LOCUS       DAWAAE010000001        37454 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_461_length_37454_cov_3.113479, whole genome shotgun sequence.
ACCESSION   DAWAAE010000001 DAWAAE010000000
VERSION     DAWAAE010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 37454)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 37454)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..37454
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_37454_cov_3.113479"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1809..1818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4949..4958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8390..8399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10482..10491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12285..12294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12573..12582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13746..13755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19534..19543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22474..22483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27477..27486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7156 a        11538 c        11479 g         7181 t
ORIGIN      
        1 ccccagcgtc tgtcgtcccg gccctatgca ttccgaacgt cactccgaaa aaacctcccg
       61 gagcgatttg gcaagaatat gcagtccctt ttccacagta tcacgctcgc ccggcgtcat
      121 gcgctcaaag gcgcgcagct ctatcttgcg catttccccg atgacttcgt cggcaaaagc
      181 ctgtccttcc ggcgtgaaag cgatcaggtt gccgcgaaag tcctccggat ccttccggcg
      241 ggtgatgaac ccccgcgcct ccagctgctt gagcgaggcg gagatcgtct gcttcggata
      301 gctcagcgtt tcgcagagat acttctgcgc acatctctcc ggtgcggtat agatgcgctc
      361 aagaatgaac agcgtcgtat ccttgatatc gtagcgggcg caccatttgt agtacaccgc
      421 gtcaaagcaa aagtaatcct cataataatg cttgatgcat tccagcagcc ggtcccgtga
      481 ttccatagat cctccataca tttttggact atattcacaa tattataata aagcccaaaa
      541 tgtgtcaagt caggaatttg ttaatattgt cgagctgtag atttaacttc atatctttat
      601 tcggaatgtt tttatcgttt ttcgataatt ttatattgac tttcagattt ctgtatgata
      661 ttctccgctc atggaggcga tagcatgaaa caaaaactta cctcgccggg cgtcgcccgg
      721 ttcaccaaag ctcttctgga cattctcttt tatgccggca tcgccgtaac ggtctctctg
      781 ccgtggacga tgcgatgggc ggcgcggttt tatccctcgt tcgcggcgca gctcacgctg
      841 cacacgacgc tcttcctgct cgccggggtg tgcgcggtgc tgatcctctt cgagctgcgg
      901 cggatgctca aaaccgttgt ctccggcgac tgcttcgtgc ggcagaacgt ttcaagcctg
      961 tgccgcatgg cgtactatgc ctttgcgatc gcggcgctca tggcggtgcg gctcatcgtg
     1021 ctcttcacgc cggggatgct cgccatcgtg tgcgtgtttc tgctggcggg tctcttctcg
     1081 ctcgtgctcg cgcaggtgtt cgaccgcgcc gtgagctaca agcaggacaa cgatctcacg
     1141 atctgaggtg gaagcatgat cgtagtaaat ctcgatgtaa tgatggcaaa acggaagatc
     1201 gggttgggcg agctggccga gaaggttgac ataaccatgg caaatctctc cgttttgaag
     1261 aataacaagg cccgcgccgt gcgcttttcc acgctggacg cgctctgccg ggcgctggac
     1321 tgtcagccgg gcgacattct cgcctgggaa ccggacaagg aggaatacga tggattctaa
     1381 ttcggaatcg gtgtggtacg gcgcgccccc cgcgccggag actatgagcg cgccggagcc
     1441 gcagcacgag gacgaacgcc atgttcgtct gcggacggac gcccggtatt ttctgctgct
     1501 ctcgcttgcg ctgggcgcgg tgttcgcgct tcttttcgtg aatgcctacg cgctcgggct
     1561 caacgcggcg ctgctcgccg cggcgatggc ggcggcaacg cttctcgcgc tcggcaagct
     1621 cggcctgcgg gagagaaaac gcgatatttt ctggtcggtc tctctggttt tgctcggcct
     1681 ttcgattgca tggaccgcaa accatatgac gcagaacgtg agctttctcg gtatgttcat
     1741 cgccgagctg ctttggctga tgaacgcctt tgcggatatt ctctcgtggc gcttcgggcg
     1801 cgtcgtttnn nnnnnnnncg tcgtttccgg cggcttccgg ctcgtcggac ggatcatcac
     1861 gcacctggga gagccgttcc gccatctcgc cgcgctgcgc cggggcagta agcaaaccac
     1921 gcggagcgtg ttgctcggca ttgtgatcgc cgtgccgctt ctctggatcg tcgtatcgct
     1981 gcttgcgagc gcggacgccg cgttcggcgc gctgatcgag cgcatattcg gcgtctggag
     2041 cgtttccaaa accgttcgta cggtgatccg cgctgttttc tatacgctga tccccgccct
     2101 ggtcttctac agcgcgctcg ccgcgcagac cgagcgcagc gactttccgg caaaggaaaa
     2161 acgccgcgcg agcgccctcg tcgccataac gttcaccggc gcgctggccg tcgtttacgc
     2221 cgtgttctgc ggcatccaga tcgccgtgct gttcgccggg gacgtttccg cgctgccgga
     2281 gggcatgacg tatgccgagt acgcccgcga gggctttttc cagctgctgc tcgtaagcgg
     2341 catcaacgtc gtcctcatca tcacggcgca gcgccggttc gtatcgagca aagcgctgcg
     2401 ggcgctgctc gtatttctca ctgtgtgtac ctacctcatg gaggcatcga gcgcgatgcg
     2461 catgatgctc tatgtaaacg cctatggcct cacctatctc cggctgctgg tgctgtggtt
     2521 tctttcgctg ctcgcgctga tcctcggcgc ggcggtgtac acggtgttcc acgaggattt
     2581 ccggctgttc cgctttacgc tcattgcgtc catggcgctc tggctcgtgt tcgccttcgc
     2641 gcggccggac gcgatcgcgg cgcggtataa cctagcaaag cacggtctcg acgataccac
     2701 ggaggcagag atcgcttatg aatgctccag cgacgccatc ggcgcgctgc gcccctattt
     2761 tgcggatatc gccgggcacg gtgaggattc gacaacgtac ctcaacctaa cgatctccgt
     2821 gatgcgggag tattccgagc gcggggtacg cggcttcaat ttctccgtat gggaggccat
     2881 gcggacagcg gaggatttta tcgatgcata cacctgacgg cattctcatg taccctgtgg
     2941 cgcatatccg ctcggatttc ccgacgaagt tcggcattcc caagcagagc gggctttccc
     3001 aggcgctcga agcgcgggta gtattctgcc cggaataccg cagcgcggac gccgtgcgcg
     3061 ggttggagga attttcccat atctggcttc tctggcagtt ttccgccgcg gtgcgcgagg
     3121 gcgagacgtt ctcccctacc gtgcgcccgc cgcggctcgg cggcaacaag cgcgtcggcg
     3181 tgtttgcgag ccgcagcccc ttccgtccga accacatcgg tctctcctgc gtgaagctcg
     3241 agcggatcga atatacaggc gaagggccgg tgctcatcgt gcgcggcgct gatcttatgg
     3301 acggaacgcc cattctcgac gtgaagccct atcttcccta tgccgacagc cgcccggacg
     3361 cggccggcgg atttaccgac gccatttccg cgcgggtgct ccatgcggaa attccggaag
     3421 agctgctgcg gcgtgtgccg gaggaaaagc gcgaaccgct gcgcgaggtt ttggagcagg
     3481 acccgcgtcc gtcgtatcag gacgacccgg agcgcgttta cggcttcggc ttcgtcggta
     3541 tggaggtgcg cttccgcgcc gccgacggcg tgctgaccgt gctggacatt cagaaagagg
     3601 aataaaaaga ccccgccccg caaggtgaaa ttctcccctt tgtccggcag cacggcaaac
     3661 gaaacggcgc tctcccgcgt ctgcctatgg gaatgatctc cgccgcagag cgcagcgcaa
     3721 atgcggcata ccacaaataa aattttgcga ggaaagtcgg tttggatcca ttaccggaag
     3781 cacgggtttg cagaaatcgg ggaaatgtgc ggcattggga tcgctgccat atatgatata
     3841 tgaattgtaa ggagaggtac ggcatggata taagagcctt ctggcgggcg gttctcgcgc
     3901 aggacgagcc ggagatccga gcatttttcg ccccggacgc ctatgtgaac tggcgctgca
     3961 cgaacgagca tttcacggtg gatgaattca tcaccgccaa ctgcgagtat cccggagaat
     4021 gggacggggt cgtggaacgg atcgatacgc tgggcggcct taccgttacg gtcacgcacg
     4081 tttatccgaa ggatcggagc tgctcgttcc atgtgacatc cttccttcgg acggaaaacg
     4141 acaggatcgt atcgctggat gaatactggg ccgacgacgg cagcgcgccg caatggagat
     4201 tggataaaca catcgggact ccgatagaac gcatcggaga acaaaatgga cattgaactc
     4261 tattatcggg aggccggaag cggcgagccg ctgatcttgc tgcacgggaa tgacgagagc
     4321 ggcgaatact tcataaacca gatcgagcac ttccaaagcc ggtaccgcgt gatcgcgccg
     4381 gacacccgcg gccacggccg gtctccgaga ggagaagcgc ccttcaccat tgcgcagttt
     4441 gccaatgacc tttatgattt catgcagggg ctcggcatcg cccgtgcgat gatcctcggc
     4501 ttttcggacg gcgcgaacat cgcaatgaaa tttacgatgc agcaccccga gatggtaaag
     4561 gcgctgatcc tgaacggcgg caatctcgat ccctcgggcg taaagcgggc ggcgcagttt
     4621 cccatcgaga tcggatataa gattgccagc cactttgccg ccggatcgcc gggcgcaaag
     4681 aaaaacgccg aaatgctcga cctgatggta aacgatccga acatctcgcc ttcggagctt
     4741 gcaaagctca cgatgccgac gctggtggtc tgcggaacaa aggacatgat caaagagtcc
     4801 catacgagga tgatcgcaga aaacatccca aacgcctggt tggtgatcct tcccggagat
     4861 catttcgtcg ccaacaggca cccggcggaa ttcaatcagg tcgtagacga ttttctggaa
     4921 agcgttggat ccatatgaac gacaccccnn nnnnnnnngt gtgacgacga agcccttgcc
     4981 cgtccattcg acgctcaccg tcccgccgtc ggtgcggaat acctccgtgc cggcggcggc
     5041 gagcgctgcg aggagcttat ccccgatctc ctccgccgga gagaccgttg ccgcggcata
     5101 gcgcggcgtt gtctgcgcga taagctttaa aagcggctta ttgctgtcgc cgtggtgcgg
     5161 aagcttgatg agctcatagc tgtcgagcgc ggcgtcgaga aactcgttca ggcggttttt
     5221 cttcgcgtcg ccgaggagga ggaagctcgt ttcacgccat gtgacggtcg tgacgaggga
     5281 attgttgttg tcgtcgccgt agtcgatgtc gggcgggtcg agcgtaaacg agccgtttgc
     5341 cgtctcaacc gcatagttct ccgtcagtcg gaggaacggc gtattccgtt cttccgcggc
     5401 gcgcttgagc agcgtgagat acatggaatt ttcttcgtag tccgggccga ggatctgccc
     5461 caccggataa gcgcgcacga gcgccgccgc ggagccgatg tgatccttgt cgtaatgact
     5521 gaggatgaga tagtcgatgc gctgcacgcc gcggcgctca agcgcggcgc agatggagtc
     5581 aaaatcgtcc tcatcggcag cgtcgttcac gaccgtcacg ccgtccatgc atacgatgat
     5641 gcagtcggat ttgccggtat cgagaatgtc cagagagaat gtgccgccgt ccgtttccgg
     5701 cgcggcagcg ccgcacgcgc agagcgagag cagcgcgcaa agaaccaaaa gtgcggcaag
     5761 gcatcgtttc atgggatgta tctcctcttt cttcggcggg aatcacggtt tactttttcc
     5821 tctgccggaa gtataatcaa aataatagaa cgtatttcgg aggaaacacc atggagcttg
     5881 tcgtaaaaca ctattccgag ctgacggcgg acgagctgta cgacattctg cacgtgcgcg
     5941 tgctggtctt tgtcgtggag cagaaatgcc cctacgagga ggtggacggg tgcgacaggc
     6001 aggcgtacca tgtgtttctg cgcgacgaga ccggcatcaa ggcgtatctc cgcgtgctcg
     6061 accggggcgt ttcgtttgag gaggtgtcca tcggccgcgt gctgacgacc gagcgccgcc
     6121 ggccacaaga tcctcgacgc gggcatcgcc gcggcgcggg aaaagtacgg cgcggagacc
     6181 atccgcatcg aggcgcagac ctacgcccgg aagctctacg aggatcacgg tttccggcag
     6241 gacggcgagg aatttctgga ggacgatatc ccccacatcc cgatgatctg gcacgcgccg
     6301 aaagactgat ccatgatgta taacgtatcc cctgaagtgc tgtacagcgg ctcttcaggg
     6361 gatattttta tttcgccggt tcgaccgcat tttcctcgta caggcgcgga tagcgcgcac
     6421 ggtcgcagaa aatgagccaa acggacagca aaatcgagat cgtgagcgcg ccgatcgtca
     6481 gcccccaggc tgccgtcgag ccgtaagcgt catatgcctt gccttcggca atgttgaaaa
     6541 cgccgcgcag gatggtatac gccacgccgg atacgctgtt gatgcgtccg cggtgggagg
     6601 ccggggtgcg cgcggagaga tacggcccgt cggcaatggt attgatgatc tcgccccagg
     6661 tgaaaatgag catggcggcg taatacatcg ggatcgtgcc gaggaacgtg aggaacagga
