtggt gaccgtgatg gaaatccatt tgttacagca gagaccttga agcagtctgc
     5821 actcactcaa tgtgaagtca tcatgaacta ctatgataaa aagatttacc aactttatcg
     5881 tgaattttct ctttcaacta gcattgtcaa cgtcagcaag caagtcagag aaatggctcg
     5941 tcaatccaag gataactcga tttaccgcga aaaagagctt taccgtcgtg ccttgtttga
     6001 tattcaatca aaaattcagg caactaaaac ctatctgatt gaggatgaag aagttgggac
     6061 tcgttatgaa accgccaatg atttctacaa ggatttgatt gccattcgag attctctact
     6121 agaaaataag ggtgagtcct tgatttcagg tgattttgtg gaattattgc aggcagtaga
     6181 gatatttggt ttttacctag catcaattga tatgcgacaa gactctagcg tctatgaagc
     6241 ctgtgtggca gaactcttga aatcagcagg aattcattct cgttatagcg agttgagcga
     6301 agaagaaaag tgtgaccttc tcttgaaaga attagaagaa gatccccgaa ttctttctgc
     6361 gactcacgca gaaaaatcag aattattagc aaaagaatta gctattttta agacggctcg
     6421 tgttttgaaa gataagttgg gagatgatgt catccgtcag accatcattt cacatgcaac
     6481 cagcctttct gatatgctag aattagctat tctgttaaaa gaagtaggac tggtggatac
     6541 ggaaagggcg cgtgttcaga ttgttcccct ttttgaaaca attgaagact tggatcattc
     6601 agaagaaaca atgagaaaat atctttctct tagccttgcc aaaaaatgga ttgactcacg
     6661 aaataactac caagaaatca tgcttggcta ctctgacagt aataaagatg gcggttactt
     6721 gtcatcatgt tggaccctct acaaggctca acaacaattg actgctattg gagatgaatt
     6781 tggcgttaag gttaccttct tccatggtcg tggtggtact gtcggtcgtg gtggtgggcc
     6841 aacctatgaa gccattacat ctcaaccgct caagtctatc aaggatcgta tccgcttgac
     6901 ggagcagggt gaagtaattg ggaataaata cggtaacaaa gacgccgctt actataacct
     6961 tgaaatgcta gtatcggcag ctattaaccg tatgattact cagaagaaga gcgataccaa
     7021 taccccaaat cgttatgaag ccattatgga tcaagtagtg gaccgtagtt acgatatcta
     7081 ccgtgatttg gtctttggta atgagcattt ctatgattat ttcttcgagt caagtccaat
     7141 caaggctatt tcaagtttta atattggttc tcgtccagcc gctcgtaaga ctattactga
     7201 aatcggtggt ttgcgtgcca tcccttgggt attctcatgg tcacagagtc gtgttatgtt
     7261 ccctggatgg tacggggttg gttcaagctt caaggaattt atcaataaaa atccagagaa
     7321 tattgctatc ttacgagata tgtaccaaaa ttggcctttc ttccaatcgc ttctttcaaa
     7381 tgttgatatg gttttgtcaa aatcaaatat gaatattgct tttgaatatg ctaaactttg
     7441 tgaagacgag caagttaagg ccatctatga gactatttta aatgaatggc aagttactaa
     7501 gaacgttatc ttggctattg aaggacatga cgaactctta gctgacaatc catatctaaa
     7561 agctagtctg gattaccgta tgccttactt taatattctc aactatattc agttagagtt
     7621 gattaaacgc caacgtcgtg gagaattgtc cagtgatcaa gaacgattga ttcatatcac
     7681 catcaacgga attgcgacag gattgcgtaa ttcaggttga taattttcaa gagtgaatgc
     7741 taaaagtgaa tatcaaaaaa attctaatag actattgaca agtagtttaa aaatgatata
     7801 atttaaccat tcagaaaagt aatcatacaa actttttaga gagtctgtgg tagctgaaaa
     7861 cagataagtg gcgatgatga aaattgggct gaatgctatt tagaatttga aattataaaa
     7921 attcggtaag cacaccttac agtgcatctc gttattgcga gactgagcga tagggaaatt
     7981 ccctataatt gaggtggtac cgcgcatcga cgtcctcaca caagtttttt gtgtgaggat
     8041 ttttttgatg gaggttagta tggaaagaaa acgatggcgt cgcttgttta gataagtgaa
     8101 atatgttaaa ggaaataaaa aggagaaaca gaatgaaaaa taaacgttta attggaatta
     8161 ttgctgcatt agcagtctta gtagcaggaa gcttgattta ttcttcaatg aataaatcag
     8221 aagctcagaa taataaggat gagaagaaaa taactaagat tggtgtgctt caatttgtga
     8281 gccatccatc ccttgatttg atttataaag ggatccaaga tggacttgca gaagaaggat
     8341 ataaagatga tcaagttaaa attgatttta tgaactcaga aggtgaccaa agtaaggttg
     8401 cgacaatgag taaacaattg gttgcaaatg ggaatgacct tgtggttggt atcgcaacac
     8461 cagcagccca agggttggct agtgcaacaa aagacctacc ggttatcatg gccgctatta
     8521 cagacccaat tggtgctaac ttggttaaag atttgaaaaa accaggtggc aacattacag
     8581 gggtatctga ccacaatcca gctcaacaac aagttgaact catcaaggct ctgacaccga
     8641 atgtgaaaac aatcggagct ctttactcaa gtagcgaaga caattcaaaa acacaggtcg
     8701 aagaatttaa ggcttatgct gaaaaagcag gtctgacagt ggaaacattt gcagttcctt
     8761 caacaaatga aattgcctca actgtcactg ttatgactag caaggtagat gctatttggg
     8821 ttccaattga taacaccatt gcatcaggat ttccaacggt tgtctctagc aatcaaagtt
     8881 ctaagaaacc aatttatccc agtgcgacag ctatggtaga agtaggtggt ttggcatcag
     8941 ttgtaattga ccaacatgac cttggtgtgg caacaggtaa aatgattgtg caagtcttga
     9001 aaggtgcaaa accagccgat accccagtca atgtcttttc aactggtaag tcagtcatca
     9061 ataaaaaaat agcacaagaa ctaggtatta ctattcctga gtctgttctc aaagaagcag
     9121 gacaagtcat cgaataaata aggtagggag gaggagactc ttcccatttt ttaaaaagat
     9181 gaatatagaa aggattaaat aaaatatgat tgtttccatt atttctcaag gatttgtctg
     9241 ggctattcta ggtctgggaa tctttatgac atttaggatt ttaaactttc cagatatgac
     9301 gacagaaggt tccttccctc ttgggggagc tgttgctgtc actttgataa ccaaaggcgt
     9361 gaacccattt ttagcgacac ttgttgctgt aggagcaggt tgtttggctg gaatggcagc
     9421 aggccttctt tatacaaaag ggaagatccc aaccttgctc tcagggattt tggtgatgac
     9481 ttcttgtcac tcaatcatgc tcttgattat gggacgtgcg aatttaggcc tgcttggaac
     9541 caagcaaatt caggatgttt tgccttttga ttcggatttg aatcaactct tgacaggtct
     9601 catctttgtg agtattgtta ttgctctcat gctctttttc ttggacacta aactcggaca
     9661 agcctatatt gctacagggg ataatcctga tatggctaga agtttcggga ttcatactgg
     9721 acgcatggag ctcatgggct tggtcttatc aaatggtgtg attgcccttg caggtgccct
     9781 cattgctcag caagaaggtt atgccgatgt gtctcgaggg atcggggtta tcgttgtggg
     9841 gcttgcaagt ttgattattg gagaagttat tttcaagagt ttgagcttgg cagagcgttt
     9901 ggttactatc gttgtaggtt ctatcgctta tcaattttta gtgtgggcag ttatcgcact
     9961 tggctttaat acaagttacc ttcgtttata cagtgccttg attttagcag tctgcctcat
    10021 gattccaaca tttaagcaaa caatcttgaa aggagccaag ttaagcaaat gacagcaatt
    10081 gtagaattaa aaaatgcaac caaaatcgtt aaaaatggct ttgatgaaga aaagattatt
    10141 ttaaatgatg tttccttaga aatttttgaa cgggacttta tcacgatttt gggcggaaat
    10201 ggtgctggaa aatcaaccct ctttaacact atagcaggga ccttatcact aactagtgga
    10261 actatccgta ttttaggtga agaactcact aagttttcac ccgagaagcg tgccaagtac
    10321 ctgtctcgtg tcttccaaga tccaaagatg gggacagctc cccgtatgac ggtcgctgaa
    10381 aatcttttaa tcgccaagtt tcgtggtgaa aagcgtggat tgttaccacg acgcttgact
    10441 agctataagg atgaatttca ggcaaccatt gaaaaagtag gaaatggtct tgagaaacac
    10501 ttgaatacac cgattgagtt cttatcaggt ggacaaagac aggctttgag tctcttgatg
    10561 gcaaccttga agcgacctga attactcctg ttagatgagc atactgctgc cctggatcca
    10621 aagactagtg ttgctttgat ggaattgaca gatgaatttg ttaagaaaga tcagctaaca
    10681 gcccttatga ttactcatca tatggaagat gctctcaaat acggcaatcg cttgattgtc
    10741 atgaaagaag gacggattat ccaagattta aaccaagaag aaaaagcaaa aatgaaaatc
    10801 tctgattatt atcaactctt tgaataaagt agggaaaatg agtgaaatgg tttacttttt
    10861 ttctgaaata aagtatacta tataaagtaa actatgataa catggaggta ttgtgtatgg
    10921 ttgacaaaca agtcattgaa gaaatcaaaa acaatgccaa cattgtggaa gtcataggag
    10981 atgtgatttc tttacaaaag gcaggacgga actatctagg gctctgtcct tttcatggtg
    11041 aaaaaacacc ttctttcaac gttgtagagg ataagcagtt ttatcactgt ttcggttgtg
    11101 gtcgctcagg tgatgtcttt aagttcatcg aggagtacca aggggttccc tttatagagg
    11161 cagtccaaat cttaggtcag cgtgttggaa ttgaggttga aaaaccgctt tatagtgagc
    11221 agaagccagc ctcacctcac caagctcttt atgatatgca cgaagatgca gccaaatttt
    11281 accatgcgat tctcatgaca acgactatgg gcgaagaggc cagaaattac ctttatcagc
    11341 gggggttgac agatgaagtg cttaaacatt tttggattgg tttagcacct ccagaacgaa
    11401 actatctcta tcaacgtttg tctgatcagt atcgtgaaga ggatttactg gattcaggcc
    11461 tgttttatct ctcggatgcc aatcaatttg tagacacctt tcacaatcgc attatgtttc
    11521 ccctgacaaa tgaccaagga aaggtcattg ccttctcagg tcgtatctgg caaaaaacgg
    11581 attcacaaac ttctaagtat aaaaacagcc gatcgactgc aatttttaac aaaagttacg
    11641 aattatatca tatggatagg gcaaaaaaat cttctggaaa agctagtgag atttacttga
    11701 tggaaggatt catggatgtt attgcagcct atcgggctgg aatcgaaagt gctgtggcgt
    11761 cgatgggaac ggccttgagt cgagagcatg ttgagcatct gaaaaggtta accaagaaat
    11821 tggttcttgt ttacgatgga gataaggctg ggcaagccgc gacattgaaa gcattggatg
    11881 aaattggtga tatgcctgtg caaatcgtca gcatgcctga taacttggat cctgatgagt
    11941 atctacaaaa aaatggtcca gaagacttgg cctatctatt aacgaaaact cgtattagtc
    12001 cgattgagtt ctacattcat cagtacaaac ctgaaaacag tgaaaatctg caggctcaga
    12061 ttgagtttct tgaaaaaata gctcccttga ttgttcaaga aaagtccatc gctgctcaaa
    12121 acagctatat tcatatttta gctgacagtc tggcgtcctt tgattatacc cagattgagc
    12181 agattgttaa tgagagtcgt caggtgcaaa ggcagaatcg catggaagga atttccagac
    12241 cgacgccaat caccatgcca atcaccaagc agttatcggc tattatgagg gcagaagccc
    12301 atctactcta tcggatgatg gaatcccctc ttgttttgaa cgattaccgt ttgcgagaag
    12361 actttgcatt tgctacacct gaatttcagg tcttatatga cttgcttggc cagtatggaa
    12421 atcttcctcc agaagtttta gcagagcaga cagaggaagt tgaaagagct tggtaccaag
    12481 ttttagctca ggatttgcct gctgaaatat cgccgcagga acttagtgaa gtagagatga
    12541 ctcgaaacaa ggctctcttg aatcaggaca atatgagaat caaaaagaag gtgcaggaag
    12601 ctagccatgt aggagataca gatacagccc tagaagaatt ggaacgttta atttcccaaa
    12661 agagaagaat ggagtaataa tggcaacaaa acaaaaagaa gtaacaacat ttgacgtaca
    12721 ggtagcagaa tttatccgta atcataagca aaaagggaca gcaacagatg atgaaatcaa
    12781 tgcaagtctg gttattcctt ttaccttgga cgctgatggg attgaagatc tcttgcaacg
    12841 gattcaggat gcaggaattt ctatcacaga taacgaagga aatccaagtg cgcgtgttct
    12901 tagcaatgaa gaagaaccag aactcagcga tgaggacttg attgggtcaa cttctgctaa
    12961 ggtcaatgac cctgtccgta tgtacttgaa agaaataggg gtcgttcctc tcttgaccaa
    13021 tgaagaggag aaagagttgg cactggctgt tgaagctggt gatatcgaag ccaaacaacg
    13081 tcttgcggaa gccaatcttc gtttggttgt ttccattgcc aaacgctatg tcggtcgtgg
    13141 tatgcagttc cttgacttga ttcaagaagg aaatatgggc ttgatgaagg cggttgacaa
    13201 gtttgactat tctaaagggt tcaagttttc aacttatgca acttggtgga ttcgtcaggc
    13261 tatcactcgt gctattgcgg accaagctcg taccatccgt atcccagttc acatggttga
    13321 aactatcaat aaattggttc gtgaacagcg gaatctcctt caagaattgg ggcaagatcc
    13381 gacaccagaa cagattgctg aacgaatgga tatgacacct gataaggttc gtgaaatctt
    13441 gaagattgcc caagaaccag tatctcttga aactcctatc ggtgaagagg acgatagcca
    13501 ccttggagac tttatcgaag atgaagtgat tgaaaatcca gtggattata cgactcgtat
    13561 cgtcttgcgt gagcaattgg atgaaatctt agatactctt acagaccgtg aagaaaatgt
    13621 tctgcgtcta cgttttggac tagatgatgg aaaaatgcgc acacttgaag atgtggggaa
    13681 agtctttaac gtaactcgtg agcgtatccg tcagattgaa gcaaaggctt tgagaaaact
    13741 acgccaacca agtcgtagca aaccgcttcg tgattttatt gaagactaag agtgaggaaa
    13801 atatggctta tacagaagag caaattgaaa acatcaaaac acggatttta acagccttgg
    13861 aagaagtcat cgaccctgag ttgggaatcg atattgtcaa tcttggtttg atctatgaga
    13921 ttcgttttga cggtgacaca gggcaaacag agattgacat gactttgaca actatgggtt
    13981 gtcccttagc agaccttttg acagaccaga tttatgatgc tatgatagag gttccagaag
    14041 taacggatac tgaggttaaa ttagtctggt atccagcctg gacagttgaa aaaatgagtc
    14101 gctatgcccg cattgcccta ggcattaagt aaacatataa aagcatgtga gagactgttg
    14161 gaaaagtgag gaaatttccc ctcttttcct ctagtctctc ctttcttttg ctgattttat
    14221 tcaaagaaaa tgatataata gtagttatgg agaaaaagaa attacgcatc aatatgttga
    14281 gttcaagtga gaaagtagca ggacagggag tttcaggtgc ttaccgtgaa ttagttcgtc
    14341 ttcttcaccg tgctgccaag gaccaattga ttgttacaga aaatcttcca atcgaggcag
    14401 atgtgactca ctttcatacg attgattttc cctattattt atcaaccttc caaaagaaac
    14461 gctcagggag aaagattggc tatgtgcatt tcttgccagc tacacttgag ggaagtttga
    14521 aaattccatt tttcttaaag ggaattgtga aacgctatgt attttctttt tacaaccgga
    14581 tggagcactt ggttgtggtc aatcctatgt ttattgagga tttggtagca gctggtattc
    14641 cacgtgaaaa agtgacctat attcctaact ttgtcaacaa ggaaaaatgg catcctctac
    14701 cacaagaaga ggtagttaga ctgcgcacag atcttggtct tagtgacaat cagtttatcg
    14761 tagtaggtgc tgggcaagtt cagaaacgta aagggattga tgactttatc cgtctggctg
    14821 aggaattgcc tcagattacc tttatctggg ctggtggctt ctcttttggt ggtatgacag
    14881 atggttatga acactataag aaaattatgg aaaatccccc taaaaatttg atttttccag
    14941 gcattgtatc gccagagcgg atgcgcgaat tgtatgctct agcggatctt ttcttgttgc
    15001 ctagttacaa tgagctcttt cctatgacta ttttagaagc tgcgagttgt gaggctccta
    15061 ttatgttgcg tgatttagat ctctataagg tgattttgga gggaaattat cgggcgacag
    15121 cgggtagaga agagatgaaa gaggctattt tggaatatca agcaaatcct gctgtcttaa
    15181 aagatctcaa agaaaaggct aagaatattt ccagagagta ttctgaagag catctgttac
    15241 aaatctggtt ggacttttat gagaaacaag ccgctttagg gagaaagtaa aaagtgaggt
    15301 aatctatgcg aattggttta tttacagata cctattttcc tcaggtttct ggtgttgcga
    15361 ccagtattcg aaccttgaaa acagaacttg aaaagcaggg acatgctgtt tttatcttta
    15421 cgacgacaga taaggatgtc aatcgctacg aagattggca aattatccgc attccaagtg
    15481 ttcctttctt tgcttttaag gatcgtcgct ttgcctaccg aggttttagc aaggcacttg
    15541 aaattgctaa acagtatcag ctagatatta tccatactca gacagaattt tctcttggcc
    15601 tgttgggaat ttggattgcg cgtgaattga aaattccagt catccatacc tatcacaccc
    15661 agtatgaaga ctatgtccat tatattgcta aggggatgtt gatccggccg agtatggtca
    15721 agtatctggt tagaggtttc ctgcatgatg tggatggggt tatttgccct agtgagattg
    15781 tccgtgactt gctatctgat tataaggtca aggttgaaaa acgggtcatt cctactggga
    15841 ttgaattagc caagtttgag cgtccggaaa tcaagcagga aaatttgaaa gaactgcgta
    15901 gtaaactagg gattcaagat ggtgaaaaga cgttgcttag tctttcgaga atctcctatg
    15961 aaaaaaatat tcaagcagtt ttagcagcct ttgctgatgt tctgaaagag gaagacaagg
    16021 ttaaactggt agtagctggg gatggccctt atctgaatga cctcaaagag caagctcaga
    16081 acctagagat tcaagactca gtcatcttta cagggatgat tgctcctagt gagacggctc
    16141 tttactataa agcggcggat ttcttcattt cggcatcgac aagcgaaacg caaggtttga
    16201 cctacttgga aagcttagcc agtggaacac ctgtcattgc tcacggaaat ccttatttga
    16261 acaacctcat cagtgataaa atgtttggaa ccttgtacta tggagaacat gatttggctg
    16321 gtgctatttt ggaagccctg attgcaacac cagacatgaa cgagcatacc ttatcagaga
    16381 aattgtatga gatttcagct gagaactttg ggaaacgagt gcatgagttt tatctggatg
    16441 ccattatttc aaataacttc cagaaagatt tggctaaaga tgatacggtc agtcagcgta
    16501 tctttaagac agttttgtat cttcagcaac aggtggttgc tgtacctgta aaaggatcta
    16561 gacgcatgtt gaaggcttca aaaacacagt tgatcagtat gagagactat tggaaagacc
    16621 atgaagaata gaaagaggaa cagctatgaa aaaaacaatt aatgagaagc ggtcgtgata
    16681 aaaagattgc gggtgtttgt gctggggtgg cccattatct ggatatggat ccgactatcg
    16741 ttcaagtcat ttggggtgtt cttacttgct gttacggagc tggaattgta gcttacatta
    16801 ttttatggat tatcgcacca gtagcaactg actattgaaa actagctcaa ttcatgctaa
    16861 gataatagaa aatagaatgt aacgaaggag agaaaaatgg catttggaga taatggaaat
    16921 cgtaaaaaaa ctatgtttga gaaaataacc ttgtttatcg tgattatcat gctagtagca
    16981 agtttattgg gaatttttgc aactgcaatt ggtgccctca gtaatctata aaatagattc
    17041 aagaaaattt agtgactggg atttcccagc ccttttttaa agtgagaaga aataatgagt
    17101 atgtttttag atacagctaa gattaaggtc aaggctggta atggtggcga tggtatggtt
    17161 gcctttcgtc gtgaaaaata tgtccctaat ggaggccctt ggggtggtga tggtggtcgt
    17221 ggaggcaatg tggtcttcgt tgtagacgaa ggactacgta ccttgatgga tttccgctac
    17281 aatcgtcatt tcaaggctga ttctggtgaa aaagggatga ccaaagggat gcatggtcgt
    17341 ggtgctgagg accttagagt tcgagtacca caaggtacga ctgttcgtga tgcggagact
    17401 ggcaaggttt taacagattt gattgaacat gggcaagaat ttatcgttgc ccacggtggt
    17461 cgtggtggac gtggaaatat tcgtttcgcg acaccaaaaa atcctgcacc ggaaatctct
    17521 gaaaatggag aaccaggtca ggaacgtgag ttacaattgg aactaaaaat cttggcagat
    17581 gtcggtttag taggattccc atctgtaggg aagtcaacac ttttaagtgt tattacctca
    17641 gctaagccta aaattggtgc ctaccacttt accactattg taccaaattt aggtatggtt
    17701 cgcacccaat caggtgaatc ctttgcagta gccgacttgc caggtttgat tgaaggggct
    17761 agtcaaggtg ttggtttggg aactcagttc ctccgtcaca tcgagcgtac acgtgttatc
    17821 cttcacatca ttgatatgtc agctagcgaa ggccgtgatc catatgagga ttacctagct
    17881 atcaataaag agctggagtc ttacaatctt cgcctcatgg agcgtccaca gattattgta
    17941 gctaataaga tggacatgcc tgagagtcag gaaaatcttg aagaatttaa gaaaaaattg
    18001 tctgaaaatt atgatgaatt tgaagagtta ccagctatct tcccaatttc tggattgacc
    18061 aagcaaggtc tggcaacact tttagatgct acagctgaat tgttagacaa gacaccagaa
    18121 tttttgctct acgacgagtc cgatatggaa gaagaagctt actatggatt tgacgaagaa
    18181 gaaaaagcct ttgaaattag tcgtgatgac gatgcgacat gggtactttc tggtgaaaaa
    18241 ctcatgaaac tctttaatat gaccaacttt gatcgtgatg aatctgtcat gaaatttgcc
    18301 cgtcagcttc gtggtatggg gggtgatgaa gcccttcgtg cgcgtggagc taaagatggg
    18361 gatttggtcc gcattggtaa atttgagttt gaatttgtag actaggagac tggtatggga
    18421 gataaaccga tatctttccg agatgcggat ggtaattttg tttccgccgc agacgtttgg
    18481 aatgaaaaga aattggaaga actatttaat cgtctcaatc caaatcgtgc cttgagattg
    18541 gcacgaacta aaaaggaaaa tccatctcag taaagaagct aaaaaatccc gtgcctcatc
    18601 agacacggga ttttgtggta cgacaggcat gtatagcaaa ctgaatctgg aatagcacag
    18661 catatcttct aaaatattgc tataaattga tttgaatttc aaaattaaat tgttttattc
    18721 ttatttcaat ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagtgatt
    18781 cccaagcctg actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag
    18841 tctgatgctg gaaataagaa ttgtcagaag aagggatagc gaaatcgtgg ctctacgaac
    18901 aggaacgtga taataaggcg tatatagcga ataagagggc ctcaaactct aaagtccaaa
    18961 aaggtagtcg taacctatat gcgtaaatca tgagagtaat tgaattcgta ctaagatttt
    19021 ctatttttac tgtaaccttt taacgtcctc atatcttgta tacacgagga aagatgtacg
    19081 acttatcccg tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt
    19141 cacggttatc tttaaaatag attgtctgat aggatgctag cctgaaaacg tcttgttcat
    19201 gaaatatagg acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct
    19261 tgctaaaatt tagttgaaca gccgtattcc gatacttaga taagagatct agtcttagct
    19321 cctactcagt ttcaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt
    19381 cgaaaatctc ttcaaatcac gtcaatatcg ccttgccgta tgtgtaggat actaactacg
    19441 tcagttccat ctacaatctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
    19501 tgatctttga tttttattga gtattagtaa ttcagttact aactcgtcaa ctctgattta
    19561 tccaataaaa ttgaaaagga tggaaaaaag gataaattta tgatatactt tattttgaag
    19621 accttattag aaatcttgaa agagtattga aaacttagaa tgagaaaaat tgttatcaat
    19681 ggtggattac cactgcaagg tgaaattact attagtggtg ctaaaaatag tgttgtggcc
    19741 ttaattccag ctattatatt ggctgatgat gtggtgactt tggattgtgt tccagatatt
    19801 tcggatgtag ccagtcttgt cgaaatcatg gaattaatgg gagctactgt taagcgttat
    19861 gacgatgtct tggagattga tccaagaggt gttcaaaata ttccaatgcc ttatggtaaa
    19921 attaacagtc ttcgtgcatc ttactatttt tatgggagcc tcttaggccg ttttggtgaa
    19981 gcgacagttg gtctaccggg aggatgtgat cttggtcctc gtccgattga cttacacctt
    20041 aaggcgtttg aagctatggg tgccactgct agctacgagg gagataacat gaagttatct
    20101 gctaaagata caggacttca tggtgcaagt atttacatgg atacggttag tgtgggagca
    20161 acgattaata cgatgattgc tgcggttaaa gcaaatggtc gtactattat tgaaaatgca
    20221 gcccgtgaac ctgagattat tgatgtagct actctcttga ataatatggg tgcccatatc
    20281 cgtggggcag gaactaatat catcattatt gatggtgttg aaagattaca tgggacacgt
    20341 catcaggtga ttccagaccg cattgaagct ggaacatata tatctttagc tgctgcagtt
    20401 ggtaaaggaa ttcgtataaa taatgttctt tacgaacacc tggaagggtt tattgctaag
    20461 ttggaagaaa tgggagtgag aatgactgta tctgaagaca gcatttttgt cgaggaacag
    20521 tctaatttga aagcaatcaa tattaagaca gctccttacc caggctttgc aactgatttg
    20581 caacaaccgc ttacccctct tttactaaga gcgaatggtc gtggtacaat tgtcgatacg
    20641 atttacgaaa aacgtgtaaa tcatgttttt gaactagcaa agatggatgc ggatatttcg
    20701 acaacaaatg gtcatatttt gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa
    20761 gcgaccgact taagagctgg ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa
    20821 actgaaatta ccaatatcga gtttatctta cgtggttatt ctgatattat cgaaaaatta
    20881 cgtaatttag gagcggatat tagacttgtt gaggattaaa ccgtagaggt gtttatgaat
    20941 atttggacca aattagcaat gttttctttt tttgaaacgg atcgcttgta tttgcgtcct
    21001 ttctttttta gtgatagtca ggacttccgc gagatagctt caaatccaga aaatcttcaa
    21061 tttattttcc caacgcaggc aagtctggaa gaaagtcaat atgcactggc caattacttt
    21121 atgaagtccc ctttgggagt gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt
    21181 tctattaaat ttgagaagtt agatgaaatc aaaaaagaag ctgagcttgg ctattttttg
    21241 agaaaagatg cttggtcgca aggatttatg acagaggttg ttagaaaaat ttgtcagctt
    21301 tcttttgagg aatttggctt aaaacaatta tttatcatta cccaccttga aaatgaagct
    21361 agccaaagag ttgctcttaa gtctggattt agtttgttcc gtcagtttaa gggaagtgat
    21421 cgttacacaa gaaaaatgcg ggattatctt gaatttcggt atgtaaaagg agagttcaat
    21481 gagtaagcat caggaaattc taagctattt ggaggaatta ccagtaggta aaagggtcag
    21541 tgttcgtagc atttcgaatc atctaggagt tagtgatgga acagcctatc gagctattaa
    21601 agaagctgaa aaccgtggaa ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa
    21661 atcccagaaa gttgctatag agagattaac gtttgctgaa attgcagaag tgacttcttc
    21721 tgaggttctg gctgggcaag aaggtttaga gagagagttt agtaagtttt caattggtgc
    21781 catgactgaa caaaatatct tgtcttacct tcatgatggg gggctcttga ttgtcggaga
    21841 ccgaacccgt attcagttgc tagccttgga aaatgaaaat gcagttctgg ttacaggggg
    21901 atttcaggtt catgatgatg tgcttaaact ggccaatcaa aaagggattc ctgttctaag
    21961 aagtaagcat gataccttta ccgtcgcgac catgatcaat aaagccttgt caaatgtcca
    22021 aatcaagact gatattctga cagttgagaa actttatcgc cctagtcatg agtatggttt
    22081 tctgagagag acagatacag ttaaagatta tttggacttg gttcgtaaga atcgtagcag
    22141 ccgtttccct gttatcaatc aacatcaggt cgttgttggc gttgtaacca tgagagacgc
    22201 tggtgataaa tcaccaagca cgacaattga taaggttatg tctcgtagtc tatttttggt
    22261 tgggttatcg acaaatattg ccaatgtgag tcaacggatg atcgcagaag actttgaaat
    22321 ggtaccagtt gttcgaagca atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat
    22381 ggagaagatg agccgttccc aagtttcggc tctaccaact ttttctgagc agattggaca
    22441 aaagctctct tatcaccatg atgaagtagt cattacagtg gaacccttta tgctagaaaa
    22501 aaatggagtt ttggctaatg gtgtattggc agaaattctg acccacatga cccaagattt
    22561 agttgttaat agtggtcgca atctcattat cgagcagatg ctgatctact ttttgcaggc
    22621 tgttcagata gatgatatat tgcgcattca ggcacggatt attcatcata cgagacggtc
    22681 agctataatt gattacgata tttatcatgg tcaccagatt gtttcaaaag caaatgtgac
    22741 tgttaaaatt aattagaaac taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat
    22801 cgaagctatg gacaaggctg gtgattttct agcaagtatt catataggct tacgtgattt
    22861 gattaagcca ggcgtagata tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga
    22921 agaaaatttc cttccacttc agattggggt tgacggtgcc atgatggact atccttatgc
    22981 tacctgttgc tctcttaacg atgaagtggc tcacgctttc cctcgtcatt atatcttgaa
    23041 agatggtgat ttgctcaaag ttgatatggt tttgggaggt cccattgcta aatctgacct
    23101 gaatgtctca aaattaaact tcaacaatgt tgaacaaatg aaaaaataca ctcagagcta
    23161 ttctggtggt ttagcagact catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt
    23221 caaaaacttg atggatgtaa ccaaagaagc tatgtacaag ggtattgagc aagctgttgt
    23281 tggaaatcgt atcggtgata tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta
    23341 cggtgtagtg cgtgatttgg ttggtcatgg tgttggccca actatgcacg aagaaccaat
    23401 ggttcctaac tatggtattg caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac
    23461 cattgaacca atgatcaata caggcgattg ggaaattgat acagatatga aaactggttg
    23521 ggcgcataag accattgacg gtggattgtc atgtcagtat gaacaccaat ttgtcattac
    23581 gaaagatgga cctgttatct ttactagcca aggtgaagaa ggaacttatt aataaaaagt
    23641 gaaaagacta ctggaagttt attttgataa aaaatccagt agatcttttc ataataaaac
    23701 gcattgtatc aagtgttagg agctgatatc atgcgttttt ctgcttttaa gattttttcc
    23761 aactctgttt gtaagcgcat cataacaaag ggtctaggat tcagggctct cctcctatat
    23821 actattagta aagtaaaact aagggaggat attttagtgt cgcagtctat tgttcctgta
    23881 gagattccac aatattgtcg ttttgattct aaaaagagaa atggaattct gtttaacgtt
    23941 cgtattgcca atcttaaatt tacctttttt agattagact
//
LOCUS       DAVMMR010000031        23498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMMR010000031 DAVMMR010000000
VERSION     DAVMMR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.031"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(126..350)
                     /locus_tag="VYS74_001202"
     CDS             complement(126..350)
                     /locus_tag="VYS74_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEV3789249.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(355..534)
                     /gene="asp4"
                     /locus_tag="VYS74_001203"
     CDS             complement(355..534)
                     /gene="asp4"
                     /locus_tag="VYS74_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEV3789250.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(536..1873)
                     /gene="gtfB"
                     /locus_tag="VYS74_001204"
     CDS             complement(536..1873)
                     /gene="gtfB"
                     /locus_tag="VYS74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEV3789251.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1866..3377)
                     /gene="gtfA"
                     /locus_tag="VYS74_001205"
     CDS             complement(1866..3377)
                     /gene="gtfA"
                     /locus_tag="VYS74_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEV3789252.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3389..5761)
                     /gene="secA2"
                     /locus_tag="VYS74_001206"
     CDS             complement(3389..5761)
                     /gene="secA2"
                     /locus_tag="VYS74_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEV3789253.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5754..6194)
                     /gene="asp3"
                     /locus_tag="VYS74_001207"
     CDS             complement(5754..6194)
                     /gene="asp3"
                     /locus_tag="VYS74_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEV3789254.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6191..7726)
                     /gene="asp2"
                     /locus_tag="VYS74_001208"
     CDS             complement(6191..7726)
                     /gene="asp2"
                     /locus_tag="VYS74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV3789255.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7710..9290)
                     /gene="asp1"
                     /locus_tag="VYS74_001209"
     CDS             complement(7710..9290)
                     /gene="asp1"
                     /locus_tag="VYS74_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEV3789256.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9300..10517)
                     /gene="secY2"
                     /locus_tag="VYS74_001210"
     CDS             complement(9300..10517)
                     /gene="secY2"
                     /locus_tag="VYS74_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEV3789257.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(10574..11251)
                     /locus_tag="VYS74_001211"
     CDS             complement(10574..11251)
                     /locus_tag="VYS74_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3789258.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11236..11480)
                     /locus_tag="VYS74_001212"
                     /pseudo
     CDS             complement(11236..11480)
                     /locus_tag="VYS74_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(11480..12676)
                     /locus_tag="VYS74_001213"
     CDS             complement(11480..12676)
                     /locus_tag="VYS74_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV3789259.1"
                     /translation="MRKSIVLAADNVYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNSGVLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12685..13905)
                     /locus_tag="VYS74_001214"
     CDS             complement(12685..13905)
                     /locus_tag="VYS74_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV3789260.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13930..14814)
                     /locus_tag="VYS74_001215"
     CDS             complement(13930..14814)
                     /locus_tag="VYS74_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3789261.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYIHVFDSDYYVKNYNSKLLMEELPLLERYDM
                     SFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYKLFMQVEKVTYINKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRHLEMLDYLLNGNKYN"
     gene            complement(14811..17264)
                     /locus_tag="VYS74_001216"
     CDS             complement(14811..17264)
                     /locus_tag="VYS74_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEV3789262.1"
                     /translation="MIKTIVLAGNRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIASMLGSEIIDVKLPEQTVFQDWKKQAHISSIAYARYFIPDYIQEDTVLYLD
                     CDLLINDKLDSLFEQDVREHYIAAIRDANGQGFNTGVLLINNEKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWNQILQHHLGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLAYHLSRKGYQVLDIGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIQF
                     IEDETYNSQILVDLSKREV"
     gene            complement(17274..18284)
                     /locus_tag="VYS74_001217"
     CDS             complement(17274..18284)
                     /locus_tag="VYS74_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3789263.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKRTFFAGSLERFPDLQNWSQDTPLRVFSNKGEAGSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(18313..19188)
                     /locus_tag="VYS74_001218"
     CDS             complement(18313..19188)
                     /locus_tag="VYS74_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3789264.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVVAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(19193..20407)
                     /locus_tag="VYS74_001219"
     CDS             complement(19193..20407)
                     /locus_tag="VYS74_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV3789265.1"
                     /translation="MYRKVIALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSE
                     WFLLMRNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYL
                     DSDIIVNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKSTQN
                     LLELTAQHHEHVYGDQGVLNIYFGDQWLHLDKEYNFMVGLDQFLHLKGNADWYQSNYY
                     GNYEPKIVHYTTEFKPWTHLTLTRFRKLWWFYYGLNWNDVLLSSDIVKRSFKELVGVP
                     LYHTCIFTNSAAMESLEYLLSELPEVHFTILAHTNFAPSVVDLQRYLNLSLFPNFNSF
                     NMKETLDKMDFYLDINHEGEIVNIIEEVCRRNKPIFSFDNTSHDSKGRSRIFSATEPD
                     KMVDAIRKFLGE"
     gene            complement(20764..21642)
                     /locus_tag="VYS74_001220"
     CDS             complement(20764..21642)
                     /locus_tag="VYS74_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV3789266.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNNGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKMRV"
     gene            complement(21639..23039)
                     /locus_tag="VYS74_001221"
     CDS             complement(21639..23039)
                     /locus_tag="VYS74_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV3789267.1"
                     /translation="MHALVDAMSERITLLANMGYPLEKHLAIYRQMLEFSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIRSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            complement(23086..>23498)
                     /locus_tag="VYS74_001222"
     CDS             complement(23086..>23498)
                     /locus_tag="VYS74_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3789268.1"
                     /translation="TSASESASTSASASASTSASASASTSASESASTSASASASTSAS
                     ASASTSASASASTSASESASTSASASASTSASASASESASTSASASTSAGKSRQQLPN
                     TGTEASKSSVLLGALAAVTGLGLFAKRRKRDDEE"
BASE COUNT         7568 a         5011 c         3602 g         7317 t
ORIGIN      
        1 acaacctcaa aacggtgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt ataaaaaaga aaacttcaga ccaatccaca aaaggtgaat atctgaagtt
      121 ttcttttatt tataagtaat caccataaag gttaatagta gaacaaataa agccaaactc
      181 accaataaag tgagcacctt gaccaaggtg ttgctctgcc agtagcttgg tttaccaata
      241 ttactagtgg catccatgga aaatagttga ttttgacggg gttgtatagt taccaataca
      301 atcaaagcaa gagataggat aatagctaaa acaattagta tttcaatcat agatctacct
      361 caaaattccc agcaaagtaa agagcaaacc aatcagaatc atcaaaccca gtaaaagtga
      421 aaaagtctta ctaatctttt cccctcgggt ttctttttcc ttcttgatgg gtttttgttt
      481 caactcttcc atgcggcctt caacgtcttt gtaaaataaa tcttcctctg acatgttagc
      541 ctcctaaaac agtttgcatg gtttcctgat atctcaccaa gtcaacataa tttgcatgtt
      601 ggccttgttt gccaagtgcc tgacgcattt gatcaacatc tgaaagggcc aatttaatgg
      661 tctcaaccaa agcagcaact tcactacttt caaatagatg gtctggagcg atataaagtc
      721 tattgtgcac cgtctgatta aagccaagaa tcaagagatt gtgctcaaag gcctgacgca
      781 ctgcctgtag taactcatta ctgtggttta tatccaagta aatatccgac agttgataca
      841 gctcctgaat cttctgtgga ctagcattct ggtaaaggac cacattagga tagcgaagca
      901 tgtctaagag cttagaagac atctctgtca ccgctgcaat acggaaagtg acatcaggca
      961 aggctcctgc gattgcttct acttgctcaa tctgatctga attggtcaag attaaggcat
     1021 ctcgtcttag gaaattatca cgtttgaact ggtaatgata acccaagtgc acaaactgat
     1081 catggtattt ctcgtcagtt aactctaaag cgcgctcata agtcgcctta tttggaatga
     1141 tgatcttctt agtacgcaca ttatcacttt ccaaaatcaa ctgcatatta cctggaatgg
     1201 catcatagag aggttcctgc cataccaaga catccgagcc agatttatct ggatgatgga
     1261 aggaaaccaa gaaaggagtc gctagagtat taaagataag ctgactggta tctatttcca
     1321 aatcttgcaa aaagaaggtg ataaattcaa ctttatttgc aaagtaacgc atggactgac
     1381 caggcaaagt caataagata tcacccgtca catggttttc cagtaaaact tgttggccat
     1441 tgacatcttg gtaaactgtc ataatcggct cgctatctgc gctataagtg gttttagcaa
     1501 agcaagcacc aaagcgattg tagtggtcaa cctgacgtac tcgaccttct aggtctttcc
     1561 agtctacctg tttaaccaag cgagcctgca ttccatcaac ataatgaatg acagccctct
     1621 cctgcgtcac atcttcaata caagcagact gattatctcc taaaatttcc caaaaatctg
     1681 aaacgggaac ttggttaaaa tagagaggtt ttccatcctc gtaacctaga taataggtaa
     1741 aaggagaaag cagaccatca ggcagaaaac catctgcatc gatgaccact ccaagttgag
     1801 aaagaccagt agcgactaga ctttcatgta aatctcgact ttcatgacta taacgatcat
     1861 aaagttcaat catggagcac ctcctctact gttttcttcc acttttctaa aatttctttg
     1921 gtcaagaagc cttctgcaat ttggtaagaa taggcacgca tagcttccaa acgattttct
     1981 tgatacaatt gacaaatctt agcggcataa gcttgcttga tttggtcttc tacatggtca
     2041 gatgaacttg gaatcaaata accattttgc ccatcctcta taaaggtctg attaccataa
     2101 ggcacatcaa aaccaattag aggtagacct gaaccaatag cttccatcaa ggtcagacca
     2161 aatccttcgc tggtagaagc cgttaagtag acctcatact ggctataaat ctgcgaaagt
     2221 tccgcatgcc ccttgagttg gatatagtcc tctgcctgat gatttgcaat aatgtctcta
     2281 agcagagaat cttctccacc actaccatag atatcaaagg ttagttccgg taactcctta
     2341 tgagcttcaa tcacagcttt cacaagccaa tcaatgtgct tttctttggc aagacgtgaa
     2401 gccgtaatca atgaaaatgg tttgcgccct tgacttgaat ctgtcaagga atcaatactg
     2461 cctacaggaa tggtaacaat ctttggctga tgctgagtat atttggcaaa ttgctcttgt
     2521 agaacttcat tttgtctatc agtagacacg ataaagaagt caaccttatc tgcattggta
     2581 aactgatagt cataatagtt attccaaagg atgtagtcct catttgtagc attttcacta
     2641 taatgctccg catgaacaac taccgctaaa tgtgctgtct gtgcttcctc aaacacaacc
     2701 tgaccaatgc ctgtctccct atcgagaatg accaaatcag acttattcaa attcaaagat
     2761 ttcataaagg cacgcacaaa agcttgcttt ccatagaaaa tcttatcctt gaaatgataa
     2821 acttcttcct tcccttgatt catcaagata tcatagactg gagtcccgtc ttcattataa
     2881 aaagttcgtt ggtataagac tgcaacattg tccttgggag caaaatactc gctacaataa
     2941 cgcgtataag aaaagtaatc cttccgaatc aggtttccct taaaaacata ctcggcatgt
     3001 tgaaccaagt ccttgttctc atcaaccaaa taacaggtta caaacttatc ttggtcaaaa
     3061 aagaatacac gtaaaacctt gccatttttt tctctgtgac tttcttcacc accaaagtaa
     3121 gccaagacat catccactgt cacgctagta ggtgcaattt tgatatctgt gaaatgatta
     3181 taaagccaga taacctgatt atcatcaaaa ccaatattgg ctgttaagtg ctgaatatta
     3241 tcggctaaaa tcatatctgt aaagataaac ttagaggaca gatttaattt ccgaaaaaca
     3301 ccagcacgat aggcttgagc gtattcaaca ccgctactag cccaaccaat tcctaaattt
     3361 atattgtaaa ttgtcatatt ttctcctctt atggaaaatg agtcacgatt tcaccttttg
     3421 gagtgacctc aaccagcccc atcaggagat tacgcaccat atccgcacgg atttgttctt
     3481 tcatagcttc aaagcccgcg taggcttctt gatagtactc tacgattgga tttttctgac
     3541 tagcagattg accaccgata gccatggata gctgttgtag atagtctacc tgctctaccc
     3601 agttgtcatc aatggcttta agcagtgaaa gtcgtaaaaa ctgttcatat aagtcatgtt
     3661 gattaagtaa ttctttcttt tcagaaagtt ctttatcaat cacctgcttc ataaagctac
     3721 gaactgcagt tttgtcagtt acatctatat aatctggaac ctctttaaca tgaaaactaa
     3781 tattggtcac aataaagtga aacaataatt cacgactagc atagtgatca gccgctacct
     3841 cttctgtata tctctcaatg atatccacaa caacatcctc taagtcacga gaaccatcta
     3901 ttagacgatt tctctcttta tagactatat cccgttgaat attcatactt tcagcatatt
     3961 ctagagtctg acgacgtgcc gaacggccag cactatcact ggcatgctga gccttttcga
     4021 ctagtttccg gtatttacga cctttcaata cttccggttg agtcatatct tgaacctgat
     4081 agtctttgta ctttttatgc acccaagatg gaccaaattt cttgataaca tcatcctcta
     4141 aggatacaaa aaatttactc ataccaggat ctccctgacg accagaacgg ccacgaattt
     4201 gtaggtcgat ccgctgactt tccatccgct cagtcccaat aacaatcaag cccccaagct
     4261 ctgcgactcc tttaccaagc ttgatatccg taccacgtcc tgccatagag gtagccactg
     4321 tcacagcccc catctgacct gactcggaga taatctgagc ctcacgcgcc gcattattag
     4381 catttaggac attatgggca atcccttcac gaaacaagag agacgaatag agttgagaca
     4441 tttcaactga gcctacaaaa acgagtaagg gatttccctt agcatggtat tgcttgatgt
     4501 actccaagga tgcatacact ttttcaggta aagtgatata tagattatct ggatagtcaa
     4561 tccgttgtct cggacgattg gttggaatgc gtactacaga catattgtaa gtttcaataa
     4621 actctttttc cgcgacctta cctgtccctg tcataccaga tatcttatta aacatcttaa
     4681 aaagactctg ataggtgatc gaggccatag cccgcgtctc aggagataat ttgacatgtt
     4741 ccttggcttc aatagcctga tggagacctc cttgaagttt agtcatttcc attagacgcc
     4801 ctgttccctt atcaaccagt accatctcat ttccacgaat gatatagtcc ttatctttag
     4861 taaagagctt atgagctcga atcgcataaa ccaaatgacg agcaaaagac gcatgctctt
     4921 ccttgtataa attatcaatc cctaggaaat tctcagcaga cttggccccc ttagtagtga
     4981 gccaaacctc ctctttctcc tctttaaaga tataatcctc tccttcgacc aaggttgtta
     5041 caagtgtatc aatgatcgca tagtaattag actgaacacg aggagaaccc gcaataatca
     5101 gaggagtttg tgcactatca agcaagatat catcaatttc atcaataatc acatagttaa
     5161 acggtcgtaa aaacttacct tcttcattcg aggctaggtt atcatttaga taatcaaaac
     5221 ctaaattact attggttgtg tagatgatat ccgaagcata gataagcttc ttttcttcag
     5281 ctttcatctc cttctttgga tcttccgtaa atggtacacc aatggtcaat cctagaaaac
     5341 gataaacttg acccatttcc tcggcatcac gctttgataa atactcatta ggagtcacaa
     5401 ccatcactcc ttctcctgaa aaagcgttca aatagacagg catggtagct gtcaaggtct
     5461 taccttcccc cgtattcatc tcagcaacat ttccatagtg catgacaata gctcccatga
     5521 cttgaacatc ataaggaaac atccctaaaa tccgcttatc tgcttcacgc accacagcaa
     5581 aagcttcaac caaaatatcg tctagacttt ctccctcgga aagacgctga cgaaactcta
     5641 ctgttttagc tactaattct tgatccgaaa gagaggacat cttgcctttc agggcattaa
     5701 tctgctttaa aatctttttc actttcctaa gctggaaatc ttgacctaaa cgtctaaaca
     5761 cttctgatct ccttgataga aaaatgataa aaagttaagg actcaaatcc agcacttaaa
     5821 agagaaactt gataagtata actatcttct ggataagtaa atatgaaatc tttcactttt
     5881 tctacctttt tttctaaaat atcaccatag cggtcaaaaa aagttatctt ggtataaact
     5941 ccattttcag gctgacaatc aaaaaccagt gatagttgat actcctgctt tctttttaat
     6001 aagggaagct gaggttgggt tcgtgcagcc tggtaattga cactagaaaa ccaagtcttt
     6061 agaatctcac cagatggcaa tagaggatta tacagacgaa cacttttgtc agggtaataa
     6121 gatacagttg ttccatacat ataagtccca ccaacttctc cccaatgaat agactgtctc
     6181 tgtgtaataa tcatcttttc tcccttccaa aaccagtctc taagaccatt ctataaaagt
     6241 gcaaaaacca agaaatattg gtatctgtat catcattatg tcgtccagca gttcctttag
     6301 ataaaatctt cgcacctgta ttacacagat aagacactaa ttgttcatag gcttgtggat
     6361 ccatttcttc atctttcata taggataaac caaaggttgt ctttgaaaaa tttgcttgtt
     6421 taaaaatttt ccaaaaacgc tgatccaact ccgtcatatc ttgagaactt gtcccacctg
     6481 tatgatgaat taaacaatca aaagctaagt tagagacacc cggtgcgtcc aaacgtccac
     6541 gactagctat agttcctaaa ttagccaagg gcttaccaac tatgatggca tggggttcaa
     6601 aagaagcacc ataatagaga gccggaaacg ttcccataga aagacctgat aaaatcaaat
     6661 ccttatgatc aaagcctaaa taatcaagat agtgagtgat cgtatccttt acttttccct
     6721 ctagctcatc cgttccgaga taaaaagctc ccccctctaa acgtggatca gaaaataaaa
     6781 tgaagggaca tccaagagtc ttcatcataa agtaaccctc gaatccttct gcaggtcgat
     6841 aacctgcaaa atagacagtc aaaggcggtt taaaatcacc tggatggaag aaatagttta
     6901 tttcatcacg cttggaatca tgtaggatat ttccaccaag tacaaactta ccaaattgtt
     6961 ttcgactcca tcgttggtga agattaccta gtttgatagt tccacgacct cttgcttcaa
     7021 cagatatcga gagaaagtag tctccgtctc gactatccat aaaaagtgct tgctccaagt
     7081 ctttttcaga tactattact tcttcaaaga aatcatcaac agacccactc cacattttac
     7141 ggattactaa acgaaagtca caattgcctt ccttctcata ttcaagccac aactcaatag
     7201 ggagtgtttt ttgcaccata atattataag cccaataggc aagctgggca aaatcttgcc
     7261 caaactcacc ttccaaactg acataatcca atccttgata agaaatagct cctgtaaaat
     7321 ttggattgac ttgtatcgtc ggaggaaaga gtttatcacc atacccaccg ccaaaaagag
     7381 atttacttaa atcattgatt agttgttggg gacttaaaaa atcaattgct tgtgcacatc
     7441 gtttttttag aaaatccaca acctctctat catttatttg tagattctgg ttataaaata
     7501 aagtataagg ttcaaaatat tttacatagg aaaataagtc tctggaatac tgaccatctt
     7561 ctattaaaac tgcatgaaaa cggttaatat cctctttttc aattatttca cgaagggctt
     7621 ttgatgagtt tgggtaaaaa taataccaac tcatattttc aggtatgtca tagtgatttt
     7681 cccaattggc aagtcctatc tgtaaaatat taagttcctt tgacattttt aacctcctct
     7741 atccatttct ctaattttcc aagaaattgt tgtcctgtgt gctccttaat cttatcaatt
     7801 gaatatatca aggactcatt ccactccttc aacctatctg tgtaataatg tgcagcctta
     7861 gaaaattctg taacatctgc taacaaataa ccatttttta aatgttcaac atagacggtt
     7921 tcaactaggt tgatttgagg aattcctgca ctaatcccag caatctgtgt gtagagatga
     7981 ggatgtctat ttaaatccac aatcaaacgg acaaattcta gtgtttttat caaatctaac
     8041 tcatcattca agttaacaaa ctgtaagcgt aagtcctggt gttgattttc ttccagagga
     8101 ttttctgcat caccataatc aatcgccttt cccagatttt cggattgaat gttttcttgg
     8161 atgaaagact caacaatccc ttcaacctca ttcatttgct cctgactagc agcaaaggct
     8221 ccaaaaatca cctcagtatc cttattttca gcgacaaagg acaagacttg aagcagagct
     8281 tgattatcaa tcccctgctc aaaatccagt tgaaaataaa tgatggattc tttcttagtt
     8341 tggcttcgtc ccaatcgtaa tcgggtgtca aaagatgata aatgataaca ttgatgcatt
     8401 cgttcaggat acaactcctg caacaatcgt aaactatcct ctctatccac caaaatcaaa
     8461 tccgattttt caaaagttac atctaaatcc ctaaaggtat cttgaggatt acgtccaatg
     8521 aacagactca acagtttagg attagtacta ggtaaacgat ctagtaccaa ctggtcatga
     8581 tgagaatgag aaggaagcat aaaaatatcc tgatccttca catacgtttg cagataattc
     8641 tcaaaaaatt ctgccaccag atctcccata ttttgatagg taagcatttt aaagcgataa
     8701 cccaaaattg gattgacttc tacctgtcct ccttctttta aacgctctct aaattgccaa
     8761 actcccttag gattcaaata ttcttgatag gctgcttgcc catcttcaaa aaaaataata
     8821 ctcgatataa accctcgatc atccatgata tggtgcatat gtatttgacc attcggtcca
     8881 aaatactgta tatcactgat gaaaccttca actccatgtt caaccttagc aaatttcttc
     8941 ccattttttt gaacgataat cgtaaaggga ctataaataa attcacagtc atcatcccac
     9001 tcaatatctc taatgcttaa aacttgggta tggagattat gaaaatcttg cataacatca
     9061 aaaacggaat aagtatccgt ttctaacaca ccatgtctat ataaaaaata acgtagatga
     9121 ggctggtaag ctaatactaa tagacgagaa tctatatctt gctcttgaaa gagccgaatc
     9181 tggtgaaagg tatcatcaaa ctctagacga aaatgagaaa aataccatgg agtgatatct
     9241 gcatgccatg ttctttctga cccataccaa gctggaataa aataatacat aaaagtctcc
     9301 taaatcaaag gtctataggt ctcatttagc cttaaagcct tgacctcgtc tctaatcgta
     9361 aaaatcatgc ccccgaacat cataaataag cctggaatca ttgccaatcg taataacttt
     9421 tcatcaaaca aaacaaaaag catgggacca cctgccataa tcacattaaa gagaccacct
     9481 atgactgaga aacgaaggac caatcgatta ataaatcgac tagtatccgc acctggataa
     9541 ataccataaa tgtattctcc agattttttc atacggtctg caatctcttc tccattcatc
     9601 gtaacaaaag caaaaatgat actaaataaa aataagaccg aaatataaac atagacccac
     9661 aaaggcttcc caaccataaa ttcctttgat aaagccgcta gccctgaatg attagggaaa
     9721 ataaatccca acaagatgaa caaataagct ggtacactaa gaaaactcat cacatacata
     9781 taaggcatcc cacctgcagg attcaacatg atttcgagat aagaatagcg tttaaatcga
     9841 ttgtgtaagc caattttatt aacaggaacc aaatagcgag ctcgatacat aagggcaagc
     9901 aagtaagaaa atataatggt cataaaaaca agcaagacaa caatccctgt agggatataa
     9961 accagtttaa aagtttcaat aacatcctga gggatattta aaacaatgct ggataggagg
    10021 attacaatag aacccccaat tcccatgcta gcatttaaat ctgacaacca aacaagaaaa
    10081 aacgttcctg ctatgagtaa caaggtattc attagagcaa ccaaaattac cgagtaggag
    10141 gattgtactg gcaagctcaa gctaactgcc aaggattgaa tcacagcaat tagcaaggtc
    10201 agatacattc ttcgacgatc ttgaatttct atagacgtag atgttaaacc caaccgttta
    10261 gaaaaagaaa acatctgcca taaaatcatg gcggacatcc aaggggataa tccaacagaa
    10321 aaaattgata aacttcttag attcccacct gttagggcgg ctgagaaggc tagataggct
    10381 gttgaacctc ctaaaaaatc tttagtattt aggtcaacaa aagggagagt aatacgactt
    10441 cccaatacat agataaacag tagaaataag gtaaatagtc ctttttttac tgctattgac
    10501 gaataaatct tagtcataaa acactcccct caccttcatt gtttgtgata tggtaaataa
    10561 caattctttt atttcaactt ttaccatttc tttgttctgc atcaaaaaac aactttaatc
    10621 tgaaacaaat atctttatat tcctcagtat ctgttaattg atggtattct aaagtttgct
    10681 tataatcttt taaaagattg acaaatcgaa tacgcagaac ggagacatcc aaaccagcca
    10741 agacacaatc cgatattttc ttagaaaaca cttcaacaat atctcttgcc caggaaagat
    10801 caaattccgt tctcatgatg cttcctgaac gtcttctata cagatagaga ttatcgttta
    10861 taaagacgat tttagaggct aaaagataaa agcgatgcat agttgcttca tcatcaaagc
    10921 ggcgaccatt tgaaaagtga acttcattga ataattcttt tttaatcaac ttaaatgtcg
    10981 gcaatataaa ggccgagcta ttgaatttcc aatctcctgc ttgacggttc ataatttctt
    11041 gtatagctaa ttcttcgacg caaaaatctt tccctgttac ataaaaataa aaattcccgt
    11101 cactttcgtc ataattatag taattcccta taacaacatc tgcatccttt tcttggactt
    11161 gctgataaag agtttcaatc atattttctt ttacgatatc atctgaatca acaatcgtga
    11221 tgtactctcc cttagctaca ctaatcccca tatttttagc ttgtgctgcc ccccccctta
    11281 ttttcttgat gcaatactcg cacacgatta tcttgcatca taaaagcatc acaaatttcc
    11341 ccagaattat ccgtagaacc gtcatcaact aaaataattt ctatattttg ataggtttgc
    11401 ttcagaatat gctcgacaca ctccactaaa tattttcccg tattgtatat cggtaccaca
    11461 acactaatta attcactcat tactctatct ctatattctt cattctatcc actaaatcat
    11521 ctggtctctc cacagaaaaa ataccgtcat agagtccatc atccatactt ttacgtgtga
    11581 tatcaaaagc gaagattttc ttgccacttt cttgtgccct tgtaactaca ttaaaaacct
    11641 ctccacctgt attaatatct aaataaacta tagaatcgtc caatagccaa tcaattctac
    11701 tatgtaatac attttgatat actgttacat tcgtatactg tgatagagag gtcagctcct
    11761 cagaacaatc acacggtgca gccaaatgaa aatgccaatc aggtaaccgt tgtactaaat
    11821 actctaaatg ttttatatct gcactccatg tcacaagcat cacttgtttt ttagactgat
    11881 tgcttctttc aaaataattt aaatcggaac gttgaaaagc aatctctgac caatccaaat
    11941 ctctataaac ccaccataat tcacgtagtc tagatatact atatgtattc caaggtttat
    12001 catgactagc atagtgaaca attgtaggtg gattgtcatc tagacgttca cattcttgag
    12061 caaggtgata aatatcaaca cctaccatat aattatatgt tttatctaag gctagccaat
    12121 tatcctcaaa ataaatattt aaaatactct gatccccaag atgaacaact tgattttgct
    12181 cggccgctaa ttccaataaa ctattgacaa tagagtgttc tttccatttt gcaacatcca
    12241 ttaataaaac tccagaatta aatccagatt ttcgtccttc ataggcatag acatcatcaa
    12301 cagcaccaat tgaatatcct ttgagatcta tctcaaacaa agtagctagt tccccagtta
    12361 caatgatatc ggaatccaga tacaataccc tatcagattc aaccacttct gtcgcaaaaa
    12421 atctaaagta agaagcataa tttatatgag gtcctgtttt atagctttca aaaagttctt
    12481 tatcaatgtg tacactgcga attgtagaat tcacaacttc catttttctc cccaataatt
    12541 taaaccattc aggagctata tcactgttga gaatataaaa atcaacatct ctattgtgat
    12601 acaatacaga ctttatagtc gtctctaaag gaataagata gacattatct gccgctaata
    12661 ctattgattt tctcatactg ttctctacct catgtaagat ctgattgctg caaccatttt
    12721 tttcggaatg gagcttggat agataccttc atatgaaatc tcagaaagat tttcagagca
    12781 agtattgtcg aaagctatca ttggtttttt gtacttaagc acaaactcat atgcatcttc
    12841 taattttcga tcatgattca aatccaaata caaatctgaa gctattacta tttgttccaa
    12901 taacattggt aaggaatttg gatatacggt aacattagga aaacgcgtta aagctagtag
    12961 ttcatttgcc atatttgtat aagcaacaat atgaaaatga acttctggca attgctccgc
    13021 caaataatcg attttctcga cacaccaaga attcgttaaa ttaacacatt gcaacttaaa
    13081 tatttgtgat ttactaggat acttcacact ctttgaaaac cattcattta aaataacaga
    13141 ccaatccatc aaagagtatt cccaccaaac ttctcttaga cgtccaacag aaaattgatt
    13201 ccaaggctta tcctgagaaa tatagtgtaa aattagtggc agtggttcga gcggaatatc
    13261 aaaaatgaat tgatgtttaa aggctgccgc cccataatca tatcctattt gaaaattata
    13321 ttgatcttca agggaactat attgatcttt aaacaacata ttcaaaattg actggtctcc
    13381 ttcttccaca ttctcatgtt ctttttctgt taagtcaatc aatttttgtc gaatagtttc
    13441 agatccccat tttttgttgt taatcaagag aacaccagca ttgaagccga ctcctgctcc
    13501 aaagcaagaa cgagctgctg ccaaataatt ttcacctaag tctaattcaa acaaatcggt
    13561 caaatcacca gtcacaatca aatcactatc tagatagaga actttatctt ctgttacaaa
    13621 atctggaata aaatagcgtg caaatgtcat atgattgata tgaggtaatt tattagacca
    13681 attcatttga aactgtgagc caattaactt gcagtcaatc aagtcgcccc ccatctcttg
    13741 taaatatatt cttatttgac taaaccattc ctgaggaatg tcctgattta gcagataaat
    13801 tttcaaatga ctattgtgtc tacagagtga cttcatcgcc gtttcgattt gtcgaatata
    13861 agcgtaatca cctgcaaata ctacagattt cttatcttgt aacattttat cgtctctcta
    13921 tcttaatttt cagttatatt tattaccatt caataaatag tctaacattt ctaaatgtct
    13981 tgtttccaca ttctccttaa gttctcgttg aattgcaata tcatgacgat atttcaaata
    14041 gaacttcatc tggtctaaat atttactgac atcatatcct tttagcgcta gcacggcaat
    14101 tcgttctaac ctaaaatcac aatcatcacg catcatcttc ggtgaataag aattagaaag
    14161 tccttctttc ccaaccctat accaatacaa aactttatta atataggtta ctttctcaac
    14221 ctgcataaat aatttataat tggttcctat atcctcacac acccgaccaa acggaaactg
    14281 aacttcttga aataattccc ttttaaatag aattccccaa gaagttaaaa agctcatatc
    14341 atacctctct aataaaggaa gttcttccat tagaagtttt gaattataat ttttcacata
    14401 ataatcggag tcaaatacgt gaatatagaa taagccatct gactcattat aggttgaata
    14461 agttgaaaca acaatttcag aatgctgctc cttcaaagtt tctataagaa ttgataaata
    14521 ctcttcagta acccaatcat ctgagtctac aaaagtaata taatctcctt tagatctctc
    14581 gattccaaaa ttacgtgcat ccgctacccc agcattttct ttttcgaaat agtgaaaacg
    14641 actatcacga tcagcatact ctcgacatat tgctcctgaa ttatctgtag agccatcatt
    14701 tactaataaa acttcaaaat tttgataaat ctgtttcgat aaactgtcta agcagctaga
    14761 aataaatggt tctacgttat aaactggtac tataacactt attaaatctt tcatacttct
    14821 ctctttgata aatcaactaa aatctgactg ttataggttt catcctcaat gaactgaata
    14881 tcttgatcaa aattatactc tgctgtatgt ttatgtgaaa atttaacctt agttttagca
    14941 cccatcttca tccactcata ctcggagtca atatgcccaa tatctaagac ttggtaaccc
    15001 tttcgactta aatgataggc taaaactttt gctgtaggac ccagcataca taaaatcagt
    15061 cttccttcag catgtttcat aatttcctct tgaatgttat cgacaacgga ataagcatta
    15121 tgagatggac aaataatccg tttcacagat ttgaccttat caaataaatc atttccaacc
    15181 ccagagcgag atgtaatccc ctctacaatc agaatatccc tattctccca aatactcttt
    15241 agcttttcaa attgtccttt tgccttactc ttatcttcat aatcaatgta tggacgcgag
    15301 ataaaggtag agccatacca tgaaccctta cacaactctt tataaaaatc catataaaca
    15361 ttcatatgtt ctttccagaa tggtgtagcc caccaagtaa agacaaaacg atcgtcaaaa
    15421 gcatcaggta aacaaaccac taatttctcg tcactctcaa gacccacaat atgttttaat
    15481 tggttagcta actctagatc atgattttga taaggtatag gcaaacccca catcaaattg
    15541 acttctccgt ctccaaaacg aactaaagaa gagttgtgtt caataatata atccaaggtt
    15601 tcatctatcc ctttcacctg gattatatca agcggttttt ctagcatatc cttagttatc
    15661 aatttttgca aatcagccaa cattcgttca ggatgttcac ttgaatacaa cctttggcca
    15721 ttattcccat tttgagattt ataaaaggct agtataggtt tagatagatg agccatttct
    15781 gcaacgatat cggatgttga agtcagtagg ttgatatcta aataagcatc catcttctcc
    15841 ttcaactcgt ctagcttatc tctagaaatt gccggataga gctgaatatt aggataacga
    15901 ttagacaaat catataattt tggccccata gctgtccaag cagcaacttg aaaaacaaca
    15961 tttggcaact gtccagccaa gtaatccaat tgttcgatat cctgagaagc cgtaaacgtt
    16021 aaacaagtta aacttggaag ttgtcgaaaa caggtaagat gtccgttttt cttaaaatct
    16081 ttaatcgtat caatcataaa ggaaactttc ccattttcga aaacgatttg tcctaactct
    16141 ccgtgctgag tcgattgaaa agctagtagc gttttttctt gttcttgcaa agattttaaa
    16201 aagttctcat ctgcactacc atgattgata tccaaatata gatttgttga ctttttcagc
    16261 ttatctaata caggaggaac aatttgtgga tgcaatctca cattattata tacagctaat
    16321 ttttttaatt tatctcccat atccgtccaa gctgcgatat gaaataccac ctcaggtaga
    16381 gctgtaacta gctcttctat tccttctaaa tcttgggaat tcgttaaggt taagcaagaa
    16441 aattccttat ctagaggcga tattagttca aattctccca aatgatgttg taaaatctga
    16501 ttccactcca aatcatggaa ttcccaccat aaatcacgat aacgattggc tgtcaaggtg
    16561 gtccagggtt ttctgtaggt cgtaaaatga atcaccactg gtttatcatt aaaagccaga
    16621 tgttcctgcc aattgttata caaagccaca atatcatgcc ctacttgtaa attataagct
    16681 cgacctagtt ctaaccaatc atcttgtaag acctgattaa aaatcgtttg atcaccatta
    16741 aaatgctcga aacggccttc ttcaacttcc ttcattgtaa caatgctctg ttcaattagt
    16801 cgttctttta atttctcttg acgccatttt tcattgttga ttagtagaac accagtatta
    16861 aaaccttgac catttgcatc tctaatcgct gcgatataat gttctcttac atcctgctca
    16921 aatagactat ctaatttatc attgataagc aagtcacaat ctaaatacaa aaccgtatct
    16981 tcttgtatat aatctgggat aaaataccta gcataggcaa ttgaactaat gtgagcctgt
    17041 tttttccaat cttgaaacac agtttgttca ggtagtttaa catcgataat ctcactacct
    17101 aacatgctag ctatttttcg tggctttcga aaccaatctg gcatgatatc ttgatttaaa
    17161 atataaatct taacatctcg attgtggtat aaaatagatt ttatggttgt ttctaactgc
    17221 ctggtgtaat tacgattacc agctaataca attgttttaa tcatcttgtt ctcttattca
    17281 actcccaaat ggtaaattgc atctaccaat aattttttag taaaataacc ttcttttagc
    17341 aaataactaa aggtttttat ccgattcgtc atttcttgat attcttgtag attcatttta
    17401 tcaactatct catgaacttc ctctagacta ttcgccacaa agcccaagcc ttgatctact
    17461 ataaatttag cagttgataa gttgctcggt acaatcactg ggataccagc tgttagatag
    17521 gtactaacct tatgagatat attcaaggta tagtattggt tactttctcc ctcattttga
    17581 tgggttcccc agacaaggcc aaatccaccc tttgataatt ctagcaacaa ttcctcatct
    17641 tttttccatc cttcgatgct gagatttcta gcactagaac cggcctcccc tttatttgaa
    17701 aatactctca aaggcgtatc ttgagaccaa ttttgtaagt ctggaaaacg ctctaaactt
    17761 ccagcaaaaa aagttctttt tttaaaagca ggggtgtata aggataaatc atgaggatga
    17821 tcccacatcc cttgaacaag aatcttctta gtcgtcaatc cttcttccat caggcgtgtt
    17881 ttcattctct ctgacggcac tatcaaaaca tctgatagat tatacatgta catatagtct
    17941 ttcatgagat aatagttact atcaaacatg aggggaacaa catcatggat aaagcaaata
    18001 attttcacct gcatatcctt tagcttatca aacaagagac gatcaaattc aaagccattc
    18061 caggttggag actgaaagac taagatatca ccaatagaga tactggccat aataccatcc
    18121 agacgcttat tcatttcaga aggactatct gaagcaatgt tgtaaaaata aataccaacc
    18181 tctctaaatc ccagttgact agctatcttt tgaacagcat tttgagctaa gataaccgta
    18241 ctatcaccag ccatgccgta taaatttgtt aaatgtaatt tcatattacg ctatctcatt
    18301 ttttattact atctacggaa gtaaagacaa tttttccatc attcttctat aaatctctgt
    18361 gaactgcata ttttgttctt ctaattttgc tactactctt tgtaatctat ttttataaat
    18421 caaaatatga ttggtcaagt catagtctga acttgctaaa atagctatct tttcttccct
    18481 agccccaatt tcatcataca tacgcttttc actccatgta ttcgataaag tatcagataa
    18541 accaacacgg taccagtaaa cacaacgatt caaatagact atcctcgaag ctcttagaag
    18601 taccttccat gtccagtaag tatcctctgc aatttttcct ataggaaatg gtaaatcttg
    18661 tagtaactct ctcttgaata acttcaagac agctacagtc cagttcccat ttctgacttc
    18721 ttcgacacct tccctatcca taattgcttt accttctatc acttctagag aatcatctgg
    18781 atccgtaaca taagtcatat acacatagcg tgtttcatca taagaattat aacgcccgat
    18841 actaatatct gcgttttcct ttttcaaagc accatataat cggtctaaag catcatgttc
    18901 caaccaatca tcagagtcta caaaagtaat gtacgccccc cccgaacatt caatacctag
    18961 gttacgagct gatgaaagac cgccgtttgc tttctcaaaa tatttgaaac gagaatcttt
    19021 ctctacaaat tcttcacata ttttggatga atgatctgga gagccatcat tgattaataa
    19081 acactcaaaa ttttgatacg tctgattctg aatgctatcc aaacacattc tcagataatt
    19141 ttccacatta tatattggaa caacaatact gattaactca ttctccattg acttattctc
    19201 ctaaaaattt gcgaatagca tctaccatct tatctggctc agttgctgaa aaaatccgac
    19261 ttcttccttt gctatcatga ctagtattat caaaactaaa gattggctta ttgcgcctac
    19321 agacttcttc tataatgttg acaatctctc cttcatggtt aatatcaaga tagaaatcca
    19381 tcttatccaa ggtttctttc atattaaaag aattaaaatt tggaaaaagt gaaagattca
    19441 gatatctctg taaatcaaca acagagggag caaaatttgt atgcgccaag atcgtaaagt
    19501 gcacttctgg caattccgat aggagatatt ccaaggactc cattgcggca ctatttgtaa
    19561 aaatacaagt atgatataag ggaacaccca ctaactcctt aaaagagcgt ttaacgatat
    19621 ctgaacttaa caacacatcg ttccagttca atccataata aaaccaccag agtttacgaa
    19681 aacgtgtaag tgttaaatga gtccacggtt tgaactctgt agtataatgg acaatcttag
    19741 gctcgtaatt tccataatag ttggattggt accagtcagc attacctttt aaatgtaaaa
    19801 actgatctaa accaaccatg aaattatact ctttgtccaa atgtagccac tggtcaccga
    19861 aatatatatt gagaacacct tggtccccat agacatgttc atggtgttga gcagtcaact
    19921 ctagcaaatt ttgtgttgat tttttttctc gccatttttt tgcatcaatc aataacatac
    19981 ctgaattaaa tgttgttaac caaccatctt gatcaaaatc ttgaacagct gcgatagcgt
    20041 atccttgcaa atccaaaaaa aataattcat ccaaattggc attcacaatg atatccgagt
    20101 ctaagtagag tacccgatct tcaagcactt cgtctgctat gaaataccta aagaaagttg
    20161 cataggacag gattgcattt ggcaagtgga aatcacgaag attgtgatct gcttttttga
    20221 cattaataat ctcagaacca ataaccttca gacgatttct catcaaaaga aaccattcac
    20281 taggcagatc atcattaaac acataaaatt tcacttcgtg attatgaact gaaactgact
    20341 ttataacggt ttctacctta tcaagataat gaatatcagc tcctaaagca ataacttttc
    20401 tatacattct tatctcccga tttttctatt ttataaacaa atcatctatt ttaccaactg
    20461 aaaaaacgtg atttctactt ttgagatatt gattttttac ttctgtttca tcaacaatta
    20521 cagtatcctt ttcttcgatg atatcttttc atttctaaat ctaaatgttg aatcaaaaaa
    20581 tcttcaaatc aattatgcta tgaataagat ttctttcgtg ttctgcaaaa tttacaacta
    20641 tctacttata gattcggcat tatcaaatag attaaagact caccattaag atatcctctg
    20701 aagtcatgtt tggttttctt ccaaccttag aaagtttcat ctaacttgat tttatattac
    20761 tcattaaact ctcatcttta caaaaagatt tccattatta taaactacac tgtttgatgg
    20821 aatatcattg cgtatgagac aacctgctcc aataatggta ttatctccaa tcgtcactcc
    20881 ttttaaaatc gtgacattac ttcctatcca gcagtctctg ccaactctaa tcggcgctgt
    20941 agtccactgc cacttttcaa tcttttcagc ggtataaata tggtcgtgat cataaaaacg
    21001 aacaccctct cccatcatgg taccagctcc gatttcaatt ctttccatac agttaaaact
    21061 acaagagttg ttgataaaaa caccattatt caggattaac ttaccagaag agacatgaaa
    21121 attttcaaaa tttctacagg cgacattggc tcctacaatg aagtgagcct tatcatcgat
    21181 tttaaagaca ttccaagtcc catcttgaat gattttctct tgatgattgg tgcctactct
    21241 gacttcattc agatagtctc tcactctaga aaccaatttg tctggtgtac ttgatagaaa
    21301 aacttcctgc ccctgatttc catggactgt attgtcaaat gcaaagacag attttccagc
    21361 ctgtacaaac ttggagacaa tagcatctac ttcttgaaag tgattgatat ctagtaaaaa
    21421 gtcacaggta tcgactaaaa agtttagctg ttcttcattt gtaatcgttc ggagacggac
    21481 attatagttc gtttctaagc tacggatttt ctcggaaaat tgaacaggtg ctgcaatatg
    21541 aaaacagata tttgacaagg attcaaggag ataatccaac tcttcaatct gatcactaac
    21601 cgtcaaaatc agagcatctc ctactaaatg atttttcttc atttgcttat ttctctcaat
    21661 ttctcaatca ttgcttgaac ttggtcaaca gcatatgcct cctgacctac ttcataggtt
    21721 ttagtatttt caaaggataa gataggcttg ccaagattag caaattgatc gagaatttct
    21781 tctgtctttt cgccatgatt aatatctaaa agtacttgac tggtttctac caattcatta
    21841 tcgacatcta ccaaatagtg aattccgtta aatatagtca cgtttggata aattgtcatc
    21901 tgagccaatc gatcactaac tattactcta gctgctatct taaactgaat atcaggcaag
    21961 gattgaacca atgtctcaat ttgttcaata tggtctgagg cagtatagat tagacaagtg
    22021 aaaggttcct ttattggata gatgtgagat ctttgtaatg gatgtaaatg atggttttgt
    22081 cccaattctg tccattcaag cccatgatag taccaccaaa cttcacgata agtttgggcc
    22141 gccaaatctt tccacggttt tctatgggaa aggtagtgaa taatagcagg ataatcctga
    22201 ccctcaggca attgataatc agcaaactgt ttatgaatga caatatgatt ataatcaaaa
    22261 tccaattcca gccatttatg ttcaaaaagc atattcaaga tgctctgatc tgcctgatcc
    22321 accttatcat gccattcatt ggttaaatca attaattttt gggtcatatt ctcttttttc
    22381 caaaaagcat tgtttaccaa gagaacaccg gcattaaaga tttcttgacc aaaataagct
    22441 ctgcccccaa aatctctaac agcagccaaa ggataatctt gtaagtctgt agcaaacaag
    22501 tcatccagat ttttcgttac aactagatca cagtccaagt agagggcctt gtcttcttgc
    22561 acgaaatcag ctatgaaata gcgtaaaaag actgtgtaac taatatccga tttataacat
    22621 gaaatttgct cagaagttac ccgacaatta ataatttctg agtcaaactt ctctaatcgc
    22681 ttatttaatt gtttgatcca ttcatttgga aaatcgctat gaatcaaata aaaacgaagc
    22741 gagcgattgt ggtaacagat agacctgatt gtcgttaaaa cttggtctac ataggcataa
    22801 ttcgccgcta ggacaatggc tttcttttca cttgattctt gtaccgttaa ctgatttaaa
    22861 agtctttttt tcatttcaaa ctctttatac gttgctgtgt cagataaacc actagcttga
    22921 ccgttggcga gactgaattc caacatttga cgataaatcg ccaaatgctt ttctaaaggg
    22981 taacccatat tagctagtag cgtaatgcgt tcggacatgg catcaaccaa tgcatgcatc
    23041 cacttttctg tccaaactct tgataaacta ccttttctaa tccgattatt cttcatcatc
    23101 acgtttacgg cgtttcgcaa acaaacccaa gccagtcaca gctgccaaag ctccaaggag
    23161 tacagatgac ttggaagctt ctgtacctgt atttggcaat tgctgtcttg acttacctgc
    23221 tgaagttgac gcgcttgcgc ttgttgatgc cgattcagac gctgaagccg acgcactcgt
    23281 tgatgctgaa gctgaagcgc ttgtcgatgc tgactcagac gcactcgttg acgctgaagc
    23341 cgacgcactc gttgacgctg aagctgacgc actggttgac gctgaggctg aggcagacgt
    23401 tgatgcggat tcagacgcac ttgtacttgc tgaagccgaa gcgcttgtcg atgctgaggc
    23461 tgaagcactg gttgaggccg attcagaggc acttgtac
//
LOCUS       DAVMMR010000032        23338 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMMR010000032 DAVMMR010000000
VERSION     DAVMMR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.032"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            85..582
                     /locus_tag="VYS74_001223"
     CDS             85..582
                     /locus_tag="VYS74_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV3789269.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            582..2237
                     /gene="dnaX"
                     /locus_tag="VYS74_001224"
     CDS             582..2237
                     /gene="dnaX"
                     /locus_tag="VYS74_001224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV3789270.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2265..2459
                     /locus_tag="VYS74_001225"
     CDS             2265..2459
                     /locus_tag="VYS74_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV3789271.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2609..3379
                     /gene="sufC"
                     /locus_tag="VYS74_001226"
     CDS             2609..3379
                     /gene="sufC"
                     /locus_tag="VYS74_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV3789272.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3407..4669
                     /gene="sufD"
                     /locus_tag="VYS74_001227"
     CDS             3407..4669
                     /gene="sufD"
                     /locus_tag="VYS74_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV3789273.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4680..5906
                     /locus_tag="VYS74_001228"
     CDS             4680..5906
                     /locus_tag="VYS74_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3789274.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPLKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5893..6333
                     /locus_tag="VYS74_001229"
     CDS             5893..6333
                     /locus_tag="VYS74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV3789275.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6387..7799
                     /gene="sufB"
                     /locus_tag="VYS74_001230"
     CDS             6387..7799
                     /gene="sufB"
                     /locus_tag="VYS74_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV3789276.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFGDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8030..9271)
                     /locus_tag="VYS74_001231"
     CDS             complement(8030..9271)
                     /locus_tag="VYS74_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV3789277.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9374..10483
                     /locus_tag="VYS74_001232"
     CDS             9374..10483
                     /locus_tag="VYS74_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3789278.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11017..11364
                     /gene="ssrA"
                     /locus_tag="VYS74_001233"
     tmRNA           11017..11364
                     /gene="ssrA"
                     /locus_tag="VYS74_001233"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11547..11634)
                     /locus_tag="VYS74_001234"
     tRNA            complement(11547..11634)
                     /locus_tag="VYS74_001234"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11598..11600),aa:Ser,seq:gga)
     gene            11796..12536
                     /locus_tag="VYS74_001235"
     CDS             11796..12536
                     /locus_tag="VYS74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3789279.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12533..13444
                     /gene="pfkB"
                     /locus_tag="VYS74_001236"
     CDS             12533..13444
                     /gene="pfkB"
                     /locus_tag="VYS74_001236"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV3789280.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13441..15393
                     /locus_tag="VYS74_001237"
     CDS             13441..15393
                     /locus_tag="VYS74_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3789281.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15557..17782
                     /locus_tag="VYS74_001238"
     CDS             15557..17782
                     /locus_tag="VYS74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3789282.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17935..18363)
                     /locus_tag="VYS74_001239"
     CDS             complement(17935..18363)
                     /locus_tag="VYS74_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789283.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            18501..19643
                     /locus_tag="VYS74_001240"
     CDS             18501..19643
                     /locus_tag="VYS74_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3789284.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19652..20866
                     /gene="thiI"
                     /locus_tag="VYS74_001241"
     CDS             19652..20866
                     /gene="thiI"
                     /locus_tag="VYS74_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV3789285.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21001..21825
                     /locus_tag="VYS74_001242"
     CDS             21001..21825
                     /locus_tag="VYS74_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV3789286.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMLYFLGNLYL"
     gene            21838..23004
                     /locus_tag="VYS74_001243"
     CDS             21838..23004
                     /locus_tag="VYS74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV3789287.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
BASE COUNT         6855 a         4379 c         5161 g         6943 t
ORIGIN      
        1 aaatattata gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg
       61 ctcctttttt gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg
      121 ttaaatgaag aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc
      181 aacgccagtg ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg
      241 ttcgatggaa aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt
      301 gcactaggca agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga
      361 gatgtgacga cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg
      421 gtgccgatga tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt
      481 gaggattacg atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa
      541 aagacaacat gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc
      601 gaaaatatag aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc
      661 ttaaacaagc ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg
      721 gaacgggaaa aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag
      781 tgggtggcga accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag
      841 aagatgtcat tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc
      901 gtgataaatc tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg
      961 ttcacatgct gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac
     1021 agaatgtagt ctttattttg gccactactg aattgcacaa gattcctgct actattctat
     1081 cccgtgtgca acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc
     1141 actatatctt agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca
     1201 ggcgggcgga aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga
     1261 cacagggaaa tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat
     1321 cagccttgga tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt
     1381 gcttgaatct tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc
     1441 actatttaag agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag
     1501 tctttgtaga aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa
     1561 cagtgagttt agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga
     1621 ccgtccgttt ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa
     1681 ttgctacgct gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg
     1741 ttcctaaaca agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg
     1801 tcgatcgcaa taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac
     1861 gtcaaaatct aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc
     1921 cggacaaggc tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc
     1981 ttgcttttga gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca
     2041 tgtttggtaa tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca
     2101 tggaggaatg gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg
     2161 aaaaagaagt agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga
     2221 aggtagagga agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat
     2281 ttattattat ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga
     2341 ctggccgcta tattatggca gccttttggg caattttact ctttagaaat ttccgagtca
     2401 gttatgtgat gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc
     2461 cctcagcttc cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta
     2521 tatttataga atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt
     2581 aaaagttttg aaaaagaaag gaactatcat gtcagtatta gagatcaaag atcttcacgt
     2641 tgagattgaa ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat
     2701 tgccgctatc atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg
     2761 aaatccaaac tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga
     2821 gttggaagtg gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga
     2881 aatccctgga attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga
     2941 tgatgagaag atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct
     3001 taacatgaaa gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa
     3061 aaaacgcaat gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga
     3121 gattgactca ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat
     3181 gcgtggtgaa ggttttggtg ctatgatcat tactcactac caacgtcttt tgaactatat
     3241 cacacctgat gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga
     3301 attggctgcg cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga
     3361 ctacaaggaa gaattgtaat tccctcgtat cttttaggag aagtaaatga ctagagaaaa
     3421 tattaaactt ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa
     3481 agcttttgac aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg
     3541 ttggaatctg ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt
     3601 cacagcttta gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca
     3661 aactccagtt gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga
     3721 agaaattcca gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa
     3781 gttggcggct taccacacag cttactttaa cagtggtgct gtactctata ttccagataa
     3841 tgtagaaatc acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc
     3901 gtttaacaag catattatga ttatcgctgg taaaaattct aagattaatt atctggagcg
     3961 tttagagtca cgcggtgaag gaagtgccaa agcaactgct aatatcacag tagaagtgat
     4021 tgcacgttca ggtgcgcaag tcaagtttgc tgctatcgac cgtctaggtg aaaacgtcac
     4081 tgcctacatt agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg
     4141 tgtcatgaac gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag
     4201 ccatgctgac ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac
     4261 tcgtgtaact aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct
     4321 tgaaaaagca actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc
     4381 agatgcccaa caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa
     4441 cccaattctt ttgattgatg aaaatgacgt cactgcagga catgcagcct ctattggtca
     4501 ggtagatcca gaagatatgt actacctcat gagtcgtgga ttggataagg caactgcaga
     4561 gcgtttggtt gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt
     4621 tcgtgatgaa atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta
     4681 tgttagatgt agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg
     4741 aacctctggt ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag
     4801 ctattaacag ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag
     4861 cggaacgagc gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg
     4921 caggctctac aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg
     4981 cacgctttgc tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac
     5041 accattctaa tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct
     5101 atgtctatct taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata
     5161 aggttaaatt tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca
     5221 aggaaatcac tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat
     5281 ctacacctca tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg
     5341 gtcacaagat ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg
     5401 agcaaatgtc tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatctg
     5461 ctagttggaa ggaattgcct ttgaaatttg aggctggaac gccaaatatg gcaggagcta
     5521 ttggacttgc gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc
     5581 atgaacagga attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca
     5641 tttacggttc tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc
     5701 tccatcctca cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc
     5761 accattgtgc gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt
     5821 tttatatcta taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa
     5881 aggagttttt caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac
     5941 cattcgaaaa atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac
     6001 aatccgactt gtggagatgt catcaatctc tctgtcaagt ttgatgcaga ggaccgtttg
     6061 gaagatattg cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg
     6121 acagatgccg ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct
     6181 gaaatggttc aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg
     6241 tcaggtgttg ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt
     6301 aagaaaacaa ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta
     6361 ttagaaatga agaaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg
     6421 accttggtga atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa
     6481 aaggactcaa cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga
     6541 tgttggagtt ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg
     6601 gagcagactt gtcagagatt gactttggtg acttaatcta ctaccaaaaa ccatctgaca
     6661 aaccagcccg ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg
     6721 ggattccaga agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag
     6781 tggtttacca caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag
     6841 attccgcact caaggaatac ccagacttat ttaatcaata ctttgcgaag ttggtaccgc
     6901 cgacagataa caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct
     6961 acgtgccaaa aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg
     7021 aaaatatagg tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact
     7081 acgtagaagg atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag
     7141 aaatttttgc tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata
     7201 acgtctataa cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga
     7261 ttgatggaaa cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag
     7321 aaggagcgcg tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca
     7381 cgggtgctaa gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca
     7441 tcgctaaagg tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta
     7501 agaaatctgt ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag
     7561 atactattcc atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag
     7621 tatctaagat ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg
     7681 aggcaactga aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg
     7741 aatacgcagt tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa
     7801 atttgatttt atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg
     7861 tatggttact gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg
     7921 aggctagctt cctagtttgt tctttgattt tgagtattag atttactcaa aatcaaggat
     7981 tttgaagatg aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct
     8041 cgttaacaaa gcggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt
     8101 tcttgtctgc taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca
     8161 agtccttgtc ttcataagtc aaatggccaa ccactgttcc agcttcaagt ggtgctggga
     8221 ttgctttgga atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcga
     8281 ttagatagat atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag
     8341 gggctttgct atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag
     8401 aagaaatata atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt
     8461 gatgatctgc atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa
     8521 caaaagactc tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt
     8581 aagcaggcat accttctaac atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag
     8641 aagaaggttt tttggtgatt tctaagactt gtgggtattt tttgatgagg ttgcgagcaa
     8701 cgatagcgac atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa
     8761 tgttatcccc tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa
     8821 ttccccattc caagagtttt gcccgcatca tatcgacgaa atctttttct gagccagcaa
     8881 ttttctcagc tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca
     8941 actcttcgac agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg
     9001 tcaattgata aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt
     9061 ccaaagcttc atagaccaga taaacagtaa tcaattttgt tatggaagca atttcgacag
     9121 gttgcgttgc atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat
     9181 gtttagcggc aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga
     9241 cagttaacaa agttaaaaat atttttttca tagtagtctt attctatcat aaagaaaaaa
     9301 aatattcttg ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg
     9361 agggaggtaa ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact
     9421 cttaaccatc atcttttacc tatggagaca gatggggtct ttgattaacc cttttgttag
     9481 cgtgcttaat acaattatga ttccattttt attaggaggc tttctttatt atttgacaaa
     9541 ccctattgtt actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat
     9601 taccttgtgt actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt
     9661 gattaatcag ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga
     9721 cttaatcata gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat
     9781 tcagcagtta aacttatcct atgtggatat tcttcaaaat atcctaaata gcgtatcaaa
     9841 tagtgtgggg agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc
     9901 agtttttttg gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac
     9961 gattctaaag agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat
    10021 tgctcgctat attagtggag tttcgattga cgcaatcatt ataggttgtt tggcttatat
    10081 tggctatagt attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc
    10141 caatttaatt ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat
    10201 attcactgat ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt
    10261 agatggcaat atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac
    10321 gattttagtt ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc
    10381 agtgccaacc tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa
    10441 tcataaaata atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg
    10501 atttttcttt actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa
    10561 actaattttc acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg
    10621 gaattctgtg tcagggtaag ttccactggt tttcatagtc tattaaagtt cgaatgaaac
    10681 ctatttatag tagattgaaa ctagaatagt acacctctaa ttctaaaaca ttgttagaaa
    10741 tcgatttgac tgtccttatc tattcgttct attcttattt cattttacta tattttggtg
    10801 caataagtga aaagtagacc gaataatata aggattgatt ttatagtttt taaactcaaa
    10861 tgaattgaaa taaagagagt acgaaaattc tcatctgaaa gtattttaga ataattctct
    10921 tcgtgaattt cttcaaaaca gatagcttca tcttaggtat gtgatttctt tttgcatttt
    10981 tgagttagat aaggtataat ggttttattg tcttttgggg tcgttacgga ttcgacaggc
    11041 attatgaggc atattttgcg actcgtgtgg cgacgtaaac gctcagttaa atataactgc
    11101 aaaaaataac acttcttacg ctctagctgc ctaaaaacca gcaggcgtga cccgatttgg
    11161 attgctcgtg ttcaatgaca ggtcttatta ttagcgagat acgattaagc cttgtctagc
    11221 ggcttgataa gagattgata gactcgcagt ttctagactt gagttatgtg tcgaggggct
    11281 gttaaaataa tacataacct atggttgtag acaaatatgt tggcaggtgt ttggacgtgg
    11341 gttcgactcc caccggctcc attattcctt tgcattcttt tgcattcctt ggtaaaacgt
    11401 tgttaaatca acgtttttta tttttatctt tggtattcct ttgtattctt ttgctaaaaa
    11461 gggagtcaca aacagaccct attttaaaaa aaggatagaa aaaaggatac aacatttgtc
    11521 gcatcctaaa aataatcttt tttcgacgga agacatggga ttcgaaccca cgcacgctat
    11581 tacacgccta ccgcgtttcc aacacggcct cttaagcctc ttgagtaatc ttccaatact
    11641 tactcaaata gtctaccata aaggctctta tcttgcaata aaaattctag aaataagaaa
    11701 aatgatagat tttgaaagaa aatgataaaa aatgcttgac ttcgaaagaa agtatgatag
    11761 aatgaatagt gtaaacgata acaggaggtg attcagtgtt aaaaacagaa cgtaaacaac
    11821 taattttaga ggagttaaat cagcatcatg tagtttctct agaaaaatta gttagtttgc
    11881 tagaaacgtc agaatcaacg gttcgaagag acttggatga gttggaagcg gaaaacaagc
    11941 ttcgtcgtgt gcatggtgga gcagaactcc cccactcctt acaggaagaa gaaaccattc
    12001 aagaaaaatc tgtcaaaaac cttcaagaaa agaagttgct ggctcagaaa gcagcctctc
    12061 tcattaaaga aaaagatgtc atctttatcg atgctggaac aacaactgct tttttgattc
    12121 atgaattggt caataagaat gttacagttg tgaccaactc cattcaccat gccgctcagt
    12181 tggttgaaaa gcagattcca actgtcatgg ttggaggaaa cgtcaagacg gcgacagatg
    12241 ctagtatcgg gggcgttgct cttaaccaga ttaaccaatt gcactttgac cgtgccttta
    12301 tcggaatgaa tggtgttgac gatggctatt atacgactcc tgatatggag gagggagctg
    12361 tgaaaagagc tattttggag aatgccaagc agacctacgt cttggtggat tcgtcaaaaa
    12421 ttggacaaac ttgctttgcc aaggtagcac cactcaaacg cgctatcgtt atcactagtc
    12481 aagggcatga gctcttgcag gttattaagg agaaaacgga ggtaatagaa gtatgattta
    12541 tacagtcaca ctcaatccat ccattgacta tatcgttcgt ttggaccaag tcaaagttgg
    12601 tagtgtaaat cgtatggaca gtgatgataa gtttgctggt gggaaaggaa tcaatgtcag
    12661 ccgtgtcttg aaacgtttga atataccaaa tacagcgacg ggatttatcg gtggctttac
    12721 tggtaaattt atcacagata ctttagcaga ggaagaaatc gagacacgtt ttgtccaggt
    12781 ggcagaagat actcgtatca atgttaaaat caaagcagac caagaaacag aaatcaacgg
    12841 aacgggtcca actgttgaac cggttcagct agaagaattg aaagctattt tatctagtct
    12901 gacagcagaa gatacagttg tctttgcagg ttcaagtgct aaaaatctag gcaatgttat
    12961 ctataaggat ttgatttcct tgacgcgcca gactggtgcg caagtggtct gtgactttga
    13021 aggacagacc ttaattgata gtttggacta ccagcctctt cttgtaaaac caaacaatca
    13081 tgaacttgga gcgatttttg gggttaaact cgaaagttta gatgaaattg agaaatacgc
    13141 tcgtgagtta ctggctaagg gtgctcaaaa tgttattatc tctatggctg gtgatggcgc
    13201 ccttcttgtc acatctgagg gagcttactt cgctaaacca atcaaaggaa cagtcaaaaa
    13261 ttcagttgga gctggtgatt ctatggttgc tggattcaca ggtgaatttg tcaaatcaaa
    13321 agacgcagta gaagccttca aatggggagt ggcttgcgga acggcaacta ccttctcaga
    13381 tgacttggca acggcggaat ttattaaaga aacatatgga aaagttgagg tagaaaaaag
    13441 atgaaaattc aagacctatt gagaaaagat gtcatgttgc tagatttgca ggcaactgaa
    13501 aaaacagctg tcatcgacga gatgattaaa aatttgacag agcacggtta tgtaacagat
    13561 tttgaaacat ttaaagaagg aattttggcg cgtgaagctt tgacttctac tggtttgggt
    13621 gatggaatcg caatgcctca cagcaaaaac gctgctgtca aagaagcgac agttctattt
    13681 gctaagtcaa ataagggtgt tgactacgag agcttggatg gacaagcaac tgacctcttc
    13741 ttcatgattg cagctccaga aggtgccaat gatactcact tggcagcctt ggcagaattg
    13801 tctcaatact tgatgaaaga cggttttgca gacaaacttc gtcaagcaac atctgcagac
    13861 caagttatcg aactttttga ccaagcttca gaaaaaactg aggaacttgt tcaagcacct
    13921 gctaatgact ctggtgactt tatcgtagct gttacagctt gtacaacagg tattgcccac
    13981 acttacatgg cccaagaagc ccttcaaaaa gtagctgctg aaatgggggt tggtatcaag
    14041 gtcgaaacca acggtgctag cggtgttgga aatcaactaa ctgcagaaga tatccgtaag
    14101 gctaaagcta ttatcattgc agcagacaag gccgttgaaa tggatcgatt tgatggaaaa
    14161 ccattgatca atcgtccagt tgctgacggt atccgtaaga cagaagagct aattaacttg
    14221 gctctttcag gagatactga agtctaccgt gccgctaatg gtgccaaagc tgcaacagcc
    14281 tctaacgaaa aacaaagcct tggtggtgcc ttgtacaaac acttgatgag tggtgtatct
    14341 caaatgttac cattcgttat cggtggtggt atcatgattg cccttgcctt cttgattgac
    14401 ggtgctttgg gtgttccaaa tgaaaacctt ggcaatcttg gttcttacca tgagttagct
    14461 tctatgttca tgaaaattgg tggagctgcc tttggtttga tgcttccagt ctttgcgggt
    14521 tatgttgcct actctattgc tgaaaaaccg ggtttggtag caggtttcgt ggctggtgct
    14581 attgccaaag aaggttttgc ctttggtaaa attccttatg ccgcaggtgg tgaagcaact
    14641 tcaactcttg caggtgtctc atctggtttc ctaggtgccc ttgttggtgg atttatcgca
    14701 ggtgccttgg ttcttgccat caagaaatac gttaaagttc ctcgttcact cgaaggtgct
    14761 aaatcaatcc ttctattgcc acttcttgga acaatcttga caggatttgt tatgctagct
    14821 gtgaatatcc caatggctgc aatcaacact gctatgaatg acttcctagg cggtcttgga
    14881 ggaggttcag ctgtccttct tggtatcgtc cttggtggaa tgatggctgt tgacatgggt
    14941 ggaccagtta ataaagcagc ttatgtcttt ggtacaggta cgcttgcagc aactgtttct
    15001 tcaggtggtt ctgtagccat ggcagcagtt atggctggag gaatggtgcc accacttgca
    15061 atctttgtcg caactcttct tttcaaagat aaatttacta aggaagaacg taactctggt
    15121 ttgacaaaca tcatcatggg cttgtcattt atcactgagg gagcgattcc atttggtgcc
    15181 gctgacccag ctcgtgcgat tccaagcttc atccttggtt cagcagtagc aggtggactc
    15241 gttggtctta ctggtatcaa actcatggcg ccacacggag gaatcttcgt tatcgccctt
    15301 acttcaaatg ctctccttta cctcgtttct gtcttggtag gagcaatcgt aagtggtgtg
    15361 gtttatggtt acctacgcaa accacaagca taaaaaatag aaaaatgaaa agattggacc
    15421 gtttggtgca gtctttttct cttcccgaaa tgcctgtgaa atatggtata atagaagaat
    15481 ggcaaacaag aatacaagta caacaagacg gagaccgtct aaagcagaac tggaaagaaa
    15541 agaagcgatt caacgaatgt tgatttcgtt aggaattgcg attttattga ttttcgcagc
    15601 cttcaaatta ggggctgcag gtataaccct ttataattta attcgcttgc tagtgggtag
    15661 cctagcttat ctggcgatat tcggcctatt aatctatctc ttctttttca agtggatacg
    15721 aaaacaggaa ggactcttat ctggcttttt caccatattt gctggcttac tcttgatttt
    15781 tgaggcctac ttggtttgga aatatggttt ggacaagtcc gttctaaaag ggaccatggc
    15841 tcaggttgtg acagatctga ctggttttcg aacgactagc tttgctggag ggggcttgat
    15901 cggggtcgct ctttatattc caacagcctt tctcttttca aatatcggaa cttactttat
    15961 tggttctatc ttgattttag tgggttctct cctagtcagc ccttggtctg tttacgatat
    16021 tgctgaattt ttcagtagag gctttgccaa atggtgggaa gggcacgagc gtcgaaaaga
    16081 ggaacgcttt gtcaaacaag aagaaaaagc tcgccaaaag gctgagaaag aggctagatt
    16141 agaacaagaa gagactgaaa aagccttact cgatttgcct cctgttgata tggaaacggg
    16201 tgaaattctg acagaggaag ctgttcaaaa tcttccacct attccagaag aaaagtgggt
    16261 ggaaccagaa atcatcctgc ctcaagctga acttaaattc cctgaacagg aagatgactc
    16321 agatgacgaa gatgttcagg tcgatttttc agccaaagaa gcccttgaat acaaacttcc
    16381 aagcttacaa ctctttgcac cagataaacc aaaagatcag tctaaagaga agaaaattgt
    16441 cagagaaaat atcaaaatct tagaagcaac ctttgctagc tttggtatta aggtaacagt
    16501 tgaacgggcc gaaattgggc catcagtgac caagtatgaa gtcaagccgg ctgttggtgt
    16561 aagggtcaac cgcatttcca atctatcaga tgacctcgct ctagccttgg ctgccaaaga
    16621 tgtccggatt gaagcaccaa tccctgggaa atccctaatc ggaattgaag tgcccaactc
    16681 cgatattgcc actgtatctt tccgagaact atgggaacaa tcgcaaacga aagcagaaaa
    16741 tttcttggaa attcctttag ggaaggctgt taatggaacc gcaagagctt ttgacctttc
    16801 taaaatgccc cacttgctag ttgcaggttc aacgggttca gggaagtcag tagcagttaa
    16861 cggcattatt gctagcattc tcatgaaggc gagaccagat caagttaaat ttatgatggt
    16921 cgatcccaag atggttgagt tatctgttta caatgatatt ccccacctct tgattccagt
    16981 cgtgaccaat ccacgcaaag ccagcaaggc tctgcaaaag gttgtggatg aaatggaaaa
    17041 ccgttatgaa ctctttgcca aggtgggagt tcggaatatt gcaggtttta atgccaaggt
    17101 agaagagttc aattcccagt ctgagtacaa gcaaattccg ctaccattca ttgtcgtgat
    17161 tgtggatgag ttggctgacc tcatgatggt ggccagcaag gaagtggaag atgctatcat
    17221 ccgtcttggg cagaaggcgc gtgctgcagg tatccacatg attcttgcaa ctcagcgtcc
    17281 atctgttgat gtcatctctg gtttgattaa ggccaatgtt ccatctcgtg tagcatttgc
    17341 ggtttcatca ggaacagact cccgtacgat tttggatgaa aatggagcag aaaaacttct
    17401 tggtcgagga gacatgctct ttaaaccgat tgatgaaaat catccagttc gtctccaagg
    17461 ctcctttatc tcggatgacg atgttgagcg cattgtgaac ttcatcaagg ctcaggcaga
    17521 tgcagactac gatgagagtt ttgatccagg tgaggtttct gaaaatgaag gagaattttc
    17581 ggatggagat gctggtggtg atccgctttt tgaagaagct aagtctttgg ttatcgaaac
    17641 acagaaagcc agtgcgtcta tgattcagcg tcgtttatca gttggattta accgtgcgac
    17701 ccgtctcatg gaagaactgg agatagcagg tgtcatcggt ccagctgaag gtaccaaacc
    17761 tcgaaaagtg ttacaacaat aaaaaaatag cttctttccg agtttggagg gaagctattt
    17821 tagtggctat tgattacttt tatactcttc gcaaatctct tcaaaccacg tcagcttcgc
    17881 cttgccgtac tcaagtactg tctacggcta gcttcctagt ttgctctttg attttcattg
    17941 agtattattt tctgaagttg gagtattggg ctgtttttca ttttcagtgg caggtttact
    18001 tgtagcaggt gtagtagagt cctgagttgc tgttttctga tcttcttttt tctcttcctt
    18061 gacgctagat tttggcgttt cttcttgttg tgttttttct tgagaagtgt tattttcctt
    18121 atttggagta gtattttcct gaggggattc tttttgaatt tctttgacaa aggttgtcgt
    18181 ctggcttgtc gtaggttctt ttttagtatt tttattatta ttatccaagg cgttcatgat
    18241 cgtgataccc gcggtaatca aaccaaggat actaccgata gtagtaatgg ttttttgaaa
    18301 aacagtaaag ccttttttga tatgttctgt tttcggtttt gaagttctac gatagttaga
    18361 catacattct ctcctttgat tatgatttat tatactctgt ttctttaaaa aaatcttaaa
    18421 aaaagagaaa ttgccctttc ttgtcgcagg ctccgtttca tgatacaata gaaggagtga
    18481 ttttagaaag cgtgagaaac atgatttact ttgataattc ggcgacgacc aggccctatc
    18541 cagaagccct tgaaacctat atgcaggtgg cttcaaaaat tttaggaaat ccatctagtc
    18601 tccatcgttt gggagaccag gcaacacgaa ttttagatgc ttcccgtcaa cagattgcag
    18661 atttaatcgg taagaaaagt gatgaaatct tctttacatc tggtgggaca gaaggggata
    18721 actggatcat caagggagta gcatttgaaa aagcccagtt tggcaagcac atcatcgtat
    18781 cagccattga acatccagca gttaaggaat cagccctctg gttgaaagct caaggttttg
    18841 aagtggattt tgctccagtt gataacaaag gatttgtgga tgttgaggcg ctagaagatt
    18901 tgataagacc tgatacgacc ctcgtttcca tcatggctgt gaacaatgaa atcggctcta
    18961 tccaacctat tgaggctatt tcagaattct tggcagacaa gccaactatt tccttccacg
    19021 ttgatgcggt tcaggcgctt gccaagattc cgactgaaaa gtatctgaca gaacgagtgg
    19081 atttcgcgac tttctctggt cacaagttcc acggaatccg tggtgttggt tttatctata
    19141 tcaagtctgg caagaagatt acgcctcttt taacaggtgg tggtcaggaa cgtgattacc
    19201 gttcgacaac tgaaaatgtg gcaggaattg cagcgaccgc taaggctctc cgtttatcta
    19261 tggaaaagct agatatcttt aggagcaaga ctgggcagat gaaggcagtg attcgtcaag
    19321 cacttctgga ctatccggat atatttgtct tttcagatga ggaagacttc gcccctcata
    19381 ttctgacttt tggaatcaag ggtgttcgtg gtgaggtcat tgttcacgcc tttgaagact
    19441 atgatatttt catctcaacg acctcggctt gttcgtccaa ggctgggaaa ccagccggaa
    19501 ccctgattgc tatgggagtg gacaaggata aggctcagtc agctgtgcgt cttagtctag
    19561 accttgaaaa tgatatgagt caggtcgagc agttcttgac caagctaaaa ttaatttaca
    19621 atcaaactag aaaagtaaga taggagcatt catgcagtat tcagaaatta tgattcgcta
    19681 cggtgagctg tcaaccaagg gcaaaaatcg tatgcgtttc atcaataaac ttcgcaataa
    19741 tatttcagac gttttgtcta tctatcccca agttaaggta acagcagatc gcgaccgtgc
    19801 ccacgcttac ctcaatggag cagattacac agcagtagca gaatcgctca aacaagtatt
    19861 tggaattcaa aacttttccc ctgtttataa ggttgaaaaa tctgtagaag ttctgaagtc
    19921 tgctgtccaa gagattatgc aggacatcta caaggaaggc atgaccttta aaatctctag
    19981 caagcgtagc gaccacacct ttgaattgga cagtcgtgaa ctcaaccaaa cgcttggagg
    20041 ggctgtattc gaagccattc caaacgtaca agctcaaatg aaaagtcctg acatcaatct
    20101 tcaggtggag atacgtgaag aagcagccta tctttcttat gaaaccgttc gaggggcagg
    20161 tggattacca gtgggaactt ctggtaaggg catgctcatg ttgtcagggg gaatcgattc
    20221 ccctgtagct ggctatctag cgcttaaacg aggggtagat atagaggcag ttcactttgc
    20281 cagcccacct tacacgagtc ctggtgctct taaaaaagcc caagatttga cccgtaaatt
    20341 aaccaaattt gggggaaata tccagtttat cgaagttcct tttacagaga ttcaagagga
    20401 aatcaaagcc aaagcgccag aagcctacct tatgaccttg acgcgtcgtt ttatgatgcg
    20461 tattacggac cgtattcgtg aggtacgaaa tggtttagtt atcatcaatg gggaaagtct
    20521 aggtcaagta gccagccaaa cccttgaaag tatgaaggct atcaatgctg ttaccaacac
    20581 tcccatcatt cgtcctgtgg ttaccatgga caagttggaa atcattgaca tcgcccagga
    20641 aatcgatacc tttgacattt caatccaacc gtttgaagac tgttgtacta tttttgcacc
    20701 agatcgtcca aaaacaaatc ctaaaattaa gaatgcggag cagtacgaag cgcgtatgga
    20761 tgttgaaggc ttggttgagc gagcagtggc tggaatcatg attactgaaa tcacacctca
    20821 agccgaaaaa gatgaagttg atgacttgat tgacaatctg ctctaattca gaaaatccaa
    20881 aagaatagcg aaaatcagta aaaaaagtta gttttttctc taaaaacagg taaaaaacta
    20941 acttttttta tttttatgat ataatgatat aaaattttga atatagagag ttttctgaca
    21001 atgaatcaat cctactttta tctaaaaatg aaagaacaca aactcaaggt tccttataca
    21061 ggtaaggagc gccgtgtacg tattcttctt cctaaagatt atgagaaaga tacagaccgt
    21121 tcctatcctg ttgtatactt tcatgacggg caaaatgttt ttaatagcaa agagtctttc
    21181 attggacatt catggaagat tatcccagct atcaaacgaa atccggatat cagtcgcatg
    21241 attgtcgttg ctattgacaa tgatggtatg gggcggatga atgagtatgc ggcttggaag
    21301 ttccaagaat ctcctatccc agagcagcag tttggtggta agggtgtgga gtatgctgag
    21361 tttgtcatgg aggtggtcaa gccttttatc aagcacaaaa caggttggtt tgatggcatg
    21421 atgacgacag gttgctctat gggagcctat catgcactca atttcttcct ccagcatcca
    21481 gatgtcttta ccaaagtgat tgctctcagt ggtgtttacg acgcacgttt ctttgtcggc
    21541 gattactaca acgatgatgc tatttaccaa aactcgccag tagattatat ttggaaccaa
    21601 aacgatggct ggtttattga ccgttaccgt caggcagaga ttgtgctgtg tacggggctt
    21661 ggagcctggg aacaagatgg tttaccatcc ttttacaagc tcaaagaagc ctttgaccag
    21721 aaacaaattc cagcctggtt tgctgaatgg ggacatgatg tcgcccacga ctgggaatgg
    21781 tggcgtaaac aaatgcttta tttcctcggt aatctctatt tataaaagga gttacctatg
    21841 aattaccttg ttatttctcc ctactatcca caaaactttc aacagtttac catcgaacta
    21901 gctaataaag gcatcacagt cttgggaatt ggtcaagagt cttacgagca attggatgag
    21961 cccttgcgca atagcttgac cgagtatttt cgtgttgata atcttgagaa catagatgaa
    22021 gtcaaacgtg cagttgcttt tctcttttat aaacatggtc caattgaccg catcgagtct
    22081 cacaatgaat actggcttga gctagacgca acactcagag aacaattcaa tgtttttggt
    22141 gccaaaccag aggatctcaa aaagacgaaa tataagtctg aaatgaagaa acttttcaaa
    22201 aaagcaggtg ttcctgtggt acctggagct gttatcaaga cggaagcaga tgttgatcaa
    22261 gcagtgaaag aaatcggtct tccaatgatt gccaaacctg ataatggagt gggagcagcc
    22321 gcaaccttta aacttgagac agaagacgat atcaatcact tcaagcaaga atgggaccat
    22381 tcaacccttt atttctttga aaaatttgtc acttccagcg aaatctgtac ctttgacggg
    22441 ctcgtggaca aggatggaaa gattgtcttc tcaacaacct ttgactacgc ctatacaccg
    22501 cttgacctca tgatttataa gatggacaat tcttattatg tgctcaagga tatggatcct
    22561 aaactgcgca agtatgggga agcaattgtc aaagaatttg gtatgaaaga acggtttttc
    22621 catattgagt tcttccgtga gggggacgat tatattacca tcgagtacaa taaccgccct
    22681 gcaggtggtt ttaccattga tgtttataac tttgctcatt ccttggacct ttatcgtggc
    22741 tatgcagcta ttgtcgcagg agaggagttc ccggcgtcag actttgaaac tcagtattgt
    22801 ttggctactt ctcgccgtgc aaatgctcac tatgtttatt cagaagagga tttgcttgcc
    22861 aaatatagcc agcagttcaa ggttaaaaaa gtcatgccag ctgccttcgc ggaacttcaa
    22921 ggagattacc tgtatatgct gacaactccg agtcgacaag aaatggatca gatgattgct
    22981 gatttcggac aacgtcaaga ataagaacta tcggattaag gaaattcact cccttaatcc
    23041 ttttgttttg tctggtaaaa aataagagca tcctaacaag gtagctatca taaaacttgt
    23101 tcgataacta tttgaagcag gattaggtgg ctagaaattg aattttaaca tttcaattta
    23161 gtcatagtat tgtgttttcg tatccttaaa tcagctaaaa ggatccatga cgacacctat
    23221 acgatatagt tttcaggata ccaaaccagt ctattaatac tcaatgaaaa tcaaagagca
    23281 aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtgg atagaact
//
LOCUS       DAVMMR010000033        21332 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMMR010000033 DAVMMR010000000
VERSION     DAVMMR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.033"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..425
                     /locus_tag="VYS74_001244"
     CDS             <1..425
                     /locus_tag="VYS74_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV3789288.1"
                     /translation="EPSEQDFDLQVFQSALAKSKKPIKSHLLDQTLVAGLGNIYVDEV
                     LWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGSTIRTYTNAFGEDGSMQDF
                     HQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            425..1030
                     /locus_tag="VYS74_001245"
     CDS             425..1030
                     /locus_tag="VYS74_001245"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV3789289.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFEQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            1017..2216
                     /locus_tag="VYS74_001246"
     CDS             1017..2216
                     /locus_tag="VYS74_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV3789290.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            2216..2365
                     /gene="rpmG"
                     /locus_tag="VYS74_001247"
     CDS             2216..2365
                     /gene="rpmG"
                     /locus_tag="VYS74_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3789291.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKEKKVTL
                     HLESK"
     gene            2405..2638
                     /gene="secG"
                     /locus_tag="VYS74_001248"
     CDS             2405..2638
                     /gene="secG"
                     /locus_tag="VYS74_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV3789292.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            2740..5094
                     /gene="rnr"
                     /locus_tag="VYS74_001249"
     CDS             2740..5094
                     /gene="rnr"
                     /locus_tag="VYS74_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV3789293.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIVEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGIGRGSNRRSDKKEDKRKS
                     GRSNDKRKYSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            5057..5524
                     /gene="smpB"
                     /locus_tag="VYS74_001250"
     CDS             5057..5524
                     /gene="smpB"
                     /locus_tag="VYS74_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV3789294.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            5540..6400
                     /gene="tehB"
                     /locus_tag="VYS74_001251"
     CDS             5540..6400
                     /gene="tehB"
                     /locus_tag="VYS74_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV3789295.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            6473..7426
                     /gene="coiA"
                     /locus_tag="VYS74_001252"
     CDS             6473..7426
                     /gene="coiA"
                     /locus_tag="VYS74_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV3789296.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            7445..9247
                     /gene="pepF"
                     /locus_tag="VYS74_001253"
     CDS             7445..9247
                     /gene="pepF"
                     /locus_tag="VYS74_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3789297.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            9249..9962
                     /locus_tag="VYS74_001254"
     CDS             9249..9962
                     /locus_tag="VYS74_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV3789298.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            10029..10970
                     /gene="prsA"
                     /locus_tag="VYS74_001255"
     CDS             10029..10970
                     /gene="prsA"
                     /locus_tag="VYS74_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV3789299.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(11087..11428)
                     /locus_tag="VYS74_001256"
                     /pseudo
     CDS             complement(11087..11428)
                     /locus_tag="VYS74_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11842..11937
                     /locus_tag="VYS74_001257"
                     /pseudo
     CDS             11842..11937
                     /locus_tag="VYS74_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12191..12811)
                     /locus_tag="VYS74_001258"
     CDS             complement(12191..12811)
                     /locus_tag="VYS74_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3789300.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(12811..13293)
                     /locus_tag="VYS74_001259"
     CDS             complement(12811..13293)
                     /locus_tag="VYS74_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV3789301.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(13293..14135)
                     /locus_tag="VYS74_001260"
     CDS             complement(13293..14135)
                     /locus_tag="VYS74_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV3789302.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGALAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLTWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(14230..15030)
                     /locus_tag="VYS74_001261"
     CDS             complement(14230..15030)
                     /locus_tag="VYS74_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV3789303.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            15174..16553
                     /gene="glmU"
                     /locus_tag="VYS74_001262"
     CDS             15174..16553
                     /gene="glmU"
                     /locus_tag="VYS74_001262"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV3789304.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            16563..17108
                     /locus_tag="VYS74_001263"
     CDS             16563..17108
                     /locus_tag="VYS74_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3789305.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            17120..17434
                     /locus_tag="VYS74_001264"
     CDS             17120..17434
                     /locus_tag="VYS74_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV3789306.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKNRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            17451..18143
                     /locus_tag="VYS74_001265"
     CDS             17451..18143
                     /locus_tag="VYS74_001265"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV3789307.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            18220..18711
                     /gene="rocS"
                     /locus_tag="VYS74_001266"
     CDS             18220..18711
                     /gene="rocS"
                     /locus_tag="VYS74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV3789308.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            18809..19399
                     /locus_tag="VYS74_001267"
     CDS             18809..19399
                     /locus_tag="VYS74_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV3789309.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            19408..21171
                     /locus_tag="VYS74_001268"
     CDS             19408..21171
                     /locus_tag="VYS74_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV3789310.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         6580 a         3878 c         4609 g         6265 t
ORIGIN      
        1 ctgaaccaag cgaacaagac tttgatttac aggtctttca atctgccctt gccaagtcca
       61 aaaagcctat caaatcccat ctcctagacc agaccttggt agctggactt ggcaatattt
      121 atgtggatga ggttctctgg cgagctcagg ttcatccagc tagatcttcc cagactttga
      181 cagcagaaga agcgacagct attcatgacc aaaccattgc tgttttgagc caggctgttg
      241 aaaaaggcgg ttccaccatt cggacttata ccaatgcctt tggggaagat ggaagcatgc
      301 aggactttca tcaggtctat gataagactg gtcaagagtg tgtacgctgt ggtaccatca
      361 ttgagaaaat tcaactaggc ggacgaggca cccacttttg tccaaactgt caaaggaggg
      421 actgatgaga aaaatcatcg gaatcactgg gggaattgcc tctggtaagt caactgtgac
      481 aaattttcta agacagcaag gctttcaagt agtggatgcc gacgcagtcg tccaccaact
      541 acagaaacct ggtggtcgtc tgtttgaggc tctagtacag cactttgagc aagaaatcat
      601 tcttgaaaac ggagaactca atcgccctct cctagctagt ctcatctttt caaatcctga
      661 agagcaaaaa tggtcgaagc aaattcaagg ggagattatc cgtgaggaac tggctacttt
      721 gagagaacag ttggctcaga cagaagagat tttcttcatg gatattcccc tactttttga
      781 gcaggactac agcgattggt ttgctgagac ttggttggtc tatgtggacc gagatgccca
      841 agtagaacgc ttaatgaaaa gggaccagtt gtccaaagat gaagctgagt ctcgtctggc
      901 agcccagtgg cctttagaaa aaaagaaaga tttggccagc caggttcttg ataataatgg
      961 caatcagaac cagcttctta atcaagtgca tatccttctt gagggaggta ggcaagatga
     1021 cagagattaa ctggaaggat aatctgcgta ttgcctggtt tggtaatttt ctgacaggag
     1081 ccagtatttc tttggttgta ccttttatgc ccatcttcgt ggaaaatcta ggtgtaggga
     1141 gtcagcaagt cgctttttat gcaggcttag caatttctgt ctctgctatt tccgcggcgc
     1201 tcttttctcc tatttggggt attcttgctg acaaatacgg ccgaaaaccc atgatgattc
     1261 gggcaggtct tgctatgact atcactatgg gaggcttggc ctttgtccca aatatctatt
     1321 ggttaatctt tcttcgttta ctaaacggtg tatttgcagg ttttgttcct aatgcaacgg
     1381 cactgatagc cagtcaggtt ccaaaggaga aatcaggctc tgccttaggt actttgtcta
     1441 caggcgtagt tgcaggtact ctaactggtc cctttattgg tggctttatc gcagaattat
     1501 ttggcattcg tacagttttc ttactggttg gtagttttct atttttagct gctattttga
     1561 ctatttgctt tatcaaggaa gattttcaac cagtagccaa ggaaaaggct attccaacaa
     1621 aggaattatt tacctcggtt aaatatccct atcttttgct caatctcttt ttaaccagtt
     1681 ttgtcataca attttcagcc caatcgatcg gccctatttt ggctctttat gtacgcgact
     1741 tagggcagac agagaatctt ctttttgtct ctggtttgat tgtgtccagt atgggctttt
     1801 ccagcatgat gagtgcagga gtcatgggca agctaggtga caaggtgggc aatcatcgtc
     1861 tcttggttgt cgcccagttt tattcagtca tcatctatct cctctgtgcc aatgcctcta
     1921 gcccccttca actaggactc tatcgtttcc tctttggatt gggaaccggt gccttgattc
     1981 ccggggttaa tgccctactc agcaaaatga ctcccaaagc cggcatttcg agggtctttg
     2041 ccttcaatca ggtattcttt tatctgggag gtgttgttgg tcccatggca ggttctgcag
     2101 tagcaggtca atttggctac catgctgtct tttatgcgac gagcctttgt gttgccttta
     2161 gttgtctctt taacctgatt caatttcgaa cattattaaa agtaaaggaa atctagtgcg
     2221 agtaaaaatc aatctcaaat gctcctcttg tggcagtatc aattacctaa ccagtaaaaa
     2281 ttcaaaaacc catccagaca agattgaggt tttaaagtat tgtcccaagg aaaaaaaagt
     2341 aaccctacat cttgaatcta agtagatttt atggtaaaat aatagggatt ttaaggagtt
     2401 tgatatgtat aacctattat taaccatttt attagtatta tctgttgtga ttgtgattgc
     2461 aattttcatg caaccaacca aaaaccaatc cagcaatgta tttgatgcca gttcaggtga
     2521 tttgtttgaa cgcagtaaag ctcgcggttt tgaagctgta atgcagcgtt tgacagggat
     2581 tttagtcttt ttctggctag ccattgcctt agcattgacg gtattatcaa gtagataaga
     2641 aaataatggg caggactagg tctttgcctc tttttatttt taaaggatgt ttgagaaggt
     2701 tttacagtaa aagaaaatta aaaaatctag aaagaaaata tgaaagatag aataaaagaa
     2761 tatttacaag acaagggaaa ggtgactgtt aatgatttgg ctcaggcttt gggaaaagac
     2821 agttccaagg attttcgtga gttgattaaa accttgtcct taatggaaag aaagcaccaa
     2881 attcgttttg aagaagatgg tagtctgaca ttagaaatta agaaaaaaca tgagattacc
     2941 ctcaagggga tttttcatgc ccataaaaat ggctttggct ttgttagtct ggaaggcgag
     3001 gaggacgacc tttttgtagg gaaaaatgat gtcaactatg ctattgatgg tgataccgtc
     3061 gaggtagtga ttaagaaagt cgctgaccgc aataagggaa cagcagcaga agccaaaatt
     3121 attgatatcc tagaacacag tttgacaaca gttgtcgggc aaatcgttct ggatcaggaa
     3181 aaacctaagt atgctggcta tattcgttca aaaaatcaga aaatcagtca accgatttat
     3241 gttaagaaac cagccctaaa attagaagga acagaagttc tcaaggtctt tatcgataaa
     3301 tacccaagca agaaacatga tttctttgtc gcgagtgttc tcgatgtggt gggacactca
     3361 acggatgtcg gaattgatgt tcttgaggtt ttggaatcaa tggacattgt atccgagttt
     3421 ccagaagctg ttgttaagga agcagaaagt gtgcctgatg ctccgtctca aaaggatatg
     3481 gaaggtcgtc tggatctaag agatgaaatt acctttacca ttgacggtgc ggatgccaag
     3541 gacttggacg atgcagtgca tatcaaggct ctgaaaaatg gcaatctgga gcttggggtt
     3601 cacatcgcag atgtttctta ttatgtgacc gaggggtctg cccttgacaa ggaagccctt
     3661 aaccgtgcga cttctgttta cgtgacagac cgagtggtgc caatgcttcc agaacgacta
     3721 tcaaatggca tctgctctct caatccccaa gttgaccgcc tgacccagtc tgctattatg
     3781 gagattgata aacatggtcg tgtggtcaac tataccatta cacaaacagt tatcaagacc
     3841 agttttcgta tgacctatag cgatgtcaat gatatcctag ctggcgatga agaaaagaga
     3901 aaagaatatc ataaaattgt accaagtatc gaactcatgg ccaagcttca tgaaacttta
     3961 gaaaacatgc gtgtgaaacg tggagctctc aattttgata ccaatgaagc gaagatttta
     4021 gtggataaac aaggtaagcc tgttgatatc gttcttcggc agcgtggcat tgccgagcgg
     4081 atgattgagt cttttatgtt gatggctaat gaaacagttg ccgaacattt cagcaagttg
     4141 gatttgcctt ttatctatcg aattcacgag gagcctaagg ctgaaaaggt tcagaagttt
     4201 attgattatg cttcgagttt tggcttgcgc atttatggaa ctgccagtga gattagtcag
     4261 gaggcacttc aagacatcat gcgtgctgtt gagggagaac cttatgcaga tgtattgtcc
     4321 atgatgcttc ttcgctctat gcagcaggct cgttattcgg agcacaatca cggccactat
     4381 ggactagctg ctgactatta tactcacttt accagtccaa ttcgtcgtta tccagacctt
     4441 cttgttcacc gtatgattcg ggattacggc cgttctaagg aaatagtaga gcattttgag
     4501 caagtgattc cagagattgc gacccagtct tccaaccgtg aacgtcgtgc catagaagct
     4561 gagcgtgaag tcgaagccat gaaaaaggct gagtatatgg aagaatacgt gggtgaagag
     4621 tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct ttgtcgaatt gccaaacaca
     4681 gttgaaggct tgattcacat cactaatcta cctgaatttt atcatttcaa tgagcgtgat
     4741 ttgactcttc gtggagaaaa atcaggtatc actttccgag tgggtcagca gatccgtatc
     4801 cgtgttgaaa gagcggataa aatgactgga gagattgatt tttcattcgt acctagtgag
     4861 tttgatgtga ttgaaaaagg cttgaaacag tctagtcgta gtggcatagg gcgtggttcc
     4921 aatcgtcgtt cggataagaa ggaagacaag agaaaatcag gacgctcaaa tgataagcgt
     4981 aagtattcac aaaaagacaa gaagaaaaaa ggaaagaaac ctttttacaa ggaagtagct
     5041 aagaaaggag ccaagcatgg caaagggcga gggaaaggtc gtcgcacaaa ataaaaaggc
     5101 acgccacgac tatacaatcg tagatacgct agaggcaggg atggtcctga ctggaactga
     5161 aatcaagagt gtacgagctg ctcgaattaa tctcaaggat ggctttgctc aagtgaaaaa
     5221 tggagaagtt tggctgagca atgttcatat cgcgccttac gaagagggca atatctggaa
     5281 ccaggaacca gaacgtcgtc gtaaactcct gctccataaa aagcaaattc aaaaattgga
     5341 acaagagacc aaagggacag gaatgacctt agttcccctt aaggtctata taaaagatgg
     5401 ctacgctaag cttcttttag gacttgccaa agggaagcat gactatgaca aacgggagtc
     5461 tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg atgaaagctg ttaatcagcg
     5521 ataaaaagag gaattgaaaa tggaaaaatt agttgcctat aaacgcatgc ctttgtggaa
     5581 taaacaaaca atgcctgaag ctgttcagca aaagcacaat acaaaagttg ggacttgggg
     5641 gaaaattact gtcttgaagg gagctctcaa gtttattgaa ttgacagaag aaggggaagt
     5701 tctagctgaa cacctctttg aagcaggggc agacaatcca atggcccaac ctcaagcctg
     5761 gcaccgagtg gaagctgcca cagatgatgt ggaatggtac ttggaatttt attgtaaacc
     5821 tgaggattat tttgctaaaa aatacaatac caatcctgtt cattcagagg tcctagaggc
     5881 catgcagaca gtgaaacaag ggaaagcttt ggatttgggt tgtggtcagg ggcgtaattc
     5941 tctttttcta gcccagcaag attttgatgt gacggctgta gatcaaaatg gactagctct
     6001 tgaaatcttg caaagcattg tggagcagga agatttggac atgcctgttg gcctttacga
     6061 tatcaattca gctagcattg aacaagaata tgattttatc gtttcaacag ttgttctcat
     6121 gtttctacaa gcggaccgca ttccagctat tattcaaaat atgcaggaga aaaccagtgt
     6181 tggtggttac aaccttatcg tttgtgccat ggacacggag gattatcctt gctcggttaa
     6241 cttcccattc acctttaaag aaggagaact ggcagactat tacaaggatt gggaattggt
     6301 taagtacaat gaaaatccag gccatttgca ccgtcgcgat gagaatggca atcgtattca
     6361 actacgcttt gcgaccttac tagctaagaa aatcaagtaa acatacatga agattaggaa
     6421 ttttcctgat cttttttctt ttttacgaat gatatagaaa aggagggaat tcatgtttgt
     6481 tgcgagagat gctaggggag aattggtaaa tgtgttagag gataaacttg agaagcaagc
     6541 atacacctgc ccagcttgtg gaggccagat ccatttgcgt caaggaccaa gtgtacggac
     6601 gcattttgcc cataaatcct taaaagactg tgattttttc tttgaaaatg aaagtccaga
     6661 acacctggcc aataaggaat ccctctatca ctggttgaaa aaagagacaa aggttcaatt
     6721 agagtacccg ctttcagaac ttaaacagat tgcggatgta tttgtaaatg gcaatctagc
     6781 tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt aaagagcgaa gtgagggcta
     6841 tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa aaactgtggc tcaaggagcg
     6901 tttgactcgt ctacagcaag gttttcttta tttcagtcaa aacatgggct tttatgtttg
     6961 ggaattagac aaggaaaaac aagttttaag actcaaatac ctgatttacc aggatctccg
     7021 cggtaaactc cattatcaaa tcaaggaatt ttcctatggt caaggtagtt tattggaaat
     7081 attgcgtctt ccctataaga gacaaaaaat atctcatttt acagtttctg aggacaagga
     7141 catctgtcgc tatatccggc aacaacttta ttatcaaaat ctcttttgga tgaaagaaca
     7201 agcagaagcc tatcaaaagg gagaaaatat cctgacttat ggactgaaag aatggtatcc
     7261 acaaattcga ccaatagtgg gcaaattttt ccagattgaa caagacttga ctagctatta
     7321 tcagcacttt tatacctatt accaaaaaaa tcctcaaaat gattggcaaa agctttatcc
     7381 accagccttt tatcagcaat atttcttgaa aaatatggta gaatagaaag gatggaggaa
     7441 tctaatggta ttacaaagaa atgaaataaa tgaaaaagat acatgggatc tatcaacgat
     7501 ctacccaact gaccaggctt gggaagaagc cttaaaagat ttaacagaac aattggagac
     7561 agtagcccag tatgaaggcc atctcttgga tagtgcggat aacctactag aaatcactga
     7621 attttctctt gaaatggaac gccagataga gaagctttac gtttatgctc atatgaagaa
     7681 tgaccaggat acacgtgaag ctaagtatca agagtactat gccaaggcca tgacactcta
     7741 cagccagtta gaccaagcct tttcattcta tgagcctgaa tttatggaga ttagcgaaaa
     7801 gcagtatgct gactttttag aagctcaacc aaagctgcag gtttatcaac actattttga
     7861 caagctcttg caaggcaagg atcacgttct ttcacaacgt gaagaagaat tattggctgg
     7921 agctggagaa atctttggtt cagcaagtga aaccttcgct atcttggaca atgcggatat
     7981 tgtgttccct tatgtcctag acgatgatgg taaagaagtt cagctatctc atgggactta
     8041 cacacgtttg atggagtcta aaaaacgtga ggttcgccgt ggtgcctatc aagctcttta
     8101 tgcgacttac gaacaattcc aacacaccta tgccaaaacc ttgcaaacca atgttaaggt
     8161 gcaaaattac cgtgctaaag ttcgtaacta caagagtgct cgtcatgcag ccctcgcagc
     8221 gaattttgtt ccagaaagtg tttatgacaa tttggtagca gcagttcgca agcatttgcc
     8281 actcttacat cgctatcttg agcttcgttc aaaaatcttg gggatttcag atctcaagat
     8341 gtacgatgtc tacacaccgc tttcatctgt tgaatacagt tttacctacc aagaagcctt
     8401 gaaaaaagca gaagatgctt tggcagtctt gggtgaggat tacttgagcc gtgttaaacg
     8461 tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa ggcaagcgtt caggtgccta
     8521 ctctggtggt tcttacgata ccaatgcctt tatgcttctc aactggcagg acaatctgga
     8581 caatctcttt actcttgttc atgaaacagg tcacagtatg cattcaagct atactcgtga
     8641 aactcagcct tatgtttacg gggattactc tatctttttg gctgagattg cctcaactac
     8701 caatgaaaat atcttgacgg agaaattatt ggaagaagtg gaagacgacg caacacgctt
     8761 tgctattctc aataacttcc tagatggttt ccgtggaaca gttttccgcc aaactcaatt
     8821 tgctgagttt gaacacgcca ttcaccaagc agatcaaaat ggggaggtct tgacaagcga
     8881 tttcctaaat aaactctacg cagacttgaa ccaagagtat tatggtttga gtaaggaaga
     8941 caatcctgaa atccaatacg agtgggctcg cattccacac ttctactata actactatgt
     9001 atatcaatat tcaactggct ttgcggccgc ctcagccttg gctgaaaaaa ttgtccatgg
     9061 tagtcaagaa gaccgtgacc gctatatcga ctacctcaag gcaggtaagt cggactatcc
     9121 acttaatgtc atgagaaaag ctggtgttga tatggagaag gaagactacc tcaacgatgc
     9181 ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc cttgttgaaa aattaggatt
     9241 ggcataaaat ggttgaatcg tatagtaaga atgctaacca taacatgcgt cgtcctgtcg
     9301 tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa gcaggtcaca ggtttcttga
     9361 aagaattgga agactttgcc cgcaaggaaa atattcctat tattccccat gaaacggttg
     9421 cttatttccg ttttcttatg gaaaccatgc agcctaaaaa tattctggaa attgggacgg
     9481 ctatcggttt ttcagctctc ttgatggctg aacatgcgcc aaatgctaag attacaacta
     9541 ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa ttttgcccag tttgacagtc
     9601 gcaagcaaat cactctccta gagggagatg cggtggatgt cttatctaca ctgacagagt
     9661 cttatgattt cgtatttatg gattctgcca agtctaaata catcgtcttt ctgccagaaa
     9721 tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga tgatattttt caaggtggtg
     9781 atgttgccaa ggatattatg gaagtccgtc gtggtcagcg aaccatttat cgtggccttc
     9841 aaaaattatt tgatgcaacc ttagacaatc cagaactcac cgcaacatta gtgcctttag
     9901 gagatggtat tctcatgctt cgtaaaaatg tagcagatgt tcaactgtct gaaagcgaat
     9961 gattttcaga aaaatttaag aaaaaatagt aaaatagata gagtaacact tatctcaaag
    10021 gagtagacat gaagaaaaaa ttattggcag gtgccatcac actattatca gtagcaactt
    10081 tagcagcttg ttcgaaaggg tcagaaggag cagaccttat cagcatgaaa ggggatgtca
    10141 tcacagaaca tcaattttat gagcaagtga aaaacaaccc ttcagcccaa caagtcttgt
    10201 taaatatgac catccaaaaa gtttttgaaa aacaatatgg ctcagagctt gatgataaag
    10261 aggttgatga tactattgcc gaagaaaaaa aacaatatgg cgaaaactac caacgtgtct
    10321 tgtcacaagc aggtatgact cttgaaacac gtaaagctca aattcgtaca agtaaattag
    10381 ttgagttggc agttaagaag gtagcagaag ctgaattgac agatgaagcc tataagaaag
    10441 cctttgatga gtacactcca gatgtaacgg ctcaaatcat ccgtcttaat aatgaagata
    10501 aggccaaaga agttctcgaa aaagccaagg cagaaggtgc tgattttgct caattagcca
    10561 aagataattc aactgatgaa aaaacaaaag aaaatggtgg agaaattacc tttgattctg
    10621 cttcaacaga agtacctgag caagtcaaaa aagccgcttt cgctttagat gtggatggtg
    10681 tttctgatgt gattacagca actggcacac aagcctacag tagccaatat tacattgtaa
    10741 aactcactaa gaaaacagaa aaatcatcta atattgatga ctacaaagaa aaattaaaaa
    10801 ctgttatctt gactcaaaaa caaaatgatt caacatttgt tcaaagcatt atcggaaaag
    10861 aattgcaagc agccaatatc aaggttaagg accaagcctt ccaaaatatc tttacccaat
    10921 atatcggtgg tggagattca agctcaagca gtagtacatc aaacgaatag tccaaatcaa
    10981 tgagtcaggg aaaaaactct acttcaggaa aaaatgaagc aaacattccc acaataaaac
    11041 gcgtagtaca aggtttgtac tgacccccaa aagttagaca aataatttat ccgaaggatt
    11101 tagttctgta ttgcacaggg ctaagtcctt ttagttttac cttaattctc ttgttgttgt
    11161 aataatcaat atagtctata atggcttgtt ccaattgatt aagtgattta aatgttttct
    11221 catagccata aaacatttcg gattttaaaa taccaaagaa agattccatc ctaccgttgt
    11281 cttggctgtt tcccttgcgt gacatagatg cttgaattcc cttactctct aggaaccgat
    11341 gataagaatc gtgttggtat tgccagtctt ggtcactatg gagaatcgta ttctcgtagt
    11401 gcttctcttt gaatgcctgt tccaacatta acgatcaatc aatttaatca tgtacctaat
    11461 attagaattg tttatctcaa atttatttga aagcttctct aagctatatc cttgttttct
    11521 aagttcatag atctgaactt tatcatcata agttaatttc ataataaaaa cactccaaaa
    11581 gttagatttt ttctgtctaa cttttggggt gcagttcatt taacacctga taatatgcgt
    11641 tttttttatt ttaaagactt tttgaccagc ctcatttttt aacttgatac tcagtgaaaa
    11701 gcaaagatta aactaggaag ctagctgtag gctgctcaaa gaacagcttt gaggttgcag
    11761 ataaaacttg tgaggtaacc aacatatata atgtgaagct gacgtggttt gaatagattt
    11821 tagaagggta tgagtctgga agttttaatg gataatgcaa gattccatag aatgggtaag
    11881 ctagagttct tatgtgaaga gtttgggcat aaacttttac cttttcctcc ctactcatct
    11941 tagtatagaa aagtgaatct aaaatagtac ataactgctt ctaaaacatt cttataaatt
    12001 gatttaaatt ctcaaatcat attattcagt tcttatttca ttttgctcta caatcctgtt
    12061 gagaagacac gtgttcatat caaaaaaggt attggcaagt tgcaatacct ttttacgagg
    12121 ctcttttatc ttatttttgt ttcaactgac tatatctcct atggttctag ttcagaaggc
    12181 taggctataa ttatgattga taagaagtat cattccaagt attgagagtg aatgtttcaa
    12241 aatcatgggt ttctataatg gtcaggctgg catttgctag accgccatct ttacgaagaa
    12301 gtggttcttt atagcctagg agagtacgaa gactggcagt aagattggcg ccgtgtccga
    12361 caattagaat acgttcagct ggactatctt ttaatgattt gataaattgg atggtccgtt
    12421 gagttgtact atagagggat tcggctccga acattcgagt gtcaaattga gcaagatttg
    12481 aacgaaaagc ctggatttgt tgcgggtaaa tagcttccaa ggttgcaatt ttcaaacctt
    12541 ctaacttccc aagttgccat tcacggagat taggaacgat ttctaaagaa cagggggtat
    12601 agagttgact ttggataatc tcagcagatt tgaccgctcg aggtaaatca cttgaataaa
    12661 tctgatcaaa aggaatttcc ttgagatact gaccaagtcg ttttagggtt tcaatggatt
    12721 caggaagaag aggagaatca ccactagcac cttgaaaacg accttcttgg ttccagaggg
    12781 tacgaccgtg gcggacaaag tagagtttca ttacttgatg tcctccaaaa tatctacaaa
    12841 gtctgccttt acaaagctag ccaagtcttg tggcgcgacg ataatgctgt gtccgacttc
    12901 gcctgcagag acaatcattt gatccaaatc tagagcaatt ttatcgataa aaatgggata
    12961 attgtgtttc tgacgaattc cgacaggatt attggctcca tgaatgtaac cagttgtttt
    13021 ttctaagtcc ttttgtggaa tcatgctcac ttttttattg ccagaaattt tagctagttt
    13081 cttttcagac aagtgctgag tgatagggac aattccgata atcggtccgg tcttgtctcc
    13141 caaaagcgcc aaggttttga aaatctgatc tcgttcataa ccttgaggaa gctctccttc
    13201 tagggcattg atttgaattc cctgatgagg gatagctgct ttagatagga tttgttccac
    13261 caatgttttt ttgattttaa ctttttttgc cattatttat atttatcctc caattgactc
    13321 atccaaatac caagccagat tcccagcgca aagaagaagg cgatgatgac ataaccgaca
    13381 agtgaaagtc ctgtgtattg gatactttca gcgtttcctg catttggaat taagatcaaa
    13441 agggtacttg ataggacgat accgatgatg aaatgataga cgcgtgagtg gtagttgttt
    13501 aaggcataat ccatcaattt tgaaaaaacg atgagagttg cacctgcacc aattccaatc
    13561 ggaaagaagg ttcccaagag atcaaaagtt ttaaaaccag tcaacatagg agcatagagt
    13621 cccaaaatca aaagtaaatt tgatgggctg aggccaggaa ccaagacgcc aagggccaat
    13681 agtgcgcctg ctaggacgaa gttaagaaag ctggcgctta aggttccaac gacaaaattt
    13741 aaggcataga gtcctaatcc agaaatgata aaggttgtcc ataaccaagt taaatcaatc
    13801 ttgtctcggt cagattctcg agttgattct ttgaggaggc taggaactgt accgataata
    13861 gctcccgcaa agctccataa tacaaagacc tgataatttt caagcaggta ttcaatcggg
    13921 taggaaaata agccgattcc tagaagcata ccgatggcaa ctggaataaa gtacaaaaca
    13981 ttttctttaa agtctttaaa gggatgggcc agaaagccaa tcattcgttc atagattcct
    14041 aagattgctg ctagagcccc tccggaaatt cccggtagga taaatccaag agcaattaca
    14101 atccctttaa taacgcgtgc taaccatgag agcatatatt tcctccaact atttctattt
    14161 caaaaaatcc ttactatatt gtatcacaat cagacacaaa aagaaaaagc aaatgataaa
    14221 caaatgcttt taaggtttta aaaaaagttt tcgaaaggtt tcttctttat tttttaaggg
    14281 agagataacg ttgatatcta aatcgtggtc aaagccggca atttttcctt tagatgtgta
    14341 ttggtgaata tcataatcta aatcagtttt aggactgctc tccaaaaatc ctgagtctga
    14401 gccgtaggac ggaatccaaa cagaggtaaa cttgcctgta tcaatactgt gttcttccat
    14461 gaagtagaca ccaacgtaga tgccgatgtt tttagcacct agtgatgcta gttttgctcg
    14521 aaagttttcg acaccttcgt tcatattaga catggttttg tcttccacgt caagccaata
    14581 gtaactaggg ctgtaaggag aggcagcatt gtagaaaact tcggcagcct tttccatttc
    14641 ttggacactt tttccagcta cataagcgta gacagcaact gggacattcc gcttttgaag
    14701 ttcagtgata tgactcttat aggccttgtc tattccattg ataaatgaag catcattttc
    14761 ttttgtcgtt tgagcaccac tgtgaacacg aacaatagca cctgaaatat tttgtgagag
    14821 ggcatcgtag ttgatttctt caggacgctg ccagccagag aggtcaataa tcggtttgtc
    14881 taagtgtttc aaagcctgtg cttcaatctg tgctatattg gattttgttt taaacgattg
    14941 gttgtcatta agtgggcgat tgatgattaa aatgaacatc ataatcccca aaaaactaaa
    15001 taaaataagt ggatgaattt gttttctcat atcttataat tctaccctaa aaatcaaaaa
    15061 aaatcaaaaa aatgggttaa ggaagagact ttagagcatt ttttcattca agagtgcgga
    15121 atgatttgaa atatggtata ataaaaggga atttctacag aaaagagaag attatgtcaa
    15181 attttgccat tattttagca gcgggtaaag ggactcgcat gaaatctgat ttgccaaaag
    15241 ttttgcacaa ggttgcgggt atttctatgt tggaacatgt tttccgtagt gtgggagcta
    15301 tccaacctga aaagacagta acagttgtag gacacaaggc agaattggtt gaggaggtct
    15361 tggctggaca gacagaattt gtgactcaat ctgaacagtt gggaactggt catgcagtta
    15421 tgatgacaga acctatctta gaaggtttgt caggacacac cttggtcatt gcaggagata
    15481 ctcctttaat cactggtgaa agcttgaaaa acttgattga tttccatatc aatcataaaa
    15541 atgtggccac tatcttgact gctgaaacgg ataatccttt tggctatgga cgaattgttc
    15601 gtaatgacaa tgctgaggtt cttcgtattg ttgagcagaa ggatgctaca gattttgaaa
    15661 agcaaatcaa ggaaatcaac actggaacat acgtctttga caacgagcgt ttgtttgagg
    15721 ctttgaaaaa tatcaatacc aataacgctc aaggcgaata ctatattaca gacgtcattg
    15781 gtattttccg tgaaactggt gaaaaagttg gcgcttatac tttgaaagat tttgatgaaa
    15841 gtcttggggt aaatgaccgt gtggcgcttg cgacagctga gtcagttatg cgtcgtcgca
    15901 tcaatcataa acatatggtc aacggtgtta gctttgtcaa tccagaagca acttatatcg
    15961 atattgatgt tgagattgct ccggaagttc aaatcgaagc caatgttacc ttgaaagggc
    16021 aaacgaaaat tggtgctgag actgttttga caaacggtac ttatgtagtg gacagcacta
    16081 tcggagcagg agcggtcatt accaattcta tgattgagga aagtagtgtt gcagacggtg
    16141 tgacagtcgg tccttatgct cacattcgtc caaattcaag tctgggtgcc caagttcata
    16201 ttggtaactt tgttgaggtg aaaggatctt caatcggtga gaataccaag gctggtcatt
    16261 tgacttatat cggaaactgt gaagtgggaa gcaacgttaa tttcggtgct ggaactatta
    16321 cagtcaatta tgacggcaaa aacaaataca agacagtcat tggagacaat gtctttgttg
    16381 gttcaaattc aaccattatt gcaccagtag aacttggtga caattccctc gttggtgctg
    16441 gttcaactat tactaaagac gtgccagcag atgctattgc tattggtcgc ggtcgtcaga
    16501 tcaataaaga cgaatatgca acacgtcttc ctcatcatcc taagaaccag taggagccta
    16561 tcatggagtt tgaagaaaaa acgcttagcc gaaaagaaat ctatcaagga ccaatattta
    16621 aactggtcca agatcaggtt gaattaccag aaggcaaggg aactgcccaa cgggatttga
    16681 ttttccacaa tggggctgtc tgtgttttag cagtaacgga tgaacaaaaa ctgatcttgg
    16741 tcaagcagta ccgcaaagct atcgaggctg tctcttacga aattccagcc ggaaaattgg
    16801 aagtaggaga aaacacagcc cctgtggcag ctgcccttcg tgaattagag gaagaaacag
    16861 cctatacagg gaaattagaa ctcttgtacg atttttattc agctattggc ttttgtaatg
    16921 agaagttaaa actatattta gcaagcgatt tgacaaaagt ggaaaatccg cgtccgcagg
    16981 atgaggatga aaccttggaa gtccttgaag tgagcttaga agaagcgaaa gaattaatcc
    17041 aatcaggtca tatttgtgat gccaagacaa ttatggctgt tcagtattgg gagttgcaga
    17101 aaaaatagag gaggtcagta tgggtaaatc tttattaacg gatgaaatga ttgaaagagc
    17161 taatagaggc gaaaaaattt caggtcctcc tttgctagat gataatgagg agactaagat
    17221 tttaccaacc tcttcttccc gttttggtta tgccaatcct aagaatcatg gttttagcca
    17281 ggaaaccttg aagattcagg tcgaaccatc tattcataaa aaccgtcgca ttgaaaatac
    17341 caagagaaat gtcttcaatt ctaagttgaa taaaatctta ttcgcggtca tctttctctt
    17401 gattttgctt gttttagcaa tgaaactttt gtaatagaaa aggaattgaa atgaaaatag
    17461 gaattattgc tgctatgcca gaagaactgg cttatctggt ccagcattta tataatgtcc
    17521 aggagcaagt tgttttgggg aatacctatc atacaggaac cattgcttct catgaagtcg
    17581 ttcttgtaga aagtggaatt ggtaaggtca tgtctgctat gagtgtggcg attttggctg
    17641 atcatttcca ggtggatgcc cttattaata cgggttcagc tggggcagta gcagaaggta
    17701 tcgctgttgg ggatgtcgtg attgctgaca aattagccta tcatgacgtg gatgtcacag
    17761 cttttggcta tgcttatgga caaatggcgc aacaaccgct ttatttcgaa tcagacaaaa
    17821 cctttgttgc tcaaatccaa gagagtttat ctcaattgga ccaaaactgg catcttggtt
    17881 tgattgctac aggagatagt tttgttgcag gaaatgacaa gatagaagcg attaagtccc
    17941 atttcccaga agttttagcc gtggagatgg agggggcagc tattgctcaa gcagcgcata
    18001 ccctcaatct cccagtctta gtcatccgag ctatgagtga caatgccaac catgaagcaa
    18061 acatcttttt tgatgagttt attatcgaag ctggacgtcg ctctgcccaa gtcttgttgg
    18121 cctttttgaa ggctttagat taagcggaaa tttgacagtt tttctagctt atgataagat
    18181 ttaagtaaag aaaagctaga aaacgtttca gaggatatta tgagtattga aatgaccgtc
    18241 agtgagattg cagaggtctt aggattatct cgccaagcaa tcaataaccg tgtcaaagaa
    18301 ttaccagaag aagacacaga taaaaatgac aaaggggtaa cagttgttac cagaagtggc
    18361 ttgattaagc tagaagaaat ctataaaaaa acgatttttg aagatgaacc tgtcagtgaa
    18421 gatgtcaaac aacgtgaact gatggagatt ctagtggatg agaagaatgc agaaattctt
    18481 cgtctttatg aacaattaaa agccaaggat cgtcagttat cagaaaaaga cgagcagatg
    18541 cgtatcaaag accgtcagat tgctgagaaa gacaaacaat tagatcagca acaacaattg
    18601 acccttcaag ccatgaagga tcaagaaaat cttaagctag agctggacca agcaaaagaa
    18661 gaagtccaat ccactaaaaa aggctttttt gctcgtttat ttggaggata atcaaggagc
    18721 tgtttaggtt aaatgctaac ctgatggctt cttttgtttg tgaatagtat agttgattag
    18781 aacagagaca gtagaggaga ggtacagaat ggaacgatta gaagattaca ttgcttttga
    18841 tttagaattt aatcagcacg aaggacaaac acacttgatt caagtatcgg cagtgcgttt
    18901 tagagatggt cgggaaatcg atgcctatga ttcctatgtt cataccagcg tccctctaaa
    18961 aagctttatc aatggtttga ccgggattac agctgagacc ttaaaagatg ctcctccagt
    19021 tgagaaagtc ttagaagaat tccaagagtt tgtgggttct ttgctgatga taggctacaa
    19081 tgctgctaaa agtgatttac ctattttagc agagcatggt ttagactata gccaacagta
    19141 caaggtggat ttgtatgatg aagcttttga acgtcggagt tcagatttac atggtattgc
    19201 caatctcaaa ttgcaaactg ttgcctcatt tcttggattt caaggtcagt ctcataatag
    19261 tttagaggat gctcggatga cggcgcgtgt ttacgaatcc ttcttagagt cggatcaagc
    19321 tagggaattg ttagagacag aaagtagctt atcaaacaat ccttttggtg gcttggactt
    19381 gtctcaatta tttgactagg agtgcctatg aaacctgaaa aacctaaaaa attgggtctt
    19441 aaacagattt acctcaatct tgataaaatc ctctttctat ttttcttgat ttttatgata
    19501 gtggagtatt tgtgggtacc attcaattca tggttggcta gcttcttatt agatcagaca
    19561 ggttatcttt ttatttccta taacaatgtc ttagccatcc ttactcattc tccattgatt
    19621 agtctagctt ttttagcctt gattgtaatt aactttctgg ttgcttattt tcaaatagga
    19681 ctcctcttta ttggtgctcg tcatctcctc tataatgaaa agcgaaggct aattgagtat
    19741 atccgcaagg ttttccatga aagttttgaa tttatgaaaa agctaactgg cgctaaagcc
    19801 ttgtttatct ttttctatat agctatgctg tttcctttta tacggaaaat gctgaagatt
    19861 tattatctta ataaaatcgt cattccagag tttatccaag cttatttaga agataaatat
    19921 tggatgtggt ggctgagcat cctacttcta tctcttatct ttctctatgt ctctgtcaga
    19981 ttgatgtttg cccttccgaa gattgtatat gatcagctga ctgttcgaga agcagtgatg
    20041 tttagcttgg agaaaaccaa gaagagagtt actttttatg cttggaattt attttacatc
    20101 ttgctcaaag cacatttatt attttatctt cctttgattc ctttattatt ggctcaaact
    20161 ttggtagatg atatcactca gagagagtct ctgattcttg gtattttgaa ttttgtcgtg
    20221 attaaaaatc tctattatat ggctctgact tattttttgg ttaaatttgt ttcatttttg
    20281 acaggaaagg agctggatat gcttcctagg agagaaaaag atcacatcgt gcgatggggt
    20341 gtcatgactt gtgccagttt tttctttgcc ttagaaggtt atatttatct ggaggctccc
    20401 atggttcatc tacctcaact tatttctcac cgaggggttt ccaatgcaaa tgggattcaa
    20461 aatacagtag agtccttgga aactacagca caactcaaac cagacttggt ggagacggac
    20521 gtgcaggaaa caaaagatgg gcagtttgtc atgatgcatg atgctaactt gaaaaatcta
    20581 gcaggtatca ataaaagtcc tcaagactta aacttggagg agcttaaagg gattgatatt
    20641 tttgaaaatg gctaccagac taaaatttca agctttgaag attatctcag tcgagccaac
    20701 gaacttggtc aaaaattact aattgaaatt aaaaccagta aaaaagatag tccagacatg
    20761 atgaaccgct ttttagcccg ttatgctgca aagctcaaga tttatggaca tcaaatccag
    20821 tctttagact accatgttgt cgaaaaagta agacagtatg atgccgaact gccagtttat
    20881 ttcatcatgc cctacaattc tgtctttcct aaaacaagag cgacaggata tactatggag
    20941 tactcaacct tggatgaata ttttgtaagc aaactatgga caacggatca gaaactttat
    21001 gtctggactg tcaatgattc agaagctatc agcaaatctc ttcacttagg agtagatgga
    21061 gtgattacag atgatttgga aaaagttcag cgggaaatag aagtagccca agaagatcca
    21121 gaatatacgg atttgctctt gaaaaaagca ttggaattct ttgaatttta gtaataaaaa
    21181 agaggaacgg ttgttcctcc catgataggt aagtattctt gtgaatgctt gcttttttgt
    21241 ggttgcaatc cgtttacgag aattgtttgt attgttcttg tttcaattta ctttatagtg
    21301 ctttgaagtt ggaatgctac actataactt ct
//
LOCUS       DAVMMR010000034        20443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMMR010000034 DAVMMR010000000
VERSION     DAVMMR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.034"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..432
                     /locus_tag="VYS74_001269"
     CDS             <1..432
                     /locus_tag="VYS74_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV3789311.1"
                     /translation="GTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTAN
                     GSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSE
                     DKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
     gene            599..1297
                     /locus_tag="VYS74_001270"
     CDS             599..1297
                     /locus_tag="VYS74_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3789312.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            1294..2346
                     /locus_tag="VYS74_001271"
     CDS             1294..2346
                     /locus_tag="VYS74_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3789313.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            2541..3152
                     /gene="rpsD"
                     /locus_tag="VYS74_001272"
     CDS             2541..3152
                     /gene="rpsD"
                     /locus_tag="VYS74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV3789314.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            3338..3493
                     /locus_tag="VYS74_001273"
                     /pseudo
     CDS             3338..3493
                     /locus_tag="VYS74_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3597..3734)
                     /locus_tag="VYS74_001274"
                     /pseudo
     CDS             complement(3597..3734)
                     /locus_tag="VYS74_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3814..4074
                     /locus_tag="VYS74_001275"
     CDS             3814..4074
                     /locus_tag="VYS74_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789315.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            4028..4249
                     /locus_tag="VYS74_001276"
     CDS             4028..4249
                     /locus_tag="VYS74_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789316.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            4568..5497
                     /locus_tag="VYS74_001277"
     CDS             4568..5497
                     /locus_tag="VYS74_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3789317.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            5511..6434
                     /locus_tag="VYS74_001278"
     CDS             5511..6434
                     /locus_tag="VYS74_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3789318.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            6692..8167
                     /locus_tag="VYS74_001279"
     CDS             6692..8167
                     /locus_tag="VYS74_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV3789319.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            8439..9425
                     /locus_tag="VYS74_001280"
     CDS             8439..9425
                     /locus_tag="VYS74_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV3789320.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            9700..10560
                     /locus_tag="VYS74_001281"
     CDS             9700..10560
                     /locus_tag="VYS74_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV3789321.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            10946..11110
                     /locus_tag="VYS74_001282"
     CDS             10946..11110
                     /locus_tag="VYS74_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789322.1"
                     /translation="MDESKYLCYLEKYDALGSGRLLAKVNNTYYMFNKNQIELLRYSN
                     KNAKKERKYM"
     gene            12546..12743
                     /locus_tag="VYS74_001283"
     CDS             12546..12743
                     /locus_tag="VYS74_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEV3789323.1"
                     /translation="MRSLSQEKDIYSFDEPTGNLDRNSTELFLNEVEKLVNEEKIVIV
                     VTHDKDVIARASKVINMDEFH"
     gene            complement(12980..14044)
                     /locus_tag="VYS74_001284"
     CDS             complement(12980..14044)
                     /locus_tag="VYS74_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789324.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(14107..15018)
                     /locus_tag="VYS74_001285"
     CDS             complement(14107..15018)
                     /locus_tag="VYS74_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV3789325.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAEDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            complement(15011..15604)
                     /locus_tag="VYS74_001286"
     CDS             complement(15011..15604)
                     /locus_tag="VYS74_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV3789326.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            complement(15591..15917)
                     /locus_tag="VYS74_001287"
     CDS             complement(15591..15917)
                     /locus_tag="VYS74_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3789327.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            complement(16056..17222)
                     /locus_tag="VYS74_001288"
     CDS             complement(16056..17222)
                     /locus_tag="VYS74_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV3789328.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            17280..18437
                     /locus_tag="VYS74_001289"
     CDS             17280..18437
                     /locus_tag="VYS74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3789329.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            18479..20329
                     /locus_tag="VYS74_001290"
     CDS             18479..20329
                     /locus_tag="VYS74_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3789330.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
BASE COUNT         6333 a         3642 c         4268 g         6200 t
ORIGIN      
        1 ggtactcaaa cttacagcgc tacagtcaat gtctatggta acaaagacgg taaaccagat
       61 ttggacaaca tcgtagcaac taaaaaagtc actattaaga taaatgttaa agaaacatca
      121 gacacagcaa atggttcatt atcaccttct aactctggtt ctggcgtgac tccgatgaat
      181 cacaatcatg ctacaggtac tacagatagc atgcctgctg acaccatgac aagttctacc
      241 aacacgatgg caggtgaaaa catggctgct tctgctaaca agatgtctga tacgatgatg
      301 tcagaggata aagctatgct accaaatact ggtgagactc aaacatcaat ggcaagtatt
      361 ggtttccttg ggcttgcgct tgcaggttta ctcggtggtc taggtttgaa aaacaaaaaa
      421 gaagaaaact aatcagctaa ggaaataaat gatggatagt gggctgacta agattagttt
      481 aacaactcaa tcagcaatca ggactttctt tcaatagcag attaaaatca tcgtaaaaca
      541 ataaaaatag tgttatactt aaagcagtat agcactgttt ttatcaaagg agagacagat
      601 gggaaagaca attttactcg ttgacgacga ggtagaaatc acagatattc atcagagata
      661 cttaattcag gcaggttatc aggtcttggt agcccatgat ggactggaag cgctagagct
      721 gttcaagaaa aaaccgattg atttgattat cacagatgtc atgatgcctc ggatggatgg
      781 ttatgattta atcagtgagg ttcaatactt atcaccagag cagcctttcc tatttattac
      841 tgctaagacc agtgaacagg acaagattta cggcctgagc ttgggagcag atgattttat
      901 tgctaagcct tttagcccac gtgagctggt tttgcgtgtc cacaatattt tgcgccgcct
      961 tcatcgtggg ggcgaaacag agctgatttc ccttggcaat ctaaaaatga atcatagtag
     1021 tcatgaagtt caaataggag aagaaatgct ggatttaact gttaaatcat ttgaattgct
     1081 gtggatttta gctagcaatc cagagcgagt tttctccaag acagacctct atgaaaagat
     1141 ctggaaagaa gactacgtgg atgacaccaa taccttgaat gtgcatatcc atgctcttcg
     1201 acaggagctg gcaaaatata gtagtgacca aacgcccact attaagacag tttgggggtt
     1261 gggatataag atagagaaac cgagaggaca aacatgaaac taaaaagtta tattttggtt
     1321 ggatatatta tttcaaccct cttaaccatt ttggttgttt tttgggctgt tcaaaaaatg
     1381 ctgattgcga aaggcgagat ttactttttg cttgggatga ccatcgttgc cagccttgtc
     1441 ggtgctggga ttagtctctt tctcctattg ccagtcttta cgtcgttggg caaactcaag
     1501 gagcatgcca agcgggtagc ggccaaggat tttccttcaa atttggaggt tcaaggtcct
     1561 gtagaatttc agcaattagg gcaaactttt aatgagatgt cccatgattt gcaggtaagc
     1621 tttgattcct tggaagaaag cgaacgagaa aagggcttga tgattgccca gttgtcgcat
     1681 gatattaaga ctcctatcac ttcgatccaa gcgacggtag aagggatttt ggatgggatt
     1741 atcaaggagt cggagcaagc tcattatcta gcaaccattg gacgccagac ggagaggctc
     1801 aataaactgg ttgaggagtt gaattttttg accctaaaca cagctaggaa tcaggtggaa
     1861 actaccagta aagacagtat ttttctggac aagctcttaa ttgagtgcat gagtgaattt
     1921 cagtttttga ttgagcagga gagaagagat gtccacttgc aggtaatccc agagtctgcc
     1981 cggattgagg gagattatgc taagctttct cgtatcttgg tgaatctggt cgataacgct
     2041 tttaaatatt ctgctccagg aaccaagctg gaagtggtgg ctaagctgga gaaggaccag
     2101 ctttcaatca gtgtgaccga tgaagggcag ggtattgccc cagaggattt ggaaaatatt
     2161 ttcaaacgtc tttatcgtgt cgaaacttcg cgtaacatga agacaggtgg tcatggatta
     2221 ggacttgcga ttgcgcgtga attggcccat caattgggtg gggaaatcac agtcagcagc
     2281 cagtacggtc taggaagtac ctttaccctc gttctcaacc tctctggtag tgaaaataaa
     2341 gcctaaaacc cctttacaaa tccagctatt catggtagaa tagattttgt gtgaaatatc
     2401 agcaggaaag catgaagctc gtcaacaggt gtcttatgac aagtaacctt ggctgtttag
     2461 gcgaagggca tctgcacgaa tcagggcttt ctaagtgact atttccaccg aaatattatt
     2521 tatatcagga ggacattcat atgtcacgtt atacaggacc atcttggaaa caagctcgtc
     2581 gccttggcct ttcacttaca ggtacaggta aagaattggc acgtcgtaac tacgtaccag
     2641 gacaacacgg accaaacaac cgttctaaat tgtcagaata cggtttgcaa ttggctgaaa
     2701 aacaaaaact tcgtttcact tacggtgtag gtgaaaaaca attccgtaac ttgttcgtac
     2761 aagctacaaa aatcaaaggc ggaatcctag gtttcaactt tatgcttctt ttggaacgtc
     2821 gtttggataa cgttgtttac cgtcttggtc tcgcgactac tcgtcgtcaa gctcgtcaat
     2881 tcgtaaacca cggtcacatc cttgttgacg ggaaacgcgt tgatatccca tcataccgtg
     2941 taactccagg tcaagtgatc tcagttcgtg aaaaatcatt gaaagttcca gcaatccttg
     3001 aagcagtaga agctactctt ggacgtccag cattcgtatc attcgacgct gaaaagttgg
     3061 aaggttcatt gactcgcttg ccagaacgcg acgaaatcaa cccagaaatc aacgaagcac
     3121 ttgtcgttga attctacaac aaaatgttgt aatattttat tgaataagat agactttgag
     3181 gccttgatat taagcacttt gggacgttct cccttagtgc ttttttgatt tctcttagta
     3241 tccagctata atcgttgaga cataactaga ccgatatagt ccaaagtgat atagtaaaat
     3301 gaaccaaaaa tagtacacaa tgtggtataa tccttttatg gcatattcaa tagattttcg
     3361 taaaaaagtt ctctcttatt gtgagcgaac aggtagtata acagaagcat cacacgtttt
     3421 ccaaatctca cgtaatacca tttatggctg gttaaagcta aaagagaaaa caggagagct
     3481 aaaccaccaa gtatagtgta ttgaatctat aacagtacac cttggctgct aaaatatttc
     3541 tataaattaa tttgactttc ctgatagaga tgttcacatc ttatttcaaa ctactataaa
     3601 agttctataa tctctttata agatttgccc atcagacaaa atagaacgat ttgaaggcgt
     3661 ttatgatatt tagctgtacg agagtctttt aaaagtgttt tgatggtttg gatttcttct
     3721 ttagttgatt tcatatcact gttatataat actttttgat tttagtctag tattagtaag
     3781 cagactcaaa ccttagaaaa tcttttgaga tcattgacag gtgtatcgaa tcctaccaca
     3841 gattcagcac gtctggtttt ggcagaggct aagaaagctt ttgcagatga tagtttgaca
     3901 gagcaaggtt tacgtgatat cttgcaaaca gtcaaagatg ccattgcttc cctagagtcc
     3961 attaaggaaa gccagtcagc aactaaggat ggaggacaaa cagcgggcaa agaaacagca
     4021 ggagaagatg agtctgcaaa tcaaactcaa gaaattagcc aaggaattaa gtaaacttct
     4081 taaagactca aaccttgaaa cagtagataa ggatgtcctt gaaaactctc aaaaagaact
     4141 tcaaaaagca gttttgttct tggcagatga aaaaggctct gagcatactg aagcagaggt
     4201 gattgataat cttaaagagg ttattgctaa gttgaaagca aacgcctaat aacatattaa
     4261 aaatctccag attaggaact atctctgagt tccctagtct ggagattttt cagtataccc
     4321 cgttgttgga tgcttataac agagtgacgc catcactggg ctaatagaga taaaataaac
     4381 aattgattta ggacatttgt ttgatagtgg tgataaagtt ttaaatctgc tacataattt
     4441 gtggagtttt ttctataatt aggagtttaa cctaacctgc aaataggagt atactaataa
     4501 tgtaatcgtt atcaaaagtc taaaaaagaa tttttagatg gatatcaaaa taaaaaggga
     4561 ggaaattatg aaaaagtttt caaaaacatt gagagacaac tggatctttc tcttgatggt
     4621 tttgccaggg gcactctggt tgattctatt cttttacatt ccagtatttg ggaacgtggt
     4681 tgccttcaaa gactaccaca tgaccagtaa tggtttcata gatagtatca taaatagtaa
     4741 atgggtcgga ctcgataatt tcagattctt atttagttca agagacgcct ttattatcac
     4801 acgaaatact gtcctctaca atcttggctt tatctttcta ggtttagttg tatctgtagg
     4861 gattgccatt atcctcagcg agctccgttc taagagaatg gtgaagattt tccaaacttc
     4921 tatgttgttc ccttacttct tgtcttgggt tatcatcagt ttctttacag atgccttcct
     4981 aaatattgat aaaggggtgt tcaatcatct attggaaagt cttggtctca aagaagtcaa
     5041 tttctacgct gacctgggca tctggcccta tctcctactt ttcctaggta tttggaaagg
     5101 ttttggatat agcagtgtca tgtactatgc gacgatcatg ggaattgatc caacctacta
     5161 cgaagcagcg acagtggacg gagctagcaa gtggcaacgt attcgcaacg taaccattcc
     5221 tcagttgact ccgcttgtaa ctgtattgac catccttgca gtcggaaata tcttccgcgc
     5281 agacttcggt ctcttctatc aaatcccaca caatgctggt cagctttaca atgtaaccaa
     5341 cgttttggac gtatatgttt ttaatggttt gactcagaca gcagatatcg gtatggctgc
     5401 agcagccggt ctttaccaat ccgttgttgg tttgattctg gttatcctat caaacttgct
     5461 tgcaagacga gtcgatccaa actcagcttt gttctagaaa ggaggagaat atggcaaaaa
     5521 agaaaattaa aaaagaaaaa attgataatg tcggcattca ctccttcagt aagaaagcag
     5581 atatcttctt cagtatcatt tcgggtttga ttgccctctc ttgtatccta ccttttgtat
     5641 tcgttatcat tatttcagtg acagacgaga aaagcctcct ccaatacggc tatagcttct
     5701 tcccatccca atttggctta gacggttttg agtttttggc acagtttaag gataaaatcc
     5761 tacaagccct cttcatctct gtctttgtaa ccgtagttgg gacattgaca aatgtcttta
     5821 tcacaacaac ctatgcctac gctatttcac ggacaacctt taagtatcgc agatttttta
     5881 ccatcttcgt ccttctcagt atgttgttca acgctggttt ggtaccaggt tatatcatgg
     5941 tgacccgtgt acttcagctt ggtgacactg tttgggcctt gattgttcca atgcttctct
     6001 caccattcaa tatcatcttg atgcgttcct tcttcaagaa gaccattcca gaagccattc
     6061 tcgaatcggc tcgtatcgat ggtgccagtg aggcccggat tttcttccaa atctgtttgc
     6121 cattgtcact accaggtatc gcaaccatca cgcttttgac agctcttggt ttctggaatg
     6181 actggttcaa cgcccttctt tacatcaaga gtgataactt gtatccattg caatatttgc
     6241 tcatgcaaat ccaacaaaat atggactaca ttgccaaagc ggtcggcttg actggtcaac
     6301 tgggagttgc tctaccgaaa gaaacaggtc gtatggccat ggttgtggtt gcaacccttc
     6361 caatcgcgat tttatatcca ttcttccaac gctactttgt aaaaggtttg actatcggtg
     6421 gtgtgaaaga atagtgctta ttgaaaaaga acatttctca gttcccaact tcccttgtgt
     6481 gaagttaata ctcttcgaaa atcaaattca aaccgcgtca acgtcgcctt gccgtagata
     6541 tatgtaactg acttcgtcag tcttatctac aacctcaaag cagtgctttg agcagcctgc
     6601 ggctagtttt ctagtttgct ctttgatttt cattgagtat aagatcatta cattgtttat
     6661 aagtttaaaa ataaaaaaag gagtttttat catgaaaaac tggaaaaaat atgcttttgc
     6721 atctgctagt gtagtcgctt tggctgcagg tcttgctgct tgtggaaatt tgacaggtaa
     6781 cagcaaaaaa gctgctgatt caggtgacaa acctgttatc aaaatgtacc aaatcggtga
     6841 caaaccagac aacttggatg aattgttagc aaatgccaac aaaatcattg aagaaaaagt
     6901 tggtgccaaa ttggatatcc aataccttgg ctggggtgac tatggtaaga aaatgtcagt
     6961 tatcacatca tctggtgaaa actatgatat tgcctttgca gataactata ttgtaaatgc
     7021 tcaaaaaggt gcttacgctg acttgacaga attgtacaaa aaagaaggta aagaccttta
     7081 caaagcactt gacccagctt acatcaaggg taatactgta aatggtaaga tttacgctgt
     7141 tccagttgca gccaacgttg catcatctca aaactttgcc ttcaacggaa ctctccttgc
     7201 taaatatggt atcgatattt caggtgttac ttcttacgaa actcttgagc cagtcttgaa
     7261 acaaattaaa gaaaaagctc cagacgtagt accatttgct attggtaaag ttttcatccc
     7321 atctgataac tttgactacc cagtagcaaa cggtcttcca ttcgttatcg accttgaagg
     7381 cgatactact aaagttgtaa accgttacga agtgcctcgt ttcaaagaac acttgaagac
     7441 tcttcacaaa ttctatgaag ctggctacat tccaaaagac gtcgcaacaa gcgatacttc
     7501 ctttgacctt caacaagata cttggttcgt tcgtgaagaa acagtaggac cagctgacta
     7561 cggtaacagc ttgctttcac gtgttgccaa caaagatatc caaatcaaac caattactaa
     7621 cttcatcaag aaaaaccaaa caacacaagt tgctaacttt gtcatctcaa acaactctaa
     7681 gaacaaagaa aaatcaatgg aaatcttgaa cctcttgaat acgaatccag aactcttgaa
     7741 cggtcttgtt tacggtccag aaggcaagaa ctgggaaaaa attgaaggta aagaaaaccg
     7801 tgttcgcgtt cttgatggct acaaaggaaa cactcacatg ggtggatgga acactggtaa
     7861 caactggatc ctttacatca acgaaaacgt tacagaccaa caaatcgaaa attctaagaa
     7921 agaattggca gaagctaaag aatctccagc gcttggattt atcttcaata ctgacaatgt
     7981 gaaatctgaa atctcagcta ttgctaacac aatgcaacaa tttgatacag ctatcaacac
     8041 tggtactgta gacccagata aagctattcc agaattgatg gaaaaattga aatctgaagg
     8101 tgcctacgaa aaagtattga acgaaatgca aaaacaatac gatgaattct tgaaaaacaa
     8161 aaaataatca gctaaattga tttcgtgtat tcattcctaa ctcctaaaat aagaatcact
     8221 gctttcctca ggttggtctg gggagggcag tggttttttt ggcacaagga tatagatatc
     8281 catgttttct cttgtcctta ttttaaccat tcaggaaatt tcgagaatcc tctacaattt
     8341 ccagtcaaat aaattgcatt cgttttctca agcaggtata ctagtatgga taaataaaaa
     8401 atttagaaaa tttaagaata gaaaagagaa caaatcttat ggcaaaagat attcgtgtct
     8461 tactttacta cctttatact ccaattgaaa atgcagagca atttgctgca gaccacttgg
     8521 ctttctgtaa atcaatcggc cttaaaggcc gtatcctagt cgctgacgag ggaattaacg
     8581 gaacagtttc aggtgactat gaaacaactc aaaaatacat ggactacgtt cacagccttc
     8641 caggaatgga agatctctgg ttcaagattg acgaagaaag tgaacaagcc ttcaagaaga
     8701 tgtttgttcg ctacaagaaa gaaattgtcc accttggttt ggaagacaac gactttgaca
     8761 atgacatcaa cccacttgaa acaacaggtg cttacttgtc tccaaaagag ttcaaagaag
     8821 cgcttcttga taaagatacc gttgtccttg acacacgtaa cgattatgag tacgacctag
     8881 gacatttccg tggggctatt cgcccagata ttcgcaactt ccgtgagtta ccacaatggg
     8941 tccgtgacaa caaagaaaaa ttcatggaca agcgtgtcgt ggtttactgt acaggtggtg
     9001 ttcgctgtga gaaattctca ggctggatgg ttcgtgaagg ttacaaagat gttggccaat
     9061 tgcacggagg aatcgcaact tacggtaaag acccagaagt tcaaggtgag ctttgggatg
     9121 ggaaaatgta cgtctttgac gagcgtattg ccgttgatgt caaccatgtc aacccaacca
     9181 tcgtagggaa agactggttt gatggaacac catgtgaacg ttatgttaac tgtggaaatc
     9241 ccttctgtaa ccgtcgtatc ttgacatcag aagaaaatga agacaagtac cttcgtggat
     9301 gctcacacga gtgccgtgtt cacccacgta accgctatgt ttcaaaaaat gaattgacac
     9361 aagctgaagt gattgagcgc ctagccgcta tcggtgaaag cttggatcag gcagctactg
     9421 tataagatca aacagtcctt aggggctgtt tttctatgct ttgtactcaa aaatctaata
     9481 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa agcactgctt
     9541 tgaggttgta gataagactg acgaagtcag ttacatatat ctacggcaag gcgaagctga
     9601 cgcggtttga agagattttc gaagaatata aagtttgttt ttgtatcttt cagaaaaata
     9661 aggtatactg tatgtaaacg atttcaaagg agtccagtta tggcgaaaac attttttatt
     9721 ccaaataaac agagcatttt aggagaacaa gagattttga atgccaagtc gatcttggcc
     9781 ttgctagacg gtttggagtc acatagctat gatgtagtct atctccgtca gcctcttaat
     9841 cgtctcgagt atattgagtg tgcgatagtg gggcaatcac aatttctctt taaggtcagt
     9901 tatgctgatg gtcaaaaggc ttaccgtgtc gatcttcctg acctactaac aaagacagac
     9961 tggcagatta tcaagtcatt tttagatgct ttgcttgctt atacagggac tgatattgaa
    10021 gggctagatg gttttgattt tgaagcttat ttccaagcaa gtattcaagc ctatctagca
    10081 gaccctgtag ctcgttttac gatttgccaa ggaattttta atcctatttt ctttagtcgt
    10141 gagaacttga aaagcttttt agaggcagat ggcttggctc agtttgaagc gcgtgtgcgt
    10201 gcggttcaag agacagatgc ctactttgcg agagtttcct tctatcagga tggagaagga
    10261 aaagtgcatg gcgtttacca tctagctcaa ggagtcaaga cagttttacc gagagaaccg
    10321 tttgttcctg cagcctatat tgagcaattg gtggataagg aagtccagtg ggagattgac
    10381 ttggttcaaa tcacaggaga cggctctaaa ccagaagact atgaatccat agctcgcttg
    10441 gactatgcaa aattcttaga ggtattaccc ccatcttttt accaccaact agacgccaat
    10501 caaatagaag tgcaacccat attagacaaa gattttaaaa cattagcaca agaaaagtaa
    10561 agcagaagca ggtcaatcga cttgcttttt tgaaatagaa aaatcctgcc aaggatgaca
    10621 ggattgctac tcaatgaaaa tcggctctat aatatttgta gtgggtaaat cccctatgga
    10681 tattatggag cctattataa cataaacgag tgacttttta agtaatgact gcgcttttca
    10741 atgatgggag aggtatggta ttggttttaa tcacaaatta tcagaaaatt ttctattttt
    10801 ttgttgacaa tattttgaat atagtataca ataatttcat taactcgaca atcactagat
    10861 atatgcaatc taatcgtaat tttataggag gagtccgctg atgtcaaacg agaatgcaca
    10921 gaatttgatg tggagataat ttaggatgga tgaatcaaaa tatttgtgct atcttgaaaa
    10981 atatgatgct ttaggctcag gacgattatt agctaaagtt aataatactt actatatgtt
    11041 taacaaaaat cagattgaac tcttacgata ctcaaataaa aatgctaaaa aagagaggaa
    11101 atatatgtaa agttttcctg ctatactagt aataccatag accaatttat tgattatttt
    11161 tacaaacaag gaatacttgt taagaaaaat tcatttgatg ataatgggtt gaagtttttg
    11221 tcaatatatc gcagattgac aagattctat attccacaaa aattaaatga ttatattgga
    11281 caaaaaaaat ctaaatttat acctttcctt ccgaatcctg tactaattac aaaagtaatg
    11341 ctatattgta gtcctatttt agctttattt ctttgttatg cttgttatga tattcaacta
    11401 aacatttcaa aagttccaag aaatattatc acatttttag taggaagcgt ggtagctatt
    11461 atacacgagc tattaataat gatatattat ttgaagtcag ggagagtacc tggacgcata
    11521 tatattagaa ttgtttatct cgtagtcata tcttttggaa cagaatgggg tgctagttat
    11581 agtgaaaaga aagtttttag aacaacgatg ttcctgtttt ctatgataag tattttttat
    11641 ttatctgcta tatttttcat gatattttac ttattacagt atttaaaact ttatacagca
    11701 gcatattata cgagcatcta tgctgttggg acattgattt ttgcatttat taatgcttat
    11761 ccattcttat taaagacaga tggttatata ctttatcaag aattttttgg aatttataaa
    11821 atacgatcat tattttttaa aacccttttt tattgtattg gaataagaaa taatctaaaa
    11881 ttgcaagaag tagtaaagag aaataaccta aaaacgtata tttgggattt attttttatt
    11941 atttctataa ttggattatt gtatatttta tatagtagta ttagatttat tatttgactt
    12001 tggaggaaaa ataaatgaat tatattattt gtgcctattc tattatttta atgttttcag
    12061 cttattttgg atataaaaaa aagcttggag tatctgttgt atcaatactt ataaacttat
    12121 gtttatgtac ttcgacattg tttaacttat tttattcaat taactatttg aaattattaa
    12181 tatcaatctt tttaatatta ctttcagtga gcttgctcta cgatagaaag attagtggaa
    12241 ataaaatcaa ttatagtcac cattgtatca gatttattat tcatgtacta ataatttgtt
    12301 atttatttct ataataacaa gagtttctat gaaagattcg acacggatag tcaactgatc
    12361 taaaatttta atactatatc tggaggtgat gaagcatgac aaaaaatatt aatttcaccc
    12421 tatttgataa ggaaaactat gaatcaaata atcttgatag tttgaatctc aatatagctc
    12481 tgagtcatca gattgatggg aaagccacca atatttcgta aggtcagaaa caaaaaattc
    12541 tattcatgag gagtttatcg caagaaaagg atatctattc attcgatgag cctacaggaa
    12601 atttggacag aaactcaact gaattatttc taaacgaggt tgagaaatta gtgaacgagg
    12661 aaaagattgt tattgtcgtg actcatgaca aggatgttat tgctagagca agtaaagtta
    12721 ttaatatgga cgaatttcat taaaagttaa taaatggaga ttggtggttt ctaatcttaa
    12781 ttttacatat aactagtttg cataatattt ggaataacga actttatagc aacgctatca
    12841 tactttgaac tctaggaata aaacccatcc taggacaaga ttttaaaaca ttagcacaag
    12901 aaaagtaaag cagaagcagg tcaatcgact tgcttttttg acatagaaaa aatcctgcca
    12961 agaatgacag gattgaattt taaagaaagg ccaagatacg aagataatct ccaatcagtg
    13021 ccacttcagc tacaaagaag aggaggataa taactccgtt cacaaggaca gacaagaata
    13081 attgatagaa ggagtcggtt tcacttgctt gacttggtct tgtaatgata tggagactgg
    13141 caagcagaat gattccaatg ctaatcacac acaagagggc cgtaaatcgt aggctatcaa
    13201 agaaagcaaa gaaactagca atagcagtga ggaagattgg aattgccaag agttgactat
    13261 attgttggag aaccttgtct agcgtccagt ccttttcctg gtggataaat cgtctcacaa
    13321 cgaaactacc caagaggaat gaaaagaaga agagtgttgt cgctactagg atagagatga
    13381 tagaaaaaag agttaaagga gctagctgct cagggaagcg actgttaatg cttgctatat
    13441 gtccatagta agcatgtttg atgtgataga tactaaagaa aaaggaagat gcagaaaaca
    13501 gaatgagcaa gagaaaggct gtgtaactgt gtgtgatact tgtttccaac ttacttgtag
    13561 gagatttgat cgcttccact agccaagacc aaaaatcaag cacttgctct ttccatttat
    13621 ccctagattt tggagcttgg tcggggatat aaggactttc taaagattta ctgataagaa
    13681 gtggctcttt cgtggttgct ttttgctgag gaagagcttc ttggctctct tcagctatag
    13741 tgactttttc tgtttcttta gaaaggtctg gctcttcttc agtagaatta gatgccttct
    13801 tttcttctat ttctgttctc gcttcaccgc cttcaggagt ttcaattttc tcttcttgct
    13861 ggctttccag ttcgacttca gcttgagaga cttcctcctc tacttgagta ttttttccaa
    13921 ttggtgtatc gagatcggct atcgtttctt cagccttgtc tgcaacctct tgagcttgct
    13981 cttcaggctt gttcttgctt gttgttttta caaaatcatt actttcaaac cattcttgtt
    14041 tcatggtaga acctcctttt tagttagata aatatgcttc catagtagca gatgtaagcg
    14101 tttttgtcaa cgtctgcttg gtgtggatat tagatcaata ttatcatcag atctcgcaat
    14161 gagttgatcc ttgacatcgg ttttttcagt tttgtaaggg ttgcttaatt ccgtacctct
    14221 tgattcaggc ttttctcttg tgaattggaa gatagaacca tagttgcttg agatgtccca
    14281 gttaattcgt tggctttctt tctggtctag gatgattctg agataatctt cggcagtcag
    14341 ttcaacctta ccatggactt ggatattttc agcgtggaag tgattctctg ttgactctag
    14401 ctgactatct gtaagaactg tatcaaagat attaacgata ttgggcgttg tgagtttact
    14461 gtttttgata cgacttcctt caattcggag gatatagctg tttgtattga gggtcgcatt
    14521 ttcaaggcta gcatttatga tggtggtttg tccgcgattg gctgagatgt tgatcccttt
    14581 tagagttctc ccttttggta gtcggagaat aacttcttca aaacgactag agtagctact
    14641 tgcgatatga agaatcccac caattccaga agagagaaac ggagtttcag acagtttctt
    14701 atcagtgaga ctcagagttc tatcgttctg attggtgata agatcatggt gagcagaaag
    14761 agatggatgg taagaaatgt ggatttgatc atcgaaagag tctgtgatgg tgagcgtgtg
    14821 ttggtggaga gtaatttcta ggttttcgac ttccttgcca aaggttagct tttccgtacg
    14881 gctatcatag acaggttctt tggacatgga aagtaggctc ttaatcccgt cagattggat
    14941 acctacaaaa agcagaataa agccgataac ggtagtcacc acaccaaaga tgagaaatcc
    15001 ttttgtccat ttacgcatgc tgattacctc tctttccttt tttaagaaca aattgtacca
    15061 gacgaacaat gagtagaccg aagaagcgag ctacatagga aatgccaagt aaaactagcg
    15121 aagaagcacc gatagccagt aaaccagaac caaaaatcaa gataaaggct gatttggctt
    15181 gggcgaggac agtgaaactt tcaactaaaa ataggaatcc gccgatgata cccagtatgg
    15241 aaactgcaaa gaaagccaga atgacagtca aagcggctac aaggattgcg aacagggtca
    15301 cgaggatggc gattcccaga ggaatgccga taggtgctgc aaggagggct aacaaggcga
    15361 tatgtaaaat ttgtcggtta tttttttgag cgggtgcttc attgattttt ttatcgagaa