     6721 tatatcccgc cagaaacagc agctttcccg tcaggagctt tttcgtatcg atcacgcggc
     6781 ggaaaaggcg tgtaatgaac ggggtaaaca aaacgaccac gatgcagttg agactcgaga
     6841 ccgtaccgta gatcaccgcg ccgttctcgc cgtggatgcg cccgagatcc agcggcatga
     6901 gatagccgta ctggtcgtat gccgcgctgc acagcgccga cacggcaaga tacagaagga
     6961 tcgtcggatt ctggcggagg atcgcaaaaa tgctctcccc gtctctcgcg cgctgatact
     7021 ccgcgcggct gccgatctgt tcgaccggcg tgatatcccg cacgaaaaac cagatgagga
     7081 tcgtcgagca gccgatggcg acgccgctga tgatgaacgc gagccacaga tagtttttga
     7141 acagcagacc gctgagcgtc ggcgaaagga caagaccgag attcgcgccc cagtacagga
     7201 gcgagtatgc cttatcacgc tcttcgaggg acgtgatatc ggcaatgatg gcgttgtacg
     7261 ccggaccctc aatggtctgg aaaaggctgg cgacaaacat gagcacgatc gtccccatgc
     7321 cgagcggaac gcatccacaa aggatgtagc acacgaccga aacgatgtcg ccgacaatga
     7381 tgatgttctt cttgttgagc cggtcggcga gcttgccgcc gaaaagcgtg gccggcatca
     7441 tcaaaatgcc gaacaccgcc gtaagtatgg caatgttccc ggcgctcata ccgagcttct
     7501 ggttgaggat catcgtgagc atgggccaga ccatcgagcc catgttcgtc acgatccgcc
     7561 cgaagaagag aacatagttc tcccggcgga gtccccggta ctgtgaaaag aagtgcatgg
     7621 atacttttcc tcgtattact cccgtatttt ctctctttct ttggttccta tgccgctatt
     7681 tgctcaaaag ccgcgcgacg aacgagcgct gcgcgcgcat cgcccccgag gggcagaact
     7741 cctggcagca gaagcaccgg acgcatttct tccggtcgat cacggcgtgc ttgttcacga
     7801 tcgtgatcgc gcccgccgga cagagccgcg cgcagtggcc gcagccggta cagccctcgc
     7861 cgagcgccgg tttggaccgg agcagcacgg cgacgcattt ttttacgagc ttccggaacg
     7921 gcttatcgtt cgggttcgac acaaaccagc tgcttgtggc gccggagcgg gcaaagtcgt
     7981 ttacatggta gggggcggcg tccttcacgc cgagcggctc gcccgcctcg gagaggagcc
     8041 ggcgctgctt tgccgccgtg agatacggca gctccttttc ctccagccca agcatccacg
     8101 cgcacagccg gtcgagctca taggagctcg tcgcggcgag gagcgcgccc atgtgccggg
     8161 gcgttccctg tgtggggccg ttgccctcca tgatgtccac ggcgtcacac aggcagagcg
     8221 ccgggcggac aaattcattg agatcaacga gcatgttggc aaacgccatc ggatcggggt
     8281 agcggaaatg gtactcactc ttcaccgtgc cggggatgac gccgtaaaga ttctttaccg
     8341 ccgctgtcat gcccatgagc ccgtgagcct tgagcttgga gaagttgatn nnnnnnnnng
     8401 atgacggcgt cgcatttttc gagccacgcg ctgtattcaa agctcttgac cgtgacggcg
     8461 tccggaaatt cgaccgtgtg atgcgaaaag tcacggttga gctcgccgcc ggcggcttcg
     8521 cagagcgcgt aatcgccgag cgagtacacg cggctcagca ccacggaggt gaacggctca
     8581 ccggggctgt cgccgagaac gacctccgcg ccccgctcgc gcagaaggcg cgtgagctcc
     8641 gcggccatga ccggatgggt cgtcgccgcc tgctccggct ttctcgccgc gcagagattg
     8701 agctttatgc cgatgcgcat gccgggcttc acccagtcaa gcccgccgac agcgtcaagc
     8761 gccgcaagga gcgcggcgcg caccgtttcg ggtgtataat ccccgcaggg gacgatggat
     8821 agtttcggtt ccggcatggt atgcctccca tatctacaga ctaattttgt attttaccac
     8881 atttctccct tccgcgcaac gggatatgca gcgatccgga atgtgtaaaa aatctgtcat
     8941 tgcacgaaaa acaggttgat aacccaacag ttatatgata tgcttactga gatagtattt
     9001 ttctgcgcga aaaagtcatt taatcaacaa aaaggggaac aggcgttatg ttatttgatg
     9061 gagatttcac actgaccgat cagatcgtgg acaaccacga gtgcaagagg gaaaatctga
     9121 tcggcatgat gcaggacgtg caggagcatt acaagtacct gccgccggag gtgctggtgc
     9181 atctggcggg acggctgcac atcagtccgg gcaaggtata cagcgtggcg acattctatg
     9241 accgcttctc gctggaagcc aaaggcaagt acattctgcg cgtatgcgac ggcaccgcct
     9301 gccatgtgcg caaatcgcag ccgatcctgg aggctctgga agagcatctc gggctgaacg
     9361 agaaaaaggt gacgagcagc gacatgctct tcacgctgga gaccgttgcg tgcctcggcg
     9421 cgtgcggcct tgcgccggtc ctcaccgtga acggcgaggt ccacgcgaaa atgacgccgg
     9481 agaaggcgat cgcgctcgtg gagagtatcc ggaaggagga gacgaaatga tccgacgtac
     9541 aaagctcacg tttgaagccc agcgctggaa ggcggagaaa gcgctcgcgc gcgagggcgt
     9601 ttcgatcatg gtatgcgccg gtacgggctg catcgccggc ggctcgctcg aagtttacga
     9661 ggagctgcgc cgcatctgcg cggagaaggg tctcgccgtc tccgtgaact tctccgaaca
     9721 tgcggacgac gccatccatc tcaagaagac cggctgccac ggcttctgtg agatcggccc
     9781 gctcgtcaac atcatgccgc aggacatttt ctatacccac gtcaagccct ccgactgcgc
     9841 cgagatcata gaaaagacga tcgtgaacgg gcagatcatc aagcgcctcc tctacaagat
     9901 gaacggcaag agctatgcca cgcaggagga agtccccttc tataataagc agcaccgcat
     9961 cgtgctcgag cgctgcggcc atatggacgc cgagagcatc tgggaatata tcgcccgcgg
    10021 cggctacaag gcgtttgaaa aggcgctctt cgatcttacc gacgagcaga tctgcattga
    10081 aatgctcacc tccggtctgc gcggacgcgg cggcggcggc ttccccaccg gcaagaagtg
    10141 ggacagcgtg cgccgtcagc cgagcggcga gaagtacgtc gtctgcaacg gcgacgaggg
    10201 cgaccccggc gcgttcatgg accgctccat catggagggc aatccacact ccgtcatcga
    10261 gggcatgatg atcgcggcga tcgccgtcgg cgcgcagaag ggatatatct acgtccgcgc
    10321 agagtacccg ctcgcggtcg agcggctgca gaccgccatc gatcaggcgc gcgatgtggg
    10381 acttctcggc gagaacatcc tcggcacgga attttccttt gatatccgca tcaaccgcgg
    10441 cgcgggcgac agccgctttg tcatgatggt ggatctcatc tnnnnnnnnn ngcgcgggcg
    10501 cgttcgtctg cggcgagggc agcgcgctga ccgcgtccat cgagggcaac cgcggcatgc
    10561 cgcgcaccaa gcccccgcgc accgtggaaa agggtctctg ggcgaagccc acggtgctca
    10621 ataacgttga gaccttcgcc gccgtaccgc tcatcatcga gcacggcgcg gcctggttcc
    10681 ggcaggtcgg caccgccggc tctcccggca cgaaggcgtt cgcgctcacc ggcaacgtgg
    10741 taaataccgg ccttatcgaa gtgcccatgg gcacgacgct tcgcgagatc atctttgaca
    10801 tcggcggcgg catcaaggac gggaagaagt tcaaggccgt gcagatcggc gggccgagcg
    10861 gcggctgtct cacggaggag catcttgatc tcccgctcga ctatgactcg ctcaagcacg
    10921 tcggcgcgat catgggttcc ggcggtctcg tcgtcatgga cgaggacacc tgcatggccg
    10981 aggtcgcccg attctttatg aaattcacgc agaaggaatc ctgcggcaag tgcatcccct
    11041 gccgcgaggg tacgaagcgc atgctggaag ctctcgagcg catcgtcggc aacgagggaa
    11101 gcgaagagga tatcgagctt ctcgaggatc tctccgacac gatctcccac accgcgctct
    11161 gcggtctggg tcagagcgcc tgcaagcccg tgcagtccac gctgcgctac ttccgcgaag
    11221 actacgagcg ccatgtgcgc gacaaattct gcccgctctg caacggcaaa ccaaaggtat
    11281 tgcagatcga tccggaaaag tgcaagggat gcagcctgtg cgcgcgcaac tgcccggtgg
    11341 aggcgatcac cggcgaggtc aagaagccgt tcgtgatcga cacggaaaag tgcatccgct
    11401 gcaacgcctg cgtcaagaac tgcaagttcg gcgcgattcg ggaggtgtaa agatatggca
    11461 aagggaatca tgtatatcaa cggccagcgc gtcgcgttcg acggggagaa aaacgttctc
    11521 gccgttatcc gcaaggcggg catcaatatg ccgacgctgt gctactactc cgatctctcc
    11581 gtttacggcg cgtgccgtat gtgcgtcgtg gaggacgata ggggtcgcat cgacgcctcc
    11641 tgctccatgg agccgcgcga cgggctctcc atccagacga acaccgcccg gctgctcaaa
    11701 taccgccgga tgattatcga acttttgctc gcctcgcacg accgtgactg caccgtttgc
    11761 gaaaagtcca gcgcgtgcga gctgcggcgg ctggcgctgc gtttcggcgt gcgccgcgtc
    11821 cggtttgagg atacgcgcga gcagctcccg ctcgatacct ccagcccctc ggtcgtgcgc
    11881 gacccgaaca agtgcatcct ctgcggcgac tgcgtgcgca cgtgcgagga gatccagggt
    11941 atgaacatca tcgactttac gcaccgcagc gccgatctgc gcgtaatgcc ggcattcgac
    12001 cagaagctcg ccgagaccga ctgcatcggc tgcggccagt gcgccgccgt gtgccccacc
    12061 gccgcgatca ccgtgtataa cgacatcggc cgtgtatggc gcgcgctgca cgacaagaat
    12121 aagcgcgttg tgttccagat cgctccggcg gtgcgcgtcg ccatcggcga agcgttcggg
    12181 ctgcatccgg gcgaaaatgc catgggcaaa atggtcgcgg cgctccggct catgggcgcg
    12241 gccgaggtgt ttgatacctc gttcgccgcc gccctcacca cggtnnnnnn nnnnatgttt
    12301 acctcctgct gcccggcatg ggtcaagtat gtggagtatc agcgccccga gcttctccgc
    12361 cacatctcct cctgcaagtc cccgatggag atgttcgccg ccgtgctgcg cgatcactat
    12421 gagaaaaaca gcgacggccg cgaggtctac cacgtcgccg tcatgccctg caccgcgaag
    12481 aagggcgagg cgaagcgcga ggagttcacc cacaacggca agcccgacgt ggacgccgtg
    12541 ctcacgacga tcgagctcat caacatgata aannnnnnnn nnacgaggcc gtggacatgc
    12601 cgttcggcat cacgaccggc tccggcgtca tcttcggcgc gaccggcggc gtggcggaag
    12661 cggtcgcgcg ctgctgctgc ccggacaaaa cgaaaaacgc ggcgatggcc atcgaatact
    12721 ccggtcttcg cggcgtggaa ggcgtgcgcg tggcaagcat gcttgtcggc gagcggacga
    12781 tccgcatcgc ggtgtgcaac ggccttctca acgcccagaa gctcatcgac gagatcgaga
    12841 ccggacgcga atactttgac ctcatcgagg tcatgacctg ccgcgccggc tgcgtcggcg
    12901 gcgcgggtca gccgcacggc cttacaaagg acaagtacga ccgcgccgcg ggcctctaca
    12961 acgccgacaa gactgccctc atcaagcgca gcgagcagaa ccccgtcatt gaaacgctgc
    13021 tgcgcgacat cggcgagcgt gagcacgagc tgctccacgt ccactacaag ggccgcgatt
    13081 tctgaaaagc ttaaagcttt caacgcaaaa cgtccggagg acattctccg gacgttttgc
    13141 attttttctt aaaatcagag ccggtcgttg ccgacagggg tgagaaagag ctttcggaac
    13201 tcctcgtcgt cggcggagtt tgccagcgcc cattcgacct tggcgacgac ggcctccgtc
    13261 gtcatcgtat aggcttcgag cagctcatac tttttcttga tgcggaagcc tacgcggtaa
    13321 agctccatat cgctcccctc gtgggagacc tgcgtggtga aaatgacctt cacgtggttc
    13381 cgaagcaggt ggccgatctt ctcggtgaac tcgtcgttat cgtagtacgg cacaccgccg
    13441 acgccgaaac tctcgatgac aacggcgtgg gcgtgctcgg ctacatagtc gagaatgccg
    13501 ggcgccatgc cggggatgag cttgatgacg caaacgcggc tgtcgagcgc atcataaaac
    13561 cgcaccggac cggggaacga ctcgcggata taatagcgca cctcgccgcc gcggatgaca
    13621 gcgacctccg ggtaatcgat gctcgaaaat gcgttgaagc tgcgcgtacg cgttttctgc
    13681 gcgcggttgg cgcagatgac cttattatcg aacacgagat gaatgccgca ggcgtcgtca
    13741 tcggcnnnnn nnnnngcata ggtgatcgca ttgcgcagat tttcccgcgc gtcggtatcg
    13801 cgcgcataga tggaaacctg cgagccggtc agcacgacgg gcttcgggct gttctggatg
    13861 agataataga gcgttgcggc ggcataggcc atcgtgtcgg tgccgtgggt gatgacaaag
    13921 ccgtcgtagc tttcatactt gtcccggatg atcttcacga gctcgaccca attctgcggg
    13981 cgcatgttcg tgctgtccag attaaagggc tgcaccgtgt cgatctcgca gagagagcgt
    14041 acctcgggga tgcattgcag cagctcctcg ctcgtcattt cgggcgtcag gccgtcctcc
    14101 gccgggcgcg aggcgatcgt gcctccggtg gcgatcagca gcagtttttt tctcatgcgt
    14161 tttcctcctt catcagctcg cgcagcagcg ccgccgcttc ctctccggat gcgccgacag
    14221 ccgcgcagaa ctgcgcggcg tcggtgagct tttcttttaa aatggcgcgg cgcatggccg
    14281 ctttgtccgt ctcgccgagc gcggcggcaa acgtcccgcg cccgacggcg gtgtggataa
    14341 agccgtcccg ctccagttct tcccaggcgc gccgcaccga aataagactt accgagagct
    14401 cctgcgccac ggtccgtatc ggcggcagca cctcgccggg cgctatctcc ccgcgcagga