    15421 gattggatag aacttcgtgg gccacttctt tgggagttcc caaactagcg atgagttctt
    15481 cttctccttc gactccagca tcgtcaaaga gctctctgaa atagtccatg gcttcgatac
    15541 ggtcagcttc aggtagtttc ttgagataga gttctagctg agtcaggtat tcagttcttg
    15601 tcatggcgga tactcccttc tatgatgcca ttgatggtgt ctgtatagag tgtccattca
    15661 tcttttaggg tcaagagctg ctctatacca ccgtttgtca aggagtagta tttgcgcatg
    15721 cgaccttgga actctctaga ataggttgtc agaaagctat tgccttccaa ttttttgaga
    15781 atgggataga gtgtggattc tttgatatta gcgatcagct taatggtttg gctaatctca
    15841 taaccataag aatcaccctg ctccagtaca gccaagatga gaaattcaat caaggcagag
    15901 gatgttggaa agtacatggg aaacctcctt ttctaatgtg taagattttt atatataatt
    15961 tttctacaca tacattgtac atctaaaaga aagccctgtc aagagaaatg tgtaaaattt
    16021 ttatatataa aaaacttcta gctaaaacta gaagtttaaa ggatcttatc cgctctgtcc
    16081 actgtaaaga gggccacagt catcaggata tcgatgagca agagggcagc tacagctggt
    16141 acccaagagt ggaataggtc aaaactgtaa ccaaagaggg ttggcccaaa ggctgctagg
    16201 atatagcctc ctgtttgaga taggccggac aattgggctg tcttttcagg ggcgcttgtc
    16261 ttgagtgaaa agttgaccat gagataaggg aagagggcgc tggttgcggt tccgatgagg
    16321 agatggatgg caagccagta aatgaaatta ttgattggga aaaagagcat ggaaatgccg
    16381 accacaccag ctagtgaaac cagagtgagc atgagctgac ggttgcgagt agataaactg
    16441 gttgtcaggc ttgggatggt cattgaaaaa ggaatgctaa tcagagataa gatagaagtc
    16501 agcaagccag cttcgtgact ggatagacct gcatggatag acatggtagg taaccaggtc
    16561 atggcggtgt aaaagatcaa ggattgaaaa cctgaaaaga taataattgc ccaaacctgt
    16621 ttattacgca tgacctttat ttgacttttt tgtttggttt gtggagctag tctatgatta
    16681 tagcggtgat ttgggagcca gaccaaaaaa gttgctagac agagtaacgt gaggaggagg
    16741 ataagtcctt tccaagaact ggcttgtgta atgggcacag ctagatagga agccagagcc
    16801 gttgcaatcc ccatagacgt tacatataag gtggtcagaa aaccaatttt ctttggttga
    16861 ttggcttgga taagactagg aagcaggaca ttgatgactg cgacacttgc cccaaccatc
    16921 aaggttccta gatagagcag gggcagattg attagtcgaa tgagtgagcc gatggtcaag
    16981 aagaagaggc tgtaggtgaa gagatgctcc aatccgattt tctgagccag ttgggtagaa
    17041 aatggtgaaa agagggtaaa cataaggaga ggcaggctgg tcaagacacc aagcgaacta
    17101 acttctacct ccagaccttg cgaaatattt cccaaaataa tgggtaaaac agtgaaagga
    17161 gttcgcaagg aaacaccaat caggataata cctggaacaa aaaagagtga ttgctttttc
    17221 atataatacc tcttctagct gattttaata acagcttatt ttaaggcttt ttcactataa
    17281 tgtcaagtaa ggtttctggc tttcaagata aaaatgggaa agtcttgaaa attatgatag
    17341 aatggtggaa ggaaaaattc aggagagtag tagtgactca aaatgttgaa agtcttctcg
    17401 tatccattgt aatcagtgca tacaatgaag aaaaatatct gcctggtcta attgaagact
    17461 taaaaaatca aacttatcct aaagagaata ttgaaattct atttataaat gctatgtcca
    17521 cagatgggac cacagctatc attcagcaat ttataaagga agatacagag tttaactcaa
    17581 ttagattgta taacaatcct aagaaaaatc aagctagtgg ttttaacctg ggagttaaac
    17641 attctgtagg ggaccttatt ttaaaaattg atgctcattc aaaagttact gagacttttg
    17701 taatgaacaa tgtggctatt attcaacaag gtgaatttgt ctgtgggggg cctagaccga
    17761 cgattgtcga aggaaaagga aaatgggcag agaccttgca tcttgttgag gaaaatatgt
    17821 ttggcagtag cattgccaat tatcgaaata gttccgagga tagatatgtt tcttctattt
    17881 ttcatgggat gtataaacga gaggttttcc agaaggttgg tttagtaaat gagcaacttg
    17941 gccgaactga agataatgat attcattata gaattcgaga acatggttat aaaatccgct
    18001 atagcccaag tattctatct tatcagtata ttcgaccaac attcaagaaa atgctgcatc
    18061 aaaagtattc aaatggtttg tggattggct tgacaagtca tgttcagttt aagtgtttat
    18121 cattatttca ctatgttcct tgtttatttg ttttgagtct tgtgtttagt ctagcattgt
    18181 taccgatcac attcgtattc ataactttac tattaggtgc ctattttcta cttttgtcat
    18241 tactcacttt gctgacttta ttaaaacata aaaatggatt tctaattgtg atgccctttc
    18301 ttttattttc cattcacttt gcttatggcc ttgggacgat tgtaggttta attagaggat
    18361 ttaaatggaa gaaggagtac aagagaacaa taatttattt ggataaaata agccaaataa
    18421 ataaaaatat gctataataa caatatagta aaactctttt aaggaggagt agatttctat
    18481 gaataaaaaa ctaacagatt atgtgattga tctggtggaa attttaaata aacaacaaaa
    18541 gcaggttttc tggggaatat ttgatatttt cagtatggtg gtttccatca ttgtatctta
    18601 tattttattt tatgggctga ttaatccagc acctgttgac tacattatct atacgagttt
    18661 ggccttcttg ttctatcaat tgatgattgg tttttggggg ttgaacgcga gcattagtcg
    18721 ttacagcaag attacggatt tcatgaaaat cttttttggt gtgactgcta gcagtgtctt
    18781 gtcatatagt atctgttatg ccttcttgcc actcttctcc atccgtttca tcattctctt
    18841 tatcttgttg agtaccttct tgattttatt gccacggatt acttggcagt taatctactc
    18901 cagacgcaaa aaaggtagtg gtgatggaga acaccgtcgg accttcttga ttggtgccgg
    18961 tgatggtggg gctctcttta tggatagtta ccaacatcca accagtgaat tagaactggt
    19021 cggtattttg gataaggatt ctaagaaaaa gggtcaaaaa cttggtggta ttcctgtttt
    19081 gggctcttat gacaatctgc ctgaattagc caaacgccat caaatcgagc gtgtcatcgt
    19141 tgcgattccg tcgctggatc cgtcagaata tgagcgtatc ttgcagatgt gtaataagct
    19201 gggtgtcaaa tgttacaaga tgcctaaggt tgaaactgtt gttcagggcc ttcaccaagc
    19261 aggtactggc ttccaaaaaa ttgatattac ggaccttttg ggtcgtcagg aaatccgtct
    19321 tgacgaatcg cgtctgggtg cagaactgac aggtaagacc atcttagtca caggagctgg
    19381 aggttcaatc ggttctgaaa tctgtcgtca agttagtcgc ttcaatcctg aacgcattgt
    19441 cttgctcggt catggggaaa actcaatcta ccttgtttat catgaattga ttcgtaagtt
    19501 ccaagggatt gattatgtac ctgtgattgc ggacattcaa gactatgatc gtttgttgca
    19561 agtctttgag cagtacaaac ctgctattgt ttatcatgcg gctgcccaca agcatgttcc
    19621 tatgatggag cgcaatccaa aagaagcctt caaaaacaat atccgtggaa cttacaatgt
    19681 tgctaaggct gttgatgaag ctaaagtgtc taagatggtt atgatttcga cagataaggc
    19741 agtcaatcca ccaaatgtta tgggagcaac caagcgcgtg gcggagttga ttgtcactgg
    19801 ttttaaccaa cgtagccaat caacctactg tgcagttcgt tttgggaatg ttcttggtag
    19861 ccgtggtagt gtcattccag tctttgaacg tcagattgct gaaggtgggc ctgtaactgt
    19921 gacagacttc cgcatgaccc gttactttat gaccattcca gaagctagcc gtctggttat
    19981 ccatgctggt gcttatgcca aagatgggga agtctttatc cttgatatgg gcaaaccagt
    20041 caagatttat gacttggcta agaagatggt gcttctaagt ggccacactg aaagtgaaat
    20101 tccaatcgtt gaagttggaa tccgaccagg tgaaaaactc tacgaagaac tcttggtatc
    20161 aaccgaactc gttgacaatc aagttatgga taagattttc gttggtaagg ttaatgtcat
    20221 gcctttagaa tccatcaatc aaaagattgg agagttccgc actctcagtg gagatgagtt
    20281 gaagcaagct attatcgcct ttgctaatca aacaacccac attgaataaa aaagaaaaac
    20341 gcatagtatc aagttacaca accttggtaa tatgcgtttt attatgtaga gacttatact
    20401 cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc gta
//
LOCUS       DAVMMR010000035        20155 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMMR010000035 DAVMMR010000000
VERSION     DAVMMR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.035"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(105..2228)
                     /locus_tag="VYS74_001291"
     CDS             complement(105..2228)
                     /locus_tag="VYS74_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEV3789331.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYTQYILAAGYLNRVN
                     DIWLENEENVENGLKKCSLEGRIDIKDLSFSYSKDSAPVIENLNLTIEPGQRIALVGQ
                     SGSGKSTLSKILSGLYKIDTGKILFDGVNINQIDKKILSQNLGVVPQDSFLLNRSILD
                     NITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIALARAL
                     INNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKDADIIFVMKGGK
                     IVESGNHKYLMDLGGEYYSLYTKRK"
     gene            complement(2221..2769)
                     /locus_tag="VYS74_001292"
     CDS             complement(2221..2769)
                     /locus_tag="VYS74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789332.1"
                     /translation="MSLSYYYEINPSTDILKCIEELLYKEDKCFNNILKNWKDIRRNH
                     NDSFPNFWCYGAPGILLARKEIFDKTNIGNNDLSIIKNVLTNVEKIRELNLCHGSVGT
                     ISCLDAILKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLGVSGVVYE
                     ISRKQDDRLLNVLLLELRGHDD"
     gene            complement(2850..2912)
                     /locus_tag="VYS74_001293"
     CDS             complement(2850..2912)
                     /locus_tag="VYS74_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEV3789333.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            2992..3846
                     /locus_tag="VYS74_001294"
     CDS             2992..3846
                     /locus_tag="VYS74_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3789334.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGEDKLKLHYTDH
                     FNKLVNKK"
     gene            complement(3891..5756)
                     /locus_tag="VYS74_001295"
     CDS             complement(3891..5756)
                     /locus_tag="VYS74_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV3789335.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKIYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVLKFHENALLE"
     gene            complement(5743..6108)
                     /locus_tag="VYS74_001296"
     CDS             complement(5743..6108)
                     /locus_tag="VYS74_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV3789336.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            complement(6118..6477)
                     /locus_tag="VYS74_001297"
     CDS             complement(6118..6477)
                     /locus_tag="VYS74_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789337.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            complement(7296..8452)
                     /locus_tag="VYS74_001298"
                     /pseudo
     CDS             complement(7296..8452)
                     /locus_tag="VYS74_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8480..9241)
                     /locus_tag="VYS74_001299"
     CDS             complement(8480..9241)
                     /locus_tag="VYS74_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV3789338.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(9241..9717)
                     /locus_tag="VYS74_001300"
     CDS             complement(9241..9717)
                     /locus_tag="VYS74_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789339.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(10162..10617)
                     /locus_tag="VYS74_001301"
     CDS             complement(10162..10617)
                     /locus_tag="VYS74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789340.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(10892..12631)
                     /locus_tag="VYS74_001302"
     CDS             complement(10892..12631)
                     /locus_tag="VYS74_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV3789341.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            complement(12794..13678)
                     /locus_tag="VYS74_001303"
     CDS             complement(12794..13678)
                     /locus_tag="VYS74_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV3789342.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(13790..14644)
                     /locus_tag="VYS74_001304"
     CDS             complement(13790..14644)
                     /locus_tag="VYS74_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV3789343.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKDYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(14702..15460)
                     /locus_tag="VYS74_001305"
     CDS             complement(14702..15460)
                     /locus_tag="VYS74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV3789344.1"
                     /translation="MVVYIRQSNLLSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIDVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(15522..15710)
                     /locus_tag="VYS74_001306"
     CDS             complement(15522..15710)
                     /locus_tag="VYS74_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV3789345.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(16032..16598)
                     /locus_tag="VYS74_001307"
     CDS             complement(16032..16598)
                     /locus_tag="VYS74_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789346.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(16866..18545)
                     /locus_tag="VYS74_001308"
     CDS             complement(16866..18545)
                     /locus_tag="VYS74_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV3789347.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEERQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(18637..18798)
                     /locus_tag="VYS74_001309"
     CDS             complement(18637..18798)
                     /locus_tag="VYS74_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789348.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(19482..>20155)
                     /locus_tag="VYS74_001310"
     CDS             complement(19482..>20155)
                     /locus_tag="VYS74_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV3789349.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
BASE COUNT         6144 a         3969 c         2739 g         7303 t
ORIGIN      
        1 actggaaaat ctaaaaaaat tgcaaatact attccaatca tcacccctag tgaggctgct
       61 gttgaaatta catattcatt tactttcttc attttcttta cacctcattt cctttttgta
      121 tataagctgt agtactctcc accaagatcc attaagtact tatgatttcc tgactcaaca
      181 atcttaccac ctttcattac aaaaataata tccgcatcct taatcgttga caatctatga
      241 gctataatta tttgagtaca accctgactt tgtatatact ttgttattct ttcctcatta
      301 atagtgtcta atgcactagt tgcttcatct aaaattacaa tactaggatt atttattaat
      361 gcacgtgcca gtgctatccg ttgcctttgc ccacctgaaa tatttgaccc catctctgag
      421 atgatagtat taaatttcat cggcatagcc atgatttcat catagatttg aactgcttta
      481 caaacttcct ctatcttttg tgaagtaact tcgttcttta aagttatatt atcaagaata
      541 cttcggttca ataaaaatga atcctgtgga actactccta aattttgact taatattttt
      601 ttatctattt gattaatatt tacaccatca aataaaattt ttcctgtatc aatcttatat
      661 aatcctgata gtatttttga taacgtactt tttcctgacc ccgattgacc gactagtgca
      721 attctctgtc ctggctcaat agtcaaattt aaattttcta ttacaggagc tgagtctttg
      781 gaataagaga aagataggtc cttaatatct attctacctt ctaaagaaca ctttttaaga
      841 ccattttcaa cattctcttc attttcaagc cagatatcat ttactctatt taagtatcct
      901 gctgccaaaa tatactgcgt ataagcatta aatattgaga tttctgaact aaataaaatt
      961 gatgccatgg tttgaaatgc gacaacctca cccaataaaa tttttccatt taaaacatta
     1021 tcaagtccaa aaatcaatat aattatagga gcgaacaatt gaaatacatt agtagctgaa
     1081 ttgtataaaa tatgcacaat acttcgtttt ttatactttg tcaatacgtt ttttaaatac
     1141 ttagaccagt tttcatatat ttcatcttct aatcccgata tttttattga agaaatagta
     1201 tttaatgctt ctgtttctac tgagtaaatc aatgattgtt cttgtaattc tctattaacc
     1261 gtatcataca ttatttttct agtactaaac agaaacaaaa aattgattaa actcaatata
     1321 agagctataa ttgataatgt ttttgaacta agaaacatat atattacaac acttagaata
     1381 gtcccgatat ctactactcc cccaacaact tggttagtaa atagctccct aaaaccacta
     1441 agactagaaa ttctgtataa aatatctcct tttgccctga gttcaaaaaa actatatggt
     1501 agtttaagca aatgcttaaa tgtaaaactt tcaatatctc tactaaaaaa tatattagaa
     1561 aatagaattc ccatactcct agccaagata gaaataagat acaaacaaag tagagaaaat
     1621 aaaattaaaa acgaggtttg aaaagaaaat gtattcccta atgcagaatt aataaacttt
     1681 gaagtcatta ctggtacgga aagtattatt aagtaactta ttattgaaaa tagtaacgtc
     1741 aaaatcaatc gaccttttac ctttgaaaaa ctagagaata ctcttatcca tggagaagga
     1801 aactgcgact tcaaagattg ataattttcg ttaggaaatg atagcagtac tatattacta
     1861 aaatgttttg aaaactcctt aaactcataa tttatatatc ctttagaagg atccattata
     1921 tagaccttat tcttttttac tttgtaaata actacataat gttgattatc ccagaatgct
     1981 attaatggta acggaagttc actaatttta tttacatctt taacttgata tgagccagta
     2041 tcaaaatttc gtttattcaa taactgtttc aagtcaccta tactataacc atctctccct
     2101 ttttctatat cacgtcttaa atctagtaat gtctctttgc ttttatagaa atgtagtatg
     2161 gacaagcagc aacataaacc acattctatt tgagacattt gctcaatatt aggtactttt
     2221 ctagtcatca tgtcctctta actccagtaa taaaacatta agtaatctat catcttgttt
     2281 tcgagatatt tcatagacaa ctccagatac tcctagcata aaagaaaatg tattcatagt
     2341 attcaaatcc gtacttaatt ctggtttgat gacctgagaa acgacattat caaaataaaa
     2401 atctatagat tcttttatta ataggttttc ttcatctttc aatattgcat caagacaact
     2461 gatagtacct acagaaccgt gacataaatt caattctctt attttttcta cattagttaa
     2521 tacattttta attattgata aatcattatt cccaatatta gttttatcga aaatttcttt
     2581 tctagctaat aaaatgcccg gagctccata acaccaaaaa ttaggaaagg aatcattatg
     2641 atttcttctt atgtctttcc aattttttaa tatattattg aaacatttat cttctttata
     2701 tagtaattcc tctatacatt ttaatatatc tgtactagga ttaatttcat aataatatga
     2761 taatgacatt gctactccag aataaccgtg tgctaaacct gttactgtta tattatctaa
     2821 ttgtgaaata caataattaa tacacttttt taaatgatga taatatccat caccaaaacc
     2881 aaaatcggta aaaattttga aattttcttc atacttggac ttcctttccg tttttattta
     2941 ttgtacaata aataaaaacg gatttaattt ttgttgggaa aatgggaaat tatgaaatca
     3001 aaacttggca tcacacttag aaaagttcgt aaaggaaaac aaattagtct atgctctgta
     3061 gcagatgaac atctttcaaa atcacaaata tctcgttttg aacgtgggga gtcagagata
     3121 tcttgtattc gactaatcaa tattttagac aaattacata ttactttgga tgagttcctt
     3181 gttttacaca atgacgacta taccagttcc gaatcatttg ctaatctagt acagtacatt
     3241 cgtaagcaat attcatcaca aagtatcaat aatatagcat gtctactttc tgatacttct
     3301 gattacactc taaattcttt tgagaaaaca atggtaaaat ctattttaca cacaatggat
     3361 tcgaacatca ttccatcaga tgaagagctt cttcatctga cagactatct tttcaaagtt
     3421 gaaaaatggg gctattatga aatcatttta ttaggtaact gtgtacgcac aattaactac
     3481 aactcctact tcttattaac taaagagatg ttaaataact atatttactc atctctgaat
     3541 aaaactaata agcagattgt ctctcagctt gctattaatt gcttcattct aagcattgat
     3601 aaggaagaat tttctaattg ctcctattta attagtaaga taaagacatt gttggacaat
     3661 gaactaaact tttatgaaca aacagttttt ctctatgcaa ctggttactt tgaatttaaa
     3721 cgttgccaat caaccagtgg aattgaaaaa atgaaacaag ccatacaagt cttcgatatt
     3781 ttaggagaag ataagttaaa attacactat actgaccatt ttaataaact agtaaataaa
     3841 aaataaatga aattattctc tatcttttaa ctaattagtc gtaaataata ttattctagt
     3901 aaggcattct cgtgaaattt tagaactgta aatttattgg tattaataat tttcacaaac
     3961 aaatccaatc ttctagctat ctcctcatat ttattaattt cattaccaag ttcctgttgt
     4021 aattttaaaa tatccgtatt gagtcgatct aacattattg attccgtcat gttcatttgg
     4081 tcaaaatcat acttagctaa ttttctagtc tcatgatctt cactcttcaa attgataagg
     4141 acttccgcca tcttgtttaa ggtggcgatt ttttcttggg tttcttctag ctgcatatca
     4201 atctctgcaa tttctaatac aagctcatct ttgatttctt ggtattgttt gttcgtgtta
     4261 gatagttcaa tcataagatt aatttcagaa atcttctctt taagactttt taaagttggt
     4321 cgtctatagg ggagtttttg gctatcgttg ctcaattgtc tgattaaggt cctacccttg
     4381 atgaaacaat tattatccat gttttcttta ttatagacaa agtaagaaga cgtttctcga
     4441 atgtagactt tatatttttt atgattgtct tcctcaaaaa tatctagctg gtagttagga
     4501 ataaagatga gtccactctg tttgatacca aacgaaacct gtatatagac accatcatca
     4561 accaacttct ctatctgatt atccgtaagt tcaatctcaa actcatggat agcatctcgt
     4621 ttttccttgt atttcttgaa ggcttcctcg atttcttcag cggagacttt ctccttatgc
     4681 tgctcctctc gaaaagtctg aaaatcttcc tgaagatttt ctaatgcttg aatatctcta
     4741 acttctctat cttcaaaata acgattaaaa aattgaacat cgtacaattt cttatcgcta
     4801 atttttttat gactcaggct tatctttttg ccatcttctt ctagggtaaa catcacattt
     4861 ttctgtttta actcgatggt tagattcaac tctttagcct ttacatgcaa ttcttctaat
     4921 gaataaacag actttagtaa aaattttaaa cgactttcaa tctcttgttt ggcaaaatat
     4981 attttaaaaa agtcttcatc atataaatct cgtttgctga gttggcgtcc tcgaattggt
     5041 tttatcatcg ttctatctgt catcaaaaaa cggctatgct tttgactaaa atcaatctga
     5101 acatgcaact gctttgcttt ctctaaaaaa tcatcaaacg atttagattg ctgtagcaaa
     5161 aaataaagac gttgtttcaa ttcaaactta tgactagatt ccctatattt tttataatct
     5221 cgataggaat aacgtttctc aataattttt gcaccagtca ctttagaaat acggtctgaa
     5281 atcatacgga ggtttcgttc caaggcatag ttccatatca actttttatc tgaattgcgg
     5341 tcaatagcgt tgattaggat gtgattatga acatgatctt tgtctgtgtg agttgctacg
     5401 ataaacttaa aacgacctcc tgtcaattcc atcatgatct cataaccaat gcggttaatt
     5461 tcttcaggtg tcagattatc ttcaggagaa aatgattgaa tgaggtaatg agcatgaata
     5521 gtttgttggt gtttttcttg tcggtcgttt ctggattcgt acaacttgtc attattgctg
     5581 aagttgacat tgtacatctc taccatttct gcatgactag ggaagtccaa gtaattgctc
     5641 atgccaaaat cagataccaa tttcaaattg tccgttttat cagggttgag aatatactta
     5701 attaaacgcc tacgatattt ttttccgtgc gtcgcaaagt gtttagtaac taccatgaaa
     5761 ctccttcagt cttttcacct ccacttgaaa ttctttgtcc acttccttaa ttaactctcc
     5821 gactccttta ctcaattctt gtaattggtt ctgagaaatc agatggcttt gattaatcgc
     5881 acgcgcaatt tgattgatgt tatttccaat cctcctcaat tcaaacacta actgttcata
     5941 agtatcagtg ttaatcgtga caaatgacat attgggatta agcaaaactt ttctggcata
     6001 gactgagaaa ttttgacaat tgctcttagc aatccgttca ttcaattggt gtaattctga
     6061 atctgttaga aatacttttt tgagattggt acgataacga taaaccatag tagcctccta
     6121 tctcatgtat ttttcacgaa acccacgact gagtgactgt acttgattga cttccgcaat
     6181 caattcttga acgcaggtca ataagattga cacgtgttct tgagtcactt ggtgattctc
     6241 tcttgcgata atcaaaacca aatgcacatc tcgactgatc tgttcaaact tttgagactg
     6301 ccaaagggaa aaccattctt tggcagctaa ctgtttgtca tcagtcatca aaattttatc
     6361 cctgacgaat ggtgaaaagt gacgataatt atcaacctcc atcatttttt caatctgttt
     6421 tagctcatct gcggttagat ttacctcttt tcgaatgtca cggtactgtt cagacatctc
     6481 ttttcctcac tttcatcctt tctcgggtaa accccgaccc cgtgtgtcat acgggaaatt
     6541 tcttaaagtc cttgttcggt ctttgcgagc tcaagcattg tggttacaat acccaaaaaa
     6601 tcatatcgcc agctaaccaa aaccttccag ttttgacagc caacgataag ataacttggt
     6661 ggcttcgccc ccaaaccccc aggtaaaatt cttagttgga tttttaaaag ttgagaaaag
     6721 tatagcagaa gaaatttggg gaagttatca caaatcaagt agggactaat ctctaacacc
     6781 actgcatgta ccgtcctata tttataatca gctcccatcg tttacatttt caggtcactg
     6841 tcttttcata ccaggaatta gaattgacaa atttaattag agaggtattc atttaattgt
     6901 tcacttcttc gctccatttc cattacagaa atttcttcaa tactatgtgt tcagctgact
     6961 cccatgattc gttgtactac gtcctaaacg ctcacggaat aagccgtaca tctttcctag
     7021 tttactctcg taatttacgt atataggtta cgacacctat ttggatttta gagttcctca
     7081 tccgctatat atggcttcaa gtcacgattc tgtttgtaga accttgattt cgctaattcc
     7141 ttcctatccc ccctacctca cgatattcag gcttaggagt cgctattggt ttcatctgca
     7201 gcaaatatcc acagaggact tggacctcag atgcacacta tgcctgtcgg acaaagaaaa
     7261 aagtaggcta aatttctaac ctactttttc tgactctact ctgataaagc cgtctcatat
     7321 atagacattt tatgcttgaa ggaatctttt cctccctcta attgattgat gatatgattt
     7381 tttatatatt tttcactaac atatccctgt gattcaatcc atccgttaac tccatctgac
     7441 tccataataa caacttgatc tgtcatagaa tttgttacaa ttgtagcatt atgtgttgct
     7501 aaaataattt gacgttgagc tttcacatct ctaaactgct gaactaaatg cctgcaaata
     7561 taacgattgt ctagattgtc ttcaggctga tcaataatca atggtctgaa gtctttagaa
     7621 taatcactat atgctaataa aaaatcaagc atggctacaa ctttttgtcc caaacttaat
     7681 acaccaactt ctttatagag aatatcaagt tgttgcgaag attctttcga gttaatatta
     7741 aactccaaaa tcgtgtattt tctttagaaa aaatttttcg tttatatgca cctgataagt
     7801 gagaaccttt tttcaaaatg tcataactat tgaaatgagc aatatagtta actatttttt
     7861 gattattgaa atccttcatt atagtcagtg aatgttgata acttccatct ttctcactta
     7921 taatattttc acttaaccat tgattaaccc atgattcttg ttcataatca tatatacata
     7981 caatatgcaa tttatctgct gcactaattt ctactccatg taaaatatga ggatgtatat
     8041 cataaatcgg ataatttttc attatgattg agactgccat ttgtaacttt ttaatacctt
     8101 tggtagtatt atgatcagtt actacaacta tttctattcc attttttatg attgcctctg
     8161 ctaacataag aaaactaata tattcttttg aactagaaaa aacaacctta tcaaaattac
     8221 taaaaaaaat tgtcttatca acttttattt tcttattttc aaaaaatata tcatatagtt
     8281 catcttcagt aatttttcta tatttcgttg cagtccaatt agaaaatagc ttgtaatcat
     8341 aagaaacagg tgtatgcaaa tgaattaaag tcttgtgaaa gctaccgaat ttctctttgg
     8401 aatttgttag ttcttgataa acagacttta tcttgttatt aaaccctctc atttctattc
     8461 tcctaaaaat atcaattgtt tatttttcaa gtaattcatt aagtctcttt tcccccacct
     8521 gcaacatctc cttctctacc tgactccact ccccaaagag taactcttga agaacatctg
     8581 ctgctgaagt tgtattttct tttgaatcat atacacaact tctatctcgt tggtaaattt
     8641 gaatgctttc aaagatagcc agttcttcca attgtcgtgt attatcaact agatgattta
     8701 caatgaaatc atgatgttct tttggagttg cgcgtgcttg atttggattg ataatgtaca
     8761 gttcttcata acggataaga gtacttagat acgacaattc aggctttgtc gcaattaagg
     8821 ccaattgtac ttcatatccc ttatttttca agagttgtgc tgttttcttt ggaacatcaa
     8881 ctgttcgtaa agttccctcg atcaaaagat tgtatctcaa actactcaat tttgttacta
     8941 aagactctac catttttcct gcaaaatctt tggtatattc tacactgtct ttgccatatt
     9001 cttgctgcag ttctaaatag tgtggatgct gagaacgaaa actatcacca tctatgataa
     9061 caatatttcc ttgaaattct ttctgtttaa tacgatgaat tgtagtctta ccggcaccac
     9121 tttgccctcc aagcaaaatc gctataggtt gcttactgga cttttttcct cttgtcagtg
     9181 aacgaagatt ccttgctaaa gcatgtttga attcactatc agtataatct tggatttcca
     9241 ttaagctacc atccgctttt cagatatctc taacatacgt tcaattccat ccaaatagcc
     9301 actatatctc tctatttcat caaaagtttc tactaaatag atattcgtat gaatcaagtc
     9361 agatagatca tcactcatta aaatccaagg attagattca tcatcaatgc taattccctg
     9421 actatcttga taacgataga gtcgagataa tagattagca cctctttctt tcacaatttc
     9481 aattttcaaa gtcaattcat aatcttcaac aggattgagc attttatctt ctcctacaat
     9541 atcgacataa gatacattaa acttctgaca aatgatgtct attagttccg tagagactga
     9601 actagttcca ttttcatacc ggctcaagct atttcgagaa attcctacaa ttcgggaaaa
     9661 gtcgtcttgt gttaagtcat gtgttttacg taaggatttt atgttctttc caatcatagc
     9721 aaactccttt atttttataa atccattata gcataaaaaa gcataacgca ccaaaaatgg
     9781 tgcgttattt cttgtttttc tatcctaaac tttacttcta taaaagtcac ctaaagagct
     9841 ataaatattt attcttgtat taattttttc gatgcgcaaa caatatctac aaatacatct
     9901 ataacgattc accttttagg atttttattg tcaaaattgc ctttatctac aaagacaaaa
     9961 tgcttataaa atagcttgaa attaaggatt atcacctttt tcgttgtagc aacactccac
    10021 atgtgattat taggctatta gcaaagcgta tccaatacct caataaagtg ttgtttttac
    10081 tgatatttta actatattgt atagtctact atttaaatct caattaggat attttataga
    10141 atacccaaaa ttcaacaata actaaaagta agattccatt gtatttcctt gttggtcttt
    10201 catatcacga attttatttt tcagaatatt aaagttacaa atttcatctc taatatcgtt
    10261 atccgctctg tagccattaa tcatctcagt cagagttttc cctttactac cattctttat
    10321 agtagtattt actcttttta tactgctttc aaaaggctca tatgttttat acttaattac
    10381 ttcgtcttta aaattaaata aaggtgtatc cggcttagca ttccataaaa taatcaacca
    10441 cattatagca ttataaagct gctttgcagt ttcattccac ttttcaggtt gtaaattttc
    10501 actccctgta ttgtcatatt cttgtctatt atttacatga gaatcaccta caagcggatt
    10561 aaaaagaggt agccctttgt gattaattag ttctaaaaaa tcccttgccg cacccatgca
    10621 acactgaata acttccttct tgccatcaat tttactctca gcgataaata cataatattc
    10681 atcttcaata gttgctcctg catcactatg tttttgttga tttgctaaca gcttaatatg
    10741 tgctttaggc taaacttcaa aatctctaac aattctttgt cttgtttcat gtcctctaca
    10801 attcatttaa gttctccttt acaagtttag taactagcct tattatacta tataagatat
    10861 gaaatgtacc ataatcttca tcaactagtt tttagaaaat cacaaatcca tctggagaga
    10921 tgacaagctg attctccaat tcttggcaat tgcttgctag tgctactgct tctttctcta
    10981 aaagataatc ttctgttgat tggttgaata ttgctttgag gatccgtcct tcgtatttcc
    11041 attccagagc tactagatca gatttgattt cttgaaggct atacttgcca tgcgaaatga
    11101 ttactgacgc gtatttccaa atcttaatca gcctcttcat aaagttcagc atatcattgt
    11161 cacttgatac acgttcccaa ggcatacaac gacgacaatc tggatctggt cctccagtca
    11221 aggctagctc ggttccgtaa taaatgcacg gtgttccttt ttgtaaaaag agaaaggcta
    11281 aggctgattt aaccagttga acatcttcat tggccgtcca caggattcgc tctgtatcat
    11341 gtgaatccaa gagattaaac atgacctctg aaatctgctg cttgtaatac atagactctc
    11401 cattgatttc atcgatgaac tggtctgtct tcttaattcc tcgtaagaaa tagtccttga
    11461 tactatcaga taaaggataa ttcatgacgg catggaactc atctccattt agccaaggct
    11521 gagatgtatg ccagacttct cctaggatat aaagatcagg atttttagct aaaactgcct
    11581 tacgaaaatc cttccagaac tgatggtcaa tctcattagc cacatccaaa cgccaagcat
    11641 cgatattaaa ctcttcaatc caataagtcg caacctttaa aagataattc ttgacctctg
    11701 gattggctgt atttagctta ggcatatagt cctcgaaacc aaaaacatga tagggtaagt
    11761 ctctcttatt aactagcttt tcagttgtca ctgggaattg ttgaatatgg aaccaatcct
    11821 tataagcaga ctgttcacca tttttgacga catttttcca ttgaagagat tgcgaaccaa
    11881 tatgattaaa taccgcatcc agcatgactt tcatgccacg atgatgcgct tgatccacca
    11941 gttcccgaaa ggtctccttg tctccaaaat gacggtcaat ttcaaagtaa tctgtcgtat
    12001 tgtacttgtg attgcttgta gattcaaaga tgggacaaag atatagtcca gtaataccca
    12061 agtcttgcaa gtaatccata tgatcaataa tcccctgtaa atcaccacca aagaaatcat
    12121 cgctcttagg tgtgacagat gaatcccagt ctaaagtccc ttctgggttt aatagagcat
    12181 tgccattggc aaatctttca ggaaatatct gataccatac cgtatttgaa acccagtcag
    12241 gaaccttgca ggcatcaatc tcatgaagat aaggcaactt aaatccattc ccaattgcat
    12301 gaagattttc tagagaattt tccacacacc ctttatcgcc atacaaaata ttttgacctt
    12361 ctgtatctct gagctcaaag agatactgga tacgtgcaaa gtcaactgac acttcaacct
    12421 gccaatggtc aaataaggta ccagaagtta tcttgaccat ttcttttgta tcctgataaa
    12481 actcctccat aaagataaaa gggtccccat agtgcaagtt gatgctttca atgtcccctt
    12541 tcttagttcg aattcgaata tggagtttct tatccttata aagataggca tactccgact
    12601 caggcctatg gtaaatagca ctcaattcca tcaatttgtc tcctaaagta ttcattcaaa
    12661 ttttacgcaa acgttttcaa gctcaatgaa tccagtatac tatttttgta aactatttgc
    12721 aaggaatgtc cgttgttaaa ggaactatct tttaaagtat acaaaaagcc ttaaaaaagg
    12781 ctttctctat atactaatcc tctttcggtt ttgaaaatac ttctactcgc tgagctagga
    12841 ctttgatttc tacaggtagg ttatctgatt tatctccgtc aacatctgac tccaattcac
    12901 tatctgaaga gattttaata ttacgcgctt tgatatactc gatattatca tttgcgacaa
    12961 catccccttt taataaatca ggaatgacgg ataatttgga gaatatagag gcatctttca
    13021 gaatcaaaat gttggcatag ccgtccttgt tttcttcaaa gattttctta tcagcgaagt
    13081 aatttgtcaa aaggaccaaa acatggctag cttcaccaac ataatttcca ttttctgtct
    13141 caaccttaat gttaaagacc tgatctgtca tgacagactt catggtattt acagcatagg
    13201 ttaaaatacc gaattttgtt ttgtcctcta tttcaacatt gtgaatcgcc tcaggcagag
    13261 aaccgatact aaagatataa ccaaaatagt tgtcatttgc tttaccgata tcaatcttat
    13321 tggttaaatc aaaatccagt tcgtcaattg cgccatcgat gtcttgattg atttccaaaa
    13381 gttttgtaat gaggttaccc gtaccgcctg ggataatccc taacttagga atgtagtctc
    13441 tctcatcaat acctgaaatg acttcattga cagttccatc tccaccaaac acaaccactg
    13501 catcatactg ctcacgagaa gcttcttcag caaaatgtgt tgcatccagc gctttttcgg
    13561 taattttggt ttcaacatat tcaaagtatt cttttgcttt attctccagc ttttctttgt
    13621 aatccaaagc cttctcgcca ccagaagtag ggttgataat taccattgct tttttcattg
    13681 attttatcct taattttaaa cagaaatgtt tacatttcgt cgtatgcaag taaatgtaat
    13741 cctattatac aatgaaaata cagaaaagag aaatctgacg tactggagat taatacgctt
    13801 ttattctatt ttcccatcgc ctaactacat cctttaaggg ttcatccaag taagaatagt
    13861 ccttatcctt gatccaatca ggaataccgt aagctgcctc tgctatgcta caagtgattg
    13921 ctgcgagagt atcactgtcg ccaccaagtg agatggcatt tcttatcgca tcttcgaagt
    13981 ctctactttc aagaaaggcg ataatggctt gagggacagt ttcctgacat gtttcgttaa
    14041 aacgatagtt aggacggatt tcatctaaag tttgagatag attgtaatcg tattcttttt
    14101 ctatataatc cttaattcgt cttttacact ctgtaggatt gtcgttaatt gattgctcgt
    14161 actctctaca ataaccacca aagtaaaaac gacatagaaa gatagcatca gctgtagcca
    14221 tagcgccttt gacaccttct ggatgattat gagttacctc tgcagaaaga ctcgtaagtc
    14281 ctctagatga tggccatata ccagttttcg cataaaaacc acagtccatg atccaagcac
    14341 atggagaaat acgcatagct gatccattcc caaagctatt ataaggctca cggttatcgc
    14401 tgtttagcca cgcattaaac cgagcaccgt aatcagcatt cggatacatt ctgccatatt
    14461 tcttcatcgc gtcaatgaag tcgtcttttt gtccaccatt cataattgct tctgcaacag
    14521 cacaggtcat aaccgtgtca tctgtaaaaa agcagtcctt ccgaaataaa ggaaagtcct
    14581 ttgttttgat attgttccat tcgtaaacag aaccgacaat atctccaata attgctccaa
    14641 gcatcagatt cctccttgtt cattttgatg ctttttatat tggttatcta ccatatttat
    14701 tttagaaaat aacatcctgt tggattttaa aaatttcatt tttttcaaaa tagggtttta
    14761 ccatttcttt ccacctagct ctatgaaaat tgattgattt taaaggagat aggccataat
    14821 ttcccaatgc ataaccatca tttacttcaa caacaagtgt tctgccatcg cgagtaacac
    14881 cgatatctag tccataagct attggcgcat ctttccaaca tgatatcgct tcatcaatta
    14941 cacttgcatc aaattgtgca tgataatcac ctgtataggg tcgaacatct aatacgcgac
    15001 catctaacac aaaacaacgc cattcagcta tgaattctac aacctcacta atccatatag
    15061 gatagtcgaa aggtagacca atacctatta aatcatgggt tccattaaca actcttccag
    15121 taaagacttt tgaaccagct ttaggcttaa taaattttcc ccaattatca ggtatattca
    15181 caatctctcc taaaatacca gcataaatct ttcgaccata aaactcttta agctcaatag
    15241 gatagtcata aaccggaacg tttaagccca tcatttttag taatgctcta gtctccatta
    15301 tataatctac atctatatct tcacttgtta actcttttat ttcagaaaaa gattgatata
    15361 aaataacttc ttctccttgt aagtaggcac ctacttgagc attgtattta ttaattgaaa
    15421 cctcacttag taagttactt tgtctaatat aaacaaccat ttcatcactc ctatatcact
    15481 agtgttacac caatttgtaa aaaataatag caattttgct cttatttttt tgagtaaata
    15541 gcccccataa tatcatcgaa ataatcaacg gtatttagga gtaattcaat aacctgggac
    15601 tttgttagtc gcattcccct tctatctcta gcatcttcta ctaaattttc aagtttctct
    15661 agatttttat catccaagct aatcattatt ctatttttat cggttgccat tttcatcacc
    15721 tcaagttaat tctatcacag gtgtaacact tgtgtcaact ggcttttata atacattagt
    15781 ttaaaagtgg agaggatttt taacacagta actttaaatc tttggtatta aaaaattttc
    15841 acaatattta tagaaataaa atctgtctca aatcagttat caaatctagt ataaattatg
    15901 agcggctact ctaatacttt ccctctaaac aagaaaaaga cttacactca agggttttct
    15961 tccccccctt cgttataacg ttttgactct tttactagca aaggtatata ctcacaagga
    16021 actttggttg actattgaat ctctccaact tcttctttaa catatccttc tacatcttca
    16081 atctctacaa acattgggtc taagtgacac aagaaatgcc aaacttcgat cccttttttt
    16141 ctgtaaagaa tcgcttcacc gtcttcactt ccgaaaaagc ttctgtcgat ttcatatccg
    16201 cggctttcta agaagtcttt tgctttacga tagttcgttt ctcttgtttc gacataggct
    16261 ttaacttcat ggttgttaac gacatatgca tcaatttttg aatatccttc gatcactcta
    16321 tcatttttga gggataaatt tgaaatctct ttccaaataa tgtttacatt ttcctcagga
    16381 tcgaacataa atttagataa aggaacaata tttccgttaa aaataatttc catataatcc
    16441 ggtatgtttt taggattaaa atactccact tcaaaaccat cttctgtttc cagagtgtat
    16501 cccgggattt gagctacaaa ggctttccca tcttctatgg aatcaaatgc tactaaatct
    16561 ttagaataat cattttggta caattccaat ataaccatcg ataatctctc cattttcatt
    16621 atcaggctaa tgtaaataag cacgtcacct gaccaattca ggctctctgt atcatctcat
    16681 catatttcct acttacttta cgagtcttat acccagaaca caccttatcg accttcggtc
    16741 tcacctcgtc gcattggctg aacatctact tttactttgc tgatgcttca actcgtacaa
    16801 gcagtgatac cgcctcagtg tgatgcgtca gtgggactca aaaggttcgg ggaacctttt
    16861 gaggattaac tacgtttctc taataaactt acacattcaa cttgttcatc attgtccaaa
    16921 cctatgttga gattttcttc tataattggt agcttaaaag taatggattt tagccattgt
    16981 ccgttagatt gtctttcttc ataaacttga atttcagaaa tcaaagctga aattaactgc
    17041 ctacgctcta catcattcat gactttatag agcttatcaa aatagattag aaccttatat
    17101 atgttatctc ctgtaagctt ttcagcttca atagtctgtt tctttgcttt cgcatcaatt
    17161 agtgatgatt ctaattcatc tattttgtca tacatacgat atagtctatc atctaaatcc
    17221 tgtttccttc tcttataatg cttatcttca acatctaaat tatctatttc ctcaattagc
    17281 ttaaactttg tagaatgact ctttctcaat tccttttggt aattatctat ttctttttct
    17341 atttcagagg tatccacctt catgttgatt ttttcttgca tcatagaagc aaatttcgga
    17401 ttacttacta tcttgacaat cacctctgca acagcatcat ctaacaattc ttctctaatt
    17461 tgcttactga atgtacactt atgacctctt atcatctgcc tatgtttaca accatagtaa
    17521 taaaaatctt tatactttgt gccatctttc tttttcttga tacacttgtt cccaaacatt
    17581 cccactccac atatcgggca ttttacaatt ccagaaagca agtgtgtgcg tgtatctttt
    17641 cctttattca catgctcata tttctttgct tgagatttta gcttaacctg agcagcttgc
    17701 caaacttcat cggaaactat agcttcatgt atcccttcag atattagata ttcatcttgt
    17761 tcaacctgct tatattcatt tcttgtacca tgaacttttt ctaaagttct tcttccaaat
    17821 gctattttcc cattatatac aggattcttt aatatctttc ttataagacc tgcatcaaac
    17881 aaaggattct taccattctg tcttgggatt tttctaattc catgattctc taagtattta
    17941 gatatcccat tggctcctat cgtagtattt acatactggt cgaaaatcgt tcttattgca
    18001 actgcctctt cctcatttat aaacagcttg ccgtcttcaa gtttatatcc atacggagca
    18061 aagccaccat tccattttcc ttcccttgct ttttgaatgc aaccttccat tgtttgaata
    18121 cggatgtttt ctctttctat ttcagccaca gctgataaaa cagaaatcat tagtttccca
    18181 gcatctttag atgaatcaat gccatcttca acgcagataa gattaactcc ataatcctgc
    18241 attatctgaa gtgtagaaag aacatcagcg gcatttcttg caaatcttga taacttaaac
    18301 acaagaacaa aagatactcc atcttttcca gattttatat cttccatcat tcgattgaac
    18361 tgtattctac cttcaataga cttgccagac ttcccggcat cttcatactc tccaacaatt
    18421 tcataatcgt tgtaaatagc aaaagctttc attcgtgatt tttgtgcctc taacgaatac
    18481 ccctctatct gtattgacgt agatactcgt gtatagaggt atacttttat tttttctttt
    18541 gacatagtat taacctcaat ataatttttc tatatcatat ataatttttt taatttaagt
    18601 ttggactatc atttcaagta tattataaca cttttattag tccgtctcaa tttgtgtttt
    18661 tgccatgtca aaactatttt tcatctcttg attttttgct ggcgttggat cgggtagatt
    18721 atctaaatct aaagcaccag catattttgc aatcagattt gctattaaat cagccaatcc
    18781 attccagtca ttgtccaata tatacctcct ctaaagtttt atatctaata attatttgtt
    18841 taattaagtt ttttgacatt gacaagtgct ttggattagc aacataggaa tctcacctcc
    18901 gcctctattc cggatgagcc ggcttcaacc ttagaagtat cattatcctc attttcttca
    18961 tagcggatag ggtatccctc cctatattca aactcttact tatcgctcac tttctttttg
    19021 cttagcagaa ctttttttgc cgaattattc agccgaaaga tcttgacgga taggttatta
    19081 cgctccaaaa ataattaacg tcttgtcttg gtctattcaa ttgttaaggt tcaaaattta
    19141 tcgaaagtta ttaatctttt taaaatttga ccatcagaaa atatttatct tgatgtaaca
    19201 aaattctata aattaccctc ttatacttaa cagtgaaaag aagtctttct tggtaaccaa
    19261 ttttgaaata gaatttgctt atataaaaag gtccaattcc tactgcataa gtagcagtga
    19321 aaattagacc ctcttggtaa ctgtcatcta aaagtcttct agcatttctt taattttcta
    19381 atcgccttta aagtgtcata tttatatttt tttatctctg gcatactttg ataccttttt
    19441 aaacttaaag tctttaatag tgcctttcca cctcttttta tctataaaga ttctcctaca
    19501 tcataattca tttttttatt taaacctttc tgtcttagtt tgtctttatc ttcttcatac
    19561 cattttaaga ttgtcacata gtggttttga taggtcttac cactgctttc catgtatctg
    19621 gatagtttat ttatcattat atctgtgtgt gagtttaatt tttcttttag atttttatat
    19681 tcttctttgc ttaaccttac atttttgaat tctccataaa aaatgggggt ggacttttta
    19741 tctatctctc cctctctctc tttatctatc tctatatctt tctctatctc tatctcttgt
    19801 cggacatggg ttggactcat taaggacaat gtcctctgtt tattttgtct gtatcttctt
    19861 tttttctctg cccaggctgt ttctgatccg attaagtttg gtatttcagt taggtagtat
    19921 tcatcatttt ctgttacaac ttctaataag ccttgattga ttaaataatt tactgttacc
    19981 attacatcat cgtctgtttc atctatggtt agtgctaact cttttataaa atcactttct
    20041 actccgtcat aatagagttt gccctcatct tttaggctta gtagtagaag ttttaggtag
    20101 attatcgtat aggtatctcc tccagatatt ctccttaacc tttttatcct tttgt
//
LOCUS       DAVMMR010000036        19261 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMMR010000036 DAVMMR010000000
VERSION     DAVMMR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.036"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            85..3435
                     /locus_tag="VYS74_001311"
     CDS             85..3435
                     /locus_tag="VYS74_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3789350.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQVLLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3941..4411)
                     /locus_tag="VYS74_001312"
     CDS             complement(3941..4411)
                     /locus_tag="VYS74_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3789351.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4428..6701)
                     /locus_tag="VYS74_001313"
     CDS             complement(4428..6701)
                     /locus_tag="VYS74_001313"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV3789352.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7048..10149
                     /locus_tag="VYS74_001314"
     CDS             7048..10149
                     /locus_tag="VYS74_001314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV3789353.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10232..11239
                     /gene="pfkA"
                     /locus_tag="VYS74_001315"
     CDS             10232..11239
                     /gene="pfkA"
                     /locus_tag="VYS74_001315"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV3789354.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11298..12803
                     /gene="pyk"
                     /locus_tag="VYS74_001316"
     CDS             11298..12803
                     /gene="pyk"
                     /locus_tag="VYS74_001316"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV3789355.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13538..13763)
                     /locus_tag="VYS74_001317"
                     /pseudo
     CDS             complement(13538..13763)
                     /locus_tag="VYS74_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14611..15483
                     /locus_tag="VYS74_001318"
     CDS             14611..15483
                     /locus_tag="VYS74_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV3789356.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15629..15973)
                     /locus_tag="VYS74_001319"
                     /pseudo
     CDS             complement(15629..15973)
                     /locus_tag="VYS74_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16116..16427
                     /locus_tag="VYS74_001320"
     CDS             16116..16427
                     /locus_tag="VYS74_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789357.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            16431..17147
                     /locus_tag="VYS74_001321"
                     /pseudo
     CDS             16431..17147
                     /locus_tag="VYS74_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(17277..18092)
                     /locus_tag="VYS74_001322"
     CDS             complement(17277..18092)
                     /locus_tag="VYS74_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV3789358.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18089..18994)
                     /locus_tag="VYS74_001323"
     CDS             complement(18089..18994)
                     /locus_tag="VYS74_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV3789359.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
BASE COUNT         6001 a         3303 c         4106 g         5851 t
ORIGIN      
        1 ttgtgagata gctataaaag tgtggagaaa tagcttgaaa tttagtataa tatagctaaa
       61 ctatttgttt aaagtgagaa aaaaatggga aattttagct ttcttttaaa aaatgacgaa
      121 tatgaatctt tttcaaaacc ttgcattgaa gctgagaata tgattgctac atcaactgtg
      181 gctactgcct ttatggcgcg tcgtgcttta gagcaggctg tccattggat atatagtcac
      241 gattcatatt tagaagctcc ctatcgtgct actctatctt ctttagtatg ggatgatgat
      301 tttagggata tcgtagattc tgaactccac aagcagatag ttctgttgat tcggtgggga
      361 aaccatgctg ctcatggtgg tgaaattaag gaacgagaag cgattttagc tttgcatcat
      421 ttgtatcagt ttgttaattt tatcgattat tgttacagca atgagtttgt ggagcgttat
      481 tttgatgaga agtgcttacc actttcagca aacatcaaat accgagaaac tccacaatct
      541 atgataaagt tacaagacag tttaccagaa ctgcctgatt ttcatgaaca gatggctgct
      601 cagtccgtag aagttcaaga gacttatact gaaaaacgtg agactgcagc gcaacggcaa
      661 gatgtgcctt tccatattga tcaattatct gaggcagaga caagaaagct ctttattgat
      721 atcgatctcc gtttagcagg atggatattt gaagaaaact gtcgtgttga gatagccgtt
      781 gatggtctca agcacggttc aggaattggt tactgtgact atgtacttta tggtaaaaat
      841 gggaaaattt tagcgattgt agaggctaaa aaagcctctg tcaatccaga agtaggggaa
      901 gtacaggtca aagaatatgc tgaagctctt gagaaacata tcggctatca gccaatttgc
      961 tttattacaa atggcttgaa gcactatata cttgatggtc cgaaccgccg ccagattgca
     1021 ggcttttact ctcaagaaga attgcaatta gtgatggata gacgtcatct tcaaaaacca
     1081 cttgaggata tttctagtaa aattagggac gatatttccg ggcgtcacta ccaaaaacat
     1141 gccattgcaa gcgtttgtga agctttctct aatcatcgta gacaggtact tttggttatg
     1201 gcaactgggg ctgggaaaac tcgtacagca gtttctctag ttgatatctt atcacgtcat
     1261 aactgggtaa aaaacgttct cttcttagcc gatagaactt ccttggttaa gcaagcctat
     1321 gattcgttta gaaaattact cccagatctt tccgtttgta acttcttaga agataaagaa
     1381 ggagctcaat caagtcgcat ggtcttttca acttatccga ccatgattgg agcgattagt
     1441 ggtcaagaag aagtaaatca acgccctttc actgttgggc attttgacct tatcataatt
     1501 gacgaatctc accgttctat ttatcagaaa tacaagtcca tttttgatta ttttgatgca
     1561 agaattgtag gcttaacagc tactccgcgt caagatttag ataaaaacac ctatggattc
     1621 tttaatttgg agaatggggt tccaacatat gcatatgatt tggaagaggc tgttaaagac
     1681 ggatatttag tagcctatca ttctatcgaa accaaactga aactacctac ggatggtcta
     1741 cattatgatg atttgtccga agaagaaaag gaacattttg atagcaaatt tgaagacgat
     1801 agctgtgaaa aagatattga tgggagtgta tttaattcct ttgttttcaa taaaagtaca
     1861 gtagaaattg ttttaaatga actcatgaca agaggaattc agacagcctc gggtgatgaa
     1921 attggtaaaa ctattatttt tgctaaaaat catgatcatg cggaatatat cagaggtatt
     1981 tttaacaacc gctatcctga aaaagggagc gactatgctc aggtgattga ttatagtatt
     2041 aagcattatc agaccttgat tgatgatttt aaaattaagg agaagtatcc tcaaattgcg
     2101 atttctgtcg atatgttaga tacaggtatt gatgtaccag aggttgttaa tttagtcttc
     2161 ttcaagaaag tacgctctaa aactaagttt tggcagatga ttggtcgagg aacccgtcta
     2221 tgtaaagatt tatttggacc tgagcaggat aaggaaaact tcttggtatt tgattatggg
     2281 gacaattttg attattttcg tgcagatcca agagatggag agggtcgtca cattgtttcg
     2341 ttgactcagc gtttatttaa tatcaaagtg gacttgattc gagaacttca gggactccaa
     2401 taccaagaag atcagtttgc gagagcatac cgtcagcagc ttgtctcgga acttcaaggt
     2461 cgtatagaga gcttaaatga gttggacttc agggttcgta tggttttaga tacagtttat
     2521 agctatagga aattggaaag ttggcagaat ctaactgctg ttacaagtga aaccattcaa
     2581 aaaaatctct ctctgctttt atttgatgaa gataaagaag atgagatggc gaggagattt
     2641 gatttgtggt tgcttcatat tcagttgggg caactgacag ctaaatcttc cactgttcat
     2701 atttcccaag tgatgaagac ggctagagct ctttctgcta ttggcaatat cccgcaggtt
     2761 tttgagcagg ctgaaattat caggaaagta caggagcctg aattttggaa agaagttaac
     2821 ttgtctgatt tggaaaaaat tcgtcttgct attcgagatt tattacagtt tttggataaa
     2881 acagaccgta aaccctacta tgttaacttt gaagatcgta tactctccac tgttcacgag
     2941 accacagcat ttttgcaggt caacgatctt cggtcttaca atgaaaaagt tgagcattat
     3001 ttgaaaactc atctggatga ggagtccatt tctaagctat accataataa aaagttgaca
     3061 tctgatgata tgcttgcact tgaaaaattg ctttgggaaa aattaggtag taaagcagac
     3121 taccaaagtc attatgaaaa taaggcaatt ccgagattgg ttcgtgagat tattggctta
     3181 gatagagagt ctgccaatcg tattttttct aaatttttgt cggatgagaa tcttaatgcc
     3241 aggcagattt catttgtaaa attgattgta gactacattg tagaaaatgg ttttttagag
     3301 acgaaagtgt taacgcaaga gccgtttaaa tcttatggtt ctgttcaact actcttccaa
     3361 caccaactac cagtacttcg taatattgtt caaatcattg aacttatcaa taatcgagct
     3421 ggagaagcgg cttaaattct aaagtgattg ccatgctgag actcatttaa aattaaaaag
     3481 agtagaaatt tatgctatat atgagaagtt ttattaggaa gaatgtcatc gttttcctag
     3541 aatacagtat cagttgttaa gtggttgata aatttcaaag tagatacttg taccacgatg
     3601 tttgttgatc gagttattaa caaaagagct actttgattt taaagaaata gaaaacaaaa
     3661 agccgagcaa gaattcaatt gcaggagaaa atgaaataat actcaatgaa aatcaaagag
     3721 caaactagga aactagctgc aggctgctca aaacactgtt ttgaggttgt agatggaagc
     3781 tgacgcggat tgaagagatt ttcgaagagt ataaatcttc ctaggataaa gcaaaacgca
     3841 tagtatcaag ggttttcaac acttgatact atgcgttttc tgatgttaaa gactttctac
     3901 caggtttttt aaaagcataa ttgttagttg tagtcattta ttattcttca aagaaaaatg
     3961 gtggggcgaa ttttttcagt tcttcaaagc acttttgagc agtatctgca tcttcacaga
     4021 tgataagaca gacatcatta ccacaaaggg tagcgatagc gtcagggaag ctcaaagtat
     4081 caatgataga accaaaggat tgagccagtc caggaagggt ttttagtagg acttggtgtt
     4141 gaactgggcg catccagaca agggcgtctt ccatgtagag ttcgagacgt ttttcccatt
     4201 ttgagatgga accattgtta agaacataat aagcgctatc ttcttcgcgg acttttgata
     4261 ggttcatatt tttgatgtcg cgtgagaggg ttgcctgggt tacttgaatg tcgttctcag
     4321 caagaagggc ttgcaactca gcctgtgtat gaatcttgtt ttttgtgata agagcgcgta
     4381 taagttggtg gcggtgttct gatttattca taataatgta actcctttta gcaaggtaag
     4441 gtaagcatgg actgagcgag gtcgacagtc aagtggtagt ctgtattgtc acggatggtg
     4501 atttcaaagt cagtagtata gaggactaaa cggagagtgt ctccttcttt tagcttgtaa
     4561 atagttggct gcagttcaaa ttgaacgtcc atccattcat ctgcagtaat atcctctact
     4621 aacagtaaat catttctatt ttgtaaatta aggtaacctt ttgtcacgac tcgttgtgcc
     4681 tctggtctaa atggcaattc acagagattt tccaacatgt gatagcgacc gttgtcaatg
     4741 gttctagcac ttaaaatagc tggataaggt tgtaggtatt tcttttgccc aaattctagc
     4801 agttgggcag ataagagccc cttgtttgta ctggatttga tacgaagatt gagctgagcg
     4861 cgaccgttta ggtggagatc tttagtcaca ggaaggttaa tagtaatctg attggctttc
     4921 ccttgataga gctctgtatt gaaggtttgg tatgtcttac catagcgctc aaaatcctta
     4981 tctgggtact ggttttgaat agcttgctct tcttgaccaa gtgagaaggt ttcacagttt
     5041 tcttgcccac cgaagttatc aagtgataac caagtctgtg gagctgtatt gtcctgccag
     5101 ataacagtag gaagttgaaa gtctgtttcc tgtcctagta atttcttggt caataaggca
     5161 tttatggact cacggaagtc aattgattgc caattgttca tgtaaacatg ggcaccatta
     5221 tggaaaaaga gatgcttgtg tatatgagta ggaagagcat ggaacatctg gtaaacatga
     5281 agtggtttga cattccaatc ctgagaacca tgagtaaaga caacctctgc ctttacttta
     5341 tgggcattga gcagataatt gcggtcatgc caaaactgat tgtagtcccc agtttttcgg
     5401 tctagctgag ctttcacttt ttctaagtca gcttggtgag cttcattgcc acggatatag
     5461 tcgccagcta agagattacg agaataggtt aactcagcaa gggagtcaaa gtcctcacct
     5521 ggataaccac ctgggctagt caccagaccg ttttcacggt agtagttgta ccatgatgaa
     5581 attcctgcct cggcaatgat aacttctaaa ccatcgactc ctgtagtcgc aagaccattg
     5641 gacatggtac ctagatagga aagtcctgtt gtagcaactt ttccgtttga ccaatcagcc
     5701 ttgacttgac gctggcgcgt gtgatcagta aaggcacggc aacggccgtt aagccaatcg
     5761 atgacatttt tataagcctc gatttgctgg tagtctccat tagtcatgaa acctgtcgag
     5821 tctttggtac caacacctga gacatagaga ttggcaaagc ctctcggaag gaagtagtcg
     5881 tttagtgtat agctagagtt gatgtgagtt agcttttcct cagcctctgc tataagctca
     5941 gctttacctt ggggttggac gagatttagt tgaggtttct ctagctcaat cttgtgagga
     6001 agtttaacct caagctcgcc ctccatcttg tagagagcct tgtcactagc cttgtcattg
     6061 gttccctgat gataagggct ggctgtcatg atggcaggga tttttccatc aaaacgaggg
     6121 cgaataatgc taacctttac taggtctgat agcccttttt ggtcagtatc gacacgagac
     6181 tcaacgtaaa cgacttcacg aatgacatcc tggttagaaa aagtagccaa actcttgccg
     6241 ttaaagtagt ggtagtcatt atcctccgga ataagaccat cactaacaag ttggtcgata
     6301 agagtatttc cttttttggt gcgagtattg agtaactgat agagattttc aatcaagtca
     6361 ccatatataa tgggaaatcc agtttcttta cgaaaaacgt cactatcttc gaagtcaacc
     6421 aaataagcaa agcctaaaag ttgaaaagca acagtataaa aaatatctgc tgtcagttca
     6481 tcttctgatt gaaaaaatgt cagcaggtct gtttttttat cagctgctag gatagaaagt
     6541 gggtagttgg tgtcttgata agtgaaaaag aaacgacgta aaaaggtttc aagtgagtct
     6601 ttgtgattgg ctgtattttg taaatcaaag ccacattttt ttagttcaga taagacattt
     6661 tcttttggaa aattgatata actatattga ttaaaacgca tagaacctcc atatagaatg
     6721 acagttaagg ttattatatc aaaaaaaaag cagaaaggga attgttaact tcaaaaggaa
     6781 ataatccaat aaaaatgaat aaagtactaa attcaatata gagaacagag taacaataag
     6841 aataaataga tagggtataa aagttctagg agatttatat tatatgcttt ctatttttat
     6901 atacaatata gtataaatat aaaaatgatg acaaaaatac aaatgaatag aaaataaatt
     6961 agtaagctga tgaaattttt ctcaagagaa gccatttata ggtgaaaatg gtataatata
     7021 gtgagaagga tagaggagaa gtgtaaattg atcgcacaac tagatacaaa aacagtctat
     7081 agttttatgg aaagcgtcat ttcgatcgaa aagtatgtga gagcagctaa agaatacggc
     7141 tacactcatt tggctatgat ggatattgac aatctttatg gcgctttcga ctttctagag
     7201 attacaaaaa aatacggcat tcatcctttg atagggcttg aaatgacagt gtttgtagat
     7261 gatcaggaag tgaatttgcg ctttttagct ctatctagtg tgggctatca gcagttgatg
     7321 aagctttcga cagccaagat gcagggggag aaaacttggt cagtcctgtc ccagtacctg
     7381 gaggatatcg cggtcattgt gccttatttt gatagagttg agtcgttaga actaggctgt
     7441 gattactata taggggttta tccagaaaca ctagcaagcg aatttcatca tcctatctta
     7501 cctctttatc gggtcaacgc ttttgaaagc agggatagag aagttcttca agttttaaca
     7561 gcgattaaag aaaatctacc gctcagagaa gttcccttgc gttcgagaca agatgtcttt
     7621 atatcagcaa gttctttaga gaaactattc caagagtgtt ttccgcaagc tttggacaat
     7681 ttagaaaagc ttatttcagg catttcttac gacttggata ctagtctgaa actgcctcgt
     7741 tttaatccag ctagaccagc agtagaggag ttgagagagc gtgctgaact ggggcttgtt
     7801 cagaaggggt tgactagtaa agaatatcaa gatagactag accaagaatt gtctgttatt
     7861 catgatatgg gctttgatga ttatttcttg gttgtttggg atttgttgcg ttttggacga
     7921 tcgaatggct attatatggg aatgggaagg ggttctgcag taggcagttt ggtttcttat
     7981 gccttagaca tcacggggat tgacccagta gagaaaaatc tgatttttga acgctttctt
     8041 aatcgtgaac gctataccat gcctgatatt gatattgata tcccagatat ttatcgtcca
     8101 gattttatca gatatgttgg taataaatat ggtagtaaac atgcggcaca aatcgttact
     8161 ttttcaacct ttggagccaa gcaagctctt cgagatgtct tgaaacgctt tggtgtgcca
     8221 gagtatgaat tatctgcaat tactaagaaa atcagttttc gtgacaatct taagtcggcc
     8281 tatgagggca atctccagtt tcgtcagcaa atcaatagta agttagaata ccaaaaagct
     8341 tttgagattg cttgcaagat agagggctat ccaagacaaa cctctgtcca tgcggctggt
     8401 gttgtaatta gtgaccaaga tttaaccaac tatattcctc taaagtatgg tgatgaaatt
     8461 ccactgactc agtatgatgc tcatggagtt gaggctagcg gacttttgaa gatggacttt
     8521 ctgggactac gaaatttgac ctttgtccag aagatgcaag agttgcttgc tgaaatagaa
     8581 ggtattcacc ttaaaattga agaaatagat ttggaagaca aagaaacgtt agctttattt
     8641 gcctctggaa atacaaaagg tatctttcaa tttgagcaac caggtgccat tcgtctgctt
     8701 aagcgtgtgc aaccagtctg ttttgaagat gtcgtcgcga ctacttctct aaatcgaccg
     8761 ggtgctagtg actatatcaa taattttgtg gcaagaaagc atgggcagga agaagtgact
     8821 gttctggatc cagtactgga ggatattttg gctccaacct acggcataat gctctatcag
     8881 gagcaggtta tgcaggttgc ccagcgattt gccggattta gtcttgggaa agccgatatt
     8941 ttgcgtcggg ctatggggaa aaaggatgcc tctgccatgc atgagatgag ggcttccttt
     9001 attcaaggtt cattagaagc tggtcatact gtggaaaaag cagagcaggt ctttgatgtt
     9061 atggagaagt ttgcaggtta tggttttaac aggtcacacg cctatgccta ctcagccttg
     9121 gccttccagt tggcttattt taaaacacat tatccagcca ttttttatca ggtcatgtta
     9181 aattcttcca acagtgatta cttaatagat gcacttgaag caggttttga agtagcctct
     9241 ctatccatca acaccattcc ctatcacgat aaaattgcca acaaggccat ctatctaggt
     9301 ttgaaatcca ttaaaggagt cagtaatgat ttagctctct ggattattga aaatagacct
     9361 tattctaaca ttgaagattt tatagctaaa ttacctgaga attatctgaa acttcctctg
     9421 ctagaacctt tggtaaaagt tggtcttttc gattcatttg aaaaaaatcg tcaaaaagta
     9481 tttaataatt tagctaatct atttgaattt gtgaaagagt tgggaagttt gtttggagat
     9541 gctatttata gttggcagga atcggaagat tggacggaac aagaaaaatt ttatatggaa
     9601 caagagcttt tagggatagg tgtcagcaaa catccactac aagctattgc aagtaaggct
     9661 atttacccga ttaccccaat cggaaatttg tcagaaaata gctatgctat tatcttggtt
     9721 gaagttcaga aaataaaagt gattcgtacc aaaaagggtg aaaatatggc cttcttacag
     9781 gcagatgata gtaagaaaaa attggatgtc actctctttt cagacttata tcgtcaggtt
     9841 ggacaggaaa taaaagaggg agccttctac tatgtaaaag gaaaaataca atcacgtgat
     9901 ggccgtctgc aaatgattgc acaagaaata agagaagcag ttgctgaacg cttttggata
     9961 caggtgaaaa atcatgaatc ggatcaagaa atttcacgca ttttagaaca atttaaaggc
    10021 ccaatcccag tcatcatccg gtatgaagag gaacagaaaa ccatcgtttc tccccatcat
    10081 tttgtagcta aatccaatga attagaggag aaattgaatg aaatcgttat gaaaacgatt
    10141 tatcgctaaa aatacggaaa atagaagaat tttaaaatca aatgtgatat aatcaagaag
    10201 aatgttaaaa gaaaaaggag cataaaccaa tatgaaacgt attgctgttt tgactagtgg
    10261 cggagacgca cctggtatga acgctgccat ccgtgcagtt gttcgtcaag caatttcaga
    10321 aggaatggaa gtgtttggta tctatgacgg atatgctggt atggttgccg gtgaaattca
    10381 tcccctagat gcagcttcag taggggacat catttctcgt ggtggtactt ttcttcactc
    10441 agctcgttat ccagagttcg ctcaacttga agggcaactt aaagggattg agcaattgaa
    10501 aaaacacgga attgaaggtg tagttgttat cggtggtgac ggatcttacc acggcgctat
    10561 gcgtttgact gaacatggct tcccagctat tggtcttcca ggtacaatcg ataacgatat
    10621 cgttggtact gactttacaa tcggttttga cacagcggtt actactgcca tggacgctat
    10681 cgataagatt cgtgatacat catcaagtca ccgtcgtact tttgtaatcg aagttatggg
    10741 acgtaacgct ggtgatatcg ctctttgggc tggtattgca actggtgctg atgaaatcat
    10801 catccctgaa gcaggcttca agatggaaga tatcgtagca agcatcaaag ctggttatga
    10861 atgtggtaaa aaacacaata tcatcgtctt agctgaaggt gtgatgtcag cggctgaatt
    10921 tggtcaaaaa cttaaagaag ctggagatac aagcgacctt cgtgtaacag aacttggaca
    10981 tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt ttggcgtcac gtatgggtgc
    11041 acatgctgtt aaacttctta aagaaggtat cggtggtgtt gcggttggta ttcgtaacga
    11101 aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa ggggcattgt ttagccttac
    11161 tgcagaaggt aagattgtgg ttaacaaccc gcacaaagcc gatattgagc tatctagctt
    11221 gaataagagc ttgtcataat ttacaattta gttaataaga ccaaaaaggt catatatata
    11281 aaggagtcac aaaaatcatg aacaaacgtg taaagatcgt tgcaactttg ggtcctgcgg
    11341 tagaaatccg tggtggtaaa aaattcggtg aggacggata ctggggtgaa aaacttgatg
    11401 ttgaagcttc agctaaaaac attgctaaat tgattgaagc tggtgctaac acattccgat
    11461 tcaacttctc acacggcgac caccaagaac aaggtgagcg tatggcaact gttaaacttg
    11521 cggaaaaaat tgcaggtaaa aaagttggtt tccttcttga tacaaaagga cctgaaatcc
    11581 gtacagaatt gttcgaaggt gaagctaaag aatattcata caaaactggt gaaaaaattc
    11641 gtgttgcaac taaacaagga atcaaatcaa ctcgtgaagt gattgcgttg aacgttgctg
    11701 gtgctcttga tatctatgat gatgttgaag ttggtcgtca agttttggtt gacgatggta
    11761 aacttggtct tcgtgtggtt gctaaagatg atgcaactcg tgaatttgaa gttgaagttg
    11821 aaaacgatgg tatcatcgct aaacaaaaag gtgtgaacat ccctaacact aaaattcctt
    11881 tcccagctct tgctgaacgc gataacgacg atatccgttt cggtcttgaa caaggtatca
    11941 acttcatcgc aatttcattc gtacgtactg caaaagatgt gaacgaagtt cgtgcaatct
    12001 gtgaagaaac tggaaacgga catgttcaat tgttcgctaa aatcgaaaac caacaaggta
    12061 tcgataactt agatgaaatc atcgaagcag ctgatggtat tatgattgct cgtggtgata
    12121 tgggtatcga agtaccgttc gaaatggttc cagtttatca aaaaatgatt atcaagaaag
    12181 tcaatgctgc aggtaaagtt gttatcactg caacaaacat gcttgaaaca atgactgaaa
    12241 aaccacgtgc aactcgttca gaagtatcag acgtattcaa cgctgttatc gacggaactg
    12301 acgctacaat gttgtcaggc gagtctgcaa acggtaaata cccactcgag tcagtaacta
    12361 caatggctac aatcgacaag aacgctcaag ctcttcttaa tgaatacgga cgtcttgatt
    12421 cagattcatt tgagcgtaac tctaagacag aagtaatggc ttctgctgtt aaagatgcta
    12481 ctagctcaat ggatatcaaa ttggttgtaa ctcttactaa gacaggtcat actgcacgtt
    12541 tgatttctaa ataccgtcca aatgctgaca tcttagcatt gacatttgac gaattgacag
    12601 aacgtggctt gatgttgaac tggggtgtta tcccaatgtt gacagatgct ccatcttcaa
    12661 ctgacgatat gttcgaaatc gctgaacgta aagcggtaga agcaggtctc gttgagtcag
    12721 gcgatgatat cgttatcgtt gctggtgtgc cagtaggaga agctgttcgc acaaacacaa
    12781 tgcgtatccg cacagtacgt taagaaaaat ataaaaacct atcatatcca gctttctagc
    12841 ctgtatgatg ggctcttttt ttgtccaggg ggcaaaaagg gggcaaaact ttcataatgg
    12901 attgatactc ttcgaaaatc aaattcaaac cacgtcagcg tcgccttacc gtatatatgt
    12961 tactgacttc gtcagttcta tctacaacct caaagcagtg ctttgagcaa cctgcggcta
    13021 gcttcctagt ttgctctttg attttcattg agtatgaaat aagatatgca taaattgatt
    13081 agaaagtcaa atgaatttct acaaatgttt tagcaatcgc aatctacttg tctagattcg
    13141 atctgatata ttttcgattt aatgatatgg tatttaaaac ctccaaagta gcttgctcca
    13201 ttcttttacc tacgtgagtc tagatgttat ttactgtttt agcgtttttg tgttctactc
    13261 taaccattat agcattcttc tcagctagtg tactaaggag tgtgtgcctg aaaatatggg
    13321 aactaagggg ctggtttatc ggtttctcta gtttagtatt tgccttttgc aaagtgatct
    13381 taaatgcctt tctctaaatt tacatatcac tattgtttaa caaaatctaa tctattttag
    13441 gtcacttatt ctttttttga aatgtagaat gaactttttc aaagtttttc gaatctttta
    13501 aaatctgttt gttttatatc gccattctcc ccctttttta attctcccta tatagcctga
    13561 caacttttcc gatggtacga atatggttgc tttcgtctag gtggatgtcg gagtattcgg
    13621 gattgagttt ttttgaggta gccttggcgg agtttcttga catagttagt gccgtctact
    13681 tggaagatgc cgatggtatt atagtcaatc tgtggggtat tcttgataaa taggtagtcg
    13741 ctgtttctta tctttggctc catggacttg ctgacgacat aagcgattgg gtcgtagtcg
    13801 tctgggataa tggaaactcc ataactaaat cgtcgtcctg catcaagcgg ctacctgcag
    13861 agataaacta cctaacacga gagtaagtag tctgtctgta gtcgtccagt ctgatgattt
    13921 ttacgatact tcgtttttct gatcatacag ttgcctctcg gcataggtca gaactttacc
    13981 ttgtctgggt ggtgcccgtt ggtcgtagat agattggata tcgctaggag aatccttttg
    14041 aactggagga aagagggcat caatcaagct actgaatact ttaactaagt caaatatagt
    14101 atttttctta gtagacctaa cccttttttc ataatttcta atggtgtttt tacttatacc
    14161 tatcttagta cccaattctt attgagtcca accattacta gtctatattg ttttatagtt
    14221 gattgagttt ggaatagtac gctgtagctg ctaaaacatt tctaggaatt aatttgcctt
    14281 tcctaataga gttgttcata tcttatttca atctactata tttttcacct ctaacaatcg
    14341 caatctcttc tttatccatg aacgaaaacg ctttctatct ttgtaagtaa agcatcacac
    14401 gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa aaaatttgtt gacagagcac
    14461 gaaacgccta ctataattaa atcaagttta aggaaataac aaaaaaacaa atcggaggga
    14521 aagacgatga acacattaaa tgagaaagta atcaatatct gtaaagcagt agttaaagaa
    14581 actttaatcc aagacattta ggagaaagaa atgaagaaat caagaaaatt agccacatta
    14641 ggaatctgtt cagccttgtt tttaggcttg gctgcttgtc aacaacaaca tgctacttct
    14701 gaggggacga atcaaaggca aagcagttca gcgaaagttc catggaaagc ttcatacacc
    14761 aacctaaaca accaggtaag tacagaagag gtcaaatctc tcttatcagc tcacttggat
    14821 ccaaatagtg ttgatgcatt ttttaatctc gttaatgact ataataccat tgtcggctca
    14881 actggcttat caggagattt cacttccttt actcacaccg aatacgatgt tgagaaaatc
    14941 agtcatctct ggaatcaaaa gaagggcgat ttcgttggga ccaactgccg tatcaatagt
    15001 tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg aaaagaatga ccagttgctt
    15061 ttcctagata atgatgcgat tgataaagga aaggtctttg attcacaaga taaggaagag
    15121 tttgatattc tattttcgag agttccaact gaggcaacta cagatgtcaa ggttcacgct
    15181 gaaaagatgg aaacattctt ctcacaattt caattcaatg aaaaagctcg aatgctgtct
    15241 gtagtcttgc acgacaatct ggatggcgag tatctgtttg taggccacgt tggggtctta
    15301 gtacctgctg atgacggttt cttatttgta gagaaattga ctttcgaaga gccctaccaa
    15361 gcgattaaat ttgctagtaa ggaagattgc tacaagtatt taggcaccaa gtatgcggat
    15421 tatacaggcg agggactggc taagcctttt atcatggata atgataagtg ggttaaactt
    15481 taatagtgtt atgatatgac agtaatctta ggatttttat ttctattttt gatgagttgg
    15541 tgggttattt caatcgttaa tagccagaaa ccacgcaaaa atgaaacatt tattggctat
    15601 atccaaagat acgatattga tgggaatggc tatcaatcat taccgtgtcc tcagaactga
    15661 gaggagttct tgaaatcgta aaactacttt gaacaagagt tacttcaacc cattggctcc
    15721 gacggattaa gttgctttcg tgaacaccaa aatcagccgc aatttcttca taagtgcggt
    15781 attctcgcac atattgaaga gtggccataa gaaggtcttc taggcttaat ttaggttttc
    15841 gtcccccttt tgtgtgttta agttgataag cagtttttaa tacagctaac atctcttcaa
    15901 aagtggtgcg ctgaacacca acaagacatt taaatcgtgc atcagttagt tgcttacttg
    15961 cttcataatt catagaacta ctataccata ttttgtttcg caggaagtct aatgctaaaa
    16021 tcttatcagt gagaagattt cttaaagttt attctttgta gtttcatctg tattagtaca
    16081 taatttaact tagagaaaat aggaggacta atcgaatgga attaaaagat tttacagaaa
    16141 aagaacagga aatgattaag aaaaggctta caatgtctaa tattagtgac aaagaaacta
    16201 ctgagaagat tctggcgcta gtaccacaag acttgattaa gcgaatcccg ttttttgtca
    16261 gaaaacatgc tacaacacgt acgattaaac gcatttcaat tgaacaccct gaactctacg
    16321 ctgtagctca aacaagtggt gagattccag aaaaaaaacg cgaagaattg cgtcagatta
    16381 tcacaactat ctttgaacaa aagatgaata agcatagtat taagtagaga atgaaaaaat
    16441 attttattgg cggtttggga agcaatgcct atcatagcaa ggattttctt taagaactag
    16501 attcgcaggt ctattttcta aatccatatg aaaagcatct tcgagatgaa acagaattga
    16561 aatcatggtt taaaaatgag attgtagagg aagaatctat ctgtctgata ggtcattcta
    16621 ttggaggcga tttagctcgt tatttcgcat cggaatttga agaagtaaag aaactgattc
    16681 ttttggatgg tggctatcta gatttagata agattttacc tttggataca gagttagagg
    16741 aaactaaaaa ttatatcaaa tctcaaatta tttcggactt agatgttctt acttctaaag
    16801 aaaaatctga agcaaagcat tggtcagaaa atatggagaa agctgtaaga cagtcctatc
    16861 actggaatgt tgagtataat agatatgagt tggctataaa ttatgaaaat atagaagcga
    16921 tactccgcct acggagaaaa atacaagctt ttaagagaga agtgggagat accttgttta
    16981 ttagtcctcg ctatcctaat gaagctacat ggagagagga agccctaaaa gaattgccag
    17041 actattttga tactattttt ttagaaaact ttggccatga gctttatact caagcaccta
    17101 aagaaatcgc tagtctaatg aatgagtggc tcgcttattt tctatgagct acttgtattg
    17161 ctggaaatgg tataagatga aaatctacaa gtcactaatt ttcatataac caaaaaacga
    17221 acaggctctt tttctgttat tcagaagaga acttgttcgt ttttatcttg attttcttaa
    17281 aaagctctat atacataggg attttctggt ttatcgactt tggtaaagct gtcgatttcc
    17341 aaggttggaa ggttttgttt gagttcttta tggtagtgat tgaccagttt tcctttggct
    17401 gaaatccaag tgttgttttc atactgccgg gtattgccct tggtcagcaa tccatagaca
    17461 ccagaatctg cgatacagtg gataatgccg aatcggaaca gaaattgact attggcatga
    17521 ctggggtcgt tatagacaaa gcctgtaaac tggattgtct tgccctcaaa ctcatctgga
    17581 tagtcgtaga tagcctccat gacttccata tagttttcat tagtgatctg aatgctgtct
    17641 tgggataagt atttatccgc cgccgttcgc atttcctttt cataggctga ttttgaaaaa
    17701 taagaactag tatctggttt caaatattgg cttgtcgtcc cttcgcttgt ctgaatagcc
    17761 agattcgttc cttccgatag agggaaatga tagcctttag ctgaaacagt ctgagaatcc
    17821 aagctaacag ttgggaaagt taagccgatg acaagtggaa tagccagaag agaaatactc
    17881 gtcatcttgg ccaatcggct gttcagatga ctgtgggttt tgacttgctt catccagata
    17941 tacaattgaa cgatagccaa gataaaagaa agcaccatgg agatataggc cagataggaa
    18001 tagtgcatgt tgatgtactg gtttagtttg cccgacagat ggaggtaaat agtcagttca
    18061 aaatagccag ctaaaactaa aaatcgaatc ataggatgac tccaatcaag agagaataga
    18121 ctaagacgac aagagttaca attgttatga agtgactgat aaatcgtgct ttcaagtaat
    18181 ttttcatcat gagaatattt ttgatatcca gcattggacc aatgacgaga aaggccagaa
    18241 ctggtgccaa accgaaactc gagagaagag aagcacctat aaaggcatcc gcctcactac
    18301 agagcgaaag aagaaaggct aaaatcatca agagcaggat ggcaaaaaga ggggtcgcac
    18361 tgatagaggt cagaatccga gtcggaacgt agacctgtat tatagaagca aagaggcagc
    18421 caaataccaa ataacgcccc gtatcaaaaa attcatcaat ggcctgcaca aagacttgaa
    18481 aaactttttt tgcagaactc aagtaagaaa aatcatgctc atgacaagcc agacgatttt
    18541 ctttctgaat cggttcttgc cagaaaaatc ctagaaaaat tcctagtatt acagccacaa
    18601 gaatggaacc cagagctcgt aatagggcga catggaagga gttgccaaag gcagaatagg
    18661 tcgcaaaaag aacaatggga ttgataacag gtgctgtcac aagaaaagga acggccgtgt
    18721 aacttggaac ctttttttcc agaaaacgat tgatgatggg gacgattcca cattcacaag
    18781 aagggaaaag tataccgaca aaggtcccaa aaaagattct cccccaacga tttcgaggga
    18841 gaaaatgata aaccttgtca ggtgtgatat aaacttcaat cagccctgag acaatgcttc
    18901 ctatcagaac gaagggaagg gcttcaatga taatggagag aaaaatggct ccagtttgaa
    18961 gtacactaga tgggagttgt tgaaaagcag tcatctagtt tttcttttct gctttgactt
    19021 ttttctcttt gtcatcaggg aaagtaactt gtttcaaatc agggagttta gcaaactctt
    19081 caaacatctt gtccaagtcg tctcgttttg taaatttgac agccataggt gtacctcgat
    19141 tctaaattct acctctatta tactattatt ttgcggaaaa gggagttttt taatttgttt
    19201 tcgatagttg atactcttcg aaaatctctt caaaccacgt cagcttcgcc ttaccgtagt
    19261 a
//
LOCUS       DAVMMR010000037        19086 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMMR010000037 DAVMMR010000000
VERSION     DAVMMR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.037"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..109
                     /locus_tag="VYS74_001324"
     CDS             <1..109
                     /locus_tag="VYS74_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3789360.1"
                     /translation="SVTILEYEDGQFRVEVVGDRSYRELGREKMEEGSI"
     gene            complement(212..1857)
                     /locus_tag="VYS74_001325"
                     /pseudo
     CDS             complement(212..1857)
                     /locus_tag="VYS74_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(1847..2922)
                     /locus_tag="VYS74_001326"
                     /pseudo
     CDS             complement(1847..2922)
                     /locus_tag="VYS74_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2935..4056)
                     /locus_tag="VYS74_001327"
                     /pseudo
     CDS             complement(2935..4056)
                     /locus_tag="VYS74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(4254..4451)
                     /locus_tag="VYS74_001328"
     CDS             complement(4254..4451)
                     /locus_tag="VYS74_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3789361.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(4526..5302)
                     /locus_tag="VYS74_001329"
     CDS             complement(4526..5302)
                     /locus_tag="VYS74_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV3789362.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGKEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            5424..6170
                     /locus_tag="VYS74_001330"
     CDS             5424..6170
                     /locus_tag="VYS74_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3789363.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            6183..7163
                     /locus_tag="VYS74_001331"
     CDS             6183..7163
                     /locus_tag="VYS74_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV3789364.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            7358..7678
                     /locus_tag="VYS74_001332"
     CDS             7358..7678
                     /locus_tag="VYS74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3789365.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            7724..8032
                     /locus_tag="VYS74_001333"
     CDS             7724..8032
                     /locus_tag="VYS74_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3789366.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            8049..9347
                     /locus_tag="VYS74_001334"
     CDS             8049..9347
                     /locus_tag="VYS74_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3789367.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGTIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            9498..11936
                     /locus_tag="VYS74_001335"
     CDS             9498..11936
                     /locus_tag="VYS74_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV3789368.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            11955..12623
                     /locus_tag="VYS74_001336"
     CDS             11955..12623
                     /locus_tag="VYS74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV3789369.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            12641..13729
                     /locus_tag="VYS74_001337"
     CDS             12641..13729
                     /locus_tag="VYS74_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV3789370.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            14184..16685
                     /locus_tag="VYS74_001338"
     CDS             14184..16685
                     /locus_tag="VYS74_001338"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV3789371.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            16824..17666
                     /locus_tag="VYS74_001339"
     CDS             16824..17666
                     /locus_tag="VYS74_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789372.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIYFIWLLIEGLKY
                     IRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQN
                     SDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKL
                     EISR"
     gene            17722..18417
                     /locus_tag="VYS74_001340"
     CDS             17722..18417
                     /locus_tag="VYS74_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789373.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            18429..18845
                     /locus_tag="VYS74_001341"
     CDS             18429..18845
                     /locus_tag="VYS74_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789374.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT         6095 a         3002 c         3944 g         6045 t
ORIGIN      
        1 tagcgtgacg attcttgaat atgaggacgg tcagtttagg gttgaagttg tcggtgaccg
       61 tagttaccga gagctaggac gtgagaagat ggaagaaggc tctatttaat cagtctagac
      121 ttgcttgcca tgagctaggg atttgataag aatatcaaga taagaaaaaa cagccgaggg
      181 tactcctttc ggctgttttt gatgtggaaa actaaagtgt aatgctattg cttttagaga
      241 ttttcataaa caagagcaag gaacctactg ttagaacagt caggatagtt gacaaggctg
      301 cggctacacc gtaatttcct ctgagaacct ctgtataaat agctacagtc attgttcttg
      361 ttttgacatt gtagaggagg atagaagtag agagttttga aatcattgtg acccaagata
      421 agatggctcc agaaatgata ccagatagca tcattggagt tgtaatctta gcaaaggtat
      481 tgagacgact acttcctaag ctttcagcag cttcttcaat acttggtgct atttgttgta
      541 agctagcaac agatgagcga atagtatacg gtaatcttct gacagataga gacataatca
      601 agatgaaagc agtccctgta atcataagaa atccacttcc aaatagacca gtattgaagg
      661 aagaaatgaa ggcaatccct agaacggttc ctggtacaat ataaggtacc atactgaggc
      721 tgtcaattaa gtttgtaaac aaattccgtt ttctaacggc taggtaggag ataaatgtcg
      781 caaatagaac aactagaact aaggcaatca aagggatacg aatggtattg aaaatagcag
      841 atcccatacg attgaaagct agcttgtaac tgtttggaga ataaccttta acagatacca
      901 tacctgatgt ttttaggaaa gaggtataaa ttaagtagat ttgaggtaaa acagagataa
      961 agataattcc gtagactgtt gcataaatgg cagctatttt tccttttgta gtttttttag
     1021 gctcaattgg atggagcaga ttcatgctga aactgtagcg gtttgcaatg tgtttttgga
     1081 taaggaaaat tgccaaggca atgataatcg ccataattgc aaaagcagaa tttcctccaa
     1141 cctcgctaat aaattgggta taaatcagga cagggaaagt ccgatatcct tcgccaatca
     1201 acataggcgt tccaaagtct gagaatgctc tcataaatac aagcaaggat ctgctagtaa
     1261 ggttggaact aggagaggta aaacaaccgt tacgatacgc ttaaatccga aggaccccat
     1321 gctttcagct gcttcaagta gagaattgtc aatactgttc attgttccag caacatatag
     1381 aaataccagt gggaatagtt gcagtgtaaa gacaagtaca attcctttga atccataaat
     1441 atcgatagct ggaagatgaa gggcatttgt caaaaattta gtgatgacct catttcgtcc
     1501 cagcaagaga attcaggagt aggctcctac gaaaggagct gacatggaag caatgataat
     1561 caatatttgt agaaatttct tccccttgaa gtcatacata gagaagagat aagctaatag
     1621 ggttcctaca actaaggaag tgatagtagc ggtaatggaa accttgaaac tgttgactag
     1681 tgtctcagag tagtaggctt tactaaagaa agtgacaaaa ttagctagtg agaattgtcc
     1741 ttcatgtata agtgcttgct tgagcacggt aacgatagga taaacgagaa agataggata
     1801 ggtaagaaag aggaagaaag aggaagctgt ccaaatattt agttttttac attccatggt
     1861 tgactccttt tatcagattt tgggaaccat ctgcagaaaa gctgtttaat ttttgcgtat
     1921 tgattcgtag acgaatacga ttgccttttt gtagatcttc ttcaaaagtt gattcttcac
     1981 taacttgaat ttttgaggca aaacctgtct caatgaaata ctccgtattt agtccaagat
     2041 agacgctatc gctaatagtt ccttcaatat ctccagattc atctttgata aactcttcgg
     2101 gacgaatgct tacatgaata gcttgctcct caacctgatc aagagctagc attcgaagag
     2161 catagccatc tgaaaagacg atataagcgc cgtcgctccg tttttcaaga ttggcaggga
     2221 taatatttgt gcgtccgata aaggttgcca caaactcatt agctggttta tgatagagtt
     2281 cttttggtcg gccgatttgt tggatcaccc catctttcat aacagcaatt tggtctgaaa
     2341 tagccatggc ttcttcttgg tcgtgggtta cataaacagt tgtaattccc acttcgtgtt
     2401 ggatttctcg gatggcttga cgtatatcca agcgaagttt ggcctccaga ttactaagtg
     2461 gctcgtccat gaggagaaca cttggattaa ccgctaaggc gcatgccaag gtgacacgtt
     2521 gttgttgtcc accactgagt ttatcgggct ttcgatccgc atattgagca atttgtatga
     2581 gttcaagata cttgttggtc tgttgaatca attcttcttt tggaaccttc ttttgcataa
     2641 gaccaaaagc aacgttgtct cggacagtca aatgtgggaa aatagcgtag ttttggaaaa
     2701 ccatcccgat attgcgtttg ctgggttcca tattattgat ttttgtatca tcgaagtaaa
     2761 attctccatc tttgatactg ttgaaacctg caatcatacg aagaagggtc gttttcccac
     2821 atcctgaagc tccaagaatt ttggaattgt aatgttcaaa ttctcaataa cagggacatc
     2881 gtggtagatt tttttggcgt taataatttt gatctcactc atagtgaacc tcttttactg
     2941 tttagattgg atatctgtaa agacttcgtt gtatttctta acgatatctg atttattctt
     3001 gatgacataa tcataatctt cagtgagtgt tttgattttg tcaattggtt tcatgttttc
     3061 gcttgtttta gcatttttac gaacaggacg gttagtagtg gttgtaccaa gtgtatcttg
     3121 tacttcttga gagataataa aatcgataaa tttcttggca ttttccatat ttttagattt
     3181 tttaacgata gcagcactag caggtaggaa gacggttcct tcttttggat agactacctt
     3241 aatgttagct ccgtcattta agagtttaac tgctggatct tcataagaga gaccaacagt
     3301 catttctcca tcagcgactg ctttatagac accagatgaa cttaaaccga ttttatagta
     3361 tattgaaact agaatagtac acatctactt ctaaaatatt gttagaaatc gatttgactg
     3421 tcctgattga tttgtcctgt tcttatttca ttttactata ccatcaataa gtgtgaaaag
     3481 atcttttaca taagaccaag ccttatcatc tttgtaacca ccttgagctt gtagcatatt
     3541 tgttaattga gcaaaggcgc tagaagagtt tgctgggtcg gcagttgcga tttttccttt
     3601 tagttcaggt ttgagaagat cgttatatcc ttcgatgttc atgcctttag ttaaatcagg
     3661 gttgacgatt aaaacactac catctagtgt ataaggagta gagtagccag ttgtgttttg
     3721 atattctttg ataacattat cattttcttt tgaagtatag ttttcaaaga gttctccgtg
     3781 ggtagcatat tgtgtataag aaccaccaaa gataacatca gctacaggaa cttctttttc
     3841 tgactctagt tttttgaaaa gttctccagt accagcttga atcagttcta ctttgatacc
     3901 atatttttct tcaaaggcag gaatagttgc tccaattaag tcctctgagt ttggtcaata
     3961 aacgactagc gaaccaccgt ctcctttatc agatgaactg tcatcggcag attcattaga
     4021 agaacaagca gcataataca tccatttctt tttcatgatg ggatatctcc gttgtgttat
     4081 ttaagtttat tttaaaacct acaattctat tctatagtat attgaatcta taacagtaca
     4141 ctttaactgc taaaatattt ctataaatta atttgacttt cctgatagag atgttcacat
     4201 cttatttcaa ttcactatat tagagtaaaa ttctctacaa aaagaagaat agcctatttt
     4261 actattcttc cgagtgattt caatttcttt ggggaaatat ggagatactt tttaaatact
     4321 ttggcaaaat atttatagtc tgaaaatcca acttggtcag agatagcgct taaaggggag
     4381 ttgggagatt cagccatctt atctatagct tgtttcaggc gatattgtaa gatatattca
     4441 tttagagtca tggggaagtc ttctttgata accttatata gatgcttgtt atctgcaaat
     4501 agctagagca tttcaaggaa atgaactaga aataacaatt aatatggtgg ttcagaaata
     4561 ccttttgata atcaataaga tcttcaggat gaagtgcgtt gactccatcc atttcatatt
     4621 tccgatttaa taaacgatat ttgttttcac caaattgatg aaaagggagt agttgaactt
     4681 ggtcgatatt taatgagtta aatagagtag cgaatttttc tgcatcctct aaactattgt
     4741 taaaattagg aataactggg attcttaaaa cgatagtttt attttgagaa aaagcataat
     4801 gaatgttttt aataatcatt tgattaaaaa ccccagtcac ttttttatgt tttatagaat
     4861 tataatgttt taggtctgtg tagataaaat ccacatattg aattaaatca ataaattttt
     4921 catgatcaac aaaggcagta gtttcaatgg cagtgtgtat gtgatgttct ttagctgatt
     4981 ttaagatggc tttagcaaat tcaaactgag caaatatttc acctcctgat aaagttaaac
     5041 ctccaccgga ttcttcgtaa aattctttgt cttttaatac ctctgtaata atttcttcta
     5101 cagtcttttc tttaccgact aaggtgaatt tctctcgttg agcatctttc attttttcag
     5161 gtttcattct ttgagattca ggattagaac accatggaca gcgcagagga catcctttta
     5221 aaaaaacagt tgtacgaata cccgggccgt catgaattga aaagtgttga atattaaaaa
     5281 taattccttt agatatttcc ataagagttc ctcctcttca taagtctatc ctatcacaaa
     5341 aacgaaagaa aaacaattac gaacgaaagg ttaatttgtt gttttttgct ttttattttg
     5401 gtagaataaa acggaggtgt aaaatgaaaa gattggaaca aattattaaa ttagtatcag
     5461 aatatgaaaa gatcgacgtt aatacattat cggaaaaatt aaatgtatcg aaagtaacga
     5521 ttagaaaaga tttagataaa ttagagtcaa aaggtttatt acacagagag catggatatg
     5581 ctgtattaaa tagtggagat gacttaaatg tacgtttgtc aattaattat gaaattaaga
     5641 gaaaaattgt tcaggaagca gtaaaattgg tgtcagataa tgaaacaata atgatagaat
     5701 ctggatcgac ctgtgcttta cttgctgagg aaatttgcaa gcaaaaaaga aatgttacga
     5761 ttgtaacaaa ttcatttttt atagcaaatt ttgtgagagc ttatgattca tgtcgtatta
     5821 ttgttcttgg tggagagttt cagaaggatt cacaggttac tgtgggacct ttattaaaag
     5881 aaatgataca gacttttcat gtgcatcaag cttttgttgg gacagatggt tacgataaag
     5941 agatgggctt tacaggaaaa gatttaatgc gcagtgaggt agttcaatat atttcagcag
     6001 cgtcggataa agtcattgta ctaactgact caagtaaatt tgataaaagg ggtacagtaa
     6061 gaagatttgc tttaagtcaa gtctatgaag taataacaga cgaaaaactt tctaaacaaa
     6121 atatagctac attagaaaat gctgggataa tggttaaggt agtttcgtaa gaggttaagt
     6181 gtatgaatca agataggaat aaactgcttt ctaaaattgc ttatctgtat tatattgaaa
     6241 acttaaatca gtcacaaata gcagcaaaat taggaattta tagaacctct attagtagaa
     6301 tgttaacaga agcaaggaat gcaggaattg ttaaaattga aatagagaat tttgatacca
     6361 atatgtttaa gttggaaaat tatgtaaaag aaaaatatgg tctggaaagt ttagaaatta
     6421 ttccaaatga atttgatgat aatccaacaa ttttatctga aagaatttct caagttgcag
     6481 caggtgtcct taggaatcta attgatgata atatgaaaat tggcttttct tgggggaaaa
     6541 gtttaagtaa tttagtagat ttaattcaca gtaaaagtgt ccgaaatgtt cacttctatc
     6601 ctctagcagg tggtcctagt cacatacacg ctaaatacca tgtgaataca ctgatttatg
     6661 aaatgtctag aaaatttcat ggagagtgta catttatgaa tgcaacgatt gtgcaagaaa
     6721 ataaattgtt agcagatggt attttgcaat caagatattt tgaaaatttg aaaaatagtt
     6781 ggaaagattt agatatagct gtagtcggaa ttggtgattt tagcaataaa ggaaagcatc
     6841 aatggttaga catgcttaca gaggatgact ttaaagaatt gaccaaagtt aaaactgtag
     6901 gagaaatttg ttgtcgattt tttgattcaa aaggtaaaga agtctatgaa aacttacagg
     6961 aaagaacgat agcaatctct ttggaagatt taaaaaatat tcctcaaagt ttagctgttg
     7021 cttacggtga tacgaaagta tcttcgattc tttctgtctt gcgtgctaat ttagtaaatc
     7081 atttgattac agacaaaaat acaattttaa aagttttgga agaagatggg gatttgactt
     7141 ttagagagat tctaggtgag tgaaaatgat agactgattc agtttatcgt ttttcttttt
     7201 agttgattgc acatttgtgc ttatataaac aaaaatagtt tatctgttgt ttttggattg
     7261 acaactttat tatgtagttg tattctatag ttacaaaaga aaattttaaa atttcaaatg
     7321 aaaaaagctt tttacatagt gaaatgagga ggaatttatg gaaattattg ttccagatca
     7381 aattatcatg ggtttaattt tatatgctgg cgatgcgaaa caacatattt ataaagcatt
     7441 agattacata aagaacggta catgtgaacg atgtgaagaa gaaatccagt tagctgatgc
     7501 agccttatta gaagctcata atctacaaac aaaattcttg gcacaggaag cgtctggtac
     7561 aaagacagaa attacagctc tctttgttca ttcacaagat catctcatga ctagtatgac
     7621 ggagattaat ttaatcaaag aaattattag tttgagaaaa gaacttcata aaaaataata
     7681 ctagagtatt atcattgtta ttaacataga ggaggaaaac ataatggtga agattggttt
     7741 gttttgtgca gcaggttttt ctactggtat gcttgtaaat aatatgaaaa ttgcagcgca
     7801 agctagtgga gttgaggcag aaatagaggc gttttctcag tctaaattag cggattatgc
     7861 gccaaatata gatgttgcac tattgggtcc acaagttgct tatacattag ataaatcaaa
     7921 agaaatttgt gataagtgtg atgttccgat agctgttatt ccgatgatgg actatggtat
     7981 gttagatggg aaaaaagtat tagatttggc cctatctttg attagtgggt aagaaaagga
     8041 gatttattat ggcaaagatg gatgttcaga aaatcattgc accgatgatg aagtttgtga
     8101 atatgcgtgg cattatagct ctaaaagatg ggatgttagc aattttgcca ttgacagtag
     8161 ttggtagttt attcttgatt atgggacaat tgccgttcga aggattaaat aagagcattg
     8221 ctagtgtttt tggagctaat tggacagagc cgtttatgca agtatattca ggaacttttg
     8281 ctattatggg tctaatttct tgtttttcaa ttgcctattc ttatgctaag aatagcggag
     8341 tagaggcttt accagctgga gttctatctg tatctgcatt ctttattttg ctaagatcat
     8401 cttatatccc taaacaaggt gaggcaattg gggatgctat tagtaaagtt tggtttggag
     8461 gtcaaggaat tatcggtgct atcattatag gtttggtagt aggaagtatt tataccttct
     8521 ttataaagag aaaaattgtt attaagatgc cagaacaagt tccacaagct attgccaaac
     8581 agtttgaagc aatgattcca gcatttgtaa ttttcttact ttctatgatt gtatatattt
     8641 tagcgaagtc attgactaat ggcggaacat tcatagaaat gatttattct gctattcaag
     8701 ttccattgca aggtttaact ggatctttgt atggtgcaat tggaattgca ttctttatat
     8761 catttttgtg gtggtttggt gttcatggac aatcggtagt aaatggagta gtgacagctc
     8821 tgcttttatc taatcttgat gctaataaag ctatgttagc ctctgctaat ctatcattag
     8881 aaaatggtgc acatattgtt actcaacaat ttttagattc atttttaatt ctatcaggtt
     8941 cagggattac gtttggtctt gtagttgcca tgctttttgc agcaaaatca aaacaatacc
     9001 aagccttagg aaaagttgca gcttttccag caatatttaa cgtaaatgag ccagttgtat
     9061 ttggatttcc gattgtcatg aatccagtta tgtttgtacc tttcattctt gttcctgtac
     9121 ttgcagctgt gatagtatat ggaactattg caacaggttt catgcagcca ttctcagggg
     9181 taacattgcc ttggagtaca ccagctattt tatcaggatt tttggtgggt ggatggcaag
     9241 gagttattac tcagctggtg atattagcga tgtctacatt ggtttatttt ccattcttta
     9301 aagtacagga tcgtttagct taccaaaatg aaatcaaaca atcttagagg tatttgtgtg
     9361 ttactgttaa actcacacat ttgtgctaaa aattagagag ttaaaatttt tctagttaaa
     9421 agcttgaaaa tttctataaa aatcggtatt atattttcga aagaaataaa aatattttcg
     9481 aaagaaaggt ggttacgatg gtaaatacag aagtagcaag aacaacaatc aagacagaat
     9541 attttggcag ccttactgaa aggatgaaca aatatcgaga agatgtttta aataaaaaac
     9601 cttatattga tgctgagaga gcagttctag caacacgcgc ctatgaacga tacaaggaac
     9661 aacctaatgt cctaaaacgt gcatatatgc tgaaagaaat tttggaaaat atgactatct
     9721 atattgaaga agaatctatg attgcgggaa atcaagcttc ttccaataaa gatgctccta
     9781 tttttccgga atatacgcta gaatttgttc tcaatgagtt ggatcttttt gaaaagcgtg
     9841 atggagatgt tttctatatt acagaagaaa caaaagaaca acttagaagt attgctccgt
     9901 tttgggaaaa taataattta cgtgctagag ctggtgcctt attacctgaa gaagtgtctg
     9961 tttatatgga aacaggattc ttcggtatgg aaggtaagat gaattctgga gatgctcacc
    10021 tagcagttaa ctatcagaaa cttttgcaat ttggcttaag aggttttgaa gagcgggctc
    10081 gtaaagcaaa agcagctcta gatttaacag atccagcaag tattgataaa tatcattttt
    10141 acgactctat atttatcgta atcgatgcta ttaaagtata tgcaaagcgc tttgttgctc
    10201 ttgctaaaag tttagccgaa aatgcaaatc ctaaacgtaa gaaagaatta cttgagattg
    10261 cagatatttg ctctagagtc ccatatgaac cggcaactac ttttgcagaa gctattcaat
    10321 cagtttggtt tattcaatgt attttacaaa ttgaatctaa tggccactct ctttcatatg
    10381 gccgttttga tcaatatatg tatccatata tgaaggctga tttagaaagt ggtaaagaaa
    10441 cagaagatag cattgttgaa cgtctgacaa atctttggat taagacaatt acaattaata
    10501 aggttcgcag tcaatcacat acattttctt cagcaggaag tcctttatat caaaatgtta
    10561 caattggtgg acagactcga gataagaagg atgctgttaa cccattatct tatttggtat
    10621 taaaatcagt tgcacaaacc catctaccgc aacctaatct aactgtacgt taccatgcag
    10681 gtttagatgc tcgtttcatg aatgagtgta ttgaagtgat gaaacttggt tttggtatgc
    10741 ctgcatttaa taatgatgag attattattc cttcttttat tgcaaaagga gtattggaag
    10801 atgatgctta tgattacagt gccattggat gtgttgaaac ggcagttcca gggaaatggg
    10861 gctatcgttg cacaggtatg agttatatga acttccctaa ggttctactt atcacgatga
    10921 atgatggaat tgatccggct tcgggtaaac ggtttgcacc aagctttggt cattttaagg
    10981 atatgaagaa cttttctgaa ttagaaaatg cttgggataa aacactaaga tatttgacac
    11041 gaatgagtgt tattgttgaa aattctattg atttatcatt ggaacgagaa gttcctgata
    11101 ttctatgttc agcattgact gatgattgta ttggtcgtgg aaaacacctt aaagaaggtg
    11161 gagcagtata tgattatata tcaggattgc aagttggaat tgcaaatttg tcggattcat
    11221 tagctgcaat taaaaaattg gtgtttgagg aagaacgtat aagcccaagt cagctttggc
    11281 atgcactgga aacagattat gccggagaag aaggtaaggt cattcaagaa atgttgattc
    11341 atgatgcacc taagtatggt aatgatgatg attatgctga caaattggtt actgctgctt
    11401 atgacattta tgttgatgaa attgctaaat atcctaatac acgttatgga agaggtccaa
    11461 ttggaggaat tcgttattca ggaacatctt ctatctcagc caacgtaggg cagggacgtg
    11521 gaacattagc aactccagat ggacgcaacg cgggtacacc gttagcagag ggttgttcac
    11581 catcacataa tatggatcaa cacggcccta catctgtttt aaaatctgtt tcaaaattac
    11641 caacagatga aatcgtaggt ggggttctct taaatcagaa agtaaatcct caaacgttag
    11701 ccaaagaaga agataaatta aaactaattg ctttgttacg aacattcttt aatcgtttac
    11761 atgggtacca tattcaatac aatgttgttt ccagagagac gctgattgac gctcagaaac
    11821 atcctgaaaa acacagagac ttaattgttc gtgttgcagg atactctgca ttcttcaatg
    11881 ttctttctaa ggcaacccaa gatgacatta taggacgtac tgagcatact ttgtaaaata
    11941 aagaggttct ttttatggaa tttatgcttg acacattaaa tttagatgag attaaaaagt
    12001 ggtctgaaat tttgccgcta gctggggtaa cttcaaatcc cactattgca aaaagagagg
    12061 gttctattaa tttttttgaa cgaatcaaag atgtaagaga attgattggc tctacaccct
    12121 ctattcatgt tcaggtgatt tctcaagatt ttgaaggcat cttaaaggat gctcataaaa
    12181 ttcgaagaca agcaggagat gatatattta tcaaagtacc tgttactcca gctggattac
    12241 gtgcaataaa ggcgctaaaa aaagagggct accatatcac tgcaacagct atttatacag
    12301 ttattcaggg attattagct atcgaagcag gagcggatta cctagctcca tattataata
    12361 gaatggaaaa tctgaacatt gattcaaatt ctgtcattcg tcaattagct cttgctattg
    12421 atagacagaa ctctcctagt aagattttag ctgcatcctt taaaaatgta gcacaagtaa
    12481 ataatgcttt agctgcaggt gcgcatgctg ttacagcagg agcggatgtt tttgaatcag
    12541 ctttcgccat gccatctatc caaaaggcgg ttgatgattt ttctgacgat tggtttgtta
    12601 ttcaaaatag tcgttccatt tagatagaga ggaaatacat atgagaattt ttgctagtcc
    12661 ttctagatat attcaggggg aaaatgcctt gtttgaaaat gccaaatcaa ttttggattt
    12721 gggaaattgc cctattctat tatgcgatca gttggtttat gatattgttg gaaaacgatt
    12781 tgaagattac ctacataggt atggtttcca tattgttctg gcgctattta atggtgaagc
    12841 ttctgacaat gaaatcaatc gagttgttgc cttggctgag aaagaaaatt gtgatagtat
    12901 tatcggtctt ggtgggggaa agacgattga tagcgcaaaa gctattgcag atttgattga
    12961 aaagcctgtt attattgctc caacaattgc atcgaccgac gcacctgtat ctgctttatc
    13021 tgttatttat acagatgaag gtgcatttga tcattatcta ttttattcta aaaatccaga
    13081 tttagttttg gttgatacaa aagttatttc acaagcccct aagcgtttat tagcgtctgg
    13141 tattgcagat ggtttagcaa cttgggttga ggcgcgtgcg gttatgcagg caaatggaaa
    13201 aactatgttg ggacaacagc aaacattggc tggagttgca attgcgaaga aatgtgaaga
    13261 aacgctgttt gcagatggtt tacaggctat ggcagcttgt gaagctaaag tggtgacacc
    13321 agcattagaa aatattgttg aagctaatac tttattgagt ggtctaggtt ttgaaagtgg
    13381 aggattagct gcggcgcatg caattcataa tggttttact gcattgacag gtgacattca
    13441 tcatttaaca catggtgaaa aagtagctta tggaacttta gtacaactat tattggaaaa
    13501 tagacctaaa gaagaacttg ataagtatat tgagttttac aaaaaaattg gtatgccaac
    13561 aactctaaaa gaaatgcatt tggatcaagt tggatatgat gatttaataa aagttggtaa
    13621 acaagcaact atggagggtg agacaattca tcagatgccg tttaagattt cgccttcaga
    13681 tgttgctcaa gctattatcg ctgtagatgc ctatgtaaat tcaaaataaa caataaggac
    13741 tactgttttc caaatggtag tcttttattg atccctgtat tgaattctat agaagattga
    13801 aataggatga gaacaaatcg attgggaaag taaaattaat ttctataaat gttttagcaa
    13861 ttgtttcgta ctatttcaga ttcagtctac tatacgttct tcataaatca aaaagcgaca
    13921 taggtagtcg gctatttatt gtgagtacat taattagcat tccagtttta tcttcggtct
    13981 aaaataagta ttttgtgcta tacgagataa gcttcttgac ttactccttg atttactgca
    14041 taacaatggg ataaaaagtg ggagatagag caattcatag tcatcaaaat taatgagata
    14101 cagtatacag tttttccttt aaacacattt caaattccct caaaaatggt ataatagtaa
    14161 catcacaaaa ttggagagag accatgagtt tttacaatca taaagaaatt gagcctaagt
    14221 ggcagggcta ctgggcagaa catcatacat ttaagacagg aacagatgca tcaaaaccta
    14281 agttttatgc gcttgatatg ttcccttatc cgtctggagc tggtctgcac gtaggacacc
    14341 cagaaggtta tactgcaacc gatatcctca gtcgttacaa acgtgcgcaa ggctacaatg
    14401 tccttcaccc aatgggttgg gatgcttttg gtttgcctgc agagcaatac gctatggata
    14461 ctggtaatga cccagcagaa tttacagcgg aaaacattgc caacttcaaa cgtcaaatca
    14521 atgcgcttgg attttcttat gactgggatc gtgaagtcaa tacaacagat ccaaactact
    14581 acaagtggac tcaatggatt ttcaccaagc tttacgaaaa aggcttggcc tatgaagctg
    14641 aagtgccagt aaactgggtt gaggaattgg gaactgccat tgccaatgaa gaagtgcttc
    14701 ctgacggaac ttctgagcgt ggaggctatc cagttgtccg caaaccaatg cgccaatgga
    14761 tgctcaaaat cacggcttac gcagagcgct tgctcaatga cttagatgaa ctagattggt
    14821 cagagtctat caaggatatg caacgcaact ggattggtaa atcaactggt gccaatgtaa
    14881 ctttcaaagt aaaaggaaca gacaaggaat ttacagtctt tactactcgt ccggacacac
    14941 ttttcggtgc gactttcact gtcttggctc ctgaacatga attagtagac gctatcacaa
    15001 gttcagagca agcagaagct gtagcagact ataaacacca agccagcctt aagtctgact
    15061 tggctcgtac agaccttgct aaggaaaaaa ctggtgtttg gacaggtgcc tatgccatca
    15121 accctgtcaa tggtaaggaa atgccaatct ggattgcaga ctatgtcctt gctagttatg
    15181 gaacaggtgc ggttatggct gtgcctgccc acgaccaacg tgactgggaa tttgccaaac
    15241 aatttgacct tccaatcgtc gaagtacttg aaggtggaga tgtcaaagaa gctgcctaca
    15301 cagaggatgg cctgcatgtc aattcagact tcctagatga actcaacaaa gaagacgcta
    15361 ttgccaagat tgtggcttgg ttggaagaaa aaggctgtgg tcaggagaag gttacctacc
    15421 gtctccgcga ctggctcttt agccgtcaac gttactgggg tgagccaatt ccaatcattc
    15481 attgggaaga tggaacttca acagctgttc ctgaaactga attgccgctt gtcttgcctg
    15541 taaccaagga tatccgtcct tcaggtactg gtgaaagtcc actagctaac ttgacagatt
    15601 ggcttgaagt gactcgtgaa gatggtgtca aaggtcgtcg tgaaaccaac actatgccac
    15661 aatgggctgg ttcaagctgg tactacctcc gctatattga tccacacaat actgagaaat
    15721 tggctgatga ggacctcctc aaacaatggt tgccagtaga tatctacgtg ggtggtgcgg
    15781 aacatgctgt acttcacttg ctttacgctc gtttctggca caaattcctc tatgacctcg
    15841 gtgttgttcc gactaaggaa ccattccaaa aactctttaa tcaagggatg attttgggaa
    15901 caagctaccg tgaccaccgt ggtgctcttg tggcaaccga caaggttgaa aaacgtgatg
    15961 gttccttctt tcatgtagaa acaggggaag agttggagca agcgccagcc aagatgtcta
    16021 aatcgctcaa gaacgttgtt aacccagacg atgtggtgga acaatacggt gccgataccc
    16081 ttcgtgttta tgaaatgttt atgggaccac tcgatgcttc gattgcttgg tcagaagaag
    16141 gtttggaagg aagccgtaag ttccttgacc gagtttaccg tttgattaca agtaaagaaa
    16201 tccttgcgga aaacaatggt gctcttgaca aggtttacaa cgaaactgtt aaagctgtca
    16261 cagagcaaat tgattccctc aaattcaaca cagccattgc ccaacttatg gtctttgtca
    16321 atgcggctaa caaggaagac aagctctatg ttgactatgc caaaggattt atccaattga
    16381 ttgccccatt tgcacctcac ttggcagaag aactctggca aactgttgct gcaacaggtg
    16441 agtcaatctc ttacgtgact tggccaacat gggacgaaag taaactggtt gaagacgaaa
    16501 tcgaaatcgt tgtccaaatc aaaggtaaag tccgtgccaa actcatggtc gctaaagacc
    16561 tatcacgtga agaattgcaa gaaattgctc tagcggatga aaaagtcaaa gcagaaattg
    16621 acggtaagga aatcgtgaaa gtgattagtg taccaaataa attggttaat atcgtcgtta
    16681 aataacgagt ttattagctc tatctgccac cttcaatagt ccactggact attgaagcca
    16741 actaaattag ttaacattgt tgtgaaataa aaagaagtaa ctctagagta tgaactttag
    16801 agttactttt agaataggtc agaatgaata gattagtgaa cgatagcagg aagtgtgaaa
    16861 ttttattatt taagatttct attattgtag tgtcagttat agtattagta atgtcaatag
    16921 tgggtgcata ttattttaaa aattgtcttt gggacaagga aagtttaaat ttgacactca
    16981 ctttagacac tctctttgca gtttttatta ctttctttag tttttcaaat gataaatcaa
    17041 gcaatatttt tggaatatct ccaaatgaaa ttataaggac acagtctgaa gtgtatcaat
    17101 ttttatttac aaaatctttt gcaactctat taatagtgac aaatttgtta tccattattc
    17161 attattttgg agccaaaaat ttaatagttt cttttaatga aacttatcct cgtattcaat
    17221 ccagtgttgg tatcatgtgg ctaccactgt ttggtattct gataatctat tttatttggc
    17281 tcttaataga gggattaaaa tatatcagaa aaataaatta tattattcaa aatagggaaa
    17341 taatctattt ttattcgcag gtaaaagttg tagataaact tactgattct gaatcgaaaa
    17401 aatacggtga atatattgat ttgtacgaat cgtgtcgtca gaacaattta agtgttctac
    17461 ttaactttct tcaaaattcg gatgataaac gaggagttat tgctagtaac tcagtgagaa
    17521 ttatgtttga atctttggat aaagcagacc aaattgcggc cctgaatatt tgtagaaact
    17581 atgtagcacg agaaaatgtt ttaaataaag aagatgcaga aaaattgtta aatgaaattg
    17641 ataaaaaact tgaaattagc agataggaca attcaaatag agttgagtaa aaagtttgaa
    17701 aagtgaaaca aggagaaagc tatgccagta aatgaatatg gtcagatgat tggtgagtca
    17761 atggaaggtt atacacccgg tgcactgcct tctattgatt tcttagaagg gcgttatgct
    17821 aggatagagg ctctctcggt agaaaaacat gcggaggatt tgctagctgt ttatggcccg
    17881 gatactcctc gggagatgtg gacctatctt tttcaggagc cagtagcaga tatgggggag
    17941 ctggttagcc ttttaaatca gatgttggct cgtaaggacc gtttttacta tgcaatcata
    18001 gacaaggcga ctggtaaggc tttgggaact ttttctctca tgcgcattga ccagaataac
    18061 cgagtaatag aagtcggagc agtcactttt tctccagagc taaggggtac acggatagga
    18121 actgaggctc agtatctcct agctcgctat atttttgagg agctaaacta tcgtcgctac
    18181 gagtggaaat gcgatgcctt aaatctgcca tccagacgag ttgcggagcg tttgggattt
    18241 gtctatgaag gaaccttccg ccaggcggtg gtttataagg ggcgtacgag agatacggat
    18301 tggttgtcta tgattgatat ggactggcct caagtcaaag atcgtttgga aacatggctg
    18361 cgtcctgaaa attttgataa aaatggacag cagtacaaga gcttgagaga actttaagag
    18421 gtgttgagat gattactatt aaaaagcaag aaattgtcaa gctagaggat gttttgcatc
    18481 tctatcaggc tgtcggttgg acaaattata cccatcaacc agagatgctg gagcatgcct
    18541 tatcgcattc attagtaatt tatctggcac ttgatggtga tgctgtggtg ggcttgattc
    18601 gtttggttgg agatggattc tcatcagttt ttgtacagga tttgattgtt ttaccaatct
    18661 atcagcgtca agggattggt agtgccctaa tgaaagaggc cttaaaagat tacaaagatg
    18721 cttatcaagt ccagctggtg acagaagaga cagaaaaaaa cgtgggattt tatcgttcta
    18781 tgggctttga aatcttatcc acctataatt gtatagggat gacttgggcg aatcgagaaa
    18841 aataacaaaa cttgtttttt cttaagcaaa atttaaggat ggtctagtat tatatagtca
    18901 ttaaataaag acctcctaac tttatttaat aaaatcctaa acttttttca tcacaatctc
    18961 ctaatgaagc cacccaatca ggtggctttt ttgcgtggtg aggtgctggt gatagaaatt
    19021 ttttgcaaaa tagtaaaatt ttagaaaagt taagctagtt ttaagtttcg tcttgtatca
    19081 tgtagt
//
LOCUS       DAVMMR010000038        18849 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMMR010000038 DAVMMR010000000
VERSION     DAVMMR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.038"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(358..1965)
                     /locus_tag="VYS74_001342"
     CDS             complement(358..1965)
                     /locus_tag="VYS74_001342"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV3789375.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2355..2942)
                     /locus_tag="VYS74_001343"
     CDS             complement(2355..2942)
                     /locus_tag="VYS74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV3789376.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3070..3519)
                     /locus_tag="VYS74_001344"
     CDS             complement(3070..3519)
                     /locus_tag="VYS74_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789377.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3524..3913)
                     /locus_tag="VYS74_001345"
     CDS             complement(3524..3913)
                     /locus_tag="VYS74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV3789378.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDRISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3940..4566)
                     /locus_tag="VYS74_001346"
     CDS             complement(3940..4566)
                     /locus_tag="VYS74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3789379.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4580..5143)
                     /locus_tag="VYS74_001347"
     CDS             complement(4580..5143)
                     /locus_tag="VYS74_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3789380.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5308..5542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5596..6099)
                     /locus_tag="VYS74_001348"
     CDS             complement(5596..6099)
                     /locus_tag="VYS74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV3789381.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6292..7122)
                     /locus_tag="VYS74_001349"
     CDS             complement(6292..7122)
                     /locus_tag="VYS74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3789382.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7107..8789)
                     /locus_tag="VYS74_001350"
     CDS             complement(7107..8789)
                     /locus_tag="VYS74_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789383.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8891..9103)
                     /locus_tag="VYS74_001351"
     CDS             complement(8891..9103)
                     /locus_tag="VYS74_001351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789384.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9110..9658)
                     /locus_tag="VYS74_001352"
     CDS             complement(9110..9658)
                     /locus_tag="VYS74_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV3789385.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9673..10521)
                     /locus_tag="VYS74_001353"
     CDS             complement(9673..10521)
                     /locus_tag="VYS74_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV3789386.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10710..12059
                     /gene="trkA"
                     /locus_tag="VYS74_001354"
     CDS             10710..12059
                     /gene="trkA"
                     /locus_tag="VYS74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV3789387.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIEREHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12063..13502
                     /locus_tag="VYS74_001355"
     CDS             12063..13502
                     /locus_tag="VYS74_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3789388.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13627..14085)
                     /locus_tag="VYS74_001356"
                     /pseudo
     CDS             complement(13627..14085)
                     /locus_tag="VYS74_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454376.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            14297..14467
                     /locus_tag="VYS74_001357"
     CDS             14297..14467
                     /locus_tag="VYS74_001357"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789389.1"
                     /translation="MTKRVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            15079..15363
                     /locus_tag="VYS74_001358"
     CDS             15079..15363
                     /locus_tag="VYS74_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789390.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLINVQKLMEKYKHG"
     gene            15356..15604
                     /locus_tag="VYS74_001359"
     CDS             15356..15604
                     /locus_tag="VYS74_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789391.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            15700..15918
                     /locus_tag="VYS74_001360"
     CDS             15700..15918
                     /locus_tag="VYS74_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789392.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(16286..18610)
                     /gene="pflB"
                     /locus_tag="VYS74_001361"
     CDS             complement(16286..18610)
                     /gene="pflB"
                     /locus_tag="VYS74_001361"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV3789393.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         5680 a         4069 c         3470 g         5630 t
ORIGIN      
        1 tggattaaat taaaaaagta taatgtattg aatctataac agtacatctt gactgctaaa
       61 atatttctat aaattaattt gacttttctg atagagatgt tcacatctta tttcaattca
      121 ctatacaatc cagttcctaa aactttgcta gaaattgatt tggctttccg aattaatttg
      181 ttcaaatttt atttcatttc aatatactga taaatccatt cactagcaca gggcaagagg
      241 gagtcgctat tgatttcaac ggtagcaggt acatacagag gacccctacc tcagatatac
      301 tgcaatatca tcgtatgcaa aaaacctctg agatttttct caaaggttct gattttgcta
      361 attgctgttc tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg gtcggtttgg
      421 acggcttgac agttcagggt gatactgaca agctacaaag aatttatttt caggaatttc
      481 tacgatttct accaaacgat tgtctggaga aactcctgaa aagacaaaac ctgctgcctc
      541 aaactgctca cggaaggcat tattaaactc ataacggtga cggtgacggc gttgcaccac
      601 ttcttgattg tgataagcag cagcagcctt agagccacgt ttcaacttag acggataaag
      661 tcccaaacga agggttccac ccatatcctc aatatcaatc tgatcacgca tgatatcaat
      721 gatagggtat tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa gacctaaaac
      781 gtgacgagca aactcgatac atgtcaactg cattcccaag cagactccca acattggaac
      841 atcattttca cgcgcatagc ggatggcttg gattttccct tctgtaccac gttgaccaaa
      901 accacctggt acgatgatcc cgtccgcatc agacaagagt tctgctacat tctctgctgt
      961 cacatcattg gcattgaccc aattgatttt aacttctgca tcattgacat agccagagtg
     1021 tttcaaggct tcgaccactg agatataggc atcttgcaac tccacatact taccaacaag
     1081 ggaaatctta acttgtttct tgaggttcat gaccttatcc accatggctg accattctgt
     1141 catagccgct gctggtgcgt ctaatttcaa atgatcacaa acaatttggt ccatcccttg
     1201 tgcctgcaag ttcagtggaa tttggtaaag gtgttcaaca tccaacgatt cgataacggc
     1261 ttctggtgcc acatcacaga actgggccag tttattttta attccttgac cagctggctc
     1321 ttctgtacga ataaccaaca tatttggttg gattcccaag ccacgcaatt ctttgacaga
     1381 gtgttgggtt ggtttggttt tcatttcacc agcagccttg aggtaaggaa gcaaggttgt
     1441 atggatatac atgacattat ccgcacccac atctgccttc atctgacgaa gagcctctag
     1501 gaatggcaag gactcgatat ctcctactgt tccaccaacc tctgtgataa tgacatcaga
     1561 gtcggtcgtt agagcggcac gcttgatttt ttctttcaaa gcatctgtga tatgaggaat
     1621 gacttgaaca gttgccccaa ggtattctcc acggcgttct ttacgaagaa cttcactata
     1681 aattttccca gttgtcacgt tggaatattt gttgagattg atatcgatga aacgttcata
     1741 gtgacccaag tccaaatctg tctcagctcc gtcatctgtc acaaaaactt ccccgtgctg
     1801 gtaaggactc atggttcccg gatcaatatt gatataaggg tcaaactttt gaatggttac
     1861 tttgagacca cgatttttca agagacggcc tagactcgct gccacaatcc ctttcccaat
     1921 agacgatacc acaccaccag ttacaaaaat atatttcgta gacatagatt cctctttcta
     1981 aaatgctcaa ggtcttgtta actacgaggg gatatagaaa acaagaccct actaataaga
     2041 ataatctggg acaactgcac cagattcaca actaatactc ttcgaaaatc tcttcaaacc
     2101 gcgtcaacgt cgccttgccg tagatatgtg ttcctgactt tgtcagtctt atctacaacc
     2161 tcaaaacagt gttttgagca gcctacggct agtttcctag cttgctcttt gattttcatt
     2221 gagtataaaa tacaagaaga taacttcttg aaaaaacaaa aatagctccc taagaactag
     2281 ggagccccga cctctaaaag aggtgcccaa acaatatgat acctaaaaat aggataattg
     2341 tcaatagcta actcttattc ctcgctggtt tcagcgtcgt catctgaaaa ctcgtcgtcg
     2401 tcttcgttga gatccacatc ttctcccaag tcatctggag cgatttcgtt gatttctgca
     2461 tcataagctt ccacttcatt tttttcatca tctggatttt catcatcgta tgaaagagct
     2521 ggatctgctt cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc aatggcatct
     2581 gaatcaccat ccataaaggc attgacacgt tttttcttag cttttggtgc tacttcatcg
     2641 tcgtcatttt cttcaagagc gatgatttct tcgtcgattt cgtccacacc ataccatgaa
     2701 cgaagacccc atttgttgtc cccaagtgag atgaagctac cgtcaaagtt caactctgtg
     2761 tagaacaaag gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta gttttgaatt
     2821 tcgtttacaa gatcgctaaa atgcatctca tgatcgcgac cacgaagttc caagatagca
     2881 cgcgctacct caatcataga tagttcactt ttttcttgcc cagcaaatac ttctaattcc
     2941 aaagcgtttc tcctcattta tactactatc gccagagcga acagactctg acctcatttt
     3001 atcatttact ctttatttta cgataatttt gcggaatagt caaaggttaa gggggagaaa
     3061 gtggcaggat tagactaatt ccaatataaa actccttcct ttttctgttg ctccattttc
     3121 cacaaatcca agcgacttga aacacctcct agaagcatga ttgtaggtgt agatttcctt
     3181 gactctcaat tctttccatc cttttactcg agccaattca atcaaagcac ttagaatctt
     3241 ttttccaagt cctcgatgtt ggtaagcgga attcccaatc acaatgggga gattatcctg
     3301 agatagtgta atatccccaa ttggaaacca ttctcccttc tccttgactt caatccaaaa
     3361 aagctctcca tgccgatcca aataggaata catggcttcc aaggtcgctt gactgtaagg
     3421 aagcttcacc ccatctacga ggtaaaccaa gttcacatcc tgataccaag caaaagcttc
     3481 ctcacaatga ttgaccttat aataaggaac aaaacacaat gcactacctt ttcaacaaaa
     3541 gagttgctgc ttgattaaaa ccatcacacc agttatacca ttttgcttca tacccatctt
     3601 gagctaggat acgatctttt aaatcaaaaa cagagtaaat ctttctttcc tcgcaagctt
     3661 gcgcatagag atgatatagt tcatcaccac catctctatc ccactcagca gaaatcgtat
     3721 cccgacctgc caataaagcc tgataagccc tgtgatgccc atctgtaatc agcaaacaat
     3781 ctccaaaggc aagaatactg attcgatcga cttggattgt ttctgccgac tggtaaagca
     3841 tctgaatatc ttgcaacttc ttttctgata aatatagttg agtcggatga agatctgcta
     3901 tattgacttt catttctttc tctcaaggga attcgatact cacttctgtt tgcctttaaa
     3961 tcgccattgg aagcggagct tgtcataaaa gggaaactcg ataaacagga ctcccaagcc
     4021 cacacagaga ctggcaagga cgtctgatgg gtaatgaact cccagataga ctcttgatac
     4081 cagcacactg actaggtaga ggccaaggac gatttgtacg atttttctcc agacctgatc
     4141 tttaatccgc tgactaagaa taacaatcaa agtccctacc atcagcgtta cagctagaga
     4201 atgcccactt gggaaggaaa atcccttctc ctccaccaga tgtaaaatag ctggtcgtgg
     4261 gcgctggtag atatttttaa aggtcacgat taaaagacct gccaaagcca gatttcccag
     4321 catgaagaaa ctttctatct tccatcgctt acgataaaag acaaaagctg taatgacaac
     4381 ccaagtgata atcactggga tatcaatcag acgtgtgagg gctcgaaaaa gaatagtcaa
     4441 ataatctggt aagtctcctc gaatggcagt ctgaatcgat tggtcaaaat tgaccaacat
     4501 ttcagggtaa aatttgacca tatagccaag aataacgaaa agtaaaaggg caaaactgcc
     4561 cttcattaaa aatgtttgtt tatctttcat agtgttttaa ggttggtttc aaaaggacgt
     4621 acaacaacca gaatgaaacg gaaaagatta caccctcaat caagttaaaa ggtaatacca
     4681 tggtcattag gtagttggaa agtcccaaaa tttttccaat atcaaagtta gcaaacttag
     4741 cgtacaaagg aacagcataa acatagttga gaaccaacat ggccaaggtt aaaccaatag
     4801 ttccagctag agagcctagt aggaaacgaa gggttgtccg ttcctttttc caaatcaaag
     4861 caaatacgat gacaaaaact cccaaagcta cgatattcat cggcaaacca atgtaagtat
     4921 tcactccttg gctgttaaga agcaatttca agagtgagcg aagcaagagc actcctagag
     4981 cagcaggcaa atccatgacc accagaccca caaggactgg caagatacta aattcgatct
     5041 tgaggaaaga tgccgctggt aaaagcggaa agtcaaagta catcagcaca aatgagatgg
     5101 ctgatagaat tgcaatggtc gaaagtcgac gtgtgtttgt cataacaggt tcctccaatt
     5161 ttctataaaa tcagaagaag ttggaaagga ttcctctatc tattctcact ttttatatcc
     5221 caaaagttcc ctcttactct attaaagaaa aacaaagcaa gtggttacaa tccggctata
     5281 aatctatcaa aacagacaaa gctattcttt cgtcttctcc catccagact atactgtcgg
     5341 ttgtggaatc tcaccacatc agcttgcgct cgcggacttc tttaaagaga aagaaataga
     5401 gatttttctt ttagactagg cgacaagctg taggcataac ttctggttag gcaaagcttg
     5461 tcaacaaagt ataaaaggaa aagatcattt cctagttaaa gtcaccgccg gtcgggaatc
     5521 acaccctgcc ctgaagactc ttttatcata acaaaaaacg cttgcaagcg caagcatttt
     5581 gttcattttc attttttatt tcaatttatc cacttcatag gtatggacga gctcaagacc
     5641 tgcaaagttc tgctggcgaa gagcctcata gacaatcatg cagacggtat tagacacatt
     5701 gagactgcgg acatgttcat cattcatggg aatacggaga gccttctcag gatgttctcg
     5761 cataaaatct tcaggtaagc ccttgtcttc acgtccaaag agaaaataat ggtcctcgtc
     5821 agtcgataaa tccacctcag aatagatttt ttccgcgaat ttcgaaatca gatagagttt
     5881 tcccttcatc tgagacatga aatcttctaa actctcgtaa aaataaatct ctagcttatc
     5941 ccagtaatcc aatccagccc gcttcatctt gcggtcatca ataggaaagc ccataggctt
     6001 gatgatgtgg aggggagaat tggtcgcagc gcaagtacgc gcgatattgc ctgtattttg
     6061 tggaatttga ggttcaaata atacaatgtg atttgtcatg acttgcttcc tttcaccatt
     6121 gcaaaaaaat agccacactg cccggagtca agctcagcaa acagcgtggt taaggcatcg
     6181 ttaacttacc tcacaacagg tttgaagtaa atcagcgaaa ctactttctt agtataacac
     6241 tttcagaatc attgtcaata gaaatgactt gattttttca attttttcaa gctatttcca
     6301 agggttgtaa aatcgtccct gattctgcaa gataagtagt aaactaacta ctaaaaacaa
     6361 ggttgccaag agcaaggtaa tatagtctcc ttttttcaag gcctgataac tataccatgt
     6421 gcgttttttc tctttcccaa agcggcgaag ctccatggca gtcgcaatgg tatcaatgcg
     6481 ttctagcgag ctaaaaatca agggcgtaat aatgagcaga ttgcctttga ttcgttgcat
     6541 aagagaagct ttcttggata attccatccc acgcgcctcc tgagacatct tgatagtaaa
     6601 gaattcttcc tgcaaatctg gaatatagcg caaggtcagg ctgacagaat aagcaatctt
     6661 atagggcaca ccaatttgat ttaaactgga agcaaactga ctaggatggg ttgtcatcaa
     6721 aaagataata gccagaggaa tggtgcaaag atacttaatg gccaaattta gcagataaaa
     6781 gagctcctgg ctggttagag tgtagacacc gattccctgc caaatcacac ttctctctcc
     6841 ataaagtcca accccatact cgggagaaaa gagatagacc atcaaaacgt ttaaaacggc
     6901 aaataccgtc gcaaaaacgg ctacaaagga aacatcttta aagcgaattt ctgataaata
     6961 gaggagaaag actgaaaaga tggcaatcag caagagcatt ctggtatcat agctaatcat
     7021 ggccgccaat gataccagaa taaaaaagag aagtttccca gctcctgaca agcgatgaat
     7081 cacagtatct ctatgctggt aaccgattaa tttagcttgc atccctctct cctttctttg
     7141 taaaatgccg ttaaatccag tggatccaca tctagtttct tagccaagtt aaagatggag
     7201 gtttctttta gattggcttt tactaacagc tcaggatcgc tcaacagact ggctggaaca
     7261 gtatcggcaa tcaattctcc atccaccatg acaaggaccc ggtctgaata atccagcatc
     7321 aattgcatat catgggtaat catgacaatg gtatgccctt tttgatgtaa ctcttcgaga
     7381 aattccataa tctcagtata gttcttctga tcttgacctg cagtcggttc atctaggaga
     7441 ataatttcag ctcctaagac caaaattgaa gcaatggtga cacgtttttt ctgaccaaat
     7501 gacagggcag aaataggcca attacggaat tcataaagtc cacagatttt caaggtttca
     7561 tagactctcg tttcaatttc cttctcatcc acacctcgca aacggagccc tagagccacc
     7621 tcatcaaaaa tcatattggt tgaaatcatt tgattaggat tttgtagcac atagcctact
     7681 cgttccgccc gctctgcaac agaatcgcct tttatatcct gtttttccca aagatagcgt
     7741 ccttccgtct gaataaagct acttatagcc ttggctagag ttgatttccc tgctccattt
     7801 tttccgacaa tagcaatctt ttcacccttt ttaatatcta aatgtaggga ttttaaaatc
     7861 ggtctatcat cataagagaa agacacgtcc tctaatttaa agagtgactg caattctgga
     7921 gtttcttttg ccagttcatt ctgcagctga acctgacctt ttgagataga catgttatcc
     7981 agattcgcta actgttcttc cttgactaag tccacaccta attgacggag agtcgttaga
     8041 taaaggggtt ctcgaattcc attttgagtc aataaatcag tcgcaagcaa ctggtcaggg
     8101 ctcccattaa aaaggatacg accatcgttt atcaagacaa tccgatccac agggcgatgc
     8161 agaacatcct ccaaacggtg ctcgataatc agagtcgtcg tcccctcttc cttatgaatc
     8221 tggtcaatca attcgataat atcctgacct gacttgggat ctagattggc gagtggctca
     8281 tcaaacaaga gaatcggact ttcatcaatc aagacaccag ccagactgac tcgctgcttt
     8341 tgtccacctg acaaatcctg aggacgctga tccagtaaag gaagaaggtc cagcttttca
     8401 gcccatttat aaacacgacc tttcatctca tctagggctg tcacatcatt ttccagagca
     8461 aacgccaaat cttctgccac agacaagcca ataaactgcc catctgtatc ctgcaaaact
     8521 gtgctaacca gatgagactt atcatagatg ctcatatcaa aggctacttg accctttatc
     8581 aaaaattctc catatgtctg acccttgtaa atattgggaa taatcccatt caaacactga
     8641 cccaaggtag atttacctga cccagatggt ccaacaatta agactttctc tcccttgtaa
     8701 atggtcaagt ctatcccttg caaggtcggt tcttgttgtg tttcataccg gaaagagaaa
     8761 tccttccact caattatagc ttctttcatc ttactctctt cattcgcttc ttagacttct
     8821 attttatcat aaatcaagcc cttcttgcag tctcttctct taaaatctta gcgccaaaaa
     8881 gattcctatc ctagcttact tgcctaacta atctataaac atcgaaaaag actagttgcc
     8941 cagccttccc catcatttta tactcttcga aaatctcttc aaaccacgtc agcttcgcct
     9001 tgccgtaggt atggttactg acttcgtcag tttcatctac aacctcaaaa cactgttttg
     9061 agcaacctgc ggctaacttc ctagtttgct ctttgatttt cattgagtat tagtcctttt
     9121 tcaaacttcc tgcacgagtt tgggttcctg cataggcaag taagagaaga gttcctgcaa
     9181 tagctacaga tacaccattg gcaattcccg caacaatccc ttgtgcaaat actttttctg
     9241 ctgcttcttg ataaatcaca acatctccaa gtggtgccaa gacaccccaa acaagggcat
     9301 ttgcaagtag ttgaatgaga ttaaaaataa gaatatcttt ccagtcaaaa acactattga
     9361 tcacgcgaac gtactttcta aaaagtccca caactaaacc aaagagtccg ctagcgataa
     9421 tccaagtcca ccatagacca taaccagcaa gagagtcctt gattgcatga ccaattaacc
     9481 cgacaagcaa accgataatc ggtccaaaaa taatagaaag tagcgcttgt accgcatact
     9541 gaagctggat gcttgtattt ggaacagggg ttggaatgtt gatcatcccg atgacgacaa
     9601 agagggcagc gccaattccg acagcaacaa cttgtttaat tgtaaatttg atttccatac
     9661 tattctccta ttttatcctt ctattttctt tatttcaatg gtccaagatg aaccgacacc
     9721 tacattatag gccttggcaa aggaaccttg gttgatagcc aaacctaaac gatagagaga
     9781 gttgatgtaa agaatgggtt gcccaattct cacatctgca aatgatttgc catagacaac
     9841 ctgattttga tagaccagca tatcagcatg atagatggtc acttcaaaac gatcaccaaa
     9901 ttctggttcc aacttgtaaa attcttcccg tgtgatagag gtccaaagcg aaccgaaacg
     9961 cacatccaga atatcaatgg ctcccttcac cagatgatct tctatgatgg tcgctacgac
    10021 tggaagctct acaatctgtt ccacactgag ctctggccct acttcctcaa aagtaatgtg
    10081 accactggcc agtttagcac cagtataggc atagacatca cgaccgtgga aggtataaga
    10141 atgctctgtg ttttgacgcc tattggccac ctcagaaatc tcacgaatgg ctacaatgcc
    10201 aacgtgtttc ttgataaagg aaagcgtccc attatctggc gtgacaatgt attgattttt
    10261 tgcagtcttg gcaactacac tcttacgttt cgaaccgaca cctggatcga caaccgatac
    10321 aaacgtcgtt ccctcaggcc agtaatccac cgtctgaaag agacgatagc tcccctcaaa
    10381 aatattataa ggcgtgatat cgtgcgtcaa gtgatggatt tttaaggttg gagactcttc
    10441 taaagccact ccaatcatag ccgataccgc accatcaacc agaccaaagt ctgattgtaa
    10501 taccagtaaa ttattattca ttttatctcc ttgtatatcc tttatcatac catatttcag
    10561 agaaagtcgc taatagctat ttcaattggt tagggtatag ttttaactta tagcaaattt
    10621 cctactaaaa actctggtta ttagctagta ggtgcatttt ttcttgaaaa agggtatgat
    10681 agttacatca tgaaaaagta ggttttatta tgaaaattat ccttgtcgga gggggaaaag
    10741 ttggttttgc cctctgtcgc tccttggttg cagaaaagca tgatgttttg ctgattgagc
    10801 aagacgaagc tgttctcaat catattgtca gtcgctttga tatcattggt atccttggta
    10861 acggggccga ttttgccatt cttgagcaag ccagcgtcca agattgtgat atctttatcg
    10921 ccctgactga gcacgatgaa gtcaacatga ttgcagcagt tctagccaag aaaatgggag
    10981 ctaaagaaac tatcgttcgg gtgcggaacc ctgaatattc taactcttat ttcaaggaaa
    11041 agaatattct cggtttttct cttatcgtta atcctgagct cttggctgcc cgcgctatcg
    11101 cgaatatcat tgacttcccc aacgccctgt ctgtcgaacg ctttgctggt ggacgcgtta
    11161 gcctcatgga atttgtcgtc aagtccacca gcggtctttg ccaaatgccc atttctgatt
    11221 ttcgtaaaaa atttggtaat gtcattgtct gtgcgataga gagggagcat caaattatca
    11281 ttccaagcgg tgacatgact gtacaggata aagatagaat ctttgtcact ggtaaccgtg
    11341 tcgatatgat actcttccat aattatttta aatcacgcgc cgtgaagagc cttctcatcg
    11401 ttggggcagg tagaattgcc tattatctac ttggtattct caaagatagt cgtatcgata
    11461 caaaagtcat tgaaatcaat cctgaaatcg ccagcttctt tagcgagaaa ttcccaaatc
    11521 tctacatcgt tcaaggagat ggaaccgcaa aagatatcct gctggaagaa agtgctcaac
    11581 actatgatgc cgttgcgact ctaacaggtg tcgatgagga aaatctgatt acatctatgt
    11641 tccttgacag ggtaggtgta cagaaaaata ttactaaggt caatcgtacc agtctcctcg
    11701 agattatcaa tgcgcctgat ttttcaagta tcatcacacc taaaagcatc gctgtagata
    11761 cgattatgca ctttattcgt ggtcgagtta atgcccagta ttcagacctt caagccatgc
    11821 accatctagc caatggccaa atcgaaaccc tgcaattcca tatcaaggaa gccaataaaa
    11881 tgactgccaa acctctttct caactgaaat tgaaaaaagg ggttcttatt gcagccatta
    11941 ttcgaaaggg caagactatt ttcccaactg gggaggatat gttggaagtt ggagacaagc
    12001 tcctagtaac aaccttgttg ccaaacatca ccaagattta tgacttgatt gcgaggtaag
    12061 aaatgaataa aagtatgatt cgttacctcc tttcaaagtt acttttgatt gaagctgttc
    12121 tcctcttggt tcctgtggct atcgctgtct attaccgtga atcgagccaa gtctttacag
    12181 ccctcttttc gaccataggg attctcgtat tgttaggcgg ttcagggagt ttacagaagc
    12241 caaaaaatca acggatttat gccaaggagg gagtcttgat cgttgccctc tgttggatcc
    12301 tttggtcttt ctttggtggt ctcccctttg tcttttctgg gcaaatcccc agccttattg
    12361 atgccttttt tgaaattagt tctgggttta caactactgg agcaagtatt ttgaacgacg
    12421 tttcggttct cagccgttcc ctcctcttct ggcgaagttt tacccacttg attggaggga
    12481 tgggagtgct tgtttttgca cttgctatta tggacaatgc caaaaatagc cacctagagg
    12541 tgatgaaggc tgaggttcca ggtcctgttt ttggcaaggt tgtatccaaa ctaaaaaaca
    12601 ctgcccagat tctctatctc ctttatctag ctctcttctc cctctttgtc atcatctatt
    12661 atctagctgg tatgcctctc tacgatagtt ttgtcattgc tatggggaca gcgggaactg
    12721 gaggctttac cgtctataac gacggaattg cccactatgg cagctcactg attacctatc
    12781 tggtcagtat cggagttctg gtttttgggg taaatttcaa cctctactac tacctcatgc
    12841 tccgtcgcat caaggccttc tttggtgacg aagaacttcg ggcttacttg gtcattgtac
    12901 tggtttctac aggcttgatt agcctcaaca ccctctacct ctacccaggg ttttcaaaga
    12961 gctttgaaat gaccttcttc caggtttcca acatcattac aacaactggt tttggatatg
    13021 gagatattac caactggccc ctcttctctc agtttatcct ccttttcctc atggcaatcg
    13081 gtggctctgc tggatcaact gcaggtggac tcaagattat tcgaggcctc atcctttcaa
    13141 aaattgccaa aaaccaaatt ttgtcaatcc tatcgcccca ccgtgttttg accctccatg
    13201 tgaatcaaac ggtgattgat aaagataccc agcataagat tctcaagtac tttgtcatct
    13261 atgctatgat tttgctagcg cttatcttta ttgtcagcct agatagcaat gattttctgg
    13321 tcgtgaccag cgctgtcttt agctgtttca ataatatcgg gcctattcta ggaaccactt
    13381 ctagtttctc aatctttagt cctatctcaa aaattctcct ctcctttgca atgattgcag
    13441 gccgcttgga gatttaccca atcctacttc tctttatgaa gagaacttgg tctaagagat
    13501 aaaatacaaa atccctcttt ttacaattgt gaaagaggga tttttatatt aaaaaggggg
    13561 aacgttttta aatccaagcc tgtctgtaag caaagttctt actcaaataa aagatggaaa
    13621 aacttactac acagcttggt aatgctccaa tacaaattgt aaggctttac ttggattctt
    13681 tgttaaatga atatattcca ttcctctggt agcaactatc taaataccta atctttctgc
    13741 atccaccttc atctctctat aataactccg aacttgtgga gccagaataa ttaaatcata
    13801 aacacccata atatcataat gagctccgta cgctcctgaa ttcgcaatca ctctaacttc
    13861 tgctaattga gcgccctcgt taattgcatt ggctaattac gcacttgttc cagaccctgc
    13921 acaaagaacc agtactttca actccttact ttttattaca tctatctcac caggcattat
    13981 ttcacttgta tcctcctcta aaataatatc atttgagctt gcaatatcta caacctgtag
    14041 taaatatctc gtatttagac aatatgaaaa caagacgact tccatatagg aaaccgcctt
    14101 ctcgctatgt tgcatgaaac tgtaaaagtg gatgaaattg atagcggtag tcagatcaag
    14161 agacatcata actctaaaaa gtcacaatat ataagttcat cctcggaaaa atatcattct
    14221 aattgttgaa atggatatat aaacaggggt attaacatta aatgctcatg aaacaacgaa
    14281 tacaggtatc aaaactatga caaaacgagt ccctaaattt actaaagaca ctgctcaact
    14341 ttacacctgt aaatggttgt tgtataataa agttacaaag atgtacgacc acactgttgt
    14401 aaatcatagt gttcgcgaat atattactga tagcatttct acaaatacaa ttgaagagac
    14461 tggataggct ttaaacgaaa tatgtcagtg ttttggcatt cctagtcttc atatcattta
    14521 aagaattgta tagacaaaat ttttttccaa tacagacact cgtaacaact gcttcatttt
    14581 tctaccaaca tatttaggaa caggataaga tacaagaaga ttaatccata gctcagttct
    14641 ataccaatct aagacaaata agctaaaaaa cgattgataa taagcaaata gattccaaat
    14701 tttctctatc tgctcatttt aataaacaat actagtgtaa ctatccttcc agtcagaaac
    14761 ttgtcaaatc acaccgaaaa ctcttctaaa atttatctcg ttaggcaatc aagcaaaaac
    14821 tcgacgatag tacaaacatt atcatacaga attgacttcc taaattatat actttagtaa
    14881 ggttttcgga taagaaaaaa ggttcatttt acatttctaa acattctttt ctaagatgaa
    14941 aaacagaatt tttcgattgt gatttaaagc aacaagaaga ttttcagtgt catcctatag
    15001 atacgagcta attaagaaaa actacatttt tgaatataaa ctacaataat ataaactaaa
    15061 ttttatagga ggaagacaat ggattggtac gattatatga tacaggcatc caaacaatca
    15121 caattcaacg caagccattg gtttcgctat ttgcgaaaag ttatttttga agactattct
    15181 tatttaacaa accaagatgt agaaaagttg ctagactcca aagaactaac ccgttttcaa
    15241 aaaattagct tgaagtatgc ctttcaagag catactccaa ctcataaata tgtgatttca
    15301 ttaaataaac ctgctaagtt aatcaatgtt caaaaattga tggagaaata caaacatgga
    15361 taaaatgaaa ccggtcttcc aagccctaaa taaggaatta attcaggaaa atctgacttt
    15421 aacaattatc tgtgtcgatg gttatgtctt agaatatcat ggtttacgtg ccacacaaga
    15481 tgttgatgct ttttatgacc aaaatcagaa aatcaatgaa attattgctc gtgtaggaaa
    15541 acagtttaat cttaacatcc acgaagagtt atggctcaac aatcatgttg caaagcaaat
    15601 atgaacaaac aacctccctt aagtttatgc gaatccttgt actcatttga gaatctgacc
    15661 gttttagtag tccctattga gtatgtgtta ggaatgaaga tgatgagtat cagagagcag
    15721 gatttgcaag atattggtgc aattattaaa tacaaaaact ttcattcacc attcgatacc
    15781 tttaaatatt taaaagacat gggatttgat actattgacc tctctgttct attagaaggt
    15841 tttagttatg cttatggtat ggattggtta gaaaaatttt ttaaagaaaa tcaagataaa
    15901 cttcgcgaat tttattaaca gtatcaaaca agatgattgt caattagcca gtcgtcttgt
    15961 tttttatgat gaaatgaaat aaaatctgaa caaatcgctt agagaattcc agtcaatttc
    16021 tagcgctgtt tgagacgttt cagtggacta tagtaaaatg aaataagaat aggacaaatc
    16081 gatcaggaca gtcaaatcga tttctaagaa tgttttagaa ataaaggtgt actattctag
    16141 tttcaatcta ttatatttca gattcggtcc actattcctt cccaaaaaca ttgcatattg
    16201 cgtcaaagga attgctcaca ctatcccaag cacaaaaccc tcagtcgatt gaccgagggt
    16261 tcctttttat tattcaagaa cttgattagc tcaatgcatc caaggcgtca tccattgaaa
    16321 gaacttcgtg gaagacacgt tgtgtcaatt cagttttttg ttctggagtg aggtatttag
    16381 tgtttacaca gtatccagag atacgtacga taacgtcttc gcctgacatg atcttttcgt
    16441 aaacatcgtt caagtccata acgttcaagt taacgtgttg tccaccgttt tcgaagtaac
    16501 catcaaggat tgttaccaag ttatcaactt gttcatcacg agtcttacca agagcgcgag
    16561 gtgatacttg tgtagtcaat gagataccgt cagctgcata actaaagtca aggctagaaa
    16621 gtgagttcaa gttttgcaac caaccacctt tagctttgtt agatgggtta gcacctggtg
    16681 agaagaattc aagtttagac aagttcacag aaccatcttc gttgaggtat acacctttgt
    16741 gaactggtga gttaccagtt tgtttagagt aagcaacgtt agatgtgatt gtcaaaagtg
    16801 atactgtagc ttctgcgtct ttgtatagtt tgtggctacg tagacgagtt gtgtaagctt
    16861 cgatcaacca ttctgccaat tcgtttgaac gtgggtcatc ttcaccccag cgtgggtagt
    16921 caccgattgt ttcgtaatcg tagatgtagc catcttcgtc acggattggt ttaactgtag
    16981 cgtatttgat agctgacaat gtatcaacag tgttagcaaa tccacagata ccgaatccca
    17041 tgttggcacg ttgtttagtt ggcaagaagg ccatttgaac agcttcgtag ttgtacctat
    17101 cagtcatgta gtggatgatg ttcaaggcat ctacgtaagt gtcagtcaac cagtcaagag
    17161 atttttcaaa gttcgcttta actgattcga attcaagaac ttcgtcacgg attggttcga
    17221 tatcaaatac tttgtagtct ttgtgaacat cgtcgtaacc accattcaaa ccagtaagaa
    17281 gggctttaag aacgtttaca cgagcaccga agtactggat gttgtggcgt tgttcttcat
    17341 tttctggatc aagtggagac acacagcatg agatacagct catttcacca tatccgtctt
    17401 tagccattgt tgttacacct tcgtattgga tagaagagtg tttgtggctc atgtgcatac
    17461 agtagcgacg gaagttgtat ggcaatttgt cagtccaaag aactgtcaag tttggttctg
    17521 gtgagttacc gatgttgtca agagtgttca agaaacggta gtccatctta gtaacacggt
    17581 gacgaccgtc gttacccata ccagccatag aagttgtgat aaaggttggg tcacctgagt
    17641 acaattggtc ataagctttt gtacgagcaa atttaactgt acgaagtttc ataacgaaat
    17701 catcaacgaa ttcttggatt tctgattcag taaatgtacc acgagcaagg tcacgttctg
    17761 caaagatgtc caatacgatt ggtacacgac ctagagatgt agcagcaccg ttaatcacac
    17821 ggcagacagc catgaaagca atgttaaccc attggattgc ttctttcacg ttcatcgctg
    17881 gtttgcgaac atcaaccccg taaaggtcac ccaggcgaac aacttgttgc aatgcttggt
    17941 attgaaggtt tacttcttca cgaagacgga ttgtttcttc atcgatttct ttgattgcat
    18001 tccagtcgtt tactttttct tgcatcaagt agtctgcacc gtaaagagca agacgtgcgt
    18061 aaacaccgat gatacgtccg cgtgagtatg catctggaag accagttaca gtgtgtgcat
    18121 gacgagcgcg acgaatattt gaagtgtagg cacggaaaat accgtcgtta actgttgtta
    18181 catatttagt gaagatttcg tgaacagctg ggtctggttc gtatccattt tcttttaaag
    18241 tagtttcagc catacggata ccaccttttg gcatgaagtt caatttgaag agttcatcat
    18301 tttggatacc aaagataact tcattttctt tgtcgataaa tccagcaggg atatcagcga
    18361 tagatgttgg acgagtgtcc attgggaaac gagtttcttc gtagtgtgct ttagtttctt
    18421 ctacaatttt cttgatgtga agtgaacgct ctgttggtcc tgcaaggaag ctttcgtctc
    18481 catcataagg tgtgtagtta gcttgtacaa agcgtgatac acttgctttt tctttccaat
    18541 ctacgccttt gaagccttcc caagctttgt caaaaatatc ttgtgcttca acaactgtct
    18601 taacaaccat attaatatcc tcttttttct agtaacagtc atctgttaca ttcataagac
    18661 aagtatacca taaagtaacc gatttcaaca agtgcaaaat ccctattttt acactttctt
    18721 ttctaaaaca gtctatattt tatttcaaac tgtattatat ttttgaaaaa aataaagtcc
    18781 ttttttcttt ttttcagaaa aaagggtata ataaaagaaa ataagcagta acactcaatg
    18841 gaaatcgaa
//
LOCUS       DAVMMR010000039        18481 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMMR010000039 DAVMMR010000000
VERSION     DAVMMR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.039"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(25..1704)
                     /locus_tag="VYS74_001362"
     CDS             complement(25..1704)
                     /locus_tag="VYS74_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3789394.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1706..1939)
                     /locus_tag="VYS74_001363"
     CDS             complement(1706..1939)
                     /locus_tag="VYS74_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV3789395.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(2757..2909)
                     /gene="cibB"
                     /locus_tag="VYS74_001364"
     CDS             complement(2757..2909)
                     /gene="cibB"
                     /locus_tag="VYS74_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV3789396.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2911..3096)
                     /gene="cibA"
                     /locus_tag="VYS74_001365"
     CDS             complement(2911..3096)
                     /gene="cibA"
                     /locus_tag="VYS74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV3789397.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3274..3957
                     /gene="tsaB"
                     /locus_tag="VYS74_001366"
     CDS             3274..3957
                     /gene="tsaB"
                     /locus_tag="VYS74_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV3789398.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QKEGLVVPIMDARRNNVYAGFYENAKPVLPEAHLSFAEVLEQVKDAEQVTFVGEVGAF
                     VEQIQEQLPQASYQETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3954..4391
                     /gene="rimI"
                     /locus_tag="VYS74_001367"
     CDS             3954..4391
                     /gene="rimI"
                     /locus_tag="VYS74_001367"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV3789399.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            4381..5391
                     /gene="tsaD"
                     /locus_tag="VYS74_001368"
     CDS             4381..5391
                     /gene="tsaD"
                     /locus_tag="VYS74_001368"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV3789400.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5431..6737)
                     /locus_tag="VYS74_001369"
                     /pseudo
     CDS             complement(5431..6737)
                     /locus_tag="VYS74_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7013..7364)
                     /locus_tag="VYS74_001370"
                     /pseudo
     CDS             complement(7013..7364)
                     /locus_tag="VYS74_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7471..7764)
                     /locus_tag="VYS74_001371"
                     /pseudo
     CDS             complement(7471..7764)
                     /locus_tag="VYS74_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            8007..9213
                     /locus_tag="VYS74_001372"
                     /pseudo
     CDS             8007..9213
                     /locus_tag="VYS74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            9206..10176
                     /locus_tag="VYS74_001373"
                     /pseudo
     CDS             9206..10176
                     /locus_tag="VYS74_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            10186..11802
                     /locus_tag="VYS74_001374"
     CDS             10186..11802
                     /locus_tag="VYS74_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789401.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            12151..12579
                     /locus_tag="VYS74_001375"
     CDS             12151..12579
                     /locus_tag="VYS74_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789402.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPSTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            12591..13340
                     /locus_tag="VYS74_001376"
     CDS             12591..13340
                     /locus_tag="VYS74_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV3789403.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            13353..14525
                     /locus_tag="VYS74_001377"
     CDS             13353..14525
                     /locus_tag="VYS74_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV3789404.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPFGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            14921..15784
                     /locus_tag="VYS74_001378"
     CDS             14921..15784
                     /locus_tag="VYS74_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3789405.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            16070..16270
                     /locus_tag="VYS74_001379"
     CDS             16070..16270
                     /locus_tag="VYS74_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV3789406.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            16337..17002
                     /locus_tag="VYS74_001380"
     CDS             16337..17002
                     /locus_tag="VYS74_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3789407.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            17074..18300
                     /locus_tag="VYS74_001381"
     CDS             17074..18300
                     /locus_tag="VYS74_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3789408.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTTLYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         5748 a         2982 c         3781 g         5970 t
ORIGIN      
        1 gaagctgacg tggtttgaag agtattattc ttcatctggt gtgaggatca tcgggatgat
       61 gatcggttca cgttcggtat tttcatagag gaaggggcga atagcgttga caatggcacc
      121 attgacagat tgcacgctag catccttatt tttcagtgcg atacgaatgg cattgaagag
      181 gatacgctgg ctttggcgaa tcaagtcgcc agactctctc atgtagacaa agcctcggct
      241 gaggatgtct ggaccagata gaatcatctg cgatttgaag tcaacagttg cgactgccag
      301 aacgacaccg tcttcagata gatcgcgacg atctttgagg acagctgcgc caatttcacc
      361 gatacgattt ccatcgacat agatatcttg ggcgttgaaa tgacctgcga tacgagctga
      421 gtcagcagta agggcaagca catcgccatt gctcatgata aagatattgt ccttctcaac
      481 accagtatcc actgctagtc cagcgtggac tttttgcatg cggtattcac cgtggacagg
      541 catgaagtat tttggcttaa tcaagcagag catgagtttt tgctcttgct gaccaccgtg
      601 tccagatgta tggatattgt tcactttacc gtggataact tcgacaccag cttcagaaat
      661 gatgttaatc agcttgttga cgctagtagt gtttccaggg atgggactag aagagaagat
      721 aacggtatca cctggttgta attgtacttg acggtgggtt ccgttggcga tacgagagag
      781 ggctgccata ggctcaccct gactacctgt acagaggata agaacttctc ctgcaggata
      841 atctttgatt tcatttggct cgataaaggt tcccttagga gctttgatgt agccaagatc
      901 gattccgttg acaatggcct tttccataga acgaccaaag accgcaatct tgcgtccagt
      961 cttaacagca gcttctgttg cctgctggag acggaagata tttgaggcaa aggatgcaaa
     1021 gatgatacgt ccttcaatac cttggataat cttcataatg gactgaccaa cgactttttc
     1081 agagttggta aaggttggta cttccgcatt tgtcgagtca gacaggagac agagcacacc
     1141 ttcttcacca agcgcagcca tacgatgcaa gtccgcaggt tctccaactg gagtaaagtc
     1201 gaacttaaag tcccccgtac agacgatttt cccttgagga gtatgaatga caatccccaa
     1261 aggctctgga atagagtgag tcgttctaaa gaaagttgcc ttgagatttt taaaggtcaa
     1321 ctcggtgttg tggttgattt cgtaaagttt ggcgttgcgc aagaggccgt gttcttcgag
     1381 tttcccacgg atcaaagcca aggcaagcgg tccagcgtag atagggacat ttgcttgctt
     1441 gagtaggaac ggaatcccac caatgtggtc ctcgtgtccg tgtgtgatta aaacagcctt
     1501 gacgcggtcg atattgtcca cgatgtaaga gtagtcagga atgacatagt cgataccaag
     1561 caagtcatct tctgggaatt taatcccagc atcgacgatg ataatctcgt cttggtattc
     1621 aattccgtaa gtgtttttcc cgatttctcc tagaccaccg atggcaaaaa cgccgacttc
     1681 ttcaggttta agagtgtagg ccatattaga actccgtaat ttcgaaggcg ccagtttctt
     1741 tttcgtaatc gagcaatttg tcagacaaga gttcgatata ttcgatattg tactctgggc
     1801 gattttcttc gacaagttgg cgagcagtga tacggccctc aagttctgag ctggcatcga
     1861 tgtctaggta aagcgcgcgt gttgtttcac ggcgtgggct acgttctttt gtttcttgat
     1921 aaaaaacttt gtaaatcata tagttccttt ctatttacag gtcagcttat tccaaacttt
     1981 tagcagatat ttgcttgatt tcattccatt ttgtgtctag attgaccttg attcgttaca
     2041 attatttcaa ttataccaca aaatgaaaaa ttagtaaaag acggaaacga gaaagttagg
     2101 gggcaatttc tttggtaagc gtttacaaaa ataaagaaaa tgtattatgt tgataacgga
     2161 agtgaaattt attgcttgtc ataattccat atagatttta agtcaaggag gttgccctat
     2221 gtataactta tttgcttttg ctatcggagt tcgtttgtta gcttttatcg tttttgttgc
     2281 ttgcgtttat ggaggaaatc ttccatttgc caaactggaa cgatgacaaa ccaagttctt
     2341 gtggaaaatt acctttgtga ctctctactc gctttttctc ctcctcgtgc cctatgtcgt
     2401 ttggtttgta ttttactttg gattaaatgc ttagatgccc tgcttatgct ggggtttttt
     2461 gtttggggat gtagagatag tagatttacg acacgaaaat tccaccccat ctatcaagat
     2521 gaggtggtct ttgcattttt ctaaaatgac atgacaatgg aattttcaaa gcttattatt
     2581 tttatgggag tcattgttct gctcaaaaat agagttgaag cataaaaata aaacaataca
     2641 aacagataaa tcgaatggta aagggatagt agtaatgttc aaatcattga gaatgctgag
     2701 caaactgtaa gagatgaaga caataaaatt tatccttcta aaaatgtgtt tcttatctag
     2761 catgacttac caaacttttt acgaaggtct tttccaatgg tataacctgc cataaataaa
     2821 gcgataccaa cacctacacc aaagccaccc ttgatttctt gcaattcctt attagaaatt
     2881 tcacgatagt tgttcaaatt tttcatcata ttaatctcct cctaaaaaat aaccagctgc
     2941 caaaccagaa cctataagtg ctgttccacc tgcaatagcc ttccaagata ctgctactcc
     3001 aaagataacc aagggagcga gaccgccatc aatatctgat aattcatttt cagataagga
     3061 taaaaattga ttgtcaagaa tgtcaaaatt tgtcattgaa ctttcctcct tttttcttgt
     3121 tcacttttat attcggaaaa gaaaaggaaa agtgagcatt tttttaaatt atttttagtt
     3181 aagtgagggc actcttgtct gggggcaaag aaaattccca tgtgccagct tttgtagtat
     3241 aatagtaagc agacaaaaag ataggaatgt gttatgaaag tattagcttt tgatacgtcc
     3301 agcaaggctc tttctctcgc tattttagag gacaagcagg ttcttgccga gacgacgatt
     3361 aatattaaga aaaatcacag tattactctt atgcctgcca tagatttttt gatggcaagt
     3421 ttggattgga cacccaagga tttggaccga atcgtggtag ctgaagggcc gggtagctat
     3481 acaggcttgc gaattgcggt agcaactgcc aagaccttgg ctcataccct gaacattgag
     3541 ttagttggga tgtctagtct attggctctg gtgccacgcc aaaaagaagg cttggtggtt
     3601 cctatcatgg atgcgcgtcg caacaatgtc tatgcaggct tttatgaaaa tgccaagcca
     3661 gtcttgccag aagcgcatct gtcttttgcg gaagtgctag agcaggtcaa ggatgctgag
     3721 caggtgacct ttgtcggaga agttggagcc tttgtggaac agatccaaga acaactacca
     3781 caagccagct accaagaaac cttgccaaat gcagctaatc tagctctttg ggcttgggac
     3841 aaagaagcag actccttgca cgactttgtg ccaaattacc tcaagcgtgt cgaggctgag
     3901 gaaaactggc tcaagaacca taccgagtct ggcgagtctt acattaaacg cctatgatag
     3961 aaatcaaacg aatccaacag cagcctgatc tagctaaagc catctacgct gttatgatag
     4021 ctgtttaccc agtcagtcct tggacgctgg aacaaatcca agctgacctg ttccaagacc
     4081 agacttggta tgctctggct tatgatgggg cagaagtgat tggctttcta gctgttcagg
     4141 agactctctt tgaagcagaa gtcctgcaaa tcgctgtcaa aggagcctat cagggtaagg
     4201 gcattgcgtc agccttgttt gctcaattgc cgacagataa ggagattttc ctcgaagtca
     4261 gaaagtcaaa tcaacgagcg caagcatttt acaagaaaga aaaaatggcg gtcatcgctg
     4321 atcgaaaggc ctactaccat gacccagtcg aggacgccat tatcatgaag agagaaatag
     4381 atgaaggata gatatatttt agcatttgag acatcctgtg atgagaccag tgtcgccgtc
     4441 ttgaaaaacg acgatgagct cttgtccaat gtcattgcta gtcaaattga gagtcacaaa
     4501 cgttttggtg gcgtagtgcc cgaagtagcc agtcgtcacc atgtcgaggt cattacagcc
     4561 tgtatcgagg aggcattggc agaagcaggg attaccgaag aggatgtgac agctgttgcg
     4621 gttacctacg gaccaggctt ggtcggagcc ttgctagttg gtttgtcagc tgccaaggcc
     4681 tttgcttggg ctcacggact tccactgatt cctgttaatc acatggctgg gcacctcatg
     4741 gcagctcaga gtgtggagcc tttggagttt cccttgctag ccctcttggt tagcggtggg
     4801 cacacagagt tggtctatgt ttcggaggca ggagattaca agattgttgg ggagacacga
     4861 gacgatgcag ttggggaggc ttatgacaag gtcggtcgtg tcatgggctt gacctatcct
     4921 gcaggtcgtg agattgacga gctggctcat caggggcagg atatttatga tttcccccgt
     4981 gccatgatta aggaagataa tctggagttc tccttctcag gtttgaaatc tgcctttatc
     5041 aatcttcatc acaatgccga gcaaaaggga gaaagcctgt ctacagaaga tttgtgtgct
     5101 tccttccaag cagcagttat ggacattctc atggcaaaaa ccaagaaggc tttggagaaa
     5161 tatcctgtta aaaccctagt tgtggcaggt ggtgtggcag ccaataaagg tctcagagaa
     5221 cgcctagcag ccgaaatcac agatgtcaag gttatcatcc cccctctgcg actctgcgga
     5281 gacaatgcag gtatgattgc ctatgccagc gtcagcgagt ggaacaaaga aaacttcgca
     5341 ggctgggacc tcaatgccaa accaagtctt gcctttgata ccatggaata aagagtggct
     5401 ctttgtcaag tgtagtgggt aggcgaaaag ctacaatctg gagagtacga aattcgtact
     5461 ctcttttttg atgttcaaag tcatcaaaat ccgtttttta aaattgttaa agttccggaa
     5521 accaaaggct ttgcgcttga taaccttaat caaattattg gtcgcttcaa gtttagcgtt
     5581 ggaataaggc agcttaagtg cgttgataat cttatctcta tcccttaaaa aagtccaaaa
     5641 gaccgtttga aaaagcggat gaaccgttgg cagttcttgc tcaatcaatc caaagaaatg
     5701 gtcggcattc ttctcttgaa aatgaaagag caggagttga tagagttcgt agtgaacctg
     5761 taatccctca gagtaagaaa gcagcttatc tcgtacttcc cgatgggtta agtgcattcg
     5821 aaacgtgggc tgatagaatc gtttctgaca gagcttgaag ctatcctggt gtagtaaatc
     5881 agtccagtgg actgatttag cccgagccta gaaacgaaaa agcgagggtt ccaaaagcgc
     5941 ttgagcgccc gatactccaa ggattttcgg tcaaattggt tcatgatttg gattcgtact
     6001 cggttcatag ctcggctcag atgttggaca atgtggaagc ggtcaagaac aatcttcgcc
     6061 tttggaaaga gttgcttagc gagccgataa taagggctgt acatgtccat ggtgatgacc
     6121 tcgaccagct cccgaacctc tctctgatag cgttggaaat ggtttcgaat caccgcatga
     6181 gttcgcccgt ctaaaattgc gaggatggat ttggactcaa aatcttgggc aatgaagctc
     6241 attttgctct ttttgaagct atactcatcc caactcataa cttttggcaa cttggtccaa
     6301 tcggtttcaa acttaaattc cctcagtttt cggatgacgg tggaagttga gaccgccaat
     6361 ctgtgggcaa tctcagtcat agactgcttt tcaaggagga gctgagcgat tttttgtctc
     6421 accatgttgg aaatctggca atttttcttg acaatggatg tttgagaaac gaccacctta
     6481 aggcaattct tgcactgaaa gcggcgcttt ttgagatgta gtaccgtggg taaaccctga
     6541 cagtcgagaa gcggaatttt agaggctttc tggaagtcgt atttgatcat cttcccttga
     6601 caatgaggac aaggaggagc gggggaatcc aactttgcct gaacgactag atgggtttgg
     6661 tatttcagaa cagacaagat tttgatattt ttgtctttca atccgagcaa atctgtggta
     6721 ttcttaagtt gttccatatg aaccctttct aatgagttgg ttagacgctt ttcattatag
     6781 gtcatatggg gctttttttc tacaagaaac gaccctataa ttcctggggt gggattaccc
     6841 actacagaaa ttatagagcc aaaacattcc aaagtcttgt ctgactctgg aatgttttct
     6901 gttcacagtt gttttgtaac tgcctaataa gtaaccgcgc tataacgagg tatctaccga
     6961 gattgtcaat tctccagtat actgttataa aaaacaaaac tggaggattt ttttatgtcc
     7021 ccccatcgtc ccatcgactg ttccgtagct gcccaataac ggagtatagt cggctgaaac
     7081 aagaatagga caaaagtgcc tcaagaaaag catcgtaatt tgacaatatt attctgagat
     7141 gtttttgatg taagcccata ttttctcaat gggattatac tcgggtgaat aggtaggaag
     7201 tggtaacagt ctatgccctg ctccttacac atgttcattc tgtgaaacct tgcattgtcc
     7261 ataatgataa gggatggtgt atctaaagta ggtagtaaga atttttggaa gcaagcttcg
     7321 aaaaagtcac tcgtcatcgt atctttatag gtcatcggag ccatagtata ttgaaacaaa
     7381 aaagggcatt agtacttcta taacagtatc gcaatctctg gtgtgcgttg tctcgatctt
     7441 aattcaaata attatatctt cttccagaga gactaactgc ctgctcaagg ctcgatcata
     7501 ttcgagataa aaataggtct caacccctgt ctcataaata taaataggag tcaggtagct
     7561 taagtgattc aattctttaa ggaaccgatt tactttttct gggtcttgtt cgcagtaggt
     7621 acagctcttt tttagactat atcccatagc ctttagagcg taatgaatag ttgttggagg
     7681 acagttgaat tcagcagcta tttcagtcaa ataagcatcg ggatgagttt caatatagat
     7741 ctttaattta tctctatcaa ctttttcaat taatagtgga agttttctta aagttagtgt
     7801 gttgaaacgt ttttatacag tgctaaaatt tgaagtgtag tgcagatgta tttgtaatag
     7861 tattatagca attctttgga gaaggtcgag acaagagaaa ataggtatct ttgagatata
     7921 agcatgattt actataaaat tattgagtta ttcataaaaa tttctctatc aaatatcata
     7981 atgcgtcgtt taaggagatg gttacaatgc aatatagttg tggaaaaata aatattaata
     8041 tccctgatgg atatggagac attaaggata tagtgttttc ggctcatatt atagttaggt
     8101 ataataatgg acattgtgga ggtatagatc cgcatataat tggactttgt aaaaagcaga
     8161 tcagaaggat gtctctatat cctattttga taatcgtatc tagagattca aaggttattg
     8221 atgattataa aaatttagat attgcctatg ttgattgtac tcaatgttca aataattttg
     8281 aaactgcctt acacgttaaa aatattttaa aattattaaa aatccaactt atacactgtc
     8341 atggctactc aactaattat tttttatata tgttaaaaaa acttgacaag aatggatttg
     8401 gaaaggttaa gacagtgata acatgccatg gatgggttga atataactta aaaaagaaat
     8461 tcttgactta ttttgatttt tggacatatt ctatgggaga tgcttttatt tgtgtatctg
     8521 aaactatgaa aaaaagattg gagagtataa taaaaaaata agaagatagt tgcaataaat
     8581 aacggaatta atgtatcaaa tagcgattta gatgtagtag gtgttcaaga ctttaaaaaa
     8641 gaattttgta ttccaaataa caaaaaaatc atttgttatg ttggaaggtt ggatccagaa
     8701 aaaaggccgg atagattcct tgaatttgca gaaaaattat ttttagtcag agaagatgtg
     8761 atttttatta tggctggaaa tggtagtatg tgggctgctt tgaaagaaaa aatttgccat
     8821 ttgaaatgta gagataattt caggttactt ggtgaaatct atccagctac tatagtttac
     8881 caaatatcgg atttattgta tattccttct gatacagaag gaatacctat gtgtgtttta
     8941 gagtcaatgt cacaaggaac tccggtttta gctagtaatg ttggcgggtt aagtgaaatt
     9001 attgaacata gggttgatgg atttttattt gagaaggaag atgttgaggg agtgtgtgct
     9061 tgtgctaatt ttttactcaa tgattctgag tatttgaaat atataggtga gaatagtaaa
     9121 tcaaaaataa gaaaacattt ttctgtgcaa aaaatgtttg tagaaaccat gagagtatat
     9181 gatgaattat tagagaagag tagtcatgga taggaatata gatcaagaat tagtaagtat
     9241 tataatacca acacataata ggtatgaatc tctgattaga gctgtgaaaa gttgtttaca
     9301 tcaaagttat aaaaatatcg aagttataaa aatatcgaag ttataattat tgatgataat
     9361 tatagtaatg taaatttaag aaataaaatt atccatcaat ttggctatac caatcataga
     9421 attaagttaa ttttaagtaa tgaagattta ggtgcaacta atgccagaaa cataggtatc
     9481 aaaaattcta gaggtaagta tatatcattt ttagacgatg atgatgaata tatgtcagat
     9541 cgaattttaa agttgatggc ttgttttaaa aagagtagaa tgaagaattt agctttagtt
     9601 tatagttatg gaataataat ttatccaaat ggtacacgag aagaggagaa gaccgatttt
     9661 gttggaaatc ccttgtttgt tcaaatggtt cacaatatag caggtacgtc attttggttg
     9721 tgtaaaaaag aggtgctaga attaattaat ggttttgaga aaatagattc acatcaggac
     9781 ggtgttgttt tattaaaact acttgctcaa ggataccaaa ttgatatagt gcgagaattc
     9841 ttggtgaatt actacgctca cagtaaagaa aacggtatca ctggagtgac acaaaaaaca
     9901 attaatgcag atgaagaata ttataattac tgtaggaaat attttaattt attgagtttc
     9961 aacgagagaa tattggttac aaagaaatat tattctttaa acataaagcg gttactatta
    10021 ataggagaca aatgcaaggc tttaaaagta atcaagaagg caagagaaga aaaaattttt
    10081 aacgaatttc tttttttgaa atatatgtta ttatatcatt ttggtttttt ctattgtata
    10141 tatgacaact atgttcaatt aaaatttaga aagtgagaaa ctattgtgta tactattata
    10201 aaatcaaata taaaaaaatt tagtttatta acgatattta ttgttgctgg tcaattattg
    10261 ctaatttatg cagcaactat taatgctctg gtgttgaatg aattaattgc gatgaattta
    10321 gagcggtttt tgaaattgtc aatctaccaa atgattgtct ggtgtgggat aatattcctt
    10381 gactgggtag tgaaaaatta tcaggttgaa gtgatccaag agtttaatct agagattcga
    10441 aatagagttg ccacagacat ctctaactct acctatcaag aatttcatag taaatcatca
    10501 ggaacatatc tttcgtggct aaataatgat gttcagactt taaatgatca ggcttttaaa
    10561 caactttttt tagtaataaa aggaatttct ggtactatat ttgcagttgt gactcttaat
    10621 cactatcatt ggtcattgac tgtagccacc ttgttttcat taatgattat gctacttgta
    10681 ccaaaaatct ttgcatcgaa aatgcgagaa gttagtctaa atttaactaa ccaaaatgaa
    10741 gcttttttaa aatctagtga gactatattg aatggatttg atgtgttagc atccttgaat
    10801 cttttatatg tattgcctaa gaaaattaaa gaagcaggaa ttttattaaa gatggttata
    10861 caaagaaaga caactgtaga aacgttagca ggcgctatta gcttctttct caatattttt
    10921 tttcagatat ctctcgtttt tttaacaggc tatcttgcaa taaaaggaat agtgaaaatt
    10981 ggtactattg aagcaatagg agcactaaca ggtgttattt ttacagcgct aggtgaatta
    11041 ggaggtcaat tatcctctat tattggtacg aagcctattt ttttaaaatt gtattcaatt
    11101 aatccaattg agtcaaataa aatgaatgat atcgaaccaa atgaggtgaa tagagatttt
    11161 ccgttatatg aagcaaaaaa tatttgctat aagtatggag ataaagaaat attaaaaaac
    11221 ttaaattttt gttttcaacg taatgaaaag tatttaattt taggtgaaag tggaagcggg
    11281 aaatctacat tattaaaatt attgaatggc tttttgagag attatagtgg agaattgcga
    11341 ttctgcgggg atgatataaa aaaaacctcc tatttaaata tggtttcgaa tgttctatat
    11401 gtagatcaaa aagcttattt gtttgaaggt acgattagag ataatatttt attggaagaa
    11461 aattatactg atgaagaaat actacagtct ttagagcaag ttggtttgag tgtaaaagat
    11521 tttcctaata acattttaga ttattatgtt ggtgatgatg ggagattact gtcaggaggg
    11581 cagaaacaaa aaattacttt agctagaggg ctaattagaa ataagaaaat agtattaatt
    11641 gacgagggaa cttctgctat cgataggaga acttcgttag cgattgaacg taagatatta
    11701 gatagagagg atttgactgt cattattgtt acccatgctc cgcatccgga acttaaacaa
    11761 tattttacta agatatatca atttccaaag gattttattt aagaatatag gggagggaaa
    11821 catgagattc tattgtgaag cttatgaggt aattactgaa gagaaaaaag tatactttaa
    11881 tgatattgag ttagaagtta agtattcctc tgtcgaagaa ttgtttataa tatgtgagaa
    11941 attattagat aagaaaaaag tatcattttt ttatgtagat gagaaaccct taaggtatct
    12001 gttatttgac tatatatttc tactagttct agctaaaaaa atatcccaat cttatgaact
    12061 gcaccccaaa agttagattt attctgtcta acttttgggg tgcagttcat tttgcatagt
    12121 tttggcattc atcctagata tccatataaa atgtctgaga ttgttgaaat gtctaaaaaa
    12181 atatcagatc taaacgataa aaaagaattt cacaaaaaga actgtgagtt gaggtttcaa
    12241 gatagtgttc ttgataacat aaatagtgtc ccgagtacaa ctcatttatt tagttgttta
    12301 aatattcaaa cattagtaaa aattatttta tatatttttc gatacaacag ttgtatgaaa
    12361 atatttaatt ttagagataa agagcttatc ttttttaata agattgtaaa tgggctgata
    12421 caaaatattg aggagaattt agaggatgat attgaaagga tattgaaata tttatatata
    12481 tgtttattta acgaaatatt cattattaag aataaggtga atttttttga tgatgtggaa
    12541 tttaatcaga cacttagtga gtttttagat aaattgtaga gggaatggtt atgagtaaca
    12601 atactaagta tatcttttta tctccacatt tagatgacgc aattttttcc tgtggtgatt
    12661 atatttcaaa attaacttca gaaggagaaa ttgtacttgt tattactata ttttctggtt
    12721 accctttgag ccaacagcta cagccctctg ctaagcaatt tcataagttg tgtaaccttg
    12781 ggaaatatcc gattgaagaa aggaagaaag aagacaggtt agcttgtgaa cgtctacagt
    12841 gtgattttag acacttatcg tactatgaat gtctctatag gaaagataga aatggcaatt
    12901 ttctataccg tcatatctat agtgaattaa aaaatgagga cacattaaag aatgatatca
    12961 taaaagagct gttaatgcat ttagacgaca aatgtgtcgt atattgtccg ctttcacttg
    13021 gtgaccatgt tgatcatgtt tttgtgaata gtattggaag ggcgcttgaa tttatgcgtt
    13081 ataaagtaat atattatgag gacttcccat atgtaagtga tagtagtatg gttagttata
    13141 tgggaaaaac taaagaatta aaaatgtatc aagaggaact tgatgaaaaa cattatattg
    13201 atagaatatc atcaatattg tgctataaat ctcagatttt gataatctgg aaatcagttg
    13261 aaaaactttt aaacaatatt aaagagttgt atttaaggaa tggggcagct tattctatta
    13321 gattttggat taagaaataa ggaagtagtt ttatgaatat agcagtaatt ggtttggggc
    13381 atgttgggct ggcctacgcg ttactatttg catctaaata taaagttgtt gcatatgata
    13441 tagattctgt aaaaataaat aatttaaaaa agggcattct tccatctaaa aatgaagagc
    13501 ttatgaagtt tttttgcgag aataacttaa atattacttt ttttgataca ttttctgaaa
    13561 ttaaaaataa tattgattat tatattattg cgcttccgac agattatgat gagaaaattg
    13621 gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa gattctgagg gtaaaaccta
    13681 atggaaagat tattttaaag tcaacagttc cgttcggctt ttcaaacaaa ttaaaaaggc
    13741 tgtttgatac aaaaaatatc atttttgtcc ctgaattttt gagagaaggt tgttctatat
    13801 atgataattt atatccaagt cgcatagttg ttggagatga gacagttgaa ggaagaaaaa
    13861 ttgcagagtt gttcctttcg attagtactc atagtactgc caatattaaa aatgttatgt
    13921 tagtttctcc tactgaagca gaagcaatta agcttttttc taacacattc ttagctctcc
    13981 gtgttgcttt ttttaatgaa ctagattctt ttgctgagag gagaagttta aatgctgaag
    14041 ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa tttttataat aatctttctt
    14101 ttggatttgg aggatattgt cttcccaaag atactaaaca attaaaaaaa gaatttatag
    14161 aaataaatgc cccagtgata gaagcgattg atatttcaaa cacaaataga aaacagttca
    14221 tagttaaaca aatattggaa cgaaagccaa agatagtagg aatatataaa ttggggatga
    14281 aatataattc agataattac aaagagtcag ctattttaag tataattaat gaactattga
    14341 ttgtcggtat aaaaatttta gtatatgaac caaatttaaa cgttagtata gataacgtaa
    14401 tttttgaaaa gaattttgaa ctatttacaa aacaaagtga tctaattgtt gcaaatagat
    14461 gggatagggg ccttgaagct tataaagata aagtttacac aagggggatc tggataagag
    14521 attaactatt gatattaaaa aggcaataat ttataaaatt tgtacaaaaa aatgttaaat
    14581 tttcacattt tcatagtata ctgtttaata gaaagtaggt tctttgtaac cgctccatta
    14641 taaaatcctt tcactcttcc ggtatactat taggaaaaac ataactggag gatattttga
    14701 gctcacacct catcgttcca gtggctgttc cccaagctcg tgtactgccc aaaaaagtta
    14761 gacattctat ttagaacgag gattgagttc tgtattgtac aaggctcggt ccttttagag
    14821 tcagcttaag gctggctttt ttattttttc taactatcag aatattttga caaatgaaca
    14881 caaataatga tataatatgc aaaagctagg aggtggtagg atgattgaaa aaatggaatt
    14941 gggggaattt tacaaggaat tacgtttggc gagaaagctc aagcagtcag atgtggcttg
    15001 tgacgggcta acagcttctc agttgtccaa gtttgagcta gggcagtcca tgttgtctgc
    15061 tgataagctc atattagcca tccaagggat caatgtgacc tttgatgagt ttgggcacaa
    15121 gctcaacaac tatcaagaat ctccacatat gcagattggt agaaaggttg tggatcgctt
    15181 tgctcatcaa gatatagctg gcttagagca actgttggag gaagtcgagc aagaacagat
    15241 ggcagagact tatcgtcgtt tgaatgctat tgtaatcaaa gatgccatcc actcactaga
    15301 taaaagctac ccactagcag aggaggatag cgagtttttg accacctatc tctatgctat
    15361 cgagtcttgg acctggtttg aactctatct cttttgcaat actctgccct tcttgagcaa
    15421 tcaagatctg atctttttat caacctcctt actcgaaaaa tccaaagaat ttaaagagtt
    15481 agtacacaat cgattgtata tgaagcaagg actcttaaat atcatctcag agctcatgga
    15541 gcgcaaactt ttttcttata tcccaatctt tgaagccgag ctggagagta tgctccgtcc
    15601 atacgatgtt tttgagaaag tgttgtggca gtttttaaag aagatgagca ttttccttca
    15661 aactaaggga agcaatcaaa aagagattga acactttatc caatctctgc aagtattaga
    15721 aaatccacaa ttaatagccc tttttgaatt gcgtttgcag caatacaaaa agcttatcga
    15781 ttagttagaa atggcaaaat aataataatc tcagcaacga ttttcaataa tgttaaaatt
    15841 tgtttcttca tatttctcca ctcctcatta gtagtaactt tattatacca gagaagtaat
    15901 caaattcacc aaaatcgcaa atctgaaatt tctataagaa aaaatatcaa atatgcgatt
    15961 ttctaaaata agccaatttt cgtgttatac tgtacttgta aagcacttga agcaaatcct
    16021 aggtcgcaga agtggtttac aaatgaagat aattgaagga gtgtaaaaga tgttaaattt
    16081 acaatttgca gaaacaatgg aattgacaga agctgagttg gagatagttt atggagggga
    16141 atttgggaat aatgctgtta tcccagccgg tgcttgggga gggttcggga caccttggtc
    16201 aatcactaat ttctggaaga aaaattttaa cgatagacct gactttgatt ctgaccgccg
    16261 tcgttattaa tatccaaagc ccttgccttt tcttgggtag gggctgtctt tatttcatag
    16321 tataggagga aatagaatga aaaagataat ctcacaccgc tactttatta tagtttttct
    16381 actagttatt gctgaccaga agttcagtgt tctagtttta cgcagcgacc ttgttactgg
    16441 gctatctgac tttgcctatt atctgtcgga tatgatgttg aattttcttg tggttttatt
    16501 tgctcttatt gctatgattt ggtcggggaa atggcagaaa atcaatagta gaaagtttaa
    16561 aggatcctat cttttctatt ccttcctagc tctgcttgct tttgttgttt ggaatttcgt
    16621 tacctttttt cttttcccac ctactcgaaa tgaaatttct tatcaacatg ctgctccgac
    16681 ttttacaggg gctacagcat ttttgatgta ttttttctat cctgtgattg caggtcccat
    16741 ttttgaagag ataatccatc ggggattggt gatgacagct ctggaaaaag gaaagaaatg
    16801 ggggctagat gtacttggct ctgctatttt atttggggtc tcgcacatta gtaatcacgg
    16861 ttgggtcttg acagactttg tcttctatat gggtggaggt cttatatttg cagtcttatt
    16921 tagaatgaca aagtcaattt attggcctat tggactgcat atagtctaca atggtattgg
    16981 tcagcttttg atgttactgt aattttggtt gttagtagta tcgcggtcta ttcttgggga
    17041 aagattccca tttgaactta agaaggagtt gtaatgaaag tatttcttca aaatagagat
    17101 tttaggcaat taactatcaa ccagtggatt tcaacgcttg gggatacgat tttttatctg
    17161 gccttcctga attatgtggc agatgcgtct tttgcccctt tggcgatttt acttatcacg
    17221 atttcagaaa cccttcccca agttctacaa atctttctgg gagttttggc ggattttcaa
    17281 catcatcgtg tcttgaaata cacagtcatt agttttacaa aatttttgct ttactctata
    17341 gtgtccctat cgctttcagg gcagtctttt tccttgttgc tagtaacctt tatttgtatg
    17401 attaacctct tgtctgacac attgagttat ttttcaggcg ccatgctcac tccgattttc
    17461 attagaatta ttgggcaaga tcatctggca gaagctattg gatttaaaca gtcaactgtt
    17521 agtttagtga aaacaatcag taatatccta ggaggagtct tactaggtat tctatccatc
    17581 cagtttattt ccttactgaa cgctcttacc tttttaatcg catttttagg tatcctcttc
    17641 ataaaaactg acctcttgaa agtagaaaaa acgattagct atcaagaagg actctctgta
    17701 aaatcctttt gccagcattt gctccaatca tcgaaattga tatggaatat gaataaggtg
    17761 ctcttggttt tgtttattat ctctactagt caagcagtga taaatgttac agttcctgtc
    17821 tctactctat ttttgaggaa tcaacccttt ttgaatttac aaacaggtca atctcttgcc
    17881 ttgctatcca cttttgaact gtcagccctg attgtcggaa gccttgtaag tggctatctg
    17941 caacatacca tttccataaa aacaactctc tatgcctcac ttgtcatcca gttgcttctt
    18001 ctagtaggat ttgccacagt tcgttttgac tggattctca tctttagtac cttggatgcc
    18061 tttttcgcag gtgttctctc tcctagattg caagaactcg tctttaaaca aatacctgag
    18121 gagtcaatgg gagcagttca atcttctatc agtgccatta cggttgtttt acctagctta
    18181 tttacaatat ctttggtaac cattgctact agctttggaa ctctggcagt tagctttgtt
    18241 ttattgctat ttcttctagt tgcctttgtt atgcttctga atattcgtga aagtatttag
    18301 tggagaattt ctataatata tgtttgagac tggattgcca gtctttttat gatggtcaaa
    18361 atcaacagac tttttctctt ttctgaatgt gttatagtag actgaatcta aaatagtacg
    18421 aaacaattgc taaaacattt atagaaatta attttacttt cccaatcgat ttgttctcat
    18481 c
//
LOCUS       DAVMMR010000040        18463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMMR010000040 DAVMMR010000000
VERSION     DAVMMR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.040"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..787)
                     /gene="lytB"
                     /locus_tag="VYS74_001382"
     CDS             complement(<1..787)
                     /gene="lytB"
                     /locus_tag="VYS74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV3789409.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLK"
     gene            complement(829..1767)
                     /locus_tag="VYS74_001383"
     CDS             complement(829..1767)
                     /locus_tag="VYS74_001383"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV3789410.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1778..2578)
                     /locus_tag="VYS74_001384"
     CDS             complement(1778..2578)
                     /locus_tag="VYS74_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV3789411.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2828..3208)
                     /locus_tag="VYS74_001385"
     CDS             complement(2828..3208)
                     /locus_tag="VYS74_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3789412.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3266..3625)
                     /gene="rplT"
                     /locus_tag="VYS74_001386"
     CDS             complement(3266..3625)
                     /gene="rplT"
                     /locus_tag="VYS74_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV3789413.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3677..3877)
                     /gene="rpmI"
                     /locus_tag="VYS74_001387"
     CDS             complement(3677..3877)
                     /gene="rpmI"
                     /locus_tag="VYS74_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV3789414.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3910..4440)
                     /gene="infC"
                     /locus_tag="VYS74_001388"
     CDS             complement(3910..4440)
                     /gene="infC"
                     /locus_tag="VYS74_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV3789415.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4456..4587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4747..5928)
                     /locus_tag="VYS74_001389"
     CDS             complement(4747..5928)
                     /locus_tag="VYS74_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789416.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVVVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     gene            complement(5932..6519)
                     /locus_tag="VYS74_001390"
     CDS             complement(5932..6519)