    14461 tctgcgcggc aagctggtca tacatctgcc ggtagagcgg cagcggcgat gtattcgtca
    14521 ctgtcagctt catagatcca ccttttcaaa ccgctcggca ctgatctttg ccgagagcag
    14581 cgtaaaggcc gcccagagag cgatcccggc aaacagcacc ggaagctggc ggagctgtgc
    14641 gcttttctcc agcgtgtcaa gatacgcgct gaccggcgaa gggatgtact gtgcgagcga
    14701 ttccgcggca atgatataga gaaacgtaaa cacgccgccc cagaggaatg ccttgccctc
    14761 gctccgcccg gttttataga acaccgggaa gaaaatgagg ttgaagccgc cgtacatcag
    14821 aaacgacagg ccgaaaaacg cgacgttcgg ctcgatgccg gcgttattgc ctcccgcggg
    14881 attgatcttt gcagaaatta ccgcaaacgg gatggcgatg aggatgttgg caagctccag
    14941 cagcgcaccg gtaaggacgc gggcgcgcac cacgtcgccc ttgcgcaccg gcaggagcgc
    15001 ggaatagaaa atgtcgttct cggcgcggtt ggttttgaac gtgaggaata tgccgagcgt
    15061 cagatagaaa aagctgacat aatagggata cgacggcaca agcagcagcg ccgcgagcgc
    15121 gaagaagatg tatacgctcg gatggatggc gagccggaac tccttgtaca gcagattttt
    15181 cattctctcc cctcccgttc gatgtgcacc atgatgtcct ccatgccgat gggctccgcc
    15241 gcgagaccgg aagccattgc gtcctccgtc ttcatcatcg cttcaaaccc gaagctctgc
    15301 tcccgcagac cgatggcgct ctcctttacg gcgtccgtca gctcctcctt gccgccggtc
    15361 accttgcggt aagcgtcggt gaaggtgtcg cggtcggtgc ttgtgaggat ctttccgttg
    15421 tggatgtagg tgataaggtc ggcgcacttt tcgagatcgg atataatatg cgtggagaac
    15481 aaaatgctgc gctccccgtt cgcgaccgtg tcgcggaaaa tgtccagaag ctcgtcgcgg
    15541 ctcaccgggt caaggccgct tgtcggctcg tcgagaagga ggagccgcgc cccgtgcgag
    15601 agggcaagcg ccagcgtgaa cttgacccac atgccggtgg acagctcgcg cacgcgcttc
    15661 gtctcgtcga gcgagaagcg cttcatgtat tcgcggtacg cggcgtcgtc ccaatgctca
    15721 taaaaccgcg ccgtcgtctc cgccacatcg cgcaggcgct ttgagccgta atagcgcgcg
    15781 tcgcccagca ccacgccgat ctgctggcgg caggccgatt tgttttcccg gagatccata
    15841 ccgaatatct ccaccgtgcc gccgtccgga tgaacaaggc cgagcatgct tttgagcgtc
    15901 gtcgtctttc ccgcgccgtt gcgcccgatg aaccccatga tatacccgct ctccaaggag
    15961 aacgagacat gatccagcgt aaatcccgga tacgttttgc aaagatcgcg cacggacaaa
    16021 agctccatcg cgcattcctc cctgtcggat gttttattgt gtatgttcat tatacacaat
    16081 tcaatctatc tgtcaagccc cccgccttgc ccgatgggaa ataatctggt atagtatttg
    16141 cgatctttca aggagaaaca acgcaagtga gtaaaaatac gaaattatgg acggcgggca
    16201 cgctcaaaag ccgcggctgg acggaggagc tgatcgcctc tctcctgccg aagccgcagc
    16261 gtctttatcg gaacggccgc cgcgtgcgcg cctggcgcac agacgatatc cgccgcgccg
    16321 agcagagcga tagcttccgc tcgctgcgcg cggcgtctgc cgaaaatacg gaaagcatac
    16381 cggatgatgc ggcgctcgat gcagcgtcgc agcttctgaa aagcgcatgg gatgccgccg
    16441 cactgccgga aagccgtgaa gcgacgctcg cggcgcgcta ccatgaagcg atcctccgcc
    16501 agatcccggc ggtcggattt gccaagcggc tgcggccggg gcagagcatc agctatatcg
    16561 agcactttct ccatctggag accggcaccg gcgccgagcc gctcggcgca acgctccgcc
    16621 ggttcgtgac cgccgcgcca tggctcggcg cgaacgcagc ttctccgctc gccgggaagc
    16681 tctccgcgcg ctatgccgcg gtgctgcttg cgatcagcgc gcgggacatt gcttctttta
    16741 ccgccgcgca gccggaggcg aacatggacg ggcttctcga aaatggcgcc tttcccgtgc
    16801 aggagctttt gcagcacccg ctctcctatc tttattcggt cttttatatc ccgcgcgcga
    16861 tccgctcgag cctgaagctg ctcgtggcgc tcaacccgaa ggacgagtac ccggaagcgc
    16921 gcgccatgca gcgccatttt attctccata ccggcgggac gaacaccggc aagacctacg
    16981 ccgggtttga gcggctgcgc aaagccaaaa cgggcgtata tcttgctccg ctccgtctgc
    17041 tggcgcttga agcgcaggag cggctgcttg atctcggcgt tgcgtgctcg ctcacgaccg
    17101 gcgaggaaga ggaccgccgc gagggggata cccacgtcgc cgccacggcg gaaaagcttg
    17161 atctctccgc gcggtacgat gcggcggtga tcgacgagtg ccagatgatc gccgaccggg
    17221 aacgcggctt tgcctggacg cgcgcgatcc tcggcgtgct cgcgccggag gtgcatctct
    17281 gcgccgcgcc cgaggcgcgc gatcttctga tccgcatcat tgagagcacc ggcgacacgt
    17341 gggaggagat ccgtcatctg cgcaaaacgc cgctcgtgtg catgcgccgg gaaattgatt
    17401 ttgacgacgt aaagcccggc gacgcgctca tcacgttctc gaagctcggg gtgctttccg
    17461 tggccgagga tctgcgtgcg cacggaaagg aacccgccat catctacggc gcgctcccct
    17521 attccacgcg gcggcggcag atggagggct ttctcaaagg gcggatgcag tatgtcgttt
    17581 cgaccgacgc catcggcatg gggctgaatc tccccatccg gcgcgtgatc tttctcgaca
    17641 cgcggaagtt tgacggcgtg gagcggcgcg acttaaagcc cgccgagatc cagcagatcg
    17701 ccggacgcgc cgggcgctac ggcatgtacg acaagggcta cgtcggcgca acggagaatc
    17761 tggacttcat ccgcgagggg ctggaagcgg ccgtgccgcc gctcgaaacg gcggtggccg
    17821 ggttttccga gcttgtattg caggtggagc acgatcttct tgaggtgctg accgagtgga
    17881 acaaaatgcc gaccgtcgcg ccgtatgtaa agctcgatgt cagccggtat atcacgctca
    17941 tcggcaaggt gcggcaggag ggcttttcgc tcacgcgcga gcaggagctg cggtgcgcga
    18001 acattccgtt cgacgagacg gaggaagagc ttctggagct gttcttctcg tttctgcgct
    18061 gcttccggga gggtcagccg ctctcctgcc cggagcttcc cgagggacat gcgcctacgc
    18121 tcccgcagct cgagcagtac tgccggaagc tcgatctcta tttctcgttt tccaaggcgt
    18181 tcggctgccc gatcgaccgc gacgcgctct atgataagcg cgaggacgcc gccgagagca
    18241 tcaaccagat cctgctctac aatcttaaaa acaacatccg cttctgcgcg cgctgcggcg
    18301 cggcgctccc gctccaccag agcggacgcc tctgccagag ctgctaccgg aagatcagcg
    18361 ccctgcgccg cccgacgcgg tggtaaaaaa tcggccgtgc ctcccaattt ggggaggcac
    18421 ggccttttct ttggtttatt cggcttttaa gccgtattct tcggcgaggg cggcaaaggc
    18481 gggcagtgag cggcgcagcc gctcctccgg cacgcagtag gcaaggcggg cgtagcccgg
    18541 acaggcgaaa tcgtcgcccg gcacgagcag aagatcgtgc gccatcgcct tttggcagaa
    18601 ggcgcgtgcg tcctcctccg gtgattttac gaagagatag aacgccccct gcggcttcgc
    18661 gcaggaaaag ccgagcgcgg cgagtccctc gcagatgaga tcgcggtttt tcttatactc
    18721 gcgcagctcc cccgtctggc cgaggcacgc ggcgcacacg cgctgaaaga gcaccggcgc
    18781 gcagacatac cccagcatcc ggcccgcgcc ggaaatggcg gcgcgcagcg cggcgcggtc
    18841 tttgctctcc ggcggcacgg cgagatagcc gacgcgctcg ccgggcagcg agagcgattt
    18901 gctgaacgaa tagcagtaga tcgtgtcagg ataaaaaccg gggatgtacg gcacggcgtc
    18961 gccgtcgtag acaagatcgc ggtacggctc gtccgcgagg atgtagatcg ggtgaccgta
    19021 ctccgccgag cgctcggtga gcagagcggc gatcttcttt atgctctcct cccccagcac
    19081 gacgccggag gggttattgg gagagttcag gatcacgagc cacgtttttc cgttcaccgc
    19141 cgcggcgaag gcctcataat cgagctggaa cgtctccgac tcggagggca cctcgcacat
    19201 gacgccgccg gcggcctcgg tataggtgcg gtattcggaa aaatacggcg tgagcgtgat
    19261 gcactcgtct cccggcgaga gcagcgcacg gcagccgatg gcaaggcccg cggacgcgcc
    19321 gctcgtcatg tagatatcct ccggcgcgta ggtcatggaa aacgcgccgt tcagatactc
    19381 cgccacggcc ctgcgcacct ccggcaggcc ctgatcgggg ctgtaggcgt gcagctgcgt
    19441 gggggacatt tccttcataa ggcgctgcgc cgtctcctgc accgccgcgg gcggggcgat
    19501 gctcggcgag ccgatgctga aatcgtaaac attnnnnnnn nnncctcttc ggcgcggcgc
    19561 atggcgtaat cggcgatctc ccggatgggg ttgccggacg cgccgagcgc gtatacttcg
    19621 gatgggatca tgtggttttc ccccggttca caagatattc gaccgctctc cggtagccct
    19681 caaagcccag cccggcgaac gtcttttcgc acgccatgcc cgcgtaggac acgtgccgga
    19741 agctctcgcg ctcttgaata tcggtgagat gcacctccac cgccgggagc gccacggcct
    19801 tgagcgcgtc gagaatggcg acgctcgtgt gcgtatacgc cgcgggattg atgacgatgc
    19861 cgtccatccg tccgagggcg gactggatgg catcgacgat cgcgccctcg gggttggact
    19921 ggaaaagctc gcattctgcg ccgagctccc ggcaggacgc gcggatatat gcctcgagcg
    19981 cggcaaagtc acgccttccg taaagctccg gttcccgcac gccgagaagg ttcagattgg
    20041 ggccgttgat gacgaggatc ttcataggat caccttgcgg atgtcctcca cggtgtcctc
    20101 gagcgcgcgg ttgttgtcga tgatatagtc cgcaaagcgg agatagcgct tctcgcgcat
    20161 ttcgtacatg gcgttgagat ttcccgcgca ggagagcggg cggccgtcgc gctcgagctt
    20221 gtcaagatcg cggcgcagcc agaaaatgac gccgttctgg tgcagcggcg cgtagttctc
    20281 ctcgtggagc accgcgccgc cgccggtggc gacgatcgcg ccgaaggcgc tgccgagctc
    20341 gcgcacggcc tcgctctcgt gcgtgcggaa cacggattcg ccctgcattg tgatgatctg
    20401 ctcgggcgta tagccggtgt gctcctcgat ataggcgtcc gtatcgagca cctcgcggcc
    20461 aagcgccttg ccgagcgcct ccgccacggc ggacttgccg cagccgggca tgccgatgag
    20521 gacgatgttc atcgtcttgc tcttcacgag gtcgatggcg tgctcgagcg cgccggacgc
    20581 cgcctttttg ccggtgaaga tctcgctcgc gcggatggcc tgcgccgcga gcatcaccat
    20641 gccgttcgcg caggggatgc cgagtttttc cgcctgcagc aaaagctccg tgcgcacggg
    20701 attatagatg agatcgacga cggcgcggca gtgcgggaag cgcagcagat cgatgaccgc
    20761 ctcgccgttg tgcgggtaca tgccgaccgg cgtggtgttc acgatgagct cggcgtccgt
    20821 gtggcgggag aggttggagt aattgtcctt cccatcgcgg gagacgacga cgatctcgcg
    20881 cgcctgctgc ttttcaagcg cgtacaccac cgcggcggaa gcgccgccgc tgccgagcac
    20941 gagcaccttt ttcgcgcgcg gcgagaatac cgcgcggcgc agcagctctt caaagccgta
    21001 gacgtccgta ttgtcgccgt agagcgttcc gtcggcgcgg cggacgatcg tattgacgct
    21061 gccgatggcg cgcgccgttt cggagagctc gtcgcagagt gccgcggcgc actttttgta
    21121 ggggatcgtg acgttcagcg cgtcaaaatc gcccttccga aggaaggtct ccacattctc
    21181 cggttcggtc tcatagagct tgtactcata gtcgccgagc atggaatgga tggccggaga
    21241 atagctgtgg ctgagctttt gtccgagaag tccgcaatgc atcatgtcaa attacctcac
    21301 tatagcttcc aagataggtg tattcctcgc tgatgtcccc cagctcactc acaagctgaa
    21361 ggaaggcggg atcgtacacc gggttttcca gatcgaaata gaacatgaac tcaaagctgc
    21421 gctccgggag cgggcggctt tcgagcttgt tcaggttgat gccgagcgca taaaagcgcg
    21481 agagcacttt ataaagcgag ccgggttcgt ggcgcagcgt catcataagg ctcgtccgat
    21541 ccgcgccggg atagatctcc agatcgcgcg cgatgcagat gaagcgcgtg tagttgttcg
    21601 ccgtatcctg cacagagggg gcgagacatt ccagaccata cagccgctcg cacgcgcgcg
    21661 tggagagcgc cgcagcgtcg tcccgcccgg actccgcaac gagctttgcc gcgagcgccg
    21721 tattctcgca cgggatgacc ttcacgcccg gaagcgacga gaggaactgc gcgcactggc
    21781 tgagcgcctg ctcgtgcgaa tagatctcgc ggatgccgga gagctttgcg ccgggcctcg
    21841 cgagcaggtt gtggtcgatc ttcacgcgca ccgagcggac gatgtggaaa tggtggcgca
    21901 tcataagatc gtacacggcg ttcaccgaac ccgcggtgct gttttcgatg ggcagcacac
    21961 cgtagcggca gaggcccttt tcgatggcgt tgaacaccgc gtcgaagctt gaaaagtaaa
    22021 gctcgttcgg caggcggaac agccgctcgc acgcgatctg ggagttggag ccttcgacgc
    22081 cctgacaggc gacggcggcc ttctccggga agagcggcgg cgtctgctcg atcgctgcct