                     /locus_tag="VYS74_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789417.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6551..6775)
                     /locus_tag="VYS74_001391"
     CDS             complement(6551..6775)
                     /locus_tag="VYS74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789418.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            complement(6917..9157)
                     /locus_tag="VYS74_001392"
     CDS             complement(6917..9157)
                     /locus_tag="VYS74_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV3789419.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNTW
                     KIESVR"
     gene            complement(9141..9791)
                     /locus_tag="VYS74_001393"
     CDS             complement(9141..9791)
                     /locus_tag="VYS74_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV3789420.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            complement(9859..10428)
                     /locus_tag="VYS74_001394"
     CDS             complement(9859..10428)
                     /locus_tag="VYS74_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789421.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10605..11030
                     /locus_tag="VYS74_001395"
     CDS             10605..11030
                     /locus_tag="VYS74_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV3789422.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            11031..11444
                     /locus_tag="VYS74_001396"
     CDS             11031..11444
                     /locus_tag="VYS74_001396"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV3789423.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            11390..11716
                     /locus_tag="VYS74_001397"
     CDS             11390..11716
                     /locus_tag="VYS74_001397"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789424.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(12083..12343)
                     /locus_tag="VYS74_001398"
                     /pseudo
     CDS             complement(12083..12343)
                     /locus_tag="VYS74_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12346..12846)
                     /locus_tag="VYS74_001399"
     CDS             complement(12346..12846)
                     /locus_tag="VYS74_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789425.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            12882..13061
                     /locus_tag="VYS74_001400"
     CDS             12882..13061
                     /locus_tag="VYS74_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789426.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(13039..14007)
                     /locus_tag="VYS74_001401"
     CDS             complement(13039..14007)
                     /locus_tag="VYS74_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV3789427.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14093..14308)
                     /locus_tag="VYS74_001402"
     CDS             complement(14093..14308)
                     /locus_tag="VYS74_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV3789428.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14317..15171)
                     /gene="cvfB"
                     /locus_tag="VYS74_001403"
     CDS             complement(14317..15171)
                     /gene="cvfB"
                     /locus_tag="VYS74_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV3789429.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(15231..15788)
                     /gene="frr"
                     /locus_tag="VYS74_001404"
     CDS             complement(15231..15788)
                     /gene="frr"
                     /locus_tag="VYS74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV3789430.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15797..16534)
                     /locus_tag="VYS74_001405"
     CDS             complement(15797..16534)
                     /locus_tag="VYS74_001405"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV3789431.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16618..17952)
                     /gene="trmFO"
                     /locus_tag="VYS74_001406"
     CDS             complement(16618..17952)
                     /gene="trmFO"
                     /locus_tag="VYS74_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV3789432.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(18247..18441)
                     /locus_tag="VYS74_001407"
     CDS             complement(18247..18441)
                     /locus_tag="VYS74_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3789433.1"
                     /translation="MEAEAIRRNCERGAIFSGKIKHHEDSQFKGDHYVECYAVLDNTV
                     IARDRITVPIDPLCGKDFIE"
BASE COUNT         5307 a         4192 c         3416 g         5548 t
ORIGIN      
        1 acttgaggta aaaccaagat tccttatccc aaatccattc attggctgcc atgtagccac
       61 cggattttag ataataatag tgaccattct cgaaaatcca ttctttatca gcatagtttc
      121 cattttcttt tatgtaaaac caagattggt attgtttgtc aaaaagccaa ccttgctgta
      181 ctttggcacc actagcattc acataagctt gattaatcca ctgacttgtc agtagataac
      241 caccggattt gaaataatag tccttccctt taatttggag ccattctttc tctgcgtgct
      301 gtccatctgc tttgatataa aaccaagcat cgtattgaga atcatagacc cagtcttcta
      361 ttactttggc acctgttgca ccaacatagg aagttcctac ccaagcattt cgtttcatct
      421 ttccatcttg gtcaagataa taaaaggctc ccttgtcttc tacccattct gatttggcca
      481 tatagccacc agatttgagg taaaaatagt cgtcaccttg ctttagccat tcattttcag
      541 catagttagc atctgctttt atatagaacc aagattgata atgagtatca aaaacccact
      601 cattcgctgc ttgactgcca tcttctttca gatactgttt tccttgccaa gtaccatcac
      661 tagccattgc accctctgga ctagctaaga aaaatagcag agctaaaaaa ataaatctta
      721 ctttcttcat taaccttctt cctctgttct tatttatttt attgtaccaa aattctccta
      781 aattcatatt acaaactaat aaaaaatcaa gaacagattg attgcagttt acctcagaga
      841 ctcttttact tcctgacgga gttcttccag actgctaata ccgtatttat ccatgacttt
      901 tggtaaattt tcgatgatgt cagggcaggc ataaggattg gtaaagttag ctgttccaac
      961 tccgatagca gatgccccag ccagatacat ttctagggca gcttcagccg aatccactcc
     1021 ccccattcca atgataggca ggtctgttgt ttgggctact tggcggatga gtttgagggc
     1081 tactggaaag actgctggac ctgacattcc acctgttcca ttggccaaga ttggttttct
     1141 ggttttgagg tcaaagcgca ttccaaccag agtattgatc atagtcaagc cacttgctcc
     1201 cgcatcttct gcagcttttg cgacagtaac gatatcggtc acactcgggg ttaatttgac
     1261 ataaactggc acttctgagg cttccacagc tgctttcacc acatcataag ccaaatctgg
     1321 atcttgacca atcaaaagtc catgattaca gtggtcaaca ttgggacaag aaatattgag
     1381 ctcgatagct tttacattag ttgccttgga aatcccatga gaaacagctg catactcttg
     1441 ttttgaaaaa ccagctacat tggcaataat aggaagattt ggatattctc tttccagcca
     1501 aggtagcttt tcagccaaaa caacctctaa accaggattt tgcaagccaa ttgcattgag
     1561 cataccagca ggcgtctctg ccactcttgg agttggattc ccaaaacgtg gttcaagggt
     1621 tgtcgccttg atcataatag aacctaaaag gtctaaatca tagtacttgg catactcttg
     1681 tccaaagcca aaacagcctg atgctggaat aatcggattt ttcaaatcca aaccaggtag
     1741 agacacttgt aatcgatttg tagtcataat tttctcctta taatacaact gttcctgtgc
     1801 ggaaaacagg accatcttca cagacgcgtt ggctgaccgt ctcgctttct ggtactttta
     1861 gaacacaggc atagcaagct cccatcccac aagccatacg agattccaga gataaatagg
     1921 ctcttgggtg atcatcaaag gtttgattga tatacttcat cattcctgga gccccacacg
     1981 agtaaacagc atcaaactga ctatctaaat cattgataac aacggaaaca tttcccttga
     2041 tgccataaga accatcatct gtcgttacaa agacctgacc atactgagcc aattccgttt
     2101 tcaaaataac agcatcctta ttagcaaaac cgaggactgt cactactttc actccacgtt
     2161 catgcaattc cttggccacc tcaagcaagg gtggaacacc aatcccacca ccaacaagga
     2221 gaacctgatt ctgctcatca aggtcagaca agtcaaaacc atttccctga ggccccatca
     2281 catcaagagt gtctccctga cttaaggttg aaaaaattgc agtcccagct ccctcaatcc
     2341 gataaatgag gtgacactgc ttgtttgcct tgtcaataga cgaaattgaa ataggacgac
     2401 gtaagagatg ggcatcgtct ggtacacgca gatgaagaaa ttggcctgct cgcatggctt
     2461 caaccatttc tccttctagg actaattcaa agattgctgg cgcgatttcc tcttgtgcaa
     2521 ccaccttcat ggtttccaac cgaatgacac ccaaacgctt cttacatgtg agattcataa
     2581 cttttcctcc ttaaatttta aggggaccag ataaagaaaa gaccttgcta gtgcaaggcc
     2641 tcagttaaaa tcgtacaaca caagagagca cactcaaaag gtctcctcta gaaaattgca
     2701 ctattctttt atctgacacc ttgtgagtct ctctggactc cccttaaagg ttgaattttt
     2761 attagtatac tctttcagct aaaaaaagtc aagtagaaaa cgaatattct acttaacttg
     2821 cacgaaatta tttttcacga atgacttcga ccttatatcc atcagggtct ttaacaaagt
     2881 aatagtttgg tgtagttcct ggtagaccat ttggctcagt cacttcatag ccttttgcac
     2941 tgtgttcttg atgaagtgcc tcaagatcag gtgtactgag ggcgatatgg gcaaacccat
     3001 caccaaccac atacggaccg tgatcgtagt tataagtcaa ctccaactca taatcatcac
     3061 cctcaagacc tagatagaca atcgtgaagg catggtctgg aaaatctctg cgacgcaatt
     3121 ctttaaaacc aaaagcatct tgataaaatg cgattgattt ttcaagattt tctactcgta
     3181 agcaagtgtg tagcattttt gaagccatat ctttctcctt tatttttaaa aagactggac
     3241 aatcctgttc cagtctcatc agttgttatt taccaagttt tgctttagct gcatctgcaa
     3301 gagctgtgaa agctactgca tcgttaacag ccaagtcagc aagcatttta cggttaactt
     3361 caatctcagc caatttcaaa ccatgcatca attgtgagta tgaaagtccg ttcatacgag
     3421 ctgccgcatt gatacgagta atccacaatt tgcggaagtc acgttttttc tgacgacggt
     3481 cacggtatgc atagtagtaa gagttcatta cttgttcttt tgcagtacgg aacaagatgt
     3541 gtttagctcc atagtaacct tttgctaatt taagaatacg tttacgacgt ttgcgtgata
     3601 caacgccacc tttaacacgt gccatgtttt atttcctcca aatatttcct agaattgttt
     3661 acttacagtc aagctattat ttcaagcgag taagcattgc tttgatacgt ttgtaatctc
     3721 ctgaatgcac catagatgct ttacgaagat gacgacgttg tttcttagtt tttccgtgga
     3781 aacggtgaga agtgtaagca cggaaacgtt taagtccacc agaacctgta cgtttgaaac
     3841 gtttagctga tgcgcggtgt gttttttgtt ttggcatgat tttttctcct ttatttaact
     3901 ttctgacaat tattttttgt cagtcgctgg cgccaattgc atgaacattt gacgtccatc
     3961 cattttagca cgttgttcga tgatggcaat atcttgagtt gcttcagcaa actcggctaa
     4021 aacttttgca ccaatctctt tatgggtaat catacgaccc ttaaagcgaa tagatacctt
     4081 aactttattt cctttttcaa ggaacttacg tgcattacga agttttgtat caaagtcacc
     4141 cttgtcaata gttggactta gacgaacttc tttcacagta acaacgcttt gttttttacg
     4201 ttgttctttt tgcttcttct ggtactcaaa tttgaactta ccgtagtcca taatttttgc
     4261 aacaggcggt ttggcttggg gttgaatcaa tactaggtca acattagcgt tatcagccaa
     4321 agcttgcgct tcactgagtg gcttgatacc tagctgttct ccttcaagac caatcaagcg
     4381 aacttcacgt acacgaatct catcattgat gaataagtct tgctttgcta tggttttcac
     4441 ctcttttgtt ttattagaga aaaacaatag cggacttgta aatatacaag cccgcacgtt
     4501 atgatagcgt ttcttcgaaa cttttcatcc gtagggccag gcaacttaat gtcacaaggc
     4561 gagaagctct cacttctgct tttctcaact tttatatgat accaagtttt ctagctcttg
     4621 tcaagataaa aagttatttt ttgaaaagtt tgtgtttgta ttttggtttg gttttcgaat
     4681 caaaaagaga gaaccaagtt gattctctcc ctatgttagt ttttctttag cttccctata
     4741 ggaaaactaa actttaaaaa cttccttttt acgtgaactt ccccagcttt cgataaatag
     4801 aatccccact aacaaaagga taatcaggca aggaagtaag accatcccca tgctccaatt
     4861 tagatcgaca ttatcaatct gcttagataa tcttccagat aaaatctcca ctgaacgcaa
     4921 gtaagtaaag ggaatcagat gtgcaatcct ttgaagaggc tgaattgttt ggatgccaaa
     4981 caataagcca acaatcccaa tgagtgaaag aaagaggaca ggcatttttt gcttgaaaaa
     5041 gtaagcaatc aagtagacga cttccacaat gacgataaag gctaagaaag ctaagagcaa
     5101 gccaggaaat aatacatctt gtattttccc aatagttact tcttgattca ctaagctata
     5161 aattgggtag ggataatcta actgtccaaa accacttatc agacttccca ctagaaaaga
     5221 aaagccacag attccgataa acagcacagt tacatatccc actacaactc caagagagga
     5281 tattgcaaat gtcacttttg aaacaggata taagtgagct gtgtccagat gattttgata
     5341 tctttctgca aatagttgtg ttagcataaa aataatcgca accacaaaca agcttgggat
     5401 gataacctct aaaatccaga caatctgatc aatcccgtgg gtcggaaact ccaaagtatg
     5461 tgcttttatg ttcaagggat acagggcttg gtaaatcttc cgttcgcggt caacccccat
     5521 ttttaagcca gagctagcag tcgggtcatt tgatacaaat tcataattct tctcttcatc
     5581 ttgccactgc agatagtagg cttctttcca gcgcccttct tttagtaaag ttagaatttc
     5641 tgtctttcgc gtcaaaagat ttttttgcac gtctaaatta tttttagcaa actggtattc
     5701 ctccgagctg gtatcagaca tttgggagag tttctcttca ttttcattga tagccctctc
     5761 gttggctgca atgcgacttt ccaacctgct ctccaagctg tgtgagtttg cagtctgact
     5821 atttaaataa aaggtaacac cgagtacaga tgcaaataaa agtaagataa tccagtttaa
     5881 acgacttttg aaaacttttt tcaataaaaa tagactaaca tctttcataa actaaacctc
     5941 ttctatctgc ccctgatgaa tggttactac tctatcgcag atatcaacca actcttcctt
     6001 atagtgggaa cttaaaagaa ccagctgttc ttgtctatcg atttgtgcta gcctatcaaa
     6061 aaacttctgt cgataatact cgtctaagcc atttgtaatc tcatccatga gccagcattt
     6121 ggcctgactg agaaaataca tagcaatcac caagcgttgc ttcatgccta aggaatactt
     6181 gcggatggga agactgatat agtcagccat ttcccagtag gcgatttcat ctctcaagtt
     6241 taggtctgac ttccagatgt tttttatgag acgaaggtag tccatcccac ttaagtttcc
     6301 atccagccat tcaacgctct cataataaaa caaagaagga ggaactgcga tgtgtccact
     6361 actaagggga agcaacttgc tcatagctcg gaatagtgtc gtctttcccg agccattgat
     6421 agcaagaaga ccataaaccc tacccttttt aaaggtaaaa tccgcatctt gcaagatgac
     6481 ttgtcgcgtt tttagggtaa catgagtaag ccttaacata tccagccctc cttttctcac
     6541 tctttaagga ttaataacct ccagtatagt agtttatgac ctcataacga gcgtagttcc
     6601 agccagaacc aactggatac tcagaatagc tgtaataacg agaccatgac ggaatccaag
     6661 catactgatg gtcgtgatag ttagtactat attcccaaac cgtattccaa tcatacttgt
     6721 aacttttagt gactgtcaca gcagatatac tggactgaaa aagaccaata atcataaact
     6781 aactaattaa acgatttttg ctaattttta atgattttat atcctcagta ttgaatacgt
     6841 tttcattata ccatttttat cattattatg caacaaattt ttagtttatt tcactaatct
     6901 acaacattta tctttcctat cgaacacttt cgattttcca agtattccaa cctttaaacc
     6961 gtatagctcc ttgctggtca gttcggtaaa ctttgctatt gataccttcc agtcgtgtca
     7021 atgtttcctg atgggggagt ttcattcgat tgctctttcc aactgagata agagtaagct
     7081 ctggtttgag tttttctaga aaggctggac ttgatgattt tttattgcca tgttggctag
     7141 ctttcaaaac atttactttc aagtctggat agtgcttcag caagtccttc tctcctttct
     7201 cctccaaatt tcccgtgaag agaaattgct tatccaagaa tttcccatac agaactaggg
     7261 tatcatcgtg tcctccatct cccattttcc ttggggatag aacttctaac tgacttccaa
     7321 aaatgggcaa gttctcccct actgtcatac tacgcacctt tgtttgagtc gcctgtagtt
     7381 ctaccacaaa ttgcttctgc ttcaaactgc cttttgatac taaaatctcc cctacatgga
     7441 aagccttggt cacctctaac aaatctccga catgttcctt gtccgtgtta gtcaaaatta
     7501 gctggtcaat cttagctact cctcgacttt tgagataggg aatcaaggtt cgctgggcat
     7561 tgctggtcgt cattttttct tgccattttt cgattttctt ataagattct gccttaccac
     7621 ctacatctat gagaatggtt ttcccagtta catcccgtag gaaaatactt tctccttgcc
     7681 ccacatccag catggtgatt tcattttcca gtggatgctt ggtcaggaga aagagccctg
     7741 taatcaataa gcacaatacc gttagctttt taatgttttt tctcaaatca tagaccaaag
     7801 ccaaggaaat taacaatagg attaaaaacc atgcattggg ttgtccaaag accagaggtc
     7861 tacttgtcac ctgtgacacc aagcgaataa tgccctccaa ccattcaaag ataaagttca
     7921 gctgaatgac tggatagaga aaggaaagga caaataaaat agacaagagc ggtaagaagg
     7981 tcaagtcaaa tagaaaggaa aagacaaagg tcaaaaggat agacaaaggt tgaaattccg
     8041 caaaatagaa ggatagaatg ggcaatatgc ccaaggagat gactagactt tcactagtaa
     8101 cagccttgag cccctcccct tctttgctgg tcatggtcag gataaaagca taagcgcagg
     8161 acaagactcc tcctgctgtc aagaaaaagt ttggcatgac aataaagagg acaagcaccg
     8221 tcaaggcaaa attatccaag cccttaaccc catgttgagc cagtagcttt tgcaagagac
     8281 tgcgaataac cgatgctgaa aatccagtta gtcccgcata gataagggaa aagggataag
     8341 tcagccattt caacttttct tgggtcaagc ccaatcgcaa gagaagtttc ttaaatccat
     8401 tcatgaaaaa acctacctgc ataccagata gggcaaagag gtggataatt cctagactgg
     8461 aataaagctc attcatctcc tcaaagtcgg tgtccagatg tcccagcaag agtcctgtca
     8521 tgtaattgcg catagggtct ggaaagtgcg tcttaatcca aaccacagcc tttcgacgta
     8581 aactggacaa gttttctcct atatcccaac tgccaatctt ttgaagtgac tggatttttt
     8641 tgatattgag agtctggtaa attccctgag tcttcagata ggcttggtaa ttaaagccac
     8701 caaaatttct ctgcccttct ggctccgaaa gcttcccttc tagtcctatc tcatgcaggt
     8761 cggttaaagc ttgaaaggct tctttctcct cctcggactg gagtttataa tagacttgga
     8821 aagcacgacc gttagacttg ccacgaaagg atagactatc accattaacc ttaatagtat
     8881 caggcaaaat ccgtaccctt tcaacagaat ccgccagatt ttgactcgct tgactctgtt
     8941 gccaattttg aaaaacaaac caaaatccaa agattccgca aattattaga actttaccag
     9001 cagatttcca cggaaattgg ataaagagac agactagcag aaaaacaaag cccaacaaag
     9061 caagataaga tgctgagaaa atagcgtaat aaagccaaag taatagaaaa ctcaggtaaa
     9121 ttaggggaat agagaaattc ttaatccact gtaacatagt ctttaagctt ttctattgtt
     9181 ttgccaccaa tgccagagac cttcttgagc tcgtctactg acttgaactt gccatttgcc
     9241 tcacgatggt cgataatgtc ctgagctcgt tttcctccca gtcccttgac ctgcttgagt
     9301 tcttccagac tggccttgtt gagattgacc ttcttttcct tgcttattga agaagctgtc
     9361 cccgaaccag tctgttgact aactgcttct tctcccttag taggaacgta aaccagagcc
     9421 tcatcactaa ctttctgagc tagattgagc gacttgctgt ctgcttgctc tgtcaagcca
     9481 ccagccttct gaacagcatc attgactcga ctacctacag gcaagtcata aatccctggc
     9541 gatttgacag cacctttgac atctactgtg attagatctt gttcaagggg ttcttccttc
     9601 tcttccttgt tcacttcctt ttcggatacc aaatccttgg aaacagctgc aacttcagcc
     9661 tgcaaattcg tttcctttac aggtgtttgt ggagctggct ttagcaggaa aaatccgcct
     9721 acaagcaagc ccagaccagt acagatgacg atgattttat actctttgat tttctcgata
     9781 attgcttcca tattttctcc tctcttagat tattcgtaag aggaagaaaa aacagtcgaa
     9841 aaatttcttt tcaactgctt atttatttgc aaaatagtct tccttggtca agacataatg
     9901 aactcttgtc acgattcggc ctttttcatg ctggtccata caagcatatg gttctgcatg
     9961 ggaaaaacgc atgcctgatt tctccatgac ctttcctgac gcagaattgt ccttatcgta
    10021 taaagcaact aacttattca ttcctatctt ctcaaaagct agctcaatca cagcacgatt
    10081 ggtttctggc gttaatcctt gattccaata ctttttattg ataatgtagc caatagctgc
    10141 cttcttaaga acaggatcaa tcttgtgtaa gtcaatagtt ccgataaact tgccgttatc
    10201 ttttaattca atcccccaac gtcccaaggg attagccaag tagaactgag caatgttatt
    10261 cttggtttct tccaagcttt gattggttgg aaaagtgtaa cgtgtattac ccttgtccga
    10321 ggcatagtca aacattgctt ccgcatcatc caaagttaca ggtctaagca ataaacgctc
    10381 cgtttctata gacggatact gggcaaattt cacaaatatt gattccatac ttttccctcc
    10441 attagagctt gattttcact agtctagcat aaaaaaattt ggatgacaag tttttcttga
    10501 tttttgaagc ggtttcatat acaatgaaac cgtcacattt ttgatttatg aactggaatg
    10561 tgacaaccaa aacaagaccg tcaattgaaa ggtaggttat ctcaatgtta atcggaatcc
    10621 caaaagaaat taaaaataac gaaaaccgtg tcgccctcac acctgcaggt gttcatagct
    10681 tagttagtcg tggtcatcgt gtccttatcg aaacaaatgc tggtctcggt tctggcttta
    10741 ctgatgctga ctatcaaaag caaggagctg agattgtcgc tactgctggt gaagcttggg
    10801 cagcagagtt ggttgtgaaa gtaaaagaac ctttaagttc tgaatacggt tacttgcgcg
    10861 acgatcttct tctcttcacc tacttgcaca tggccgctgc tccagaatta gcagatgcta
    10921 tgttagcagc aaaaacaaca ggaattgcct atgaaactgt tcgtgacaat caaggacaac
    10981 taccgctcct cgttcctatg agtgaggttg caggtcgtat ggctgtttaa atcggagctc
    11041 acttccttac taagcaagct ggtggctctg gtgttctacc tggtggtgta ccaggtgttc
    11101 caaaaggaaa agtaactatc atcggtgatg gtgtcgtcgg tacacatgct gcccgcatcg
    11161 cccttggtct tggtgctcaa gtgactattt tagatattag tgccaagcgt ctctcagttc
    11221 tagaagaagt ctttggaagt caaattcaaa ctcttatatc taattcattc aacattgaag
    11281 caagtatgag agatgctgat gtggtgattg gagccattct catccctggt gcaaaagcac
    11341 cggaattggt gacagatgag atggtcaaac aaatgcgtcc aggctctgta tcgttgacgt
    11401 tgctgttgac caaggtggcg ttatcgaaac agctgaccgt gtgacaacgc acgatgaacc
    11461 cgtctatgaa aaacacggtg ttctccacta tgccgttgcc aatatccctg gtgcggttgc
    11521 ccgcacttca accatcgccc taaccaatgt cactcttcct tatatcgaag ctttggctgg
    11581 caaaggattc gcacaagcaa tctctgaaga tgaaggcttg cgtcaaggtg tgaccactta
    11641 tcaaggttac ttgactagcc taccagttgc tcaaggactt aatcgtgact acactgatat
    11701 caatgattta gtataaattt tgaaaagaaa agggtgactt cgcaagtcct acccactgtg
    11761 ctttgtgtca tcagctatta catcgttggt ttattgtact gtatactttg caactggaga
    11821 ctttcgttcc agactcaatt gtgcataaga aactctaaat tgaataaagt caacagagtt
    11881 tcgcccaacc acttcgtctt gtaatgacaa ttatataaac caaaggaagc tgagctcttt
    11941 agtcccagct tctttttata tataaaattt tacccgtgca aagacagggc cttagcagac
    12001 ttctttttta cttcgttcac ccttgctttt tctttttagg tttgggcgtt ggcagttggt
    12061 tatacatagc taaaatcagg tcttatagaa acatcttatt atcaagttct tccactcaaa
    12121 tcatttcttt ggcacctttg tatggaaact caaaagaaga ttggtcaatc ttatctaaga
    12181 ctgcttgcac gggtttaact aaaaagcgat cgtcataaat gccgccaata atcttgccgc
    12241 ggaagtaaag aatatactcc ccaatcatgg aacggtaagt cacatcatct aatcctgata
    12301 attgttccaa aacaaattcc aaatagttct tacttgatgc catttctaat cttctaggct
    12361 ctgttcagcg ataacaaccg tatagagttc ttgcttaacc tcgcatccaa ttgatttaaa
    12421 gccctgcttt tcccaaaaat gctgagattg cggatttccc ttaacataag ccaaacgtgc
    12481 ctttcgaaag ttcttagcaa aataagctag tgcttctgtc acaatatgac taccaatccc
    12541 tttcctctga taggcttgat caaccataaa caaaccaata aaaacagtct cctcatcagg
    12601 atgtgcatag acaaaatcta taacagccac aaggtcagat ccattccaaa atccaacaaa
    12661 aaacttatca gccttagctt taccttcagg tagacaaagc atgtcctctt ttacagttgc
    12721 aaaatttggc tctggtggac aatgctgaaa atacagagga ttactttcat ataaagataa
    12781 aatacttgga atatcctttt cagttagtat cctacaactg taatacttag atagttggtc
    12841 aatcatcttt tcaaattcct actttcttgt gccctgtgat tatgacacag gaagatgcac
    12901 tgatcgtcat cagccacata aaagttcttt ccatcgtgcc taatcgttgt ctcaaacctt
    12961 tggataaaac ctttagccta tacaactgga ttttcctctc tcaaaagtat attcttttgc
    13021 aggcaaactt cctcaaaatc agtcgtgtgc aacttcagta gaatattcat aggctcggat
    13081 aatctgagcg acaactggat ggcgaaccac atccttggct gaaaaatgaa caaagtcaat
    13141 ctgatggatg ttcttgagtt tctcttgagc atcaatcaaa ccggacttga cattacgtgg
    13201 caggtcaatc tgactaatat ctccattgac aatcatctta gaatgaaaac ctaaacgagt
    13261 caagaacatc ttcatctgca tgatggtcgt attttgcgcc tcatcgagaa tgacaaaggc
    13321 atcatccaag gtccgtccac gcatataggc aaggggcgca atctcgataa tttcacgctc
    13381 catgagtcga gttgtttggt ctttccccag aatctgatac aaggcatcgt aaacaggacg
    13441 aaggtaagga tccaccttct ccttaagatc acccggaaga aatccaagac tctctcccgc
    13501 ttccaccgct ggacgagtta ggataattcg cttgacttgc ccacgtttaa gggcagtcac
    13561 tgccaaggtc actgcaagga aggtcttccc tgtacctgct ggcccaattc caaaggtcac
    13621 atcatgctgt ttgacactgt ccacataaag cttttgccct agggttttga cacggatagg
    13681 tttcccagta ttatctttga taatttcttc ttcgtaaagg gcgacaaact tgtcaatttc
    13741 atcatttttg accatgctaa tcgcagtgac tacatctggc gtaccaacgg tcatcccacg
    13801 atttaccaag accatcaaag cctgaataac ttgacgggct tcctcacagg cagactcttc
    13861 tcccaaaacc tggacaatct ccgtacgagc atgaatcaca acatcaagct cttcttccat
    13921 caaacgaaga tggcgttcat tggaaccaaa aagatgaaac aggtcatctg gatgactcag
    13981 ttgaatgtct attgaatgtt ccttcaaata aagaacctct ctaatccttt tatttctttt
    14041 tattatagca gagggatgga gaaattacta gcccaaaccc aatgaataaa tgctagtgtt
    14101 ctagatattc ctgccaaatg ctgtcaaaat ctcctaggtt aaaagagaaa ctggcatgct
    14161 cctccaaaaa gcgactgacc tcatcaaaat catctgtatg ttttggaaaa gctgcctctt
    14221 caaaagcgag gtctgccaaa attgctttgg gactgttact tttaggattg cgctcggtca
    14281 tgagccaagt gtaaaatgat tttctcataa gcctccctaa atcaactctg tcccaaactg
    14341 gtcctgcttg attttaccag ccttcataag accacctaaa gctttcttga actgaccttt
    14401 agaaatgcca aaggttgcct tgatgtcgtc tggagatgac ttatcattta aggtcatgaa
    14461 accgccatta ctttccaaat aagtcaaaat catctgagca tcgttttcca acatttcaaa
    14521 ggagcgtggt ttgagggaga ggttcagagt gcggtccact tcacggaaac caataacgcg
    14581 cgcatctaat acttgcccca aacgtggctc tgcgtaacgc tcgctaggat gaataaaacc
    14641 aagcatatta ttttctggta ggtaaacaaa agttcctgac agcttgagac ggtaaacaat
    14701 ggctggccag ttttggttct gcatgttgtt gtaggcagga cgagcgagac gttggaagtc
    14761 ttcttgataa gccaagaggc cccagatacg gtctttctta tccacttcaa gacggatgta
    14821 gagttggtcg cccttcttag gccagagttc cttgagcaca gggagaatat cgagtgacac
    14881 aacgatttcc ttgtcaggaa ggcctgtatc cacaaagaca cccaagtcct tacgaacctc
    14941 tgtgacacgt ccccaaccaa attggtcctg agtggcagtc acttctaagg ttgtcaggcg
    15001 gagtttttgc ttcatatccg tgtatgcaaa acctttgacc gtatccccta ctgtatgttg
    15061 gccctcttcc ttagcaagag cataggtttg accatccttt tgcacaaagt aaaaacggtc
    15121 attttcatcg atgatcagtc caacgataaa acttgcaaga tttgtattca tatttccttc
    15181 tttcgaataa aactcagcca gcaatgccaa ctgagttttt ctgtttattt ttagacttcc
    15241 aaaagttctt tctctttgtt agcagtcatg tcgtcgatgt gtttaacagc atcgtctgtt
    15301 actttttgaa tatctttttc aagagtcttc aattcgtctt cagtgatttc ttttgctttt
    15361 tcttgcttct tagcttcgtc catagcatcg cgacggatat tgcggacagc cactttagca
    15421 ttttcgccga ccttcttcac ttctttagca aggtcacgac gagtttcttc tgtaagagct
    15481 gggataacca agcgaatcac agaaccgtca ttagccggtg tgataccaag atcagaagcg
    15541 ttcaaggcac gttcgatgtc tttcaatgaa gacttgtcaa atggtgttac caacaaaaca
    15601 cgcgcttctg gaatcgtaat tgaagcgatt tggttaagag gagtttcgac tccatagtat
    15661 tctacatgta cacggtcaag caagcttgca ttggcacgac cagcacggat accaccaaat
    15721 tcacgagcaa gtgattggtg agactgggtc attctctctt tagctttttc aataattacg
    15781 ttagccatat tctttcttat tccttttctt cgatattatt tgaaactgtt gttccgatat
    15841 tttcaccaaa tacaacacgt ttgatgttgc ctgattggtt catgttgaag acaaccaagt
    15901 caatgtcgtt gtccattgag agggttgagg ctgttgagtc catgatacga agacctttgt
    15961 tgataacatc acggtgggtc aattcttcaa acttaacggc tgtcttgtcc ttcttaggat
    16021 cggcattgta cacaccatcg acgccatttt tagccatgag gatggcatct gcttcgattt
    16081 cagctgcacg aagggccgct gttgtatctg tcgagaagta tggtgaacca attccagcac
    16141 caaagataac gatacggcct ttttcaaggt gacgaagggc acgtccacgg acataaggct
    16201 ctgccacttg ttgcatagca atagctgttt gtacacgcgt atcaacccca acttgttgca
    16261 atgaatctgc catcacaaga gcattcataa cagtcccaag cattccagtg taatctgcct
    16321 gaacacggtc catacctgct tctgctgcag gttctccacg ccagagattt cctccaccaa
    16381 taacaagggc aatttcgata cctaagctat gaacttcttg aatctctttt gcgattgttt
    16441 gaactgtttg gatatcaatc cctacgccac gttcaccggc aagggcttca cctgataact
    16501 tgattaaaat acgtttatac ttgggattcg ccattttcac tctccttctt tcatcctacc
    16561 tattttatca caatttctaa gatttttata gtatcatgaa caattctttc aaaaaaatta
    16621 gacagtcaaa aattcctcta agtcggcaag ggcacgctct gcaatttttt cataacgagc
    16681 cttcttatca cggatacgct cgccttccaa ctccttgatg atcccaaaat tgacattcat
    16741 tggttggaaa tgtttgctgt cggcatgggt aatgtaatga gctaagcttc caatcgctgt
    16801 cgtctcgggg aaaataacct cgctttcttc cttgaagaga cgagctgcgt taattcccgc
    16861 aactaagccc gaagccgccg actcaacata gccttccacc cccgtcatct gaccagcaaa
    16921 gaagagattt ggttgtttct tggaacggta agtctgctca agaagatttg gtgaatccat
    16981 gtaagaattg cgatgcatga caccataacg gacaaactcc gcattttcaa gacccggaat
    17041 catttggaag acacgctttt gttctcccca tttgaggtgg gtctggaaac caacaatatt
    17101 gtataggcta ccagctgcat tatcctgacg aagttgcaca accgcataag gtgttttaaa
    17161 ttctccatca cgaggtcctg tgtagtcgtc cggatactca agaccgactg gcttcatagg
    17221 gccataaagc atagttttaa tgccacgttt ggccataact tcgattggca tacatccttc
    17281 aaagtacttt tctttttcaa aagaattgag cggtgcttct tccgcattga ccaaggcttc
    17341 atggaaatcc ataaactctt gcttggtcat tggagcattg agataggctg cttctccctt
    17401 atcataacga gatttgagat agaccttact catatcgata gtgttgacat cgataatagg
    17461 cgctgctgca tcgtagaaat agaagccatc gccattatta agggcatgaa tcttttcagc
    17521 cagggcatcg ctagtcaagg gaccagtcgc cacaactgta ataacatctg ttggcaattc
    17581 tgtaatttca tcacgcacca cttcaatcaa ggggtggttg accacttttt cggtcaccat
    17641 ttgagagaaa ccatcacgat ccaccgcaag ggctccaccg gcaggaacac gtgtagcctc
    17701 agcagattcc aagataacag aacccaagcg acgcatttct tccttgagaa gaccaactgc
    17761 atttgtcaaa gcatccccac gcaaagaatt ggaacaaacc aactcagcaa aattgtctgt
    17821 tttatgctgg ggtgtagact tgacaccacg catttcataa agtttaactg gaataccacg
    17881 ctctgcgatt tggtaagctg cttcagaacc tgccaaacca gcaccgataa cattgatata
    17941 agattgagac acgacactaa tacctctttg ggagtgtgaa gttaagattc acattgaaaa
    18001 agccaatcag acttacgaga tttcgagttt cttggctcag gctgaaaaag tcccccagac
    18061 tttcctcgct ctcgaatttc ttggctcaag ctgaaaaagt cccccggact ttttcactcc
    18121 cacaaatctt tctatttttt tcttctacta gtataacaaa aaaagggaag aaggcaaact
    18181 tccctgttta gtcattttct tgatgtaaag agatagagag tattccagtt aagaatcaat
    18241 tatatgctac tctataaaat cttttccaca taacggatcg atagggactg ttattctatc
    18301 tcttgctata accgtattat ctaaaacagc ataacattca acatagtggt ctcctttaaa
    18361 ctgagaatct tcgtgatgtt ttattttacc tgaaaaaata gcaccacgct cacaatttct
    18421 tctaatcgcc tcagcttcaa tatttcttac tttccaatag act
//
LOCUS       DAVMMR010000041        17989 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMMR010000041 DAVMMR010000000
VERSION     DAVMMR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.041"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VYS74_001408"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VYS74_001408"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV3789434.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VYS74_001409"
     CDS             complement(863..2125)
                     /locus_tag="VYS74_001409"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV3789435.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VYS74_001410"
     CDS             complement(2135..3265)
                     /locus_tag="VYS74_001410"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV3789436.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VYS74_001411"
                     /gene_synonym="pce"
     CDS             complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VYS74_001411"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV3789437.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(5231..6118)
                     /locus_tag="VYS74_001412"
     CDS             complement(5231..6118)
                     /locus_tag="VYS74_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3789438.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VYS74_001413"
     CDS             complement(6108..6569)
                     /locus_tag="VYS74_001413"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV3789439.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VYS74_001414"
     CDS             complement(6566..7474)
                     /locus_tag="VYS74_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3789440.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VYS74_001415"
     CDS             7467..7664
                     /locus_tag="VYS74_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789441.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VYS74_001416"
     CDS             complement(7716..8387)
                     /locus_tag="VYS74_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3789442.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VYS74_001417"
     CDS             complement(8441..8662)
                     /locus_tag="VYS74_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV3789443.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VYS74_001418"
     CDS             8934..9743
                     /locus_tag="VYS74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV3789444.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKALADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VYS74_001419"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VYS74_001419"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV3789445.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VYS74_001420"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VYS74_001420"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV3789446.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VYS74_001421"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VYS74_001421"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV3789447.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VYS74_001422"
     CDS             complement(13018..14277)
                     /locus_tag="VYS74_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV3789448.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VYS74_001423"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VYS74_001423"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV3789449.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            15390..16105
                     /locus_tag="VYS74_001424"
                     /pseudo
     CDS             15390..16105
                     /locus_tag="VYS74_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(16071..17546)
                     /locus_tag="VYS74_001425"
     CDS             complement(16071..17546)
                     /locus_tag="VYS74_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV3789450.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         5295 a         4141 c         3268 g         5285 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcaa ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gatctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctgatatt gagaaagcag ttcagaaaac tgagcaggct
      661 taactcctag aaaaatcaca tctgcttctg caaacatttc ttcattgctg gaagcctgac
      721 caccaaagtc tgcaatgaaa gcatctacct tagcttgact acgattggca aggagaatct
      781 catctgacgt cctagtctgc aagacagatt ttgccaagct agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc tcttactccc ttatctgccc atcaccagca accacatact
      901 tgtagctggt caactctttc aagcccattg gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatagc atccgaatga tgggtactgt gggattcaat atgcgcaacc gcttcttcta
     1141 aactgctcac aaccttaaca gctagaatat agtctaaaaa ctcggtatca aagtcttgtg
     1201 cttgagcagc ttgacctgaa acaaactggc ttgctttgct atctaggcgg aattgaattg
     1261 gttccaaccc agcttctttt cgatctgcaa ccaacatttg atccaagcga ggaaggatgc
     1321 ttgctgcctt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtc cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgag
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttatcaaat actgtcacga tatcaccgta agaaaagact ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 tcatggaatc tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca
     3001 aaagtccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tacccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactactg ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtctttaatc
     3661 caacctatag ctcgtgaacc atctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcatgatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc actgctcttt atcttgaatc
     3961 caaccaattt ccatctggtt ttctttgtta aaataatacc agattccagc gattttcttc
     4021 caatttttag cagcaacacc agagtctgtc aaatagaacc aacgattgtt ccattttttc
     4081 cattgattct gtaataagat acccgtttgg ttaaagtaat accattcacc cttaatttca
     4141 ttccaaccga cagcatattc tcctgtagaa tcaggcgctt gataccacca gtttccatat
     4201 gaactcttat gccaaccagc ttgaaaactt ggaatcggct tgtaggaagt tgaaatgttt
     4261 actaaaccgt cttgtcgaat atcaaaaact gttgcatcat agtctttgct ggctgcattg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa
     4561 attttcttgc cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt
     4621 ttctttaatt cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg
     4681 tccccaaact gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt
     4741 tctgcagcag tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa
     4801 ttagtaatac gactatcact atatttctta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga tagcgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 aatggccgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct
     5521 tccctttagc agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc tttgagttct tgggcaagta ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat
     5761 tgatacccga caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat
     5821 gaccagcact cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg
     6061 acaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactgaccc tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag
     6301 accatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacaactgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttggggatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata agatttgacc aactgatcca gcccaattaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattttttta gtaagctaac ttcctgaaaa taggagtaac
     7741 caaacatccc agaaggcagt gatattgatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa atcagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctatcgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg
     8401 ctttctccac ttgtttcttc atattactaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattgcct cctccaatat atcctaaatc ataggtgcct ctggaacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg ccaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataataatag caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gacggcaccc tcctcaatga agccaaggaa atcccacaag
     9001 ctcacattac tgctattcac aaagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact ggagcttgac ctccagaacg
     9121 aatatattat tgttaacaac ggttgttcaa ctcaccagac cagtgattgg agtctagttg
     9181 actggaaaga actgagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtggc aaacccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgaag
     9361 aagcgactag tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccgc ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaagc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga caagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt tagacgttca cttgctccct tgtctaggtc ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat tggctctgaa
    10261 ccgatagctg taggataaaa gagcaattca gcaccattca atgcaagaca gcgggctgtt
    10321 tcagggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct
    10441 ggtatatggg tctttcgata aacgcccagc actcccccat ctgcatcaat gacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa tcaccttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgctcttc ttctccttct
    11041 tcatagatgt agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaacc
    11221 tcagcaggac caacaaaggc aacaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaggtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gcctttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat taaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgctc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcact cggaatcttg tctatagtaa ctccaaagcg agaaccaggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacga gccccatgct tacgcaactg tctctctgag ttaaagacga
    12721 tatggtctgt tatcccaagc aattcctcca tgtcctcatc cttgaaagct ggtgcaaaaa
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaccatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaacgcct gtcgtgtatg aaattgcttg
    13201 tgacccgact tctgcatagc actcttgatg gtcgcaaaca ttgtagatgt agatagtctt
    13261 ttcaacgcca tctttgacac ctgtaaagat acatccaata ttggttttac ctacagtacg
    13321 tgggccaagg ctagcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacctggcg cagaaacctc acggagatta atttctggat taaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacacta gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 agcttctggt ttgtagcttt caatcagggc aatcacttct tcaaccttgt cagcatcaag
    14101 tgctgcagtt tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaat gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggatcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccaacc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tatatttagc gcagttagct actgccaaag
    15181 cccaagtggt atacttggaa taagccactg tggattagtt tattccctcc aatcgcttga
    15241 cgatttccga aaggctctaa ccacagtttt ttgtttcaat tggtaatgag tttgataacg
    15301 catagacgtt ttatctcgtc taaggtaaca aggcaaaaaa ttcgtggata accaagtaga
    15361 cttgaaatac tacatctgga ggtactccca tgctttatgt gggcattgat gtcgctaaaa
    15421 ataaacacga tgttacagcc ttgaatgttc caggaaaaac tgttcttaaa ccactcactt
    15481 tttcaaataa taaagctggt tttgaactct tagatctgtc tcttcgacaa ctcaaccaag
    15541 actgtctcat cgctcttgaa aataccggac attatgcctt taatctactg aacttcctgc
    15601 atgaacaagg atataaagtt tacacctaca atcctttgct catcaaggaa tttgccaaat
    15661 cattatcact tcacaagact aaaacggata agaaagacgc tcatggcatc gctcttaaac
    15721 ttctttctga ccccaatcgt gaacaatttc aacacgataa tcggcaagta gaactaaaaa
    15781 tactggctag acatattcat cgtctcaaga aaaacagtct gattggaaag tacaatacac
    15841 tcgttgtctt gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata
    15901 tacctaccaa ctcttgacac gctaccctaa tcctcagaaa aggattgagg caggatttga
    15961 taagctgata gaaattaagc gattgaccgc ttctaattca agggcttttt gccgtgctct
    16021 aaaatcatcg ccaaatcatc aaattgaaat aagaaatagt ttagataaaa ctattgactt
    16081 ttcttatagt ttgtctttct cttaataacg ttgatatgat tgacctctgg atcttccgtc
    16141 ccttggaggg agcaaccacg ttccttggcg aattggatat agtctacaat ttctcgtgta
    16201 atgcgttcac caggagccaa gataggaatc cctggaggat agcacatgac aaattctcca
    16261 cagacctgtc caacagactc atctaaggtc aaactttttc tctctgaata gaaggcttct
    16321 tgtggagaca gcaccaactc gggctgaata tattctcctg ctatcaaatc ttttccatct
    16381 cttgaataga gtctcttaat atcagccaga gcaccaacca agcgctcgat gtcttggatg
    16441 cggtcgccga tggaaatata ggccaagata ttgccgatat caccaaactc gatctgaatg
    16501 tcgtattcgt ctcgcaagag gtcataaacc tcgatacctg ttaagccaat accctgagtg
    16561 taaactgaca gcttagttac gtcaaaatcg caaaccgaaa caccgtctat taactctttt
    16621 gagtaggcat agtagccacc gatagcattg atttcacggc gggcatactc agatagctca
    16681 atgactttct caaacgactc tttaccacga agggccaagt tgcgacgtga aatatccaaa
    16741 ctagccatca acaagtaaga ggcagatgta gactgagtca ggttaattat ctgacgaacg
    16801 tattcagaat tcatctgctc cccgattaaa agaagcgaac tttgtgtcaa actcccacca
    16861 gacttatgca tggaaactgc tgccatatca gctcctgcat ccatagcaga aattggaagt
    16921 ttaccagtga aatgcaaatg cgctccatgg gcttcatcta ctaaaaccat catgccagct
    16981 tcatgagcca tttctgtcaa cccctttagg tctgaacaga tgccgtagta agtaggattg
    17041 ttgattagga tagccttagc atctggatgg tcctttatgg cctgtgctac tcggtcattt
    17101 tcaagaccta aagcgatacc aatcttagga tctacactca tctcgatata gatgggaatg
    17161 gcaccacata gaaccaacgc attgatagca gatttatgga catttcgtgg cagaataatc
    17221 ttatctcctg ccttgcaggt tgccagaatc atagtctgca ccgatgaagt tgttccacca
    17281 atcattagaa aggcatggct agctccaaaa gcatctgcag ccagctcctc tgcatcacga
    17341 ataatcgaaa taggatggcc taaattatcc aagggtttca tcgaattgac atcaatgcct
    17401 acacattttt ctcctaagag ttcgacaagt tctggatttc cccgtccacg cttgtgacct
    17461 ggaacatcaa agggaacaat ccttttcttg cgtaacttca ccaaggcctc ataaattggg
    17521 gcttggtttt gatctaactc tttcaaaaca tactcctttc aatattttta ggaccttatg
    17581 aaaactaagg aactaaaggt tgactcatga aaaaagagca ggttttcacc tgctctgcaa
    17641 tcttctgacg tgtttatttt ggtttctgtt gagtatgtct gttcccgatg tttcctaact
    17701 ctctagtagc aaatattacg tactttattg atcgtttcac aagatgacgt gtgctttcac
    17761 cacactaaaa atttatgaat taggacaagt gtcctaacat caacttataa aagtaggctt
    17821 ttaaccctat caataatgtg gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg
    17881 taggcatttt ttactcgggt ttatatttgc tagaaaacat catctcattt tctaagcttt
    17941 attactatat catgttttta agaacttgta aagtattttt tgataaaaa
//
LOCUS       DAVMMR010000042        17694 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMMR010000042 DAVMMR010000000
VERSION     DAVMMR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.042"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(163..1098)
                     /locus_tag="VYS74_001426"
     CDS             complement(163..1098)
                     /locus_tag="VYS74_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV3789451.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1095..1955)
                     /locus_tag="VYS74_001427"
     CDS             complement(1095..1955)
                     /locus_tag="VYS74_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3789452.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2063..4225
                     /locus_tag="VYS74_001428"
     CDS             2063..4225
                     /locus_tag="VYS74_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV3789453.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4317..5576
                     /locus_tag="VYS74_001429"
     CDS             4317..5576
                     /locus_tag="VYS74_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3789454.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5590..6456
                     /locus_tag="VYS74_001430"
     CDS             5590..6456
                     /locus_tag="VYS74_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3789455.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6470..7303
                     /locus_tag="VYS74_001431"
     CDS             6470..7303
                     /locus_tag="VYS74_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3789456.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7304..7384
                     /locus_tag="VYS74_001432"
                     /pseudo
     CDS             7304..7384
                     /locus_tag="VYS74_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7661..9103
                     /gene="gtfA"
                     /locus_tag="VYS74_001433"
     CDS             7661..9103
                     /gene="gtfA"
                     /locus_tag="VYS74_001433"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV3789457.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVTF"
     gene            9241..10434
                     /locus_tag="VYS74_001434"
     CDS             9241..10434
                     /locus_tag="VYS74_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV3789458.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11121..13100
                     /locus_tag="VYS74_001435"
     CDS             11121..13100
                     /locus_tag="VYS74_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3789459.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13167..14663
                     /locus_tag="VYS74_001436"
     CDS             13167..14663
                     /locus_tag="VYS74_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789460.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14663..15589
                     /locus_tag="VYS74_001437"
     CDS             14663..15589
                     /locus_tag="VYS74_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789461.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15598..16665
                     /locus_tag="VYS74_001438"
     CDS             15598..16665
                     /locus_tag="VYS74_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789462.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16676..17602
                     /locus_tag="VYS74_001439"
     CDS             16676..17602
                     /locus_tag="VYS74_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789463.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5213 a         3155 c         3707 g         5619 t
ORIGIN      
        1 aaatgtaatt gttccatatg aatctttcta atgagttgtt tgatcgcttt tcattataga
       61 tcttatggga ctttttttct acacaaaaat aggctccata atatccatag gggatttacc
      121 cactacaaat attatagagc cttatattat aattgtgtta ttttacttcc aactattgag
      181 agaaatttcg ccactattta gccagatttc ttttccgtta tcacattgaa ctaaaagttt
      241 tccattttct gagatgtctt tagcaagtcc cttgtagtct ttttgctgta gtgtgaaagt
      301 gacttctttt cctagaatga atgactgttt tttgtatagg tataatagct cttctgctgg
      361 tgtttcgaag aaagcacgcc agatttctat gatcaattca ttccttgtta taggagctgt
      421 agctttaaat aagctggcag ctttttcttt taattcctga gggaagtctt taatagtgaa
      481 attgatacct actccaataa tgatatctgt gactaagcca gtttctacag aggtcattgc
      541 ttcagtaagg attcctccaa ttttatgatt gtttagatag atatcattga cccattttat
      601 gtcgacatct attaaagtta ggttcttaat agctttgtag acagctccag ctacaagtag
      661 tgtgtaggat ggtaatttgt cataggggag atttggttta agatggagtg tcatataaat
      721 accaccttgt ggtgagtaga aggaacgttg aaaacgtcct cggcctgctg tttgatagga
      781 agctagatag agggtatttg cttcatggcc taaatcaatt gcttcttttg catctagttg
      841 tgttgatttt gtttcgggtt taaagctgac tttaattgga agattttctt ctagaatctc
      901 tggaagaata aggtcaccat tcattagttt atatcctcta tttttgatac tatcaatttc
      961 aatgccttct tgttctagtc gcttgatagc tttccaaatt gatgttcggc ttagggatag
     1021 tttttctgcg attttttctc cgctgatata gtcggtttct ttagatagga tttggtagac
     1081 agcttggtag gatttcataa tgttgccttt ctcactagtt ggtattgaga gtattctttt
     1141 cttgtatgac ttggagtctg attaaagtat tgtttataag ctttcgaaaa atggagtgga
     1201 tctgaaaaac ctaccgagta tgcaattacc ttgatggact cttgggtatt ttcgagaagt
     1261 tgtctagctc ggtgcattcg aacgtagagt aggtattctt tgggtgataa ggtattaaat
     1321 tctttgaata cgcttgataa gtagcttctg tgaacggata gttcttttgc taaatcttga
     1381 attgtaagtg attgaggata gtggctatca attaatcgtt tgcattcaag atagagttgg
     1441 tgggttgatg aaatattctt ttttttctga ttgggagcaa tagttcccag atgaaacatc
     1501 agttcatgaa gttgtcccat gatatggagt tgagctaatt cacttgattt tgtaatctga
     1561 gcgaagcgga caatgtctga gatgagtttt gcagtagtct gggtatgaca agtttcagat
     1621 tggatgagat aggattgatc agaaatttga gaaagagcaa aataatcagg ggatttccct
     1681 ccagtgattc ctaaccagta gtaggcccaa ggttctttac tatctgcttg ataaaaggtt
     1741 agttcctctg gttttaatag aaagaaatct ccttctttta aatcaacaat tttatccttg
     1801 taatgaaatt ttccttgtcc tttagtaatg taatgtagga cgtatgtatc acgaatggct
     1861 ggaccaaaag agtaattagg tgtgcattcc tcatatccat aaaagcttag ggcaaggtcg
     1921 attgttccag tctggtattc tgaaaaaact aacatgtgct acctcctacc taacatttta
     1981 ccatattctt gagacatttt tctattttgg aaagcgattt caggtgataa aatataatca
     2041 ataaagtgat gaggtgaagt aaatgggagt taggatagag aataatctat tttatgttga
     2101 gagtaaaaat ctaagtttga ttattgaaaa tcgaaatggc tacttacttt tgaaacattt
     2161 aggaaagact attaagaact ataaaggttc caatagtgtt tatgaacgag accatgcctt
     2221 ttcaggaaat ccaacggcta ctaatcgaac ctttagttta gatactcagc gacagatttt
     2281 tggacaacat ggcttaggag attttaggaa accaactata caggttcagc atagtgtaac
     2341 tgaagtaaca gactttcgat ttgtagaagc aaagatttta aaaggtcaga atggtccaca
     2401 gggcttacct tctccacata gcatggacga tacagagact cttgtcttaa tgttagaaga
     2461 ttctaaggct caacttagtc tgactttgta ttatactact tttaataatg atgcgactat
     2521 tactagctac agtaaattag ataataatag taatcaggaa gttgtcatcc ataaagattt
     2581 ttcttttatg gctgattttc cagctgcaga ttatgaaata gcaactctgc agggtgctta
     2641 tgctcgtgaa aagactgtta gacgtcaaca ggtagaacaa ggaatctttt cgattagttc
     2701 aaaccgaggt gcttctggtc atgctcaaac accagctctt ctactatgcg aacaaggagt
     2761 cacagaggat gctgggaatg tgtttgctat tcaactaatg tatagtggca actttgaagc
     2821 ttttgttcaa aaaaatcaat tgaatgaagt tcgggtggct attggcatta atccagaaaa
     2881 cttttcttgg aagttagccc ctgaggaata ctttgaaaca ccggtagctt tagtgactca
     2941 ttcagatcag ggattaactg gtattagtca tgaaagtcag aattttgtac tgaagcacat
     3001 tatgctaagt gaattttcta aaaaagaacg tccaattcta atcaataact gggaagctac
     3061 ttactttgac tttcagagag aaaaactgtt agagttagca gatgaagcta agaaagttgg
     3121 cattgaactt tttgtattag atgatggttg gtttggcaat cgttttgatg ataatcgtgc
     3181 tttaggtgat tgggttgtta atgaggaaaa actgggtgga agtctagaaa gtctgatttc
     3241 agctatccat gaaagaggtt tgcagtttgg actttggtta gaacccgaaa tgatttctgt
     3301 agatagtgat ttgtatcgtc aacatcctga ctgggctatt caggttcctg attatgagca
     3361 tacttattct cggaatcaat tagtacttaa tcttgccaat cctcaggtag tagaatactt
     3421 gaaaagtgtc ttagatcaac tcctatctta tcatgagatt gattacatta aatgggatat
     3481 gaaccgcaat atcactaagc tagggaatgg attaacttat ctagagacac agatgcaatc
     3541 tcatcagtac atgctggggc tttacgaact cgtttcttat ctgacagaga agcacagcca
     3601 tattctcttt gagtcctgct ctggtggtgg tggacgaaat gatcttggta tgatacgcta
     3661 tttcccacaa gtctgggcta gtgataatac tgatgccatt gcacgtttac caattcaata
     3721 cggttcatcc tatctctatc caaccatttc tatgggggct catgtgtcag cagtaccgaa
     3781 tcatcagatg ggacgaatga caccattaga aacacgtggc catgtagcaa tgatgggaaa
     3841 tttgggctat gagcttgatt tgacaaattt atcagatgaa gagaaagcta cgattgctaa
     3901 tcaggtgaac ttgtataaag aattacgacc agtagttcag ttaggacagc agtatagact
     3961 aattaatcct gatactgcat ccaatgaagc agctgtacaa tttaattacg gaaatcaaac
     4021 gattgtaacc tacgttcgcg ttttatctgt tgtagagacc atggaaacaa ctttaaaatt
     4081 aaaagatttg gatgaagagg gactatataa attacaggaa aatggcgaag tttactcagg
     4141 tgcagaacta atgtatgcgg gcttaactgt tatcttatcc caaggagatt ttttgagtag
     4201 acagtatatt tttagaaaac tatgatatgt actgacccca aaagttagat ttattctgtc
     4261 taacttttgg ggtgcagttc aataagggtg tataaattta taaaggaggc tttctaatgg
     4321 aatggtataa aaaaatcgga cttcttgcaa ctacaggttt agctttgttt gggctcggcg
     4381 cttgctccaa ctatggtaaa tctgcggatg gcacagtgac catcgagtat ttcaaccaga
     4441 aaaaagaaat gaccaaaacc ttggaagaaa tcactcgtga ttttgagaag gaaaacccta
     4501 agatcaaggt caaagtcgtc aatgtaccaa atgctggtga agtattgaag acacgcgttc
     4561 tcgcaggaga tgtgcctgat gtggtcaata tttacccaca gtccatcgaa ctgcaagaat
     4621 gggcaaaagc aggtgttttt gaagatttga gcaacaaaga ctacctgaaa cgcgtgaaaa
     4681 atgcctacgc tgaaaaatat gctgtaaacg aaaaagttta taacgttcct tttacagcta
     4741 atgcttatgg aatttactac aacaaagata aattcgaaga actgggcttg aaggttcctg
     4801 aaacctggga tgaatttgaa cagttagtca aagatatcgt tgctaaagga caaacatcat
     4861 ttggaattgc aggtgcagat gcttggacac tcaatggtta caatcaatta gcctttgcga
     4921 cagcaacagg tggaggaaaa gaagcaaatc aataccttcg ttattctcaa ccaaatgcca
     4981 ttaaattgtc ggatccgatt atgaaagatg atatcaaggt catggacatc cttcgcatca
     5041 atggctctaa gcaaaagaac tgggaaggtg ctggctatac cgatgttatc ggagccttcg
     5101 cacgtgggga tgtcctcatg acaccaaatg ggtcttgggc gatcacagcg attaatgaac
     5161 aaaaaccgaa ctttaagatt ggaaccttca tgattccagg aaaagaaaaa ggacaaagct
     5221 taaccgttgg tgcgggagac ttggcatggt ctatctcagc caccaccaaa catccaaaag
     5281 aagccaatgc ctttgtggaa tatatgaccc gtccagaagt catgcaaaaa tactacgatg
     5341 tggacggatc tccaacagcg atcgaagggg tcaaacaagc aggagaagat tcaccgcttg
     5401 ctggtatgac cgaatatgcc tttacggatc gtcacttggt ctggttgcaa caatactgga
     5461 ccagtgaagc agacttccat accttgacca tgaactatgt cttgaccggt gataaacaag
     5521 gcatggtcaa tgatttgaat gccttcttta acccgatgaa agcggatgtg gattaggagt
     5581 agagagacta tgaaaaaagt attacaaaaa tattgggcat gggcttttgt ggtcatcccc
     5641 ctcttgttac aagcaatttt cttctatgtg ccgatgtttc aaggagcctt ttacagtttt
     5701 accaactgga caggattgac ttacaactac aaatttgtcg gcttaaacaa ctttaagctc
     5761 ctcttcatgg atccaaaatt catgaatgcg attggcttta ccgcaatcat tacgattgcc
     5821 atggtggttg gtgagattgc actcgggatc ttcattgcgc gtgtcttgaa ctctaaaatc
     5881 aaaggccaaa ccttcttccg tgcttggttc ttcttcccag ctgttttatc tggtttgaca
     5941 gtggctttga tcttcaagca agtcttcaac tacggtcttc cagcgattgg gaatgccctt
     6001 catattgaat ttctccaaac cagtctttta gggactaagt ggggagcaat ctttgcggct
     6061 gtctttgtcc ttctttggca aggggtggct atgcccatca tcatcttcct agctggtttg
     6121 caatctattc caactgagat tacagaggca gcaaggattg atggtgcgac tagcaagcaa
     6181 gttttctgga acattgaatt gccttacttg ctaccaagtg tctctatggt ctttatccta
     6241 gccctaaaag gtgggctgac tgcctttgac caagtctttg ccatgaccgg tggtggtcca
     6301 aacaatgcca caacctcact tgggctcttg gtttataact atgcctttaa aaacaaccaa
     6361 ttcggttatg ccaatgccat tgccgtaatc ttgttcctct taattgtagt gatttcgatc
     6421 atccaattga gagtatctaa gaaatttgaa atttaagagg agaagcatga tgaaacaaga
     6481 tgaaagaaaa gccctgattg gcaaatacat tctattgatt ctaggatcgg ttctgatttt
     6541 agtgccgctc cttgctaccc tctttagttc cttcaaaccc actaaggata ttgtagataa
     6601 tttctttggc tttccaacca acttcacatg ggacaacttt agccgtctct tagctgatgg
     6661 gattggaggc tattattgga actctgtcgt catcactgtc ttgtctttac ttgcagtaat
     6721 gatctttatc cctatggcag cctactccat cgctcgcaat atgagtaaaa gaaaagtctt
     6781 taccatcatg tataccctct taatcctcgg aatcttcgta cctttccaag tcatcatgat
     6841 tccgattacg gttatgatga gtaaactcgg tttggctaat acctttggtt tgatcttgct
     6901 ctacttgacc tatgcgattc cacagaccct ctttctctat gttggctata tcaaaatctc
     6961 gattccagaa agtctggatg aagcagcaga gatcgatggg gctaatcaat ttacaaccta
     7021 tttccgcatc atcttcccaa tgatgaaacc gatgcatgcg acaaccatga tcatcaatgc
     7081 cctttggttc tggaatgact tcatgttgcc actccttgtc ttgaaccggg attccaaaat
     7141 gtggactctg cctttgttcc aatacaacta cgcaggccaa tatttcaacg actacggacc
     7201 aagctttgcc tcttacgtgg tcggcattat cagtatcacc attgtctatc tcttcttcca
     7261 acgccatatc atttcaggaa tgagcaacgg ggcagtgaag taatactcaa tgaaaatcaa
     7321 agagcaaact aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtggatga
     7381 aactgacgaa gtcagtaacc atacctacgg taaggtgaag ctgacgtggt ttgaagagat
     7441 tttcgaagag tataagaaga gctcatggaa tctatagtct ttgacacaga ggaagagttc
     7501 agcttgttga taattctatg tagaaaacaa gaaagtagta ctttgtttat cacagtctat
     7561 attagtacga gtttgatgat aaatagagat ttaaaatgaa tttgatttac gaagccagat
     7621 catatgagac ttggcttcaa ttagaaaaag gagagtaatg atgccgattc aaaataaaac
     7681 catgttgatt acctattctg atagccttgg aaataatctt aaagacttat atgataattt
     7741 ggaagagcat tttggagatg ctattggagg agttcacctt ttaccatttt tcccatcaac
     7801 aggtgatcgt ggatttgcgc cagttgacta cgacgaagtg gactcagctt ttggtgattg
     7861 ggaggatgtt aagcgtttag gtgagagata ttatcttatg tttgatttta tgattaatca
     7921 tatttctcgt caatccaagt attataagga ctatcaagaa aaacatgaag ccagtgaatt
     7981 taaagctctc tttttaaact gggataagtt ttggccagaa aaccgtccga cacagtctga
     8041 tgtagattta atttacaagc gtaaggatcg tgcaccaaag caagagattg tgtttgaaga
     8101 tggttcagtg gaacatttgt ggaatacctt tggtgaggag cagattgatc ttgatgtgac
     8161 caaagaagta actatggaat ttatccgtaa gaccattcag cacttggcaa gtaatgggtg
     8221 tgatttgatt cgtctagatg cctttgctta tgcagtgaag aaattggata ctaatgattt
     8281 ctttgtggaa ccagatattt gggatttatt ggacaaagtt cgagatatcg ctgctgagta
     8341 tgggacagag cttttacctg agattcatga acactattcg attcagttta aaatagcaga
     8401 ccatgattac tatgtttatg attttgctct tccaatggtg acactttata ctctttacag
     8461 ttccagaaca gagcgtttgg ctaagtggtt aaagatgagc ccgatgaagc aatttacgac
     8521 gctagatacc catgatggga ttggagtagt agatgtcaag gatatcctga ccgatgagga
     8581 gattgactat gcttcaaatg aactctataa ggttggagcc aatgtcaaac gtaagtactc
     8641 tagtgccgag tataacaact tagatatcta ccaaatcaat tcaacctact attcagcgct
     8701 tggagatgat gatgtcaagt attttctcgc tcgtctaatt caagcttttg ccccaggtat
     8761 tcctcaggtt tactatgtgg gtctattagc aggcaagaat gacttgaaat tattagaaga
     8821 aactaaagaa ggtcgaaata ttaatcgtca ttactatagc aacgaggaaa tagcaaaaga
     8881 agtgcaacga cctgttgtga aggcccttct caatctattt tctttccgta accgttcaga
     8941 agcctttgat ctagagggga ctactgagat agagacacca acagctcaca gcattgtaat
     9001 caaacgtcaa aataaagata agtccgtaac agcagtagta gaaattgatt tgcaaaatca
     9061 gacttatcgg gtaattgaga atggagttga agtaacattt tgaagccttg atatggtgga
     9121 taaaataggg tttttatttt ggaaaacgtt tcctttgttt tcaaattgct aaaaaagtgg
     9181 tacaatatag gatagcttac tattatctga atcagcagat ttggagagaa aggattcatt
     9241 ttgaaatcaa taggctttat tgaaaagctg aaggggttgt ctagtaaaga gctgatttta
     9301 ttgggaatta tcctaagtat ctttttaccc ttttatcttt ttgtagttgt actctgttta
     9361 tatattatca gtttgatttt tacaggagac atgaaaagta ttcttcagaa aatgggggag
     9421 catccgatgc tgcttctttt tcttagctat agtactgtta tatccattct tgcacaaaat
     9481 tggatgggtc ttgtggcttc agtaggaatg tttctattta ctattttctt tttgcactat
     9541 cagtcgattt tatcccataa attctttcga ttgattttgc agttcgtctt gtttggtagt
     9601 gtcttgtcag ctgcttttgc cagtttagaa catttccaaa ttgtgaagaa atttaactat
     9661 gcttttcttt cacccaatat gcaggtgtgg catcagaacc gggcagaagt gaccttcttt
     9721 aatcctaatt attatggaat tatttgttgt ttctgtatta tgattgcttt ctatctgttt
     9781 acaacgacca agttgaattg gttgaaagta ttctgtgtga ttgcaggctt tgttaatctc
     9841 tttggtttga actttactca aaatcgaact gcctttcctg ctattatcgc tggagcaatt
     9901 atctatctct ttacgactat taaaaactgg aaggcctttt ggcttagtat tggggtcttc
     9961 gcgattggtt tgagtttcct cttttctagt gatttgggag ttcgaatggg tactttagac
    10021 tcttctatgg aagaacgcat ttctatctgg gatgctggga tggccttgtt taagcaaaat
    10081 cctttttggg gtgaagggcc attgacctat atgcactctt atcctcggat acatgctcct
    10141 tatcatgaac atgcccacag tctttatatt gatacgattc tgagttacgg aattgtgggt
    10201 accattttat tagttttgtc ttctgttgct cctgttcgct tgatgatgga tatgagtcag
    10261 gagtcgggga aacgtccgat tatcggcctt tatctatctt tccttacagt ggttgctgtg
    10321 cacggaattt ttgacttggc tctcttctgg attcagtcag gctttatttt cttgctagtt
    10381 atgtgcagta ttccattgga gcatcgaatg ttggtatcgg acatgacgga ttaagtttta
    10441 taagtttgaa atcttctacc ttgggtagga gattttttta tacgatgggc atgtcgtgca
    10501 tctgaggtgt aagtcctctg tagggatcca ctaccggtga atacaatagc gctccccaag
    10561 cctgactatc gtgaggtagc ggattaaaat ggtctgggga tagatcgttt taagtctgac
    10621 gcaggaaata agaattgtca gagaaagcaa tagtgaaatc gtaggctatg aatagaaacg
    10681 tgatagcgag gagcatatag tggataaggt agcgagaaat tttaaagtat aaaagatagt
    10741 cttaatctat atatgtaaat cacaaaagta attgaattcg gaataaggtt ggtgtgaaaa
    10801 gacatagtaa actgaatctt gaatactaga atgatacttc tacaatattg ttataattta
    10861 atttgaattc caaaatcaca ttgttccgtc cgtatttcat tttactgtat gtcttcctaa
    10921 actccaaagt ttctgcgtca aatttcctat tttcactgta gtctttgaca cttcgaacga
    10981 caatttgaat catatattca aaaaaaaatg aaaaatttgt gccaacaagc ctaaatattc
    11041 tgaaaatttg tttgattttt aatggataat gtgatataat ggttgtcaat ctataattca
    11101 cataaacaga ggagtagaaa atgaaaaaaa atcgtgtatt tgctacagca ggtcttgttt
    11161 tattagcagc aggtgtactt gcagcatgca gttcttcaaa atcatctgat tcatcagccc
    11221 ctaaagctta tggctatgtt tatacagcag acccagaaac cttggactac ctgatttcaa
    11281 gtaaaaatag tacaacagta gtgacttcaa atgggattga tggtttattc actaacgata
    11341 attacggtaa tcttgctcct gcagttgcag aggattggga agtctctaag gatggtttga
    11401 cctacactta taagattcgt aaaggggtta aatggtttac ctctgatgga gaagaatatg
    11461 cagaggtgac ggctaaagat ttcgtgaacg gtttaaaaca cgcagcagat aaaaaatcag
    11521 aagctatgta tttagctgaa aattcggtta aaggcttggc agattatcta tcaggaactt
    11581 caacagattt ttcaacagtt ggtgtcaagg cggttgatga ttatacgtta caatacactt
    11641 tgaaccagcc tgaaccgttc tggaactcta agttgaccta ttctattttc tggcctctga
    11701 atgaagaatt cgaaacatca aaaggaagcg attttgctaa accaacagat ccgacatcct
    11761 tgctttataa tggtccattc ttgttgaaag ggttgactgc aaaatcttct gtagagtttg
    11821 taaaaaatga gcaatattgg gataaagaaa atgtccacct agatactatc aatctagctt
    11881 actatgatgg atcagatcag gagtcgctag agcgtaactt cactagtgga gcttatagtt
    11941 atgcccgtct ttaccctacc agctccaact attctaaggt tgcagaagaa tacaaggaca
    12001 atatctatta cacacaatca ggctctggga ttgctggtct gggtgtgaat attgatcgcc
    12061 aaagttacaa ctatacttct aaaactacag attcagagaa agtagctact aagaaggcat
    12121 tgcttaacaa agatttccgt caagccttga attttgctct tgatcgctca gcttactcag
    12181 ctcaaatcaa tggtaaagat ggagcagctt tagcagttcg taatttattt gtaaaaccag
    12241 actttgtttc agctggtgag aagacctttg gtgatttagt cgctgctcaa cttcctgctt
    12301 atggtgatga gtggaaaggt gtgaatttag ctgatgggca ggatggttta ttcaatgctg
    12361 acaaggccaa ggcagagttt gcgaaagcta agaaagcttt agaagcagac ggcgttcagt
    12421 ttcctattca tctggacgtt ccagtagacc aagcatcaaa aaactacata tctcgtattc
    12481 agtcctttaa acaatctgta gaaacagttc ttggtgttga aaatgtcgtt gttgatattc
    12541 aacaaatgac aagtgatgaa ttccttaata ttacttacta tgctgccaat gcttcatctg
    12601 aggattggga tgtatcagga ggagtttcat gggggccaga ctatcaagac ccatctactt
    12661 acctggatat tttaaaaaca actagcagtg aaactacaaa aacatattta ggatttgata
    12721 atccaaatag cccttcagta gttcaagttg gtttgaaaga atacgataaa ttagttgatg
    12781 aagctgccaa agagacaagc gacttgaatg tccgttatga aaaatatgca gcggctcaag
    12841 catggttgac agatagttca ctctttattc ctgctatggc ttcttctggt gcagcaccag
    12901 tgctttcacg aattgttcca tttactggag cttctgcgca aacaggctct aaggggtcag
    12961 atgtttactt caaatatttg aaattacaag ataaagcggt gactaaggaa gagtatgaaa
    13021 aagctcgtga aaaatggttg aaagaaaaag ctgaatcaaa tgagaaagct caaaaagaat
    13081 tggcaagtca tgtgaagtaa ataatttcta atagaacttt ctctccatga ggagaaggtt
    13141 cttttgggat ttttaaagga aataatatga aaaaatatat ttttatgcgt gttttgcggt
    13201 cattggtttc gattttctta gtaacgactt tgacctacac gattatctat accttggttc
    13261 ctcgaaaatt gattttcaag caggatccta actataataa aattgcgaca acggctgata
    13321 aacgtgataa ctatgaaaat actgtgtttg agcgtatggg ctacattgag tattacgata
    13381 ctaaagagtt gcaagaaaag gcaagtagca tggattcttc tgtaacagta gaagcaaatg
    13441 cgaccaataa agctatttat gaaaagtaca tcaatcaatt aggtcatggt tggactttgg
    13501 gagaatttac tgaaagtggt caattctatg ctactcgtga aattccaatt tttgaacgtg
    13561 tttttcactt ctatgctaac ttgattgaca ttgaccatac aaataaaatc caagaccctg
    13621 aaaatccaga cttgaaacgc taccttcgtt ttgaaaatga tccagctatc ggatggtcat
    13681 tggtcggttc aggaactaaa cataaatatc tcttgtactt taacagtcag ttcccatttg
    13741 ttcatcaaaa ctttgtgaac ttgaatttag gtgactctta cccaacctat gctaatacac
    13801 cagttcttca ggttattact caaggtcaag gacaaaccaa aactgcccaa gttcagttcc
    13861 caacaggtaa gaaaacgtct tctgtaaata tttactcaag aacctacaag tcacctagtc
    13921 aggctgactc tcgtgaagta gctagctatg ggaaagatga tccttataca gcgactgaaa
    13981 gtaattacca atatccatct atgattgtca gctctgctat tactggtttg attggtttgg
    14041 ttcttgccta tgctcttgcc gtgccacttg gttcagccat ggctcgtttc aagaacactt
    14101 ggattgatag cctctcaaca gggactttga ccttcttgct tgctcttcca acgattgcct
    14161 tggtttacat cgttcgattg attggatcat ctattgccct tccagattca ttccctatct
    14221 tgggagctgg agattggcgt tcttacgttt taccagcagt catccttggt ttgttgggtg
    14281 ctcctgttac agccatttgg attcgtcgtt acatgattga cttgcaatct caagactttg
    14341 ttcgtttcgc tcgtgcaaaa ggtttgtctg aaaaagaaat ttcaaacaaa cacatcttta
    14401 aaaatgccat ggttccgctg gtttcaggaa ttcctggtgc cattattggg gttatcggtg
    14461 gtgcaaccct tactgaaaca gtcttcgcct tcccaggtat gggtaaaatg ttgattgact
    14521 ctgtaaaagc atctaataac tctatggtcg ttggtcttgt cttcatcttt acatgtattt
    14581 ctatcttctc acgtcttttg ggagatattt ggatgactat tattgaccca cgtattaaat
    14641 tgactgagaa aggaggcaaa taatgtctac aatcgataaa gaaaaatttc agtttgtaaa
    14701 acgtgacgat tttgcctctg aaactattga tgcgccagca tattcttact ggaaatcagt
    14761 gtttaaacaa tttatgaaga aaaaatcaac tgtagtcatg ttgggaatct tggtagccat
    14821 cattttgata agtttcatct acccaatgtt ttctaagttt gatttcaatg atgtcagcaa
    14881 ggtaaacgac tttagtgttc gttatatcaa gccaaatgcg gagcattggt tcggtactga
    14941 cagtaacggt aaatcgctct ttgacggtgt ctggttcgga gctcgtaact ccatcctcat
    15001 ttctgtgatt gcgacagtga ttaacttggt tatcggtgtt tttgtcggtg gtatttgggg
    15061 tatttcaaaa tcagttgacc gtgtcatgat ggaagtttac aacgtcatct caaacatccc
    15121 acctcttttg attgttattg tcttgactta ctcaatcgga gctggattct ggaatctgat
    15181 ttttgccatg agcgtaacaa catggattgg tattgccttc atgatccgtg tgcaaatctt
    15241 gcgctatcgt gacttggaat acaacttggc gtcacgtact ttgggaacac caaccttgaa
    15301 gattgttgcc aaaaatatca tgcctcaatt ggtatctgtt attgtgacaa ccatgactca
    15361 aatgcttcca agctttatct catacgaagc cttcttgtct ttcttcggtc ttggattacc
    15421 gattacagtg ccaagtttgg gtcgtttgat ttcggattat tcacaaaacg taacaaccaa
    15481 tgcttacttg ttctggattc cattgacaac ccttgtcttg gtatccttgt cccttttcgt
    15541 agttggtcaa aacttagcgg atgctagtga tccacgtaca catagatagg agtagaaatg
    15601 acaaaagaaa aaaatgtaat tttgactgct cgcgatattg tcgtggaatt tgacgttcgt
    15661 gacaaagtat tgacagccat tcgcggcgtt tcccttgaac tagtcgaagg agaagtatta
    15721 gccttggtag gtgagtcagg atcaggtaaa tctgttttga caaagacctt cacaggtatg
    15781 ctcgaagaaa atggtcgcat tgcccaaggt agtattgact accgtggtca ggacttgaca
    15841 gctttatctt ctcacaagga ttgggaacaa attcgtggtg ctaagattgc gactatcttc
    15901 caggacccaa tgactagttt ggaccccatt aaaacaattg gtagtcagat tacagaagtt
    15961 attgtaaaac accaaggaaa aacagctaaa gaagcgaaag aattggccat tgactacatg
    16021 aataaggttg gcattccaga cgcagataga cgttttaatg aatacccatt ccaatattct
    16081 ggaggaatgc gtcaacgtat cgttattgcg attgcccttg cctgccgacc tgatgtcttg
    16141 atctgtgatg agccaacaac tgccttggat gtaactattc aagctcagat tattgatttg
    16201 ctaaaatctt tacaaaacga gtatcatttc acaacaatct ttattaccca cgaccttggt
    16261 gtggtggcaa gtattgcgga taaggtagcg gttatgtatg caggagaaat cgttgagtat
    16321 ggaacggttg aggaagtctt ctatgaccct cgccatccat atacatggag tctcttatct
    16381 agcttgcctc agcttgctga tgataaaggg gaactttact caatcccagg aacacctccg
    16441 tcactttata ctgacctgaa aggggatgct tttgctttgc gttctgacta cgcaatgcag
    16501 attgacttcg aacaaaaagc tcctcaattc tcagtatcaa agacacattg ggctaaaact
    16561 tggcttcttc atgaggatgc tccaaaagta gaaaaaccag ctgtgattgc aaatctccat
    16621 gataagatcc gtgaaaaaat gggatttgcc catctggctg actaggagga aggaaatgtc
    16681 tgaaaaatta gtagaaatca aagatttaga aatttccttc ggtgaaggaa gtaagaagtt
    16741 tgtcgcggtt aaaaatgcta acttctttat caacaaggga gaaactttct cgcttgtagg
    16801 tgagtccggt agtgggaaaa caactattgg tcgtgctatc atcggtctaa atgatacaag
    16861 taatggagat atcatttttg atggtcaaaa gattaatggt aagaaatcgc gtgaacaagc
    16921 tgcggaattg attcgtcgaa tccagatgat tttccaagac cctgccgcaa gtttgaatga
    16981 acgtgcgact gttgattata ttatttctga aggtctttac aatcaccgtt tatttaagga
    17041 tgaagaagaa cgtaaagaga aagttcaaaa tattatccgt gaagtaggtc ttcttgctga
    17101 gcacttgact cgttaccctc atgaattctc aggcggtcaa cgtcaacgta tcggtattgc
    17161 ccgtgccttg gtcatgcaac cagactttgt tattgcagat gagccaattt cagccttgga
    17221 cgtttctgta cgtgcccaag tcttgaactt gctcaaaaaa ttccaaaaag agctcggcct
    17281 gacctatctc ttcatcgccc atgacttgtc ggttgttcgc tttatttcag atcgtatcgc
    17341 agttatttac aagggtgtta ttgtagaggt tgcagaaaca gaagaattgt ttaacaatcc
    17401 aattcaccca tatactcaag ccttgctttc agcggtacca atcccagatc caatcttgga
    17461 acgtaagaag gtcttgaagg tttacgaccc aagtcaacac gactatgaga ctgataagcc
    17521 atctatggta gaaatccgtc caggtcacta tgtttgggcg aaccaagccg aattggcacg
    17581 ttatcaaaaa ggactaaact agtaatggtt ttataatttc catgtcaatt tttatggaaa
    17641 ttataaaact ttttttgttg gactgatttg aaaaactttg aaagcttcct gaaa
//
LOCUS       DAVMMR010000043        17422 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMMR010000043 DAVMMR010000000
VERSION     DAVMMR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.043"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(85..2517)
                     /locus_tag="VYS74_001440"
     CDS             complement(85..2517)
                     /locus_tag="VYS74_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3789464.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVAFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            complement(2519..2977)
                     /locus_tag="VYS74_001441"
     CDS             complement(2519..2977)
                     /locus_tag="VYS74_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV3789465.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(3096..3824)
                     /locus_tag="VYS74_001442"
     CDS             complement(3096..3824)
                     /locus_tag="VYS74_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789466.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(3824..4831)
                     /locus_tag="VYS74_001443"
     CDS             complement(3824..4831)
                     /locus_tag="VYS74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3789467.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(4869..5627)
                     /locus_tag="VYS74_001444"
     CDS             complement(4869..5627)
                     /locus_tag="VYS74_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789468.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(5590..5880)
                     /locus_tag="VYS74_001445"
     CDS             complement(5590..5880)
                     /locus_tag="VYS74_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV3789469.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     regulatory      complement(5951..6053)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6136..7482)
                     /gene="cbpD"
                     /locus_tag="VYS74_001446"
     CDS             complement(6136..7482)
                     /gene="cbpD"
                     /locus_tag="VYS74_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV3789470.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(7571..7837)
                     /locus_tag="VYS74_001447"
     CDS             complement(7571..7837)
                     /locus_tag="VYS74_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789471.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(7839..9191)
                     /gene="dnaB"
                     /locus_tag="VYS74_001448"
     CDS             complement(7839..9191)
                     /gene="dnaB"
                     /locus_tag="VYS74_001448"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV3789472.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(9235..9687)
                     /gene="rplI"
                     /locus_tag="VYS74_001449"
     CDS             complement(9235..9687)
                     /gene="rplI"
                     /locus_tag="VYS74_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV3789473.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(9684..11657)
                     /locus_tag="VYS74_001450"
     CDS             complement(9684..11657)
                     /locus_tag="VYS74_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV3789474.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(11793..12341)
                     /gene="raiA"
                     /locus_tag="VYS74_001451"
     CDS             complement(11793..12341)
                     /gene="raiA"
                     /locus_tag="VYS74_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV3789475.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(12421..13083)
                     /locus_tag="VYS74_001452"
     CDS             complement(12421..13083)
                     /locus_tag="VYS74_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV3789476.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(13080..14378)
                     /locus_tag="VYS74_001453"
     CDS             complement(13080..14378)
                     /locus_tag="VYS74_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3789477.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            14434..15069
                     /locus_tag="VYS74_001454"
     CDS             14434..15069
                     /locus_tag="VYS74_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV3789478.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            15354..16274
                     /gene="cysK"
                     /locus_tag="VYS74_001455"
     CDS             15354..16274
                     /gene="cysK"
                     /locus_tag="VYS74_001455"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV3789479.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(16471..16869)
                     /locus_tag="VYS74_001456"
     CDS             complement(16471..16869)
                     /locus_tag="VYS74_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789480.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            16860..17168
                     /locus_tag="VYS74_001457"
                     /pseudo
     CDS             16860..17168
                     /locus_tag="VYS74_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5154 a         3978 c         2994 g         5296 t
ORIGIN      
        1 agctagctgc ctagtttgct ctttgatttt cattgagttt cactccactg tgacttatgg
       61 tcagatgctg atacttttat tcttttatgc aatatcaaat tttaactggc ctgctttgac
      121 accaatctta agtgtgttgc ctgccactaa atctccctta agaagaagtt ctgccaactt
      181 gtcctccact tctgtttgca gggttctgcg aagtgggcga gctcccatct ctgggtcata
      241 tccttgattt gctaacaatt tcagagctga agcttgtaat ttcaagtcaa tgcctttttc
      301 agccaaactt gccactaaag gcttgaccat aatcttcacc acttcctgca tatggtcact
      361 agataggcta tggaaggcca ccttctcatc aatgcggttg atgaattccg gtctataagc
      421 ttttttcagt tcttcaaaca tgcgtttttc catattttcc tggtcaaaac gaatatcctt
      481 agccccaaaa ccaacagtct tatcatcacg aagggcagtc gcacctagat tcgatgtcat
      541 aatgataatg gtatttgaaa aatcgacctt gcgtcccttg ctatctgtca agacaccgtc
      601 atccagaacc tgcaagagaa cattaaagat atctgggtgg gccttctcta cctcatcaaa
      661 gaggagaacg gaatagggtt tattgcgaac cttctctgtc agctccccac cttcttcgta
      721 acccacatag cctggaggag ctccatttag acggctggct gcgaatttct ccatatactc
      781 actcatatca aagcggataa gggctgattc gtcatcaaag agaacttctg ccaaagcctt
      841 ggccaactcg gtcttaccga cacctgtggg tcctaggaac ataaaggaac cgatcggacg
      901 cttgtgactg cgaattcctg actgattacg gcgaatggca cgactaatac ttgaaacagc
      961 ttgatcttga ccgataaccc gtttatggag ttctgcttca agatttagat atttcttagc
     1021 gtcagtctga gtcagttttt gaactgggat tcctgacaag cgactcaagg tggtcaaaat
     1081 atcagactct gtcaccaagt ctttgtagac aggtacttcc tcttcttttg cgattagctg
     1141 ggctgcctgt ttccacttgc catccatcag ggccttgtca gctggactca aatctgaatc
     1201 gtctgctttt acatgctttg ccttattttg cactgttgct gctgcttcgt ccaagaggtc
     1261 gatagcagag tctggcaagt gacgactggt taaataacga tgagccatct taaccgctgt
     1321 ttcaaccgct tcatctgtga tttgtacacg gtgatgtttc tcataagtcg ccttcaaacc
     1381 ttgtaaaata gtcatactat ctgccacact tggttcttca atcgtcactt tagcgaaacg
     1441 acgagaaaga gccgcatctt tttcgatatg tttttgatat tcttcctgag tggtggcacc
     1501 aaccgttctc aaagttccac gcgccaaggc tggtttcaag atattggccg catccagagt
     1561 cgaatcaatc ccgctaccag aacccatgat ggtgtggagt tcatcgataa agaggatgac
     1621 ttggccatct tcttcaatat ccttgatgat attattcatg cgttcttcaa agtcaccacg
     1681 gaagcgtgtc cctgcaacga cattcatcaa atcaagttct aacacgcgca tcttagccat
     1741 ttccgcaggc acgtcaccac tagcaatacg ctgggcaaga ccaagggcca gagctgtttt
     1801 cccgacacca gcatccccaa ccaagacagg gttgttctta gtcttacggc tcaatatttg
     1861 aatcatacgc gagatttcct tgtcccgacc gatgactggt tctaacttgc cagaacgcgc
     1921 ttgctctgtc aaatcatgcg tatagtcctc gagaccacca ctaggagtct gcggcatgcc
     1981 catcatattg gccatagaat tttgcttgtc agctactgta cgatggcgtt ggcgtaaagc
     2041 cttgagatct tcacgagtcc agcctgcacg ttcttctaaa tttcgacgaa gggcagcaat
     2101 cttgacctga tctttcttgt cttcataaga aaaaccagcc ctctccaaga tacgagtcgc
     2161 caaggcattg ccatcatgca aaatcgcata gaggacgtgc tctgtcccta gtaccttagc
     2221 atggaccact gacgctacat actctgcttc atcaaaaaga acctgcaaac gacgggagaa
     2281 cggcaattcc gtaaaggttt catcctggct atagtccgtt tcagtcagtt ccaaagccac
     2341 ctcttctaaa cggtccatct catacggata atcatttaaa gttgcccctg ctacactata
     2401 actgtgatta gacatggcaa tcaacaagtg ccacgactct agataacgag ctccaaaatg
     2461 tccagcaacc atgtaggcac tttcgataca ttcattcaat gcttttgaat agttcatctt
     2521 acttcccttt tctatctacc tcttgtatga cctgacgtag catgtttgct cgaacaactg
     2581 gagcttcttc tcctaaaacg cgatccaaag ctactgattc tagcaaattc atctcctgct
     2641 tggtcatcaa ttcctgctca accaaaagct ggagaatatc ctcataaatt tcttgactga
     2701 ctcgctcacc aatcgagtaa agcagatccc ggagcatttc atgatgacta gaaaactcaa
     2761 tccgtcctat acgaatgtag cctccgccac cacgcttact ttcaaccaag tagcctctac
     2821 tttccgtaaa gcgtgtcttg atcacgtagt taatctgact aggaacaacc tgaaaggtat
     2881 ctgccaactg actccgttgc aactccacga taccagattg atctaaaatc gccttgatgt
     2941 aggcctcaat atgatccgat gtatttttaa atctcataac aaacccacct ctctctttaa
     3001 accttgacct tgtttgacta ttataccgaa attttctcat ttttaaaaga aaaagggcgc
     3061 tggtaaagga taatcttcac caactcccta tttttctact tatctaagcc taattctgcc
     3121 aagatttgac gtttgtaggc aatcttttgg acttccttgt cctcatcttc agaccaatct
     3181 agtttaattt ctgaaacaat ctgcccaggg cgatttttca agatatagat gcggtcgctg
     3241 agattgaggg cctcctcaat actatgcgtg atgatcagag ttgttagctg caactgtttg
     3301 tgaatctcaa gataccaagc gtggagttcc atctttgtca tctcatccaa ggcgctaaag
     3361 gcctcatcta agagaaagag cttgtgccca aaaaggtagg tccggagtaa ggctacacgc
     3421 tggcgcatcc caccgctaag ttcatgagga tacttgtctc ttacagctgt cagctggaag
     3481 gtcgcaagaa ttttatccgc tcgggaaata gcttctgcct tatccacctt ttgaatcaag
     3541 aggggcagaa tgatatttcc aagcaccgtc ttgtgctcca agagcagatc cttttgcaac
     3601 atataactca cgcgcccctt gggattttct tcaccatcaa ggacaattct ccctgactga
     3661 acttctaaaa tcccagcgat tagattaaag agggtggtct ttccaacacc acttgggcct
     3721 aggatggaaa ccacttcgcc tgaagtcacc tgtaggttga tatcctctaa aatcctctcc
     3781 tgaccatagg cataactgac gtgctctagt ctaatttctg tcattatttc acaaattcgt
     3841 tggtgaagcc tttgtctgtc aagtcttctt taaggatacc attttcttta tcccatttat
     3901 agaaagcatt ccagcgagct gcgtcaaatt gaccccattt ttccttgtcg cttgcgtatt
     3961 cttttgacaa gtatttttga gattcgatga caaagtcacg tttttccttg agttcaggtg
     4021 cattcttgat gagaatatct gcagcttctt ctggatgttc catggcatat tggtagcctt
     4081 ttttgatggc ttggatgact ttgcgagctt cttctttgtt atctttcaga tagtcgttgt
     4141 ttgcgatgat aactggtgaa tagtagtcaa actccttgac atagtctttc aagtacatga
     4201 agttagcatc tacaccttga gatttagcaa ggataccatc ccaaccgtag taaatccaag
     4261 cagtatcaaa gacgccattg gcaatcggtg tgattgagtt tgagtcgtta tttggtactt
     4321 tttcaacctt ctcaaagtct ccaccttgag attctaccaa ggttttcaac atagcaagtt
     4381 cagttgggtc attccatgtc ccatatttct taccaaccaa gtcttttgga ctgcttacat
     4441 tatcagattt acgagagatg attcctgatg tattgtgttc aacaatagct gcaacggcag
     4501 tgattcctgc tcctttttcc aatttcttag ccatgtagtc ttggaaatac actgcaaatg
     4561 gtgcctttcc gttgataacc aagtcagaag aactttcttc tggtggcaat ttcaaatcaa
     4621 catccactcc agcttctttg aaataacctt tttccttggc aacataaagc cctgtgtggt
     4681 tggtatttgg tgtccagtct aggataaagt caaccttctt aagttctgcc tctttgttgt
     4741 ctttagaagc agttccttga ccacaggcca caagcacaac agctacaaga gctgttacaa
     4801 gcgttaaaaa cactttccat gttttcttca ttttgatttc ctcttttctt tttcaaaaac
     4861 gctctatttt aagtacgttt ccatttaatc acatattttt cactgatatc gaccagcttc
     4921 atacccaaaa gactgataat cgacaccaga ataataatgg caaacatggt atcatactga
     4981 aacagttttt tagactgaat catataaaca ccaagacctt caaaacctcc caaccactca
     5041 gataccactg ttgtgataaa agcgtaggag acactgaccc tcagaccagc ataaaagtag
     5101 ggcaggctga ctggaatttt aaaatgccac aggatttgcc aaggcttggc ccgcatcaga
     5161 ctaaacaagg tcagcatatc cttgtcgcaa tgcctaaaac cgtccaaaat gctgacgatg
     5221 atgggaaaag ttgtcgttaa gataatcaag acaatcttgg gcaaaatccc ataacctagc
     5281 cacaagacca ggataggagc tatggcaatg gtcggaatgg tctgaataac caccatcata
     5341 gggtaaatca ggtcattgag ccaagtcaaa ctatccatga gcacagccat aagacaggca
     5401 atcaaaactc ccaaaatcag ccccagtaaa gccactctca aggtcgccca gctatggtgc
     5461 cagagaaatt ctctgtcacg aacaaagggc tggagaattt caagaggtgt cggcaggata
     5521 aacttgggga gaagtttaag aaaacctgct aactgccaga ttgacaagac tccgagaaag
     5581 cccaatagac taatgtggcg tctcagtata cttctcaagt ttctcatcaa tacttaaaat
     5641 ctctcctaca tttattttga cattggcaaa gacattgtct gcctcctgcc ctgccacttc
     5701 cagcgcttct tttagaatgc gcataagctc atcaaactcc ccttccaaga ccgtttcaaa
     5761 tggtgtcact accatcgtca cttcttgagt ttgcagataa gcaatgacct gatcaataac
     5821 agctatccga tcaatcccct gtgctagggg taaaacttgc aaggcaatgc ttgctttcat
     5881 aagaacctcc tcttctcgtt ttccttttta aaaaaagaaa aacctttacc atacatggaa
     5941 aaggtgcaaa attagactaa gtatcgttcc ctccgctggc attacccaga tcaggtgcgg
     6001 tcgaagttta acacttcctc tcagactgta cacagactcc catatattat atgtatatat
     6061 gatagcgcaa aataagaaaa atgtcaagga aactgcttac agaaaagagc aggaaatttc
     6121 tcctactcca attttctata ctcgttctcc atcactgttt actctgtagc catccaccgt
     6181 cgtattaaca gctaaggcgc ctgaagagta agaataatac cacttaccag agacctggta
     6241 ccagcctgtt ttcattgccc ctgaactacc cagataatac cacgagccat tctcctttaa
     6301 ccatcctgtc tgcatttcac cagataattt caaataatac cagctagaac catctttcag
     6361 ccatcctgtt gatttagaac catttgattt gaaatgatac caactaccat ttattttctt
     6421 ccaaccgaca gttgaactat tattggatcc atagctagag atatttcctg attgattctg
     6481 ataattttgt gaagaagtca ctccctctag ctgtatatag cgacgacttc cgttataagc
     6541 cgtataactc aaccacttgt agccgtcttt ttcaagtatc tgatcataat gaaccttctc
     6601 cccaggataa tagtaatcaa tcacagttgc actaactagg ggttcagttt tgatagcaga
     6661 cttggtctta aaataatgag ttccacctgt tgaagaaaga acagaattac ctagaggatt
     6721 tttattcaca gcctccaatt gaacataacg atagcttcca ttataggcag tataactcaa
     6781 ccacttgtag ccgtcttttt cgagaatttg atcataatga accttctctc caggataata
     6841 gtaatcaatc acagttgcac taactagggg ttcagtttta atagcagact tggtcttaaa
     6901 ataatgagtt ccgcctgttg aagttgagga ttgactattc ccaacactgc caccatccaa
     6961 atctttaaaa tgaataaatc ctgtcatcgt gtttgctttt ataattcgtt tattatagga
     7021 ttctgtataa ccatagttat attcctcaat ctcaatctga tctcccatta catttgacac
     7081 ccaggcaaca tgaccataag ttcctgcagt agaccaagta atggaaccaa tcgtcggtgt
     7141 attatctaca cgataacctt cccgacgagc acgatgtccc cattcattcg catttccata
     7201 agctgccgga atttcaaaac cattgacatt actcaaacga aaggctacaa aagaagtaca
     7261 ctgacgagaa tacatgcgcc actgatcaat ctcctggctc ccatttttat aataagcagg
     7321 ataatcatcc ccacgcgcaa tcgatccatt tcctctggaa taggcataga cactatcacc
     7381 agctgctagc atcaatccta ctactaaaaa aggaacaagc tttttaactg acatcttcaa
     7441 gtaataactt gttcctcttg ctataaacgg taaaattttc attcttcctc cttaaaaaat
     7501 aatataaagc gatgattgcc ttcacctaaa ttattcggag aaaaaagaaa aagtgagaaa
     7561 atttctcact ttagtcaaga taatgaatca aattcttttc tgtaagaata tctgagtaag
     7621 taaaggattc aacgtaaaca ttaacttgtt tatctccttc cacatcccca aactccacaa
     7681 taaataggga tggataaact tcaattagct tacccaatct atttttttgg cgcttacgac
     7741 cattctccaa agtcatttct acgacttgtc cctcatgtgc cttgatttct tctttgattt
     7801 ttttcatctt ggctacatct gtaaatgcat ctgacatctt atgcctccct ctttgagata
     7861 cttgaaaatt tattgtattc tttttggaaa atcaattcca ctgttccacg agctccacta
     7921 cggtttttct cgataataac ttccacctta ttatttggga taccctcctc ttcttcacca
     7981 ccacgttcat agtagtcatc gcgatagaga aaagctacga tatcagcgtc ctgctcaata
     8041 gacccagatt cacgaatatc agacaagacc ggtctcttgt cctgacgttg ttctacacca
     8101 cgagaaagct gactcagagc gattactgga accttcaatt ccttggctag tattttcaac
     8161 tgacgagaaa tttcagaaac ttcttgttga cgattttctc gaccagttcc cgtgataagt
     8221 tgcaaatagt ctatcaaaat caaaccaaga tttccagttt cttgagccaa tttacgagaa
     8281 cgagaacgaa tctctgtaat ccgaatacct ggcgtatcat cgatatagat actggcgtta
     8341 gctagattac cctgagcaat agtatatttt tgccactcct catctgtcaa ttgccctgta
     8401 cggatagaat gtgactccac caagccttct gcagctaaca tacggtctac caagctttcc
     8461 gcacccattt cgagtgaaaa aatagcaacc gttttgtcca acttagtccc aatattctga
     8521 gcgatattca aggcaaatgc tgtcttacca actgctggac gagctgctaa gataatcaac
     8581 tcctcctcat gaagtcctgt tgtcatatga tccaaatcac gataacctgt cgcaatacct
     8641 gtaatatcgg tcgtttgttg cgagcgagct tccagatttc caaagttgag attcaacaca
     8701 tctcgaatgt tcttaaaccc gcttcgattt gcattttcac tgacatcaat cagccctttt
     8761 tctgcctgag caataatttc atcagctggt tgtgacgctt cgtaagcttg gttgacagac
     8821 tctgtcaact tggcaattaa acgacgtagc attgcttttt ctgcaacaat cttagcataa
     8881 tactccgcat tagcagaagt tggcacagaa ttgacaatct caaccaagta agacaagcca
     8941 ccaatattct gtaaatcacc ttgattatca aggatagtac gaaccgttgt tgcatctatg
     9001 gcatcaccac gatcggataa atcgaccatg gcttggaaaa tcaaacgatg ggcatactta
     9061 aaaaagtccc gagactcaat gtattctcgc acaaaaacaa gtttactctc atcaataaag
     9121 atagccccta aaacggattg ctcagctaag atatcttgag gttgtactcg taactcttct
     9181 acttctgcca tcagacttcc cttcctttta caatcttgtc aagaaggtgt aaacttatcc
     9241 ttctttcaca cgaagattga ttacacttgt gatatcttga tagattttca ctggcacatc
     9301 aatcaaacca accgctcgaa tcggagcttg tacttgaata tgacgtttat caatcttaat
     9361 tccaaattgc ttttgcaatt cttctgcaat cttcttattg gtaatagaac caaaggtacg
     9421 accatctgga ccaacttttt caacaaattc tacaacagtt tcttctgctt caagttgtgc
     9481 tttaattgct tttccttctg caatcatctc agcgtgagct ttttcttccg atttttgttt
     9541 accacgaagt tcacctacag cttgagcagt cgcttctttg gctagattct ttttgataag
     9601 aaagttttgc gcataccctg ttggtacttc cttaatttcg ccttttttac cttttccttt
     9661 aacatctgct aaaaagatta ctttcattct tctttctcct tttcctttat ttcatttaat
     9721 acaatttctg tcagtttttc acctgcttct gacaaggtta catctttaat ttgagctgct
     9781 gccaaattaa agtggcctcc accgcctaac tcttccataa tccgttgtac attcagttta
     9841 ctacgacttc gagctgagat agagataaat ccttgtgtat tcttcgcaag aacaaaactc
     9901 gcttcaatac ctgacatggc taacatggca tctgctgcct tactaataac aactgtatca
     9961 tagcatttcg tgtccttagc ctctgctatt agtacatctg aacctaattt acacccctgt
    10021 aaaataagtt cattgacctc acgatattct tcaaaatctg tcgcagcgat ttcctggata
    10081 gcaatactat cacttccgcg cgttctgaga tagctagcaa catcaaatgt ccgactagtt
    10141 actcgcgagg tgaaattttt agtatccaac atcataccag ccatcaaaac acttgcttgc
    10201 atacgactca aacgattttt cttagaattc tggaactgaa tcaattccgt taccaactca
    10261 ctggcactac ttgcaccact ttcgatataa gtaataaccg cattatctgg aaaatcctga
    10321 tcccttctat ggtggtcaat aacaatggtt tgggtaaata aatcataaaa ttcttttgat
    10381 aatgttaagg ctgtctttga atggtctaca agaatcaaca aagaacgatt ggtcaccatc
    10441 cccattgcat ccttaacaga caacaacttc gtaactcctt ctttttctat gaatgaaaca
    10501 gctcgttcaa tatctggaga catttgttct tcatcataaa gagcatagct attttcaatc
    10561 acattgctgg cgaacaactg catacctaca gcagagccca aagcatccat gtctaaattt
    10621 ttgtgaccga ctacaaaaac ctgatctaca ctccgaatct tatctgaaat agctgtcatc
    10681 atagcgcgcg tacgagtccg tgtacgcttg attgaagcag cagacccacc accaaaataa
    10741 actggatttt tcgtttcgtc gttttcctta acaaccacct ggtcgccacc acgtacttca
    10801 gccaagttca aattgagcaa agcaactttc cctatctcat catgatttcc atcgccataa
    10861 gaaaatccca tacttaaggt caagggcaac tgtctctgtt tcgactcttc tctgaaagca
    10921 tcaataacag aaaatttatc attcatcaag ccctcaagca ccgtgtagtc agtaaataga
    10981 taaaatcgat ccatacttac ccgacgagaa aacatcatgt atttttctga aaactctgat
    11041 ataaaattag ctacaaaact attgatttga ctaatatctg actcagaagt tgcatcctcc
    11101 aaatcatcat aattatccac agagacaatc ccaatcactg gtctacttgt taccaattca
    11161 tctgttatgg cttgttccct ggatacatct acaaaataca aaacaccgga agaagcatcc
    11221 atatgaacag cataacgctt ctcaccaagc ttggcataag tagacggatt tcctactgaa
    11281 gccttgataa tcgtttgaac agcttctaaa tcaaaatcac catcttcctt ggtcaaaatc
    11341 aattcagcat agggattaaa ccactcaacc tctccagaag ataaattcaa tttcataaca
    11401 cctacaggca tctgttccaa tagagctgtc aaactttctt ccgcttggtg gtttacatac
    11461 tgtatctgtt ctacatcact ccttgtataa tgcactctca gtttcttaaa taaaaaaaca
    11521 tagcctccta caaaaagaaa caaaattaaa accgtcaaca gattattatt aacaaaaata
    11581 atgaaagtgg ataagactcc aaacgcaatc aatcctacta gaataggaaa aattggactt
    11641 acataaaatt ttttcattca aaacctcttg gcacccatta taccataata cccctcaaaa
    11701 agcgactttt taaaagtgta atcagtaatt ctatcaatta taagaaaaag gtagtttaca
    11761 attcagtaaa cctaccttta cacatattga aattaagatt ctttaacctc taacaaacca
    11821 atttcgccat cctcacgacg ataaatcaca ttggttgtct gatcttcaac atccacatag
    11881 ataaagaaat catgccccaa taaatccatt tgtagaattg cttcttccaa atccattggt
    11941 tttaaatcaa tttgttttga acgaacaact ttagactgga caatatttga atcttccacc
    12001 aaagcatctg taaataattg accagttgct accttatttt tatttttacg ctcgattttt
    12061 gttttatttt tacgaatctg acgttcaatt ttatcagtta caaggtcaat tgaaccatac
    12121 atatcttgag atacatcttc tgcgcggaga gtaatagatc caagcggaat cgttacttcc
    12181 actttagccg ttttttcacg ataaactttt aagttaattc gggcatccaa ctcttgttct
    12241 ggttggaagt acttttcgat cttttcgagt ttagaaacta cataatcacg aattgcttct
    12301 gttacttcta ggttttcacc acggatacta tatttaatca tatgagtacc ttctttctaa
    12361 acatttttgt ttttatgatt ttattataac gctttcattc tatttttgca aattttttcc
    12421 tcatcttaca agggaaaatg tttttacatc cttagcacca gcttcttcca acagtttctt
    12481 aacacgattt atagttgctc ctgtagtata gatatcatct ataagtagga tttttttagg
    12541 aatagtgact ccacttttaa taaagaaagg aagttctgtc cccaagcgct ctgaacgatt
    12601 tttagaagaa ctggctctct cttctctttt ctctaataaa tccagatact caaagcctgc
    12661 tgcctctacc aagccctcaa cctgattaaa tcctctatta gcatatctat caggacttag
    12721 gggaattaca acaaattgat actctttgta ctttttcaac tcctcactta aaaatgaagc
    12781 gaaaactttt cttaacagga agtctccatc aaacttatac cgactgaaaa aatccttcat
    12841 agcttgattg taagtaaaaa tcgctctatg actgacttca actccttctt tacaccaaag
    12901 ttgacaatct tgacactttg ttgacaactc tgttttcata caatttggac agttctcttc
    12961 cccaattctt tcaaaagtag aatcacagtc tgaacaaaga caagagtcat cattcctcag
    13021 aagtaagaga ctactaaaag ttaaaacagt cttcatagtc tgcccacata acaagcactt
    13081 catagaccag cctccttatt catcatctga atttccttaa tcgccttctt gattgaagca
    13141 tttaacccat catggaagaa aagcaaatct cctgtcggtc tatccatgct tcgtccaact
    13201 cgtccaccaa tctgaatcaa actagacttg gtaaacaaac gatgattggc ctctactacg
    13261 aaaacatcca cacaagggaa ggtaactcca cgctccaaga ttgtcgtact gataagtatt
    13321 gtcagttctc catctcgaaa agcttgtact tgctctaatc gatcctctgt tacagaagat
    13381 acaaagccaa ttttctcatt tggaaattgc tcctgtaaga tttcttctaa ctgctcccct
    13441 ttcttaattt ctgaagcaaa aatgagtaac ggataagctg tctttctctg cttctcaata
    13501 taggacttta actttggtga caaacgattc ttgtctaagt agcgattaaa atccgataac
    13561 caaattggtt ttggaataat caacggattt ccatgaaacc gtctcggtaa attcagtctt
    13621 tttagttctc ctaaacggac ctttttatct aactcattgg tcgaagtcgc tgttaaaaag
    13681 attctcaatc cattctcctt tacactattc ttgacagcat ggtaaagcgt gggattatca
    13741 acataaggaa aagcatctac ttcatccact atcagcaaat caaaagcttg ataaaacttc
    13801 aataactgat gggttgttgc aacaactagt ggtgttcgaa aataaggttc cgattctcca
    13861 tgtagcaaag ctatcccgca agaaaaatcc tgttgcaggc gcttgtacag ctccaaacaa
    13921 acatctatgc gaggactagc caaacacact gcaccacccg cattgatcac tttagccact
    13981 acttgataaa tcatttctgt ctttccagct cctgttaccg catgaactaa ggttggcttt
    14041 tgcttgtcta ctgcttgaag cagtccctct gacaccttct cttgaaaagg agttaattgg
    14101 ccgcgccatt tgagaacatc ttgcttcgga aaatcctcct gcggaaaata gtataaagtt
    14161 tgatcacttc tgactcgctt catcagcaag cactctcgac aatagtaagc accgatgggc
    14221 aaataccatt ctgctagaat agtgctatta caacgttgac agaaaagttt ccccttctcc
    14281 tttctcattg ctggaagttt ctccgccaac tgacgttctt cttctgttaa ttcattctca
    14341 gtaaataaac gaccgagata atctaaattt actttcatac ttctttattc gtaaaaacta
    14401 gcactttaga tgatttttta gtacaattaa atcatggaat ttaggacaat taaagaggac
    14461 ggccaagtcc aagaagaaat caaaaaatct cgctttatct gccatgccaa gcgtgtttat
    14521 agcgaagaag aggctcgtga cttcattact gccatcaaaa aagaacacta caaagcgaca
    14581 cataactgct ctgcctttat tattggagaa cgtagtgaaa ttaaacgtac aagtgatgat
    14641 ggtgagccta gtggtactgc tggtgttccc atgcttgggg tactagaaaa tcacaatctc
    14701 accaatgtct gtgtggtcgt gacacgctac tttggtggta ttaaactagg cgctggagga
    14761 ctaattcgtg cttacgccgg cagtgtcgcc ttagctgtca aagaaattgg tattattgaa
    14821 ataaaagaac aggctggcat tgctattcaa atgtcttatg ctcagtacca agagtacagt
    14881 aacttcctta aagaacatga tctcatggag ctggatacaa actttacaga tcaagtcgat
    14941 acgatgattt atgttgataa agaagaaaaa gaaactatta aagctgcact tgtggagttt
    15001 tttaatggaa aagtcacttt aactgaccaa ggtttacgag aggttgaagt tcctgtaaac
    15061 ttagtgtaaa caatgaataa tacagcgttt cgttgacatt ctcacaacta ctttagcgag
    15121 caaaataaaa agatgcgtac caaaatatac tagaaaatga agcaattcaa acgaaacctg
    15181 atatcgtttt ccttcacacc tatttactag aattaactga acgcaatcac ttgaaaatta
    15241 atgactttga tctatgatat atagaaatcc tatcgcttcg ataggatttc tatattttac
    15301 aaatggtatg gatagcgtta tactaaagat atcttataca aagaggtatt catatgtcta
    15361 tttataacaa cattactgaa ttaatcggtc aaacaccgat tgttaaactt aacaacatcg
    15421 tgccagaagg tgctgcagac gtctatataa agcttgaagc atttaatcct ggttcatctg
    15481 taaaagaccg tattgccctt agcatgattg aaaaagctga acaagatggt attctgaaac
    15541 ttggttctac tattgttgaa gcaacaagtg gaaacaccgg tattggactt tcatgggtag
    15601 gtgctgctaa agggtataaa gtcgtcatcg ttatgcctga aactatgagt gtagaacgac
    15661 gtaaaattat ccaagcttat ggtgctgaac tcgtcctaac tcctggtagc gagggaatga
    15721 aaggtgctat tgctaaggct caagaaatcg ctgctgaacg tgatggtttc cttcctcttc
    15781 aatttgacaa tccagctaat ccagaagtac acgaaagaac aacaggagct gagatactag
    15841 ctgctttcgg taaagatgga ttagatgcct ttgttgctgg agtaggtact ggtggaacga
    15901 tttctggtgt ttctcatgca ctcaaatcag aaaattctaa cattcaagtt tttgcagtag
    15961 aagcagatga atctgctatt ctatctggtg aaaaacctgg tcctcacaaa attcaaggta
    16021 tctcagctgg atttattcct gatacacttg atactaaagc ctatgatggt atcgttcgtg
    16081 taacatcaga tgacgctctt gcactcggac gtgaaattgg tggaaaagaa ggcttccttg
    16141 tagggatttc ctcagctgca gctatctacg gagccatcga ggttgccaaa aaattaggta
    16201 caggtaaaaa agtccttgcc ctagcaccag ataacggtga acgttatctc tctacagcac
    16261 tttatgaatt gtaaccgtcc aataacgaag tctattgaaa aatctccaga ctagagaact
    16321 cacggatagt tcctaatctg gagatttctt atttgcactt ttcttgtaca actttagtcc
    16381 atggtaaata ggcctctaaa acctctttgt ttacgagagt ttccacgttt ggaagacatt
    16441 ctagaagata ggatagatat ttctcactat ttataatgga ttgaaataag atatgaacaa
    16501 atcgattagg acatgatggt aaagcgtaat cccttgtttc tcagctttcc cagacaaaaa
    16561 agtccaatag taagtcagct gactatcact ctctagcacc ctataagaag tttcatccgc
    16621 atgaagtaag ggctgagtca atagtctctc tcgcaagagg ttataaaggg gctccaaata
    16681 gtattgactc gtcttgatat gccgattaga gatttcctta cgtgtgattg gtaaacccat
    16741 cctagcccaa tcttcttctt ggcgataatt gggtaccttc agattaaact tctgatggat
    16801 ggtgtgagcg ataatagaag ctgagccaaa gttatgcgct aaaggggctt taggaatagg
    16861 agatttcaca agcttatcca gatgattatc ttttactcgt tatggacaat gctatatggc
    16921 ataaatcaag taccttaaag attccgacta atattggctt tgcatttatt cctccataca
    16981 caccagagat gaaccccatt gaacaagtgt ggaaagagat tcgtaaacgt ggatttaaga
    17041 ataaaacctt tcaaactttg gaagatgtca tgaatcaact ccaagatgtt atacaaggac
    17101 tggagaagga ggtgataaag tccatcgtta atcggagatg gactagaatg ctttttgaaa
    17161 acagatgagt ataaaaagaa agtcctcatt tcaatagaaa tcacgacttt ctgatggatt
    17221 tatagtaaaa tgaaataaga acaggacaaa tcgatcagga cagtcaaatc gatttctaac
    17281 aatgttttag aagcagaggt gtactattct agtttcaatc tactatattt ttggagtgat
    17341 agaaaagccc ttcataagcc agtctacttg ttcaggtgtg agagctttga catccttttc
    17401 tgtactgggc caagtcagtc tg
//
LOCUS       DAVMMR010000044        16415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMMR010000044 DAVMMR010000000
VERSION     DAVMMR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.044"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            141..1586
                     /locus_tag="VYS74_001458"
     CDS             141..1586
                     /locus_tag="VYS74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3789481.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            1588..2319
                     /gene="cps4B"
                     /locus_tag="VYS74_001459"
     CDS             1588..2319
                     /gene="cps4B"
                     /locus_tag="VYS74_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV3789482.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2328..3020
                     /gene="cpsC"
                     /locus_tag="VYS74_001460"
     CDS             2328..3020
                     /gene="cpsC"
                     /locus_tag="VYS74_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV3789483.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            3030..3719
                     /locus_tag="VYS74_001461"
     CDS             3030..3719
                     /locus_tag="VYS74_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV3789484.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            3734..5095
                     /locus_tag="VYS74_001462"
     CDS             3734..5095
                     /locus_tag="VYS74_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3789485.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGSSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            5101..5844
                     /locus_tag="VYS74_001463"
     CDS             5101..5844
                     /locus_tag="VYS74_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV3789486.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            5841..6665
                     /locus_tag="VYS74_001464"
     CDS             5841..6665
                     /locus_tag="VYS74_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV3789487.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            6667..7545
                     /locus_tag="VYS74_001465"
     CDS             6667..7545
                     /locus_tag="VYS74_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3789488.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            7546..8880
                     /locus_tag="VYS74_001466"
     CDS             7546..8880
                     /locus_tag="VYS74_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV3789489.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            8908..10332
                     /locus_tag="VYS74_001467"
     CDS             8908..10332
                     /locus_tag="VYS74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789490.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            10397..11485
                     /gene="wecB"
                     /locus_tag="VYS74_001468"
     CDS             10397..11485
                     /gene="wecB"
                     /locus_tag="VYS74_001468"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV3789491.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            11648..12517
                     /gene="rfbA"
                     /locus_tag="VYS74_001469"
     CDS             11648..12517
                     /gene="rfbA"
                     /locus_tag="VYS74_001469"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV3789492.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12518..13111
                     /locus_tag="VYS74_001470"
     CDS             12518..13111
                     /locus_tag="VYS74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV3789493.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            13123..14172
                     /gene="rfbB"
                     /locus_tag="VYS74_001471"
     CDS             13123..14172
                     /gene="rfbB"
                     /locus_tag="VYS74_001471"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV3789494.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQVGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            14238..15089
                     /gene="rfbD"
                     /locus_tag="VYS74_001472"
     CDS             14238..15089
                     /gene="rfbD"
                     /locus_tag="VYS74_001472"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV3789495.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            15152..15486
                     /locus_tag="VYS74_001473"
                     /pseudo
     CDS             15152..15486
                     /locus_tag="VYS74_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            15753..16338
                     /locus_tag="VYS74_001474"
                     /pseudo
     CDS             15753..16338
                     /locus_tag="VYS74_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         5145 a         2711 c         3474 g         5085 t
ORIGIN      
        1 tgttcttatt tcattttact atatttttgt ttcgcgggaa gtctactaag atacttaaag
       61 atgcagatag taaaaaaaat gtagacatta ccgtaaaaaa gtgatataat cgtatgatgt
      121 tcaaggtata ggtgttaatc atgagtagac gttttaaaaa atcacgttca cagaaagtga
      181 agcgaagtgt taatatcgtt ttgctgacta tttatttatt gttagtttat tttttattgt
      241 tcttaatctt taagtacaat atccttgctt ttagatatct taacctagtg gtaactgcgt
      301 tagtcctact agttgccttg gtagggctac tcttgattat ctataaaaaa gctgaaaagt
      361 ttactatttt tctgttggtg ttctctatcc ttgtcagctc tgtgtcgctc tttgcagtac
      421 agcagtttgt gggactgacc aatcgtttaa atgcgacttc taattactca gaatattcga
      481 tcagtgttgc tgttttagca gatagtgaga ttgagaatgt tacgcaactg atgagtgtga
      541 cagcaccgac tgggactgat aatgaaaaca ttcaaaaact actagctgat atcaaatcaa
      601 gtcagaatac cgatttgacg gtcaatcaga gttcgtctta cttggcagct tataagagtt
      661 tgattgcagg agagactaag gccattgtct taaatagtgt ctttgaaaat atcatcgaat
      721 cggagcatcc agattacgca tcgaagataa aaaagattta taccaaggga ttcactaaaa
      781 aagtagaagc tcctaagacg tctaaggatc agtctttcaa tatctatgtt agtggaattg
      841 atacttatgg tccgattagt tcggtgtcgc gttcagatgt caatatcctg atgactgtca
      901 atcgagatac caagaaaatt ctcttgacca caacgccacg tgatgcttat gtaccaatcg
      961 cagatggtgg gaataatcaa aaagataaat tgacccatgc gggcatctat ggagttgatt
     1021 cgtccatcca cactttagaa aatctctatg gagtggatat caattactat gtgcgattga
     1081 acttcacttc tttcttgaaa ttgatcgatt tgttgggtgg ggtagatgtt tataatgacc
     1141 aagattttac atctctacac gggaagttcc atttcccagt tggaaatgtc cacttagatt
     1201 ctgagcaggc tctgggattt gttcgtgagc gctattccct agcggatggt gatcatgacc
     1261 gcgggcgcaa tcaacaaaag gtgattgcgg ccatccttca aaaattaact tcgagcgaag
     1321 cgctgaaaaa ttatagtagg atcattgata gcttgcaaga ttctatccaa acaaacatgc
     1381 cacttgagac catgataaac ttggtcaatg ctcagttaga aagtggtgga acgtacaaag
     1441 tgaattcgca agacttgaag ggtatgggac ggacggatct tccttcctat gcgatgccag
     1501 atagtaacct ctatatgatg gaaattaacg acagtagcct tgcatctgtc aaaactgcta
     1561 ttcaggatat gttggagggc agatgaaatg attgatattc attcgcacat cgtctttgat
     1621 gtagatgatg gtcccaagtc aagagaggaa agcaaggctc tcttggcaga atcctacagg
     1681 cagggggtgc gaaccattgt ctctacctct caccgtcgca agggcatgtt tgaaactccg
     1741 gaagagaaga tagcagaaaa ctttcttcag gttcgggaaa tagctaagga agtggcgagt
     1801 gacttggtca ttgcttacgg ggctgaaatt tactacacat cagatgtttt ggataagctg
     1861 gaaaaaaatc ggattccgac cctcaataat agtcgttatg ccttgataga gtttagtatg
     1921 aacacccctt atcgcgatat tcatagtgcc ttgagtaaaa tattgatgtt gggaattact
     1981 ccagtcattg cccacattga gcgctatgat gcacttgaaa ataatgaaaa acgcgttcga
     2041 gagctgatca atatgggctg ttacacgcaa gtaaatagtt cacatgtcct caaatccaaa
     2101 ctttttggag aacgttataa attcatgaaa aaaagagctc agtatttttt ggagcgtgac
     2161 ttggttcata tcattgcaag tgatatgcat aatgtggaca gcagacctcc ccatatggca
     2221 gaagcatatg accttgtttc ccaaaaatac ggagaagcga aggctcagga actttttata
     2281 gataatcctc gaaaaattgt aatggatcaa ctaatttagg agaaatgatg aaagaacaaa
     2341 acacgataga aatcgatgta tttcaattac ttaaaacctt gtggaaacac aagctaataa
     2401 ttttattagt ggcacttgtg acaggggcgg gagcttttgc atatagcact tttattgtta
     2461 agccagaata tacaagcacc acgcgtattt acgtagtcaa ccgtaatcaa ggagataagc
     2521 cgggactgac gaatcaggac ttgcaggcag gaacttatct ggtaaaagac taccgcgaaa
     2581 ttatcctttc gcaagatgta ttggaaaagg tagcgacaaa tttgaaattg gatattccag
     2641 caaaaacgtt aactagcaaa gtgcaagtga ctgtaccagc cgacactcgt atcgtctcaa
     2701 tctctgtcaa ggataaacaa ccagaggaag ccagtcgcat tgctaattct ctacgagaag
     2761 ttgctgcaga aaagatcatc gctgtaacgc gagtatctga tgtaacgacg cttgaagaag
     2821 cgcgaccagc tatgactccc tcttctccaa atgttcgacg caacacctta gttggttttc
     2881 ttggaggagc cgccgtaaca gtaattactg ttcttttgat tgagttgctc gatacccgtg
     2941 taaaacgtcc tgaagatgtt gaagatgtac tgcaaatgcc acttctagga gtcgttccag
     3001 attttgataa aatgaaatag gaggaagtta tgccaacgtt agaaatctca caggcgaaat
     3061 tagaacttgt gaaaaaggca gaggaatatt ataatgcttt gtgcacgaac ctacagttaa
     3121 gtggagatga tttgaaagta ttttctatca cttctgtgaa agcaggagaa ggaaaaacaa
     3181 cgacttccac caatatcgct tgggcttttg cgcgtgcagg ttacaaaacg cttctgattg
     3241 atgcagacat gcgtaactcc gttatgtcag gtgtctttaa atcaagggaa aggattactg
     3301 gactaacaga atttctatca gggacaacag acctgtcaca ggggctttgc gataccaata
     3361 ttgagaatct ctttgtgatt caggctggct ctgtgtcacc aaatccgaca gcccttcttc
     3421 aaagtaagaa tttcagtaca atgcttgaaa ccttgcgtaa atattttgac tatatcgtcg
     3481 tagacactcc ccctgtcggt gtcgtgattg atgcggctat cattatgcag aaatgtgatg
     3541 cttctatttt agtgacgaag gcaggtgaaa caaaacgacg ggatcttcaa aaagcgaaag
     3601 aacagttgga acaaactggg aagtcgtgtt taggagttgt gttgaataaa ctcgatactt
     3661 cagtagagaa atacggttct tatgggagtt atgggaatta caggaaacag aaaaaatagg
     3721 ttgagggata gaaatgagtg aaaaactagc aaagccttca gtagccatag tccagagttt
     3781 tcttgttatt ttattgactt atctacttag cgctgtgaga gaaacagaga ttgtttcaac
     3841 aacagctatt gtactttata tcctccacta ttttgtcttt tatatcagtg attatggacg
     3901 gaatttcttt aaaaggggat atttgattga acttgtccag acattgaaat atatcctatt
     3961 ctttgcgcta gcgattagta tttctaattt tttcttagaa gatcgattta gtatttccag
     4021 aagaggcatg atttacttcc tcttattaca tgttttctta gtctatgtgc taaacccatt
     4081 tatcaagtgg tattggaagc gggcttatcc caactttaaa ggaagtaaga agattctcct
     4141 acttacagca acttctcgtg ttgaaaaggt actggataga ttaatagaat caaatgatgt
     4201 tgttggggag ttggtagccg tcagtgtttt agataaacca gattttcagc atgatgattt
     4261 aaaggttgta gcagaggggg agatagtaaa ttttgcgact catgaggtgg tcgatgaagt
     4321 ctttatcaat cttccaagtg aaaaatacaa tattggagag cttgtctctc agtttgaaac
     4381 gatgggaatt gatgtaacag tcaatctaaa tgctttcgat tgggcacgta acaagcaaat
     4441 ttgtgagatg gcaggactaa atgttgtgac tttttctaca acattttata agactagcca
     4501 tgtgattgct aagcggatta ttgatattgt cggttccctg gtaggtttga tattatgtgg
     4561 tctagtcagt attgtgctgg ttcctttgat tcgaaaggat gggagttctg ctttttttgc
     4621 tcagacgcgt ataggaaaaa atggccgcca ttttactttt tataagtttc gctctatgtg
     4681 tgtggatgct gaggagaaaa aaagagaact catggaacaa aataccatgc agggtggaat
     4741 gtttaaggtg gacgatgatc ctcgtatcac aaaaattggt cattttatac ggaagacgag
     4801 cttggacgag ctaccacagt tttataatgt tctaaaggga gatatgagtt tggtaggtac
     4861 acgaccacca acagtggacg agtatgagca ctatacccca gaacaaaaac gtcgcctaag
     4921 ttttaaacct ggcataacag gtttatggca ggtcagcgga cgaagtgaaa tcaaaaattt
     4981 cgatgaagtt gtcaaattag atgtggccta tattgatggt tggacaatct ggaaagatat
     5041 tgaaatttta ttgaagacag ttaaggttgt attgatgaag gatggagcga aatagttcat
     5101 atgaatgaga gaattcaaat attaggaatt actatagatc cattaacgat gaaagaaaca
     5161 gtggatgctg ttgaacagta tgttttaaag aagcaccctt tgcacttgat gggagttaac
     5221 gcggataaaa ttaatcagtg ctatgaagat gagaaaatca aaaaaatcgt taatgaatca
     5281 ggaataatta atgccgacgg tgcttcagta gttttagcga gtaagttttt aggaacacca
     5341 gttcctgagc gagttgctgg aatagacttg atgaaacatt tactagagtt gtctaatgaa
     5401 aaaggatact cggtctactt ttttggagca aaagaagacg ttttacaaga tatgttaaaa
     5461 atttttgaag aaagatatcc agctctaaat gttgttggct atagaaatgg atatttttcg
     5521 cctgaagatg aaaaacatat tcaggaagat attaaagaga aaaaaccaga ttttgtgttt
     5581 gtaggaatta cttctcctaa gaaagaatat attatccaat cattcatgga taatggaatt
     5641 aatgctgtgt ttatgggggt tggtggaagt tttgatgtac tatcagggca tatcaaacga
     5701 gctccattat ggatgcaaaa tgctcaccta gagtggttgt ttcgtgtggc aaatgagcct
     5761 aaacgtctct ttaagcgtta ttttgtaggg aatgtttcat ttataagaaa agttttaaga
     5821 gcgaaaaggg gatataaact ttgaaccaaa cagaaatagt taaattgatt cagaaagttg
     5881 aattggatgc tataaaagag tttcaaaaaa tttgtaaaga gaacaatata gatttcttcc
     5941 ttcgtggggg gagtgtactt ggggcagtca aatatgatgg ctttattcca tgggatgatg
     6001 acatggatat tgctgtgcct cgtgaaggct acgataaact tccaggtatc tttaaggata
     6061 gaattatcgc aggcaaatat caggttcttg cttatcaata ttgcgatact ttacattgct
     6121 actttcctcg cttattcctt ttagaagatg aaagaaaacg tttgggattg ccacgaaata
     6181 ccaatttagg tttgcatttg attgatatca ttcctttgga tggtgcccca aatcattcat
     6241 ttttaagaaa gctttacttc ggtaaagtat actggtatcg ttttttagca agtttaggaa
     6301 ctacctatgt tggagaccat gtggatatgc attccgctaa gcaaaaacta atcattggtt
     6361 tctttaaaaa gctaggattt gcaaaactat tccctcaaaa ttatgtgtac agacgcttgg
     6421 ataatctcta taaaaagtat gattggaaaa agcagaagta tgctgggaca attaatgcat
     6481 ctctgtttgc caaagaagtc atgccagtag agatttgggg agaaggagta gagaagcctt
     6541 ttgaggatgc cttctttaaa gttcccacgg agtatgaccg ctatctgaaa agactttacg
     6601 gagaaaacta tcttcacgaa gagcctagtg atgatgaaaa gaaatcgcat ttaggaggac
     6661 aataatttgt tttgttatat tattttgcat tacaaagtct tagaggaaac tatttcttgt
     6721 gttaaatcaa taaaagaagg caattccaat acaaagcaga tcgttattat tgataatttt
     6781 tctaataacg gtacgggtga aaaactacaa gagctgtatg agtcagattc agagattgat
     6841 gtcttgatta accatgaaaa tgctggtttc gctcgaggta ataatgtagc atatcagttt
     6901 gctaaggaaa agtacaaacc tgattttatg gttatcatga ataatgatat tgagatagaa
     6961 acagaagagt ttgaaaaaat cgtgacagat atctatcgga aggaaaaatt ccatttgtta
     7021 ggaccagata tcttttcgac gacgtatcag cttcaccaaa acccaaaacg gttgacgcat
     7081 tatacttatg aagaggttaa ggctctcaat gaaaaattta agaaagggag ccaagttagt
     7141 ctagcattaa aaattaaatg ttggttgaag tctagtaaag ttcttcggac agcaatctat
     7201 caaaataggc gtaaaaagaa atcagtagac tatagaaaac aggtagaaaa cccaattctt
     7261 catggttcgt ttattgtata ttctagagat tttattgaga aagaggagta tgcttttaat
     7321 cccaatacct tcttctatta tgaaacagag atattagatt atgaagctga gttaaaagga
     7381 tataagagaa tttatacacc gaagattaag gtcttgcacc atcaaaatgt tgcaaccaat
     7441 caagtctata caaacttagt agaaaaaacc ttgttttcaa acaaatgcaa ctttgaatcc
     7501 actagttatt ttttgaagtt gatgaaagaa aacgagggtg tgtaaatgac ttatttattt
     7561 ttactctgcc tgaccttatt cttattaact ttcttctatt tttttgcttt caatcaagat
     7621 ttgatagctc cgccagttgt catgtctgtt atgtttctca ttagttctgt atttgccctt
     7681 attaatgtgc aaaactggaa tattgagtat agtggtttag cttatctttt gattattagt
     7741 ggcattattg ttttttcaat gcctctcttg gctcttaatt cgcctagtct aaataccaag
     7801 attaaagtga cggatcggct cattgatatc caattctgga aaatagctct tactattata
     7861 gttgacctcg ttattctata tctttatagg agagagattc ataatcttgc acttagccat
     7921 ggttatacgg gttcaaattt tcagtggttc tttagaaatg ctaccagtta tgaaggtgag
     7981 ctaacagtgc gaacttcgat tcgggtcctc attcgtatca ttgacgtatc tgcttatatt
     8041 tttggatata cttttattaa taatttcttc atttatagtc ataaacgctc taaagattta
     8101 ctgctcttag ttccattctt gatttttatt tctaaaacct tattatctgg gggtagattg
     8161 gatattataa aaattttaat tgcgtatgtt gtaatggcct atattcagca aaaacgaaaa
     8221 gttggctggg ataaggtcat ctcccataaa tatatgagac ttggttttgt aggcttgata
     8281 gctgggattc ctacctttta ctattcttta ttcttatctg gacgttctac gactagaact
     8341 gtatttgaaa gtatttcaac ctatttagga ggttcgattc agcattttaa tcagtatatt
     8401 caaaatccta ttggagtagc tgaggttttt ggcgatgagt catttgtagc tattatgaat
     8461 attttgggta atcttggctt tgtcaattac aatagtaccg ttcacttaga atttcggcag
     8521 ttagggatta ctatgggtaa cgtttatact ttctttagaa gaccctggca tgactttggg
     8581 ttagtcggta tgtatatctt ttcctttgtt gtcggtgtgt tttttgctat tttttatttg
     8641 aaattaagaa agagccgtgc tggttttaaa ttggatattc atacaattat ttactcttat
     8701 ttcttttatt ggattttttt atcgtccatt gagcaatact cgtttacaac aatcagtttg
     8761 tttacacttg tatttatagt cttggtttac ctaatggctt tcttctattg gaatttggat
     8821 ttccacagag gaaaactagt aattaaactc tctgacacaa gtattaaatc ggaaaactga
     8881 aaacaataaa aatagagagg atagttgatg aatagcaaaa ttaaaaatat actaactaat
     8941 ttctcttatg tcatttcttc aaatcttttg acagtcttga cctcttcctt ggttgtctta
     9001 attttcccca aattaatggg ggtaacagag tacagttact ggcaacttta tattttttat
     9061 ctgacctata tcggtttttt ccacttgggt tggattgatg ggatttatct taaatatggt
     9121 ggcttagaat ataataattt agatagaaaa cagttttatt ctcagatgat tctattttct
     9181 agcttcttaa tgattatctc cttgctattg tttggtttaa atgtattagc tgtaacagat
     9241 tcgaatgcac gatatattta taatatgact attataagta tgataatcac aaatttaaga
     9301 acgctctacg tgtatgtttt gcagatgaca aaccgcttga aggatagttc catcattcta
     9361 ctcagtgatc gcgttttata tgtgctcctt ttattcctgt ttattgtatt tggttggcat
     9421 gaatacaagg ttatgatttt ggcagatatt ctaggtcgat cattttctct catactttcc
     9481 ttctggattt gtaaagatat tgtgtttcag cctttgtcaa aattcatctt taatataaaa
     9541 gagtcttttg ataatatccg agttggtatc aatttaatgt tatccaacat cgcaagtagt
     9601 ttgattatcg gtattgttcg tctaggaatc caatggaact ggaatatcga aacattcggg
     9661 aaagtatctc tgaccttgag tgtttccaat ctattgatga ctttcattaa tgccattgga
     9721 ctagtcgtct ttccgctatt aagacgaaca aaagcagaaa acttacctaa gatttattct
     9781 aatttaagaa acgttttgat gcttatcatg ttcgcgattt tgctcattta ctatccttta
     9841 aaaattgtat tagacctctg gttgccagcc tatcaagatg ccttgatttt catgaccctt
     9901 attttcccta tgtcagtcta tgaagggaaa atggcattgg tcattaatac ttacttaaag
     9961 gcattaagaa tggaaagaga tatccttaga gtcaatgcct tagtaatgtt gatcagtatg
    10021 ggagtgaccc tggttacaac atacctgtta aatagtttgg agctgactgt tgtatcgata
    10081 gttgttttgc tagctttgag aagtatcata gccgaattaa ttctgtctaa aaaactggat
    10141 gtttcggtta agaaggatat tgtattagaa tttcttttga cgcttgtctt tatttcttca
    10201 agttggtatt tgccgattgg gctggcagta attgtctaca caatagccta cggtttatat
    10261 ctctatttga aacacgaaga tatcaaaacc tatttagctt actttaaagc tagtaaaaaa
    10321 acatcaaatt aaaaaattat ataatgatta agtggtagat tctatttcta ccgttttaga
    10381 tattcgggag gtaatgatga aaaaaattat gttagtattc ggtacacgtc cggaagccat
    10441 caaaatgtgt ccattagtca atgagttgaa aaaacacgaa gatatggaaa caattgtgtg
    10501 tgttactgga caacacaaag agatggttag tcctgtttta gatttatttg gtgttgtacc
    10561 agattatgat ttagaaatta tgaaggctaa ccaaaccttg ttctctatca caactagtat
    10621 cttggaaaag ataaaaccag ttttagagaa ggaacaacca gatattgtcc tagttcacgg
    10681 tgacactacg acaacttatg cagcagcctt ggcagcattc tatttgggaa ttaaagtagg
    10741 acatgttgaa gctggtttgc gaacgtacaa tttacaaagt ccatttcctg aagaatttaa
    10801 caggcaatcg acatcaatca ttgcaactta ccattttgct ccaactgagt tggctaaaga
    10861 aaatctctta aaagaaggta gagagaatgt ttatgtgact ggaaatactg tcattgatgc
    10921 tcttacaact actgttcaag aggattatac acacactcat ttagatttaa acgctaacaa
    10981 tcgtctcatc ttattgactg ctcatagacg cgaaaatctc ggcgaaccga tgagacatat
    11041 gtttagagca gttaaacgag tattgaatga atacgaagat gttaaagtca tttatccaat
    11101 tcataagaat cctttggtac gtgaaacagc tgcagagatc tttggagata cagaacggat
    11161 tcaaattatt gaacctttgg atgttcttga tttccacaac ttcatgaacc aaagttatat
    11221 gattttaaca gattctggcg gagttcagga agaagcacct tctttaggaa aacctgtatt
    11281 ggttatgcgc gatactactg aaagacctga aggagtagcg gcaggaacct tgaaattagt
    11341 aggaactgac gaagaaacaa tttatcaaaa cttcaaacta cttctagatg attctggaga
    11401 atacaaaaaa atgagtcagg ctagcaatcc atacggtaat ggtgatgcta gtcaacagat
    11461 tgttcagatt ttgcgtggga tttaagaaga ttctctaaaa gagtctaaga ggaatccact
    11521 ccacaaacat aaaactctta tgctatcatt tatgattgag atagactcat aacttaatat
    11581 ttttatccta agaaaattcg ttcatattca atatgaatga aaaacaacga agaaaggtta
    11641 tttcattatg aaaggtatta ttcttgcagg cggctcaggt acccgcctgt acccacttac
    11701 tcgggctgcg tcaaaacagc tgatgccggt ttatgataaa cctatgattt attatccgtt
    11761 gtcgacatta atgttggctg gaattaaaga tattttgatt atctcaactc ctcaagattt
    11821 gccccgtttt aaggacttgc tcttggatgg ttccgaattt gggatcaagc tttcctatgc
    11881 ggaacaacct agtcccgatg gacttgctca ggcttttctt atcggtgaag aatttatcgg
    11941 tgacgatagc gttgccttga ttttaggcga caatatctac catggtcctg gtctgagcac
    12001 aatgcttcaa aaagcagcca agaaagagaa aggtgcgact gtttttggct accaagtgaa
    12061 ggatccagag cgttttggtg tggttgagtt tgatacagac atgaatgcta tttctataga
    12121 agaaaaaccg gagtatcctc gctccaacta tgcagtgaca ggactgtatt tctatgataa
    12181 tgatgttgtg gagattgcta aacagatcaa acctagtgct cgtggcgagt tagaaattac
    12241 agacgttaac aaggcttacc taaatcgtgg tgacctttct gttgagctga tggggcgtgg
    12301 ttttgcctgg ttggatacgg gaacccatga aagcttgcta gaagcttctc agtatattga
    12361 aacggttcag cgtatgcaga acgttcaagt ggcaaatcta gaagaaattt cctatcgtat
    12421 gggctacatt agtcgtgaag acgtgctgga attggctcag cctcttaaga aaaatgaata
    12481 cggacaatat ttgctccgtt tgattggaga agcatagatg tcagataatt tttttggaaa
    12541 gacacttgtg gtacgcaaga ttgatgctat accaggactg ctagagtttg atattcccgt
    12601 tcatggagac aatcgtggtt ggtttaagga aaatttccag aaggaaaaga tgctaccgct
    12661 tggttttcct gaaagcttct ttgctgcagg gaaactgcaa aataacgtca gcttttctcg
    12721 caaaaatgtt cttcgaggac tccatgctga accttgggac aagtatatct ctgttgcaga
    12781 cgatgggaag gttttaggat cttgggtaga tttgcgtgaa ggcgagacct ttggaaatgt
    12841 ttaccagaca gagattgatg ctagcaaggg aatttttgtc cctcgtggcg tagccaacgg
    12901 ttttcaagtc ctctcagata cggtttccta tagctatctg gtcaatgact actgggctct
    12961 tgaactcaaa cccaaatatg cctttgttaa ctatgctgac ccagcattgg gaattgagtg
    13021 ggaaaaccta gcggaagctg aggtttcaga ggcagacaaa caccatcctc tattaaggga
    13081 tgtcaaacca cttacgaaag acgagttgta aaaggaaaca ttatgactga atacaaaaaa
    13141 attatcgtga caggtggagc tggttttatc ggttctaact ttgttcacta tgtttacaat
    13201 aactttacag atgtccatgt gacagtgctg gacaagctga cttatgcagg taatcgtgcc
    13261 aatattgaag aaattttagg cgaccgtgtt gagttagtcg ttggagatat tgctgatgca
    13321 gccttggtag ataagttagc agctgaagcg gatgccattg tacactatgc ggcagaaagc
    13381 cacaatgaca attcgcttaa tgacccctcg ccatttatcc ataccaactt catcggaact
    13441 tatacactct tggaagcggc tcgtaaatac gaccttcgtt tccaccatgt gtcgactgac
    13501 gaagtctatg gggatctgcc gctgcgtgaa gatttaccag gtcatgggga aggaccaggt
    13561 gagaaattta cggctgaaac caagtacaat ccaagctcgc cttactcatc gactaaggcg
    13621 gcttcagact tgattgtcaa agcttgggtg cgctcatttg gtgttaaagc aacgatttct
    13681 aactgttcaa acaactatgg tccttaccag cacatcgaga agttcattcc gcgccagatt
    13741 actaatatct tgagcggtat caagccaaaa ctctacggag aaggtaagaa tgtgcgtgac
    13801 tggattcata ctaatgacca ttcatcaggt gtttggacga ttctgaccaa gggtcaagtc
    13861 ggtgaaacct acctgattgg tgcggatggt gagaagaaca ataaggaagt gttggaactc
    13921 atcctcaaag aaatgggaca gccagctgat gcttatgacc acgtaacaga tcgagctggt
    13981 cacgacctcc gctatgccat cgatgcaagc aagctccgtg atgaactagg atggaagcca
    14041 gagtttacca actttgaagc aggtctcaaa gcgactatca agtggtacac agataaccaa
    14101 gactggtgga aagcagaaaa agaagctgtt gaagccaatt atgctaagac tcaggagatt
    14161 attacagtat aaaaagcagg aaatcgctgc tttttattgc tatattggga agagttacat
    14221 attagaaagg tctagagatg attttaatta caggggcaaa tggccaatta ggaacggaac
    14281 ttcgctattt attggatgaa cgtaatgaag aatacgtggc agtagatgtg gctgagatgg
    14341 acattaccaa tgaagaaatg gttgagaaag tttttgaaga ggtgaaacca actttagtct
    14401 accactgtgc agcctatact gctgttgatg cagcagagga tgaaggaaaa gagttggact
    14461 tcgccatcaa tgtgacgggg acagaaaatg tcgccaaagc atctgaaaag catggtgcaa
    14521 ctctagttta tatttctacg gactatgtct ttgacggtaa gaaaccagtt ggacaagagt
    14581 gggaagttga tgaccgacca gatccacaga cagaatatgg acgcactaag cgtatggggg
    14641 aagagttagt tgagaagcat gtttctaatt tctatattat ccgtactgcc tgggtatttg
    14701 gaaattatgg caaaaacttc gtttttacca tgcaaaatct tgcgaaaact cataagactt
    14761 taacagttgt aaatgaccag tacggtcgtc cgacttggac tcgtaccttg gctgagttca
    14821 tgacctacct agctgaaaat cgtaaggaat ttggttatta tcatttgtca aatgatgcga
    14881 cagaagacac aacatggtat gattttgcag ttgaaatttt gaaagataca gatgtcgaag
    14941 tcaagccagt agattccagt caatttccag ccaaagctaa acgtccgcta aactcaacga
    15001 tgagcctggc caaagccaaa gctactggat ttgttattcc aacttggcaa gatgcattgc
    15061 aagaatttta caaacaagaa gtgagataag tagtagaatg attttctagt ctaataaaag
    15121 aggcagataa tgaactcaaa aggagcttaa ggtgtacgat tatcttgttg ttggtgctgg
    15181 tctctttggt gcagtctttg cccatgaagt agccttaaaa ggaaaaaagt aaaagtcatt
    15241 gaaaaacgga atcatatcgt gggtaatatc tatactcgtg aagaggaagg aattcaagtt
    15301 catcaatatg gtgctcatat cttccatact tctgataagg agagctggga ttatgtaaat
    15361 cagtttgcag ggtttaaccg ttacaccaat tctcctgttg caaactataa gggtgagatt
    15421 tataaccttc cttttaatat gaatactttc aataaactct ggggagttgt aacgccagca
    15481 gaagcacaag ctaagattga ggaacaacgt gctattttaa atggtaaaac tcctgaaaat
    15541 ttggaagaac aggcgatttc tcttgtaggt acagacatct acgaaaaatt aatcaaagac
    15601 tatacagaga aacagtgggg caaaccaact actgaacttc catcctttat tattcgccgt
    15661 ttaccagtac acctgaccta tgataacaac tattttaacg atacctatca agggattcca
    15721 attggtggat acactcaaat agttgaaaaa tgttggatta tgaaaatatt gatgtagaaa
    15781 caaatgttga tttctttgtg aacaaagagc aatatctgaa agattttcct aagattgtct
    15841 ttactggtat gattgatgaa ttctttgact ataagttggg cgaactagag taccgtagtc
    15901 ttcgttttga aaatgagacc ttggatatgg aaaattacca aggaaatgca gttgtgaact
    15961 atacggatgc agaaacccca tatactcgca ttattgaaca caaacatttt gagtttggga
    16021 gtcaagcaaa gactatcatt actaatagaa cattctaaaa catgggaaaa aggtgatgag
    16081 ccttattatc cagttaataa tgatcgtaat aatcatttgt ataaatcata taaaaaactt
    16141 gctgatgagc aagggaatgt tatctttggt ggccgcttag gacactatcg ttattacgat
    16201 atgcaccaag taattggagc agctttgcag tgcgtgagaa atgagttaga ttcatactca
    16261 atgaaaatca aagagcaaac taggaagcta gccacaggtt gctcaaaata ctgttttgag
    16321 gttgcagatg gaagctgacg cggtttgaag agattttcga agagtataaa caagtaaaac
    16381 tgactaccag ttattattta gaaatagtat taaaa
//
LOCUS       DAVMMR010000045        16368 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMMR010000045 DAVMMR010000000
VERSION     DAVMMR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.045"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(118..915)
                     /locus_tag="VYS74_001475"
                     /pseudo
     CDS             complement(118..915)
                     /locus_tag="VYS74_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1181..1417
                     /locus_tag="VYS74_001476"
     CDS             1181..1417
                     /locus_tag="VYS74_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV3789496.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1648..2934
                     /locus_tag="VYS74_001477"
     CDS             1648..2934
                     /locus_tag="VYS74_001477"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV3789497.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            3135..3602
                     /locus_tag="VYS74_001478"
     CDS             3135..3602
                     /locus_tag="VYS74_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV3789498.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            3635..3728
                     /gene="ffs"
                     /locus_tag="VYS74_001479"
     ncRNA           3635..3728
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYS74_001479"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3811..4952)
                     /locus_tag="VYS74_001480"
                     /pseudo
     CDS             complement(3811..4952)
                     /locus_tag="VYS74_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(5007..6077)
                     /locus_tag="VYS74_001481"
     CDS             complement(5007..6077)
                     /locus_tag="VYS74_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV3789499.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(6094..6474)
                     /locus_tag="VYS74_001482"
     CDS             complement(6094..6474)
                     /locus_tag="VYS74_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV3789500.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(6487..6750)
                     /locus_tag="VYS74_001483"
     CDS             complement(6487..6750)
                     /locus_tag="VYS74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV3789501.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(6750..6983)
                     /locus_tag="VYS74_001484"
     CDS             complement(6750..6983)
                     /locus_tag="VYS74_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789502.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(6983..7351)
                     /locus_tag="VYS74_001485"
     CDS             complement(6983..7351)
                     /locus_tag="VYS74_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789503.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            7923..8114
                     /locus_tag="VYS74_001486"
     CDS             7923..8114
                     /locus_tag="VYS74_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV3789504.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            8137..8340
                     /locus_tag="VYS74_001487"
     CDS             8137..8340
                     /locus_tag="VYS74_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789505.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(8495..8662)
                     /locus_tag="VYS74_001488"
     CDS             complement(8495..8662)
                     /locus_tag="VYS74_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV3789506.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            8682..9047
                     /locus_tag="VYS74_001489"
                     /pseudo
     CDS             8682..9047
                     /locus_tag="VYS74_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(9267..9446)
                     /locus_tag="VYS74_001490"
     CDS             complement(9267..9446)
                     /locus_tag="VYS74_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789507.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(9588..9737)
                     /locus_tag="VYS74_001491"
     CDS             complement(9588..9737)
                     /locus_tag="VYS74_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789508.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            10042..10485
                     /locus_tag="VYS74_001492"
     CDS             10042..10485
                     /locus_tag="VYS74_001492"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV3789509.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            10487..11002
                     /locus_tag="VYS74_001493"
     CDS             10487..11002
                     /locus_tag="VYS74_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3789510.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            11016..12377
                     /gene="radA"
                     /locus_tag="VYS74_001494"
     CDS             11016..12377
                     /gene="radA"
                     /locus_tag="VYS74_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV3789511.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            12450..12947
                     /locus_tag="VYS74_001495"
     CDS             12450..12947
                     /locus_tag="VYS74_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV3789512.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            12972..13755
                     /locus_tag="VYS74_001496"
                     /pseudo
     CDS             12972..13755
                     /locus_tag="VYS74_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            13900..14868
                     /locus_tag="VYS74_001497"
     CDS             13900..14868
                     /locus_tag="VYS74_001497"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3789513.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(15002..15283)
                     /locus_tag="VYS74_001498"
                     /pseudo
     CDS             complement(15002..15283)
                     /locus_tag="VYS74_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15410..15643)
                     /locus_tag="VYS74_001499"
     CDS             complement(15410..15643)
                     /locus_tag="VYS74_001499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789514.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(15636..15752)
                     /locus_tag="VYS74_001500"
                     /pseudo
     CDS             complement(15636..15752)
                     /locus_tag="VYS74_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            complement(15967..16317)
                     /locus_tag="VYS74_001501"
     CDS             complement(15967..16317)
                     /locus_tag="VYS74_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV3789515.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
BASE COUNT         4568 a         2749 c         3594 g         5457 t
ORIGIN      
        1 atatcaaaaa gaacctcaaa aaggtattac caagttgcaa taccttttac gaggctcttt
       61 tctcttgttc ttgtttcaat tgactatatt agaggcgaga catttttcgg ttctttgtca
      121 actgtagtgg gttgaagaaa gcgaagatct agaaaggaca aatttcgtcc tttctttttt
      181 gaagttttca aagttcctaa aaccaaaggc attgtgcttg ataagtttga tgagattatt
      241 ggtggcttcc agtttggcgt tggaataagg taattgaagg gcgttgacga ttttctcttt
      301 atctttgagg aaggttttaa acaaagtctg aaacagaggt ggaaaagcaa gagctgatag
      361 agattatagt ggtgtttcaa gtcttcggaa tagctcaaaa gtttatctag aatttcttta
      421 ttagtcaagt gcatacgaaa agtagggcga taaaatcgtt tatcactcag tttctgacta
      481 tcttgttgaa tgagcttcca gtagcgcttg atagccttgt attcatggga tttcggatga
      541 tggcttgtgt tctgctctca agaacagtta tgatattgag tttatcaaag tcctgagcaa
      601 taaagctcat ctccatctcc cgattgaaac agtcactccc cggactgttt caacgtccta
      661 ggacataatc tcaggaagac gcgaaaaatc atgctcaaag tgaaaattat tgatcttgcg
      721 aatgacagtt gaagttgaaa tagacaactg atgatcaatg tcggtcatag aaatcttttc
      781 aattaacttt tgagcaatct tttggttgat gatacaagga atttgatgat tcttcttgac
      841 gatagaagtc tcagcgagct ccatttttga gcaatgatag cacttaaaac ggccttttct
      901 aagaagaatt ctagtttgaa tttttttata ctagaaaatc agaaccataa tacctatata
      961 aaaatattat agttctaata ggatttaccc aaaagtttta aggcggtctt tttagaactt
     1021 taattgtttg aaatttaggt agcaaatttg tttctatttt gtcaactttt cctattttta
     1081 tcttgttgag gctggtattt taacaattca ggaattgata gtgaatgtgt aaaatttttt
     1141 gttagaataa gtttataaaa aagaaaagga gtatttgatt atgttacaaa aaatttatga
     1201 gcagatggct aatttctatg atagtattga agaagagtat ggtcctacat ttggtgataa
     1261 ttttgactgg gaacatgttc attttaaatt tttaatttat tatttagtga gatatggcat
     1321 tggttgtcat agggatttta tcgtttacca ttatcgtgtt gcttatcgtt tgtatcttga
     1381 aaaattggta atgaatcggg gttttatttc ttgttgaggt aattttagta aatttccgaa
     1441 ctaatttact cttttatgta aagataatag taaatagcta gtaatttttc taaatcattt
     1501 tttaatagtt ggaaatagca aatctttcta ttgtttcttc ttgataaaaa ggcgattttt
     1561 tattataata aattgtaaga tataattgca ggtgagagtc ctgccatgta tgtgagaaag
     1621 gaagagcctg agggctcaga caaaattatg acttcagttg ttgttgtagg tacccaatgg
     1681 ggtgatgaag gtaaagggaa gattacagac ttcctttcag cgaatgcaga agtgattgca
     1741 cgttaccaag gtggtgataa tgctggtcac acgattgtga ttgacggtaa gaaatttaag
     1801 ttgcacttga ttccatctgg gattttcttc cctgaaaaaa tatctgtcat tgggaatggt
     1861 atggttgtaa atcctaaatc tcttgtaaaa gagttgagct atcttcatga ggaaggtgta
     1921 acaactgata acttgcgtat ttctgatcgt gcgcatgtta ttttgcctta tcatatcgag
     1981 ttggatcgct tgcaagaaga agctaagggc gacaataaga ttggtacgac aattaaggga
     2041 attggtccag cttatatgga caaggctgct cgtgttggaa ttcgtattgc agatctttta
     2101 gataaagata ttttccgtga gcgtttagaa cgtaaccttg ctgaaaagaa tcgtcttttt
     2161 gaaaaattgt atgacagtaa agcgattgtt ttcgatgata tttttgaaga atattacgaa
     2221 tatggtcaac aaatcaagaa atacgtgata gatacatctg ttatcttgaa tgatgcgctt
     2281 gataatggca aacgtgtgct ttttgaaggt gcacaaggtg ttatgctaga tatcgaccaa
     2341 ggtacttatc catttgttac gtcatcaaac cctgtagctg gtggtgtgac aattggttct
     2401 ggtgtcggtc caagcaagat tgacaaggtt gtaggtgtat gtaaagctta tacgagtcgt
     2461 gtaggagatg gtcctttccc aactgagttg tttgatgaag tgggagaacg tatccgtgaa
     2521 gtgggtcatg agtatggtac aacaactggt cgtccacgtc gtgtaggttg gtttgactca
     2581 gttgtgatgc gtcatagccg tcgtgtttct ggtattacta acctttcttt gaactctatt
     2641 gatgttttga gcggtttgga tactgtgaaa atctgtgtgg cctatgatct tgacggtcaa
     2701 cgtattgact actatccagc tagtcttgag caattgaaac gttgcaagcc tatctatgaa
     2761 gagttgccag gttggtcaga agatattacc ggagttcgca atttggaaga tcttcctgag
     2821 aatgcgcgta actatgttcg tcgtgtgagt gaattggttg gcgttcgtat ttctactttc
     2881 tcagtaggtc ctggtcgtga acaaacaaat attttagaaa gtgtttggtc ctaagagatt
     2941 tttaagattt gtttaagata ggtcgggtat actatagaca gttacaagaa gacctcctaa
     3001 cttgttgtaa caaatatcct aaacttttct ttttcataat aatctcccta tagagtcacc
     3061 gcattcggtg gcttcttttg tgttgggatt catgatataa taataaaatc gataagtagg
     3121 aaaagagaaa agagatgtat tatacgcttg aagaaaaaga agtctttatg agggaggctt
     3181 tgagagaggc tgagattgct cttgaacacg atgaaattcc aattggttgt gtgattgtca
     3241 aagatgggga aatcattggt cgtgggcata atgcgcgtga ggaattacag cgagcggtta
     3301 tgcatgcgga aattatggct atagaggatg cgaacttgag tgaggagagc tggcgcttgc
     3361 tggattgcac actttttgtg accattgaac cttgtgtcat gtgtagtggg gcgattgggc
     3421 ttgcccgcat tccaaatgtg gtctatgggg ctaaaaacca gaaatttggc gctgctggga
     3481 gtttgtacga tatcttgaca gatgagcgtc tcaatcatcg tgtggaggtt gaaataggaa
     3541 ttttggaaga tgaatgcgca gctatcatgc aggatttttt tagaaataga cggaaaaaat
     3601 aattttgctt ttaaaatgaa taggaatgtg atataataaa tagtggagca acagttctgc
     3661 gtgaagcggg tcaggggagg aatccagcag ccctaagcga tttgaattgt gtgctctttt
     3721 ttcgtgcact ttaaaaaccc tttaaaatca acactttaag gggtttttgt ttgtcttgta
     3781 taagaaaaag gggcagacga ggggcacaat ttaaaatttt atcttgtcta acttgctaga
     3841 tatgtctgat accatttttt gagtaacgtg agaataaatc tctagtgtgg tctttgagtc
     3901 actatgccct actctgtcca tgatggcagt caagggaatg cccagctcag caagtaggga
     3961 tatgtgagaa tgtctaaatg tatgtgtagt tatgtttttt tctatgccga ttttttgacc
     4021 atgtcttttc aatgcacaaa taaccctggc atttgttatt ggttctccta gagtattgat
     4081 gaaaataaaa tctgtatcaa atccatttgt cgcattctct attatttgct ctttgataat
     4141 atctaacact ttttgaggtg ctgttataac cctatcggac ttgattgtct ttggtgtagt
     4201 tctctctttt tgtctgaaat cgtatgtatg cttgatgtga atagtctttt tagaaaaatc
     4261 tatatcttcc ttgtagttta aggctgccag ctctccatac ctcatgccag taagaaaaag
     4321 aactttagct attcggatat actttgtaat tcgataatca catagggcct cgtcttttaa
     4381 attttggatg aataacttaa actctttttg gtctaagtat tttgtatttt tctttctgag
     4441 ttcgtcggat gtaattactt ttctaggcgt ttcaacaaat agcatttcat ttgtatcaat
     4501 ataattcatt ctgatagcaa atttcattat ctgattgagc ttgaatttga ttttagaaac
     4561 atagttatga gatctcccat cttgtaatag ttgatctatt actttttgta ataaacgtct
     4621 atcaaatttc taactaggta gtcgccctct atctgcttta aaatctcttt ttttacattc
     4681 tttgaagcgt agacggttga gttttttacg ccgtgtttcc aattctcctc aaattcttca
     4741 tatagttttt caaaagttat attagaaaca gaatgttgtt tttctcctaa cttttgtttt
     4801 atcttttcct gaagtaaaat cgcagcttga tttcttgctt ggggagtttt tttctccatc
     4861 gttactgaga ctttttttaa tttctcagta tatggatctt tgtatctctc aaaaaattta
     4921 tatttgccgt ttggcaattc ttccatccac attgatttta cctcactttt ttgataaaat
     4981 gggtatagta aagagggctt tttttattat gagaatttct tgattacttc taaaatttca
     5041 ttggcataat ttgcgacttc gagaggggta gacaccggga agatgccttt atcacggatt
     5101 tcgatagtgc ttttttttga atttgaacga taacgtaata cccatttttt gatgttatcg
     5161 tcaaccaaaa cattgaaata gctacggtta tctctgtaaa atacacgttc tggagaaact
     5221 acatccctag caagcatttt aacaacagta taaacttcta attcagcagg agttgtgata
     5281 atttcgtcag ttacttcgac aatctcttca gcttcagctt caacttttgg aatatcggtt
     5341 gttactttgg tttcaacgct tgtattaagt gcagcactta atttttcatt aactcgctct
     5401 gtgatgaatt gattaaaccc tttcacaata atgggagaaa atgtcgttaa gatattttga
     5461 gtaacacgcc cttcatagat ttcagaagtt agatatctga ggaagctatc tgacggtgta
     5521 gtgatatttt cagtaaggaa cgctttaaga ttgttgaggt atttcaattc agaagctgat
     5581 gaaacaatat tatcaatatc aaaattctct ttgtggaatt tgatgatttc agtaaattga
     5641 ttttctttga tatcagtcac atcgattgtt aagaatggag ttgtgtccat tttatttggc
     5701 tcatctaaat cagtaaagaa tttatattct ctaccatttg tcaagatacc gaattttgat
     5761 ttagtagtta cgaaatatct gaatagttga gagtcgtgct tagtaaggtt ttctgtaatt
     5821 gatttacatt caattaggat ttgaggctcg ccatccaaaa tgatcgcata gtcgactttt
     5881 tcgccttttt taatgcccac gtcggcagta aattccggga caaattcaag tggattgaag
     5941 atatcatatc caagcgcttg gaagaacggc ataatgaagg cattctttgt ttgttcttca
     6001 ttcgtaatgc tttggctgag gtctgctaca cgtttgccga cttgttttaa atcggctttt
     6061 actttatcaa tttccatatt gatactcctt tttttaattt actaatgcta aatactcttc
     6121 tttaaccatg atttcatttg tcatggtttt tagattgtag taagacatga atttgaggta
     6181 atcaaactct gtggggtcgt ctaagttttc tagagcgtct tttacgagat gatggatcat
     6241 attcctatca gcttcgtttt cacagcgtag acgggcgttc tggtgctctg agcgtgtgtg
     6301 gtctttgtgt ccaagttcgt gtagggcgac ttggatttgt tgttcaggag tcaagttgtg
     6361 gtcaatagcg agcacgtttg tgtctggatt gtagaaaccg ctattgtgcc agtttgaacc
     6421 gtcgaagaga caaagttcta cttgatattc ttcgcagagt ttagcgagtg tcatagttct
     6481 ccttcgttat ttatagacat ctctgcggtg ggcgatttcc acggctagga cgactagttt
     6541 atcgtcttgg atatcacaga tgatgcggta attctctact ctgtatcgcc agtaacctgc
     6601 aaggttggct tttagtgctt ttccgtgttg tcgtggattg gtcgtgtttt caatgttttt
     6661 agcaagccag gataggattt gttttctggt tggggtatct agttttttaa gttgcttgag
     6721 agctttttta tcaatatcta gccgatacat taagcaatat cctctcgagt tagtcctagt
     6781 tcatccaaga cctcatccat ggtataagta gctgggtcgg ctaaatactc catataggct
     6841 tggtcggctg ctcgtgcgtc ttcgatatct tccatgagtg ccatgaagtc gtcaaaatcc
     6901 atggtcgttg tgtcgatacc gtgtttgttt aggtagtccg taatgtagga atttttttct
     6961 gtgaagttga tagtgatagt cattagcgtt ctcctttgct tttgaagtgg gcggatagga
     7021 cggatgtaat gaagtcgata tcatcttcat ttagtggttt cccgtcaaat aacatggtgt
     7081 tggctgctgc tttgcgtaag tctatgattt gtccgtttac ttgggcaaat tcatcactcc
     7141 cagcgatagc agggttatct gttcgtccga gcaggtagtc ggtggatacg ttgaaataat
     7201 tagcaatttc tgcgatacgt tcagcgtttg gcgtagaatt ttttatctta tagagtgtat
     7261 ttctaccata acctaagtct tcttctactt gtccaagagc ttttccacgc ttttttgcca
     7321 attcttttat tttttcaaat gtctcaaaca ttgttaaatc aacctttcta agacatcaca
     7381 aaaaaaattt aacaaatttg gtgtaaaaag gttgactaat tatcccaaaa ggtgtaaaat
     7441 gttttttgta agtgataaac aactaaaaaa acaactaaga aataaattat aaaaatgttt
     7501 tggcgaacgg tatttataga tttattggtg tttttattat gctttaattt tagccgattt
     7561 ggtgtaagtt gtcaagtata atgcagaaaa atagttacat ttttagttgt ttcttattta
     7621 caaataagta aagaggagga acgtgctgat ggtaagtatt cttaaaaatt tagaacaaga
     7681 aaaagaccac cttgaaaaag tcattaaggt agtcagcgct ggtggtaaat ttctgagatt
     7741 gccatatcaa aaaagtcacg ctcgattagt gagaatctga aattgatttc tcaaaatctt
     7801 taccatcatc gtagatgtcg cgtttgcttg ctttgagtaa atattcttta aaagtcataa
     7861 ggttaactcc tttctgctta tattatagca gaaaaagaag aaagaaaaag aaaggagaaa
     7921 atatgccaga tatcgcaaac ggtcgcgaaa gagttattgc tttcttgaaa gagaaaggca
     7981 ttaaaaaagc aactctagcg gttgcttacg gctttaaacg acaggaagtg acaaacattc
     8041 taagtggaac gactaaaggt ccacgagcga acagtttcat tcttcaggtt attgaagatt
     8101 atgggattga gtaggaaaga tttgaggagt aggaaaatga gaccaaaacg atatccgtat
     8161 agtggacaaa aagagtccac ctttgtaaag gcagaccctg agttagttga aaaactttta
     8221 agaaacacta gttttcttga gtgtttacaa aaaaagccta tcaattttca gatagactca
     8281 gaagaattta agcgtcttag ctatgaagcc attcatgata cttctcaagt aactcaatag
     8341 gaggaaggaa taaaatgatt caccattata taactcacta tgccagcaat gggaaagatt
     8401 acgccgaagc atggattcaa attgattttt tgggaatgtg cttttgtgta tggaaaaagc
     8461 gtacaaccat tgaacgattg tacgcaaacg aagattagac ttttttccaa ccgttgcctt
     8521 tagcagatgt cggagggagc cgatcacctt ttccgatagt tgcggtatga ccattagtaa
     8581 cttttccgcc acgaggtcct acctctacat agcgaccagg tttctgatta tctgttccag
     8641 gttttattgg agtatttgcc atactatctc ctcctttcta taagtttgag aaaatcaacg
     8701 gtatctggta ttatttcgat ggctcaggct atatgctttc agaccgctgg aagaagcaca
     8761 cagacggtaa ttggtactac tttgaccaat caggcgaaat ggccacaggc tggaagaaaa
     8821 tcgctgacaa gtggtactat tttgatgtag aaggtgccat gaagacaggc tgggtcaagt
     8881 acaaggacac ttggtactac ttagacgcta aagaaggcgc catggtatca aatgccttta
     8941 tccagtcagc ggacggaaca ggctggtact acctcaaacc agacggaaca ctggcagaca
     9001 agccagagtt cacagtagag ccagatggct tgattacagt taaataaata gaaaggaaac
     9061 tttctaaatt gttctttcac cgcaggctca ggcttgcggt tttttatttg ctctgattct
     9121 tcaaaaaagc gttttcttga agaataggga ggaggaatgg cagggtatta ttgccaaaaa
     9181 cgccattttg ttaataatag ctccttttta ttttttgatt attgtcaaaa acggtgtttt
     9241 gtcaaaaata aaaacagtga ccgaaatcac tgcttatcag ttgtagcaaa ttcataaagt
     9301 ttttctgctg tgagaagtgc cattttgtcc atgcttgttt ttccttttct gaggtcagaa
     9361 acagtagtcc atggaactcc agcaccttgg gaaatagcag atgtagacat cgaactgtct
     9421 aacaattctt gaataacttc tctcatctta tttgtccttt ttatttttta gataaatata
     9481 tacattgacc gcaattataa aaatagctat tgcactaacc attgcttttc ctcttttcat
     9541 ttgataaaat agaggtgtga ggggctttcg ccccaacctc ttagcgttca cctttttctt
     9601 tgacgggatt tcggtttacg ctttttgttt tgccttgcga ccgttattgc agtcaccaga
     9661 cttgctatag cagttaccgt ttcagggata ttatctatcg ccttttccag taacctaagc
     9721 caatcttctt tgttcaactt cctcacctcc tttccttatc ttgattatat tatatcacgg
     9781 taaaccgtga aagtcaagcg atttgatgaa gtttttttga aaaacaaagg ggcaaaaaag
     9841 gggcaaaagt gtcgtaaacc tctgtaaaac gatgtaaaaa atcaactttg ccctcgcttt
     9901 aaagctctaa atttcaacgt attgtgaaac agtgtaaatt atcgtatcgc ctataattgt
     9961 tgtgtgctct ttttttcgtg ctttttccga ataaataaga tagaataatc tagaataaat
    10021 gataatagaa aagagaaaat tatgaaaatt cgtggttttg aattggtttc gagttttaca
    10081 gatgaaaatt tattgcccaa gcgtgagaca gcgcatgcgg ctggttacga cttaaaggtt
    10141 gctgtgcgta cagttgttgc gccaggagag attgtcttgg ttccgacagg ggttaaggct
    10201 tatatgcagc cgactgaggt tctctacctc tatgatcgtt cttcaaatcc tcgtaagaag
    10261 ggcttggttt taattaactc agttggggtc attgatgggg attattatgg aaatcctgga
    10321 aatgaagggc atatttttgc gcagatgaag aatatcacag atcaagaggt tgttcttgaa
    10381 gttggggaac gtattgtcca ggctgttttt gctactttct taattgcaga tggagatgca
    10441 gctgatggcg ttcgaactgg tggatttgga tcgacagggc actagaatga agattatctt
    10501 tgtacgtcat ggggagccag attaccgtga gttagaggag cgttcttata taggatttgg
    10561 gatagatttg gcacccttgt ctgagatggg acggcagcaa gtccagaaat tgagcaaaaa
    10621 tcctttactc tcgtcagctg aaataatcgt atcttctgca gtcacaagag ctttagaaac
    10681 ggcttcgtat gtggtctgtg ctacgggtct tcctttaaga gtagagcctt tattacatga
    10741 atggcaggtc tataaaacag gaatagaaaa ctttgaaaca gctagaagac tgtttttaga
    10801 aaacaagggg gagttgcttc ctaatagtcc tattcaatat gagacagcta cggaaatgaa
    10861 gtctcggttt ctagaatgta tgtctaagta tcgagaacat cagactgtgg tagttgttgc
    10921 tcatcgaatg ctcatgcgcc agtttgtgcc aaatgagaag attgattttt gccaagtgat
    10981 tgagtgtgag ttagagatat agaaagaggt ttgtcatcgc aaagaaaaaa gcgacatttg
    11041 tatgtcaaaa ttgtgggtat aattccccta aatatctggg acgttgcccc aactgtgggt
    11101 cttggtcttc ttttgtggaa gaggttgagg ttgccgaggt caagaatgcg cgtgtgtcct
    11161 tgacaggtga gaaaaccaag cccatgaaac tagctgaggt gacttccatc aatgtcaatc
    11221 gaaccaagac ggagatggag gaattcaacc gtgtgcttgg aggcggagtg gtaccaggaa
    11281 gtctcgtcct catcggtggg gatcctggga ttgggaaatc aactcttctc ctacaagtct
    11341 caacccagtt gtcccaagtg gggacagttc tctatgtcag tggggaggag tctgcccagc
    11401 agattaaact acgtgcagag cgcttaggtg atattgatag tgagttttat ctctatgcag
    11461 agaccaatat gcagagtgtt cgtgcagaag tggagcgtat ccagccagac tttctcatta
    11521 ttgattccat ccagaccatc atgtctcctg agatttcagg ggtgcagggg tctgtttctc
    11581 aggtacgtga agtgaccgct gaactcatgc agttggccaa gaccaataac attgccatct
    11641 ttatcgtagg tcatgtgacc aaagaaggaa ccttggctgg gcctcgtatg ttggagcata
    11701 tggtggatac ggtgctttac tttgaagggg agcgtcacca cacctttcgt attttgagag
    11761 cggtcaaaaa tcgttttggt tccactaatg agattgggat ttttgagatg cagtcgggcg
    11821 gcttggttga ggtactcaat ccgagtcaag ttttcctaga agagcgtttg gatggggcga
    11881 ctggttcctc catcgttgta accatggaag ggacgcgtcc gattttggcg gaggttcagg
    11941 ctttggtaac accgaccatg tttggaaatg ccaagcgtac tacgacagga cttgatttta
    12001 accgtgctag cttgattatg gctgttttgg aaaaacgggc agggcttctc ttgcaaaatc
    12061 aggatgccta tctcaaatct gctggtggtg ttaaattgga tgaacctgcg attgacttgg
    12121 ctgttgcagt tgctattgct tcgagctaca aagacaagcc aactaatcct caggaatgtt
    12181 ttgtcggaga actgggcttg acaggagaga ttcggcgcgt gaatcgtatt gagcaacgca
    12241 tcaacgaagc tgctaaactg ggctttacta agatttatgt acctaagaat tccttgacag
    12301 gaatcactct gcctaaggaa attcaggtca ttggcgtgac aacgattcag gaagtcttga
    12361 aaaaggtctt tgcataatcc gtgacaaatt ctcttaaaaa tgataagata ggagaaatat
    12421 ttgactatca aattttcaag gagggaatcg tgtcgtattt tgaacagttt atgcaagcca
    12481 atcaggctta tgttgcccta catgggcagt taaatctgcc acttaaacct aaaaccagag
    12541 tagccattgt gacctgtatg gactcacgtt tgcacgttgc gcaagctcta ggtttggcac
    12601 ttggggatgc tcatattttg cggaatgcag gtggtcgagt gactgaggat atgattcgtt
    12661 ctctagttat ttcccagcaa caaatgggga caagagaaat tgtggtattg catcatacag
    12721 actgtggtgc tcagaccttt gaaaatgaac cttttcagga gtatttgaag gaagaattgg
    12781 gtgtcgatgt gtcagaccag gacttcttgc ccttccaaga tatagaagag agtgtacgcg
    12841 aggatatgca actgcttatc gagtctcccc taataccaga cgatgtcatt atctctggtg
    12901 ccatttacaa tgttgataca ggaagtatgg cagtcgtaga attataaata cttcatttag
    12961 aaagaaagtg tatgaagaaa agcagtattt tatttatttt tattttattg ctatgtattg
    13021 gtttacagta tgaaaccatc tactatacgg acggttcgag gtcaggtgcg gaatatggac
    13081 taatgggagt ttctatcttt ctagctctct tttacatgat tccggctctt tattttctct
    13141 tccgtattgg gaaaaaatgg gaattgccaa agaaggtttt gattctgtct ttattgggag
    13201 ggatgttcct tttaggctgg ttgtctagtt ttgctaatac ttatatccat gatttactgg
    13261 gggttctttt cccagatagt ccatttttaa atgcctttga aagtgctatt gtggctcctt
    13321 tggtagaaga acccttgaaa ttattgccac ttgtttttgt tttggctttg attcctgtgc
    13381 gaaaattaaa atttttgttt ttacttggaa ttgcctccgg tttgggattc caaatgattg
    13441 aggatattgg ttatattcgt acggatttgc cagagggctt tgactttact atttcgcgaa
    13501 ttttagagcg tatcatctca ggaattgcct ctcactggac tttttcaggt ctagctgtag
    13561 taggtgttta cttgctttac agagcctata aaggacagaa ggttggcaag aaacagggtc
    13621 ttattgttta actctccttt tgtggagttg gaaacagagt tgcctttagc gattccagtg
    13681 gttacggcta ttgctctcta tggttattat catgcttatt gctttgttga gaaacacaat
    13741 gagttgatga cctagaatat ttttcaaaag aatgatgcaa gggtacaaat atggtgccct
    13801 tcttttattt ttgattgaaa aatagtgcaa aaagcgctac aatggtagat ggaaaatctt
    13861 gtgaaaagca caagcgatac atatataccg gaggaaatca tgtctttttc tgatttaaag
    13921 ctgtttgccc tttcttctaa taaagaattg gcagaacgtg tggcgcagga gattgggata
    13981 gagttgggga aatcaagtgt tcgccaattt tcagatggag agattcaggt caacattgaa
    14041 gaatcaatcc gtgggaaaca cgtctttatc ttacaatcaa ctagttcgcc tgtaaatgac
    14101 aatctgcttg aaattttgat tatggtagat gctttgaagc gtgcgagtgc agagtctgtc
    14161 aatgttgtca tgccttacta tgggtatgca cgtcaggata gaaaggcgag agcgcgtgag
    14221 ccaatcactt caaaacttgt cgcaaatatg cttgaagtag ctggagtgga tcgtttattg
    14281 accatcgact tgcatgctgc gcaaattcaa ggattctttg atattcctgt ggatcatttg
    14341 atgggtgctc ctctaattgc agattatttt gagcggcgtg gtatggttgg ttctgactat
    14401 gtggttgtca gcccggacca tggaggggtg actcgtgctc gtaagttggc agaatttttg
    14461 aaaacatcta tcgctattat tgataaacgt cgtagcgttg ataagatgaa tactagtgaa
    14521 gttatgaaca tcatcggtaa ggttgaaggc aagacttgta tcttgattga tgatatgatt
    14581 gataccgctg gaacgatttg tcatgcggca gatgctcttg cggaagctgg tgctgttgca
    14641 gtctatgcaa gctgtacgca cccagttctt tctggtcctg ctacggacaa tatccaaaaa
    14701 tcagctatta agaaattggt tgttttggat accatctatc tgccagaaga gcgtttgatt
    14761 gataagattg agcagatttc aatcgctcat ctcctagggg atgctatcgt acgtattcat
    14821 gaaaaacgac cactttctcc acttttcgat attgagaaaa aaatttaatg accaagcctg
    14881 agatgattct caggtttttt cgtctttttt ccgaataaat ggatagtagc ggtgaatcta
    14941 gtaaagctag atttaaaact gtgatataat aaaaagagaa gaaatatgac tatacgtcat
    15001 cagaagtttc agcgaccatc atttttgaac agtgatagca cttgaatcga cgctttctaa
    15061 ggagaattct agtaggtata ccagtcgttt caagataagg aattttagaa ggtttttgaa
    15121 agtcatattt cttcaattgg tttccgcact cagggcaaga tggggcgtcg tagtccagtt
    15181 tggcgatgat ttccttgtgt gtatccttat tgatgatgtt taaaatctgg acattagggt
    15241 ctttaatgtc tagtaatttt gtgataaaat gtaattgttc catatgattc tttctaatga
    15301 gttgttttgt cgcttttcat tataggtcat atgggacttt ttttctacac aaaataggct
    15361 ccataatatc cataggggat ttacccacta tggatattat ggagccattt tacaacagtg
    15421 cctcaaattc agatactgac atacctaatt gctggctggc aacctcagaa gtcagaagac
    15481 cttggcgtac tatgtctagg aaggcaaaaa gtttcctttc tgcccttcct tcaactttcc
    15541 cacgctctag tccttgttca aggccttcag ccctagcagt ttccaaggca tagtcctgtg
    15601 ctaacaagac ttgttcttct cgcatatgta gttgactaaa cattttcctg tcctcctcgg
    15661 accagctctt gtagtccagc agttgatttg cttggctaat ggctcgttgc ggttgctggg
    15721 taaagggctt gttgccgaaa aactccaacc atggcttgcg aaccttgtct ttgctggttt
    15781 ctctgtattt tttaattcca agaatgccat cttaaccaga tggttttcct gtccattgtt
    15841 ggtaatcgcc aatacctcac ctgttgtgtc ttcgcgcata ctaaagctat gaaaagccag
    15901 gtcatctgag aaataattac tatccacgat agcaatggcg taaacaggag cgatgtgttt
    15961 gtagctctag tgagtatcac cttctcgctg acgaattttt tcaagatttt gattaacctg
    16021 actgcacagg taagtccaca agtgattgat gaaaaaattc tgatgatgga cttgaatctc
    16081 aataattact tgagtaccgt tatccaactc cgccaagacg tctatactgg tataaaaatc
    16141 ctgcaccgag taaggcatgg agagtaatac gtgaatatcg cttccctcca aaatggtcac
    16201 attttttgct ggcaagtcca gcatatcgcg aataaattga caagtgattt ctggattgct
    16261 gaatattttc ttagcaatca aatcattagt tgggctgatg cccggatgtc tgagaatcct
    16321 cctattcctc cttctatagt gagttgaaat aagatgtgaa taactcta
//
LOCUS       DAVMMR010000046        16050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMMR010000046 DAVMMR010000000
VERSION     DAVMMR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.046"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            216..4058
                     /gene="spuA"
                     /locus_tag="VYS74_001502"
     CDS             216..4058
                     /gene="spuA"
                     /locus_tag="VYS74_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV3789516.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDINTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKENISATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(4158..5207)
                     /locus_tag="VYS74_001503"
     CDS             complement(4158..5207)
                     /locus_tag="VYS74_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV3789517.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5326..7134)
                     /gene="glmS"
                     /locus_tag="VYS74_001504"
     CDS             complement(5326..7134)
                     /gene="glmS"
                     /locus_tag="VYS74_001504"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV3789518.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7326..8705)
                     /gene="bglA"
                     /locus_tag="VYS74_001505"
     CDS             complement(7326..8705)
                     /gene="bglA"
                     /locus_tag="VYS74_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV3789519.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8805..10658)
                     /locus_tag="VYS74_001506"
     CDS             complement(8805..10658)
                     /locus_tag="VYS74_001506"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV3789520.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10671..11930)
                     /gene="rseP"
                     /locus_tag="VYS74_001507"
     CDS             complement(10671..11930)
                     /gene="rseP"
                     /locus_tag="VYS74_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV3789521.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11952..12755)
                     /locus_tag="VYS74_001508"
     CDS             complement(11952..12755)
                     /locus_tag="VYS74_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV3789522.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12764..13522)
                     /locus_tag="VYS74_001509"
     CDS             complement(12764..13522)
                     /locus_tag="VYS74_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV3789523.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13753..14217)
                     /locus_tag="VYS74_001510"
     CDS             complement(13753..14217)
                     /locus_tag="VYS74_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV3789524.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14306..15277)
                     /gene="ruvB"
                     /locus_tag="VYS74_001511"
     CDS             complement(14306..15277)
                     /gene="ruvB"
                     /locus_tag="VYS74_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV3789525.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15438..15530
                     /locus_tag="VYS74_001512"
     CDS             15438..15530
                     /locus_tag="VYS74_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV3789526.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         4867 a         3775 c         3045 g         4363 t
ORIGIN      
        1 aatttctgaa taaaaatagc tggtaacaca tcatctccct aataatacgg cttggcttat
       61 gtgacatttt tatctccgtc atttatactg atatataaaa tagtacagaa aacttctcaa
      121 aagtaggaat ttttttcaca aatcaaagaa aacgcttgca tattgtttta ctacatgcta
      181 tactgatata gaaatttatt gattggagaa tcattatgag aaaaacacca tctcacactg
      241 agaaaaaaat ggtctacagc atccgttccc tcaaaaatgg aactggttct gtccttatcg
      301 gcgcaagcct tgtcttgctt gccatggcta caccaactat ctcatccgac gaaagtacac
      361 caaccactaa cgaacccaac aacagaaata caaccaccct tgcccaacct cttactgata
      421 cagcagctgg ctctggtaag aacgaaagtg atatttcttc acctagaaat gcaaacgctt
      481 ccctagagaa aacagaagaa aaacctgctg caagcccagc cgatccagca ccacaaactg
      541 gacaagatcg ttcaagtgag ccaactactt ctactagtcc agtaacaact gaaactaagg
      601 cagaagagcc catcgaagat aactacttcc gtatccatgt caaaaaactt cctgaagaaa
      661 acaaggatgc tcaaggacta tggacttggg acgatgttga aaaaccatct gaaaactggc
      721 caaacggagc tttgtccttc aaggatgcca agaaagatga ctacggctat tacctagatg
      781 tcaaattaaa gggagaacaa gccaagaaaa ttagcttcct catcaacaat acagctggaa
      841 aaaatctaac cggcgataaa tctgtagaaa aactagttcc aaaaatgaac gaagcttggt
      901 tagaccaaga ttacaaggtt ttctcttacg agccacagcc tgcaggaact gttcgcgtca
      961 actactaccg cacagatggc aactatgaca agaaatctct ctggtactgg ggagatgtga
     1021 aaaatccaag tagcgctcaa tggcctgacg gaacagactt tacggctaca ggcaaatatg
     1081 gccgctatat cgacattcct cttaatgaag ccgcaagaga atttggattt ttattactag
     1141 atgagagcaa acaaggagac gacgtgaaaa tccgtaaaga aaattataag ttcacagatt
     1201 tgaaaaatca tagccaaatt ttcctaaaag acgatgatga atcgatttac acaaatccat
     1261 actatgtcca tgatatccgt atgacaggag cccaacacgt aggcacttct agcattgaaa
     1321 gtagcttttc aacacttgtc ggtgctaaaa aagaagatat cctcaaacac tctaacatca
     1381 ctaatcacct aggaaacaag gtaactatta ccgatgttgc aatcgatgaa gctggtaaga
     1441 aagtgaccta cagcggagat ttctctgaca caaaacatcc ttatactgtt agctacaatt
     1501 ccgaccaatt cactaccaaa acaagctggc gcctgaaaga tgagacatac agctatgatg
     1561 gcaaactggg agctgaccta aaagaagaag gaaaacaagt tgatttgacc ctttggtcac
     1621 caagtgctga taaggtttct gttgttgtct acgacaagaa tgaccctgac aaagtagttg
     1681 gaactgtcgc tctcgaaaaa ggggaaagag gaacttggaa acaaactcta gacagcacaa
     1741 acaaactcgg aatcacagat ttcactggct actattatca ataccaaatc gagcgtcaag
     1801 gtaaaactgt tcttgcactc gatccttacg ctaaatctct tgctgcttgg aatagcgacg
     1861 atgccaagat tgacgatgcc cataaagtgg ctaaagccgc ctttgtagat ccagctaaac
     1921 tcggacctca agacttgact tatggtaaga ttcacaattt caagactcgt gaagacgccg
     1981 ttatctacga agctcatgtg cgtgacttca cttcagatcc tgccattgca aaagacttga
     2041 ccaaaccatt tgggactttt gaagccttca ttgaaaaact agactatctc aaagacttgg
     2101 gtgtaaccca tatccagctc cttccagtct tgtcttacta ctttgtcaat gaattgaaaa
     2161 accatgaacg cttgtctgac tatgcttcaa gcaacagcaa ctacaactgg ggatatgacc
     2221 ctcaaaacta cttctccttg actggtatgt actcaagcga tcctaagaat ccagaaaaac
     2281 gaatcgcaga atttaaaaac ctcatcaacg aaatccacaa acgtggtatg ggagctatcc
     2341 tagatgtcgt ttataaccac acagccaaag tcgatatctt tgaagaccta gagccaaact
     2401 actaccactt tatggatgcc gatggcacac ctcgaactag ctttggtggt ggacgtttgg
     2461 ggacaaccca ccatatgacc aaacggctcc tagttgactc tatcaaatat ctagttgata
     2521 cctacaaagt ggatggcttc cgcttcgata tgatgggaga ccatgatgcg gcttctatcg
     2581 aagaagctta caaggctgca cgcgccctca atccaaacct catcatgctg ggtgaaggtt
     2641 ggagaaccta tgctggtgat gaaaatatgc ctactaaggc tgctgaccaa gattggatga
     2701 aacataccga tactgttgct gtcttctcag atgacatccg taacaacctc aagtctggtt
     2761 atccaaacga aggtcaacct gcctttatca caggtggcaa gcgtgatatc aacactatct
     2821 tcaaaaacct cattgctcaa ccaaccaact ttgaagcaga cagtcctgga gatgtcatcc
     2881 aatacatcgc agcccatgat aacttgaccc tctttgacat cattgcccag tctatcaaaa
     2941 aagacccaag caaagctgag aactatgctg aaatccaccg tcgtttacga cttggaaatc
     3001 tcatggtctt gacagctcaa ggaactccgt ttatccactc tggccaggaa tatggacgta
     3061 ccaaacaatt ccgtgaccca gcctacagaa ctcctgtagc agaggataag gttccaaaca
     3121 aatctcactt gttgcgtgat aaggacggca acccatttga ctatccttac ttcatccatg
     3181 actcttacga ttctagtgat gcagtcaaca agtttgactg gactaaggct acagatggta
     3241 aagcttatcc tgaaaatgtc aagagccgtg actatatgaa aggtttgatt gcccttcgtc
     3301 aatctacaga tgccttccga cttaagagtc ttcaagatat caaagaccgt gtccacctca