    22141 gaatgctctc ggcgagcggg ctctgcttat gcagcaggcg gttctggcgg ctgcggctga
    22201 gctcaaagag cagcgcgtag agcgacacgg cgtagctttg cagctcctcc ggcgcttttt
    22261 cgctcacggc gtagagcttt tcccgctccc gccgctcatc gagcaccgga aggccgtgtt
    22321 cggctttata ccgggcaatg tccccggcga tgtccatgcg ctcggcaaag catcgcagca
    22381 gctcgtcatc caccgcgtcg atcctgcctc gcagttcgtc catgttcatg gctttctccc
    22441 tctttccgaa caaagaatat catatagcac gacnnnnnnn nnnggcttga acgccgcgcg
    22501 gaacacgagc ggcgcgcccg tcgtgatgcc gccgaggata ccgccgcagc ggttcgtctc
    22561 cgtcatagcc ctcccgccgc gcagcacaaa ggcgtcgttg ttctcgctgc cgcgcagcgc
    22621 ggcggcggcg aaccccgcgc cgaactccac gcccttcaca gcgggaatac cgaagagcgc
    22681 agcggagaga cggctctcca agccgtcaaa aagcgcgtcg ccgagccctg cgggcaaacc
    22741 gagcaccgcg cactccacca cgccgccgac gctgtcgccg tcggcgcgtg cctttgcaat
    22801 ggcagcaagc atctcctcgc cgcgcgcttc gctgagcgtc gggaacggtt ttcccagtgt
    22861 ggatgccggg aagatgccct cgtccgcgat accggcaatg gaggcgatgt gcgaaacgat
    22921 gcggatgttt tcctcttcca gaagctgcat ggcgatgccg cccgcaacgc agagcggcgc
    22981 ggtgagccgt ccggaaaagc gtccgccgcc ggagagatcg acggcatcgc cgtctttcat
    23041 gcgggcggca aagtccgcgt ggccggggcg cggcgtattg cgcagagcgt catagtcgcc
    23101 gggatgggcg tcgccgttgc ggatgaccgc cgtgaccgcg ccgccgcagg tgacgccgtt
    23161 tttcacgccg ctgagaaaga gcggcgcatc gctctcgcgc cgcgcggtgg caaacgcccc
    23221 gcgtcccggc gcgcgccgtg tgaggaaggc attgagcgca tccatatcga tggcaaagcc
    23281 cacgggcagc ccttccataa cgacgccgat ggcctcgccg tgcgaagcgc cgaagatcgt
    23341 gatgcgaaag ttttttccca gactgctcac ggcgtttcct cccttttcaa agcgttaaag
    23401 tcctcgccaa agcgcggata ggatttgttt gtgcactccc agccctcgat cgtgaccggt
    23461 tccgtgcagg cgcacgccgc caccgccgcc gccatggcga tgcgatggtc gtgctgcgcg
    23521 ctcaccgcgc cgccgcgaag tccgccgccg tgaacgatga gtctctccgg cagctcctcg
    23581 acgtgcgcgc cgagagaagc gagcattgcc gcggtcgttg cgagcctatc agattccttg
    23641 agccggagcc gcgcggcatt cacaacgcgc gtctcccctt ccgccgccgc agcgagcgcg
    23701 cagagcgcgg ggatgagatc ggggacgggc gcagcgtcga tgaccgcgcc gtgaagcgtg
    23761 ccgcgccgca ccgtgatgcc cgcggccgct tcctccacgc atgctcccat cgcgcggaga
    23821 atatctagca cggcgcggtc gccctgcgca gaatcggcgc gcaggccgga aacggtgatg
    23881 ccgcagcgcg agagagcgcc catgcagagg aagaacgccg ccgaggaata gtccccctcg
    23941 accttgaccg cggcgggcag ggcgtacgtc tgcccgccgg ggatatcata cccggaatcc
    24001 gttttaaata gccggacgcc gctttgggtg agggcgttct ccgtcatggc gatatagccc
    24061 tcgctctccc gcgcgccggt gatcgtgagc gcgctgtccc cggcaaggcg cggcagcgcg
    24121 aaaagcagcg cggaaatgta ctgcgaggaa atgtttccgg ggagcgtata ttctcccggc
    24181 gcgagttttc cggagcagaa gagcttcccg ccctcggatc ggaacatcat gccgccgcgg
    24241 acaagctcgc tcaaaagcgg ttcgagcggg cgctcaggca aacggccttc ccgcaggaac
    24301 accgcgtccg cgccgagcgc gccaacgacc gggagcaaaa accggagcgt cgatccgctc
    24361 tcgccgcagg gcaggacaca gagaccgtcc ggcactgctt tgaccggctc gacacgcagc
    24421 gcgccgtcct gctccgcgat gttcgcgccg agcgcgcgaa ggcacgcggc cgtggcaaga
    24481 atgtcggcgt tcagcccgtc gcagcggacc gttaccggcg ctttgccgag cgcggcgcag
    24541 atgagcaggc ggtgcgcctg cgatttggaa gccgggatgc gcaccgcgcc ggtgcgtgct
    24601 cccggagaaa tgacaaagtt cattttgcgc ccccctgtcg gagccatccg gcataatcgg
    24661 acgcggcgat ctttcgcacg gtgcagcgcc ccagcccctc aacggtgatg acgggaagcg
    24721 ttgagccggc gcgtttttta tccgaaagcg cggcgttggc gagcgcttcc gccgtatacg
    24781 gcagctccgt cggaaggccg cattttttga taagcgcttc gatgcgcgcg gcgtcctctt
    24841 cggtgcagag accgcgcgcg gcgcacgcgc gcgccatgga gcacagcccc atcgccacgg
    24901 cgctgccgtg ggagagtgaa taatcgctca aaagctccgc cgcgtgcgcg gcggtatggc
    24961 cgagattcaa aagatgccgc gcgccggtat caaattcatc cgcagcgaca atatcccgct
    25021 ttatctccac gcagcgcgcg agaatatcct ccggtatttc gtccggcagg ctgttttcca
    25081 gccgggagaa aagcgccgcg ccgtccagta cgccgtattt gatgatctcg gcgcagccgt
    25141 cgcgccggac ggcgtccgga agcgtttgga gcgtgtccgt atcgcatata acgagcgacg
    25201 gctgccagaa cgccccgata aggtttttcc ccatcgggag atcgaccgcc gttttcccgc
    25261 cgacggaaga atccaccgcg gcgagaagcg tcgtcgggat ctgcacgagc cggacgccgc
    25321 gctggtagca cgccgcggca aacccgacca tgtcgccgac aacgccgccg ccgagcgcaa
    25381 cgacaacgtc gctccggctc acgcggtttt cggtaaagaa acggaggata cgctcccagt
    25441 tgcgaaacgt tttggagtgt tctccggcaa gaaagtagtc ctgcaatacc gtaaagcccg
    25501 cggccttgag cgactcggtc acgcgctttg catagagcgg aaacacggtc gtatcggtat
    25561 agacgaccgc cttttccgcg cccgggacgg ccgcggcaat gcgctgcccg cattcatcga
    25621 gaagccccga gccgataaca acatcgtatt cgcgcgacgc cgttacatgg atcgttttca
    25681 tcgtccgtct acctcctcct tgcgccggcg gccctcgtcg agaagcgccg tgagcgccgc
    25741 gagatcgtta ttctccaccg ccgcgcggta ctgcgcgagg gactccgcga gactgtccag
    25801 ctcaaagagc acattgtccc ggttatccag aaagagctcc gcccacatgg aagggtcgag
    25861 ccacgcaacg cgcgtcaaat ccttatagct ccccgcggag aaacccctgt ggctgcgcgc
    25921 ggtggggctt ttgatgtagg cattcgagac gatatgcgcg agctgcgacg taaaggcgat
    25981 catgcggtcg tgctccgcgg cgctcgtcac ggaaaatttc ccgaactccg ccggggccaa
    26041 aagctccttg gcccgcgcga gaagggcgat atcgtctccc tccggcggta cgagcaccat
    26101 gggcgctgca tggaaaagat tggcgcgggc attcttatat cccgcgcggt ggctgcccgc
    26161 catcggatga ccgccgagaa acatgaagcc gtgctcccgc gcgagagcga acgcggtctc
    26221 gcacacctcc cgcttaacgc cgcagcagtc gatgacgacg gggcgcgcgc cgatatgcgg
    26281 cgccgcttcg cgcagatacg atatcgccgc gccggggcgc accgcgatga gaacgatatc
    26341 gcattcggaa acattttctt ttgtcagcac ggcgtccacc gtgccgtcga gctgcgcgaa
    26401 tgagagcacc gcgccgtccg tatcaaaggc aagaacgcgc gcaccgtgcg cgcggtacgc
    26461 cttggcgagc gagccgccga tcagcccgag gccgatgatc cccgctgtca tcgcattgcc
    26521 acctcgcgga tagtccgcac gtccctgcag agcgcgtcgt actcctcggg cttgatggac
    26581 tgcgcgccgt cgcacagcgc gtgcatcggg tcattgtgga cttcgatgat gagaccgtcc
    26641 gtgccgcagg ccgccgcagc gagcgccatc gggcgcacca tgcgggcaag accggtagaa
    26701 tggctcggat cgacgatgac cggcagatgc gtcagctcat ggagcatcgg cacggcggca
    26761 agatcgagcg tgtttctcgt ataatcgtca aacgtgcgga tgccgcgctc gcagaggatg
    26821 atctgctcat taccgcccgc catgatgtac tcggcgctca tcaaaagctc cttgagcgtg
    26881 gcggcaagac cgcgcttgag cagaatgggc ttgcggagct ttccgagttc tttgagcagg
    26941 tcaaagttct gcatattgcg cgcgccgacc tggatgacat ccacatccgc gaaaagatcg
    27001 agcgcggagg tgcccatgat ctccgtgacg atcggaaggc ccgtctcctg ccgggcgatc
    27061 ttcaaaagct cgagtcccgt ggcggcaagg ccctgaaaat cgtagggcga ggtgcgcggc
    27121 ttgaaagcgc cgccgcggag gatctgcgcg ccggaggcct tgaccgcctt tgcgatgccg
    27181 acgatctgct cctcgctctc gacgctgcac gggcccgcga tcatcacgaa gttcccgccg
    27241 ccgatgcgca cgtccccgac ctgcacgacg gtgtcgtccg ggtggaactt gcggttgcag
    27301 cacttgaacg gctccgtgac gcgcttgacg gaatctacga tgccaagact ggcgataagg
    27361 tccatatcga cccggctcgt gtcacccacg agaccgagaa cggtctgata ctcgccgacg
    27421 gaggtgtgga tctgcagtcc ctgctccttc agccagtcaa taagctggtt tttcttnnnn
    27481 nnnnnntttc agaaaataca aacaaaaaac gcggcacagt gtaatctgtg ccgcctaaaa
    27541 agccttcgct tccaaggtca ttttccgcat cacaaattac catactatga aatagtacgg
    27601 gtaaaaaagt atcgaaacat acgaatggac acattcatga tgccgatctc gctccaatct
    27661 gcggaatttg gtttcagtat aaattcttct cttggaaatg tcaacaaata attcggtgaa
    27721 aatttgtata aaaatttatt tttgatccgc ttcaagcctg tctatcacgt cgcggatgca
    27781 gcgtccgggt gcgccgagct tcgtcacgcc gggaagcgcc aaaacctccg gcagcgccgt
    27841 ttccggtgcg aaggcgcggc ctgccgcgcg cagcatcgga atatcgttcc agccgtcgcc
    27901 gatgcagacg atgttttccg gaagaatatc cagaaactcc gcaagccgcc gcacgccgct
    27961 gcccttgtcg ctcccggcgg cgacaacgtc caccagagcg ccggagggca cggcctcgta
    28021 ccgtccggga aactgcgctg cgatccacgc gcagagcgcg tgcgccgttt cgctctcggc
    28081 gcggatctcg tccggcgcta caagcaccac cttcgtccac ggatacagaa tatgtcccgg
    28141 atcggcctcc gaaaccggca cgcccatacg ggaaaggtgc ttctcgagcg tcgcattgct
    28201 gccgcagacg cgcacatccg cgccgcggtg gacctccatg cccgtgccgg gaaaggcgtc
    28261 gcgcgcggca agaaggtcgc tccggcagga aaacggcaga aagctctcgt accagctctc
    28321 gccggattcg ccgtcgaaga tcacggcgcc gttgctgagt acgagcggcg cgttgacggt
    28381 gaatttgtcc cggatggagt aatacgagcg cacgtcccgc cccgtggcga tcgtgaaaag
    28441 gccgccctcg tccatgaaat gctgcgccgc gcgccggttt tcctccggca cgggagcgcc
    28501 gtgctccggg cagaatgtgt cgtcaaaatc cgcaacgagc agatatcctt caaattttcc
    28561 gtttcccatt ggtatttctc ccaaaactat gtataatgaa ctttcagtgg cttctccccg
    28621 tctttccggc aggagatctt ttccggaggt acgctatgac aaaaaaagaa caacgcatca
    28681 accggctctt tctgtatgcc gggatactga cgcttatata ttatcttggc cagggcatca
    28741 ccgtgcggtt cgggcagtcg ctgctctatc tctggctgat gctgtcggcc gtgtgctttg
    28801 gccgcttctt tctctggaaa tgggcgtgga agcacggaaa gagcgcgccg atcccccggc
    28861 cgtggctccg ggcagtgaat atcgtcgtga tcgccgccgt cgccgtattt ctcgccgtgg
    28921 agtgtctggt attttccggc gcgttccaga agcccccgga ggggctggac gccatcatcg
    28981 ttctcggcgc gagagtcgat gaaaacggcc cgagcgggtc gctgcggcag cgcatcagcg
    29041 ccgcgcgcgt ctatctggag gaaaacccgg aagcccagcg gcgcgctgac gcagcggata
    29101 tgggcggcgg cggattacct cgccgcaaat ccggacacgg tatgcatcgc ctccggcggg
    29161 cagggcaagg acgagccgat gagcgaggcg gaatgcatcc gcgatcatct cgtcgcggac
    29221 ggcatcaacg cagaccgcat ccttttggag gaccgctcca cgagcacgga ggagaacatg
    29281 cgctacagtc tccccatgct gcgctcgccg gagacagacg tggagagcgt cggcatcgtc
    29341 acgaacgact ttcacgtttt ccgcgcgctg tgtctggcgc ggaaaaacgg cggattcacg
    29401 ttttacggcg tcccggcccg ctccaccgtg ttcggcttca tccactacgc gatgcgcgaa
    29461 tttttcacgc tgagcgtgtc gcttgtccgc ggatacatct gattaaacca caaaaacggc
    29521 tctccgttgt agggacggag agccgttttt tatcggggca gcccctccgt ccgcgttcag
    29581 gccgcgtttt cccaggcaag gcagagcgct tccatatcct ccaccgcctg cgaaacgcac
    29641 gcgggcaggc cgtcggtgaa gcattcgagc ccgaccttgt caagcagctc gcagtacgtg
    29701 taatcaaagg cgccgagcga gacgagatca ttatagaagc ccgctgccgc ggcccagtcg
    29761 ttctccgcca tttcgtagat ctgcagcgag gcgagcatgc tcacggcgta gctgatatag
    29821 tagaagggcg actcgaagtt gtggttgacc tgcatccagt agtgcgaaaa ctccttgccg