     3361 tcactgtccc aggccaaaat ggtgtggaaa aagaggatgt agtgattggc tatcaaatca
     3421 ctgctccaaa cggcgatatc tacgcagtct ttgtcaatgc ggatgaaaaa gctcgcgaat
     3481 ttaatttggg aactgccttt gcacatctaa gaaatgcgga agttttggca gatgaaaacc
     3541 aagcaggatc agtcggaatt gccaacccga aaggacttga atggactgaa aaaggcttga
     3601 aattgaatgc ccttacagct actgttcttc gagtctctca aaatggaact agccatgagt
     3661 caactgcaga agagaaacca gactcaaccc cttccaagcc tgaacatcaa aatgaagctt
     3721 ctcaccctgc acatcaagac ccagctccag aagctagacc tgattctact aaaccagatg
     3781 ccaaagtagc tgatgcggaa aataaaccta gccaagctac agctgattca caagctgaac
     3841 aaccagcaca agaagcacaa gcatcatctg taaaagaagc ggttcgaaac gaatcggtag
     3901 aaaactctag caaggaaaat atatctgcaa ccccagatag acaagctgaa cttccaaata
     3961 caggaatcaa aaacgaaaac aaactcctat ttgcaggaat cagcctcctt gcgctccttg
     4021 gtctcggttt cttactaaaa aataaaaaag agaactaagc tagccctcct atagaaaaat
     4081 cccccaagca ttatagctcg ggggattaat tttcgtacaa tatttgttgt cctaataaac
     4141 ttgattagga ttttttatta agcctctttc atagcaaaat aagctcgtac tttgggtgca
     4201 acttgtgttc cgaagagttc aatagctctc agaacctggt catgaggcat agaaccaagc
     4261 ggtagatgaa gcatgaagcg gtccaatcct aaatcctcta tcatgcgaat caatttttcg
     4321 gccacctgat ctggattgcc aacaaacatg gcgccatttg accctacctg ctccaaatat
     4381 tgctcataac gcaattcctg ccagtgcgga cggtctttgg aaatagcatc caccacttgc
     4441 ttagtcggat ggaaataatc tttcaccgcc tgctcaccat cttccgcaat ccacccccaa
     4501 gaatgggctc ccactttcaa gtctttgtca gcatggcccg cttcgcttcc aatctcacga
     4561 taagcctgaa tcaacttttt aaaataacgt ggattaccac caataatagc atatacaatc
     4621 ggtagaccag cctgagcaat cttcactgtt gattcgacat gaccacctgt agctatccac
     4681 aagggcaatt tgtcctgaac tggacgagga taaacttctt taccagcaat cgtttgagtc
     4741 aatcgacctt gccagtctaa cttggtcttt tcattgacta actgaagcaa gtctaatttc
     4801 tcatcaaaaa gagagtcgta gtctttcaag tcataaccaa acagagggaa agattccgtg
     4861 aaagagcccc ttccagccat aatctccgat cgtccatttg acaaagcatc gatagtggca
     4921 tactgttgga acaaacgaat cgggtccatg cttgacagaa tgctgactgc actggtcaaa
     4981 cggattttct tggtgttgac tgccccagcg gccaggacaa tctctggggc tgatactgca
     5041 aaatccgccc gatggtgctc gccaatccca tatacgtcca aaccaacctt gtcagccagc
     5101 tcaatctctg ccaccaactg gcgaatgcgt tcagcatgac tgtaagtttg tccagtccct
     5161 tcaagctccg ttatttcccc aaatgttgaa attcccaatt ctaccattgt gattctcctt
     5221 gtctatctct gtccttcaat ttgaaaaatt attctaacac gaatcttaag tacaagcaac
     5281 caatttgctc attagaaaaa gcctagataa ctagactttt ttagcttatt ctaccgttac
     5341 tgatttagca aggttacgtg gtttatccac atcgaggcca cggtggaggg ttgcaaagta
     5401 agcgactaat tgcgttggta cgaccattga aattggtgag aggtatggat gtacggtcgt
     5461 aaggacgata tcgtcggtat ctttggctac attctcttct gcgatagtga ggactttggc
     5521 accacgggct gcgacctctt ggatatttcc acgagtatgg ttggcaagaa ctggatctga
     5581 caagagagcc aaaacaggcg ttccttcttc aatcaaggca atggttccgt gcttgagttc
     5641 tcctgccgca aaaccttcac actggatata agaaatctct ttgagtttga gacttgcttc
     5701 catggctacg tagtaatctt gaccacgtcc gatgtaaaag gcgttacgag ttgtttcaag
     5761 aagttcacga accttggctt caatggtttc tttctctgaa agagttgatt cgatagactg
     5821 agctacgatt gacaattcat gaaccaggtc aaaggcttgc gctttagcat taccatttgc
     5881 ttctccgact gcttttgcaa ggaaggcaag ggctgcgatt tgcgctgtat aggctttagt
     5941 tgatgccacg gcaatttcag gacctgcgtg aaggagcatg gtatagttgg cttcacgtga
     6001 gagggttgaa cctggaacgt ttgtcactgt taagcttgga attcccattt cattagcctt
     6061 gaccaaaact tgacgactat ccgctgtttc accagattgg ctgataaaga tgaagagtgg
     6121 tttcttgctg agaagtggca tactgtagcc ccactcagat gagattccaa gttcaactgg
     6181 tgtatctgtc aattcttcca acattttctt agaagcaaat cctgcatggt aagatgttcc
     6241 agctgcaagg atgtagatgc ggtctgcgtc ttgaacagcc ttaatgatat ctgggtccac
     6301 gacaacttga ccagcctcat ctgtgtaggc ttggatgagt ttccgcataa cagttggttg
     6361 ctcgtcaatt tccttgagca tgtagtaagg gtaagttccc ttaccgatat ctgacaagtc
     6421 aagctcagca gtgtagctag cacgctcacg acgatttcca tcatagtctt gaacttccac
     6481 actatcagcc ttgacgatta ccaactcttg gtcatggatt tccatgtatt ggttagtttc
     6541 acgaatcata gccatggcgt ctgagcagac catgttatag ccttctccaa gaccaatcaa
     6601 aagtggtgat ttatttttag ctacgtagat gacttcagga tcttgtgagt caaccaaggc
     6661 aaaggcataa gaaccacgga tgatgtgaag ggcttttttg aaggcttcaa gaactgagag
     6721 cccttcttct tccgcaaatt ttccaatcaa atgaacggct atttcagtat ctgtctgccc
     6781 cttgaagtgg tgacctgcaa ggtattcttc cttgatttca agatagttct caatcacccc
     6841 attatgcacc aagacaaaac gttctgtctc agagcggtgt gggtgagcat tgtcctcagt
     6901 tggttttccg tgagtagccc aacgagtatg tccgatacca gttgttccct caacaccagc
     6961 tgtcttggca gacaattctg caatacgacc aaccgccttc accaaatggt tatcagcacc
     7021 atctaggaca aaaattcccg cagaatcata gccacggtat tcaagctttt caagcccttg
     7081 aatcaaaata tcagttgcat ttgtgtttcc aacaacacca acaattccac acatagtata
     7141 tacgacacag gcaagctgtg ctttctcctt aaaattggta tagtctaatt catcttttat
     7201 agaatcagca aaaacagtat atacttgttt ctttcacttg tcaagagtaa aaattggtat
     7261 agttcaaatt aagctcctgt aagcaaaaaa ctctgaccga ttgggataat cagtcagagt
     7321 cctttttaaa atccattatt atcgcttaat tctttgaacc agtggcctga tttcttcaga
     7381 cgacgttctt gcgtttccaa gtctaattcg accaaaccat agcgattttt atagctgttg
     7441 agccatgacc agcagtcaat aaaggtccaa atcaagtagc ccttacagtt ggcaccatct
     7501 tcaatggcac ggtgaagttc acgaagatga ccttttacaa agtcaatacg gtaatcatct
     7561 tgaatcattc catcttgacg gaatttttct tccccttcaa cacccatacc attctcagtc
     7621 aacatccact caatattgcc ataattttcc ttgatatttt gggcgatgtc ataaatccct
     7681 tgctcataaa tctcccaacc acggtgagaa ttgattttac gtccaggcat cacataaggc
     7741 tcgtaaaaat gttctggtaa gagtggacta tctggatgct tagcaaatcg aggagccata
     7801 acacgcaaag gttgatagta gttcacacca aggaagtcca ccgtattatc acgaatgagt
     7861 tccaactctt cctctgtagc atcaggtaaa agaccgtgtt catgcaagat ttctaccaac
     7921 tcctgtggat aagtccccaa gacagatgga tctaagaaag attgggcctg aaaaagggcc
     7981 gcaatacgag ctgccttgac atcagcagga tgctggctac gtggataagc cggtgtcaag
     8041 ttgaggacaa tcccaatctt ggaatcaggc aaaagttcat ggcaagcctt aacagcccgg
     8101 ctgctggcca attgtgtatg ataggctacc ttaacagctg cctctgcatc caccttatgt
     8161 ggataatggg catcataaaa ataaccaaat tctacaggaa cgatgggctc gttaaaggta
     8221 atccattgat ccactaaatc tccataagtc tcaaaacaaa aacgagcata gtcttcatag
     8281 gctgagactg tcgccttatt ttcccaacca tcaccatcct cttgaagggc aaaaggtaaa
     8341 tcaaaatgat agagattgac taacagacga attcctttag ccttaatagc ctcaaagacc
     8401 ttacgataaa aatccacacc ttgagggttg acttttccac agccttgtgg aaaaatccgt
     8461 gaccactgaa tagaagtccg aaagactgtg tgaccagtct ctaacaaaag ctcaatatcc
     8521 cgctcccaat tttcataaaa agtcgatgtc ttatctgaac caatcccatt atagtaacga
     8581 tttggctcca cttggaacca gtaatcccag agattgtctc ccttaccgtc accagctaca
     8641 cgtccttctg tctgcggtcc agaagtagag gatccccaga caaaatcctt tggaaatctt
     8701 agcataacat ttacctcttt atctactcat ttctcccatt atacagaaaa aacaaggtaa
     8761 aaactagtta cattttttcc ttgtttttct tctgattata gtttttattt cttgcttagg
     8821 atttcaagcg tttcaagcac gttatctgca tgaacctcaa tggtgtcacc agttgccttg
     8881 atcttaactt ctacaatgcc atcggccgct tttttaccaa cagtgatacg gattggaaga
     8941 ccaatcaagt cactatcgct aaatttaaca ccgacacgtt cgttacggtc atctgtcaag
     9001 acttcataac cagctcccat caagcttgct tcaagttttt ctgtcaaggc ttgcgcttct
     9061 tcatccttga cattgacagt aatcaaatgc acatcaaatg gtgccaattc tttagggaaa
     9121 ttgattcccc aagcgtaacg gtattcacct tttggcgttt tgttaacaaa gaggcgagcg
     9181 tgttgctcca tcactgctga aagaagacgg ctgacaccga taccgtaaca tcccatgatg
     9241 attggcacag cacgaccatt ttcatccaag acatctgctc ccatgcttgc tgagtagcga
     9301 gttccgagtt tgaaaatatg accgatctca ataccacgcg caaagttaag gacaccttgt
     9361 ccatctgggg aaatttcacc ctcacgaact tcacggatat ccacatattc tgcagtaaaa
     9421 tcacggcctg ggttcacacc agtcaagtgg tagccatctt cgttagcacc gacaactgca
     9481 ttgcgaacat cttgcacctt acgatctgca ataattttaa tattctctgg caaaccaact
     9541 ggtccaagtg aaccaaatcc tgcttgaaca acattcgcca cttcttcttc gctagcaacg
     9601 tcaaagaaat ctgctcccaa gtgatttttc aacttgactt cgttgagttg gtcatttcca
     9661 actagaaggg ctgcaacaag ctcaccatct gcaatgtaga agagggtttt aatcgtttgt
     9721 tcttctggaa cattgaggaa agctgcaact tcatcaattg atttaacatc tggcgttgca
     9781 acacgagtaa cttcttcttc agcgacaaca cggttgcttg gtttgtactc gtttgttgcc
     9841 atttctaagt tagctgcata gctagactca cttgagtaag caatggtatc ttcaccagag
     9901 actatccatt tgagcaattc tgccttgatt tcttcttgca cttctgcagg aatttcgtca
     9961 aatgaggcaa ctgacttgtc caagacaacc cagcggtcaa ggtctgtacg agcagatgta
    10021 atggccataa attcttggct atccttacca cccatggctc caccgtcacc aataatagcc
    10081 ttgaagtcta aaccactacg agtgaaaata cgctcatagg ctgctttgta ctcatcataa
    10141 acactatcca aactatcata gttagcgtgg aaactataag catccttcat gataaactca
    10201 cgtgtacgaa gaagtccatt acgtgggcgt ttttcatcac gatacttggg ctgaatttga
    10261 taaaggttga gtggcaattg cttgtaagat ttaacagaat cacggacaat agctgtaaag
    10321 gtttcttcgt gagttggacc taagataaag tctgattttt cacggttttt cagtttgtaa
    10381 aggtcttcac cataggtttc gtaacgacct gattcacgcc acaattctgc actaagaagg
    10441 gctggagcca acatctcaac agcaccaatc ttttcgaatt cttggcgcat gatgttttta
    10501 gctttttcaa tcacacggtt ggcaagtggt agataagaat aaacacctgc tgaaacttgg
    10561 cgaacataac cagcacgcaa cataagagca tggctgataa cttgagcatc gcttggcatt
    10621 tcgcgaagcg ttgggatagg cattttactt tgtttcataa tattcctcga ttatctaaaa
    10681 aagagtcgca taatgtcatt ccaagtcaca gcaatcatca agacaaccat gatgaccact
    10741 ccggccaagg tgacataggt ttcaatttct tgtttcaatg gtttgcggcg gatggcttct
    10801 aggatattga gcacaatctt accaccatcc aaggctggaa tcggaataag attaaaaatc
    10861 ccaatattga tggaaatcat tgccaagaag tacaagatat tttcaattcc atttttagca
    10921 gcatcactac ttgccttaaa gatagcaaca ggtccaccca acttgttcaa atccggttgg
    10981 aaaatcagat ttttcagagc tgagagaatt cggagagctg agtcagcagc agttgtaaaa
    11041 ccacctacaa acatggatag aaaatctgac ttaacccccg gttgaacacc tagaaggtaa
    11101 cgaccttgac tatcttcggg tgtaacagtg acttgtttgt cactcccctt ttcagaaata
    11161 gtcacatcca aagtcggtgc cgtcttatct ttggtttctg tttccacagc ttggatcaag
    11221 ctttcccagt tgctaacctc atgtgagcca atcttggtaa tttgtgccgt ttctggtact
    11281 cctaccttgg ccaaggcacc ttggggcatg atatggaact gattggtatc aacatctctg
    11341 acaccaccct gcataaagat taaaacccaa aaaacaacga cacctaagat aaaattgttc
    11401 ataggacctg caaaattggt aatcagtttg ccccagatag tcgcattttg atattgaaca
    11461 tctaaaggtg caatccgaac ctcagtacca tctgcttcca caaccgttgc atcgtgatcc
    11521 actgcaaatg ttttttcttc ttccagaacc aatcctttga taaagagctt gtcttcaaaa
    11581 tcaaactggg tcacctgcat agggagggct gtttgatcca attttttacc tgagagattg
    11641 atgcgtttaa ccttaccatc atcagcaagt gtcaaactaa caggcgttcc tgtcttgatt
    11701 tcagttgtat catcacccca accggccatg cggacatagc cacccagagg caagattcga
    11761 atggtatagg ccgttccatc cttgccaatg tgagcaaaga ttttaggtcc cataccgatg
    11821 gcaaattcac gtactaaaat ccctgatttc ttggcaaagt agaagtgccc gaactcgtgc
    11881 accactacaa taatcccaaa aaccagaata aaggttaaaa ttccgagcat agcgtttcct
    11941 ccgtcttttg attaaaagag tccaaataag tgcatgattg gaaatacaag caacatacta
    12001 tcgaaacgat ccaaaacacc accatgtcca gggataaatt tcccagaatc cttaacacca
    12061 aaatgacgtt tgatcgaact ttctagtaaa tcaccaaatt gtccagcaat gctaaagaaa
    12121 atagcaaaga ctgacatctt gtaaattcca tatggaagag caactgtact gtcaactatc
    12181 ataaagataa tggttactaa aattgctcct aaaataccac ccaaggcacc ctcaagggtt
    12241 ttattaggcg atacccttgg tgctaacttt cgtttcccat agttcatccc aacaagatag
    12301 gcaccactgt ctgtcgccca gacgatacac aaggctaaga gagccttgtc caaacctgca
    12361 acacgagcat ctagtaaagc attaaatcca aagcccacgt agaagctcat agcaagaggg
    12421 aaaaccgcat cctcaatcgt ataagacttg ctaaaaacgg tcgttcctaa catgattgaa
    12481 atcaaaacac tataggcaac cacattccca tcaactggca aaaaagtcag gtaattctcc
    12541 aagggaatgg tcaatgcaaa ggttgcaaag agggtcaaga ggccctccat cgtcatggtc
    12601 tctagacctc tcatcttcaa aagttcatgc atggctagca tggctatgat tccgattgct
    12661 atctgaagca agaggccccc aatcattaaa attggtagga aaatagccag ggcaatccct
    12721 gcaaacaagg ttcttttctg taaatcctgg gtcatatttc ctcctaaact cctccaaatc
    12781 ggcgatgacg acgattatag gcaagaatag cttcctgcaa ggccgcttcg tcaaaatcag
    12841 gccataaggt gtccgtaaaa taaagctcac tataggctcc ctgccatgga aggaaattgc
    12901 tcaaacgtaa ttctccacta gtacggataa tcaagtctgg gtctcgtaag tccttaggca
    12961 aatgctgagt aaagagatag ttaccaatca attcctctgt gatgtcacct gggttgattt
    13021 tggcatctaa aacatcctgg gaaatcaact taagcgcctg tgtaatctca gcacgtccac
    13081 catagttaag agcaaaatta agaatcaatc ctgtgttgtt cttagtcaat tcctcagcct
    13141 tggttaaagc ttcaaaggtt tgcttaggca ggcggtctgt ctccccaatc atttgaatct
    13201 taacattatt cgcatgtagt tccgggacat aattatcata aaactctact ggcaagttca
    13261 tgataaactt gacttcctga tctggacggg tccagttttc cgtagaaaaa gcatagaccg
    13321 taataacctt gacgcccagt ttgttggctg ccttggtcac ggtttgcaat gcttccatgc
    13381 ccgccttatg tccaaaaact cgcggttgca tacgtttttt agcccaacgg ccattgccat
    13441 ccatgatgat gccgatatga gcaggaacct gtgtcggaac ctctacttcc acagccttat
    13501 ctttcttaaa aaatccaaac atgatcttat tcctattcaa aaatctatcg tttcattata
    13561 ccatatttcc ccattttctt ctatcactaa gctatttatt ctcaggcacc atgcccattt
    13621 ttcaaaaaaa taagccgcct gattgggcga ctttattttt atagggagat tattatgaaa
    13681 aagttttagg agtttaagtt aaggtcttct taacttatga acttagtgta cactccctag
    13741 cttaaagttt ccttaagtat ttttaaaaat caaatttttc catttctcct gccaattttt
    13801 cttggataaa cgtgtttgat agagttccat tcggtcttca ttttctaaga aatgaggagt
    13861 tggacgaact tgaaaattca aaatatcctc caaaccataa ggtgcataga gttcaaaatc
    13921 tgattcttca ttcaagcgca gtccaactgc cgtacaccgt tctggatact tactcatagc
    13981 atcacgagaa ctggtatagg aagcagtgtg aggactgtgc tgatgcatat agacctgatt
    14041 tttcaattcc cactggtact gaggaaaatc ctctctcagc tttttctcca gtaataaggt
    14101 ttcctcataa gaaaaatctg gatcaaagaa aatcacatct atatctgttt catgatcaaa
    14161 aggggatttg tctgacaaaa gattccagat gaaatttctg acagaacctg ctgccaacta
    14221 cgagtctttc aaaccaaggt ctcggatgat cgtcagaatg gccatcatat ctggattttc
    14281 tctaaaagcc tctaagattt cttgcttatt tttcactgta ttcataacct aagtgctcat
    14341 atgccttagc agtcgccacc cgtccagacc gtgtccgcat gataaaacct ttttgaatca
    14401 agtaaggctc atacatgtct tcaactgtct cacgctcttc ggcgatattg acagaaagag
    14461 ttcctagacc aacaggtcct ccactgtaca tctcaatcat ggtacgaagg attttttgat
    14521 ccacatagtc caaaccttca tggtcaacat ccagcatagt caaagcctta tcggtaataa
    14581 tatcatcgat taccccattc cccattatct gggcaaaatc gcgcacgcgc ttgaggagac
    14641 gattggcaat acgaggggtt ccacgactac gtagggccaa ctcagatgct gcctcatgag
    14701 tgatttccat ctcaaaaata tctgccgtcc gctcgacaat ttctgtcaag tcagcatggg
    14761 cataatactc catatgacct gtaatcccaa aacgtgcccg tagtggattt gagagcatac
    14821 cagcccgagt cgtcgcacca atcaaggtaa aaggaggcaa ctccaaatga acactgcgac
    14881 tgccttcacc agccccaatc ataatatcga tgtagaagtc ctccatggca ctataaagca
    14941 cttcttccac tgacatgggt aagcgatgaa tctcgtcaat aaagaggaca tctccaggct
    15001 ctaactcatt caaaatcgct accaaatcac ccgctttttc gataacagga ccagacgttt
    15061 gcttgagatt gactcccagt tcattggcaa tgacaaaagc catggttgtt ttccccaaac
    15121 ctggaggccc aaataagagg acatgatcca gcgcttcatc tcgcattttg gcagcttcta
    15181 taaagatttg aagctggtcc ttaaccttat cctgcccaat atattcacgt aaatactgag
    15241 gacggagcgt gcgttctact aactcctcat cccccattat ctcattatct aaaattctac
    15301 tcatggctct attatatcaa aaaaccaagc cacaaacaaa aaagccacct gattgggtga
    15361 ctcctaagtt tagcacttat gtggtataat attctacgac actgctacac cgcctacgaa
    15421 aggaggtgag atagcccatg atggaattag tactcaaaac tatcatcgga ccaattgtgg
    15481 tcggtgtcgt tcttcgtata gtcgataaat ggctaaacaa ggacaaatag tgtcaaaaaa
    15541 gaccccaagc ttatttggtc gtgagcttgg ggtcttttct agcctatgat atagaactag
    15601 tactcaattc ctttttatta tcccatagtt cacgaatttt gtcaaaggtt tacattttct
    15661 tcaaccgctg tacgacaaga cggttaagat taagagaacg ttagggattc tatcaatttc
    15721 atagaaattt tgatttcgta aacgaagaga caatcttaca tgtcacttct catttaatac
    15781 gccactacta gacaagcaaa atcattatta cagtagttcc agttcttcaa ttaacagtca
    15841 cttacaatca aattgagttt gaactagctg aagcgaccac agacctattt cttagttata
    15901 ttcgctaaaa aagtccccgc caattccccg accaaaatcc gaaaaatatc gaaaaattat
    15961 ttttagaata gtccccaaaa gcctgaaata gagctaaaaa actccacctg attgggtgga
    16021 gttaagggag attattatga aaaagaaaag
//
LOCUS       DAVMMR010000047        15518 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMMR010000047 DAVMMR010000000
VERSION     DAVMMR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.047"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(184..1806)
                     /gene="groL"
                     /locus_tag="VYS74_001513"
     CDS             complement(184..1806)
                     /gene="groL"
                     /locus_tag="VYS74_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV3789527.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1822..2106)
                     /gene="groES"
                     /locus_tag="VYS74_001514"
     CDS             complement(1822..2106)
                     /gene="groES"
                     /locus_tag="VYS74_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV3789528.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2279..2674)
                     /locus_tag="VYS74_001515"
     CDS             complement(2279..2674)
                     /locus_tag="VYS74_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV3789529.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2752..3513)
                     /locus_tag="VYS74_001516"
     CDS             complement(2752..3513)
                     /locus_tag="VYS74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3789530.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3546..4175)
                     /locus_tag="VYS74_001517"
     CDS             complement(3546..4175)
                     /locus_tag="VYS74_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV3789531.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKKENG
                     ETVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEI
                     VEMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPG
                     ELRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4191..4508)
                     /locus_tag="VYS74_001518"
     CDS             complement(4191..4508)
                     /locus_tag="VYS74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV3789532.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4505..4804)
                     /locus_tag="VYS74_001519"
     CDS             complement(4505..4804)
                     /locus_tag="VYS74_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV3789533.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5089..5280)
                     /locus_tag="VYS74_001520"
     CDS             complement(5089..5280)
                     /locus_tag="VYS74_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV3789534.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5441..6115)
                     /locus_tag="VYS74_001521"
     CDS             complement(5441..6115)
                     /locus_tag="VYS74_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789535.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTAKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6121..6567)
                     /locus_tag="VYS74_001522"
     CDS             complement(6121..6567)
                     /locus_tag="VYS74_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3789536.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6681..7184)
                     /locus_tag="VYS74_001523"
     CDS             complement(6681..7184)
                     /locus_tag="VYS74_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3789537.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISVACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7181..7543)
                     /locus_tag="VYS74_001524"
     CDS             complement(7181..7543)
                     /locus_tag="VYS74_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV3789538.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7609..9474)
                     /locus_tag="VYS74_001525"
                     /pseudo
     CDS             complement(7609..9474)
                     /locus_tag="VYS74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9484..9954)
                     /locus_tag="VYS74_001526"
     CDS             complement(9484..9954)
                     /locus_tag="VYS74_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV3789539.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(10078..10794)
                     /locus_tag="VYS74_001527"
     CDS             complement(10078..10794)
                     /locus_tag="VYS74_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3789540.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11677..13092)
                     /gene="ply"
                     /locus_tag="VYS74_001528"
     CDS             complement(11677..13092)
                     /gene="ply"
                     /locus_tag="VYS74_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV3789541.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13103..13435)
                     /locus_tag="VYS74_001529"
     CDS             complement(13103..13435)
                     /locus_tag="VYS74_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789542.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13506..14114)
                     /locus_tag="VYS74_001530"
     CDS             complement(13506..14114)
                     /locus_tag="VYS74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789543.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14127..14584)
                     /locus_tag="VYS74_001531"
                     /pseudo
     CDS             complement(14127..14584)
                     /locus_tag="VYS74_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
     gene            14709..15162
                     /locus_tag="VYS74_001532"
                     /pseudo
     CDS             14709..15162
                     /locus_tag="VYS74_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4541 a         3579 c         2805 g         4593 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttc atcgctctga atatcaaaaa agaaaggaca
       61 aaatttgtcc tttctcgagc ttagcttttc ttcaacccac tacagttgac aaagagccta
      121 taaaaagaag ggttagtcat tccctccttt tgtgtttttt ataagttgtt ttctatagaa
      181 agcttacatc atcccgccca tcatacttgg atccattgct ggagctgggg ctactggttc
      241 tggtttattg gctacgactg cttctgttgt caaaatcaag ctggctacag atgctgcatt
      301 ttgtagggct gaacgactca ctttaactgg atcaatgata ccttgatcaa tcatgttaac
      361 ccactcgcca gttgctgcgt tgaatcctat accaagctca gcatttttca aacgatcgat
      421 aacgatagat ccttcaaatc ctgcattgtg agcaatttga cgaacaggtt cttccaaagc
      481 acggagaaca atattacgtc ctgttgcttc atctcctgtc aattccaagg tagcaacagc
      541 tggaatcaca ttggcaagag ctgttccacc acctgcaaca ataccttctt caacagctgc
      601 acgagtagcg ttgagggcat cttcaatgcg gagtttcatt tctttcaact cagtttcagt
      661 tgcggctccg accttaataa ccgctacacc acctgacaat ttggccaagc gttcttgcaa
      721 tttttcacgg tcaaattcag aagttgtagt ttcgatttga gacttgataa ccgcaacacg
      781 gtgagaaatc gcttcaggat ttcctgcacc ttctacaata accgtgctat ctttgtccac
      841 ggtcactctc gctgcttgac caagagcttc aattgtcgca tctttcaact caagaccaag
      901 gtcttctgtg ataactgttc cgcctgttaa gatggcgata tcttcaagca tggctttgcg
      961 acggtcacca aaaccaggtg ccttgactgc tactacgttg aaggttccac gaatcttgtt
     1021 caaaacaaga gttggaagag cctcgccatc cacatcatcc gcaataatca agagtggacg
     1081 attgctttgg agaatgcttt ccaaaagtgg caagatttct tggatattgg aaattttctt
     1141 gtctgtaatc aaaatgtacg gattttcaag gtcagccacc attttttcgc tatcagtcac
     1201 catgtactgt gaaaggtaac cacggtcaaa ctgcattcct tctacgactt caagctctgt
     1261 ttccatacca cgtgactctt cgatggtgat gacaccgtct ttgccaactt tttccattgc
     1321 ttcagagatg tactcaccaa ctttttcaga acgagaagat acggctgcaa cttgagcgat
     1381 agcttcttta ttggcaacag ggatggcgtt gtttttcaaa gcttcaactg ctgcggcaac
     1441 tgctgtttca atcccacgac gaataccgat tggatttgca cctgctgtga cgtttttgat
     1501 tccttcacgg acgatagctt gggtcaagac tgttgcagtc gtagtcccgt cacctgcgat
     1561 atcattggtt ttagaagcta cttctgatac taacttagca cccatatttt caaaatggtc
     1621 ttccaattcg atttctttgg caatggtcac accgtcattg gtaatcaagg gtgaaccgaa
     1681 tgacttttca agaacgacat tgcgaccttt tggtcccaag gttactttaa cagtgtctgc
     1741 aaggatatcg acaccacgaa ccatggctga acgggcatct gatgaaaatt taatttcttt
     1801 tgacatactt actttctcct tctattcctc aatgattgcc aaaatgttag cttcgcctac
     1861 gatgatgtac ttttcatcgc catctttgac atcaagacct gcgtgggctt caactaagac
     1921 acgatctcca gttttaacac ttggagcaac caagtcaccg ttcaaggtac gaacaccttg
     1981 tccagtagcc acaacttggg ctgttttggt tttttcttgg gctgaacctg cgagaacaaa
     2041 gcctccaaca gtttgttctt tttcttctac ttttaagacc acacggtccc ctaatggttt
     2101 caacatctga tttcctccat aatctgattt cctccataat gagatagata ttattagcac
     2161 tctttatacc tgagtgctaa ttcatagttc tattgtatca cttagtcaga aataatcaag
     2221 aaaaaagtct gactttctca agataaaaag cctgagacca actcagactt tttaattctt
     2281 aaaatggcaa ttcttcctct tccaagacca aatctgccaa atcttggcct gcattatttt
     2341 cacgcatagc acgttgggca cgactttcca agagttggaa tcctgtcaca aggacttcgg
     2401 ttacataatt catctgacca tttttctcaa agcgacgggt acgcaattct ccatcaacgg
     2461 aaatgagact acctttggtt gcgtagcttg ccaaagtttc tgctagtctg ccccatagaa
     2521 ccatattgac aaaatcagct tcacgttcac cgttttggtc tttgtaacga cggttgacag
     2581 cgatagttgc tcgcgctact gacttgtcat tgttggtttt gtgcaattct ggtgtagacg
     2641 ttaaacgtcc aatcaagata actttattat acatattttc ttcctcctac ttatctattc
     2701 gtaggaaatc aaaaaaagtt acagaaattt gtaacttttc gagaaaattt tttatttttt
     2761 atgaaccatg aaacctgtcg cctgttgatt ggccataatg gtcatatctg taatctgaac
     2821 acgacgaggt tgactagtca catagactac tgtatctgca atatcctgag cttgcaaagc
     2881 ttctattcct tggtaaacgg acgcagctcg ttctttatca ccatgaaaac gcactgtaga
     2941 aaaatctgtt tcgacaattc caggctgaat ggtcgtcacc ttgatatccg ttgcgatggt
     3001 atcaattcgc agtccatccg aaaaggtctt aaccgctgcc ttggtagctg agtaaacagc
     3061 ggcaccagca taggcgtaga ttcctgcggt tgaccccata tttataatat gaccttgatt
     3121 gacttttatc attgctggca agaaacagcg agtaaccgcc atcagacctt taacattggt
     3181 atccaacatg gtcaacatat ccaactcctc atagtcttga tagggagcca agtcaagagc
     3241 cagtccggcg ttgttgacca agatatcaat cctccctatc gtttctagaa tatcagagca
     3301 aacagtcttc accatgacca tatccgtcac atctagtgga aaagtccaga cggtttgatt
     3361 tggaaagctt actgcaaact ccgacttgag gatttctaat ctgtctatcc gtcgtcctgt
     3421 tagaacgaca tcctcaccct gctccaaata agcacgcgca atcgcttcac cgattcctga
     3481 ggttgctccc gtaatcacta cattttttgc catcttattt ccttctatca gatgctgaca
     3541 agttcctagg cagtccagtg tttcgctggg tcgaacggtg ttccgacaac ttggtcttct
     3601 gataattcaa gcaccccacg tttttgcgga gcatttggca aatgcaattc acgaggacta
     3661 cacatcatcc caaaactctt ttcaccacga agttcgcctg ggaaaatgaa attccctttt
     3721 ggcatcatgg caccaggaag agccacaatg gttttcaacc caacacgcgc attaggtgct
     3781 cctgcaacga tttgcactat cttgtcactt gcgactgcaa cttggcagat gttgaggtgg
     3841 tcactgtctg gatgagctac catctcgaca atttcaccaa caacaaactt aggttcctta
     3901 tcattaacaa tttcttctgt aaaaccttcc gcctgtaatt cttggttcaa acgagcgact
     3961 tgctcatctg ataaaaagac ttgaccgcgc tctgcaattt caaacaagct cgaaacctca
     4021 aaaatgttcc aagctactgt ttccccattt tcttttttga ggaaaacacg ggctaccttg
     4081 cctttgcgtt ccacattcag tttggcatct ccgctatttt tcacgatgac cataaggaca
     4141 tcaccgacat gttctttgtt atacgtaaaa atcattgttt cctttttctc ctatttcaat
     4201 cctgctaaaa aatcgttaat ttgctcctta cttttacggt cgcgattgac aaaacgaccg
     4261 atttccttgt ccttttctag aacaacaagg ctaggaattc cgtaaacatc ccagagtttg
     4321 gccaaatcca tatactgatc tcggtccatt cgaataaagg tgaactctgg attggtctcc
     4381 tcaatctctg gtaaggcagg ataaatataa cgacaatcgc cacaccagtc tgccacaaaa
     4441 aggaagacct tcttgcccgc tttttccact aaagatgcta attcttctaa acttgctggc
     4501 tgtatcataa gacttcctcc tcatagacta ggtcttcatt ttcatagaca aaggtataat
     4561 gacggccatc ctcaaaaatg acgccaccaa ccaagctctc cagactgctt tcgtaaactt
     4621 gaacatagag ggtcgcaatt tcccccatgt cggaaaaatg gtctcgcaca atctctgtca
     4681 actcttcctg agtcttcatg agcttacggt catctgcaac ttttttcgtg gcaagagcaa
     4741 ggcttccgat acctagcaga gccaagcctg ccatccacat ttttttagct ttcataccat
     4801 tcattttaac acaaaaaagg cttcaggaca aatgaggaag cagcagaaaa gcaagtaaaa
     4861 agcctcttcc tttaaggaaa aggacttctt atactaagac caaactagaa agctagccgc
     4921 aggctgctca aaacacggtt ttgaggttgt agataaggcg acgctgacgt ggtttgaaga
     4981 gattttcaaa gagtattatt cttattgcca ggcacctaag ttgccaacgt agtaaccatc
     5041 aggtgtgtag gtattgcgaa acatcttacc tgatgaagcc agataatact acttgccatt
     5101 gtctttgacc caatcattcg caatcatgga accagaagaa cttacataat accattctcc
     5161 cttgtcataa acccaagtac tgactttcat ggttcctgag caattaaagg caaaaaaact
     5221 gtccaatatc attcgttttt taaaagcatt tgacactaca tcatctacgc tgtagtacat
     5281 agatacatca aacgtaaaag ccaaaatatc gaaaaatata caacttttaa aggcagcatt
     5341 tcatttttta aagtctaaca accatagaac tgaaatcaga gcaaggcatc aatttcctac
     5401 tcttagtacg tctattttga tggttgaagt gaagattttt ttatttaacg tagacaattt
     5461 ccaacttccc aaaagccaca tctccacgga tagtcaaggt tttgctggtt ggggctacag
     5521 gagcgtcagc cttagctgca ccaaaggagg tttctacttt caaatctaca cgccagtgtt
     5581 gtggaacata gacggttgca tttccgaagg ccacttcaat atttaaagtt gcaaaatcac
     5641 ctagcatctc tgcattatca tagtagattt tagcatcccc aaaagcgact tccacttggt
     5701 catctacgag atcttgatct tgcttataga aggtcccgct accaaaagcg acttccttag
     5761 ccgtgacgac tgtttttttc ccattgtacc accatttttt ttcattccag aacttacttg
     5821 aatgcgtcag ataaccaaca ccaagtacca ccaagatgct agcccaaata agagaatgat
     5881 tggtaactgg taacaagtca taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa
     5941 aaactgccga agtgagatga cgtctaagga tggactcaat ggacttataa gcaaaaaaaa
     6001 caatacctag taaaggccat attttaccat ccaaagaagg aattccaaaa ttcccttgca
     6061 gcaagatcca agctgctaaa accaataaaa caataccaaa tgctttcttt ttcatgtttt
     6121 ttacctcatg tttcttagat tttcttttag gagggattgg taatgccgtg agacatgaac
     6181 ctccttgtgc gtctgataaa aggaaatggt gcccgttcct gaaaaggact tgtccactga
     6241 gtaaatctga cggatgtttg cgatcgttga cttggaaact cgattaaaat agcgaggcaa
     6301 gatagactcc aactcataga gcttgaggcg aacctcataa gcttccttct ggttatgagc
     6361 gtagatcttg ctcccttctg tttcaaagaa gagaatttct gacaggtcta gataatattc
     6421 acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga gccgttgcaa
     6481 gtctgcaatt tcatctgtca aagtgggggt cttgatgaca atttcagtct cctctaaatt
     6541 gccgtcaatc tcaattcgta acttcatcac atctcctttc tgactctact atatcaaagc
     6601 taagttagga atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta
     6661 ggactagctt aacttacatg tcagaatagg aagaaaagga atccccacac caaaccacaa
     6721 gcatagccaa ccaagacatc cttgggataa tgaacacccc ctaaaaccct caccaacccc
     6781 agcaaagctg aaaagatcaa gcatactaag ccaataggta gactagcatg cagacaggcc
     6841 acggagatga tagttgccga aaagacatgg cgactgggca tcgaccgtcc cgaactatcc
     6901 ttatcaagaa ggggacagat atcccaagtt tcataaggtc tcgggtgatt gattttctta
     6961 cgaaacaggg acaaggccac aaaacctgat gcaggaataa gcacataaac cccaacttgc
     7021 tgaccaagtc ctagttgcag gtaagtggtg actagcaaga tcagatagac caggggcatg
     7081 gcgactgtca tcaaacgatt gaaactgcgt aataggaata aaagaattgg acgactagtt
     7141 agtctggagc tgatatttcg ataccattct tgataatttt tcatttatgc ttctcatact
     7201 tttctagaac ttgttgagat gttttcccct tggcaagcca atccactagc ttgtccaagc
     7261 gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat acggacaccg caaatcttcc
     7321 ctgtaattgc aattcgttca gggacataat atggtgcttg tctaaaaaaa tctccgtaag
     7381 taacatccct atccagacaa gcaagaacat cactcacttc ataccctgtc aaccaactgg
     7441 tcacttgatg aactgactct gcctttccac cctttcgctc aacttttgca atcaaggcta
     7501 agtagacagc agcaaatttc atattgtata atttctgact catacttctc tctttctatg
     7561 tgtactagta taaataagaa aaagaaggcc gtcaagcctt cttttgattt attcttctgc
     7621 ttcatcttct gtaaattgac tattgtacaa gtcagcgtag aagccacctt gcgccatcag
     7681 ttcctcatag ttgccttgct cgatgatatt tccatctttc atgaccaaga tcaagtctgc
     7741 atttcggatg gttgacaagc ggtgggcaat gacaaagaat gtgcgtcctt ccatcaaacg
     7801 gtccatggct ttttggatca attcctctgt ccgtgtgtca acagaagaag tcgcctcatc
     7861 caaaatcaaa agcggtgcat ccttaagaag ggcacgagca atagtcaata gttgtttttg
     7921 tcctacagac aaggtcacgg tgtcatccaa gatggtatca tagccatctg gcaaggtcat
     7981 aataaagtgg tgaattccca cagccttact agcttccatc attcgttcat cactaatccc
     8041 tatttgatta tagatgagat tgtctcgaat agttccttca aagagccagg tatcctgcaa
     8101 gaccattgaa aaggcatcat gcacttctga acgcgtcata gccttggtat ccacaccatc
     8161 aatgtgaata cttcccttat caatctcata gaatttcatc aaaagattga caatggttgt
     8221 cttaccagtc ccagtcggcc caacaatggc aaccttttga ccagcatgag ctgtcgcaga
     8281 gaagtcatag tcttgaacat tgacaccgtc caccagaatt tctcctgctg acacgtcgta
     8341 gaaacgtgga atcagattga ccagagttga tttaccagaa cctgttgacc caataaaggc
     8401 cactgtttga ccagtttctg ctttaaagct aacatgttca ataactgcct ccgaatttgc
     8461 cgcatagcgg aaggtcacat ccttaaactc gacctgacct ttgaagtttt catcagtcag
     8521 ctgcacttga acagggtttt ggatagaaga atgcaaatat aaaacttgat taatccgctt
     8581 agcagagacc atagttcggg gaagaacgat gaagagtgct cccatgagaa ggaagcccat
     8641 gacaacctac atggcataag acatgaaaac aatcatgtca ctaaagagag gcagacgcgc
     8701 tatcggagca gcgtcgttaa tcacataggc cccaatccag taaatcgcca cactcaaacc
     8761 acttgaaatc cccatcatga taggattcaa aatagccata agacggttga caaacaaatt
     8821 caaacgggtc aattcatcat ttactgctgc aaatttttca ttttgataat cctctgcatt
     8881 gtaggcacga acgacacgaa tacctgttaa actctcacga gtgatactgt tcagtttatc
     8941 tgtcagcccc tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat
     9001 caggacgttg acaatcactg ccacaagtac ggcccagagc cagtattctg aatgacctaa
     9061 aatcttccca atagcccaga tagccataat tggaccacac gttaccactt gcaagcccat
     9121 agtaatcaac atttgaactt gagtaatgtc attggtagta cgcgtcaaga ggctaggaat
     9181 tgaaaatttc ttaatctctg tctgcgagta atccaaaact cggttaaaaa tatcacttct
     9241 cagcctacta gtataagaag ccgccactcg ggatgcaaaa aatccaactg caactacgga
     9301 caagaaggca agaaagagca ttcccatcat catgcttgcc ggctgccaca actcatctaa
     9361 attagttcct tgactaccta gcaaatccgt aattttcgag atataggtcg gcacttccaa
     9421 ctctagatag accgaaaagc aagtaaagag aatggctagt aaaatcatcc ccattctttt
     9481 ctactaattc ttttggctaa tttctttatt ctctcctcct attcccttga tatttgcctg
     9541 tagttgaccg agaactttct caaaaatcag taattcatct tcatcaatgt cttccatcaa
     9601 ctgcttgtct atgcgttcaa aaaaagcctt aacctgttgc atctgagaac gtgctttgtc
     9661 cgtcagacga acaaacttag cccgcttatc aacaggactc gcctccaatt ccaccaaacc
     9721 attttgcact atacgcttaa ccagattact agcaacagac ttggtaatat tgagttcctg
     9781 ctcgatatct ttaatcaaga ccaagtcttg gtttttctcg cgattatcca aaaaacgcac
     9841 aacctgacct tgcggcccac ccataaattc aatgccgcaa cgtttggctt ccttttgcac
     9901 catcaggtga atttgatgac caaaacgctt aaagactaac atcggtttac ccataatctc
     9961 ccccttctaa ataaaaatag ttctctggag aataattaaa tttctatgag aactattttc
    10021 ttgattaaaa aaatcccaag tgattttctc acttaggatc atgttctata ggttaaatta
    10081 aaacccatct acgttcgtat aaatcttttg gacgtcttcg tcgtcttcaa gaacgctgta
    10141 aagtttttca aaggtttcaa ggtcttcgcc tgacaattcc acttctgact gaggaatcat
    10201 ttccaattca gtcacttgga attcttcaat accagactca cggagggcaa cgatagcctt
    10261 gtgaaggtca gttggcgctg tgtaaactgt gattgtacct tcttgtgctt ctacgtcatc
    10321 cacatccaca tccgcttcga gcaattgctc aaagactgcg tccgcatctt cacctccaaa
    10381 tacaataaca cctttgttgt caaagaggta agaaacagaa cctgaagcgc ccatgtttcc
    10441 gccgttttta ccaaaggctg cacggacatt ggctgctgta cggttgacgt tagaagtcaa
    10501 agtatccaca attagcatag agccatttgg cccaaaacct tcgtaacgtc cttctgtaaa
    10561 ggtttcgtct gtgtttcctt tggctttatc aatcgcttta tcgataatgt gttttggcac
    10621 ttgggcttgt ttagcacggt cgataacgaa tttcaaagct gagtttgatt ctggatctgg
    10681 atcacctttt ttagctgcta catagatttc tacaccaaat tttgcatata ctttagagtt
    10741 ggctccatct ttagccgttt tcttggctac gatattggcc catttacgtc ccattaggaa
    10801 tctccttttt tcacatttta atctttctta ttataacaca agtttttttg attttcacta
    10861 gaggaaatgg attttattag caaatcaagc taggatagca ctttacctgc taagatggtc
    10921 ttgcctttct atctttttat caacagacac tcatccacat tcaaaaaaca aactagacca
    10981 ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt cagctcaaat tactgtttga
    11041 agtttgtaga tataagcgac aaaaacaatc atactgcacc ttttgttgac agtctactcc
    11101 agacatatca tagttcaagt aaatactttg aaatccaaca gttcttatag gcgctattgt
    11161 attctaagaa atcaatagaa gagtttctaa gcaaatctct aatactcaat aaaaatcaaa
    11221 gagcaaacta gaaagctagc ctcaggttgc tcaaaacact gttttgaggt tgcggatagg
    11281 gctgacatgg tttgaagaga ttttcgaaga gtataattta cgtgttccca agatggagaa
    11341 gttagactag tacactggca cttctaaacc attgctagca attgatttgt tcatatttaa
    11401 tttcattttt tccataaatg ggtattagat ataaacagca aaatatttcc gatacgtgtc
    11461 gttcttgaat ttccaatcat ctaaaacaag taaaggataa tcaatcccct gtatatcaag
    11521 gaattggcta ccctttttac ttttttacac attctgtttg atagattcat tttaacatca
    11581 cgagcatact ccaatggaaa tcgctaggca agagataaac tttcagatat ccgcagagag
    11641 atcatcgcct ctttttgtcg caagcattct cctctcctag tcattttcta ccttatcttc
    11701 tacctgcgga tagagagttg ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa
    11761 atcggttttt tcataaaccg tacgccacca ttcccaagca agcccggtac actctctaat
    11821 tttgacagag agattacgaa cattcccttt taaaggaata ctagtggtaa agtgagccgt
    11881 taaatcctgc ccatttctgt cccaagcctt aggagtcaag acttccttac cttgatgatc
    11941 ataggataat tcattccaag taatataata ttgggcaaca taggcaccac tatgatccag
    12001 cagtaaatct ccgtttctgt aagctgtaac cttagtctca acatagtctg tactattttg
    12061 aaaggtcgca actacattgt cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg
    12121 atgatctgct gtaaagcgac tgccttcttg aatcaagtcc tctaccatat ccaccttgcc
    12181 tgttacaact cgggcacccg aacttgggtc gccccctaaa ataaccgcct tcacttctgt
    12241 attgtccaaa atctgcttcc actctgtctg aggagctacc ttgactcctt ttatcaaagc
    12301 ttcaaaagca gcctctactt catcactctt actcgtggtt tccaacttga gatagacttg
    12361 gcgcccataa gcaacactcg aaatatagac caaaggacgc tctgcagaaa ttcctctctg
    12421 ttttaaatcc tctaccgtta cagtatcttg aaacacatct cctggatttt taacagcgtc
    12481 tacgctgact gtataataaa tctgcttaaa attaacaatc tgaatctgct tttcgcctga
    12541 atggacagag ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt cagaaccaaa
    12601 cttgaccttg agttgttcca tgctgtgagc cgtgattttt tcatactgca ttctagctgg
    12661 gacattattg acctgaccat aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc
    12721 gcgaacactt gaattgctgg ggtcttccac ttggagaaag ctatcgctac ttgccaaacc
    12781 aggcaaatca atactataag tcatcggagc acgatcgacc gcaagaagag tgggattatt
    12841 ctctaacaag gtctcatcca ctacgagaag tgctccagga tagaggcgac tgtcgttggt
    12901 agctgttaca gaaatatcac ttgtatttgt cgacaagctc cgcttctttc tttcgataac
    12961 aacaaactca tcgggtagct gattaccctc tttgatgaaa cgattttcaa tactttctcc
    13021 ctgatgggtc aagagtttct ttttatcgta attcatagct agtataaagt catttactgc
    13081 tttatttgcc atcttctacc tcctaataag ttcctggatt gagttgcata aactcagact
    13141 tgttcagcga aatcagccgt ggttggacta agtaatccaa aatttcctcg tacaattctt
    13201 ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg aataaccgta ttatccaaca
    13261 taaatacctt atctaagtca atcaaggttg gtcttgtaaa aggattacga gctagatccg
    13321 gctcttctat cataaagttc ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct
    13381 gatttttaac caactctttg tttttagtca tgctgttgtc aatacagata tacatatgat
    13441 tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct acaacatctc
    13501 ctctcttaat caagcaagag cacccccttt tctcctaatg taacatagac aggattgacc
    13561 aagtcttctg attgactcag aatttccaaa gtttgagttt ggcgcgctgt caatttagta
    13621 gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa ccttgacaat ataatccttc
    13681 tccaaagctg actggtaaat ccacatcaga tgttgtctgt cctgagaact caagagagaa
    13741 ggattttcaa gcctcccgat agtctgataa aaatcaaaaa caggagctaa ctcctgccaa
    13801 tctgattggc tagttgtcaa ggctagaaaa agggctttgc gagctgatac ttcttggtta
    13861 gccttgagag ttactttccc ctccaagttt tttagaaatc gggaaactcc agaaagcaaa
    13921 tttttctcta actgcgagaa ataaaaacct ttcgttccca gacataagtc tttcatgtcg
    13981 ctttctctag caaataagag ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg
    14041 gtcgcgctca tcttttcctt atcggcttct ttttttaacc agagcaaggg cgacaggtag
    14101 ctggattgag acatttcctc tacctcctac tcttttttaa ctggagcatc tgcactagct
    14161 gccacttctt ttgactggat actttcccac tggttaatct cctctgagat aagaccttcg
    14221 catgtcttga caaatagggc aaaagccttg gtctttcctg catatttctc cgtttggcat
    14281 tgatagagga atttttcttt ctccaggagt tgcgcagttt tttggtaaga aatccaattt
    14341 tcctttgcat tatacaaatt gataatcccc tcacacagca agccgagact ggataaggca
    14401 accgaaatca aacggtagcg atcacctggc ataggaatag cacaaaagac agctatgagg
    14461 aaacctgcca cgatttctgt tatttttaat accttatagc gcctacgatg ttgaacgctt
    14521 ttctttaaaa atgagctatc tgtacgtcta atcgctctgt caggtacatt tcttctggcg
    14581 tcatattcgt aactcctttc atttactttg ataatcatag tataacatat ttgaatatca
    14641 aagtatagta aaaactggac tataacttat tagacttcct acgaaacaaa atatggtata
    14701 gtagttctat gaatgatgaa gcaagtaaac aactaactga tgcacgattt aagctccttg
    14761 ttggtgttca gcgtaccact tttgaagaga tgttagctgt attaaaaaca gcttatcaaa
    14821 aaagtcggac aagctggtaa aatcttggct gacagtggtt atcaagggcc catgaagata
    14881 tatcctcaag cacaaactcc acgtaaatcc agcaaactca agtcgctaat agctgaagat
    14941 aaagcctata accatgcgct atctaaggag agaagcaagg ttgagaacat ctttgccaaa
    15001 gtagaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
    15061 cgaatgaatt tgattgctgg tattatcaat catgaactag gattttggtt tcgcaggaag
    15121 tctattatat atgcacagac atatgaaatc tttttgagat agcagttatg atatatttat
    15181 ccattcacaa catcttctgt actaatacta caatattggt acattttgat tacacaatcg
    15241 tttcaaaaaa tatgatggaa gcctgagtat tttatagtaa ttcccttcct atcaactaga
    15301 tcaaccagtt gagtgttgaa tgttgaaaca ccctagcctc agcaaaggac tatgatcagc
    15361 atttcctagc caataggacg ctccaaatca cctattctaa gtccatcact agcttggtca
    15421 gtaagataca gactgcaagg cgagactgac gtggttgatg agattttgga agagtattac
    15481 aatcatagat tcaccagtag acttgaccgc agatttaa
//
LOCUS       DAVMMR010000048        14965 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMMR010000048 DAVMMR010000000
VERSION     DAVMMR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.048"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VYS74_001533"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VYS74_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV3789544.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1320..2024
                     /locus_tag="VYS74_001534"
     CDS             1320..2024
                     /locus_tag="VYS74_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3789545.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2017..3366
                     /gene="vicK"
                     /locus_tag="VYS74_001535"
     CDS             2017..3366
                     /gene="vicK"
                     /locus_tag="VYS74_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV3789546.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3368..4177
                     /locus_tag="VYS74_001536"
     CDS             3368..4177
                     /locus_tag="VYS74_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3789547.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4268..4510
                     /locus_tag="VYS74_001537"
     CDS             4268..4510
                     /locus_tag="VYS74_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV3789548.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4512..4766
                     /locus_tag="VYS74_001538"
     CDS             4512..4766
                     /locus_tag="VYS74_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV3789549.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4841..6712
                     /locus_tag="VYS74_001539"
     CDS             4841..6712
                     /locus_tag="VYS74_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV3789550.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVT
                     QHNSRKTWYYVPLQDFTVNSDIDWTQSVADIDLQLDQKYDLSPEEIAFIENHVREMD"
     gene            6877..10131
                     /locus_tag="VYS74_001540"
     CDS             6877..10131
                     /locus_tag="VYS74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3789551.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(10517..11503)
                     /locus_tag="VYS74_001541"
     CDS             complement(10517..11503)
                     /locus_tag="VYS74_001541"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV3789552.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            11697..14165
                     /gene="gyrA"
                     /locus_tag="VYS74_001542"
     CDS             11697..14165
                     /gene="gyrA"
                     /locus_tag="VYS74_001542"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV3789553.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            14165..14908
                     /gene="srtA"
                     /locus_tag="VYS74_001543"
     CDS             14165..14908
                     /gene="srtA"
                     /locus_tag="VYS74_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV3789554.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         4811 a         2528 c         3258 g         4368 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagcattga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggtccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttt ggtataatag tagagaaaaa ggtgaacata
     1321 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
     1381 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg ctagagcaat
     1441 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
     1501 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
     1561 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
     1621 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
     1681 ctatgccagt agatggtcag gaagcagata gtaaacctca acctatccaa attggggatt
     1741 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
     1801 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
     1861 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
     1921 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
     1981 tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
     2041 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
     2101 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
     2161 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
     2221 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
     2281 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
     2341 gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
     2401 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
     2461 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatatc
     2521 aatggtgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
     2581 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
     2641 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
     2701 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
     2761 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
     2821 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
     2881 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
     2941 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
     3001 acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga tgggggtaaa
     3061 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
     3121 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
     3181 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatt
     3241 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
     3301 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
     3361 gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt ccagtggaaa
     3421 ttctttttat ctggaaacct caaaaaagaa gcttttagtg gatgcaggct tgtctggcaa
     3481 gaaaattacc agtctgctag ctgaaattaa ccgcaagcca gaagacctgg atgccatctt
     3541 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
     3601 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
     3661 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cttttggaga
     3721 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
     3781 tatgaaggat gacaagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
     3841 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
     3901 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttggtca
     3961 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gcactaagaa
     4021 gatttacctt gggcatttat ctaaggaaaa caatatcaag gaactggctc atatgaccat
     4081 ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg tttatgatac
     4141 ctcaccagat accgcaacac cattgacaga gatatagaaa gaacgctgag aaggtgttct
     4201 ttttatattg actgaacacc taaaaagtaa tacaatggtg ttaccattaa aaaagggagc
     4261 acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat ttatgaaagc
     4321 aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa ctcttgaagc
     4381 cctagaagat gaatacgatg ctcgtgtggc agatttagcc tatcaagagt atttagaaga
     4441 cttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt taggcttgaa
     4501 ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa ttgaaaaaat
     4561 tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt ttggaagacc
     4621 caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc tatagaattg
     4681 gtaattatcg agttatcgta caaattgtag atgatgaatt agtcgttgct actctagaag
     4741 ttggtcatcg gagagatatt tattgaatta cttttttcaa gtaaattttc attctttaaa
     4801 atatgatata atagggctta caatataaga gaggttgctg atgacaatag atattagtga
     4861 agaaagtttg gctaaagaat cggctgactt attaaaaatt cttttaaaag atcgaacgac
     4921 aaagaaatca attgtttggg ctacgcattc ttatgaattg ttaggaaagg gatttgctcc
     4981 aagtgatcgc atcaatccaa gtaaggttac tggaaacttt gccaacttga ttcagcccag
     5041 atctgaaaaa tccaaatatg aacaaaaaga tcggacaaaa ataagggcag aagtgtttac
     5101 accaacgtgg ttggtcgcaa agcaaaatgg atatgtagaa tcgaaattag gctcgttaag
     5161 tcttgaggag tatgtggact tacgatggtt agaggtaact tgtggtgaag caccttatat
     5221 ggtaacgaga tatgataccg taactggtga ggaaattcca ttatctgagc gagttggttt
     5281 tgttgataga aaactgcaac ggattagtcg tgaggtttca gatgaagtca cattttataa
     5341 acttgtaaaa gatgcttatc atgcttcgta tggttatgag tatcaaggag attctctcct
     5401 tttggcgcgt gagaatcttt tggcaacttt tgaggactat tatctagcta agactggaaa
     5461 tcaaccaaca ttagagcaaa agaaagaaat tgcgactatt atctcttaca atgtctttca
     5521 gatggatgga ttgaaaaaaa gtagtcctta ttcagctaaa caaaagcaat ctcaacaatt
     5581 gagtttgttt gcggatgagc tagaagtcca agaagtagaa gaatctaaaa cccatatcaa
     5641 agactggaaa aagaatagaa tgataggttt tgagcgccta tctagcgagg aaagtgaaat
     5701 gaaatttgat gtcgtgatag gaaatcctcc gtatcaggaa acaggagaag ccagagatga
     5761 gccgatatat cactatttta ttgatgaagc ttataaaatc gcagataaat ctatactaat
     5821 caccccagca aggtttttat ttaaagctgg tcaaactcct aaaaattgga tggaaaagat
     5881 gcttgaagac aagcatttaa aagttcaatt ctatgaactt aaaagcggta aagtatttac
     5941 aggaacggat attaagggtg gagtagcaat aacttatcgt gatgttgata aagatttggg
     6001 accaatcgga gtatttacta tttttgaaac tttgaatggt attgtaaaaa aaatcgaaaa
     6061 acatccagaa tttgaaactt tttctgattt agtttatcct caaggtattt atagattttc
     6121 aaataaactt ttttctgatt atccaaatgc tgaaaagctg caaggaaaag ggacaaaaaa
     6181 taaaattgtt tcgaaatcac taactcagtt agattttgtt ttcaaaaaca acgaagaaaa
     6241 agaccatgtt gctctacttg gaagaattgg ctccgaacgt gtttatcgat atattaataa
     6301 aaaatattta gatttaccgg aaacattcga aaattataat gtttttgtac cagaagctaa
     6361 tggaagtggt gccttaggtg aagtcttatc aacaccccta atcggggaac ccctaatcgg
     6421 gcatacagat acttttttat ctattggtaa ttttaaaaca aaatttgaag ccgatgcttg
     6481 tattaaattt attaaaacta aattcgctag agtattatta ggtgttttga aagttactca
     6541 gcataattca cgcaaaactt ggtattacgt ccccctccaa gactttacgg tcaattcgga
     6601 cattgattgg acacaatcag tggctgatat tgacctccag cttgatcaaa aatatgacct
     6661 ttcccctgaa gaaattgcct ttattgagaa tcatgtaagg gagatggatt agaaaagtat
     6721 ttttatttga caaatagtgc tcaatgatct aaaatgacta tataggatta ggtcaggaag
     6781 catacgatgc cctgaccctt tttgtactta tgagatgaga aagtcatttg ttagataaat
     6841 tgacttcgtt agcaaacgtt caaaaaagga aaacttatgc cagtagaaat taaaaccact
     6901 aaagagattc atcctaaaat ctatgcctat accacaccga cagtaaccag taatgaaggc
     6961 tggattaaga ttgggtatac agaacgtgat gtcacacaac gtatcaagga gcaaacgcat
     7021 acagctcata tagctacaga tgtcttatgg actggtgatg cagcttatac agaagagcct
     7081 gataagggga aaactttcaa ggaccatgat ttccaccatt tcctttcttt ccatgatgta
     7141 gaacgtcgtc ccaagacgga atggttctat tttaatggaa ctcctgaaaa atcaaaaaat
     7201 ctttttgata agtttgttca gcatgatttg tctggttatc agcctggaaa aggacaggac
     7261 tatactctgc gacaagagca agaagaagca gttgctaaga cattagctta tttccaagaa
     7321 catgctggag gcaagtttct ctggaatgcc aagccacgct ttggtaaaac cttgtctacc
     7381 tatgacctag ctcgacggat ggaagctgtc aatgtcctaa ttgtaacaaa ccgccctgcc
     7441 attgctaact catggtatga tgattttgaa acattcatag caggtcaaac gacttacaag
     7501 tttgtttctg aatcagatag ccttaagagt cgtccaatct tgtcacgaca agaatttctt
     7561 ggtattttag ctgacgatgt aagacaactt gcttttatca gtctccaaga cttgaaagga
     7621 tctgtttatt taggtggaga gcacgataaa ctcaaatggg taactgatct gcattgggac
     7681 ttgttggtta ttgacgaggc tcatgaagga gttgatacct tcaagactga ccaagccttt
     7741 aataagattc gacggaattt tactctgcat ttgtcaggta catcatttaa agcattggct
     7801 aaaggagatt ttacagagga acaaatctac aactggtctt atgctgatga gcaggctgct
     7861 aagtattcgt ggtctcttga gcaagaagag gaaaatcctt atgaaagctt gcctcagttg
     7921 aatctcttta cctatcaaat gtctcagatg attggcgaaa agttagaaaa aggcgctcag
     7981 atcgatggtg aaaatattga ctatgttttt gacttaagtg aatttttcgc tacagatgat
     8041 aaagggaaat ttattcatga gcatgatgtc agaaattggt tagatactct atcaagcaat
     8101 gaaaaatatc cattttcaac caaagaactc cgtaatgaac tcaagcatac tttttggctt
     8161 ttagaacgtg tcgcttcggc caaagcatta aaagccctac tagaagaaca cccaatctat
     8221 gaaaactatg agatcgttct agctgctggt gacggacgta tgtccgaaga agacgataaa
     8281 gtcaaactca aatccttgga cttggttaga aaagcgatag cagagaatga caaaaccatt
     8341 accctatccg ttggtcagct gacgacaggt gtcactatcc ctgaatggac aggtgtattg
     8401 atgttatcaa atttgaaatc accagctctt tatatgcagg ccgccttccg tgctcaaaat
     8461 ccttactcat ggagcgataa caaaggaaat cactttcgca aagaaagagc ctatgtattt
     8521 gactttgcgc cggaaagaac cttgattctc tttgatgagt ttgccaacaa cttatcgctt
     8581 gtaactgcag ctggtagagg aacttcagct acacgcgaag aaaatattag agaattatta
     8641 aacttctttc caattattgc cgaagaccgt gctggtaaga tggttgaaat tgatgcaaag
     8701 gcagttctaa ccactcctcg ccagataaaa gctagagaag ttcttaaacg aggttttatg
     8761 tccaatctct tatttgataa tattagtggt attttccaag caagtcaaac agttttagat
     8821 attttaaatg agctgccagt tgaaaaggaa gggaaggtac aagatagttc tgatttatta
     8881 gatttttcag atgttacagt cgatgatgag ggaaatgcag tagtagacca tgaaattgta
     8941 gttaatcagc aaatgcgact ttttggtgaa aaagtttatg gacttggtga atctgttgct
     9001 gagttagtca caaaagatga ggaacgaact caaaaacagc tggtcaatga cttgagtaag
     9061 accgtttctt cagtgattgt agaggaattg aaagcagatt attctctaaa aacaagggaa
     9121 actgagcaaa ttaagaaaca aattacagca acacttgaga atgaaattcg aaaaaatgat
     9181 atcgaaagaa aaatttctga agctcatatc aagcaagagt tgcaacagca gctcaaagaa
     9241 gcaaatgata aagcgcaaaa agataagatt caagaagatt tagaaaaacg tttagaagaa
     9301 aataaactca ttcataaaga aaaactagaa caaacactca aaaaagaagt ggaaaaaatg
     9361 cctgagaaat ttatcgaaca ggttgagata aaacgtgtgg aacagttgaa acaatcagct
     9421 caagatgaaa ttcgtgacca tttacgaggg tttgcaagaa caattccaag ttttattatg
     9481 gcttacggtg atcaaactct aacacttgat aattttgatg cctttgttcc tgaacatgtt
     9541 ttttatgaag taacagggat tacgattgat cagtttagat atttgcgaga tggtgggcag
     9601 gattttgcag ggcatctctt tgataaagca acatttgacg aagctattca agaatttctt
     9661 cgcaagaaaa aggagttggc ggattatttt aaagatcaaa aagaagacat ttttgactat
     9721 attccaccgc agaagaccaa ccaaattttc actcctaaac gagtggtgaa aaggatggta
     9781 gatgatttgg aaaaggaaaa tccagggatt tttgatgatc catctaagac ttttattgat
     9841 ttatatatga agtcaggcct ctatattgca gaacttgtga agcggttata taatagcaat
     9901 ggcttgaaag aggcctttcc aaatcctgaa gaacgcttaa aacatatttt ggaaaagcaa
     9961 gtttatggat ttgctccgtc tgagattatc tataacattt ccactaattt tatatttggt
    10021 aatctttcta aagatatcag taggaagaat tttgttttag cagataccat tccagcggct
    10081 aaagaaggga gcattcaaaa gttggttgat tcctattttg aaaataatta aaaagaaggc
    10141 cgagtcaaaa ttctttgaaa tcagaaaaaa cgcataatat tgagtgcttt tgtactgccc
    10201 ccaaaaagtt agacagaaaa aatctaactt ttggggggca gttcagacaa tccttggtat
    10261 tatgcgtttt attgtgggaa gatgtataat ggattgaaat aagatatgaa caaatcgatt
    10321 aggaaaggaa aattgattta tagaaatgtt ttagtagtcg atgcgtactg ttatagattc
    10381 aacgaactat aaaatctgaa gaaagcaatt ttaaccagat cttagaagca gttgtgtact
    10441 actctaactt caatagacta tatcatatag ataaaaaaca actccctgat gattcaagga
    10501 gttgtctata gttaaattag tttttagaag cttcttggaa ttctggattt ttccatgctt
    10561 cgtcaatgat agcttgcaat tctttagcag atgcttgcat tttttgagtt tctgcatcgt
    10621 tcaatggaat gtttactgga cgaacgatac catgtgcacc aacaacagct ggttgaccga
    10681 taaagacatt ctcaactccg tattgacctt cttggaatac tgaaagtgga agtactgcgt
    10741 tttcatcgtc aaggattgct ttagtgatac gagcaagggc tactgcgata ccgtagtatg
    10801 ttgcaccttt tttgttgatg attgtgtagg ctgcatcacg aacaccttcg aacaattcaa
    10861 tcaattcagc ttcttgaaca ttttgagtgt ctttaaggaa ttcttcaagg tttacaccag
    10921 cgatgttagc gtgtgaccaa acagcgaact cagagtcacc gtgttcaccc atgatgtagg
    10981 cgtgcactga acgagcatcc acatccaatt tttcagcaag tgcttgacgg aaacgagctg
    11041 agtcaagtga agtacctgaa ccgataacgc gttctttagg gaaaccagag aatttccaag
    11101 ttgagtaagt caaaacgtca actgggttag cagcaacaag gaagatacct ttgaaaccag
    11161 attcaacaac ttgagttacg attgatttgt tgatagcaag gtttttacct acaaggtcaa
    11221 gacgagtttc acctggtttt tgaggtgcac ctgcagtgat cacaacaagg tcagcgtctg
    11281 cacagtcaga gtattgagct gcatagattt ttttaggtga agtgaaggca agggcgtgac
    11341 taaggtcaag cgcatcacca acagcttttt catgcaattg tggaatttcg ataattccaa
    11401 gctcttgtgc aattccttgg ttaacaagtg caaaagcgta agatgaacct acagcaccat
    11461 caccgacaag gataactttt ttgtgttgtt tagttgaagt cattgtttta aacatctcct
    11521 taattttatt aggggatttt ccctagacaa cttcattcta tcacttttaa aaaactttgt
    11581 cacgaatatg ccttatagtt ctcgatgtaa acgttttagt ggtttagagg ctgaaataga
    11641 tgggaattta tggtataatg ttgttactta ctaattgtga aatgaggcat ttattaatgc
    11701 aggataaaaa tttagtgaat gtcaatctga caaaggagat gaaggcaagt tttatcgact
    11761 acgccatgag tgttatcgta gcgcgagctc ttcctgatgt tcgagatggc ttaaaacctg
    11821 ttcaccgtcg cattctctac ggaatgaatg aattgggtgt gaccccagac aaaccccata
    11881 aaaaatctgc tcgtattaca ggggatgtca tgggtaaata tcacccacac ggggattcct
    11941 ctatttatga agccatggtc cgtatggctc aatggtggag ctaccgttac atgcttgtag
    12001 atggtcatgg gaattttggt tccatggatg gagatagtgc tgccgctcaa cgttataccg
    12061 aggcacgtat gagcaagatt gctctggaaa tgcttcgtga tatcaacaaa aatacagttg
    12121 atttcgttga taactatgat gccaatgaac gggaaccctt ggtcttgcca gcgcgttttc
    12181 caaacctttt ggttaatgga gcaactggta tcgcggttgg gatggcaacc aatattccac
    12241 ctcataatct gggtgaaacc attgatgcag tgaagttggt catggataat cctgaagtga
    12301 ctaccaagga cttgatggaa gtcttgcctg gaccagattt tccaactggt gctcttgtca
    12361 tggggaaatc aggtatccat aaggcttatg aaacaggtaa aggttcgatt gtcctacgtt
    12421 ctcgtacaga gattgaaacg actaagactg gtcgtgagcg tatcgttgta acagaatttc
    12481 cttacatggt caataaaacc aaggtgcatg agcatattgt tcgcttggtt caggaaaaac
    12541 gcattgaggg tatcacagca gtacgtgatg agtcaaaccg tgaaggtgtt cgatttgtta
    12601 ttgaagtcaa gcgcgacgcc tcagccaatg ttattctcaa taacctcttc aaaatgaccc
    12661 aaatgcaaac caattttggt ttcaatatgc tcgctatcca aaatggtata ccgaaaattt
    12721 tgtctcttcg tcagattttg gatgcttata tcgagcacca aaaagaagtg gttgttcgtc
    12781 gtacacgttt tgataaggaa aaagcggaag cgcgcgctca tatcttagaa ggtctcttga
    12841 ttgcgctaga ccatatcgac gaagtgattc gtatcatccg tgctagtgaa acggatgcgg
    12901 aagctcaagc tgagttgatg agcaagttta agctttctga acgtcaaagt caagctatcc
    12961 ttgatatgcg tcttcgtcgt ttgacaggtt tggaacgcga taagattcaa tctgagtatg
    13021 atgacctctt ggctctgatt gcggatttag cagatattct tgctaagcct gaacgtgttt
    13081 ctcaaattat caaagacgaa ttggatgaag ttaaacgtaa attttctgat aaacgccgta
    13141 cagagttgat ggttggacag atcttgagtc tcgaggatga ggacttgatt gaagaatcgg
    13201 atgtcttgat taccctttct aacagaggct acattaagcg tttggatcag gacgagttca
    13261 ctgctcaaaa acgtgggggt cgtggtgtcc aaggaacggg agtgaaagat gatgactttg
    13321 ttcgtgagtt agtgtcaact agcacccatg atcatctgct cttcttcaca aacaagggac
    13381 gtgtctatcg tcttaaaggt tatgaaattc ctgagtatgg tcggactgcc aaagggctac
    13441 cagtagtcaa tctcttgaaa ttggatgaag atgaaagtat tcagacggtt atcaatgttg
    13501 agtctgatcg cagtgatgat gcttatctct tctttacaac ccgtcacggt attgtgaaga
    13561 gaactagtgt taaggagttt gccaatattc gtcaaaatgg tctcaaagcg ctgaatttaa
    13621 aggatgaaga tgagttaatc aatgtcttgt tgacagaagg agatatggat attatcattg
    13681 gtaccaagtt tggttatgca gttcgcttta atcaatcagc cgttcgtggt atgagccgta
    13741 tcgccactgg tgtgaaaggt gttaaccttc gtgaaggaga cacagttgtt ggtgccagct
    13801 tgattactga tcaagatgag gttcttatta tcacagaaaa aggatatggt aagcgtacag
    13861 tcgctactga atacccaaca aaaggtcgtg gtggtaaggg aatgcagaca gctaaaatta
    13921 ccgaaaaaaa tggcttgctg gccggtctta tgactgttca aggtgatgag gatttgatga
    13981 ttatcactga tacaggtgtc atgattcgaa ccaatcttgc caatatttca caaacaggac
    14041 gtgcaactat gggagttaaa gtaatgcgcc tggatcaaga tgctcagata gtgactttca
    14101 caacggttgc ggtggcagaa aaagaagaag ttgggacaga aaacgaaaca gaaggtgaag
    14161 cataatgtct caaaaaaata ataaaaagaa aaacaagcga aaaaatctgc tgacaaatat
    14221 cctagcagga tttctgatat tactgtcact ggctttgatt tttaatactc aaattcgaaa
    14281 tattttcata gtctggaata ccaataagta tcaagttagc caggtatcaa aagaaaaatt
    14341 agaagaaaat caggatacag aaggcaattt tgactttgat tctgtcaaag ctatctcttc
    14401 ggaagctgtt ctaacttctc aatgggatgc tcaaaaatta ccagttattg ggggaattgc
    14461 aattcctgaa ttggaaatga atttgccgat ttttaaagga cttgataatg ttaatctctt
    14521 ctacggagct ggtacaatga aacgcgagca agtaatggga gaaggaaatt atagtctagc
    14581 tagtcaccat atctttggtg ttgataatgc taataaaatg ttattttctc ctttagataa
    14641 tgctaaaaat ggcatgaaga tttatctaac cgataaaaat aaagtttata cttatgaaat
    14701 acgtgaagtc aaacgtgtga caccggatcg tgttgatgaa gttgatgata gagatggggt
    14761 caatgaaatc acattagtaa cctgtgaaga ccttgctgct acagaacgta ttattgtcaa
    14821 aggtgatttg aaagaaacaa aagattattc acaaacatct gatgaaatcc taacagcttt
    14881 caatcaacca tataaacaat tttattaata caaatcagtg aaatcatgga tttcactgat
    14941 tttttaagat tttcataaaa atata
//
LOCUS       DAVMMR010000049        14268 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMMR010000049 DAVMMR010000000
VERSION     DAVMMR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.049"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1288)
                     /locus_tag="VYS74_001544"
     CDS             complement(<1..1288)
                     /locus_tag="VYS74_001544"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV3789555.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2601)
                     /locus_tag="VYS74_001545"
     CDS             complement(1366..2601)
                     /locus_tag="VYS74_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV3789556.1"
                     /translation="MKLLFRNLAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVWLLAL
                     SVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFLTIYR
                     NLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQF
                     CVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYV
                     SGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLTWMLF
                     VGLSLLAIFLTILNLKNDI"
     gene            complement(2792..3643)
                     /gene="rgg"
                     /locus_tag="VYS74_001546"
     CDS             complement(2792..3643)
                     /gene="rgg"
                     /locus_tag="VYS74_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV3789557.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKGNETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3767..4108
                     /locus_tag="VYS74_001547"
     CDS             3767..4108
                     /locus_tag="VYS74_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3789558.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4362..6065)
                     /gene="ilvD"
                     /locus_tag="VYS74_001548"
     CDS             complement(4362..6065)
                     /gene="ilvD"
                     /locus_tag="VYS74_001548"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV3789559.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6298..7230)
                     /locus_tag="VYS74_001549"
     CDS             complement(6298..7230)
                     /locus_tag="VYS74_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV3789560.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7227..8084)
                     /locus_tag="VYS74_001550"
     CDS             complement(7227..8084)
                     /locus_tag="VYS74_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3789561.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8088..9434)
                     /locus_tag="VYS74_001551"
     CDS             complement(8088..9434)
                     /locus_tag="VYS74_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3789562.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            complement(9447..9731)
                     /locus_tag="VYS74_001552"
     CDS             complement(9447..9731)
                     /locus_tag="VYS74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3789563.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9735..11765)
                     /locus_tag="VYS74_001553"
     CDS             complement(9735..11765)
                     /locus_tag="VYS74_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV3789564.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            11956..12963
                     /locus_tag="VYS74_001554"
     CDS             11956..12963
                     /locus_tag="VYS74_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3789565.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            12966..13166
                     /locus_tag="VYS74_001555"
     CDS             12966..13166
                     /locus_tag="VYS74_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3789566.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13318..13500
                     /gene="rpmF"
                     /locus_tag="VYS74_001556"
     CDS             13318..13500
                     /gene="rpmF"
                     /locus_tag="VYS74_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV3789567.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13516..13665
                     /gene="rpmG"
                     /locus_tag="VYS74_001557"
     CDS             13516..13665
                     /gene="rpmG"
                     /locus_tag="VYS74_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3789568.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14144..>14268)
                     /locus_tag="VYS74_001558"
     CDS             complement(14144..>14268)
                     /locus_tag="VYS74_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3789569.1"
                     /translation="ATGWFTVSGQWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         4613 a         3016 c         2453 g         4186 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgagtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccacaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aagaatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaaataa gaaaccaaac
      421 catcgtaacg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgcctc cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatttggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatatagta gaacttgctt ggcttttgca cttctggggc gaagagttta ttttccacat
     1021 aggaacggac aacgggcgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttatag cgcttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ttcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccacaaa taaagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacaggg taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtcgcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgattg ctactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgccc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg acagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatgcgcaa gccacctgca aaatcactga tgacattgat
     2281 caaacacagg ctagaaaagg caaagagact agcttgctga acaactaggg ctgctagaaa
     2341 aaatagaacc gcctgaaaca aaccgctata gaccatccat ttgaccttgt ccctcgtgta
     2401 atctgcccga atccctacaa aaactgtaaa gagggtcgga agaatcatga caatattcgc
     2461 catagcaaca gcaaaagatg cttgtgacaa ggtcgatgca tagacgataa agaccaggtt
     2521 gaaaatcgaa gcaccaaaag cattgaagaa gcgtgataaa gtcaaaattc tataagctag
     2581 atttctaaac aatagtttca tagataatct cccttcttgt tctaagttct acatcaagaa
     2641 tagctttagt atagcactta tgaatttgcg aaacgattct tttgaatata tgcaacaaat
     2701 aaaatgagca aaaaaacgat agaacttttg taatgacctg agagagggta acagttctat
     2761 cgcttcaaat tttttcttgg tttgcagata ctcaacattc ggtcgtattt ctatagtatt
     2821 cggcagattt attacagcca agcatctcaa aaatacggac agcatcctcc atctttttct
     2881 ggccttcctt gactctacct tgcttgctat caaggagacc ttctgcccac agataaacaa
     2941 ttcggaaata ggtctcattt cccttgtaga aatgctcttc gataacacgt ttaaaataat
     3001 aggcattggt aaattcttca cactcaatac tagctaaaaa gccattcaat agtatagtat
     3061 gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc ggatttacgt accatttctc
     3121 gtacatatct agtaaaaaga gaaacagata aaaatggaga actgactgaa aataaattga
     3181 gttcatagat tccccagatc tcggtagaaa acaaataatc atgaaggact tttccttcct
     3241 ctgctgttaa gtctaccctt tcatctatgc tcttcatata agacttgata ataatggcat
     3301 ttagaatatg tttctgtttg ttgtgagaat gggcatgctt ttgatactcc ctgcgatata
     3361 agtcctcaag aggtgctata ttctttggtt ccaagacatc tgtaatttct tttctcaact
     3421 cagaatctgt atcatactgg aaacctctcg ccagaaagag gatctcctcc acactggcag
     3481 atatattttc cagagcaaat agaaactttt ccaccgaaag ctcactctga cctgtttcaa
     3541 aacgggacaa catagacggc gaaaattgtc ctccggttgc ttgtctcagt gagatatttc
     3601 ttgattctcg taattgtcta aagacttttc caatctgctc catagacttc cccttgattc
     3661 cgtattttct tcattttatc atatttttca gaaaattcat caaaaacttg ccaaattgtc
     3721 agaattatga gaaaatagag gatatttatc acgtggaggg actgctatga gagacgatat
     3781 caaaatcaat gaccgtgctt tggccttgca agaccaaatc atcgaaaaac tagagaaagt
     3841 ttttgataca gatgtggaat tggatgttta caatctaggt ctgatttatg aaatcaatct
     3901 ggatgaaacg gggctctgca agattgtcat gaccttcacc gatactgcct gtgattgcgc
     3961 cgaaagcctg cctattgaaa tcgtggcagg tctgaaacaa atcgagggta tcaaagatat
     4021 caaggttgaa gttacctggt cgcctgcttg gaaaatcaca cgaatcagtc gctatggccg
     4081 tattgccctt ggactaccac ctcgttaagc aggccaatca cttttaaaga tgaaaaaagc
     4141 aagccaaaaa tggcttgctt tttgatactc aatgaaaatc aaagggcaaa ctagaaaact
     4201 agccgcaggt tgctcaaaac actgttttga agttatggat agaactgacg aagtcagctc
     4261 aaaatactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata cctacggcaa
     4321 ggcgacgttg acgtgatttg aagagatttt cgagtatgag tttatttttt acctgacttg
     4381 tccatattcc agaagtctgt cacggctccg cgtgaagcag atgatacgat gtgggcatat
     4441 ttaccgagga caccacggct gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca
     4501 agttcttctt cggatacggc catggaaatt tctttggtat cttggtcaac cgtaacgata
     4561 tcgccggtac ggagataggc aattggtcca ccatcctgag cttcaggagc gatatgtcca
     4621 acaaccagac cataagtacc accagagaaa cgtccgtccg tcaagagggc caccttatct
     4681 ccctgacctt taccaacaat cattgaagaa agtgacagca tctcaggcat accaggacca
     4741 cctttaggtc caacaaaacg aacaacgact acatcgccat caacgatttc atctgtcaga
     4801 acggcctgaa tcgcatcttc ttctgagtca aagaccttag ctggcccaac gtgacgacgc
     4861 actttaacac ctgatacctt ggcaactgca ccgtcaggag caaggttccc gttcaagatg
     4921 ataagcggac catccgcacg ttttggattt tcaagtggca tgataacttt ttggcctgga
     4981 gtcaagtctg caaagtcagc caagttttca gctacagtct taccagtaca tgtgatgcga
     5041 tctccgtgaa ggaaaccatt tgccaacaag tacttcataa ccgcagggac accaccgact
     5101 tcgtagaggt cttggaagac atactgacca gatggtttca agtcggccaa gtgaggcaca
     5161 cgttcttgaa tcgtattgaa gtcctcaagt gacaagtcaa catttgcggc atgggcaatg
     5221 gcgagcaagt gaagagtggc gtttgtagaa ccaccgagag ccatcgttac agtaatagca
     5281 tcttcaaagg cttcacgagt caagatatct gatggtttga gaccaagttc caacatctta
     5341 acaacagcac gtcctgctgc ttcgatatct tctttcttat cagctgattc agctgggtga
     5401 gaggatgacc ctggcaaact catccctaga acttcgatag cagttgccat ggtattagca
     5461 gtatacatac caccacagcc accagggcca gggcaggcat tacattcaag acgtttcacg
     5521 tcctcagctg tcatgtcacc gtggttccat tttccgatac cttcaaagac agaaaccaag
     5581 tcgatatctt taccatcaag atttcccggt gcaatagttc caccataggc gaaaatagct
     5641 gggatatcca tattagcaat agcaatcata gatccaggca tgttcttgtc acagccaccg
     5701 atagcgacaa aggcatccac gttgtgacca ctcatagccg cctcgatgga gtccgcgatg
     5761 atgtcacgag atgttagaga gaaacgcata ccaggcgttc ccatagcgat cccgtctgct
     5821 acggtaatgg ttccaaactg tacaggccaa gcgcctgcag atttgacacc ttctttagcc
     5881 agtttcccga aatcatgcaa gtgaatgtta catggtgtat tttccgccca agtcgaaatc
     5941 actcccacaa tcggtgtttc aaagtcctta tctgtcatac cagtcgcacg aagcatagca
     6001 cggttaggtg atttaaccat gctgtcataa atgctactgc ggtgacgttt atctaattca
     6061 gtcatcttat ccctcccatt tcagttttta ctattatagc acaattttcg catgaagaac
     6121 agaataaaaa tcttgaattt tcagaaaatt ctatacacat gtgaaatatt taaaattaaa
     6181 aacaacaaag cggattagtg cactttctga tgaccagaat atgcttttta atccgctttc
     6241 tttaaataac gtactgtaat ttttacagaa attctttcag ataagtgtat ttaacatcta
     6301 tcttgcatta taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc
     6361 attaagaaga taatccattt tccctacttg accgaatctt tcttgaacac ccatccgatg
     6421 aatttttgtc attccatcat cagagaataa ttcacataaa gaactgccaa ttccacctat
     6481 ctgattgtgg ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc
     6541 tggtatcggt ttgattctaa ataaatctat cacacttact gaataaccta atttagacag
     6601 ttcatctgca actcgaatac ttggagcaac cattatacca gaagcaacga ttacaagatc
     6661 ttcaccatgc cttaactcaa tgtagccttt agaaaaatct tctccacctt gatacacagg
     6721 aactggagct tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt
     6781 caatatttca cgaaattgga tatcatcagt tgcttcaaaa atgatcgatt taggaattaa
     6841 acgtaacaat ccaatttctt caaatggcat atgtgtccca ccattcattt ctgcagttac
     6901 tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa
     6961 ttgatcaaat actcttcgtg aagcaaaagg accaaatgta tgaagataag gtctaaaccc
     7021 ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc caacattcac
     7081 ataacggtct ccaaaatcct tttcaagatt attagtagcc atcgaacttg acaaatcggc
     7141 ttctaagact actatatcag aataactttg attagcctct aaaaggaagt ctctatatac
     7201 atgccgtaat tctttcgtgc ttctcatcat tctgtttcct ccaattcctg acttaatctt
     7261 tctacaactg aagttaacat ttgtctctcc tctacagtag ggcgaagatg atggttggat
     7321 ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta
     7381 ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct
     7441 gaacccttga ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct
     7501 ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca
     7561 attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca
     7621 tttaactcac catctccaac aatagcataa gtataaaagg gactctttct tattctctga
     7681 ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct
     7741 atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa
     7801 gaatataaga attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt
     7861 cctccgtgac cttttgataa tatgaaataa tctctatctc gtgctgcaaa tatttctgga
     7921 gtcattggca ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct
     7981 ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagtc
     8041 gcaaattttc ttaactcatc ttccctattt ttacttaaaa tcatccctta ttcctccttt
     8101 gcagatggct ttgtaataaa ggatactcca aacataactg ctagaataag aacaagacca
     8161 atcacaatgc ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga
     8221 ccaaaatctg catctgagaa agttgatcct tggaaaccaa gtcctcccaa aactggcatt
     8281 aaaaagactg gaagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca
     8341 cgaacaccac cagatgcatt cccaatgaca cctgcagtcg ctccacagaa gaaatgaggc
     8401 acaacacctg gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa
     8461 ccaccaacaa aactagagat aaatccaatt agaactgcat tgggtgcata agtataaaca
     8521 atcggacaat ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag
     8581 gctggaacaa tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct
     8641 gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct
     8701 ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatggat
     8761 aaagtgatac taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc
     8821 tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc
     8881 aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt
     8941 tgaacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagata
     9001 agaggcaagc tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat
     9061 agagtgtggt gccctgttaa aaaaatatat ttaaatcgag taaaacgagc gattaagata
     9121 ttgaacacca tgcctgcaaa cataatcagt gcagtagctg agccatatgt tgttaaagct
     9181 acagctacaa ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac
     9241 atggtaccaa atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag
     9301 aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga
     9361 actaatccta aaattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat
     9421 atgaacttca tcataacgct agcctcctat ataagtcctt tttcttcaca aagtttagta
     9481 attaattctc gtagttcatc catatcaata atactattta agatacgaac atctccaaga
     9541 tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct
     9601 gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca
     9661 gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca
     9721 atttttaaca ttatctaatc ctcctgttta attatcattt taatgtcatc atagtttttt
     9781 gatgatatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa
     9841 gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt
     9901 tctggattat ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca
     9961 ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa
    10021 ggcccaaact cttctacttt ttgaatcatt gcctcagggt agttctcagt tatcttatct
    10081 tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa
    10141 ctaacctgat aggtttcttt ggtaatgagt tgttctagca ctggtacaat ttcctttcta
    10201 tcattttttt ggtaaagata attctttaac gccaatctta attccaattc ttgtgtaata
    10261 attccatatc ttttgacaat attcaggatt tgttcaatct caaaatcccc atactctaaa
    10321 ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata
    10381 accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca
    10441 tagtcactac tagctttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt
    10501 tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac accattagga
    10561 caaatgattg ctgctttata gcatttttga ggcaaagtat ctgctttctt taaataacct
    10621 ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc
    10681 atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca
    10741 tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta agccggtata
    10801 aggtggcggc gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata
    10861 tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct
    10921 acttcacctt caaagcaact tgataataaa acggtgatat agcgataatc atcctcagaa
    10981 aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct
    11041 tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac
    11101 ctacacagaa tgaatactaa accgaaaagg caaactttta attgattaac aataggtact
    11161 agctgtagct tctcataata atctttaact acctgattta tcaaatcata agttaatgaa
    11221 tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac
    11281 tcactaacca tttgaaaagc taagcggtgc ttattccact ctgaaccgtg taaagtataa
    11341 ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat
    11401 tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc
    11461 gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt
    11521 ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat
    11581 aaactatagc gaccttgttt tttatccagc actatccctt tattagctag ataaggcact
    11641 aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca
    11701 tataatgata gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca
    11761 aacatttctc ttcctttcta actacaatta tagtgtaaaa attataagaa taaaagagta
    11821 tttgagcaca atataatctc accttttaaa agaaattatt gggctgaaac tttttagtga
    11881 ttattttcaa ctttctattg acttttaaaa acaaagtgat atcatttaga tatcaaataa
    11941 taggaggtca ttcttatgac tgaaaaactc atcaattcaa aaccaaatgg tgtattcgca
    12001 ttgattctca ttgaattgac aatcgtactt ggtatcttta tatttataat gggtgttggt
    12061 tcggaaaaca tttttggaat tattatcgga cctttactaa tcgtaattgc aggtctagct
    12121 catgctggtt taaaagttgt caaacctcag gaagctctgg ttctgacact ctttggtaat
    12181 tatacaggta ccatcaaaga acctggtttt tactttgtca atcccttcag cgtagcagtc
    12241 aaccctgcaa accacactcg acttggacaa agtggtgatg ttagcacaaa atctcctttt
    12301 ttaggagcta aatcatcaaa tgacaatgat gtaaatcttg aaattggcaa gaaacagatt
    12361 tccctcaaag tcatgacctt gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac
    12421 cctgtagaaa tcggtatcgc ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc
    12481 aacgttgata actacaaaga atatctttca ttgcaatgtg atagcgccct ccgtaatatt
    12541 gtccgcatct atccttacga tgtgtctcct aatgtggata ctacgggtga cgggcaagca
    12601 gatgaaggta gtctccgtgg ctctagcgaa attgttgcta accgtattcg tgaagaaatc
    12661 caaagtcgtg ttgaggatgc tggcttggaa atccttgaag cacgtatcac ttacctagct
    12721 tatgctccag aaattgctgc cgttatgctt caacgccaac aagcatctgc cattattgat
    12781 gcacgtaaga tgattgtaga tggtgctgta ggaatggttg aaatggcact agaacggctc
    12841 aatgaagggg aattggtaga acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc
    12901 cttgtcgttc tttgtggcaa tcatgatgca caaccaattg tcaacacagg aagtctttac
    12961 taaagatggc tgaagctaaa aaaaagcaga tcccccttcg actctcaaca aagttatacg
    13021 ctgcactcgc atcatgggca gaagacgact ttcgttcagt caatgggcaa atagaatatc
    13081 tcctcacaga gtgtgtcaag caacggaaaa aagacggaaa atacgtatca gaaaccattg
    13141 acgaaccatt tgagattgat atttaacacc ttctcctgtc tgccgtgtcg gataggagaa
    13201 ttttttcatc tttttttgtc aagtaacttt actttacaaa aaaaatgtgt tatcctagta
    13261 tggttgatga aaatcagtag attgaatcga atataaatac ctaaaggaga aatcaaaatg
    13321 gcagtacctg cacgtcgcac ttcaaaagcg aagaaaaaca aacgtcgtac acactacaaa
    13381 gtaacagctc catctgtaaa ctttgacgaa actactggag attactcacg ttctcaccgt
    13441 gtatcactta aaggatacta caaaggacgt aaaatcgcta aagctgcatc agctgaataa
    13501 tagaagggag atactatgcg cgtaaatatt acacttgaac acaaagaatc tggtgaacgc
    13561 ttgtacctta cttctaaaaa caaacgtaac actccagacc gccttcaatt gaagaaatac
    13621 tcaccaaaac ttcgcaaaca cgttgtgttt acagaagtga agtaagcatt caatacgaat
    13681 caatacagaa gaaaaacgct tgatcagcgt tttttctttt tgttaatttc actatattgc
    13741 aagggaaatc attcgaatga ctacattatt gaaataagaa tacggattcg gacaattaaa
    13801 agcaagagga actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc
    13861 tctacactat ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga
    13921 atatgtaagt gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat
    13981 acaaaagttg accaactaga tttctgaata tccttatata tccattctta aaattggttt
    14041 aaatagcgta gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag
    14101 aagatttttt agttcagaat gattagattt agctaatgga tacctatcct acccactcac
    14161 cgttagcatt gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat
    14221 tataggtata gtaccattga ccagaaactg taaaccaacc agtagcca
//
LOCUS       DAVMMR010000050        14094 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMMR010000050 DAVMMR010000000
VERSION     DAVMMR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.050"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            83..880
                     /locus_tag="VYS74_001559"
     CDS             83..880
                     /locus_tag="VYS74_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV3789570.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VYS74_001560"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VYS74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VYS74_001561"
     CDS             2383..3657
                     /locus_tag="VYS74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV3789571.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEHYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VYS74_001562"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VYS74_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV3789572.1"
                     /translation="MNGIINLKKEAGITSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4806..5108
                     /locus_tag="VYS74_001563"
     CDS             4806..5108
                     /locus_tag="VYS74_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3789573.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5216..5854
                     /gene="udk"
                     /locus_tag="VYS74_001564"
     CDS             5216..5854
                     /gene="udk"
                     /locus_tag="VYS74_001564"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV3789574.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5982..7322
                     /locus_tag="VYS74_001565"
     CDS             5982..7322
                     /locus_tag="VYS74_001565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV3789575.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7300..7512
                     /locus_tag="VYS74_001566"
     CDS             7300..7512
                     /locus_tag="VYS74_001566"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV3789576.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7509..8384
                     /locus_tag="VYS74_001567"
     CDS             7509..8384
                     /locus_tag="VYS74_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV3789577.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8377..9192
                     /locus_tag="VYS74_001568"
     CDS             8377..9192
                     /locus_tag="VYS74_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV3789578.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            9185..9616
                     /locus_tag="VYS74_001569"
     CDS             9185..9616
                     /locus_tag="VYS74_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3789579.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9623..11290
                     /gene="recN"
                     /locus_tag="VYS74_001570"
     CDS             9623..11290
                     /gene="recN"
                     /locus_tag="VYS74_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV3789580.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11292..12020
                     /locus_tag="VYS74_001571"
     CDS             11292..12020
                     /locus_tag="VYS74_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV3789581.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12099..13922
                     /gene="lepA"
                     /locus_tag="VYS74_001572"
     CDS             12099..13922
                     /gene="lepA"
                     /locus_tag="VYS74_001572"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV3789582.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4174 a         2565 c         3351 g         4004 t
ORIGIN      
        1 acttttatga aatatagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg actcgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg agcatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgaga gtccaagctg
     1741 ccacaatttg agctatactc atgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ttaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttattat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgttaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt caaagctcaa caatccacaa aagccatcgg ggaaagtttg
     2941 gaacactatg cggaaagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaataacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt aatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgctcgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gatgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataata attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct attaggtact
     4681 caaataatct atatagaaat ttagaggtgt gaaatgaagc aatttaaaat tctttcagat
     4741 aaatatttag agtccattac aggttctggt gggaacttag gcccaggatt tggtgtgata
     4801 attccatgat gcgaaatgag tttcgagaaa gggtggagca acttcttcaa caaaaagaaa
     4861 taaatgaaaa tagtgagttg agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa
     4921 atgaaaaata ccaaacggtc atggccaatt tgagtcaagg gttgtcactt tacctcatga
     4981 cgcatcatta ccaggcacct aagtctgtca ttgattttgg tttatggatt gccaaagctc
     5041 ctagccagga aagagggaga ctggctttct tgcagatgct tgctcaaacc ctacagggct
     5101 ttcgttaaaa ctgtcgaaca tcagagggaa gactctggtg ttttttgctg atgagactat
     5161 aaattttgta aaaaatgtga tagaatagac gacggataaa ataacggagg atagcatgca
     5221 aaatagacca atcattattg gagtgacagg tggttctggt ggtggtaaaa ctagtgtttc
     5281 aagagccatt ttatcgcatt ttcctgatga aaagatttcc atgattgagc atgattcata
     5341 ctacaaggat cagtctcatt tgacctttga agagcgtgtc aaaaccaact atgaccatcc
     5401 ttttgccttt gatacagact tgatgatcga gcagattaag gaattgttgg cagggcgtcc
     5461 ggtggacatc ccgacctacg actatacaga gcatacacgg agtagcaaga cctatcgtca
     5521 ggagcctcaa gatgtcttta tcgttgaggg cattttggtc ttggaggaca agcgtctgcg
     5581 cgatttgatg gatatcaaga tttttgtgga tacggatgac gatgtgcgca ttattcgtcg
     5641 tatcaagcgt gatatggagg agcgtggccg tagccttgat agcgttatta accagtactt
     5701 aggtgtggtc aaaccaatgt accaccagtt tatcgagtca actaagcgtt atgctgatat
     5761 cgtcattcct gaaggggtta gcaataccgt ggctatcgac ctgttgacga ccaagattgc
     5821 aaagattttg gaagaagctc gaaacagcaa ataatcacat gtggaggcct tgcctccttt
     5881 ttctattttt ccctcataat ggtacataaa tggagatttt taggcatatt tttggtataa
     5941 taatacccat gaaagagcaa gtaaatgaat agtaggtgga gatggaaaag tatttatcgg
     6001 taacaacttt gaccaagtat ctgaaaatga aattcgataa agacccatac ttggaacggg
     6061 tctatttaac tggtcaagtt tccaactttc gtaaacgacc tactcaccaa tatttctccc
     6121 taaaggatga ccatgcagtt attcaagcga ccatctggtc tgggatttat cagaaattag
     6181 ggtttgacct ggaagaagga atgaagatca atgtgattgg gcgtgtacag gtctatgaac
     6241 caagtggtag ctactccatc atcattgaaa aagctgagcc tgatggggtt ggggcgcttg
     6301 cgattcagtt tgaacaactt aagaaaaaat tgacagaaga aggcctgttt caagaacgct
     6361 tcaagcaagc tctaccccaa ttttctaaga gaattggggt agtaaccagc cgtagtggag
     6421 ccgttattcg agatattatc acgaccgtca gcaggcgatt cccaggtgtt gacatccttc
     6481 tttatcctac caaggttcaa ggtgaagggg ctgcggagga aattgctaga aatattgcgc
     6541 gtgctaatca acgggacgat ttggatttgc tcattattgg tcgtggaggt ggttctattg
     6601 aggatctctg ggcctttaac gaagaaattg tggtacgagc tatttttgaa tctcgtttgc
     6661 cagttatctc tagtgtgggg catgagacgg atgtgacctt ggcagatttt gtggcagatc
     6721 gacgcgctgc aacgccaaca gcggcggctg aactggcaac acctgtgacc aagttggatg
     6781 tattaactca tttgcaaaat caggaaaaac ggatggcaac ggcagtccga aatgttctat
     6841 ctaagaaaca agaggctttg aaaaaatgca gtcagtctgt tatctttaga caacctgagc
     6901 gcttgtatga cggttatttg caacgcttgg accaactgca actgcgtttg aaacaaagtt
     6961 tgcgaactcg gatttctgat aacaaacaat tagttcaagc aagaactcat caattagtac
     7021 aattatcacc tgttaccaaa atccaacgct atcaagaccg tttaggacag ttggacaagc
     7081 tcttaggtag ccaaatggcg ttagtttatg acgccaaggt tgctgaggcc aagcgacttt
     7141 cggaagcttt gctcatgttg gatactagcc gaatcgtggc gcgtggttat gctattgtca
     7201 aaaaagaaga gtccattgta gattcggttg agagtttgaa gaaaaaagac caagtaacgc
     7261 ttttgatgcg agatggtcaa gtagaattag aggttaaaga tgtcaaaaca aaagaaattt
     7321 gaggaaaatc tagcagaact ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct
     7381 ctggaagatg cgattactgc ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct
     7441 acgctggaca aggctgaaaa gaccttggtc aaggtcatgc aagaagacgg aacagaaagt
     7501 gattttgaat gaaaaagcaa gaaaaattag ctcttgtcga gtcggctttg gaagattttt
     7561 atggagacca gcagtttgcc tctagtttac gggagtctgt tctctattct attcatgctg
     7621 gtggcaagcg tattcggcct tttctcttgt tagaagttct ggaagccttg caggttacca
     7681 tcaaacctgc tcacgcgcag gtagctactg ccttggagat gattcataca gggagcttga
     7741 ttcacgatga ccttcctgct atggatgatg acgattatcg aagagggcgg ttaaccaatc
     7801 acaagaaatt cggtgaagct atggccattt tggctggaga tgccttattc ttagacccat
     7861 atgccttgat tgcgcaggca gatttgccaa gtcagattaa ggtggacttg attgccaact
     7921 tatcccttgc ttcaggtagt ctgggtatgg tggcagggca agttttggat atggagggcg
     7981 aacaccagca cttgtctttg gaagaacttc agattattca tgccaataag actgggaagt
     8041 tactagccta tcccttccaa gcggcagcta ttatagctga attgtcacct gaaatgcagg
     8101 tgaagctgaa aactgtgggt gaattgattg gacttgcttt tcaagtcaga gatgatgtac
     8161 tggatgtgac agctagtttt gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag
     8221 aaaaatcaac ctatcctgcc ctgttgggct tggaagagtc cattgccttt tgtaaccaaa
     8281 ccctggatca agctaatgaa aaattagaag aaattgccca gcagcttccc tttgaaacag
     8341 aatcgattgt aagtgtagta gaaagtttga gaatcaatgg ctaaggaaag agtggatgta
     8401 ctagcttata aacaggggtt gtttgaaacg agagagcagg ccaagcgagg tgtaatggct
     8461 ggcctagtcg tagcagtcct taatggagaa cggtttgaca agccaggaga gaaaattcca
     8521 gatgacaccg aattaaaact caagggagag aaactcaagt atgtcagccg tggtggtttg
     8581 aaactggaaa aggccttgca ggtctttgat ttgtcggtgg atggcgcgac tacgattgat
     8641 atcggggcct ctactggagg ttttaccgat gtcatgctac agaatagtgc caagttggtc
     8701 tttgcagtcg atgttggtac caatcagttg gcttggaaat tacgccaaga cccacgagtt
     8761 gttagcatgg agcagttcaa tttccgctat gctgaaaaga ctgatttcga gcaggagccg
     8821 agctttgcca gtattgatgt gagtttcatt tcccttagtc tgattttgcc agccttgcat
     8881 cgtgtcttgg ctgatcaagg tcaggtggta gcacttgtca aacctcagtt tgaggcagga
     8941 cgtgagcaga ttgggaaaaa tggaattatt cgagatgcta agattcatca gaatgtcctt
     9001 gaatctgtaa cagctatggc agtagaggca ggtttttcag tccttggctt ggacttttct
     9061 cccatccaag gtggacatgg aaatattgaa tttttagcgt atttgaaaaa agaaaagtca
     9121 gcaagcaatc agatttttgc tgagattaaa gaagcagtag agagggcgca tagtcaattt
     9181 aaaaatgaat aaaaaagaga gacttgaaaa aattagaaga ttggtgacag attatcaaat
     9241 cggcacgcaa gaagaaattg tagaacattt gaaagaagca ggtatcactg ccactcaggc
     9301 gacggtatcc cgagatatca aagagttagg tattgtcaaa attcctttga gagacaacac
     9361 ctatgtctac gaattaccaa aatcaatcgt aaaaagtctg caactggctg aagacaatat
     9421 cgaatcggct gaattgatgg ataagatgat caatctccaa gttattccag gaaatacggc
     9481 ttttgtaaaa gctcagttaa tcgatacttt tgcagacaag atttttagtt gtttgactga
     9541 tgatagctcg attttagtca ttgccagaag tggaagtcta gcagaggaaa tctttgaaca
     9601 agtaaaaaat tggtaggtct atatgttact tgaaatttcg ataaaaaact ttgccattat
     9661 tgaggctatt tccctcaatt ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc
     9721 agggaagtcg attatcattg atgccatgaa tatgatgttg ggagctcgtg cgacgacaga
     9781 tgttattcgt catggtgcac caaaggcaga gattgagggg cttttctcag ttgagaatag
     9841 tcgtctttta caggaaattt ttgatgagca aggtttagaa ttaggtgatg aaattatcat
     9901 ccgtcgggaa atcttgcaaa atggtcgtag tatcagccgt gtaaatggtc agatggttaa
     9961 tctgtctgtt ttgcgagcta ttgggcaaca tcttgtagat attcatggtc agcatgacca
    10021 tgaggagtta atgcgtcccc aactgcatat ccagatgttg gatgaatttg gtgatgccgc
    10081 tttttgggac ttgaaagaaa cctatcaaac gagttttgat gcctatcgga aaatgcgcaa
    10141 gcaggttctg gaagtcaaga aaaaccaaca ggaacacaag gaacgtatcg aaatgttgga
    10201 atttcaaatg gcagagattg aggcagcaaa cttgcaggct ggagaagact tggctctcaa
    10261 tcaagagcga gataaactcc tcaaccacaa aaatattgcg gatacactga ccaatgccta
    10321 cagtatgttg gacaatgaag atttttcaag tctggccaat gttcgttcag ctatgaatga
    10381 catggaaagt gtcgaagagt atgatcctga ataccgtgaa atttcaagct ctctgtctga
    10441 gacctactat gttttagaag acattagcaa acgtttggaa gctatcattg aggaccttga
    10501 ttttgatggc aatcgcctga tgcaggttga gaatcgtttg gacctccttc atactattac
    10561 tcgtaagtat ggtgggactg ttgatgatgt tttgctttat tttgccaaga ttacggaaga
    10621 atacaatctc ttgacaggca ataatctttc gtctgaggac atggaagcag agcttaagaa
    10681 gttggaagtc aatcttgtca atttggcagg tcaacttgct tctgctcgtc ataatttggc
    10741 tcagcaactc gaagctgaga ttaaacaaga actgcaagat ctttatatgg aaaaagccca
    10801 gtttcaggtt cattttagta agggaaaatt cagtcgtggg ggaaatgaaa tggttgagtt
    10861 ttatatttca accaaccctg gagaagactt taaacccttg gttaaggttg cttctggagg
    10921 ggaattatct cgtctcatgt tagccattaa gtctgccttt tcacgtaaag aaggcaagac
    10981 tagcattgtc tttgatgagg tggatacggg agtttcaggt cgtgttgctc aagctattgc
    11041 tcagaaaatt cataaaattg gtcagcatgg tcaggttttg gctatctccc atttgccaca
    11101 agtaattgcg attgcagatt atcaattctt tattgagaag attagtaatg accattcaac
    11161 ggtttcgact gttcgtctct tgacagtcga agagcgagtg gaggaagttg ccaagatgtt
    11221 ggcaggtgat gatgtgacag aagcagccct gacgcaagcc agagaattgt tgagaaacag
    11281 ggagaaataa gatgacagat tattatgtaa ttggagatgt tcacggaaaa gctgggatgc
    11341 tggaagacct tcttaaaacc tgggatggtc agacccagtt gctctttcta ggtgatttga
    11401 ttgacagagg tgaggatagt caccgtgttc ttgaaatggt caaggacttg gtggacaatc
    11461 aaggggctat ctgtttgtct ggaaaccacg agtatatgtt tttgacttgg ctagatgacc
    11521 cagaagaaag ttatgaccat tatcgtcgca atggtggaga tacaaccatt aactctatcc
    11581 taggtcgtcc cttggatgca ccagttgatg gagtagaaga tgccaagcgg gttgccgctg
    11641 aagcgtcaga cttggtcgaa ttcattcgtc aaatgccatt tgtagtagag acagacaagt
    11701 atatatttgt tcacgcaggt attgatttga ctttggacga ctggcatgaa acaacagatt
    11761 ataaaaaagt atggcttaga aaaccattcc acggagctga aaatcatact ggaaaaacca
    11821 ttgtcttcgg tcatacaccg gtctatggtt tgttacagca agaacgcggt acatctgagc
    11881 tttggactac agaagatggc aagattggaa tggatggagg agctgtttat ggtggtgtcc
    11941 ttcacgggat tgtctttaca gaccaaggaa tgacagaaca ccactttatc gaaaatgacg
    12001 gctttgtcgc tgaagattag tactcctagc agggtatggt cttgtcaaaa tatcaaaaac
    12061 aatttataat aaatagatac cctgaaagga agagaatcat gaacttagaa gaattgaaaa
    12121 aacgacaggg gaagatccgt aacttctcta ttatcgccca tattgaccac gggaaatcaa
    12181 ctctagcaga ccgcattttg gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc
    12241 agcttttgga tagcatggat ctagagcggg aacgtggaat tactattaag ttgaatgcca
    12301 tcgagttgaa ttacactgca aaagatgggg aaacttatat tttccacttg attgacacac
    12361 cagggcacgt tgactttacc tatgaagttt cacgttcgct agctgcctgt gagggtgcta
    12421 ttttggtggt cgatgcggct caaggaattg aggctcaaac tcttgccaac gtttatctgg
    12481 ctttggataa tgatttggaa atcatgccta tcattaataa aattgacctg ccggctgcag
    12541 atccggagag cgtgcgtaca gagattgaag atgtgattgg tttggatgcc agtgaagcag
    12601 ttttggcttc tgccaaggct ggtattggga ttgaagaaat cctcgagcaa attgtagaaa
    12661 aagtaccagc accaacgggt gatgtgacgg cgccacttaa ggccttgatt ttcgactctg
    12721 tttacgatgc ttaccgtggg gttatcctcc aagtgcgtgt catggacgga gtggtcaaac
    12781 ctggtgataa gattcagctc atgagcaata gtaagacctt tgatgtggcc gaagtcggta
    12841 tttttacacc aaaagcggtt ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg
    12901 cggcgtctat caagacggtt caggatactc gtgtgggtga taccgttacc ttggcaacca
    12961 atcctgcggc agaaccatta catggttata agcagatgaa tcctatggtc tttgcgggtc
    13021 tctacccaat cgaatcaaac aagtacaatg acctgcgtga agcgcttgaa aaattgcaac
    13081 tgaatgatgc tagtcttcag tttgaaccag aaacatctca ggcacttgga tttggtttcc
    13141 gttgtggatt tcttggactt ctccatatgg atgttatcca ggaacgttta gagcgtgagt
    13201 tcaatattga tctcatcatg acagctccgt ctgttattta caaagttaat ttgaccgacg
    13261 gtgagtctat ggatgtgtct aacccatctg agtttccaga cccaactaag attgcgacca
    13321 ttgaagagcc ttatgtcaag gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa
    13381 tggagctagc tcagcgtaag cgtggggact ttgtgactat ggactatatt gatgataacc
    13441 gtgtcaatgt tatctatcaa attcctcttg ctgaaatcgt ctttgacttc tttgataaac
    13501 ttaagtcttc gacacgtggt tatgcaagtt ttgactacga attgtcagaa tatcgcccat
    13561 ctaagctagt gaaaatggat atccttctca acggagacaa ggtggatgcc ctcagcttta
    13621 tcgttcataa ggactttgct tatgaacgtg ggaaactcat cgttgataag ctcaagaaaa
    13681 tcatccctcg ccaacaattt gaggttccaa tccaagcggc tattggacac aagattgtcg
    13741 ctcgtactga tatcaaggcc cttcgtaaga acgtacttgc taaatgttat ggtggtgacg
    13801 tttctcgtaa gcgtaaactc cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca
    13861 tcggatcagt tgaagttccg caagaagcct tcctcagcgt cttgagtatg gatgaagaat
    13921 aagttatcag aaatgccttt ccttgttcac aggaaattta tataagaatc aatagtaaag
    13981 caggcttaga tggtctgctt ttttagtttt tatactcttc gaaaatctct tcaaaccacg
    14041 tcagcgtcgc cttaccgtat gtatggttac tgacttcgtc agtttcatct acaa
//
LOCUS       DAVMMR010000051        13705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMMR010000051 DAVMMR010000000
VERSION     DAVMMR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.051"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            1..670
                     /locus_tag="VYS74_001573"
                     /pseudo
     CDS             1..670
                     /locus_tag="VYS74_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(877..3135)
                     /locus_tag="VYS74_001574"
     CDS             complement(877..3135)
                     /locus_tag="VYS74_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3789583.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRSSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGATREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3364..3594)
                     /locus_tag="VYS74_001575"
     CDS             complement(3364..3594)
                     /locus_tag="VYS74_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV3789584.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3784..4467
                     /locus_tag="VYS74_001576"
     CDS             3784..4467
                     /locus_tag="VYS74_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3789585.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4467..5201
                     /locus_tag="VYS74_001577"
     CDS             4467..5201
                     /locus_tag="VYS74_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3789586.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVIENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5355..6212
                     /locus_tag="VYS74_001578"
     CDS             5355..6212
                     /locus_tag="VYS74_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV3789587.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6349..7170
                     /locus_tag="VYS74_001579"
     CDS             6349..7170
                     /locus_tag="VYS74_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV3789588.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            7184..8056
                     /locus_tag="VYS74_001580"
     CDS             7184..8056
                     /locus_tag="VYS74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV3789589.1"
                     /translation="MKVIDQALLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            8338..9021
                     /gene="rpiA"
                     /locus_tag="VYS74_001581"
     CDS             8338..9021
                     /gene="rpiA"
                     /locus_tag="VYS74_001581"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV3789590.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            9035..10246
                     /locus_tag="VYS74_001582"
     CDS             9035..10246
                     /locus_tag="VYS74_001582"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV3789591.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            10248..10790
                     /locus_tag="VYS74_001583"
     CDS             10248..10790
                     /locus_tag="VYS74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV3789592.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            10806..11615
                     /locus_tag="VYS74_001584"
     CDS             10806..11615
                     /locus_tag="VYS74_001584"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3789593.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRTYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            12091..13071
                     /locus_tag="VYS74_001585"
     CDS             12091..13071
                     /locus_tag="VYS74_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV3789594.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            13096..13608
                     /locus_tag="VYS74_001586"
     CDS             13096..13608
                     /locus_tag="VYS74_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV3789595.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         4091 a         2467 c         3062 g         4085 t
ORIGIN      
        1 gttgatagag atagacttaa aaactatctt actgacaatc cagatgctta tttgactgaa
       61 atagcttctg aatttggctg tcatccaact accatccact atgcgctcaa agctatgggc
      121 tacactcgaa aaaaagaacc acacctacta tgaacaagac ccagaaaaag tagccttatt
      181 tcttaagaat tttaatagat taaagcacct agcacctgtt tagattgatg aaacaggatt
      241 cgatacttat ttttatcgag aatatggtcg ctcattaaaa ggtcagttaa taagaggcaa
      301 agtatctgga agaagatatc agaggatttc tttggttgca ggtctaacaa atggtgaatt
      361 aatcgctcca atgacttacg aagagacgat gacgagcgac ttttttgaag cttggtttca
      421 gaattttctc ttaccaacat taaccacacc atcggttatt attatggata atgtaagatt
      481 ccatagaatg gggaagctag aacttttatg cgaagagttt gggcataaac ttttacctct
      541 tcctccctac ttacctgagt acaatcctat tgagaaaaca tgggctcata tcaaaaagca
      601 cctcaaaagg gtattaccaa gttgcaatac cttttacgag gctcttttgt cctgttcttg
      661 tttcaattga ctatagcaga ttgaaataag atgagaacaa atcgattggg aaagtaaaat
      721 taatttctat aaatgtttta gcaattgttt cgtactattt cagattcagt ctactatata
      781 tcataatgga caaagggcaa gctgtcaaga aattacagtt attttagtaa aaaaagaagc
      841 agaaaaattt cattttctac tccttttcta taggatttat ttttcttttt cagaactttt
      901 aacttcagct tttttggcag atttaatctg aatgtttccc ttgctagtca taactgcttt
      961 gagatctttt tcgcttggat tttcaaggta gtagtcagtg attgtgtcct caatatagtc
     1021 ttgaatagtc cgacgaagtg ggcgtgctcc catttttgga tcataaccta ggtcaaccaa
     1081 cttttccttg accttgtcag ttacatccaa acgaatgttg ttgctagaga ggcgcttgtt
     1141 aacatctgct agcatgagct cgacaatctg aaggaggtta tccttgctga gagccttaaa
     1201 ttcgataatg ccatcaaaac ggttcataaa ctctgggcta aagaagttac cgagttcacc
     1261 gaggacagaa ttggtacgtc cttctctagt agcaccaaat ccaacgctag cttcggtctt
     1321 tcctgtacct gcatttgagg tcatgataat gatggcatcc ttgaagctaa cggtgcgtcc
     1381 ttgcccgtct gtcaaacgac catcgtccaa gacttgaaga aacatgtgca taacatctgg
     1441 gtgagctttt tccacttcat cgagaaggat gagagaatat ggattgtggc gaactttttc
     1501 agttaattga ccagcctcat catagccaac ataacctgga ggggcgccga ccaacttagc
     1561 cacactatgt ttttccatgt attcactcat atcaaagcga atcatactat cagcagaacc
     1621 aaaaagttcg atagccagtt gtttggaaag ttctgtctta ccgacaccag ttggcccaac
     1681 gaagaggaag cttccgattg ggcggttagg ggtaccaagt ccgacacgat tacggcgaat
     1741 agccttggca atcttatcga ctgcatcatc ttggccaata acatgagact tgagatcttc
     1801 ggctagatgg atgagttgag attgttcttt ctctttcaaa tcaccaacag ggatattggt
     1861 tttctgctcg ataatgtgct caatagtttt ctcgctgatg ataggagtat cctggtctgt
     1921 gatctttttc ttttgcattt ccttatactt ggcaatctgg tcgcggaagt aggccgcctt
     1981 ctcaaaatct tcttctcgtg tagcttgaga cttgagattt tcagcctcaa tcaagcgctg
     2041 atcaattact ttaggatcca caaaattcaa ggtcaagttc atcttagaac cagcttcatc
     2101 taggaggtca atggccttgt caggcaagaa gcgatcttgg atgtagcgat tggaaagagt
     2161 tgcagctgct tcaatcgcag catctgtata ttgaacgtgg tggtaatctt cgtatttctt
     2221 ttgaatccct ttgagaatag tgattgtttc gtccaccgtt ggttcatcga ctttaacagg
     2281 ctgcatacga cgctcgaggg cagcatcctt ttcaatgata cggtattcat tgagggtagt
     2341 agcaccgact agttgcagtt ctccacgagc aagggctggc ttgaggatat ttcctgcgtc
     2401 catattacca tcactcgcag aaccagcacc aacaatttca tggatttcgt cgataaagag
     2461 gatgatgtct tcacgtttgc gaatttcttc catgagtttt tgcatgcgtt cttcaaattg
     2521 tcctcgaatc cccgttcctt gaactaagct aaccacatcc agacggatga cttgtttacc
     2581 ttggagttta tgtggcacat cgccatcgac aattttctga gctagacctt cgacaacggc
     2641 cgtttttccg acaccaggtt caccgataag gacaggatta ttcttggttc tacgattgag
     2701 aatctcgatg acacggataa tctcatcgtc gcgcccaata acggggtcaa tgtctccacg
     2761 acgggcaatt tcagttacat taataccgaa ttcttccagc aggccttttt cttggctagg
     2821 tggcggagtt tgagcagatc cacgattttg ggaaccataa ccgccgtttc caccgtaacc
     2881 tccacctgat tgggttgggg gaataggagg agtattgcta gaagatctga aattgtttag
     2941 atcattgaag aaatcaccaa agggatcgaa gtcacgattg ttcagatccg tcataccttt
     3001 gaagaggcta ttgttaggat ctgttttgat aatcttatag cagttttgac agaggtcaat
     3061 ttgtttttgt tttccattga gattggtgta aagatgaatt gttgagtcgt taattttaca
     3121 gttttgacaa agcatacatc tacctcattt ctttctttag cctgacttgt ttaaaggtca
     3181 aaaatagtca aattctatac tctcattata acaggattgg ggtgtaaagc aagtaaaaag
     3241 attgaagctt tgagaagttg ttacaatgaa aatttttgtg aatttggact ataaaaaaat
     3301 cctatttgtg agacaaaaag gattttactt agacatacag ggacaatccc agcttgtttt
     3361 agattagtag agaagttcgt aaatttccat tgcgattaag tcaatgctat caaacgtata
     3421 cgtttcgcga tcttctacgt cacgaagggt aaagattggg ccgttttctt cgtcgtggct
     3481 gtacgcaact tctgcaacaa caactccttc gcgttcaaaa ttacgtttta aatttccgcc
     3541 atcgtttgcc attgcttcaa ggcggttgat gattctaacc aaatgtgatt ccattttgat
     3601 tctccttcat ttcttatcgt tactatttta ccataaagga ggccaacttg actagaaaaa
     3661 actaagattt ctcgctaatt ctgctagttt aaagattttt aaataaattg gataaaaaat
     3721 ttaaatttta attcaaaaca tgttataatc atacagtatt ctattttaga aagcagtgtg
     3781 actatgaatt tttctttttt acctaagtat ttaccttatt ttaactatgg ggctgttgtg
     3841 acgattctta tttctatctg tgttatcttt ttgggaacta ttttgggtgt tgtcttggct
     3901 tttgggcaac gttcaaagtt taaaccgctt gtttggttgg ccaacttgta cgtttggatt
     3961 ttccgtggga caccgatgat ggttcaaatt atgattgcct ttgctcttat gcatatcaat
     4021 gctccgacta ttcagattgg aattttaggt gttgattttt cgcgtctgat tccagggatt
     4081 ttgattatct ctatgaatag tggtgcttat gtttcggaga ctgttcgtgc cggaatcaat
     4141 gcggttccaa aaggtcagct agaggcggct tattcgctag ggattcgtcc taaaaatgcg
     4201 atgcgttatg tgattttgcc acaagcagtc aaaaatatct tgccagcatt ggggaacgaa
     4261 tttatcacca ttatcaagga cagctccctc ttatcagcta ttggggtcat ggagttgtgg
     4321 aatggggcta caacagtttc tacaacaacc tatctacctt taacaccact tttatttgca
     4381 gcattttact acttgattat gacctctatt ctgacagtag ccttgaaagc ttttgaaaaa
     4441 catatgggac aaggagataa gaaataatga cagaaacctt gataaaaatt gaaaatttac
     4501 ataaatcttt tggaaagaat gaagtattga agggcatcaa cctcgagatt aaaagaggag
     4561 aagttgtcgt tatcatcggt ccttcaggga gtgggaaatc taccttgctt cgctctatga
     4621 atttgttgga ggaagcaacc aaggggaagg ttatctttga gggagtcgat attacggaca
     4681 agaagaatga cctgtttgcc atgcgtgaga agatgggcat ggttttccaa caatttaacc
     4741 tctttcctaa tatgactgtg attgaaaata ttaccttgtc acctatcaag accaaaggtg
     4801 aaagcaggga agttgctgag aagagagccc aagagctttt ggaaaaagtt ggtttgccag
     4861 ataaggcaga cgcttatcca cagagtctgt cgggtgggca gcaacaacgg attgccatcg
     4921 cgcgtgggtt ggctatggaa ccagatgttt tgctctttga cgaaccaact tcagccttgg
     4981 atcctgaaat ggtaggtgag gtattggctg ttatgcaaga ccttgccaag tcagggatga
     5041 ctatggttat cgtaacacat gagatgggat ttgcccgtga ggtggcagat cgtgttatct
     5101 ttatggcaga cggtgtggtt gttgaagatg ggacacctga gcagattttt gaacaaaccc
     5161 aagaacaacg gactaaagat ttcttgagta aggttttata atctatttta aaatttcaag
     5221 acaagattag tgaaaagttc aaccagagct ttttcttata gtttgaaact ataggataac
     5281 ctaggaaaga agtgttagag ggcactagca gtttttggta taatggaaga tatttgaaag
     5341 aaaagagaag tgatatgaca cagattattg atgggaaagc tttagcggcc aaattgcagg
     5401 ggcagttggc tgaaaagact gcaaaattaa aggaagaaac agctctagtg cctggtttgg
     5461 tagtgatttt ggttggggat aatccagcca gccaagtcta cgttcgcaac aaggagaggt
     5521 cagctcttgc ggctggttcc cgtagcgaag tagtgcgagt tccagagacc attactcaag
     5581 aggaattgtt agacctgatt gctaaataca atcaggatcc agcttggcat gggattttgg
     5641 ttcagttgcc attaccaaaa cacattgatg aagaggcggt tctattggct attgacccag
     5701 aaaaggatgt ggatggtttc catcctctaa atatgggacg cctttggtct ggacatccag
     5761 tcatgattcc atcaacacct gcaggaatta tggaaatgtt tcatgaatat gggattgaat
     5821 tagaaggtaa aaatgcggtt gtcatcggtc gttctaatat cgttggaaaa cccatggctc
     5881 agcttctttt ggccaagaat gcaacagtga ctttgaccca ctcacgcacc cataatcttg
     5941 ctaaggtggc tgctaaagca gatattctgg tggtcgcaat cggtcgtgcc aagtttgtga
     6001 ctgctgactt tgtcaaacca ggtgcggtag tcattgacgt tgggatgaat cgtgatgaaa
     6061 atggtaagct ctgtggggat gtggattatg aggcagttgc cccacttgct agctatatta
     6121 cgccagtccc tggaggtgtc ggccctatga ccattactat gctgatggag caaacttatc
     6181 aagcagcact tcggacattg gatagaaaat aagagaaaaa tcgctgaaga gagtgtattt
     6241 tctatagcta tatttaaaat ggcagaaatg aatattaaat tttagatata agattacaaa
     6301 aggaggtctg cgcctccttt ttgttgtata atggagtgag gtgattagat gattttaaaa
     6361 atttataata gggaatatag tttacaatgg gatggaatat actacctagc actaattgat
     6421 tatccaaata ttcaagagtg ggaattagaa aaaattgcta aatttatagc ttacgaaaaa
     6481 cttcataaac gtcaaacaag tattgagtgt gctgattctt gtttaaaaaa agaaatttta
     6541 gattacatct gtcagcatcc ctttctgcca ccatttaccc ctacagataa aagagtagcc
     6601 tcgacttatg atctacataa gaggttagtg acttcagact actgtagtca tactacgact
     6661 atagatgcag cgatttctat ctttaaaaca ggtcagctct tatctgctgt gaaagccttt
     6721 aggcgagatg ctgaagagtt ggttttggat agtagaaatg ctgcatcgga tccgatagat
     6781 tattttgatt atgtcatgtt agggtggtca aatacaagtt ctggttatcg attagcgatg
     6841 gagcgtttat taggtcgagc tccttcagag aaagaattac aagacaagtt tattcctgga
     6901 gtgagttttc attttatcta tacagatttg attaaagtta ctggttatat ttttgatggc
     6961 taccatgttg caaaaattaa ggacatgttg aatttattta gtgagttgta tatttgtatt
     7021 attccaactc ataataagag ccaatttgaa aatattattc caactaaaat acaagatagg
     7081 gtgcattatc ttgactatgc tggagaagac ttagaagagt ggactaagaa agtctatcaa
     7141 gttgttttaa aacaatcaga taaaggatag ttgaggaaga acgatgaaag tgattgatca
     7201 agccttacta gaaaaagtta ttattgaacg ttctcgttca agtcataaag gagattatgg
     7261 tcgtctgctg ctgcttggtg gtacttatcc ttatggaggt gccatcatca tggctgcttt
     7321 agcagctgta aaaagcggtg caggattggt gaccgttgga acggacaggg aaaatatccc
     7381 ggctctgcac agccatttac ctgagcctat ggccttttct cttcaagacc agcaattgtt
     7441 aaaagagcaa ttggagaagg cagaagttgt cttgctgggg cctggtttac gagacgatgc
     7501 ttctggagaa aatctagtaa aacaggtctt tgttaattta agccaaaatc agattttgat
     7561 tgtagacgga ggtgccttga ccattcttgc taggacaagt ttgtcatttc cctcaagcca
     7621 gcttatccta actcctcacc aaaaagaatg ggaaaaatta tctggaatta cgattgaaaa
     7681 gcaaaaggaa gatgcaacgg ctagtgttct gacttccttc cctcaaggaa caattttggt
     7741 ggagaaaggc ccagctactc gtatttggga agttggccaa tctgattatt accagttaca
     7801 ggttggtggt ccttatcagg cgactggggg aatgggagat actctggctg ggatgatcgc
     7861 aggatttgta ggccaattcc gacaggccag tctctacgag cgtgtggcag tagcgaccca
     7921 tcttcattca gccatagccc aagaactatc tcaagaaaat tatgtggtct taccgacgga
     7981 aatcagcagg tatcttccca aaataatgaa aattatttgt caacaagaga gggtaagcaa
     8041 agataaactt gtttaaaata gacagataaa tctacttcct tctcttaatt tatgattctt
     8101 gaaatgcgcc tgctttaagg ctaaactaga cgtatcaaag ttaagagttt gtttttgtaa
     8161 ctggaaagag atgaacaaat caaatacata aaagataaac tcatttttat tcgtttggta
     8221 aaaataaaat gcatattttt aaagaaagat tgatgaaaaa tgtagatttg atgtttttac
     8281 ttgaataaaa agcggtttca tagtaaaata agaactgaga atgatggagg taaatcggtg
     8341 gaaaatctga agaaaatggc aggtatcaag gctgctgagt tcgtgagcga tggaatggtc
     8401 gttggacttg gaacaggctc gactgcctat tattttgtcg aagaaatcgg tcgtcgtatc
     8461 aaggaagaag gattgcagat tacagctgta acaacttcta gtgtgaccac taaacaggca
     8521 gaaggcctta atatcccgct caagtctatt gaccaagtag attttgtcga tgtgacggtt
     8581 gatggcgcgg atgaagtgga tagtcagttt aatggaatca aaggcggtgg tggtgccctt
     8641 ctcatggaaa aggtggtcgc aacaccatca aaagaataca tttgggtggt ggatgaaagc
     8701 aagctggtcg aaaaactagg tgcttttaaa ttgccagtag aagtggttca gtatggtgca
     8761 gagcaggtct ttcgtcattt tgaacgagct ggctacaaac caagtttccg tgaaaaagac
     8821 ggccaacgtt ttgtgacgga tatgcagaat tttatcattg acctcgcctt ggatgtcatt
     8881 gaaaatccaa ttgcttttgg acaagaattg gaccatgtcg ttggtgttgt ggagcatggt
     8941 ttattcaacc aaatggtgga taaggtaatc gttgctggac gagatggagt tcagatttca
     9001 acttcaaaaa aaggaaaata gaagggggca taagatgtct aaatttaatc gtattcattt
     9061 ggtggtactg gattctgtag gaatcggtgc agcaccagat gctaataact ttgtcaatgc
     9121 aggggttcca gatggagctt ctgacacact gggacacatt tcaaaaacag ttggtttgaa
     9181 tgtcccaaac atggctaaaa taggtcttgg aaatattcct cgtgaaactc ctcttaagac
     9241 tgtagcagct gaaagcaatc caactggata tgcaacaaaa ttagaggaag tatctcttgg
     9301 taaggatact atgactggac actgggaaat catgggactc aacattactg agcctttcga
     9361 tactttctgg aacggattcc cagaagaaat cctgacaaaa atcgaagaat tctcaggacg
     9421 caaggttatt cgtgaagcca acaaacctta ttcaggaacg gctgttatcg atgattttgg
     9481 accacgtcag atggaaactg gagagttgat tatctatact tcagctgacc ctgttttgca
     9541 gattgctgcc cacgaagaca ttattccttt ggatgaattg taccgtatct gtgaatacgc
     9601 tcgttcgatt acccttgagc gtcctgccct tcttggtcgc atcattgctc gcccttatgt
     9661 aggtgaacca ggtaacttca ctcgtacggc aaaccgtcgt gacttggctg tatctccatt
     9721 ttccccaact gttttggata aattgaatga ggctggtatc gatacttatg ctgtgggtaa
     9781 aatcaacgat atctttaacg gtgctggtat caaccatgac atgggtcaca acaagtcaaa
     9841 tagtcatgga attgatacac tattgaagac tatgggactt gctgagtttg aaaaaggatt
     9901 ctcattcaca aacctagttg actttgatgc cctttacggc catcgtcgta atgctcacgg
     9961 ttaccgtgat tgcttgcatg agtttgatga acgcttacct gaaattatcg cagctatgag
    10021 agagaatgac cttctcttga ttactgcgga ccatggaaat gacccaacgt atgcaggaac
    10081 ggatcacact cgggaatata ttccattgtt ggcctatagc cctgccttta aaggaaatgg
    10141 tctcattcca gtaggacatt ttgcagatat ttcagcgact gttgccgata actttggtgt
    10201 ggaaactgct atgattgggg aaagtttctt agataaattg gtataagatg acgcgctatg
    10261 ctttgctggt gagaggtatc aatgttggtg gtaagaataa ggtcgtcatg gcggagcttc
    10321 gtcaagaatt gacaaacttg ggactggaaa aggttgagag ctacatcaat agtggcaata
    10381 ttttctttac ttcgatagat tccaaagccc aattggttga aaagctagag actttctttg
    10441 cagtccatta tccatttatt cagagctttt ctttactgag tctagaggac tttgaggcgg
    10501 aacttgaaaa tctaccagct tggtggagca gagacttggc acgaaaagat tttctctttt
    10561 acactgaggg tttggatgtg gaccaagtca tcgcgacagt tgaaagttta gagctgaaag
    10621 atgaagtgct ttattttgga aaacttggga ttttctgggg gaaattttct gaagaatcct
    10681 attctaagac tgcctatcat aagtacttgc tgaaggtgcc tttctaccgc cacattacta
    10741 ttcgtaatgc taaaaccttt gacaaaattg gtcaaatgct aaaaaaataa taaaggagac
    10801 acacaatgac atttttaaac aaaatccatg aaactgctac tttcctgaaa gaaaagggaa
    10861 ttgcagcccc tgagttcggt ctaatccttg gatcaggact tggagaattg gcagaagaaa
    10921 tcgaaaatcc agttgtagta gactatgctg agattccaaa ctggggccgt tcaacagtag
    10981 tcggtcatgc tggtaaattg gtatatggtg aactggcagg tcgcaaggtc ttggctcttc
    11041 aaggtcgttt ccatttctat gaagggaatc ctctggaagt ggtgactttc ccagttcgtg
    11101 tgatgaaagt tcttggatgt gaaggtgtta ttgtaaccaa tgcagctggc ggtatcggat
    11161 ttggtcctgg taccttgatg gctatctcag accatatcaa catgacgggg caaaatccat
    11221 tgatgggtga aaacttggat gactttggcc cacgtttccc agatatgtct aggacctaca
    11281 caccagaata ccgtgccact gcccatgaag tggctaaaaa acttaatatc aagcttgatg
    11341 aaggtgtcta tatcggagtt actggtccga cttatgaaac accagcagaa attcgttcct
    11401 ataagacact gggagcagat gcagttggta tgtctacggt tcctgaagtt atcgtggcag
    11461 cccactctgg cttgaaagtt ctgggaattt catgtatcac taactttgcg gctggtttcc
    11521 aagaagaact caatcacgaa gaagttgtag aagtgactga acgtgttaaa ggtgatttca
    11581 aaggcttgct taaagcgatt cttgctgaat tgtaagaaaa aagatttaaa agggggagtg
    11641 cctctgtctt ttcaggattg actgcctatc cggattaaag aagaaacaga ggaatactat
    11701 gagcttcttc ctgctcttat aactgaaaga agcggaagaa taggtatgtc tgatctgata
    11761 gccagcattg tgaaagacaa gattctagga tactagcatt agcttcctag ccaagtagac
    11821 tagtatgata aggagagatg agaatgaatt gactttctga atttctcagt cttatcatat
    11881 atagcacaat gagatttcgc ttgagtctgc ttgtaaataa acgaaaagaa agataagaaa
    11941 taatgaaaat tggtcaacga attatgcgct ttggcataaa aaattaagta tcggagttgt
    12001 atctgttgta gtcggctttg atttctagct ccagctggaa tttcagccaa tgaagtaaag
    12061 caagatgtaa catctgaagt ggtaataggt gtgctagatt ctaaggagga attgaaagag
    12121 tcagaaaatg atgctccaaa actagaaact cctcttagag aggagccaag actagctcct
    12181 caaacgcttc cggaagcaag tgaagttctt gaaaacaaaa gggaagagtc aaaagtagag
    12241 ataacagagc cagctcaagc ggatgatatc cgcaaggttg ttggggaatt agccaaggat
    12301 ataagtatta ctaagttgta tatgacaggt cattctcttg gatgctacct agctcagatt
    12361 gcagaggttg aagcttacca aaaatatcct gatttttata accatgtatt gaggaaagtg
    12421 acaactttca gtgctcctaa agtgattact tccagaactg tttggaatgc taagaatggt
    12481 ttctgggatg ttggtttgga aagtcgtaaa ttagctgtta gtggaaaaat taagcattat
    12541 gtggttgata atgacaatgt tgtgactccc ttgattcata atgatcgtga tattgttaca
    12601 tttacaggta attcacgctt taaacaccgt tctcgtggct attttgaaag tccaatgaat
    12661 gatattccta actttaatat tggtaaacaa gctaccttgg ataaacatgg ttatcgtgat
    12721 ccgaaattgg ataaagtgcg attctttaag aaacaggctc tgcctcgatc ttctagtcaa
    12781 ccaagcgctg aaccaatgga aaatattgcc ttaggaaaac aggttactca aagttcgaca
    12841 gctttcggag gagatgctag aagagctgtg gatggcaaag tcgatggtaa ctatggtcac
    12901 aattctgtca ctcatacaaa cttccaatct aagccttggt ggcaagtaga tttggctaaa
    12961 gaagaaacca ttcgccaaat caatatttac aaccgaacag acactgccca ggatagattg
    13021 gcaaactttg atgtcattct tttagacagt tctggtaaag aaattgagtg aaaacgtata
    13081 acatctccta aagatgtgtc agcacaaatt acgattaacc ataaaaaagc gcgctatgtt
    13141 cggattgagc tagaaggcta taatgccctc agtcttgcag aagttgaagt tttccgcttt
    13201 atagctacga atggtgaaac ggcgatacaa gtttctaagt cagttcaacc aatcagtcag
    13261 actcctgtga aggataaaac attgacaatt caacacagtg gagcttacat tgcccgctac
    13321 tccataactt gggaagaagt tccagtagat aaagatggaa accaagttgt tcgtagtcat
    13381 tcttgggaag gaaacggtcg caaccagact gcaggttttg tcctcaacct cccaatcaaa
    13441 gaaaatatga gaaatctgcg agttaagatt gagaaaaaga cgggcctact atggaataga
    13501 tggcaaacaa tctatgaaaa cagaccaatt ttagctcaac cccaccgtaa aattacccat
    13561 tggggtacga cattgaattc caaggtgagt gacgatgatg tcttgtaatc tgatggtaca
    13621 atgatagtta gtttgtctag tttataagaa agtactacct gagcttgaat aggactcagg
    13681 tagctctctg tgaaagaaca aaatt
//
LOCUS       DAVMMR010000052        13422 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMMR010000052 DAVMMR010000000
VERSION     DAVMMR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.052"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            167..823
                     /locus_tag="VYS74_001587"
     CDS             167..823
                     /locus_tag="VYS74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV3789596.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            813..1022
                     /locus_tag="VYS74_001588"
     CDS             813..1022
                     /locus_tag="VYS74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV3789597.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            1240..2070
                     /locus_tag="VYS74_001589"
     CDS             1240..2070
                     /locus_tag="VYS74_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3789598.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2224..3078
                     /locus_tag="VYS74_001590"
     CDS             2224..3078
                     /locus_tag="VYS74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV3789599.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3178..4551
                     /locus_tag="VYS74_001591"
     CDS             3178..4551
                     /locus_tag="VYS74_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV3789600.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            4544..5605
                     /locus_tag="VYS74_001592"
     CDS             4544..5605
                     /locus_tag="VYS74_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV3789601.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5607..6299
                     /locus_tag="VYS74_001593"
     CDS             5607..6299
                     /locus_tag="VYS74_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789602.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6328..6897)
                     /locus_tag="VYS74_001594"
     CDS             complement(6328..6897)
                     /locus_tag="VYS74_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3789603.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7032..7646
                     /locus_tag="VYS74_001595"
     CDS             7032..7646
                     /locus_tag="VYS74_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3789604.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7648..9294
                     /locus_tag="VYS74_001596"
     CDS             7648..9294
                     /locus_tag="VYS74_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3789605.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9306..10592
                     /locus_tag="VYS74_001597"
     CDS             9306..10592
                     /locus_tag="VYS74_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3789606.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10644..11177
                     /locus_tag="VYS74_001598"
     CDS             10644..11177
                     /locus_tag="VYS74_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789607.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11255..11725
                     /gene="nrdI"
                     /locus_tag="VYS74_001599"
     CDS             11255..11725
                     /gene="nrdI"
                     /locus_tag="VYS74_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV3789608.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12018..13280)
                     /locus_tag="VYS74_001600"
     CDS             complement(12018..13280)
                     /locus_tag="VYS74_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV3789609.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3931 a         2498 c         3085 g         3908 t
ORIGIN      
        1 gtgttttgag ctgacttcat cagttctatc tgcaacctca aaacggtgtt ttgagcaacc
       61 tgcggctagt ttcctagttt gctctttgat tttcattgag tattattaag acaatatgag
      121 gagaaagatg aaagaaaaag gattttagga gggggcgcag gcagccatgc caacggctct
      181 tggctatgtc agtatcggcc tggcctgtgg aattatcggt gcgccctatg tgacacctgt
      241 tgagatgggc ttgatgagtc tctttgttta tgctgggagt gcccagtttg ccatgttggc
      301 actgattgtg gttcaagctc ctgtggcagc tattgctatg acggtttttc taatcaattt
      361 gcgtctcttt ttgttgagct tacacgcatc gacttatttc cgtcatacca gtctttggca
      421 caatatcggt atgtctagta tcttgacaga tgagacctat ggcgttttga tgggcgaatt
      481 agcccataca gataaggtca atccgatgtg gatgcacggg aacaatctca acagctatgt
      541 ggcttggttt gtgggaacag tggtcggaac ggctctaggt gacctgttgc caaatccaga
      601 aatctttggt ttagattttg ccctggttgg gatgtttatc gggatttttg cttcgcaatt
      661 ccagatgatg caaagacgga tttctgtccg caatctgctc attatcttag cagttgttgc
      721 agtgtccttc tttttacttt tgacagtggt gtctcagtca ctagctgttc tgtttgcgac
      781 gctacttggt tgtagcatgg ggatggtttt agatggtcag taagtatctt ttattagcag
      841 ttattttctc tggtcttgtg acctggattc cccgtatgat tcccttcatc ttggtcaagt
      901 ataagggcct gcctacgatt gttgagcgtt ttttgaagtt tttgcctgtt tccattatct
      961 ttgccttgat tctttcaaga gtagtgacag gtaaggttgg gaaccttcct caaatcaaat
     1021 gactggactt cttagcagtc tttccaacag cttgggtagc ttttcgctac cgcaatctag
     1081 tcggtacggt tctctttgga gtagtcttga ttgccgtctt gcgtttggtc ttttaaataa
     1141 gtcataaaga aaacttatca cagagataga tatcatataa tggcgtaatt actttttttc
     1201 tgataagatt ataaggtatt ctattttgga ggaaatgaca tgaaaaaaat cgttaaatac
     1261 tcatctcttg cagcccttgc tcttgttgct gcaggtgtgc ttgcggcttg ctcaggtggt
     1321 gctaagaaag aaggacaagc agctagcaag aaagaaatca tcgttgcaac caatggatca
     1381 ccaaggccat ttatctatga agaaaatggc gaattgactg gttacgagat tgaagtcgtt
     1441 cgcgctatct ttaaagattc tgacaaatat gatgtcaagt ttgaaaagac agaatggtca
     1501 ggtgtctttg ctggtcttga cgctgaccgt tacaatatgg ctgtcaacaa tcttagctac
     1561 actaaagaac gtgcggagaa atacctctat gccgcaccaa ttgcccaaaa tcctaatgtc
     1621 cttgtcgtga agaaagatga ctctagtatc aagtctctcg atgatatcgg tggaaaatcg
     1681 acggaagtcg ttcaagccac tacatcagct aagcagttag aagcatacaa cgctgaacac
     1741 acggacaacc caactatcct taactatact aaggcagatt tccaacaaat catggtacgt
     1801 ttgagcgatg gacaatttga ctataagatt tttgataaaa tcggtgttga aacagtgatc
     1861 aagaaccaag gtttggacaa cttgaaagtt atcgaacttc caagcgacca acaaccgtac
     1921 gtttacccac ttcttgctca gggtcaagat gagttgaaat cgtttgtaga caaacgcatc
     1981 aaagaacttt ataaagatgg aactcttgaa aaattgtcta aacaattctt cggagacact
     2041 tatctaccgg cagaagctga tattaaataa tttcttgaaa tcatccattc tgagtaagat
     2101 gggtggtttc tcagttttta gggatatagt tttaaactat atatctgact atctaataac
     2161 aatttgtgaa atcaaacaaa ttgtgagata ctagtacggt attattttta aggagaaaga
     2221 atcatgaaaa tcaaaaaatg gcttggtcta gcagcccttg ctacagtcgc aggtttggct
     2281 cttgcagctt gcggaaactc agaaaagaaa gcagacaatg caacaactat caaaatcgca
     2341 actgttaacc gtagcggttc tgaagaaaaa cgttgggaca aaatccaaga attggttaaa
     2401 aaagacggaa ttaccttgga atttacagag ttcacagact actcacaacc aaacaaagca
     2461 actgctgatg gcgaagtaga tttgaacgct ttccaacact ataacttctt gaataactgg
     2521 aacaaagaaa acggaaaaga tcttgtagcg attgcagata cttacatctc tccaatccgc
     2581 ctttactcag gtttgaatgg aagtgccaac aagtacacta aagtagaaga catcccagca
     2641 aacggagaaa tcgctgtacc aaatgacgct acaaacgaaa gccgtgcgct ttacttgctt
     2701 caatcagctg gcttgattaa attggatgtt tctggaactg ctcttgcaac agttgccaac
     2761 atcaaagaaa atccaaagaa cttgaaaatc actgaattgg acgctagcca aacagctcgt
     2821 tcattgtcat cagttgacgc tgccgttgta aacaatacct tcgttacaga agcaaaattg
     2881 gactacaaga aagcactttt caaagaacaa gctgatgaaa actcaaaaca atggtacaac
     2941 atcattgttg cgaaaaaaga ttgggaaaca tcacctaagg ctgatgctat caagaaagta
     3001 atcgcagctt accacacaga tgacgtgaaa aaagttatcg aagaatcatc agatggtttg
     3061 gatcaaccag tttggtaata agaaacaggg aggtgggaga gaaaattcca cctcttgctt
     3121 ttgtatagag tatagattgt aaagaagact attcgttcat agaaaggtag agagaatatg
     3181 gtttttccta gcgaacaaga acagattgaa aaatttgaaa aggatcatgt agtccaacat
     3241 tattttgagg ttttgcgtac cttgatttct aagaaatcag tctttgccca gcaggttgga
     3301 ctcaaggaag tcgcaaatta tctgggtgag attttcaagc gtgttggagc tgaagtggag
     3361 attgatgaga gctatacagc gccctttgtc atggcacatt tcaagagttc gcgtccagat
     3421 gccaagacct tgattttcta taaccactat gacactgtgc cagcggatgg ggatcaggtc
     3481 tggacagagg atccttttac gctttcggtc cgcaatggct tcatgtatgg gcgtggggtt
     3541 gatgacgaca agggtcatat cacagctcgc ttgagtgctt tgagaaaata tatgcagcac
     3601 catgatgatt tacctgtcaa tatcagcttt atcatggagg gagcggagga atcggcttca
     3661 acagacctag ataagtattt ggaaaagcat gcagacaaac tccgtggggc ggatttgttg
     3721 gtctgggaac aagggaccaa aaatgccttg gagcagctgg aaatttctgg tggcaataag
     3781 gggattgtga cctttgatgc caaggtaaaa agcgctgatg tggatatcca ctcgagttat
     3841 ggtggtgttg tggaatcagc tccttggtat ctcctccaag ccttacagtc tcttcgtgct
     3901 gcggatggcc gtatcttggt tgaaggcttg tacgaagaag tacaagagcc caatgaacga
     3961 gaaatggcct tgctagaaac ttatggtcaa cgaaacccag aggaagttag tcggatttat
     4021 ggattggagt tgcctctctt acaggaggag cggatggcct ttctaaaacg tttctttttc
     4081 gagccagcgc ttaatatcga aggaatccag tctggttatc aaggtcaggg tgttaagact
     4141 attttacctg cagaagccag tgccaagcta gaggttcgtc tggttccggg cctagaaccg
     4201 catgatgttc tggaaaaaat tcggaaacag ctagacaaaa atggctttga taaggtagaa
     4261 ttatactata ccttgggaga gatgagctat cgaagcgata tgagcgcacc agccattctc
     4321 aatgtgatcg agttggccaa gaaattctat ccacagggcg tttcagtctt gccgacgaca
     4381 gcggggacag gacctatgca tacggtcttt gatgccctag aggtaccaat ggttgcattc
     4441 ggtctaggaa atgccaatag ccgagaccac ggtggagatg aaaatgtgcg aatcgctgat
     4501 tatcacaccc atatcgaatt agtagaggag ctgattagaa gctatgagta gagatattat
     4561 caagttagat cagatcgatg tgacttttca ccaaaagaag agaaccatta cagcggttaa
     4621 ggatgtgacc attcacatcc aagaagggga tatctacgga atcgttggat attctggagc
     4681 aggaaaatca acccttgtac gggtgattaa tctcttgcaa aaaccatctg cagggaaaat
     4741 taccattgac gacgatgtga tttttgacgg caaggtgacc ttgacggcag agcagttgcg
     4801 tcgtaaacgt caagatatcg gaatgatttt ccagcatttt aacctgatga gccaaaagac
     4861 agcagaggag aatgtagcct ttgcccttaa acactctgaa ctcagcaagg aagaaaagaa
     4921 ggctaaagta gctaagttgt tggacttggt tggtttggca gatcgtgctg aaaactaccc
     4981 ttcacaacta tctggagggc aaaaacagcg tgtggcaatt gcgcgtgcct tggccaatga
     5041 tccaaaaatc ttgatttcag acgagtcaac ttctgccctt gatccgaaga caaccaagca
     5101 gattttggcc ttgttgcaag atttgaacca aaaattaggc ttgactgttg tcttgattac
     5161 gcatgaaatg cagattgtca aagacattgc caaccgtgtg gcggttatgc aggatgggca
     5221 tttgattgaa gagggtagtg tgcttgaaat cttctcagac cctaaacaac ctttgactca
     5281 agactttatc tcaacagcta caggtattga cgaagccatg gtcaaaatcg agaagcaaga
     5341 aatcgtggaa cacttgtctg aaaacagtct cttggtgcaa ctcaagtacg ctggatcttc
     5401 aacagacgag ccacttttga atgaattgta caagcattat caagtaatgg ctaatattct
     5461 ctatgggaat atcgaaatcc tcgatggtac tcctgttgga gaattggtgg tggtcttgtc
     5521 aggtgaaaaa gcagcgttgg caggtgccca agaagccatt cgtcaagcag gcgtacagtt
     5581 aaaagtattg aagggaggac agtaagatgg aatcattgat tcaaacctat ttgccaaatg
     5641 tctataagat gggttggtct ggtcaggcag gctggggaac agctatctac ctaaccctct
     5701 atatgacagt tctttccttc attatcggag gcttcttggg gctagtggca ggtctctttc
     5761 tcgtcttgac agcgccaggt ggtgtcttgg agaataaagt cgtattctgg attttagaca
     5821 aaattacctc aatttttcgt gcgattccct ttatcatcct cttggcaatc ttgtcaccac
     5881 tttctcactt gattgttaaa acaagtatcg ggccaaatgc agcccttgtc ccactttctt
     5941 ttgcagtctt tgccttcttt gcccgtcagg tgcaggttgt cttggctgaa ttggatggcg
     6001 gtgtcattga ggcggctcaa gcgagcggag cgactttctg ggacatcgtg ggtgtttacc
     6061 tatcagaagg tcttccagat ttgatccgtg tgacgactgt gaccttgatt tcccttgttg
     6121 gggaaacagc tatggccggt gcggttggag ctggtggtat cggtaacgta gccatcgctt
     6181 atggatttaa ccgctacaat cacgatgtga ccatcttggc aaccattatt atcattttga
     6241 ttatctttgc aatccaattc ttgggagatt tcttgactaa gaaattgagt cataaataaa
     6301 aagagccgcg tggctctttt taactgatca gattttctgt gcaaattttt tactcaaggc
     6361 ttgtccaatc aaggcaccca ctagggctcc gatgacaata cttgcgataa atagaaggac
     6421 agttccaggg tttggagcga ccatgatacg gtcgatatat tcttgggatt ttcctcttgc
     6481 cagaagagta gccatatagg ctttgggcgc aatccacata agcaagattg gtcctgttgt
     6541 actaaaggcg aaaataatga aagaaaggaa gttctttgtt ttgtccttgt attttcctaa
     6601 atgagctact tcatctgcta ggaggccaca gataattcca ggaaggaagg aaccagcacc
     6661 gtgtttagtt cccaagaaaa agagggcaat gacaaggccg atagtggtaa tggctccaaa
     6721 gcgcggaact tttgcgacta ggatcatata gacgctaccg ccgacaaggg cagtaaaggc
     6781 aggcgcataa aacatgtttc cagtttggtc aaagagattg cccaaaagga caccaatccc
     6841 catgcagagg aagtaaagaa ctgcaaaaag cagtgtagtt aagatagatt tcttcatgaa
     6901 ttgtctcctg ataatttttt cacaattctc attgtaccac ggtttggtgg gattgtaaat
     6961 ggatactaga agtttttgaa aacgcttgaa atatgatata atggtttcat agttattttt
     7021 aggggaatat catgggtaga tttttagact ttgtctttaa tcgtttcttt ttagggatga
     7081 ttgcgacagc cttcttttgg ctattaactt tagcaggagg gattatcctt ggtctagcgc
     7141 cggctagtgc caccttgatg agcttatatg cagaacatgg ttatagcttt cgggaataca
     7201 gtttgaagga ggcttggtct ctttacaagc aaaattttgt ctcaagcaac ctgattttct
     7261 atagcttttt aggtgtgggt ctagttttga cctatggttt gtatctcttg gtgcaattgc
     7321 ctcatcagac cattgttcat ttgattgcga cccttttgaa tgtcctagta gttgccctga
     7381 tctttttggc ttatacagta tctttaaaat tacaagttta ttttgccttg tcctatcgaa
     7441 atagtctcaa attatccttg attggcatct ttatgagtct agcagctgtg gccaagattc
     7501 tccttgggac tgtgctactt gtagcaattg gttactatat gcctgccctg ctattttttg
     7561 taggaattgg gatgtggcat ttctttatca gtgatatgtt ggaacctgtc tatgaaatca
     7621 tccatgaaaa attggcgaca aaatagaatg aagcagtttt ggctacatac gcttctaaga
     7681 acctatagtt cagtgatgat cattatcatt gcgagttttg caatcttact ctcttacgct
     7741 gactgggatt cacgtgaaaa ggaagcccag agagtagccc agcgtgtaac tgctagaaca
     7801 gtgagtgaaa ttgaatatta ccatagagag tcaacccaga tagctcaggc tttagttgaa
     7861 aaccaagctc gtattgaggg aatctataaa tactttagtc ttagcatgcc agactatttt
     7921 tactggcaat tagagcggaa agcttcgcct tatatatcag tctctctgta tgaaaatgtt
     7981 gatgacctct atgttcgaaa tgattttgta actggagtgg ccattgcttt tcaagattac
     8041 aaggaagtct atgtttctac taaagacaaa cgtagtggag aaaaaatcag ggctgaggat
     8101 ttcaaaccag caggaaatag ttttgccatt ccggtgtcag atccagtgtc agatcaagat
     8161 ttaggagtga tttacatctc cttggatcct gctgttttat accatgccat tgataatact
     8221 agaggtcata ctccgatggc agtaacagtg acctcacctt ttgatacgga gatttttcat
     8281 attggtgaga cagttgataa ggagagtgaa aattggctag ttggcttaac ttctcatgga
     8341 tatcaggttc aggtggcagt tcctaaaaac tttgttttac aaggaacagt gactagctct
     8401 gctttgattg tgggtttgag ccttctcttt attgtcattc tttatctgac tttgaggcag
     8461 acttttgcta attaccaaaa gcaggtagtg gatttagtag aatccattca agtcattgct
     8521 caaggcgaag aggggcgtcg gattgacatt tccgagaaag atcaggaatt actcctaatc
     8581 gcggagacga ccaatgatat gttggatcga ttggaaaaga atatccatga tatttaccag
     8641 ttagagctta gtcaaaaaga tgccaatatg cgagccttgc aggcgcaaat caatcctcat
     8701 tttatgtata atacgctgga gttcttgcgc atgtatgcag ttatgcagag tcaagatgag
     8761 ttggcagata tcatttatga attcagtagt ctcttgcgta acaatatttc cgacgaaaga
     8821 gagaccctcc tcaaacagga attagaattt tgccgtaaat acagctatct ctgcatggtt
     8881 cgctatccca agtccattgc ctatggtttc aagatagatc cagagttaga gaatatgaag
     8941 attcccaagt ttaccttgca accgctggta gaaaactatt tcgcgcatgg tgttgaccac
     9001 aggcggacag ataatgtgat tagcatcaag gctcttaaac aggatggttt tgtggaaatt
     9061 ttggtggtcg ataatggtag aggaatgtcg gctgaaaagt tggcaaatat ccgagaaaaa
     9121 ttaagtcaga gatattttga acaccaagcc agctacagtg atcaaaggca gtctatcggg
     9181 attgtcaatg tacacgagcg ttttgtgctc tattttggag accgctatgc cattacgata
     9241 gagtctgcag agcaagccgg tgttcagtat cgtattacaa ttcaagatga gtagaaaggg
     9301 agaaaatgta taaagtatta ttagtagatg atgagtacat ggtgacagaa ggtctgaagc
     9361 gtttgattcc ctttgataag tgggatatgg aggtcgtcgc aacagccagt catgccgatg
     9421 aagctctaga atatgttcag gaaaatcctg tcgatgtcat catttccgat gtcaatatgc
     9481 cagacaaaac agggcttgat atgattcggg agatgaaaga gatcttacca gatgctgcct
     9541 atatcctgct ctcaggttat caggagtttg attatgtaaa aagagcaatg aaccttagtg
     9601 tggtggacta tttggtcaag cctgttgata aggtagagct gggaaatctg ctggagaaga
     9661 ttgcaggtca gctcggcgag agagggaaga aaagtcagac tcttagtcaa gaattagacg
     9721 aggctggatt tgttagttat ttaggggata aggagaattg gtggataggt ctatccaagg
     9781 aaaaacaagg ttccttcgcc attccctact atgtcttggg ccaagactgg cagattttca
     9841 tttctgacca acccctagat ggtttagtcg ttacaccttt tgaagctcct tatcaagaac
     9901 actttgaacg ctggaagctg aatgctgaga aaaccctctt ttacggttct gtaaatctgc
     9961 agcagtctga gagtctcttt gcctattacg aaccgattta tagggttatc attcagggaa
    10021 atctcaatca aatcgtagaa gagttaaatc tcttggagaa ggtagttctt gaaaatacgc
    10081 cgcgtgttcc gattactaaa cagcttttta ttcagtttgt catggatgtt ttccatttat
    10141 ttgaacatct caaagctgat gacctgacag acatcgtcaa aaccattcat gctattcaat
    10201 ccttcgatga attggttcct tatatcaagg aaactctgac cagctttttc ggtcaatacc
    10261 gtatgaatga aaatgtggtc agtgtgctgg aagtcattgg tcgtgattac caaaaagagc
    10321 tttccctcaa ggatatcagt aaggctctct ttatcaatcc tgtctatcta gggcagttga
    10381 ttaagcgtga aaccgattcg acttttgcag agttactaaa caaacaacgt attaaggctg
    10441 cccaacaact tttgctttca actagtgaca gcatcgaaga tatttgttat gctgttggtt
    10501 acagtaacct tggatatttc tataaagttt ttcgaaaatt gtgcggaaaa tcgccaaaag
    10561 cctaccgaaa acaggtagaa actatactat aagatttgta ttcctttaca aaaggtgcta
    10621 taatattatc aggaggttct atcatgaaaa agaaaccaat ttatctatgg gtcttgctaa
    10681 tcttgtctgc ccttatttca gttccgtctc tgtttggaat tgtgagtccc ttgcctagta
    10741 aagaagccct tcgtgctgct cagaaacaag ttgcaggggt caatgctcag caattagaag
    10801 accagcttaa ttatacctat agagtagcag aagcatctca ttctattttt aatgttgctt
    10861 tgattgtgct atctactatt ttagtggtcg tagcgattgt cttccttgtt cgtaaaaatt
    10921 tgcaatatgc aaactatact tacgttggtt atgttttgtt agctattatt ggttcgattt
    10981 atggctatgt tggtttacaa gacgctgtgc agttggttca agatgagagc atgcgtttga
    11041 cagtgagtat tggttcaaaa gctgttagca ttttctatat cgtcatcaat gttctcttcc
    11101 tagcccttgt cttctataag atgtggcgac aacagaaagc cttggcagaa gaagaggaaa
    11161 cagaagagct tacctaagta ttgacaaaaa agaactatca agatacaatc ttggtagttg
    11221 ttttttgatt atagataaaa tcatggaggt acatatgaag acaatttctt tagtttatat
    11281 tagtttgagt ggcaacactg aaagttttgt gacgcgcttg aaagactatc tcttgtccca
    11341 gtacaaaggg attgaggttc aaaagattca tatcaaggat ttagtcaagg aaggcaaaaa
    11401 tttctatgaa atggaccatc cctatgtcgc ttttttgccg acctatctcg aaggtgggaa
    11461 tggcgtggat aacggagatg ttgagatttt gacgacaccg gtgggagatt ttattgccta
    11521 tggtaacaat gctagtaagt gttttggtgt ggttgggtca ggaaatcgta actttaataa
    11581 ccaatactgc ctaacagcca agcaatacag tcaacgcttt ggcttccctg tattggctga
    11641 ctttgaaatg cgaggcatgc tggaagatat caaacatgtc gcagctatta tcgcagattt
    11701 gtatgagttg gaaaaggaga attaatactc aatgaaaatc aaagagcaaa ctaggaagct
    11761 agtcgcaggt tactcaaagc acagctttga ggttgcagat agagctgacg tggtttgaag
    11821 agattctcga atagtataaa gttcttataa gaggtggttg aagttgttca actgtttttt
    11881 gagtataaac agtctttaag aacgtaaaaa agcataagtt caggtcttca aaaccttgtt
    11941 cttatgcttt ttatcataga aatatttgct gaattttctc ttgaaatgaa accttatctt
    12001 catctcgcgc ttgtccctta ccctgctttt ccgattgcga gtgcgtagat aaagaagatg
    12061 gcgaagccaa agaggaaaat caatcccgca atggcaaggt gtttgagcca agaaggaaga
    12121 tttttgtttt cacgcgttac caaggcgtaa ttcaaaagag caaagaaagg tgttgtcagg
    12181 aaagagccaa tcatggcaaa gcggagcatg gttgaaacct gaccagcgaa gaacttgata
    12241 atgacgatac cgatgatagc agtgatggtc atccagatgt tcaaagattt acgattgtcc
    12301 tctttttgac tgattagcag tcggagagat tcctgattaa cgcgagaata gccatcgata
    12361 acagttataa ctgttccaaa gatacagagg aaggcaataa aggtaatcaa gtaacgggac
    12421 cattcgccaa gaacagaggc atacataccc acgaattgag agatgtattt ggctgaagca
    12481 gcttcaaccg cctgccctgt aggatactga atcagtgctc ccagtgccac aaagaagacg
    12541 gctaggatag ctgttccaat ataaccagtg ttaaagtcaa acagagcgtc ttctgtgtta
    12601 aagttgacgg tctttctctt ttcagctgac caaagtgaat tgatagctga aatttcaata
    12661 ggagccggca tccatcctag gagggagacg atgaagggca gagctgccat ttgccaaggt
    12721 gtcttctcga caaaatcaga actgtattct ggatgcttga ccgccgcaat gataactgca
    12781 agaacagtcg caatggttaa ggcagacata atccatttga ccatgccgtc taaaagtttg
    12841 tagcctccaa agagtagcat agcccaaatg attgcaacga gaatgaggga ccactgagta
    12901 atgctaagtc caatcattgg gaaggcactg gcgatgatag ctgagcacag aatggcaaca
    12961 ccagccgtgt tgaccatagc cgaaaagaca ttgaggataa agaaaatcca gagatagagt
    13021 tttccttttt cggcataacc ttcaaccaaa gtctttccag tatcagctgt gtattcagca
    13081 ccaaaacgga aaaatggata tttaaagaca ttggctaaga tgaccaagag aagtagagac
    13141 caaccgtaag aaccgccagc ttgagttgag gatacaatgt gggaacctcc aacagcggca
    13201 gttgccatta ggattccagg tcccattgct tttagcttgc ttgcccaggt tgattgattt
    13261 aaggaaatag cttgcgacat gataaatact cctttttgaa ttttcagaat aatctgtata
    13321 tgtaatctat attctacaaa aaattttgga aaaatgcaag agtattttga aaaaagatag
    13381 aaaatttaca aagaagatct gaaataactg ttatgatata ga
//
LOCUS       DAVMMR010000053        12493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMMR010000053 DAVMMR010000000
VERSION     DAVMMR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.053"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VYS74_001601"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VYS74_001601"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3789610.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVEYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VYS74_001602"
     CDS             complement(1169..1693)
                     /locus_tag="VYS74_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV3789611.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRK
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VYS74_001603"
     CDS             complement(1746..1982)
                     /locus_tag="VYS74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV3789612.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VYS74_001604"
     CDS             complement(2050..2970)
                     /locus_tag="VYS74_001604"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV3789613.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEIYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3565
                     /locus_tag="VYS74_001605"
                     /pseudo
     CDS             3160..3565
                     /locus_tag="VYS74_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3781..4362
                     /locus_tag="VYS74_001606"
     CDS             3781..4362
                     /locus_tag="VYS74_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3789614.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4359..5636
                     /locus_tag="VYS74_001607"
     CDS             4359..5636
                     /locus_tag="VYS74_001607"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV3789615.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5654..6316
                     /gene="deoC"
                     /locus_tag="VYS74_001608"
     CDS             5654..6316
                     /gene="deoC"
                     /locus_tag="VYS74_001608"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV3789616.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGSDFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6303..6692
                     /locus_tag="VYS74_001609"
     CDS             6303..6692
                     /locus_tag="VYS74_001609"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3789617.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VYS74_001610"
     CDS             6782..7834
                     /locus_tag="VYS74_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV3789618.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVSEK"
     gene            7978..9513
                     /locus_tag="VYS74_001611"
     CDS             7978..9513
                     /locus_tag="VYS74_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789619.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VYS74_001612"
     CDS             9506..10564
                     /locus_tag="VYS74_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789620.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VYS74_001613"
     CDS             10567..11523
                     /locus_tag="VYS74_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789621.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            11608..12493
                     /locus_tag="VYS74_001614"
                     /pseudo
     CDS             11608..12493
                     /locus_tag="VYS74_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3639 a         2207 c         2838 g         3809 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcaggt tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag aatacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctttctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt taatggcgaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc tacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa tttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtt aaaggaggtg ttgtttttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatataa
     3061 tataagtgat caatttattg atgaaatagg aggtagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg atgtgtacta tgaaaatctt aaaatgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tatggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgctc ttaattgctt tcttactaaa aaaaacgatg
     3361 ggcgaaatgg attgctgctg gtagttattg ctttttagtc ctatggattg cgacagaggg
     3421 ttatttcttc cacatgtcac tagaagattt gatacgactt tggacaagtt tggaaatcct
     3481 gacacaaacg tatcagttgg gcttttatct aaatattttg ttaggaattt tgttgatgat
     3541 taagcatttg aaggttaagc aatagtaatc aaaatgcgcc tgatgataga caataaactt
     3601 tttgttttta tctggactgt gatatttata gttctaaatg gtttattatt tataagtgtt
     3661 gaaagagatt gaagaaaatg gcttcgtgac tcgctttctt cggtcttttt cgttttatta
     3721 tcaatttgta aacgatttac aatttgagtc aaattatggt aaaatagtgt catgagtaaa
     3781 atgtattatg cagaaaatcc tgacgctgct cacgacattc atgagttgag agtagagttg
     3841 ttgggacaaa aaatgacctt tttgacggat gcgggtgttt ttagcaagaa aatagttgac
     3901 tttggaagtc aactcttgct caagtgtctg gatgtcaacc aaggagagac agtcctagat
     3961 gtaggctgtg gttatggtcc attaggtttg tccttggtca aggtttacgg agttcaggcg
     4021 accatggttg atattaacac tcgtgccctg gatttagctc ggagaaatgc tgaaaaaaat
     4081 aatgcaaaag cgacgatatt tcaatctaat atttatgcaa gagttcaagg gcgatttgac
     4141 catgtcattt ccaatcctcc tatccgtgcg ggcaagcaag tcgttcatga aatcatcgag
     4201 aaaagtaaag atttcttgga aactggtggg gatttgacaa tcgtcatcca gaaaaaacaa
     4261 ggggctccaa gtgctaagtc caagatggag gatgtgtttg gtaattgtga aatccttaaa
     4321 aaagacaaag gatactatat ccttagaagt gtgaaagaat gagagcagtt gatttaatcc
     4381 aaaaaaaacg agacggtcaa gaactgactt caagtgaaat tgaatggctg atagaaggct
     4441 atgtgtcagg aactgttcct gattatcaga tgtctgcctt tgctatggct gtttatttta
     4501 aaggaatgac gactcgtgaa atttctgatt taacgatgaa tatggttaag actgggcaag
     4561 agtttgacct gtcagccatt gatggtgtga aagttgacaa gcattctact ggtggtgtcg
     4621 gcgataaggt aaccttgatt ttagctcctc ttgttgctag cttcggtgtg cctgtagcaa
     4681 aaatgagtgg tcgtggtctt ggccatacag gcggaacaat tgataaattg gagtccatta
     4741 agggctatca agtagaacgt agtcaagagg attttattcg tcaggttcag gatattggag
     4801 tatctgtcat tgggcaatca gaccagctgg tcaaagcaga taagcttctc tatgcccttc
     4861 gtgatgtgac cgcaactgtc gacacgattc ctttgattgc gagttcggtg atgagcaaga
     4921 aaattgcggc aggggcggat gctattttgc tagacgtgac tgtcggtgag ggtgccttca
     4981 tgaagacggt tgatgaggcg cgtgagttgg ctcaaactat ggtagatctt ggtaaggttg
     5041 ttggacggaa gacagtagca gtgattacgg atatgagcca gcctttggga cgagcgattg
     5101 gaaatcgtct ggaaatcctt gaagcattgg agattttgca aggtcaagga cgtcaggata
     5161 ttacccactt tatttgtgaa ttggctcaaa ttatgcttgg tttggcaaat gttaacaaaa
     5221 ctgttgaaga agttcgccaa catcttgaga atggtcaagc actggctaag tttgaggaaa
     5281 tggtgcaagc ccaaggtgga gacttggaag acctctattg tcctgtaaat gtagctcatg
     5341 tggtggaaat ccctgctcag gaaacgggtg tcatttcagc tcttccagct atggactttg
     5401 gtctttatgc catgaggctg ggtgctggtc gtgcagttaa gtctgatgcc ttggactatg
     5461 aaacaggaat tgtttttgaa aagaagattg gagactccgt tcaaaagggt gaaattgttg
     5521 caaaagtata cacaaatgaa aaaattccac ctcaactagt tacagatttc caaaaatgtg
     5581 ttaaaataag tgatgaagtg aaaaaaatcc gtgaaattgt agaaatcatc tcctaaatgg
     5641 agggaaaacc gaaatgaaat taaataaata tatagatcat acgcttttaa aacaagatgc
     5701 aaagaaaaaa caaattgata gtttgttgtc tgaggctaga gagtatgact ttgccagtgt
     5761 ttgcgttaat ccgacctggg ttgaacatgc taaaaaagga cttgaaggca cagatgttaa
     5821 ggtttgcaca gtagtaggtt tccccttggg agcaacaact tcaactgtga aagcttttga
     5881 aacaaaagaa gctatccaaa atggggctga tgagattgac atggtaatca atgttggggc
     5941 tctcaagtca ggtgatttgg ccttggttga gtcggatatt cgtgcagtag tggaagcaag
     6001 tggggacaag ttagtgaaag tcattattga agcttgcctt ctgacagacc aagaaaaagt
     6061 tttggcttgc caattagccc aaaaagcagg atctgacttt gtcaaaacat ctactggctt
     6121 ttcaactggt ggtgctacga tagcagatgt tagattaatg cgtgaaacag ttggatctga
     6181 tatgggagtc aaggctgctg gtggagctcg ttcttatgca gatgctcttg cctttgtcga
     6241 agcaggtgcg acccgtatcg gaacgtcagc tggggtagct attttaaaag gagaattggc
     6301 agatggcgac tactgagttg attgaactgg caattgaaac cagcaaacat gcctatgtcc
     6361 cctattctca ctttcctatc ggagcagttt tagtagccaa agacggaagt gtttacacgg
     6421 gagtgaatat cgaaaatgct agctatcctt tgaccaattg cggtgagaga acagctattt
     6481 ttaaggctat atctgaaggc caaagagaat tttcagaatt gattgtctat ggtcaaactg
     6541 aaaaaccgat ctcgccatgt ggtgcttgtc gccaagtgat ggctgaattt tttgaacaag
     6601 atctaaaagt gaccttagtc gcaaaagata aatcgacggt cgagatgacg gtcggggaat
     6661 tacttccata ctcttttaca gacttaaact agtctgagtc gctctctgag tggcacggtc
     6721 cttgtggcca accaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcag gcttggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttc ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaga atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 agcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtcc aagttgacta
     7381 cgctggttca tttggtgatg cggctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgatcg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag tttctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aactagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagca
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta tcggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctaccaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg agattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaagaac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta tttctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gcgctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttggct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agcttgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctctataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccctggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgca ccccaaaagt tagacagaaa
    11581 aaatctaact tttggggtgt ttttattatg aaattaagtt atgatgataa agttcagatc
    11641 tatgaactta gaaaacaagg atatagctta gagaagcttt caaataaatt tgggataaac
    11701 aattctaatc ttaggtatat gattaaattg attgatcgtt acggaataga gttcgtcaaa
    11761 aaaggaaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattaa taaagtctga
    11821 catgaaggct ggactaaaga tagagtttct cttgaatacg gtctcccaag tcgtacgata
    11881 cttcttaact ggctagcaca atacagaaaa aacgggtata ctattgttga gaaaccaaga
    11941 gggagagtac ctgagagcgg agaatgccat cctaaaaaag ttaagagaat tccgattgaa
    12001 ggaggaaaaa gagaaagaag aaagacagaa attgttcaag aattaatgac tgagttttcg
    12061 ttagatattc ttctaaaagc cattaaacta gctcgttcga cctactacta tcacttgaaa
    12121 cagctagata aaccagataa ggaccaagag cttaaagctg aaattcaatc catctttatc
    12181 gaacacaagg gaaattatgg ttatcgtcgg atttatttag aactaagaaa tcgtggttat
    12241 ctggtaaatc ataaaagagt tcaaggcttg atgaaagtac tcaatttaca agctaaaatg
    12301 cgacagaaac gaaaatattc ttctcgtaaa ggagacgttg gcaaaaaggc agagaatctc
    12361 attcaaggcc aatttgaagg ctctaaaaca atggaacagt gctacacaga tgtgactgaa
    12421 tttgccattc cagtaagtac tcaaaagctt tacttatcac cagttttaga tggctttaat
    12481 agcgaaatta tcg
//
LOCUS       DAVMMR010000054        12073 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMMR010000054 DAVMMR010000000
VERSION     DAVMMR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.054"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(43..972)
                     /locus_tag="VYS74_001615"
     CDS             complement(43..972)
                     /locus_tag="VYS74_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3789622.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            1112..1399
                     /locus_tag="VYS74_001616"
     CDS             1112..1399
                     /locus_tag="VYS74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV3789623.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(1433..2767)
                     /gene="ciaH"
                     /locus_tag="VYS74_001617"
     CDS             complement(1433..2767)
                     /gene="ciaH"
                     /locus_tag="VYS74_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV3789624.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(2757..3431)
                     /gene="ciaR"
                     /locus_tag="VYS74_001618"
     CDS             complement(2757..3431)
                     /gene="ciaR"
                     /locus_tag="VYS74_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV3789625.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(3540..6086)
                     /locus_tag="VYS74_001619"
     CDS             complement(3540..6086)
                     /locus_tag="VYS74_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV3789626.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(6209..6649)
                     /locus_tag="VYS74_001620"
     CDS             complement(6209..6649)
                     /locus_tag="VYS74_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV3789627.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(6624..7493)
                     /locus_tag="VYS74_001621"
     CDS             complement(6624..7493)
                     /locus_tag="VYS74_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV3789628.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(7535..8263)
                     /locus_tag="VYS74_001622"
     CDS             complement(7535..8263)
                     /locus_tag="VYS74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789629.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(8271..8750)
                     /locus_tag="VYS74_001623"
     CDS             complement(8271..8750)
                     /locus_tag="VYS74_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3789630.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(8761..9459)
                     /locus_tag="VYS74_001624"
     CDS             complement(8761..9459)
                     /locus_tag="VYS74_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV3789631.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(9452..9718)
                     /locus_tag="VYS74_001625"
     CDS             complement(9452..9718)
                     /locus_tag="VYS74_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV3789632.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(9784..10710)
                     /locus_tag="VYS74_001626"
     CDS             complement(9784..10710)
                     /locus_tag="VYS74_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV3789633.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(10785..11600)
                     /locus_tag="VYS74_001627"
     CDS             complement(10785..11600)
                     /locus_tag="VYS74_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789634.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(11604..11924)
                     /locus_tag="VYS74_001628"
     CDS             complement(11604..11924)
                     /locus_tag="VYS74_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3789635.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         3661 a         2703 c         2061 g         3648 t
ORIGIN      
        1 cagaaatgtt tgcatcttcc ttctctcaac gaatcatgta ttttatctaa ccatgtcagg
       61 atcgtagtca taaaatcctg tatcaaggaa acggttttta gggtgaagtt tacgaacttc
      121 ttcatccagc aagaaggctt gatcatggct aaagttgccc tcttgaatca aattacgcgc
      181 ttggataaag agttttgcaa tctcaacaaa gttctgacca ggagtgtaga gcccttctag
      241 tttccagcga gtaaagccat gctccaccaa ttctgttaat ttgatcatca aatcaaggtc
      301 attgttggca aagatatggg tcccatgatt atcttcaaaa atggaatagt ggctctctgg
      361 atcacttggc tcagccaaga agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt
      421 aaagttatag tagttttgca agagtggacg tttagaatga tggatgacgc tagcaccgta
      481 aaccaaaact tcagcaggaa tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg
      541 aatttcacgc gccaaaacag cctcagatgc gccagccttt tgcccccaga agttaatctg
      601 acgactgcta gttaccatgg ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg
      661 gttaactacg taaaagacgc ctgcatcccc aatcgtaata tagtctgtct tgatttcttc
      721 caagaagtct aagaaaggct tgatacggtc catcatatct tggtgcatga gagcattgac
      781 cgcaacgagc aattccttac cagcatcatg aaccaactta gcgatttcac gtaattggtc
      841 ataactaaag gtcgttggca gacgaaggcc aaaatctttc tcaccgacat agatacggtc
      901 tacgccagct tcgagtagtt gttcaacttg ttcaatactt tcagcagttg ctgtaatgac
      961 aatcttttcc ataagaaaaa ttataccaca tttctccaaa atcagccatg ctttgaaaat
     1021 gaaaagggac tttattcttt ttgtaacctt tctgtaataa ttctctgccg aaaaaatgat
     1081 aaaataggta tgtactgtta aggagagaat catgcccgta agaaaattac aatcctatga
     1141 ggtagactat caagaagagt taaaccagca gcttcctcat taccaagctt atacacctga
     1201 agcgcaatct gatgccaatc tcaaggaaat cctatttttt attaatatcg ctgttttttg
     1261 tatctgtatt gctatcttta gttttatctt tttagcatta aaattatcaa ctgctcttgc
     1321 ctttgccgca gcaatcggat tcagcttact tgttttaaaa gttcaacggt ctattatcaa
     1381 acgaaaacgt agaagataaa tccaaaatat cgccccaatt ggagcgatat ttttattttt
     1441 tctttttaga tggtgtctga atggcaatct tcacttcaaa gattgttccc ttgggtttat
     1501 tatctttgac agtaacagtt ccttttagag catctacaat ttgcttggct agggataatc
     1561 ctaaaccaaa accacctttt tgccgggttc tagctttgtc tactcgataa aaacggtcaa
     1621 aaattttctt tttatcttct gtcgaaatac cgattccatt atcagaaaca agtaaataaa
     1681 gattgcgatc ggtcgccgag ataagaaaat caatttcacc atcctcctca gtatacttga
     1741 cggcattatc gaaaagaatg gtcatcagtt gtttcagaag aagctgatct gtgacaattg
     1801 ttcgatggat acgattttca aaacggaaga cacgattatt ttccgaagca atcatctcgt
     1861 agtttgtgaa agttgtatta aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc
     1921 catcatctct ccgagctaag ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt
     1981 cttccaaact cgatgcaatg ctttcgctca catccataat ggtagcttct ggcttacgaa
     2041 aaagggtctc taagcgattt tgcaaaactg cgagtggagt tcgtaactca tgactggcat
     2101 tttccacaaa agactgttgc ttctgcatac tctcaagcag gggcctaaca ctgaccctag
     2161 ctagatagag actggcaagt aaagacaaaa tccagaaact agccatcacg accacaatca
     2221 attgctcatg cttttgacta gcctgttcca actgactggt attaatcaag acagcagcat
     2281 acttgatatt ggttgaaacc gaactaatat tggtctccat caaaatcaca cgatagattt
     2341 cttcctgccc atagctatta aaaacctgaa tctggtagat atgtcctagt tctttctttt
     2401 ctaacttaat cttatccaag cccaaaaatc gatttccaga aagaagttga gtaaagtctt
     2461 tatcaaagag aatgacttcc gtattggaac tgacattagg ttttatttct actttactag
     2521 cgtccgccct agcattttct aaatctttaa tctcttctgt tgccctattt atagccagct
     2581 gaataactgc ttgaggattt tcactcagtc catgaagctt atcgtccacc gaagtataaa
     2641 gactcgaatg catgacttgt aaaataatca gagtcattgt agaaaaaatc agggtgaaga
     2701 caccgaagtt gcggataaaa taactaaagt catccgcata ccatgttttt ttaagtttac
     2761 tgaacatctt ttaaaagata cccaacacta cgcaaggttt gcaaattctc tgcaaaagtg
     2821 gttcccttta atttcttacg gacttttgaa acatagactt cgacaaccga aatcgttgta
     2881 tcactatcaa atccccataa acggtcaaaa atctgcgtct taggcaaaat cacattttga
     2941 ttttgaagga aataaactag taaatcgaac tctttcccca gcaattcgac aggagtatct
     3001 tcaactttaa cggtattggt tgataaatta accacgatat tcccataagt caaggtgttt
     3061 tcattaaact tccctgaacg tttgagaagg gcctgaatcc gcattttaag ttcttctagg
     3121 tagaaaggtt tggtcagata atcatccgct cccagttcaa atccatgtcc cttgtcatcc
     3181 aaactttcct tggcagtcat aatcagaact ggtgtcgtaa ttcccttttc acgcaattct
     3241 tttaagactt ggaaaccatt tttttctggc aacatcaaat ccagcaaaat caagtcatag
     3301 acaccactct cagcttcgta gagaccttct tctccatcaa atacctgcat aacatccgca
     3361 aaatcgtcta aaaagtcaaa tactgaattt gacagaccta ggtcatcctc aaccaataag
     3421 atttttatca tgagaaactc ctccttatta aaactattat accaaatttg ccttaaaaaa
     3481 aactcaactc tctgcatttt acatgagata gctgagtttt ctttttattt taggcttatt
     3541 tatgcatttc cgtattgaag aacaactgct tcgactgcag ctttttcacg gctaatcaag
     3601 tcaacacgtg ctgcaatttc cttgattccc ataccgatgt tacggctaag agcaaggtca
     3661 gaaagttgcg gctcaaagaa ctccttgtat tccgccaagc gttgctgagt cttaaataca
     3721 tgagcaggaa ggataacaaa gctatcaaag ctcatatctc ctccaagggc tgccttaatc
     3781 caagcccagt tttcacgcgc ccaagaccaa gctgttttct gagttgcttg atgagctagg
     3841 aattgataat accaagcaga caagtcctgt ggtttgacca caaatttgtc cttccaagaa
     3901 gtaatcaagt tttggatatt atccgcatct gtactgtatg caagagctgc tgccaactgg
     3961 cgtttaaaga cagcatctgt tgcgtgagta taagtatcaa gataaagtgc taacaagtct
     4021 ttagtctcat gatgtttcat ctcattaatc agaacttgtg aacgaatagc tgctgggagt
     4081 cctgcaagat tctccttgtg tgttgcgaag atttggctag cgacttgact agcttctgca
     4141 tcatttgagc gaatcatcat cgaaacagcc agctgacgaa ccaattcatc ctcatctgat
     4201 tctccgtctt tagcttcaaa accaagacgg tcatagttat gacgagccaa tttagcaacc
     4261 agtcctttga aggctgtttc agcatccgtt ccttcatcaa taaagcgctc aagggctgaa
     4321 atcacttgag aaacagctga aaccaccaga taagactctt ccttagcaag tttatcaaga
     4381 actggaagca agtctgcata agaaatgtgc cctgcctcag ccaacaaacg acgttcttga
     4441 acaatttgca gtttgcttgt gttatcaagt gtctctagct cagcaagaac agcttctaac
     4501 aagtctcctt gatagtcggt aatatagtgg gcagtatttt cagtgttgag acgaagagct
     4561 ccttcatttt cagcaagaag agctgcgtag ccagggattt cgatactttc agtttcgagt
     4621 gtatcaggca agcctttcca gttgctattg aggggcacca cccagagacg gttcttgtct
     4681 tcgttctcac cgatgaagaa ttgtttttgt gaaatcttca agacatcatt ttcaactttg
     4741 acagtaagaa ctgggtaacc aggctgttcc aaccaagaat ccatgaaggc tgcgacatca
     4801 cgtcctgacg cttgaccaag ggcatcccaa aggtcactac caatggtgtt gctgtattgg
     4861 tgtttttcaa agtaggcgtg caaaccttta gcaaaatcag catctcctag ccaacggcga
     4921 agcatgtgca tgagacggct tcctttggca tagacgatag cgccgtcaaa gagtgtattg
     4981 atttcatctg gatgtttaac ttcgacgtgg acagactgaa cgccatcagt agcgtcacgt
     5041 tcaagagcaa gaggtactcc acctgtttgg aaatcttcaa agatattcca gcttggttcg
     5101 atggtatcca cacagacgta ttccatcata ttagcgaaac tttcattgag ccaaaggtca
     5161 tcccaccatt tcatagtcac gaggttccca aaccattggt gagccaattc atgggccaca
     5221 acaagggcaa cttgttgacg gctagcaaat gtagagttct catcgacaac caagtaaact
     5281 tcacggtaag tcacaagacc ccagttttcc atagcaccag ctgagaagtc aggaagggcg
     5341 atgtggagag attgaggaat tgggtactta actccatagt aatcttcgta aaactcgata
     5401 gagcgaacag cgatatccag tgagaaatca agatttgaaa gtggatgtgc tttggttgag
     5461 tagacaccta ccagggtacc atttttagtt ttagcggtca ccccttgcaa atcaccagca
     5521 acaaaggcca acaagtaaga agacatgcga ggtgttgtct caaacttcca gatacctgtt
     5581 tccttacggt tttcaacatc gatttctggc atgtttgaca aggccaattc accttctgct
     5641 tggtcaaagc gaagagagag gtcaaaagtt gctttggctt caggctcatc cacacatggg
     5701 aaagcttcgc gcgcaaaatg gctctcgaac tgagtagaca agacctcctt cttgactcca
     5761 tcaactgtat aataagaagg gtaaatccct gtcatgttgt ctgtaatttt accagaaaag
     5821 gcaagaacca attcaacttg accagcctca gccaattcga tatgaagggc ttcattgtca
     5881 tggtcaactg taaatggacg agcttgacct gcaacttcta cagaggtgat ttccaagtct
     5941 ttttggtgga gggagatgcg gtcactctgt gcttgaccag tgatggtcac tttcccagaa
     6001 aaagtcttgg tctcacgact caaatctaaa aataaatcat aatgttcagg aacaaattgc
     6061 ttaataaaat gttcaactgc ttgcatagtt ttctcctatt ctaagtttaa gagctgctgc
     6121 tcttctacaa acaaacttat tatatcatat tttgcttgaa aaaaaaggat tggacagcga
     6181 tttccaaaac tttcttcctt ctaatagtct acagtgggta gaccttgcga aattcttcta
     6241 aaacaacctt gctttcagat gtaaaagtca gccctacttc acccaaacag tctgtgaaaa
     6301 agtcctcatg aacctggcgc caataggcaa gtgatttgtc tccctcacct tccttaaagg
     6361 catgttgcgc agaaacttga ttgaagagtt caacagaaac ctttgtaatt tcgacaatgc
     6421 agacagactg attttgacta tctaaaatga tatcgaaggt ccctacttgg ggaagtggtt
     6481 cgtcttctag tacatagagg tcataggctg atgctgttgc tgtcttttct cctttaaaca
     6541 ccaaatccgc taaaagatct ggttcaactc caaaagccca agcatcgatt tcatctccga
     6601 tcaaaggatt gatttgcttg tatttattcc acatttcttg cggtatcatg ggtactcctt
     6661 tgtaattttt tactttcttc ttttatgtgt ttaagatgat ctggatggtc aatctctaaa
     6721 tcaaaaatct ctggaataga actgtagtgg ataatgcact tgatacccaa ctgattcatt
     6781 ttttgtatga aagaagtatt cagatagcct gctacagcaa aatcaatctt gttctttctt
     6841 gctttatcct gcatatctct tagcatatct aacattattg gactttccat atcatgccat
     6901 tgactgtttc tcatagtcgc aaaaacaaag gaagtcaaat cattcattcc aactacaatc
     6961 tttgaaatgc ccgtttccag tatactagat aagtcaaaat acgctgacgg taattcaatc
     7021 atcgttccga ctttcccagt aaaaccctgc tgacgcaata ctgtaatagc ttgttttaat
     7081 tggtcgtcat cattgacaaa aggaaagata acagatagat tggggttggt ttgataaact
     7141 tctgtaacga catgtgcttc agcctgaaat tcatccaaac acgccagtaa acgcctggtt
     7201 cctctatagc caaacaaggg atgcccttcg tcaaaaaact ctctagtccc cactaaacaa
     7261 ttggcttctg tattcgttaa ttcagaaaaa cgataccaaa cttcttcatc tgagtacagg
     7321 gagcagatag tctctagata atcttttaca aattgctgac aacttgctaa tatgatgttt
     7381 tgattaagct ctctcaacaa atattcccca cgaatcatgc cgacgtggtg aaatagttga
     7441 ggataaattt tttcaagaat tttttcgcca ctaagggcaa gttgatttct catagccatt
     7501 cacctcgtct taaaagatac aatatcatat ataattatat caaaatttcc tctagttcaa
     7561 aagcttcata aggttcagat aactcatatc ctaaaaattc tgctactttt ttggaagcct
     7621 cattatgagc atcccaaagt ggaaacatat ctctatttaa actctctaga atcattgcag
     7681 caccaagctt tttagcaaat ccatttcctt gttcgtttgg tctagttgca acttccactt
     7741 caactgcttt acggtaaact aaccctgagg aaatcccagc aatcagttca ttatttttaa
     7801 gaatcacaaa gccaaatcca ccttttaaaa caaaatcctg ataggactca aaatccccct
     7861 gtaaatcctg tgaccattct tccgtagaaa agcagttata aatatgatta tcaataggca
     7921 caatattgta accttcctct aaatgagtaa ctagattatt taaaaattca acttgaaaat
     7981 ttgccttatc tttaaaagaa tagcgagtaa aagaatttag ccccactttt cgatgcagaa
     8041 aatcttgcca attagtattt tctgaaataa taatcttata atctaaacca tattttctaa
     8101 caaaatcttc ccaaaatctg gaatccaact cccctgctag gtatagaaaa ttcccaacat
     8161 cataaaagct tgttgtccta tcattgtttc ggtagacagt tccaatttga gatttaagac
     8221 catagagaac catatttttc ttccatgttt taaatagttc cattatccct ctacttcacc
     8281 ttccaattca tgtaagaagt cttgtccagt tctggcaatc ctaataattc agacttaacc
     8341 ttcaagacta atggcgatgc attttcttct gtaatctctt gaatatccat ccaaatatat
     8401 ccaagtgaat cattcgcacc atcagacaca gcttccgaaa tcgtaacttg aggtgcactc
     8461 tcattcattt caacatcata caaggctatg acatggtgaa ccataaaatt ttttaactct
     8521 tccctgacga aaacatcgta gattcgagga ttagagtagc ttctaacagt aaatcccgtc
     8581 tcttccataa cttctctagt cagcgtttcc gtcagtcctt caccaagttg ctgactgcct
     8641 ccaggtagat cataccgatg ttgataaggg cctctcgttt tttcaatgca aagtaacttt
     8701 ccattttcaa agcaaacaca gtagacccca aagtgatttt tgatttccat ccaactcctc
     8761 ctacttcaaa gaccagccac catctattgt cagaatttgt ccctgcatgg cagatgcctt
     8821 tccactggcc aaaaagagac tgacttctgc cacttcctct ggttcaatcc agcgcttgat
     8881 tggtgtttca ctagccaccc agtcagctaa gccacctgtc tcaaaatccg cagcggtcat
     8941 agctgtcttg actgctcctg gagcgatacc aaagacctga atcccagctt cagcatagtc
     9001 tagagccaac tgcttggtga agccagccaa ggcatgcttg gatgaagtat aggcgtgacc
     9061 acctccacct gctaggctag aagcaatgga acacatattg atgatgattc cctttttatt
     9121 ttccagcatt tgtgtcaaat aataccgagt caactctact ggaataatgt agttgatttc
     9181 aaaaatctct tgaatgtcct gcgccgtttg ttccaacagt ggtttgtaag catccaaaac
     9241 tccggcagta ttacacaaaa catccacctg agggcaccag tcaaaaatag gttccaagtc
     9301 caaggtcaaa tctctctgta aaaagtgaaa atcaccctct aagagtggct tttcaccttg
     9361 gtcaactcca taaacttgat agcccttctc taaaaagagg cgagcttgag ccaatccgat
     9421 ccctgaactc actcctgtaa tgagtacacg tttagtcatg cacttctacc caatccgttg
     9481 ccaaaacatc acaaactgtc gggctccaca tggaaaaacc ttctccttct ccagaaacgt
     9541 tgattaggaa ataaggtgtc atttcaagtg caagcccatt ttgctcgatg gtatcaaaga
     9601 gttggacata gttttccgca cctccccaac cagttcgtac atattttctc ttagccttta
     9661 acccaggcag gatctcttca aatgtcatgt ttttctcctt taattctaca ttcttcattt
     9721 aattatagca aaaaaccact ttatacggct ttttgactgt gagttattca aacctgctac
     9781 tacttacggc aaattattcc ctgcagcaag ataaatttca taccattctt ttcttgttaa
     9841 gctaaagttt gccgctcggc taacttctct caagtgctta ggatttgttg tacctacgac
     9901 tgcctgcatt tttgctggat aacgcaatat ccaagaaatg gcaatagttg aagaggttac
     9961 tccatattta atagctaaac gatcaagtac ttgatttaaa gcttgaaatt tctcatttcc
    10021 aacaaagttc cctttaaaat acccgaattg caagacagac catgcttgaa tgaccacatc
    10081 gtgtaattgg caatattcaa aaatgctgcc atctcgcata gctgcttgac tatcttccat
    10141 attaacatga aaagctgatt caaatcctgg agtaaaagcc gcactcaatt gtagctgatt
    10201 aacagctaac ggctgcttga catctttttt aagcaactcc atcatcatag gattttgatt
    10261 agaaactcca aaatctcgaa ctttaccttg tttataaagg agatcaaagg cttctgctac
    10321 ttggtcagat tccatcaaag catctggtcg atgaaggagc aagctatcta gatgatcaat
    10381 cttcaatctt tgcaaaatac cgtctactga ttttataata tagtccttag aaaaatcaaa
    10441 ataggtaaat tcttcaatgc gaatgccaca tttggactga atccacatct tttctcttaa
    10501 atctggacga ttttttagga caagacctaa cagttcttca caacgaccac gaccataaat
    10561 atcagccaag tcgaaggcat tgattccaac agaaagtgct gtttctacaa gctcttcaac
    10621 ttcttttaca gacttatctt ttattctcat cattccgaga acaatttctg ataattcttt
    10681 gtcatcttga ccaagagtta tgtatctcat caaatttttc tcctttaatt tctaacattc
    10741 ttcccttcat tataacaaaa aaccgctttg caacggcttt ttgactatac ttcactccat
    10801 tttatcttct taaacccacg gaacaagaca aaaatgccaa taaagaggac agctaaagga
    10861 ataacttttg taaggaaaac atttgaaatt cccatccact cataataacg gagcagagaa
    10921 cccaccacaa gatgggcaat aatcatactg acaaatggac gaaagaccgc ttctttccaa
    10981 ttccaaatac cgataactag cgaaatcgta aagacagaca aactatccca gggagccgga
    11041 atataaaagg ctccttcttg tatgaagctt gccattccta catatcctaa aacaactaga
    11101 agaactatag tcccaacaac aatgtaagtg ccaattttca ttttaggaga atcttggact
    11161 aaacttcttc gtaaaattgt ggccacaagt ccaaatccaa tcagaaaaat aagaagttgc
    11221 cctaaaaatg tgagcaaatt gactgttaag agaggacctt tagaaaaatc acttagtagt
    11281 tgataataac gtaataccgc caggacaaga attggcgtca aaagggactc tttgatagaa
    11341 ctgcgaggtg ctcccttgag aatctctttc attatttttt taggattctt acctagataa
    11401 tcctctgcac tcatgccatc tcgttctgct tctgagaaat ctagcatcat caaatagatc
    11461 tgctctctga gatagtcttc atcatagaga aatccagcaa gattaaaact ttcccacaac
    11521 tcctcaaaat acttttgatt ctcctcagaa aactcatgta gcaaagcgct tgtttcttcg
    11581 taatacttca ttttcttcat ggtttaaccc ccattcttaa tcccttctac tttttgactc
    11641 aaatcgtccc attgttgcca aaagactgag acacgctctt ctccttcttt cattaatgaa
    11701 aaatacttcc gatctggacc atctggcgac gggcgcatgt cgcctcttat ccattgattt
    11761 ttttctaact tttgcaacaa aggataaata gttcctggaa cgatagtatc aaatccagcc
    11821 tctcgcaaag tctgaaccaa ctcataacca taccgctctt tttgaccaat catatccaag
    11881 acacaacctt caagaacacc ttttaatagc tgagtttctt tcatcacttt tcccttctaa
    11941 tctattttgt aatacctact agtaacttca cctatagtat atcacttcta cactagtttg
    12001 taaagcataa tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccctac
    12061 cttatgtatg gtt
//
LOCUS       DAVMMR010000055        12014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMMR010000055 DAVMMR010000000
VERSION     DAVMMR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.055"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(128..1759)
                     /locus_tag="VYS74_001629"
     CDS             complement(128..1759)
                     /locus_tag="VYS74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV3789636.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2052..7031
                     /locus_tag="VYS74_001630"
     CDS             2052..7031
                     /locus_tag="VYS74_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV3789637.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKYLVALLKEDKSAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTASHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7121..8317
                     /locus_tag="VYS74_001631"
     CDS             7121..8317
                     /locus_tag="VYS74_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV3789638.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8453..8980
                     /locus_tag="VYS74_001632"
     CDS             8453..8980
                     /locus_tag="VYS74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3789639.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9057..9413
                     /gene="glnR"
                     /locus_tag="VYS74_001633"
     CDS             9057..9413
                     /gene="glnR"
                     /locus_tag="VYS74_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV3789640.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9450..10796
                     /gene="glnA"
                     /locus_tag="VYS74_001634"
     CDS             9450..10796
                     /gene="glnA"
                     /locus_tag="VYS74_001634"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV3789641.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10892..11011)
                     /locus_tag="VYS74_001635"
     CDS             complement(10892..11011)
                     /locus_tag="VYS74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV3789642.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11499..12014
                     /locus_tag="VYS74_001636"
                     /pseudo
     CDS             11499..12014
                     /locus_tag="VYS74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3865 a         2198 c         2570 g         3381 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatcta cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag acgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 ctttcttagc atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga cgactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccagt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgatttttcg
     1381 taaaaaagag acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtaataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc
     1681 ccatggtctt gatactatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ctattttaaa ggataaaagt aaatctaaca agtgttaacg tcaagtttgt aaaagaaaat
     1861 tataattgat aagtgtatta tttttaagag tttttaacct tattttagat ttatgaaagc
     1921 agctattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
     1981 gtttgataaa aaaccacaga atataaagcg ctttcttgaa aacaacaaaa tcaatctttt
     2041 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2101 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg
     2161 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2221 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatgct agagaaaaca
     2281 agctagaaaa ggcagaagga gtagcgatag catctgaaac tgcttcgcca gcaagcaatg
     2341 aagctgcaac tactgaaact gcagaagcag ctagcgcagc taaaccagag gaaaaagcaa
     2401 gtgaggtggt tgcagaaaca ccatctgcag aagcaaaacc taagtctgac aaggaaacag
     2461 aagcaaagcc cgaagcaact aaccaagggg atgagtctaa accagcagaa gaagctaata
     2521 agactgaaaa agaagtccag ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa
     2581 aggaaatcaa atttaattct tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag
     2641 atgatgctat taaccgcgga tctgttgtcc tcgcttcacg tcggacaggt catttagtca
     2701 atgaaaaagc tagcaaggaa gcaaaagttc aagccttatc aaacactaat tctaaagcaa
     2761 aagaccatgc ttctgttggt ggagaagagt tcaaggccta tgcttttgac tattggcaat
     2821 atctagattc aatggtcttc tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag
     2881 gtcaccgtaa cggggttcct gtatacggta cactcttctt caactggtct aatagtattg
     2941 cagatcaaga aagatttgct gaagctttga agcaagacgc agatggtagc ttcccaattg
     3001 cccgtaaatt ggtagacatg gccaagtatt atggctatga tggctatttc atcaaccaag
     3061 aaacaactgg agatttggtt aaacctcttg gagaaaagat gcgccagttt atgctctata
     3121 gcaaggaata tgctgctaag gtaaaccatc caatcaagta ttcttggtac gatgccatga
     3181 cctataacta tggacgttac catcaagatg gtttgggaga atacaactac caattcatgc
     3241 aaccagaagg agataaggtt ccggcagata acttctttgc taactttaac tgggataagg
     3301 ctaaaaatga ttacactatt gcaactgcca actggattgg tcgtaatcct tatgatgtat
     3361 ttgcaggttt ggaattgcaa cagggtggtt cctacaagac aaaggttaag tggaatgaca
     3421 ttttagacga aaatgggaaa ttgcgccttt ctcttggttt atttgcccca gataccatta
     3481 caagtttagg aaaaactggt gaagattatc ataaaaatga agatatcttc tttacaggtt
     3541 atcaaggaga ccctactggc caaaaaccag gtgacaaaga ttggtatggt attgctaacc
     3601 tagttgcgga ccgtacgcca gcggtaggta atacttttac tacttctttt aatacaggtc
     3661 atggtaaaaa atggttcgta gatggtaagg tttctaagga ttctgagtgg aattatcgtt
     3721 cagtatcagg tgttcttcca acatggcgct ggtggcagac ttcaacaggg gaaaaacttc
     3781 gtgcagaata tgattttaca gatgcctata atggcggaaa ttcccttaaa ttctctggtg
     3841 atgtagccgg taagacagat caggatgtga gactttattc tactaagtta gaagtaactg
     3901 agaagaccaa acttcgtgtt gcccacaagg gaggaaaagg ttctaaagtt tatatggcat
     3961 tctctacaac tccagactac aaattcgatg atgcagatgc atggaaagag ctaacccttt
     4021 ctgacaactg gacaaatgaa gaatttgatc tcagctcact agcaggtaaa accatctatg
     4081 cagtcaaact atttttcgag catgaaggtg ctgtaaaaga ttatcagttc aacctaggac
     4141 aattaactat ctcggacaat caccaagagc cacaatcgcc gacaagcttt tctgtagtga
     4201 aacaatctct taaaaatgcc caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg
     4261 atgcagattt ctatgaagtt tatgaaaaag atggagacag ctggaaatta ctaactggct
     4321 catcttctac aactatttat ctaccaaaag ttagccgctc agcaagtgct cagggtacaa
     4381 ctcaagaact gaaggttgta gcagtcggta aaaatggagt tcgttcagaa gctgcaacca
     4441 caacctttga ttggggtatg actgtaaaag ataccagcct accaaaacca ctagctgaaa
     4501 atatcgttcc aggtgcaaca gttattgata gtactttccc taagactgaa ggtggagaag
     4561 gtattgaagg tatgttgaac ggtaccatta ctagcttgtc agataaatgg tcttcagctc
     4621 agttgagtgg tagtgtggat attcgtttga ccaagccacg taccgttgtt agatgggtca
     4681 tggatcatgc aggagctggt ggtgagtctg ttaacgatgg cttgatgaac actaaagact
     4741 ttgaccttta ttataaagat gcagatggtg agtggaagct agctaaggaa gtccgtggta
     4801 acaaagcaca cgtgacagat atcactcttg ataaaccaat cactgctcag gactggcgct
     4861 tgaatgttgt cacttctgac aatggaactc catggaaggc tattcgtatc tataactgga
     4921 aaatgtatga aaagcttgat actgagagtg tcaatattcc gatggccaag gctgcagccc
     4981 gttctctagg caataacaag gtacaagttg gctttgcaga tgtaccggct ggagcaacta
     5041 ttaccgttta tgataatcca aattctcaaa ctccgctcgc aaccttgaag agcgaagttg
     5101 gaggagacct agcaagtgca ccattggatt tgacaaatca atctggtctt ctttattatc
     5161 gtacccagtt gccaggcaag gaaattagta atgtcctagc agtttccgtt ccaaaagatg
     5221 acagaagaat caagtcagtc agcctagaaa caggacctaa gaaaacaagc tacgccgaag
     5281 gggaggattt ggaccttaga ggtggtgttc ttcgagttca gtatgaagga ggaactgagg
     5341 acgaactcat tcgcctaact cacgcaggtg tatcagtatc aggttttgat acgcatcata
     5401 agggagaaca gaatcttact ctccaatatt tgggacaacc agtaaatgct aatttgtcag
     5461 tgactgtcac tggccaagat gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc
     5521 aggaaccgaa aaaagattac ctagttggtg atagcttaga cttgtctgaa ggacgctttg
     5581 cagtggctta tagcaatgac accatggaag aacattcctt tactgatgag ggagttgaaa
     5641 tttctggtta cgatgctcaa aagactggtc gtcaaacctt gacgcttcgt taccaaggtc
     5701 atgaagtcaa ctttgatgtt ttggtatctc caaaagcagc attgaacgat gagtacctca
     5761 aacaaaaatt agcagaagtt gaagctgcta agaacaaggt ggtctataac tttgcttcac
     5821 cagaagtaaa agaagctttc ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc
     5881 atgaaactag cacccaagat caagtcaatg accgacttaa taaattgaca gaagctcata
     5941 aagctctgaa tggtcaagag aaatttacgg aagaaaagac agagcttgat cgcttaacag
     6001 gtgaggctca agaactcttg gctgccaaac caaaccatcc ttcaggttct gccctagctc
     6061 cgcttcttga gaaaaacaag gccttggttg aaaaagtaga tttgagtcca gaagagcttg
     6121 caacagcgaa acagagtcta aaatatctgg ttgctttatt gaaagaagac aagtcagcag
     6181 tcttttctga tagtaaaaca ggtgttgaag tacacttctc aaataaagag aagactgtca
     6241 tcaagggttt gaaagtagag cgtgttcaag caagtgctga agagaagaaa tactttgctg
     6301 gagaagatgc tcatgtcttt gaaatagaag gtttggatga aaaaggtcaa gatgttgatc
     6361 tctcttatgc ttctattgtg aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat
     6421 ttttcttacc tgaaggcaaa gaggcagtag aattgacttt tgaacaaacg gatagtcatg
     6481 ttatctttac agcatctcac tttactcatt atgcctttgt ttatgaatct gctgaaaaac
     6541 cacaacctgc taaaccagca ccacaaaaca cagtccttcc aaaacctact tatcaaccgg
     6601 cttctgatca acaaaaggct cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc
     6661 gtcaagagca tgaaaatgct gagatgctag ttggggaaca acgagtcatc atacagggac
     6721 gagatggact gttaagacat gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt
     6781 caacagaagt catccaagaa gcgattccag aaattgttga aattggaaca aaagtaaaaa
     6841 cagtaccagc agtagtagct acacaggaaa aaccagctca aaatacagca gttaaatcag
     6901 aagaagcaag caaacaattg ccaaatacag gaacagctga tgctaatgaa gccctaatag
     6961 caggcttagc cagccttggt cttgctagtt tagccttgac cttgagacgg aaaagagaag
     7021 ataaagatta aatatcgaaa aatcttgtga aatctttcct tatatttcca aagtgtgata
     7081 taatagtttc gaataaaata aataaaggag tttttgtaac atggcaaaac ttactgttaa
     7141 agacgttgac ttgaaaggta aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa
     7201 agatggcgta atcactaacg ataaccgtat cacagcagct cttccaacta ttaagtacat
     7261 catcgaacaa ggtggacgtg caattctttt ctctcacctt ggacgtgtga aagaagaagc
     7321 tgataaagct ggtaaatcac ttgctcctgt agcagctgac ttggcagcaa aacttggtca
     7381 agatgttgtt ttcccaggtg tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct
     7441 tgaagatgga caagttctct tggttgaaaa cactcgttac gaagatgttg acggcaagaa
     7501 agaatctaaa aacgatcctg aacttggtaa atactgggca tcacttggag atggtatctt
     7561 cgtaaacgat gcattcggta cagctcaccg tgcacacgca tctaacgttg gtatctcagc
     7621 aaacgttgaa aaagcagttg ctggtttcct tcttgaaaac gaaattgcct acatccaaga
     7681 agcagttgaa actccagaac gtccattcgt ggctatcctt ggtggttcaa aagtttcaga
     7741 caagatcggt gttatcgaaa acttgcttga aaaagctgat aaagtcctta tcggtggtgg
     7801 gatgacttac acattctaca aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga
     7861 agacaaattg gatgttgcga aagctcttct tgaaaaagca aatggtaaat tgatcttgcc
     7921 agttgactca aaagaagcta acgcatttgc tggttacact gaagtgcgtg acactgaagg
     7981 tgaagcagtt tctgaaggct tccttggtct tgacatcggt ccaaaatcta tcgccaaatt
     8041 tgacgaagct ttgactggtg ccaaaacagt tgtatggaac ggacctatgg gtgtatttga
     8101 aaacccagat ttccaagctg gtacaatcgg tgtgatggac gctatcgtga aacaaccagg
     8161 agttaaatca atcatcggtg gtggtgactc agctgccgca gcgattaacc ttggccgtgc
     8221 agacaagttc tcatggatta gtacgggtgg tggagcatca atggaacttc ttgaaggtaa
     8281 ggttcttcca ggacttgcag ccctgacaga aaaataagat tttataaaca aatcaaagaa
     8341 gagagggatg aaagttcctc ttttcttttg cttaaaataa aaacgcttcc tctcaactat
     8401 tactcataaa aatcaccgat ttatgataaa atggaaatag aaagttgaga ttatgagtta
     8461 ttttaaaaaa tataaattcg ataaatccca gttcaaactt ggtatgcgaa cctttaaaac
     8521 aggtattgct gtttttctag ttctcttgat ttttggcttt tttggctgga aaggtcttca
     8581 aattggtgct ttgacagccg tttttagcct gagggagagt tttgatgaga gtgttcattt
     8641 tgggacttcg cgtattctag gaaatagtat cggtggactc tatgccttgg tctttttctt
     8701 attaaatacc tttttccacg aagccttttg ggtgaccttg gtagttgttc caatctgcac
     8761 catgttaacc attatgacaa atgtagccat gaataacaaa gcaggggtta ttggtggtgt
     8821 agcagctatg ttaatcatta ccctatcaat tccaagtggt gagacaattt tgtacgtgtt
     8881 tgtgcgtgta ttagaaacgt ttatgggagt ttttgtcgca attatcgtaa attacgatat
     8941 tgatcgtatt cgtctctttt tagagaaaaa agaaaaataa tgttacattt tataacatta
     9001 tcaattgacg tttgtctttt tttagactat aatagacaga aagaaggaaa ttgtaaatga
     9061 aggaaaaaga atttcgccga aatatggctg tttttcctat cggcagtgtt atgaagttga
     9121 ccgatttatc ggcgcgtcag attcgttatt atgaagatca agagttgatc aagcccgatc
     9181 gaaacgaagg aaatcgtcgc atgtattcct tgaatgacat ggatcgtctg cttgaaatca
     9241 aagattatat ctctgaaggt tataatatcg ctgccattaa gaaaaaatat gctgaacgtg
     9301 aagcgaaatc caagaaagcg gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac
     9361 tcctccaaca ggggcgtttt gcttcagtac agtcaccttt tggtcgcggt taggcaaccg
     9421 caagtagtca tagattaagg agaaaactta tgccaatcac agctgcagat attcgtcgtg
     9481 aagtcaagga aaaaaatgtt acctttattc gtcttatgtt ctcagatatt ttgggaacca
     9541 tgaaaaacgt cgaaattcct gctacagatg aacagttaga taaggtcttg tcgaacaagg
     9601 ttatgtttga tggatcttct attgaaggtt ttgtacgtat caatgagtcg gatatgtact
     9661 tgtacccgga cttggataca tggacagtct tcccttgggg agatgaaaat ggaagtgttg
     9721 caggtctgat ctgtgatgtc tatacaacag aaggtgaacc atttgcgggt gaccctcgtg
     9781 gtaatttgaa acgagctctt cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg
     9841 gtccagagcc agaattcttc ctatttaagt tggatgaaaa tggggaccca acacttgaag
     9901 tgaatgacaa gggtggctac tttgatttgg cacctactga ccttgcggac aacacacgtc
     9961 gtgagattgt gaatgtcttg accaaaatgg gatttgaagt agaagcgagt caccacgagg
    10021 ttgcggttgg acagcatgag attgacttta agtacgatga agttctccgt gcttgtgata
    10081 agattcaaat ctttaaactt gttgttaaaa ccattgctcg caaacacgga ctttacgcaa
    10141 catttatggc gaagccaaaa tttggtattg ctggatcagg tatgcactgt aatatgtcct
    10201 tgtttgatgc agaaggaaat aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt
    10261 tgtcagaaac agcttaccat ttcctaggcg gtttgatcaa gcatgcttac aactatactg
    10321 ccatcatgaa cccaacagtt aactcataca aacgtttggt tccaggttat gaagcgcctg
    10381 tttacattgc ttgggctggt cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg
    10441 gtatgggaac tcgtcttgag ttgcgttcag tggatccaat ggcgaaccct tacgttgcta
    10501 tggctgttct tttggaagtt ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc
    10561 ctatcgaaga aaatatctac atcatgacag cagaagagcg caaggaagct ggtattacag
    10621 accttccatc aactcttcac aacgctttga aagctttgac agaagatgaa gtggttaaag
    10681 ctgctctcgg agatcacatc tacactagct tccttgaagc caaacgaatc gaatgggcaa
    10741 gttatgcaac cttcgtttca caatgggaaa ttgataatta tttagacctt tactaatact
    10801 aatatagaag aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata
    10861 cctataaccc ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac
    10921 tcttttgcga attagattta tcatcagacg tgtagaagaa aattaaggag tcactatgga
    10981 aaaattcaat ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta
    11041 caagtttact aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga
    11101 tatatttgaa accatagtaa attgagacta gaatagtaca ctacatctgc taaaaatttc
    11161 tagaaactaa tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt
    11221 atctcaataa atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat
    11281 cgagcggaag aatatttctg ccgaggttgc caaggggaaa atcctgaaga tattgaattt
    11341 tatgatgaac aattacaggc agaaaaggtg gaggatttga atattcgatt ggaagtaaaa
    11401 aattgaatta tctacaatct catcaacaat aaacctattt caaccccatt atctataggc
    11461 gtatttttac gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg
    11521 atggaagtac tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga
    11581 ggataaaatc aatttattgg aattttgggc aaaataagcc agagaaatcc tttaggtata
    11641 tagatacgtc tagtattgat agaaaaaaga acataatcaa ctacaaaaat ctacaatatc
    11701 tttcacctga acaagcgcct tcccgtgcta gaaaattagt ttcgcagaat agtgtcttat
    11761 tttcaacagt tagaccatat ctaaaaaata ttgctgtagt tagagaactt aaagagtatt
    11821 tgatagctag tacagcattt attgttttgg atactttact taacgaaaca tatttgaaat
    11881 attatttatt atcagataat tttatcaatc gtgttaataa taaatctact ggaacaagtt
    11941 atcctgcaat caatgattat aattttaatc tattattaat tgctctcccc ccactatccg
    12001 aacaacaacg aata
//
LOCUS       DAVMMR010000056        11114 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMMR010000056 DAVMMR010000000
VERSION     DAVMMR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.056"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(233..1036)
                     /locus_tag="VYS74_001637"
     CDS             complement(233..1036)
                     /locus_tag="VYS74_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV3789643.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1319..2074)
                     /locus_tag="VYS74_001638"
     CDS             complement(1319..2074)
                     /locus_tag="VYS74_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV3789644.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2144..4795)
                     /locus_tag="VYS74_001639"
     CDS             complement(2144..4795)
                     /locus_tag="VYS74_001639"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV3789645.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(4807..5769)
                     /locus_tag="VYS74_001640"
     CDS             complement(4807..5769)
                     /locus_tag="VYS74_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV3789646.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(5771..6328)
                     /locus_tag="VYS74_001641"
     CDS             complement(5771..6328)
                     /locus_tag="VYS74_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789647.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            complement(6315..6764)
                     /locus_tag="VYS74_001642"
     CDS             complement(6315..6764)
                     /locus_tag="VYS74_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV3789648.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7177..7572)
                     /locus_tag="VYS74_001643"
     CDS             complement(7177..7572)
                     /locus_tag="VYS74_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV3789649.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7668..7922)
                     /locus_tag="VYS74_001644"
     CDS             complement(7668..7922)
                     /locus_tag="VYS74_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV3789650.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(7932..9266)
                     /locus_tag="VYS74_001645"
     CDS             complement(7932..9266)
                     /locus_tag="VYS74_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV3789651.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9266..9499)
                     /locus_tag="VYS74_001646"
     CDS             complement(9266..9499)
                     /locus_tag="VYS74_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV3789652.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            9530..9607
                     /locus_tag="VYS74_001647"
                     /pseudo
     CDS             9530..9607
                     /locus_tag="VYS74_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9902..10090)
                     /locus_tag="VYS74_001648"
     CDS             complement(9902..10090)
                     /locus_tag="VYS74_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789653.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(10050..10232)
                     /locus_tag="VYS74_001649"
     CDS             complement(10050..10232)
                     /locus_tag="VYS74_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789654.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(10234..11013)
                     /locus_tag="VYS74_001650"
     CDS             complement(10234..11013)
                     /locus_tag="VYS74_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789655.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT         3257 a         2464 c         2011 g         3382 t
ORIGIN      
        1 aaacttatca agcgcaatgc ctttggtttt cgaaactttg aaaacttcaa aaaaacggat
       61 ttttatcgct ctgaacatca aaaaagaaag gacgaaattt gtcctttctc gaacttagct
      121 tttcttcaac ccactacaat tgacaaagag ccaaactttg atgatatacg ttttcttgtg
      181 agaatattta cttcattttt tcctaaaatt caatgtttac tcagtatttg gattatgaaa
      241 aatcgaggtc taaatctaga tacatttttt ctgaagacaa atcattttga ccaccgagca
      301 agagattttc aaaaaaagct gttaaaaact caggacgtcg ctgtaaaatc tttgcattat
      361 ctaataccaa ggcatcacga aaatacttgg aatgttgctg aaatggtata ttatcaatat
      421 caaaaccaaa ctcacgaaga tactgaatca aaaagaccgt tactgtccga gtgtttcctt
      481 cgcgaaatgg atgaatctgc cagattcctg aaataaaatg ctggatttgt ttaaccacat
      541 ccgcctgagt tagtgtcgca tatgcaactt gtttttcctg attaaaatca taatctaagg
      601 tcatttgaat catggagtaa tcagagtaca caacactttc accattcaaa acaggttcat
      661 tctttgtgat attggtctga cgaaattgcc ccaccggaat agagggttca aatatatctt
      721 gaaacaactc cttatgaata gcaagtaagg tcgcaggact aaagctaaag cctcttcgag
      781 acaatagttc tacaatacgt agagaaacca agtctgcctc ttccgtactt gcatcaatag
      841 tatggtgata agccgttgca tcctcataaa cctgctcata agtcagttct ccccgggact
      901 gtttctcagc caaagattcc atatacgctg atggcactag attgtcaact ttctgcagac
      961 caaaacctat ccgccataaa tcacgcttcg cttcataaga caagtttgga ttgtcaatgt
     1021 tgtaagttgg ttgcatagaa atatcctttc aattgttttt cttcattacc agtaaatcac
     1081 gaaattggtt ggaaactggc ttaaactata aaaagtcctc acaataggtt ctatgatata
     1141 tatatatata tatacttcat gtaaataata aaagagttat aatcagcact actgataaat
     1201 tcaataccga tttcgccatc ttaaaactta gtcaaacgaa taccgttgat ttccagttca
     1261 ttaagtcgtt gacctgtcaa gacttctcgt tccttcaact ggaaaagtta agggggaact
     1321 aatacaaatc ctctacgctt ctatattcaa tgatttcatt tttgataata ttctttttaa
     1381 actcaaaatg ttttaaagga ttatcgatta acactaactt tggaatatca tctagatttg
     1441 tgaccaagga taaaataaaa tccgattgaa actccttgac tttttcaaat tcttttgcag
     1501 ttaaacgcac acgcccctta gattttcgga taccttttac ttcggcaagg taagaatact
     1561 cttggacatc tacctgaaag tcatatccat caccataaag acgggcatcg gttaactgtc
     1621 caccttggaa tttttcttct ttatcaaaat gaaggataaa ataattctca gcctccattc
     1681 ccgtttcctg tagacgtttg aatttagttc gaactattgg tttttcaacg atggttatgc
     1741 ctgttttctt cccttcactg gcaagtaaca ttttaactat ttcagcataa ctatgaacat
     1801 cttcatttcc aaacatcata tcaattaaat ctttacgaaa acgataaact tcagctttct
     1861 gccaccaacc ttttcgatta ttgtcaaaat aaggatcaaa taaatccatt ctatttttta
     1921 caactccagt tgtttcagca attcccagag aaaccacata gcgataaaac tccgacttgc
     1981 taaagcattg aaattctttt ataaaaagct tgtcaaactt ggcaagaccg tagccaatta
     2041 aatttaacaa ttcataatga gggtgtttgg acatcacttt ctccatcaat agatattgat
     2101 ttcattatac caaaaaacac ggctcatcac cgtgtttctg tgtttatttc accaacttct
     2161 tcatctcatc aatacgtgct acggtcacat catacttggc ttggtagtcg gcttgtttgt
     2221 ctttttcttt ttggacaact tctggtttag cgttggcgac gaagcgttcg ttagagagtt
     2281 tcttaccaac catgtccagt tctttttgcc atttagcgag ttctttgtcg agacgagcta
     2341 gttcttcttc aacattgagg agatcggcca gtggcaagaa gatttcggct cctgtaataa
     2401 cgcttgacat agccagttca ggtgcaggga tggttgatgc gatttccaag tgttctggat
     2461 ttgtaaagcg tttgatatag ttgacattgc tgttaaagaa ggcttccaag tcactatcgc
     2521 ttgtcttaac aaggatggtg ataggcttgc ttggtgctac atttacttcc gcacgcgcat
     2581 tccgaacagc acgaattaag tctttgagac tttccacacc agtgtgagcc gcaaggtctt
     2641 caaaggctag attaacagtt gggtatgcag ctgtcacgat agaaccttct gagatttgtc
     2701 caaagatttc ctctgtcacg aatggcatga ttgggtgaag gagacgaagg atcttgtcca
     2761 aagtgtatag tagaacagaa cgtgtgatga ctttctcttc ttcgttatcg ctataaagga
     2821 cttccttggt caactcaaca taccagttgg caaattcttc ccaaatgaag ttgtaaagga
     2881 tatgaccagc cacaccaaac tcgaacttat caaagttttc agtaactttt gcaatggttt
     2941 cgttgagatt gtggagaatc cagcggtccg tcacattacc agcctcacct gttgcaactt
     3001 ttgtgacatt gtcatgcgcc acatccagcg tcaaacctcc attgttcatg aggatatagc
     3061 gagaaatgtt ccaaatcttg ttaataaagt tccatgaagc atccattttc tcataagaga
     3121 aacgcacgtc ttgtcctggc gcagaaccgt ttgaaaggaa ccatcgaagg gcatcggcac
     3181 cgtatttctc gataacatcc attgggtcaa tcccgttacc gagagactta gacatcttgc
     3241 gtccttgctc gtcacgaatg agaccgtgga taaggacgtt ttggaatggt tgacggcctg
     3301 tgaattccaa tgactggaag atcatacgag acacccagaa gaagatgatg tcgtaacctg
     3361 ttaccaaggt tgaagttggg aaataacgtt taaagtcttc tgagtcgact tcaggccagc
     3421 ccatggttga aaatggccag agggcagaac tgaaccaagt atccaagacg tcttcgtcct
     3481 gagtccatcc gtcaccttct ggagcttctt cgccgacata catttcacca tcagcattgt
     3541 accaggcagg gatttggtga ccccaccaaa gctgacgaga gataacccag tcgtggacat
     3601 tttccatcca ttgaaggaag gtatcgttga aacgaggtgg gtagaattcg accttgtcct
     3661 ctgtgtcttg gttggcaatg gcgttcttag ccaattggtc catcttgacg aaccattgag
     3721 tagacaagcg tggctcaact acgacacctg tccgttctga gtgaccgact gaatggacac
     3781 gtttttcgat tttgacaagg gcaccgattt cttccaactt agcaacgact gccttacgag
     3841 cttcaaaacg atccatgcct gaaaattcaa aggcaagctc attcatagtt ccgtcgtcgt
     3901 tcatgacgtt gacttgtggc aagttatgac gttggccaac caagaagtca tttggatcgt
     3961 gggcaggtgt gattttcacg acaccagtac caaactcagg atctgcgtgc tcatctccaa
     4021 cgattgggat gagtttatta gcgattggaa ggatgacgtt tttaccaatc aagtccttgt
     4081 agcgcgggtc ttctggatta accgcaaccg caacgtcccc aaacatagtc tcaggacgag
     4141 ttgtagcaac ttcaagggcg cgtgaaccat cttccagcat gtaattcatg tggtagaagg
     4201 caccttctac atccttgtga atcacctcaa tatcagaaag ggctgtgcga gctgctgggt
     4261 cccagttgat gataaactca ccacgataga tccagccttt cttgtaaagg tccacaaaga
     4321 ccttacgaac agcttttgac aaaccttcgt caagagtgaa acgctcacga gaatagtcta
     4381 cagaaagccc catcttgccc cattgttcct tgatggtagt ggcatattcg tctttccatt
     4441 cccagacctt cgtcaagaaa gactcacgac ctaggtcata acgcgtaata ccctcaccac
     4501 gtaagcgctc ctcaacctta gcctgagtcg caataccagc gtggtccata cctggaagcc
     4561 aaagggtatc aaagccctgc atgcgttttt gacggatgat gatatcctgc aaagtcgtat
     4621 cccaagcgtg accaaggtga agtttcccag ttacgtttgg tggtggaatc acgattgaat
     4681 aaggcttagc cttttgatcg cctgaaggct tgaaaacatc cgcatcaagc catttttggt
     4741 aacgaccagc ctcaacctcg gctggattgt atttagatga aagttcttta gacatgtgtg
     4801 ttctccttaa ttacttttat tgacaatttt aaccaaacca atttttgaaa gttcaatata
     4861 aatctcttca atttcagcaa atgctgcaag tggtgacata gtataatttc caggattata
     4921 tttaagatca tacttgctta tctttgaaat ataataccta gagtgtttca attttaatga
     4981 ttccaacttt gatttaatat ctactaatgt attatggttt aactttaaaa acaacttatt
     5041 ataattacca tagagctctg taagagcatt aaagtattct ttcactagag ctatagcgtc
     5101 aatcttagat gtttcttggt ttagttttat caactcccct ttcaattttt tccatttaaa
     5161 gttggaattt aaaagataat ctaaatcaat atagaaatct attataacag tctcatcatc
     5221 tgggctaaca gcttttacct catttattgg taagtcagta tgttgcgaat aatttctcaa
     5281 agaatggcaa aatcgataag caaaattggt atcatactga tagcttgcaa aaccatgtat
     5341 acaatcaaac tcctcactat ctttggaata tttttgttta atttggtttt cgttaaaatc
     5401 gacaaacaat tttccactgg ataataaatg aatcatgaaa cgatttacat ctgcctcttc
     5461 aatttctaat tcctttgact caagtttatt tttaatactt aaaaaatccg agacatctga
     5521 aataaacaat tcgaacatat tcttgatttt ttgaaaattc ttttgaatct caaaaatttg
     5581 gtccgagact gacattattt ccttggttgg ttcaatatca gagatgacaa tacgtttttc
     5641 attgttgtta atttctaaat aagctaatcg ctcgataata tcactatcat ttaattcagc
     5701 atactcaaca tcaatttggc gtaactgctt ctcagataga gagcttactt cctcttttac
     5761 atcgctcatc ttattcctcc cactcgctct tcagcaagcc atatatcaga ctgtcacagc
     5821 agttgccttg agcatctttg cggtctctta tgcgagcttc gagggtaaag ccaagctttt
     5881 ccgcgactcg tttactttga aggttatatc caaagcaagt tagttcaatc ttgtgaagac
     5941 ccaaatcttt aaaggctaag tcaatcaagg cacgcgcagc ttctggcaca taacctcgcc
     6001 cccaatagtc tgggtgtaag gtatagccga tttccagcac atcatcttca tggcgatggt
     6061 tgaaatcgac agagccaacg atcttatccg ttcctttgac gacaatcccg tagcctgctg
     6121 ggagattttc cttttgatta cgctccggaa gaatgtgctc cagataataa atctcatctt
     6181 ccaaggtctt gactggagga aaacctgctg gataggcgac ctctggcaaa ctagcatagt
     6241 caaagatatc ctcagcatcc gccacagtac ggactcgtaa aacgagacgt tctgtttcga
     6301 tttgatctgg cagctcagtt cttgccatcc ttcttcctcg cttttttgat gaaactgccc
     6361 ttcatatcta cacgcttatc cagatagcga taaacgcgct gatatccatc tcccatgaaa
     6421 taggttgggg caaacagttg atttttaaaa tgtccctttt catccaggag ttctggggca
     6481 acaagtcgct caagaatctt ggcaaagatg tggcaaatac cgtcttcctc aacaatccta
     6541 tctaccctac aatctaaaac aagtggacag gcgtctaaaa taggagtctg agttcgttca
     6601 gaaatttcat aatgcactcc caaacgttcc aatttctcct gatgactgat aaaaccagcc
     6661 tgctccatcg caagcataga agtttcatca gaaatattca cagtaaattt ttgatactgt
     6721 ttgatctgct ctgcggcatt ctctctcgca acgactccaa tcacaaccca atctcctaga
     6781 ctataagagg aactacaggt cgtgatgtta tagccaaaat tctaatcttg atatcctaaa
     6841 ataaaaacag gaaaaccata atatagttta cttgtgttaa aagattgctt cataacaacc
     6901 ccctttgact aagacgtaaa agaaaagccc tgccatctac atgacaggga cgaatgtgtt
     6961 tatccgcggt accacccaat ttcgggcagt tgcccgcaac tctcgtcttt aaagcaaaaa
     7021 gacactttta tttcattttt tcagtcatta gcaaccagtt ttgacacatt tgtggacttc
     7081 tcagcgccgc cactttctgt aaaatgtggg attcaaaaca cctctaatgg ctctattgta
     7141 gcaagttttt ctcggaaatg caaggctcaa gaaaaattac ttgaacttga tgccattttc
     7201 tttcaatttc ttgatggtag ctgggccgat tcctttcaag gcaaggagtt ctttttcagt
     7261 ccagtttttg aaatctgaag cagacttgat tccttcatca tagaaagtct tggcacgatc
     7321 aagtggaagt ccacccaagt ttgctgcaaa atcttctaca gaattagcta ctttttgagc
     7381 ttgctctttg gtagcttcta ctgcttgttc aactttttta gaaacagcct taccagctgc
     7441 gcttgctgac tgttctgcac gcttcacgac tttttttgtc tcatctacaa ccttagtcac
     7501 agtacttgaa aaggcacctg cgcgacggag gccatttcgt aattgttttt tacgattgag
     7561 tttctttgac atgataaaat cctttcaata aaaaatccct gctctgacaa ggatttaaca
     7621 taaacattct atcaagattt tagtgaaaaa tcaagaatct ttctcgttta ataatttcgc
     7681 tcaccaaaca agccttctgg caaatcatgg aatcccttgc ctgctttgaa gttttcaaac
     7741 ttaccaagca agaactgata agcatccaag cgactctcgt agcgaatcat ggcatctttg
     7801 gcacgctcgt cctggtcttc ttcgtaaact ttttgacttt ccttgtgcgc catgtcgtta
     7861 atcatgattt gggtattgac ccaagcttca aattccttca taaattcttg ttcgtaactc
     7921 attttttctc cttattctaa tccacgaaaa tagtccgtat caatctcacg gtagggctgg
     7981 atatcctgaa catactccaa cactccttgg aattctccgt cttcatcgtg tactgcagca
     8041 taggtgatgt ggacaaactt acctcgcgac tcagacttga accacatttc atacttgtcc
     8101 ttggtcccct cacgaagccc cttcatgata gttttgacct tgtccaagta cttaggcgga
     8161 tggcagagtt cgacattgcg cccgacttga gacggcgtcc gtttgaaaat catctcatca
     8221 gctggcgtat tgtcattgta atactggaaa atatcatctt tattgacaaa ggtaatctcc
     8281 atagggaggt gattgaggat gagattggcc tgctcgactg aaagatagcc attaccaaaa
     8341 gcctgttgac tatggcggtc cagcacagct tccttttcct taggggtaaa ggtaatggta
     8401 aaatggcctt ctggcgtatc tatgacttgt tgaacttgac cttctgccgt atctagctgt
     8461 acaggctcct ctgcaatctt ttcctcaata aagctctgtc gttctggcac ccatttctct
     8521 gacggacgga tgatggcata gccataggca tcgctctcct ccgcaatctg aagccagtca
     8581 tcctgagtaa aagactcaag gagaatcatg aggaggatgg actcttcctt gaaaatcata
     8641 ctttcaaact ctgtcgcaaa agcttcaaaa gcttccttta cactgctaat tgacacttct
     8701 ggtagtgact tggctatcgt tagagctgtt tgaaagagtt ccctaatctg atcatccact
     8761 ccccacataa ctttgggagg tgaatcgtgt ccatagcgct ccatgatagg aaagaagagt
     8821 tcttccttac gttggtaatg gatgtcaaat tgacccacaa gtcccatctg acgcaccaaa
     8881 cccttacgca tctccgccag catttcctcg tcttccatag actcataggt atctaacaat
     8941 ctacgaatgc gaatcaaggc cgcacggaga gccagatttt cttctttgaa gacacgaact
     9001 gggtgacctg gatgctcagt atctgaaact tcgacacctt tgatagcatt tttaaaaaga
     9061 ttggcatgga catcacagag ttccataaca tcttcaaagg tgacacctga gtctgagttc
     9121 atcagctcgt gctccataag ggaaatctca atggctgaga cacccgcaaa ggtcgcatca
     9181 aaacgctcct gaaccgactc aggagaggcg ccattatgca attctaataa aatatcccgt
     9241 aggacatgaa tccgttcatc tgccattagt ctaatccaat cacttcgtag ccattcgctt
     9301 ccagtgtgcg tacaatcttg tccataggag ttcctgctag cttagaacct tgtttaagtg
     9361 atactttacg accaactgta ttgcgcatca agggattggc aaggggttta aaacccagct
     9421 ccactagaat ttccaagact tctggatgct tgtccaccac ttctgcaaca ggaattgaca
     9481 catcgatgat attgtccatg acgacctcca ttatatgttc taatactcaa tgaaaatcaa
     9541 agagcaaact agaaagctag ccgcaggttg ctcaaaacag tgttttgagg ttgtagatag
     9601 aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg gatagaactg acgaagtcag
     9661 ctcaaaacac cgttttgagg ttgtggatag aactgacgaa gtcagctcaa aacaccgttt
     9721 tgaggttgtg gatagaactg acgaagtcag taacatatat actgcaaggt gaagctgacg
     9781 tggtttgaag agattttcga agagtataaa atgattttac tgcttatatt ataccatatg
     9841 gagagagatt aaaaaactag cagtggagtt cctgctagct ctctgcgact tggattacca
     9901 tctagataag tttaaagagc caatccaata tcttaaataa tatcttgtaa aacagcaatt
     9961 gaactgcaaa ggaaatgatc atccaaaaga atttcacaat cccaataatc ggtttgataa
    10021 aaagaatagc aagaagacca aaaaaaattt cattctctct cctctggcct aatgagccac
    10081 cgagccgtat ccattagctt atctgcaata aaaagttttc ccagaccaac aacgacatgg
    10141 tcagctttct ttatcgtttt catcactttt acaccttgca cggtcaaaaa gtaattacca
    10201 taaattttat caaaagcttt gataggattc atattactct cctttaatga tttcagcctc
    10261 ttttcggatt gtttcaacat cttcttgata ttgaacttca ctatagttga ctagcttgga
    10321 taattctttc tgcaagcttt cccccattgg tttcatagtt gcaaaggtta aaccacctgc
    10381 aatgatacca cccaagatag gaataaattt ccctattcct ttggccagcc ctcccttggt
    10441 aagattcaca ccaaatattt ttaagacttt tttcaaaata gggtaccaaa gcgtctttgt
    10501 taaagcttta ttaggcactg ttttcattac ctgtttggca attgttatac caccagcaca
    10561 tagcaaagca gcggttccat tcacccctaa cattacgcct agataaagca agagggtatt
    10621 ttgagcatct tcactcaact cctcgcgtga agcccaaaga tcctcataac cataaatata
    10681 acctaattct tgagccaatt tcagagagaa agcataaaat tgagccacat cagctggaat
    10741 ggtaattgcc atagcaagcc ctccagctaa tcctgccaaa acagaagtcc cactcgctaa
    10801 taatacattg tcccgaatac aagcattagc tacacgatct aatatttctt gagatagaag
    10861 agtcgttgta ccttgttcca ataaggtagg aatatctttt ggatccaatt ctttggaaaa
    10921 cttatccact aaaaatttcg aacgatcaac cttaacaaca ggtagtttca ccacttgttg
    10981 caatacttgc atagctaagt cttgttcagc catatacaaa ctccttattt cttttttaca
    11041 ccttatcata ttatatttcc tacaaaaaaa gcattttcta attagataac attaaaaaat
    11101 acagaacccc tttt
//
LOCUS       DAVMMR010000057        11068 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMMR010000057 DAVMMR010000000
VERSION     DAVMMR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.057"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(256..1059)
                     /locus_tag="VYS74_001651"
     CDS             complement(256..1059)
                     /locus_tag="VYS74_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV3789656.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1143..1364)
                     /locus_tag="VYS74_001652"
     CDS             complement(1143..1364)
                     /locus_tag="VYS74_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789657.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(1843..2052)
                     /locus_tag="VYS74_001653"
     CDS             complement(1843..2052)
                     /locus_tag="VYS74_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789658.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            2222..2494
                     /gene="rpsP"
                     /locus_tag="VYS74_001654"
     CDS             2222..2494
                     /gene="rpsP"
                     /locus_tag="VYS74_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV3789659.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            2514..2753
                     /locus_tag="VYS74_001655"
     CDS             2514..2753
                     /locus_tag="VYS74_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV3789660.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            3021..3225
                     /locus_tag="VYS74_001656"
                     /pseudo
     CDS             3021..3225
                     /locus_tag="VYS74_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            3329..4117
                     /locus_tag="VYS74_001657"
     CDS             3329..4117
                     /locus_tag="VYS74_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3789661.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            4148..4666
                     /gene="rimM"
                     /locus_tag="VYS74_001658"
     CDS             4148..4666
                     /gene="rimM"
                     /locus_tag="VYS74_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV3789662.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            4656..5375
                     /gene="trmD"
                     /locus_tag="VYS74_001659"
     CDS             4656..5375
                     /gene="trmD"
                     /locus_tag="VYS74_001659"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV3789663.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGSQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKILVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            5387..5725
                     /locus_tag="VYS74_001660"
     CDS             5387..5725
                     /locus_tag="VYS74_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789664.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(5756..6076)
                     /locus_tag="VYS74_001661"
     CDS             complement(5756..6076)
                     /locus_tag="VYS74_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789665.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            6174..6389
                     /locus_tag="VYS74_001662"
     CDS             6174..6389
                     /locus_tag="VYS74_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV3789666.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(6593..7129)
                     /locus_tag="VYS74_001663"
     CDS             complement(6593..7129)
                     /locus_tag="VYS74_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV3789667.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7555..7893
                     /locus_tag="VYS74_001664"
     CDS             7555..7893
                     /locus_tag="VYS74_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789668.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            7959..9941
                     /locus_tag="VYS74_001665"
     CDS             7959..9941
                     /locus_tag="VYS74_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789669.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            9938..10561
                     /locus_tag="VYS74_001666"
     CDS             9938..10561
                     /locus_tag="VYS74_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3789670.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            10558..11058
                     /locus_tag="VYS74_001667"
     CDS             10558..11058
                     /locus_tag="VYS74_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789671.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
BASE COUNT         3734 a         1737 c         2187 g         3410 t
ORIGIN      
        1 ttgtgggtat ccatattgat gatatctaga atcttgatgt ttgggtcttt aatatcgagc
       61 agttttgtga taaaatgtaa ttgttccata tgaatctttc taatgagttg ttttgtcgct
      121 tttcattata ggtcatatgg gacttttttt ctacaataaa ataggctcca taatatctat
      181 agtggattta cccactatag atattataga gccagaaata cagtaccgaa tgtggatact
      241 gtattttttt ggtttttaag atttaaaatc gtagtctttc accacttcga tgctgtcgat
      301 tgtgatagca gtagttggct tgtctttttc atctttttcg gctttagcaa tcttatccac
      361 aacatccata ccgtcaatca cttgaccaaa gactgggtgt ttgccatcta gactagggtt
      421 tccaccttct ttgtaggctt cgataatttt ctgtggatac ttgcttgtag ggagtttaga
      481 agaggtatct gtagagtttt ggttgatgaa gaactggctg ccattggtgt ttggttgacc
      541 agtattagcc atagcaagag caccacggat gttatacaaa taaggagtaa tctcgttctt
      601 gaaaccagtt cctttgtctt tagtcttatc cttgtcatgc catatggact gaccacctgt
      661 accgtcccct tttggatctc cagtttggac cataaagcca tcgatgacac ggtggaaggt
      721 aataccgtta tagtagcctt ctttggcgtg agtgaggaaa ttttcaaccg ctagaggagc
      781 gagtttaggg aagagtttaa tgcgaatatc accttggctt gtgtggagaa tcacttcggc
      841 ttcatcttca gcaacttcct tagatagttg tgggaaattg gcgttttcgt ttgttaaagc
      901 atcgtttaac tccttggcag attgggcagc tactttggaa ctttcttcag cagcaagact
      961 ggaatcaaca taatcatcac cacgcagact gcgttggacg ctgctacacc cagctagggc
     1021 tacagtagac agtaaaagaa gggttgctag ttttttcatt ctgatcctct caaaaattca
     1081 tttataacca ttgtacccta aaaggaagtg gatttcaaat attagtagtt tacagaactg
     1141 gcttagataa tagttgggat ttcatcatca tcttgaaaat cttcaacttc ttcaacttct
     1201 tccccagtaa atggtttctg aaaagaactt agtataaaac gggcaattgg ttcaacaatt
     1261 aggaaattta gaggaagagc tgcaataaag tttacgaacc aggcgcttag ataggtattg
     1321 ggtgtgatag caatgccatt gtaaaaaagt ccatagaagg acatgaaagt gaccagctaa
     1381 ttcttcttct gaaaaatgct catgaagggc ttgatccagg tcgcaagatt tcattttaga
     1441 aagtccagta actcccttct tcttcaggat attctttttc atggtagtat ttagatggtc
     1501 aagtgagtca aaagaagttt cgacaagagc ataccctttt tctaccagag tttttagatg
     1561 tttaggggca tcaataccat aagtgtactc aaagtatttt ggaaaccaga tcttaagaaa
     1621 gctcgtaaag ctagccagtt ctctaaacgc taagaactag ctacaacaca gcatttacaa
     1681 cacttcatgg ttcacaccat ggggtgtttt ttaatgattt taagcaaaat tcacacttaa
     1741 ttgaaagtta tttaaattag atgaaaattt attttttgaa aaagtgggaa aacattgatt
     1801 ctaagcgaat tttgaatcat attcgaatgg gagtttttaa aatcatgttt cagtggtaaa
     1861 agtaccaaac tggattctcc atagtaaaat gatatctcct gctagtgaat catctactaa
     1921 taagtccata tttcgcttgg gaacgggctt gggacttaga agccaagtat ccaactctcg
     1981 ttcaggcgat atgaagggtt tgacttggtg ttctttataa aatttttcta aaatagtatt
     2041 cacggatagc atctttcaaa catttatttc ctattctaac aaatttctaa atactttaag
     2101 aaatttatag tctgtcaagt ttttttcttg acacatgcca gaaatctgct ataatagaac
     2161 atgtgctaga tagctcagct atttcaccga aataaaaaat taaagaaatg agactttaaa
     2221 aatggcagtt aaaatccgtt tgactcgtat gggttctaag aaaaaacctt tctaccgtat
     2281 caacgtagca gattcacgtt caccacgtga cggacgtttc atcgaaacag ttggaactta
     2341 caacccactt gttgctgaaa accaagtaac tttgaaagaa gaccgcgttc ttgcatggtt
     2401 ggctaatgga gctcaacctt cagacacagt acgtaacatc ctttcaaaag aaggcgtatt
     2461 gaaaaaattc cacgattcta aattttcaaa ataagtttaa agtaggttga cagatggata
     2521 cgattgaaaa tctcattatt gcgattgtga aacccttaat ttcacaacca gatgccttaa
     2581 ctatcaagat tgaggataca ccagaatttt tggaatatca tttgaatctt gatcaaagcg
     2641 atgtgggtcg tgtaatcggt cgtaagggtc gcactatttc tgcgataaga acgattgtct
     2701 actctgtccc aactgaatac aaaaaagtaa gaatcgttat tgacgaaaaa taagaagggc
     2761 gggacggatg tctcgctttt ttgtgtggaa tagaagatat agtaaaatga aataataaca
     2821 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag taaaggtgta
     2881 ctattctagt ttcaatatac tataaaagat tgaacgttta ggcaattcca agactactta
     2941 ctcgaacatt aacagcagtc tcggactgat gaaggtctct ttatgaagtt agtggagaag
     3001 tcggagaagt agccgaggtt ttgaataggc gctctggtcg aaaagaaggg ataggattcg
     3061 aacgaggaac ttgccagaga actggctgat atcatttact acaccgtcgc aatcgtagct
     3121 attaatcata ttgaccttac caagactgtc tttgagaaag acaaaaccgc agccgttaat
     3181 taccaacata aacatgattt ggaaggattt ttgaagggaa agtagagatg ttgtcccctt
     3241 cctccaactt gagaaaatcc ttggaacgtg ataaaataaa agataaggat ttttaataat
     3301 tcattatata gaatgagtgg atttctttat gaagaggttt gaagtatcta cagaaattgg
     3361 tagcctctct gttacttatc aaaagcaaaa gaaagtgcta gtttgtctaa acggagcagg
     3421 tttgctacca agttatgaaa atttttcact tatacttgaa aaacctcctc ctacaattgg
     3481 ttatttgaca attgattttc cgaacacagg taggagtccg attcatgacc aagctggaaa
     3541 aaatctagat aatcttgcag atgtggttta tgaagtactt gaagaattag ggatttctga
     3601 atatatactt tgtgcacata gttggagtgg aattttagct tgcaaattac tcgagaaacc
     3661 aattaagtgt cagactttag tagcaattga accgacaact aaaaaagtca tgtttgctga
     3721 tttttcagaa aatccttatc cagaaatgga agagcagatg aggctgattg acgagtgtgg
     3781 tcctgaactt tattttaaga acttaactca agcaacattt agtcctgaaa cgaataaaaa
     3841 aatctgggaa ttaatgcaag aaaaaggctt agagttggaa aatcaagatc cagaatttca
     3901 gatatctgga gagattactg aggaagattt tgagaatttg tcgatagaat ctcatgtccc
     3961 tgtatttatt ttttgtcaga cttatagaga aaaagagtac agagaatcag aatattggac
     4021 ttctaatact aaactcattt taggagggaa tcaccattat ttacaatggt cagaatcgga
     4081 aaaaattgcg gctattattc gagaattgtc agaataagat ggaaaaaagg agattacagg
     4141 agacaagatg aactacttta atgttgggaa aatcgttaat acgcagggat tacagggtga
     4201 gatgcgagtc ttgtctgtga cggattttgc agaagaacgg tttaaaaaag gagctgagct
     4261 ggctttgttt gatgaaaaag atcagtttgt ccaaacagtg accatcgcta gccaccgtaa
     4321 acagaagaac tttgacatta ttaaattcaa agatatgtac catatcaata ctatcgaaaa
     4381 gtacaaggga tatagtctca aggtcgctga ggaagatttg aatgacctag acgatggtga
     4441 attttactat cacgagatta tcggtttgga agtctatgag ggtgatagct tggttggaac
     4501 catcaaggaa atcctgcaac caggtgctaa tgatgtctgg gtggtcaaac gaaaaggcaa
     4561 acgtgatttg cttttacctt atatcccacc agtggttctc aatgttgata ttccaaataa
     4621 acgggtcgat gtggaaatct tagaagggtt agacgatgaa gattgatatt ttaaccctct
     4681 ttccagagat gttttctcca ctggagcact caatcgttgg aaaggctcga gaaaaagggc
     4741 tcttggatat ccagtatcat aattttcgag aaaatgctga aaaggcccgt catgtagatg
     4801 atgagcccta cggaggcagt caaggcatgt tgctcagagc acaacctatt ttcgattcct
     4861 ttgatgctat tgaaaagaaa aatccgcgcg ttattctcct cgatcctgct ggaaagcagt
     4921 ttgatcaggc ttatgctgaa gatttggctc aagaggaaga gctaatcttt atctgtgggc
     4981 actatgaggg ttatgatgag cgcattaaga tcttggtaac agatgagatt tccctaggcg
     5041 actatgtcct cactggtgga gaattggcag ctatgaccat gattgatgct acagttcgcc
     5101 tgattccaga agtgattggc aaggagtcta gccaccaaga tgatagtttt tcttcaggtc
     5161 ttttagaata tcctcagtac acacgtccct atgattatcg aggcatggtc gtgccagatg
     5221 tattgatgag tggccaccat gaaaagattc gtcagtggcg attgtacgag agtttaaaga
     5281 aaacctacga gcgcagacca gatttacttg aacattatca actgacagta gaagaagaaa
     5341 aaatgctggc agaaatcaaa gaaaacaaag aataaaggag aaacctatgc aagtaatcaa
     5401 acgtaatggc gaaattgctg aatttaatcc agataagatt taccaagcca tcttgaaggc
     5461 agcccaaact gtttatgttt tgacagatga tttgcgtcaa aatcttgctc aagtcactaa
     5521 gaaggtagtt ttggatttac aagaagccaa ggtggaacgt gcgactatca gtatgattca
     5581 atctatggtt gaacatcgtt tattgggcgc aggttacatt accattgcag aacactacat
     5641 ttcctatcgt ttacaacgtg acttggaaag aagtggttat ggagatcata ttgcagttca
     5701 tttacatttt gaacaaattc gctaagaaaa aagagtggga tgaagcaact acatctcact
     5761 ctttcttaaa tctatttttt tgggctgttt ggacagttga ggggacaaat cgcaggcgtt
     5821 gaatatcgct tatacggata atccggctca catttttggc aaaatttttc acgataattt
     5881 gctggccttg ctgatcatat ttgatgatat cacctgtaaa acttgtctca gacaagataa
     5941 ggtgaacagc ggtttgtttc tggatagcct cttcaatagt ggctgtaagg gagttggttt
     6001 cagtctggtc aggttggtca tgtccattta aaaaatcatg tattttatct agaacttgct
     6061 ggaatttatg tctcatattg gcctcccttc ttttatatca atattatata aaattttcct
     6121 aaaatctaca agattttacg aataaactaa tgaaactaaa aaaggagaag aaaatggcag
     6181 aatttacatt tgaaatcgaa gagcacttgt tgactctttc tgaaaacgaa aaaggttgga
     6241 ccaaggaaat taaccgtgtg agctttaatg gtgcccctgc aaagtttgat attcgtgctt
     6301 ggagtccaga ccatactaaa atgggcaaag ggattactct ctcaaatgaa gaatttcaaa
     6361 cgatggtgga tgcctttaaa ggcaactaat actcttcgaa aatctcttca aaccgcatca
     6421 gctttgcctt gccgtatgta tggttactga cttcgtcagt ttcatccaca acctcaaaac
     6481 agtgttttga gcagcctgcg gctagcttcc tagtttgctc tttgattttc attgagtata
     6541 agttttcaaa ataaaaaaag gatattgagt catgataact cggtatcctt ttttagttag
     6601 taaagtaagc ctgattttta aggcgttgaa gtaggggacg gctaataaaa ctaatagcca
     6661 gaagtttgcc aaggtctgga atgataaagg gaagaacccc cacaacaaga gctttttcaa
     6721 atgccattcc agctaggaaa tgcaagctga gaatcccgcc gacaaagaca agggcatcac
     6781 ccaagaggtt tgcaagaaaa atcttaacaa caccactctt gctgttggtt agagaggaag
     6841 taagtccaga gtaaacgaga taaaaccaaa gatagcctgc agtagggcca actaaagcct
     6901 gaaaaccagc tccacctcct gcaaagacag gaagaccgat agcacctaga agaagataga
     6961 gtccagcaga aagtacagcc tctctcggtc taaagacagt agcaatcaag ccgattgcaa
     7021 agttttgcag agtgaaggga acaggtccaa ttggaagact gatttgtgcc aaaacagcaa
     7081 tgagagcagc cccaatagca gggatagcat aaacgtgagc ttttttcaaa atagttaacc
     7141 tcttttctaa tgtatagtaa ctattatact catataggag tcgatgtcaa ccatttttta
     7201 aaaacaaggt aacaaaaact gtaacagccg attttcgtga ctagtcaagt tggataattt
     7261 ttttgttgat tcgaaaattg tctgtataga taatcctatt ttattaataa aaaatatact
     7321 agtaaatact tgacaaatta aaaaataagt gttacattaa aaatgtaaac ggatacattt
     7381 agatgtgttc aattttaaaa ggaggggtag ttatgagata ttttaacgac tctagatacc
     7441 ataacggtga ttaaaaacct aaatataatc aattcatatt aaagtaatat ggagggacta
     7501 aaaagagact attatttaat cttcatcaaa tatttaaatt caaggagaag taaaatgaaa
     7561 aaaagaatta ataagaaagc atctgttttt ctagttgcaa gtgttttcag ttttagcttt
     7621 gctactacag ctagtgtcgt taacgcagta acggcacacc ctgctccaag tcccattatt
     7681 gatgtatggg aatatggtgt aaatgataac tatggttatt cgaattactt tgttagttct
     7741 ccaagtaata ttggatcaaa atcttcagta acgaattttt ggggaacagt taaagctcag
     7801 gataaaaaag attatggatg ggcatattca agtgcaacaa aatcttggaa cgatgttcgc
     7861 ttaaatgcat attgggatta ttatagattt taaaaataat ttatgaaaac aagagaccca
     7921 attataagat tgggtctctt gtaatatggt aattgcttat gaaattaaaa ttatgtatca
     7981 ttggattctt cttttgtttg attgctacaa ttggtctggt cactattagt gataccgaaa
     8041 tccccatacc tttaccaatt gatggagctt tttctatcca aggaaaaagt aatctttcta
     8101 ataacgaaat atacgaaatg gttcgtgact tatcaaaaac ggaaaaggtt accatttaca
     8161 agccaatcgt tcagagttcg ggtcagttga agtatgtaaa ttttgatgat gtaaataatg
     8221 aacaattaaa gtcagcacca atagtaggga tgtattacac acttgggaaa atggatgtag
     8281 acagtttgaa accacttacg atgacgggac tacaaacagt ctatatggct tatccttggt
     8341 atataggagg aatattacaa ttcactggaa ctttaagaat actgttaatg ggttcaattt
     8401 atttaacttt attagttgtt ttatttgttg ttaggacgag gcagatcaaa gaaggagtga
     8461 tacgacgttc tttgggcttg cctatatacg acttaagaag agagtatggc atttctctta
     8521 tttttgaact gattatgatg gctttattga tgatttctta cagttctttt ttgggtaatg
     8581 gtttcttcac ctatagttct aagttatttt tctctctgct tttaacgaat tttatactat
     8641 ttcaaatcat tgatcttatt acttttgttt tattttggtt gacgattcag attgaaaagc
     8701 ctattgaaat tattaaaaac aaggcaaaaa acaagcttat ttttgtcgta tggcttgcta
     8761 ttatttcgat tatcatcctt gtctcaggag tttttttaca agaaacaaag agtagtcaat
     8821 ctagtattaa catacagata cagaatttag taccatggga tacagtaaaa gactggagaa
     8881 ggattgagtt ccttggaatt gaaagtaact ctactaaaaa tagagaagtt aatgattcag
     8941 atggtcagta tctacagata gttgctgcct taaaaaactt agatttctta tatatagaac
     9001 ggtcttcagc atatgttcca gatttcatga aaacttcaca tgttatagaa aatttttcta
     9061 aacaattaga aaatgacggg ataacgaatc cagaaataaa taaagagtta atttatatca
     9121 accaaacagg tgctaaccta caaaataaag tgaatggaac aaactatcat cttttagata
     9181 ataagatagc aaccatctat attcctgaga agtggaaaga aaaccagaaa tctattgaga
     9241 atacagttgt agcagaacaa tttattggaa caaactatac aagagaacag cttgcagttc
     9301 aaataattcc tgatggggaa aaaatttttt attttaatga ggatgctgat aataatctta
     9361 aaatgaaaga tattttaccg ctggcaaatg tagcagatag caaagataat atagttgttg
     9421 tgttagatac ggacaaaatg atggaaaata ataagttttc tttggctagt aacatactat
     9481 ataaatctct ttttagtcct gaagcggtaa aaaaaataaa tgagatgact gtcctgttga
     9541 atttctcaat gaatccagta gatgtttacc aaattgtgaa attaaagatt caatccttag
     9601 agcaccaaat tttactttca caaatcttgc agaagataat ttacagtatt gtctttatac
     9661 tcatttatca atatgtccaa ctttttatta ctctaaaaca gaatgaatat gtgaaaaaaa
     9721 tcattttggg tctgtcaaaa acatatatag caatttcaag tctcaaatat tttatgatga
     9781 ctattacaat ggttattcta tttacatttc ttatgacagg gcaaatagag ttgctatata
     9841 ttggagcggc ttctctgcta gttttgatgt tgtcaatcat aatgagtttt agaaaattat
     9901 ctgaaagcta cactaaaatt ttaaaaggag atgaatcatg acaattgacc ttttgaacgt
     9961 ttcaaaaagt ttcggctcaa agaagatctt tacagactta aatttaatat ttgaatctgg
    10021 aaaaagctat gcattgattg gaggttctgg ctccggtaaa agtacccttc ttaatattat
    10081 aggaagatta gaaaaaattg acagtgggaa tgttttagtt gataaacaag atatttggaa
    10141 gataaaagaa agaacttttt ttaaaaatac tgttggatat gtatttcaga attattcttt
    10201 aatagataac aaaacagtgt atgataatct gagccttatc actaaagaca aaaaaacaat
    10261 aactgacgta cttgaaaaag taggactgtc aagtgactat ttacaccaaa aaatatatga
    10321 attgtctgga gggcaggctc aacgtgtagc tattgccaga atgttaatga aaccacgtaa
    10381 aattatctta gcagatgaac ctacaggagc tttagatggt gagattggaa aagaaattat
    10441 tcgtttacta ttgaatgaaa cagctgaaga taaatatgtc attatagcga cacatgatcc
    10501 agctgtctat aacgaagttg atgtgataat tgacatgaaa gatatagggt ataaggtatg
    10561 aaaaagaaaa tttatattgc tttgatcttt gtaactgggg tactcgccat tttctttttt
    10621 ggaaaacaaa tgattacaaa agaaaatatc aataagccaa cggtagagtt aacaatctat
    10681 acgctttctt ctagtgatac agaaaaatgg aataaagtga gacaagtaga aacggaagaa
    10741 gctatatact tcataactgt gaaagaagta tcatcttcag aagaagtatt ttcaaacatc
    10801 attgcaaatg gagctgctac ggggttcgga gttcgtgaag aagaagtgaa gaaattcaat
    10861 aatggtttag gagacaccat agaggactct aagcataata aactaattga aatagaattt
    10921 tttacttttt ctgatgatgg cgcgggattt gtggtagcaa actttgacta cggtaaagaa
    10981 gagttgaatt ctcagaaaaa agatataaaa gaattatata aaaaaatata tgaatctttt
    11041 aaagaaaaga ataaatgatt gtattata
//
LOCUS       DAVMMR010000058        10758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMMR010000058 DAVMMR010000000
VERSION     DAVMMR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.058"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(117..1541)
                     /locus_tag="VYS74_001668"
     CDS             complement(117..1541)
                     /locus_tag="VYS74_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3789672.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VYS74_001669"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VYS74_001669"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV3789673.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VYS74_001670"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VYS74_001670"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV3789674.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VYS74_001671"
     CDS             complement(6528..7706)
                     /locus_tag="VYS74_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3789675.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VYS74_001672"
     CDS             complement(7786..8649)
                     /locus_tag="VYS74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3789676.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VYS74_001673"
     CDS             complement(8652..10553)
                     /locus_tag="VYS74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3789677.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3235 a         2368 c         1910 g         3245 t
ORIGIN      
        1 tgtactattc tggtttcaat ctattataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctggttt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag aagattgccc
      481 tcacgtttga ttggtgtaag agcctgagct tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg taaataagcc gcacgttcaa ctgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaaacttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacgtcaagg cgaggctaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctactcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt actaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaaccac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggtag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatat gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagcc ttacctacca aatcttcctc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatggtca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaat actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 agacagattt tcaattgaat ggaaatattg aagtaaaagc tgactaacct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 ggcagggaac ttgtaggcta ctgcccactt tggagcctta actgtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcacttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgcaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctcttccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat attgagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgcatggcc ttttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaatcaaaac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taagtcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa agggaagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatc tttacacctg atctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggcc ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caaaacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttggtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtagcta gcttttgctc cttcaactca acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat acttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggag
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tagctagagc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattt tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgttta
    10261 tctgtccgac gtaagtcatc aaagacatga agcatctcat catagatggt attttcagac
    10321 tcaaaacggc tatcttgagc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccactagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat catacaagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag tcttttagta caacttttat
    10621 aacataaaat aaactaaatt atgtatattt tatattagat tccttcacta tcttgttgga
    10681 ttttctaacc agctaatctt gtttcaaata gttatcgcac aagcctatta tttaattctt
    10741 ttcattattt acgtacgc
//
LOCUS       DAVMMR010000059        10662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMMR010000059 DAVMMR010000000
VERSION     DAVMMR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.059"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(6..1220)
                     /locus_tag="VYS74_001674"
     CDS             complement(6..1220)
                     /locus_tag="VYS74_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3789678.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1670)
                     /locus_tag="VYS74_001675"
                     /pseudo
     CDS             complement(1376..1670)
                     /locus_tag="VYS74_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1832..2284
                     /locus_tag="VYS74_001676"
     CDS             1832..2284
                     /locus_tag="VYS74_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV3789679.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VYS74_001677"
     CDS             complement(2319..3707)
                     /locus_tag="VYS74_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3789680.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VYS74_001678"
     CDS             3773..4735
                     /locus_tag="VYS74_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV3789681.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4941..5951)
                     /gene="ccpA"
                     /locus_tag="VYS74_001679"
     CDS             complement(4941..5951)
                     /gene="ccpA"
                     /locus_tag="VYS74_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV3789682.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6376..6975)
                     /locus_tag="VYS74_001680"
     CDS             complement(6376..6975)
                     /locus_tag="VYS74_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3789683.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(6977..8074)
                     /locus_tag="VYS74_001681"
     CDS             complement(6977..8074)
                     /locus_tag="VYS74_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3789684.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8074..8811)
                     /locus_tag="VYS74_001682"
     CDS             complement(8074..8811)
                     /locus_tag="VYS74_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789685.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(8813..9697)
                     /locus_tag="VYS74_001683"
     CDS             complement(8813..9697)
                     /locus_tag="VYS74_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789686.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9684..9878)
                     /locus_tag="VYS74_001684"
     CDS             complement(9684..9878)
                     /locus_tag="VYS74_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789687.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(9875..10081)
                     /locus_tag="VYS74_001685"
     CDS             complement(9875..10081)
                     /locus_tag="VYS74_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789688.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3286 a         2415 c         1898 g         3063 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgctaaag gcgaggttaa gaacgtagag atagcaggta ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaaa actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ctaccaagac tgaaactagg
     1621 ataattccat aataaaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tcctgctatc ctttctttta ttttatcata tatttatata gagtttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgatcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaaaactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg taaataggtt gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta aggcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcatcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct atcccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg tgatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgaa ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg cccccttggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaattcca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcacgtc gccttaccgt atgtatggtt actgacttcg
     4801 tcagtttcat ctacaacctc aaaacagtgt gttgcgcagc ctttaaccag ccgcctagtc
     4861 cgctctatgg tattcattaa gtcaacatct cttgtttaag agcacaaaat caggaaatct
     4921 tctcgattcc ctgatttttt ctatttacgt tttcgtgttg agctacgttc tgtcaaacca
     4981 tgaggtaaga gaacttcacg ttcttccaac tcttccttat gcataatctt ggtcaacata
     5041 cgcatactaa tggcaccaag gtcataaaga ggttgggcaa tcgttgtcaa gtttggacgg
     5101 gtaaagcgtg agatttgtga atcatcacta gtaataattt caaaatcttc tggcacagaa
     5161 acacccttat cagccaaacc gttcaagact cctgctgcca actcatcacc tgtcacaact
     5221 gctgcagttg catttgatga aatcaaacgc tctgctaagg cgtaaccatc atcatagcta
     5281 tatttagatt caaataccaa accctcacta taagtgattc ctgctttttt caaggtttcc
     5341 ttgtagccaa ctaaacgaac cttaccattg atgtcatcca ctagcggacc gctaacgaaa
     5401 gcaatacgct cattttcttt agcaaggtaa ctcactgcat caattgttgc ttgcttatag
     5461 tcaatattga cacttggcaa ctggtgctca acatcgacag ttcctgcgag aacaatcgga
     5521 gtacgtgaac gcgaaaattc tgagcgaatt ttatctgtca agtgataccc catatagata
     5581 atgccatcta cctgctttga aaagagggta ttgacaacag aaacttcttt ctcgttatct
     5641 tcatcgctat tagctaggac aatattgtac ttgtacattt ctgcaatatc atcaatcccc
     5701 ttagccaaac tcgaaaaata accattggta atatttggaa tcacgacacc gacagtggtt
     5761 gtctttttac ttgcaagacc acgcgcaact gcatttggac gataatccaa acgatcaatt
     5821 acctctagca cttttttacg ggtattctct tttacatttt tattgccatt gaccacacgg
     5881 ctgaccgtcg ccatggaaac acctgcttca cgagcgacat cataaatggt tactgtatca
     5941 tctgcattca ttccttttcc tgtcctttct atctcacaca ttcttttaca agtagaggta
     6001 ctgattgaag ctctatatct acttacaaaa gtgaagatgt gaaaatttcg ttttcatatt
     6061 tctacttatt ccattctatc actaattgta aacactttca agtgtttttt gaagattgat
     6121 tgaaaaaatt tcatagaaaa cctaggttta gctccttgct accaccttag actaaacaaa
     6181 aaggaggaaa ctaagccctc ctaaagttat agtaaaatga aataagaaca ggataaatcg
     6241 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
     6301 ttcaatctac tataggtatt gttccattca ctaccgtcaa ttttagcaca tagtcttcat
     6361 gaaaatatta tatcatcata accaaccaga ttcttttgcg atattagctg cctctgttcg
     6421 attaccagca tccagttttg aaagaatatt ggtgacatag tttcgaatag ttccgtttga
     6481 gaggtaaagc tgatctgcga tttcttggtt ggataagccc cgggcgattc cctttaagac
     6541 agcaatctct tgttctgtca acggattggg acgggtcatc accatttcca tcaattcagg
     6601 cgaatactcc ttgcgtcctt cgaggacggt gtgcaaggtt tgcatgaggt ctgcaatgct
     6661 tcgttccttt aatacataag catccactcc agccttgacc gcacgttcaa aatacccagc
     6721 acgcttgaag gtcgtcacca caaccacctt tgtttcaagc ttttctgatc gtatccactc
     6781 caagacttca agacctgtct taacaggcat ttctacgtca aggatggcga tatctacaga
     6841 ctccttttct aatagttgga ttgcttcttg cccattcttg gcttgaagga cagactccac
     6901 atctggttga agcgtgagca attgacacat ggcatctcgc aacatacttt gatcttctgc
     6961 gactaatact ttcatcttag tttctctcct tataaggtag tcgaacttgc acttccgttg
     7021 gatgtttctg actgattaca cttacttctc ctgaaaatgg aaaaacacga tctcggactg
     7081 tatggagctc atctcctttt agaaaagcaa agccgcagcc atcatcactt actgttaaaa
     7141 tgagttcctt ctctgtccgt tctaatttta agtaagcttt agacgctttg gcatgtttga
     7201 tgatattggt caccaactca agcaaaatca tagaagccat tgactccaat tcttgagtaa
     7261 ggctagcagt atctagttgg ttatccgttt ccacctcaat tccagcaatt tctaacatct
     7321 ttttcacagt ctctagttcg gatgtcaaag ttctagactt aagattttcc acgatagttc
     7381 gaacttcatt catggaatcc ttgctgatct ggtgaatttc ttttaattcc ttttccacct
     7441 gtggataagc ctccatctga aataactgca aggctagatc agttttaaca ctaagcatag
     7501 caaaggtatg ccccagacta tcatgcaaat cttgaccgat acgattgcgt tcattttcag
     7561 caagcaatag atttatctgg gcattttgct tagcctgtgc ttctttcaaa tcctccacaa
     7621 ttcgaatccg aaccaagcca aaagtcatta aatcgacaaa agcaagaatt acaagtagat
     7681 agaatagaaa ctcaacttcg attctctgaa aaatcaacag ttgacccaca acaaggactt
     7741 gagcaagaag aaaagtccag acatgtaaag actttaaacc acctacgctg aaatgatagc
     7801 ttaagagatt ggataggaaa aagaaaaacc agatataatt aacagccaca aaggcagtat
     7861 tcccaactac ataagtcagc aggatgcccc aatatagcca agatagacgc tggctcttag
     7921 ttgttaaaac acccaaatac gccactacaa atagaatatc aatcaataaa tgccaggcag
     7981 aaagccaccc agtcactaca gttaggatgg ggaaaaccat aaaaattaaa ctgatccaaa
     8041 acatataatg tatgcttttc agtctttcaa gcattaagca ttctccttat gaccttgaag
     8101 gtaaatggtc aaaccaaaca aaactactga aaaaacaagt aaataaactg tggctgatag
     8161 attgatgcca ccctcattta agaaggtctt gagcaactcc atcaactgat aggtcgggag
     8221 acacttacct actacttgca tccagtctgg aaataaagag ataggcatcc agagtccacc
     8281 taaaacagcc aaccctagat aaagaagatt gcccacgaca gacatcaact gactagttgg
     8341 taagagagtc aaggtcaaac caagcgctac gaaggcaata cttcctacta tcagcaaaag
     8401 tgcagcccca atccaatttc caagagacat gtccacacct cttacaaaat gcccaactga
     8461 gaaaaccacc aagattgaaa ccaaataatc aaccagcata cttgttatct ttgatagata
     8521 atattctacc atatttacag ggctatgacg caatgttttc tgccagttgt tgatcttgtc
     8581 ggtatgtaaa acaactggga atgagaagat agctgttgac atcatggaaa atgcagtcat
     8641 ggagataaga taatcacgca taaaattcgc gagttcacct ggtgtgtcct gatagatacc
     8701 agaaaaaaat aaatagaaag ccgtcggcat ccctaccgac aatagataat agattaattg
     8761 tcgtttggtc aataaaaatt ctatcttact aagtgctatc catcgtttca tcttagttat
     8821 ctcccttctg cgtctcttca aagattgtat tcaacaaact acgattatta acttcgattt
     8881 cttgcatcct acatcctgct tgggctaata gttcccagaa agcatctgct tctcgagtga
     8941 ctacttgtaa agaatcttgc tttagggtcc agttttcaac caaatttgac tgctctacga
     9001 cttccttgta agctatagga aggataaagt gcttttctat ttcctcgcta cgcatagcta
     9061 gaggcgtcgt atctcgaatc aactctccct tatttaagag caaaatgcgg tctgcagtgt
     9121 gttccacttc ctcaatataa tgggacgaat agagaatggt gactccttgc gcttttaact
     9181 cctggacaat ttcccaaaaa cgttgacgag ttgaggtatc catggacgca gtaggctcat
     9241 ctaaaaaaac aatctttggt cgcccaatta aggtcaagat gaaagaaaag agacgctttt
     9301 gaccgcctga taatttttct gccaactgtt ctttttgttg cttgacaaac tgcaatagtt
     9361 gatcaatttc ctgattgctc aaaggatttg gatagatacg ttgaaagaaa gcaatcaact
     9421 ctttaacttt taatttctga acgattatat tttcttgagg gaggtaagcc ctagtatagt
     9481 ctaacttaga actcgtcact ggtaaacctt gaatagatac ttgaccgctt gtgaccagtt
     9541 tatctccaag caaacaatct aagagtgttg tctttccggc accatttgga ccaattaagg
     9601 caacacattc accatcgttg atttcaaagg agatgttccg caaaatttcc ttgtctttta
     9661 ttttcttact caatttttca actttaacca cagtcatgag actctccttt caaccactcc
     9721 attcccatag ggaaaacgac gaaaatcata aatccaaacc ccaaagcacc acaaatgaat
     9781 tggcgaagca aggcttgatc aaaccaatca gtaaacattt ccactaacca taccaagagt
     9841 gacaggccga taaagaaata gatgatccct ctcttcattc ctcaaactcc tttttcacat
     9901 ctctgactaa tttcaaacct tctctaacaa gccaagacat gattccaaag ccagcaaaga
     9961 ccttccaagg aaaatggtag aaatcctcat ccaatcccga aaacatgaga taggtcataa
    10021 ctcctgctac tactaaactc attgcaataa tcattttatt tctcatctct tcttccccca
    10081 tttcatacta caattatagt cttttaaaat cagaggaggc agaagcttct gtcactagaa
    10141 aatatgacaa atgtcataaa aattctgttc aaaacaagca agatacacta tacaataaaa
    10201 cacaatcaga aaaatctaag gcaacttcct caaaagagat atcaaaccca attcacacca
    10261 taatgtaaac taatacttat ttaaaatcaa aaagagtaga aatttttatc agacaaacac
    10321 atatatagtg tattgaatct ataacagtag gccttaaata ctaaaatatt tctataaatt
    10381 aatttaactt tcctgataga gctgttcata tcttatttca attctctata ttatacgttg
    10441 aacaaaaccc ttctattgct ttcttaaaaa tttataagag ttataaaatc tgttaaattt
    10501 caatgtgtat acctaaacta cggtatttat tgaaaagact ggagacaaaa agtatacgct
    10561 gccaaaatga attactgaaa atcaaaaaag agagaaccaa actgattccc tcttaatgta
    10621 tataatatct agttttaaaa atacacactc acatatctct gt
//
LOCUS       DAVMMR010000060        10617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMMR010000060 DAVMMR010000000
VERSION     DAVMMR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.060"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(69..299)
                     /locus_tag="VYS74_001686"
     CDS             complement(69..299)
                     /locus_tag="VYS74_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV3789689.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(467..1111)
                     /gene="pcp"
                     /locus_tag="VYS74_001687"
     CDS             complement(467..1111)
                     /gene="pcp"
                     /locus_tag="VYS74_001687"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3789690.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1126..2049)
                     /locus_tag="VYS74_001688"
     CDS             complement(1126..2049)
                     /locus_tag="VYS74_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV3789691.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2046..2735)
                     /locus_tag="VYS74_001689"
     CDS             complement(2046..2735)
                     /locus_tag="VYS74_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV3789692.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2800..3504)
                     /locus_tag="VYS74_001690"
     CDS             complement(2800..3504)
                     /locus_tag="VYS74_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3789693.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3560..4582)
                     /locus_tag="VYS74_001691"
     CDS             complement(3560..4582)
                     /locus_tag="VYS74_001691"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV3789694.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4714..7185)
                     /gene="parC"
                     /locus_tag="VYS74_001692"
     CDS             complement(4714..7185)
                     /gene="parC"
                     /locus_tag="VYS74_001692"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV3789695.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RTSENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7605..9548)
                     /gene="parE"
                     /locus_tag="VYS74_001693"
     CDS             complement(7605..9548)
                     /gene="parE"
                     /locus_tag="VYS74_001693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV3789696.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9684..10325
                     /gene="plsY"
                     /locus_tag="VYS74_001694"
     CDS             9684..10325
                     /gene="plsY"
                     /locus_tag="VYS74_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV3789697.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10368..>10617)
                     /locus_tag="VYS74_001695"
     CDS             complement(10368..>10617)
                     /locus_tag="VYS74_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789698.1"
                     /translation="FLESKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKL
                     LNQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3044 a         2392 c         1877 g         3304 t
ORIGIN      
        1 gcctcttcgc tatctctcct acaatcaaca tggactatta tatcatgaaa atttcaaaaa
       61 aacaagactt attttgctaa attataattt tcaatcaaaa tttccacagc tttttccaat
      121 tttttataaa aactatcgac attcccattc tttataatgg caaattgtcc gtctaattcg
      181 gcaatttctc cgataacttt tttaccgata gtcaatgtat aaccttcaaa actgtcttta
      241 ccaacattaa ctttggcatc cgctacttga atttcgattt ttttatcttt cttgctcatt
      301 tcatacctct cttcactttt tctattttac aagaaaatcc taaaactagc aagaaaaaac
      361 tctatatcag gcttctctga tatagagttt tatgtaaaca acctcaatcg atttagcata
      421 gctaatagtt agtaaacaag cataattgac tatccttttt aggttatcag tgagtctcac
      481 cgcctactaa ctgcaaatcc ttatctttgt agtctacaat agcttcaata gctacctcta
      541 tgcctcttac aatatcacat agactcattc ctgcagtatt cggcttattt actacttgtt
      601 ccatcatata tggaatatgc atgaaacctg ctctcatatt agggaatttt ttatccacta
      661 aataaagagc ttggtacatc aaatgattgc aaacaaaggt tcctgcacta ttggatacaa
      721 ctgccggaag tccctgtttt ttgatagctt gtaccatcgc tttgataggt aaactactaa
      781 aataggccga tgctccatca atacgaatcg gtgtatcaat tggttgattg ccttcgttat
      841 caggtatgcg agcatcatct tgattaatag ccactcgttc aggtgttaag ccggtcctgc
      901 cgcctgcttg tccaatacaa agtacagcat ctggttgata tcgtaatatt tctgcctcta
      961 aaacttctga cgacttataa aaaaccgttg gaatttctac ccagcgaact tcagccccat
     1021 taatctcaga tggtaataat tttacagcct ccaaagctgg attaatcttt tcacctccaa
     1081 aaggattaaa acctgtaacc aatattttca ttttattttc ctttactaaa atgcgagaaa
     1141 gtacattaag aatatgtgaa taacaatcat tactagagca acacctgctt gagcctttat
     1201 aacgccattc tgatctttca tatccatcaa tgctgctggt agagcgttaa aattagcagc
     1261 cattggggtc aataaggtcc cacaataacc tgctgtcatg gcaagagcac cagccacaat
     1321 tggattagct cccagagcaa atacaaaggg aactccaaca cctgctgtaa taacggtgaa
     1381 tgctgcaaaa gcatttccca taatcattgt gaatagaacc attccaagaa cataggccaa
     1441 aactcctata aagcgactat ctgaaggaac aataccgcta atcagatgag agataacatc
     1501 accaacacct gctacagtaa aaatagcccc caaagcccct aataattgag gaacaatacc
     1561 acttgttgaa acttgctgag tcattcgatt attttctgat aacagactct tagggtgact
     1621 attggtaatc acaagaacag aaattgtagc aaacaaggcg gcaaggctaa tcgaaatctt
     1681 gctaaattct ggaatcattt gcgctaagac caacgcaagt attgccatca gcataactgg
     1741 aataaaaatt ttatttttca acctgttaga ttcaatattg gctttcattt catctaagga
     1801 tggcaaggtt ccgatacgga cttgcttaaa caatgttaac agcgataata ggattacaat
     1861 aataccaata ctcatatttg gcatatagga accacctata aacgtaatag acaatagagt
     1921 ccaaaatgca gatgtcccaa gtcgaactgg gtttgtttta tctttataac tacaataggc
     1981 tgtatggaga aattgacaac caatcacaat ataggtcaac tctaatagtt gctttgccaa
     2041 ctctgtcatt tttgttctcc tcccctagtc ttttttgata tcaatttttt atcaaataaa
     2101 taattgtaaa tccccactac aataagtgtt ataacagcaa caataataga tgtagaagca
     2161 atccctgcat aattgctttc atagcctaac tgatctaatg ttccccctat caagaggact
     2221 cccccagcac ctacaaacgt attttgagca aagaaatttc caaaattttc attcgcagcc
     2281 gcacgcgctt ttattgtctc atcttcaacc tctgttaact ttctacctaa ttgagactct
     2341 gcagctgctt ctcccatagg ttgaaccaaa ggtctgacaa actgagggtg tcctcctaga
     2401 cgaattgaaa agaaaccagc taactctcga ataaaaaaat aaactgtata gaagtttcca
     2461 actgtcagac ctttaatctt tcgaatcaaa tcgattgatc gttgcttgag tccaaaggtt
     2521 tctgacagcc ccacaagagg caaggtaacc ataaaaatcg tgagcactcg ctgattgcta
     2581 aattcttttc ccaaaatctc caaaaattca acgagagaaa cacctgaaac taaagctgta
     2641 accaaaccag ctaagactac tgttgcaatt gtatcaaatt ttaaaataaa acccacaaca
     2701 atgattgcta ttcctattaa tctaatccac tccatatcaa actcctttat attcaaaatg
     2761 acagtatttt aaaaatttta tcaagatcaa taccattcct tatttaatgt atttttctag
     2821 ttctttttgg tatttgctat tggattccaa tttttctttt tgccattttt taaaaacctc
     2881 gttatattct tttgttgtaa caatatcttt ttgcaatttc attcctttaa agatatatgg
     2941 ttccccctta ataccaactt gtgagtatgg ttttgagaat ggtactacgt tacttacaac
     3001 tggagaacca ccagatgaag ctgttggcat caataatgaa ctatctgtcg accaagcttg
     3061 agctttggca tatttttcat atctcttctc taggtcagtg gtctcagaaa cagcatcttc
     3121 taacaatttc ttatatttat ccaaaccagg tttagctaca acatccttat cttttccttt
     3181 cgtaatacca aggtgtttca tggcagaacc agattttgga tccataatat tcaagtaaga
     3241 cgctggatct tgatagcttg gagcccatcc tgtactattc aaatcatagt ctttttgaga
     3301 aggagcaaca ttgccgtatt tatcattttc catcaaacca tcaataacat ttccaataac
     3361 gtctgtcctc gatgttcgag tcgctatact gtagcccaat gatgctggat ctactgcata
     3421 gacataagaa aatgttgtcg gtgcatctgc ttctttatca gtttttccac aagccactaa
     3481 aatagctgac gtgctcagga ccactcctgc tgttaagagc cactttttct atttcataaa
     3541 aaatctcctt tggtttattt taatctactt ttacaatcca accttctggc gcttcaatat
     3601 cgccaaactg aatacccgtc aattcattat ataatttacg cgtcacagga cctacttctg
     3661 tttcactata gaatacatgg aaatcatcac catgttgaat acctccaatt ggagaaataa
     3721 ccgctgctgt accacaggca cctgcctcta caaaacggtc aagattatca attggaacat
     3781 caccctcaat aggagttaat cccaagcgat gttctgccaa ataaagcaag gaatacttgg
     3841 taatagatgg caagatagat ggactcaatg gtgttacaaa ttcattatca gctgtaattc
     3901 caaagaagtt agctgatccg acttcttcaa tctttgtatg agttgatggg tccagataga
     3961 taacatctga gaaatgacgt gacttggcca tttttcctgg taagagactt gcagcatagt
     4021 ttccaccaac cttagccgca cctgtaccat ttggtgctgc acggtcgtac tcatcctgaa
     4081 tcaagaagtt ggttgggacc aaaccacctt taaagtaatt tccaactggc atagcaaaga
     4141 tggtgaaaat gtactcttct gccggtttta ccccgataat atctccgaca ccaatcaaaa
     4201 gagggcgaag atataaggtt ccacctgttc cgtatggtgg tacgtattct tcattcgcac
     4261 ggacaactgc tttacaagct tctacaaaca tgtctgtcgg aacttgtggc atcaagagac
     4321 ggtcacatgt acgttgcaga cgtttagcat tttcatcagg acggaacagt tgaacactgc
     4381 catccttagt acgataagct ttcaaacctt caaatgcttg ttgtccatag tgaagacttg
     4441 gagaagactc tgaaatatgc aaagttgcat cctctgtaag ctctccttga tcccattgtc
     4501 catttttgaa atgagcaaga tagcgataag gtaatttcat ataggaaaaa ccgaggtttt
     4561 cccaatcaat cgttactgtc atattccact ccttattcta aaaacctatt tcttatattc
     4621 tacactattt ttctaaaata gcaagtatat tttgtaattt tcagaaaatt tctccaataa
     4681 aaaccagctc ttagaactga ttcttcattt cacttattta tcttcagtaa ctacttcctg
     4741 aagataagcg tcaaaaactt cttcatctga aatcgtgtca gaaatgaagc ttccattgct
     4801 agtgcgttct gacaagttca agtcttgcaa tcggctttca tagattgttc ctttattgga
     4861 ttggacaagc agagtttggt cgttcacatc cacttccgta ctgaagaaat cgccaacaaa
     4921 tccttgctct gcaactgctc ctgccaagaa gacacgatgc ggtttgtttt tcaactcacg
     4981 caagacttgt aatcctcgtt tggcacggct ggttgctaga atttcctcaa tggaaacacg
     5041 tttcaagctt ccacgctggg tcaagaggta gaaggacgaa gtattacaga taaagccaga
     5101 ttggaggaca tcatcttctt tcaaattcat agccttgaca cctgctgcct tagcaccgac
     5161 aaccggaacc tcttcgatat tgaaacgcag ggcataacca ttttgactaa ccaagacaac
     5221 atcatctagt ttaatcggag ccactgctac aatctgatct gtatcgtctt tgagcttagc
     5281 atacttgaca gacttagatc tataggtccg ccatggagtg aattcttttc gctctacccg
     5341 tttgatttga ccaaggcgag tcactgcaaa gtaggttgtc gcatcgtcaa actgatccag
     5401 tacttccaca taaaggattt cttcattcgt ttcaaagttt gtgatggttt ggctcagatg
     5461 ctctccgatg tccttccaac gaatatctgc caactcatgg attggtctgt agatgacatt
     5521 tccaagactt gtgaacatca agaggtgctg ggttgtcttg gcagattgaa caaaaatcaa
     5581 acggtcatca tcacgcttgc caatttcttc caaggtggaa gccgcaaagg aacgtggact
     5641 ggtacgcttg atgtaacctg ccttggtcac gctgacgtag gtatcttcct cagcgataag
     5701 actagctgta tcaatctcaa ttgctttcgc agtgtcttct aaagaactca aacgaggagt
     5761 tgcaaatttc ttcttgacct cacgaagttc tttcttcatg agattgtaca tagtcctttc
     5821 atcaccgata atagccgcca gcatagcaat cttctcacga agctctgctt cttcttcctg
     5881 caagacaacc acatcggtat tggtcaaacg gtacagttgc aaagttacga tagcctcagc
     5941 ctgttcttcc gtaaagtcat agctaacttt gaggttttcc ttggcgtccg ccttattctc
     6001 agaagtacgg ataagagcaa tgacttcatc caaaatcgaa atcacacgaa tcaaaccttc
     6061 gacgatatgg agacgtttct cagccttttc tttgtcaaag cgtgaacgcg ccaaaatcac
     6121 ttctcgacgg tgagcgatat agctagacag gattggaaca atcccaacct gacgaggtgt
     6181 gaaattgtca atcgccacca tattaaagtt gtagttgatt tgtaggtcgg tgtacttaaa
     6241 taagtagttg agaacaagct cagtattagc gtctttctta agttcgatag cgatacgaag
     6301 accatcacgg tcagactcat cacgaacctc agcaatccca gctaccttgt tattaacacg
     6361 aacatcatcg attttcttga ctagattggc cttattgatt tcataaggaa tctcagtaat
     6421 aacgatttgt tccttaccac cttttagctt ttcaatttca gtcttggaac gaacaaccac
     6481 gcgccctttc ccagtctcat aagctttctt gatttcatca cgaccctgaa taatagcccc
     6541 tgtagggaag tctggtccag gcaagaattc catgagttta tcaatctttg cagttgggtg
     6601 gtcaatcatg taaactgcag catctatgac ctcagctaaa ttatggggag gaatgtctgt
     6661 ggcataacca gccgaaatcc cagtcgaacc attgaccaag aggtttggaa aggctgctgg
     6721 caagaccgtt ggttctttct ccgtatcgtc aaagttccat gcaaaaggaa ctgtcttttt
     6781 ctcgatatcc tgaagaaggt agcctgcaat ttcagacaaa cgtgcctcag tataacgcat
     6841 agccgcagga ggatctccgt ccatagaacc gttattaccg tgcatttcaa ctagaatctc
     6901 acgatttttc cagttctgtg acatacgaac catggcatca tagatagaag aatccccgtg
     6961 tgggtggaaa ttccccatga tgttcccgac tgacttggcc gacttacggt agctcttgtc
     7021 aaaagtattg ctatccttat tcatagaata aagaatacgg cgctgaaccg gcttcaaccc
     7081 atcacgaata tctggcaaag cccggtcttg aataatgtac ttggagtagc gaccaaagcg
     7141 ctctcccatg atgtcctcca gggacatatt ttgaatgtta gacataagat acaaagccca
     7201 taaaatacca agtgaaaata gaaaattctt gaagtaagca aactcacaag agaatttatc
     7261 tttttcacac agtatctagg gcgtgttcaa ctcctttcaa agaatgtaga gtaggttttt
     7321 atgcagtaaa agatatttta cgggaattcg tcccgtgttc agttacgata agtaaccaaa
     7381 ctatcctgtt tgtatttttc aatatgaaaa tctggttttc caaaattagt cttagtttgt
     7441 gtcttagccg ctcccttaaa cgcctctttg agataagcac tcatagaaga ttcttcatta
     7501 ataatcctgc aattttttca aaccaagatt ttcaaactgc ttttttacat agtcattcac
     7561 atccgactct aatttccagt ttactaacat attattttct ttcattaaaa cactgtcgct
     7621 tcttctagcg taaacttgac attatcttca atccatttac ggcgtggttc taccttatct
     7681 cccatgagaa cattgacgcg gcgttcggcg cgcgctaaat cttcaattgt gacacggatg
     7741 agggtacgtg tttctgggtt catggttgtt tcccagagct ggtccgcatt catctcacca
     7801 agtcctttgt atcgttggag ggtagcgcct ttaccgaact gtttacggag ttcttctagt
     7861 tctccgtccg tccaagcgta ggccacttct tctttcttgc ctttaccttt ggacatcttg
     7921 taaagaggtg ggagggcaat atagacatga cctgcctcga ctagcggacg catgtaacgg
     7981 tagaaaaatg tcaagagcaa ggtctggata tgggcaccgt cggtatccgc atcggtcatg
     8041 ataatgatct tatcatagtt ggcatcttca atagagaagt ctgctccaac acccgcacca
     8101 atggtataaa tcatggtatt gatctcttca tttttgagga tatccgccat cttggccttg
     8161 gctgtattga caaccttacc acgaagaggt agaatagcct ggaacttgcg gtcacgacct
     8221 tgtttggcag aaccaccggc agagtccccc tcaactagat agagttcatt cttagcagga
     8281 ttcttagatt gggctggggt caatttccca gacaacaagc ccttatcttt cttgtttttc
     8341 ttcccatttc ggctctcatc acgcgcctta cgtgctgctt cacgagcatc acgggccttg
     8401 atagccttgc ggatgaggtt agaagctaat tccccatttt ccataaggaa aaaggtcaac
     8461 ttatcagcca ctattccatc cacaactggg cgagctaggg ggcttcctag tttatccttg
     8521 gtctgtcctt caaactgcaa gtgttcttca ggaactaaga tagaaagaac ggccgctagt
     8581 ccctcacgat agtctgaacc ttcaaggttt ttatcttttt ccttgagaag acctgtttta
     8641 cgtgcatagt cattcatgac cttggtaatg gcagacttga gtcctgtctc gtgcgttcca
     8701 ccgtccttgg tgcgaacgtt attgacaaag gatagaatgt tatctgagaa tccgtcattg
     8761 tactggaggg ctacttccac ttgaaaacca ttgtcttccc cttcaaagta aagaactggc
     8821 gtcaagattt ccttatcttc gttgagataa gaaacaaaat cttgtactcc attctcatag
     8881 tggaactcaa tcgcttcatc tgttcgcttg tccgttaaag acaaggtcac atttttcaag
     8941 agaaaggctg attcattaag gcgctctgaa atggtattgt acttgaaatc tgtcgtagaa
     9001 aagatagtcg cgtcaggcat aaaagtaact ttggtgcctg ttttagactt gggtgctgta
     9061 ccgattttct tcaaagtcgt gacaggtttt ccaccatttt cgaaacgttg cttgtaaact
     9121 gcgccatcac gggtaatttc aacttctaac cagctagaaa gggcgttaac aacggaagaa
     9181 cccactccgt gaagtccacc tgatgtctta tagccacctt gaccgaattt ccctccggca
     9241 tgaagaatgg taaagataac ctcaacagtt ggaattccca tagcgtgcat acctgtcggc
     9301 atcccacgtc catggtcttg aaccgttaga ctaccgtctt tattgatagt tacatcgata
     9361 cgatcaccaa acccagacaa ggcttcatcg actgcattat caacgatttc ccaaactagg
     9421 tgatgaagac cagcgccatc ggtcgatcca atatacatcc ccggacgttt tcggaccgca
     9481 tccaaccctt ctagcacctg aatagcatca tcattataat tgttaatatt gatttccttt
     9541 tttgacacaa ggaacctcct attcgttcat ctttactatt ctacaggttt tccaaggatt
     9601 ttgcaaaatt tttctttctc cgatgtgaca atttcagcag agattctctg cttttctttc
     9661 ccaattcatg atataatagg agtatgatta caatagtttt attaatccta gcctatctgc
     9721 tgggttcgat tccatctggt ctctggattg gacaagtatt ctttcaaatc aatctacgcg
     9781 agcatggttc tggtaacact ggaacgacca ataccttccg cattttaggt aagaaagctg
     9841 gtatggcaac ctttgtgatt gactttttca aaggaaccct agcaacgctg cttccgatta
     9901 tttttcatct acaaggcgtt tctcctctca tctttggact tttggctgtt atcggccata
     9961 ccttccctat ctttgcagga tttaaaggtg gtaaggctgt cgcaaccagt gctggagtga
    10021 ttttcggatt tgcgcctatc ttctgtctct accttgcgat tatcttcttt ggagctctct
    10081 atcttggcag tatgatttca ctgtctagtg tcacagcatc gatcgcggct gttatcgggg
    10141 ttctgctctt tccacttttt ggttttatcc tgagtaacta tgactctctc ttcatcgcta
    10201 ttatcttagc acttgctagt ttgattatca ttcgtcataa ggacaatata gctcgtatca
    10261 aaaataaaac tgaaaatttg gtcccttggg gattgaacct aacccatcaa gatcctaaaa
    10321 aataaaatgc cagttctgta ctgcccccaa acagttagac aaataattta tccaaaggat
    10381 ttagttctgt actgcacagg actaagtcct tttagtttta ccttaattcg tttgttgttg
    10441 tagtaatcaa tatagtctat aatggcttgt tccaattggt taagtaattt aaatgttttc
    10501 tcatagccat aaaacatttc ggattttaaa atgccaaaga aagattccat cataccgttg
    10561 tcttggctgt ttcccttgcg tgacatagat gcttgaattc ccttactctc taggaac
//
LOCUS       DAVMMR010000061        10372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMMR010000061 DAVMMR010000000
VERSION     DAVMMR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.061"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            1..201
                     /locus_tag="VYS74_001696"
                     /pseudo
     CDS             1..201
                     /locus_tag="VYS74_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            299..511
                     /locus_tag="VYS74_001697"
                     /pseudo
     CDS             299..511
                     /locus_tag="VYS74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            411..857
                     /locus_tag="VYS74_001698"
     CDS             411..857
                     /locus_tag="VYS74_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3789699.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(995..2299)
                     /gene="eno"
                     /locus_tag="VYS74_001699"
     CDS             complement(995..2299)
                     /gene="eno"
                     /locus_tag="VYS74_001699"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV3789700.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2463..2909
                     /locus_tag="VYS74_001700"
     CDS             2463..2909
                     /locus_tag="VYS74_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV3789701.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2906..3709)
                     /locus_tag="VYS74_001701"
     CDS             complement(2906..3709)
                     /locus_tag="VYS74_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV3789702.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3718..4047)
                     /locus_tag="VYS74_001702"
     CDS             complement(3718..4047)
                     /locus_tag="VYS74_001702"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV3789703.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3992..4363)
                     /locus_tag="VYS74_001703"
     CDS             complement(3992..4363)
                     /locus_tag="VYS74_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV3789704.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4422..5855)
                     /gene="glgA"
                     /locus_tag="VYS74_001704"
     CDS             complement(4422..5855)
                     /gene="glgA"
                     /locus_tag="VYS74_001704"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV3789705.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5852..6991)
                     /gene="glgD"
                     /locus_tag="VYS74_001705"
     CDS             complement(5852..6991)
                     /gene="glgD"
                     /locus_tag="VYS74_001705"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV3789706.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6981..8123)
                     /locus_tag="VYS74_001706"
     CDS             complement(6981..8123)
                     /locus_tag="VYS74_001706"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV3789707.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8113..10041)
                     /gene="glgB"
                     /locus_tag="VYS74_001707"
     CDS             complement(8113..10041)
                     /gene="glgB"
                     /locus_tag="VYS74_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV3789708.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3179 a         2265 c         1819 g         3109 t
ORIGIN      
        1 tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa tagtttaaag
       61 cacctagcac aggtctaaca aatgaatggt gagttaatcg ctccaatgac ttacgaagag
      121 acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc aacattaacc
      181 gcaccattgg ttattatatt aaaacgaagt aagaatagga caaatcgatc aggacagtaa
      241 aatctaattc taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctactat
      301 attaatggac aatgcaagat tccatagaat ggggaagcta gaactcttgt gtgaagagtt
      361 tgggcataaa cttttacctc ttcctcccta ctcgcctgag tacaatctta ttgagaaaac
      421 atgggctcat atcaaaaagc acctcaaaag ggtattacca agttgcaata ccttttatga
      481 ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc gaaacaaccg
      541 ataagaattg gcacaaaagc gaccgtattt ttgttaccaa tacaggaaaa ccagttcata
      601 gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa ccaattccca
      661 aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca gaaaataaaa
      721 ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc actacttcca
      781 tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg gataaagtta
      841 tgaaaaagat tttttaaaaa gttttgtccc ttttttgtcc tctaaataca aaaatagccc
      901 ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac ttttgaattt
      961 catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg tagaatgatt
     1021 tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc aattggttgt
     1081 atttagcgat gcggtctgta cgtgaaagtg aaccagtctt gatttgtcct gcgttagttg
     1141 caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt gatacaacag
     1201 cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta agagtaccga
     1261 tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca cgtgcaaggt
     1321 agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta ccaagacgtt
     1381 cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca atagtgatga
     1441 ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat gtacgaacag
     1501 cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta tcgtagaatt
     1561 ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat ccagcagctt
     1621 caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga ggagcgaatc
     1681 caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc ttaagagcgt
     1741 ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca actggcaaga
     1801 tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg atgatgttca
     1861 tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa agtgggattt
     1921 caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt gcattcgcac
     1981 ccaatttacc tttgttagga gtaccgtcaa gtgcaatcat agcacggtca atagcttgtt
     2041 gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg ttgtcaacag
     2101 ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt tcaactgctt
     2161 cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca cctgattcag
     2221 tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg cgagcgtaaa
     2281 catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta cacctctata
     2341 ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac ttttccttaa
     2401 ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga tatactgttt
     2461 tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta aaaataaatc
     2521 cggatgagca aagacgctat cttggtactt ttgaggaaag agttcttgga tatgtagata
     2581 ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta gaaaaccttc
     2641 aggaaaaagc aaagccacta tttgtgaaga tttcaccaac tatcgaattt gataagcaag
     2701 ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta tctgaagagc
     2761 atattacttc tccttttggc ctggttattc atagcaatgc accagttcaa gtagaagaaa
     2821 aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa ccagccaaaa
     2881 catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag tgcccaatat
     2941 tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct aaaggttcac
     3001 ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga ggtaaatctt
     3061 cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt tttgcaacac
     3121 ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca acaacaacca
     3181 agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta tcaattccag
     3241 acacgatact tgcctgagca aaggcacaca aaccagcagc aatccccagt cctccatcat
     3301 tactggccgt gccaccaaca ccgatataaa tatctttaat ccctttagaa atgagatgga
     3361 gaatcaactc tccaatacca caagtttgga tttgaagtgg atttcgtttc tctagcggaa
     3421 tttttccaag accaaccaag tcagctactt caaatagtgc cagttcccct ttttgaaaat
     3481 agcgcatggc ttctttttgt ccaaaagggt ctgtcacttg gatccatttt tcttttaggt
     3541 caagagaatg tcggatagca tctacagtac cttctccccc atcaccaaca gggcagagga
     3601 gacattctac atctgctatc gattgttgga agcctctttt tattgcttca gctacctgtt
     3661 gagctgtcaa gctttcctta aacgaatccg gtgcaattac aatcttcata ttttccctca
     3721 ttctaaacag tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa catgatgtgg
     3781 tatttctttt ttgagcattt ctttggaaca aaaggcgatt cctaactccg ccgacttcaa
     3841 cattaataga ttattaaccc catcaccgat tgccaccgtt ctttctttag aaagttttag
     3901 tttctttctc catttttcca gagtttcttt tttgacctgg ggacttataa tttgtccaac
     3961 taattttcct gttaaaagac cttctttgac ttcaagctgg ttggcagtga aataggcaat
     4021 accaagggat tttgctaatc tctccaacta ttggtgtaaa tccaccagac accagaccaa
     4081 ctaggatgcc attcttttgg agaatagaga tgaactctgg gacatttagc gatagatgaa
     4141 ttgagttgaa gacgttatca aagaccaaaa taggaagacc ttccaacaag gacactcttt
     4201 ttcttaaact gctttcaaag accaactctc ctcgcattgc tcgacttgta atctgcgaaa
     4261 tttccgcctc atgacctgcc tctctcccta aaagatcaat cacttcttct aggattaagg
     4321 ttccatctac atccaaaaca cacaagcctt ttacttgaga catcagttct cctctctaaa
     4381 cagcctaaaa atcgtatgaa gtcatcatac gattttatct attaattaac taaactatgg
     4441 tacaagtcaa ggtatgactt gcaggctgta tcccatgaga agtcactctc catagcttgt
     4501 ttttgtaggt ttctccaaat gtctggatga tttctataca agtccaatgc tgtttggaaa
     4561 gtccaattta accaataagg agatagattg tcaaagctaa agccagtacc gcttccttcg
     4621 attggattga aagcgcgaac tgtatctcgc aagcctccaa cttcatggac caatggcaag
     4681 gttccataac gcatagccat catttgagac aagccacacg gttcaaaacg acttggcatg
     4741 aggaagaggt cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa agtgatattt
     4801 gctgatagct tgtctgggta aatctgagca aaccatgaga aagctccttc aaaggctgga
     4861 tcgccagttc ccaaaagaac aatctgaaca tcttcttgca agatatggtg aagactttcg
     4921 accaccacat caaaaccttt ttgacgtgtc aaacgagaaa caattcccac cagtggaacg
     4981 tctgctctaa caggcaagcc aactctttct tgcaattttg ccttattttt ggctttccca
     5041 gacaaatctt cctgattgaa atgatagtct aaaagagcat ccgtctgagg attataaaga
     5101 tcagcatcaa tcccattcac gataccagat actttaccag actccatttt aagaatctga
     5161 tccaaattac atccaaactg actagtcata atttcatgag catagctagg tgaaacggtt
     5221 gaaacacggt tcgcatagag aatacctgcc ttcatccagt tcagacagtt gttccatcga
     5281 agggtgccat cagcgtaacg ttcaaggcca actccaaaca aatcacccaa cattccttct
     5341 gaaaattgtc cttggaattc taaattatga atggttaaaa ctgtttcaat gtcctcatag
     5401 gcttgaatcc aacagtattt ttccttcaac aagaaaggaa tcatagctgt atggtagtca
     5461 tgaacatgga gaagatcagg aataaagtca atcctttcca tagcctcaat ggcagccagt
     5521 tggaaaaagg caaagcgttc tccgtcatca aaatcaccgt aaacatgacc acggaagaaa
     5581 taatattgat tgtcaataaa gtagaaggtt acaccattta atactgtttt cttaattcca
     5641 caatactgtc tacgccaacc aacgctcacc tcaaaatgaa gcacatcttc aatctgattt
     5701 ccaaatttag cctctaccat atcatagtag ggtaaaatca ctgcaacttc gtgcccagct
     5761 tttaccagtg attttggaag agcgccaatg acgtctccca aaccacctgt ttttgaaaag
     5821 ggtgcaccct ctgctgctac aaataaaatt ttcatgaatg aatatcctct gttactttag
     5881 cacctttctt aaccacaact ggatgttctg cagttcctcg aatcacaaca ccaagctcaa
     5941 cttcaacccc tttgtccaag atagcatatt cgacctgagc cccttctcca ataacaacac
     6001 gagggaagag caggctatct ttaaccaagc tatccttatg gacatgaata ttacgtgata
     6061 gaacagaatt agctacttga ccttcaataa tactaccaga ggcaaactga gaagtgctta
     6121 ccttagatgt attagcataa taagttggct cttcgttttt gacctttgta taaatctttt
     6181 ggtttggtga gaaaagagaa tagaattttt gtgattcaag catatcgata tttgcttgat
     6241 aataagattt aacagagtga atattggcta gatagcccgt gtactcgtag gcaaaagctc
     6301 cctcttttac agccaaatcc cgtaaaacat agcgcaattt ctctggatgt tcttttttag
     6361 cttcttcttc caagtgttca atcaaccaag gtgtatcaac gacaaagata tctgtagaca
     6421 tattgaacgt ttcagctgtt gacttgctat caaagagttt atgagaaaga acatggtctg
     6481 tttcatctac atccaagatt gcatttactt ctgaaatatc tttcttagct agttttttat
     6541 aaactacagt gataggctct tttgttgtac tatgtaggtg gaaaacttgg ttcaaatcaa
     6601 tgttaataag aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa cgtttcaaat
     6661 aagtaagaag ctgttggtag tattcttttc caactgtact actttctaca cgggtattgt
     6721 aaattcctag atagtaatgg ctaagaaggg ttgataagcc ccactcgcgc cctgaacgaa
     6781 tatggtcaaa gactgagctg atattatcct gctggaaaat accaaagaca ctacgaacac
     6841 ctgcattagc aaggcttgaa agtgggaagt caatcaaacg atatttccca ccaaatggca
     6901 aacttgctac tggacggtgg tccgtcaatg tcgacatatc gtgaaaacca actgtatttc
     6961 ctaaaatggc agaatattta tcaatcttca tctgttgcta cccccactac ttcattatat
     7021 cctacaactt gtacttcatc tgttccatca atttcgacac cgtcagaaat aatcgcacct
     7081 tcaccaataa tggcacgttt aatcttagct ccttgaccaa taatagctcc actcatgata
     7141 actgaatcaa ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag gatagaatgt
     7201 ttaatagttc catcaacgaa acatccgtct acaactaatg agtcttccac atgagcattt
     7261 gccccgagga agtttggtgg tgaaatcaag tttcttgagt aaatcttcca ttgacggtta
     7321 cgactatcca aggcattttc tggagaaata tactccatgt tcgcttccca aagtgactca
     7381 atagtaccaa catctttcca ataaccacta aattcgtaag cataaacact ttcacctgac
     7441 tcaaggtaat ttggaatgac atttttacca aagtctgaca tgccaacctt gctcttttca
     7501 gcagcgacta acatattacg aaggcgttgc caatcaaaaa tgtagattcc catagaagct
     7561 tttgtagatt taggttgagc tggtttttct tcaaattcaa caatacgatt gttagcatct
     7621 gtgttcatga taccaaaacg gctcgcttct ttaagaggga cgtctaaaac tgctactgtc
     7681 aagctggcat tattatcctt atgagactgg agcatatcat catagtccat tttgtagatg
     7741 tggtccccag acaaaatcaa gacatactca ggattgacac tgtcgatata gtcgatattt
     7801 tggtaaatag cgtgactagt tccctcaaac caacgatttc cttcacttgc agaataaggt
     7861 tgaagaatag agacacctga attaataccg tctagtcccc agcttgaacc attcccaata
     7921 tggttgttga gagcaagtgg ttgatactgt gtaaccaccc caacattatg aatccctgag
     7981 ttggcacagt ttgatagggc aaagtcaatg atacggtagc gcccaccaaa ttgcacagct
     8041 ggttttgcga tgctttgagt gagtttaccg agacgagttc cttgcccacc agcaagaatc
     8101 aaagctaaca tttcattttt cattttctac tcctttttgg tttttatttg tgacggtttt
     8161 agtagatttc aagcgacgtt tgattttcca tacacttgct cccatagccg gtagggtaaa
     8221 ggttaaggtc tgctcataat ctttccatag gccttcttgc gtttgaacag tttgattatg
     8281 ttctttccaa acgcctcccc actcttccaa ctcagtattc catacttctt cgtaaattcc
     8341 tgcaacgggt agtccgattg taaaatcttt ccgctcaaca ggtaccatgt taaagataca
     8401 gactaacatt tctccctttt tacccttacg aataaaggaa agaacactct ggtctcgatt
     8461 atccgcatca atgatttcaa taccatcata gctggtatcg atttcccaca gacagcgatg
     8521 atctttgtaa aactggttta gctgagaagt gaaatacttc atcttagcat tcattgggtc
     8581 ttctaggtta gaccattcca actgttcttc agatttccat tctaggaatt gaccgtattc
     8641 gctacccatg aagagcaatt tcttaccagg gtgacaaatt tggtacgtat agagattgcg
     8701 caagcctgcg aattgattgt aacgatctcc ccacatctta tgcatcatac tcttcttgcc
     8761 atgaaccact tcatcgtgcg agaatggcaa gagataattc tccttgaaaa catacataaa
     8821 gctgaaagtc accaggttaa agtcatattt acgataaatc ggatcttctt cgtagaaacg
     8881 gaggatatca ttcatccaac ccatgttcca tttgtagtca aatcctagac caccaatctc
     8941 tttcattccc gtaatcttgg tcgcagacga actttcttct gcaatcatca tcacatctgg
     9001 atattctaac ttaataactt cattcaagcg ttgaagaaaa taataacctt catagttgag
     9061 atttccgcca tctttattag gtgtccatgg agcattatca tagtccaaat agagcatgtt
     9121 gctaacagca tccacacgaa taccatccaa atgatagaca tcaatccaat gcttaatgca
     9181 agaaattaag aaggactgga cttcattttt tccaaggtca aaattaaggg caccccaacc
     9241 atggttatga gccttattat ggtcttggta ttcaaaagtc ggtgtcccat cataataggc
     9301 taaggcatca tcgttgatgg taaagtgacc tggtacccag tccacaataa ccccaatatt
     9361 atgggtatga cactcctcga caaaatcttg aaactcctct ggtctgccat aagcatgctc
     9421 taaagcgaag taacccataa gctgataccc ccaactcaag cccaaaggat gggacatcaa
     9481 gggcataaac tcaatatgag tatagttcat ttcaacgaga taaggaatga gttcatcctt
     9541 gagctgggca aaactataag gactgccatc agaatttctt ttccatgatc cagcgtgaac
     9601 ttcataaata ttgacaggac gctcttcaaa gccccaacgt tttcttcgtg ccagccaaag
     9661 tccatccttc catttcttct caggaagctc tgttaagatt gcccctgttc ctggacgagc
     9721 ctcatacctg acagcaaaag ggtcaatctt catcagttga tgaccatttt gacgtgtgac
     9781 atgatatttg taaatatgcc cttcttgagc catattggta aagacttccc agaccccaaa
     9841 atcatttctt accattggaa tctgattttc aatccagttg gtaaaatcac caaccaagtg
     9901 aacagcctga gcattaggtg cccaaacacg gaaggtatag ccatgctctc catttagttc
     9961 ttccctatgt gctcctagat aatgttggag ataaaaattt tcacccgtca taaaggtttt
    10021 taatgcttct ctattatcca tatactcccc ttctcctgta agcgttttct atgtttttat
    10081 tatactacct ttttagagaa gattcaagta aattactata cttctttaat tattttgaaa
    10141 atctacaaca agttcactta ctcgttcaat tgtaaatcaa tattttttca aaaaattgcg
    10201 aaaacgcctt tctttttcta ctatagtgaa atgaaataaa acatgcgcaa gtcgattaag
    10261 gaatttaatc taatttctaa caatgtctta gaaatcaaag tgtactattt taacttcaat
    10321 gtactatata tcgcaaaatg aaataataac aggacaaatc gatcaggaca gt
//
LOCUS       DAVMMR010000062         9852 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVMMR010000062 DAVMMR010000000
VERSION     DAVMMR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.062"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            80..544
                     /locus_tag="VYS74_001708"
     CDS             80..544
                     /locus_tag="VYS74_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV3789709.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            701..1126
                     /gene="lacA"
                     /locus_tag="VYS74_001709"
     CDS             701..1126
                     /gene="lacA"
                     /locus_tag="VYS74_001709"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV3789710.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1157..1672
                     /gene="lacB"
                     /locus_tag="VYS74_001710"
     CDS             1157..1672
                     /gene="lacB"
                     /locus_tag="VYS74_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV3789711.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1683..2612
                     /locus_tag="VYS74_001711"
     CDS             1683..2612
                     /locus_tag="VYS74_001711"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV3789712.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2614..3594
                     /gene="lacD"
                     /locus_tag="VYS74_001712"
     CDS             2614..3594
                     /gene="lacD"
                     /locus_tag="VYS74_001712"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV3789713.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3828..4664
                     /locus_tag="VYS74_001713"
     CDS             3828..4664
                     /locus_tag="VYS74_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3789714.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4700..5017
                     /locus_tag="VYS74_001714"
     CDS             4700..5017
                     /locus_tag="VYS74_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3789715.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5019..6713
                     /locus_tag="VYS74_001715"
     CDS             5019..6713
                     /locus_tag="VYS74_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3789716.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6922..8328
                     /gene="lacG"
                     /locus_tag="VYS74_001716"
     CDS             6922..8328
                     /gene="lacG"
                     /locus_tag="VYS74_001716"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV3789717.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8418..8630
                     /locus_tag="VYS74_001717"
     CDS             8418..8630
                     /locus_tag="VYS74_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV3789718.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(8909..9670)
                     /locus_tag="VYS74_001718"
     CDS             complement(8909..9670)
                     /locus_tag="VYS74_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3789719.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3158 a         1663 c         1981 g         3050 t
ORIGIN      
        1 tggcattatc aatcatgaac taggattcta gttttgcagg aagtctaata aagaaactag
       61 caaggtcagg ccttacttta ttggtggaca tccaggattt aattgtcctc tacttgatga
      121 tgaagtctat gaagattatt atctagagtt tgagaaagaa gagacttgct ctgttccacg
      181 tcctttccca gaaacaggta tgttggattt ccaagataga agtccatggc tagagggaca
      241 aaaagaaata gatcttagtt atgatctttt cagcaaagat gcagtgactt tggatgaatt
      301 gcaatctcga acaattgccc ttcgttctct taaacatgat aggggattga aagtgcattt
      361 tgcagaattt ccaaacctca tcatctggtc aactttgaac aagggacctt tcattacctt
      421 tgaaccatgg tctggcttgt caacattcct tgaagaagga gatcatttag aagataagaa
      481 gaatgtttgt ctcttagaag ccaatcaggt tgaagaatta gggtttgaga tagaagtttt
      541 ataaaaaagt aaaaaataaa taacaaaaac aaacacaaac tgttgacttc ttcaaacaat
      601 agtagtatat tatgtttgtg aggactaatt gactgttttt taaagacagc caatacactg
      661 ctggaatcca gcatagaaaa aataaaaagg agtatacaat atgtctattg ttatcggtgc
      721 agatgctgca ggtttgagat tgaaagaagt tgtgaaagac ttcttagaaa aagaaaactt
      781 ccaccttgtg gatgttacag ctgaaggtca agactttgtt gatgtgactc tcgctgttgc
      841 tgcagaagta aacaaagaag aacaaaacct tggtatcgtg attgatgctt atggagctgg
      901 tccatttatg gttgcaacta agatcaaagg aatggttgct gcagaagttt ctgatgaacg
      961 ttcagcttat atgactcgtg gccacaacaa ctcacgtatg atcactatgg gtgcacaact
     1021 tgttggtgat gaattggcta aaaacatcgc taaaggattt gttaatggta aatacgacgg
     1081 tggtcgtcac caaatccggg ttgacatgtt gaacaaaatg ggctaattag attatagtaa
     1141 gaaaggtaag ttaaaaatga gaattgcaat tggatgtgac cacatcgtaa cagatgaaaa
     1201 aatggcggtt tcagaatttt tgaaatcaaa aggatatgaa gtcattgact ttggtacata
     1261 tgaccataca cgtactcact acccaatctt tggtaaaaaa gtaggggaag ctgtaactag
     1321 cggtcaagct gatcttggag tatgtatctg tggtactggt gttggtatca acaacgctgt
     1381 aaataaagtt ccaggtgttc gttctgcctt ggttcgtgat atgacaacag ccctttatgc
     1441 taaagaacaa ttgaacgcta acgttattgg ttttggtggt aaaattactg gtgaattgct
     1501 tatgtgtgat atcatcgaag ctttcatcca tgctgaatac aaaccaactg aagaaaacaa
     1561 aaaattgatt gcgaaaattg aacatgttga aagtcacaat gctcaacaaa cagacgcaaa
     1621 cttcttcact gaattccttg aaaaatggga tcgcggagaa taccacgact aagaggtaac
     1681 ctatgatttt aacagttaca atgaacccat ccattgatat ttcctatccc ttggatgagt
     1741 tgaagattga tactgtcaat cgtgtggtgg atgtaaccaa aacggctggt ggtaagggac
     1801 tcaatgttac ccgagtactt tcagaatttg gcgattctgt tcttgctact ggtttagtgg
     1861 gtggcaaact tggtgagttt ttggttgaac atatcgataa tcaagtaaag aaagatttct
     1921 tctcaattca gggagaaact cgtaactgta tcgctattct ccacggagac aaccaaacag
     1981 aagttcttga aaaaggtcct gaagtattgg aacaggaagg tcaagacttt ttggaacatt
     2041 tcaaaaaact cttggagtca gttgaagtag tggccatctc aggtagtctg ccagctggcc
     2101 ttccagttga ttactatgcg agcttggtag aacttgctaa tcaagctggc aagcctgtag
     2161 tcttggactg ctcaggtgca gcacttcagg ctgttcttga atcaccccat aaaccaacag
     2221 tcatcaaacc aaataatgaa gaattgtctc agcttcttgg aagagaagtt tctgaggatt
     2281 tggatgaatt aaaagaagta cttcaagaac ctttgtttgc agggattgaa tggattatcg
     2341 tttcacttgg tgccaacggt acttttgcca aacatggtga cactttctac aaggtagata
     2401 ttcctagaat tcaggtggta aatcctgttg gatctggaga ctctactgtg gcaggaattt
     2461 cttcaggact tcttcacaaa gaatcggatg cagaattact catcaaggca aatgtccttg
     2521 gtatgctcaa tgctcaagaa aaaatgactg gtcatgtcaa catggccaac tatcaagttc
     2581 tatatgatca attaatagta aaagaggtat aaaatggctt taacagaaca aaaacgtgta
     2641 cgcttagaaa aactttctga tgaaaatggt atcatctcag ctcttgcatt tgaccaacgt
     2701 ggtgctttga aacgcctcat ggctcaacac caaacagaag aaccaactgt ggcccaaatg
     2761 gaagaactta aagtcttggt agcagatgaa ttgactaaat acgcttcatc tatgcttctt
     2821 gaccctgagt atggacttcc agcaactaaa gctcttgatg aaaaagctgg tcttctcctt
     2881 gcttatgaaa aaacaggtta tgacacaaca agcacaaaac gcttgccaga ctgcttggat
     2941 gtctggtctg caaaacgtat taaagaagaa ggtgcagatg cagttaaatt cttgctttac
     3001 tatgatgtag atagctcaga cgaactcaat caagaaaaac aagcctacat cgaacgcatc
     3061 ggttctgagt gtgtggctga agatatccca ttcttccttg aaattcttgc ttacgatgaa
     3121 aaaattgcgg atgcaggttc tgtagaatac gcgaaagtaa aaccacacaa agttatcggc
     3181 gctatgaaag tcttttcaga cccacgcttt aacattgatg tcttgaaagt tgaagttcct
     3241 gttaacatta aatatgttga aggcttcgct gaaggtgaag tagtttatac acgtgaagaa
     3301 gcagcagcct tcttcaaagc gcaagatgaa gcaacgaact tgccatacat ctacttgagt
     3361 gctggtgtat cagctaaact cttccaagat actcttgtat ttgctcatga atcaggtgca
     3421 aactttaacg gagttctttg tggccgtgca acatgggctg gatcagttga agcatacatc
     3481 aaagatggtg aaacagcagc tcgcgaatgg cttcgcacaa ctggatttga aaacattgat
     3541 gagctcaaca aagttcttca aacaacagca acttcatgga aagaacgtgt gtaagaaagt
     3601 cctcctagtt taggaacatg aatctaaaaa aatttaaaaa aagttgtatg taaaggttta
     3661 caaaataact tacttgtgct atacttaaat cacaagttaa tacaaggtga gtgttactaa
     3721 gtaatattag gcatgatcac aggtgaatta gaaatcagct gattttctag ttcatttgtg
     3781 gtcatttttt gtactcatat acctttaaga tataaaagga ggttgacatg tatcgaattc
     3841 taaatccaat gaatcacaat gtctcgcttg tcagaaatga taagggagaa gaggtgattg
     3901 taattggtaa ggggattgca ttcggaaaga agaaggggga tttgattgct gaaaatcatg
     3961 ttgagaaaat ctttcggatg aagaccgaag agtccagaga aaactttatg gctcttctca
     4021 aagatgttcc gcttgatttt atcacagtga cctatgaaat cattgataag ctatcaaaga
     4081 aatatcatta tccgattcaa gagtatctct atgtaacctt gacagatcat atttactgtt
     4141 cttatcaagc tctaactcaa ggaaggtaca aggatagtaa tctgccagat atttctgcta
     4201 agtatcctgt cgcttttcaa atcgcaaatg aagcttttga aatttactgt cagaagctag
     4261 cagatcattt tcctgaggac gaaattattc ggattgctta tcatttcatt aatgctgaag
     4321 gtgaaaatga agtggaactt gtggagtcga ttgataagag gaaagaaatt ctcaggaatg
     4381 ttgaagaagt tttaacggac tatgcaattc aacgaactaa aaagaataac catttctatg
     4441 atcgctttat gatccatttg aattatttct tggattattt agacagatct agagatgata
     4501 accaatcact tctggatatg gaagatcata ttaaacaatc ctatccaaaa gccttcgaga
     4561 ttggttccaa gatctatgat gtgattacgc aacatacggg tcttgatttg tataaaagtg
     4621 aacgagttta tctagttcta catatccaac gtttattgtc ataaaaattt atttaaaact
     4681 atataaggag aattctatca tgaatagaga agaagtaaca ttgttaggtt ttgaaatcgt
     4741 agcctatgct ggcgatgctc gttcaaaact attggaagcc ttgaaggctg ctgaagctgg
     4801 tgattttgaa aaagcggacg ctctggtaga ggaagctggt agctgtattg cagaggctca
     4861 ccacgcgcaa acaagtctat tgactaagga agcttctggt gaggacttag cgtatagtgt
     4921 gaccatgatg catggtcaag atcacttgat gacaactatc ttgttaaaag acctaatgca
     4981 ccatttaatt gaactttaca agagaggagt taaataaaat ggataagtta ataattttta
     5041 ttgagaaagg aaagcctttc tttgagaaat tatcgagaaa tatatactta agagcgataa
     5101 aagatggttt tatttccagt atgccagcag tattattctc aagtattttt atactaatag
     5161 ctgctgtgcc caatattttt gggtttaaat ggtctgatga acagttagct tttattttga
     5221 aaccttacaa ctactcaatg gggattttag ctctcttagt agctgggaca actgcaaaat
     5281 cgttaacaga ttcagttaat acacgtagta tggagaaaac caaccaaatc aactacatgt
     5341 caactttttt agcagctgtt gttggattgt taatactggc agctgatcca attgaaggtg
     5401 gatttgctaa tggtctttta ggtactagag gattacttac tgcatttttg gcagctttta
     5461 ttacagtaaa tatctataaa gtatgtatta aaaataatgt aaccattaga cttcctgaag
     5521 aggttccacc aaatattgca caagttttta aagatgttat tccgtttgcc ttatctgttc
     5581 tttcaatata tggattggat ttaattgtta gaaatatttt tggtacaaat gtagcagaat
     5641 ctgttggtaa aatattagcc cctctatttt cagctactga tggttatatc ggtttagcta
     5701 ttgtatttgg tgcttatgct ttcttttggt ttgttggaat tcatgggcca tctgttgtag
     5761 aacctttaat tgtagctatc agttatgcaa atatagaagc taatgttcag cttgtgcaag
     5821 ctggtatgca cgcagataaa attttaaatc cagttactca aacttttgtt gtaacaatgg
     5881 gtggtactgg tgcaacacta gtagttccat ttatgtttat gtggttatgt aaatcaaaaa
     5941 gaaatagaat tgtcggacgt gcgtctgtag ttcctacttt ctttggcgta aatgaaccaa
     6001 tcctatttgg tgctccaatt gttttaaatc ctatcttctt tattccgttt gttactgctc
     6061 ctattatcaa tgtttggatt atgaaatttt ttgtagatgt tctacaaatg aatagtttca
     6121 gtattatttt accttggaca acacctgctc caattggtat tgttatggga acggcgctgg
     6181 ctccattgtc attcgtcttg gctataactt taatcattat cgacacttta atctattatc
     6241 catttgttaa agtttatgac catcaaattt tagaagaaga acggaaagga aactcttcat
     6301 ctgaattgaa agaaaaagtt gctgcaaact tcaacactgc aaaagcggat gctattcttg
     6361 aaaaagcggg tgtagaagca gcacaaaata caatcactaa agaaacaaat gtcctcgttc
     6421 tctgtgcagg tggaggaaca agtggtctcc ttgcaaatgc tttgaataag gcagcagcag
     6481 aatacaatgt ccctgtgaaa gcagcagcag gcggctatgg tgctcaccgt gaaatgttac
     6541 cagagtttga tcttgttatc cttgcccctc aagttgcttc aaactttgaa gatatgaaag
     6601 cagaaacaga taagctcggt attaaactag cgaaaacaga aggcgctcaa tacatcaaat
     6661 taactcgtga tggaaaaggt gctcttgcat tcgtacaagc gcaattcgat taaggctaga
     6721 gactctgaaa tagtctccta tcgttacgga aatcgctatg gcgaatttcc tattattaat
     6781 tcgtcggtaa aaagatatcg tttttacctc ctcatgtcac aattcggtga cttggtacaa
     6841 gaagtgagat ggagaaggat ggctcactga ctcctctcct ctcactttta ctttatttaa
     6901 atcaagaaat aggtgaaaaa aatgacaaaa acacttccaa aagactttat ttttggtggc
     6961 gcaacagctg cctatcaagc agaaggtgct acacatactg atggtaaagg accagtagcg
     7021 tgggataaat atcttgaaga taactactgg tacactgcag aaccagccag tgatttttac
     7081 aatcgatatc cagttgacct caagctagca gaagagtatg gtgtcaatgg tattcgaatt
     7141 tctattgctt ggtcacgtat tttcccgact gattacggcc aagtaaatgc taaaggtgtt
     7201 gagttttatc ataatttatt tgcagagtgt cacaaacgtc atgttgagcc ttttgtaact
     7261 cttcatcact ttgacacgcc agaagctctc cactcaaatg gagacttctt aaaccgtgaa
     7321 aatatcgaac attttgtaga ctacgctgcc ttctgttttg aagaatttcc agaagtaaac
     7381 tattggacaa cctttaatga aattggacca atcggtgatg gtcaatattt ggttgggaaa
     7441 ttccctccag gtatccagta cgaccttgcc aaagtctttc aatcacacca caatatgatg
     7501 gtgtctcatg cacgcgcggt aaaattgtac aaagagaaag gctataaagg ggaaattggt
     7561 gttgttcacg ccctgccaac taaatatcct ctagatcctg aaaatccagc agatgttcgt
     7621 gcagctgagt tggaagatat catccacaat aaattcatct tagacgcaac ttatctaggt
     7681 cgctattcag ctgaaaccat ggaaggtgtc aaccatatct tatcagtcaa tggtggtagc
     7741 ttggatcttc gtgaagaaga ttttacagca ttagaagctg caaaagactt gaatgatttc
     7801 ctaggaatca actactatat gagtgactgg atggaagcct ttgatggaga aactgaaatt
     7861 atccataatg gtaaaggtga aaaaggaagc tctaagtatc aaatcaaagg tgttggtcgt
     7921 cgtgtagctc ctgactatgt accacgcacg gattgggatt ggattatcta ccctcaaggt
     7981 ttgtatgacc aaatcatgcg tgtgaagaaa gattatccta actacaagaa gatttacatc
     8041 actgaaaatg gtctcggcta taaagatgag ttcgttgata acactgttta cgatgatggt
     8101 cgtattgatt acgtgaaaca acacttggag atcttgtctg atgcgattgc agatggagct
     8161 aatgtaaaag gttacttcat ttggtcatta atggatgtct tctcatggtc aaacggttat
     8221 gagaaacgtt atggtctctt ctacgtagat tttgaaactc aagaacgtta tcctaagaaa
     8281 tcagctcact ggtacaagaa agtagcggaa actcagatta tagactagta gaattagtca
     8341 ttagatatag aattttagtg agtcaaaaag atgttcaaag attttatcca atctatttat
     8401 gaaaaaaagt ttatattata aatttcgaaa aatgctctca aataccgtgt ttgacgagtg
     8461 aagaattgaa aagtcttgga aaatggtatg tctcgactgg taaagaatgg atttgtcatt
     8521 cagatgatga gctggaagaa tttaaaaatc tatttttaaa ttttatcaat cctgaagaat
     8581 gggatactat ctcctttgat tcagatttta tgccgtttca acaatcgtaa ccaatttctc
     8641 aaaaaagtta aatcttatat ttagtactct gtaaaactct tatctaatca cgttgcttat
     8701 actcaatgaa aatcaaagag caactttaaa ctaggaagcg agtcgcagat ttctcaatgc
     8761 atagctttga ggaattgggc aaaaagtctt tgatatagaa aaaagcatag tatcaggtgt
     8821 tgaaacacct gatactatgc tttttattgt gggaagattt actttttttc ttctgaaatt
     8881 gagttgttac ccaggctctt tcagtttatt aaggcttgat gactttaatg tgtttagata
     8941 gcttaaaaag gattgaatca cttagtttag aatctgaaac aatagtatca agatttgata
     9001 tattataaaa agtataaaaa tcaaacttat tgaacttact atgatctgcg agtaaatatt
     9061 ttttattaga attatttaaa gcgatgcgtt gagcctctcc ctcttcctcg ctaaaagtag
     9121 ctagagctcc gttttgaata ccattacagc taacgaaagc tttagaaaat tggagattag
     9181 agagattttg tagggtcaat gtgccaacaa aagcacctgt aatatcgcga taatttccac
     9241 ctatcaaaat caaatctgtt aattttcgtt cgcttaaaat cagaaaaaca ggtagactgt
     9301 tggttacgac gcggatattg tcaataggca actcacgcgc aaaaaactct aatgttgttc
     9361 ctggtccaat gaaaatagtt tctctttctt ctactagact gcctgcaaaa tgggctattt
     9421 cttgtttttc tgccgtttgg agggcttgtt tttcaatatt tgatcgctca ttagtcaaaa
     9481 gggagttggt tcgaagtttt tcagctccac catgcacacg aatcagcaaa tctttatcag
     9541 ctaattcctg taaatagcgc cttgcagtca tatctgaaac ggctatttcg tccataatct
     9601 gtttaactgt tatggttcct ttactattta ctatctctaa aattttggtt aatttttctt
     9661 gtttgagcat attatcacct cgtttcctac tactatctta ccataaacaa actcatcatt
     9721 caaatacaaa aacaacaaaa tgaaacaaaa acaaaaatat cgaagtttgt tttcaaaact
     9781 ttcgatattt ttgttgggtt attattttgt tgtttctagt ttactttttg atggttaaga
     9841 gtggtggaga at
//
LOCUS       DAVMMR010000063         9520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMMR010000063 DAVMMR010000000
VERSION     DAVMMR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.063"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..219
                     /locus_tag="VYS74_001719"
     CDS             <1..219
                     /locus_tag="VYS74_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3789720.1"
                     /translation="NSKPQADDKKPSTTNNLSKDKQPSNQASTNEKATNKPKKSLPST
                     GSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VYS74_001720"
     CDS             complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VYS74_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV3789721.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VYS74_001721"
     CDS             complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VYS74_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV3789722.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2442..3869
                     /locus_tag="VYS74_001722"
     CDS             2442..3869
                     /locus_tag="VYS74_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3789723.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4028..5536
                     /gene="glpK"
                     /locus_tag="VYS74_001723"
     CDS             4028..5536
                     /gene="glpK"
                     /locus_tag="VYS74_001723"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV3789724.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5578..7404
                     /gene="glpO"
                     /locus_tag="VYS74_001724"
     CDS             5578..7404
                     /gene="glpO"
                     /locus_tag="VYS74_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV3789725.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7474..8178
                     /locus_tag="VYS74_001725"
     CDS             7474..8178
                     /locus_tag="VYS74_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3789726.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8369..8572
                     /locus_tag="VYS74_001726"
     CDS             8369..8572
                     /locus_tag="VYS74_001726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789727.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8659..8919)
                     /locus_tag="VYS74_001727"
     CDS             complement(8659..8919)
                     /locus_tag="VYS74_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789728.1"
                     /translation="MTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(8977..9176)
                     /locus_tag="VYS74_001728"
                     /pseudo
     CDS             complement(8977..9176)
                     /locus_tag="VYS74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2764 a         1859 c         2158 g         2739 t
ORIGIN      
        1 aatagcaagc cacaagcaga tgataagaag ccatcaacta caaataattt aagcaaggac
       61 aagcaacctt ctaaccaagc ttcaacaaac gaaaaagcaa caaataaacc gaagaagtca
      121 ttgccatcaa ctggatctat ttcaaatcta gcacttgaaa ttgcaggtct tcttaccttg
      181 gcgggggcaa ccattcttgc taagaaaaga atgaaatagt gattgattca ttcaaaaatg
      241 tttgtaaaaa gtattcggct taaaagaaaa gagtctggga taaaaagatt ttgattttag
      301 gaattcttta ttataaattt ttaaaatcga tagattagac aaaaaagcga acaagacaga
      361 attctgagtg tcagataact gattttgttc gctttttata tttaatatta tacttttgtc
      421 ctaggctctt ttttgcatta tactttttcc aattgcaaga gggcttcaat ctctgccagg
      481 gtgctagctt gcgaaatggc tccacggagt ttggcagcgc cagatgttcc ccggagatag
      541 tgaggagcga ggccgcggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat
      601 cgtttcaagt gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt
      661 tctcctgttt caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct
      721 cggccaatca tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca
      781 gtacggatat caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtaa
      841 agggtctcaa ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg
      901 agggcagaaa cacctgcagc ctcagcagcg agggcatttt ctactgcaag agatgggtcc
      961 gcccagccgg tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg
     1021 atgatagagt agatcttgtc aggatccttg agccacatag caccagcttc gttcttcacg
     1081 attttgttga cagggcagcc catgttgata tcgacgatat cggtcttagt gttttcttgg
     1141 atgaattctg ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca
     1201 gggttttcgc cctcatcgat atgaagcata tgcagggttt tttcgttgtt gtattggatt
     1261 cccttgtcag agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta
     1321 cgaaaggctg agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca
     1381 atattgccaa tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt
     1441 ttcatcaaag ttgtaagtag tttggcagaa ttgacaagtg atttctgccc cgtggtcttc
     1501 ctctttcatt tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc
     1561 atggctacag tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc
     1621 cccgtagata gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat
     1681 agctggcatt tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg
     1741 caagacttga actaggaaac cacctgcaac cttgaccttg tcttcctcgt ccaaaaggac
     1801 attgaggccg accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc
     1861 accgatttct ccagagatga ggggagttat agagttgtaa ggatttccag taccgtagtc
     1921 tgtgataacg aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc
     1981 agtctttttg atgtcaacac caggattttg aacatagcct ttgacgttcc ccttggtatc
     2041 agcgacggtg ataatagcac ctagagagct agagcccaac accttaactg taagtttggt
     2101 atttcctttt tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac
     2161 agttgagcta gcttgggttt gatgtttttc ttgagcagtg cggacggttt ccgtgctatc
     2221 aaggacaaaa gcacgaaagg ctccgctttc tgatatagtt ttaataattt tatccatagc
     2281 tactatttta gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata
     2341 atggaccagg aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag
     2401 atttatgcta tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga
     2461 tggagaaagc cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga
     2521 cgaccgtcaa ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg
     2581 tcaccctgct caatgacaag gctttggata gtggcttaga gctggctatt cactcagaag
     2641 atgaaaatct gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt
     2701 ttttggagag tgctgttaaa taccagattt tggtttatct tctctaccac caacagtttt
     2761 tagcccatca gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg
     2821 ctggtttaaa tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag
     2881 gtccagagca tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc
     2941 aggaatggga aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg
     3001 aaatctgcgg tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca
     3061 tcagtcaaca acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc
     3121 gagggtattt tgacaatatt ttctaccttc gtttgctgag aaaggttccg tccttttttg
     3181 ctgggcaaca tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc
     3241 tcctatctca tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc
     3301 agttggcaga tttactgacg caattgattc aagaaatgaa gaaggaggaa ctattggggg
     3361 attatacaga ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct
     3421 ataagggcta tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt
     3481 tactgatgga acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt
     3541 ttcaacaggg gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat
     3601 atatggctga aaacggtggc cagcatatgc ggattggtct ggatttgaca tctggttttc
     3661 ttgtcttttc aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta
     3721 ccattgaagc ttatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc
     3781 ataagaagga acagacacca gtctattatt taaaaaacga cttggatatg gaagatttgg
     3841 cagggattcg tcagttatta ttcacttaaa aggcttggtt aatctaggtc ttttttgtga
     3901 aattcacaca atctcctcac atttttttaa aaattaaaaa aagttgataa acaagaaagc
     3961 gctttatttt gtatactagt aagtgtaaag aggaaacacc tcaagatctt tatcaggagg
     4021 acagtacatg tcacaagaaa aatacatcat ggccattgac cagggaacta caagttctcg
     4081 tgccatcatt ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag agtttaccca
     4141 gattttccct caggcaggtt gggttgagca caatgccaat gaaatttgga actctgttca
     4201 gtcagttatt gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa tcgaggcaat
     4261 cgggattacc aaccaacgtg aaacaacggt tgtctgggat aagaaaacag gacttcctat
     4321 ctacaatgct atcgtttggc agtcacgcca gacagcacct ttggctgagc aactaaaaag
     4381 ccaaggttat gtggaaaaat tccatgaaaa gactggtttg attattgatg cttacttctc
     4441 tgctaccaag gttcgttgga ttttggatca tgtagaaggt gctcaagagc gagcagaaaa
     4501 aggggaattg ctctttggta ctatcgatac ttggttggtt tggaaattga ctgacggtgc
     4561 ggctcacgtg actgactact caaatgcagc tcgtaccatg ctttataaca ttaaagaact
     4621 caaatgggat gatgagattt tggaaatcct taacattccg aaggctatac ttccagaagt
     4681 tcgttctaac tccgaaatct acggcaagac agctccattc catttctacg gtggagaggt
     4741 gccaatctca ggtatggctg gggaccaaca agcagccctc tttggacagt tggcttttga
     4801 gccaggtatg gttaagaata cttatggaac aggctctttc atcatcatga atactgggga
     4861 agagatgcag ttgtctgaaa acaacctctt gacaaccatt ggttacggaa tcaacggtaa
     4921 ggtttattat gccttggaag gttctatctt catcgcagga agtgctattc agtggcttcg
     4981 tgacggtctt cgcatggttg aaaattcacc agaatctgaa aaatacgctc gtgattctca
     5041 caacaacgat gaagtttatg tcgttccagc ctttacaggt ctaggcgctc catactggaa
     5101 ccaaaatgct cgtggttccg tctttggttt gactcgtgga acaagcaaag aagactttat
     5161 caaggcgact ttgcaatcta ttgcttatca agtgcgtgat atcatcgaca ccatgcaagt
     5221 ggatactcag accgccattc aagtactgaa ggtggatggt ggtgcagcca tgaacaactt
     5281 cctcatgcag ttccaggcgg atattttagg cattgacatt gcacgtgcta aaaacctgga
     5341 aacaacagct ctaggagcgg ccttcctagc aggtttgtca gtagggtact ggaaagactt
     5401 ggacgagttg aaactcttga acgagacagg agaactcttt gagccatcta tgaacgaatc
     5461 tcgcaaggaa caactctaca agggctggaa gaaggctgtg aaagcaactc aagtctttgc
     5521 ggaagtagac gactaatact ggcagaataa agcgatttat ttagaaagtg tgtaaatatg
     5581 gaattttcaa agaaaacacg tgaattgtca attaaaaaaa tgcaggaacg taccctggac
     5641 ctcttgatta tcggtggagg aatcacagga gctggtgtag ccttgcaggc ggcagctagc
     5701 ggtcttgaga ctggtttgat tgaaatgcaa gactttgcag aaggaacatc tagtcgttca
     5761 acaaaattgg ttcacggagg acttcgttac ctcaaacaat tcgacgtaga agtggtctca
     5821 gatacggttt ctgaacgtgc agtggttcaa caaatcgctc cacacattcc aaaatcagat
     5881 ccaatgctct taccagttta cgatgaagat ggagcaacct ttagcctctt ccgtcttaaa
     5941 gtagccatgg acttgtacga cctcttggca ggtgttagca acacaccaac tgcgaacaag
     6001 gttttgagca aggatcaagt cttggaacgc cagccaaact tgaagaagga aggcttggta
     6061 ggaggtggag tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat tgaaaacatc
     6121 aaacgtgcca accaagacgg tgccctcatt gccaaccacg tgaaggcaga aggcttcctt
     6181 tttgacgaaa gtggcaagat tacaggtgtt gtagctcgtg atctcttgac agaccaagta
     6241 tttgaaatca aggcccgtct ggttattaat acaacaggtc cttggagtga taaagtacgt
     6301 aatttgtcta ataagggaac gcaattctca caaatgcgcc caactaaggg agttcacttg
     6361 gtagtagatt caagcaaaat caaggtttca cagccagttt actttgacac aggtttgggt
     6421 gacggtcgta tggtctttgt tctcccacgt gaaaacaaga cttactttgg tacaactgat
     6481 acagactaca caggtgattt ggagcatcca aaagtaactc aagaagatgt agattatcta
     6541 cttggcattg tcaacaaccg ctttccagaa tccaacatca ccattgatga tatcgaaagc
     6601 agctgggcag gtcttcgtcc attgattgca gggaacagtg cctctgacta taatggtgga
     6661 aataacggta ccatcagtga tgaaagcttt gacaacttga ttgcgactgt tgaatcttat
     6721 ctctccaaag aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct tgaaagtagc
     6781 acatctgaga aatatttgga tccatctgca gtttctcgtg ggtctagctt ggaccgtgat
     6841 gacaatggtc ttttgactct tgctggcggt aaaatcacag actaccgtaa gatggctgaa
     6901 ggagctatgg agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag ctttaaattg
     6961 atcaattcta aaacttaccc tgtttcaggt ggagaattga acccagcaaa tgtggattca
     7021 gaaatcgaag cctttgcgca acttggagta tcacgtggtt tggatagcaa ggaagctcac
     7081 tatctggcaa atctttacgg ttcaaatgca ccgaaagtct ttgcacttgc tcacagcttg
     7141 gaacaagcgc caggactcag cttggcagat actttgtccc ttcactatgc aatgcgcaac
     7201 gagttggctc ttagcccagt tgacttcctt cttcgtcgta ccaaccatat gctctttatg
     7261 cgtgatagct tggatagcat cgttgagcca gttttggatg aaatgggacg attctatgac
     7321 tggacagaag aagaaaaagc aacttaccgt gctgatgtcg aagcagctct cgctaacaac
     7381 gatttagcag aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag cattccttgt
     7441 cgctcgtccc ttctttttaa tggagacaga aagatgatga atgaattatt tggagaattt
     7501 ctagggactt taatcctgat tcttctagga aatggtgttg ttgcaggtgt ggttcttcct
     7561 aaaaccaaga gcaatagctc aggttggatt gtgattacta tgggttgggg gattgcagtt
     7621 gcggttgcag tatttgtatc tggcaagctc agtccagctc atttaaatcc agctgtgaca
     7681 attggtgtag ctttaaaagg tgacttgcct tgggcttccg ttttgcctta tatcttagcc
     7741 cagttcgcag gggctatgct gggtcagatt ttggtttggt tgcaattcaa acctcactat
     7801 gaggcagaag aaaatgcagg caatatcctg gcaaccttca gtactggacc agccatcaag
     7861 gatactgtat caaacttgat tagcgaaatc cttggaacct ttgttttggt gttgacaatc
     7921 tttgctttgg gtctttacga ttttcaggca ggtatcggaa cctttgcagt gggtaccttg
     7981 attgtcggta tcggtctatc gctaggtggg acaacaggtt atgccttgaa tcctgcgcgt
     8041 gaccttggac ctcgtatcat gcacagtatc cttccaattt caaacaaggg agacggagac
     8101 tggtcttatg cttggattcc tgttgtggga cctgttatcg gtgcagcctt ggccgtgctt
     8161 gtattctcac ttttctaatc tagaaaacaa ttatgttgat agagcttggg caagagccca
     8221 atttcagcaa aaaatgaagt aaatcttctc ataataaaac gcatcatatc aagcacgaaa
     8281 attccacgag gtcaactaca gtcagaaagc tgaacaacaa gccaaaacgc ccaaaaaagg
     8341 cggcaaaaag caagcacctg caagcaacgt gccgaaatgg tcaaatcctg attatgtcaa
     8401 cgaattagac ccaaaaatcg ttgatatgct agtagaattt cacaagtcac aaggcacttt
     8461 ggaaactccc gaggcgcaag cagaaatcgc ccaaaaacgt gaagaaatcg agcaaaggag
     8521 agctgagctt gaggataaaa aacaagagct tttgaaccgc ttgaacaaat agagtttcgc
     8581 aagtattatg cttacaaatt acttgagcaa ttaactaaaa tataaaccct gcctttatat
     8641 ctaggcaggg tttatatttt agaaattcac gtaggttgtt acggttttta catacccagt
     8701 atagtttgag tttctatagt attcagtgat aaacttccat tttctttgag caacatggat
     8761 ataagtactt gttatgtagt atggatatgg gctttgtgaa tccaagtaag actgataagc
     8821 ttgtatacca aaatatgctc caccaattat tgcaccccat ggacccccca ataaagcacc
     8881 tatcctacca atcatataac tgattccagc accagtcatg aagttagcga atgtgttagc
     8941 ttgtttattc ccatgtattg tgttgacgta attccaaaca ttaggatcgt atgatctaaa
     9001 agatatattt aggtcgattt cattcttttg ataagccata taaaatgccc cattgatata
     9061 gacgccgtca gcacgtcgtt caatagtgtc tacacttcca tctggattga cgaaatgcta
     9121 gacatacaga aaacagacat aactagcaaa cacatgcatt taaacatctt agacataacg
     9181 gaaactcctt tgtatttttg atttttttta acttttatta tacaataaaa ccaaataaaa
     9241 agaaagcggt aacaatatgc ttaatgcgaa aattttttat atatttttat gtttgatcgt
     9301 tatcgaaact acaggcttgt tgttgttgaa aagaggtctc gaaatgggtt atttagacac
     9361 agaagctatt atcctcgcag ttttttcatt tgctttttac aacctatgtt cattcgcttg
     9421 ggtctgctct acaataaaaa ataaatagac gtattttcaa aaaaaaaaaa aatgcatatt
     9481 tatcttagca aatcgacgat ttaaatcgtc gtttttttgt
//
LOCUS       DAVMMR010000064         9424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMMR010000064 DAVMMR010000000
VERSION     DAVMMR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.064"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            127..990
                     /locus_tag="VYS74_001729"
     CDS             127..990
                     /locus_tag="VYS74_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789729.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1373..1993
                     /locus_tag="VYS74_001730"
     CDS             1373..1993
                     /locus_tag="VYS74_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789730.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2079..2522
                     /gene="tsaE"
                     /locus_tag="VYS74_001731"
     CDS             2079..2522
                     /gene="tsaE"
                     /locus_tag="VYS74_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV3789731.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVVILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2512..3030
                     /locus_tag="VYS74_001732"
     CDS             2512..3030
                     /locus_tag="VYS74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789732.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEATEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            3038..4054
                     /locus_tag="VYS74_001733"
     CDS             3038..4054
                     /locus_tag="VYS74_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3789733.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4138..5394
                     /locus_tag="VYS74_001734"
     CDS             4138..5394
                     /locus_tag="VYS74_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV3789734.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5449..6615
                     /gene="recA"
                     /locus_tag="VYS74_001735"
     CDS             5449..6615
                     /gene="recA"
                     /locus_tag="VYS74_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV3789735.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6922..8292
                     /locus_tag="VYS74_001736"
     CDS             6922..8292
                     /locus_tag="VYS74_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3789736.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8668..>9424
                     /gene="lytA"
                     /locus_tag="VYS74_001737"
     CDS             8668..>9424
                     /gene="lytA"
                     /locus_tag="VYS74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV3789737.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFN"
BASE COUNT         2861 a         1604 c         2089 g         2870 t
ORIGIN      
        1 atttttatgt aatttttgac tttttttagt ttattaccat aaagaattat ttatttcttt
       61 aacttgctat tttgaactaa aaattttata atgcaattaa gcaaatagga aggtttatta
      121 tctttaatga gtacactcgc agagaaattc agattgaaaa gaaaagagtt gagactctcc
      181 caacaaactc ttgcagaagg aatttgtgaa caaagccaga ttagtaaaat tgagagaggg
      241 catttcattc cctccgcaga ccttttgttc aaactctctc aacgacttga agtaccatta
      301 gattattttt ttaatgaaca aattgaaatt aaatctaacc tctctaattt caagcaattg
      361 tctgctcgac tattagacga tagaaattat gacgatttgg aatatattta tagaatagag
      421 attgaacgaa gtacttttct aacactagaa gaccgaactt accttgaatg gattcaagct
      481 attattgact tctatcaata tgacagtaag tgtgaggcaa tttcttcatt ggaaaatata
      541 ttattaaaag tctcttcaaa tactctgatt tatttaaagg cgttgaatac tctatctaat
      601 ttctattcct tagtgggtcg tgaacaagaa tatgaggcaa actactctca tttaatagag
      661 ttgtatcaga caaaaaattt ggaccatcaa gagtttttat ttggctacat cagagttcgt
      721 tacaactacg ctcactacct agtatcaaag gaaaaatata atgaagccat ccaagaagct
      781 cttgaaacga ttgaactctg taaacaaaga cagacaagct accaattggc tcctctactt
      841 attcttgtag gaaatgctgg agccaaattt ctagacagag aacaagtcaa aaattattat
      901 atagaagcaa gagagttatg taagatttat aacaatcctt taatgttgat gaagatagaa
      961 aattatttga aggaactaga tactgtttag ttaatcttga cattatagtt tttctgaaac
     1021 gttaaataac ctgtaaggct gatagttaga ttaatactat cagccttata attatgtgac
     1081 tctgagtccg tctcgctccg tgaggtgcga gacaaaacaa ccacccgttt gacggatttt
     1141 catgatttca aatttatatt cttcgaaaat ctcttcaaat cacgtcagct ctatctgcaa
     1201 tctcaaaaca gtgttttgag caacctgtgg ctagctacct agtttgatct ttgattttca
     1261 ttgagtatta gtctctagtg tttgtttagc gtcttaaaga gacatttgag aagattagaa
     1321 acagttctct cttttctcct agtataatga ttggtaggaa gggagagaga agatgaaatc
     1381 aatgagaatc ttatttttgt tagctttaat tcaaatcagt ttgagtagct gtttcttatg
     1441 gaaggaatgc atcttgtcct ttaaacaaag tacagctttt ttcatcggaa gcatggtttt
     1501 cgtttcagga atctgtgctg gagtaaatta tctttatacc cgtaagcaag aagtccatag
     1561 tgtcctagcc agtaagaagt cggtgaagct tttttacagt atgttactct taattaattt
     1621 gttaggagct gttcttgttt tgtcagataa cttgttcatc aaaaatacgc tgcagcaaga
     1681 attagttgac tttttattgc catccttctt tttcctattt gggctagatt tgctgatttt
     1741 tttacccttg aaaaaatacg tgcgcgattt tcttgctatg ctagacagaa aaaagacagt
     1801 gttggtgact attttagcaa cacttctttt cttaagaaat ccaatgacca ttgtctcact
     1861 tctgatttat attggactgg gcttgttttt tgcagcctat cttgtcccaa attcggttaa
     1921 gaaggaagtt tccttttatg gtcatatttt ccgagatctt gtattggtca ttgttacgct
     1981 cattttcttt tagagaaagt ctgtttttta tggatgttat gatgataagt ttagtaggaa
     2041 aatagacaca aaagaaaagt ttttggtata ataagagtat gtacacaaaa aatgaagaag
     2101 agttgcaagc cttaggggag cgtttgggcc atctattagc aaagaatgat gttgtaatct
     2161 taactggaga actgggtgca ggtaaaacga cctttactaa aggacttgca aaaggattac
     2221 agatttctca aatgattaaa agtcccacct atactatcgt gagagagtat gaaggtcgac
     2281 ttccacttta tcacctagat gtttatcgta ttgaaggaga tgctgattct atcgacttgg
     2341 atgagtttat ctttggtggc ggcgtgactg ttattgagtg gggaaatctc ttaggagatg
     2401 ccttgccaga tgcttattta gaattggaaa ttctaaaaga agcagatgga cgccgtttaa
     2461 attttcaggc aaagggtttg cgtgctgaga aattgttaga ggagcttcaa tatggagtat
     2521 gaattgctca ttagggaagc agagcccaaa gatgcagctg aattagtggc ctttttaaat
     2581 cgtgtgagtt tggagacaga ctttaccagc ctagacggag atggtattct cttgactagt
     2641 gaggagatgg aaatattcct caacaagcaa gctagttcgg acaatcagat aaccttactt
     2701 gcattcttaa atggcaaaat tgctggtatt gtaaatatta cagctgatca gcgtaagaga
     2761 gtccgtcata ttggagatct cttcattgtg attggaaaaa gatattggaa taatggcttg
     2821 ggaagtttgt tgctagaaga agcgacagag tgggcacaag caagtggcat tctgcgtcgt
     2881 ctccaactga ctgtccaaac tcgtaatcaa gcagcagtcc atctttatca aaagcatggc
     2941 tttgtcattg aaggtagcca agagcgtggg gcatatatag aagaagggaa atttatcgat
     3001 gtttacttga tgggtaaact gataggttag tagaaatatg gttaaaaaaa ttattggaat
     3061 ggtgctagct ttactttctg taactgtagt aggagtaggt gtttttgctt atactattta
     3121 tcaacaaggg acagaaacct tagctaaaac ctataaaaaa atcggcgaag aaaccaaggt
     3181 tattgaagcg actgaacctc taaccattct gttaatggga gtggacaccg gaaatgctga
     3241 acgaactgaa acttgggtcg gtagaagtga tagcatgatc ttgatgacag tgaatcctaa
     3301 aacgaaaaaa acaacaatga tgagtttaga gcgggatatt ctgacgcgca ttgaatcagg
     3361 gaatggtcag gctcatgaag cgaaactgaa ctcagcatat gcagatggtg gagcagagct
     3421 tgctatagaa accattcaaa aaatgatgaa tatccatatt gatcgctatg tgatggtcaa
     3481 tatgagaggg ttgcaaaaac tagtggatgc agtaggaggt attacagtca ataatatcct
     3541 aggtttccca atttctatca gtgaccaaga agaatttaat actatttcta tcggtgttgg
     3601 ggagcaacat attgggggag aagaagccct agtctatgca cgaatgcgtt accaagatcc
     3661 tgagggggat tatggtcgtc aaaaacgtca acgtgaagtt attcaaaaag tcatggaaaa
     3721 agctctcagt ttaaatagcg ttggtcatta tcaagagatt ctaaaagctt tgagtgacaa
     3781 tatgcagacc aatattgatt tgtctgcaaa aagtatccct aacttgctag gctataaaga
     3841 ttcatttaaa accattgaaa ctcagcagtt gcagggtgaa ggagagatac ttcaaggtgt
     3901 ttcttaccag attgtttcga gagcacatat gttggaaatg caaaatatac tccgacgttc
     3961 tttgggacaa gaagaagtta ctcagcttga aaccaatgcg gttttatttg aagatttatt
     4021 tggcagagca cctgttggtg atgaagataa ttaacttttg atacaaataa aaaatcaatc
     4081 gtaggaaaat tcctgcgatt ttttcaaaaa aatacgaata gataggtagg aggaaacatg
     4141 aaagcagaaa tcattgctgt tggaacagag attttgacag gacagattgt caacaccaat
     4201 gcccagtttt tgtcggaaaa actagctgag attggggtag atgtatattt tcagacggct
     4261 gtaggagaca atgaagttcg tctcttgtct ttgcttgaga ttgccagtca acgtagcagt
     4321 ctggtgattt tgacaggcgg tttggggcca actgaggacg acctaaccaa acaaacccta
     4381 gctaaatttt tagggaaagc attagtcttt gatcctcagg ctcaggagaa gttggatatc
     4441 ttttttaccc tgcgaccaga ctatgcccga acaccaaata acgaaagaca agctcaaatt
     4501 gtagaaggag cgattccact gccaaacgaa acaggactgg ctgttggagg aaaattagaa
     4561 gtagacggag tgacctatgt cgtccttcca ggtccgccaa gtgaattgaa acccatggtc
     4621 ttaaaccaac ttctacccaa gttgatgaca gggagcaagc tgtattcccg agttcttcgt
     4681 ttctttggga ttggcgagag ccagttggtt acgattttgg ctgatttaat tgataatcag
     4741 atcgatccta ccttggcccc ttatgccaag acaggagaag tcactctacg tctgtcaaca
     4801 aaggctagca gtcaagaaga ggcgaatcaa gcgctggata tcttggaaaa tcaaatcttg
     4861 gactgccaga ctttcgaagg aatttcttta cgagactttt gctatggtta tggggaagaa
     4921 actagtttag ccagcattgt ggtagaagaa ctgaaaaggc aagggaaaac catcgcggct
     4981 gcagagagtt tgacggcagg tcttttccaa gctaccgtgg cgaatttttc tggagtttca
     5041 agtatatttg agggtggttt tgtgacctat agcttggagg aaaaatcaag gatgttggat
     5101 attcctgcca agaatttgga agaacatggt gtggtgtctg aatttacagc tcagaagatg