    29881 aaggatcgca ggccgtaaga gcgcgcgatg gtcacgaacg tctggctgat ctgctccggc
    29941 gtcagagtcg ggtcggcgta gaccacgcgc tggaactcgt cgaacagaca gccggaaaag
    30001 acggtgtaga gcgcgctgtc aagaaaccgg atgcgcgcga gatcggcatc cccgccgtag
    30061 atatcctcat accagccggt ggaaagggcc tccatgccgg tagagtggat ttcaaaaatg
    30121 tccagcgagc cgacgctcag caaaaggtcc ggcacggggt tcacataggc gtcgtaataa
    30181 tggccgaact cgtggaacat actctggatg tcgttctggt cgccatagag cgcgagatag
    30241 atgaaggggg cattatactg gtcgagcgtc gtggtgaagc cgagatcgct gacgcgctca
    30301 gcgctgtcca tgaagtacag cccgtgcttt tccatatacg ccgccgcttt ggccgcgtcg
    30361 tccgagacgc ggggcatata ctgcgtgacg agcccgacga gctcccgctc ggtaaggtcg
    30421 gtgtccgcgg agaaatcggc atacgtcgcc tcattataat agagcgagcc gaaatactgc
    30481 cgcgcgtacg gcttcaccgc ttcacacagc gccgcggcat cgtcgggcgt aaaatcgcgc
    30541 ccgtagctcg cctcgtaggc atagtcggca tagtctttat agccgacgat ctgcgcctcc
    30601 tcgttgtgga gatccaccag ctcgcggtag agtgcgccga tcttctcggt ctcctcgtca
    30661 tagctgagat attccatgat gagcgcgttg tactgcagcg tcagctcgga gacgcgctcg
    30721 gtgatctcgc gctgccggtc ggtctgctca tcatagccga ccagagcggt tgccttgtcc
    30781 tcgccgatat acgagcgcag cgcggagccc gcggggccgt cgagcgcgtc ggagagcgcg
    30841 gtgcagagcg cgtcgttcac ctcgttaaga acgctgtcga gctgccggca ggcgtcgctg
    30901 agcgtctcat cgccgggatg ggcgtagaaa tcgatgtagg cgagattgtc aaacgtgtac
    30961 accttggcat actccgccat cagccagtcg taaaggtctg ccgcctcttt gccgtccgcc
    31021 gtttcgccaa gccgcgcagc cttgtcgagc agctccgcgg catcgtagat gtaccacgtc
    31081 atatcggcat agtccacgtc ggcgtggatg ccgtcggccg aggcgaaccg gccggtctcg
    31141 tcctccggca ccgtcacgcc gaggccggtg gcgagcgcat cgctcacgct gccgtcactg
    31201 cgtactgcgc cgaccgtccc ggcgtcatcc gcacagccgc agaggatcag ggaaagcatg
    31261 gccgccgcaa ggagcagcgc tattcttttt ttcattttca tttgcatcac ccgtttccat
    31321 actacacgat tcctcgccga aagtaaatag cgcgccattt tccccttgct accgcgggaa
    31381 aaataggata taatccgccg tatgaaaaag attcgtaaac tgattgaaaa ttttcagcac
    31441 atttcggact acatgggccg gcggcagatc gccaccattg ccgcctcgac ggcgttctgg
    31501 ttttttctct ccatcgtgcc catcgtcatt ttgtgcgtgt ctgttcttcc ttatacgtcc
    31561 atcaccgagg aacagctcct cacgacggtc gcgcccgtgc tgccggactc gatgaaccag
    31621 ctcatccgcg tcatcattgc ggacgtatac agcagcggcg tcggcgtttt gagcatttcc
    31681 atcgcggcga cggtgtggtc gtcggcgatg ggctttgcct cgctcattcg agggctggag
    31741 gatatttatg agcagccgcg ccgcgccggg tttctgatgc gccgcgcccg cggcatcatc
    31801 tatacgctcg gcatgctgct tatgatgatc ctttcgttcg tgctcggcgg cttcggccag
    31861 cagatcaagg cgctggtgga aaaatacctt cccggctcgc agtcgttctt ctcatttctg
    31921 ctgcacttcc ggttcgtggt ggtcatcgcg ctgctgacga tcttctttgc cgccatcttc
    31981 cgctggagca ccggactgcg cccgcgcttc ggcgaggtgc tgcccggcgc gctgaccgcc
    32041 gcggtcgggt ggtcgatctt ctcgtgggcg ttctcggcat gggtctccgc cacggggagc
    32101 tacggtacct acggcagtct cgtaacggtc gtcgtcgtga tgctctggct ctattactgc
    32161 cagtacattc tgcttctgag cgcgtgcctg aaccgcgctc tgccaaagtt ccgccgccgc
    32221 ggagaaacgg aaccggcatc cgaaccggaa ccaagcgaag agtaaaaaaa atccgtatgc
    32281 ttttcgatct cgatgaagca tacggatttt tataatacta ttgacctttt acctccgtgc
    32341 gccggacgcg ccgagcctgc cggagagcca cgaaaggagc gccgcggcga tcgctgcaat
    32401 gccggcggtc tgccacggga gcgagagcgc cgcgccgccg agcgagccga gcagcgcgca
    32461 aagcgcccac gcaacgcccg ctccgagcac gccgccgaaa aaaccgagca gcaggctttc
    32521 cgcgccgacg atgccctgcg gcgacgaaag ccccagtatg cggagctgcc aaagctcgcg
    32581 gcgccgaggc ggccagcgcc gagacaagca cgatcaacgc gccggcgagt acgcccgcga
    32641 gaaccatacc gagccggtcg gtcttttccg cgctttccat gagcgccatc cccggctgca
    32701 caaggccgga ggcggcgtcg gcataggtca cggtctcggc atagccgcgc agcaccgcgc
    32761 caagcgtttc gcgcgcggag aagctgtcgg cataaagccg cagctgcgtc acggtctgcg
    32821 ccgcctccgc gccgaacgcg cggagatttc cctccagcga gccggaggaa acgccggagg
    32881 cgatataccg ggcgaaagcg ttttccagcg cctcgccgga gagcgaggca atggtctgca
    32941 aaagggcgtc ctgcgcccgc tcggtctccg tcgccgttcc ggtgagatcg agcaccatgg
    33001 ccgcctgctg cgccggggtc tgtccgacgg caaagccgcg cagcgcggcg cgctgtccgg
    33061 tctcgtcctg cgcatacaca ccctcggtgt cgaacggcag gccggtcaga acatccgtat
    33121 cgggcgaggc cgtctgcgct tttacgatgt cactgccgag caccgtctcc acggtccacg
    33181 tcatgagctc cggcaggtaa gccgcgccgc cggtcagact ctcgccggga gtctcccccg
    33241 ccgggcacag aatgccgacg atgttcagcc gctgcgaaga agcgatcttc gcggtgagat
    33301 acgccgtatc tccgcccata tagccccagg tgccgtccac attttggacg tactcgtccg
    33361 tcggcagcag cacacggaac gaaagacgca gaagctcggt atagctgatg ttggcgtttg
    33421 cttccgggtc aagaccaagc gagcggaggc agctctcgtc caccttgccg cgcgcgtcga
    33481 gcagcaccgc cgcctcatcg taccgctccg gccagcggcc ggcagcgagc gtatagcgca
    33541 tctgccgcag gtcctcccct tccgggagct gcgtccagag cgtgttgttc tcctcgctgt
    33601 tgagcgcttc cgtcccggcg gaggatgtat cgccgctgta aatgcgcggt gtgatggcgt
    33661 aacggcgctg cacggagatc gttttttcgt ccgtgtgacc gtccagccag tcgccgagcg
    33721 ctccgagatc gccggaggga acgcttcctg tgtcgagcac gaccggatag gcggagagca
    33781 ccgccgtctc cgcgtccttc gcgctctgct ctccgccgga aaaggcagcg aacagcaaaa
    33841 ggcagagcag caccgcaacc atcggcgcga gcaggcgcgg cagcgtatgg ctctttttct
    33901 ttgagaaatt ggagaacacc atgcggaggc gttccccgcc ggaggccccg gcgacgctct
    33961 ggtgcgccgt ggccgcgctc tccgcgtcct catccgtgtc ggaggcgatc tgcccgtcct
    34021 ccagcgtcag gacgcgcgcg tcctcgccga acggctcggc attccgcgcc gcgacgatga
    34081 cgagctgatc tgccgccgct tcgccgagaa gcgagagcac ggtctccgcg ctctccgcgc
    34141 tgagaccgtc tgccggctcg tccagaagca gcacgcggcc tttgcgggcg agcgcgcaag
    34201 cgagcgcccc gagacgccgc tgctctccgg agaggcggtc cgggtatacg cccgccgcgc
    34261 cctcgagccc gagcagagcg agcgcttcgc gcgcattctc ccgcgcgtcc cggccggaga
    34321 agccagcgag caccgcggcg cgctccgcgt tttccgaaag cgtgagatcg gcaaagagaa
    34381 gcgcttcgtc cgcggcggcg cattcccgca gccaggcgct gcgccgcgct tcgctccagc
    34441 gcgccgtatt ctcgccgccg atgaggattt cgccgcggga gggcgtctca cgcagggcga
    34501 gcagatgcag cagcgccgtt ttcccgctgc cggacgcgcc ggtgagcacc acgaggccgg
    34561 tctccggcag cgtgacattc aggcagcgca gcgcatgcac gctctttttt ccggcggagg
    34621 attccagcgc ggcgtttttc agttggatca tagaaaggcc catcctttcc cttttataca
    34681 gagcaattat accttttgca taaagcgcgg tcaagtctcc ggcgcaattc gtcaaagttg
    34741 cgcttgcaat tccctttttc gtgcggtatg atgtgcgtac agaacagggg tgctgggttt
    34801 tcccggctga gacggtgcga atcgaagcgc cggtaccctt ttacctgatc cgggtaatgc
    34861 cggcggaggg agtgcctata cggtatttgt tcttcagcca cgtccgggat cgggtggctg
    34921 atttttttat tggagggctt ttttatgaaa agcaaacaga ccacccgcac actttgcgaa
    34981 ggagcgatca tggtcgccat ggcggtcgtg ctcaacttct tcaagttcga tctgttcgcg
    35041 cagggcggat cgatgaacct gctcttcatt cctctgatgg tgttcgcgct gcggcgcggc
    35101 ccggtctggg gcatgggcgc gggtctcgtt ttcggcgttc tcaaggccat catcggcggc
    35161 ggcatcgcct acggctggca gtcgctcctg ctcgactacg ctctggccta cgcgctcacc
    35221 ggtcttgccg gtttcacgcc gaaaaagccg gtgctcggca ccgtcatcgg cgcgctcggc
    35281 gcgcttctct ccttcgtcct cagcggcgtg ttcgtctggg gcgagtacat gccggacgtg
    35341 ttcctcggca tggcgatgaa gaacgtgtgg atctactcgt tcatctacaa cttcatctac
    35401 accgctttca actggctcat cgcggcaatc gtcatctact tcctttccaa aaagacgaag
    35461 ctcctgcagc ccgaaaagta aaaacgtttt tactcatcct ctcttttttc cgcgcgccgc
    35521 ctctccggga gaaccggagg ggcggttttt tcgtctataa agatagaagt cgtttattat
    35581 atttggatta aggaacttgt caaaaggcga cattctggtg tatactaatt tggtgtcttt
    35641 atattactgt tgagagcgag gatgaaacca tgcgtgaacg cagcgcagcc ggtgggatct
    35701 tccgcgtcgt tggacgcatt ctgctgatcc tgctggtcac catactgctg tttgccgttt
    35761 tcctgcttgg cgtgatgggc gtgctgatcc acggcccttc ggccgaagca gagcggcttt
    35821 tcgtgctgtc cgttaatgag acgagcgcgc tgaaattcct gccgcacatc tatctttccg
    35881 atgcgcaggt ggacgcgata ctgcaccccg tcagcacctc cacggacgat gaaccggcgc
    35941 cggacgacaa ttttgtcgag ctgccctacg aggacggccg caccgtggaa gacgaaaaca
    36001 ccaacgcggg acaggtcgtc gtggccgacc cgtcagagaa caccggtgag agcggcgagt
    36061 ggcttgaggt cgtagacatc aagggctcga cctttaaggg caagctcatg atcatccgcg
    36121 atccgtcaaa ggtcgttgtc ggcacgctgg acgcttacgg cgcgaactat cacggactgt
    36181 atctttacga gtttattgat aagtataacg ccatcggcgg cacgaacgcc ggcggcttct
    36241 acgatccggg cggaggcggc aacggcggca tccccgacgg tctcgtcatg cgcgacggcg
    36301 ccatcgccta cggcgatccc ggcacatggt atctcaacgt tatcggcttt gacgcggatc
    36361 atatcctcca cgtcggagac atgtccggcc agcaggcact cgatctcggg ctgatgagcg
    36421 cggtcagctt ctcccccggc ccggtgctcg tgaaggacgg cgagctgcag acgaatctcg
    36481 gcggcggcat gaacccgcgc acctgcatcg gccagtgtgc ggacggcacc atccttctga
    36541 tggtcatcga gggccgcaag cccgacagca tgggcgcgac gtacgacgat atcgccaaga
    36601 tcatgtatga ccgcggcgcg gtcaacgccg cgaacctcga cggcggctcg tcctcgctca
    36661 tgtactacaa cggtgagcag atcacccgcg gctcgaacat catcggcatg cgccagatgt
    36721 ccaccgccat tctcattttg aattgagggc agctatggga aagcatgaaa aacctgtatc
    36781 cggcacgagc cggaagagcg aagcgcgcct gccgcgcgcc gagcgcactc cctccgtccg
    36841 tgaagaggag ctgctctttg atattccgga gaccgccgag cgcgtgactg cgcacgcggc
    36901 ggagacgctg ccggaagagc ccgccgctcc ggcccccgcg gagagctttg aaactccctc
    36961 ccccattccg gaggagagct ttgaggcgcc cgcctccatc cggagagcag cgcgccccgc
    37021 gcaggaagcg gccccggtgc gccgcgaacg ccctgtgagc gaggaaaagc ccgcccgtcg
    37081 ggaacgtccc gcgcaggata gtgagtctgt ccgccgcgag cgtcccgcgc aggagaacaa
    37141 gcctgtccgc cgcgagcgtc ccgtgcagga ggacaaaccc gcgcgtcggg aacgccccgc
    37201 gccggaagag aagcctgctc gtcgggagcg tcccgcacag gaaacgaaaa gcgcgcgccg
    37261 cgaacgtccc gcccgtgcgg aaaatcccgc cgcggacgag gaaatcgttc cgaaaaaccg
    37321 aaagaaaaaa ggcggcttcc gcaagggtct gctgacgtat gttcttgtcg ttgcggtgat
    37381 cttccttgcc gcgctcattg tcctttgggt cgcgcttgcg cgcttccaga aaaacaagga
    37441 cgccgaaaaa gccg
//
LOCUS       DAWAAE010000002        36176 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_489_length_36176_cov_3.364414, whole genome shotgun sequence.
ACCESSION   DAWAAE010000002 DAWAAE010000000
VERSION     DAWAAE010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 36176)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 36176)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36176
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_36176_cov_3.364414"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2915..2924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4945..4954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5289..5298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13474..13483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6812 a        11418 c        10822 g         7084 t
ORIGIN      
        1 cgctcgccgc agccgatgta gacgacgata tccacatccg accactttgc aagctggtgc
       61 tgcacaacgg tcttgccgct gccgaacggg ccggggacgg ctgccgtgcc gcccttggcg
      121 atggggaaca tggtgtcgat gatgcgctgt ccggagttca gcggacggct gggggtgaat
      181 ttgccggcgt tcgggcgctg gatacgcacc ggccagcgct ggatcatatc cagctcaatc
      241 tcgctgccgt cgccgagcgt caggcaggcg atcttttcct cgacggtgaa ttcgccgccg
      301 cggatccact tcacggtgcc cttcttgatc ctcgggggga ccatgatctt gtggaggatg
      361 gcggtcgtct cctgcacggt gccgagcacg tcgccggcga cgagttcgtc gccctccttc
      421 gccgcgggga cgaagttcca gatcttcttg cggttgagcg ccggaacgct cacgccgcgg
      481 gcgatcgtct cgccggtgag atcgcggatc tccggcaggg gacgctggat gccgtcatag
      541 atgttttcga gcatgccggg cccgagctcc acgctcagcg gcataccggt cgtcacgacc
      601 tcggcgccgg ggccaaggcc ggaggtctct tcgtagacct gaatggaggc gctgcctccg
      661 gtcatattca gtatctcgcc gatcaggtgc tgttcgccga cacggacaac gtccgatacg
      721 ttggcgtcct ccagaccgtc ggccaccacc agcggtccgg atactttaat cacttttccg
      781 ctcaatgttc taccttcttt ccgttaaggt tccggcgcgg cgctgccgcc gcccggcgcg
      841 ccgcatgcgg cgcaaaaggg tcgtctttat aacttacagg atattgctgc cgacggcgcg
      901 ttccaccgcc gagcgcagcg cgctctggcc gagaccgagc gtcccgtccc gtccggggat
      961 gaggatgatt gccggggtgg ggctgtcctt gaagcggtcg atctcatgcg agagctccgc
     1021 ggcaaggttc tcctccagat agacgatggc gtattcgctc tcgccggagg tgatccggcg
     1081 gagagtgtgc cgcgcctcgt ccgcgctgtc cacggggaac acgtccagcc cgagcgcttt
     1141 aaagcccata acggtctctc tgccgcccat gacgccgatc ttatacatag ctctcacgca
     1201 gcctttcttt cagtcgctcc gggtcgatgc cggccagaag cccggtgagc accatgcgaa
     1261 ccgccatggt gttgccctcc tccgccgtca gatagccgac gacctgttcg gggccgaagc
     1321 tcttgagatg cgcgccggtc atgtagcggt tgacggcgtt gtcgcactca cgctcaaact
     1381 ccgtcatcgt gccgccctcg atcgcggccg cgccgagcgc ggcggcatcg ctgaacggcg
     1441 tcgaagcgaa cgccgcggcg atcccctcgc cggagaacac tgcctgcacg agccgctgca
     1501 ccggaacatt gccgcccgcg acgagcgcgc cgcggagaaa ctccgcgtcc ttgcccatgc
     1561 gcacgcagcg caccgcggcg cggaggttcg cgctgtcgat gagcgttgtg acatagcctt
     1621 tcacgaaggg gctcgacacc gcgtcggcca gcgccttgag atcctcatag tacgccttgt
     1681 cgagcgcgaa gtccgccagc tgcggattgc cggtgcgggc aagaatgccc ttggcttccg
     1741 ccatggcggg gccgagcttt ccggggatga actggaagct gtcctcgcgg aacgccgctt
     1801 cgagcttttc cggctcgacc tgtccggcgt cggagaggag gtacgccgca tcaacgcccg
     1861 cgccctcgcc cttgacgatg gttttggcgt tgtggtagtc gtacttcagc cggaagatgc
     1921 ccaccagctc cttttccgga acgaaacggg tgatctccgc atagatctcc gcctggcgct
     1981 tggcaagcgc ctcgctcacc gccgcgctgc ccttgccgga aagatcgggc cagccgatct
     2041 cggcgaggag ccttgcggct tcctcaaagc tgccggagga gagcatctgc tcgagccggt
     2101 cacggcggag catgcggttc tcccgtgcct tgagcatcga ggagatgaac agataatctt
     2161 tgtccgaaac ttttttgttt gccattttct tcccgccttc caaccggatc agtcaaagag
     2221 gatggcggcg atctccgcat ccagactgtt ccggctcagc gcaagaagcg tgtcaacggt
     2281 gcagttgact tccacgctgc cctcgcggag cttgagaccg ccggagaagt cgccgctctc
     2341 gtccgagagc gtgagcccgg cggggagtcc ccgctcggtc aggatcgcgt tcgcgccgcg
     2401 gacgatcgcc tcgcccatgg agcggtcctg gctgttgatg acgatgcgtt catgcccggt
     2461 gacggcggca tggcccgcct gggacgcgag gaacgccgta tatttttcct ccggcagggc
     2521 gaggagtttt tcctttgctc tgccgtaggc gcgctcgaga atgctctgct tgagcgcgag
     2581 cgtatccttg cggctctgga gctttgcggc gcgctgttcg cgctcggcgc cgaggcggac
     2641 ctcggtattg ccctcgttca tgcgcgcttc atacgcctcg tccgcttttt tctggtaggc
     2701 ggcgcggatc tcccccgccg tcttttcggc ctcggcgatc acggcggcgg cggcttcgtt
     2761 cgcctctgcc gtgatacggg ccgtgatttt ttcaattccg ttcatggcga taccggtcct
     2821 ttccggatca ggcgccgagg tgcaggttca gcagcatcag gaaggaggcg agcagcgaga
     2881 ggatggcgta aaactcgacc gtgatgcaga ggatnnnnnn nnnnatgatg cccttcgccc
     2941 agtcgtccgg acgcttggcc atcaggttga cggcggtagc ggccacgcgg ccctgcgccg
     3001 gagcggagat agcggcgccg gtcgccatcg ggaggcacgc gcacgcaacg cgcagaccgt
     3061 caacgacggt gaggctggtc ggatcgacgt tgccgccgaa cacgcccatc tggagcagcg
     3121 caaagaacca gacgacgagg ccgtacagtc cctgggtgcc ggggatgacc tgaaggatca
     3181 ggactttgcc gaagtaagag ggatcttcgc tgagaaggcc ggtaccggcc tcaccggcaa
     3241 ggccgcagcc cttggcagaa ccggcgcagc agagaccgca cgcgagggac gcccccagga
     3301 aaccgatggc gagaccgccg aagctctcaa agaatgcagt gatcattgtt ttactcctcc
     3361 ttgatatcta catatttcgt ttgaattttc aaaggacgga aagccttgcc gccgtcccgg
     3421 taccacttgc cgaagaactc gaggaactgc agtcgcagcg tgtgcacgaa gcagccgatc
     3481 aggttcagtg caaagttcag cgcatggccg atgcagaaca cgagcacgaa gaggatcgtg
     3541 ccgcccacgg tcatgccgtt ggcgttgccg agcgagccga gctggttgaa tacgctcgca
     3601 ataacgctgc cggcgagcat cagcgccatc agacgcgagt aagagagcag atcgcccaga
     3661 tacccggtcg cgccgttgta taccgcgccg aaaatgccgg tgatctttcc gaagcccttg
     3721 ccggtgatga gctgtccgag cacgagcatg ccgatgccga cgaagagaac gatcttcgtt
     3781 ccgccggtca ccatgacgag aatgcccgcg atcatcaccc accagcacag atcgttgagc
     3841 aaagcgtccg cccactcgcc gtccttgagc ttgagataga tgccgatgcc cgtgccgagc
     3901 gccagatgga tcgcgccgag gcagatcgcc gcgatgagga catacagcgg gttgttgccc
     3961 tcgagcagat tgagcggcag atcgatcgcg atgctgccga tatggatcgt gccgaggtgc
     4021 ggtacgatcg accagtccct gccgaaccaa ccgccgatga cggagacaac gtcgccgaag
     4081 aagccgccgg tcaggaagcc tatgatgaat gtggaaatac cgcattcgat cagcagcccc
     4141 gcggcgtttt tcatgcttcc cttcggcttt gccttccaga gaaagagcag tcccgcgatc
     4201 agcaatatca ggccgtacgc catatccgcg aacatgatgc cgaagaacag cgcgaaaaac
     4261 ggcatgataa aggggttggg gtcaagtccg ttgtacgccg ggagagaata catctccgtg
     4321 acgatgttgt acggacgggt cagcgcgtta ttgcgcagct ggatggggac ctcctccggg
     4381 tgctccggat cgggctcctc cgtttcccag gcgcagcagt atttcgagag agtctcgccg
     4441 agttttttct cctccggcgc ggtgagccag ccttcaaaat agaaggtgct gtccgactgc
     4501 aggagctttt cggccgtctc ttcacgcgca atgcgcgtcg agagcgtgtc cgcgccgagc
     4561 tgcagcgcgt cgcggtgcgc cgcgtaggag gcaagctctt cgatcttctt ggccgcctgt
     4621 gccgtcagct cctcactgcg gcggagcgcg gcgctataga cctgctccgg cgttccggtg
     4681 ccttccggca gagaaatggc cgaaaagccg agcggccgca gcgctgcggc gatcgcttcg
     4741 cgatcctccc ggcggaagag cagcgataca taccggctgg ttttgtcctc ggaaacgaga
     4801 tcaaagctga cctccagctc cgagagcgcg gcgtttactt cctccatggg gacagcggcg
     4861 ggaagtgtgc cgagctgcat ttcgcagctt tgcgtctcca tgcgctcaag cgggacgttc
     4921 agcgccgtcc acggagtcat ggccnnnnnn nnnncctccg ccgaggcgcg ttcccggctt
     4981 gcgcaggtga gaaggctgcg cgcctccgca tccagcgaga ggatctctcc cgccagagcg
     5041 agatcggcgg ccaaagcgct ctcgtcgagc agcttggcgc attcgacctc cggcttcggc
     5101 gtaagcaggc cctttttggc aggggcgtgc ttgtccagca gccgcagcgc gttgacgatt
     5161 gttgtctgtt ccgagcgctt ctgccagacc tttccgccgt ccggacgccg gaaggccgcc
     5221 agctcctccc ggtccggccg gccggccgga tccgacacct ccacgcagcc gagaagcatc
     5281 agctcacgnn nnnnnnnnct tccagacggt cctccgcgtg actgcgcaga gaatccatct
     5341 cctcgcgcgc cgcttcccgg atcttttcac tttccgcagc gctcttttcc tctgcgctct
     5401 tcgcgagagt ccagagctct gccgcagctc tttctgcggc ttccttcaca gctgcggtcc
     5461 cctctctgtc ggctttcgcg gcaatttcct ttgctttctg cgccgcttcc gccttttttc
     5521 gggctgcatc ctgttccgcc tgctctacgg caagaattgc ttccagtgcc attttttgaa
     5581 acacctcccg tcaaagccaa attttctgac atcgtaaatg tacacctttt tttcatcaaa
     5641 ttcaagtgat aatgtcgtta aattgtcacc cgaaaagtct tgtgcgcagc agtttttccg
     5701 ttttaaccaa atgaagactt ctgtgtttgg gcaagttgca ttttatgctt gcaaaatcga
     5761 gaagaaacta tataatatta tataataaac aaattgtgcc catcgaacgg gcacaaaaaa
     5821 gccccattct gcacccataa aaggagactc tttcatggca gagcgataca cccgacatat
     5881 gaacgaccgg cgggagggcc gccagctcta tacgcttccc gcctacgccc gcatccagcc
     5941 gtacatcctc cgccgccgga gcgacgccgc ctgcttcctc tcggacagcg tggaggtcac
     6001 gggcgtggag aaatggctgc gggaaaagcg cgccggcggc tgggcgaacc ttggctttct