     5161 gctgagcagg cacgaagcaa gacccagtct gattttggaa ttagtttgac tggagtggca
     5221 ggaccagata gcctagaagg acacccagtt gggacagtct tcataggctt ggcgcaagat
     5281 caaggaactg aggttatcaa ggttaatatt ggaggcagaa gccgagcaga tgtacgtcac
     5341 attgcggtta tgcatgcctt taacctagtt cgcaaggctt tattaagtga ctaacttttg
     5401 atataatagt agataggtct gaggatcatt agaatgtagg agaatagaat ggcgaaaaaa
     5461 ccaaaaaaat tagaagaaat ttcaaaaaaa tttggggcag aacgtgaaaa ggccttgaat
     5521 gacgctctta aattgattga gaaagacttt ggtaaaggat caatcatgcg tttgggtgaa
     5581 cgtgcggagc aaaaggtgca agtgatgagc tcaggttctt tagctcttga cattgccctt
     5641 ggctcaggtg gttatcctaa gggacgtatc atcgaaatct atggcccaga gtcatctggt
     5701 aagacaacgg ttgcccttca tgcagttgca caagcgcaaa aagaaggtgg gattgctgcc
     5761 tttatcgatg cggaacatgc ccttgatcca gcttatgctg cggcccttgg tgtcaatatt
     5821 gacgaattgc tcttgtctca accagactca ggagagcaag gtcttgaaat tgcgggaaaa
     5881 ttgattgact caggtgcagt tgatcttgtc gtagtcgact cagttgctgc ccttgttcct
     5941 cgtgcggaaa ttgatggaga tatcggagat agccatgttg gtttgcaggc tcgtatgatg
     6001 agccaggcca tgcgtaaact tggcgcctct atcaataaaa ccaaaacaat tgccattttt
     6061 atcaaccaat tgcgtgaaaa agttggagtg atgtttggaa atccagaaac aacaccgggc
     6121 ggacgtgctt tgaaattcta tgcttcagtc cgcttggatg ttcgtggtaa tacacaaatt
     6181 aagggaactg gtgatcaaaa agaaaccaat gtcggtaaag aaactaagat taaggttgta
     6241 aaaaataagg tagctccacc gtttaaggaa gccgtagttg aaattatgta cggagaagga
     6301 atttctaaga ctggtgagct tttgaagatt gcaagcgatt tggatattat caaaaaagca
     6361 ggggcttggt attcttacaa agatgaaaaa attgggcaag gttctgagaa tgctaagaaa
     6421 tacttggcag agcacccaga aatctttgat gaaattgata agcaagtccg ttctaaattt
     6481 ggcttgattg atggagaaga agtttcagaa caagatactg aaaacaaaaa agatgagcca
     6541 aagaaagaag aagcagtgaa tgaagaagtt acgcttgact taggcgatga acttgaaatc
     6601 gaaattgaag aataagctgt taaagcagtg gagaaatccg ctactttttc gatttttgat
     6661 tcaagttttt agattatata tagtagcttg aaataagata tgaacaactt tattaggaaa
     6721 gtcaaattaa tttctagaaa tgttttagca gctacagcgt actattccaa actcaaccaa
     6781 ctataataga tcgaaactag aatagtacac atctacttct aaaacattgt taaaaatcga
     6841 tttgactgtc cttatttcat tccgctatat ataagtttgc tgtttcttgt cgctcctctg
     6901 gaaagctgat ataatagctt tatgaataaa aaacgaacag tggacctgat acatggtccg
     6961 attcttctct cgctcttaag cttcgccttc ccaatcttgc tatcaaatat ttttcaacag
     7021 ctctataaca ctgctgatgt cttgattgtt ggacgatttc ttggtcaaga atccttggct
     7081 gcagtaggag cgacgacagc gatttttgac ctgattgtag gttttacact tggtgttggc
     7141 aatggcatgg ggattgtcat tgctcgttat tatggggctc ggaatttcac taaaatcaag
     7201 gaagcagtag cagccacctg gattttaggt gctcttttga gcattctagt tatgttgctg
     7261 ggctttcttg gcttgtatcc tctcttgcaa tacttagata ctcctgcaga aattcttcct
     7321 caatcttatc aatatatttc tatgattgtg acctgtgtag gtgtcagctt tgcttataat
     7381 ctttttgcag gcttgttgcg gtctattggt gacagtctag cagccctggg atttctgatt
     7441 ttctctgcct tggttaatgt ggttctggat ctctatttta ttacgcaatt gcatctggga
     7501 gttcaatccg caggacttgc taccattatt tcgcaaggtt tatcagcggt tctctgcttt
     7561 tattatattc gtaaaagtgt gccagaactc ttgccacagt ttaaacattt caaatgggac
     7621 aaaagcttgt acgcggatct cttggagcag ggtttggcta tgggcttgat gagttcaatt
     7681 gtatctatcg gcagtgtgat tttacagtct tctgttaata catttggtgc agtgattatt
     7741 agtgcccaga cggcagctcg acgcattatg acctttgccc ttcttcctat gaccgctatt
     7801 tctgcatcaa tgacgacctt tgcttctcag aatctaggag ctaagcgacc tgaccgcatt
     7861 gttcaaggtc ttcgaatcgg cagtcgttta agtatatcct gggcagtttt tgtttgtatt
     7921 ttcctctttt ttgccagtcc agccttggtt tccttcttgg ctagttcgac agatggttac
     7981 ttgatagaaa atggaagtct ctatctgcaa atcagttcag ccttttatcc cattttgagc
     8041 ctcttgttga tttatcgcaa ttgcttgcag ggcttggggc agaagatcct tcctctagtt
     8101 tctagcttta ttgaactaat cggaaaaatc gtttttgtgg ttttgattat tccttgggca
     8161 ggatataagg gtgttatcct ttgtgaacct cttatctggg ttgccatgac agttcaactg
     8221 tacttctcac tattccgtca tcccttgata aaagaaggaa aggcaatttt ggcaaccaaa
     8281 gtgcaatcct agttggattt actgaataaa atccatttcc tctagtgaaa atcgaaaaaa
     8341 cttgtgttct cttctttagt ttggtgttga aaatagttta acagactttt gacttctttt
     8401 atatgatata ataaagtata gtatttatga aaaggacata tagagactgt aaaaatatac
     8461 ttttgaaaag ctttttagtc tggggtgtta ttgtagatag aatgcagacc ttgtcagtcc
     8521 tatttacagt gtcaaaatag tgcgttttga agttctatct acaagcctaa tcgtgactaa
     8581 gattgtcttc tttgtaaggt agaaataaag gagtttctgg ttctggattg taaaaaatga
     8641 gttgttttaa ttgataagga gtagaatatg gaaattaatg tgagtaaatt aagaacagat
     8701 ttgcctcaag tcggcgtgca accatatagg caagtacacg cacactcaac tgggaatccg
     8761 cattcaaccg tacagaatga agcggattat cactggcgga aagacccaga attaggtttt
     8821 ttctcgcaca ttgttgggaa cggttgcatc atgcaggtag gacctgttga taatggtgcc
     8881 tgggacgttg ggggcggttg gaatgctgag acctatgcag cggttgaact gattgaaagc
     8941 cattcaacca aagaagagtt catgacggac taccgccttt atatcgaact cttacgcaat
     9001 ctagcagatg aagcaggttt gccgaaaacg cttgatacag ggagtttagc tggaattaaa
     9061 acgcacgagt attgcacgaa taaccaacca aacaaccact cagaccacgt tgacccttat
     9121 ccatatcttg ctaaatgggg cattagccgt gagcagttta agcatgatat tgagaacggc
     9181 ttgacgattg aaacaggctg gcagaagaat gacactggct actggtacgt acattcagac
     9241 ggctcttatc caaaagacaa gtttgagaaa atcaatggca cttggtacta ctttgacagt
     9301 tcaggctata tgcttgcaga ccgctggagg aagcacacag acggcaactg gtactggttc
     9361 gacaactcag gcgaaatggc tacaggctgg aagaaaatcg ctgataagtg gtactatttc
     9421 aacg
//
LOCUS       DAVMMR010000065         9369 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMMR010000065 DAVMMR010000000
VERSION     DAVMMR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.065"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            168..854
                     /locus_tag="VYS74_001738"
     CDS             168..854
                     /locus_tag="VYS74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3789738.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..1787
                     /locus_tag="VYS74_001739"
     CDS             855..1787
                     /locus_tag="VYS74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3789739.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1798..2727
                     /locus_tag="VYS74_001740"
     CDS             1798..2727
                     /locus_tag="VYS74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3789740.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2741..4180
                     /locus_tag="VYS74_001741"
     CDS             2741..4180
                     /locus_tag="VYS74_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789741.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4249..5826
                     /locus_tag="VYS74_001742"
     CDS             4249..5826
                     /locus_tag="VYS74_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3789742.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5886..7346
                     /locus_tag="VYS74_001743"
     CDS             5886..7346
                     /locus_tag="VYS74_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV3789743.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7368..7823
                     /locus_tag="VYS74_001744"
     CDS             7368..7823
                     /locus_tag="VYS74_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV3789744.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            8328..8777
                     /locus_tag="VYS74_001745"
     CDS             8328..8777
                     /locus_tag="VYS74_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3789745.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGLLVEEPINIKKVVFKVKI"
     gene            complement(8822..9369)
                     /locus_tag="VYS74_001746"
                     /pseudo
     CDS             complement(8822..9369)
                     /locus_tag="VYS74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         3220 a         1269 c         1749 g         3131 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa agccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgata tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gactttttat
     1141 tctctttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat taacagttta ctttcatcac
     1321 ttggaatgga gccaataagt ttctttacaa aatctgaatg gttcttgcct ctctacattg
     1381 gttcaggagt atggaaaaca ataggttggg gttctatcat ttatatggga gcattaacca
     1441 atatagattc aactttatat gaagcagcag atatggacgg ggctaataga tggcacaaaa
     1501 tgtggcatgt aactctacca agtttaagac ctacagtagt tactttgttt atactaaatg
     1561 ctggtggtat catgcaagta ggatttgaaa aggtattctt gatgtctagt cctgcaacat
     1621 atgaagtatc tgatgtatta tctacttatg tttatagaca aggtatttta aattctgatt
     1681 tcagttactc agctgcagta ggattattta ataacatagt agctttgtta tttgttttat
     1741 tagctaataa gattgctaaa aaacttggcg aagaaggaat tgtataaggg gggtaaaatg
     1801 attttttcaa aaaatacaaa aaagagtaat agagacaaag aaaccttcgt tgattatctc
     1861 tttatattca ttttaataat aataacctta cttatgttgt atccattatg gtgggtagtg
     1921 atgacgtcac tttcagaacc agtttggcta tctaaaaacg ttgttaaatt atttccctta
     1981 aaaatagata ttactgcata taagtttatt tttggggatt ctaaactatg gcaagcatat
     2041 ggtaatagtg tattgtatgc tgcactatct acaattgtta gtgttgcggt atgtagtatg
     2101 tatgcctatc cacttacaat tcctgaaatt aggggtaaaa aattctggaa tactcttatt
     2161 ttgattccaa tgttctttag tggaggaatg atcccgcttt atatacaagt tgcaaacctc
     2221 ggtttattga atactgcttg ggctcttgtt ttacctggtg ctgtttctac atattatata
     2281 attttgttta gaacacagtt taaatcaatc ccaatggagc ttcgtgagtc tgctagaatt
     2341 gatggggcaa gtgaatttag aattttttgg aatttagtat taccattatc aaaagttatt
     2401 ttcatgatta tagcacttta ttgcatagtt ggttcatgga atagttttac tcctccatta
     2461 ctttatttga atgacgacac taagatgcct ttatctattt atttgagaaa tttagttgtt
     2521 acaaatacaa ctgatactgc tgaattatca ctttctgcag gtaatacata ttaccaacaa
     2581 attgctgctc aaggtggagg tattggtatg cgtattgcta ttaagatggg tactattgtt
     2641 gttagtgtta ttccaattat gatgatttat ccatttattc agaaacattt tgttaaggga
     2701 atcctgctag gttctgtgaa aggttaataa ggtaattatt ttgcaaaatt taaaaaaata
     2761 tcttttatta gatagcggtt tagtagttgg gtatacatta gaaaatgctc aattaaaatt
     2821 aaataagcta aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga
     2881 aaatcctaaa ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa
     2941 agaattgaaa ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga
     3001 gactccttta gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc
     3061 actttgctat gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt
     3121 aaaatttaaa ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat
     3181 catgctagat gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga
     3241 attttctccg aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga
     3301 cgaacctatt ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag
     3361 agatccaaaa actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct
     3421 aagtgcaatt actttcagtg atgattttat taattggtca gaacctaaag aaatccttag
     3481 gggagatgga tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta
     3541 tttaggtttt gcttcaatgt accacgaagg cgacacttta aatgaaaact atgataaagt
     3601 agacttagaa ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga
     3661 ctatctcatt gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg
     3721 tattttcacg gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg
     3781 aaatggtaat cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga
     3841 ccaatttgct tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag
     3901 cttccaaact tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat
     3961 taaagttgcg gtaggtacta aatacggtaa tgcttattca gattttggtc aggaagatat
     4021 ctctcttgtt caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt
     4081 agatttaggt aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg
     4141 tattgaaggt agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa
     4201 ataatctcta tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt
     4261 aataaggtat taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt
     4321 ggcaataacg ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg
     4381 tttctatcta catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt
     4441 caatggttag aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga
     4501 gtaagttcta aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca
     4561 acaggagata agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta
     4621 gatttgtcac aacacatgga caaactacca aactataaga agtaccttga aaaaaataat
     4681 gcattagctc aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact
     4741 gatgagaata tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa
     4801 tttgaagatc tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg
     4861 actgaaaaac aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag
     4921 cgagttacac cattgtggaa tttaagccat cgtacatact atgactatga atcagattca
     4981 tataaacatc cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta
     5041 cgtaaagata acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt
     5101 ctattggctt ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat
     5161 aactttgata aatcatttga ttggcagcca cttgtttatc cagaaattaa tggaaaacgt
     5221 tatttgcaac cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca
     5281 ccaaaagaag aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat
     5341 caagaaaaac ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact
     5401 caagcaggta ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca
     5461 gaactgctgt ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt
     5521 gaggcacagc caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa
     5581 gatttaggtg gttttagacc agatactccg gttctttcct ttacaactga agaattagat
     5641 gttttaacta aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc
     5701 attgaaggac aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa
     5761 gacttggata aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg
     5821 aaatagacta aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt
     5881 aagctttgta tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg
     5941 cttcggggcg tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag
     6001 ttcctattaa tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag
     6061 taaatgcaag taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg
     6121 tctttaactc tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct
     6181 atcaaccaga agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata
     6241 ttttactaaa tgttgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg
     6301 aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag
     6361 ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg
     6421 gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa
     6481 caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc
     6541 gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta
     6601 tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat
     6661 gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat gaattaaaaa
     6721 aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa tctggagtca
     6781 ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca ttaattaatg
     6841 ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat gctaatattc
     6901 tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta ccaggttttg
     6961 aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt aggcacattg
     7021 ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt aagactatgt
     7081 atagctatca aactcctaaa gaaataacgt ataaatcaat tgtaggtgga aaattgaata
     7141 atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca caaattagtt
     7201 gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa gataaaagca
     7261 atactctaaa cattaataga gatttgttta aatcgaactt agatatatta tttacatggg
     7321 agttttctaa ttggattact gattaacaaa taataaggga atattaaatg aatgatacta
     7381 ataaaaatca atactttgtt tatgaatttg atactagctt tgtagaaatg tcttattctc
     7441 agttttttga aggatattgt attctttttt caaaagaacc ctatccttct ttatctgata
     7501 tgccaatgga atttagaaaa gaatttttaa tggaaatgag tatattaggt gatgcgatgc
     7561 ttgaagtgtt aaatgcctat agaattaatt atgatatctt aggaaattca acacctgtac
     7621 ttcatgctca tttatttccg agatacatgt gggaagagga agagagaaga aaaactgtag
     7681 ttcatagata tgattcttca gtttttagca atcctgagtt tgaatttact atagaaaagc
     7741 atggaccaat tattaaaaaa ctgaggtctg ctattgatag acattataga gagtatcaac
     7801 aattattaga tgattcaaac tgaactccac attttcgatt agatttaata ccttgtatat
     7861 tacaactagg ttgtggatga tataattttt cgaaaaatat attttaggaa tgacttataa
     7921 agtaattata tcagaggtta ttaaatcaat ggtccaagct tatgctcctt attcttattt
     7981 taaggttgga gctgcgttag aaactgaaga taaaaatttt tttctggatg taatatagaa
     8041 aatgttgctt ttctcctact tgctgtgcag aacgagtata tttttacaaa gctgtattta
     8101 aaggagaacg ttcgttttca aaaatatgta ttgtaggcgg aaaaaaaggt attttatcat
     8161 cttttatagc atcttgcagg gtgtcttgtc aggtaatgat agaattttgt aatcttgatt
     8221 catttgtagt tatattggct agtgatgttg atacttataa agtatataaa cttaaagaac
     8281 tactaccagt tagttttaat caagaaaatt tataacagga gggaactatg gaattagttg
     8341 aatttaaaaa tggacatgtt aatacggttc atgagcatat cgcaataacg aaagtaaaag
     8401 agtttctaaa taatcatgat ataccaaaag ataaaggtgg catacataaa atttctactg
     8461 atttctttta caatataatt gatatggaaa ctacaactga agaaaataga ccgtgggaaa
     8521 gtcataagga atattatgat gtccattatc tgttaaatgg agaagaaata attttatata
     8581 atttcttgtc tcaaatggaa ttgtctgaat ataaagtaga tgatgattgg caacaaatga
     8641 atgggacagc tttatttagt attaaattaa agaaagatat gttacttttg ttggaaccaa
     8701 acgatgcaca taaaacaggc ctattggttg aagaaccaat taatattaag aaagttgttt
     8761 ttaaggtaaa aatttaactg aaagtacttt cagggattaa gaattttaaa atatttaata
     8821 ctcatctgtt ttcaaaaagc attctagtcc atctccgatt aacgatggac tttatcacct
     8881 ctttctccag tccttgtatg acatcttgaa gttgattcat gacatcttcc aatatgggca
     8941 tcaacagctc cataacaata gcgaaattct cgtatatagt gactatggac atgtggacct
     9001 actcctattg gagaccaaca agatcccagt ttactgattc tactgaaacc agcctcatct
     9061 tggtacatca agcgaacctt acgaaaacgt ctactgattt taaagcgctt tcttgtcttc
     9121 ttgaattgag attttatttt tagacgcgac aatggtttga gcgtctgctt tcttaggatg
     9181 ttctggacgt ggcataatat ttcgccaacc atggcgcttc aacagttgat agaaggcatc
     9241 acgtgtgtag gaacgaccta actccttttt ataagcctga aataaggcat caattgtaac
     9301 aaattctcct gcctctgcag ccttcaaatg gcgggcaaga aagacttttt cttcctcaac
     9361 tgtcatata
//
LOCUS       DAVMMR010000066         9348 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMMR010000066 DAVMMR010000000
VERSION     DAVMMR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.066"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            155..1174
                     /gene="galE"
                     /locus_tag="VYS74_001747"
     CDS             155..1174
                     /gene="galE"
                     /locus_tag="VYS74_001747"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3789746.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1176..2141
                     /locus_tag="VYS74_001748"
     CDS             1176..2141
                     /locus_tag="VYS74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3789747.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFSAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(2192..2398)
                     /locus_tag="VYS74_001749"
     CDS             complement(2192..2398)
                     /locus_tag="VYS74_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV3789748.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2450..2926
                     /locus_tag="VYS74_001750"
     CDS             2450..2926
                     /locus_tag="VYS74_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV3789749.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2935..3606
                     /locus_tag="VYS74_001751"
     CDS             2935..3606
                     /locus_tag="VYS74_001751"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV3789750.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3736..4074
                     /locus_tag="VYS74_001752"
     CDS             3736..4074
                     /locus_tag="VYS74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV3789751.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4077..4706
                     /locus_tag="VYS74_001753"
     CDS             4077..4706
                     /locus_tag="VYS74_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV3789752.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4946..6097
                     /locus_tag="VYS74_001754"
     CDS             4946..6097
                     /locus_tag="VYS74_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3789753.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6410..7159
                     /gene="truA"
                     /locus_tag="VYS74_001755"
     CDS             6410..7159
                     /gene="truA"
                     /locus_tag="VYS74_001755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV3789754.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7149..7922
                     /locus_tag="VYS74_001756"
     CDS             7149..7922
                     /locus_tag="VYS74_001756"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3789755.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7900..8361
                     /locus_tag="VYS74_001757"
     CDS             7900..8361
                     /locus_tag="VYS74_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3789756.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            8588..9348
                     /locus_tag="VYS74_001758"
                     /pseudo
     CDS             8588..9348
                     /locus_tag="VYS74_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2725 a         1719 c         2081 g         2823 t
ORIGIN      
        1 ccatggcaag gcgacgttga cgtggtttga attggatttt cgaagagtat aagcatttta
       61 ctgaaatttt atcaggtagt ttaggatggc aaatgacatt ccctgtcaaa aatggtaaaa
      121 taagaaaaaa tgatctgaga atcgaggaaa aaagatgcaa gaaaagattt tggtaactgg
      181 tggtgccggt tttattggaa cccacactgt tattgaattg atccaagcag gccatcaagt
      241 tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt ttagaagttg ttgaaagaat
      301 cacaggagtt gagattcctt tctatgaggc agatattcgt gacacagata ccctcagaga
      361 tattttcaag caggaagaac cgactggtgt cattcacttt gctggtttga aggctgttgg
      421 cgaatcaaca cgtatccctc ttgcctacta tgacaacaat atcgctggaa ctgtcagcct
      481 tttgaaagcc atggaagaaa acaactgtaa aaacatcatc ttcagttctt ctgcgacagt
      541 ttacggggat ccgcacacag tgcccatctt ggaagatttc ccactttcag tgacaaaccc
      601 atacggtcgt accaagctca tgctagagga aattttgacc gatatttaca aagcagactc
      661 agaatggaat gttgtcttgc ttcgttactt taacccaatc ggagcccatg agagtggtga
      721 tttgggagaa aatccaaacg gtattccaaa caatctcttg ccatatgtga ctcaagtagc
      781 cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat tacgatacgg aagatggaac
      841 aggtgttcgt gactatatcc acgttgtcga tttggctaag ggtcacgttg cagctttgaa
      901 aaaaatccaa aaaggttcag gactaaacgt ttataacctt ggaactggta aaggttactc
      961 agttcttgaa attatccaaa acatggaaaa agcggtggga cgtcctattc cttaccgcat
     1021 cgtagaacgt cgcccaggtg atatcgctgc ctgctactca gacccagcaa aagctaaagc
     1081 agaactcggt tgggaagcag aactcgacat cacccaaatg tgtgaagacg catggcgttg
     1141 gcaaagcaag catccaaatg gatttgaaga ctaagatgat gatttcaatc atcgtccctt
     1201 gtttaaacga agaggaagta cttcctcttt tttatcaggc tctggaagct ttacttccag
     1261 atttggaaac agaaatcgag tatgtctttg tcgatgatgg atcaagtgat gggaccttgg
     1321 aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca ttatatttct ttctctcgaa
     1381 attttggcaa agaagcagcc ctttatgcag gcttgcaata tgcgacagga gatttggtgg
     1441 tggtgatgga tgcagacctc caagatcctc ctagtatgtt gtttgagatg aaaaatgtac
     1501 tagacaaaaa tgtagacttg gactgcgttg ggacacggag aactagtcgg gagggagaac
     1561 ccttctttcg cagtttctct gctgttctct tttatcgcct catgcaaaaa atcagcccag
     1621 tagctctgcc gtcgggtgtc cgtgattttc gtatgatgag aaggtctgta gtcgatgcca
     1681 ttttaagctt gaccgagtcc aatcgttttt caaaggggct cttcgcctgg gtgggtttca
     1741 aaacctacta tttggactat ccaaatgtcg aaaggcaggc tggcaagaca agttggagtt
     1801 ttaggcaact ctttttctac tccattgaag ggattattaa tttttcagat tttcccttga
     1861 ttatagcctt tgtagctggt ctcctatctt gttttctttc tctgctgatg accttttttg
     1921 ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg ttggacctct ctgatggctg
     1981 ttattctctt tcttggaggc attcaactct tgaccattgg gattctcggc aagtatatca
     2041 gtaagattta tttagagact aaaaaaagac cactttatct tatcaaagaa aaaagtgacc
     2101 ttcctgattt tacagaaaaa aataaagtga aaagactata attttacatg gaaatgtgct
     2161 aaactagagg gagtggatta tcgccattga tttaggcttc ctggtttcca atcagggcgc
     2221 gagttgggca atttttaaca gcctctaaga catcctgact aggagaaatt tctttttcca
     2281 gttggtcagg gtcatcgtaa aaacgcacga ttccattatc gtggtaatca aataaatcag
     2341 aataagtttg gcaaagccca caggcgatac atcgttcagg tataagtgtg attttcatgt
     2401 tcatatttat attgtaataa gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga
     2461 accgtggcaa gaagatatct atgatcaaga agaatcaaga gcagagcgtc ggcatcgaaa
     2521 ccacggaggg gcggatagga tggctaatcg tattttgacg atcctagcta gtattttctt
     2581 tgtaattgtg gtggtgatgg tcatcgttct catctatcta tcatcggggg ggagtaatcg
     2641 cacagcagcc ttaaaagact ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc
     2701 aagcagttct cagcctgagc agagttcaga gccagaatct acttctagta gttcagaaga
     2761 agctgctaat cctgaaggaa cgattaaagt tctcgcagga gaaggggaag cagctattgc
     2821 cgctcgtgca ggaatctcca ttgctcagtt agaggccttg aatcctgggc acatggctac
     2881 aggatcttgg tttgctaatc caggtgatgt tataaaaata aaataggagc cgatatgaaa
     2941 acaattcaaa ttgctattga tggtcctgct tccagcggta agagtacggt cgcaaagatt
     3001 attgctaagg attttggatt cacctacctt gatacaggag ctatgtatcg tgcagcgacc
     3061 tatatggctc ttaagaacca attaggagtt gaagaagtcg aagcccttct agccttgttg
     3121 gaccagcatc caatcagctt tggacgttca gaaactggag accagcttgt ttttgtagga
     3181 gatgtggata ttacccatcc tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt
     3241 gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc aacaagaaat tgcccagcaa
     3301 ggcgggattg ttatggatgg tcgcgatatt ggaactgttg tattgccgca agcagaattg
     3361 aaaattttcc tagtagcttc tgttgatgag agagcagagc gtcgttacaa ggaaaatatt
     3421 gccaagggaa ttgaaacaga ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac
     3481 aaggatagtc atcgtgagac ttctcctctc aaacaagcag aggatgctgt ctaccttgat
     3541 acaactggtt tgaacattca agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga
     3601 atgtaaatgt agaaaagccg atagattgat atcggttttt tctaaatttg tcaaaatgct
     3661 gattttaggg tataatagtt gctgttcgag aaattttgat tttcaaagaa tagtgaatga
     3721 tgataaggag aaatcatgaa caatttacca aattgtccaa aatgtaactc agagtatgtc
     3781 tacgaagacg gtgccctact ggtttgccca gagtgtgctc atgagtggaa tcctgctgaa
     3841 gttgcagaag tagaagaagg tcttgtcgct atcgatgcca acggaaataa attgactgat
     3901 ggtgatacag taactctcat caaggacttg aaagtaaaag gtgcgccaaa agatttgaaa
     3961 caagggacgc gcgtgaaaaa tatccgcatc gtagaaggcg atcacaatat cgactgtaag
     4021 attgatggtt ttggtgccat gaaacttaaa tcagagtttg tgaggaagat ttaagcatgt
     4081 caaaacacta taaacttgta ttttatagcc gtatcttctt gtttctagcg gcttttacgg
     4141 gagtttatct tgaaattact aggcatggtg gttttgggat gcttctctat tacacggttc
     4201 tgtccaacct cttggtaact atttttaccc tttatcttct aaaggttatg agccgtgtag
     4261 gtgaaaattg gcaaaggcca agtctcttgc gcttaaaagg tggggtcacc atgagtatca
     4321 tgattacctg tgtgatttac catttcctct tggcgcccat tgcgactaat ttctataccc
     4381 tagaaaattt cctttgccac tatatcgttc ccatctggtt tttagcggat accctctttt
     4441 ttgacaaaca gggtcaatac aagatttggg atccagcagt gtggaccatt ttaccctttc
     4501 tgtatatgat gtttgctctt tttaatggct tggttctaaa actcaatatt ccaaatgcca
     4561 aggataatcc tttcccttac ttctttttga atgtgaacaa gggttggaat gttgtgttta
     4621 agtggtgtct gattatcttt gttgcctata tggtagcagg atttattttc tactttatca
     4681 agcaaatcaa gagaaagtca tcctaatact cttcgaaaat ctcttcaaac cacgttagtt
     4741 tccatctgca acctcaaaac actgttttga acaacctgcg gctagctttc tagtttgctc
     4801 tttgattttc attgagtatg agatacaagg gattcatttt cgaatttaag aaggagtcta
     4861 caaaccaatg aggcttcttc ttttcttgct tatttgataa ctatcaaaaa agtagaagat
     4921 taagagaaat atagaaagag atttcatgaa acaattttta gaacgggcca gcattttggc
     4981 tctctccctc gttttgatta cctccttttc catttcgagt gccctaccag ccatgtttga
     5041 ctattatcag ggttattcta aggaacaaat tgagctcttg gtgagcttgc cttcctttgg
     5101 aatcatgatg atgttactgc taaatggttt cttagaaaaa atatttcctg agcgcttaca
     5161 gattagtttg ggcttgctga ttttatcatt gagcggtaca gctcccttct ggtaccaagc
     5221 ctatcccttt gtctttggaa cacggcttct ctttggtttg ggtcttggga tgatcaatgc
     5281 caaggccatt tctattatca gtgaacgcta ccaaggaaaa aggcgaattc agatgttagg
     5341 gctacgcgct tctgcagagg tcgttggagc ttctctcatt accttggccg tcggtcagtt
     5401 gttggccttt ggttggacag ctatctttct agcctatagt gctggatttt tggtgatgcc
     5461 cctttatctg ctctttgtcc cttatggaaa atcaaagaaa gaagtcaaga aaagagcgaa
     5521 ggaagcaagt cgtttaactc gagaaatgaa aggcttgatt tttaccttag ctatcgaagc
     5581 ggcagttgta gtttgtacca atacagctat taccatccgt attccaagtt tgatggtgga
     5641 aagaggattg ggggatgccc agttatctag ttttgttctt agtatcatgc agttgatcgg
     5701 gattgtggct ggggtgagtt tttctttctt gatttctatc tttaaagaga aactgctcct
     5761 ctggtctggt attacctttg gcttggggca aatcgtgatt gccttgtctt catccttgtg
     5821 ggtggtagta gcaggaagtg ttctggctgg atttgcctat agtgtagtct tgacgacggt
     5881 ctttcaactt gtctctgaac gaattccagc taaactcctc aatcaagcaa cttcatttgc
     5941 tgtattaggc tgtagtttcg gagcctttac gaccccattt gttctaggtg caattggctt
     6001 actaactcac aatgggatgt tggtctttag tatcttagga ggttggttga ttgtaatctc
     6061 tatctttgtc atgtacctac ttcagaagag agcttaggat tgattcctaa gtttttcttt
     6121 tgatactcaa tgaaaatcaa agagcatagt tgattgagtt tggaatagta cgctgtagct
     6181 gctaaaatat ttctagaaat taatttgact tttctaatag agttattcat atcttatttc
     6241 aatctactat agaaagctag ccgcaggttg ctcaaaatag tgttttgagg ttgcagatgg
     6301 aaactgacgt ggtttgaaga gattttcgaa gagtatgaga tttttatacc catacaatat
     6361 ttgttttagg tccttgttta cagactaatt tcttgataaa atagaggtta tgacgagata
     6421 taaagcaact atttcctatg atggttatgc ctttgctggc tttcagcgcc agtctcatgc
     6481 gcgtagcgtt caggaagaaa ttgaaaaaac cttgaccaga ttaaataaag ggcaaaccat
     6541 tactgttcac ggtgctggta ggacagatag tggggttcat gccctgggac aggtcattca
     6601 ttttgacctg ccttatcaga tggatgagga gaaactccgt tttgccttgg atacccagtc
     6661 tcctgaagat attgatgtga tttcgattga gcttgtggca gatgattttc attgccgtta
     6721 tgccaaacat agcaagacct atgagtttat tgtggataga ggacgtccca aaaatcctat
     6781 gcgccgtcac tatgccaccc actttcccta cccactcgat gtggaacgaa tgcagattgc
     6841 aatcaaaaag ctagagggaa cccatgattt taccggtttt acagcctctg ggactagtgt
     6901 agaggataag gttcgcacca tcacagaagc tagtttaata gtcgatgaga caggacaatt
     6961 tttgaccttt accttttcag gaaatggttt cttgtataaa cagattcgca atatggtggg
     7021 gactcttctc aaaatcggta acaaccgcat gccagtagag cagattgatc tcatcttgga
     7081 gaagaaggac aggcaacttg caggtcccac tgcagcacca aatggtttgt atttaaagga
     7141 gattcgttat gaagaataat cgtattttag cactttctgg aaatgatatt tttagtggtg
     7201 gtggactgtc agctgatttg gctacctata ccttgaacgg cttgcatggg tttgtagcag
     7261 tgacttgttt gacagccttg acagaaaaag gatttgaagt ctttccaact gatgatacca
     7321 tttttcaaca tgaattagat agcttacgtg atgtggaatt tgggggaatt aagattggtc
     7381 ttctccctac tgtcagtgtg gctgagaagg ccttggactt tatcaaacaa cgcccaggag
     7441 tacctgtggt gttggatcct gtcttggtct gcaaggaaac gcatgatgta gctgtcagtg
     7501 agctctgcca agagttgatt cgctttttcc cttatgtcag tgtgattacg cctaatctcc
     7561 cagaagcaga attattatcc ggtcaggaaa ttaaaacctt ggaagacatg aaaactgcag
     7621 cgcagaaatt gcatgattta ggagcgccag cagtcattat caagggaggc aatcgtctta
     7681 gtcaggacaa ggctgtggat gtcttttatg atggacagac ctttactatc ctagaaaatc
     7741 cagttatcca aggccaaaat gctggtgcag gttgtacctt tgcctctagc attgccagtc
     7801 acttgattaa aggtgataaa cttttgccag cagtagaaag ctctaaggct ttcgtttatc
     7861 gtgctattgc acaagcagat cagtatggag taagacaata tgaagcaaac aaaaacaact
     7921 aaaatcgccc ttgtatccct attaaccgcc ctttctgtgg ttctaggtta tttcttaaaa
     7981 atcccaacac ctacaggaat tctaactctt ttagatgctg gtgtcttctt tgcggccttt
     8041 tactttggta gtcgtgaagg agcggtagtc ggaggactag caagtttctt gattgacctc
     8101 ttatcaggct accctcagtg gatgttcttt agcttggtca accatggctt gcagggattt
     8161 ttcgcaggat ttaaaggaaa aagtcagtgg ttaggcctta ttttagcaac tattgccatg
     8221 gtaggaggct acgccttggg ttctactttg atgaatggct gggcagcagc cctcccagaa
     8281 attctaccaa atttcatgca aaatatggta gggatgattg taggatttat tcttagtcaa
     8341 agtatcaaga agattaagta atactcttcg aaaatctctt caaaccacgt cagcttccat
     8401 ctgcaacctc aaaacactgt tttgagcagc ctgaggctag ctttctagtt tgcactttga
     8461 ttttcattga gtataaagag gttgagtaaa aagtatttca aataagaaaa acgcatagta
     8521 tcaggtgttg aatgaactgc cccccaaaag ttagacagaa aaaatctaac ttttggggtg
     8581 ttttattatg aaactaactt atgatgataa agttcagatc tatgaactta gaaaacaagg
     8641 atatagctta gagaagcttt caaataaatt tgggataaac aattctaatc ttaggtacat
     8701 gattaaattg attgatcgtt acggaataga gttcgtcaaa aaagggaaaa atcgttacta
     8761 ttctcctgat ttaaaacaag aaatgattca taaagtctga catgaaggct agactaaaga
     8821 tagagtttct cttgaatacg gtctcccaag tcgtacgata cttcttaact ggctagcaca
     8881 atacaggaaa aacgggtata ctattgttga gaaaacaaga gggagagtac ctaaaatggg
     8941 acgtaagcca aaaaagagac ctgaagagag gacagaatta gaacgtcttc aagcagaaaa
     9001 tgagtacctg agagcggaga atgccatcct aaaaaagtta agagaactcc gattgaagga
     9061 ggaaaaagag aaagacagaa attgtttaag aattaatgac tgagttttcg ttagatcttc
     9121 ttctaaaagc cattaaacta gctcgttcga cctactacta tcacttgaaa cagctagaca
     9181 aaacagataa ggaccaagag cttaaagctg aaattcaatc catttttatc gaacacaagg
     9241 gaaattatgc ttatcgtcga attcatttag aactaagaaa tcgtggttat ctggtaaatc
     9301 ataaaagagt tcaaggcttg atgaaagtac tcaatttaca agctaaaa
//
LOCUS       DAVMMR010000067         9288 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMMR010000067 DAVMMR010000000
VERSION     DAVMMR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.067"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            166..2616
                     /locus_tag="VYS74_001759"
     CDS             166..2616
                     /locus_tag="VYS74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV3789757.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVENGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQESLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            2653..3876
                     /locus_tag="VYS74_001760"
     CDS             2653..3876
                     /locus_tag="VYS74_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3789758.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            3887..4441
                     /locus_tag="VYS74_001761"
     CDS             3887..4441
                     /locus_tag="VYS74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV3789759.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            4526..5089
                     /locus_tag="VYS74_001762"
     CDS             4526..5089
                     /locus_tag="VYS74_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3789760.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            5104..7050
                     /gene="gyrB"
                     /locus_tag="VYS74_001763"
     CDS             5104..7050
                     /gene="gyrB"
                     /locus_tag="VYS74_001763"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV3789761.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            7132..8859
                     /gene="ezrA"
                     /locus_tag="VYS74_001764"
     CDS             7132..8859
                     /gene="ezrA"
                     /locus_tag="VYS74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV3789762.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEITSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
BASE COUNT         2823 a         1683 c         1978 g         2804 t
ORIGIN      
        1 catttcttct ctcaattttt cgtatttctt cactatttta caaaaatcag caggctagaa
       61 tgctctattc gatgggattt ttaagaaaag tagtgttctt gagtttgaaa attatcctat
      121 gtttgcaggt gccaaatggc cctttttttg gtataatttt ttataatgaa aacgattggt
      181 aatcgctatg ttgtggtgga tttagaggca actagcacag gtagtaaggc taaaattatc
      241 caagtgggaa ttgtcgtgat tgaggacgga gaaatcgtcg atcactatac gacggatgtc
      301 aatccacatg aacccttgga tgctcatatc aaagaactga caggattgac agaccaacgt
      361 ctggcgcaag cacctgattt ttcgcaagtt gccagaaaaa tatttgactt ggtggagaat
      421 gggatttttg tagcccataa tgttcagttt gatgctaatc tcttggcgga aaatttattt
      481 tttgaaggct atgagctaag aaaccctcgt gttgatacgg tcgaattggc ccaggtcttt
      541 ttccctgaac tggaaaaata tagcttgccg attttgtgtc gagaattagg aattcctctt
      601 aaacacgcac acacagccct ttcagatgcc caagctacag cagaattact tcttttttta
      661 cgggaaaaga tgacccagct tcctaaaggt ctcttggaac gcttgctgga aatggctgac
      721 gctctcctat atgagtccta cctggttatt gaggaaactt atcgcaacca atctatcctg
      781 agttctccag acttggtcca agttcaaggt ctgtatttta agaaaacggc agcttctctg
      841 gagctacgaa aactatctca agacttttct aaaaatattt ctctgttgaa ccttgaagtg
      901 agggaggaac aagaaagttt tgctaaagag gttggcttgc tattgaaaga tgaacctgtc
      961 tctctgattc aagcgccgac agggattggg aaaacctatg gctatctctt acccgcttta
     1021 tctcaatcca aagagcgaca aattgttctt agtgttccga caaagattct tcaaaatcaa
     1081 atcatggaag aagaaggtaa acgtctcaag gaagtgttcc atacagatat tcatagctta
     1141 aagggaccac aaaattatct gaagttggat gccttttatc attccttgca ggaaaatgat
     1201 gaaaatcgct tatttagacg ctttaaaatg caagtcttgg tctggcttac tgagacagag
     1261 acaggagatt tggatgaaat cgggcaactc taccgttacc aacattttct agcagacctt
     1321 cgtcatgatg ggaatttatc atcccagagc ttatttgtga cggaagattt ttggaaacgt
     1381 agtcaagaaa gggcagagac ttgcaagctt ttagtgacta atcatgccta tctcgtgacc
     1441 agacttgaag ataatcctga atttgtcagt gaccgtttac tgattattga tgaagtccaa
     1501 aagattttgt tagctctaga aaatctgctt caagagacct acgatataca atctattatc
     1561 gatttaattg ataaggcttt agtaggagaa gaaaacaggg ttcaacaacg gatactagaa
     1621 agtattcgct ttgagtgtct ctacttgata gaacaatttc agtctggcaa atctaggaaa
     1681 aatatcttag attctctgga caatctccat cagtattttt cagaattaga agtggaaggc
     1741 tttgatgagc tggttcgcta ttttacagct gaaggtgatt actggcttga agtaactgaa
     1801 acgagtcaaa agaaaattca gatttcttct acaaaatcag gccgtactct tctgtcctct
     1861 ttacttcctg agagttgcca agtcctggga gtatcggcta ctcttgagat tagtcagagg
     1921 gtttctttgg cagacctttt aggctatcct gaagccaaat ttgtcaagat tgaatctcgg
     1981 ggaaaacagg aacaagaagt ggttatggtc aaagatttcc ctctggtaac agaaacctcc
     2041 ttagaagtct atgccagaga ggtagctgct ttactagtgg aaattcaagc tttccagcaa
     2101 ccgattttgg ttctctttac cgctaaagac atgcttctag cagtatcgga tttacttaca
     2161 gttagccact tggcccagta taaaaatggg gatgttcatc agctaaagaa acgctttgaa
     2221 aaaggtgaac aacaaatctt gcttggtgca gcaagtttct gggagggagt tgatttttca
     2281 agccatcctt ttgtgattca agttgtaccg agacttcctt tccaaaatcc tcaagaatcc
     2341 ttgacgaaaa agattaatca agaactgaat caagaaggga aaaatgcctt ttatgattat
     2401 caattgccaa tggccattat tcgtttaaaa caggctttgg gaagaagtat gagacgtgaa
     2461 taccaacgtt ccttaactct tattttggat aggagaatcg tcggaaaacg atacggtaaa
     2521 caaatagtag catctctagc agaagaagca actgttaaaa ccatctctcg atccgaagtt
     2581 gacgaggcta ttgatagatt ttttaatgaa ctttgataaa tagtattgta tgaaagtata
     2641 aggttagtat atatgaaacg ttctctcgac tctagagtcg attatagttt gctcttgcca
     2701 gtattttttc tactggtcat cggtgtggtg gctatctata tagccgttag tcatgattat
     2761 cccaataata ttctgcccat tttagggcag caggtcgcct ggattgcctt ggggcttgtg
     2821 attggttttg tggtcatgct ctttaataca gaatttcttt ggaaggtgac cccctttcta
     2881 tatattttag gcttgggact tatgatcttg ccgattgtat tttataatcc aagattagtt
     2941 gcatcaacgg gtgccaaaaa ctgggtatca ataaatggaa ttaccctatt ccaaccgtca
     3001 gaatttatga agatatctta tatcctcatg ttggctcgtg tcattgtcca atttacaaag
     3061 aaacataagg aatggagacg cacggttccg ctggactttt tgttaatttt ctggatgatt
     3121 ctctttacca ttccagtcct agttctttta gcacttcaaa gtgacttggg gacggctttg
     3181 gtttttgtag ccattttctc aggaatcgtt ttattatcag gggtttcttg gaaaattatt
     3241 atcccagtat ttgtgactgc tgtaacagga gttgctggtt tcttagctat ctttattagc
     3301 aaggacggac gagcttttct tcaccagatt ggaatgccga cctaccaaat caatcggatt
     3361 ttggcttggc tcaatccctt tgagtttgcc caaacaacga cttaccagca ggctcaaggg
     3421 cagattgcca ttgggagtgg tggcttattt ggtcagggat ttaatgcttc gaatctgctt
     3481 atcccagttc gagagtcaga tatgattttt acggttattg cagaagattt tggctttatt
     3541 ggctctgtcc tggttattgc cctctatctc atgttgattt accgtatgtt gaagattact
     3601 cttaaatcaa ataaccagtt ctacacttat atttccacag gtttgattat gatgttgctc
     3661 ttccacatct ttgagaatat cggtgctgtg actggactac ttcctttgac ggggattccc
     3721 ttgcctttca tttcgcaagg gggatcagct attatcagta atctgattgg tgttggtttg
     3781 cttttatcga tgagttacca gactaatcta gctgaagaaa agagcggaaa agtcccattc
     3841 aaacggaaaa aggttgtatt aaaacaaatt aaataaggag aaaatcatgg taaaagtagc
     3901 agttatgtta gctcagggct ttgaagaaat tgaagccttg acagttgtag atgtcttgcg
     3961 tcgagccaat atcacatgtg atatggttgg ttttgaagag caagtaacgg gttcgcatgc
     4021 aatccaagta agagcagatc atgtctttga tggaaattta tcagactatg atatgattgt
     4081 tcttcctgga ggtatgcctg gttctgcaca tttacgtgat aatcagacct tgattcaaga
     4141 attgcaaagc ttcgagcaag aagggaagaa actagcagcc atttgtgcgg caccaattgc
     4201 cctcaatcaa gcagagatat tgaaaaataa gcgatacact tgttatgacg gcgttcaaga
     4261 gcaaatcctt gatggtcact acgtcaagga aacagtagtg gtagatggtc agttgacaac
     4321 cagtcggggt ccttcaacag cccttgcctt tgcctacgag ttggtggagc aactaggagg
     4381 ggacgcagag agtttacgaa caggaatgct ctatcgagat gtctttggta aaaatcagta
     4441 aaacgggagt tattctctcg ttttttatgt ggaaaactca gggaaatcat cgcttttttc
     4501 ataaaaaaat gctataatga agggtatgaa atatcacgat tacatctggg atttaggtgg
     4561 aactttactg gataattatg aaacttcaac agctgcattt gttgaaacat tggcactgta
     4621 tggtatcaca caagaccatg acagtgtcta tcaagcttta aaggtttcta ctccttttgc
     4681 gattgagaca ttcgctccca atttagagaa ttttttagaa aagtacaagg aaaatgaagc
     4741 cagagagctt gaacacccga ttttatttga aggagtttct gacctattgg aagtcatttc
     4801 aaatcaaggt ggccgtcatt ttttggtctc tcatcgaaat gatcaggttt tggaaatttt
     4861 agaaaaaacc tctatagcag cttattttac agaagtggtg acttctagct caggctttaa
     4921 gagaaagcca aatcccgaat ccatgcttta tttaagagaa aagtatcaga ttagctctgg
     4981 tcttgtcatt ggtgatcggc cgattgatat cgaagcaggt caagctgcag gacttgatac
     5041 ccacttgttt accagtatcg tgaatttaag acaagtatta gacatataag aaaaaggaat
     5101 aagatgacag aagaaatcaa aaatctgcag gcacaggatt atgatgccag tcaaattcaa
     5161 gttttagagg gcttagaggc tgttcgtatg cgtccaggga tgtacattgg atcaacctca
     5221 aaagaaggtc ttcaccatct agtctgggaa attgttgata actcaattga cgaggccttg
     5281 gcaggatttg ccagccatat tcaagttttt attgagccag atgattcgat tactgttgtg
     5341 gatgatgggc gtggtatccc agtcgatatt caggaaaaaa caggccgtcc tgctgttgag
     5401 accgtcttta cagtccttca cgctggagga aagttcggcg gtggtggata caaggtttca
     5461 ggtggtcttc acggggtggg gtcgtcagta gttaatgccc tttccactca attagacgtt
     5521 catgttcaca aaaatggtaa gattcattac caagaatacc gtcgtggtca tgttgtcgca
     5581 gatcttgaaa tagttggaga tacggataaa acaggaacaa ctgttcactt cacaccggac
     5641 ccaaaaatct tcactgaaac aacaatcttt gattttgata aattaaataa acggattcaa
     5701 gagttggcct ttctaaatcg cggtcttcaa atttcaatta cagataagcg tgaaggtttg
     5761 gaacaaacca agcattatca ttatgaaggt gggattgcta gttacgttga atatatcaac
     5821 gagaataagg atgtaatctt tgatacacca atctatacag acggtgagat ggatgatatc
     5881 acagttgagg tagccatgca gtacacaact ggttaccatg aaaatgtcat gagtttcgcc
     5941 aataatattc atacacatga aggtggaacg catgaacaag gtttccgtac agccttgaca
     6001 cgtgttatca atgattatgc tcgtaaaaat aagttactga aagacaatga agacaaccta
     6061 acaggggaag atgttcgcga aggcttaact gcagttatct cagttaaaca cccaaatcca
     6121 cagtttgaag gacaaaccaa gaccaaattg ggaaatagcg aagtggtcaa gattaccaat
     6181 cgcctcttca gtgaagcctt ctccgatttc ctcatggaaa atccacagat tgccaaacgt
     6241 atcgtggaaa aagggatttt agctgccaag gctcgtgtgg ctgccaagcg tgcgcgtgaa
     6301 gtcacacgta aaaaatctgg tttggaaatt tccaaccttc cagggaaact agcagactgt
     6361 tcttctaata accctgctga aacagaactc ttcatcgtcg aaggagactc agctggtgga
     6421 tcagccaaat ctggtcgtaa ccgtgagttt caggctatcc ttccaattcg cggtaagatt
     6481 ttgaacgttg aaaaagcaag tatggataag attctagcta acgaagaaat tcgtagtctt
     6541 ttcacagcca tgggaacagg atttggcgca gaatttgatg tttcgaaagc ccgttaccaa
     6601 aaactcgttt tgatgaccga tgccgatgtc gatggagccc acattcgtac ccttctttta
     6661 accttgattt atcgttatat gaaaccaatc ctagaagctg gttatgttta tattgcccaa
     6721 ccaccaatct atggtgtcaa ggttggaagc gagattaaag aatatatcca gccgggtgca
     6781 gatcaagaaa tcaaactcca agaagcttta gcccgttata gtgaaggtcg taccaaaccg
     6841 actattcagc gttataaggg gctaggtgaa atggacgatc atcagctgtg ggaaacaacc
     6901 atggatcccg aacatcgctt gatggctaga gtttctgtag atgatgctgc agaagcagat
     6961 aaaatctttg atatgttgat gggggatcga gtagagcctc gtcgtgagtt tatcgaagaa
     7021 aatgctgtct atagtacact tgatgtctaa aaaataggga gaagtagttc ccttggtcta
     7081 aaaaatatga tataatcgtt acgattgttt caagtaaaaa aggagtttga tatgtctaat
     7141 ggacaactaa tttatttaat ggttgcaatt gcagtcattt tagttctggc ttatgtagtg
     7201 gcaatctttc tacgtaagcg aaacgagggg agattagagg cgctagaaga aagaaaagaa
     7261 gaactataca atcttccagt aaatgatgaa gtagaagctg taaaaaatat gcacttgatt
     7321 ggacaaagtc aagtggcttt ccgtgaatgg aatcaaaaat gggtcgattt atctctcaac
     7381 tcttttgccg atattgaaaa taatctcttt gaagcagaag gctataacca ttcatttcgt
     7441 tttctcaagg ccagtcatca aattgaccaa attgagagtc aaattacttt gattgaagaa
     7501 gatattgcgg caattcgcaa tgctttggca gacttagaga agcaagaatc taaaaatagt
     7561 ggtcgtgttc ttcatgcttt ggatttattt gaggaacttc agcatagagt tgctgaaaat
     7621 tcagaacagt atggtcaagc cttggatgaa attgaaaaac aattagaaaa tatccaatct
     7681 gaattttcac aatttgtaac cttgaattca tcgggtgacc ctgtggaagc cgcagtgatt
     7741 ttggataata cagaaaatca cattttggcc ttaagtcata ttgtggatcg tgttccagcc
     7801 ttggttacga cgctttctac agaattgcca gatcaattac aggatttgga agccggttat
     7861 cgtaaactaa ttgatgctaa ttatcatttt gttgaaacgg atattgaagc gcgtttccac
     7921 ttgctttatg aagcattcaa gaaaaaccaa gagaatattc gtcagttgga attggataat
     7981 gccgaatatg agaatggaca ggcacaagag gaaatcaatg ccttgtatga tatttttact
     8041 cgagaaattg ctgctcagaa agtagtggaa aatctacttg caactcttcc aacttacctt
     8101 caacatatga aagagaataa tactttattg ggagaagata ttgcacgttt gaacaagacc
     8161 tatttgcttc ctgagacagc tgcaagccat gttcgtcgta ttcagacaga attagagagt
     8221 tttgaggcag ctattgttga gataacttca aatcaagaag aaccaaccca agcttattca
     8281 gttcttgaag aaaatcttga ggatttacaa actcaactaa aagatattga agatgagcaa
     8341 atttcagtta gtgagcgcct gacacaaatt gagaaagatg atattaatgc acgtcaaaag
     8401 gccaatgttt atgtcaatcg tctccatact atcaagcgat acatggaaaa acgcaatctg
     8461 ccaggtattc cacaaacttt cttgaagtta ttctttacgg caagcaataa taccgaggat
     8521 ttaatggttg agttagaaca aaaaatgatt aacattgaat ctgttacccg agttcttgaa
     8581 attgcaacga atgatatgga agctttagaa acggaaactt ataatattgt acaatatgca
     8641 actttgacag agcaactctt gcaatattct aaccgctatc gctcatttga tgaacgcatt
     8701 caagaagcat ttaacgaagc tttagatatt tttgaaaaag aatttgatta tcacgcttca
     8761 tttgacaaga tttctcaagc attggaagtg gcagagcctg gtgtaaccaa tcgctttgtt
     8821 acctcatatg agaaaacacg tgaaacgatt cgtttttaat aaaagaaaaa gattttattg
     8881 tgtgaggagc agaatcaaat ctttttctat agttgtgggg agatttactt cattttctcc
     8941 tgagattgag tttttgccca gccgatttat ccaccacctc aaaacagtgt tttatactct
     9001 tcgaaaatct cttcaaatca cgtcagcgtc gccttaccgt actcaagtac agcctgaggc
     9061 tcttagtttg ctttttgatt ttcatttagt attaaagtga tttcgtcagt cttatctgca
     9121 gcttcaaatc tgtactttga gtaacttggt aactgtccaa taacgaagtc tattgaaaaa
     9181 tctccagact agagaactca cggatagttc ctaatctgga gatttcttat ttgcactttt
     9241 cttgtacaac tttagtccac ggtaaatagg cctctaaaac ctctttgt
//
LOCUS       DAVMMR010000068         8894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMMR010000068 DAVMMR010000000
VERSION     DAVMMR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.068"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(14..229)
                     /locus_tag="VYS74_001765"
     CDS             complement(14..229)
                     /locus_tag="VYS74_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789763.1"
                     /translation="MGKERREQEHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDEEL"
     gene            complement(196..339)
                     /locus_tag="VYS74_001766"
     CDS             complement(196..339)
                     /locus_tag="VYS74_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV3789764.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KNT"
     gene            573..2291
                     /locus_tag="VYS74_001767"
     CDS             573..2291
                     /locus_tag="VYS74_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV3789765.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            2402..2749
                     /locus_tag="VYS74_001768"
     CDS             2402..2749
                     /locus_tag="VYS74_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3789766.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(3027..3863)
                     /locus_tag="VYS74_001769"
     CDS             complement(3027..3863)
                     /locus_tag="VYS74_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3789767.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3876..4505)
                     /locus_tag="VYS74_001770"
     CDS             complement(3876..4505)
                     /locus_tag="VYS74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3789768.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(4515..5156)
                     /locus_tag="VYS74_001771"
     CDS             complement(4515..5156)
                     /locus_tag="VYS74_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3789769.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(5302..6531)
                     /locus_tag="VYS74_001772"
                     /pseudo
     CDS             complement(5302..6531)
                     /locus_tag="VYS74_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(6521..7687)
                     /locus_tag="VYS74_001773"
     CDS             complement(6521..7687)
                     /locus_tag="VYS74_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3789770.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7831..8844
                     /locus_tag="VYS74_001774"
     CDS             7831..8844
                     /locus_tag="VYS74_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV3789771.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEKGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         2808 a         1958 c         1569 g         2559 t
ORIGIN      
        1 tgggtcttgt tccttaaagc tcctcatctt tttttaaagt gaccttaatc tgctttaaag
       61 ctgcccatac tgaaggaaca gcacaatcaa aatgtgccgc aatttcccgt aaaaaagcat
      121 ctggatgagc ctctacaaag gctttcaatt cctctaaagg aatctttcgg tttttgacga
      181 ctcgcttttt cctctctatg tgttcttgtt cacgtctttc ttttcccacg tgaagagagt
      241 tctgacgcca acaccaaaaa acttggctgc ctcgacatgg ctgtgcccct ctttgatgta
      301 atctaatgct cgctgtttaa aatcagtact atatgccata gctttattat aatatgcttg
      361 tttaaaacta agtaactata tcaatgtttt gttatgagaa gacttacttt ttttactgaa
      421 attgaatttt tcctagcctc aacatcatat aaccccaaaa ttcaatctga cctggtttga
      481 agagattttc gaagcagata aggttttact aaaactaaaa aaaatgatat aattaaactg
      541 attaaatgtt ttaaaaataa aggagaattt gaatgtctta ccaagaaaat taccagaaat
      601 gggttgattt tgtggagctt cctgactacc ttcgtcaaga tttggaaaat atggacgaaa
      661 aaactaagga agatgccttc tatacaaatc ttgaatttgg tactgcaggt atgcgtggct
      721 tggttggtgc tggtacaaac cgtatcaaca tctacgttgt tcgccaagct actgaaggat
      781 tggctcgttt gattgagtca aaaggtggaa acgagaaaga acgcggtgta gcaattgcct
      841 acgatagccg tcacttctca cctgagtttg cctttgaatc tgcggcagtt cttgctaaac
      901 acggcatcaa atcttacgta tttgaaagcc ttcgtccaac tccagaacta tcatttgcag
      961 ttcgtcatct caactgtttc gcaggtatca tggtcacagc cagccacaac cctgctccat
     1021 ttaacggtta caaggtttac ggtgaagacg gtggacaaat gcctccacac gatgcggacg
     1081 ctttgactac ttatatccgt gcaatcgaaa acccatttgc agttgaagtt gctgatgtgg
     1141 aaactgaaaa agcttctggc ttgattgaag ttatcggcga agctgttgac atagaatacc
     1201 ttaaagaggt taaggacata aacatcaacc cagccttgat tgaagaattt ggtaaagaca
     1261 tgaagattgt ctacacacca cttcatggta ctggtgaaat gttggctcgt cgtgctcttg
     1321 cccaagcagg atttgactct gttcaagttg ttgaagcgca agcaactgct gacccagact
     1381 tttcaactgt aacatctcca aacccagaaa gccaagcagc ctttgccctt gctgaagaac
     1441 ttggtcgtca agttggtgca gatgttcttg tcgcaactga cccagacgct gaccgtgttg
     1501 gtgttgaagt tcttcaaaaa gatggtagct acctcaacct ttcaggtaac caaatcggtg
     1561 ctatcatggc taaatacatc ttggaagctc acaaaaacgc tggaactctt cctgaaaatg
     1621 ccgctctctg caaatctatc gtttcaactg acttggtaac gaagattgct gaaagctacg
     1681 gcgcaactat gttcaacgtc ttgacaggtt tcaaatttat cgctgaaaaa atccaagaat
     1741 tcgaagaaaa acacaaccac acttacatga tgggatttga agaaagcttc ggttacttga
     1801 ttaaaccatt tgtacgtgat aaagatgcca tccaagccgt tcttgtcgtt gctgaacttg
     1861 ctgcctacta ccgttctcgt ggtttgacac ttgctgacgg tatcgaagaa atctataaag
     1921 aatatggcta ctacgcagaa aaaacaatct ctgttactct ttcaggtgtc gatggtgctg
     1981 aacaaatcaa agcgattatg gctaaattcc gcaacaatgc tccaacagaa tggaacgcaa
     2041 cagctatcac tgtcgtagaa gacttcaagg cacaaactgc tactgttgct gacggtactg
     2101 ttacaaactt gacaactcct ccaagtgatg tgttgaaata cacacttgct gacggttcat
     2161 ggattgccgt tcgcccttca ggtacagaac caaaaatcaa gttctacatt gcagttgtag
     2221 gggaaaccaa cgaagaatca caagctaaga ttgctaacat cgaagcagaa atcaatgcat
     2281 ttgtaaaata aagacctata aaagatgaga ccaaccaacc tcatcttttt tcgtatcaaa
     2341 tctaagcaat tttagaaact ttatggcata ctagacttat caatgaaagc gaggtaatct
     2401 catggaacaa tttttggata acatcaaaga ccttgaagtc actacagttg tgcgtgcgca
     2461 agaagctctt gataaaaaag aaactgcaac cttctttatc ggtcgcaaaa cttgccctta
     2521 ctgccgtaaa tttgcagata cattgtcagg tgtcgtagct gaaaccaaag ctcacattta
     2581 cttcatcaat agtgaagaac caagccaact caatgatttg caagcattcc gctcacgcta
     2641 tggaatccca actgtaccag gttttgttca tattacagat ggacaaatca acgtccgttg
     2701 cgactcttca atgtcagcac aagaaatcaa agatttcgca ggattgtaaa aaatcagcta
     2761 acaagtctta aaatcacaaa aacgcataat gtaaggtgca aaaaccttga tattatgcgt
     2821 tttattgttc ctaaaacagt tacatcagca tttcattaca gaacatttta attttggaag
     2881 tcaaatcaat ttttaacaat gttttagaaa tcacagtggg ctattccata ttcaattatc
     2941 catattcaat cgctattatc gttttcctag attacaaaaa aactacgttt tgctaatcaa
     3001 aacgcagttt cagccactat tttatcttac tgtccttctt ttacttcttt ggttgctaca
     3061 tcttctccaa accatttttg gctgatttct tggaacttgc cgtccttgta aagactagaa
     3121 aaagcttcat ttatcttctt aaccaaggtt gtatcttcct tacgggctcc aaccgcaaaa
     3181 gcttctgttt ctagtccaac tgtaaagaca ttataatcgt ttaaaacacc ttctgcttct
     3241 aaataatagt ttgcatagac acggtcaatc aatagaccat caattcgatc gtttttcaaa
     3301 tcaatcaagg cttcattaaa ggtttggtat tgattcgctt ccttattagc gacaatattc
     3361 ttcaaaattt ctggatttgc ttcaaagtcc gcataaccag atgaaccagc ttgagctcct
     3421 aatgtctttc cagtcatatc ctttgcagtc gtgataccag atgatttctt cgtaaccaat
     3481 acctgctcat tcttcatata tgagttactg aaagccacct tttcacggcg ttcgtctgta
     3541 gcggaatagc cattccaaat cagatcaatc gttccttttg tcaattcagc ttctttcaaa
     3601 tcccaatcaa tcggttgcca atttaccgtg attccgtatt tttcaaaaac agctgtagct
     3661 aaatcaatat caaatcctgc ataagaacca tctttctgag caaatcccat tggaacaaaa
     3721 gtactatcaa atccaatagt aatagacttg ttagactggt actttgacca attatctcca
     3781 ctagtttcgc tagaattttt cccacaagct actaagaaca aagcagtcat cagactgact
     3841 aatacaagca tccatttttt catccatttt tcctcctatt taggttctac tttcaataat
     3901 acatctgcga tattttcagc aaactgcaaa tcatgggtaa ccacaatctg ggtcatccca
     3961 agttccctat tttgcaagat tagcttctcc acttccaaac gtaattctgg atccagggca
     4021 gaagttggtt catcgtagcc aatgatttct gggtcaatca tcatagcacg cgccaaagcc
     4081 acccgctgct tttgcccacc agatagtgag aaaggatagg cctctgcgtg tcctcctagt
     4141 cctaactgtt ccaagagttc actcgccttc ttctcagcct cttcctgctt cattcccatg
     4201 gtcttcacag gcgataaagt caaattttcc agaactgata gatgaggaaa tagttgaaaa
     4261 tcttggaaga caaatcccag taggttgcgc ttctgcaatt catccagctc taaaggttgt
     4321 ccattataaa agatttgccc tgaatcaatg gtttcaagac ctgcaagcat acgtaaaaga
     4381 gttgtcttac ctccaccaga aggtccaacg atagccagga tttgcttttc aggaatactt
     4441 agactgaaat cagacaggat ttgtttgtct ccaaagactt tattgatatt tcgtaattct
     4501 aacatagctg ccccctatct ataataactg tactttttct caactttttt ggaaataatt
     4561 gtcacaatcc caattaaaat caagtaaatg gctcctgcca agaacatagg aaccagacta
     4621 gcatcgcggt tagcagctgt tcgactagcc aagataaggt ctgaaattcc gagagcatag
     4681 accaaagaag tatccttgac caaactcata acttcattaa agacactagg aagaacgatc
     4741 ttggtcactt ggggcaagat aatatagcgc actctgtcaa aagggctaaa cttcaagacc
     4801 ttggcagcct catactgtcc tcttggaata gtgtcaattc ccccacggaa aatttctgca
     4861 aagtaagctg catagttgag aacaaaggca ataatagctg caggaaggcg gtctaaacga
     4921 atcccaatac ttgggagcac ataatagata aaaatcagtt gcaagagtaa gggggttcct
     4981 cgcataaccc agatgtaaat gttaatcaga taatggaggg gcttccaatg gacttgcaag
     5041 gcaaaggcaa tcaaaacgcc caagggaatc gaaaatagca agaccagtgc aaagacctgt
     5101 acagtcgtgc tcgcaccatt cagtaaactc ggtaatatct caaataaata agacatactg
     5161 cacctcctaa aagtaattgt tcctattata gcatgaataa acaagattac caagtatttt
     5221 tgtaaaaaga ttctaccata tacaaataaa aaccgtagct atcaaaagag ataaactaca
     5281 gttctatatg tttaaacatt cttacaatct ctcaaacagt tcttgcattt gcacatcatc
     5341 tggaaccagt tttaagtaag tgtgagcatg gacttttgct tcttcaaaat gtcccaattc
     5401 acgcaagaga tagatatagt gttccagaaa ttctggattg tccttcaaat ctcctgtcaa
     5461 ctcttgataa tgctcataag cagtatccaa atcgtccatt tcttgataag aacgagcaat
     5521 catccacttg gtcaaaagat tttctggctc ctcactctgc aagtctagaa tatcctcata
     5581 acgctcctgc tccagataaa tagtggctaa acgaagcaag atttcttctg tatcctcagc
     5641 gtcttctttt gcagtaagga gataattttc tgcaccacta gcatcatgca attcataaga
     5701 aaattgtgaa gcagctagca agaggcgagt ttcaaaggga tttttctcta atccttgctt
     5761 agcgatacgc agggcttctt gaacttgatg ttccttatgt aaagcctgac tgtaccctta
     5821 ctcatagcct tcaaagtcag gagaaatggt atcaagctgc ttaaagtaga gggtggcttt
     5881 ttgatattct tcttgatcaa aataaagact ggccaactca aaagctgtta agtcatcgta
     5941 ttctaactcc agggcttttt ctaaaaactc agtagccgtt tcaaatttcc ctaactgagc
     6001 ataggcaaag ccaattcgtt gataggtgga aatgcccgtt tgctcataaa tcgagcgatt
     6061 atctaactgg gcataggctt gaatagccgc ttggtaattt tccaactcac tatccaactc
     6121 tgccaaaccc aatatcaaga gagaatcctc tgagtaggtc aaggcctcca ataatttctc
     6181 acgtgccaca tctgtcaaac cttccagctg gtaaaggtct gccttcaaaa ccaaagacga
     6241 gacataccag tcactgtcag cttggatttc ctcaagatag gtaaaggctt cttctatttg
     6301 accatcctcg ctagcaattg cagctagatt aagatgaacc tctggaaaat cctctacaat
     6361 tttcaggtaa atttccttgg cctgaggata gaaaccaatc ccttcaagat aagttgccaa
     6421 ctcatacaga agattacttg aatcattttc taaagctttg gcgaaataat gctcagcctt
     6481 agttaaatct tgctcctcca aagcctgtaa catctgttga ctattgttca ctcttgactt
     6541 cctcaccctc taattcatat agaccactga ctaccttata ccattcgaaa atggctgaaa
     6601 tgacaacctt agcagaggca taaaccggaa taccaagtaa aactccccag ataccaaaca
     6661 tagatcctga agttaacaaa acaaagagaa cattaatagg gtggatgttt aattgacttc
     6721 ccaaaatcaa tggagagaca aaacggcctt caatagtttg ttctacgata aagacaatca
     6781 ctactttcaa aagcatgact ggaccagcaa tcaaacccaa tactagagca ggaagcatgg
     6841 ctagaaagct accaagataa gggaccagat ttaaaatacc agcagtaacc cccagcgtaa
     6901 ccgcatagcg tagaccaata atcttgaaga agatgataaa cattactgct acaataatag
     6961 ccactgtcac ttgccctcga acatagttgg acaactgttg attcacatct gatagaactt
     7021 gtccaacagg ttccttcaat tttcttggaa tgaattgggt caaatagtta cgcaagcctt
     7081 tcccatcacg caagagataa aagagcatga aaggaacgat aatcaaggca acaatcactt
     7141 gagaagctcc gctaataaag gcactcaccc agttgactgc ctgagatgaa accttacttg
     7201 ccaaaactgt agcctggcta gaaaaattgg tcaaaacttg ctctaattga ggtctgaaat
     7261 catctggcag gtgctgggct accaatccat taacaatcct atctatatct tctaagtaaa
     7321 caggaacgtt tcttgcaaag gtcaaaacct gacgttgcag atttggaatg gcgactgcca
     7381 agccccaaat gataaagaga gcgatgataa caaagacaat agtgatagct ataacacgat
     7441 taaccttatg cttctccatc caatcaacaa taggattcaa caaataatat aacaaaccag
     7501 acaaaatgac tggcaacatc acaactgcta aaaagtctaa aacaggtgaa aatagaaaac
     7561 taatcttact taaaataaaa agattcagtc ccaataataa ggttactaaa aataccgtaa
     7621 ttgccttgtt atctaaaaac cacttgaaaa accaagatag gctaaaatgt ttctcttttt
     7681 gctccataaa tacttccttt ctattgttct ctcattatac catttttgaa acaaaataac
     7741 tcttttttaa agtgcttaca tctaattgga tatctttaat ttacttgttt ttgtgatata
     7801 atagtcttat ctttacacag aggtatagta atgaaagaaa ctgtttattt tggaacttat
     7861 acacgtcgta cttctcaagg gatttacaag gcagactttg atacagaaac tggtcagctt
     7921 tcaaatctag aactttttgc agctgagcca agtccaacct accttgcctt tgaccagcac
     7981 caacatttat acactgttgg tagccaagac gataaggggg gaattgcagc ctatcaaact
     8041 gacgggactg tgttaaatca tgttgttgaa aaaggagctc cccactgtta tgttgctgtc
     8101 gatgaaaagc gtgatttggt ttacgcagcc aactatcaca agggacaagt ccttgtttat
     8161 aaacgccagg aagatggtag tcttctactt agtgatatgg atcaacacag tggccaaggt
     8221 ccacatgaaa atcaagcttc cccccatgtt cactatacag atttaacacc tgaccactat
     8281 ctagtgacct gcgacttggg aactgaccaa gtcatcacct atgacctcga tcaagaagga
     8341 aaattatcta agctctatac ctatcacagc aagccaggag caggctcacg ccatatcatt
     8401 ttccataacc actataaaat cgcttatctc atttgtgaac tcaatagtac tatcgaagtt
     8461 ttaatctacg atggcgttgg cgaatttgaa cgtatgcagg tcatttcaac tttaccggaa
     8521 gcttacgaag gctttaatgg aaccgctgct attcatctct ctaaagacgg taaatacctc
     8581 tacgcttcta accgtggcca tgattctatc gcagtatata ccatccttgc ggacggtagc
     8641 ttagagttat tagaaatcgt tccaacgcat ggtcagactc cacgtgattt tgatttgaca
     8701 cccgaccaaa aatttctcat tgttgtccat caagactctg acaatgcaac tgtctttaaa
     8761 cgtaattgtg acaatggtcg tctagcagaa ctctccaacg acttccatgt tcccgaagca
     8821 gtctgcatcc gttttgctcc ttaaaacacc ataaaaaatg gttctataat atttgtagtg
     8881 ggtaaatccc ctat
//
LOCUS       DAVMMR010000069         8686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMMR010000069 DAVMMR010000000
VERSION     DAVMMR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.069"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            436..2265
                     /locus_tag="VYS74_001775"
     CDS             436..2265
                     /locus_tag="VYS74_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV3789772.1"
                     /translation="MRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYI
                     LSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPD
                     FLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFD
                     GTYSSEKLASECKALKILNEWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFP
                     LYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWE
                     QAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVS
                     QTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESE
                     QDIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLA
                     KKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELF
                     TTHFIEIFENSQNKNLYSGQAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIEN
                     PETFCKVGASDVQSNDPYENFGGLLYGHAGVAWLFGEAYKLTG"
     gene            2290..2754
                     /locus_tag="VYS74_001776"
     CDS             2290..2754
                     /locus_tag="VYS74_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic synthetase"
                     /protein_id="HEV3789773.1"
                     /translation="MAVDKELVAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRN
                     KEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVANNIYSDIDDNFSGQKKLIE
                     QLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLVSIMDNKLTILPQI"
     gene            2787..4727
                     /locus_tag="VYS74_001777"
     CDS             2787..4727
                     /locus_tag="VYS74_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV3789774.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4724..6331
                     /locus_tag="VYS74_001778"
     CDS             4724..6331
                     /locus_tag="VYS74_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789775.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLLVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6346..7086
                     /locus_tag="VYS74_001779"
     CDS             6346..7086
                     /locus_tag="VYS74_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789776.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7089..8408
                     /locus_tag="VYS74_001780"
     CDS             7089..8408
                     /locus_tag="VYS74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789777.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            8542..8679
                     /locus_tag="VYS74_001781"
     CDS             8542..8679
                     /locus_tag="VYS74_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV3789778.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         2998 a         1077 c         1628 g         2983 t
ORIGIN      
        1 agttatggat ctcgttccaa tatgtgcacg ttggaatgtc agtgcttata tggggggaat
       61 ccaaaggatt ctcccttttt attttaataa gtctgtctag ctatagatat attaagtttg
      121 atagaatttc ttggagattt tgttatggat tataatttca atttggagca tcctttcttt
      181 ttcactaata atgattatag tacagatact agcataaagt actaagtaag tctgcctttt
      241 aattggcacg aagtaatgaa taatgatgag tgggtttatc aataccctat tggaaaattt
      301 gttgaaagac aagggtggaa aatacacatt agttcagagt ataatagttc acacgaattg
      361 ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc catttaaaca tcttagtaca
      421 gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc gaggattttc tggtaaattt
      481 attacatgtt atccaaatca aaatgaatta gagtcggttt tgcaaaggtt agaaagtgcc
      541 ttaaagcaat ataatgggcc atatatttta agcgataaac gttgggatga agcaccaata
      601 tatctgaggt atggtgtgtt taggccgtca agggatgatg aaaagaaggt tgctattgat
      661 gaattaattg ttggcgatga agttgttaaa gatgaacgtt taccagtatt taaaattcca
      721 aaaggtatag tgccccctga ctttttgaat aaatggcttg acaagaaaga taaaaaacag
      781 ggagactttc cctttattat agataatgca attagatttt caaacagtgg tgggatatat
      841 aatgctaggc taaaagaaga tgggaaaaaa ataattttaa aagaggctag accgtataca
      901 ggtctaggat ttgatggtac ttattcttca gagaaattag cttcggagtg caaagcacta
      961 aaaattctaa atgaatggag tgagacgcct aaaatatact ggcacggtaa gatctgggag
     1021 cacaccttct taggaattga acatatgaag ggcgttccac taaatagatg ggttaccaat
     1081 aattttcctt tatatgaagt tgttgataag accaaagatt atctattgag agtttcaaag
     1141 atagtagaaa aactcatcga tttaacgaat aagtttcatt ctgaaaatgt atatcatcaa
     1201 gatttacatt taggaaatat cttggttaag gatgaagatg agattagtat aatagactgg
     1261 gaacaggctg tcttttcaaa tgatgaaaaa gttgtacata aagttgcagc ccctggtttt
     1321 agagcatggc gagaaacact tcccagtgag atagattggt atggtattag acaaatagct
     1381 cattatttat acatgccact agtaactaca tcggatttga catacaacta tgtaagtcaa
     1441 accaggatag aaggaaaaaa actatttgag tctttgggtt atacaagaga acatatagat
     1501 tatgtagaaa gtttattgtc atatctagat tctaaatgtc cacagattga gaatatttcc
     1561 aggaaaaaag ttttaaaacc catgcatgag attagaacta tcgagagtga gcaagatata
     1621 caagatttta ttataaaatt attgagaggg tttacgctaa cttatggaca gtggcgtaaa
     1681 gaatttcaat caagattctt cccagttcat tattatggtc tgaattttaa tcaaggaatt
     1741 gcgttttcag atttagctat cctttggagt tatcaacaac tagctaagaa agtaaaaaat
     1801 ttcaagtttg atgactatta tgagattcga acacaggtta ttaatgaggc agtaaataat
     1861 tttaagaaat cgtccctttc tggtcttttt gatggtaaaa ttggtacaat ctggctcatt
     1921 tatgaatttg gggagataga tcgggcagtt gaacttttta caactcattt tatagagata
     1981 tttgagaatt cacaaaataa aaatttatat agtgggcagg ctggaatttt actggttgga
     2041 ctgtattttc ttagtaaagg ggaaattgat aataaactag gagaagaaat actaattagg
     2101 cttagagaat atactttaaa ttatatagaa aatccagaaa ctttttgtaa agttggcgct
     2161 agtgatgttc agagtaatga tccttatgag aattttggtg gattactata tggtcacgca
     2221 ggggtagctt ggctcttcgg tgaagcttat aaattaactg gttaaagtat ctataaaaat
     2281 ggtctagaat tggctgtaga taaagaatta gttgcttata aagtagatag taataattca
     2341 ttgcagtaca gccaaggtca tagattacta ccatatctag caactggaag tgctggcctg
     2401 cttttactta ttaatagaaa taaagaaatc ttgtcaagca aatacttaaa atatttaact
     2461 tctttagaga gggcaactga tgtagttttt tgtgtattac caggtttatt taacggattt
     2521 tgtggtcttg aagttgctaa taatatatat agtgatatcg atgataactt ttcaggacaa
     2581 aagaaattaa ttgaacaact ttatagatat ttgtgtgtaa ttgaagaagg atttgttatt
     2641 gcgggagata atggtttaaa gataacaaca gatattgctt cgggatttgc gggtgttgca
     2701 attggattgg tatcaataat ggataataaa ttgacgattc ttcctcaaat ataatattta
     2761 gataccatta ttaaggggtc atacttgtga atcagagtaa tgaaatattt ttaaatacaa
     2821 tacaggaaaa aacacactat aagaggtcta tctcttcatc atttcaggaa ttaaaaaaat
     2881 ggaattcttt gatatttagg agagggcgat attggtacca atttgataat tggagtatac
     2941 ccaatactgg tgtaataaag gaatggtatg tcgaagataa tgactggatt cctaaggtta
     3001 gttcttccag tttagacaat tcaaattcac ttaaaattgt ctttgatgtg gaaaaatata
     3061 ctttgccaga tgatagtatc ttacaggatg ttaggatttc atctgatgag agttatatag
     3121 ttttggttgt atcctcactg cgtagcacta atttaattgg aataaaaaaa tatactatgg
     3181 aagagttatt tatcattgaa gatatttcag tagaaagttc gttctatttg ggtaaattcg
     3241 gtgttatgta tactcgctcc aaagagtacg ggaggccaag taagttattc tataaatctt
     3301 ttgatagctt tacagaagag gaactgtttg aagaaaacga atgctctttt cgattaaaaa
     3361 tagtccacat tgatagcaat aattgttttg taaagtcagt agactttcag aaaggcagga
     3421 tttttctata cagtttcgac cggacgggtt ttgttagaca ctcttacaca gaaacggtag
     3481 ccccaacacc tagagatatc gctctattta gtacagaaaa agcacagtac tttcttgggc
     3541 tctcttcaac tgaagaaaag aaagatcaac taattctaag ggaaacttct tcgggagaca
     3601 gagtttctat tactatacca tatcaagata gggctagaag agttcactgt ataggacgtt
     3661 atatcctttt agactgttca aacgcagcga attccgtctt ttatcttgct acatttaaaa
     3721 ataatagttt gagggagttg agtgttaata agataacatt tgatgaaaag acaacacttt
     3781 atgaaaattc attttctgat cgtgttcttc tgtttttaga gaggagaact ttttacgaaa
     3841 aaatacttag ttttgattta atagaaaata cattaaaaac tgaatttgaa agacccatca
     3901 taaaatcgaa taatactaaa tatttttcta aggtgatttg gactaaaaaa gacagttatg
     3961 atgtaagcat accgatttct ttattttgga aaagtgaaga tacggatgag ctcccaagaa
     4021 gaaaaaaatg tattctaagt gtctatgggg cttatggtaa gaatgataat tctgatttag
     4081 atgaaattat gttatctata atagatgcag gttttattta tgctatagtt catgtaagag
     4141 gtggaggcta tttgggtggt gaatggtatc gctcaggaaa ggcgttaaac aaatggaatt
     4201 ctatcagaga ttttattgag ggagtaaatt atttaaggga aaatgatgta attgacagta
     4261 agcgattagg tttaataact tctagtgcag gtggaataat tgctggtgcg gtgttgaacg
     4321 aggaaaaaaa tttattgcaa agcattctct tattttcgcc atttataaat ccttatgata
     4381 cacttcagaa tccaaatgat cctctttcta agaccgaaat agcagagtgg ggagatatta
     4441 gagaccctga agtaaaggct tatataaagt catattcgcc tatgcaaaat attgaaaagg
     4501 cacgagactc aaatactgtt atagttaata ttttgggtga gaaagaccca tatattaata
     4561 ataacgaagt gatagagtgg tcaaagaaat taaattctat tggagttaaa agtttgttgt
     4621 atttaaacaa agcagctggt catggaggtt ttactccatc agatgttctc ttaatgattg
     4681 atactttaaa ttatttcttt gaagaagtgg gaaggaataa cttatgaatt atttaaaatt
     4741 tataaaaaag actaaactta tattaatggg gattttaata tttctatctt cgtttaatgg
     4801 tgtcttgctc tcaggaatta ttgtatatgc aggtagttta aatcagactt cctcattttc
     4861 tgacgttcta agatttggtg ctataagtat tctgggttgg tcagcaatct atatatcaaa
     4921 ttactattta gaagtaacgg aagcatcaat aacgaaagat ataaatgtaa aaattaaaca
     4981 aggatatttt agagaacagt atctttcttc tgaaatggtt aaagattact catctattat
     5041 ttcagttttg tcaaatgatt tgagattaat cgaagaaaac tattttagac aaatttttga
     5101 aataatttct tcaatattgt tgttcatcgt ctctctaagt tttatgcttt atttaaactt
     5161 cctagtttca ataatattta ttgtattatc agcattgccg attatagtac ctgtctttat
     5221 gaagaagatg ttgtctaatt cagctaatga gtactctaac agcaatgcag agtatactca
     5281 cataataaaa gaaattttta atggttttaa gacattaaaa tcttattctg ttactaaaga
     5341 aataattagt ttgtcggata aaaagttgga taaactagaa gattctactt ttaatttgaa
     5401 acgatcagag gttctttcaa aattggttgc agtactaatt tcaggttttt gctttctagt
     5461 tcccttgctt gtaggatgtt attttgtaat ttatcataaa agcctttctt ttagtgaact
     5521 gataggtatt ttcttagcaa acgataaagt tttagggccg atacaatcaa ttgcctattc
     5581 attaaataag ataaatacaa ccaaagattt aaggaaaccg tttttaaaat acttaagtgg
     5641 agagaagaat tttatagacg ctgaacatga taataacgga ctgtatactt catcaataga
     5701 tgagatacac atgaaagatg ttgtatattc tattacacca gaaaataaat taagtattga
     5761 cttctcattt aagtcaccat ttagggtatt attaacagga acttctggta gtgggaaaac
     5821 aacgatttta aatttaatta atggttcttt aaagccacaa aaaggttatg taaatttgtt
     5881 atcacatggg aaaaagagtt cagattcaat accaacagtt gatcagacac catatatttt
     5941 tgacactact attcgtgaga acgtaacttt atttcaaaat gaatattttt cagatgatca
     6001 gataattgag gtgttaaaaa aggtaaatct atatgaagaa ttagaaaaga tagatatact
     6061 aaattatcaa tgtggtgaaa atggtagtaa tttgtctgga ggtcaaaaac aaaaaatagc
     6121 tttagctaga gctctgatta gaaataataa agtgtactta tttgacgaaa tatcagctaa
     6181 tttagataat gataattcaa attccataca tgatattctg ttcaatttag gtatttcatt
     6241 tattgaagtt tcacatcatt atgacttaaa tgacaagaga tacactgata tatataaatt
     6301 ggaaaatgga acacttttca aaattaagtg attggaggca agaatatgga taaagattat
     6361 atattaaaag tgaaagggct gtatcatcaa tttttactag gaaataataa aacgttgcaa
     6421 gtgctgaaaa atgtttctct ttctgcttcg agaggagaat ttataagtat tctaggaatt
     6481 agtggttctg gaaagtcaac tttattaaaa tgtatttcta gtttgcttga accaacaagt
     6541 ggggaagtaa ttttaaatgg aatcaacccc tataaaatca gaaatgcaaa attgtcaagt
     6601 ataagacgta acgaagtatc ttttatattt caagcataca atttaatacc ttccctgccg
     6661 gtaatagaaa atatagcact tcctttgcga ttatcacaaa aaaaattaac tattaaaaat
     6721 gtagaaaact tactcaaaag aatgaagttt aatgctggct taaacgattt tgttggaact
     6781 ctgtctggtg gagaacaaca gaaggttgct atagctagag cggttattgc tgatagtgat
     6841 ataatatttg ctgatgagcc aactggggct ttagacagcg tttctcgtga agtaattttt
     6901 gaattattga gagagttagt aggggcgggt aagtgtgtaa ttatggtaac gcacgatata
     6961 gaattggcct cgaaaactga tcgtgcatta atattgaaag atggaaaaat tttcaaagaa
     7021 cttcatagac ctagcgggga agagttgtat aaaatcttag aggtacaatc aactacggag
     7081 gaatagagat gctcagatta gtttactatc aatttttaca taataagaaa caatggttag
     7141 gcgtttcacc agtaattttt gtttcgagtt tagttatggg attggctgtt aatggggtaa
     7201 ttaatgttga gaataattcg caagtatttg tcggacttcc agatcctaaa ccaattttta
     7261 tgtttccgat agtattcgga ggagtaacat tattctttgt gctctctaac ataataaata
     7321 tgttggttga aatttttaga gatgattacg aacttctgga ggtgttggga gcaagtctcc
     7381 ttcaactatc atttcttgtg gggggacaga tttttatcat ttcctcgatt attagtttta
     7441 ttgcatatct atgctctata tttgttacgt caaattatta ttatttttta caatactttt
     7501 tcggggaaaa tatattacct gatatccaat ttcaaacgag tgctgttgga tgtatcatta
     7561 cagtagtgtt gatttcattt ttagcctttc taagtggttg cttttatacg ttcaagaaga
     7621 ttagaaatag gaagagttct aaaataaggc atgttctttc aatagtaaaa agaatactgc
     7681 tattggctgg ttttagtgtt atctggttgc tttctttgca acaaattttc caagatagca
     7741 caattttagc gaaggcccag ataatattca atattgtaat cttagatatt gtgattattt
     7801 atcaattatc tccttttata cagtcatgtt ttatcaaact actttctata attatattta
     7861 gaaataattt tatgttcatt gtttcgaagt ggaatttact atatcgtaag ccttatataa
     7921 aatctatcag tgctgcaatt acaggagcta tattactaat ttctagtttt caaatgattt
     7981 ctcagaatat actatctcaa tttcaagatg acagtgattt agagctaaaa gtggcattta
     8041 ttgtctatgt aggagcacca attttgatag tacttgcaaa tattatttca attgcatttt
     8101 tgtcatcgca tcaagaaagg attgagattc agcaatttga aattttagga acttctaatt
     8161 accagatggt taagattaaa gttggtgagg ctatattttt gacatttgtt acttcattaa
     8221 ttgcattttt acttaacatt aaaattatgg ctttaatata ctactcttta gaggatattt
     8281 taatagatga tatgaatctt ttggggttaa tacttccaaa ttttatagtg tctattattt
     8341 tatttatttt aatatttatt actaaaagtt cctattttat ctttaagaat gcaaaaatta
     8401 tttcttaaaa atttgtaagt tttcgctata taaaatagtt aaatctatct tgtatcattg
     8461 cttgcatttt actggtggag agaatataat gagggtggat aaagtactta atcccaaaaa
     8521 tttaaatagg aggagctagc tatggcagaa gaagtattaa atcttcaatt agtatctgtt
     8581 caagttgacg aaacagatga agttgatgga atgcgattta gtacattcag tacaaaccgt
     8641 tgcggtaact ggagtgcttt tagttgggaa aattgttaat tcattt
//
LOCUS       DAVMMR010000070         8619 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMMR010000070 DAVMMR010000000
VERSION     DAVMMR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.070"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(161..643)
                     /gene="greA"
                     /locus_tag="VYS74_001782"
     CDS             complement(161..643)
                     /gene="greA"
                     /locus_tag="VYS74_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV3789779.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(714..2369)
                     /gene="mltG"
                     /locus_tag="VYS74_001783"
     CDS             complement(714..2369)
                     /gene="mltG"
                     /locus_tag="VYS74_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV3789780.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2450..2944)
                     /locus_tag="VYS74_001784"
     CDS             complement(2450..2944)
                     /locus_tag="VYS74_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789781.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2941..3405)
                     /locus_tag="VYS74_001785"
     CDS             complement(2941..3405)
                     /locus_tag="VYS74_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3789782.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3415..4749)
                     /locus_tag="VYS74_001786"
     CDS             complement(3415..4749)
                     /locus_tag="VYS74_001786"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV3789783.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4760..5377)
                     /locus_tag="VYS74_001787"
     CDS             complement(4760..5377)
                     /locus_tag="VYS74_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3789784.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5425..8523)
                     /locus_tag="VYS74_001788"
     CDS             complement(5425..8523)
                     /locus_tag="VYS74_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3789785.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
BASE COUNT         2351 a         1953 c         1653 g         2662 t
ORIGIN      
        1 gctcaaaaca tggttttgag gttgtagatg aaactgacga agtcagtaac catacatacg
       61 gcaaggcgac gctgacgtgg tttgaagaga ttttcgaaga atagcaaaat ggaaaaagga
      121 gtgagtgaag cacatcgcct ccccactcct ttttctgttt ttaggctgtt ttttcaacct
      181 tcaagatttt tacatcatag ctaccaacag gcgtttcaat ggttgctgta tcacctgttt
      241 tcttgccaat caaggcctgc ccaattgggc tttcatttga aaccttacct gcaaaggcat
      301 ccgcaccagc tgaacctacg ataatataaa cttcttcttc gtcctcacca atttcttgga
      361 tggtgactgt tttaccaatc gctacttcgt cctgggcaac tgcgtcgcta ttgacgattt
      421 cagcatagcg gatttttgtt tctaagctag agatttgtcc ttcgacaaag gcttgttcat
      481 ccttagctgc ttcgtactca ctgttttctg aaaggtcacc gtatgaacgg gcaatcttaa
      541 tgcgttctac cacttctggt cgacgaacca atttcaattc ttctaattct ttttcaagtt
      601 tttccttttc ctcaagggtc ataggatatg ttttttctgc catttttctc aactttcttc
      661 tgataatatt ttctaaagaa aattatgtga agtataacat aattttagtt tgtttagttt
      721 aatttgctgt tgacatgttc agcgacattg cggtcgtggt cttcttgatt gttagcatag
      781 tagaccttgc cttctgtgac atctgctaca aagtagaggt tatcgctctt agtttgattg
      841 atgcttgact caatcgcatc cagacttgga ctatcgactg gaccaggcat gagacctaca
      901 tttttataaa cattataagg tgaatcaatg ttggtatcaa tcgcaacatc ctcagctaga
      961 ctgatatttt gccccagttt tccttgggca tacaagattg caatattact ttgaagtggc
     1021 atatcacgat tcaaacgatt gtagaataca cctgcaatga gcttacgatc ttctgtcttg
     1081 gcaccttctt tttcgaccaa ggaagcaatg gtcaacaact cattgacagt caagttttta
     1141 gatttgatag tactatagta aggagatagg ttcttatcca tagcagctaa catctcatca
     1201 atcaagctct caatagttgt gctttccttg atagagtatg tagctgggaa aaggtatcct
     1261 tccaaacgat aacgcgcacc gctgtctttt acaggcaaac tttccagtaa agtaggatat
     1321 ttcgctactg cttgactgat aaacgtctca tcttgaactt tagccaagaa agtctccgct
     1381 gtcaaagact ctttgaagtc accttgcaat tgacccacag cttgagcaat ctgatccaag
     1441 gtataacctt ctggaattgt caaagtcgca agtacaggtt cttgcggttc atctgttcca
     1501 cctttttgca actctttgag taagtcttct gtactcatac tcttttgcaa attgtagtaa
     1561 cctgctttca agtcggtata atttttatac ttggcataaa aactaaaaat cagaccatgc
     1621 tttaccaaac cagctttttc aagcgtcgta ccgatttctt gaacgtttga accttctgga
     1681 attccaaccg tcacatattt cttagaatta gcatcgatag gtaataagga atctagcacg
     1741 tactggtaac caaagtaacc acctgctgag agcaaggcaa ggaatacaag gaatgaaatc
     1801 acaaaagctt tcacatgtga cttcttctct ttcttaggct taacgggttt tgctccttct
     1861 ctacggctac gacgtgatgt atctctgatg atatccactg tttctgtagc aggcgtaggt
     1921 gtttctggac ctgcttgttc tgcttttttc tctgccgtcg tataggaaac agctaccctt
     1981 gttggggttt cattgtattc tctttcaagt ttcttaggtc taacaggacc tggacctggt
     2041 cttgatccac tttcttccgc tggagaagaa ggtacatctt gacttggatg acttggaaca
     2101 ccaggagttt ctctttgaat ctcatctgct ggagaagctg gtacaccttg acttgggtga
     2161 gtaggcacgg taggagcttt tctcataatc tcctctaccg ttgacaagga atcagccatg
     2221 agttcttcag ttgaaggttc atttgcagga gtgcgaacta ctgcctcatc ttctttcaga
     2281 acttcatcat agccttttac tttttctaaa tctctcagaa tctgctcttt aaagcttaat
     2341 ttctcttctt ctcttgactt ttcactcaaa agtttttcct ccttgttgat aatccataat
     2401 attatatctt aaaagtccaa aaaaagcaat ctatgatact tttcctaact tattttttca
     2461 tttcccagac catatcatac catgtttccc ctgcaaaggt tgactgggac tggccttcgt
     2521 tgacaaatcc atgtttttca tagtaggcga tgagatagtc atgacaggtt agattaatgc
     2581 catctctctc atcttcaaga gcaacttctt tcaaggctgt cagcaatttc tgacccagtc
     2641 cgagtccctg tgcctccttg gcaatagaca gacaggtcac agagatataa ccaccaggct
     2701 catgactata gtcttttatt tcttctgtaa aagactggtc ttgcagatgg cggtgcaggc
     2761 caactggtcc ttcgatataa cccatgattc ttccttcttt ttcagcaacc agaaaagagg
     2821 tctgaatttc tctcaaatgt gcttcaaaga cagaaggagg aatggcttct tcgaccgaaa
     2881 aattttcaaa ttcaagttca acaatccgat ccaaatcttc taatcttgct tgtctgattt
     2941 tcattgttcc tccagataaa agggattaaa ccaaatcata ctatagccct ggctagttgc
     3001 atagagcaaa gttgcttctt catcaacaaa accgttcatt tcaaaatagg aaagcagctc
     3061 atcaggactc tccaaacgaa tccctttgta atccagctca actgccacct ctttcaaggc
     3121 tgcaagaaga agtgttccca agccctgtct ctgatggtca gactcgatga ctaaagaatg
     3181 tacttttaga tattgcggat tgtctgactg gggacttgat aaaatatagc ctaaaagttg
     3241 attttcatcc ctagctagaa gaaaggtatc cgcacactta cggatacttt cttctaaaat
     3301 atgggaaagt tgctgctttt cagctggaaa agacgaggtc tgaagtgccc ctatctcagg
     3361 caaatcagac ttgcttgcct gaatgatctt aattggaatt tccatgggaa catcctattg
     3421 aacattgctt gtcaagttag acaagagacg ctcaaatgag tattcatagg tttggatgtc
     3481 tcctgctccc ataaagacgt aaacagcatt gtcatggtct aggagtggag aaacattttc
     3541 aacagtaatc acttggtgtt ttttgttgat tttgttggct aggtcttcta ccttaacgtc
     3601 accatgatct acttcacgag ccgagccata aatttgcgct agataaacag catctgcttg
     3661 gtttaaagca tgggcaaagt cgtccaacaa ggcaatggtt cttgtaaagg tatgcggttg
     3721 aaagactgct acaatttcct tgcttgggta tttctgacga gccgcatcca aggtcgcaat
     3781 aatttctgtt ggatgatggg caaagtcatc gataatcact gtatcattga caattttctc
     3841 agtgaaacga cgtttaacac cggaaaatgt tttcaagtgc tcacgcacca agttcaaatc
     3901 aaatcctgct gtgtaaagaa gaccaataac ggctgtcgca ttcatgatat tgtgacgacc
     3961 aaaggttgga atgtggaatt gccccaagtt ttgtccacgg aaatgaacgg tgaaggttga
     4021 accagttgtt gaacgaagaa gatcactagc tacaaagtca ttgccttcag cttcaaaacc
     4081 ataataataa attggtgcat cagacgtaat cttacgcaat tcagcatctt caccatagac
     4141 aaaaagaccc ttggtgattt gtttggcata gtcgttaaag gcattgaaaa catcctcgag
     4201 acttgtgaaa taatctggat ggtcaaagtc aatgttggtg ataatagagt attctgggtg
     4261 gtaaggcatg aagtgacgct catattcgtc agattcaaag acaaaatatt tggcattggc
     4321 cgaaccacga cctgtcccat ctccaatcaa gaagctggta tctgtaatgt gagacaagac
     4381 atgagacaac atacctgtcg ttgaagtttt tccatgtgct cctgctactc ccatgctaac
     4441 aaagtcacgc ataaagctac ctagaaactc atggtaacgt ttgtagctga taccattttg
     4501 gtccgcatag gcaatttcga cgttgttatc tggacgaaag gcatttccag cgataatttc
     4561 catatcaccg tctagattct tttcatcaaa aggaagaatg gtaattcctg cctgctcaag
     4621 accgcgttgg gtaaagtagt acttttcaac atctgatccc tgaaccttgt gccccatctg
     4681 gtacaacatc aaggccaagg cactcatccc tgatccctta attccgataa aatgatatgt
     4741 ctttgacatg ttttctcccc tattctgtca ttctggtcag attcaactct tgggcaaccc
     4801 gacgttcttg ttctgtttgt ttactttttt tattgtagat ttggctcttc tttagaaaat
     4861 cataattgtt tttctttgga gcaggtgctg acacttcttc attcttggta gggatagaat
     4921 gaacttcttc cgccaagata taatgagact gggtcaattt ttggctatat ttgacaaatt
     4981 caccaggatt ttccttttgg aaaggagttg tcggttgatt gccctgtcta actagactgg
     5041 gctgagaatg acgtctcgca aggctgaaat cctgagttag atagttagca gagcgtttct
     5101 ttttcaagtc tgcacgcgct tcttcacgcg ccacctccgc atagctcttt ccttcttttt
     5161 taacccctaa aggagctttt ttaggtttcc ctacttgctt ttcaatcggt tttactggtt
     5221 tttcttcagc aataggagcc cattctaaat aatttttatc tcgatactca cccttgatat
     5281 tactgatcag atcagactca tcatagagat tcatgactgg catttcagtc aacatgacct
     5341 cgtcatctga caccaatgga aatcgttctt gtttcatttt ctatttcctt tcaacacttc
     5401 attatagcgt attgtcttga tttttcaagt gctggcttca gaaattccca aaatttctct
     5461 aatttctgct agggtcagac taccacgtga ctctgtgccg tccaatactt gtgacaccag
     5521 atgtttcttt tgttcttgga gttcctgaat tttttcttca atggttccct tggtcaccaa
     5581 gcgatagacc tcaaccgttt cttcctgacc catccgatgg gcacggccaa tggcttgcgc
     5641 ttccaccgca ggattccacc aaaggtcaac caagatcact gtatctgcac ctgtcaggtt
     5701 cagaccgacc ccaccagcct tgagggaaat cagaaaggca tctctttctc cttggttaaa
     5761 ggccttggtc atgtcttgtc tttccttggc tggggttgaa cccgtaattt taaaggaagt
     5821 caggcccaag tctggcagtt cttgttcaat tttttccaac attcccttga actgagagaa
     5881 aatcaagaca cggtgtccgc cgtctgccac ctgtaccagt aggtctcgga gactatctag
     5941 tttgccgctg gctccctgat aatcttccat aaacagggca ggagtgtcac atatttgacg
     6001 caagcgcatc aaaccagata aaatttccac acgacttcgc tgaaattcct gttctgacac
     6061 ttgagccaga tggtctcgca tctgttgtaa ctgggcaagg taaatagcct tttgctggtc
     6121 ttccagttca tttttataaa tcacctcaat caagtctggc aattcagtca gaacttcttc
     6181 tttcttgcgt cgcatcacga aaggcttgat aaactgagcc actcgctctg ctggcaattt
     6241 cataaattct ttcttgcttg gcaaaagtcc gggcatgacg atttggaaaa tagaccacaa
     6301 ctcacccaga tggttttcaa tcggagttcc tgacaaggca aagaccgacg gcaccacaaa
     6361 ttgtctcaag gtctgggcaa tcttggtctg ggcatttttc atgacctgag cttcatctaa
     6421 gaaaaggaag tcaaaggcca tcccctgata aagctcactg tcctgacgga aggtggcata
     6481 gctagtcaca tagatttgat ggttatcggc aagaatctcc tcacgacttg ctttcaaacc
     6541 atgaacaaca gccacatcca actgtggagc aaatttctga aactcatccg cccagttgta
     6601 aatcaaaccc gacggagcga gaatcaagac tcgactttct tttgtcactt gactagtcaa
     6661 aaaagcaatg gtctgaaggg ttttcccaag tcccatatca tcagccaaaa tcccaccaaa
     6721 accataatga tggagcatct gcaaccagcc aattcccttt tcctgataat ctcgcaagtc
     6781 agtcttgacc tgagttgctt gcaaaggaaa gtcctctgga tgtgtcaaat cctgggccag
     6841 attctggaat tcttgtgaaa aagaaacacg gtctcgccct tcaaagagat gagctaaact
     6901 gtaggctaag gatttccgag cctgcaaggt cccatctttt aattcaaatt gccccagttc
     6961 ctgtagattt tggcgaattt tcttggtttc ttcatcgaaa aagtaaactt gattagacga
     7021 atcaatataa aaatcctgat tggcaaccaa ggcctgtatg gcttggtcga tttcctcctg
     7081 ggcaatatct tgaaaatcaa actggatttc caagagacct cccttggagg caatctgcac
     7141 ctgaggactc gctaggctat aaagctcttc tagtttatct gataggtcaa catgcccgag
     7201 tttttcaaag actggaatga tatcatgaaa aaaatgatag acagactccg cttttaaggc
     7261 ctgacgccaa gattgaaaat cggcttcaaa gcccgcagcc aaacagactt ggaaaattct
     7321 ttcttctaag tccgcgtcac ttgaaaatgg taattcttct agctcttgtc ggctagatac
     7381 ctgtctattt ccataatcaa actgaatttc taaacgaatc cgattatctt cttccctgtc
     7441 aaagtaaaaa gagggcgcaa aagttttgat ttgtagacgt tctggagctg aaacggtgcc
     7501 catctggata aaaagagtca gacaggaggc caatttgtct cgatcactgc tatcaaattg
     7561 caggtatttc tttccttgtc gatccacagg taacgcttta atttccttga gaagacgcat
     7621 ctgctggtct gttaaaaaat aaacctgacc tttatggaaa agtactgctc cttgataaaa
     7681 gacattgacc ctaggactct cactgatttc catttcaaaa tagtccgaat attctgttac
     7741 tgtaaaggca aatagattgg catcagcatg catatcctga aaaagcaggg tttggtagct
     7801 atccacttga tggtcaaatt gaaaatgggg caaggccatc agtaaattca caccctgctc
     7861 aaaaaaggtc agagggaaaa agaggtgccg accttggttt tggaaaaaga ggtctggaac
     7921 cagcccttcc tccgttagtc cgtgcaagaa agtcaaaagt tcttggctgg catcatcaaa
     7981 ggcttcccaa gaaagagact cctcataaat cttgccaatc atatacgact ttctctgctc
     8041 gacaatcctt aaaaaaagtg gaatatcccg aatgacatag tatttttggc tattgatttg
     8101 gccgattctc agagtccaca agatatgatt ggttcctgct tccacctgac ccacagctga
     8161 taactcatag gcgcattctg attttggaga taaaattcga tccaaaaact tgccacccaa
     8221 ggtcaccttg gtttcaacgg cctctttttc ttcatgacct tcttccagac tccacaagat
     8281 ttcctgacca cgctcatcat ttttcagaaa atgctctagc gctgccaaat gcacacagta
     8341 gcccctcttt tgaaaaaaat cacaggcaca aaaaaccaaa tcatcctcta aactatagcg
     8401 cagttcttct tctgcaacgc gagcgtagag ccgattgttc ttttccttga tgatatcaac
     8461 cttaccagtt tcataaaggg caacaccttc gatacgaatt ttccccggaa tcaatttagc
     8521 catattttca cctttacctt atctttttat tataccatat tttcgcctat gaaaatagcc
     8581 ttctaggaag acttttctcc tagaaggctg gatttttaa
//
LOCUS       DAVMMR010000071         7963 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMMR010000071 DAVMMR010000000
VERSION     DAVMMR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.071"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(340..636)
                     /locus_tag="VYS74_001789"
     CDS             complement(340..636)
                     /locus_tag="VYS74_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV3789786.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1167..2060)
                     /locus_tag="VYS74_001790"
     CDS             complement(1167..2060)
                     /locus_tag="VYS74_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789787.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2814..3044)
                     /gene="blpU"
                     /locus_tag="VYS74_001791"
     CDS             complement(2814..3044)
                     /gene="blpU"
                     /locus_tag="VYS74_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV3789788.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            complement(3247..3537)
                     /locus_tag="VYS74_001792"
                     /pseudo
     CDS             complement(3247..3537)
                     /locus_tag="VYS74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3583..3816)
                     /locus_tag="VYS74_001793"
     CDS             complement(3583..3816)
                     /locus_tag="VYS74_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3789789.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3822..4814)
                     /gene="plsX"
                     /locus_tag="VYS74_001794"
     CDS             complement(3822..4814)
                     /gene="plsX"
                     /locus_tag="VYS74_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV3789790.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4811..5581)
                     /gene="recO"
                     /locus_tag="VYS74_001795"
     CDS             complement(4811..5581)
                     /gene="recO"
                     /locus_tag="VYS74_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV3789791.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDDRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(5578..6747)
                     /locus_tag="VYS74_001796"
     CDS             complement(5578..6747)
                     /locus_tag="VYS74_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3789792.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6896..7906
                     /locus_tag="VYS74_001797"
     CDS             6896..7906
                     /locus_tag="VYS74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEV3789793.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
BASE COUNT         2346 a         1828 c         1262 g         2527 t
ORIGIN      
        1 atctagaaaa tacaaaataa ataaataaat gaatgattgt aaaatttgga aagaataata
       61 tttcttgaca atggcaagaa gatagagaaa aatatagttt ttccttatat agaaagtatt
      121 tattttttta ctttggatag gcagtttcat attataacga ttcaaagtag cgtaatctac
      181 taccaaaaaa agtaaaaata atttcccttg aaaaggaaga tagcttttaa aatagattga
      241 caaacttatg ataacaaaaa acaaaagggt tatcccaata aaattaagat aacctaaaat
      301 actttcacat tcttttttag aaagtatttt catctagttc taccatagag ctgctttagc
      361 agcacctttc tttttcaata tataaagaac tgttgctgca atacccgaac caatagaacc
      421 tactcctgtg attgcaccta caattgaaat tactgctgta actgtactat aagcactaat
      481 caaattgact acagtagcag cagcttttgt cgaaattcct aatgtcccag ccaacatagg
      541 aaaagaaaca acacctaagc ttaaccaact aatgactgct aacattgctg aaacaataag
      601 aaatttatta tgaaattcca ttcttttaga tttcatttta gtgtactcct tttgtatatt
      661 atgtatgttt tattgagagt taattcgcac atgttgtatt cctaactctc ttcaaatgtt
      721 cgtctatatt ttatactact tttattaaca tatctataat aaataaatta ctactacatt
      781 ttcctagatt atcaaattac ttttatatca ctaagactca ataaaagata atgtctattt
      841 aacttttata cgattactac cttcttttta gtttacactt cctacatcat aaataaacat
      901 actcaaccct cacaatctcc aaataactct tctatatcat tgaaaattga gtttcatcct
      961 ctatactaat gtaggacatt cctttaatcc aatataagaa taccttgttc cattaatctc
     1021 tacccgatat tatagataga accaaattcc tatacttaaa aggacaaata acgttccaac
     1081 cataataaac ctcctttctt tttgagaacg ttttctttat aggtttagta tagcaaaaac
     1141 atgaaaaaaa caacatgact tagaagtcag gttgttggga aatctatctg tttttctgct
     1201 aaaattctct gaactaatac ctgatcgtct agcaattctg ctagagaaat cgccttttca
     1261 tagaaacttt ctgcctctaa aatattcttg tctacaaata aagcatattt tgcctttagc
     1321 aaaaatagat gaggtcggta aaaaacatgt cttgttttag caataatttt ctcaaatgtt
     1381 tgaatatata tcagactatc ttctgtctga atttggataa ataggctcag aagaactttt
     1441 tccatttgaa ctaggctagc cttatcttca tattctgaac atagcaatac tttttgagct
     1501 aactcctcaa aatgttcttt atcttctaat cccatagcac aacaaaagaa atacaattca
     1561 ataatcaaca aatcatttac agaaaaatat ttctttttta ataactgctg gaaatattct
     1621 tccactaacc ctaaaccgta ggtcacatct gaactaccat aaatatcaaa acgagcttgt
     1681 gtcacatcca caattaactg ctcttcctca ggcaaattct cataatagtt ttcatatatt
     1741 tcctcaagta tagcttcttt tcgttcgatt cttcccttat cagcgtaggt ttgagaatga
     1801 atcagctcat ctttgagttt taggaatcct tttggaatct ctataacctt aacatccaca
     1861 atattctgta atgatactcc aagtttatcc gcaatcaata ataatttagg aagtgttgct
     1921 aaagcttgcc cattttcaat acgtgctagc tgacgaatcg tcaactctga ttcatcacca
     1981 caaatatcta tctgagtttt tccacaagat cgcctaatag ttttgatttt ataaccaatt
     2041 tcacgtttca actcatccat agtaatactc cttaagttga ttacaaagat ttctttttct
     2101 tcctaattct tttgtcaatc atatataaaa cgataagcga aaacagcggt agaacaatat
     2161 aactcttttc actcattatc agctgaaatc ccagggttgc tagtatctta aagatgaaaa
     2221 tattcatcaa aaatagaaaa acaaatacta aaaaatattt taaaaccttc ttcatttctt
     2281 ttcttcaact ttcctaattt ctacacctat tttatcgaaa atatatataa aactccaaat
     2341 aaacacttca actgcacttt ttttggcaaa aaatagacag aacaaacatc ctacctacta
     2401 aaaatatgat atgaaaggaa aatgattttt tactgacacc ctaggcaagc ttgggacaaa
     2461 taaatcattg atagcaacat ttacgacatc ctatttaaag ttccttcaat ttctaatgtc
     2521 cccgctattt ctaagccacc tctggtatga tctattccta gtcagaatag ataacaccgt
     2581 tctaaattac gattcatagt tactcctttt ttgactcgtt actataagac tcctatttac
     2641 atgcgcaaaa taattttgat tcagattcat catttatagt tttgattatt ttttaacaga
     2701 tttttctgtt ttcttctccg aagactttaa aaattttttt ctaattaaat catccatata
     2761 aggaccaaat ataccaacta ctaaaccaat aataaaactt ttaaaatcca taattaccac
     2821 caacatgtcg ctgcataggc cacacctcca agtatagctc ctcccacagc accagttgct
     2881 gcaccttgcc atgttcttgt tttaattcct agctgaagac ctcgtgctgc tcctcctcca
     2941 acacctgctt tggcaaaatc tccccaattg catccgccac cttcaacgca agcaagcatc
     3001 tcagtatcca taatttcaaa ttgtgacatc gtttttgtat tcataacgaa tacctctttt
     3061 ttatttttaa tatttgtctt gttacaaact tgacaagttt agtataacag tatctattaa
     3121 tttttttcat ccaaatcttg aattggcatc gaaacgtctt gaattagctt ttttgtttca
     3181 aaatcatctc tatttttaaa aaagatgttt tctaatcact tttttactat tagacttcct
     3241 gcaaaactag aatcctagtt catgattgat aataccagca atcaaattca ttcgtaatcc
     3301 gaagcgttta cgatgatttc gataggttgt tgaaaacatt ttaaacgttt ttactttggc
     3361 aaagatattc tcaaccttgc ttctctcctt agatagcgca tgattacagg ctttatcttc
     3421 aactgttagc ggcttgagtt tgctggattt acgtggagtt tgtgcttgag gatatatctt
     3481 catgagccct tgataaccac tgtcagccaa gatttttcca gcttgtccga tatttctcat
     3541 ttctaaaaac catctacttc cgttgacttg gaactccgat tgctattttc cttgaatgat
     3601 tttaaccaca tctcctacgt tttggagttg gtcaatttct tcatcgctga tttcgatact
     3661 aaattcatct tccagagtca agataaactc catcaagtca acagaatccg catccaaatc
     3721 gtctttcaga ctcaaggatt ctgtcacgac aaagtcctct ccctgtcgct cttggataat
     3781 ggtcacaata cggtcaaaaa tttctttttc tgtcatctct tttattctcc tgaaaattca
     3841 cgcgcagtct gggcaaccac gtctgtttct agcatggtac ggatctgacg aatcgtacta
     3901 taaacagcct tggcatcgct tgagccatga gtcttgacaa caggtgcctt aacaccaaac
     3961 aagaccgctc caccaacatc tgaataattg agctgttttt tcaaaccact gaggctgtcc
     4021 ttgagaagga gggcacctag tttcgctcga agaccaccac ctgtaatagc tgtcttgagc
     4081 aagcccatga ttcccatagc tgtcccttcg atggatttga gcacagcgtt tcccgtgaaa
     4141 ccatctgcca caacaacatc tgcaacgcca ttcatcaaat cacgcgcttc cacgtttccg
     4201 ataaagttca aactttcatc agccgccagt aattcataag tttccttacg aagcgggtcg
     4261 cccttgctac tctctgttcc gttgttgagc aaaccaacgc gtggttgcgc aatgccacgg
     4321 acatttttag catagaagga acctagaacc gcatattgat ggaggtgctg ggctgtattt
     4381 tctgcattgg caccaaggtc tagcatgtca aaaccttttc catcaacggt aggcaaggta
     4441 gacatgagtc caggacggtc gatattcttg atacgaccca cgatgaagaa tcctgctgcc
     4501 aacaaggcac ctgtattccc agccgaaagg acagcgtctg cttcaccatc tttgacagcc
     4561 ttggctgcca ataccatact ggcatttttc ttattccgaa tagctctcgt aggttcatca
     4621 tccgaatcaa tcttctcatc cgtatggata atgctgacgc gctctgtcgc tgtcagatat
     4681 tgcttgattt tagcttcatc tccgtaaagt tgaacctcga tatctgaaaa gtcagatagg
     4741 gcttgattga caccctcaac gatggcctga ggtgcgtaat cgccccccat ggcatctact
     4801 gcgatttttt tcatttcttt tcctctttta gtaattgtcc ccagtctgct agggaatcaa
     4861 taaatttctt tgattttagg tgaatcccaa cgtactcttc atatagttga tccataaatt
     4921 ggcgtagctc ttgcttgatt ccaggcttga gcgaaatggt ctccaaagtc tcaaaatcaa
     4981 tagcttgaaa ttgattgagc agatagggga tattgggatt gagatggcaa cgtctatcat
     5041 cctcatgata atgctctgga cagagacagg ctccatattt gaaagaaaag tcaaaagcct
     5101 gaccaacccg atggcagaag acacactcat taaaattgag gctgattcca aatcgagtca
     5161 aaatttgaat ttcaaaaata ttggtcaaaa cctgataatc caagcctgct tccatcaact
     5221 ccaaagtctt ttgcaaaaaa gcaaacaagg gagcatcctg ctgattgtcc tgcaaactag
     5281 catctgcaag agctgccaca taggtcgcat aggccatgac aaagaggtca ctattaatct
     5341 tgggaaaagt catgacctca tgatagtctt cgatgtaact gagtccgtca tcattgattc
     5401 gcaagagaaa tcgtgccagc accaagggct gaataacagg cgccagctta gactgaccag
     5461 cgtgtttgac aaaaaacatg cgtttgccaa cctgctctgt aaaaattttg acgagcttgt
     5521 catcctcacg aaaattgcga ttgtaaagca ccaagccttg actcgtgata gactgaatca
     5581 tgcttctctc atgtactcct caagtcgttt catggcttct ttgatagtct ccatgctggc
     5641 tgcataagat aggcggacgt agccttcccc gtaacgtcca aaggctgcac cagggataaa
     5701 ggcaacggcc ttcttctgag caaaatcctt cagaaaagca aaggagtctt gattgtagcc
     5761 cgctggaatt ttagcaaaaa tatagaaggc accgtctggt ttgataatct caaaaccaag
     5821 agcagtcatt ttttcgatga tatagtcccg acgctggata tattccttct tcatgggctc
     5881 cgcatcgttt ttaccagccg tcaaggcttc tactgcagca tgttgcgcca tggtatttgc
     5941 ggcagtgacc aagtactggt gactcttgat taactgggct gtgaaggtcg caggagcgaa
     6001 aatcagcccc aaacgccaac ctgtcatggc atgcgattta gacaaaccat tgataataat
     6061 agcctggtct ctcaacatcg ttcctagaga cacatgggct tcgcctgtgt aggtcaattc
     6121 tgagtaaacc tcatcacaga caacaaaaat ttcgtacttg cgtaaaacag ctgccaaggc
     6181 ctctaactgc tctcgactgt aggtaattcc tgtcggattg gctggatagt tgagaataac
     6241 cgccttgagc ttatcaccct gctccaaaat ggccttctcc aacatctcag gagtcaagac
     6301 aaaaccattt tcagtcgtat caatctcaac aatttctgcc ccaactaagt taacaatcgg
     6361 ttcatagcct ggataagcag gagctggcaa aagtaccttg tctccctctt ccaaaatagc
     6421 cgtcaaagtc gcagataaag cctctgtcgc cccaattgta accaagattt cattttcagg
     6481 agcatagtcc agttggtact tttccttaac aaagtcactg gctgcctgac gtagagtcag
     6541 cagaccactc atccctgtat agtaggattg gttctgatca atcgctcgct tggccgcctc
     6601 cttgacatgg tctggcgttg taaaatcagg ttcccccaag gtcaaacgca agaccccagg
     6661 aatctccgaa ataacctggt caaactgacg aatcaacgaa acttgaattt tatctaactg
     6721 tttattaaag cgcttagtta agtccataga tacctcctac tttcaaaacg tatctctatt
     6781 atactacaaa agccccccga ccgcaaaaat acattagaga atagctttcc actttctatt
     6841 ttacttttcc tgtttatagg tctataatgg taatagattt tatttggagg tttttatgtc
     6901 atttctatca aaaaatggag caggtatctt ggcctgcctt ctcatttcca tcctatcttg
     6961 gtacttagga ggattcttcc ctgtgattgg cgcgcccgtt tttgccattt tcataggcat
     7021 gctcctacat ccctttctct cgtcctataa acaactggat gctggtttga cctttagttc
     7081 caagaagttg ctccaatatg ccgttgtctt gcttggtttt ggtctcaata tctcgcaggt
     7141 cttcgcagtt ggccaatctt cactccctgt catcctgtcc actatctcaa tagctctgat
     7201 tattgcctac ctcttccagc gtttctttgc actggataca aaactggcta ccttggttgg
     7261 agtaggttct tctatctgtg ggggttctgc cattgcagcg acagcgcctg ttatccatgc
     7321 taaggaaaag gaagtagccc aagccatttc cgttatcttt ttcttcaatg tcttggctgc
     7381 gctcatcttt ccaaccctcg gcacctggct tcatctatcc aatgaaggct tcgccctctt
     7441 tgcagggact gcggtcaacg acacttcctc tgtaacggct gccgccagcg cttgggacag
     7501 tctttaccaa agcaataccc tcgagtctgc aaccattgtt aaactcacac gtactttggc
     7561 cattatccct atcacgctct ttctatccta ctggcaaagt cgccaacaag aaaacaagca
     7621 aagcctacaa ctgaaaaaag tcttcccact ttttatcctt tactttatcc ttgcctctct
     7681 cctcactaca ctactcacct ctctaggtgt gtccagtagt ttctttaccc ctctcaaaca
     7741 actctctaaa ttccttattg tcatggccat gagtgctatc ggtctcaaaa ccaatctggt
     7801 cgctatggtc aaatccagtg gaaaatccat tgttcttgga gccatctgct ggattgccat
     7861 catcctcacc actcttggta tgcagaccct tatcggcatt ttctaacact cttcgaaaat
     7921 ctcttcaaac cacgtcaacg tcgccttgcc gtaggtatat gtt
//
LOCUS       DAVMMR010000072         7628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMMR010000072 DAVMMR010000000
VERSION     DAVMMR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.072"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..282)
                     /locus_tag="VYS74_001798"
     CDS             complement(<1..282)
                     /locus_tag="VYS74_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789794.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VYS74_001799"
     CDS             complement(275..916)
                     /locus_tag="VYS74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789795.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VYS74_001800"
     CDS             complement(918..2948)
                     /locus_tag="VYS74_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3789796.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3025..3321)
                     /locus_tag="VYS74_001801"
     CDS             complement(3025..3321)
                     /locus_tag="VYS74_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV3789797.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(3641..4462)
                     /locus_tag="VYS74_001802"
     CDS             complement(3641..4462)
                     /locus_tag="VYS74_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3789798.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLDTS
                     RTRRPEYAELILEEHMSQIIEKEVYSTNDMLLIRLFFYQMLIRKDLAKFINQIEKLML
                     FLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDCFNDYLSCLQEIMDKTQDYQKKP
                     LVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4534..5094)
                     /gene="rnmV"
                     /locus_tag="VYS74_001803"
     CDS             complement(4534..5094)
                     /gene="rnmV"
                     /locus_tag="VYS74_001803"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV3789799.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(5094..5867)
                     /locus_tag="VYS74_001804"
     CDS             complement(5094..5867)
                     /locus_tag="VYS74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV3789800.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(6172..7065)
                     /locus_tag="VYS74_001805"
     CDS             complement(6172..7065)
                     /locus_tag="VYS74_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV3789801.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVGGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(7349..7483)
                     /gene="rpmH"
                     /locus_tag="VYS74_001806"
     CDS             complement(7349..7483)
                     /gene="rpmH"
                     /locus_tag="VYS74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV3789802.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT         2166 a         1800 c         1297 g         2365 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtctg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcaatactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagatga ttggtcatat tttccatgat gaggatatct tctaaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gtcttagcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 cgaaattttg aaagagatag cccatctggt ctcgaaaata ctcacgagta ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc caccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttatcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagtt cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc cacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagcctta cctgtgggag gtagtgtttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatcct
     1621 tggtagtagg cagactgctc tcgcaagctc tcaggcaaga tcagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgcggcg agacatagat aacattccct aacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc atctgagaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccaaggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgtaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taagctgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgataacca agctctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa agcctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accaagtaaa
     3001 gctaaacaat agggctatac cattctaaac attatagtag aatgcaactt tctctcccca
     3061 agaagtacga atccatgcac gagaagtccc tccagcacca gcatatccct tgtcagatga
     3121 accagttttt ctgctaacaa ctgttgaact atggtatttt gaagggtgat agtagtttga
     3181 gtaagcacca ccgttacctg aaccataagt ccaaacacca ccttctgggt attgaacggc
     3241 tgcagaggca actgatacac ctccacctaa tgttgctgca atgctcgcta caagagctaa
     3301 ctttttaact gtttgtttca tattgatact cctctatttt tgattacaag attgttgagt
     3361 tacttgctcc tctttccatt atcaacgttg atactattat attaccaaat gatattctaa
     3421 aattatttgt ttttctctaa attgtcaaaa tagcaccctg aaatgaactt ttatagtcat
     3481 tatactccat ctttatcaca ctatcaagga catcaatgtc cttaaaatct aactatttca
     3541 tattattttt agcttatatc taaaaaaaca gttctaaaga cgaactttag aactgttttg
     3601 atacgaggac aaggacattt ttgtcactga ttcactatgt ttataaatat tttttgacat
     3661 cctcttgcca ttcctctgtc aatttcttta ctagaaatcc atctccaatt agcttcgcaa
     3721 atgttttaga agactgataa aatgattcag ctaaactaaa atctctttct actcttaatg
     3781 cttgcttcca caaaaacata aatacaagag gtttcttttg ataatcttga gttttatcca
     3841 taatttcttg taaacacgat agataatcat taaaacaatc agttactcct accttttcaa
     3901 gacaacacat tcctgaaata agagtatctc taattataaa gaagttttct aatttagtta
     3961 cctttttctg ttccaaaaga aagagcatta gcttttcgat ttgatttata aatttggcaa
     4021 gatcttttct aatgagcatt tgataaaaaa acaaacgaat caacaacata tcatttgttg
     4081 aataaacttc tttttctatg atctgagaca tatgttcctc aagtattaac tctgcatatt
     4141 caggccttct agttctagaa gtatccaaac acgcttcttt cttatcatac tcctcttctt
     4201 taccatagat tggttcacgt aaaatctggt atttcaattc taaataagca gaaggaagag
     4261 ctgaaaaatc cggcataagg ctataaactg gaactcctag ccggcgagca atataggcta
     4321 acttgggcaa actcggttgc gaagctccac tttcaattcg cgacagttga cgaactgtca
     4381 gttcttgctc atctccacaa aaatctggac gactaattcc tttttctatc cgaatctctt
     4441 taattttttg accaattaac atcagacgcc tcctcttcta cctgcttcga aaaatactgt
     4501 gaaacttaaa aaattgtaac ggctacatct ttcctactca taagatttca tagcttcttc
     4561 cacttctgcc aaagtaaccc caaacaactc taggcgtttg aggagttgct tgccattaga
     4621 atagccgatt cggagagtct ctccgagata ttctctacgc ttacggctgt ctgcccctgc
     4681 tagaaaacca aggcgaatca agtcgctacg actaatgtca aactgactct catgttcaaa
     4741 ttgttctgtc acctgagcta gagccgtttt caggtcttca tagctggcat gctcaattcc
     4801 cagagaacgc cccttggtct tggacttggg aacagcttca tctcgcttga gaaaggcatg
     4861 ctgaactgtt ggaatggccg tcataatcat gcgccgaatc cgctccccat taaaatctgg
     4921 gtctgtaaag acaatgactc catgacgttg gtgtagacgc tgaattcgct ctatatcctg
     4981 atcattgatg gcagaacctc gtgtctcata ggtctccaca tcgaaataac gcttgagatt
     5041 gaccgtatca tagcgacctt caaccacgat aacttgagaa attttctctt tcattacttg
     5101 ctgtccaatc caaaaattcg ttctgcattt gcagtcgttg ctaccgccag ctcttctgtc
     5161 gtcataccac gcaagtcagc gataaagtcg accacatagc gagtataggc tgttttattt
     5221 tcacgaccac gcttgggtac aggtgctaag taaggcgcat ctgtttccac caacatcttg
     5281 tccaaaggta actctttagc tgcttcttgg aggtcagttg ccttcttgaa agtcaccact
     5341 cctgagaagg aaatggtcat accaagatcc acaaacttct ctgcccactc aagcgtccct
     5401 gaaaatgaat gcatgatacc accacgagga ccaacgccct cactcttgat aatctcatag
     5461 gtatcttcca gcgcatcacg ggtatggaca acaaaaggca aatccaagtc cttagatagc
     5521 tgaatctgac ggcgaaaaac ctgctcctgc acctctttgg gcgctgtcat ccaatggtag
     5581 tctaagccaa tttcacctaa agccacaacc ttggaatgtt ttaacttatc caacaagtaa
     5641 gcctcaactt cctctgtata agtaccagct tctgtaggat gccaaccaat agtcgcatag
     5701 agctgctcat actcatctac caactccaag gcacgctcaa tcgtcggttt atcaaaacca
     5761 acaatattca tctgtatcac acccatctca gcagccaagg caatttcttc tgcctcacga
     5821 cctgcaaatt cttctacatt caagtgtgta tgtgtatcaa aaatcatctc ttctaacctc
     5881 gttttctatc ttctattata ccaaaaaaga ggaggggcac ctaatttttc ggtttcccct
     5941 cctctcttca atagagagct attctgctat cttttctatc cgatattgcc catctcctat
     6001 tccacagtta gagacagaag agattggcta cattgataga atattcctat tcagctaaag
     6061 tatgtgatta tcttgggttc tcacttttct cacccatcac taggattgat gacttctcaa
     6121 aatccccctt ataatctaac tttaagtaga gaatagaatt ttctagctgt tctattttct
     6181 acgtctagtc tttcttccta gcaaagcaaa accacttgtt gccaccacta gaccaagact
     6241 agcaagtgct gcacttgatg ctgaacctgt gttaggtaat tgtttgccag caggtgactg
     6301 gtaggtaaca tcttgcattg ttcctgcagt tgcttgcggt gcacttgttg tcttaggagc
     6361 agcattagga gccactggtc tcttaggaac tgtaaccatg gcaatggctt gcactccaat
     6421 cgtttctgct tccttcacat cttccatagt tttcgcattt tcaatctctt tgagagctgc
     6481 ttgtttttct gcttccactc ttgctaaaag ttctgctttt tctttatcag aaagcggtgc
     6541 gcctttgatt tcatcctgct tatccttggc tgctttttca atggcatttt tagctgattc
     6601 tttttcagta gctaattgct tagaagcttg tgattgagct gagttttgtg aacctgtgtg
     6661 agctgatcca cccgcctggc taccaccgcc ttcattcgca ttagcatctg agcttccagt
     6721 attgcctccc ttatctccac ctggtatagt ttctagctta tactctagtg tagcttcttg
     6781 agttacagat tcacctttcg cagattgttg aactgtatct tttcctagaa tcgtcactac
     6841 tgaaccatct gggaatgtga ttgttgctgt accatctcca cctacattga ttgtcgctcc
     6901 gtctaatgct gaaccatttg cttgtaaaat agcaacctta actttttctt catctgttaa
     6961 atgagttata tcttttacat ctactctttg agcaggtgct ttaacagttg atttttcagc
     7021 agtcggttta gtgctatctt gtgcttttgg atcttgtgct accaattgat ttcctggaat
     7081 tgtaatagct gaaccatctt taaagacgat tgttgctgta ccatctccac ctacttcaac
     7141 tttatagtaa aataaaataa taacaggaca aatcgatcag gacagtcaaa tcgatttcta
     7201 acaatgtttt agaaacagag gtgtactatt ctagtttcaa tctactataa catcgcttcc
     7261 gaatccattt gcatctactg aactttttta caaaaataaa aatcagtaga ctcgagttcc
     7321 tactgatttt tgatagaatt cgtttggatt atgcagccaa aactttgcgt cctttacgac
     7381 gacgagctgc caatacgcga cgaccatttt tagttgacat acggttacgg aatccgtgtt
     7441 tacgcgcacg acgaagttta cttggttgat aagtacgttt cacgatgaat acctcctcat
     7501 agattttgta ttcgtttagc cggctataaa gtgatcagtt actaaacata ctttactatt
     7561 ctatctgatt ctttcacttt tgtcaatagc tctaggcaaa tgtttccagc tttttcgaat
     7621 gcaagccc
//
LOCUS       DAVMMR010000073         7473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMMR010000073 DAVMMR010000000
VERSION     DAVMMR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.073"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            122..277
                     /locus_tag="VYS74_001807"
                     /pseudo
     CDS             122..277
                     /locus_tag="VYS74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VYS74_001808"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VYS74_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV3789803.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VYS74_001809"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VYS74_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV3789804.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2277 a         1575 c         1590 g         2031 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagaa
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgctt tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggtttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cag
//
LOCUS       DAVMMR010000074         7230 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMMR010000074 DAVMMR010000000
VERSION     DAVMMR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.074"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            99..1409
                     /locus_tag="VYS74_001810"
     CDS             99..1409
                     /locus_tag="VYS74_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV3789805.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            complement(1775..1969)
                     /locus_tag="VYS74_001811"
     CDS             complement(1775..1969)
                     /locus_tag="VYS74_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3789806.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(2428..3156)
                     /locus_tag="VYS74_001812"
     CDS             complement(2428..3156)
                     /locus_tag="VYS74_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3789807.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            3314..4723
                     /locus_tag="VYS74_001813"
     CDS             3314..4723
                     /locus_tag="VYS74_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3789808.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4741..6036
                     /locus_tag="VYS74_001814"
     CDS             4741..6036
                     /locus_tag="VYS74_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3789809.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            6041..6349
                     /locus_tag="VYS74_001815"
     CDS             6041..6349
                     /locus_tag="VYS74_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3789810.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            6346..6654
                     /locus_tag="VYS74_001816"
     CDS             6346..6654
                     /locus_tag="VYS74_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3789811.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         2351 a         1326 c         1267 g         2286 t
ORIGIN      
        1 ttttgtgtat aatagtcaag taccaatagg aaacaggtgg tcttttgagc gccaaaattg
       61 ccaacctatt tccatattct aactgaaagg acaattcaat gattcattta ataatgatta
      121 gcgccattgc tctagccatt ggaattggtt accgcaccaa aatcaatatt ggcctgctgg
      181 ctattgcttt ttcttacctc atcgcaacca ctctcatggg attaagcccc aaagaacttc
      241 ttcatttttg gccaacctca ctctttttta ccatttttag cgtctctctc ttttataacg
      301 ttgcaacaac taacggtact cttgatgttt tggctcaaca cattctctac cgcacacgca
      361 cccaccctaa cgccctctac atgattttat acctgatggc aacccttttg tctgctttag
      421 gtgctggatt tttcactact atggccgttt gctgtcctct agcgattacc ctctgtcaaa
      481 aagcggacaa acaccctttg attggagctc aagccgtcaa ttggggagct tcaggagggg
      541 ctaatttgat aaccagtagc tcaggcattg tctttcaagg cctgtttaag caaatgggat
      601 gggaagagca agctttttca cttggcaatc atatctttat cgtcagcatt atctacccac
      661 tcatcgtctt gcttttatta tcctgttaca gtcactatag taagggaaga acaaactcat
      721 ccttgactat tgaccaacca ccactcctca gcaaggttca aagacaaacc accttgctca
      781 tgatcagcag catggttttg gtttggcttt ttcccttgtt gcatctcatt ttcccaaaca
      841 tcgcctggat agctacttat caaaaaacat ttgatattgg ctttgtctca atactcatgg
      901 tctgcctcgc cttaaggctc aaattaggga agcaagaagc cattttagct aaggttccct
      961 gggctacaat tatcatgctc tgcggcatga gcttactaat gtctctagcg gtcaagtcag
     1021 gtttagtcac actgataggg cacctcatga caacaaccat tcctcatttc tggttacctc
     1081 tgttcttttg tgtgatagcc ggagtgatgt cactctttag ttccacccta tccgttgtag
     1141 ctccagccct ctttcccatc attgctatca tcagtgctca aaatcctcag attgatattc
     1201 acttactcac aacagccacc gttattggcg ctctatctac taatatctca cccttttcat
     1261 cagctggttc tctcattcaa ctctcacttc ccaatataga ggaacgtggc cttgccttca
     1321 aaaagcaaat tattctgggt gtccccatca gcttaagttt gggactcttg accacctgga
     1381 ttctcatcct tctagcttcc ttaagttaga tttattatct aacttaagag tggacttggt
     1441 ataataaagt agacacaaaa ttaagtggaa acgaggaaaa gtcattatgc caagaaaaac
     1501 ctttgataaa gccttcaaac tctctgctgt aaatctcatc ctgtaaaaat gattagttca
     1561 actttaaaaa ttcatcccaa tagtctttat cgatggattc aagaatatga aaaatatgaa
     1621 gaaagtgcgt tcccaggaca tgggagcgca cttcgtcata ctcaatttga gatatagtga
     1681 aatgaaataa gaacaggaca aatagatcag gatagtcaaa tcgatttcta acaatatttt
     1741 agaagtagat gtgtactatt ctagtttcaa tctactatag taaagttgac tttcctgtac
     1801 catttttacc agtaattaat gtcacgtctg aactcgaagt atcaaaagaa atattatcaa
     1861 acaatttacg atgatccacc ctatacgaaa tattttctac tttcattttt tctccttttg
     1921 aactaatagc aaaagaaaca gttctgtttt catactcttt ccccttcatc tatattattc
     1981 gtaatgaaaa ataaaaaata aaattttttt aaatgattat gatttagaat ttatttaata
     2041 tattaaaaga agatatgaac aaataaattg ggagattgaa attcatttct agcaatttgt
     2101 tagaagtcac agtctactat tctaaccaca gtatactata tgtgttacaa gaaaagactc
     2161 aaaaacttct cgtcagtgta agaatgtttg aaacactatt taaaactaaa taactataag
     2221 aagtagatca ggtgaacaat ttcagtacct actctataag ctatacgaag atatttttca
     2281 acatgatgga tacaacgatc actcttttaa agccatctta tttagaaaag agaaaccatt
     2341 taagcataga tgggaccttc ttgccctgtt ttaaattttt cttaaactag agtctgtaaa
     2401 agattggctt cattttcaca ttttctccta ggaggaatgt cgtaatgcaa aagaattaaa
     2461 ttcaaataaa tcatagcgat gacgactaat cgagtactca aacggagttc cgttatccaa
     2521 ataagccact tgttctactt caaataccgg ttccgttgga aggagctgta agtaatgttg
     2581 ctcattttct gaactagaag aagctcgcaa gatttttgta gcactggcaa tatgcaatcc
     2641 taactttcct tcaatgtaat tgtaaatcga tttttgtaaa atatcttcag taatacctgg
     2701 aataacatcg gtactcatat aagtttgttc cagaacataa ggtttaccgt caataatacg
     2761 caggcggtaa atattataaa ctggactctg caaagcgacc tgtagttttt ctgctaaaaa
     2821 ttcatttgca aattctaatt tgaagtgaat aatcctcgag tcaattgtag aatgagcatc
     2881 ttcagatatt tttgtcaatc cccggatatc tctttctgga acgattaatt ttctttttga
     2941 gctgccacga gagagaacaa aggttccctg cccacgtttt ctaaaaatta agccctcaga
     3001 aactaacaag tctaaagctt ttttgatcgt cattcgacta caggcaaact gtttagctaa
     3061 tgtcatttca tcaggcaatt gttcgttcat ggtatattta ttacaaagaa tcttttcacg
     3121 aatctcattg taaataaata aatattttgt actcatttaa tctggttctc tctataatat
     3181 tctaatagta gtataacaaa gttcaagaaa tgaggcaaac acaagatatg tatcaacaat
     3241 ttttaaaaat gtctagacaa attttaaaat tatgctatac tttatgtata gaataataga
     3301 aaggaggttg agcatgaaaa aaaagttagt atttcctaat ctgttttggt ggggagctgc
     3361 ttctagcgga cctcagacag aaggtcaata tggaaaagta catgaaaatg tgatggacta
     3421 ctggttcaaa acgcatccag aagatttttt cgataatgtc ggacctcttg tagccagtaa
     3481 cttttttcat acttacaccg aagatttcca cttgatgaag gaaattggag ttaattcttt
     3541 ccgcacttcc atccaatgga gtcgactcat caagaattta gagacaggtg agcctgatcc
     3601 aaaaggtatt gctttctaca atgccatcat tgaagaagct aaaaagaacc agatggatct
     3661 tgtgatgaat ttacatcatt ttgatttacc agtggaactt cttcaaaaat acggtggttg
     3721 ggaaagcaaa catgtagtgg agttattcgt gaagtttgcc aagactgctt tcacatgctt
     3781 tggagataag gttcattact ggacaacttt caatgagcca atggtcattc cagaagcagg
     3841 atacttatat gctttccatt atccaaatct aaaaggaaag ggaaaagagg ccgtacaagt
     3901 catctataat ctaaaccttg ctagtgcaaa agtgattcaa ctatatcgct cattaggact
     3961 tgatggaaag attgggatta ttttaaactt gacacctgct tatccaagaa gtaattctcc
     4021 agaagactta gaagcaagtc gatttacaga tgacttcttt aacaaagtct tcttgaatcc
     4081 agctgttaaa ggaactttcc cagaaagatt ggtaaaacag ctagagagag atggcgtgtt
     4141 atggagtcat accgaaaaag agcttcaact gatgaaatca aatacggttg attttcttgg
     4201 agtaaactac taccatccaa aacgtgttca agcacaagca aatcctgagg aatatcagac
     4261 gccctggatg ccagaccaat acttcaaaga gtatgaatgg ccggagcgtc gcatgaatcc
     4321 atatcgtggt tgggaaattt ttccgaaagc catttatgat attgctatga ttgtgaagaa
     4381 agaatatggt aatattccat ggtttatcag tgaaaacgga atgggtgttg aaaacgaagc
     4441 acggtttatc ggtgaaaatg gagttatcga tgacgtgtat cgtattgaat tttatgaaga
     4501 acatttaaga tggctacata aagccattga agagggaagt cactgttttg gataccacgc
     4561 ttggaccgca tttgattgct ggtcttggaa taatgcatat aagaatcgtt acggatttat
     4621 ctccgttgat ttagaaacgc aaaagagaac catcaagaga tcaggaagat ggtatcgcaa
     4681 agtaagtgac aataacggtt ttgaagtaga aattgaggag taaagagagg agatattcag
     4741 atgaaaaact caaaatttat agatcaattt gccacctttg ctggtaaact agggaaccaa
     4801 attcatttaa aaaccctaag agatgcattc gtaacagtaa tgccattata tattttggca
     4861 ggtttaatcg ttcttttgaa caacacggta tttaagtgga ttttccaagg ggatacatta
     4921 acaagattcc aatattgggg aataacaatt gcaaacggta ctttaagtat ttcaggtatg
     4981 attattgctg taatggttgg ttatttctta gctaaaaaca gagatttcga aaacccgtta
     5041 gcagcatcaa tgctatcatt agtttcttta attgtgatga tgccaaatac agtttctgta
     5101 gttcctgacg gagcaaaaga tgcggtaaac atttcaggtg ttctttcatt caacaacaca
     5161 ggtacaggcg caatgttcgc cggggttatc gtagcgatta ttgcaacaga attattcatt
     5221 gaattatcaa acgttaaagc tttacaaatg aaccttggtg aaaatattcc accagctgtt
     5281 agtagatcat ttagcgtatt acttccagtc atgaccgtca tctccttatt tggggttgtt
     5341 tcagcattat tattcaatat aactggaatg aacttaatct caatcattac aatctttatt
     5401 caagaaccaa ttcgtcatat tggtacaagc ttaatcgggg tcattattat ttactcttta
     5461 ggaaatatgt tatggctatt tggtattcac caagcagtta tttacagtgc catcctagaa
     5521 ccattactat taattaacat tactgaaaac atcactgcag caaataatgg acaagccatt
     5581 ccacacatca tcaacctatc acaaatacaa acattcgctt taatgggtgg tagtggatct
     5641 acattatgtt tattaatagc aacattctta gtgagtcgca atgctgtctc taaaaacgtg
     5701 gctaaattat cttttggacc tggtatcttc aatatcaatg aaccagtatt attcggttac
     5761 ccaatcgttt ataacatttc attagctatt ccatttatca cagttccagt ccttggtatt
     5821 ttaatcagct acttagcaac agttacagga ttcatgagtc ctgcatttat acaagttcct
     5881 tggactacac cagtattctt aaatgcatgg ttagcaacag caggggacgt gagagcagtt
     5941 ctagttcaat tcatcatctt tgcacttgga gttcttctat acattccatt tatcaaagtt
     6001 aatgacaaag ttgttgaaca agaaatggaa ggttaagatt atgaaacaaa ttttattagt
     6061 atgtaatgcg ggaatgtcga caagtatgct tgttaaaaaa atgcaacaaa gtgcgactga
     6121 acgtgggatt gaaatttcta tccaagcaaa atcaatgaca gaagctaaaa agaatatcca
     6181 tgaagcagat gtcattctaa ttgggccaca aattcgttat gagttacttg ctgtgaaaga
     6241 aattgcagga aatatccctg tagataccat tgatatgcgg gattacggaa tgatgaatgg
     6301 agcaaaagtt ctggaacaag cacttgcatg gataggagaa ataagatgac agaagaaatg
     6361 gaatatatct gttttcagct cattgccaat agtggagcag ccaaatcatc ctttattgaa
     6421 gccatccaac tagctaaggc aggaaattta aaagaagcga aaattaaagt tgaagaagcg
     6481 gaagactcct tagtagaagc gcacaagatt cactctaatc tcatacaaaa agaagctact
     6541 ggagaaaaaa taggattctc cttgctattt atgcatgcag aagaccaaat ggcttcaaca
     6601 gagattattc agttgctatc taaagaattc atcgatctct atcaaaataa ataggaacac
     6661 tgtcattccc atctgtcttt attaatgacg gctacattgt aggaacctcc aaaagttgga
     6721 caaaatgatt gaaaggattg gatcctgcat tacacaagat ccaatccttt tcttttactc
     6781 tagtttttat aatgtgttga atttagaata gtccactgca gtttctaaaa aattgctgga
     6841 aatagatgtt aagctctaat ttatttgttt atatcttatt tcagtttact atactagctt
     6901 attattctag taaaaaattg aatgagccac tatttattta atatgtatga tgagggaatg
     6961 actcaaaaac ttcttgtcga tataagaata tctgagatag tattttaaaa cgaaaggatt
     7021 ataaaagctc atttccttcg cacttatttt atagagatct atcattatac tctttagaaa
     7081 tcaaaaagca acacaccttg tcatcgtttt ctcgtttgct ctttgatttt aattgggcct
     7141 tagtaaatac tactcttaaa ttaaactata gtgaattgaa ataagatgta aacaactcta
     7201 tcaggaaagt aaaattaatt tctataaatg
//
LOCUS       DAVMMR010000075         7228 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMMR010000075 DAVMMR010000000
VERSION     DAVMMR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.075"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(86..1048)
                     /gene="nrdF"
                     /locus_tag="VYS74_001817"
     CDS             complement(86..1048)
                     /gene="nrdF"
                     /locus_tag="VYS74_001817"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV3789812.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1236..3395)
                     /gene="nrdE"
                     /locus_tag="VYS74_001818"
     CDS             complement(1236..3395)
                     /gene="nrdE"
                     /locus_tag="VYS74_001818"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV3789813.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMKDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3476..3694)
                     /gene="nrdH"
                     /locus_tag="VYS74_001819"
     CDS             complement(3476..3694)
                     /gene="nrdH"
                     /locus_tag="VYS74_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV3789814.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            4045..4308
                     /locus_tag="VYS74_001820"
     CDS             4045..4308
                     /locus_tag="VYS74_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV3789815.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4314..6047
                     /gene="ptsP"
                     /locus_tag="VYS74_001821"
     CDS             4314..6047
                     /gene="ptsP"
                     /locus_tag="VYS74_001821"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV3789816.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            6302..>7228
                     /gene="phtA"
                     /locus_tag="VYS74_001822"
     CDS             6302..>7228
                     /gene="phtA"
                     /locus_tag="VYS74_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV3789817.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGL"
BASE COUNT         2233 a         1348 c         1552 g         2095 t
ORIGIN      
        1 gagcaaacta ggaagctagc cgcaggctgt acttgagtac ggcaaggcga agctgacgtg
       61 gtttgaattt gattttcgaa gagtattagt ccaaaccgta gttgtagtca tcatcttgca
      121 tggcttcaac ttcgccaaga aggtaaccat ttccgacttg agagaagaag tcatggttgg
      181 aagttcctgt tgaaataccg ttcataacga ttgggttgac atcttcagct gaatctggga
      241 aaagtggatc ttgtcccatg ttcatgagag ctttattggc attgtagcga aggaaggttt
      301 taacctcttc agtccaacca acaccgtcat aaagactctc tgtgtagcct tcttcatttt
      361 cataaagagt atagagtagg tcgtacatcc attctttgag tttttcttgc tcttcttcag
      421 gtaattcatt gaaaccaagt tggaatttgt aaccaatgta ggttccgtga acagactcgt
      481 cacgaataat caatttaatg atttctgcaa cgttggcaag tttgttgtta ccgagatagt
      541 agaggggagt gaagaaacca gagtagaaga ggaaggtttc gaggaagacg ctggcaactt
      601 tcttttcaag tgggctgccg tttaggtaga tttcgttgac aatctcagcc ttcttttgta
      661 ggtaaggatt ggtattggtc cattcgaaaa tttcttcaat ctcagcctta gtattcaagg
      721 tagaaaagat tgatgagtaa gatttagcgt ggacagattc cataaattgg atgttattga
      781 aaacagcttc ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc ccagtttcag
      841 attgcatagt gtcaagaagg gttaaaccac caaaaacttt tccgaccaag tctttctctt
      901 tgttagatag ctttctccag tcatccaagt cgtttgataa gggaatacgt gtatcgagcc
      961 aaaattgctc cgtcagtttt tcccaagttg atttgtcgat gacatcttcg atggcattcc
     1021 agttaatggc tttgtagtaa gtttccattt aaaatctctt tctgtgttta gtattgcgaa
     1081 ctcacaatta tttctacttt accataattc tataggagta tcgcacaaaa agtcggaagc
     1141 ccgactttta aaatgttaca taaattatgt tatgacatag tagatttgat tttatcagtg
     1201 ctgcttaggg aaaaatagtg tttctatgct agaaactaaa tcacacagct ttcacattgg
     1261 ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat agacttgatt
     1321 cccttgttaa aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg tttattttcc
     1381 ctcttccatt cgtaaaggcc ttttggaatg tcactacgca tgaagagggt gagtgaaagt
     1441 ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg catatccata
     1501 tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc agggtagtag
     1561 attttaccaa ttttcttttc ctgacgttct tcaatacgtt gcgtaatcgg gtggatagaa
     1621 gcagaaacat cgttgatgta gctgatagaa ccatttggcg ctacagcaag gcgattttgg
     1681 tggtaaagac catcctcttg aaccttgtcg cgaagttcag tccaatcagc aacaccaggg
     1741 ataaaaacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa ttcaccagtt
     1801 acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt gtggaaggta
     1861 ataccacgtt cacgcgcaat attgtttgac tctaccaagg tccagtagtt cataagcata
     1921 aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag ttgttgggca
     1981 aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct atttccatgg
     2041 tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag ggcacgaacc
     2101 atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac gttggttgaa
     2161 cccaggttac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc gttgatcaag
     2221 cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat ctttccatcg
     2281 acaggatttg cacggttagc cgtatctatg ttgactacat aaggatagcc agactcttgt
     2341 tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt gcgaatattt
     2401 ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa tggcacaccg
     2461 tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt acgagccaat
     2521 tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg tactttttca
     2581 tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa gacgttgagg
     2641 tagacaacac cggcaccttg acgttgcccc aattggttgg agtaagagaa gctgtcttca
     2701 aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt gataggtgca
     2761 ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg tgaaagttga
     2821 agagctgagt tgatagaacg tccgatagag ttcatatcat ccgtcacttg gattaggaaa
     2881 caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg agtagcaggt
     2941 tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc attcccatca
     3001 gcgaaataaa gggcgttgaa gaagacacgg tctttcatat tttcaagata gtattcaccg
     3061 tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa tgacttgaat
     3121 tggaagtttt ggtctttgat aaattgagcc aattcttcca agaactctgg acggtatttc
     3181 ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt gtctttgatt
     3241 gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc caaggcttcc
     3301 ttatctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt attaagacgg
     3361 aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc taattacaaa
     3421 agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac taagattatg
     3481 ctaattgttt cagtttttct ggttggaaac ctgaaaagac ttcagttggt gtttggataa
     3541 caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt tgctcatcaa
     3601 gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc attttacatt
     3661 ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc aaaatttaat
     3721 actatcttag tatatcagaa aataaaattt tgtcaatgat ttaaactaaa tatagtggtg
     3781 ttcttttgtt gtttttatat ataaacacaa tatatagtgt ttgtattgtg aaatagcaat
     3841 gattatccta taaattgttt ttgaagaaac tatatcctgt gttttgttat ctaggataga
     3901 agattttcag caagttatct gaaaggaaat tgaaggaaaa tccagaaaat gcttgaaaaa
     3961 aatcctagaa gatggtataa tactaaattg taagggttat cacatataac tcaaaaaaag
     4021 aaagaacaaa aggagagtca aactatggct tctaaagatt tccacgtagt ggcagaaaca
     4081 ggtattcacg cacgtccagc aacattgttg gtacaaactg ctagcaaatt tgcttcagat
     4141 atcactcttg agtacaaagg taaatcagtt aaccttaaat caattatggg tgttatgagt
     4201 cttggtgttg gtcaaggtgc tgacgtaact atctcagctg aaggtgcaga tgcagatgac
     4261 gctatcgctg caatctcaga aacaatggaa aaagaaggat tggcataagg gaaatgacag
     4321 aaatgcttaa aggaatcgca gcatctgacg gtgttgcagt tgcaaaagca tatctactcg
     4381 ttcagccgga tttgtcattt gagactatta cagtcgaaga tacaaacgca gaagaagctc
     4441 gccttgatgc cgctctacag gcatcacaag acgagctttc tgttattcgc gagaaagcag
     4501 taggtacgct cggtgaagaa gcagctcaag tttttgatgc tcacttaatg gttcttgctg
     4561 acccagaaat gatcagccaa atcaaggaaa ctatccgtgc gaagaaagtg aatgcagaag
     4621 caggtctgaa agaagttaca gatatgttta tcactatctt tgaaggcatg gaagacaacc
     4681 catacatgca agaacgcgca gcggatatcc gcgacgtgac aaaacgtgta ttggcaaacc
     4741 ttcttggtaa aaaattgcca aacccagctt ctatcaatga agaagtgatt gtgattgcgc
     4801 atgacttgac tccttcagat acagctcaat tggacaaaaa ctttgtaaaa gcttttgtaa
     4861 ccaacattgg tggacgtaca agccactcag ctatcatggc acgtacactt gaaattgctg
     4921 ctgtattagg tacaaacaac atcactgaaa tcgttaaaga cggtgacatc cttgctgtta
     4981 acgggatcac tggagaagtg attatcaacc cgacagatga acaagcggca gaatttaaag
     5041 cagctggtga agcctatgcg aaacaaaaag ctgaatgggc acttttgaaa gatgctcaaa
     5101 cagtgactgc tgacggtaaa cacttcgagt tggctgccaa tattggtact ccaaaagacg
     5161 ttgaaggtgt taacaacaac ggtgcagaag ctgttggact ttaccgtaca gagttcttgt
     5221 acatggattc tcaagacttc ccaactgaag atgagcagta tgaagcatac aaggctgttc
     5281 ttgaaggaat gaacggtaaa cctgttgtcg ttcgtacaat ggatatcggt ggagataagg
     5341 aacttcctta cttcgatatg cctcacgaaa tgaacccatt ccttggattc cgtgctcttc
     5401 gtatctctat ctctgagact ggagatgcta tgttccgcac acaaatccgt gctcttcttc
     5461 gtgcgtctgt tcacggtcaa ttgcgtatca tgttcccaat ggttgcgctc ttgaaagaat
     5521 tccgtgcagc gaaagcagtc tttgatgaag aaaaagcaaa ccttcttgct gaaggtgttg
     5581 cagttgcgga taacatccaa gttggtatca tgatcgagat tcctgcagcg gctatgcttg
     5641 cagaccaatt tgctaaagaa gttgacttct tctcaattgg tacaaacgac ttgatccaat
     5701 atacaatggc agcagaccgt atgaacgaac aagtttcata cctttaccaa ccatacaacc
     5761 catcaatcct acgcttgatt aacaatgtga tcaaagcagc tcacgctgaa ggtaaatggg
     5821 ctggtatgtg tggtgagatg gctggtgacc aacaagctgt tccacttctt gtcggaatgg
     5881 gcttggatga gttctctatg tcagcaacat ctgtacttcg tacacgcagc ttgatgaaga
     5941 aactcgacac agctaagatg gaagagtacg caaaccgtgc ccttacagaa tgctcaacaa
     6001 tggaagaagt tcttgaactt caaaaagaat acgttaattt tgattaatcg aaaagtccct
     6061 gcaactcagt tacagggact ttttttgata ttttaaaaag aattttcaag aaaatctttc
     6121 ttataaaaag tccaaccttg aaaaagtagt ggtcagaaca aaaaatactt aaatggttca
     6181 taaaattctt gacaagttgg agatttagga gtaaactatt aaccagttaa gtaatagaga
     6241 ggagtttctg caatttagaa atgaattgca actagaaata tcaaatagaa agagagtttc
     6301 gatgaaaatt aataagaaat accttgttgg ttctgcggca gctttgattt taagtgtttg
     6361 ttcttacgag ttgggactgt atcaagctag aacggttaag gaaaataatc gtgtttccta
     6421 tatagatgga aaacaagcga cgcaaaaaac ggagaatttg actcctgatg aggttagcaa
     6481 gcgtgaagga atcaatgctg agcaaatcgt catcaagata acagaccaag gctatgtcac
     6541 ttcacatggc gaccactatc attattacaa tggtaaggtt ccttatgatg cgattttcag
     6601 tgaagaatta ctcatgaaag atccaaacta taagctaaaa gatgaggata ttgttaatga
     6661 ggtcaagggt ggatatgtta tcaaggtaga tggaaaatac tatgtttacc ttaaggatgc
     6721 tgcccacgcg gataacgtcc gtacaaaaga ggaaatcaat cgacaaaaac aagaacatag
     6781 tcaacatcgt gaaggtggga ctccaagaaa cgatggtgct gttgccttgg cacgttcgca
     6841 aggacgctat actacagatg atggttatat ctttaatgct tctgatatca tagaggatac
     6901 tggtgatgct tatatcgttc ctcatggaga tcattaccat tacattccta agaatgagtt
     6961 atcagctagc gagttagctg ctgcagaagc cttcctatct ggtcggggaa atctgtcaaa
     7021 ttcaagaacc tatcgccgac aaaatagcga taacacttca agaacaaact ggctaccttc
     7081 tgtaagcaat ccaggaacta caaatactaa cacaagcaac aacagcaaca ctaacagtca
     7141 agcaagtcaa agtaatgaca ttgatagtct cttgaaacag ctctacaaac tgcctttgag
     7201 tcaacgccat gtagaatctg atggcctt
//
LOCUS       DAVMMR010000076         7147 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMMR010000076 DAVMMR010000000
VERSION     DAVMMR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.076"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(285..1259)
                     /gene="pta"
                     /locus_tag="VYS74_001823"
     CDS             complement(285..1259)
                     /gene="pta"
                     /locus_tag="VYS74_001823"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV3789818.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1303..2199)
                     /locus_tag="VYS74_001824"
     CDS             complement(1303..2199)
                     /locus_tag="VYS74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3789819.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2196..3014)
                     /locus_tag="VYS74_001825"
     CDS             complement(2196..3014)
                     /locus_tag="VYS74_001825"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV3789820.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(2998..3669)
                     /locus_tag="VYS74_001826"
     CDS             complement(2998..3669)
                     /locus_tag="VYS74_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV3789821.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3797..4366
                     /locus_tag="VYS74_001827"
     CDS             3797..4366
                     /locus_tag="VYS74_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV3789822.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4479..5438
                     /locus_tag="VYS74_001828"
     CDS             4479..5438
                     /locus_tag="VYS74_001828"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3789823.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5448..6563
                     /locus_tag="VYS74_001829"
     CDS             5448..6563
                     /locus_tag="VYS74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3789824.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            6568..6915
                     /locus_tag="VYS74_001830"
     CDS             6568..6915
                     /locus_tag="VYS74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV3789825.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            6923..7138
                     /locus_tag="VYS74_001831"
     CDS             6923..7138
                     /locus_tag="VYS74_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3789826.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
BASE COUNT         2203 a         1533 c         1338 g         2073 t
ORIGIN      
        1 aaatttgtcc tttctcgagc ttagctgact tcaacccact acagttgaca aagagccaaa
       61 aaattctctt ttcatactca tttaaaatca aaaagggaat ttatcgtttt acggatatgt
      121 atattacaga tttctatagg tcgtgtatag acaggatatc atcgcttgga agttttcctt
      181 tttgacacca atcagtaatt tagttctcag gaattaatgg cagtgctttt cgtacatagt
      241 ttcataacta cagtatagca tagtatatca ctttatagtt ttcactattg atgaactgct
      301 tgagctgctg tgatgagggt caacttgtaa acatcatctg cattacatcc acgagaaaga
      361 tcattaactg gcttgtttaa accttgcaaa acaggtccta cagccgcaaa gccacccagg
      421 cgttcagcca tcttgtaacc gatatttcct gcctcgatac ctgggaagat gaagacattt
      481 gcttgaccag ctactgtact tcccggagct ttcagagctg cagtttcggg aacaaaggcc
      541 gcatcaaatt gcaactcacc atcgatttca aggtcaggac gcaagtcgtg agcaatttta
      601 gttgcttcaa cgaccttatc aacgctttca ccaaaccctg aacctttagt agaatagctc
      661 aacatggcaa ttttaggttc gatgccaaac atcttagctg tgattgctga gttgatggca
      721 atttcagcca aggcttctgc atctggattg atattaatgg cacagtctcc aaataggtaa
      781 cgttccgtac cacgaaccat gaggaaggct cctgaagtac gagttacatt tggacgagtt
      841 ttgatgattt gtagagctgg gcgaactgtt gaagctgttg agtgaatcgc tcctgacacc
      901 attccatcaa ccaagcccaa gtaaaccaac atcacaccaa agtagttgac atcttcaacc
      961 aaaaccttgc gtacatcttc ttcagtcatt ttgcccttgc gacgctccac caaggcagaa
     1021 accatttctt caaattgagg ataatgttga gggtcgatga cctcataacc atccatgatt
     1081 ccttcaattt caagataaat tttaattttt tcaggatttc caagcaaaac aggaatcact
     1141 tctgtttctt ttactaagcg ttttgttgct tgaagaatac gaggctcttc cccttcaggg
     1201 agaacgatac gagcattttt accaacaagg ttggctttga gactttcaaa aacttccatg
     1261 agttttctcc tttaagataa taattatact ctgagacagt ttttatagag tattagttga
     1321 taactgggta ataaggttac tgaaatcatc cggcaaggga ctttctaact gcaagtcttg
     1381 ctctaaaaat ggatgataaa aggataggta atggcaatgc agagcctgac gttgaatacc
     1441 atcttccaga ctaccaccat acaaatcatc tcccagcaaa ggaaaaccga tatgagaaaa
     1501 atggactcgg atttgatggg ttcgaccagt gtgcaggtga atatagacca agtgaatatt
     1561 tccataagaa gctacaatct tgtatgaagt atgggcatac tttccgcctt tagccactcg
     1621 tctggtaata atggaatctt catcacgcgc aatcggagca ataatttccc cttctggttc
     1681 caaatgtcca tctcccttaa ccaaagcaaa gtagcgtttc tcgatagatt tcttctgcaa
     1741 ctgcttgtct aatcgtgcat gggcataacc gtgcttggca aagagcatca agccagaagt
     1801 gtccctatct agtctggtaa caatgtgaac ctgctgattt tcataatttt gcttgacata
     1861 gtaacccttg ataaaattgg caatggtatt agagtgatta acactaggaa tagaagccac
     1921 tccatagggt ttattcaaga ctagaaagtg gtcatcctca tagagaatat ctaatggaag
     1981 ctcaatagcc tccaaggttt caaagccttt ctcagcggga atgtcaatgg taacgtagtc
     2041 tccaacgtcc aatagatacg ttgcattttg cggttgatta ttgaccagaa tagctccacc
     2101 tcgaaactta atcttggcca gcaatccctt agaaacctcg tgctttttta agaaggtctt
     2161 aaccttgaca tgttcatctg cgataaattc aaacctcatt catccacctc accgataaag
     2221 gcatccttaa cacggttcca gaaactggta tggctaggag tcgcgacaaa gtgaatctta
     2281 tgatgggcga tttgatactc aatacgctca atattacgga aagaataaac gctattgtca
     2341 accgaaatag tatgataatc gtttcttgtt ggaataagtt caatcttatc cttcttaggc
     2401 acaataatgg aagagcccag tgttcgatag acacgattat taaggctggc aatttccgtt
     2461 aattgcaaag cttcaatggt agggtgtaaa acagcaccgc caagagactt gttataggca
     2521 gtactaccag tcggtgtcga aactgttagc ccgtctccac gaaaacgttc aaagggaaca
     2581 ccatttatta caatatctgc caccatggtt cgatcagacc tgcggatgct ggcttcgttg
     2641 agtgctctga aaatcttaac ttcaccattt tcaagaaaga ccttcacatt cagaacaggg
     2701 taagaaaccc ttgccccagt atctagctgc aaattagtca ctagcttgtc caactcaaaa
     2761 tcacgataat ctgtatagaa gcccaaatgt ccagtgtgaa gaccgataaa gcggaccttg
     2821 tcaagctgat tttcgtactt atgaaaggcc gacaagagca taccatcccc gccaatggaa
     2881 atgacaatat ccggattggt atcattgagt ataaactgat ttctcttcaa acgatctcgc
     2941 aattcataca aaaccctttg actctgcggt tttctattgg ctatcagatc aattcgttta
     3001 cctgtattct tcatctgtat cgtcactgtt tcctacaccg tcatttaatt ttctactcaa
     3061 aggatcaaaa agtgcctgag cttcttggat atcatcacga atttcaccca tttcttcatc
     3121 caactgatgg gcgattctag ctgtaatttc cagtcgcttc ttaatctcat ctgggaaatc
     3181 cccttggtac ttgtagttga gagaatgttc tatcgttgcc cagaaattca tggccaaagt
     3241 acgaatttga atttctgcca aaatagtctt aactccattg atggtatcaa ccgtatattc
     3301 tactaccaca tgataggaac gatagcctga tgcttttcta tgagtaatgt aatctcgctc
     3361 ctgtatgatt cgcatatcct gacgcttgtg caaaatatcc actacttcct tgacgtcatc
     3421 tacaaactga accatcacac gtaagccagc aatatcctgc aaatcgtgtt ccaaggtcgc
     3481 ataagtaatg ccacgacgag ccattttttc tttgatgctc tcaattggct taactcgacc
     3541 ggtcacaaac tcaattggag aatgcttatt ttgcttacga tattgcttac gaataccacg
     3601 aagtttaatc tttaactcac caacagcttg aatgtaagga tctagaaatt cttcccattc
     3661 taaggtcata tattctccct tgttctcata ttatccgtca aaaatatctt tgattttttc
     3721 tccagattca tatacaataa atacaatgct tattatacca taaatccgct ttcaacgata
     3781 aaaaaaggta agaaaaatga aacatttaga aattgaattg aaaacactat tgaaaaaaga
     3841 tgaatacaat cgtctaaaag accagttcac aggtgtcact cctgttcttc aaacaaatta
     3901 ctacatcgac acgcctgatt ttgaactgcg agaaaagaaa gtcgctatgc gcattcgaac
     3961 ctttgaagac tgggctgagt tgacactcaa agtcccgcag agtgttggaa acatggaata
     4021 caaccaaaaa ttgcaactaa aagatgctga agattatctg agcaaggaag aacttcctca
     4081 aggactagta ctggatgaat tggcgaaaca tggtatccaa agtaagaact ggcaggttct
     4141 tggttgtcta acaacgcttc gctatgaaat gaaaacagct attggtctca tggcactgga
     4201 tcagagccga tactttgata tgacagatta cgaattggag cttgaagtgg aaaatcatga
     4261 gcaaggcaaa caggatttcc gacaattttt agagaaaaat cagatttcct accaaaaagc
     4321 tccttcaaaa ttggttcgat ttgtcaaaag tatgaaaaat agctgaaata atctccattt
     4381 tttggtaaaa tagaaaagat aaaaatacac aaatcccagt tcatatatgt aaggtaaata
     4441 taactaggat ttataaagtt tacagaggac ggtctataat gtcagataga aaaaacatga
     4501 aacttttcgc actcaactct aaccaagaga ttgcacaaaa aattgcccaa gctgttggtg
     4561 tcccacttgg aaaactatca tcacgtcaat tttcagacgg agaaatccaa gtaaatatcg
     4621 aagaaagtgt ccgtggttat gatgtttaca tcatccaatc aacaagtttc cctgtcaaca
     4681 accacctaat ggaattgtta atcatggtcg atgcttgtgt gcgtgcaagt gcccacagta
     4741 tcaacgttgt ccttccatat tttggctatg cacgtcaaga ccgcattgct tgtcctcgtg
     4801 agccacttac agcaaaacta gttgccaata tgctggttaa ggctggagtt gatcgtatcc
     4861 tgactcttga tttgcatgcc gttcaggttc aaggtttctt tgatattcca gtggataatc
     4921 ttttcactgt tcccctattc gcaaaacatt actgcgataa aggattgctt ggttcagatg
     4981 ttgttgtcgt tagccctaaa aattcaggtg tcaaacgtgc gcgtagcctg gctgaatatc
     5041 ttgatgctcc tatcgccatt atcgactacc ctcaagacga tgcaactcgt aacgaaggtt
     5101 atattattgg tgatgttgaa ggtaagaaag ctatcttgat tgatgatatt ttaaatacag
     5161 gacgtacctt ctctgaagct tctaaaatcg ttgaacgtga aggagctaca gaaatttatg
     5221 ctgtttctag ccacggtctc ttcgtcgagg gagctgctga gcttcttgac aatactaata
     5281 ttaaagaaat tcttgtgact gattcagtag caacaaaaga aaaaactcct aaaaatgtat
     5341 gctacatcac tgctagtgag ttaattggtg atgctatcgt ccgtattcac gaaagaaaac
     5401 cagtcagccc actctttgcc tacaataaaa agaaataagg tgattctttg atttatttgg
     5461 acaatgctgc aacgactcct atgtcagcag ttgctatttc agctatgacc aaggttatgc
     5521 aagaaaccca cggaaatcct tctagtattc atggtcatgg tcgtcaagct ggcaaactct
     5581 tgcgagaagc ccgtcaggaa ctagcccagt tactaaggac aaaacctcaa catatctttt
     5641 tcacttctgg tgggactgaa ggcaataata ctaccatcat tggctactgt cttcgtcacc
     5701 aagaacaagg aaaacatatc atcacaactg ccatcgagca ccatgctgtc cttgaaacaa
     5761 ttgattactt ggttcaacac tttgggtttg aagcaaccat tatccagcca gaaaatcaag
     5821 aaatcacagc ccagcaaatt caaaaggctt tacgtgacga tacgattttg gtttctacta
     5881 tgtttgccaa taatgagaca ggaaacctac ttcccatcgc tgaaattggc caaatactca
     5941 agcaacaccc tgctgcctat catgttgatg cagttcaggc tattggtaaa atcccaattc
     6001 attcagaaga attgggcatt gattttctca ctgcttctgc ccacaaattc catggtccta
     6061 agggaatcgg ttttctctac gcatctagca tggactttga ttcctatcta catggcggag
     6121 atcaggaaca gaaaaaacgt gcaggaactg aaaatctgcc tgccatcgta ggcatggttg
     6181 cagccctaaa agaagaccta gaaaaacaag aagaacattt tcaacatgta caaaatctag
     6241 aaactgcctt tctggcagag ctggagggca ttcagtatta cttgaataga ggaaaacatc
     6301 atctccctta tgttctcaat attggatttc ctggtcagaa aaatgacctc ttactccttc
     6361 ggctagattt agctggaatt tcaatctcta ctggctcagc ctgtactgca ggcgttgtcc
     6421 aatccagcca tgttcttaaa gccatgtatg gcgcaaattc agaacgcttg aaggaatccc
     6481 ttcgcatcag tttgtcgcca caaaataccg ttgaagacct acaaaccctc gcaaaaacct
     6541 taaaagaaat tatcggaggt tagccatatg gcatttgaaa aaatcattca gttaaaaaat
     6601 tgtcgttacg attacactct tagcccttct gttaaaaaat tcaccctcaa agataacacc
     6661 ttttttgaaa ctaaggttgg taactatgaa ctgactcgcc ttttggaaaa agtgccaaac
     6721 agcggtgaag gcttccaact caaaatcatc attaacaagg aacttacagg ggctaaaatc
     6781 aatatcactg acaagtttgg ccttcgtcta gttgatattt tcaaatcaga agaccaccat
     6841 attcatcagg aaaaattcta cttcctcatg gatagcttgg tagaacgtgg tgtctttaca
     6901 aaatcggaaa gatagagcca aaatgtttga actgacctat aaggactgct atcatgtaga
     6961 gcgtactctc aagtatgagg atcatgaggc tctcatgttg actttgtcag gatgtgtaac
     7021 cttaccagat acactttatg tgacttctct gacttttagg ggcaaaaaag tttatcaggg
     7081 gctggtcgga gacctctacc gttttctatc tcatgcagat tttttacatc aaaactaaga
     7141 tttttct
//
LOCUS       DAVMMR010000077         7081 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMMR010000077 DAVMMR010000000
VERSION     DAVMMR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.077"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(80..2077)
                     /gene="metG"
                     /locus_tag="VYS74_001832"
     CDS             complement(80..2077)
                     /gene="metG"
                     /locus_tag="VYS74_001832"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV3789827.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFIMPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2201..3460)
                     /locus_tag="VYS74_001833"
     CDS             complement(2201..3460)
                     /locus_tag="VYS74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789828.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3462..4163)
                     /locus_tag="VYS74_001834"
     CDS             complement(3462..4163)
                     /locus_tag="VYS74_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789829.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4147..5346)
                     /locus_tag="VYS74_001835"
     CDS             complement(4147..5346)
                     /locus_tag="VYS74_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV3789830.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5611..6957
                     /gene="gor"
                     /locus_tag="VYS74_001836"
     CDS             5611..6957
                     /gene="gor"
                     /locus_tag="VYS74_001836"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV3789831.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         2063 a         1562 c         1338 g         2118 t
ORIGIN      
        1 tagcttccta gtttgctctt tgattttcat tgagtataaa taaaaaaaca gaactagcct
       61 gaactagtcc tgtctacttt tacccaatca cacttccatt tggtacagct ggatcaactg
      121 tgagaagggt taatttgcca tcatgttcag ctgagagaat cataccctgg ctgacatatt
      181 ttttcatcat tttacgtggt ttgaggttag caatgatttg aactttcttg ccgaccaatt
      241 cttgttcatt tggatagtat tttgcaattc ctgaaagaat ctgacgatct tctccatcac
      301 cagcatccaa gcggaattga agcaacttat ctgaaccttc tactttagac acttctttga
      361 cttctgcgac acggatttca accttgtcaa agtcttcaaa cttgatttca tccttgttta
      421 gtttgagctc aacttcgtcc ggattccatt ctttttcgac tgctggttta ttgccttcca
      481 tttgttcctt gatataggcg atttcttctt ccatatttag acgtggaaag ataggtgttc
      541 ctttggcaac tacagtcaca tctgctggga agtcagccaa actcaagttt tcaagactag
      601 aaacttcttc caaaccaagt tgagtcaaaa ctgcacgact agtttccatc ataaatggtt
      661 caatcaagtg agcaactaca cgaatgctgg ctgccaagtg gctcatgaca cttgccaatt
      721 ggtcacgaag agcttcatcc ttggccaaga cccatggtgc agtctcatcg atgtatttat
      781 tggtacgaga gatcagagtc cagactgctt caagcgcacg tggatagtca actgcttcca
      841 tgtgtgtatg gaagtctgcg attgattgtt ctgcaacctc agcaagaaca tgatcaaatt
      901 cagtcacacc ttctacatag gcagggattt gtccatcaaa gtacttatta atcatggaaa
      961 ccgtacggtt aaggaggttc ccaaggtcat tagccaattc atagttgata cggccgacat
     1021 agtcttcagg agtaaaggtt ccgtctgaac caactggaag gttacgcatg aggtagtaac
     1081 gaagtggatc tagtccataa cgctctacca acatttcagg gtaaacgaca ttcccttttg
     1141 acttagacat ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa cgatcaggta
     1201 atttaacatc caacatcata agaaggattg gccagtagat agagtggaag cgaaggatat
     1261 cttttcctac catatggaag actgttccat tccagaactt gtcaaagtta ccatgttcgt
     1321 cttgagcgta gccaagagct gtcgcatagt taagaagggc atcaatccaa acgtagacaa
     1381 cgtgttttgg atttgatggg acaggcactc cccatgtaaa ggttgtacga gataccgcca
     1441 aatcttccaa acctggctcg atgaagttgc gtagcatttc attaaggcga ccatctggca
     1501 tgataaattc aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat ttgctaaggc
     1561 gaaggaagta tgattcttca gaaacccatt caacctcatg acctgatgga gcaataccac
     1621 cagtcacatt tccagcttca tcacggaaaa cttctgccag ctggctttct gtaaagaatt
     1681 cttcgtctga tactgaatac caaccagagt attcacccaa gtagatatca tcttgagcaa
     1741 gtaagcgttc aaagacttgt gcgacaactt tttcatggta gtcatcagtt gtacggataa
     1801 atttatcgta tgagatatct agtaattgcc agagttcttt aactccaacc gccattccat
     1861 caacataggc ttgaggtgta ataccagctt cttccgcttt ctgctggatt ttctgaccat
     1921 gttcatcaag acctgtcaga taaaatacat cgtagcccat caggcgtttg taacgtgcta
     1981 ggacatcaca tgcgatagtt gtgtaggcag aaccgatatg aagtttccca gatggatagt
     2041 aaatcggcgt tgtaatataa aaattttttt cagacataat ttttcctttc caggcaaatg
     2101 aaacctgttt ttctaacact tcattatatc acatttttaa tgaatttcga tagggaaatc
     2161 catacaaaaa caagatagac gagtgtccat cttgttgatc tcattcataa cgaagggctt
     2221 caattggatc aagtttcgat gccttgttgg ctggcaagac tccaaaaatc ataccaacac
     2281 tagccgaaac tgcaagacta aatagggcaa ctgggattga tactccaact tctatacctt
     2341 ctattaaacc ttgcagtaac aaacctgcta aggcagttaa accacttgca attgtcaagc
     2401 caattaagcc acctaacaag gtcaaaatca tggattcaat caaaaactga attaaaatat
     2461 tggcacgtgt tgcgcccaaa gccttacgaa gaccaatctc acgagtgcgc tctgtcaccg
     2521 aaaccagcat gatgttcatg acaccagttc ctccaacaaa gagagaaatc cctgcgatgg
     2581 aactaataat cgtcgtcata aaactaaacg attgttgaat ttctgcaaat acaacggact
     2641 catctgccac ctggtattct ccctgttgta agcctgcaag ctctgtcatt tttcgtgcca
     2701 gttctggacc cagagttggg gttaaactgg tatcattcac tcgaaagaca atactagcta
     2761 tttcatctat attaaaattc gcagcaaggg agatattggt agtaataggc aagccaccaa
     2821 acccatatat ttttgacctt ttagcctccg gactagtata aaccccaatg acccggtaac
     2881 taaatccatt gacttctaca accttgttaa tagcctcttg aggagattca aataaactaa
     2941 tggacaattc ctcatctagc aaaataacac ttgcaaactc tttgaaatct tgctctctca
     3001 gactacgacc tgcaataatt tcattcttaa cagcgtccat gtaagttctg tttccacctg
     3061 tcaaattagc attctcaacc tttttatctt gataggtcaa gatagcattc gttgaattgg
     3121 ttacatagta actatccact cccttcagtt tagctgcctc ttggacccag gattcttgcg
     3181 gttttggcgg ttcaacagga atttcctctt cctttccaga aaccgtaaaa gatgattgtt
     3241 tctgagtaaa agaaccatct ttactttttt taggagagaa aaagacgcta atatttttct
     3301 gagatttagt catatcttta ttgacttgac gagataggga atcacccaaa gccataatca
     3361 caacaactga tgaaacaccg ataataatcc caatcatagt aagcaaagaa cgcatcttgt
     3421 gagccatgat agatgaaaag gcaaatttca gattctgcat cttagttttc ctcctttcct
     3481 aactgagcac tgtcagacga aatgacccca tcccgaatga caatctgacg tttagcatag
     3541 gcagcaatct caggctcatg cgttaccatg ataatggttt ttccttcttt attcaaatca
     3601 accaataatt gcataatttg gttacctgtt ttggtatcca aggctcctgt cggttcatcc
     3661 gctaggataa tagaaggatt gtttaccaag gcacgcgcaa tggctacacg ttgcttttga
     3721 ccaccagata attctgaagg taaatggtga ctacgttctg tcaattcaac cttgtctaaa
     3781 tattcctcag ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat caagggcaat
     3841 tctacatttt gcagagcatt gagcttcgat agaagaaaga actgctgaaa gacaaaaccg
     3901 atttgttggt tacggacctt agccagttgt ttttcaccaa gcccagccac ttcttgacct
     3961 tcaagataat attctccact ggttggtgta tccaacatgc caatcgtatt catcagagtg
     4021 gacttaccag acccagatgg tcccatgatg gctacaaatt caccctcatt cacttctaga
     4081 ttgatatttt tgagaacctg cagttcttgg tcaccattac ggtagcttct gaagatattt
     4141 tttagactaa ttagttgctt catcagcctt cacctctttt ccttcttcca aggaagatgt
     4201 tggattactg atgaccttag caccattcgt taaaccagaa gtgatttctt gattttctgc
     4261 gtcagcattt cccaatgaaa cctcaacttt tttagccttt tgttgttcat ccacaatcca
     4321 gacataattt ttactatcat ccattactag actgctaaca ggaacaagaa tagccttagt
     4381 tttgctttta acctcaatgt tgacagaaaa accctgtttc aaatcaccaa cctcgcctgt
     4441 cacatcaata gtataagggt atttagaacc tgtattattc ccggctgctg gactagctgc
     4501 ttcaccattg tttttaggat agtcagaaat atagcttaat ttcccagtcc attttttatc
     4561 aggatacact ttagaagtaa agcttacttc ttgacctaca gaaaggttgg ctagattgta
     4621 ctcagacaat tctcccttga cttgtaaatt ttcattgctg acaatatgaa ccataacttg
     4681 gctcgcccct gttggagatt tagaaacatt gctattgact tcgactacag ttccctctag
     4741 ggtactgaga acagttgttg catccaattg actttgagcc ttgcttaatt gcgctgcagc
     4801 atctgcacgc gcatcacggg catcacccaa ttgagcgtca atagaagcaa cagaatttcc
     4861 agccactgga gttgggcttt gcaccgttgc atcttctcct cctactggcg ctggtaactg
     4921 tggagccgga gctgaagcgg cttcatttcg tgcttgattg agttcattga tatgacgatc
     4981 tgccttagct actgctcgac tagctgaatc ataggccgcc tgcgcttctg aactactgta
     5041 cttgactaaa gcctgccctt cgctgacctt atcgcccaca gaaacaagga tttcatctaa
     5101 atcaccctta ctagcatcaa aataaacata ttgttcattt tttgctgtta ctgtccctga
     5161 caataaaaca gaggaggcca cgcttccttc cttggcaaca acaagatgag taggctcatc
     5221 ttttagagca gtctgagaag gttgtctaaa gagtaaaatc cccccagcac ccaatacaac
     5281 tacactcgca gcaccgattg ctgcatacag ttgccacttt ttagctttac cattcttttt
     5341 cttcataatg aaactccttt tcttttttac aatactttgc tattatacca aatttccctc
     5401 cagcaaacaa tacagttcag gattaaacaa tcgttcggaa ttttgctttt ctattgtact
     5461 agatatataa tacactatct tgacttattt ggcaagcttt tttgcaattt tttgtgcgta
     5521 gcagattttt gcaatcaaat caaaaaaaga ataaaatata actatcaaaa aagacgctct
     5581 actatttaaa agattacaaa ggagttttca atgagagaat atgatatcat tgctatcggt
     5641 ggaggtagcg gaggaatcgc taccatgaac cgtgctggtg aacatggagc caaagcagcc
     5701 gttattgagg aaaagaaatt aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa
     5761 atcatgtggt acggggcgca aatcgctgag actttccatc aatttggaga agactacggc
     5821 tttaagacta ctgatcttaa ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac
     5881 attgatcgcg ctcgttcttc ttatgatggt agttttaaac gcaacggtgt agacttgatt
     5941 gaaggtcatg ctgaatttgt agattctcat actgtaagcg taaatggtga actgattcgt
     6001 gctaaacata tcgtgattgc tacaggtgcc catccaagta ttcctaatat tcctggtgct
     6061 gagctaggtg gctcttctga tgatgtattt gcctgggaag aacttccaga gtcaattgcc
     6121 attctaggcg ctggttatat cgccgttgaa ttagctggcg tactccacac ttttggtgtc
     6181 aagacagatc tctttgttcg ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt
     6241 gaaggtttgg tcaaggaaat ggaaagaaca aacttaccac ttcacactca caaagtccct
     6301 gtcaagttag aaaaaactac tgacggcatt accattcatt tcgaagatgg tactagtcac
     6361 acagctagcc aagttatctg ggctacaggt cgccgtccaa acgttaaggg cttgcaactt
     6421 gaaaaagctg gagtgactct gaacgaacgt ggctttatcc aagtggatga ataccaaaat
     6481 actgttgttg agggaatcta tgctctaggt gatgtaacgg gcgagaaaga actgactcca
     6541 gttgcaatca aggccggacg taccctatct gaacgtctct ttaacggaaa aactactgca
     6601 aaaatggatt actcaactat tccaactgtt gtcttttcac accctgctat cggaactgtt
     6661 ggtttgacag aagagcaagc tattaaagaa tacggtcaag accaaatcaa ggtttataaa
     6721 tcaagctttg catctatgta ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa
     6781 ctcataacag ctggttcaga agaaaaagtt gtcggacttc atggaattgg ctacggcgtt
     6841 gatgaaatga ttcaaggatt tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt
     6901 gatgcaactg tggcaattca cccaactgca tctgaagaat ttgtaaccat gcgttaatcg
     6961 aaacaaaaga agcaaagcaa aaaacacttc taaatatcaa aaagcgagta tttccgtagt
     7021 tggagatact cgttttctta tgttttattt tatagtatgt tgaagtaaga tatgcaaaaa
     7081 t
//
LOCUS       DAVMMR010000078         6914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMMR010000078 DAVMMR010000000
VERSION     DAVMMR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.078"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(59..358)
                     /locus_tag="VYS74_001837"
     CDS             complement(59..358)
                     /locus_tag="VYS74_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV3789832.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            760..1464
                     /locus_tag="VYS74_001838"
     CDS             760..1464
                     /locus_tag="VYS74_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV3789833.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    1523..1610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1646..1960
                     /gene="rplU"
                     /locus_tag="VYS74_001839"
     CDS             1646..1960
                     /gene="rplU"
                     /locus_tag="VYS74_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV3789834.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1976..2320
                     /locus_tag="VYS74_001840"
     CDS             1976..2320
                     /locus_tag="VYS74_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV3789835.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            2337..2630
                     /gene="rpmA"
                     /locus_tag="VYS74_001841"
     CDS             2337..2630
                     /gene="rpmA"
                     /locus_tag="VYS74_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV3789836.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2941..3146)
                     /locus_tag="VYS74_001842"
                     /pseudo
     CDS             complement(2941..3146)
                     /locus_tag="VYS74_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            3583..4500
                     /locus_tag="VYS74_001843"
     CDS             3583..4500
                     /locus_tag="VYS74_001843"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV3789837.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(4593..5441)
                     /locus_tag="VYS74_001844"
     CDS             complement(4593..5441)
                     /locus_tag="VYS74_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3789838.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            5582..6421
                     /locus_tag="VYS74_001845"
     CDS             5582..6421
                     /locus_tag="VYS74_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3789839.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            6528..6803
                     /locus_tag="VYS74_001846"
     CDS             6528..6803
                     /locus_tag="VYS74_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV3789840.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT         2182 a         1151 c         1405 g         2176 t
ORIGIN      
        1 catttttgca cctttcttag cctttgttat cgacaagcat tgttctagct ccttgttttt
       61 agaaaggtaa tttcccagcg aaagcaccca gttttttctt ggtagtttca tcgatttgtt
      121 caagagcaga gttgatggct tgaacggtca tatcagaaag agtctcaagg tcctctgggt
      181 caacgacagc tggattgaaa tcaatgctga caactttctt atcgccagtt aaggtcgctt
      241 ggacaagatc ttgagcagat ttgccaacaa attgcatagc agcaagttca gcttggcttt
      301 gttccatttg tttttgaagt ttttgtgctt gacgcatcat gttttgcatg ttcatcataa
      361 gatttctaaa gttcctttca tctcaagggg ataatccttg ttgttaacaa gtgcctgatt
      421 ttctctccta attgttatta gtagcatagc catcaagccc aagtattgat tttattatac
      481 cattttttta agacatagtc ttttattggt gagagttatt tatgtcaagg agtgattaga
      541 aataaattaa aataatgatt tttgctaaga gggctctatt ctagtttcat ggaaatgatt
      601 ttactaaagg ttcgtatgct ttgtttgcaa acatgttaca tctatctatt tttggataaa
      661 tagttgtcta ttattatttt tatgctaata gtaaaatcta aatggtagcc tacgattttt
      721 tagaaagaaa atggtacaat atttctaaga gaaaatacaa tgggaggtaa aatgaggtta
      781 ttacctataa gaaaaatatc acgtcagtct aaaaggttag cacttttttt gacgttttgt
      841 gctggatatg tggatgctta cacttttatt gttcgcggga atacccttgt agctggacaa
      901 actggaaatg ttgtctttct ttcagtagaa ttaattaaaa ataatgtttc ggatgttagg
      961 gacaaggttc tcaccttgct agcgtttatg atgggagtct ttttattaac gatttataag
     1021 gaaaaattga gaattgtgaa aaaacctatt ctgtccttga ttcccttggc aatcttatca
     1081 atcattattg cttttgtgcc gcaaactgtg gataatatct atctagtgcc gcccttggcc
     1141 ttctgtatgg gactggtgac aactgctttt ggagaagtgt cgggtattgc ctataataac
     1201 gcttttatga cagggaatat caaacggacc atgctggctt ttggagatta tttccgaacc
     1261 aagcacactc cttttttgcg tgaaggattc atatttgtta gcctgcttag tagttttgtc
     1321 cttggcgttg tcttttcagc ctatttgacg attttctatc atgaaaagac cattcttggt
     1381 gttcctatta tgatgagcgt tttttacctc agcatgcttt ttgcctcttg gcagaaaaaa
     1441 gtaaaagaaa aagcttcatt ttaggtgtta ttacttgtaa gaaaatagga gatatgctat
     1501 acttgaagag ttgactatgc acgatcctgt gcaaccgcac gaacatcgtt gcttgattat
     1561 tcaagtttaa gtggttcgtc ccacgatgct aggcgagtct tcacaaaaaa tcggggaaac
     1621 ccataaaaat cataggaggt gcataatgag cacatacgca attatcaaaa ctggcggaaa
     1681 acaagttaaa gttgaagttg gtcaagcagt ttacgttgaa aaattgaacg ttgaagctgg
     1741 tcaagaagtt acttttaacg aagttgttct tgttggtggt gaaaacactg ttgtcggaac
     1801 tccacttgtt gctggagcta ctgtagttgg aactgttgaa aaacaaggaa aacaaaagaa
     1861 agtggttact tacaagtaca aacctaaaaa aggtagccac cgtaaacaag gtcaccgtca
     1921 accatataca aaagttgtca tcaacgcaat caacgcttaa ttttaaggag aacacatgat
     1981 acaggcagtc tttgagagag ccgaagatgg cgagctgagg agtgcggaaa ttactggaca
     2041 cgccgagagt ggcgaatacg gcttagatgt cgtgtgtgca tcggtttcta cgcttgccat
     2101 taactttatc aattctattg agaaatttgc aggctatgaa ccaatcctag aattaaacga
     2161 agatgaaggt ggctatctga tggttgaaat accaaaagat cttccttcac accagagaga
     2221 aatgacccag ttattctttg aatcattttt cttaggtatg gcaaacttat cggagaacta
     2281 ttctgagttc gtccaaacca gagttatcac agaaaactaa cacggaggaa aacattatgt
     2341 taaaaatgac tcttaacaac ttgcaacttt tcgcccacaa aaaaggtgga ggttctacat
     2401 caaacggacg tgattcacaa gcaaaacgtc ttggagctaa agcagctgac ggacaaactg
     2461 taacaggtgg atcaatcctt taccgtcaac gtggtacaca catctatcca ggtgtaaacg
     2521 ttggtcgtgg tggagacgat actttgttcg ctaaagttga aggcgtagta cgctttgaac
     2581 gtaaaggacg cgataaaaaa caagtttctg tttacccaat cgcaaaataa aaaggttcat
     2641 tgaacctttt atcccgaacc ttgaaatgta aaggtgagga agctagaaac agcttaaaat
     2701 aaggctttcc gagttttcgg agagcctgtt tttttatttt gatacccata ctttttaata
     2761 tgtatagtgg attgatactc ttcgaaaatc aaattcaaac cacgtcaatt tcaccttgcc
     2821 gtactcaagt acagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatg
     2881 aatctggaat agaacactgt taaaaattga tatgagtttc ctactcgatt tcttcagatt
     2941 ttatttcaat tgactataag aactgctatc tggtttcaaa tattggcttg tcgtcccttc
     3001 acttgtctga atggtttgag ccgttccttc tgataaggga aaagatattc tttagctgaa
     3061 acggtctgag aatccaagct aacagatgga gagaaaaata gcccagtctg aagcacacta
     3121 gatgggagtt gttggaaagc agtcatctag tttttctttg ctgttttggc ttttttcttt
     3181 gtcatcaggg aaagtgactt gcttcaaatc agggagttta ggaaattctt caaacatctt
     3241 gcccaagttg tctcattttg taaattttac agccataggt gtacctcgat tctaaattct
     3301 acctttttgg caccaattag taatttaaag ttctcaggag ttagttgcat tgctttttca
     3361 tacataacat agtatgacac tttatggtta ccgaacaagt ctaatcactc cttgcaaaat
     3421 tttgaagatg tcctacaagt ttttgagaag aatattgcaa agttcatagt taagcaacga
     3481 tagaataaag tgctttttga aaacaggtac acttcatgtg aaaaaaacaa catttcagat
     3541 tttctgttcc agaaatagct atcgctatga tataattatt ttatgattat tactattcct
     3601 ataaaaaacc aaaaagatat tggcacacca tctgattcag tcgttgttct cggctatttt
     3661 gatggcatac ataaggggca tcaagaatta tttcgtgttg ccaacaaggc tgcgagaaag
     3721 gatttattgc ctatcgtcgt tatgaccttt aatgaatctc caaagatcgc tttagagcct
     3781 tatcatccag atttgttttt gcatattttg aaccctgctg aacgtgaaag aaaattaaag
     3841 cgcgaaggtg tagaagaatt atatctcctt gattttagta gtcaattcgc tagtctcact
     3901 gcacaagaat tctttgcaac ttatatcaag gctatgaatg ccaaaattat tgttgcaggt
     3961 tttgattata catttggttc tgacaaaaaa acagcagaag atttaaagga ttactttgat
     4021 ggagaagtta tcattgttcc acctgtagaa gatgagaaag gaaagattag ttcaactcgt
     4081 atccgtcaag ctattttaga tggaaatgtg aaagaagcag gaaaactttt gggggcaccg
     4141 cttccatcaa gaggtatggt ggttcatggt aatgctcgtg gtcgtacaat tggttatccg
     4201 acagcgaatt tagtgctttt agaccgtact tatatgccag cagatggcgt ttatgtcgtt
     4261 gatgttgaga ttcaaagaca gaagtatcgt gctatggcta gtgtcgggaa aaatgtgacc
     4321 tttgatggag aagaagcacg ttttgaagtc aatatttttg attttaatca agatatttat
     4381 ggggaaaccg tcatggttta ttggcttgat cgcattcgtg atatgaccaa atttgactca
     4441 gttgaccaat tagtggatca gttaaaggct gatgaagaag taactcggaa ttggtcttaa
     4501 gagcttgagt aaataaaaca aaaaagaggt tgtctgtaac ccaaaagata gatgatttag
     4561 tctaactttt gaggtcacga cattacctct ttttattctt tttcaaaggt gaagccttct
     4621 cctaggattt catgggcttc tgtaatagtt ataaaggctt gaggatcgat tcgatgaatc
     4681 atttccttcg ttttcacaat ttcatttctt ccgacaatac agtagatgat tttcaaattt
     4741 tctttactat agtagccttg accagaaata aaagtaacac ctcttccgag gtcatcatta
     4801 atcgccttag caagttggtc aggacgtttt gtgataatca taaagccttt gccggcatat
     4861 cctccttcac caatcaaatc aataacacga gaaacaataa aatcaaacaa aagcgtgtag
     4921 gaaaccaatc tcaaatcctt gaagattagg agaatcaaca tgagaataca aaaatctaag
     4981 ataaagagca gttttcctat ggatatatga gtgtatttgt tgagaatacg agctagaata
     5041 tcagttccgc cagttgtacc tccagcatta aaaataattc caaggccaat tcccaatagg
     5101 attcccgcta taagggctgt gattagtaaa tcaccttgaa gatcaatatg aaggggaata
     5161 tgctcaaaaa aagctaacca ggcggacaaa gctaaggttc ctagtaaact agaatagagg
     5221 gatttggctc caaagatctt ccaagctagg atgaaaaggg gaatattaat cagcaggttc
     5281 atgagggaaa cagggatttt aaaaagataa aaggtgatga gggtaatgcc tgtcgcccct
     5341 ccttcaaaga gatgatgggg aactacaaaa taagtcagtc caaaagcata aatagcagca
     5401 cctagtaaaa tggtaaaaat ggggtaaatt tttttaatca taggacacct cttttcttat
     5461 aagttgatta tatcaaattt tacggaaaat ttgattgact ccattaggat ttttaatctt
     5521 tttttgatat aattagaagt aaggaaacca atctgaatcc taaaatagaa agattgatac
     5581 tatgacaaaa attaagattg taaccgattc atctgttact attgaacctg aactagtaaa
     5641 gcaattagat attacaattg ttccattatc tgtaatgatt gataatgttg tttattctga
     5701 tgcggatttg aaagaagaag gtaaatttct tcagttgatg caagaaagta agaatcttcc
     5761 gaaaacaagt cagccacctg taggtgtctt tgctgagatt tttgaagacc tatgcaaaga
     5821 tggtggccag attcttgcta ttcatatgtc ccatgctctt tcgggtacgg tagaagcagc
     5881 acgccaaggt gctagcctat ctactgcaga tgtgactgtt gttgatagtt ccttcactga
     5941 ccaagccctg aaattccaag ttgttgaggc tgcgaagtta gctcaagaag gtaaagatat
     6001 ggaggcaatt ttatctcatg tagaagaggt taaaaaccat acagagcttt atattggtgt
     6061 ttcaactttg gaaaatcttg tcaaaggtgg acgaattagc cgtgtaactg gcttgttgag
     6121 ctctcttctc aatatccgtg ttgtcatgca aatgaaagac catgaattgc agccaatggt
     6181 taaaggtcgt ggaactaaaa catttaaaaa atggttagat gagttgataa catcgctttc
     6241 tgaacgtgct gtagcagaga ttggaatttc atattctggt agtgatgatt gggcaaaaga
     6301 gatgaaagaa agcttacaag cttatgttga aaagccaatt tctgttttgg aaacgggttc
     6361 cattattcaa actcacacgg gtgagaacgc ttgggctatt ttgatacgtt accactccta
     6421 aaaaaataaa taaaatggga agaaatagcg tttttaactt gacctaaaag ggattttagg
     6481 atatgattat atttgttaat tagaaattat ttggaggaat cattaacatg gcaaacaaac
     6541 aagatttgat cgctaaagta gcagaagcta cagaattgac taagaaagac tcagcagcag
     6601 cagttgaagc tgtatttgca gcagtagctg actatcttgc agctggtgaa aaagttcagt
     6661 tgatcggttt tggtaacttt gaagttcgtg agcgcgcaga acgtaaaggt cgcaacccac
     6721 aaactggtaa agaaatgaca attgcagctt ctaaagtacc agcattcaaa gctggtaaag
     6781 ctcttaaaga cgctgttaaa taatcagtct ttaaaaagcc tattgtatca agctttctag
     6841 cttggtcagt aggcttttta attatgaaat ctagtaataa atagagttaa aaagttgaat
     6901 gttccgaaaa tatt
//
LOCUS       DAVMMR010000079         6773 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMMR010000079 DAVMMR010000000
VERSION     DAVMMR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.079"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(1..203)
                     /locus_tag="VYS74_001847"
                     /pseudo
     CDS             complement(1..203)
                     /locus_tag="VYS74_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            401..2167
                     /gene="patB"
                     /locus_tag="VYS74_001848"
     CDS             401..2167
                     /gene="patB"
                     /locus_tag="VYS74_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV3789841.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2306..2995
                     /locus_tag="VYS74_001849"
     CDS             2306..2995
                     /locus_tag="VYS74_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3789842.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            2999..3178
                     /locus_tag="VYS74_001850"
     CDS             2999..3178
                     /locus_tag="VYS74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789843.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3165..4514
                     /locus_tag="VYS74_001851"
     CDS             3165..4514
                     /locus_tag="VYS74_001851"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV3789844.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4638..6098
                     /locus_tag="VYS74_001852"
     CDS             4638..6098
                     /locus_tag="VYS74_001852"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV3789845.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            6112..6543
                     /locus_tag="VYS74_001853"
     CDS             6112..6543
                     /locus_tag="VYS74_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3789846.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
     gene            6667..6738
                     /locus_tag="VYS74_001854"
     tRNA            6667..6738
                     /locus_tag="VYS74_001854"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6700..6702,aa:Glu,seq:ttc)
BASE COUNT         2134 a         1291 c         1460 g         1888 t
ORIGIN      
        1 tgataaatag aagaatccag tccagcgaaa gcttgtaatt gagcaggatt atcaaaggca
       61 tgaatatttc gaatctcggc taaaatgacc gcccctaaac gattctcaat cccagtaacc
      121 gtcgtgatga ccgagttgaa ctcagccatc aagtcattga cacatttttt cgccttgtca
      181 atgagcctct tgtaatgttt gatgttttca ttacaagaga taaaacgttt atgcgttatc
      241 aaactcatta ccaattgaaa caaaaagctg tggttagatc ctttcggaaa ttgtcaagcg
      301 attggaggaa atgaactaat ccacagcggc ttattccaag tataccactt gggctttggc
      361 agtagctaac tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt
      421 ttggcattat tttaaggtct acaagttctc atttgtagtt gtcatcctga tgattgttct
      481 ggcgactttt gcccaagccc tctttccagt cttttctgga caagcggtga cgcagctagc
      541 caatttagtt caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg
      601 aatcatggtc aatcttggcc tgctggtttt ggttctattt atctctagtg taatatacat
      661 gtgtctcatg acgcgcgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg
      721 taagcttgct cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcctgtc
      781 tcattttacc agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt
      841 catgagcaat attgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac
      901 gctggctctc atcaccattg ccagcacccc attggctttc cttatgctga ttttcatcgt
      961 gaaaatggca cgtaaataca ccaacctcca gcagaaagag gtagggaagc tcaacgccta
     1021 tatggatgag agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat
     1081 gatggcagga tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat
     1141 gttctcagga attcttttcc ctgtcatgaa tgggatgagc ctgattaata cagccatcgt
     1201 catctttgct ggttcggctg tacttttgaa tgataagtct attgaaacaa gtacagccct
     1261 aggtttgatt gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt
     1321 tgcagcgagt tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat
     1381 gtttgatgca gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga
     1441 aagtgttgaa atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga
     1501 tgtcagcatt tctgccccta aaggccagat gacagcagtt gttgggccta caggttcagg
     1561 aaaaacgact attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta
     1621 ttttgatggt aaagacattc gtggctatga cttagatagt cttagaagca aggtgggaat
     1681 tgtattgcaa gattcggtct tgtttagcgg aacgattcga gacaatatcc gatttggtgt
     1741 gccagatgct aatcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta
     1801 tatcgaaagt ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc
     1861 aacagggcag aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct
     1921 cattctcgat gaagcaactt caaacgtaga tacggtgacg gaaagcaaga ttcagcatgc
     1981 catggaggtg gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat
     2041 tctcaatgca gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca
     2101 ccatgaactt ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt
     2161 cgaataagaa agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat
     2221 cacagcctta aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc
     2281 gttagtattc gaaaggagag gcatcatggc tagaacggtt gtaggagttg ctgcaaatct
     2341 atgtcccgta gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga
     2401 gatcattcgt caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt
     2461 acgtgattat gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc
     2521 tcagttttat ggagagaaaa agaccgtcga aagcgatgat tacaatctgg tccgtgacga
     2581 atttgaattg gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg
     2641 tcgcggtgtc caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg
     2701 tcactggcaa ggactacctt tcgggacatc tcactctatt gagacagtag aaggaagcgt
     2761 ggtggctaag ctatttggga aagaaagtca ggtcaactca gtccatcgtc aaagcattaa
     2821 agatttggca cctaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc
     2881 gattgagtct atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt
     2941 taatgaagaa gatggcaatt tagaattatt tgagtattta ttgaatgaac tgtaacgatt
     3001 ggaacatctg gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gcattttacg
     3061 caaacgtttg aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga
     3121 aggtaagcgt tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat
     3181 ttgattattc aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat
     3241 cacaagtaac agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct
     3301 tgggatggtt ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag
     3361 ctgctgagca aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt
     3421 accttggtgc aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa
     3481 aagaagaacg caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc
     3541 ttgctgactt ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat
     3601 caggtacaac aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga
     3661 aatacggtca agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg
     3721 ctgttaaggt tgaagcagac gctaacggtt ggggaacatt tgttgttcca gatgatatcg
     3781 gtggacgctt ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg
     3841 acatcaaagc tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa
     3901 tctctgaaaa cgaagcttac caatacgcag tcgttcgtaa catcctttat cgtaaaggct
     3961 atgcaactga gatcttggta aactatgagc catcacttca atacttctca gaatggtgga
     4021 aacaattggc tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca
     4081 acttctcaac tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt
     4141 ttgaaacagt tgtccgtgtt gacaaacctc gtaaaaacgt gcttattcct actttggaag
     4201 aagaccttga cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag
     4261 caactgacgg tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc
     4321 ttccagagca agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg
     4381 ccctttcagg ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata
     4441 aacgtaacat gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta
     4501 acgcacgtct ataatagaag aaaagagtgg tttgcccact ctttttactc tctttatcca
     4561 tagaaattgg actcagccaa gacttgtgat ataatataga aagcaaaaag gcagacgcct
     4621 agataatagg agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag
     4681 gactactaca catcggaaat gcccgtacag cattgtttaa ctacttgtat gcgcgccatc
     4741 atggtggaac atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg
     4801 gtgaacgttc acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag
     4861 aatcacatga gaattatcgc cagtctgagc gtttggactt gtatcaaaaa tacattgacc
     4921 aactattagc tgaaggaaaa gcctataaat cttacgttac agaagaagag ttggcagctg
     4981 aacgtgaacg tcaagaagta gctggcgaaa caccacgcta catcaatgaa taccttggta
     5041 tgagtgaaga agaaaaagcg gcttatatcg cagaacgtga agcagctggt atcataccaa
     5101 cagttcgttt ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggtg
     5161 atatcgaatt tgaaggtggc aatatcggcg gtgactgggt tatccaaaag aaagacggtt
     5221 acccaactta caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta
     5281 tccgtggaga tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg
     5341 gttgggaagc tccagagttt ggtcacatga ccttgattat caactctgag actggtaaga
     5401 agttatctaa acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt
     5461 accttccaga agcagtcttt aactttattg ctcttcttgg ttggaaccca ggtggtgaag
     5521 atgagatttt ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt
     5581 caccagcagc ctttgatcag aaaaaattag actggatgag caatgattat atcaagaatg
     5641 cagaccttga aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga
     5701 ctgacaaggc tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga
     5761 ttatcccatt gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg
     5821 aagtcatgac gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag
     5881 cgatgacaga tgatgaattt gtgacagaaa atatcttccc acaaattaaa gcagtccaaa
     5941 aagaaacagg tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg
     6001 aaatgcatgg gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc
     6061 agcatatcga aaacattcta aaagaaatct ctaaataaag aggatacaat aatggacatc
     6121 tgggaaaaga tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt
     6181 gtttatgcca atcatgttgt agctgcagta gaagcaggag atggacaaat atttacagga
     6241 ttctgtatgg agggaacctg tggtgttttc catctctgcg cagaacgggc gaccctcttc
     6301 aatatgtacc aattttcagg acaaactaag gttaagaaag tattagcctt tcgagacaaa
     6361 ccaccttatg gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagag
     6421 cttaatgctg aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt
     6481 aaagtcgcag aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag
     6541 tgatagaaga gtcagtataa ttctggctct ttttactata agttgaaaaa ataaaaaaaa
     6601 ttagaaaaag tagttgacaa aatttgaaaa gactgtataa tagtaagagt tgaaaataac
     6661 aactcaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt
     6721 cgaatcccgt acggactatg gtatgttgcg tcaggagcac ttgatgaaaa aaa
//
LOCUS       DAVMMR010000080         6564 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMMR010000080 DAVMMR010000000
VERSION     DAVMMR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.080"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            165..365
                     /locus_tag="VYS74_001855"
     CDS             165..365
                     /locus_tag="VYS74_001855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV3789847.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            400..1116
                     /gene="atpB"
                     /locus_tag="VYS74_001856"
     CDS             400..1116
                     /gene="atpB"
                     /locus_tag="VYS74_001856"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV3789848.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1130..1624
                     /gene="atpF"
                     /locus_tag="VYS74_001857"
     CDS             1130..1624
                     /gene="atpF"
                     /locus_tag="VYS74_001857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV3789849.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1624..2160
                     /locus_tag="VYS74_001858"
     CDS             1624..2160
                     /locus_tag="VYS74_001858"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV3789850.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2175..3680
                     /locus_tag="VYS74_001859"
     CDS             2175..3680
                     /locus_tag="VYS74_001859"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV3789851.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3696..4574
                     /locus_tag="VYS74_001860"
     CDS             3696..4574
                     /locus_tag="VYS74_001860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV3789852.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4660..6066
                     /gene="atpD"
                     /locus_tag="VYS74_001861"
     CDS             4660..6066
                     /gene="atpD"
                     /locus_tag="VYS74_001861"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV3789853.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6077..6496
                     /locus_tag="VYS74_001862"
     CDS             6077..6496
                     /locus_tag="VYS74_001862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV3789854.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT         1952 a         1228 c         1451 g         1933 t
ORIGIN      
        1 tacaagtcac ccagagaaga cttctggatg gctaacttga acttgaaatt tagcaaaatg
       61 gaaataattt ttattaaacg cttccaatag cggttaaaag ttgacaaatt taaattttag
      121 gactacacta agtaagtaaa ttttatttta aaggagaatt tgttatgaat ttaacatttt
      181 taggcttatg tattgcctgt atgggcgtat ctgtcggtga aggtttattg atgaatggac
      241 tgtttaaatc agtagcacgc caaccagata tgctttctga gtttcgtagt ttgatgtttt
      301 taggtgttgc ctttattgaa ggaactttct ttgtaactct tgtcttctca tttattatca
      361 aataaataca tggaacgaga agaaaaggga ggattttaga tggaagaaag tattaatcca
      421 atcatctcta ttggtcctgt tatcttcaat ctgactatgt tagccatgac tttgttgatt
      481 gtgggagtta tttttgtctt tatttattgg gcaagccgca atatgacctt gaaacccaaa
      541 ggaaagcaaa atgtacttga gtatgtctat gactttgtta ttggatttac agaacctaac
      601 attggttcgc gctacatgaa agattactca ctctttttcc tttgtttatt ccttttcatg
      661 gtgattgcca ataaccttgg cttaatgaca aagcttcaaa cgatcgatgg gactaactgg
      721 tggagttcgc caaccgctaa tttacagtat gacttaacct tatcttttct tgtcattttg
      781 ttgacacata tagaaagcgt tcgtcgtcgt ggatttaaaa aaagtataaa atcttttatg
      841 agtcctgttt ttgtcatacc gatgaatatc ttggaagaat ttacaaactt cttatctttg
      901 gctttgcgga tttttgggaa tatctttgca ggagaggtca tgacgagttt gttacttctt
      961 ctttcccacc aagctattta ttggtatcca gtagcctttg gagctaattt ggcttggact
     1021 gcattttctg tctttatttc ctgcatccaa gcttatgttt ttactctttt gacatctgtg
     1081 tatttaggga ataagattaa tattgaagag gaatagaaag gagtaactga tgcacgtaac
     1141 agtaggtgaa ttaattggta attttatttt aatcactggc tcttttattc ttttgctagt
     1201 cttgattaaa aaatttgcat ggtctaatat tacaggcatt ttcgaagaaa gagctgaaaa
     1261 aattgcttca gatattgaca gagctgaaga agcccgtcaa aaagcagaag tattggctca
     1321 aaaacgcgaa gatgaattgg ctggtagccg taaagaagct aagacaatca ttgaaaatgc
     1381 aaaggaaaca gctgagcaaa gtaaggctaa tatcttagca gatgctaaac tagaagcagg
     1441 acacttaaaa gaaaaagcca atcaagaaat tgctcaaaat aaagtagaag ctttacagag
     1501 tgttaagggt gaggtcgcag atttgaccat cagcttagct ggtaaaatca tctcacaaaa
     1561 ccttgacagt catgcccata aagcactcat tgatcagtat atcgatcagc taggagaagc
     1621 ttaatggaca agaaaacagt aaaggtaatt gaaaaataca gcatgccttt tgtccaattg
     1681 gtacttgaaa aaggagaaga agaccgtatc ttttcagact tgactcaaat caagcaagtt
     1741 gttgaaaaaa caggtctgcc ttctttttta aaacaagtgg cagtagacga gtcggataag
     1801 gaaaaaacaa ttgctttttt ccaagattct gtgtcgcctt tattacaaaa ctttatccag
     1861 gttctggcct acaatcacag agcaaatctt ttttatgatg tgcttgtaga ttgcttgaac
     1921 cgacttgaaa aagaaacaaa tcgatttgaa gtgacgatta cgtctgctca tcctctaact
     1981 gatgaacaga agactcgttt gctccctttg attgagaaaa aaatgtctct gaaagtaagg
     2041 agtgtaaaag aacaaatcga tgaaagtctc attggtggtt ttgtcatttt tgccaatcac
     2101 aagacaattg atgtgagtat taaacaacaa cttaaagttg ttaaagaaaa tttgaaatag
     2161 aaagtggtgt tcttttggca attaacgcac aagaaatcag cgctttaatt aagcaacaaa
     2221 ttgaaaattt caaacccaat tttgatgtga ctgaaacagg tgttgtaacc tatatcgggg
     2281 acggtatcgc gcgtgctcat ggccttgaaa atgtcatgag tggagagttg ttgaattttg
     2341 aaaacggctc ttatggtatg gctcaaaact tggagtcaac agacgttggt attatcatcc
     2401 taggtgactt tacagatatc cgtgaaggcg atacaatccg ccgtacaggg aaaatcatgg
     2461 aagtccctgt aggtgaaagt ctgattggtc gtgttgtgga tccgcttggt cgtccagttg
     2521 acggtcttgg agaaatccac actgataaaa ctcgtccagt agaagcacca gctcctggtg
     2581 ttatgcaacg taagtctgtt tcagaaccat tgcaaactgg tttgaaagct attgacgccc
     2641 ttgtaccgat tggtcgtggt caacgtgagt tgattatcgg tgaccgtcag acagggaaaa
     2701 caaccattgc gattgataca atcttgaacc aaaaagatca agatatgatc tgtatctacg
     2761 tcgcgattgg acaaaaagaa tcaacagttc gtacgcaagt agaaacactt cgtcagtacg
     2821 gtgccttgga ctacacaatc gttgtgacag cctctgcttc acaaccatct ccattgctct
     2881 tcctagctcc ttatactggg gttgctatgg cggaagaatt tatgtatcaa ggtaagcatg
     2941 ttttgattgt atacgatgat ctatcaaaac aagcggtagc ttatcgtgaa ctatcgctct
     3001 tgcttcgtcg tcctccaggt cgtgaagcct tcccagggga tgttttctat ctccacagcc
     3061 gtttgcttga gcgctcagct aaagtttctg atgaacttgg tggtggatca attacagccc
     3121 taccatttat cgagacacaa gcaggagata tctcagccta tatcgcaacc aacgtgattt
     3181 ctatcactga tggacaaatc ttccttggcg atggcctctt caatgcaggt attcgtccag
     3241 ccatcgatgc gggttcatct gtatctcgtg taggtggttc tgcacaaatc aaagccatga
     3301 agaaggttgc tggtacactt cgtatcgacc ttgcttcata ccgtgagttg gaagccttta
     3361 ctaagtttgg ttctgacttg gacgcagcaa cacaggctaa gttgaaccgt ggacgtcgta
     3421 ccgttgaggt cttgaaacaa cctgttcaca aaccattacc tgttgagaaa caagtaacca
     3481 ttctttatgc tttgacacat ggtttcttgg atactgttcc agtagatgat attgttcgtt
     3541 tcgaggaaga gttccatgcc ttctttgatg ctcaacatcc agagattttg gaaaccattc
     3601 gtgatacaaa agacttgcca gaagaagcag tcttggatgc tgcgattaca gagtttctca
     3661 atcaatctag cttccaataa gaatagaggt gtcagatggc agtgtctcta aatgatatta
     3721 aaacaaaaat cgcctcaaca aaaaatacga gtcaaatcac taatgccatg caaatggtat
     3781 cggctgctaa gttaggtcgc tctgaagaag ctgctcgcaa cttccaagtt tacgctcaga
     3841 aagtgcgtaa acttttgaca gatatccttc atggtaatgg agctggtgct tcaactaatc
     3901 cgatgttgat tagccgttct gtgaagaaga caggctatat cgttatcact tcagaccgcg
     3961 gtttggttgg aggttataat tcctctattt tgaaagctgt tatggagttg aaagaagaat
     4021 accacccaga cggtaaaggt tttgaaatga tctgtatcgg tgggatggga gctgatttct
     4081 ttaaggctcg cggtattcaa ccactttatg aattacgtgg cttggcagac caacctagct
     4141 ttgatcaagt tcgtaagatt atttcaaaaa ctgttgaaat gtaccaaaat gaactctttg
     4201 atgagcttta tgtttgctac aaccaccatg tcaatacgct aaccagtcaa atgcgtgtgg
     4261 aacaaatgct tccgattgtt gacttggatc caaatgaagc ggatgaagag tacagcttga
     4321 cttttgaatt ggaaaccagc cgagaagaaa ttctggagca gttgttgcct cagtttgcag
     4381 aaagtatgat ttacggtgcc attatcgatg ccaagacagc tgagaatgct gcgggtatga
     4441 cagccatgca aacagcgaca gataatgcta agaaagtcat caatgatttg acaattcagt
     4501 ataaccgtgc cagacaggcg gcgattacac aagaaattac agaaatcgta gcaggtgcta
     4561 gtgccttaga ataggctcta gtccagctcg tatgaaaatg aacttaggac ctagttgagc
     4621 taggaaccga cagtatctta tatagaatag gagaaggaga tgagttcagg taaaattgct
     4681 caggttatcg gtcccgttgt agacgttttg tttgcagcag gggaaaaact tcctgagatt
     4741 aacaatgcac ttgtcgtcta caaaaatgac gaaagaaaaa caaaaatcgt ccttgaagta
     4801 gccttggagt taggagatgg tatggttcgt actatcgcca tggaatcaac agatgggttg
     4861 actcgtggaa tggaagtatt ggacacaggt cgtccaatct ctgtaccagt aggtaaagaa
     4921 actttgggac gtgtcttcaa cgttttggga gataccattg atttggaagc tccttttaca
     4981 gaagacgcag agcgtcagcc aattcataaa aaagctccaa cttttgatga gttgtctacc
     5041 tcttctgaaa tccttgaaac agggatcaag gttattgacc ttcttgcccc ttaccttaaa
     5101 ggtggtaaag ttggactttt cggtggtgcc ggagttggta aaaccgtctt aatccaagaa
     5161 ttgattcaca acattgccca agagcacggt ggtatttcag tatttactgg tgttggggaa
     5221 cgtactcgtg aggggaatga cctttactgg gaaatgaaag aatcaggcgt tatcgagaaa
     5281 acagccatgg tctttggtca gatgaatgag ccaccaggag cacgtatgcg tgttgccctt
     5341 actggtttga caatcgctga atacttccgt gatgtggaag gccaagacgt gcttctcttt
     5401 atcgataata tcttccgttt cactcaggct ggttcagaag tatctgccct tttgggtcgt
     5461 atgccatcag ccgttggtta ccaaccaaca cttgctacgg aaatgggtca attgcaagaa
     5521 cgtatcacat caaccaagaa gggttctgta acctctatcc aggctatcta tgtgccagcg
     5581 gatgactata ctgacccagc gccagcaaca gccttcgctc acttggattc aacaacaaac
     5641 ttggaacgta aattggtaca attgggtatc tacccagccg ttgacccact tgcttcaagc
     5701 tcacgtgcct tggcacctga aatcgttgga gaagagcact atgcagttgc tgctgaagta
     5761 aaacgtgtcc ttcaacgtta ccatgaattg caagatatca ttgctatcct tggtatggat
     5821 gagctttctg atgaagaaaa gaccttggtt gctcgcgccc gtcgtatcca gttcttcttg
     5881 tcacaaaact tcaacgttgc ggaacaattt actggtcagc caggttctta tgttccagtt
     5941 gctgaaactg tacgtggctt taaggaaatc cttgatggta aatacgacca cttgccagaa
     6001 gatgccttcc gtggtgtagg ttctatcgaa gatgtgattg caaaagctga aaaaatggga
     6061 ttttaagagg tgacctatgg ctcagttaac tgtccagatc gtgacaccag atggtctcgt
     6121 ctatgatcac catgccagct atgtatcggt tcgaactctg gatggtgaga tggggatctt
     6181 gccacgacat gaaaatatga ttgcggtttt agcagttgat gaagtaaagg taaaacgtat
     6241 cgatgataaa gatcacgtga actggattgc agtaaacggt ggcgttattg aaattgccaa
     6301 tgatatgatc acaatcgtcg ctgactctgc agaacgtgct cgtgatatcg atattagccg
     6361 tgcagaacgt gccaaacttc gtgcagaacg tgcaattgaa gaagcacaag acaaacattt
     6421 gattgaccaa gaacgtcgtg ctaagattgc tttgcaacgt gccattaacc gtattaatgt
     6481 cggaaataga ctataagaaa aaatgaactt gaaaatacca agttcatttt ttaggctctt
     6541 tgtcaactgt agtgggttga aaaa
//
LOCUS       DAVMMR010000081         6523 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMMR010000081 DAVMMR010000000
VERSION     DAVMMR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.081"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..102
                     /locus_tag="VYS74_001863"
     CDS             <1..102
                     /locus_tag="VYS74_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV3789855.1"
                     /translation="IEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            188..1138
                     /locus_tag="VYS74_001864"
     CDS             188..1138
                     /locus_tag="VYS74_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV3789856.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPISLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1315..2322
                     /gene="gap"
                     /locus_tag="VYS74_001865"
     CDS             1315..2322
                     /gene="gap"
                     /locus_tag="VYS74_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV3789857.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2389..3225)
                     /locus_tag="VYS74_001866"
     CDS             complement(2389..3225)
                     /locus_tag="VYS74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3789858.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3351..5546
                     /locus_tag="VYS74_001867"
     CDS             3351..5546
                     /locus_tag="VYS74_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3789859.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5599..5751
                     /gene="rpmG"
                     /locus_tag="VYS74_001868"
     CDS             5599..5751
                     /gene="rpmG"
                     /locus_tag="VYS74_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3789860.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5761..5937
                     /gene="secE"
                     /locus_tag="VYS74_001869"
     CDS             5761..5937
                     /gene="secE"
                     /locus_tag="VYS74_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV3789861.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5992..>6523
                     /gene="nusG"
                     /locus_tag="VYS74_001870"
     CDS             5992..>6523
                     /gene="nusG"
                     /locus_tag="VYS74_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV3789862.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2035 a         1136 c         1411 g         1941 t
ORIGIN      
        1 attgagaaaa catgggctca tatcaaaaag cacctcaaaa aggtattacc aagttgcaat
       61 accttttatg aggctctttt gtcctgctct tgtttcaatt gactatagtt cacggataca
      121 gttgggaaag aagctattaa atatttggag agtccttttg ggatgaacat gtcaaatatt
      181 actatagatg ggcaagaaaa gatgggcaag aaatggatct gagtcaaacg atgcttctta
      241 tgctcaagta gtgagtcttt atgatgatac aagtatctct gttagtaata atgagacgga
      301 caaagtgttg actggatccc tctatactga aacaaatgaa cagggattaa cgattccaat
      361 cagtttacta aaaaattgga atgaacagac aggaaaaaat ttgacaacta atgatcttat
      421 tggcaaatca gtctcagcca gcattgtaga aagtgctgcc gaaactagta agattgctca
      481 atttcaaacg aagattgtac gtgtaatcaa tgatgaagat gacatggagg acagcaacag
      541 tttcatgctg tctcatcaaa tggaaacgat tttgaaagag gctggattta cgaaagctgt
      601 atcttatttt atcttggaac tcaaagatcc atcacagaca aaagtagtaa cagaagaatt
      661 acagaaaaat aagaagtata ctgtgctttc tcaacagaga gttcttgata ttgtgattac
      721 ctttattcgt gttattcagg gattattgat tgtgctttca tcacaagcta ttgtggtagc
      781 agcggttatg attggtatca ttatttacat caatatcatg caacgttcca aggaaatagg
      841 tgtcatgaaa gcagttggtt atcagaatcg tggtgtcaaa ggaattttta tttacgaggc
      901 tatctggatt gtaggcatcg ccttgctgct ggcatttttg gttgcacaag gggtgggaag
      961 tttggcgaat gcgattgtaa gtcactttta cccatccatc actaaggttt ttgaattaaa
     1021 tcttttatct gttttaggaa ctctagtttt cgctctatta cttggttatg tctcagccta
     1081 cttcccggcg cgtaagatta gtaaaatgga tcctgtagaa tcgttacgct atgaataata
     1141 agggattcct tgatttacag tcttaaaaag aaactcacat agaaaaacaa tggccatact
     1201 tgcaattcaa ctcaaatagt tgtataatag gtttgttgaa aggtgatttg tagtgattca
     1261 agttactctt ttcacaataa aaatttcatg attttcataa ggaggaaatc actaatggta
     1321 gttaaagttg gtattaacgg tttcggacgt atcggtcgtc ttgctttccg tcgtatccaa
     1381 aacgtagaag gtgttgaagt tacacgcatc aacgacctta cagatccagt tatgcttgca
     1441 cacttgttga aatacgacac aactcaaggt cgtttcgacg gtactgttga agttaaagaa
     1501 ggtggattcg aagttaacgg taaattcgtt aaagtttctg ctgaacgtga tccagagcaa
     1561 atcgactggg ctactgacgg tgtagaaatc gttcttgaag ctactggttt ctttgctaag
     1621 aaagaagcag ctgaaaaaca ccttaaaggt ggagctaaaa aagttgttat cactgctcct
     1681 ggtggaaacg acgttaaaac agttgtattc aacactaacc acgacgttct tgacggtact
     1741 gaaacagtta tctcaggtgc ttcatgtact acaaactgct tggctccaat ggctaaagct
     1801 cttcaagata actttggtgt tgttgaagga ttgatgacta ctatccacgc ttacactggt
     1861 gaccaaatga tccttgacgg accacaccgt ggtggtgacc ttcgccgtgc tcgcgctggt
     1921 gctgcaaaca tcgttcctaa ctcaactggt gctgctaaag ctatcggtct tgtaatccca
     1981 gaattgaacg gtaaacttga cggatctgca caacgcgttc caactccaac tggatcagtt
     2041 actgaattgg tagcagttct tgaaaagaac gttactgttg atgaagtgaa cgcagctatg
     2101 aaagcagctt caaacgaatc atacggttac acagaagatc caatcgtatc ttcagatatc
     2161 gtaggtatgt cttacggttc attgtttgac gcaactcaaa ctaaagttct tgacgttgac
     2221 ggtaaacaat tggttaaagt tgtatcatgg tacgacaacg aaatgtcata cactgcacaa
     2281 cttgttcgta ctcttgaata cttcgcaaaa attgctaaat aattcttgag ttgatagaaa
     2341 gcaaggcttt gtggtcttgc tttttatata gaaaaatgga tgacacgatc atccattctt
     2401 ttttaattct ttttcaaatg tatttgaaag ggtagtgaaa gttagctttt ctaaagtaag
     2461 tgggtgggta aaggaaagtc ggaaggcatg aagcataagc cgacttgtct ttgatttact
     2521 attatagaga gggtcgccca ggataggaag attatgatgc gaaaggtgca cacgaatctg
     2581 atgggttcgc cctgtcttta gcttgcaacg agccaaggaa gtcttgtttg agaattgttt
     2641 taatctgctt acatacgttt cagcatattg cccatttttt gcatcaacta ttctttttct
     2701 acgatcatgg cgatcacgtc caattttgtc tctgaaaaca agttcttttc tgttgatatt
     2761 tccatcaact agagcccaat attctctaga aatctctttt ttctccaata agcgattgag
     2821 aatgggcagg ataaaaggat ttttggcaaa gagaactaag ccactggttt ccatgtccag
     2881 acgatgaacg acatagcagg tttggccaac ataggtactg acatggttaa gaagggcaat
     2941 ttcgtttggt tgattaccat gcgttttcat cccctctggt ttgtttacaa taatcaagtg
     3001 ttgatcttga taaacttcct gcactaagtc tgggttgccc caagggatcg tcttttgggg
     3061 ataatcttcc tcgtcaaaag tcaactggca aacatctcca ggatttacga tttcgttcca
     3121 gtggacttct tcttgattta tcaaaatatg tttcttgatt ctcaaaaaat gacggatttt
     3181 tctagggatg aggagttgtt cctcaagtaa ttgctttacc gtcatttgag gtatagaggc
     3241 gggtaatgta aatgtgaatt gcatacagat attgtaacaa aaaaagccct atttggatag
     3301 gaaatagcta aattcttgtc ttcctatgat gaagatgata aaataaacgc atgaaattag
     3361 ataaattatt tgagaaattt ctttctcttt ttaaaaaaga aacaagtgaa ctagaggact
     3421 ctgattctac tatcttacgt cgctctcgta gtgatcgaaa aaaattagcc caagtaggtc
     3481 cgattcgaaa attctggcgt cgttatcatc taacaaagat tatccttata ctaggtttga
     3541 gtgcaggctt gctagttgga atctatttgt ttgctgtagc caagtcgacc aatgtcaatg
     3601 atttgcaaaa tgccttgaaa actcggactc ttatttttga ccgtgaagaa aaagaggctg
     3661 gtgccttgtc tggtcaaaag ggaacctatg ttgagctgac tgacatcagt aaaaacttgc
     3721 agaatgctgt tattgcgaca gaagaccgtt ctttctataa aaatgacggg attaactatg
     3781 gccgtttctt cttggctatt gtcactgctg gacgttcagg tggtggctct accattaccc
     3841 aacagctggc taaaaacgcc tatttatcgc aggatcaaac tgttgagaga aaagcgaaag
     3901 aatttttcct tgccttagaa ttaagcaaaa aatatagtaa ggagcaaatt ctaaccatgt
     3961 accttaacaa cgcttatttt ggaaatggtg tgtggggtgt agaagatgcg agtaagaaat
     4021 actttggagt ttctgcatca gaagtgagtc tggatcaagc tgcgactctg gcagggatgc
     4081 tcaaggggcc ggaactgtat aatcccttga attccgtaga agattctact aatcggcgcg
     4141 atactgtctt gcagaatatg gttgcagcag gatatattga taaaaaccaa gaaaccgaag
     4201 ctgctgaagt tgatatgact tcgcaattgc acgataagta tgaaggaaaa atctcagatt
     4261 accgttatcc ctcttatttt gatgcggtgg ttaatgaagc tgtttccaag tataatctaa
     4321 cagaggaaga gattgtcaat aatggctacc gcatttacac agagctggac caaaactacc
     4381 aagcaaatat gcagattgtt tatgaaaaca catcgctatt tccgagggca gaggatggaa
     4441 cgtttgctca atcaggaagt gtagctctcg aaccgaaaac agggggagtt cgtggagttg
     4501 tcggtcaagt tgctgacaat gataaaactg gattccggaa tttcaactat gcaacccaat
     4561 caaagcgtag tcctggttct acaattaagc ctttagttgt ttatacacca gcagttgaag
     4621 caggctgggc tttgaataag cagttggata accataccat gcagtatgat agctataagg
     4681 ttgataacta tgcagggatc aaaacaagtc gagaagttcc tatgtatcaa tccttggcag
     4741 aatcgcttaa tctacctgct gttgccactg ttaatgattt gggtgttgac aaggcttttg
     4801 aggcaggcga aaaattcgga ctcaacatgg aaaaggtcga ccgtgttctt ggtgtcgcct
     4861 tgggaagcgg tgttgaaacc aaccctcttc aaatggctca agcatacgct gcctttgcaa
     4921 atgaaggttt aatgcctgaa gctcatttta ttagtagaat tgaaaatgct agtggacaag
     4981 ttattgcgag tcataaaaat tcacaaaaac gggtgattga taagtctgta gctgacaaga
     5041 tgaccagtat gatgttgggg actttcacca acggtaccgg tattagttca tcgcctgcag
     5101 actatgtcat ggcagggaaa actggaacaa ctgaagcagt tttcaatccg gagtacacaa
     5161 gtgaccagtg ggtaattggt tatactccgg atgtagtgat tagccactgg cttggctttc
     5221 cgaccactga tgaaaatcac tatctagctg gctctacttc aaacggtgca gctcatgtct
     5281 ttagaaacat tgccaatacc attttacctt atacgccagg aagtaccttt acggttgaaa
     5341 atgcttataa gcaaaatgga attgcaccag ccaatacaaa aagacaagta caaaccaatg
     5401 ataatagcca gacagatgat aatttgtctg atattcgagg gcgtgcgcaa agtctagtag
     5461 atgaggctag ccgggctatc tcagatgcga agattaagga aaaggctcaa acaatatggg
     5521 attcgatagt caatctattt cgctaagatg cttgtcaaag cctagctttc ttgttataat
     5581 ggataagatg gaggcgttat ggcactaaaa aaagcaagcc tagcttgtgc ggtttgtggt
     5641 tcgagaaact attcaatcaa gatcagcgga aaccccaagc ctacacgact agaagtaaat
     5701 aaattttgta agcattgtgg caagtacact acacacagag aaacgagata ggagagagcg
     5761 atgcgtttta ttggagatat ttttagactt cttaaagaca caacatggcc aactcgcaag
     5821 gaaagctgga gagattttcg ttctatcatg gaatacacag ctttctttgt agtaattatt
     5881 tacatttttg accagttgat tgtttcaggt ttgattcgat ttattaacat tttttagaag
     5941 attagtggag ttaattacac tagaaatctt ctatttatga aaggaaatat catggatagt
     6001 tttgataaag gatggtttgt tttacaaact tattctggtt atgaaaataa ggtgaaagaa
     6061 aatctattac aacgtgcaca aacctacaat atgttggata atattctacg cgttgaaatt
     6121 ccaacacaaa cagtgcaagt tgaaaaaaat ggaaagagaa aagaagtaga agaaaatcgc
     6181 tttccaggtt atgttcttgt agaaatggtc atgacagatg aagcttggtt tgttgttcga
     6241 aacacaccaa atgttacagg atttgtcgga tcacacggga acagatcaaa accaactcca
     6301 ttattggaac aagaaattcg tgacattttg gtatctatgg gacaaactgt tcaagaattt
     6361 gatttcgatg ttgagattgg tcaaactgta cgtattattg atggtgcttt tgcagactac
     6421 actggtaaga ttacagaaat tgataataac aaagtgaaaa tgattatctc tatgtttggt
     6481 aatgacacag ttgcagaagt aaacctaaac caaatcgcag aat
//
LOCUS       DAVMMR010000082         6389 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMMR010000082 DAVMMR010000000
VERSION     DAVMMR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.082"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..299)
                     /locus_tag="VYS74_001871"
     CDS             complement(<1..299)
                     /locus_tag="VYS74_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789863.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAI"
     gene            complement(296..649)
                     /locus_tag="VYS74_001872"
     CDS             complement(296..649)
                     /locus_tag="VYS74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV3789864.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGDYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(705..1607)
                     /locus_tag="VYS74_001873"
     CDS             complement(705..1607)
                     /locus_tag="VYS74_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789865.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLMYEMSISNNNNWQEKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(1815..2189)
                     /locus_tag="VYS74_001874"
     CDS             complement(1815..2189)
                     /locus_tag="VYS74_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV3789866.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            complement(2565..3542)
                     /locus_tag="VYS74_001875"
     CDS             complement(2565..3542)
                     /locus_tag="VYS74_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789867.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
     gene            complement(3856..4914)
                     /locus_tag="VYS74_001876"
     CDS             complement(3856..4914)
                     /locus_tag="VYS74_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789868.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            complement(5795..6007)
                     /locus_tag="VYS74_001877"
     CDS             complement(5795..6007)
                     /locus_tag="VYS74_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789869.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            complement(6161..>6389)
                     /locus_tag="VYS74_001878"
     CDS             complement(6161..>6389)
                     /locus_tag="VYS74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789870.1"
                     /translation="DTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQK
                     AGYLFSILLLFSNFSVPFIKEKEYELFKNKK"
BASE COUNT         2347 a         1114 c          803 g         2125 t
ORIGIN      
        1 aagattgcta actctaggat aaaaacactt attaatacgg tataagtcaa aaaactttct
       61 gggaaaaact tattgattaa aatggggaaa ctcattaaca gaatcgaatt tatggctaga
      121 cagatagata ctattttatt tttcacagaa tatagaggta gtgatactat tgagtagata
      181 aaagctaaaa aaataagcaa aaaatactcc gtgtcattta taatcgccct gaatgataaa
      241 aatatataca tccaacatac gataatatgt atttttcgaa tcttatctac attattcatc
      301 ttactgtcgc agatatgaat ccatcccaag gataagggtt cccgctaata tcacaacctc
      361 tattaagagc accatatcca atacagtaag ctattttccc tgctgctgta gccaatacac
      421 ctgctgctgc accaataagc cagcctccaa gacctccaac cttaatatag tttattagtt
      481 ttttggttaa aactgcagct ccagctaatg ctataaaagt tcctggactc atagctaaag
      541 ctgcgcaaat tccttgacaa gcacgcttgc ttaaataatc tcccccatca agatacatca
      601 ttacttcttc atcaatcaca acataattat ttggtaatac aagttccatt tgttttctcc
      661 tccttagttg aaattgtttc atttgtttta caaataggac gaacttaaac tatcttttta
      721 ttactcagta ataatttccg agatttcatt ttatgatact ctctattcat acagaaatca
      781 aaattttgcc ttaaccaact tgtataatag aaaaaatcta attgattgat tctgatgagg
      841 tgtatcctaa ttttatcaac cttttcttgc cagttgttat tgttactaat agacatttca
      901 tacatcagta actcgacaag acctgtacac agaagggtgt tatgagaatc ttcgattttt
      961 ttcaaaatat cctcaaaaag ttcactatcc gttaaaaagg tggattggtt catcgcatca
     1021 tacagctgaa gagacattaa gtcagaataa cttagctctg atttttgtat cttatccttt
     1081 aacgttttcg tcaaatcgct ttcaaacgaa tcttcttttt cccatttatt ttgattaaaa
     1141 agaaattccg tcttattaaa aacgagatga ttgatatcca ttatagtcca gaaaaatatt
     1201 gtagtaagca tgatgattgc aaaaggaatt tttaaaataa atactaggat aatagcaatt
     1261 tcaagaataa aactccatat aatcctagat cttaaatcat caggcgacaa acttttacga
     1321 tggaatgaaa aatagggcga gaatctcaca ttcacttttt tccttcttat aaaaataaag
     1381 ggaaaaatct taataagacc ttctttcttg tataaatctt tcaatgcgta tagtatgtct
     1441 aaaactattt ttgataaaat caaactacag aaagcaagac tcatactaat cacaaaatca
     1501 aggggagatt ctagattcaa tagtaagtta ttcactagga catgcaactc tagccacaag
     1561 acaaaaagag ggagcattag taagaaaaca attagtttac gtatcataat tttacagtgt
     1621 ccttatcatt ttcttaattt tggataaatc atcattgcta tgattaaaat caaaaaaacg
     1681 acattcatcc aaataatcaa cttttttatt tcttcgctaa caaaatcata ggctaaaaaa
     1741 gttatggatg atataaatag acatacaaaa aaaataataa caaaaatttt ttccatttta
     1801 atacccatat ctagttacca caaggcgttt aaatttgggt ctctcctatt tacatcattg
     1861 ttggcccaga ttgttgtaat agcatcgcca attgacatcc aagctgcaaa tccattaata
     1921 gcagacagtg caaatcctgc cactctattt gcaactcgta ccccaacata tcttgcagct
     1981 gctccagcaa ttgcgcttgt aactcgccgc aaaccataat tacgaatata atttctaact
     2041 agactgattg ctcctccacc taaaacacca ttaaaagcta tattaatggc tgttgcaaca
     2101 ggccatctcg gaatagaaaa tcccccatca agatacatca tctcttcttg ctcaagaaca
     2161 acataattat ttggtaatac gagttccatt tattttctcc ttttttatta agtatggtta
     2221 ggatggtttc ctaacaagta atcgtcgact gtgtttttat cacttagcta cctagataag
     2281 atatcctttc tctaccttat ttgtttttat aggttaccta aggtttaaaa ccaattattg
     2341 tttgaaactt aacagttcta aaccactagc aataaatgat tgcgaactca ctaatactat
     2401 ttcaaaattt atttttttga cttagtaacg caattccaat tagcaataca cctattataa
     2461 ataaaagtat ttcccaaaat ttattatccg tgttaatgag aggtataaga tacatactga
     2521 ctaatatgca acttgcccct ataatagcaa atgttattct agttttatct cctaattgaa
     2581 acatgtttga tccctatata cacttcaagc aaaagttgga tttctgaaat agctaaagat
     2641 aaagataatg gttgcgagct atttaaacta tcatcaatat tctgctcgat taaaacaaag
     2701 tctggtctta cctctaaaat tttatttaac attgattcat atttattttt ttcattataa
     2761 tgccgtaaat atagatttaa ttttataaca tccaacatac cataaagtga tattggatga
     2821 tttatttttt taaatcgctt aacttctaat aaatcaattt gttcacgcac gctctctgaa
     2881 agatatggct tgatcaaact gatataagat aaagccacgc atggcaactc agattttgat
     2941 tgcaatttcc aaatttcatg ataatcctga agattaaact cacaattctt cttagattcc
     3001 agtattgtta gtacctttga ttttaacaga aataattgtt gattctcaat tctataaagt
     3061 ttttgtacta atgctatgat aatagaaaat gaaataagag gcaaaagcaa cggtttgtca
     3121 tacacaaaag taaagaaaaa atagtatgaa attagtgttg taaagtacaa gaagtagtaa
     3181 aatttaaaca gattcttagt tgtttgactc tccttataaa acactgctat tcctggacta
     3241 aatcccatta gttggtgaaa gagaattcga tagtatttcc tagctcctat aatgatcaca
     3301 aatataaaat aaaaataaat tggtcttaat ttacaaaaga ttgaggaaac tagtaatagc
     3361 aaagtatgta agcattgaga caaaatatag aataacggta tcgccaatac aaaggttaag
     3421 atggcatgag agggagttcc taagatgtcc aattttataa atactgggct gacacaaaat
     3481 atcgtgctta taagcagtaa aattattaaa attgtgtcaa aaaaataacg cttatttttc
     3541 attagtctac tttccattgt taaataatac tactgctaac accaagccaa cttagcacat
     3601 tgcatcacct aatcctgcgc tacttgtcca taatccttta atgtttctac taaaattatt
     3661 ccaatttccc ccatcaagat agcttattta tcctagacaa tgcctactgc aacagtataa
     3721 ataagattca tcagccatct tttctggcaa tcatatgggt agcctaatga tatttctagt
     3781 tactcaaaaa tatctagcta tactagagtt atttctaatc cctaggacac ttgttaatcc
     3841 aaatgtaaca aattttcacc ccttaatagg actctttctg gaaaaaatat aaggtctatc
     3901 tttataaaaa aatctatcat attgggatgg cacgcatctg gaatttaaac aagctataaa
     3961 ttcatttata actactattt gtttagaggc aacagcatct tgtataccgc cctcgaaaga
     4021 accatcttgt aattccgaaa tatttttgtt taaatattgt tttgacaaat ctcttaatgc
     4081 ttcattatcg taaatgattc ccaaaaatcc aaccttatat agtaaataat ttaaacttac
     4141 aaagtaattg aatcccatag tatatttttc tttataaagc aaaggtaaaa ctttataaaa
     4201 aatatctaag tcaacctcac tctgattctg aagcaagtag gagtcaagca aattttcaaa
     4261 aatagctaac aatatgattg gtgtaaagtt tttgtattca catgtcaagt attctgagta
     4321 tcgtgcagat gatatttctt ttatataact ttttgaaagc aagatttttt caaattcatc
     4381 atctattatt aatcttctat ttccaatcca agcggtatta ccacccaaat aagattgtgc
     4441 aaatagcaat atgtaagaaa atatgaacgt aaatactgaa agaggtagaa ttctatgcgt
     4501 aaaaaaatag acgataatgg tagccaggag taaagatatc tctcgtgaca atcgaattct
     4561 tttcagcatc ttttttaaca gtccgtaagg ttgtccttgg caatattctt gaactaaatt
     4621 aatcggaatg acatctctca ccatttctgg aaagtagagt aagtttatag gttgaaatct
     4681 ccaactccca tcataagtaa atggatatag aaccaaatac ttaaaagatt tttttcttat
     4741 aaacttaatt atcacaatat gaatgactgt tgcaatgaag tatattaaat ataaaagtaa
     4801 aaccgccatc aatatactta caaaaatccc cttggactca atcaatggaa aataaaatat
     4861 tgcaactaga agcgaataaa gtcccgctaa taaatatttc tgatcatctt tcattcaaaa
     4921 caccatatta tttactaaga atacatcaat acaatagagc aactctgtaa gtcaatattt
     4981 gataaagcaa agctttaacc tcttccccag acgttagctt ttcttcaacc cactacagtt
     5041 gacaaggagc ccaaattatt gataaaaact ttagtcatca tcgtctctat aaaaatattt
     5101 tctatacact cttgataata ccaaaattaa tgccaatatc ataccaagag ctataataat
     5161 agaatttatt agatttattt tagtatatcc aatcactagt ccaataattg gactagcaga
     5221 catgcttaga tataataaaa ataacaaaaa atgaatttca ccaattcctg caaaaaaatg
     5281 tttcatagct atagttttat gcccataaat acccatcaaa tttaccgtct acgatatcta
     5341 aaccatatgc aatggctcct cctaatgaga agacttcagc aacagtagtc gcaatattaa
     5401 gagcattagt aatagttgcc atactaactc caccaatata tcttgttata gcacttagtt
     5461 acctagataa gatatccttt agcttgcttc caaaatttaa gtctacacct ataatatgag
     5521 ttcatattag tactgctaaa tcctatctat agtaatgtca aacacctaaa taaagtcatc
     5581 tctccaattc attcaagaaa tgaagaaagg caaaaaatct ttaaaataag aaaagtgcat
     5641 attatcaggt gttcaaaaat cttgataata tgcgttatat aatagaaaaa tttactcaat
     5701 ttcattttca aattgagttt tttcccagcc tcatttttaa tttatatctt caatagaatc
     5761 taatgaccga gctactgtat tacacagatt aatattattt agtctgtaat ttttttaaaa
     5821 aacttaatag taaatttgct ccactgattg ctatcaatat ttcaattggt aaaaaatagt
     5881 aattccaaat aaaatgagaa atcagcaatg atagaaaaat aacaatatca attttcctgt
     5941 tgttgacata ctctgtaatt ttatgataca ggaatgcaac aataactgaa attaaaacaa
     6001 ccatcataat actattatcc tatccttctc actaactcta tttccaaaat ttaatttttg
     6061 ggggacttag taatgaaatt acaattaaca acacacctaa aataaataaa aatatctccc
     6121 aaactttatt ctcagtttta ataaagctac catatctatc tcatttctta tttttaaata
     6181 attcgtattc cttttctttg ataaatggaa ctgaaaaatt agagaaaaga agtaaaatcg
     6241 aaaacagata tcctgctttt tgaatcacta aaagatatcc taaaaataag taagctataa
     6301 catccaatgc gaaaagaaaa aatattctcc tcttcattaa agtacttgtg tcaaataatc
     6361 tgatgaataa aaattttatc gtggtatct
//
LOCUS       DAVMMR010000083         6211 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMMR010000083 DAVMMR010000000
VERSION     DAVMMR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.083"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            1..192
                     /locus_tag="VYS74_001879"
                     /pseudo
     CDS             1..192
                     /locus_tag="VYS74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            347..547
                     /locus_tag="VYS74_001880"
                     /pseudo
     CDS             347..547
                     /locus_tag="VYS74_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            572..988
                     /locus_tag="VYS74_001881"
     CDS             572..988
                     /locus_tag="VYS74_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789871.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1219..1926
                     /gene="ccdA2"
                     /locus_tag="VYS74_001882"
     CDS             1219..1926
                     /gene="ccdA2"
                     /locus_tag="VYS74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3789872.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1937..2494
                     /gene="sdbB"
                     /locus_tag="VYS74_001883"
     CDS             1937..2494
                     /gene="sdbB"
                     /locus_tag="VYS74_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV3789873.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDDKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2868..4259
                     /locus_tag="VYS74_001884"
     CDS             2868..4259
                     /locus_tag="VYS74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV3789874.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4402..5319
                     /gene="adcAII"
                     /locus_tag="VYS74_001885"
     CDS             4402..5319
                     /gene="adcAII"
                     /locus_tag="VYS74_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV3789875.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDREHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5327..>6211
                     /gene="phtB"
                     /locus_tag="VYS74_001886"
     CDS             5327..>6211
                     /gene="phtB"
                     /locus_tag="VYS74_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV3789876.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGL"
BASE COUNT         1898 a         1113 c         1324 g         1876 t
ORIGIN      
        1 taagaggcaa agtatctgga agaagatatc agaggatttc tttggttgca ggtctaacaa
       61 atgaatggtg agttaatcgc tccaatggct tacgaagaga cgatgacgag cgactttttt
      121 gaagcttggt ttcagaagtt tctcttacca acattaacca caccatcggt tattatagta
      181 aaacgaaata agaataggac aaatcgatca ggacagtaaa atcgaattct aacaatgttt
      241 tagaagtcca tatgtactat tctagtttca atataccata actgctccta gtcaacctgc
      301 aagtcctgcc ccagctccaa gctcagctcc gactcaagat cctagagtgg tagtaagcgc
      361 ttcggctagc tcaacgagca cacaagctca agagtaagtt gacaagtctg aacttcgtgc
      421 cttgagtcaa gagttagacc aacgcttgaa agctttggca acagtatctg atccgaaaat
      481 cgatgcaacc aaggctgtcc tcctagatgc tcaaaaagct ccggaagata gtgccttgac
      541 agagtaaggt ctgcatactg cagtagagtc tgtgaaggct gccttggact ctctcaaaga
      601 tgtgaaagca aatgctagcg acagcaagcc tgcacaggac aagaaggatg caaaacaagg
      661 aacggaagat agtaaggatt cagataagat gactgaaaca aactcagttc cggcaggagt
      721 gattgtggtc agtctacttg ccctcctagg cgtgattgcc ttctggctga ttcgccgtaa
      781 gaaagagtca gaaatccagc aattaagcac ggaattgatc aaggttctag gacagctaga
      841 tgcagaaaaa gcggataaaa aagtccttgc caaagcccaa aaccttctcc aagaaaccct
      901 tgatttcgtg aaagaagaaa atggctcagc agagacagaa actaaactag tagaggagct
      961 taaagcaatc cttgacaaac tcaagtaaaa catgatttat gaaatgtcag acctaaaaga
     1021 cattttcgtt cacaaaagca gtgacgagta atccttgtcg ttgctttttg gctattttgg
     1081 gggataagat ccgccttgac tagcaaaaga aaagattggt ccaatagtgc ccgatacgat
     1141 tttgcgtagc agaaagctta caaataaaat tgaatatagt aaaataggat tagaattatt
     1201 aagaaagttg gttctttatt ggaaacgata gtatttttaa tctctgtttt tctagcaggt
     1261 gttttatcct ttttttctcc ttgtattttt cctcttctgc cagtctatgc tgggatttta
     1321 ttggatgatc aagaaagtgc aaaaagcttt tctttgtttg ggagaaaggt tctctggtca
     1381 ggcttgattc gaacactttg ctttatcgct ggtatctctc tcattttctt tattctaggc
     1441 tttggtgctg gttactttgg tcatattctc tatgcaaatt ggtttcgata tgtcatggga
     1501 gctattatta tcattttggg tcttcaccag atggaaattt ttcatttgaa gaaattagaa
     1561 gttcaaaaaa gttttacctt taaaaaatca gattctaatc gttattggtc agctttttta
     1621 cttggtatta cctttagctt tggttggaca ccttgtattg gtccagtttt aagttctgtt
     1681 ttagcacttg cggcttctgg aggcaatggt gcttggcaag gagcgattta tactctcatt
     1741 tacactctgg gcatggccct tcctttcttg gtcttggcac tagcttcagg tctagtcatg
     1801 ccatatttta gtaaaatcaa gcgtcatatg atgctactaa agaaaattgg tggtttcctc
     1861 atcgttttaa tgggaatttt gttactatta ggacaagtaa atgttctagc tggaattttt
     1921 gaataaagga ggacaaatga aaaaagtaat gtttgctggc ttaagtctct tgtcattagt
     1981 tgtattgatg gcctgtggtg aggaagaaac taaaaagact caagcagcac aacagccaaa
     2041 acaacaaacg actgtacaac aaatttctgt tggaaaagat gctccagact ttacattgca
     2101 atccatggat gacaaagaag ttaagttatc tgattttaag ggtaaaaagg tttacttgaa
     2161 gttttgggct tcatggtgtg gtccatgcaa gaaaagtatg ccagagttga tggaactagc
     2221 ggcgaaacca gatcgtgatt tcgaaattct tactgtcatt gcaccaggaa ttcaaggtga
     2281 aaaaactgtt gagcaattcc cacaatggtt ccaggaacaa ggatataagg atatcccagt
     2341 tctttatgat accaaagcaa ccaccttcca agcttatcaa attcgaagca ttcctacaga
     2401 atatttaatt gatagccaag gaaagattgg aaagattcaa tttggtgcta tcagtaatgc
     2461 ggatgcagaa gcagcattta aagaaatgaa ctagaatcga taaaatctga ttacaagatg
     2521 tagtcagatt ttggtaactt ctaaaactaa cttccagttt cccacaacct agcttttagt
     2581 atgagaaaaa cgcgtgacat cagtggaaat ccgtcttaga ctaacttcca ctggttttct
     2641 ttttcttgat atataagggt tcaaaagcga aaagactcag aaaaagtgca cgacaaatag
     2701 ccctaaaaca gagtcgtctt agagtaactt ccagttttgg gagagagatg gaagttactt
     2761 tgagaagtta cggtagtcag atttttttag aaaaacattt tataaatatt cacatatctc
     2821 ctatatttat ggtaaaatag aattatcagt ttattttgga gtcaaagatg aatatattta
     2881 gaacaaagaa tgttagttta gataaaacag agatgcatag gcatttgaag ttatgggatt
     2941 tgattttgct gggtatcgga gccatggtag ggacaggcgt ctttacaatc acaggtactg
     3001 cagctgcaac acttgctggc ccagccctag tgatttcaat cgttatttct gccttgtgtg
     3061 tgggattatc agccctcttt tttgcagaat ttgcctcgcg agtacccgct acaggaggtg
     3121 cctatagtta cctctatgct atcttaggag aattccctgc ctggttggct ggttggttaa
     3181 ccatgatgga gttcatgaca gccatatcag gcgtagcttc gggttgggca gcttatttta
     3241 aaggcttgct ctctcaatac gggatagcct ttcctcaggc tttaaatggt acctttaatc
     3301 ctcaagcagg gacatttgtt gatttattgc ctattcttgt cttggtcttg gtgacttcgc
     3361 ttgttttact aaatgccaag gcagccttac gctttaattc gattctagtt attttgaaat
     3421 tttccgcttt agctctcttt gttttagtag gaatttggaa tattaagttt gataattgga
     3481 gcaattttgc tccttacggt tttggacaaa tctatggagc tagtactggt attatggctg
     3541 gtgcatcctt gatgttcttc ggttttttgg gatttgaatc catctctatg gctgtagatg
     3601 aagtcaagac tcctcaaaaa aatattccta gagggattgt cttatcgctt tctatcgtaa
     3661 ccattctcta tgccttggtg acccttgttt tgactggtgt ggttcactat agtcatttaa
     3721 atgtcgatga tgccgttgcc tttgcccttc gtagtgttgg gattagttgg gcagccaact
     3781 atgtgtcatt agtggctatc ttgaccttga ttacagtttg tatttcgatg acctatgccc
     3841 tatcgcgtat gatttacagt ttagcacgtg acggcttagt gcctgctgcc tttaaggaac
     3901 tgacgaagac tagcaagata ccaaagaatg ctactatctt gacaggtcta gcttcagcag
     3961 tagcagcagg aatgttccca ctagccagta ttgcagcctt tttaaatatt tgtaccttag
     4021 cctacttgat catgttggct tatggtctga ttcgcttacg gaaagaaaaa ggaatgccca
     4081 aagctgggga atttaaaaca ccattggtac ctctgttacc aattttatcg atcatcattt
     4141 gtttatcctt catgttacag tataatatga atacctggat tgctttccta gttgcgttgt
     4201 tagtaggaag tattatttac ttcacttatg gctataagca ttctaccata gaagaataaa
     4261 gtgaaagtct attgagttgt tgacactcag tagattttta tatgtgaaag aaatttcaaa
     4321 ttttttctaa aaatatcttg acagattaaa ttttcaggag tagaatattt actagttaat
     4381 taaatggtta aggagttgtt catgaagaaa caaaatttat ttttagtcct gttaagtgtc
     4441 tttcttttgt gcttgggggc ttgtggtcaa aaggaaagtc agacaggaaa ggggatgaaa
     4501 attgtgacca gtttttatcc tatctacgct atggttaagg aagtatctgg tgacttgaat
     4561 gatgttcgga tgattcagtc aagtagtggt attcactcct ttgaaccttc ggcaaatgat
     4621 atcgcagcca tctatgatgc agatgtcttt gtttaccatt ctcatacact cgaatcttgg
     4681 gcaggaagtc tggatccaaa tctaaaaaaa tccaaagtga aggtcttaga ggcttctgag
     4741 ggaatgacct tggaacgtgt ccctggacta gaggatgtgg aagcagggga tggagttgat
     4801 gaaaaaacgc tctatgaccc tcacacatgg ctagatcctg aaaaagctgg agaagaagcc
     4861 caaattatcg ctgataaact ttcagaggtg gatagggagc ataaagagac ttatcaaaaa
     4921 aatgcgcaag cctttatcaa aaaagctcag gaattgacta agaaattcca accaaaattt
     4981 gaaaaagcga ctcagaaaac atttgtaaca caacatacag ccttttctta tctagcgaag
     5041 agatttgggc ttaatcaact tggtattgca ggtatctctc ctgaacaaga accaagtcca
     5101 cgacaactaa cagaaattca ggaatttgtt aagacctata aggttaaaac gatttttaca
     5161 gaaagtaacg cttcttcaaa agtagctgaa actcttgtca aatcaacagg tgtgggtctt
     5221 aaaactctga atcctttaga gtcagaccca caaaatgaca agacctattt agaaaatctt
     5281 gaagaaaata tgagtattct agcagaagaa ttaaagtgag gaaagaatga aaattaataa
     5341 aaaatatcta gcaggttcag tggcagtcct tgccctaagt gtttgttcct atgagcttgg
     5401 acgttaccaa gctggtcagg ataagaaaga gtctaatcga gttgcttata tagatggtga
     5461 tcaggctggt caaaaggcag aaaacttgac accagatgaa gtcagtaaga gggaggggat
     5521 caacgccgaa caaattgtta tcaagattac ggatcaaggt tatgtgacct ctcatggaga
     5581 ccattatcat tactataatg gcaaggttcc ttatgatgcc atcatcagtg aagagctcct
     5641 catgaaagat ccgaattatc agttgaagga ttcagacatt gtcaatgaaa tcaagggtgg
     5701 ttatgtcatt aaggtaaacg gtaaatacta tgtttacctt aaggatgcag ctcatgcgga
     5761 taatattcgg acaaaagaag agattaaacg tcagaagcag gaacgcagtc ataatcataa
     5821 ctcaagagca gataatgctg ttgctgcagc cagagcccaa ggacgttata caacggatga
     5881 tgggtatatc ttcaatgcat ctgatatcat tgaggacacg ggtgatgctt atatcgttcc
     5941 tcacggcgac cattaccatt acattcctaa gaatgagtta tcagctagcg agttagctgc
     6001 tgcagaagcc tattggaatg ggaagcaggg atctcgtcct tcttcaagtt ctagttataa
     6061 tgcaaatcca gctcaaccaa gattgtcaga gaaccacaat ctgactgtca ctccaactta
     6121 tcatcaaaat caaggggaaa acatttcaag ccttttacgt gaattgtatg ctaaaccctt
     6181 atcagaacgc catgtggaat ctgatggcct t
//
LOCUS       DAVMMR010000084         5980 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMMR010000084 DAVMMR010000000
VERSION     DAVMMR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.084"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            481..1341
                     /locus_tag="VYS74_001887"
     CDS             481..1341
                     /locus_tag="VYS74_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3789877.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            1353..2246
                     /locus_tag="VYS74_001888"
     CDS             1353..2246
                     /locus_tag="VYS74_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789878.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            2259..3062
                     /locus_tag="VYS74_001889"
     CDS             2259..3062
                     /locus_tag="VYS74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3789879.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3095..3805)
                     /gene="treR"
                     /locus_tag="VYS74_001890"
     CDS             complement(3095..3805)
                     /gene="treR"
                     /locus_tag="VYS74_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV3789880.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3990..5957
                     /gene="treP"
                     /locus_tag="VYS74_001891"
     CDS             3990..5957
                     /gene="treP"
                     /locus_tag="VYS74_001891"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV3789881.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
BASE COUNT         1665 a         1113 c         1390 g         1812 t
ORIGIN      
        1 ctgaaagaat ttttgaaaaa ttttaaaaat tatgaaatgt tattgacagg tgatgaaaaa
       61 ggtggtagga caggaagcat caaaaaagaa agcagaaaaa gaaatgaata agagacaagc
      121 cgtaactatg aatcaaaact tttactttag ctactttaga tagtgtttgt agacatgtca
      181 ccatggatgc aaacagttca agcaatttgt ttgtatctat gtggctaaga tttttgattg
      241 tggtccatat agatgaatat ctaaatggac tagctaagtt gtaacttgtt aggttatttc
      301 aagtatccgt ttagatagta tctaggcggt tttttgtttt atcttttctg agaaggagtt
      361 tcattatgaa agttgttcga aaatcactag ccccctcttg gtagtggggc tcctcttgac
      421 ttctctgatc agtttgcatc agttgaaggc agataagaaa aagatgtctt tcgtatcggt
      481 atttcgcaat ttattaccca ccagtcctta tacgctacta gagaaggatt tgtggatgag
      541 ctggccaaac aaggctatgt tgagggggaa aatattgaga ttgatttgca aaatgcacag
      601 ggagaacaaa gaaatctaaa aacgatttcc cagcaactag cagaatccag cgatgtcgtt
      661 ctagccatcg caaggccttc tgctcagagc ttggccaata cgacgcaaac gacaccagtt
      721 atcttttcag ctgtaacaga cccagtcagt gccaagttgg ttgagtcaag agaacatcct
      781 gggggcaatg taactgggac aagcgatcag tcatcagatg ccatttcaac ccaaatcaat
      841 ttgataaaga aagtgttgcc aaaggctaaa acgattggaa tcctctatac tcagagcgag
      901 cccaattcgg ttgttcaaaa ggatgaagct aagcgacttc tgaaagaaaa aggctttacc
      961 gttgttgaaa aaacaatctt ggacagtaat aacgtcaagg cggcagcaga gagtttgatg
     1021 gcagaggtgg atatggtttt tgtcccaact gataatatca tttcgttaac catggaaaca
     1081 gttaagcagg tttctattaa acacaaggtt ccagtattcg gtggttcaac agaaatgatt
     1141 gctgttggtg gcttgtataa ctacggaacc aattatgaag aattggtacg tcagacagct
     1201 cgcatgctga ttcgtgtttt aaaaggtgag aaaccagaaa atatagcagt tgagttgcct
     1261 gaaaaactgg aattgcatac caatcaagaa atggcagatg cattgggaat tgatattagt
     1321 aagttagaag gcaaggaata aggaggatta agatgaattt tgtgttatct agtttatcag
     1381 aaggtttgtt gtggtcgatt gtggcgattg gggtctactt gactttccgt attttggata
     1441 ttgcggatat gactgctgag ggagcctttc ctctgggggc agcagtcgtt gtatcacaga
     1501 tacaggcagg ggcaaatcct tggcttgcga ctttacttgc tttgctggca ggtatggtag
     1561 caggtcttgt atcaggaatg ctccacacca agatgaaaat cccagctctc ttgacaggga
     1621 ttgtgacctt gacagggctc tattcaatca atattaaaat catgggcagt gtacccaatc
     1681 tttccttggg agattctgca accgtcttta aacagttggc gagcttgggt ctgacaaatg
     1741 aaggaactgt tttctcactt agtttggcct gttttttact tgtttgtttg gtcttgaccc
     1801 ttttgatgaa aacagagatt ggcttggtct tgcgttcgac tggggataat atcccgatga
     1861 gtgaggccaa tggagtcaat gtagacacca tgaagattgt tggttacatg atttcaaacg
     1921 gtttgattgc tctatgtggt tccttgtttg cccaaaatga tggattttca gatgtgactt
     1981 ctgggacagg aaccattgtt gttggtttga gctcggtgat tattgcggaa gtcttgatac
     2041 acgacttgac cattggaggc cgcttattat ccatcggaat cggtgctatt gtttaccgtt
     2101 tgattatttt aaatatctat gaaattccaa atctagatca aaacttggta cgtctcttta
     2161 atgcaatctt gctcgctttg gttttatttg caccagaatt gcaaaagaga ttaaagattc
     2221 gtggtctgaa attgagaaat gaataggagg agaaagttat gacaagtttg ttaacacttg
     2281 aaaatattca caagacattt gaagcaggga cggtcaatga gaaccatgtc ctcaaaggat
     2341 tagatttaga ggttgaagag ggagatttta tctctgtgat tggtggaaat ggagcaggaa
     2401 aatccacttt gatgaatatc ttggctggca atctatcggt ggacgaaggg gaccttctac
     2461 tggcagggaa atccattaaa aatctgagtg tcagaaagag agccaaggat attgcccgtg
     2521 tttttcaaga tcctaagatg ggaacagctt ctcgtttgac gattgaggag aatatggcta
     2581 ttgccttgag acgcgggcag aagagagggc ttggttgggg cgtgaaggag aaggatagaa
     2641 tccagttcca agaggccttg aaggagttga atattggtct tgaaaaccgc ttaaaagtag
     2701 atactcaata tctctcagga ggacaaagac aggctttgac tctagtcatg acagatctga
     2761 tgaaaccaaa acttttactt ttggatgaac atactgcggc acttgacccg aaaaccagtc
     2821 aaatggtgat ggacttgacg caaaagattg tggagcacca tcagtggacg actttgatga
     2881 ttacccatga tatgaatcat gcgattgagt acggcaatcg tctcattatg ctctatcaag
     2941 gcaagatagt ggtggatgtc aagggagaag agaaaaagca tctgacagtt gaagacctta
     3001 tgcatctctt ccagaaaaat agtggccaaa gtctggtcag tgatgaatta gttttgggat
     3061 aaagaaaaag gctgagatct ggtgtctcag tcttttattt cttgcgtttt gcaaaatcta
     3121 caaatctaaa tttctctaac ttatggcgac tttcggtaaa ctgaaattgg cgtccgtctt
     3181 ggagatagac ttttgattta atcgaaacga cataagggtc tttgccaatg tccatgagaa
     3241 tcttgtctcg gtcgcttgag tggtcaatgg tgatttcctt ctgagcataa tcaataagga
     3301 gtttgaggcc attttctata taagaataga tagactgctc agcaatttgg cgagtgagat
     3361 ttggaattag ttccatatcc agatagtccg tatccagaac ggataccaga tcatccacca
     3421 cacgctggcg gaccaccttc caaaccatcc gaaactctgg gaaaccggtt atcagtgagg
     3481 attttttatc aataataatc ttgtccagac tgaccacgtt ggttttagag cgcagtccaa
     3541 gttctttaac tagttcttgg tagctggtta ggttggatac agggaaattg acggtttctt
     3601 ctttgacgac ttgagaacct tgctctctta tctttttgat caatccttcc tcttggagga
     3661 gagacagggc ctttcggacg gtatcacgac tcacttgata ttgctccata aggtcgtgct
     3721 cgctaggaag gaaatctccg acagcgtaag tctcgttttg aatggtttct tggatttgct
     3781 taaataattg ttggtatttc ttcattttgc ctgctttaat aggtttttag tctagtttaa
     3841 tgaacacctg tatctatttt accatacaag tgctagaaaa tttacaaaaa aacacccttt
     3901 ttataccaaa caagttgcag acaagtttgg tatcgtttac aatatactta tcaaatcaaa
     3961 cttgctttga caagtataag gagaatcaaa tgggaaaatt tgaacaagaa gccaaagatc
     4021 tgcttcaggc aattggaggc aaagaaaatg tgactgccgt aactcactgt gcgacacgga
     4081 tgcgctttgt tttaggagat gataagaagg ctaatgttaa agctatcgag tcaattccag
     4141 ctgttaaagg aacttttaca aatgcaggtc aatttcaggt aatcattgga aatgacgtgc
     4201 ccatctttta taatgatttt acagccgttt caggtattga gggtgtttcc aaagaagcag
     4261 ccaagtctgc agctaagagt aatcaaaacg tggtccaacg tgttatgacc actctggcgg
     4321 agatttttac tccgattatt ccagccttga tagtcggagg attgatcctc ggtttccgta
     4381 atgtcttgga aggtgtccat tggtcgatgt tggatggcaa gaccatcaca gaatcctctc
     4441 agttttgggc aggtgtcaat cacttcctct ggttgcctgg tgaagctatc ttccagttct
     4501 taccagtagg gattacttgg tctgtttctc gtaagatggg aaccagccaa attttgggaa
     4561 ttgttctcgg aatctgtttg gtatcgcctc agttgctcaa tgcctatgcg gttgcttcaa
     4621 cgccagcagc tgatatcgcg gcaaactggg tttggaattt tggctatttt actgttaatc
     4681 gtatcggtta ccaagcccaa gttatcccag ccttgcttgc aggtttgagt ctgtcttatc
     4741 ttgaaatctt ctggcgcaag catatcccag aagtcatttc tatgattttt gtacctttct
     4801 tgtcattgat tccagccttg attttggctc atactgtttt gggaccaatc ggttggacaa
     4861 ttggacaagg actttcatca gttgtcttgg caggtttaac tggtccagtt aaagggctct
     4921 tcggtgcaat ttttggtgcc ctctacgctc catttgtcat cacaggtctg caccatatga
     4981 ccaatgccat tgatacacaa ttgattgcgg atgctggtgg cactgcccta tggccaatga
     5041 ttgctctttc taatattgct caaggctcag ccgtgtttgc ctattatttc atgcatcgcc
     5101 atgatgagcg tgaggctcag gtttcacttc ctgcaaccat ttcagcctat ctcggtgtta
     5161 cagaaccagc tctttttggg gttaacgtaa aatatattta tccatttgtt gctgggatga
     5221 ctggttcagc ccttgcaggc atgttatccg ttacttttaa tgtaactgcg gcttctattg
     5281 gtatcggtgg tttgccaggt attctctcta ttcaacctca atacatgctg ccatttgcag
     5341 gaactatgct agttgcgatt gttgttccaa tgctcttgac tttcttcttc cgcaaggctg
     5401 gtctctttac aaaaacagag ggcgatacga acttgcaggc agaattcgtt gctcaagaag
     5461 aagcagaatt tgtgaaccat gaaccagtag aacttacttc ggtagaaatt atcagcccac
     5521 taactggcca agtgaaagaa ttgagtcaag cgacggatcc tatttttgca tcaggtgtca
     5581 tggggcaagg tctggtcatt gaaccaagcc aaggtgagtt gacctctcca gttaatggga
     5641 cagtgacggt tcttttccct accaagcatg ccatcggcat tgtctctgac gagggagttg
     5701 aattgctcat ccacatcggt atggatacag taggtcttga tggcaaaggt tttgaaagtc
     5761 ttgtagtcca aggagatcac gttacagttg gtcagcaact gattcgtttt gatatggatg
     5821 tcattaaggc tgcaggtctg gtgacagaaa ctcctgttat catcaccaac caagatgctt
     5881 atacagcgac tattcccgga acttatccga caacgattca agctggagca tctctcatgg
     5941 tcgctacacg aatctaatac tcttcgaaaa tctcttcaaa
//
LOCUS       DAVMMR010000085         5404 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMMR010000085 DAVMMR010000000
VERSION     DAVMMR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.085"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            256..1635
                     /locus_tag="VYS74_001892"
     CDS             256..1635
                     /locus_tag="VYS74_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3789882.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            1649..2314
                     /locus_tag="VYS74_001893"
     CDS             1649..2314
                     /locus_tag="VYS74_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV3789883.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            2428..2523
                     /locus_tag="VYS74_001894"
                     /pseudo
     CDS             2428..2523
                     /locus_tag="VYS74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2669..3207
                     /locus_tag="VYS74_001895"
                     /pseudo
     CDS             2669..3207
                     /locus_tag="VYS74_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            3274..3684
                     /locus_tag="VYS74_001896"
     CDS             3274..3684
                     /locus_tag="VYS74_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV3789884.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            3800..>5404
                     /gene="pavB"
                     /locus_tag="VYS74_001897"
     CDS             3800..>5404
                     /gene="pavB"
                     /locus_tag="VYS74_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV3789885.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASLAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALINQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISK
                     ETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKAS
                     DKAPWSDNGDAKNPALSPLGENVKTKG"
BASE COUNT         1553 a         1047 c         1215 g         1589 t
ORIGIN      
        1 cctgtgacta gctttctaag cgatgccttg gttctcattg cctataatca aaaagagaaa
       61 ttttctcctg aaaagcatat agagtagctg gcgttaaaag ctcctgtctt gcttttttga
      121 cctatagtca catctatcaa gtattgttct tgcctaagct atcaataaaa aggtggcatt
      181 ttttaggctt ggtgttagtg aattttgcct tatcctatct aagtcatttc gagcttttta
      241 tggtacaatg gaaacatgtt attcaaatta tctaaggaaa aaatagagct aggcttatct
      301 cgtttatcgc cagcccgtcg tatttttttg agttttgcct tggtcatttt actaggctct
      361 cttcttttga gcttgccctt tgtccaagtt gaaagctcac gagcgactta ttttgatcat
      421 cttttcactg ctgtctctgc agtctgtgtg acaggtctct caacccttcc agtagctcac
      481 acctataata tatggggcca aataatctgt ttgctcttga ttcagatcgg tggtctaggg
      541 ctcatgacct ttattggggt tttctatatc cagagcaagc aaaagcttag tcttcgtagc
      601 cgtgcaacta ttcaggatag ttttagttat ggagaaactc gatctttgag aaagtttgtc
      661 tattctattt ttctcacgac ctttttggtt gagagcttgg gagctatttt gcttagtttt
      721 cgccttattc ctcaacttgg ctggggacgt ggtcttttta gttccatttt tctagcgatc
      781 tcagccttct gtaatgccgg ttttgataat ttagggagca ccagtttatt tgcttttcag
      841 accgatttac tggtcaatct ggtgattgca ggcttgatta ttacaggcgg ccttggtttt
      901 atggtctggt ttgatttggc tggtcatgta ggaagaaaga aaaaaggacg tctgcacttt
      961 catacgaagc ttgtactatt gttgactata ggtttgttgt tatttggaac ggcaactact
     1021 ctctttcttg agtggaacaa tgctggaacg attggcaatc tccctgttgc cgataaggtt
     1081 ttagttagct tttttcaaac agtgacgatg cgaacagctg gcttttctac gatagattat
     1141 actcaggctc atcctgtgac tcttttgatt tatatcttac agatgtttct aggtggggca
     1201 cctggaggaa cagctggggg actcaagatt acgacatttt ttgtcctctt ggtctttgca
     1261 cgaagtgagc ttctaggctt gcctcatgcc aatgttgcga gacgaacgat tgcgccgcga
     1321 acggttcaaa aatcctttag tgtctttatt atctttttga tgagcttctt gataggattg
     1381 attctgctag ggataacagc caaaggcaat cctcccttta tccacctcat atttgaaacc
     1441 atttcagctc ttagtacagt tggtgtaacg gcaaatctga ctcctgacct tgggaaattg
     1501 gctctcagtg ttatcatgcc acttatgttt atgggacgaa ttggtccctt gaccttgttt
     1561 gttagcttgg cagattacca tccagaaaag aaagatatga ttcactatat gaaagcagat
     1621 attagtattg gttaagaaag gaaagagcat gtcagatcgt acgattggaa ttttgggctt
     1681 gggaattttt gggagcagtg tcctagctgc cctagccaag caggatatga atattatcgc
     1741 tattgatgac cacgcagagc gcatcaatca gtttgagcca gttttggcgc gtggagtgat
     1801 tggtgacatc acagatgaag aattattgag atcagcaggg attgatacct gcgataccgt
     1861 tgtagtcgcg acaggtgaaa atctggagtc gagtgtgctt gcggttatgc actgtaagag
     1921 tttgggggta ccgactgtta ttgctaaggt caaaagtcag accgctaaga aagtgctaga
     1981 aaagattgga gctgactcgg ttatctcgcc agagtatgaa atgggacagt ctctagcaca
     2041 gaccattctt ttccataata gtgttgatgt ctttcagttg gataaaaatg tgtctatcgt
     2101 ggagatgaaa attcctcagt cttgggcagg tcaaagtctg agtaaattag acctccgtgg
     2161 caaatacaat ctgaatattt tgggtttccg agagcaggaa aattccccat tggatgttga
     2221 atttggacca gatgacctct tgaaagcaga tacctatatt ttggcagtca tcaacaacca
     2281 gtatttggat accctagtag cattgaattc gtaaagaggg atgacccctc ttttttgatg
     2341 cccaagatgg caaatagaga cagaagcccc ttgtcttcta gtaaaagttc ttcaaaggct
     2401 ggactttatg gtaaaataga aggaagtgac aagagagagt aatactcaat gaaaatcaaa
     2461 gatcaaacta ggaaactagc tgcgggctgc tcaaaacact gttttgaggt tgtggataga
     2521 actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
     2581 aacatctata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtataaga
     2641 aaaaatcagt tccctaaagg agcagattat gaagttattg tctatcgcta tttctagcta
     2701 taatgcagca gcctatcttc attactgtgt ggagtcgcta gtgattggtg gtgagcaagt
     2761 tgggattttg attatcaatg acgggtctca ggaccagact caggaaatcg ctgagtgttt
     2821 agctagcaag tatcctaata tcgttagagc catctatcag gaaaataaag gacatggcgg
     2881 tgtggtcaat cgcggcttgg cagaggcttc tgggcgctat tttaaagtag ttgacagtga
     2941 tgactggtgg atcctcgtgc ctacttgaaa attcttgaaa ccttgcagga acttgagagc
     3001 aagggtcaag agatggatgt ctttgtgacc aattttgtct atgaaaagga aggtcagtct
     3061 cgtaagaaga gtatgagtta cgagtcagtc ttgcctgttc ggcagatttt tggctgggac
     3121 caggtcggaa atttctccaa aggccagtat atcatgatgc actcgctgat ttatcggaca
     3181 gctttgttgc gtgctagcca gttctaactg cctgaacata ctttttatgt cgataatctc
     3241 tttgtcttta cgccccttca gcaggtcaag accatgtact atctgcctgt cgatttctat
     3301 cgttatttga ttgggcgtga ggaccagtct gtcaatgagc aagtgatgat taagtgcatt
     3361 gaccagcaac tcaaggtcaa tcgactcttg gtagaccagc ttgatttgtc ccaagtgagt
     3421 catcccaaaa tgcgagaata tctgctgaat catattgaaa tcacgacggt gatttccagt
     3481 accctgctca accgatctga aacagcggag catctggcaa aaaaacgcca attgtggacc
     3541 tatattcagc agaaaaatcc agaagtcttt caggctattc gtaagaccat gttgagccgt
     3601 ttgaccaaac attctgtctt gccagatcgc aaactgtcca atgtcgtcta tcaaatcacc
     3661 aaatctgttt atggatttaa ttaatataag tgttttataa gagggattta agaaaaattt
     3721 taactttttc ttagtccttt ttaatttcag gagattatac tagagtcatc aaataaagaa
     3781 agactctaag gagaatccta tgaaattcaa tccaaatcaa agatatactc gttggtctat
     3841 tcgccgtctc agtgtcggtg ttgcctcagt tgttgtggct agtggcttct ttgtcctagt
     3901 tggtcagcca agttctgtac gtgccgatgt ggtcaatccg accccaggtc aagtcttacc
     3961 tgaagagaca tcgggaacga aagagggtga cttatcagaa aaaccaggag acaccgttct
     4021 cactcaagcg aaacctgagg gcgttactgg aaatacgaat tcacttccga cacctacaga
     4081 aagaactgaa gtgagcgagg aaacaaactc ttctagtctg gatacacttt ttgaaaaaga
     4141 tgaagaagct caaaaaaatc cagagctaac agatgtctta aaagaaactg tagatacagc
     4201 tgatgtggat gggacacaag caagtctagc agaaactact cctgaacaag taaaaggtgg
     4261 agtgaaagaa aatacaaaag acagcatcga tgttcctgct gcttatcttg aaaaagctga
     4321 agggaaaggt cctttcactg ccggtgtaaa ccaagtaatt ccttatgaac tattcgctgg
     4381 tgatggtatg ttaactcgtc tattactaaa agcttcggat aatgctcctt ggtctgacaa
     4441 tggtactgct aaaaatcctg ctttacctcc tcttgaagga ttaacaaaag ggaaatactt
     4501 ctatgaagta gacttaaatg gcaatactgt tggtaaacaa ggtcaagctt taattgatca
     4561 acttcgcgct aatggtactc aaacttataa agctactgtt aaagtttacg gaaataaaga
     4621 cggtaaagct gacttgacta atctagttgc tactaaaaat gtagacatca acatcaatgg
     4681 attagttgct aaagaaactg tcgaaaaagc tgttaaagat aacgttaaag acagtatcga
     4741 tgttccagca gcctacctag aaaaagccaa gggtgaaggt ccattcacag caggtgtcaa
     4801 ccatgtgatt ccatacgaac tcttcgcagg tgatggtatg ttgactcgtc tcttgctcaa
     4861 ggcatctgac aaggcaccgt ggtcagataa cggtgacgct aaaaacccag ccctatctcc
     4921 actaggtgaa aacgtgaaga ccaaaggtca atacttctat caagtagcct tggacggaaa
     4981 tgtagctggc aaagaaaaac aagcgctcat taaccagttc cgagcaaatg gtactcaaac
     5041 ttacagcgct acagtcaatg tctatggtaa caaagacggt aaaccagatt tggacaacat
     5101 cgtagcaact aaaaaagtca ctattaacat aaacggttta atttctaaag aaacagttca
     5161 aaaagccgtt gcagacaacg ttaaggacag tatcgatgtt ccagcagcct acctagaaaa
     5221 agccaagggt gaaggtccat tcacagcagg tgtcaaccat gtgattccat acgaactctt
     5281 cgcaggtgat ggtatgttga ctcgtctctt gctcaaggca tctgacaagg caccatggtc
     5341 agataacggt gacgctaaaa acccagccct atctccacta ggtgaaaacg tgaagaccaa
     5401 aggt
//
LOCUS       DAVMMR010000086         5230 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMMR010000086 DAVMMR010000000
VERSION     DAVMMR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.086"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            55..501
                     /gene="argR"
                     /locus_tag="VYS74_001898"
     CDS             55..501
                     /gene="argR"
                     /locus_tag="VYS74_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3789886.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            552..3086
                     /gene="mutS"
                     /locus_tag="VYS74_001899"
     CDS             552..3086
                     /gene="mutS"
                     /locus_tag="VYS74_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV3789887.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3233..4927
                     /gene="patA"
                     /locus_tag="VYS74_001900"
     CDS             3233..4927
                     /gene="patA"
                     /locus_tag="VYS74_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV3789888.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1438 a          999 c         1277 g         1516 t
ORIGIN      
        1 tttggtataa tatggttata aatatagtta taaatatgca cgcaagagga ttttatgaga
       61 aaaagagatc gtcatcagtt aataaaaaaa atgattactg aggagaaatt aagtacacaa
      121 aaagaaattc aagatcggtt ggaggcgcac aatgtttgtg tgacgcagac aaccttgtct
      181 cgtgatttgc gcgaaatcgg cttgaccaag gtcaagaaaa atgatatggt gtattatgta
      241 ctagtaaatg agacagaaaa gattgatttg gtggaatttt tgtctcatca tttagaaggt
      301 gttgcaagag cagagtttac cttggtgctt cataccaaat tgggagaagc ctctgttttg
      361 gcaaatattg tagatgtaaa caaggatgaa tggattttag gaacagttgc tggtgccaat
      421 accttattgg ttatttgtcg agatcagcac gttgccaaac tcatggaaga tcgtttgcta
      481 gatttgatga aagataagta aggtcttggg agttgctctc aagacttatt tttgaaaagg
      541 agagacagaa aatggcgata gaaaagctat cacccggcat gcaacagtat gtggatatta
      601 aaaagcaata tccagatgct tttttgctct ttcggatggg tgatttttat gaattatttt
      661 atgaggatgc ggtcaatgct gcgcagattc tggaaatttc cttaacgagt cgcaacaaga
      721 atgccgacaa tccgatccct atggcgggtg ttccctatca ttctgcccaa cagtatatcg
      781 atgtcttgat tgagcagggt tataaggtgg ctatcgcaga gcagatggaa gatcctaaac
      841 aagcagttgg ggttgttaaa cgagaggttg ttcaggtcat tacgccaggg acagtggtcg
      901 atagcagtaa gccggacagt cagaataatt ttttggtttc catagaccgc gaaggcaatc
      961 aatttggcct agcttatatg gatttggtga cgggtgactt ttatgtgaca ggtcttttgg
     1021 atttcacgct ggtttgtggg gaaatccgta acctcaaggc tcgagaagtg gtgttgggtt
     1081 atgacttgtc tgaggaagaa gaacaaatcc tcagccgcca gatgaatctg gtactctctt
     1141 atgaaaaaga aagctttgaa gaccttcatt tattggattt gcgattggca acggtggagc
     1201 aaacggcatc tagtaagctg ctccagtatg ttcatcggac tcagatgagg gaattgaacc
     1261 acctcaaacc tgttatccgc tacgaaatta aggatttctt gcagatggat tatgcgacca
     1321 aggctagtct ggatttggtt gagaatgctc gctcaggtaa gaaacaaggc agtcttttct
     1381 ggcttttgga tgaaaccaaa acggctatgg ggatgcgtct cttgcgttct tggattcatc
     1441 gccccttgat tgataaggaa cgaatcgtcc aacgtcaaga agtagtgcag gtctttctcg
     1501 accatttctt tgagcgtagt gacttgacag acagtctcaa gggtgtttat gacattgagc
     1561 gcttggctag tcgtgtttct tttggtaaaa ccaatccaaa ggatctcttg cagttggcga
     1621 ctaccttgtc tagtgtgcca cggattcgtg cgattttaga agggatggag caacctactc
     1681 tagcctatct catcgcacaa ctggatgcaa tccctgagtt ggagagtttg attagcgcag
     1741 cgattgctcc tgaagctcct catgtgatta cagatggggg aattatccgg actggatttg
     1801 atgagacttt agacaagtat cgttgcgttc tcagagaagg gactagctgg attgctgaga
     1861 ttgaggctaa ggagcgagaa aactctggta tcagcacgct caagattgac tacaataaaa
     1921 aggatggcta ctattttcat gtgaccaatt cgcaactggg aaatgtgcca gcccactttt
     1981 tccgcaaggc gacgctgaaa aactcagaac gctttggaac cgaagaatta gcccgtatcg
     2041 agggagatat gcttgaggcg cgtgagaagt cagccaacct cgaatacgaa atatttatgc
     2101 gcattcgtga agaggtcggc aagtacatcc agcgtttaca agctctagcc caaggaattg
     2161 cgacggttga tgtcttacag agtctggcgg ttgtggctga aacccagcat ttgattcgac
     2221 ctgagtttgg tgacgattca caaattgata tccggaaagg gcgccatgct gtcgttgaaa
     2281 aggttatggg ggctcagacc tatattccaa atacgattca gatggcagaa gataccagta
     2341 ttcaactggt tacagggcca aacatgagtg ggaagtctac ctatatgcgt cagttagcca
     2401 tgacggcggt tatggcccag ctgggttcct atgttcctgc tgaaagcgcc catttaccga
     2461 tttttgatgc gatttttacc cgtatcggag cagcagatga cttggtttcg ggtcagtcaa
     2521 cctttatggt ggagatgatg gaggccaata atgccatttc gcatgcgacc aagaactctc
     2581 tcattctctt tgatgaattg ggacgtggaa ctgcaactta tgacgggatg gctcttgctc
     2641 agtccatcat cgaatatatc catgagcaca tcggagctaa gaccctcttt gcgacccact
     2701 accatgagtt gactagtctg gagtctagtt tacaacactt ggtcaatgtc cacgtggcaa
     2761 ctttggagca ggatgggcag gtcaccttcc ttcacaagat tgaaccggga ccagctgata
     2821 aatcctacgg tatccatgtt gccaagattg ctggcttgcc agcagacctt ttagcaaggg
     2881 cggataagat tttgactcag ctagagaatc aaggaacaga gagtcctcct cccatgagac
     2941 aaactagtgc tgtcactgaa cagatttcac tctttgatag ggcagaagag catcctatcc
     3001 tagcagaatt agctaaactg gatgtgtata atatgacacc tatgcaggtt atgaatgtct
     3061 tagtagagtt aaaacagaaa ctataaaacc aagactcact agttaatcta gctgtatcaa
     3121 ggagacttct ttgacaattc tccacttttt tgctagaata acatcacaca aacagaatga
     3181 aaaggagctg acgcattgtc gctccctttt gtctattttt taaggagaaa gtatgctgat
     3241 tcagaaaata aaaacctaca agtggcaggc cctggcttcg ctcctgatga caggcttgat
     3301 ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg ccctccttgc
     3361 tgggaaatat gaagctattt atagtatcgg ggcttggttg attggtgtgg ccgtagtcgg
     3421 tctagttgct ggtggactca atgttgtcct cgcagcctat attgcccaag gagtttcatc
     3481 cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg atattgaaca
     3541 atttaatgcg ggaaatctag tcgttcgaat gacaaatgat atcaaccaga ttcagaacgt
     3601 tgtcatgatg accttccaga ttcttttcag acttcccctc ttgttcatcg gttcgtttat
     3661 cctagcggtt caaaccttac cttctctgtg gtgggtgatt gttctcatgg tagtcttgat
     3721 ttttggtttg actgctgtca tgatgggaat gatggggcct cgttttgcca agtttcaaac
     3781 ccttcttgag cgcatcaatg ccattgccaa ggaaaattta cgtggcgttc gtgtggtcaa
     3841 gtcctttgtc caagaaaaag agcaatttgc taagtttaca gaggtctcag acgagcttct
     3901 tggtcaaaac ctttacattg gttatgcctt ttcagtagtg gaacccttta tgatgttggt
     3961 tggttacggg gcggtcttcc tctctatttg gctggtcgcg ggaatggttc agtcggatcc
     4021 gtctgttgtt ggttccatcg cttcttttgt taattaccta agccagatta tctttaccat
     4081 tgttatggtt ggatttttgg gaaattctgt cagccgtgcc atgatttcca tgcgtcgtat
     4141 tcgagaaatt cttgacgcag agccagctat gaccttcaag gatatcccag atgaagagtt
     4201 ggttggaagc cttagctttg aaaatgtgac ctttacctat ccaatggaca aggaaccgat
     4261 gctgaaagat gtgagcttta ctattgaacc tggtcaaatg gttggtgtag ttggagcgac
     4321 tggtgcagga aagtcaacct tggctcaatt gattccacgt ctctttgatc cacaggaagg
     4381 ggctatcaaa atcggaggta aggatattcg agaagtgagt gaaggaaccc tgcgtaaaac
     4441 agtttccatc gttctccaac gtgccattct ttttagtgga acgattgcag ataacttgag
     4501 acaggggaag ggaaatgcta ctctatttga aatggagcgc gcagccaata ttgcccaggc
     4561 tagtgaattc attcatcgta tggagaaaac ctttgaaagt ccagttgaag aacggggaac
     4621 caatttctct ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg tcagcaatcc
     4681 acgtattctg atttttgatg attcgacctc agccttggat gccaaatcag agcgcttggt
     4741 gcaagaagct ttgaataagg acttgaaggg gacgacaacc attattattg ctcaaaaaat
     4801 tagctcggtt gtccatgcag acaagatctt ggttctaaat caaggacgat tgattggtca
     4861 aggcacgcat gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct atgaaacaca
     4921 gaaatgaaag acaaactata agaaaagtca atagttttat ctaaactatt tcttatttca
     4981 atttgatgat ttggcgatga ttttagagca cggcaaaaag ctattgaaaa agtccatttt
     5041 ttcaaaggta atcctgtgtt aatttcagaa attacatcac tttttgttcg tcaaatggca
     5101 gctctttttt aggatataaa acagggttcg gataagtttt tttgcaaggt ggatgatggc
     5161 tacattgtaa tgttttcctt gttctaactt agtcttaaga taggccttaa aagcaggtga
     5221 aaagcgaggg
//
LOCUS       DAVMMR010000087         5206 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMMR010000087 DAVMMR010000000
VERSION     DAVMMR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.087"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..1166
                     /gene="lytB"
                     /locus_tag="VYS74_001901"
     CDS             <1..1166
                     /gene="lytB"
                     /locus_tag="VYS74_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV3789889.1"
                     /translation="TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1227..2882)
                     /gene="pavA"
                     /locus_tag="VYS74_001902"
     CDS             complement(1227..2882)
                     /gene="pavA"
                     /locus_tag="VYS74_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV3789890.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2987..3484
                     /gene="ybeY"
                     /locus_tag="VYS74_001903"
     CDS             2987..3484
                     /gene="ybeY"
                     /locus_tag="VYS74_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV3789891.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3465..3860
                     /locus_tag="VYS74_001904"
     CDS             3465..3860
                     /locus_tag="VYS74_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV3789892.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3877..4776
                     /gene="era"
                     /locus_tag="VYS74_001905"
     CDS             3877..4776
                     /gene="era"
                     /locus_tag="VYS74_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV3789893.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4825..>5206
                     /locus_tag="VYS74_001906"
     CDS             4825..>5206
                     /locus_tag="VYS74_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789894.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYF"
BASE COUNT         1490 a          934 c         1210 g         1572 t
ORIGIN      
        1 tgacagccaa tgaatggatt tgggataagg aatcttggtt ttatctcaaa tctgatggga
       61 aaatagctga aaaagaatgg gtctacgatt ctcatagtca agcttggtac tacttcaaat
      121 ccggtggtta catgacagcc aatgaatgga tttgggataa ggaatcttgg ttttacctca
      181 agtctgatgg caaaatggct gaaaaagaat gggtctacga ttctcatagt caagcctggt
      241 actacttcaa atctggtggc tacatggcga aaaatgagac agtagatggt tatcagcttg
      301 gaagcgatgg taaatggctt ggaggaaaag ctacaaataa aaatgctgct tactatcaag
      361 tagtgcctgt cacagccaat gtttatgatt cagatggtga aaagctttcc tatatatcgc
      421 aaggtagtgt cgtatggcta gataaggata gaaaaagtga tgacaagcgc ttggctatta
      481 ctatttctgg tttgtcaggc tatatgaaaa cagaagattt acaagcgcta gatgctagta
      541 aggactttat cccttattat gagagtgatg gccaccgttt ttatcactat gtggctcaga
      601 atgctagtat cccagtagct tctcatcttt ctgatatgga agtaggcaag aaatattatt
      661 cggcagatgg cctgcatttt gatggtttta agcttgagaa tcccttcctt ttcaaagatt
      721 taacagaggc tacaaactac agtgctgaag aattggataa ggtatttagt ttgctaaaca
      781 ttaataatag ccttttggag aacaagggcg ctacttttaa ggaagccgaa gaacattatc
      841 atatcaatgc cctttatctc cttgcccata gtgccctaga aagtaactgg ggaagaagta
      901 aaattgccaa agataagaat aatttctttg gcattacagc ctatgatacg accccttacc
      961 tttctgccaa gacatttgat gatgtggata agggaatttt aggtgcaacc aagtggatta
     1021 aggaaaatta tatcgatagg ggaagaactt tccttggaaa caaggcttct ggtatgaatg
     1081 tggaatatgc ttcagaccct tattggggcg aaaaaattgc tagtgtgatg atgaaaatca
     1141 atgagaagct aggtggcaaa gattagtact ataagtgaat atgatttgag tgaatagtaa
     1201 gttaaaaatc ctgatttcaa gtaaaatcag gattttttca tggatgcaat ttttttggag
     1261 tctggtgtga cgcggagggt cttttgtcct gtgtaagtga caaagccggg ttttccacca
     1321 gttggtttat tgagtttttt gacttcaatc atatctacct gcaccagatt cgacaggcgc
     1381 ccttgagaga agtaggcagc taactctgct gcgtctgtct tgactgcatc agatgggtca
     1441 agatttcctg agatgacaac atggcttcca ggaatgtcct tagcatggaa ccaaagttcc
     1501 tccttgcggg ccattttaaa ggtcaattcc tcattttgaa gattgtttcg tccgacatag
     1561 atgatggttt tgccatcgct tgctagatat tgttctagtt ttttgcgttt ctggattttc
     1621 tcccgttgtc ttctgcggat aaaacctgtt tgaatcaatt cttcacggat ttcagcgatt
     1681 tcttccagtc cagcttggtt gaggacggtt tctacacttt ccagatagag aatagtggct
     1741 ttggtttctt caatcaaatc agtcaagtat ttgacagctt ctttgagttt ctgataccgt
     1801 ttaaaatagc gttgggcatt ctggttggga gtcagagcct tatcaagcgc aatcatgata
     1861 ggttggttgg tatagtagtt gtctaggata acctggtctt ggtcgttagg cacttggtgt
     1921 aggaaggttg tcagcaattc tcctttttga cgaaattctt cagcgttgtc tgtcgccagt
     1981 aactcttttt cctgtttttt gagtttgtgt cggtttttct gaagttcatt ttcaacacga
     2041 cgaatcagtt cactggcctg ctgtttgacg cggtcgcgct cagccttatc cttatagtag
     2101 gtgtccaaca aatcagaaag atttgcaaaa ggctctccca cctgatttgc aaaaggaact
     2161 ggactgaagg aagtctcagt caagcatggc ttggtttctt gattgaaaaa atttcggaaa
     2221 gcggaaagtt tttcactaac cagtatcctt tccaattcat ttgcagtatc gcgtcccaga
     2281 ccttgaaaga ggctttgaag attttttgct gttagttctt gggtttgcag gatttcaaag
     2341 agcttttcat ctttgatagt aaaaggattg agagattctg tacttggcgg agcgatatag
     2401 gtcgatcctg gaagtaaggt gcggtagcta ttttgtgaaa aaccgacgtg tttgataact
     2461 tcgaggattt tatgactgct tttatcgacc agtagaatat tactgtgttt ccccataatt
     2521 tcaataatca aggtagcctg gatatggtct ccaatctcgt ttttattgga aactgtaatt
     2581 tccacaatac ggtcattttc cacttgctca atcgactcaa tcagggcccc ctgcaaatac
     2641 tttctcaaaa ccatgataaa ggtagaaggt tgagctggat tttcaaaagt cgtttgggtc
     2701 agctgaatgc gtccaaaaac tggatgggca gaaaggagca ggcgatggct ttggcgattg
     2761 ctgcggattt gcaagaccaa ctcttgttca aaaggctgat tgattttctg gatgcgacca
     2821 ttcactaatt cgcttcgcaa ttcctcaact atgtggtgta aaaaaaatcc gtcaaatgac
     2881 atcgttctct ccttgtgatt gtattccata gtattatatc aaaaaggtag aataaaatca
     2941 tggaaatgtg gtataataaa gccaagtaaa gagaaacgag aagcacatgt atattgaaat
     3001 ggtagatgaa actggtcaag tttcaaaaga aatgttgcaa caaacccaag aaattttgga
     3061 atttgcagcc caaaaattag gaaaagaaga caaggagatg gcagtcactt ttgtgaccaa
     3121 tgagcgtagt catgaactta atctggagta ccgtgacacc gaccgtccga cagatgtcat
     3181 cagccttgag tataaaccag aattggaaat tgcctttgac gaagaggatt tgcttgaaaa
     3241 tccagaattg gcagagatga tgtctgagtt tgatgcctat attggggaat tgttcatctc
     3301 tatcgataag gctcatgagc aggccgaaga atatggtcac agctttgagc gtgagatggg
     3361 cttcttggca gtacacggct ttttacatat taacggctat gatcactata ctccggaaga
     3421 agaagcggag atgttcggtt tacaagaaga aattttgaca gcctatggac tcacaagaca
     3481 ataaacgaaa atggaaaaat cgtgacttga tatccagttt agaatttgcc atcacaggga
     3541 tttttactgc tatcaaggaa gaacgaaata tgcgaaaaca tgcagtgacg gctctagtgg
     3601 tcatccttgc aggttttgtt tttcaggtgt cacgaatcga atggctcttt cttctattga
     3661 gtattttctt ggtagtagcc tttgagatta tcaactctgc tattgaaaat gtggtggatt
     3721 tggccagtca ctatcacttt tccatgctgg ctaaaaatgc caaggatatg gcggccggcg
     3781 cggtattagt ggtttctctt tttgcagcct taacaggcgc attgattttt ctcccacgaa
     3841 tctgggattt attattttaa acagtaagag gaaattatga cttttaaatc aggctttgta
     3901 gccattttag gacgtcccaa tgttgggaag tcaacctttt taaatcacgt tatggggcaa
     3961 aagattgcca tcatgagtga caaggcgcag acaacgcgca ataaaatcat gggaatttac
     4021 acgactgata aggagcaaat tgtctttatc gacacaccag ggattcacaa acctaaaaca
     4081 gctctcggag atttcatggt tgagtctgcc tacagtaccc ttcgcgaagt ggataccgtt
     4141 cttttcatgg tgcctgctga tgaagcgcgt ggtaaggggg acgatatgat tatcgagcgt
     4201 ctcaaggctg ccaaggttcc tgtgattttg gtggtgaata aaatcgataa ggtccatcca
     4261 gaccagctct tgtctcagat tgatgacttc cgtaatcaaa tggactttaa ggaaattgtt
     4321 ccaatctcag cccttcaggg aaataacgtg tctcgtctag tggatatttt gagtgaaaat
     4381 ctggatgaag gtttccaata tttcccgtct gatcaaatca cagatcatcc agaacgtttc
     4441 ttagtttcag aaatggttcg cgagaaagtc ttgcacctaa ctcgtgaaga gattccgcat
     4501 tctgtagcag tagttgttga ctctatgaaa cgagacgaag agacagacaa ggttcacatc
     4561 cgtgcaacca tcatggtcga gcgcgatagc caaaaaggga ttatcatcgg taaaggtggc
     4621 gctatgctta agaaaatcgg tagtatggcc cgtcgtgata tcgaactcat gctaggagac
     4681 aaggtcttcc tagaaacctg ggtcaaggtc aagaaaaact ggcgcgataa aaagctagat
     4741 ttggctgact ttggctataa tgaaagagaa tactaagtag aggtaggctc atgcctgctt
     4801 cttgttttta cagaaggagg acttatgcct gaattacctg aggttgaaac cgtttgtcgt
     4861 ggcttagaaa aattgattat aggaaagaag atttcgagta tagaaattcg ctaccccaag
     4921 atgattaaga cggatttgga agagtttcaa agggaattgc ctagtcagat tatcgagtca
     4981 atgggacgtc gtggaaaata tttgcttttt tatctgacag acaaggtctt gatttcccat
     5041 ttgcggatgg agggcaagta tttttactat ccagaccaag gacctgaacg caagcatgcc
     5101 catgttttct ttcattttga agatggtggc acgcttgttt atgaggatgt tcgcaagttt
     5161 ggaaccatgg aactcttggt gcctgacctt ttagacgcct acttta
//
LOCUS       DAVMMR010000088         5180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMMR010000088 DAVMMR010000000
VERSION     DAVMMR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.088"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            1..638
                     /locus_tag="VYS74_001907"
                     /pseudo
     CDS             1..638
                     /locus_tag="VYS74_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            829..3120
                     /gene="pcrA"
                     /locus_tag="VYS74_001908"
     CDS             829..3120
                     /gene="pcrA"
                     /locus_tag="VYS74_001908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV3789895.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3163..3843
                     /gene="radC"
                     /locus_tag="VYS74_001909"
     CDS             3163..3843
                     /gene="radC"
                     /locus_tag="VYS74_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV3789896.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3840..4529)
                     /locus_tag="VYS74_001910"
     CDS             complement(3840..4529)
                     /locus_tag="VYS74_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3789897.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4547..>5180)
                     /locus_tag="VYS74_001911"
     CDS             complement(4547..>5180)
                     /locus_tag="VYS74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV3789898.1"
                     /translation="KQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIGIDS
                     ATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLHYRF
                     HERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSVKSQ
                     EVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
BASE COUNT         1529 a         1026 c         1136 g         1489 t
ORIGIN      
        1 gcctagaaga ccttcttatg gccactcttc actatgtgcg agaatatcga acttatgaag
       61 aaattgcggc tgattttggt atccacgaaa gcaacttact ccgtcggagc caatgggttg
      121 aagtaactct tgttcaaagg tggtgttacg atttcaagaa ctcctctcag ctctgaggac
      181 acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaga attagcgaat
      241 cattctggta aaaagaaatt tcacgctatg aaggctcaag cgattgtcac aagtcaaggg
      301 agaattgttt ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatattggac
      361 aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata tatcctcaag
      421 cacaaactcc acgtaaatcc agcaaactca agccactaat agctgaagat aaagcttata
      481 accatgcgct atctaaggag agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt
      541 ttaaaatgtt ttcaacaacc tatcgaagtc atcgtaaacg cttcggatta cgaatgaatt
      601 tgagtgctgg tattatcaat catgaactag gattctagtt ttgcaggaag tctattgagg
      661 tattgagcta gtttatgaaa aaattgggtg aaaagtcgag tgttttagaa acccacagtg
      721 tagtattcta gtttcaatcc actatatttt gctactcccc gtaaagtttc tattttccct
      781 gatttctgat ataatagaaa tattgacttc aagagtaagg aagagaagat gaacgcatta
      841 ttaaatggaa tgaatgaccg tcaggctgag gcggtgcaaa cgacagaagg tcccttgcta
      901 atcatggcag gggctggttc tggaaagact cgtgttttaa cccaccgtat cgcttatttg
      961 attgatgaaa agctggtcaa tccttggaat atcttggcca ttacctttac caacaaggct
     1021 gcgcgtgaga tgaaagagcg tgcttatagc ctcaatccag ctactcagga ctgtctgatt
     1081 gcgaccttcc actccatgtg tgtgcgtatt ttgcgtcgcg atgcggacca tattggctac
     1141 aatcgtaatt ttacaattgt ggatcctggt gaacagcgaa cgctcatgaa acgtattctc
     1201 aaacagttga acttggatcc taaaaaatgg aatgaacgaa ctattttggg gaccatttcc
     1261 aatgctaaga ataatttgat tgatgatgtt gcttatgctg cccaagctgg cgatatgtat
     1321 acgcaaattg tggcccagtg ttatacagcc tatcaaaaag aacttcgtca gtctgaatcc
     1381 gttgactttg atgatttgat tatgctgacc ttgcgtctct ttgatcaaaa tcctgatgtt
     1441 ttgacctact accagcaaaa attccaatac atccacgttg atgagtacca agataccaac
     1501 cacgctcagt accaattggt caaactcttg gcttcccgtt ttaaaaatat ctgtgtggtt
     1561 ggggatgcgg accagtctat ctacggttgg cgtggtgctg atatgcagaa tatcttggac
     1621 tttgaaaagg attaccccaa agccaaggtt gtcttgctgg aggaaaatta ccgctcaacc
     1681 aaaaccattc tccaagcggc caacgaggtt attaaaaata ataaaaatcg ccgtcctaaa
     1741 aatctctgga ctcaaaacgc tgatggggag caaatcgttt actatcgtgc cgatgatgag
     1801 ctggatgagg ctgtatttgt agccagaacc atcgatgaac ttagtcgcag tcaaaacttc
     1861 cttcataagg attttgcagt tctctatcgg actaatgccc agtcccgtac aattgaggaa
     1921 gccctgctca agtctaacat tccttatacc atggttggcg gaaccaaatt ctacagccgt
     1981 aaggaaattc gcgatattat tgcttatctc aaccttattg ctaatttgag tgacaatatt
     2041 agttttgagc gtattatcaa cgagcctaaa cgtggaattg gtctaggtac agttgagaaa
     2101 atccgtgatt ttgcaaattt gcaaaatatg tctatgctgg atgcttctgc taatattatg
     2161 ttgtctggta tcaagggtaa ggcagcccaa tctatctggg attttgccaa tatgatgctt
     2221 gatttgcggg agcagctaga ccacttaagc attacagagt tggttgagtc cgtcctagaa
     2281 aaaacaggtt atgtcgatat tcttaacgcc caagcgactc tagaaagcaa ggcacgggtt
     2341 gaaaatatcg aagagtttct ttctgttacg aagaactttg atgacaccac ggatgtgaca
     2401 gaagaggaaa ctggtctgga caaactgagt cgtttcttaa atgacttggc tttgattgcc
     2461 gacacagatt caggtagtca ggagacatca gaagtgacct tgatgaccct gcatgctgcc
     2521 aaaggtctcg aatttccagt tgtctttttg attgggatgg aagaaaatgt ctttccactt
     2581 agtcgtgcga ctgaagatcc agatgaatta gaagaagagc gccgtctagc ctatgtaggt
     2641 atcacgcgtg cagagaaaat tctctatctg accaatgcca actcacgctt gctttttggt
     2701 cgtaccaatt ataaccgtcc gactcgtttt attaacgaaa tcagttcaga cttgcttgag
     2761 tatcaaggtc tggctcgtcc tgcaaataca agctttaagg catcatatag cagtggtagt
     2821 atttcctttg gtcaaggtat gagtttggct caggctcttc aagaccgtaa acgcggtgct
     2881 gccccaaaat caatccagtc aagcggtctt ccatttggtc aatttacagc tggcgcaaaa
     2941 ccagcatcta gcgaggcaaa ttggtccatt ggtgatattg ctctccacaa gaaatgggga
     3001 gagggaaccg ttctggaagt ttcaggtagc ggtgctaggc aggaattgaa aatcaatttc
     3061 ccagaagtag gtttgaaaaa acttttagcc agtgtggctc caattgagaa aaaaatctaa
     3121 ttttccatcc ttctcacgaa taataaagtg aggaggattt ttatgtacag tatttcattc
     3181 caagaagatt cactattacc aagagaaagg ctggccaagg aaggagttga agcgcttagt
     3241 aaccaagagt tgctagctat tttactcagg acaggaacac gtcaagttag cgtttttgaa
     3301 attgcccaaa aagtcttgaa caatctttca agcctaacgg atttgaaaaa aatgaccctg
     3361 caggaattgc agagtttgtc tggtattggg cgtgttaagg ccatagaatt acaagctatg
     3421 attgaactgg ggcatcgtat tcacaaacac gagactcttg aaatggaaag tattctcagc
     3481 agtcaaaagt tggccaagaa gatgcagcag gaattagggg ataaaaaaca agagcacctg
     3541 gtggcactct atctcaatac tcaaaatcaa atcatccatc agcagaccat ttttatcggg
     3601 tctgtaactc gtagtatcgc tgaaccgcga gagattcttc actatgcaat caagcatatg
     3661 gcgacttctc ttatcttggt ccacaatcat ccttcaggag cggtagcgcc tagccaaaat
     3721 gatgatcatg tcactaaact tgttaaagaa gcctgcgaat tgatggggat tgttctcttg
     3781 gaccatttga ttgtctctca ttctaattac tttagttatc gtgaaaagac agatttaatc
     3841 taaagttcat taacgacata gtcaaagagt tttttatctt tgggacgatt ttcaaaaaga
     3901 agttctggat gccattggac accgagaaag gcgacatcat ccgtactcat gacagcctca
     3961 atgataccat ctttaggatc atgagccaca acttttaaat ttggtgctaa gtccttgatg
     4021 ctctggtggt ggaaggagtt gatatgagag atttctccat agatttcttg gagaacggta
     4081 tctggttctg ttaccaagcg ttgagttgtg tactcaacag aagaatcctg ccaatggtct
     4141 tcgatatctt ggtacaaagt tccacccatg gcaacgttaa agagttgggt accacggcag
     4201 acagagaaaa tgggcttttt ctgtttaata gcttccttga tgagggtcag ttcgaagata
     4261 tctctttgaa ggtgatagtc atcgctatca atggttttgg gttcgccata aaattttgga
     4321 tcgacatttt gcccacctgt caagatgagc ttgtcaatca aactgatata gtggcaggcc
     4381 atttcttgat caccaatcgg taggatgatg ggaatccctc cagcatcttt aacgccttca
     4441 acaaagcctt ttgctgcgta gctcatcatg atgtcatcat ctggatgagc tttttcgttt
     4501 cctgtaatcc caataactgg ttttttcata aaatgatttt cgctttctaa tcctcttttc
     4561 gcatgaagta gaggagggtt tggagttcac ttgtcaaatc gacatactga acgaccacgt
     4621 cttttggtaa atgcagatgg actggtgaaa aactgagaat tcctttcaca ccagcatcaa
     4681 ccaagagatt agcaacctct tgtgacttga cgctgggaac agttaggata gcagtcttca
     4741 catcagcatc cttgatttta tccttgatct gagaaatccc gtaaatggga atcccgtcag
     4801 gagtttgggt accgacttca ggatggtcgt ctaggtcaaa ggccatgata atcttcatct
     4861 tgttacgttc gtggaagcgg tagtggagaa gggcatggcc catatttcca ataccaacca
     4921 gcatgacatt ggtaatagag ttgtcattga gcaaatcggc aaaaaatgtc attagttttt
     4981 tgacatcata gccaaaacca cgacgaccaa gttcaccaaa ataggaaaaa tcacgacgta
     5041 cggtcgctga atcaataccg atagcctctg caatttgctt agagttggca cgttcaatct
     5101 tttctgcatg aaatctctta aaaattcgat agtagagaga gagtcttttt gctgtagctt
     5161 ttggaatagc aaactgttta
//
LOCUS       DAVMMR010000089         4881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMMR010000089 DAVMMR010000000
VERSION     DAVMMR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.089"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            202..420
                     /locus_tag="VYS74_001912"
     CDS             202..420
                     /locus_tag="VYS74_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV3789899.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1465..2457
                     /locus_tag="VYS74_001913"
     CDS             1465..2457
                     /locus_tag="VYS74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3789900.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2459..3277
                     /locus_tag="VYS74_001914"
     CDS             2459..3277
                     /locus_tag="VYS74_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV3789901.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3279..4064
                     /locus_tag="VYS74_001915"
     CDS             3279..4064
                     /locus_tag="VYS74_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789902.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4162..4380
                     /locus_tag="VYS74_001916"
     CDS             4162..4380
                     /locus_tag="VYS74_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV3789903.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4365..4526
                     /locus_tag="VYS74_001917"
     CDS             4365..4526
                     /locus_tag="VYS74_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3789904.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1402 a          800 c         1027 g         1652 t
ORIGIN      
        1 gagcaacctg cggctagttt cctagtttgc tctttggttt tcattgagta taacacattg
       61 ttagaagttg gtttaaattt cctaatcagt ttgttcacat ttaccttcga tatattatat
      121 cccatagtta aggttggtca tacagatgat tatagccatg gagccgtaaa acttagtgtt
      181 tctttagttg acaaagatgc catgaaaaaa atatctgtaa ctgtaatagg atattttgaa
      241 ataaatatag atgaaaatat caccgatatt ctatacgtaa atggtactgc tattctttat
      301 ccttatttac gttctattgt ttcaatagtt tcggcaattg atagcagtga agcaatgttg
      361 ctacctacca ttaatgtttt agagttacta gataaatctc aaccttttga agaagaataa
      421 tttattagct cactaaattg agggtaagga aaagtaaaag cagtaagaaa aatgtcttgc
      481 attatacagc aactttttgg gaatgagtag atggattgaa taaaatttga ttaagagtgg
      541 atgatttatc tgtagattat tattggacag ttagtcttga agtagtctaa gaattaggtt
      601 ataatcagta gaagccttgc taataatgag gaggttagtt tatgtatagt agactgaatc
      661 taaaatagta cgaaacaatt gctaaaacat ttatagaaat taattttact ttcccaatcg
      721 atttgttctc atcttatttc aatccgctat atattatggt atcgaatctt catcagaatg
      781 ataaaattaa tcaattaata tctgattaca aacagaatat gaaagctttt tatatcacta
      841 ttgaaaaatt tatacgagat gatgaaagcc ttaagtgtta ttttacaaag gttatttcaa
      901 gtcgttccaa ggtaacaagt ctagatcaga ttgaagctga taaaacgata caaagaaaat
      961 attcaagtga gctaaaaaaa tttattggat tttataatga gattatttgt gaggaaaata
     1021 gtttcctaca tgtacgaaag aggtggtcga gttggtttag gtagtcggtg cgtgagttga
     1081 taattctcag ggtatggact tctttttcat gaatgaggta aaagagcagg tattgtttag
     1141 agacaatcat tctgagcata ttttctggat agagggagta tccgatttta tgatcaaagt
     1201 taataccgcc ctctggtgag aagatgagta ggttggtaat ttaaactatt aaacagaatt
     1261 tttgattaaa agtattattt catgagagaa atcctaattt cacaatctat aggcaaacgc
     1321 ttgcatttcg ttttttattg gactataata ggttggtata aagccttctg tagtaataaa
     1381 atgtagaagg tgtagaaagt aaggatttag aatatttgta gttaaaaaca caatgttgct
     1441 attccttacg atagggagat agatatggca atgatagaag tggaacatct tcagaaacat
     1501 tttgtgaaga ttgttaagga accgggcttg aagggggctt tgcgctcctt tattcatcct
     1561 gaaaagcaga cctttgaagc ggtcaaggat ttgacctttg aggttccaaa agggcagatt
     1621 ttaggattta tcggggcaaa tggtgctggg aagtcgacaa ccattaaaat gctgacagga
     1681 attttgaaac caacatctgg tttttgtcgg attaacggca agattcccca ggacaatcgg
     1741 caagattatg tcaaagatat tggcgtagtc tttggacaac gcacccagct atggtgggat
     1801 ttggctctgc aagagaccta cactgtctta aaagagattt atgatgtgcc aaactcgctc
     1861 tttcataagc gtatggactt tttgaatgaa gtcttggatt tgaaggactt tatcaaggat
     1921 cccgtgcgga ctctttcact gggacaacgg atgcgagcgg atattgcggc ctccttgctc
     1981 cacaatccca aggttctttt tttagatgag ccgaccattg gtttggacgt ttcggttaag
     2041 gataatattc gtcgggcaat tactcagatc aatcaagagg aagaaactac cattcttttg
     2101 accactcacg atttgagtga tattgagcaa ctttgtgatc ggattttcat gattgacaag
     2161 gggcaaaaga tttttgatgg aacggtgagc caactcaagg agacctttgg taagatgaag
     2221 actctctctt ttgaactgct accaggtcaa agtcatctcg tctctcacta tgaaggtctg
     2281 tctgatatga ccattgatag acaaggaaac agcctcaaca ttgaatttga tagttctcgc
     2341 taccagtcag ctgacattat caagcaaacc ctatctgatt ttgaaatccg cgatttgaag
     2401 atggtggata cggatattga ggatattatc cgtcgcttct accgaaagga gctctaggat
     2461 gatcaaattg tggagacgtt ataaaccctt tatcaatgca ggggttcagg agttgattac
     2521 ttaccgagtc aactttattc tctatcggat tggcgatgtc atgggggctt ttgtggcctt
     2581 ttatctctgg aaggctgtct ttgattcttc gcaagagtct ttgattcagg gcttcagtat
     2641 ggcggatatc accctctaca tcatcatgag ttttgtgacc aatcttctga ctagatccga
     2701 ttcgtccttt atgattgggg aggaggtcaa ggatggctcc attatcatgc gtttgttgcg
     2761 accagtgcat tttgcggcct cctatctttt caccgagctt ggttccaagt ggttgatttt
     2821 tatcagcgtt ggccttccat ttttaagtgt cattgtcttg atgaaaatca tatcgggtca
     2881 aggtattgta gaggtgctag gattaactgt cctttatctt tttagcttaa cgctcgccta
     2941 tctgattaac tttttcttta atatttgctt tggattttca gcctttgtgt ttaaaaatct
     3001 ttggggttcc aacctactta agacttccat agtggctttt atgtcgggga gtttgattcc
     3061 cttggcattc tttccaaagg ttgtttcaga tattctctcc tttttgcctt tttcgtcctt
     3121 gatttatact ccagttatga tcattgttgg aaaatacgat gccagtcaga ttcttcaggc
     3181 actccttttg cagttcttct ggctcttagt gatggtggga ttgtctcagt taatttggaa
     3241 acgggtccag tcctttatca ccattcaagg aggttagtat gaaaaaatat caacgaatgc
     3301 atctgatttt tatcagacaa tacatcaaac aaatcatgga atataaggtg gattttgtgg
     3361 ttggtgtctt gggagtcttt ctggctcaag gcttgaatct cttgtttctc aatgtcatct
     3421 ttcaacatat tccattccta gaaggctgga cctttcaaga gatagctttc atttatggat
     3481 tttccttgat tcccaaggga atggaccatc tcttttttga caatctctgg gcactagggc
     3541 aacgcctagt ccgaaaagga gagtttgaca agtatctgac tcgtcccatc aatcctctct
     3601 ttcacatcct agttgaaacc tttcagattg atgccttggg tgaactctta gtcggtggta
     3661 ttttattggg aacaacagtg accagcattg tttggactct tccaaaattc ctgcttttcc
     3721 tagtttgtat tccttttgcg accttgattt atacttctct taaaatcgca acagccagta
     3781 tcgccttttg gactaagcag tcaggcgcca tgatttacat cttctatatg ttcaatgact
     3841 ttgctaagta tccgatttct atttacaatt ctcttcttcg ttggttgatt agctttatcg
     3901 tgcctttcgc ctttacagcc tactatccag ctagctattt cttacaggaa aaggatgtgt
     3961 tctttaacgt aggaggtttg atgttgattt ctctggtttt ctttgttatt tcccttaaac
     4021 tttgggataa gggcttagat tcctacgaaa gtgcgggttc gtaaaagcta aagtaagact
     4081 aaaatcaaga aaggaactta tgatgtttgt aattgaagaa gtcaaggatg aaaatcaaaa
     4141 aaggcagttg tcgttgaggt tttgaaggat ttgccagaat ggtttggaat cccagaaagc
     4201 acacaagcct atatagaagg aaccacgaca ctgcaagttt ggaccgccta tcaggagagt
     4261 gatttgactg gatttgtaag gttatcctat tcgagtgaag attgtgcaga gattgattgt
     4321 ctcggcgtaa aaaagcttat caaggtagaa aaattgggag ccaattgctt gctactttag
     4381 agagtgaagc tcgtaaaaaa gttggttatc tgcaggtcaa aacagtggca gaaggttcta
     4441 ataaagatta tgatcgaaca aatgactttt atcgaggtct tggctttaaa aagttagaga
     4501 ttttttctca gctatggaat ccgtaaaatc cttgtcagat tttgattaaa aagcttgaat
     4561 aatattactt gacatctatt ctcagagtgc catactgtaa gtgtaatcgc cgatttagct
     4621 cagttggtag agcaaggcac tcgtaaagcc taggttatag gtagataaac gactgaggag
     4681 ttgaaaaaat agataggtag aagataaccg ttaagcctta ttcttagcgg ttatttatat
     4741 tgtttaatag cgctgatatt tcatcaatta tgcctgtttt cgtgtttctg gtagttgttc
     4801 aagtttattg ctactatttt tgatggtatg aatgtgctta taatgtatcc cggttaacga
     4861 aagttttgga cttatactca t
//
LOCUS       DAVMMR010000090         4810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMMR010000090 DAVMMR010000000
VERSION     DAVMMR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.090"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..493
                     /locus_tag="VYS74_001918"
     CDS             <1..493
                     /locus_tag="VYS74_001918"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789905.1"
                     /translation="FLKNFNSLKHLAPVYIDETGIDRYLYRPYAGAPRGEKVYEKISG
                     RRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFH
                     PKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFF
                     KTR"
     gene            833..1087
                     /locus_tag="VYS74_001919"
     CDS             833..1087
                     /locus_tag="VYS74_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789906.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            1198..2304
                     /locus_tag="VYS74_001920"
     CDS             1198..2304
                     /locus_tag="VYS74_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV3789907.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            2262..2939
                     /locus_tag="VYS74_001921"
     CDS             2262..2939
                     /locus_tag="VYS74_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3789908.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            3163..4032
                     /locus_tag="VYS74_001922"
     CDS             3163..4032
                     /locus_tag="VYS74_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3789909.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
BASE COUNT         1633 a          870 c          976 g         1331 t
ORIGIN      
        1 atttcttaag aattttaata gtttaaagca cctagcacct gtttatattg atgaaacagg
       61 aatcgaccgc tatctctatc gtccttatgc aggggctcct agaggggaga aagtctatga
      121 aaagattagc ggacgtcgtt ttgagcgaac ttcaattgtt gcaggacaag tagacggaga
      181 gtttatagct cccatgattt acaagaaaag catgacaagc gatttctttg tggagtggtt
      241 caaaacgcaa ctcctacctg ctttgaagac acctcatgtt attgtcatgg gcaatgctgg
      301 ttttcatccc aagaacattt tggatgaact ctgcatccaa gataaacact ttttcttacc
      361 tctaccacct tattcaccgg atttgaatcc tattgagcaa gcttgggcta tcttgaaaaa
      421 gaaagtgacg gatttattaa gggaagttcc aactattttt gaatgtttgg aatgcttttt
      481 taaaactaga tgactataac ggttctaaag gaacctatcg agtagtcatt aaaactaagg
      541 atactgctgg ttaagagaag acggtataca atcaaaccat tcaccgtgta gccgaaatcg
      601 ttcagaatga agacttgtat aagaaaggtt tgaatgttga acttgcgcac caacaaatta
      661 agggattttt tgaagcagag tttaaaaatc gtattaatgg agttcttaat actaaaataa
      721 aaaatagtac attaaatcgt gtaaataaaa aaactataca ccagagcaac aaaaactcca
      781 tgatcaattt gaagcagaag caacggaaga tgctaaaaaa caaggcgata ttgtgttgaa
      841 tgttgaccag gatttcatga gcatatctaa gtctaataaa agtggttcag actggaagaa
      901 aactttcaca gtgaggataa ccaataggct agcaaatgac ttgaataatg tcttgaaaca
      961 ggctgataaa gatactccta ataccccaac ttggctaaac tcagctgctt ctaaagctaa
     1021 agatgatgac agagtatata aactactgaa gactcttata ccaggagaaa attacctatc
     1081 atgttaagga taatcagcta gaagtagaaa cagataaata cacatatact gccgctagaa
     1141 atggtagtaa ggaagttggt attcaagagt cagatatagc agcaactcta agtgccgatg
     1201 aatataattc taatcgccaa acttttgaga gagaatacaa atacaaaagc aaatgccctt
     1261 aataatggtt gggctagatc tggttctgaa gagttcaaaa agttctccca ctttgtaggg
     1321 gtagacaaag ggattgtgcg aacgaatgta ctgactggta aaaaactatc tgataagatt
     1381 aggaaagaag tgggctctgg agatagcaaa ctaggaaaag gcggctattt ctctactggg
     1441 gatgttctat taggaaaaga tgttgtttct tataccgtac aagtattttc agagaataat
     1501 gaaagagtag gagtaaacac tcaaagtcac cgtgttcagt ataatctccc aattctagct
     1561 gacttttcag tcatccaaga tactgtggaa ccatcacgaa ccgttgttga aaaaatcatt
     1621 ccaaaactaa atattcccga agaagagaaa gggaaaataa ccgaagaaat caagaaaaag
     1681 aaaaaaacct cagaattggc agaactaatc tcagaaaatg tgaaagttcg ctatgttgat
     1741 gaacaagggc gtttgctatc attgaaaaat gatactggaa ttggagaaaa agaaagtgac
     1801 ggaacctaca ttaccaataa aaaacaactg attggtacca gctacaatgt cacagataaa
     1861 aaactcagta gcatgactac tactgacgga aaatattata cttttaaaga agcagataca
     1921 aattctgcaa gtttaactgg gaatattgta agcgaaggta gaacagtgac cttagtttat
     1981 agagaaagcg aagcgccaac cactgctaca gtaacagcca attactataa agaaggtagc
     2041 caagagaagt tggcagagtc tgttataaaa gctgatttag cgataggttc tgagtatacc
     2101 acagaatcaa aaactattga agggaaaaca acaactgagg acaaagaaga ccgagttatc
     2161 acaaggaaaa caacatacac cttggtagca actcctgcaa atgcgtacca gaagacggtg
     2221 caacagttga ctattactac cgtgagaatg ttgaggaaac agtggttccc aaaacagcaa
     2281 cctctactga gacgaagact ataacgcgta tcattcatta cgttgataaa gttacgaacc
     2341 aaaatgtaaa agaagatgtt gttcaacctg taaccttaag ccgtacaaaa attgagaaca
     2401 aggtcacggg agttgtaacc tacggtgaat ggacaacagg aaactgggac gaggttgtat
     2461 ctggtaagat tgacaagtac aaagatccag atattccaac agttgaatca caagaagtta
     2521 cgtcagactc tagtgataaa gaaataacgg taaggtatga ccgtttatca acaccagata
     2581 aaccaactcc agaaaaacca gaaattccaa gtcctcagga accaggtact ccaggtgaac
     2641 cgactccaga aaaaccaatc ccacaaccaa atccagagca tccaagtgtt ccgacaccaa
     2701 acccagaact accaaatcaa gagactccaa taccagagcc aactccagaa ccaggtactc
     2761 caaaaactga aactccagtg aatccagacc cagaagttcc gacttatgag acaggtaaga
     2821 gagaggaatt gccaaacaca ggtacagaag ctaatgctac cttggctagt gctggtatca
     2881 tgaccttgtt agctggtcta ggattaggat tcttcaagaa aaaagaagat gaaaaataat
     2941 agattttaga atctaggaac caggaaaagc tcacagatgt gggctttttt cctggttttg
     3001 agaacgaggt ctttcgtgaa gaataaaaac gcttacaagt ccgttgaact gggaaactat
     3061 gaatcctatt tttttaaaaa tatttccaga aatcagttgc taaatagggt aaaaattgat
     3121 aaaataagga atatctaaaa aattttaagg agaatctagc aaatggattt cacatgggca
     3181 ctgaagtatg ccactgaatt tttgggaact gccattttga tcattcttgg gaatggtgca
     3241 gttgccaacg ttgaacttaa aggtacgaaa ggtcaccaaa gtggctggat cgtcatcgct
     3301 gttggttatg gtatgggggt tatgatccca gccttgatgt ttggtaacgt atctgggaat
     3361 cacatcaacc ctgctttcac tctagggctt gcagttagcg gtcttttccc ttgggcacaa
     3421 gtggtacctt atattatcgc gcaagtcttg ggagctatct ttggccaagc cttagttgtg
     3481 gcaacatacc gtccattcta cttgaaaact gaaaacccaa ataacatctt gggaactttc
     3541 tcaactattt caagtattga ccatggtaca aaagaaagtc gctatgcagc aactgtcaat
     3601 ggtttgatta atgagtttgt tggttcattt gttttgttct ttgcagctct tggtttgact
     3661 aaaaacttct ttggtgctga agtgcttcaa ttcatgaaac aaaaggcaat agaagcagga
     3721 caaacagttg atttttctga cttggctatt aaagcacagg tggctccaca cactgcttca
     3781 ggactttctg tggctcactt ggcacttgga ttcctcgtta tggctttggt aacatcactt
     3841 ggaggaccta caggacctgc cttgaaccca gcccgtgact tgggaccacg tctccttcat
     3901 gctttccttc caaaatcagt tcttggtgag cataaaggcg attcaaaatg gtggtattct
     3961 tgggtaccag tagtagcacc tatcgtagca gcaattgcgg cagtagctgt attcaaattc
     4021 ctttatctct aagaaatagc tcctttaaca tttgagtgag caccatctat aagtaagaga
     4081 ggatcagact ggttctctct ttttgatttt ttagggaaat gaaagaaact ctaaacaaac
     4141 tcctctccag cagtggttta gaagtctcag tgggctattc cagcttcaat ggactatagt
     4201 aggttgcagt tgaaataata gacccttgtt tctaaaacat tgtgagaaat tggtttgaat
     4261 tctccaatca aattgtgcag ttttcattct actatatatt atcggagatg ggttccctat
     4321 cttgtaagtc tgctttatag tgggttgaag ttggaatagt cctcccttct ttctcaaaca
     4381 ttgtgaggaa ttgatttacc ttcctcaaca aaatgttcag tttctatttc attttgctat
     4441 aaaataagcg attagggggg ctattcttcg acctacattg actctgctga gtcctatgat
     4501 tgttatcgtt ttatctgcaa ttttatactc aatgaaaatc aaagggcaaa ctaagaagct
     4561 agccgcaggt tgttcaaaac acagttttga ggttgtatag tagattgaaa ctagaatagt
     4621 acacatctac ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttgctct
     4681 attcttgttt cattttacta tataaaccag agactgttta cattttcagc aagtgagtgg
     4741 atggataatg ctgaaaactc cttgaaggat aagtctattt agtactttct attaattagt
     4801 taaattttta
//
LOCUS       DAVMMR010000091         4690 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMMR010000091 DAVMMR010000000
VERSION     DAVMMR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.091"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            484..1137
                     /gene="rr06"
                     /locus_tag="VYS74_001923"
     CDS             484..1137
                     /gene="rr06"
                     /locus_tag="VYS74_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV3789910.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            1134..2465
                     /gene="hk06"
                     /locus_tag="VYS74_001924"
     CDS             1134..2465
                     /gene="hk06"
                     /locus_tag="VYS74_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV3789911.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2548..3075
                     /locus_tag="VYS74_001925"
     CDS             2548..3075
                     /locus_tag="VYS74_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV3789912.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            3154..>4690
                     /gene="pspC"
                     /locus_tag="VYS74_001926"
     CDS             3154..>4690
                     /gene="pspC"
                     /locus_tag="VYS74_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV3789913.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLVMGSVVHATENEGTT
                     QAPTSSNRGNESQAEHMKAAKQVDEYIEKMLQLDRRKHTQNVGLLTKLGAIKTEYLRG
                     LSVSKEKSTAELPSEIKEKLTAAFKQFKKDTLKPEKKVAEAEKKVAEAKKKAEDQKEE
                     DRRNYPTITYKTLELEIAESDVEVKKAELELVKVKANEPRDEEKIKQAEAEVESKKAE
                     ATRLKKIKTDREKAEEEAKRRVDAKEQDESSKRRKSRVKRGDLGEQATPDKKENDAKS
                     SDSSVGEETLPSPSLKPGKKVAEAEKKVEEADKKAKAQKEEDRRNYPTNTYKTLELEI
                     AESDVEVKKAELELVKEEAKEPRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEE
                     AKRKAAEEDKVKEKPAEQPKPAPAPQPEKPAPKPENPAEQPKAEKPADQQAEEDYARR
                     SEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNNDGSMATGWLQNNGSWYY
                     LNAN"
BASE COUNT         1681 a          705 c          990 g         1314 t
ORIGIN      
        1 aaactaaaac ctatagcttc taaacgatcc gtggttttca ccattcaaca caaaattcat
       61 atgtttatta ccctccgtcg tatttgtctt agagcgtgtg tagtagaaaa agagcagtct
      121 tatctgaaat ttttattctt tcaaaagaga cctgtttctt ttttgcatgt caaatccgtt
      181 ctagctggta tttgaaaaat caaactaata ttcaatgaaa atcaaagaac aaactaggaa
      241 gctagccgca ggttgctcaa aacactgttt tgaggttgta gatagagctg acgtggtttg
      301 aagagatttt cgaagagtat aagctgcaag atgaatgatt ttcttgtatt gacgttgttg
      361 ttgacaaaaa gtagcggata aatgaaatcc attccattat catagatgat aggctggtag
      421 gaaattttca aatagcatac aggaaataga tgtatggagt tctggtagta gaaagggaga
      481 gagatgaaca ttttagttgc agatgacgag gaaatgatta gagaaggaat tgcagcattt
      541 ctgacagaag agggttatca tgtcattatg gctaaggatg gacaagaggt cttggaaaaa
      601 tttcaagatc tccctatcca tctcatggta ctggatttaa tgatgcctag gaagagtggt
      661 tttgaagtgt taaaagaaat caatcaaaag cacgatattc ctgtcatcgt cttgagtgct
      721 ctgggagatg aaactactca gtcacaggta tttgatctct atgctgatga tcatgtgaca
      781 aaaccttttt ctttggtact gcttgtcaag cgtattaagg cgcttatcag acgttactac
      841 gtcatagagg atatttggcg atatcaggat gtaacagtgg attttacctc ttacaaagca
      901 cattataaaa atgaagaaat tgatctcaaa ccaaaggaat tactggtact aaagtgtttg
      961 attcagcata aaaatcaagt tttaagtaga gagcagatat tggaagaaat ttcaaaagat
     1021 gtagctgatt taccttgtga tagggtcgtt gatgtctata ttcgtactct tcgcaaaaaa
     1081 ttagctttag attgtatcgt gactgtgaaa aatgttgggt ataagattag cttatgataa
     1141 aaaatcctaa attattaacc aagtcctttt taagaagttt tgcaattcta ggtggtgttg
     1201 gtctagtcat tcatatagct atttatttga cctttccttt ttattatatt caactggagg
     1261 gggaaaagtt taatgagagc gcaagagtgt ttacggagta tttaaagact aagacatctg
     1321 atgaaattcc aagcttactc cagtcttatt caaagtcctt gaccatatct gctcacctta
     1381 aaagagatat tgtagataag cggctccctc ttgtgcatga cttggatatt aaagatggaa
     1441 agctatcaaa ttatatcgtg atgttagata tgtctgttag tacagcagat ggtaaacagg
     1501 taaccgtgca atttgttcac ggggtggatg tctacaaaga agcaaagaat attttgcttt
     1561 tgtatctccc atatacattt ttggttacaa ttgctttttc ctttgttttt tcttattttt
     1621 atactaaacg cttgctcaat cctctttttt acatttcaga agtgactagt aaaatgcaag
     1681 atttggatga caatattcgt tttgatgaaa gtaggaaaga tgaagttggt gaagttggaa
     1741 aacagattaa tggtatgtat gagcacttgt taaaggttat tcatgagttg gaaagtcgta
     1801 atgagcaaat tgtaaaattg caaaatcaaa aggtttcctt tgtccgcgga gcatcacatg
     1861 agttgaaaac ccctttagcc agtcttagaa ttatcctaga gaatatgcag cataatattg
     1921 gagattacaa agatcatcca aaatatattg caaagagtat aaataagatt gaccagatga
     1981 gccacttatt agaagaagta ctggagtctt ctaaattcca agagtggaca gagtgtcgtg
     2041 agaccttgac tgttaagcca gttttagtag atattttatc acgttatcaa gaattagctc
     2101 attcaatagg tgttacaatt gaaaatcaat tgacagatgc taccagggtc gtcatgagtc
     2161 ttagggcatt ggataaggtt ttgacaaacc tgattagtaa tgcaattaaa tattcagata
     2221 aaaatgggcg cgtaatcata tccgagcaag atggctatct ctctatcaaa aatacatgtg
     2281 cgcctctaag tgaccaagaa ctagaacatt tatttgatat attctatcat tctcaaatcg
     2341 tgacagataa ggatgaaagt tccggtttgg gtctttacat tgtgaataat attttagaaa
     2401 gctatcaaat ggattatagt tttctccctt atgaacacgg tatggaattt aagattagct
     2461 tgtagacaga ttagtttttt attaaagttc atatagggtt aacataagtg tgctattctt
     2521 tgtgtagata aaagaaagga tactaatatg gtattagcga ttattttagt aacattcctt
     2581 attcgattga tttttttaaa gcgttcgata gagaatgaga aacgaatcct tagcaatggc
     2641 gggaaagaat ttggagttga gaatacaaaa cgattaacta tggctcatat tgttttttat
     2701 ctctcttgct tggttgaggc aatggtgcac aagacaattt ttgatggcat gggcatggtt
     2761 ggtttagtct tgcttatttt ttctatgctg atgttgatgt tggtgattca cttgttggga
     2821 gatatttgga cagtgaagct tatgcttgtc aataatcaca aatatgtaga tcatatcttg
     2881 tttaggacag taaaacaccc taattacttt ttaaatattt tacctgagtt gattggcttg
     2941 accttgttga gtcatgccta tatgactttt gttttagttt ttccagttta tgcagttatt
     3001 ttgtatcgac gaatagctga agaggaaaag ctattacatg aagttataat cccaaatgga
     3061 agcataaaga gataaataca aaattcgatt tatatacagt tcatattgaa gtgatatagt
     3121 aaggttaaag aaaaaatata gaaggaaata aacatgtttg catcaaaaag cgaaagaaaa
     3181 gtacattatt caattcgtaa atttagtatt ggagtagcta gtgtagctgt tgccagtctt
     3241 gttatgggaa gtgtggttca tgcgacagag aacgagggaa ctacccaagc acccacttct
     3301 tctaataggg gaaatgaaag tcaggcagaa catatgaaag ctgctaaaca agtcgatgaa
     3361 tatatagaaa aaatgctcca attagataga agaaaacata cccaaaatgt cggcttactc
     3421 acaaagttgg gcgcaattaa aacggagtat ttgcgtggat taagtgtttc aaaagagaag
     3481 tcgacagctg agttgccgtc agaaataaaa gaaaagttaa ccgcagcttt taagcagttt
     3541 aaaaaagata cattgaaacc agaaaaaaag gtagcagaag ctgagaagaa ggtagcagaa
     3601 gctaagaaaa aagccgagga tcaaaaagaa gaagatcgtc gtaactaccc aaccattact
     3661 tacaaaacgc ttgaacttga aattgctgag tccgatgtgg aagttaaaaa agcggagctt
     3721 gaactagtaa aagtgaaagc taacgaacct cgagacgagg aaaaaattaa gcaagcagaa
     3781 gcggaagttg agagtaaaaa agctgaggct acaaggttaa aaaaaatcaa gacagatcgt
     3841 gaaaaagcag aagaagaagc taaacgaaga gtagatgcta aagagcaaga tgaatcatca
     3901 aagaggcgaa agagtcgggt aaaacgagga gatcttggag agcaagcaac acctgataaa
     3961 aaagaaaatg atgcgaagtc ttcagattct agcgtaggtg aagaaactct tccaagccca
     4021 tccctgaaac caggaaaaaa ggtagcagaa gctgagaaga aggttgaaga agctgataaa
     4081 aaagccaagg ctcaaaaaga agaagatcgc cgtaactacc caaccaatac ttacaaaacg
     4141 cttgaacttg aaattgctga gtccgatgtg gaagttaaaa aagcggagct tgaactagta
     4201 aaagaggaag ctaaggaacc tcgaaacgag gaaaaagtta agcaagcaaa agcggaagtt
     4261 gagagtaaaa aagctgaggc tacaaggtta gaaaaaatca agacagatcg taaaaaagca
     4321 gaagaagaag ctaaacgaaa agcagcagaa gaagataaag ttaaagaaaa accagctgaa
     4381 caaccaaaac cagcgccggc tcctcaacca gaaaaaccag ctccaaaacc agagaatcca
     4441 gctgaacaac caaaagcaga aaaaccagct gatcaacaag ctgaagaaga ctatgctcgt
     4501 agatcagaag aagaatataa tcgcttgact caacagcaac cgccaaaaac tgaaaaacca
     4561 gcacaaccat ctactccaaa aacaggctgg aaacaagaaa acggtatgtg gtacttctac
     4621 aataatgatg gttcaatggc gacaggatgg ctccaaaaca atggttcatg gtactatcta
     4681 aacgctaatg
//
LOCUS       DAVMMR010000092         4683 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMMR010000092 DAVMMR010000000
VERSION     DAVMMR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.092"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(145..777)
                     /locus_tag="VYS74_001927"
     CDS             complement(145..777)
                     /locus_tag="VYS74_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3789914.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(799..1671)
                     /gene="sdaAA"
                     /locus_tag="VYS74_001928"
     CDS             complement(799..1671)
                     /gene="sdaAA"
                     /locus_tag="VYS74_001928"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV3789915.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1680..2351)
                     /gene="sdaAB"
                     /locus_tag="VYS74_001929"
     CDS             complement(1680..2351)
                     /gene="sdaAB"
                     /locus_tag="VYS74_001929"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV3789916.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2593..3165
                     /locus_tag="VYS74_001930"
     CDS             2593..3165
                     /locus_tag="VYS74_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3789917.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3218..3538)
                     /locus_tag="VYS74_001931"
     CDS             complement(3218..3538)
                     /locus_tag="VYS74_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV3789918.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            4302..4655
                     /locus_tag="VYS74_001932"
     CDS             4302..4655
                     /locus_tag="VYS74_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV3789919.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT         1417 a          839 c         1007 g         1420 t
ORIGIN      
        1 tgttttgagc tgacttcgtc agttctatct gcaacctcaa aacagtgttt tgagcagccc
       61 gcagctagtt tcctagtttg ctctttgatt ttcattgagt attacttcat tttcttctga
      121 aatggaattg ttacccagtc tatgctattg aaaatacgcc aaaacttcta agggtttgtg
      181 agcgatataa tcaggttgat agtttagtag atctgcttgc tctccaaatc cccaagtgat
      241 ggccaatttc tgaatacctg tttctcgagc tcccagcata tcaaacttgg tatctccgat
      301 gatgatggct tgttctggtg ctagttgatg tgtctgcaag gcttggtgaa tgacatctgc
      361 cttatggggt gcttcagggc tagaaccata aatgccatca aagaaatgat ggatttccaa
      421 gttttttgcc atgtcttgag cagtagatgt atcctttgtc gtggtgatgt agagtggata
      481 actgctcgat aactcctcaa gcaagtctat aatctgagga aagagttgag cttcatagat
      541 gccttttgcc ttatagtaag aacgatatat ctgcacggct tcagaaattt ggtctttgga
      601 caggcaggtc gcaaaactac tttcgagagg tggtcccata aaaccacgaa tagttttggc
      661 atcagggcta ggcaccccca gctctttaaa ggtataggta aaggcattgt gaatcccgat
      721 agaactatca acgagggttc catccaaatc gaaaaaaatt gctgtgatag aggtcatggt
      781 ttctcctatt tgataagctt attctccgaa aatttctttt tggaggcgac gaccagtagg
      841 ggtggtagcg agtccacctt cagctgtttc acgaaaggca gttggcatgc ttgctcctac
      901 ttggtacatg gcatcgatca cttcatccac agggatttta gattcgatac ctgccaaggc
      961 catgtctgct gcgatgaaag caaagctagc tcccatggca ttacgtttga cacagggaac
     1021 ttcgaccaaa cctgcaacag ggtcacagat gaggcctagc atatttttaa tgacaaaggc
     1081 aatagcttga ctggcctgat aaggtgttcc acctgcagcc agagtcaagg cggcagcact
     1141 catagcagag gctgaaccaa cttcagcttg acacccaccc tcagcacctg agatggaggc
     1201 attgtttgcg atgactagtc caaaggcacc agcagcaaag aggaaatcca attgttgctc
     1261 gtggctgagg tctaattttt caatagcagc agtgagaacg gaagggagac agccagcact
     1321 tccagcggtt ggagtggcac agaccaagcc cattttggca ttgtgttcat tgactgcgat
     1381 agcatttcgg gcagcagaga gaatcgtgta atctgataaa gttttttcgt ttttgatgta
     1441 gtgatccaat ttggcagcat ctccacctgt caggccacta cgagatttat tttcattgag
     1501 gccaagttgg acagaggctt tcataacttc cagattgcgt tccatgagaa ggaagacttc
     1561 ttcacgttcg cgaccggtca attcaaactc tgttgtaatc atgagttctg cgacatttcc
     1621 ttgaaagtct agatctgctt gctcgaccaa ttctttgata gaataaaaca tgcttcctcc
     1681 tatttaaaga aattgacatt gtggagatga gggatttttc gaatttcttc gatagcctca
     1741 tcacagttgc gactgtcaac ttcgataatc ataatggctt tttcaccagc tttttcacga
     1801 gtgacattca tctgggcgat attgatacca tagcgggaaa gcgcctctgt aacaagggca
     1861 atcatacctg gaatatcttg atgaacgatg atgatagtcg gtgtattcat attgagagag
     1921 acggcaaaac cattgagttc ggttacctga atatttcctc caccgataga aataccagtc
     1981 acgctgatgg tcttgtgggc atttttaaca gtaattttag tggtgttagg gtgaggggca
     2041 ttgctgtctt tctgaatggt ccagacaatc ttgataccac gcttgtgggc aatttccaga
     2101 ctatttggaa tttcaggatc atctgtatcc attcctaaaa tacctgcaac aagggctagg
     2161 tctgttccgt gaccacgata ggtcttggca aatgagttaa aaagttggaa ttcaacttct
     2221 gtcggagtat catcaaaaat ggaagagaca atcttcccaa tacgaacagc accagcggta
     2281 tggctactag atgggccaat cataactggt ccgatgatat caaagacaga ttgaaaacga
     2341 agtgatttca tcagtttccc cttataaaaa ttcttatctc tattatatca aagaatgagg
     2401 ggcttggctt taattgtgga tgaaaacctt tctaatacct caaatagcat aaaaatagta
     2461 tcttttatga caaaaaacac cttatttagg gaaataaaaa ataattttgt aatatttcta
     2521 cataaaagtg tcaagaaacg gtaatattta aagggtatga tagaactata gaaagaagga
     2581 gaattttcga atatgaaatc aataactaaa aagattaaag caactcttgc aggagtagct
     2641 gccttgtttg cagtatttgc tccatcattt gtatctgctc aagaatcatc aacttacact
     2701 gttaaagaag gtgatacact ttcagaaatc gctgaaactc acaacacaac agttgaaaaa
     2761 ttggcagaaa acaaccacat tgataacatt catttgattt atgttgatca agagttggtt
     2821 atcgatggcc ctgtagcgcc tgttgcaaca ccagcgccag ctacttatgc ggcaccagcc
     2881 gctcaagatg aaactgtttc agctccagta gtaagtgaaa cagttgtttc aactgtaagc
     2941 ggatctgaag cagaagccaa agaatggatc gctcaaaaag aatcaggtgg tagctataca
     3001 gctacaaatg gacgttatat cggacgttac caattaacag attcatacct gaacggtgac
     3061 tactcagctg aaaaccaaga acgtgtagca gatgcctacg ttgcaggacg ttacggttca
     3121 tggactgctg ctaaaaactt ctggcttaac aatggctggt attaagaact aacgaacaaa
     3181 taatatgtaa agagtcgagg aaatccctcg acttttttta taatttcttt ggttgataat
     3241 gggtctctac ttcttttttg agatgagaca gcatagaagt ttcctcggtc agctctagga
     3301 gtgagaaccc tttttggata agtttcgcat catccctaca aatcccaata cggacatagc
     3361 agatagccag cctatcgtag cttttcttat tcttggcgtc ctccagtaaa gtgtagcaga
     3421 tttgcttaca catgttttta tcttgattgt atagatagag tgtggaaagg tttaatagaa
     3481 ttgtcattcg caagtcatat aaatgttgat agtttttata gacttctaag cgttgcaaga
     3541 ttttctcgga ttattgacaa ataaacggac attctgatag acgattgccc tagcggctta
     3601 gtgtaaactt tacaaatttg gcttgtaatt tttttataaa attcacttca gttacttgac
     3661 agttattata ttttcgctta tgcttattat atagataaaa ggacgggata ggagtttaaa
     3721 aatttgaaag cgctatccat aatcataacg cataattttc tccttttcgt gaaaaaagtt
     3781 aacgtaattt tgaaatgagc ttaataagag gtactatgta ggtagaaaat aaagatagga
     3841 gaaaaagtat gaataaaaaa atattttgta ttctagtatg tattatatca ttgattagtc
     3901 tagctataat ctttccatgg ggctggtcga tataatgtaa gggagcttga gttgttagtg
     3961 gaggataaaa tgttaagagt aatagtagtt attcaatctt tagtaataat actgcttgct
     4021 gttgctttat tattatctcg gaaaaacttt gattcgttaa atgttgttct tataatgatg
     4081 atcgtatcac aattgttgtt attagtatat ttgataaaat cagggaatca gtgattagct
     4141 gttatttgaa ttaagtaaac tgttaagctt caagcagtaa ctggttttaa atatttaaaa
     4201 taagataaag aaaggaagtc taatttaggt aactaaacaa agcctgcgat cacttgttag
     4261 gaaaccatcc taaccatgct taataaaaaa ggagaaaata aatggaactt gtattaccaa
     4321 ataattatgt tgtgattgat gaagaagaga tgatgtacct tgatggggga gcttatttaa
     4381 gcaagcgtgc ttgtcaagga atttgcgcag ctttagctat gagtccagga acttttatag
     4441 cattagctgg agctgcagtt ttaaccaaaa aactaataaa ctatattaaa gttggaggcc
     4501 ttggaggttg gcttattggt gcagcagcag gtgtattggc tggggcggca ggaagaatag
     4561 cttactgtat tggatatggt gctcttaata gaggttgtga tattagcggg aacccttatc
     4621 cttgggatgg attcatatct gcgacagtaa gatgagtaat gtagataaga ttcgaaaaat
     4681 aca
//
LOCUS       DAVMMR010000093         4652 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMMR010000093 DAVMMR010000000
VERSION     DAVMMR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.093"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            66..230
                     /locus_tag="VYS74_001933"
     CDS             66..230
                     /locus_tag="VYS74_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV3789920.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            309..1037
                     /gene="yaaA"
                     /locus_tag="VYS74_001934"
     CDS             309..1037
                     /gene="yaaA"
                     /locus_tag="VYS74_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV3789921.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1088..2572)
                     /locus_tag="VYS74_001935"
     CDS             complement(1088..2572)
                     /locus_tag="VYS74_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3789922.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2788..>4652
                     /locus_tag="VYS74_001936"
     CDS             2788..>4652
                     /locus_tag="VYS74_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3789923.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGVAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKENVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKGEKGDRG"
BASE COUNT         1578 a          757 c          949 g         1368 t
ORIGIN      
        1 aaaaaggttt atttaataaa tatatgttat aatagagtaa attaagacta atggaggaca
       61 aagaaatggg tttttatttg atggttgctt caatgctact cggactgctc gctttgaaga
      121 taggtttttc tcagttcaag gagaaaaaag ataaattctt atctatctta acaagtttag
      181 ctggcacagc ccttgttctc gtggctgtct ggttaggatg gcccaaataa atagaaaaac
      241 ctcggtcaaa ccgaggtttt tcaaatgaat ttcttggttg tggctaaaaa atatgctaca
      301 ctatcaatat gaaaatttta atcccaacag caaaagaaat gaacacagac ttgccaagta
      361 tcgaggcaat tcctttaaaa ccagaaagtc agaccgtgct tgatgccttg gctctctatt
      421 ctgccagtca attggagagt ttctacaagg tatcagctga gaaagcggcg gaagaatttc
      481 aaaatatcca agctttgaaa aggcaaactg ctcaacacta tccagccttg aaactttttg
      541 atgggcttat gtaccgcaac attaagagag ataagctgac cgaggcggaa caagattatc
      601 ttgaaaatca tgtcttcatt acctcggctt tgtacggtgt tgttccagcc ttgtcaccca
      661 tggctcctca ccgtttggat tttttgatga aattaaaagt cgctggtaag actttgaaga
      721 gccattggaa ggcagtctat gatgaagctc tgaagaagga agaagtgatt ttctctctct
      781 tgtcatcaga gtttgagact gtattttcta aggaaatcag agcaaagatg gtgaccttca
      841 aattcatgga ggatagaggc ggtcagctga agattcactc aactatctcc aagaaagcgc
      901 gcggggcctt tctaacagct ttaatagaaa atcaagtaca aactgtgggg gaagcacgtc
      961 gcttgaactt tgctggattt gtttaccgag aagatttgtc acaaccacag ggattggttt
     1021 ttgtaaagga agtatagaaa tcagataaga gaaaagtcag cacgattatg ctgacttttt
     1081 tgataactta taaagtattg gacaaatgaa tctcatttga taaggtgtta agatgattaa
     1141 ccaaattcat gattgacaga tgatttcggc aaataaactt cttggtaatc cctggaatat
     1201 agaaattaga cacgatgata tcgtaatctg tctgatttaa aatctcagga cttgttttta
     1261 attcatccca aatatcgaag gtaaagcggt tattacagta ataggaaagc atatcaacaa
     1321 aggtaaggga tatagcatgg tcaaagttac tgataatcaa aaccttgata ggtggtcgat
     1381 tttctaacaa ctgagtagag aggttttcag catgggttaa gatggtgtac atcaagtgtt
     1441 ccaactgttc aggatggtca tgctgtccaa ttgcctgacg gtattgagcc agttcttgac
     1501 gcgcacttcc cataaagtct gggaagtaaa cttcaaattt tttaatcgtc aatgcttttt
     1561 gttcaaataa gattggggta gaaaagattt cttgtctttc aaagaaagca gtgttgtgta
     1621 aatgccaaat cagctcatcg tggttagtaa aagtaatctt atattgtttt gataggcgtt
     1681 ctaatatttg actaagtaat tggtaagagt atcttgcttg actgttatct tctaaagagt
     1741 tgaagaattc ttgaggatct aggaaaatat cattctgaag aaaagaaata aaaatttgag
     1801 cgattgtatc tggagtcatt tcaagcccaa aatgtttaga gaaataagca agtgtctcct
     1861 ggaaatttgg aatgctcttt aaaagaggat aatattcttt gtaaaagtgg ttggggaggt
     1921 caatgaagtg accattttta accctgtgga tactaatagc tatcatcagt ttgtacattc
     1981 tataaattga aaatcgcatt ggataattcg tgattttata gaagaagtca gcaaactctg
     2041 ttaaatcttc ctcaggaagg tcaggaaaag gccaatctag gaaataatag cgctcagaaa
     2101 agtattgggc aaaaaagtaa cgaatatcga tctcatttcc acgtatctcg gatggagtaa
     2161 aagataattc aatttgaaac tgagatgaaa gaacctttgt aatatttctg cccagtcgat
     2221 agaggttggc actactaaag taaagatttt ctattgttcg ataaataggt agcccctcat
     2281 tgaagaacat atactctagt aattgaaaag attgagaatt agcaagaaaa tattgataaa
     2341 tatcttctac actagtgtta acttctaaat ctatcatgat tccgttaaca gaagattgga
     2401 tgttaataga tggaaaggca atacgtaatt catttaaatc actttttaag attcgttctg
     2461 tacatcctaa tttttcggct agttcatgta atttcatcca attacgattc tgaattaggg
     2521 tttccattaa gcgtaattgt ctttgaactt ttgtggataa aaggtttctc atacgggtat
     2581 ttcctccata attcattata tctttttttg tctaagtagt ataaaaaaat tagagcaaga
     2641 aaattgtcgt tagtaatcgg aaaaattaga ataatataag tggcttttaa gtgcagaaac
     2701 agagtatatt aattgttcag aaaatattga ttaatgcctc aaatagttgt acattgtaaa
     2761 ctataaaata ctatctagga gtttaaaatg aaacattcac ataaaaaatc atttgactgg
     2821 tatagcatgc aacaacgtta ttctattcgt aagtattact ttggtgcagc tagtgtcttg
     2881 ctcggtaccg ctttggtatt aggtgtagca gctagtgtcc aaacagtaca agcggaagaa
     2941 aacaaacaag aaactaccaa tagtatttct gttggtaggg gagaagcagc tactaaacca
     3001 gcagaggttt ctgcgtctaa taaagagaaa acctatgcag ctccaactgt agctaatcca
     3061 gtagaaacga ctccagttaa aactgaagaa gttactaaac cagcagaaaa agttgaagaa
     3121 gcaaaagaca aaaaagagga agtaacgcat caagatgccg ttgacaagtc aaaattatta
     3181 acggctcttt cgcgtgctaa aaaattagaa agcaagttat atacagaagc aagtgctgct
     3241 aacttgcaaa caagtatcca agctggtcaa agcttgcttg gaaaagcaga tgcaactgaa
     3301 gctgaattat cagcagcaga gtcatctatt caatcattta ttattggtct agaacttcgt
     3361 tctaactcta ataaagaaac tgtatcagaa acgcctgtag cgaagaaagc tgatgcagtt
     3421 gaatcaaaag aaggggctaa accagctgca acaactgaac gttcagctgt tgatagcgct
     3481 attttgccaa ctagcacagc tgacaaagta gaaacaactt cagctccagc atctattaat
     3541 gaaatcttga aactaggttt gagcctttct gatgctcgcc aaaatccagc tatccgtaag
     3601 gaaaatgtta atagagggta tagtggtttt agagcggcta gcaatccagc caacccaatt
     3661 gtctcaggtt ctggaaatac agttgcattt gcagatatta gccaaggtgg tcgtagctat
     3721 agtttccgtg gctacgggaa ctcacgtggt ggaaattcta ttcattacga tgtaacaaca
     3781 gtacgtagtg gtaatagtgt taactttaca attagttatt ctgcgccagg agattctaga
     3841 gagtttgtta ataataattt tatcttggat aaaggggatg gatttggaaa tccttcaaat
     3901 gcaacgatca caagttcaaa tccaagagta agggagcaat caaaatctat tagtcagggt
     3961 gccaactacg tatctcactc aggatacagt atgacctctg ctatttcaac aaatacggaa
     4021 caaactatca gatttagttt gcctatcatt aatctaaatg gtgatttgtc tgttcgtttg
     4081 aaacctgtta cttttaacgt ggaccaaggt ggtggtggtg ccgctactag caatgaccca
     4141 tacagtaact ctaactatta ttatagagca aacccactat acttggatgc aaacccttat
     4201 ggtggtacta ataataagac tgtttcagaa gacattgact tccaaactgt ctatcttcca
     4261 actagtaagt taccagaagg tcaaactaga ttagttcgag aaggtgaaaa aggacaacgt
     4321 caaattacct ataaagttca tcgatttggt aacgaaacac ttttaggatt gccgattagt
     4381 aatagtgtta ctaaagaagc taagccacgt attatgcaaa ttggtgtggc taaagatcta
     4441 atcgatacag taaaaccacg tgttgatcaa aataaagtcg gtgatacaaa taacctcact
     4501 ttctatcttg ataacgatgg aaacggtgtt tatactgaag gtgtagacga acttgttcaa
     4561 aaaattgcta ttaaagatgg agctaaaggt gaaaaaggag accaaggtga acgcggtcta
     4621 actggagcta aaggtgaaaa aggagaccga gg
//
LOCUS       DAVMMR010000094         4606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMMR010000094 DAVMMR010000000
VERSION     DAVMMR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.094"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..309
                     /locus_tag="VYS74_001937"
     CDS             <1..309
                     /locus_tag="VYS74_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3789924.1"
                     /translation="GAMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMA
                     TGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV
                     "
     gene            710..1831
                     /gene="mnmA"
                     /locus_tag="VYS74_001938"
     CDS             710..1831
                     /gene="mnmA"
                     /locus_tag="VYS74_001938"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV3789925.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1972..2427
                     /locus_tag="VYS74_001939"
     CDS             1972..2427
                     /locus_tag="VYS74_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3789926.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2437..4350
                     /gene="mnmG"
                     /locus_tag="VYS74_001940"
     CDS             2437..4350
                     /gene="mnmG"
                     /locus_tag="VYS74_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV3789927.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMILPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1334 a          937 c         1128 g         1207 t
ORIGIN      
        1 ggcgctatgg caacaggttg ggctaaagtc aatggttcat ggtactacct caacgctaat
       61 ggtgctatgg caacaggttg ggctaaagtc aacggttcat ggtactacct caacgctaac
      121 ggttcaatgg caacaggttg ggtgaaagat ggagatacct ggtactatct tgaagcatca
      181 ggtgctatga aagcaagcca atggttcaaa gtatcagata aatggtacta tgtcaatggt
      241 ttaggtgccc ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa
      301 tgggtttaag ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac
      361 aaagataagg ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat
      421 ttcaggaaat gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga
      481 aaatgggttg ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac
      541 atcattcaca taatctgtat attgactata agttttaaaa aacaattttt aagctcttcc
      601 ttgtcttctc taaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa
      661 gcacacacga ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa
      721 ctctaaaaca cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct
      781 tttgctcaag gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac
      841 agatgaaaac ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga
      901 ccagattggc attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt
      961 tgagtatttc ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa
     1021 caaggaaatc aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt
     1081 agcgactggg cactatgctc gagtggcgcg tgatgaggat ggcactgttc acatgcttcg
     1141 tggcgtggac aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact
     1201 ccaaaaaacc atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga
     1261 agaagcaggc ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga
     1321 aaagaacttt aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac
     1381 tgtggatggt cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg
     1441 tggcggactc ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg
     1501 aaaagatcta agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat
     1561 gtcaactagc ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac
     1621 gctagaatgt acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt
     1681 caaaggagac aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca
     1741 ggcagttgtc ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta
     1801 ccgcgatgga caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat
     1861 aataacagca atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc
     1921 acttctttgt agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa
     1981 caagactttc ggacaaaagt agacaatacg gtttttggtg ttcgggcgac agctttgatt
     2041 cttcaaaatc gcaagcttct agtcaccaaa gacaagggca agtattacat tatcggcggt
     2101 gcgattcaag ttaacgaaaa aacggaagac gcggtagtcc gtgaagtgaa ggaagaactg
     2161 ggtgttaaat ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac
     2221 ggcgtttcct atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg
     2281 ttgaccatgc aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctt
     2341 gaggatatcc agctagttcc agccttttta aaaacggccc taccagattg ggaaggccaa
     2401 ctaagacaca ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa
     2461 tacgatatta ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc
     2521 cgtatgggct gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct
     2581 tgtaatccct ctatcggtgg ttctgccaag gggattgtcg tgcgtgaagt cgatgccctc
     2641 ggtggcgaga tggccaaaac cattgacaag acttacatcc agatgaagat gctaaacaca
     2701 gggaaggggc cagctgtccg tgcccttcgt gcgcaggctg acaaggaact ttactctaag
     2761 gagatgcgca agacggttga aaaccaagaa aatctgaccc ttcgtcaaac catgattgat
     2821 gagattttgg tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat
     2881 gctgctaagg ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga
     2941 gacctcaagt actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat
     3001 ctcaaggaac tgggcctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag
     3061 gcttcttcta tcaattacga tgtgacagaa atccaaccag gagatgaagc acctaatcac
     3121 ttctcataca cttcacgtga tgaggattat gtcaaggacc aagtgccatg ctggttgacc
     3181 tacaccaatg gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt
     3241 acaggtgtgg tcaagggagt gggacctcgt tactgtccat cgattgagga caagattgtg
     3301 cgctttgcgg acaaggaacg tcaccaactc ttccttgagc cagaaggacg caatacagag
     3361 gaggtctatg tccaaggact ttcaaccagt ctgcctgagg atgtccaacg tgacttggtt
     3421 cattccatca aaggtctgga aaatgcagag atgatgcgaa caggttatgc tattgagtat
     3481 gacatgatct tgcctcacca gttgcgtgcg actttggaaa ccaagaaaat ctcaggactt
     3541 ttcactgctg gtcagacaaa tggaacatca ggttacgaag aggcagcagg ccaagggatt
     3601 atcgcaggga tcaatgcggc tctgaaaatc caagataagc ctgagttgat tttgaagcgt
     3661 agtgacggtt acattggggt catgattgat gatttggtga ccaagggaac catcgaaccc
     3721 taccgtctct tgaccagtcg tgctgaatac cgtctcattc tccgtcatga caatgctgat
     3781 atgcgcttga ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt
     3841 tttgaaatca agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc
     3901 aagccagtca aggaaaccaa tgctaaggtt gaggagatgg gcttcaaacc cttgacagat
     3961 gcagtgacag ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtagcc
     4021 ttcatcggac cagctgcaga ggatttggat gataagatta tcgaattgat tgaaacagaa
     4081 atcaagtacg aaggctatat ttcaaaagcc atggatcagg ttgccaagat gaaacgtatg
     4141 gaagaaaaac gcattccagc caatattgac tgggatgaca tcgattctat tgcgacggaa
     4201 gctcgtcaga agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg
     4261 ggggtaaacc cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt
     4321 atttctaaaa ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca
     4381 aagcttggct ttgattcatc tccagcctcc catagtttcc cgaactatgg gagctaactc
     4441 aagttatgca ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa
     4501 agcatagaga aaaacagctc cgaagacggg gctgttttgt tgacttgtac tcaatgaaaa
     4561 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactg
//
LOCUS       DAVMMR010000095         4600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMMR010000095 DAVMMR010000000
VERSION     DAVMMR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.095"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            8..454
                     /locus_tag="VYS74_001941"
                     /pseudo
     CDS             8..454
                     /locus_tag="VYS74_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(447..614)
                     /locus_tag="VYS74_001942"
     CDS             complement(447..614)