     6061 gcatctgatc atcgcggctt acgtccgcac ggtgtccatg cgtccgggca tcaaccggtt
     6121 cgtatccgga cggcgcatct atgcccgcaa cgacattgaa gtcgttctcc ccgtcaagcg
     6181 cagcgcctcc gtcaccgcga cggagacgag catcaaggtc tgcttcgcgc cgaccgacac
     6241 ggtgttcgac gtttaccgca agctcaccga ggccacggac gaggtccgcg ccgacatttc
     6301 cctgagcgcc ccggaaaagc tcgccaacac gctcatgcgc ttcccccgcg ttctgctgcg
     6361 catggcgatg ggtattttcc gtctgatgga ttattttgac tggctgccgc gcacctggct
     6421 ggatgtcagc ccgttccacg gcagcgtaac gatgctcgat ctcggcagct tcggcgtccc
     6481 gcccgcggat ccgcaccttg ccgatttcgg caacatgccc tgcgccgttt cgttcggcgc
     6541 gcggcgcaag gtgaacgagg cggacggcac cgcctccggc gtcgagcggc actatgtcga
     6601 tctgcgcatc gcctgcgacg agcgcatcgc cgacagctat tactttgcca gcgctctcaa
     6661 atgcctgaaa tatttcctgc gcaaccccca ccttctggag ctggctccgg agtctgtgga
     6721 ggaggacgtg aactgatttt tttcacgggt gggaagcttt ttccgcccga tttccctccg
     6781 ctgccggatt tttccgcgaa aaatgctgcg gcgggggctt gcaattttcc tgtcttgtgg
     6841 cataatatta tgttaacagg tctatctgta tttataaggt acataccggg agatactcca
     6901 tgaaacgaaa gatcctttct ctgatcctct gctgcgcgct gctcttctct ctcgccgcct
     6961 gcggcaagac cgaagcgccg cagccgacgg caagcgccgg aacggagacc gctccgaaca
     7021 gcgaaaaccc cgccgcggcg gagggcattt cctatgccga tgcctacgcg cagtacaccg
     7081 cgatctacgg cgcgctgctc aacgccgtgc agcagcggct cgacacgcac gacgccagac
     7141 tccaggccgc gtatccggac agctactaca tgaactccga ttaccttctg ctcgtgtacg
     7201 ttccgttcac gacggcgtat ccgtcgctcg ggcgcgagct gcgcgccgac aatctggacg
     7261 cagcgcagac gtcgctccgc gaaacgttcc ccgacgccgt gctcacgatg accgcgccgg
     7321 gacgcttcga ggcggtatac acctatgtgg acaagacctc cggcacggag gtgcagcgcg
     7381 agggcaaatg ccagtgggac tgcgacgggc tgaccggtgc gatccgcgtc cgcgcgtaca
     7441 ttgacggcga gctgaccgag tttaccgagt ttgtccccct cggcggcagc cggtacctgc
     7501 tctacaccgc gaccgacaag gcgatcgtcg agtacatcga gggcgagatc accgcgctct
     7561 ggcacgccca ccgcatcagc gagccggcgc agggcctgtt ccccggcgac atgcggctta
     7621 tgagccttgc cgagcaggac ttcttccccg gcgagggcgc ttccttctcc ggcacggact
     7681 ggatcaccgg cgatgccgac gcgcagtatg tcatcacgct gcaaaacggc gaaatggttt
     7741 ataccggcaa gatctcgcag gatctcgttg atcaggacgg caaccgcacc ggcatcgtct
     7801 ggcaggacat cgcgccgatc acgcttcttg cgtgaacgga tcgccccgtc catgcgtcat
     7861 tattataggt aaaccgatac gaacgatttt ccaaactacg cgacccccgc gcggggtcaa
     7921 cggagggaga aaactgatat gaaaaaacgc cttctttccc tgctgctgac cgtatgtctc
     7981 gttgcagcgc tcttcaccgg tctcaccggc acggcgaacg ccgccaacga caaggtcgtg
     8041 ggctatctta cgagctatac tctcaaagcg ggcgacacga tctacggtgt gtgtgaagcg
     8101 aaaaagatcg acttcaacgc caacctcgcc atgatcggca agatcaacgg catcacgaac
     8161 tacaactaca tgatgcccgg caaggttctc tggctgccga cgaagacggc cacgacttcg
     8221 gagtcgtatt attctctcct ctcgcacacg ctcgtcgccg gggagacgcc tgccgcgctc
     8281 tgccagagct acggcattga ctacaacgcc aactacaagc tgctcgccgc gctcaacaac
     8341 aatctcaacg tgttcatggc aggccagcag ttcattctgc ctctgtacgt aacgcctgcc
     8401 ggcgccgcga cgccgactcc ggcgccgacc tccggcacct ctgccgcgcc gaccgccacg
     8461 cccaagcccg gcacgacgcc cgccccgacg gcgaacgttc ccaccggcga cacggtgagc
     8521 tattatctcg cgcagcacac gctgcagtac ggcgagaccg tttccggcgt ctgcgccgcg
     8581 ctcggcgtgg acttctcctc gaacgatgcg acgatccgca agatcaacaa catcaccaac
     8641 tacaactata tgatgcccgg aaaggtcatc ctcattccga cgaaaacggt cccgggttcc
     8701 ggttcttact acaagatcat ggcgcacaag atcgtctccg gcgacacgct ctacgcgctc
     8761 tgcgcctcct acggtctgga ttactacgcc tattccaagc tgatctccct gctcaacccc
     8821 aacgcggcct attataacct gctccccggc aacattctct atatgccgca gtatgtcgcc
     8881 gcctctacaa cggtgaagcc tgccgcaacg cccgcgccgg ccgcctctgc cggtacgacg
     8941 gccgccccgg ctgcgacgcc tgctccgagc gcgtctgccg gcacaacggc ttccgctgcg
     9001 ccgagcgcca cagccgccgc ttccgccaag cccactacga cgcccaacat ccccgccgag
     9061 gatacgctca gctatctcat catcccgcac aagatccagg ccggcgacac cgtcatgagt
     9121 atctgcgata aatacaaagt ggacttcgct gcgaacgacg ccgcgatcca gcgcctgaac
     9181 ccaagcgtga gctacagcta cctgctcccc ggcaacatta tcctcatccc ctcgaccacg
     9241 tacccctcct ccggtccgta ctacaagatc atggcacaca agctcgtcgc aggcgacacg
     9301 gtctacggtc tctgcctcac ctacggcctg agctatgaga acaacatcac cttcctgcag
     9361 cggctgaaca accgcgacaa tctcgccacg tactacgtcg gccagacgct ctacatgccg
     9421 ctctacgttg ccggctgagt ctccaatatc cgacaagcaa atgccaaatc gctgcgtcgg
     9481 tgcatcaagc aaataccaaa aaccgctgcg tcggcgcatc tcttcggaat gcgtcggcgc
     9541 ggtttttttc ttccggtctg gaaggaatgc ataaaggccc ccgagcgaaa ctagcagatt
     9601 attacaaaga aagcgaggta gagcagagta accttcaccc gaggggaggg ttttttattc
     9661 atgcaatctt gacaaaagat tttctttctg gcttactgat tatagtgaag ctgaccattc
     9721 tagataagca gctgagaaac ccaaaaggag aacggcgcag gaaggaaacc tgcacggcca
     9781 tttcctgccg gatggcggaa gcggctcgtc agcagacagt gctccggagc atatgacgat
     9841 atcctccgga gactggatca ttacaaggtt tcaccccccc agcagcgcac cccctctctt
     9901 tcccgatatt cgggcgcaca acaaagcaca tctccgcatc ctgctgcaca gcgagtccgt
     9961 tattcaaggc ggccggcggc tcggaaaacg ggcagccggc acagaccacg cgcaacttca
    10021 ggagaaccat gtgtttcaag caacgcaaaa aagaaggagg cattctaaca tgggtaaagg
    10081 caaatacact ctgagaagta tcatcgtccg ctgcgttgtc attatggtcg gcgtcttcat
    10141 cactgcctac ggcgccgaag cgtttgttct ggccaatctg ggttctgacc cggtcacagc
    10201 gtttgtacag ggactcagca aggtgctgcc cttcgtgcat ggcgactttg gcatggccat
    10261 gaacgtattc aacgtcctct gcttcgtcgt catcctgatc gtgtaccgga agggcatcgg
    10321 tgttgccacg gtcctgtaca ccttcacgct gggcaccttc tgctccatga tggagccctg
    10381 gatcatcgct ctcatcggcc ccactccctc tatggtcacc cgcgtcattc tggtcatcac
    10441 cggcacgctg gctctgggcg tcggccttgg cagctatcag gccgccggct acggcgcagg
    10501 cccctctgac ggcttcaacc agttcatggc caagaccctg aagatgaagc tgaagtggtg
    10561 gcgtatgatc ttcgacgcca tcatggtggt aggcgctctg atcatgggcg gcgtcatcca
    10621 tgtggccacg ctggtgggta tgttctgcgt cggccccgtt ttgggtcccg tgctgaacaa
    10681 gctctcgcct ctggtcaaca agtgggcagg caacgaagac ctggcctacg aagaaatctg
    10741 atattccctg tcggatccaa ccacagcccg gagcggctgt ccgctccgga gaaaaaattt
    10801 gcccctgcaa aaaaatcgaa aagttttaga aaggcggtaa tttactatgg ctgaaaccat
    10861 gaagggttat gtgtttaaag acgttaacga tcttcgtctg gaggatcgtc ccattcctac
    10921 cattcaggat ccccgggacg ccatcgtaag ggttgctctg tcctccatct gcgccagtga
    10981 cttccatatc aaggtgggcg ccgtgccctc cgccaacaag gacatcattc tgggtcatga
    11041 gtttgccggt gaagtggttg ccgtgggcga tgcctgcaag aaaatcaagg tgggcgaccg
    11101 cgtcgtcgcc aacgtggaga ccttctgcgg cgagtgcttc ttctgtgaac gcggctatgt
    11161 taacaactgc gtagagggcg gttggtatct gggccacaag atcgacggct gccagacgga
    11221 gtatgtccgc gttcccatcg ccgacaacgg tctggagctg atccccgata atctgacata
    11281 caaagacgtt cttgccgtca gctgcgtgct gccctccggc tattttggcg cggagctggc
    11341 tgagatcaag gaaggggaca ccgtggtggt tctcggctgc ggcccctgcg gctatacttc
    11401 catgatgagc gcccggctgt tcaagcccgc ccgcatcatc gcggtggacc gccatgacga
    11461 gcgcggccag ttcgcgctgg atcacggctg ggccgatgtg ttcatcaatt ccaaaaagga
    11521 ggacgtagag gctcgcgtca aggagctgac cgaaggccgc ggcgccgacg ccgttatcga
    11581 ggccgccggt aaggacggca ccttccagct ggcctggaag atcgcccgtc ctaatgcggt
    11641 catctccctc ctcgccatgt acgaggaaga tcaggttctg cccctgccca gcatgtatgg
    11701 caagaacctc acctggaaat ccggcggcgt agacgcctgc aaggatgctt atctggtgca
    11761 gctgatctcc gaaggaaagc tggatcccac gccgttgttc aataaggaat acaccctcaa
    11821 cgagatcgcc caggtctatg aggactttgc ggtcaaacgc gatggtttga tcaagccggt
    11881 catcaagccc tacgagtact gatcttcttt tctctcccct ttcccagttc tccaaatacc
    11941 cccgcgggag ctcgctgcaa agcgagcttc cgcgggggtg tcttttacgc gcttctccgg
    12001 tttttttgat tctgtatccg ccgttatcgc gcacggcctt tgcgtccgca ccgctgcctg
    12061 ctcttctccg ccgcctgtcg gccgatttcg accgtctgtc gcaaaatagt ggacgttttt
    12121 tctccgatcc cttattatat ggccatggaa ggaaggggat cggatgaaga tcgaaatcca
    12181 aatcgacgag agctgcgacg agccgaagat cgtcgttatg acacacaggg tgaccgagga
    12241 aatcagcgaa atcctgaaaa agctttcgga agatccgtcc aaaatgctgg caggcttccg
    12301 ggacgagcgg gcaagcatcg ccggaaatga gcgtgccttc gcaaaaagct gtgggaagcc
    12361 atcccttcac ggatgagcct cccacagctt tctattggaa tcgatattaa gcaaaggtca
    12421 ttcgccgttg ccgcgcacgg ccttgagcgc ctgcgcgagc tggtccgggc aggacgtcgg
    12481 cttcatgccg cagcggatgc cctcgagccg gtcgatgact tcgttcacat ccatgccctc
    12541 gcagagtttc gcaacgccct gcgtgttgcc gctgcagccg ttgatgaact gcacgttgtg
    12601 cacctttccg tcttccacct cgaagaggat cttgcgggag caggtgcccc gcgtcgtata