                     /locus_tag="VYS74_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV3789928.1"
                     /translation="MKKRILSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(779..2386)
                     /locus_tag="VYS74_001943"
     CDS             complement(779..2386)
                     /locus_tag="VYS74_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV3789929.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPAIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2539..4023
                     /locus_tag="VYS74_001944"
     CDS             2539..4023
                     /locus_tag="VYS74_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV3789930.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4118..4600
                     /locus_tag="VYS74_001945"
     CDS             4118..4600
                     /locus_tag="VYS74_001945"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV3789931.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1349 a         1023 c          884 g         1344 t
ORIGIN      
        1 tgtttagatt gatgaaacag gattcgatac ttatttttat cgagaatatg gtcgctcatt
       61 aaaaggtcag ttaagaagag gcaaagtatc tggaagaaga tatcagagga tttctttggt
      121 tgcaggtcta acaaatggtg agttaatcgt tccaatgact tacgaagaga cgatgacgag
      181 cgactttttt gaagcttggt ttcagaagtt tttcttacca acattaacca caccatcggt
      241 tattattatg gataatgcaa gattccatag aatggggaag ctagaacttt tatgcgaaga
      301 gtttgggcat aaacttttac ctcttcctcc ctactcacct gagtacaatc ctattgagaa
      361 aacatgggct catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta
      421 cgaggctctt ttgtctttct cttgtttcaa ttgactatat tatcagcgta atagtctaat
      481 agatagttaa atgtagttgg atttccacta aaggttgatg agtaagtttt tgtatctgaa
      541 cctgattggt cgcaagcagc taaaagcaaa gcagatgcaa aagtcaggcc tgcactaagg
      601 atacgcttct ttatgttcgt cttctttctc cttaatagtg ggaatttgta aagttaattg
      661 aatttcgaga atgaaagttt ataaaccttg gttataaaaa acaaaggatt tccgcctttt
      721 atacagtcct cccttgtttt tatatgattt caattttaaa tttttctgca aaaaatattt
      781 atagtaattc cacacagaaa gcatcccatg gaactaagat ttgtttttca aagacttctt
      841 gagctagggt gttttcaatc aagacagatt tgacttttcc ttctactgtc aagtcttgct
      901 cttcattgga caagttagcc acaactagga agcgacggtc gccatcctta cgtatataag
      961 caaagacctt atcagccgta tcaagcaatt caaagtcagc tcgaattagc caactattct
     1021 ccttgcgaat ttggaccagt ttctgatagg tatagaaaat agaatctgga tttgccagcg
     1081 cttcttggac gttgatcatc tcgtaatttg gattaactgc caaccaaggt tgatctgttg
     1141 agaaaccagc gtttttgctc tcgtcccatt gcataggggt acgggcattg tcacgtccaa
     1201 taacacggat actgtccatg atttcttcaa tcggaacacc tttttcaaga gcctcacgcg
     1261 catagttgag agattcaata tcttctactt gatccagtgt ttcaaacgga tagttggtca
     1321 tcccaatctc ctcaccttgg tagatataag gagttcctct cataagatga agcaagattg
     1381 caaaggcttt ggcagatttt tcgcggtatt cttggtcatt tccccagatt gagacgatac
     1441 gagggaggtc atgattgttc cagaagaggg aattccaacc gtcctcaact cctaactctg
     1501 tctgccattt gttgaagatt tcttttaact tagcgatatt cagctctttt tgatagtgcc
     1561 atttaggctg accttcctga tactgaagac cgatatgttc aaactggaag accatagaca
     1621 attcttgccc ctttggatca gagtaaagct tggcaatcgc tggcgttgct ccccaagtct
     1681 cccctactgt caagagatcc ttatctccaa aggtcgcctg attcatttcc ttgagatagg
     1741 gatggagcat aggaccatta ttgactacct tctcgtcagg aattttgcca atcatgtcaa
     1801 taacatccat acggaaacca ccaatacctt tatcaatcca gaagttcatc atctcataaa
     1861 ttttctggcg aagtttttca ttttcccagt tgagatccgg ctgtttcttg ctgaaaaagt
     1921 ggagatagta ttgacctgac ttttcatcgt attcccaagc agacccacta aagatagaat
     1981 ctaggtcatt gggttcatcg cgccagatat agtagtctcg ctcagggctg tcagtatttt
     2041 cacaggcttc gacaaaccaa gcatgttcat ctgaggtatg attaaccacc aagtccatga
     2101 tgatacgaat gtcacgcttc ttagcttctg caatcagctg atccatgtcc tccatggttc
     2161 caaaaatagc cgcaatcgct tgataatcag caatatcata gccattatca tccatagggc
     2221 tgtcataaac gggagaaagc caaattgctg tgattcctag cttggctaga tagtccaact
     2281 tactggtaat acctggcaaa tcaccaactc catctccatt actatccata aaactctttg
     2341 gatagacttg atagactacg gcattgtgcc accatttttc ttgcatctgt ttacctcatt
     2401 attttaataa tctatagtat atgatagcac tttttaaaat tttgtgcaag cgtttgctta
     2461 atctttttaa tttatttttt agctccgtag tttcctaact tccaattttt tattataata
     2521 gagtccagag gtaaaaaaat gaaaaaacaa gcttttagtt ctgaacagta tttgaattta
     2581 caacgcgacc acatcttaga gcgcattaac caatttgacg gcaagcttta cctagagttt
     2641 ggtggcaaaa tgttagaaga tttccacgct gctcgtgtcc ttcctggtta tgaacctgac
     2701 aacaaaatca agctcttgca agaattgaaa gagcaggttg aggttgtgat tgccattaat
     2761 gctagcaaca ttgaacattc caaagcacgt ggcgacttgg gcatttctta tgaccaagaa
     2821 gttcttcgtt tgattgacaa attcaatgaa ttagggattt ttgttggttc cgttgtcatt
     2881 acacaatacg ctggccaacc cgctgcagat gccttccgca accaacttga gaaaaacgga
     2941 attgattctt atcttcatta tccaatcaaa ggatatccga cggatatgga tcacatcatt
     3001 tccccagaag gcatgggcaa aaacgactac atcaaaacca gtcgcaactt gattgtcgta
     3061 accgctcctg gacccggttc tggaaaattg gcaacgtgta tgtccaatat gtaccacgac
     3121 caaatcaatg gcatcaaatc tggctacgct aaatttgaaa ccttccctgt ttggaatctt
     3181 ccccttcatc atccagttaa cttggcttat gaagctgcca cagctgacct tgatgatgtc
     3241 aacatgattg accccttcca tcttcaaacc tatggagaaa ccactgtcaa ctacaaccgt
     3301 gatatcgaaa tcttcccagt gctcaagcgc atgttggaac gtattctcgg aaaatcacca
     3361 tacgcttcac cgacagatat gggtgtcaac atggttggtt tcgctattac agatgacgag
     3421 gctgctgtcg aagcttctaa acaagaaatc atccgccgtt actatcaaac agttcttgac
     3481 ttcaaagctg aaaaagttgg cgaagctgcc gtcaagaaaa ttgagttgct catgaacgac
     3541 ctcggtatca cacctgcaga ccgtaaggtt gctgtcgttg cgcgccaaaa agcagaagaa
     3601 actggtggac cagccctagc ctttgaattg ccaaatgggg aaatcatcac tggtaagaac
     3661 tcagaactct ttggccctac agccgctgcc ttgatcaatg ccatcaaaaa atcagctgac
     3721 atcgctaaag aagtaaaact aatcgagcct gaagttgtta agccaatcca aggtcttaaa
     3781 atcgatcatc tcggtagccg caatccacgc cttcattcaa atgaaatcct gattgcactt
     3841 gctatcacag ctacagaaaa tcctgatgct gcccgcgcta tggaagaact cggcaacctc
     3901 aaaggaagcg aagcccactc aaccatcatc ttgactgatg aagacaagaa tgtccttcgt
     3961 aaactgggta tcaacgtaac ctttgaccca tactaccaat acgaccgctt atatcgtaag
     4021 taaagatttc aacctctcca gcctcggaga ggttttctct ctgtctcagg agcaaccttt
     4081 atgatataat gagagaagaa aactgaaagg atttaccatg tcaaaagaag ttattgtcga
     4141 aagttttgaa cttgaccaca ccattgtcaa agcaccctat gttcgcttga ttggggaaga
     4201 aacaggacca aaaggagaca tcatctccaa ttatgatatt cgcttggtgc aaccaaacga
     4261 agactctatc cctactgccg gccttcacac tatcgagcac ctcttagcca aactcatccg
     4321 tacccgcatt gacggcatga ttgactgttc accatttggt tgccgcacag gcttccacat
     4381 gattatgtgg ggacgtcaca ccagtgctaa aatcgcagct gttatcaagg attcgctcaa
     4441 ggaaatcgct gaaactacta cttgggaaga tgtcccaggg acaaccatcg aatcttgcgg
     4501 aaactacaag gaccacagcc tcttctctgc taaagaatgg gcaaaactca tcttggaaca
     4561 agggatttca gatgatgcct ttgaacgtca tgtgatttaa
//
LOCUS       DAVMMR010000096         4514 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMMR010000096 DAVMMR010000000
VERSION     DAVMMR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.096"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..191
                     /locus_tag="VYS74_001946"
     CDS             <1..191
                     /locus_tag="VYS74_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789932.1"
                     /translation="KPLTVEDKTYNHALSKERSKVENIFAKVETFKMFSTTYRNHRKR
                     FGLRMNLIAGIINHELGF"
     gene            complement(180..827)
                     /locus_tag="VYS74_001947"
                     /pseudo
     CDS             complement(180..827)
                     /locus_tag="VYS74_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(936..1454)
                     /locus_tag="VYS74_001948"
     CDS             complement(936..1454)
                     /locus_tag="VYS74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV3789933.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1681..2550)
                     /gene="rsmI"
                     /locus_tag="VYS74_001949"
     CDS             complement(1681..2550)
                     /gene="rsmI"
                     /locus_tag="VYS74_001949"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV3789934.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2553..2870)
                     /gene="yabA"
                     /locus_tag="VYS74_001950"
     CDS             complement(2553..2870)
                     /gene="yabA"
                     /locus_tag="VYS74_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV3789935.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2910..3800)
                     /locus_tag="VYS74_001951"
     CDS             complement(2910..3800)
                     /locus_tag="VYS74_001951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV3789936.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3797..4435)
                     /locus_tag="VYS74_001952"
     CDS             complement(3797..4435)
                     /locus_tag="VYS74_001952"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV3789937.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1267 a         1017 c          726 g         1504 t
ORIGIN      
        1 tcaagccgct aacagttgaa gataaaacct ataaccatgc gctatctaag gagagaagca
       61 aggttgagaa catctttgcc aaagtagaaa cgtttaaaat gttttcaaca acctatcgaa
      121 atcatcgtaa acgattcgga ttacgaatga atttgattgc tggtattatc aatcatgaac
      181 taggattcta gttttgcagg aagtctaatg taattctcaa gctgtgagtt cattatgatt
      241 tgtaaatcct atatatcttc agcagctaaa aacacatttt gaaatgttcc aagtccgttt
      301 tcaccaaaac tatattctct cttactatac ttactcttat tattctctat atagttagag
      361 tcaaggttct taacctcttg aagttcattt tctttacttc tgaggttaaa atcttttact
      421 tctgaagtta agttttttga cttctgtaag tcattttctt ttacctgaaa tatactcata
      481 aagtctttaa cataaatgat gttcggctta ccaagtccaa gccttactct ttcgatcagt
      541 cctattcctt tttttatatc aagctcatct aatgttttta tggcagttgg ctttgagata
      601 tttcttcttt tcataatttc ttcgacagta aaatagataa atactctgcc ttccttgtct
      661 atccagttat tcttaaatga cattcctgtc tatttaagaa gtatggaata aaggataatt
      721 gcttctgctg ataagccccg aaattcttct ctatcaacta atatctccgg tacttttaag
      781 aagttaaatc ttttcgcttc tctgttataa aaatattcaa agtccatgca agatcacctc
      841 ccttcttaaa aatttgcaaa gaaaaagacg atagtttttc ttctatcgcc tttggtaacc
      901 atttttatga aattttttaa agttaaaaat ataatttata atactatctt cttccatttt
      961 ctgattttag ttctgaaagt tccaaaaggt gcaaccgtat ttacatgaat aaacttatac
     1021 acttcccaag tcgctgtttt agtcgcttca tcagcccact ttctcatatg cggttcaaat
     1081 aattcttcct cactcaacga atcaatcatt gcagagatag aatgaatatt ttcatttaat
     1141 tttgctttca actcttgcag agataaatga gcgtaggtat ctgtgaacca ctgatataat
     1201 tcaccaagtt gattccattt aaatttatcc gatggtgttt ttacttgaag cccctttctt
     1261 tcatcttctt cccatttaag aaccaagttg gtccaaccaa cctgataagc aaggttttct
     1321 gctggagttc tgtcaacttc atcagctctc ttatctttta aattttctgg aatattatca
     1381 aattctaaaa tatatttctc aaatgttttc tctatctcag cttttagttc ttctttattt
     1441 tcgtatgttc gcaaaaaatc accttctttc tgatttatca atcctttgat ttaaaatgac
     1501 cttatttttc tatttatacc ttctaagaac gctataactg ctgctccaac catcggtatt
     1561 ccgtatgggc ttaataaaaa acttaatacc aaagcttcta taccaagtga tacatcttta
     1621 tattatcatc actaattaag ttaaacagaa acagacagaa ttattacatc ctgtctccct
     1681 ttattgtttt tcttcccagt cgtggtaggc agcgtagagt tgactcttat tccactggta
     1741 aatcttagct acttccttaa tagcttgatt tttcttcata ccttgctgaa tacgagcttg
     1801 gatttctaag aacaagtctt cctcgccttt ttcttccaca tcctgactgg caccttcaac
     1861 aatcagaaga cattcaccct tgagagacgt ttcagcaatg ctttccagca attcagaaat
     1921 tgtacctctt tggtattctt catagatttt ggtcaattcc ctgaccaaga caacggagcg
     1981 gtcaccgtag acttctagca tattttccaa tgtatctgcc acacgatggg gcgattcata
     2041 aaaaatctga gtttcaggat agtctttttt cgagtcgaaa aattgctttt gctggcctga
     2101 ttttctcggt aaaaagccgt aaaagatatg tggctgcggc gctaaaccac tggcaatcaa
     2161 ggcggaaatt cctgcagagg cacctggaac tgtaacaact gcaatttctt cctcaatagc
     2221 tgccttaacc aaatcatgac cagggtctga aatgctaggc aaaccggcat cagagacctg
     2281 agcaatactt tgccctgctt tcaagaaacc aatcaaatca ggaatttttt ccttggcatt
     2341 gtgctcatga aaactgatct gcttggtgga aatgtcaaaa tgcttgagca aaagccctgt
     2401 attgcgcgta tcctcagcag caatccagtc cacttctttc aaggtctgga tagcacgaaa
     2461 agtcatatca tctagattgc caatcggcgt tgccactaga tacagcttgc catagggaga
     2521 ctgcccctta aaactttttt gaatttgcat gcttactccc tgtataacaa ctcatcacag
     2581 aacatacatt cttcgtcctg ctctcgacgt tgtccataaa aatcattaca tacgtgaaat
     2641 ccatcacggt aaatacgacg gacactttcg cgaacatgct tggccttgac gggagcgtct
     2701 gcttccacct cacccaagcg ttctcgcaac ttactatttt ccaagcgaag agctgtattt
     2761 tcttctacca agctcttgag atttttcttg atggcttcca catcggctaa ggttaccaat
     2821 aattgttggg aaaaatcatc cagcgcgtca aataattctt ttttgtccat aaaacagccc
     2881 tttcctttct ttatcattca tttttgagtt tatatttctt tcaagaccag atattccatg
     2941 gcattttgaa agctgacatt agcttgccac atttttctag cttctagtaa atcttgtaga
     3001 atcactctta ctcttacctg caagaggtcc tgcccacaga gaacttcaag aatccgtaaa
     3061 acctgatcct gtttttcctt atcatctgcc aagttggcta atttggcaac ctgtagataa
     3121 ctttcttttt tcttagctac taaccaagtc agcaggcgtt cactttcatc gaccaaggtc
     3181 caaaaacttg cctgattagc caacttttct gcttcagctc gcgattgact aaacttagct
     3241 aaaagagtcg cttttttctt aacaagtccc atttgttcta agagtaagat aagtttttct
     3301 tcttgctttt taaagtggaa gatctgagtc cgacttcgga ttgtcggtaa catcttttcc
     3361 tcatcgctag tcaagaagaa aatataaact tcactctggg gttcttcgat gaccttgagc
     3421 agagaattgg ctgcgttggg atgcatttta tccgcttgct cgataataaa gacctgttgc
     3481 tggctttcaa tccctgcttg agaaaactgt cccaccaatt cccgaatgcg ttctgtcttg
     3541 atgacctgat tgactggctt aatcaaggtg acatctggaa actcttcctg ttcaatcagc
     3601 ttgcaacttc ggcatttctc acatggtaag acgccaactt tatccgtaca aaagaggctc
     3661 ttagctaaaa attgcgccat ttccaagctt ccaaagaaac ctgaaaagag ataggcgtga
     3721 ttgagctggt cttgttctaa gatacggaca aaacggtcaa actgagctgg ttgccaagcc
     3781 tttagttgat cttgtttcat ttggccaagc ccattccgtc aaacaagaca gccttggtag
     3841 tttccacaac ttgctccaaa gggagactag catcaatctt gacaatgcga tttccctctt
     3901 tatccagaag agaaaggtag ccttgacgaa cttttttatg caagtccaac ccttccaaat
     3961 ccaaacgatt aacctcgcgg tcactattcg cagcaatacg agccagccct tcttccacct
     4021 cgatgtcaaa atagagtgtc aaatcgggtt tgaggccatc tgtcgcaaac tgattgagcc
     4081 agtcaatggc ttcaatatct aagccacgac caaatccctg ataggcaaca gaactatcga
     4141 taaaacgatc catgatgacc aacttgccag cttcaagggc tggaagaact ttttccacca
     4201 aatgctgtct gcgactggca atatagagaa gtagctctgt tttagcatcc atctgagtat
     4261 gacttggatc caaaatcact tcccgaatct tctccccaat caagactccg ccaggttcac
     4321 gggtcgtcaa cacctctact cctttttcct ttaaaattgg tagcagagcc tctaaaacac
     4381 tggtcttgcc tgctccctct ggtccctcaa gagagactaa aaatcctttt gacatgtcta
     4441 actcatttct tttttactac ttctattcta tcaaaaaaat aggggtttgt gacaatcttt
     4501 ttgtcttctc attt
//
LOCUS       DAVMMR010000097         4411 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMMR010000097 DAVMMR010000000
VERSION     DAVMMR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.097"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            94..738
                     /locus_tag="VYS74_001953"
     CDS             94..738
                     /locus_tag="VYS74_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV3789938.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(780..2768)
                     /locus_tag="VYS74_001954"
     CDS             complement(780..2768)
                     /locus_tag="VYS74_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3789939.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2770..3528)
                     /locus_tag="VYS74_001955"
     CDS             complement(2770..3528)
                     /locus_tag="VYS74_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3789940.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGIFYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3956..4147
                     /locus_tag="VYS74_001956"
     CDS             3956..4147
                     /locus_tag="VYS74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789941.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1347 a          870 c          856 g         1338 t
ORIGIN      
        1 ccagagaaga cttctggatg gctaacttga acttgaaatt tagcgtcaga aacaaatcac
       61 gaaattgatt caaattttgc aggtcgttta aatatcctgc gtgcgggtgt tcttgatgct
      121 aacgatggaa ttatttccat tgctggtgtg gttatcggag ttgccagtgc cacgaccaat
      181 atctggatta tctttttatc aggttttacg gctatcttag ctggtgcctt ttcaatggct
      241 ggtggagaat atgtatccgt ttcaactcca aaagataccg aggaagctgc cgtttcgcga
      301 gaaaaactct tgctagacca agatagggaa ctagccaaaa aatccctcta tgctgcttat
      361 atccaaaatg gagaattcaa aacttctgcc caactcttga ccaataagat ctttcttaaa
      421 aatccactca aggctctggt agaggaaaaa tatgggattg agtatgaaga atttaccaat
      481 ccttggcacg ctgccatttc tagcttcgtt gcctttttcc ttagaagttt gcctccaatg
      541 ctgtcagtga ccatttttcc aagtgattac cgcatccctg ctaccgtcct tattgtcggt
      601 gtggcccttc ttctcactgg ttacacaagt gctagacttg gaaaagcccc aaccaaaaca
      661 gctatgattc ggaaccttgc tattggtctc ttgaccatgg gagttacctt cctgctcgaa
      721 caacttttca gcatttagaa tacaagaaat acctcgattt tgaagtcgag gtatcttttt
      781 tacatttgca caatcttgcg ataacttctt gaagtaatca tgaaaatcag cacataggcg
      841 atgaggaaga tagcgcagat agacaaggtc acaatcaaca tcatagtcgt atccagtaca
      901 ccaatcactt ttaaaatcag gctaagcata tggtaggcaa aggcgagatg tatgaaggca
      961 aagagcaaag gaaggaagaa aacagttaaa acctgtttgt tgatggtttg cttgatttgc
     1021 ttttggtcca aaccgacttt ctgcaagata ataaagcgtt cacggtcttc gtagccttca
     1081 gaaatttgtt tgtagtagat gaccagaaca gttccgacca taaagataat ggataggaaa
     1141 ataccgataa agaagacacc accaaagagg gcactcatct gagaactagc atctgctaga
     1201 ttgctaccat aaacatagct accttctgtg tctaattgag cattaaattg attgaggtag
     1261 ttttcatact cctcagcgac cttgagttgt tcttcttcgc tggcatctac attcatacca
     1321 ccgtaaaact gattatagat agccgaatct gggaattgat ccaaaaaggc ttgtaaatca
     1381 ggaacaacaa ggtaattgta atcagtagtc aagatattaa acttatttgg aacatggttg
     1441 acaataaaat ctttagtaaa ttcttctttg acagaaaatt gatgatcatt tagagttaga
     1501 gctttctgtc ctttcagtcc gtcatttttg gcaaagagac cgacctcatt tcctgataga
     1561 gacagttttt gaccagtcat attttcataa tctttttggt caaataccat gaaaactgtt
     1621 tttggttgga cacggttttg tcctttttca aaaatagtta acttggttcc ttcttgattc
     1681 gcaacagcaa agtaagtgta acgaagcact tctttctctt taatcttata acctttgtca
     1741 cttgcaaact ggctcaagag tttgtccaaa tcttcttttt caacattttg ccctgaaacc
     1801 ccaaaatcat gaggatttag aactttttta aagctttctg aggaattgaa aatgcttgtc
     1861 gctgctgaca tggttaccaa aaccattgtt gacaagatag cgatggtggc tagtccaact
     1921 gcatttttct tcatacggaa aatcaagtta gaaacagata tgagattatt tggttggtaa
     1981 tagtatttct tatttttctt taagatttgg aggaaaacgg taatccctgc attaaacaag
     2041 agataagtcc caaagataac cagtaaaaca gctatgaaga aggttgttaa ggctgtaaga
     2101 ggatctttta ccgtaagggc aagataatag ccaattccta aacttatgga accaagaatg
     2161 gtttggagag gaaggaagcg accttttttc tctccactag ctttctcacg agagagctgg
     2221 agggcattca tacgggcgat tcgaagggca ttcaggaaca tgaggcctag gaaaatcaaa
     2281 ccgaagacaa caagcactgt aatgacaact ttcgtctgga aggtagcaac cagctcaacc
     2341 ttcaatttca ttagtttgag caggaaagcg aaaattaact tgtcaaacaa ggctccaata
     2401 ccgatacccg ctccaacagt tagaatccca aataccacta actccttaaa ggtcatactg
     2461 ataagatgac gcttctccaa gcccaacatg ccataaattc ctagttcctt ggaacggttc
     2521 ttcatgacaa aactattggc atagagaacg ataatggctg acgcaagggt gacgacaaac
     2581 ataccaaatc caagtgtagc ctgaatggtt gttcctccac ggatttccgc aatcttagga
     2641 ttgaaggtta gagagtaaaa gagataggtg acagtgactg ccaagagaac agccagcgca
     2701 aaaggatagt agagtttgcg gtttttaatc aagttcgata ccgctaactt attggttaat
     2761 cgaaacatac taattcacct cgcttgccat gacagtcaag gtatcagaga tttcttggaa
     2821 catctgacgt tctgtcttct ctccacggta gatttgattg taaaaaatgc cgtctttgat
     2881 aaagagtaca cgtttagctc tgctagctgc tgctgttgag tgggttacca tgagaatggt
     2941 ttggccgcgc tcattgattt catcaaagac atcaagtaag gctgcagatg acttggagtc
     3001 aagagctcct gtcggttcgt ccgcaaggag aatttcaggt tcagtgatga tggcgcgtgc
     3061 tactgctaca cgctgtttct ggccaccaga aatctcgtaa gggtacttct cttgtaattg
     3121 gtttattccc agattctcag ctgtcaccac caatttcttc atcatctctg taataggtct
     3181 tcttgacaag acaagcggaa gcaagatatt gtccttaaca gacagagtat ctagcaagtt
     3241 aaagtcttgg aagacaaatc ctagcttttc acgccggaaa ctagaagcct gtgaattttt
     3301 aatagttgct gtatcagttc cgtttaggta aacctgccca cgacttggtt tatccagcat
     3361 agctaggata ttgagcagag tggatttacc agaaccagac tcacccatga tagcaacgta
     3421 gtcacccttt tctacggtga agtgaatatc cttgagggcc tctacttggt tgccctgaaa
     3481 acgagtttta taaatttttt gaacgtgttt tacatctaaa agtgtcatga gaatctcctt
     3541 tcttaatatc ccatcaaatg aaatgtagct tgcatcatag tgcatcccag ctgaatgcgc
     3601 tcaatttctt tgtgacttgg ttttctagtt ttcttatgta agatttgttt catgatgtta
     3661 ctcctttgtt ctttatgagt ctagtttaca tcaaaaaaag accgcttgcc ataacctaac
     3721 cttacaaaag agactttaat cttacatttt tgtaagattg ggtgaaatat aagcaaaatt
     3781 acttagaaaa tgatttgatt tcctattttt aaagttgtaa aatataagta tgaaaaggct
     3841 tactttaaaa aggaggaagc gggaactaac attttagtaa ggttacctag ataacatgcc
     3901 attcaattta acacttgcaa gtcgttcaac ctataaggaa agaaggtgcg ctttaatgaa
     3961 acataaagaa catatactca tcggatttct ctatctgctc tctccattca tcggtcagct
     4021 cttagttgaa cacacacact ttatcagtac agaatttacc ggcactgctt atgttatttg
     4081 ctggttatca gttgttatca gcatccacca tttttccaaa aatgtattgt ctcaacagca
     4141 aaaatagatt aaaaaactgc gtttaaatta ctgcttttat agtaaaatga aataagaaca
     4201 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat tttttagaag tataggtgtc
     4261 ctattctagt tccaatccac tatagtttgt ggatgaagct ggcgaaatat tattaatact
     4321 attcaaaaat caaagtgcaa actatatcaa cttcacattg ccgactaaat tacagcctgc
     4381 ggctggcttc ctagtttgca ctttgatttt t
//
LOCUS       DAVMMR010000098         4244 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMMR010000098 DAVMMR010000000
VERSION     DAVMMR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.098"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            409..636
                     /locus_tag="VYS74_001957"
     CDS             409..636
                     /locus_tag="VYS74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789942.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            924..3869
                     /locus_tag="VYS74_001958"
     CDS             924..3869
                     /locus_tag="VYS74_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV3789943.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGQQFYGNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESG
                     RNGKPNKDGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DKVVISNLRDNPEAKDPAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTP
                     EKAYEKIGDKTYQILYKQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGN
                     LYKGQELIGNIYFAHSTKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKF
                     LGTGPGTGIVLRNGPHKGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGL
                     FTLGKKREQ"
BASE COUNT         1451 a          675 c          926 g         1192 t
ORIGIN      
        1 ttatctacaa cctcaaaaca gtgttttgag ctgacttcgt cagtcttatc tacaacctca
       61 aaacagtgtt ttgagcatca tgcggctagc ttcttagttt gctctttgat tttcattgag
      121 tataaaaaca gatgagtttc tgttttcttt ttatggacta taaatgttca gctgaaacta
      181 ctttcaagga cattattata taaaagaatt ttttgaaact aaaatctact atattacact
      241 atattgaaag cgttttaaaa atgaggtata ataaatttat taacacttat aaaaagtgat
      301 agaatctatc tttatgtata tttaaagata gattgctgta aaaatagtag tagctatgcg
      361 aaataacaga tagagagaag ggattgaagc ttagaaaagg ggaataatat gatatttaag
      421 gcattcaaga caaaaaagca gagaaaaaga caagttgaac tacttttgac agtttttttc
      481 gacagttttc tgattgattt atttcttcac ttatttggga ttgtcccctt taagctggat
      541 aagattctga ttgtgagctt gattatattt cccattattt ctacaagtat ttatgcttat
      601 gaaaagctat ttgaaaaagt gttcgataag gattgagcag gaagtatggt gtaaatagca
      661 taagctgatg tccatcattt gcttataaag agatatttta gtttaattgc agtggtgtcc
      721 tggtagataa actagattgg caggagtctg attggagaaa ggagagggga aaattggcac
      781 caatttgaga tagtttgttt agttcatttt tgtcatttaa atgaactgta gtaaaagaaa
      841 gttaataaaa gacaaactaa gtgcattttc tggagtaaat gtcttatttc agaaatcggg
      901 atatagatat agagaggatc agtatgaatc ggagtgttca agaacgtaag tgtcgttata
      961 gcattaggaa actatcggta ggagcggttt ctatgattgt aggagcagtg gtatttggaa
     1021 cgtctcctgt tttagctcaa gaaggggcaa gtgagcaacc tctggcaaat gaaactcaac
     1081 tttcgggggg gagctcaacc ctaactgata cagaaaagag ccagccttct gaggaaggag
     1141 aaaatataac tcttcctgca gagcatgtag agtctgtttc agagactgaa ctttctggca
     1201 ataagcaaga acaagaaagg aaagataagc aagaagaaaa aattccaaga gattactatg
     1261 cacgagattt ggaaaatgtc gaaacagtga tagaaaaaga agatgttgaa accaatgctt
     1321 caaatggtca gagagttgat ttatcaagtg aactagataa actaaagaaa cttgaaaacg
     1381 caacagttca catggagttt aagccagatg ccaaggcccc agcattctat aatctctttt
     1441 ctgtgtcaag tgctactaaa aaagatgagt acttcactat ggcagtttac aataatactg
     1501 ctactctaga ggggcgtggt tcggatgggc aacagtttta cggtaattac aacgatgcac
     1561 ccttaaaagt taaaccaggt cagtggaatt ctgtgacttt cacagttgaa aaaccgacag
     1621 cagaactacc taaaggccga gtgcgcctct acgtaaacgg ggtattatct cgaacaagtc
     1681 tgaaatctgg caatttcatt aaagatatgc cagatgtaac gcatgtgcaa atcggagcaa
     1741 ccaagcgtgc caacaatacg gtttgggggt caaatctaca gattcggaat ctcactgtgt
     1801 ataatcgtgc tttaacacca gaagaggtac aaaaacgtag tcaacttttt aaacgctcag
     1861 atttagaaaa aaaactacct gaaggagcgg ttttaacaga gaaaacggac atattcgaaa
     1921 gcgggcgtaa cggtaaacca aataaagatg gaatcaagag ttatcgtatt ccagcacttc
     1981 tcaagacaga taaaggaact ttgatcgcag gtgcagatga acgccgtctc cattcgagtg
     2041 actggggtga tatcggtatg gtcatcagac gtagtgaaga taatggaaag acatggggag
     2101 ataaggtggt tatctccaat cttcgagata atcctgaagc taaagatcct gctgcgccat
     2161 cgcctctaaa tattgatatg gttttggttc aagacccgac aacaaagaga atcttctcaa
     2221 tttatgatat gttcccagaa ggtcgagcag tttttggaat gccaaaaaca cctgaaaaag
     2281 cttatgaaaa gataggggat aaaacttatc aaatcttgta taaacaagga gagtctggtc
     2341 attatactgt tcgtgagaat ggagaagtgt ataatgcaca aaatcaaaag acggattatc
     2401 gtgttgtagt gaatccaaca gaacctggct atagagataa aggaaatctt tacaaaggtc
     2461 aggaattgat tggaaatatc tattttgcac acagtacaaa aaatccattt agagtagcca
     2521 atacgagcta tctatggatg tcatatagtg acgatgatgg taaaacttgg tctgcaccga
     2581 gagatattac tccaggtctt cgcaaggatt ggatgaagtt cctaggaaca ggtcctggaa
     2641 caggaattgt acttcggaat gggcctcaca agggacggat tttgataccg gtttatacga
     2701 ctaataatgt atctcactta gatggctcgc aatcttctcg tgtcatctat tcagatgatc
     2761 atggaaaaac ttggcatgct ggagaagcgg tcaacgataa ccgtcaggta gacggtcaaa
     2821 agatccactc ttctacgatg aacaatagac gtgcgcaaaa tacagaatca acggtggtac
     2881 aactaaacaa tggagatgtt aaactcttta tgcgtggttt gactggagat cttcaggttg
     2941 ctacaagtaa agacggagga gtgacttggg agaaggatat caaacgttat ccacaggtta
     3001 aagatgtcta tgttcaaatg tctgctatcc atacgatgca cgaaggaaaa gaatacatta
     3061 tcctcagcaa tgcaggtgga ccgaaacgtg aaaatgggat ggtccacttg gcacgtgtcg
     3121 aagaaaatgg tgagttgact tggctcaaac acaatccaat tcaaaaagga gagtttgcct
     3181 ataattcgct ccaagaatta ggaaatgggg agtatggtat cttgtatgaa catactgaaa
     3241 aaggacaaaa tgcctatacc ctatcattta gaaaatttaa ttgggacttt ttgagcaaaa
     3301 atctgatttc tcctaccgaa gcgaaagtga agcgaactag agagatgggc aaaggagaga
     3361 tgggcaaagg agttattggc ttggagttcg actcagaagt attggtcaac aaggctccaa
     3421 cccttcaatt ggcaaatggt aaaacagcga ctttcctaac ccagtatgat agcaagacct
     3481 tgttgtttgc agtagataag gaagatatcg gacaggaaat tattggtata gctaaaggaa
     3541 gcatcgaaag tatgcataat cttcctgtaa atctagcagg tgccagagtt cctggcggag
     3601 taaatggtag caaagcagcg gtgcatgaag ttccagaatt tacaggggga gttaatggta
     3661 tagagccagc tgttcatgaa atcgcagagt ataagggatc tgattcgctt gtaactctta
     3721 ctacaaaaga agattatact tacaaagctc ctcttgctca gcaggcactt cctgaaacag
     3781 gaaacaagga gagtgacctc ctagcttcac taggactaac agctttcttc cttggtctgt
     3841 ttacgctagg gaaaaagaga gaacaataag agaagaattc taaacatttg attttgtaaa
     3901 aatagaagga gatagcaggt ttttaagcct gctatctttt tttgatgaca ttcaggctga
     3961 tacgaaatca taagaggtct gaaactactt tcagagtagt ctgttctata aaatatagta
     4021 gattgaaata agatgtgaac aactctatca ggaaagtcaa attaatttat agaaaaattt
     4081 tagcagtcaa ggtgtactgt tatagattca atatattata cgactattaa ccttatcttc
     4141 tcctaaaatt gactttcttg ttttcttatc ttgtctactc gaaacaagta ttgtaagaat
     4201 ttgattattt ttgaaagtac ttttaatata cttgatatag ttaa
//
LOCUS       DAVMMR010000099         4203 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVMMR010000099 DAVMMR010000000
VERSION     DAVMMR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.099"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(231..491)
                     /locus_tag="VYS74_001959"
     CDS             complement(231..491)
                     /locus_tag="VYS74_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789944.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(550..1101)
                     /locus_tag="VYS74_001960"
     CDS             complement(550..1101)
                     /locus_tag="VYS74_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789945.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1091..2086)
                     /locus_tag="VYS74_001961"
     CDS             complement(1091..2086)
                     /locus_tag="VYS74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789946.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(2494..2919)
                     /locus_tag="VYS74_001962"
     CDS             complement(2494..2919)
                     /locus_tag="VYS74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789947.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3063..3431)
                     /locus_tag="VYS74_001963"
     CDS             complement(3063..3431)
                     /locus_tag="VYS74_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789948.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(3453..3659)
                     /locus_tag="VYS74_001964"
     CDS             complement(3453..3659)
                     /locus_tag="VYS74_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789949.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(3646..3807)
                     /locus_tag="VYS74_001965"
     CDS             complement(3646..3807)
                     /locus_tag="VYS74_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789950.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         1366 a          825 c          556 g         1456 t
ORIGIN      
        1 ttttttcatt tcactatact aaaaaaccac ctgaaacaaa tcaagtgact agaggataaa
       61 aacaaagact gggcaaaaag ttttgaaaag caaaaaacgc ataatatcag gtcttgaaaa
      121 accttgacgt ttgattgtgg gaagattgac ttcattttct cctgaaattg aagttttttc
      181 tagtttccag atatagacga attcaatata gccatctcgc tacaattatt ttaaccatcg
      241 agaaaatttt tcagaatttc aaaaatttca ttttgatcca atgaaatgtc atcattttga
      301 agaatagttg acttcacagg ctctaattct atcacaaagc ggtgtatgtc tgcttttacc
      361 gcactacttg atgttatagc cgataacttt tcatctccta gcaaggaagc tgtcaaacgg
      421 gtaatgtcat tcaaatgctt tttaatggac ttacttaaac cttgagcacc tgtagcagag
      481 cgttccctca tatccagcca agctttagcc ttaaaaataa ttaatactca atgaaaatca
      541 aagagcaaac taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg
      601 gaagctgacg tggtttgaag agattttcga agagtataaa ccacaattac tcaataccga
      661 atacccctga atggtttctt tttcatgcac caatatatta taataatctt catccaataa
      721 taaggctgat aaactagcat cttggtcaaa atgtaaggga atttctcgac cgtccttctt
      781 taatggatat tctggtaaga tactaaatag ttcaatcata gaaggaaact caggattagt
      841 tgttgtaaat cgaaaaagct gcgctttctc atctttctga cttccttgat actctcccaa
      901 ttctaaaaaa tgattcaagg tagtataaaa ttccttattt tttacttcat aaatgatgac
      961 catatcataa tcttttgttg tgcgactttt aaatccttgc gaatccaata cgatagaggt
     1021 agcagttccc ccaatcagaa cataatagtt ctgaaaatcc gcaaacattt cttgaaaaat
     1081 aactttcgta ttagctggca tcatcttctc ccagatactg taatatcata ttttctagtg
     1141 cttcactctc ttcctctata cgtgggtcat catcatcttt taaggtcaaa taaagagaaa
     1201 tcggatctac aaattgttta tcatgattat ttttaaaatc attccaaaac tcagatacaa
     1261 aaggacgata tttccatatc tctagcatct ttccttttaa aacatgctga gaaagtggca
     1321 aggataactg attgaatttt ctctgccata tgacatagct agtattttca tccgtttcag
     1381 ctaaaaaagt tgaatgcgac aaagcataag caccaccata tagaaggtta gaaacagatt
     1441 ttatctgctt tatatcgcca tctggcaata aaatccgttt tttgatggga ttaaataaac
     1501 atgacacgga ttttaagaat aattctttct ttgacaccgt atatgtgtaa agcttatttt
     1561 gcttgtttaa ccaatataaa gctttaaaag tcctcaaaca cctataaatt gttgagtttg
     1621 gaagtccagt gacttgtgaa agcaaatcaa catctactac tttttgacct tttgtcaata
     1681 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggact tcagtatcat
     1741 tcgcattcag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
     1801 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
     1861 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
     1921 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
     1981 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
     2041 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
     2101 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
     2161 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
     2221 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
     2281 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
     2341 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
     2401 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
     2461 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
     2521 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
     2581 gttattactt cctaatatcc cattatgctt tttcgctaag gaaatagaag ccacttctcc
     2641 cttaccaata atcttgttac tatcatgatt tcttgttaaa tctctatata atgcgtattc
     2701 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
     2761 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
     2821 aacaatttta cccgaataga gcttttctaa aagatgttca gtaccaaccc ataaaaatac
     2881 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc cccctcttca
     2941 tctagcccat atacaatatc atatctgaaa gcatctagta acagttcctc atacttccct
     3001 tgcgaaattc tgttattttc taaaagttgt tcagttgaat taacatatta tcctaatatc
     3061 atttactttt tactttctga caaaggacga tataaacttg tatcatagcc taatcttgaa
     3121 gctgtctcta taacactaat atccatattt ttaatttctt ctgcatcaag gtatccatca
     3181 ttcctcaatc tatataacat aactttatga ctgataccat aaaactgacc caattttata
     3241 atatcttcta cttcaagatg agttctattg gcattttctc taatttcctc aaccatccta
     3301 tacagtgaag atgggaaaat taaaaaataa gaagcaaact gatccgcttt tctttcagtt
     3361 tcatctcctt caccaatcaa gataagactg actgaactct tcttcacctc atcataataa
     3421 agatgataca gttcatgtgc taaagaaaat ctttaccttc ctaatgacat gtctgaattg
     3481 actgcaatga gactgaactg agttccttta taacagactc cgctaatatt ctttccgagt
     3541 ccataaaata ccagcgtcaa attttctatc ttttgtacca atttaaaaat atctatcggc
     3601 gattcaccat cagctcccaa cttttttcta agatttgaag ctttattact taaatccatt
     3661 cgtgatattc ctttcacctt tttccatttt ggcaatcatg ctttgattca aagacaaata
     3721 ctcagcaatc ttatcttgag taagatttga agatattcta agttctttta ttctttttcc
     3781 aacatcacat acatttatca catacattta tcatattcat acacctctca taaaaagaat
     3841 agcacactct tgtcataatt ttttaaataa aaaaattatg acaaaacaag gaagcaattt
     3901 attgatgctg cttaaaaatc taaaattgat gagttcctat caaaaatcgt atcttcaacc
     3961 tcaaaacagt acttaaagct atccgactcg gtttacattg tcaaatttag attttatttg
     4021 agcataactt tctagtttgc tttttgattt ttgtttaata tagtagcaaa attgaaggaa
     4081 atatctccac aagaaagcgc atactattaa gctttttcaa gacctaataa tatgcgctgt
     4141 tctgatttga aagacattcc attattattt tactgtaatc aagccatctg gctctactgt
     4201 gaa
//
LOCUS       DAVMMR010000100         3904 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVMMR010000100 DAVMMR010000000
VERSION     DAVMMR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.100"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            25..1086
                     /gene="dinB"
                     /locus_tag="VYS74_001966"
     CDS             25..1086
                     /gene="dinB"
                     /locus_tag="VYS74_001966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV3789951.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSN
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1233..2078)
                     /locus_tag="VYS74_001967"
     CDS             complement(1233..2078)
                     /locus_tag="VYS74_001967"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV3789952.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2334..3193
                     /locus_tag="VYS74_001968"
                     /pseudo
     CDS             2334..3193
                     /locus_tag="VYS74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3494..>3904)
                     /locus_tag="VYS74_001969"
     CDS             complement(3494..>3904)
                     /locus_tag="VYS74_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV3789953.1"
                     /translation="WKLRLDNRDGVLNEAGAEVYYLWTSFENMLREIARLDKAELESI
                     VVWNRLLVYATLFGYADKVSHLMKVHQIQVENPDINLYVAYGWHSMFYHSSAQMSHYA
                     SVANTASTYSVSSGSGSSGGGFSGGGGGGSIGAF"
BASE COUNT         1253 a          823 c          745 g         1083 t
ORIGIN      
        1 gaaaagtata aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga
       61 aaaatcatcc atattgacat ggatgccttt tttgctgcag tggaaatcag ggataatcct
      121 aaactcagag gaaaacctgt cattattgga agcaaccctc ggcaaacagg tggacgggga
      181 gtcgtttcta cctgtagtta tgaggcaaga gcttttggtg tccattctgc catgagttcc
      241 aaggaagctt atgaacgttg tccccaggct gtctttatct cagggaatta tgagaaatac
      301 aagtctgtgg gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc
      361 atgagtattg acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg
      421 gtcaaaattg ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttct
      481 gccggtattt cctataacaa gttcttagct aaaatggcga gtgattatca aaaacctcat
      541 ggtttgacag tgattctacc tgaacaggct gaggattttc tcaaacaaat ggatatttcc
      601 aaatttcatg gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact
      661 ggtgctgatt tacttgaagt tcctgaggtg accctaatag accgttttgg tagactaggc
      721 tatgatctgt atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc
      781 cgtaaatcaa tcggcaagga gaaaacctac gggaagattc tccgtgctga ggaagatatc
      841 aaaaaagagc tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa
      901 gctggaaaaa ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga
      961 aaaagtcttg ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc
     1021 tatgaagaat taagtgagaa agaaagaggt gtccgcctat tggggattac catgactgga
     1081 ttttaaagct caatgaaaat caaagagcaa actaggaggc tagccgcagg ctgctcaaaa
     1141 cactgttttg aggttgcaga taaagctgac gcggtttgaa gagattttct aagagtataa
     1201 aaccttgaag agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa
     1261 agtagcaaaa cactaccaag cacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt
     1321 ttcacatagc tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc
     1381 gcgaccaaga gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg
     1441 aaaatcttta aggcactagc tccaaacata acaggaatcc caagatagaa ggtaaattct
     1501 gtcacaactg aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg
     1561 ctagtccctg gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa
     1621 ggaagcttgt ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc
     1681 aaatagatga aggcaatccc gtagataatc aacatgagag caactgaaac catgttatgg
     1741 aagtgggtat caaaccaatc atcaaattta aagacaccaa gtaaaggtaa agtagcaatc
     1801 aagaccttca accatagtct ccaagtctta cgaacttcct gtttgtcctt agtcggttta
     1861 aaaggattga gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc
     1921 acgacattaa acatggacat aaaggcttca ttttgatttt ggtattggat aaactcctct
     1981 gctaaaatca agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatc
     2041 ccgaagaaga tagattttaa aatttcaata agatacatat atttctcctt atattatatt
     2101 taacgcagtt agctactgcc aaagcccaag tggtatactt ggaataagcc actgtggatt
     2161 agttcatttc ctccaatcgc ttgacgattt ccgaaagggt ctaaccacag ctttttgttt
     2221 caattggtaa tgagtttgat aacgcataga cgttttatct cgtctaaggt aacaaggcaa
     2281 aaagttcgtg gataaccaag tagacttgaa atactatatc tggaggtact cccatgcttt
     2341 atgtgggcat tgatgtcgct aaaaataaac acgatgttac agccttgaat gttcgaggaa
     2401 aactgttctt aaaccactca ctttttcaaa taataaagct ggttttgaac tcttagatct
     2461 gtcttttcga cagctcaacc aagactgtct catcgctctt aaacttcttt ctgaccccaa
     2521 tcgtgaacaa tttcaacacg ataatcggca agtagaacta aaaatactgg ctagacatat
     2581 tcatcgtctc aagaaaaaac agtctgattg gaaagtacaa tacactcgtt gtcttgatat
     2641 catctttcct gagttggata aaatcgttgg aaagcattca gaatatacct accaactctt
     2701 gacacgctac cctaatcctc agaaaaggct tgaggcagga tttgataagc tgatagaaat
     2761 taagcgattg accgcttcta aaattcagga tatcctctca gtcgcacctc gttctatcgg
     2821 aacaacatct cctgctcgtg aattcgaaat catcgaacat tacaagaggc tcattgacaa
     2881 ggcggaaaca tgtgtcaatg acttgatggc tgagttcaac tcggtcatca cgacggttac
     2941 tgggattggg ggtcgtttag gggcggtcat tttagccgag attcgaaata ttcatgcctt
     3001 tgataatcct gctcaattac aagctttcgc tggactggat tcttctattt atcagtcagg
     3061 tcagattgat ttagctggaa gaatgatcaa acggggttcc cctcatctgc ggtgggcact
     3121 catacaagct gccaaagcat gcgctcgctt ttcacctgct tttaaggcct atcttaagac
     3181 taagttagaa taaggaaaac attacaatgt agccatcatc caccttgcaa aaaaacttat
     3241 ccgaaccctg ttttatatcc taaaaaagag ctgccatttg acgaacaaaa agtgatgtaa
     3301 tttctgaaat taacacagga ttacctttga aaaaatggac tttttcaagg gctttttgcc
     3361 gtgctctaaa atcatcgcca aatcatcaaa ttgaaataag aagtagttta gataaaacta
     3421 ttgacttttc ttatagtttg tctttcttct atcttccatt atatcatact ttttcagtct
     3481 atggtagctc tctttaaaag gctccgatac tgccgccacc tccgcctcca gagaagccac
     3541 cgccagaact tccacttcca gaagatacgg agtaggtact tgctgtattt gcgacactag
     3601 cataatggct catttgcgcg cttgaatgat aaaacatact gtgccagcca taagctacat
     3661 agagattgat atctggattt tcaacttgaa tctgatgaac cttcatcaaa tgactaacct
     3721 tgtccgcata gccaaataag gttgcataga ccaagagacg attccaaaca acaatacttt
     3781 ccaattcagc cttatccagt cgtgcaatct cacgtaacat attttcaaaa ctggtccaga
     3841 gatagtagac ctcagctccc gcttcattta gaacaccatc acgattatct agtcgaagct
     3901 tcca
//
LOCUS       DAVMMR010000101         3662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMMR010000101 DAVMMR010000000
VERSION     DAVMMR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.101"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..497
                     /locus_tag="VYS74_001970"
     rRNA            <1..497
                     /locus_tag="VYS74_001970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            549..621
                     /locus_tag="VYS74_001971"
     tRNA            549..621
                     /locus_tag="VYS74_001971"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Ala,seq:tgc)
     gene            746..3647
                     /locus_tag="VYS74_001972"
     rRNA            746..3647
                     /locus_tag="VYS74_001972"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1027 a          753 c         1073 g          809 t
ORIGIN      
        1 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg
       61 agcgcaaccc ctattgttag ttgccatcat ttagttgggc actctagcga gactgccggt
      121 aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta
      181 cacacgtgct acaatggctg gtacaacgag tcgcaagccg gtgacggcaa gctaatctct
      241 taaagccagt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt cggaatcgct
      301 agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg
      361 tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg agccagccgc
      421 ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg
      481 gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt agtcttgaga
      541 ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag gaggtcagcg
      601 gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat tgaaaattga
      661 atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat taataagagt
      721 ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt ggatgccttg
      781 gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag ctgtaagtaa
      841 gcgatgatcc agggatttcc gaatggggga acccaacagg taatacctgt tacctacatc
      901 tgttaaggat gtgaggagga agacgcagtg aactgaaaca tctaagtagc tgcaggaaga
      961 gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg gcagaagggc aaaccgaaga
     1021 gtttactctt cggggttgta ggactgcaat gtggactcaa agattataga agaatgattt
     1081 gggaagatca gccaaagaga gtaatagcct cgtatttaaa atagtctttg tacttagcag
     1141 tatcctgagt acggcgggac acgtgaaatc ccgtcggaat ctgggaggac catctcccaa
     1201 ccctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca
     1261 ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt tcgagcccgt
     1321 taatgggtga gagcgtgcct tttgtagaat gaaccggcga gttacgttat gatgcgaggt
     1381 taagttgaag agacggagcc gtagggaaac cgagtctgaa tagggcgcct tagtatcatg
     1441 acgtagaccc gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg taagacgcac
     1501 tggaggaccg aaccagggca cgttgaaaag tgcttggatg acttgtgggt agcggagaaa
     1561 ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc tagcgtcgac
     1621 attagagatt cttggaggta gagcactgtt tgggtgaggg gtccatcccg gattaccaat
     1681 ctcagataaa ctccgaatgc caatgaatta tggtcggcag tcagactgcg agtgctaaga
     1741 tccgtagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat aattgttaag
     1801 tggaaaagga tgtggggttg cacagacaac taggatgtta gcttagaagc agctattcat
     1861 tcaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg taccggggct
     1921 aaaacaattt accgaagctg tggatacctt tataggtatg gtaggagagc gttctatgtg
     1981 tgatgaaggt ataccgtgag gagtgctgga acgcatagaa gtgagaatgc cggtatgagt
     2041 agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg ggaaggctcg
     2101 tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga tggacaacag
     2161 gttgatattc ctgtactaga gtatgtagtg atggagggac gcagtaggct aactaaagca
     2221 gacgattgga agagtctgtc taagcagtga ggtgtgaatt gagtcaaatg cttaattcta
     2281 taacattgag ctgtgatggg gagcgaagtt tagtagcgaa gttagtgacg tcacactgcc
     2341 aagaaaagct tctagcgttt aaacatactc tacccgtacc gcaaaccgac acaggtagtc
     2401 gaggcgagta gcctcaggtg agcgagagaa ctctcgttaa ggaactcggc aaaatgaccc
     2461 cgtaacttcg ggagaagggg tgctgactta aagtcagccg cagtgaatag gcccaagcaa
     2521 ctgtttatca aaaacacagc tctctgctaa atcgtaagat gatgtatagg gggtgacgcc
     2581 tgcccggtgc tggaaggtta agaggagtgc ttagcgtaag cgaaggtatg aattgaagcc
     2641 ccagtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     2701 gttccgaccc gcacgaaagg cgtaatgatt tgggcactgt ctcaacgaga gactcggtga
     2761 aattttagta cctgtgaaga tgcaggttac ccgcgacagg acggaaagac cccatggagc
     2821 tttactgcag tttgatattg agtgtctgta ccacatgtac aggataggta ggagtctaag
     2881 agatcgggac gccagtttcg aaggagacgc tgttgggata ctacccttgt gttatggcca
     2941 ctctaaccca gataggtgat ccctatcgga gacagtgtct gacgggcagt ttgactgggg
     3001 cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc cctcagaatg gttggaaatc
     3061 attcgcagag tgtaaaggta taagggagct tgactgcgag agctacaact cgagcaggga
     3121 cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag ggccatcgct caacggataa
     3181 aagctaccct ggggataaca ggcttatctc ccccaagagt tcacatcgac ggggaggttt
     3241 ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt cggtcccaag ggttgggctg
     3301 ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt cgtgagacag ttcggtccct
     3361 atccgtcgcg ggcgtaggaa atttgagagg atctgctcct agtacgagag gaccagagtg
     3421 gacttaccgc tggtgtacca gttgtcttgc caaaggcatc gctgggtagc tatgtaggga
     3481 agggataaac gctgaaagca tctaagtgtg aaacccacct caagatgaga tttcccatga
     3541 ttatatatca gtaagagccc tgagagatga tcaggtagat aggttagaag tggaagtgtg
     3601 gcgacacatg tagcggacta atactaatag ctcgaggact tatccaaagt aactgagaat
     3661 at
//
LOCUS       DAVMMR010000102         3626 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMMR010000102 DAVMMR010000000
VERSION     DAVMMR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.102"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1295)
                     /locus_tag="VYS74_001973"
     CDS             complement(<1..1295)
                     /locus_tag="VYS74_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEV3789954.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSN
                     GYSIYNTGTSIQTMLTLGSGLGTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGN
                     GAQMNGWQAKREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASA"
     gene            complement(1293..1406)
                     /locus_tag="VYS74_001974"
                     /pseudo
     CDS             complement(1293..1406)
                     /locus_tag="VYS74_001974"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1652..2113)
                     /locus_tag="VYS74_001975"
     CDS             complement(1652..2113)
                     /locus_tag="VYS74_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3789955.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2080..2223)
                     /locus_tag="VYS74_001976"
     CDS             complement(2080..2223)
                     /locus_tag="VYS74_001976"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV3789956.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            2492..2872
                     /locus_tag="VYS74_001977"
     CDS             2492..2872
                     /locus_tag="VYS74_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3789957.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            2971..3192
                     /locus_tag="VYS74_001978"
     CDS             2971..3192
                     /locus_tag="VYS74_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3789958.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3209..3523
                     /gene="trxA"
                     /locus_tag="VYS74_001979"
     CDS             3209..3523
                     /gene="trxA"
                     /locus_tag="VYS74_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3789959.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         1087 a          733 c          683 g         1123 t
ORIGIN      
        1 cttgctgaag ctgaggcgct tgtgcttgct gaggctgagg cactggttga tgctgaggct
       61 gaggcgcttt gactagcact tactgagagt gacttagact gtgaaagtga ctgactggtc
      121 gtgctagatt caactacctt tcctctacca ttgaagtagt tagttctaat gttatctgtt
      181 ttagcagcgt aaggggtaaa tgtaaaggat gtatccgttc cagtaattgg tacagtccaa
      241 gatgatgtta atccatattc tctcttagcc tgccaaccat tcatttgggc accatttccc
      301 caagtatacc tactaccccg cttcgtcatt gtagatgtat tatagtattg aacctgtcca
      361 ccatttttgt tagtaatgga gttctttaca tctgaagggg taccaagacc actgccaagg
      421 gttaacattg tttgtattga agttcctgta ttatagatag aatatccatt actcatcttt
      481 tttgatatat taccaagagt ctttgtttta ggatccacat acgtaaccgt ataggtaaag
      541 gtcagtttta agccatcatt tgtcactgtc aatcgatagt aaattggaac attatatacc
      601 gaatctacac ctttacctgt ataaactttt gtctcacttt tagcaatgtt tagacttgca
      661 ttaattgcag gggcgccatt tacaatcgta ttgccagaaa aaacagcagc ttttatagaa
      721 gccagcaatt gctcgatgga atccactgaa cgcttgcgtc gcttagcata agattggaga
      781 ttgacatttg taactgatgc tacataatca ctagctggtt tcttacgatc ttcttcgacc
      841 ttcttagcag ttgcttctgt agcggcagct gtttgtgatc ctaccacagt agatgatgca
      901 gagatacttg tcgaagccga tgtacttgct gattctgatg cacttgtact cgctgaggtc
      961 gaagcgctgg ttgaggctga ctcagatgca gatgtacttg ccgactctga agctgacaaa
     1021 ctagttgaac tcgctgaatt acttgtagat atcgtaccta gaactactgt accatttgtt
     1081 gccaaagcat ccgttttctc tacagttttt tcaagtactg ccgactcatt tgtaaatacc
     1141 ttcgtttgag ttgcaacggt tccacttatg acagttcccg cagcaactat ccccttgagg
     1201 atatcaagcc cagtaattga atgacttact ttatcttcta ccgtttcggt catgacctga
     1261 gtagtatcaa caccaccacg catgacctta aacaagccaa aaagagaagt cgaggcccgt
     1321 agccaatgct tccctgattt gattaatttg aaccgagtta ctcggtctgt ttctctatat
     1381 tcacccttct gacgtctaaa aaatattttg aaatttcctt tcgagtatta ttttcatgta
     1441 tggatataaa taaattacca tactataatt atagaatagg gaacccaaaa tagcaagaaa
     1501 cttgaggtat ttttgctaaa aaaaaaaagc attttactat tttgtatata tatatatata
     1561 ttataagata agttttttat aaagacacgc aaatgagtat aaatcagcta atttttggtt
     1621 tacattccta tttttatgca taattattct gtcattctct aatttgtgaa tcaatatatc
     1681 tgattagaaa actaccattg agtttttgct ccacctcatt agtccgctca aaacgccgtc
     1741 cgctaatctt gtcatagact ttctcccctc taggagcccc tgcataagga cgatagaggt
     1801 agcggttgat tcccgtttcg tcaatatata ggactggtaa atcttttagg ttatccaaaa
     1861 tatcaagaaa ctcagaaact ttctcaggct tttgttccct aaaactggtc gtctttttta
     1921 aaatgacctt aatctgcttt aaaactgccc atacggaggg caaagcacaa tcaaaacggg
     1981 ccgcaatttc ccgtaaaaaa gcgtctggat gagcctctac aaaggctttc aattcttcta
     2041 aagggatctt acgctttttg acgactcgct ttttccgctc taagttccct tgctcacgtc
     2101 tttcttttcc cacgtgaaga gagttctgac gccgacatca aaaactttgg ccgcctcgac
     2161 atgtctgtgc cctcctttga tggaatctaa tgctccttgt ttaaaatcag tactatatgc
     2221 catagcttta ttagaccatg cttattttaa actaagcaac tatagataga gaaatgaaat
     2281 aaaatctgaa ttcgaatgtg ataaggagtt agaaattgaa acaataggat tcccccatta
     2341 ttcacacaaa aataaaacca ctaaaaaaca gagcttgcca gcattcttga aaagtagtat
     2401 aattattgca tgcgcaatca tcttgtgacg cagagactgc ccgttaatgg actttcttct
     2461 attttatgac tctaaaaaga aaggagacac tatgacctat ttggaaaaat ggtttgactt
     2521 caatcggcgt cagaaagaaa ttgaaagtct cttggaagag accattgccc aacagagcga
     2581 gcaaagtctg accttgaaag agttctacct gctctactat ctggacttgg ctgaagaaaa
     2641 atctctacgc cagattgacc tgccagataa gcttcatctg agcccgagcg cagtttctag
     2701 gatggtggct cgcttggaag ctaaaaattg cggtctactc agtcgcatgt gttgccatca
     2761 agatagacgc tctagtttta tctgcctgac aaatgatggg caaaagacac tggcctcgct
     2821 acaaaagact gtcgaagaaa gcttggaagc aggtttggat ttcttgattt aagatgattt
     2881 ttaaaaaagt tgcgtgcgca atcatttttc ttgacattct cttttataag gagtaaaata
     2941 aagttagcat taaacaaagg agttttaaat atgattgaaa ttacctatat agatgccagc
     3001 aagaacgaaa gaactgtgac tttcgagtct tatgaagact ttgaacgttc gcaacaagct
     3061 tgccttatcg gcgtcgcaga ctactaccct gtccaaaaat taacctacaa gggtcataat
     3121 ttggactacc atgggactta tggagatatc ttcttctatc tcatgaaaca agatttaagc
     3181 caatataact aaaaaaagga gaaatacaat ggcaaaagca attacagatg caacattcga
     3241 acaagaaaca aaagacggtt tggtcttagt agacttctgg gcaacttggt gtggtccatg
     3301 tcgtatgcaa ggtccaatct tggacaaatt gtctgaagaa ctttcagaag atgtcttgaa
     3361 aatcgttaaa atggacgttg atgaaaatcc aaacacagct cgtgcttttg gaatcatgtc
     3421 tattccaact cttctcttca aaaaagacgg ccaagttgtc aaacaagttg caggtgttca
     3481 cacagcagaa caaatcaagg ccatcattgc tgaattgagc taatcaaacg agagaccaag
     3541 tactttcttt ggtctctttt ttcttgccct ttgccatttt tcaaaaaata tgctagacta
     3601 tagtagattg aaactagaat agtaca
//
LOCUS       DAVMMR010000103         3602 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMMR010000103 DAVMMR010000000
VERSION     DAVMMR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.103"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..278
                     /locus_tag="VYS74_001980"
     CDS             <1..278
                     /locus_tag="VYS74_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789960.1"
                     /translation="RGFTHYKYYRAENSHLIYADYFEMKLQKLLKDDTKVFEKSTFKF
                     VEGYKIYLIESKESGIKQMDNVIKYFEFIESKSIALYFQKRLNELID"
     gene            complement(633..1166)
                     /locus_tag="VYS74_001981"
     CDS             complement(633..1166)
                     /locus_tag="VYS74_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV3789961.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1255..2031)
                     /gene="recX"
                     /locus_tag="VYS74_001982"
     CDS             complement(1255..2031)
                     /gene="recX"
                     /locus_tag="VYS74_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV3789962.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2069..3445
                     /gene="rlmD"
                     /locus_tag="VYS74_001983"
     CDS             2069..3445
                     /gene="rlmD"
                     /locus_tag="VYS74_001983"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3789963.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     LEKVVDKV"
     gene            3485..3556
                     /locus_tag="VYS74_001984"
     tRNA            3485..3556
                     /locus_tag="VYS74_001984"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3518..3520,aa:Glu,seq:ttc)
BASE COUNT         1188 a          608 c          640 g         1166 t
ORIGIN      
        1 ataggggatt tacccactac aaatattata gagccgaaaa ttcacatcta atatatgcag
       61 actactttga aatgaaatta caaaaattat taaaagatga cacaaaagtt tttgaaaaat
      121 ctacattcaa atttgtagaa ggatataaaa tatacctgat agaatctaaa gaatctggaa
      181 ttaaacaaat ggacaatgtc ataaaatatt ttgagtttat tgaatctaaa agtattgctt
      241 tatattttca aaaacgatta aatgagctga tagattaaat atcattttct ctgttaagat
      301 attgttttta aaatattgta ctaaatgatt gatgctatgt ggaaatacaa aaaaatgttt
      361 ttgatacgaa gttgacctgt attttttata ctaatcattt tcgtattttt tgtattaaac
      421 gatataagtt tgttgtaaac ttacaaggaa taaagacatt aaaaaataac agtatatcta
      481 tttgttttat atattttacg aattttgcat aaatctcttt ctagtaatgt gttgtaactc
      541 tgctataata gatttattcc tttttgtgtt tacacaattt attttatact accaaaaaaa
      601 ggtcaggatt ttgttcctga cctttgacaa ctttaccgat tctttagttc tacatagcgc
      661 ttgtaccaaa tgtttacata ggcttctgag aaaggaccac gtccattgtt aatccaatca
      721 acaagaattt tgacatgttc ttttaaaata tagtccaagt catcagaata attcattttg
      781 cgtttgtgac gctcgtactc ttcaacgtcc aagagacgtt tttccccatc tgtaaaaatt
      841 ttaacatcca aatcgtaatc aatatacttc agtgcttctt catccagata gtaggggcta
      901 gccatattgc aatagtaaga agttccatta tcacgaatca tggcaatgat attaaaccaa
      961 tatttcttgt gaaagtaaac aatagccggt tctcgagtga cccaacgacg accatcactt
     1021 tcggtaacaa gtgtatgatc gttgacacca ataatggcgt tttctgttgt ttttagtacc
     1081 atggtgtccc gccaagttcg gtggagactc ccatcatgct tataactttg aattgtaata
     1141 aagtcgcctt cttttggaag cttcataact aaccaacttt ctacaattta taagtttatc
     1201 atttactatt gtaccataaa attacctaaa atctgtgaat ttcacttgga aatattaaag
     1261 atattctcta agagcgcttg ctatatccga aaaatcgtag ccctttcgtg ctaaaacttg
     1321 agttaaacgc tgcttcagtt cgtatccttc atactttcga gcatacttag tatattgctt
     1381 atcaagttcc ttgaagatga gttcttgagt cgtttcttgg tcaacttggc tatctaagtc
     1441 gtcaaaggca attttagcat cagaataaga gaagcccttg ttagtcaagt tctggataat
     1501 cttatcttgc aaggcacgag ctggaagttt tccctcatat tttttcaata gtttattagc
     1561 tacacgttga gcaacttcag aaaaatcaaa ttctttcaag ttctcttcta tagtagattt
     1621 tgaaatccct ttttgagcta gtttctgagt cagtacataa ggacccttgt ctcctgaaag
     1681 ttgattggta ttgatgatag cataagcgta ctgaccatca ttaatccact tatcttcttt
     1741 aagattagca ataacttgag aaacgatgtt tttatcaata tcgtattttt tcagatattc
     1801 tctgacttct ttttcagtgc gtgctttaaa ggataagtgg tagagggcca gattcttacc
     1861 ataagaaaat tgagcaaagt cttgaatctc tttcaattcc tcttcgctta tcaccttatc
     1921 tctcgataac ataaaacgaa caattgtatc ttcggtgata tagcatttgt cgccattatc
     1981 aagctccatc agatagagtc tttttttctt ttcaagtttt gtgattttca tagctctatt
     2041 ataactcaaa atgtgataag ataggggtat gaatctgaaa gtgaaacaaa aaataccatt
     2101 aaaaatcaag cgcatgggaa ttaacggtga gggaatcggc ttttaccaaa aaacattagt
     2161 ctttgtacca ggagctctca aaggcgaaga tatctattgt cagattactt ctattagacg
     2221 caactttgtt gaagcaaaat tactgaaggt caacaagaag tctaaatttc gaattgtgcc
     2281 atcttgtact atttataatg aatgcggagg ctgccaaatc atgcacctgc attatgataa
     2341 gcagctggag ttcaagacgg acttacttca tcaagcgctg aaaaaatttg ctcctgcagg
     2401 atatgaaaat tatgaaattc gtccaactat tggaatgcag gaaccaaaat attacagagc
     2461 taagttacaa tttcagactc gaaaatttaa aaatcaggtc aaggcgggct tatatgcaca
     2521 aaactctcac tatttagtag agttgaaaga ctgcctggta caagataagg aaacccaagt
     2581 gattgctaat cgcttagcag aattacttac ttatcaccag attccaatca cggatgagag
     2641 aaaagttcta ggtgtccgta ctattatggt ccgacgcgcg agaaagaccg gacaggttca
     2701 gattattatt gttacaaacc gccagcttaa tttaactcaa ttggtaaaag agttggttaa
     2761 agatttccca gaagttgtga cagtagctgt taatacaaat acagctaaaa ccagtgagat
     2821 atatggtgaa aagacagaga ttatctgggg gcaagagagt attcaagaag gtgtactcaa
     2881 ttatgaattt tcactatccc ctcgagcttt ttatcaacta aatcctgagc aaacagaagt
     2941 cctctatagc gaagcagtaa aagcgctgga tgttgataaa gaagaccatt tgattgacgc
     3001 ttattgtgga gttggaacga ttggatttgc ctttgcaaag aaagtaaaaa cactcagagg
     3061 tatggatatt attccagaag ctattgaaga tgccaagcga aatgctaaaa gaatgggatt
     3121 tgacaatact cattatgaag ctggaacggc agaagagatt attcctcgtt ggtacaagga
     3181 aggctaccga gcagatgctt tgattgttga cccaccacgt acaggtctgg atgataagtt
     3241 attagatact attcttactt atgtaccaga aaaaatggtt tatatttctt gtaatgtttc
     3301 gaccttggct cgtgatttgg tacgcttagt agaagtctat gatcttcatt atatccagtc
     3361 ggtcgatatg ttcccacata cagctcgaac tgaagctgtt gtaaaattaa taaaaaaatt
     3421 agaaaaagta gttgacaaag tttgaaaaga ctgtataata gtaagagttg aaaataacaa
     3481 ctcaggtccg ttggtcaagg ggttaagaca ccgccttttc acggcggtaa cacgggttcg
     3541 aatcccgtac ggactatggt atgttgcgtc aggaccactt gatgaaaaaa aagtttaaaa
     3601 aa
//
LOCUS       DAVMMR010000104         3422 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMMR010000104 DAVMMR010000000
VERSION     DAVMMR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.104"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            262..1613
                     /locus_tag="VYS74_001985"
     CDS             join(262..703,703..1613)
                     /locus_tag="VYS74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV3789964.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            complement(1848..2456)
                     /locus_tag="VYS74_001986"
     CDS             complement(1848..2456)
                     /locus_tag="VYS74_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV3789965.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2622..2792
                     /locus_tag="VYS74_001987"
                     /pseudo
     CDS             2622..2792
                     /locus_tag="VYS74_001987"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2781..3392
                     /locus_tag="VYS74_001988"
     CDS             2781..3392
                     /locus_tag="VYS74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3789966.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1276 a          435 c          648 g         1063 t
ORIGIN      
        1 agttatggat gtcgttccaa tatgtgtacg ttggaatgtt agtgcttata tggggggaac
       61 agaatcctct cttgattgaa gacaagttag ccattaggct ggtttgtctc tttgtcaact
      121 gtagtgggtt gatataatag tattagtgag tgggataaaa gtttcattta gtttattcag
      181 tacaaattta acgggtcaag atttattgaa ctgcacccca atagttagac agaaaaaatc
      241 taactttagg ggtgttttat tatgaaattg agttatgaag ataaagttca gatctatgaa
      301 ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct
      361 ggtctaaagt acatggtgaa attaattgac cgttatggca tagaaatcgt taaaaaaggg
      421 aagaatcgtc actattcttc taaattgaaa caagaaatga tggataaagc cttactggaa
      481 ggttgttcac aaagaagtat tagtcttgat tatgccctcc caaaccaagg aatgctttca
      541 ttttggccgg cacaatacaa gaaaaacggg tatactattg ttgagaaaac aagagggaga
      601 ccagctaaaa tgggacgtaa acggaagaag acttgggaag aaatgacaga actagaacga
      661 ctccaggagg agaatgaacg cttacggact gaggtggcct acctaaaaaa gttaaaagag
      721 ttagaggaaa gggacgaagc cctagagcga gaaaggcaga gacagttaga gaaatggttt
      781 caggaggatt tcgactagat tttcttcttg aaacagcacg tttagctcgc tcgacttact
      841 attatcagtt gaaacaactg gatggggttg ataaagataa agagattaaa accgaaattc
      901 agggcattga taatgaacat aaaggcaact atggctatcg tcgaatccat ttagaactaa
      961 ggaatcgtgg ttttgtggtc aatcataaga aagttcaacg gttgatgaga attcttggtt
     1021 taacggctcg aattcgccgt aaacggaagt attcttccta ccaaggagag attggcaaga
     1081 aagcagagaa tctcattcaa cgccagttcg aagcatcgag gccaatggaa aagtgctata
     1141 cagatgtgac agagtttgcc attccaaata gcacacagaa actctattta tcaccagttt
     1201 tagatggttt taacagtgaa atcattgctt atcatctttc tacttcgcca aacttagaac
     1261 aagtgaagag tatgttagaa caggccttta cagagaaata ctatgagaat acgattctcc
     1321 atagtgacca aggctggcaa taccaacacg attcttatca tcggttccta gagagtaagg
     1381 aaattcaagc atccatgtca cgcaagggca acagcccaga caacggcatg atggaatctt
     1441 tctttggcat tttaaaatcc gaaatgtttt atggctatga gaagaacttt agatctttag
     1501 aaaaccttga acaagctatt gtggactaca ttgattacta caacaacaaa cgaattaagg
     1561 taaaactaaa aggacttagt tctgtgcagt acagaactaa atcctttgga taaattattt
     1621 gtctaatttt ttggggtcag tacagtatta aataccatga tcttatgcgt tttataataa
     1681 aaaatctgtt cttgttttat ttttagaaaa tttactaaag actaaaaata ttatgaataa
     1741 tttaaaaaat agtcaggtta tatagttttt tgaaattaaa attattttat cgttgaaatt
     1801 agttaaaaat ttttataaaa tcaagatgca aggctatgag atgttctcta gtttaatttt
     1861 tcatacttcg agaatataaa gttgtataaa aatgcattag catattttgt tattgaggga
     1921 agtataaaaa tcattaaagt tatccaaact agtaattgat tctgtaatat aaagaagagt
     1981 agaactggga taatttcaat aattctaata agaacggcaa taaaaaaatg tttataggct
     2041 aacatgattg aattgtgtag agaatttttt aaagtgttat tatatctacc gattaaagga
     2101 attagaaatg ttgttgctat taaatgaagt atgatatata ttactccaat atgtcgtaag
     2161 atagaaatga tatatatagg taatggtatg tagggtagga agaacaaact gaataataag
     2221 attaaaattg gtaaattaga tattaatgct gctcctaaat ttttccaata atctttaaaa
     2281 aaactgtttg cgaagtctct atagggatag gattcctcgt gtcgtacaat ttttattaga
     2341 gttgctatta gggcactttc acctgatccg atagcaataa ctgttactac tgacgctaca
     2401 tagagtagat ttaacattat aagatttgaa actttatcaa taaattttat ctgcatcttt
     2461 aactcctgct gtttttatac ttataatatc acatcatttg agtttcatca actttgaata
     2521 cattaagaaa gaatataact atgaggaaag gaaaaaatta ttatgaataa atccaaaaaa
     2581 tataaagaaa gaattcatta tgaatacgat aatcttacta aatcagagaa aaaattgctg
     2641 actatatttt agttagagat agtgaaatag tttatgaaac gtcttcagaa attgttagta
     2701 aagtaaaagt tggagaggga actcttaatc gattttgcaa aaagattgga tactctggtt
     2761 ttcaggaatt aaaattaaaa atgacgaaag atatattaga gttagaatct caaaaaatga
     2821 gtagtgacac atttattgat gaaataaaaa ataattattt atctattgtg gaaagtacaa
     2881 gaaaattaat tgatggtaga caaatagaat tagctataaa attaataaga gaagcaaacc
     2941 agattttaat gattggtgtg ggttctagtg gaaatgcggc tagagaattt gagagtagtt
     3001 tgttaagaat tggaataata agtaagacgg taattgatac ccattttcaa ctcatgcata
     3061 ctgcgttatt gaaagataat gatttgataa tagcgtttag tttatctggt tccacaaaag
     3121 aagttgagga aactttatta aacgcaaaaa gaaaaaatgt taaaattatt tcaataacaa
     3181 attattcgtc cagaaatata gcaaaattat ctgactgcgt tcttttgaca tcaaaaaaag
     3241 aatcttattt agagggtggt agtttaatgg ctaaggcttc tcaactattc ataattgatg
     3301 taatatgcac tagactctct ttaataaatt atgaagatac tatatgtaaa aaagaagaga
     3361 ttgcttcttt gctgtcaaat aaagtggaat aaatcaataa tttttaaatt ttaataaaaa
     3421 tt
//
LOCUS       DAVMMR010000105         3420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMMR010000105 DAVMMR010000000
VERSION     DAVMMR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.105"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            153..2786
                     /gene="polA"
                     /locus_tag="VYS74_001989"
     CDS             153..2786
                     /gene="polA"
                     /locus_tag="VYS74_001989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV3789967.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2871..3308
                     /locus_tag="VYS74_001990"
     CDS             2871..3308
                     /locus_tag="VYS74_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV3789968.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          932 a          600 c          868 g         1020 t
ORIGIN      
        1 ttatagattc aatatattat attatagcac atttttaaat ctaggtttac taaattctct
       61 ggctctatct atttattcgg aaaaagtttg aaaaatactt gctctagctc ttcccaatgg
      121 tattttttga ttctttcctt tataatgggt gtatggataa gaaaaaatta ttattgattg
      181 atgggtcttc tgtagctttt cgggcgtttt ttgcgctgta tcagcagttg gaccgtttta
      241 agaatgcggc tggtttgcat accaatgcga tttatggttt tcagttgatg ttgagtcatt
      301 tgttggagcg ggttgagccg agtcatattt tggtggcttt tgatgcggga aagacgacct
      361 tccggacaga gatgtatgcg gactataagg gtggtcgggc caagactcct gatgagtttc
      421 gtgagcaatt tcctttcatt cgtgagttgc tggatcatat ggggattcgt cactatgagc
      481 tggctcagta tgaggcggat gacatcattg ggacgctgga taagctagca gagcaggatg
      541 gttttgatat taccattgtc agtggggaca aggatttgat tcagctgacg gatgagcata
      601 cggtggttga aatttccaag aaaggtgtgg ctgagtttga ggcctttacg ccagattacc
      661 tcatggaaga aatgggcctc acaccagctc agtttatcga tctcaaggcg ctcatgggtg
      721 ataagtcgga taatatccct ggggtgacca aagtcggtga aaagacgggt attaagctct
      781 tgctggagta tggttcgctt gaggggattt atgaaaatat tgatggaatg aagacttcta
      841 agatgaagga aaatctcatc aatgacaagg aacaggcctt tttgtctaaa acactagcga
      901 ccattgatac caaggcaccg attgcgattg gtttagagga cttggtctat agtggtccag
      961 atgttgaaaa tcttgggaaa ttttacgatg agatgggatt caaacagctc aagcaggctt
     1021 taaatgtgtc gtcagctgat gtggctaagg gtttggattt tactattgtt gaccaaatca
     1081 gtcaagatat gctgagtgaa gagtctatct tccactttga gctttttggt gagaattacc
     1141 atacggataa tttggttgga tttgcctggt cttgtgagga taagctctat gccacagaca
     1201 agcttgagct gttgcaagac ccgattttca aggatttctt agaaaaaaca tctctgagag
     1261 tttatgactt taagaaggtt aaagttcttt tgcaacgttt tggtgtggat ttgcaggcgc
     1321 ctgcttttga catccgtttg gctaaatacc tcctttcgac tgtggaggac aatgaaattg
     1381 cgaccatcgc tagtctttat ggtcagactt acttggttga tgatgaaact ttctacggta
     1441 agggtgttaa aaaggccatt cctgaacgtg agaaattctt ggaacactta gcttgtaaac
     1501 ttgctgtttt ggtagaaaca gagcctattt tacttgaaaa actcagcgaa aatgggcaat
     1561 tagagcttct ttatgatatg gagcaacctc tggcttttgt tcttgccaag atggaaattg
     1621 ctgggattgt ggtcaagaaa gagaccttgc ttgagatgca ggctgaaaat gagcttgtca
     1681 ttgaaaaact gactcaagag atttacgagc tggctggtga ggagtttaat gtcaactcgc
     1741 ctaagcagtt gggcgtgctt ctctttgaga aattgggact tcctctagaa tacactaaga
     1801 aaaccaagac aggttattcg acagcagtgg atgtgttgga gcgtctcgct cctattgctc
     1861 cgattgttaa gaaaatcctg gattaccgtc aaattgctaa gattcaatct acttatgtaa
     1921 ttggcttgca ggactggatt ttggctgatg gaaagattca tactcgctat atgcaggatt
     1981 tgacccagac cgggcgtttg tctagtgtgg atccaaactt gcaaaatatt cctgcccgat
     2041 tggaacaagg gcgcttgatt cggaaggctt ttgtgccaga gtgggaggat agtgtgctac
     2101 tcagctctga ctattcacag attgaattgc gcgttttggc gcatatttct aaggatgagc
     2161 acttgattaa ggccttccaa gagggggcag atatccatac ttcgacagcc atgcgggtct
     2221 ttggcattga gcgtcctgat aatgtgactg caaatgaccg tcgcaatgcc aaggcagtta
     2281 actttggagt ggtttatggg atttcagact ttggcttgtc taataatttg ggaattagtc
     2341 gtaaggaagc caaagcctac attgatacct actttgaacg ttttccagat attaaaaact
     2401 acatggatga agtggtgcgg gaggcgcgtg ataagggcta tgtagagacc ctctttaagc
     2461 gtcgccgtga gttgccagat atcaattcgc gcaacttcaa tattcgtggt tttgcggagc
     2521 gaactgctat caactcacct atccagggtt cggcagcaga tattctcaag attgccatga
     2581 ttcagctgga taaagccttg gttgcaggtg gttatcagac taagatgctg ttacaagtgc
     2641 acgatgaaat cgtccttgaa gtgcctaaat ctgaattggt agagatgaaa aaattggtga
     2701 aacaaaccat ggaagaagcc attcaactca gtgttcctct tatcgcagat gagaatgaag
     2761 gggcaacctg gtacgaggct aaataaaaag ggggctagtc ctcctttttt gtagtataat
     2821 tctgcaaacc ttttcaaaat gtgctatact gatgaaaaag gaggatttct atgagtcaag
     2881 aatttatcaa tccaagtgat ggcgtgattc gtcagtatct cgcaacgagt aaaacccttg
     2941 ctgtggtggg tttgtctgac cgtgaagaaa caaccagcaa tcgagtaaca aaggaaatgc
     3001 aggctcgggg ctataaaatc atcccagtca atcccaaggc ggcaggtggc gaaatcttgg
     3061 gtgaaaaggc ttatgctagc ctcgctgaaa ttccttttcc tgtagatatt gtcaatgttt
     3121 atcgtcgcag tgaatttctg cccgatgtgg cgcgtgattt tctcaaagct gatgccaaga
     3181 ttttttgggc acagctagga cttgaaagtt tagaagcgaa agagattttg cgtgatggtg
     3241 gatgtgatga tatcgtgatg aatcgctgta tcaagagaga acatactcgc ttgattgagg
     3301 aagcataaga aaaaggtagc tgatgggcta ccttttgtgt tatactcaat gaaaatcaaa
     3361 gagcacacta gcaaactagg aaactagccg cgggctgctc aaaacaccgt tttgaggttg
//
LOCUS       DAVMMR010000106         3200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMMR010000106 DAVMMR010000000
VERSION     DAVMMR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.106"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..1059)
                     /locus_tag="VYS74_001991"
     CDS             complement(<1..1059)
                     /locus_tag="VYS74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV3789969.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEESPVASQSQAEK
                     DYDAAMKNAEDAKKALEDAKAAQKKYADDQKKTEEKAKKEKEASKEEQAANLKYQQEL
                     VKYASEKDSVKKAKILKEVEEAEKEHKKKRAEFEKVRSEVIPSAEELKETRRKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIGES
                     DSEDYVKEGLRAPLQSELDAKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     YSALYLEAAEKDLAAKKAELEKTEADLKKAVNEPEKPAEEPENPAPAPKPAPAPQPEK
                     PEKPAEQPKPEKPEKPAEQ"
     gene            complement(1607..1846)
                     /locus_tag="VYS74_001992"
     CDS             complement(1607..1846)
                     /locus_tag="VYS74_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789970.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTIPAIQAGVFILEDFRNILCTMM"
     gene            complement(2982..>3200)
                     /locus_tag="VYS74_001993"
     CDS             complement(2982..>3200)
                     /locus_tag="VYS74_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789971.1"
                     /translation="IKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGY
                     LFSVLLLFSNFSVPFIKEKEYELFKNSK"
BASE COUNT          954 a          636 c          447 g         1163 t
ORIGIN      
        1 ttgttcagct ggcttctctg gtttttctgg ttttggttgt tcagctggct tctctggttt
       61 ttctggttga ggagccggcg ctggttttgg tgcaggagct ggattctcag gctcttcagc
      121 tggtttttct ggctcattaa ctgctttctt gaggtcagct tcagtttttt ctaattcagc
      181 ttttttagca gctaaatctt tttctgcagc ttcaagatat aatgcagaat attcaccgtc
      241 tgagtttttg aaatcttcta catctttttc aagttttgca atttcagcgt ctaactcatc
      301 aatcttatca ctcaactctt caagttttga tagtttagct tgcttagcat ccaattcaga
      361 ttgaagagga gcacggagac cttctttaac ataatcttct gagtcagact caccaatctc
      421 tttgagagct ttttctagtt tctgaacttc atgttccaac tcagcgattt tagcttgagg
      481 agcgacttct tcagcatgtt ctgcatccac tttttgtttg gcttcagtaa cttttttctc
      541 agcttcttct acttttttag taagttctgc ttcttttgct ttagcctctt ctgcttttcg
      601 tctagtctct tttaactctt ccgcgctagg aattacctct gatctaactt tctcaaattc
      661 tgctcgtttt ttcttatgct ctttctcagc ttcctccact tctttcagaa ttttagcttt
      721 ttttactgaa tctttttcac tagcatattt aaccaactct tgttgatatt tcagatttgc
      781 agcttgttcc tctttagaag cttctttctc ttttttcgct ttctcctcag ttttcttttg
      841 atcatcggca tattttttct gcgcagcttt tgcgtcctct aaagcttttt tcgcatcctc
      901 agcatttttc attgctgcat cgtagtcttt ctcagcttga gactgactag ctacgggaga
      961 ttcttctgct cttacaaaag taggctgaga cgcaacaaaa ccagccccta agatagcgac
     1021 gctggctaga cttgttaaaa tcattttttt cttattcatc taaatttacc tcttttctga
     1081 tagtcatttt tagactatac ttatattaag tatactcccc ccgtaaatat ttgtcaaatg
     1141 ttttatatta catttttatt acaaatttct atatcataag cttctactcc aaaaagataa
     1201 agacactact tttgcccttt tcgattctct ttttaaacat agcatcatct tccttcattc
     1261 ctataaaaac atttccgatg agagagcttg ttttttaatt tcgttaaaca acaaaaaacg
     1321 caagcttaag gctgcggaca atctttatag ccatttcatt tctttcacat ctgttttacg
     1381 aactttagca tatttcttag cgcttgtaaa atgtagagta actggtttaa acactccgct
     1441 tttgtctctt cgtaaaccta aaactgcgga tcttttattt ttatcctcaa aaaatatgac
     1501 gtctaatttc atagtatttt agataaatcc ttagctacga tgcaacaact tgttagctta
     1561 gaatagaaga tatcgccgat aaactcatat aaagatatcc tttcgattac atcattgtac
     1621 ataggatatt tctaaaatct tctaaaataa aaacaccagc ttgaatagca ggtatcgtat
     1681 gactagcaaa atctcgtgtg attttgtgta aaccaaataa atgatgtaaa tcagtcaagc
     1741 aaaaattcac tttgaaagtc tctaaatact tataattcgt tgatacttga catgtttttc
     1801 cattgaatct ttcttgataa tccaaaatac aatcaagtaa tttcaacgac aagctccata
     1861 ttttctcaaa ataaaaaagc acggggcagt tctcactact gcccaaagtg gttgattaga
     1921 gtcggcttct ctaactccct atgttgtgct tattaggctt atcgcaaggg aacagttttt
     1981 agctctgcca agccacatag tgtgcttact actatgtgtc catgtcacca tgaattgccg
     2041 aacggtttaa cgaaaaagta tagtagattg aaacaagatg tgaacaaatc gattaggaaa
     2101 gtcaaattaa tttctagaaa tattttaaca gtcataacgg actattccag attcaatcta
     2161 ctatattact tttaggttta tcccaactca attatgacat ctttttttca aaagtcaata
     2221 tatctcactc ttttcaacga caagaaagag gctgataatc gaccaacctc tgtttttaga
     2281 tacagttcaa gtccaaacta ttctttatag aaaagtccga taaattcgag caatagttaa
     2341 catacttaca aatataactc ccaaaataga taacactatt ttcacaaaac tcagttccga
     2401 acctatgaca tatatatgaa aaagaggggc tagtcctaca catatataca ataagtagcc
     2461 aacaagtttt atctcaccaa ttcctgcaaa aaaaatgttt catgtaacga ttctccattt
     2521 actccattaa atcagtttta ttttagacta tcataaaaca atcacctctt caataaaaac
     2581 cattggcgaa atactattct gaagcattga tagctcagat ttctcagaat agagctgtcc
     2641 taactatcga gcagctcaaa tacatatata tgagcaattc taagcctaga attgctataa
     2701 aataaatgtt ttcaggctat tctaagtctt tgactgctta aaattttacg ttctgaagga
     2761 ttctaaagct agaatagctc aaaatcacga gaactaaggt attcgaaact tcagagtaat
     2821 acaatcccca taggaattct ccattcttat taagcatgat taaaatgtaa tcctaacaag
     2881 tgatcctcga ttgtgtttgt agcacttagt tacctagata agactatttc cctttatcat
     2941 atctgatttt aagccgttct aagatttaat ccatatttct attacttgct atttttaaat
     3001 aattcgtatt ccttttcttt gataaatgga actgaaaaat tagaaaaaag aagtaaaacc
     3061 gaaaacaaat agcctgcttt ttgaatcact aaaagatatc ctaaaaataa gtaagctata
     3121 acatccaatg cgaaaagaaa aaatattctc ctcttcatta aagtacttgt gtcaaataat
     3181 ctgatgaata aaaattttat
//
LOCUS       DAVMMR010000107         3115 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMMR010000107 DAVMMR010000000
VERSION     DAVMMR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.107"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            124..2253
                     /locus_tag="VYS74_001994"
     CDS             124..2253
                     /locus_tag="VYS74_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV3789972.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            2240..2689
                     /locus_tag="VYS74_001995"
     CDS             2240..2689
                     /locus_tag="VYS74_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV3789973.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            2748..3020
                     /locus_tag="VYS74_001996"
     CDS             2748..3020
                     /locus_tag="VYS74_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3789974.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT          905 a          597 c          714 g          899 t
ORIGIN      
        1 ttatccacaa cctcaaaaca gtattttgag cagcctacgg ctagcttcct agtttgcttt
       61 ttgattttta ttgagtatga aaaggattgg agaaagcctt ccagcttgtg gtataataga
      121 tttatggata aaaaatatga aaaaatctct caggatttgg gagtgacgtt aaagcaaatt
      181 gataccgttc taagtttgac agctgaaggg gcgactattc cctttatcgc gcgttatcgc
      241 aaggacatga ctggtagtct ggatgaggtg gcgattaagg ctattattga tttggataaa
      301 agtctgacaa atctcaatga ccgtaaggaa gctgtcttag ctaagattca agaacaaggt
      361 aagttgacca aggaattgga agaagctatc ttagttgccg aaaaattagc agacgttgaa
      421 gaactctatc ttccttataa ggaaaagcgt cgtaccaagg caaccattgc ccgtgaagct
      481 ggactctttc ctcttgctcg tttgattttg cagaatatag ttgacttaga gaaagaagct
      541 gaaaagttcg tctgtgaagg atttgcgact ggcaaggaag ccttgaccgg tgcagttgat
      601 attttggtcg aagccttatc ggaagatgtg accttgcgtt ctatgactta tcaggaagtg
      661 ctgagacact ctaaactcac ttctcaagcc aaggatgaaa gtcttgatga aaagcaggtt
      721 tttcagattt attatgattt ttcagagaca gttggaacta tgcaaggcta tcgtaccttg
      781 gctctcaatc gtggggagaa acttggtgtc ttgaagatcg gttttgaaca tgcgacggac
      841 cgtattcttg ccttctttgc tactcgtttc aaggtgaaaa atgcttatat tgatgaagtt
      901 gttcagcaat ccgttaagaa aaaggtcttg cctgctattg agcgtcgtat tcggacagaa
      961 ttaactgaga aagctgaaga gggagctatc caactttttt ctgacaatct gcgcaatctc
     1021 ctcttggttg ctccactgaa agggcgcgtg gttcttgggt ttgacccagc ctttcgtaca
     1081 ggtgccaagc tagctgtcgt ggatgcaaca ggaaaaatgc tgacaactca ggttatttat
     1141 cctgttaaac cagcatcagc tcgtcaaatc gaagaagcca agaaagattt agcagattta
     1201 attggtcaat acggtgtaga gattattgcc attggaaatg gaacggccag tcgtgaaagt
     1261 gaagcttttg tagcggaagt tctgaaagat ttccctgaag tcagctatgt tatcgttaat
     1321 gaaagtggtg cttctgtcta ttctgccagc gaacttgctc gtcaggagtt tccagacttg
     1381 accgttgaaa aacgctctgc catttctatc gcccgtcgtt tgcaagatcc tcttgcggaa
     1441 ttggtcaaaa tcgatcctaa gtcaattggt gtcggtcaat accaacacga tgtcagtcag
     1501 aagaaactat ctgagagtct ggactttgtt gtcgatacag tggttaacca agttggtgtc
     1561 aatgtcaata cagctagccc agctcttctt tcacacgtag ctggactcaa taaaaccatt
     1621 tctgaaaata tcgtcaaata ccgtgaggaa gagggaaaaa tcacttcacg ctcccaaatc
     1681 aagaaagttc ctcgtttggg ggccaaggct tttgaacagg ctgctggttt cctccgtatc
     1741 cctgaaagta gcaatatcct tgataataca ggagttcacc cagaaaatta cgctgccgtt
     1801 aaggaactct tcaaatgctt ggatattaag gatctgaatg aagaggccca aagcaaactc
     1861 aagtcgcttt cagtcaagga aatggcgcaa gagttagacc ttggtccaga aactcttaaa
     1921 gatatcattg cagatcttct caaaccaggt cgagatttcc gtgattcctt tgatgctcca
     1981 gttctccgtc aagatgtcct agatatcaag gacttagtgg ttggacaaaa gctagaagga
     2041 gtggtgcgta acgttgttga tttcggtgct tttgttgata tcggaattca tgaggacggt
     2101 ctcatccata tttcccatat gagtcgcaag tttatcaaac accctagtca agtggtggca
     2161 gttggagatt tggtgagcgt ttgggttaag aaaattgata ctgaacgtga aaaagttaat
     2221 ctgtctctcc tagctccaaa tgaaactgac tgattacgtc aagcaggttt cactagaaga
     2281 cttcggcaga ccttttatcc atcatgtcca gtggaatagg cgtctacgtt cgacaggtgg
     2341 gcgatttttc cccaaagatg ggcatttgga ttttaatccc aaggtttatc aggaactggg
     2401 gttggacgtt tttaggaaaa ttgtccgaca tgaactctgt cattatcacc tctattttca
     2461 agggaaaggc tatcaacaca aggatcggga ttttaaggaa ctgttgaaag cagtggatgg
     2521 attacggttt gtaccatcct tgcccaatag taactccaag ccaatcaagc tctatcgttg
     2581 ccaatcctgc cagcaaactt atcagcgtaa gcgtaggatt gataccaaac gctatcgctg
     2641 tggactttgt cgaggaaagt tgctattagt aaatcagcct gaggactgat gaaagccgag
     2701 cctatccgtg atatactatg tctaacctag cagaaagagg aaatatcatg aacagtaagt
     2761 tttataaaat gagacgaaat cgcatgattt caggagtttt agcaggtctg tcagacaagt
     2821 ggaactttga tgttaccctt gtacgttttc tgtttgccat ttttacagtc gcaaactttg
     2881 gacttggggt gattatctat atcatcctag cttctatcat gccaaccaag gaagaaatcg
     2941 aagcagaaat gtacggaaca ggcccacgta aacgcaaaga agctcaagcc attgacgata
     3001 atgaaggctg gttttggtga ggttttacaa aagatttgga atacgcaaaa agctttaaat
     3061 caatagtcta aactgcttga tttaaagctt ttttactttt atactcaata aaaaa
//
LOCUS       DAVMMR010000108         3040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMMR010000108 DAVMMR010000000
VERSION     DAVMMR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.108"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(153..1094)
                     /locus_tag="VYS74_001997"
     CDS             complement(153..1094)
                     /locus_tag="VYS74_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3789975.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1072..2835)
                     /gene="aspS"
                     /locus_tag="VYS74_001998"
     CDS             complement(1072..2835)
                     /gene="aspS"
                     /locus_tag="VYS74_001998"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV3789976.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          885 a          708 c          613 g          834 t
ORIGIN      
        1 tcaaaacatg gttttgaggt tgcagataga actgacgaag tcagctcaaa acatggtttt
       61 gaggttgcag atagaactga cgaagtcagt aacatatata cggcaaggtg aagctgacgt
      121 ggtttgaaga gattttcgaa gagtattaat cactaattat ctatctcaac aaatcttcct
      181 agaatatgaa cgttttccga gacagagaca aaggagcttg gatccacttg tgtcataatc
      241 tgtttaaatt cattaaactc tgcacgtgta atgacagtga ttaaaactgc ctttctctcg
      301 tgattatagg ttccttctgc atcgtggatc atggttgctc cgcggtgcaa ttttttatgg
      361 attttttcaa ttaccttctc tggatgattt gtcacaatca tggcctgcat acgcttttgc
      421 ttagtaaaga ctgcgtctgt cacacggcta gagacaaaga tggtaatcat agaataaaga
      481 gcgtatttcc aaccaaaggt caaacctgct atcagcatga tagttccatt taccaagaaa
      541 gaaatactac cgacattctt acccgttttc ttgcgaatag tcaggctgac gatatccgtc
      601 ccaccactgg agatattgtt tcgaagagca aaaccaatcc ccaaacccat aacaacaccc
      661 ccaaaaaggg aattgataat gggatcctcc gtcaaggttg ccacagggac aaactggata
      721 aagaaggaac tcatagatac cgtgataaag gtaaagacgg tgaacttatg gccaatctga
      781 taccaagcta agaccatcaa agggaagtta atggcgtaga agcttagcga aatcggaata
      841 tgaaaaccaa accagtgatt actcaaggca gagataatct gtgccagacc tgttgcacca
      901 ctcgaataca catgccctgg ttggaaaaag aaattaactg ctactgctga taaaaaacca
      961 tagaccagag aggccgaaat cttctcatca tacttttctc gagagatact ttgtaagaca
     1021 cgtaaaattt ttatctgata agcaaagcgg cgcagataat agcgccaccg cttaattcgt
     1081 tttgtttgtt tcatcttctt ctacttgtaa gctgagttcc tctagttgtt tgagagcgac
     1141 tgttgatgga gcttgtgtca ttgggtcagt tgccttgttg ttcttaggaa aggcaatgac
     1201 ttcacggata ttttcttctc cagcaagcaa catgacaaaa cggtcaagcc cgatagccaa
     1261 accaccgtgt ggtgggaaac catagtccat ggcttcaaga aggaaaccaa actggtcatt
     1321 ggcttcttca gttgagaaac caagagcctt gaacatgcgt tcttgaaggt ctttttggtt
     1381 gatacgaagg ctaccaccac caagctcata accgttcaag acgatatcgt aagcaatggc
     1441 acgaacctta gccaaatcac cttctaattc gtgagcagtc tcttcctgtg gaagtgtgaa
     1501 tggatggtgg gcgctcatgt agcggccttc ttcttcagac cattcaaaca tcggccagtc
     1561 aaccacccaa aggaagttga acttatcatt atcaatcaag ccaagctctt tagcaatacg
     1621 tccacgaagg gcacccagtg ttgcattagc cacttcaagc gtatccgcca caaagagaac
     1681 caagtcctta tcttcaagag caagcgctgt tgtcaattct tcttggatac cagtcaagaa
     1741 cttggcaact ggtccgttta attctccatc aaccaccttg acccaagcaa gacctttggc
     1801 accatactgt ttggctactt ccgtcatctt gtcgatgtct ttacgtgaat agttgtccgc
     1861 agctcctttg accacaatcg cttttacagc aggtgcttct gaaaagactt taaagtctac
     1921 acctttgacc acttctgtca agtcctgaag caacatgtca aaacgagtat ctggcttgtc
     1981 agaaccgtaa agagccatag catcatcgta tttcatacga gggaacggta gcgttacttc
     2041 gatgcctttt gtttccttca tcacgcgcgc gatcaagcct tctgtaatat cttggatttc
     2101 tttctcagta aggaaggacg tttccaagtc gacctgagta aattcaggct ggcggtctcc
     2161 acgcaagtcc tcgtcacgga aacatttaac gatttggtag taacggtcaa aaccagcatt
     2221 catcaagagc tgtttcgtga tttgtggact ttgaggaaga gcgtaaaaat gccccttatt
     2281 aacacgagac ggcactaaat aatcacgcgc cccttcaggc gttgacttag aaaggaatgg
     2341 tgtctccacg tcgataaact ccaactcatc caaatagttg cggatagagt gggtcacctt
     2401 ggcacgaagt ttaagatttt ccaacatttc tggacgacga aggtcaaggt aacggtaacg
     2461 caaacgtgta tcgtcatttg cctcaatgcc atccttaatc tcaaatggtg ttgtcttagc
     2521 tgtgttaagc acaataagag ctgtcacgtt taactcaacc gcaccagttg gcaacttatc
     2581 attggcttgc tcacgcgcag cgacctgacc agtcacctca ataacaaatt cgctacgaag
     2641 gctttcagct gttgccataa cctctgcaga tactttttca gggttgataa ccaactgcat
     2701 gattccttca cggtcacgaa gatcgataaa gatcaaacca ccaaggtcac gacgacggcc
     2761 aacccatcct ttcaaggtta tttcttgtcc gatgtgttcc tcacgaacac gaccagcata
     2821 catactacgt ttcattattt ctctcctctt ttattctgtt actattttac cataaaagcg
     2881 caggctcttc atgaaaatca tcagaaaagt ttgccagtct ttaaaagtca agtgaaagcc
     2941 ctaaaaatta gcgctaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac
     3001 cgtaggtatg gttactgact tcgtcagttt catctacaac
//
LOCUS       DAVMMR010000109         2723 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMMR010000109 DAVMMR010000000
VERSION     DAVMMR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.109"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            266..580
                     /locus_tag="VYS74_001999"
     CDS             266..580
                     /locus_tag="VYS74_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789977.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            590..796
                     /locus_tag="VYS74_002000"
     CDS             590..796
                     /locus_tag="VYS74_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3789978.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            796..993
                     /locus_tag="VYS74_002001"
     CDS             796..993
                     /locus_tag="VYS74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789979.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQGDQFVWLYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            996..1730
                     /locus_tag="VYS74_002002"
     CDS             996..1730
                     /locus_tag="VYS74_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV3789980.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1742..2383
                     /locus_tag="VYS74_002003"
     CDS             1742..2383
                     /locus_tag="VYS74_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3789981.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2401..2631
                     /locus_tag="VYS74_002004"
     CDS             2401..2631
                     /locus_tag="VYS74_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789982.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          807 a          452 c          559 g          905 t
ORIGIN      
        1 aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agtaaccata
       61 cctacggcaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat taatgataga
      121 taaagtggtc aggaacttac tcctgacctt tttcttttgc aaaatgacaa aaatcataaa
      181 ctttttgaca aatatacttg tcaaaaagaa aaagatatgt tacaatgaat tcaagttaag
      241 aaataggaag cagaaaggag ttataatgtg ggtactagga tttatactat ttatgatttt
      301 cttttattct aataattcta aaaaaatcaa gaaactagag aataaaatca aaaaacttga
      361 gcgaaaagag aaaggaaacg cagaaatgtc gagattatta caagaaatga ttggaaagga
      421 accaattata acgggagtgt atattgggcc agataactgg gaagttgtgg atgttgatga
      481 ggaatgggtc aaactacgta gtgttgataa tacgggaaaa gaaaaattca agttgcaacg
      541 tattgaagat atccaaaccg ttgaatttga cggagagtag gtgtgtaacg tgcaactaaa
      601 aaatcgtcta aaagagcttc gagctcgcga tggtctcaat caaaccgacc tagccaaact
      661 ggcaggggtt tccagacaga ccattagcct actagaacgg gatgagtaca ccccatccat
      721 tatcattgcc ctgaaaatat ctcaaatttt caacgaaaca gtcgaatcgg tatttcgttt
      781 ggaggaggac gagtgatgaa caagtataaa gtgatttatt atgtagtggt catagcttta
      841 ttagtcagcg tatttctact gattgggatg gacctaagct ggtttaatcc ctatcaaggc
      901 gaccaatttg tttggctcta ctttgctctc atcccagtaa ttgaatggat tgaaaagaaa
      961 tccaaaaatc tagcaagtga aaaaggagaa tgaatatgaa aaagaaacgt agattgttat
     1021 ttttgatgtc tattgtcttg ggggggttct tgggcatgtt tgtagggatg tttaaggcac
     1081 gtgtcgaatc ccacgaaatt attttagatg taaaagcctt gatgccatgg atatcagcta
     1141 tttgtttact gataggtttc attagtatgt ttttgacttt caatttctta aagaaaagca
     1201 gaaaatttca ttccttgtat caagaggaaa tggatgacga tctgaatgaa acctattatg
     1261 tgcaaatgta tcggaatctt gagtttggaa ccattgcttt taatattaca ggtgtagcga
     1321 ttccattggc tatttttatt tcattaagtg aggtgattat attgcataca aaccctcaaa
     1381 catttttcct ttctttctta ctctttgtgg tattcttagt cgctcaaaaa tctcttttta
     1441 aaaccattgc gattgttcgt cagtttgatt tggaattttt cgctacacca aaggatgtct
     1501 tgaactatat aaattcttat gatgaagggg agcgtcaggc taatttggaa cagagttttc
     1561 gaattttatt ccaattacac caatatgtct taccagcctt atatattttt cttattatca
     1621 tttctttctt gacaggagag attcagttac tagctttctt gcttgtagga gccatccatg
     1681 tttatatcaa tgtgatgcag ttacctatgg taaaacgtta tttcaaataa aggagttatc
     1741 tatgaaatta cttaaaaacc ttggctggat tcttctagcc cttctatcct ttttatttat
     1801 ctatggcttt attcaggggc tcgcgactgc gtcgcttgct ttaggcgctt caccctatgc
     1861 tgttactcta ctctatgtgg ccttggctgg agtatatgtg tacggcattt acaaatggta
     1921 tcagaaggct cctgttcata ttgagaagag cggctttaac cgatttattt ggcttcctgc
     1981 cttggtttgg ttcttatctc tggtcgttca attcttcttg ccagatgatc cttcagtaaa
     2041 tcagcaaata gcgacagact tgaccttgtc tcaaccactt ttctcattct ttgctgtggt
     2101 tatttttgct cctttgacgg aagagattgt ttttagaggg atgttagcac gctatctctt
     2161 tcctaagcag gacaatagta aacgaaccct gatttttctt ctggtatcta gtcttctatt
     2221 tgccttgatt cattttccag gtgatgtgca acaatttttt gtctatttta gccttggttt
     2281 tagtttgggc ttggcttaca ttagcagaaa aggtctggtc tacagtattt ctcttcacgc
     2341 tttgaataat ttagtcggct ttttgatgat tctcatgcta taatagagtc aggaggtcac
     2401 atgaaacgag taattttatt agcagtgatt caggcagtcg ttctattttt catcattgga
     2461 gcgctagctt atgccttcaa aggcgatttc ttctataact atctagcagt tgtctttgct
     2521 cctattgcag gtgtactgcg ttttgggacg gcttacataa cggaaattgt cttgcctcga
     2581 aaggcagccg aaatcgctga aaagcgtaaa gcaggcaaaa attcaaaata aaagagtccg
     2641 gtgaagttca tcggattttt gtattatact caatgaaaat caaagagtaa actaggaagc
     2701 tagccgcagg ttgctcaaaa cac
//
LOCUS       DAVMMR010000110         2658 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMMR010000110 DAVMMR010000000
VERSION     DAVMMR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.110"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     regulatory      72..160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            375..1043
                     /locus_tag="VYS74_002005"
     CDS             375..1043
                     /locus_tag="VYS74_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV3789983.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1325..2107
                     /gene="thiM"
                     /locus_tag="VYS74_002006"
     CDS             1325..2107
                     /gene="thiM"
                     /locus_tag="VYS74_002006"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3789984.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2109..>2658
                     /locus_tag="VYS74_002007"
     CDS             2109..>2658
                     /locus_tag="VYS74_002007"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3789985.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTN"
BASE COUNT          886 a          383 c          580 g          809 t
ORIGIN      
        1 aaagatattt ttcggtcaaa tatcttgcta gggctttcat tttttgctat actggttcag
       61 taattaaata atagagacat ttggggtgct ttaggctgag ataataccca ttgaacctga
      121 tacagttaag actggcgaag ggaaatgtga aacgtttttt cgtatggcga aatgaacggt
      181 ctaatcttgt aagtcaactc taattcttga ttgtaattgg agttttttgt ttatataaac
      241 tggataggct tgtttagtta gctctctgca aatgctctcg atgtgcttta aaaggatcaa
      301 gtggaattac gattttgctt gatatttctt ggtgttttat taattactaa caagtaaaga
      361 gaggagaagt aaatatggaa acacaagact atgcatttca gccaggtttg actgttggag
      421 aattattaaa aagcagtcag aaggattggc aggctgcaat caatcatcgt tttgttaagg
      481 aactttttgc ggggacaatt gagaataagg tcttaaagga ctatctgatt caagattatc
      541 acttctttga tgccttctta tccatgctgg gtgcctgcgt agctcacgca gataaacttg
      601 aatccaagct tcgttttgcc aagcaactag gctttcttga agcagatgaa gatggttatt
      661 tccaaaaggc ttttaaagag ttaaaagtag cagagaatga ctatctagaa gtgactttgc
      721 accctgtaac aaaagccttt caggatctaa tgtattgggc agtggcttca tcagactatg
      781 cccatctttt ggttatgctg gtcattgcag aaggtctcta tttagactgg ggttctaaag
      841 atttggctct acctgaagtc tatattcatt cggaatggat caatctccac agaggtcctt
      901 tctttgcaga gtgggttcaa tttctggttg atgaactcaa tcgtgtcggt aaaaatcgag
      961 aagatttgac agaacttcag caacgctgga atcaagcagt cgctttagaa ttagcttttt
     1021 ttgatattgg ttacgatgtc tagagaaggc tcatgatatt tacaaatttg atgaggtaag
     1081 actcttcgaa aatctcttca aaccacgtca gttctatcca caacctcaaa acagtgtttt
     1141 gagctgactt cgtcagtctt atctacaggc tcaaagcagt gttttgagca acctgcggtt
     1201 agcctcctag tttgctcttt gattttcatt gagtataaat cattcaatgt aaatgaaaaa
     1261 aattctcaac taaagataaa tcgaaattag tagatcgtat aaagtgaaaa ggaaggattt
     1321 caatatgaca agtttaaaat tattaaaaga aaaagcacca ttggtcattt gtataaccaa
     1381 tgatgtagta aaaaatttca cagcaaatgg attagtagca ctgggcgcct caccagccat
     1441 gagtgagttt ccagcagatt tagaggattt gttaaagtat gctggtggtt tattaataaa
     1501 cataggaaca ttgacagatg aaaattggaa attataccaa gctgctctga aaattgcaga
     1561 gaaatataat gtcccagtag ttttagatcc tgtagcctgt ggagcaggag aatatagaaa
     1621 aaaagtagca gatgatttaa tcaacaatta caaactagct gcgattaggg ggaatgctgg
     1681 agagattgcc tctttagtag gaatagatgt ggcatctaaa ggagtagata gtgcgggcgt
     1741 agataatatt gacgaaattg ctctagcagc aaatgagaag ttcaatattc caatagtagt
     1801 aacaggtgaa gtggatgcca ttgctgttaa tggagaagtg gtaacgattc ataatggtag
     1861 tgctatgatg ccgaaagtca ttgggacagg atgcttatta ggagctgtag tagcaagttt
     1921 tatcggactg gaaaaaggtc aagaattaaa atcattagaa acagcaatgt tagtttacaa
     1981 tatcgctgga gaaatggcag aaaaacgtcc aaatggacat cttcctggga catttaaagt
     2041 tgaatttata aatgccttat acgagattac agatgaagat gtaaaggaat tcaaaagagt
     2101 gaagtaagat gtttcataaa gaattactaa aactatattt tatttgtgga acgactactt
     2161 gccaaggaaa aaatctatat acagtcgttg aggaagcctt aaaaggtggt ataaccttat
     2221 ttcaatttcg agaaaaaggt gagggtgctt tagaaggctt agaaaaactt gagctagcta
     2281 tccaaattaa agaactttgt aaaaaataca atgttccatt tatcgttaat gacgatatag
     2341 atttagcaat ggaaattgac gccgatggcg tacatgtagg tcaagatgat attggtgttg
     2401 atgaaattag aaaattgatg ccagataaaa taattggtct ttctataaga aacgaagaag
     2461 aatttcaaca atcaaaagtt gaatatgtag attatgtagg tgttgggcct gtatttgata
     2521 cccagtcaaa agatgatgct ggtggtgcta taggttatga aggtcttgaa ttgatgagaa
     2581 aactattgcc acaaatgcct ttagttgcaa ttggtgggat acagacgaaa catattaaag
     2641 acattataaa gaccaata
//
LOCUS       DAVMMR010000111         2535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMMR010000111 DAVMMR010000000
VERSION     DAVMMR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.111"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..559
                     /locus_tag="VYS74_002008"
     CDS             <1..559
                     /locus_tag="VYS74_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3789986.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYVWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VYS74_002009"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VYS74_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV3789987.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHANYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2023
                     /locus_tag="VYS74_002010"
     CDS             1424..2023
                     /locus_tag="VYS74_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3789988.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(1977..>2535)
                     /locus_tag="VYS74_002011"
     CDS             complement(1977..>2535)
                     /locus_tag="VYS74_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3789989.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT          867 a          364 c          437 g          867 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgt
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctaat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1681 aaaacataat cagctaataa cacctgtagc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacactg agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tgaaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DAVMMR010000112         2173 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMMR010000112 DAVMMR010000000
VERSION     DAVMMR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.112"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            160..1653
                     /locus_tag="VYS74_002012"
     CDS             160..1653
                     /locus_tag="VYS74_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3789990.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1666..>2173
                     /locus_tag="VYS74_002013"
     CDS             1666..>2173
                     /locus_tag="VYS74_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3789991.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLL"
BASE COUNT          764 a          329 c          435 g          645 t
ORIGIN      
        1 catataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tattcaggtg acgcatagat atagttaatt gaagctttgt ttgaaatctg ataaaataat
      121 gatattacta agttttaaaa actaaagaaa agggaagata tgattacagg cgaattaaaa
      181 aataaaatcg atcagctgtg ggaaattctt tggacagaag gaaacgcaaa tcctttaaca
      241 aatattgaac agttgactta tctcttattt atgaaagatt tggatagtgt cgagcttgga
      301 cgtgaaagtg atgctgaatt tctagggatt ccttatgagg gagtttttcc aaaagataaa
      361 cctgaatacc gttggtcaac ttttaaaaat ataggagatg ctcaggaagt ttatcgttta
      421 atgactcagg agatttttcc gtttattaaa aatctcaagg gggatacaga tgatacagcc
      481 ttttcacgat atatgcgaga agctattttt caaataaata aacctgctac gcttcaaaag
      541 gcaatttcta tcttagatgt ttttccaact aggggattag atgtagattt tgataatgac
      601 aaacaaagta ttactgatat cggagatatc tatgaatatc tgttatcaaa attgtcgacc
      661 gcaggtaaaa atggacagtt ccgtacacct cgtcacatca tcgatatgat ggttgagttg
      721 atgcaaccga ctatcaaaga tatcatctca gatcccgcta tgggttctgc tggcttctta
      781 gtatctgcta gccgttactt aaagcgtaag aaagatgaat gggaaaccaa tacagataat
      841 atcaatcatt ttcataatca gatgtttcat ggaaatgata cggatacgac tatgttgaga
      901 cttggggcga tgaacatgat gctacatgga gtagaaaatc cacaaatcag ttaccttgac
      961 tcgctgtctc aagataatga agaagccgat aaatatactt tggttttagc aaatcctcct
     1021 tttaagggct cacttgacta caattcaacc tctaatgacc ttcttgcaac cgtaaaaacc
     1081 aaaaaaacag aattactctt tctttctctt ttcttgcgaa ctttaaaacc aggtggacga
     1141 gcagcagtta tcgtacctga tggtgtcctt tttggttcgt ctaaagctca taaaggaatt
     1201 cgtcaggaaa ttgtagagaa tcataagctt gatgctgtaa tctcaatgcc tagtggtgtg
     1261 ttcaagcctt atgctggagt ttcaactgcc attctcatct ttacaaaaac tggtaatggt
     1321 ggtactgaca aagtctggtt ttacgatatg aaagcggatg gtttaagttt ggatgataag
     1381 cgacaaccga ttagcgacaa tgatattcca gatattatcg aacgctttca tcatcttgaa
     1441 aaagaagcag aacgtcagag aacggatcaa tctttctttg ttccagttgc tgagataaag
     1501 gaaaatgatt atgatttgtc tatcaataaa tataaagaga ttgagtatga aaaagttgag
     1561 tatgaaccaa cagaggtcat cttaaagaaa atcaatgatt tagaaaaaga aattcaagct
     1621 ggcttggctg aattagaaaa attactcaag tagggaggtg gctgtatgaa aaaagtgaag
     1681 ttgggggaag tcttatctct aaaaaaaggc aagaaagcca ctgtacttgc tgaacaaaca
     1741 actctaagcc aacgttatat tcaaatagat gatttaagaa ataataataa tttaaaattc
     1801 actgaaagtt taaatatgac tgaagcactc ccagatgata ttctgatagc atgggatgga
     1861 gctaatgcag gaacagttgg ttatggatta tcgggagctg ttggtagtac aattacggtc
     1921 ttaaaaaaga atgagcgata caaagaaaaa attatatcag attacttggg agtctttttg
     1981 gaaagtaaat cgcagtattt acgagaacat tcaacaggtg caacaattcc tcatttaaac
     2041 aagaatatat tacttgattt acaattagaa ttgctaggta tcgaagaaca agagaacatt
     2101 atctgtattc ttaatacgat taaagggctt attactaaaa gaaaattgca gttagatgaa
     2161 ctaaacttgc tcg
//
LOCUS       DAVMMR010000113         1878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMMR010000113 DAVMMR010000000
VERSION     DAVMMR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.113"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            319..1665
                     /gene="gdhA"
                     /locus_tag="VYS74_002014"
     CDS             319..1665
                     /gene="gdhA"
                     /locus_tag="VYS74_002014"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV3789992.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          593 a          347 c          357 g          581 t
ORIGIN      
        1 tgattttagt ctggtataaa tattgctttc ctccaaaatg gtcatagttt tactggcaaa
       61 tctaacatat cacggataaa ttaacaagtg atttctgaat tactaaacat tttcttttct
      121 tatagcatac catactttaa gattttgtct ttaagaaaga tatttccaag aaaaacgttc
      181 gtttttttat ctattttcaa aaaaaccaaa aactgaattg aaagaatttt tagaaaattt
      241 ctgttttttc tttacagtga aaaaaaattc tgctataatg tcttatgtaa taaaatatga
      301 caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt gtttgaaact
      361 gtgaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga atttttcaac
      421 actttagaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat cttggcacgt
      481 attactgagc ctgagcgcgt ggtttctttc cgtgttcctt gggttgaccg tgatggaaaa
      541 attcaagtaa accgtggtta ccgtgttcaa ttcaactcag ctgttggacc atacaaaggt
      601 ggacttcgtt tccacccaac tgtaaaccaa gggatcttga aattcctcgg atttgaacaa
      661 atctttaaaa atgtcttgac tggacttcct atcggtggag gtaaaggtgg atcagacttc
      721 aatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt catgactgaa
      781 ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg tgttggtgga
      841 cgtgagattg gttaccttta cggtcaatac aaacgtctta accaatttga tgctggtgtc
      901 ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc aactggttac
      961 ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt tgctggtaag
     1021 aaagtcgtta tttcaggttc tggtaacgtt gctcaatatg ctcttcaaaa agcaactgaa
     1081 ctcggtgcaa ctgttatttc tgtttctgac tcaaatggtt atgtcatcga tgaaaatggt
     1141 atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gtgctcgttt gactgagtat
     1201 gcagctgaaa aagcaactgc aacctaccac gaaggtactg tatggactta cgctggaaac
     1261 tatgacattg ctcttccatg tgccactcaa aacgaaatca acggtgaagc agctaaacgt
     1321 ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag cgaccttgat
     1381 gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgcaaaagc tgccaacgct
     1441 ggtggtgtag ccgtttcagc tcttgaaatg agccaaaaca gtcttcgcct ctcatggact
     1501 cgtgaagaag ttgatggccg tctcaaagac atcatgacaa acatcttcaa cacagctaaa
     1561 acgacttcag aaacatacgg tcttgataaa gactaccttg caggagctaa cattgctgcc
     1621 tttgaaaatg tagcaaatgc catgattgca caaggtattg tttaagattt atttactcaa
     1681 tcctcaatca ctacgattgg ggatttttag cttgaattaa aaaattggaa aatctaatta
     1741 aataaaatga aatgcaataa aaactgaaca aattgattgc ggaattcaaa ccaatttcta
     1801 acatatttct agaaattaat ttgacttttc taatcgattt gttcatatct tatttcaatt
     1861 tactatactt acttaacc
//
LOCUS       DAVMMR010000114         1858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMMR010000114 DAVMMR010000000
VERSION     DAVMMR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.114"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(3..968)
                     /locus_tag="VYS74_002015"
     CDS             complement(3..968)
                     /locus_tag="VYS74_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3789993.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VYS74_002016"
     CDS             complement(1086..1499)
                     /locus_tag="VYS74_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV3789994.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VYS74_002017"
     CDS             complement(1496..1732)
                     /locus_tag="VYS74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV3789995.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          541 a          362 c          275 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggatt
//
LOCUS       DAVMMR010000115         1491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMMR010000115 DAVMMR010000000
VERSION     DAVMMR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.115"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            143..1339
                     /gene="tuf"
                     /locus_tag="VYS74_002018"
     CDS             143..1339
                     /gene="tuf"
                     /locus_tag="VYS74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV3789996.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          464 a          315 c          303 g          409 t
ORIGIN      
        1 taaattttta ccaagaataa ttcacaaaaa cgttgtaaaa cacttgcaat ttagctgaaa
       61 tttgataaaa tagtaaggaa agttagactg tattgcctac tgtctatcta taaaatatat
      121 tttattggag gcttttactc aaatggcaaa agaaaaatac gatcgtagta aaccacacgt
      181 taacattggt actatcggac acgttgacca cggtaaaact accctaactg cagctatcac
      241 aactgttttg gcacgtcgct tgccttcatc agttaaccaa cctaaagact atgcgtctat
      301 cgatgctgct ccagaagaac gcgaacgcgg tatcactatc aacactgcgc acgttgagta
      361 cgaaactgaa aaacgtcact acgctcacat cgacgctcca ggacacgcgg actacgttaa
      421 aaacatgatc actggtgctg ctcaaatgga cggagctatc cttgtagtag cttcaactga
      481 cggaccaatg ccacaaactc gtgagcacat ccttctttca cgtcaggttg gtgttaaaca
      541 ccttatcgtc ttcatgaaca aagttgactt ggttgacgac gaagaattgc ttgaattggt
      601 tgaaatggaa atccgtgacc tattgtcaga atacgacttc ccaggtgacg atcttccagt
      661 tatccaaggt tcagcactta aagctcttga aggtgactct aaatacgaag acatcgttat
      721 ggaattgatg aacacagttg atgagtatat cccagaacca gaacgtgaca ctgacaaacc
      781 attgcttctt ccagtcgagg acgtattctc aatcactgga cgtggtacag ttgcttcagg
      841 acgtatcgac cgtggtatcg ttaaagtcaa cgacgaaatc gaaatcgttg gtatcaaaga
      901 agaaactcaa aaagcagttg ttactggtgt tgaaatgttc cgtaaacaac ttgacgaagg
      961 tcttgctgga gataacgtag gtgtccttct tcgtggtgtt caacgtgatg aaatcgaacg
     1021 tggacaagtt atcgctaaac caggttcaat caatccacac actaaattca aaggtgaagt
     1081 ctacatcctt actaaagaag aaggtggacg tcacactcca ttcttcaaca actaccgtcc
     1141 acaattctac ttccgtacta ctgacgttac aggttcaatc gaacttccag caggtactga
     1201 aatggtaatg cctggtgata acgtgacaat cgacgttgag ttgattcatc caatcgccgt
     1261 agaacaaggt actacattct ctatccgtga gggtggacgt actgttggtt caggtatggt
     1321 tacagaaatc gaagcttaat tcgatttagt tcccagaaga acaattattt aagtcagaca
     1381 ctaaaagaat cttgcttggc aaggttcttt tttggctctt tgtcaactgt agtgggttga
     1441 aaaaaaacta agctcgagaa aggacaaatt tcgtcctttc ttttttgatg t
//
LOCUS       DAVMMR010000116         1413 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMMR010000116 DAVMMR010000000
VERSION     DAVMMR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.116"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            159..1361
                     /gene="rpsA"
                     /locus_tag="VYS74_002019"
     CDS             159..1361
                     /gene="rpsA"
                     /locus_tag="VYS74_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV3789997.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT          448 a          222 c          326 g          417 t
ORIGIN      
        1 ttaggtaata atcaagataa aaagcaaaat atcttagggt attttatttt tataattgaa
       61 agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc
      121 aagtttaaga aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc
      181 taaatagcgt tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg
      241 atgcgactca agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc
      301 gcgaattgac aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat
      361 tggatgttct tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg
      421 tatctaaaaa acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag
      481 aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag
      541 gtgttcgtgg atttatccca gcttcaatgt tggatactcg tttcgtacgt aacgctgagc
      601 gttttgtagg tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct
      661 tcatcctttc acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat
      721 tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg
      781 gcgctttcat cgaccttggt ggtgttgacg gattggttca cttgactgaa ttgtcacatg
      841 aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc
      901 ttgatcttaa cgaagaagaa ggacgtgtat cactttcact taaagcaaca gtaccaggac
      961 catgggatgg cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac
     1021 gtttgactga cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg
     1081 tatcacaaat ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag
     1141 aagttcaagt taaagttctt gaagttaacg cagatgcaga acgcgtatca ctttctatta
     1201 aagctcttga agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc
     1261 gtccacgtcg tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag
     1321 gattttcaat ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa
     1381 aatcctttgt ttactaaaca agggattttt ctg
//
LOCUS       DAVMMR010000117         1164 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMMR010000117 DAVMMR010000000
VERSION     DAVMMR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.117"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            226..>1164
                     /gene="pspC"
                     /locus_tag="VYS74_002020"
     CDS             226..>1164
                     /gene="pspC"
                     /locus_tag="VYS74_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV3789998.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEREGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPSPDTKPSPQPEGKKPSVPDINQEKEKAKLAVVTYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQLEK"
BASE COUNT          459 a          199 c          227 g          279 t
ORIGIN      
        1 tccataatat ctatagtgga tttacccact acaaatatta tagaaccatt taatttctgt
       61 tgaattctag tttcattaga atagcagagg aagaaaaatt attgcaggaa gttattattc
      121 caaatggaag aataaaagga tagcgaaaat aatgtagcaa atttcatata caattcatat
      181 tagcaggata tactgtagtt aattaaaatt atggagaata tagacatgtt taaatcaaat
      241 catgagcgaa gaatgcgtta ttccattcgt aaatttagtg taggagtagc tagcgtagct
      301 gttgccagtc tttttatggg aagtgttgta catgcgacag agagagaggg aagtacccaa
      361 gcagccactt cttttaatag gggaaatgga agtcaggcag aacaacgtgg agaactcgat
      421 ttagaacgag ataaggcaat gaaagcggtc agtgaatatg taggaaaaat ggtgagagat
      481 gcctatgtaa aatcagatag aaaacgacat aaaaatactg tagctctagt taaccagttg
      541 ggaaacatta agaacaggta tttgaatgaa atagttcatt caacctcaaa aagccaacta
      601 caggaactga tgatgaagag tcaatcagaa gtagatgaag ctgtgtctaa atttgaaaag
      661 gactcatttt cttcgtcaag ttcaggatcc tccactaaac cagaaactcc gcagccggaa
      721 aatccagagc atcaaaaacc aacaactcca tctccggata ccaaaccaag ccctcaacca
      781 gaaggcaaga aaccaagcgt accagacatt aatcaggaaa aagaaaaagc taagcttgct
      841 gtagtaacct acatgagcaa gattttagat gatatacaaa aacatcatct gcagaaagaa
      901 aaacatcgtc agattgttgc tcttattaag gagcttgatg agcttaaaaa gcaagctctt
      961 tctgaaattg ataatgtaaa taccaaagta gaaattgaaa atacagtcca caagatattt
     1021 gcagacatgg atgcagttgt gactaaattc aaaaaaggct taactcagga cacaccaaaa
     1081 gaaccaggta acaaaaaacc atctgctcca aaaccaggta tgcaaccaag tcctcaacca
     1141 gaggttaaac cgcagctgga aaaa
//
LOCUS       DAVMMR010000118         1157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMMR010000118 DAVMMR010000000
VERSION     DAVMMR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.118"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            238..837
                     /locus_tag="VYS74_002021"
     CDS             238..837
                     /locus_tag="VYS74_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3789999.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            980..1150
                     /locus_tag="VYS74_002022"
                     /pseudo
     CDS             980..1150
                     /locus_tag="VYS74_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          411 a          172 c          234 g          340 t
ORIGIN      
        1 ttaatatttg gagaagagga tggccatctt ctccattttt atgtgcagag ttatagtgac
       61 ttgatgctgg gaaagtacac tgtgactgct aatactcaat gaaaatcaaa gtgcaaacta
      121 ggaagctagc cgcaggctgt acttgagtac gtcaaggtga agctgacgtg gtttgaattt
      181 gattttcgaa gagtataaaa cattcctaga agttactttg atttccctaa tctatttatg
      241 caaattttat gttattttac tataacagtt gttgctaagc caaataatag tggggaagtt
      301 catttagacg taagcattga agataatcag ggaggtagtg ggcataattt cagttctgtt
      361 tcaagtagct cacaaacagc taaatatgaa ggaactggtt ataataacaa ttcatcatta
      421 tatataacga ttgataaaac gtctgatgca acagctcttt tgaaattaaa gttgaataat
      481 gttgataatc aacctgctac tgaagttcct agttcaggaa ttactgtaaa attaaatgct
      541 aaagataatg ctggaaactg gacaagtgct tcgaataaaa aagaagtaac agtaaaaatt
      601 gtttctgcta aaccgacata tccagacaaa atcttagtga aaaatcctga taatataaaa
      661 gatacagaaa aaaatgccat tattgaaaaa ttgaaagagg caaataaaaa tcatccagca
      721 ggagctccaa cctttgctaa aggtgaagga gagcatgcaa atgatattgt agcaacttat
      781 tcagatggta caacttatta tgtaccgtta aatgatgtga caaaatatgc gaggtagtgg
      841 ctgtaccact cacttattca cctccccgtg atttgtagta gtgataggct ttctcactat
      901 tattataaaa caaaataaag atcacaacac tttttcattc tgtgttgtgc cttgagtgaa
      961 acgaaaggaa tgaattataa atatgaaaag tatagtcact agcatagatg agcgcttgcg
     1021 tactcgccta cgagtgatta tctggaagca atggaagaag aaatcgagac gattatgggg
     1081 attgcttaag ttaggggttc ctaaatggat agcagataag gtatctggct ggggcgacca
     1141 ctatcaatta tattaaa
//
LOCUS       DAVMMR010000119         1108 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMMR010000119 DAVMMR010000000
VERSION     DAVMMR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.119"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            231..706
                     /locus_tag="VYS74_002023"
                     /pseudo
     CDS             231..706
                     /locus_tag="VYS74_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            744..1046
                     /locus_tag="VYS74_002024"
     CDS             744..1046
                     /locus_tag="VYS74_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3790000.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
BASE COUNT          376 a          177 c          215 g          340 t
ORIGIN      
        1 gtgttttgag taacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
       61 gaacagaaaa atgcttggag tatttgtttg tgtgtttatt tttatataac aaactataaa
      121 cacaacaaaa atataaaaaa agagacaaaa aagaacagaa agtaattaac aacgatttca
      181 tatagtgtta tacttatagc ataaagttaa tggaaagaag ggaaaacctt atggtattag
      241 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaataactct
      301 ttgatcaggt agcaagcttg ttggaggaac gagaaattgt aactccaact tatcgtgaag
      361 ctttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt ctaggaaagg
      421 acttgcaaat gtaatgattc ctcacacaga tattgttcat aatctagctg agaaagtggt
      481 ggttgttcga ttagagaaac cggtgacttt ccataatatg attgcgccag gtaaggaagt
      541 agaagtatcc ttgctcttct ttatcatcaa taattcaagt tcaagtcaaa cgaacattct
      601 tgctcagttg atggactttt tcacaggaaa tggacatctt gaagacctat caaaaatttc
      661 cgaaccagaa aaactttatg cttatataga tgaagcaatc gcttaatctt gtctattaaa
      721 aaataaaatc ggaggaagtc tatatgatta aaattcttgc tgcctgcggt gcaggtgtta
      781 actcaagtca ccaaattaaa agtgctctag aagaagaact ttcaaaccgt ggttacgatg
      841 ttcactgtga tgcagttatg gtgaaagatg taaacgaaga ccttatgaaa ggttacgata
      901 tctttacacc aatcgctgca acagaccttg gttttgaacc aggtatccca gttatcgaag
      961 ctgggccaat cttattccgt atcccagcaa tgagcgttcc agtatttgac aatattagac
     1021 ttcctgcgaa acaaaatatg gtatagtagt tctatgaatg atgaagcaag taaacaacta
     1081 actgatgcac gatttaagcg tcttgttg
//
LOCUS       DAVMMR010000120         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMMR010000120 DAVMMR010000000
VERSION     DAVMMR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.120"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            145..1017
                     /locus_tag="VYS74_002025"
     CDS             145..1017
                     /locus_tag="VYS74_002025"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV3790001.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          305 a          190 c          244 g          356 t
ORIGIN      
        1 cacaaaaaaa tacaattgtc ttgacaattg tattgacact tgttagaatt aaaaatgagg
       61 tattcaggct tgtgatttcc tatggaagtt aatagtaatt gcctctagtg cttacaagtg
      121 atattaaaaa tagaggactt agtgatgtca atcatttcaa ctgatttaac cccttttcaa
      181 atagatgata cattgaaagc agccttgcga gaagatgttc attccgaaga ttacagtacc
      241 aatgccattt ttgatcatca tggccaagcc aaggtgtcgc tttttgccaa ggaagctggt
      301 gttttagcgg gactaaccgt ttttcaaagg gtttttaccc tatttgatgc cgaggtgacc
      361 ttccagaatc ctcatcaatt taaggatggg gatcgtttga ctagtggcga tttggtttta
      421 gaaatcatag gctcggtgag aagtctctta acatgtgaac gcgttgcctt gaatttttta
      481 caacatttat cagggattgc ttcgatgaca gctgcttatg tagaagcctt aggcgatgat
      541 tgcattaagg tatttgatac tcgaaaaact actcctaatt tacgtctttt tgagaaatat
      601 gccgtgagag ttggcggtgg ctataatcat cgctttaatt tatcagatgc tatcatgcta
      661 aaagacaatc acattgcggc agtaggtagt gttcagaagg caattgctca agcgcgtgcc
      721 tatgcccctt ttgtgaaaat ggtcgaggtg gaagtggaaa gccttgctgc tgccgaagaa
      781 gctgcggcgg cgggtgctga tattatcatg ttggataata tgtcattgga acagattgaa
      841 caggccatta ccctaattgc aggacgttct cggattgaat gttctggaaa tattgatatg
      901 accactatta gccgttttcg tggtttagcg attgattacg tctccagtgg tagtttaacc
      961 catagtgcta agagtcttga tttttccatg aagggtttaa cctaccttga tgtctaagtt
     1021 gtaaaataaa ctaacttttt aaaggatgtc tttcctctag aacgagtttt atgtcagata
     1081 gtttaaacgc ctctt
//
LOCUS       DAVMMR010000121         1050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMMR010000121 DAVMMR010000000
VERSION     DAVMMR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.121"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            72..527
                     /locus_tag="VYS74_002026"
     CDS             72..527
                     /locus_tag="VYS74_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3790002.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            692..991
                     /locus_tag="VYS74_002027"
     CDS             692..991
                     /locus_tag="VYS74_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV3790003.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          371 a          209 c          177 g          293 t
ORIGIN      
        1 gattatacca cattgtgtac tatttttggt tcatttcact ataataataa cgatactaga
       61 taatgaggaa aatgcaatgg caaacccaac tttcggagaa aaaagcgaat acagactata
      121 ttgctcgcta tggcgtatat gcagttatcc ctaatcctga acaaaaacaa attgttcttg
      181 ttcaagaacc aaatggtgct tggttcctac catgtggaga aattgaagca ggtgaaaatc
      241 atcaggaagc cctaaagcgt gagttgattg aagagcttgg tttcacagca gaaattggta
      301 cctattacgg acaagctgac gaatatttct attctcgtca tcgtgatacc tactactaca
      361 atcctgccta cctctatgaa gcaactcctt tcaaagaagt acaaaagcca ctagaaaact
      421 ttaatcatat tgcctggttc cctattgacg aggctatcaa aaaccttaaa cgtggtagcc
      481 ataaatgggc cattgaatct tggaaaaaac agcataagat tgactaatac tcaatgaaaa
      541 taaaattcaa accacgtcag cgtcgcctta ccgtactcaa gtatagcctg cggctagctt
      601 cctagtttgc tcttttattt tcattgagta taaatcaagc gactttattc aaaaagtgct
      661 ataatagaat tagaaaatcg gaggaaatat tatgaagctt atcaatacga caaactcaca
      721 ctcgcaactt gtaaaaagtc agctagaaag taccgatgca acccttgttg aggtttattc
      781 tgcagggaat acagatgtta tttttaccca agctccgctt cactatgaaa tcctcatctc
      841 aaacaaacac cgtgctattc gtgagcctga aatcgaaacc attcaagaat ttttcttgaa
      901 acgtaaaatt gataagacct ctgttgatga agccaatatc aagacactct actcagagaa
      961 attaattgga atttcaatcc caatcaagta agaaaattga gtaaaaactc gatttgagag
     1021 aaagtccaat aagtctttcc ataataaaac
//
LOCUS       DAVMMR010000122         1013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMMR010000122 DAVMMR010000000
VERSION     DAVMMR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.122"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(497..895)
                     /locus_tag="VYS74_002028"
     CDS             complement(497..895)
                     /locus_tag="VYS74_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3790004.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          299 a          197 c          153 g          364 t
ORIGIN      
        1 gacggctatc gattgtcaaa tgtttggctt ttgtagtata attgtcttgc atctctgtgc
       61 ctttcttgtg tttgtggttg aacaacaagt ataacacaga ggtgttttct tatgcctaca
      121 agagctttca ttatttccat tttcttttgg atttcactct attctgaaaa acttgtgtat
      181 atttactgaa gctagcaagt cttacctgta aatttaatga aagcaacaca aaatccgaga
      241 ggggaatctc ggattaatag atagagagtt tttagtttaa ataaattgtt taaaatatca
      301 acaacatcac ttcttttctt aacctgataa gtcttgattc ctaattttgg ggctacgatt
      361 atattgtcct caatatcgtc tagaaagaca caatttctag gttataactg gtatttatcg
      421 atagttactc atatacatca gtccacctcc atacttatgt gcgagcctct ctttgtatta
      481 tacctccata ctcaccttac agattctttt ggtaataata tctttgccta atgtagagat
      541 agtcttgcaa agaaaaaact tccttgtagc catgtttctg ataaaagtcc ggtgcctgga
      601 actggtaagt attgacaaag gcaaaacaac aatttcgatt cttagcttca ctttctgcct
      661 gttgcaatag ttttgaaccg attccttgcc ctcgcagttc ctcttttaca aacaaatact
      721 cgatttctag ccaatttcca aaagtctctg ctatcaaacc tgccaggaga ttgccctttt
      781 catcttcgac ataaagatta agtggctcac tttcagcctc ttctcttttt gaacggttat
      841 aaacacgaat cagattccct atttcttgcg atttatgtga ttccttattt tccaatctaa
      901 agtatagtga aatgaaataa aacatgcgca aatcgattaa ggaatttaat ctaatttcta
      961 acaatgtctt agaaatcaaa gtgtactatt ttaacttcaa tgcactatac atc
//
LOCUS       DAVMMR010000123          997 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMMR010000123 DAVMMR010000000
VERSION     DAVMMR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.123"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..>997)
                     /locus_tag="VYS74_002029"
     rRNA            complement(<1..>997)
                     /locus_tag="VYS74_002029"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          215 a          316 c          205 g          261 t
ORIGIN      
        1 agggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctacggcact aaaccccgga aagggtctaa cacctagcac
      181 tcatcgttta cagcgtggac taccagggta tctaatcctg tttgctcccc acgctttcga
      241 gcctcagcgt cagttacaag ccagagagcc gctttcgcca ccggtgttcc tccatatatc
      301 tacgcatttc accgctacac atggaattcc actctcccct cttgcactca agttaaacag
      361 tttccaaagc ctactatggt taagccacag cctttaactt cagacttatc taaccgcctg
      421 cgctcgcttt acgcccaata aatccggaca acgctcggga cctacgtatt accgcggctg
      481 ctggcacgta gttagccgtc cctttctggt aagataccgt cacagtgtga actttccact
      541 ctcacactcg ttcttctctt acaacagagc tttacgatcc gaaaaccttc ttcactcacg
      601 cggcgttgct cggtcagact tccgtccatt gccgaagatt ccctactgct gcctcccgta
      661 ggagtctggg ccgtgtctca gtcccagtgt ggccgatcac cctctcaggt cggctatgta
      721 tcgtcgcctt ggtgagccgt taccccacca actagctaat acaacgcagg tccatctggt
      781 agtgatgcaa gtgcaccttt taagcaaatg tcatgcaaca tccactctta tgcggtatta
      841 gctatcgttt ccaatagtta tcccccgcta ccaggcaggt tacctacgcg ttactcaccc
      901 gttcgcaact catccagaga agcaagctcc tccttcagcg ttctacttgc atgtattagg
      961 cacgccgcca gcgttcgtcc tgagccagga tcaaact
//
LOCUS       DAVMMR010000124          952 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMMR010000124 DAVMMR010000000
VERSION     DAVMMR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.124"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            105..584
                     /locus_tag="VYS74_002030"
     CDS             105..584
                     /locus_tag="VYS74_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3790005.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VYS74_002031"
     tRNA            678..749
                     /locus_tag="VYS74_002031"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          354 a          134 c          222 g          242 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggagcaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caagaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa aggctgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggacca cttgatgaaa
      781 aaaagtttaa aaaaacttaa aaatcttcaa aaaagtgttg acaagcgaaa gcagttgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaaca taagttgtag tactgaacaa tg
//
LOCUS       DAVMMR010000125          934 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMMR010000125 DAVMMR010000000
VERSION     DAVMMR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.125"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>934
                     /locus_tag="VYS74_002032"
     CDS             <1..>934
                     /locus_tag="VYS74_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3790006.1"
                     /translation="AEADALVDAETDALVLAEADALVDAEAEALVDAEADALVDAEAE
                     ALVEADSEALVDAEAEALVLAEADALVDAETDALVLAEADALVDADSDADVLAEAEAL
                     VDAEAEALVDAEADALVDAKADALVDADSDAEVLAEAEALVDAEAEALVLAEAEALVD
                     AEAEALVEAEAEALVDAEAEALVLAEAEALVDAEADALVDAEAEALVDAEAEALVDAE
                     AEALVLAEAEALVDADSEADVLAEADALIDAEADALVDAEAEALVDADSDALVLAEAE
                     ALVDAEAEALVEAEALVLAEAEALVDAEAEALVLA"
BASE COUNT          180 a          207 c          318 g          229 t
ORIGIN      
        1 gctgaagccg acgcactcgt tgatgctgag actgatgcgc tggtacttgc tgaggctgac
       61 gcactggttg acgctgaagc tgaggcgctt gtcgatgctg aagccgacgc actcgttgat
      121 gctgaagctg aggcactcgt tgaggccgat tcagaggcac tcgttgacgc tgaagctgag
      181 gcgctggtac ttgctgaagc tgacgcactc gttgatgctg agactgatgc gctggtactt
      241 gctgaggctg acgcactggt tgacgctgat tcagatgctg atgtgcttgc tgaggctgag
      301 gcactcgttg atgctgaagc tgaggcgctt gtcgacgcgg aagctgacgc actcgttgac
      361 gctaaagccg acgcacttgt tgatgccgat tcagacgctg aggtgcttgc tgaagctgag
      421 gcactcgttg atgcggaagc tgaggcgctg gtacttgctg aggctgaagc acttgtcgac
      481 gctgaagctg aagcgcttgt cgaggctgag gctgaagcac tggttgacgc tgaagctgag
      541 gcgctggtac ttgctgaggc tgaagcactc gttgatgcgg aagctgacgc acttgttgat
      601 gctgaagctg aagcacttgt tgatgctgaa gctgaggcac ttgtcgatgc tgaggctgag
      661 gcgctggtac ttgctgaggc tgaagcactt gttgatgcag attcagaagc tgatgtgctt
      721 gctgaagctg acgcacttat tgatgctgag gctgacgcac tggttgacgc tgaagctgag
      781 gcacttgttg atgctgactc agatgcactg gtacttgctg aagctgaagc actcgttgat
      841 gctgaagccg aggcgcttgt tgaggccgaa gcactggtac ttgcagaggc tgaggcgctt
      901 gttgatgcgg aagctgaggc gcttgtgctt gctg
//
LOCUS       DAVMMR010000126          926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMMR010000126 DAVMMR010000000
VERSION     DAVMMR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.126"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(74..147)
                     /locus_tag="VYS74_002033"
     tRNA            complement(74..147)
                     /locus_tag="VYS74_002033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VYS74_002034"
     tRNA            complement(157..242)
                     /locus_tag="VYS74_002034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VYS74_002035"
     tRNA            complement(250..321)
                     /locus_tag="VYS74_002035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VYS74_002036"
     tRNA            complement(344..416)
                     /locus_tag="VYS74_002036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..517)
                     /locus_tag="VYS74_002037"
     tRNA            complement(435..517)
                     /locus_tag="VYS74_002037"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Leu,seq:tag)
     gene            complement(523..595)
                     /locus_tag="VYS74_002038"
     tRNA            complement(523..595)
                     /locus_tag="VYS74_002038"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Lys,seq:ttt)
     gene            complement(620..692)
                     /locus_tag="VYS74_002039"
     tRNA            complement(620..692)
                     /locus_tag="VYS74_002039"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Asp,seq:gtc)
     gene            complement(695..767)
                     /locus_tag="VYS74_002040"
     tRNA            complement(695..767)
                     /locus_tag="VYS74_002040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Val,seq:tac)
     gene            complement(772..887)
                     /gene="rrf"
                     /locus_tag="VYS74_002041"
     rRNA            complement(772..887)
                     /gene="rrf"
                     /locus_tag="VYS74_002041"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          235 a          269 c          199 g          223 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact ttgaaccccc acgccgttaa gcgccagatc
      481 ctaaatctgg tgcgtctgcc aattccgcca aacccgcata tatgacccgt actgggctcg
      541 aaccagtgac ccattgatta aaagtcaatt gctctaccaa ctgagctaac gagtctaaaa
      601 taacttgcgt taccttaaac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca
      661 gggcgacgtg ataaccgcta cactacggga cctttgggag ttaacgggat cgaaccgctg
      721 accctctgct tgtaaggcag atgctctccc agctgagcta aactccctag agctaagcga
      781 cttccctatc tcacaggggg caacccccaa ctacttccgg cgttctaggg cttaacttct
      841 gtgttcggca tgggtacagg tgtatctcct aggctatcgt cacttaactc tgagtaatac
      901 ctactcaaaa ttgaatatct attcaa
//
LOCUS       DAVMMR010000127          858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMMR010000127 DAVMMR010000000
VERSION     DAVMMR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.127"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            296..760
                     /gene="queF"
                     /locus_tag="VYS74_002042"
     CDS             296..760
                     /gene="queF"
                     /locus_tag="VYS74_002042"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV3790007.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
BASE COUNT          263 a          196 c          161 g          238 t
ORIGIN      
        1 gtagattgaa actagaatag tacatctcta cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgat cgatttgtcc tgttcttatt tcactttact atataaatag aatattacga
      121 tatttgggat atgccatcgc taaaaaaaac ctcgatttgg tattttttag ctccctaatg
      181 ggagcttttt acgtgctctg aacatttccc attttggaag gagtactatg aaacgtcaat
      241 cagccttggt cgtcttagtg gtggtcaaga ttcaaaaacc tgccttttct ggaccatgca
      301 acactatgaa acagtcgaag ctgtcacctt tgcctacggc caacgtcatc acctcgaaat
      361 tcaaattact agagaaatcg ctaaggaaca gggcattcgt caccatatcc tcgatatgtc
      421 tctgctggga caaatcactg ctcagccaga ctttgcgacg attcatattt cctacattcc
      481 tgacaagctc tgtgtcgagt caaaatccct caaactatat ctatttagct accgaaacca
      541 cggagatttc cacgaaaact gtatcaacac catcgggaaa gacttggtca acttgctaga
      601 ccctcgctat ttagaagtct ggggaaaatt cactccgcgc ggtggcattt caatcgaccc
      661 ctactataac tacggtaagc aaggaactaa gtatgagggc ttggcagaac aacgcctctt
      721 ccaacacgac ctttatccag agaaaattga caaccgctaa agtcatactc aatgaaaatc
      781 aaagagcaaa ctaggaagtt agccgcaggt tactcaaagc agtgtttgag gttgtagata
      841 aaactgacga agtcagct
//
LOCUS       DAVMMR010000128          815 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMMR010000128 DAVMMR010000000
VERSION     DAVMMR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.128"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            111..815
                     /locus_tag="VYS74_002043"
                     /pseudo
     CDS             111..815
                     /locus_tag="VYS74_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          285 a          152 c          168 g          210 t
ORIGIN      
        1 caagaaaagt gcaaataaga aatctccaga ttaggaacta tccgtgagtt ctctagtctg
       61 gagatttttc aatagacttc ctgcgaaaca aaatatggta taatagttct atgaattatg
      121 aagcaagtaa acaactaacc gatgcacgat ttaagcgtct tgttggtgtt caacgcacga
      181 cttttaaaga gatgttagct gtattaaaaa cagcttatca acttaaacac gcaaaaggtg
      241 gacgaaaacc taaattaagc ctagaagacc ttcttatggc cactcttcaa tatgtgcgag
      301 aatatcgcac ttatgaacaa attgcggctg tttttggtat tcacgaaagc aacttaatcc
      361 gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc
      421 ctctcagctc tgaggacacg gtaatgattg atgcgacgga agtacaaatc aatcgcccta
      481 aaaaaagaat tagcgaatga ttctggtaaa aagaaatgcc acgctatgaa ggctcaagcg
      541 attgtcacaa gtcaaaggag aattgtttct ttggatatca ctgtgaacta ttgtcatgat
      601 atgaagttgt tcaaaatgag tcgcagaaat atcggacaag ctggtaaaat cttggctgac
      661 agtggttatc aagggctcat gaagatatat cctcaagcac aaactccacg taaatccagc
      721 aaactcaagc cgctaacagc tgaagataaa gcctgtaatc atgcgctatc caaggagaga
      781 agcaaggttg agaacatctt tgccaaagta aaaac
//
LOCUS       DAVMMR010000129          718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMMR010000129 DAVMMR010000000
VERSION     DAVMMR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.129"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>718
                     /locus_tag="VYS74_002044"
     CDS             <1..>718
                     /locus_tag="VYS74_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3790008.1"
                     /translation="YTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTIHTEI
                     KRRTVRQCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQKFSP
                     EMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQSIEQ
                     RPEAINLRLENGHYEIDTVLLTRSKNYCLLVLTDRKSRHQIIRLIPNKSAEVVNQALK
                     LILKQHKILSITADNGTEFNR"
BASE COUNT          253 a          145 c          147 g          173 t
ORIGIN      
        1 attatactac aaaagccaaa catttgacaa tcgatagccg tcgcttaatc gaaagatgga
       61 aaaaagaagg aaaatcaaat agagaaattg cctctctact tggaaaagct cctcaaacta
      121 tccacactga aatcaagcgt aggacagtcc gacaatgtct tggaaaaggg cgcttcaaag
      181 aggtttattc tgccgactat gctcaacagt cttatgaaaa taatcgcaag cactcggtca
      241 agagatcaag cgtgaccaag gaactaaagg aaaagattct ccactatcat aaccaaaaat
      301 tttcgcctga aatgatggtt atggctaaag gggttaacgt gggaatttca accatttact
      361 attggattca tcatggaaaa ttggggttaa gcaagcagga tctgctttac cctagaaaag
      421 gaaaagcgct taagaaacag gctagcacca actttaaacc tgctggtcaa tccatcgaac
      481 agcggcctga agctatcaat cttcgcttgg agaatgggca ttatgagatt gatacggttc
      541 tacttacgag atcgaaaaac tactgcttgc ttgtcttgac ggatcgaaag agtagacatc
      601 agatcatccg attgattcca aataaaagtg ctgaggtggt caatcaggct ctaaaactca
      661 tcttaaaaca acacaagatt ctttccatca cggcagataa tggaacggaa ttcaatcg
//
LOCUS       DAVMMR010000130          699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMMR010000130 DAVMMR010000000
VERSION     DAVMMR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.130"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>699
                     /gene="phtB"
                     /locus_tag="VYS74_002045"
     CDS             <1..>699
                     /gene="phtB"
                     /locus_tag="VYS74_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV3790009.1"
                     /translation="HQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVEDILAFLAP
                     IRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTH
                     SHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVP
                     LDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYY
                     VEHPNERPHSDNGF"
BASE COUNT          222 a          139 c          155 g          183 t
ORIGIN      
        1 ttcaccaaga tttacttgat aataaaggtc gacaagttga ttttgaggct ttggataacc
       61 tgttggaacg actcaaggat gtctcaagtg ataaagtcaa gttagtggaa gatattcttg
      121 ccttcttagc tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct
      181 acactgatga tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt
      241 atatctttga tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata
      301 tgacccatag ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc
      361 aggcttatgc taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa
      421 atactgaggc aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc
      481 cacttgatcg tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa
      541 tcatacctca ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggccttt
      601 atgaggcacc taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg
      661 aacatccaaa cgaacgtccg cattcagata atggttttg
//
LOCUS       DAVMMR010000131          694 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMMR010000131 DAVMMR010000000
VERSION     DAVMMR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.131"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(271..441)
                     /locus_tag="VYS74_002046"
     CDS             complement(271..441)
                     /locus_tag="VYS74_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3790010.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(454..>694)
                     /locus_tag="VYS74_002047"
     CDS             complement(454..>694)
                     /locus_tag="VYS74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV3790011.1"
                     /translation="SIVILDEATSALDTINEERITKYIKSQGCTQIIVAHRLSTIKDA
                     DIIFVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
BASE COUNT          231 a          137 c           84 g          242 t
ORIGIN      
        1 aaagagcaaa ctaggaagcg agccgcaggc tgtacttgat tacggcaagg caaagctgac
       61 gtggtttgaa gagattttca aagagtatta aacaaaaaga aaaaccgcta ctcctaagaa
      121 tagcgattta atcatgtttt taagctacta ctgtagttgc tctattattt aagtgttatg
      181 aataacttca atatctttaa agctttttgt tagatttata atttcaacta tagccatctc
      241 tactccttag tttacagcaa aaaattatgc ttaattctta ttgtttttgt aaacaatcag
      301 cgaacccaat actattccat tcaacaagcc taaagaaata ccatattcaa ctggaaaatc
      361 taaaaaaatt gcaaacacta ttccaatcat cacccctagt gaggctgttg ttaaaattac
      421 atattcattt tctttcttca ttttctttac acctcatttc ctttttgtat ataagctgta
      481 gtactctcca ccaagatcca ttaagtactt atgatttcct gactcaacaa tcttaccacc
      541 tttcattaca aaaataatat ccgcatcctt aatcgttgac aatctatgag ctacaattat
      601 ttgagtacag ccctgacttt ttatatactt tgttattctt tcctcattaa tagtgtctaa
      661 agcactagtt gcttcatcta aaattacaat acta
//
LOCUS       DAVMMR010000132          653 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMMR010000132 DAVMMR010000000
VERSION     DAVMMR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.132"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            121..>653
                     /locus_tag="VYS74_002048"
     CDS             121..>653
                     /locus_tag="VYS74_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV3790012.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGH"
BASE COUNT          242 a          112 c          138 g          161 t
ORIGIN      
        1 gtttagctga aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta
       61 taagaaaaca cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag
      121 atgcaagaac attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt
      181 gagcggtgga agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaaaggcg
      241 cctcaaacga ttcataatga agtcaaaaga ggtacaactt tacaacaagt gagaaaaggg
      301 ctatacaaaa aggtctattc tgccgattac gcacaaactg tttaccaatt caatcgaaaa
      361 cggtcggtga aaaagttaat tttaacaaag gaaatcagag agaagatctt acactatcat
      421 aagcaaaaat tttcgcctga aatgatggtt aacaagaagc aagtgaaagt tggtatttca
      481 accatctact actggtttca taatggtcat ttaggattga cgaaggccga catgctttat
      541 cccagaaaaa ggaaaggtgt caagaagcaa gctagtccga actttaagcc ggcaggtaaa
      601 tctatcgaag aacgtcctga cgttattaat cttcgcttgg aaaatggtca tta
//
LOCUS       DAVMMR010000133          570 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMMR010000133 DAVMMR010000000
VERSION     DAVMMR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.133"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..>570)
                     /locus_tag="VYS74_002049"
     CDS             complement(<1..>570)
                     /locus_tag="VYS74_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3790013.1"
                     /translation="IRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNI
                     ITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHI
                     VQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLT
                     NKEILNKLLSYSQDLKHHYQLYQLLLFHFQ"
BASE COUNT          184 a           97 c          117 g          172 t
ORIGIN      
        1 ctgaaagtga aacagcaaga gttgatagag ctgatagtga tgtttcaagt cttgtgaata
       61 gctcaaaagc ttgtttaaaa tctctttatt ggttaagtgc atacgaaaag taggacgata
      121 aaatcgctta tcactcagtt tacggctatc ctgttgaatg agtttccagt agcgcttgat
      181 agccttgtat tcatgggatt ttcgatgaaa ctgattcatg atttggacac gcacacgact
      241 catagcacgg ctaagatgtt gtacaatgtg aaagcgatcc aacacgattt tagcacacgg
      301 gaaaagttgt ctagctaaat catagtaagg actaaacata tccatagtaa tgattttgac
      361 gcgacatcgg acggctctat catatttaag aaagtgattt cggatgacag cttgtgttct
      421 tcccttaaga acagtgatta tattgagatt gttaaaatct tgcgcaatga agctcatctt
      481 tccttttgta aaagcatact catcccaaga cataatctca ggaagacaag aaaaatcatg
      541 tttaaagtgg aagtcattga gctttcgaat
//
LOCUS       DAVMMR010000134          529 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMMR010000134 DAVMMR010000000
VERSION     DAVMMR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.134"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(1..529)
                     /locus_tag="VYS74_002050"
                     /pseudo
     CDS             complement(1..529)
                     /locus_tag="VYS74_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238800.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          165 a          111 c          107 g          146 t
ORIGIN      
        1 ggttaaatgc atacgaaaag tagggcgata aaaatgttta tcgctgagtt tacgactatc
       61 ctgttgtatg agcttccagt agcgcttgat agccttgtat tcatgagact ttcgatccaa
      121 ttgattcatg atttgaacac gcacacgact catagcacgg ctaagatgtt gtacaatgtg
      181 aaagcgatcc aacacgattt tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc
      241 ctgagcctag aaatttgaaa gcgaagctgt ttagccaagt catagtaagg actaaacata
      301 tccatagtaa taattttgac gcgacatcgg acaactctat cgtagcgaag aaagtgattt
      361 cgaatgatag cttgtgttct accctcaaga acagtgataa tatcgagctt ttcaaaatct
      421 tgcgcaatga agctcatctc catctcccga ttgaaacagt cactccccgg actgtttcaa
      481 cctcccaaga cataatctca ggcagatttc taaaattaca ctcaaagtg
//
LOCUS       DAVMMR010000135          445 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMMR010000135 DAVMMR010000000
VERSION     DAVMMR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.135"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>445
                     /locus_tag="VYS74_002051"
     CDS             <1..>445
                     /locus_tag="VYS74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3790014.1"
                     /translation="NKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKNVTK
                     NGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGM
                     VILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILL"
BASE COUNT          179 a           57 c           71 g          138 t
ORIGIN      
        1 aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga taggggcaaa
       61 aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt aaatacaaag
      121 aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa aacaaaggat
      181 aaattacttc gaaaaggcaa acttgagcgt tatgatatag tcttgacaac aagaggtact
      241 gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg tataaattca
      301 ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat tatccatgtt
      361 ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca gttaccaatt
      421 acaaaattaa aaaaaatact tctcc
//
LOCUS       DAVMMR010000136          430 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMMR010000136 DAVMMR010000000
VERSION     DAVMMR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.136"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>430
                     /locus_tag="VYS74_002052"
     CDS             <1..>430
                     /locus_tag="VYS74_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3790015.1"
                     /translation="PLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIA
                     WKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELM
                     KVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADF"
BASE COUNT          154 a           63 c           74 g          139 t
ORIGIN      
        1 cctttaaata ataacaagaa atttgcagta aaaacgggac aacagtgttt taaattttca
       61 agtggaaaat ttcttgataa acatgataga gtttttgaag gttatcctgc ctatggtgga
      121 aatggcattg cttggaaaag tcgaaaatat ttaattgata atcctactat catcattggt
      181 cgtgtaggtg catattgtgg taatgtaaga actacccacg gtaaggtatg gataagtgac
      241 aatgctatat atataaaaga atttaagaat tccgatttca atcttgtatt tttattagaa
      301 ttaatgaaag taattgattt tagtaaattt gctgatttct caggtcaacc aaagataact
      361 caaaaaccat tagaaaatca gaaatatatc ctcccccctc tagccctcca aaatgagttc
      421 gcagactttg
//
LOCUS       DAVMMR010000137          376 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMMR010000137 DAVMMR010000000
VERSION     DAVMMR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.137"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(188..275)
                     /locus_tag="VYS74_002053"
     tRNA            complement(188..275)
                     /locus_tag="VYS74_002053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Ser,seq:gct)
     gene            complement(282..355)
                     /locus_tag="VYS74_002054"
     tRNA            complement(282..355)
                     /locus_tag="VYS74_002054"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Ile,seq:gat)
BASE COUNT          122 a           92 c           65 g           97 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttc atcgctctga acatcaaaaa agaaaggacg
       61 aaatttgtcc tttctcgagc ttagcttttc ttcaacccac tacagttgac aaagagcctt
      121 aagtttacgg atctgaagtt ttattgtctc taaaaacaaa aaagagagtt acaaactctc
      181 attaaaacgg agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag
      241 caaaccgtcc tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga
      301 accaccgacc tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt
      361 aaaaaacttg gtaatt
//
LOCUS       DAVMMR010000138          367 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMMR010000138 DAVMMR010000000
VERSION     DAVMMR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.138"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>367
                     /locus_tag="VYS74_002055"
     CDS             <1..>367
                     /locus_tag="VYS74_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3790016.1"
                     /translation="SRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWV
                     PDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVS
                     RYIPAKDLSAETAAGIDSKL"
BASE COUNT          115 a           91 c           72 g           89 t
ORIGIN      
        1 caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa ccattaccac tttatccctt
       61 atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt cgttatcgtt
      121 caaaccattg ggtaccagat tcaagaccag aacaaccaag tccacaatcg actccggaac
      181 ctagtccaag tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg
      241 agaaattggt caaagaagct gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg
      301 gagtttctcg ttatatccca gccaaggatc tttcagcaga aacagcagca ggcattgata
      361 gcaaact
//
LOCUS       DAVMMR010000139          366 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMMR010000139 DAVMMR010000000
VERSION     DAVMMR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.139"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>366
                     /locus_tag="VYS74_002056"
     CDS             <1..>366
                     /locus_tag="VYS74_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3790017.1"
                     /translation="LLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITAD
                     NGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKTTPKEVAFIE
                     NWINNYPKKCLDYKSPSEFL"
BASE COUNT          121 a           71 c           74 g          100 t
ORIGIN      
        1 ctgttagtct taaccgaccg gcggagcaga caccaaatta taaggttaat tccaaataaa
       61 actgctgaat ctgtcaatca ggcgcttacg ttactattag gggagcatcg tattctgtcc
      121 attactgcag ataatggttc ggagttcaaa cgattgtctg aggtatttcc tgaggaacat
      181 atctactacg cacatgctta ctcttcatgg gagagaggtt caaatgaaaa tcataatcga
      241 ttaattcgga gatggttacc taaaggaacc aagaaaacga ctccgaaaga agtagctttt
      301 atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg actacaagtc gccaagtgaa
      361 tttctt
//
LOCUS       DAVMMR010000140          299 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMMR010000140 DAVMMR010000000
VERSION     DAVMMR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.140"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(169..>299)
                     /locus_tag="VYS74_002057"
     CDS             complement(169..>299)
                     /locus_tag="VYS74_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3790018.1"
                     /translation="AMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT           90 a           54 c           53 g          102 t
ORIGIN      
        1 aaaaaactcc gtagtctcta gcgctataaa tccggctaga gattacagag ttatttttta
       61 atctaaactt cctacaaggg tatattatct actgaaagga tatggtattt gtagggaata
      121 ctttaaagag ttcttacagg aagtcagtat taactagtta tattaagttt agtttaccca
      181 ttcaccattg gcattgactc tatagctatc tacagttgtg ttgactgcaa gggcacctga
      241 gccattgaca tagtaccatt tatctgatac tttgaaccat tggcttgctt tcatagcac
//
LOCUS       DAVMMR010000141          243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMMR010000141 DAVMMR010000000
VERSION     DAVMMR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.141"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..>243)
                     /locus_tag="VYS74_002058"
     CDS             complement(<1..>243)
                     /locus_tag="VYS74_002058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3790019.1"
                     /translation="AEALVLAEADALVEAEAEALVEAEAEALVDADSDAEVLVDAEAE
                     ADVDADSDALVLAEAEALVEAEAEALVLAEADALVDA"
BASE COUNT           58 a           82 c           55 g           48 t
ORIGIN      
        1 agcgtcaacc agtgcgtcgg cttcagcaag tacaagcgcc tcagcttcag cctcaacgag
       61 tgcctcagct tcagcaagta caagtgcgtc tgagtcagca tcaacgtctg cctcagcctc
      121 agcgtcaaca agtacctcag cgtctgaatc agcatcaacc agtgcctcag cttcagcctc
      181 aacgagtgcc tcagcttcag cctcaacgag tgcgtcggct tcagcaagta ccagtgcctc
      241 agc
//
LOCUS       DAVMMR010000142          235 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMMR010000142 DAVMMR010000000
VERSION     DAVMMR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.142"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            complement(<1..>235)
                     /locus_tag="VYS74_002059"
     CDS             complement(<1..>235)
                     /locus_tag="VYS74_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HEV3790020.1"
                     /translation="DAEADALVEADSDAEVLAEAEALVEADSDAEVLAEAEALVDAEA
                     EALVDADSEALVDADSDADVLAEADALVDADSDA"
BASE COUNT           60 a           77 c           51 g           47 t
ORIGIN      
        1 cagcatctga atcagcgtca acgagtgcgt cagcctcagc aagtacatca gcatctgaat
       61 cagcgtcaac cagtgcctct gaatccgcat caacgagtgc ttcagcttca gcatcaacaa
      121 gcgcctcagc ttcagcaagt acctcagcgt ctgaatcggc ctcaacaagt gcctcagctt
      181 cagcaagcac ctcagcgtct gaatcggcct caacaagtgc gtcggcttca gcgtc
//
LOCUS       DAVMMR010000143          215 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMMR010000143 DAVMMR010000000
VERSION     DAVMMR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.143"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            <1..>215
                     /locus_tag="VYS74_002060"
     CDS             <1..>215
                     /locus_tag="VYS74_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3790021.1"
                     /translation="QPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMA
                     TGWLQNNGSWYYLNANGAMATGWLQYN"
BASE COUNT           73 a           58 c           46 g           38 t
ORIGIN      
        1 gcaaccgcca aaagcagaaa aaccagctcc agctcctgca ccaaaaccag agcaaccagc
       61 tcccgcacca aaaacgggct ggaaacaaga aaacggtatg tggtacttct acaatactga
      121 tggttcaatg gctacaggtt ggctacaaaa caacggttca tggtactacc tcaacgctaa
      181 tggtgctatg gctacaggat ggctccaata caatg
//
LOCUS       DAVMMR010000144          205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain Serogroup 19 isolate IPD
            SAMEA4889526-rid17917553.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMMR010000144 DAVMMR010000000
VERSION     DAVMMR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4889526
            Sequence Read Archive: ERR2852124
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001718929.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001718929.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/04/2023 18:29:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 48 of 116
            Pseudo Genes (internal stop)      :: 33 of 116
            Pseudo Genes (multiple problems)  :: 21 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4889526-rid17917553.denovo.144"
                     /strain="Serogroup 19"
                     /serovar="19A"
                     /isolate="IPD"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia"
                     /collection_date="2008"
                     /collected_by="Pneumococcal Reference Laboratory, NSW,
                     Australia"
     gene            78..151
                     /locus_tag="VYS74_002061"
     tRNA            78..151
                     /locus_tag="VYS74_002061"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:112..114,aa:Met,seq:cat)
BASE COUNT           48 a           49 c           60 g           48 t
ORIGIN      
        1 aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca
       61 tcctcctttt atattaacgc gggatggagc agctcggtag ctcgtcgggc tcataacccg
      121 aaggtcgtag gttcaaatcc tgctcccgca ataaggctcg gtagctcagt tggtagagca
      181 atggattgaa gctccatgtg tcggc
//