    12661 ttcgtgtgtc atgtccttat ctccttatcg aaaaactaat atgcctcgca gagtttgtgc
    12721 ccgcaggcac aaatgagttc ccaatcattt tctccggcgg cgtgtacgtc ggagaaaatt
    12781 cctctcggct cgcaaacgtg cgagcgcagc gagtgcgctg cggcgagccg cagccttttc
    12841 aaacgaaccc tcaacgaagt ggggttcgtt tgagagcgaa gctcaaagca aggtgctgcc
    12901 gttgtagctg acgatgttga catcggaaac gccttcgtta tcgagcagcg cttcgagcga
    12961 atccatctgc ttctggctga gctttgcctc cacgacgagc tcggtctcgg cgagattcat
    13021 cgtgcggctc ttgagcttat atttgccgag cgtgccgagc ttggagcgca ccgcgctctc
    13081 cccctccggc gtatagcgca taacgacaag atacgagttg gtgtgcagct tcccggagag
    13141 cagattgagc gccaggaaca gcgcgccgag cagcagcgcc gcgagaatgg caaccgtgat
    13201 gtaccccgcg ccggtgatga tgccgataac gatgctccag aacatgaaca ccgtgtccat
    13261 cgggtccttg atcgccgtgc ggaagcggac gatggagagc gcgccgacca tgccgagcga
    13321 gagcacgacg ttggagcgca ccgtcaaaat ggcgagcgcc gtgaccatcg agagcatcac
    13381 gagcgagagc gcaaagctct tgctgaagct gacgccgccg taggtcacgc ggtagatcac
    13441 cagcacgaac agacccagcg caaagctcag cagnnnnnnn nnngttttgc ggagcgtcgt
    13501 gagcagcgtg ccgacggaga tcgcggtgaa ttcctcaacg aatttgtttt tgatgagatc
    13561 ggaaatgctc attggttttt acccctcttt attccagaat gtctaagcat ttggtgtact
    13621 tggaaacggc gcagcgctgc tttgtcacgc cctcgagcag cgcgcggacc ggcgcgggaa
    13681 ggaacgcgtc gtacttgacc tccagcaccg cctcgccctc atcgagcgcc ggtacagtga
    13741 gagcgcggtc gtcgaaaagg ttggttttga acggcgcggt gcgaagctcc gtgtcgatcg
    13801 tgacgcggac gttgcccgtc ggatagacga acggataccg gacgtaatcg accagcactg
    13861 cgggcttcca gccgttctgc cgaagccggg caagctctcg tgcgagctct tcggtatcgc
    13921 gcagctcctc gccgccggtg aggaacgcct ccgcaagttc gcgggagatg cgcaccgatt
    13981 ccttgccgga gaagtcgccg tccttgctct tcttttcgag cgtgattcgc gaatcgtcca
    14041 tgttgtaaaa ccggatgcgg tacttggtgc gttcctttac gcccgcgatc ttctcgtgat
    14101 aggcgcggta gtcggtatcg tcaaaataga gactgcggat aaagtagctg ccgtcggctc
    14161 ggacgtgcgg gtcgggccgg agcagggcgc gcagccgata tttcaaaagt tccgcctccc
    14221 gcagggagat gatgtatttg atctcgtggc gtttgatctc tgccatagct ctcccccctt
    14281 acccgttggc cttccccggc gagggggagg cgcattcgcg ccagtcggag ccgatgcgcg
    14341 cgagcgaggc gtccggctcg gtcggcgtga gctccacgcg gtcggcggtg ctgccgatcg
    14401 gcgttgtcaa aatcacgctc tcgcccgcgg agagggcaaa gtccgtgtgc ggctcgccct
    14461 gcgtccggtt tttgccggag gcgaaaatga tgcaatactc gcccggcgcg agcgtaatgt
    14521 ccggaatgcg ccagcgtgcc ggctcctccg gatcgtcgga gagataccag ccgtcgagcg
    14581 atacgctctc cgttccggcg ttataaagct cgatccagtc cggcgaatcg ccgtcggcgt
    14641 cccgcagacc ggcttcgctg cggaggcaga cctcggtgat ctgcaccgca acgtccccga
    14701 agccgcgcga ggcggcaaag gcggcgtagc cctcttcggt gttcgcaaag cccggcgtcg
    14761 gccggttcgt gacgcaccac tcgcccgaaa cgcgggcata cgacgtgtca tccgcgagga
    14821 acggcgtctg gatgcggtca agtgcattgc cgtcggcgtc ggtcagaatg acgctctctc
    14881 cagccttggc gagccggatg gcggcgtagt ctgcgccctc cgtcccgtcg ccggagcacc
    14941 agacgaccgt gaacgcgccc ggtgcgagca gcgttccctc cgggaacgtg tagcgcgcct
    15001 cgcccgcctt gtcggtgagg tgcataccgg agagatcggc ggtctcggag ccggtatttt
    15061 cgatctcgac ccagtcgcag agcgcgccgt tcggcgcggt gaagagcgtt tccgcggcca
    15121 tgatctcgct cagccgcagc gcgcccgtgc cctgtccgga ggcagccaga tacgcggtgt
    15181 atccctcgtc ggtgttcgca tagcccggcg tcggcgcggc ggagacggta aagctaccgt
    15241 cggtctcccg cacaatggac gtattcttcc ggcagcggag cgtttcgatc tcgtcgagca
    15301 ccgtgttggc gctgttcatc agcagaagcg tttccccgcc gagccgccgc agcgaaaacg
    15361 gcgcgtacag cccgccggat gtctccgggt cgcagtagac gacgatgtac ccgtccgcgg
    15421 ggatcaccgt cccgaccggg aaggcgtatt tggcctgcgt cacgtcgtcc gaaaggcggt
    15481 agccggaaac gttgaaaggc ttatcggacg tattgtgtat ctcgatccag tcgcagatgc
    15541 gcccgtcagc gttcggatac gccgtgttcg cgctcatata ttcactgatg cgcagcgggt
    15601 gctccccgct ctgcacgcgg tcgctgcccg cgccgcgcga gagcagcagc gccgcggcca
    15661 gcaccagcgc gaacaccgcg agcgaggcga tgaggatcgc gatctttgtt ttctttttca
    15721 ccggaaccat agccgtgctc cattcaaaga aagaactgtc attcaagttg gattttaccc
    15781 tatttctccg ccgttttcaa cccctcggcg tttatacgat ctggaagaga tagaatttca
    15841 gataatccgt ttccggcacg ccccagagga tcgggtggtc gggactctgc tgccgcgcct
    15901 cgatctggcg gagctgtacg ctcgcgtcgc gcgccgcgtc cgcaagcatg gcgcggaaaa
    15961 gcgtgtcggt cataaagtgc gagcatgagc acgttgccag atacccgccc cgcggcaata
    16021 tctgcatggc gcggcggttg atctcccggt agccgcgccg cgcgttttct accgtcgcgc
    16081 cgcttttcgt aaaggcgggc gggtcgagga tgatgtaatc ccagggcttt tcgccgctct
    16141 ttccgagctc cgtgagaagc tcgaacacat ccgcctgccg gaactccatg cgcccttcga
    16201 gcccgtttct ctcggcattg cgccgcgcct gttcgagtgc ggacgcggac acgtccaccg
    16261 ccgtgacgct ctccgcgccg ccggccgcgc agtggagcgc gaatgcgccg gtgtgcgtga
    16321 agcagtcgag cacgcgccgc ccatgggcca gccgtcccgc cgccgcgcgg ttatatttct
    16381 ggtcgagaaa atagccggtc ttctggccgt ttatgtagtc cacgtcgaaa tagatgccgt
    16441 tttcgcagat ctccgcgtgg ccggaggggt ttgtctccag accgggcgcg gcatacggcc
    16501 ccttgccgca ggtcatgccc tccttttcgc ggatgagcga ttcgttgcgc tcatagatgc
    16561 acgacacgtt cacgccgagc gccgcgagct cctcgcgcag cagcgcatag agcattgcgc
    16621 gccgccgctc gatgccgagc gagagcacct cgctcacgag cacatcgccg tagcggtcca
    16681 ccgtccagcc ggggaagccg tccgcctcgc cgaagatgag gcggcaggcg gcaaagtcct
    16741 cgcccatgac ctgctgccgg taggcgagcg cataccggat acggcggcgg aaaaatgcct
    16801 cgtccatttt gtcgttcgtg ttgcgcgaga gaatgcgcac gcggatgagc gagcggccgt
    16861 tataaaaccc cgcgccctgc cagcggccct tttcggtata tacgtccacg atctccccgt
    16921 ccgggcaggg gctttctttc gtgagtacct ccccggcgaa cacccaggga tgcccgccgc
    16981 gcagagcgcg cgcggccttt tccgtaacgg taacgcttgc aaacggtctt tccatcgtct
    17041 gtcctcctgt gtggtaacgg gtcattatac aatataattt ctcgtttggc gagagaaagt
    17101 tgcggcgtat gaaagaataa gccgctggac atcgatccga tatccaacgg cttatccttt
    17161 gtaaaaaaga ggagaaagag aagaagaaaa agagaaacat gaaacaggcg gtttcttgcg
    17221 aagtttccac cagagcattt gtaattgaac acaatcccca ttatgttcta ataacaaaca
    17281 catttttatt ggttcgccct tttcggttca tatactacca cgtttcgtca aatagcgcaa
    17341 ccatttcttt attaatgttt tgtgaacttt cccgccgtgc gccggagctt gacagcgttt
    17401 tttccgcgtt ttattattat agtgtatctt ctccccgaaa ggcagggcaa agcatgacga
    17461 acattctcgt cgttgaagac aatgccgggc ttcgtcggct gatcggcatc catctccgcc
    17521 gcgccggata tgaggtatcg gaggccgccg acgggcaggc ggcgctcgat cttttggagc
    17581 acagccgcgt gcatctcatc atcaccgacg tgatgatgcc ccggctcgac ggctacgggc
    17641 tcatccgcga gctgcgcgaa gcgggcgaca cgacgccggt gctgatggta acggtaaaga
    17701 actcctttga cgatatgcgc accggctttc acgccggggc ggacgactat ctcacaaagc
    17761 ccgtgaattt tgaggagctg ctgctgcgcg tgggggcgct gctgcgccgc tgcggcatcc
    17821 agcactccgg cgagatcgcc gtgggcgggt gcacgctgca cgccgacacg ctgctcgtca
    17881 ccggcgaggg gttctcctat gagctgcgcc cgcgggagtt tgcgctgctg caaacgctgc
    17941 tcgccgcgcc cacgaagatc ttcacgcgcc agatgctcat ggacgagctc tgggggtacg
    18001 acagcgagtc tgacccgcgc accgtggaca cgcacatccg ccgtctgcgc gaaaagctcg
    18061 ccggcgttcc ggattttgag atccagaccg tccgcggtct cggctaccgg gcggtgatcc
    18121 gatgaggcgc gtccgctcgc ggctgacgat cctgctgctg atcctcgtga tcgtcacgtc
    18181 gcttttctct ctcaccttcg gcgcgctcgt gcgaaacggc atcatcttcc agcagcgcga
    18241 caacatacgc tatcttgttt tcggctacgc cgccaaggac atactgcttc tgctcgtcgc
    18301 cgtggcggcc gtcgcggtgc tcatcacggt cacatcccgc tcgacgacga atcccatccg
    18361 cgagctctcc cgcgccgcgc gcgagatcgc cgggggcaat tacgacgtca ccgtgccgga
    18421 gcgcgaccgt gtggaggaat tcggcgagct tgagcgcaac ttcaatctta tgacggcgga
    18481 gctccgctcc aacgaatatc tgcgcaagga cttcatttcg agcgtatccc accagctcaa
    18541 aacgccgctc tccattctca acggctatgc ccagcttctc gccgaaggcg gtctttccga
    18601 gaccgaggag cgcgagtaca gccagtacat cgcgcaggag tctgaccggc tcgtcgggct
    18661 catcgatgac atgctgcgcc tctcgcgcat cgaccaccgg gaaatccatc gaaaggcgga
    18721 gatcttcccg ctcggcgagc agctccggcg cgccgtactg cagctcgagc cgcgctggac
    18781 ggaggccggg ctttccgtct ccgccgacat cccggatctc gactacgtcg gcgacagcga
    18841 gcttctctat caggtgtggg tgaatctgct ggaaaacgcc gtgaagttca cgccgcccgg
    18901 cgggcgcatc ggcatctgtc ttgccgccgc agcggatacc gtcaccgtga cggtgtggga
    18961 caccggctgc ggcatggacg cggaaacgct cccgcgcatt tttgagcagt tctatcaggg
    19021 cgacaccgac cgccgcgccg acggctgcgg actgggtctc acgctctgca agcgcatcgt
    19081 ggagcagcac ggcggaacga tctccgcgga gagccgtccc ggagaaggca gccgcttcag
    19141 cgtcgtgctg ccggcaaagg ccgcaaaatt catgtaaaga gactccatag caggggaatg
    19201 tttaacgtta agttcacatt cccctgctat gttaatatcc agatttttat atgttagcga
    19261 ggcaacaccg tgaaagctat caatcccgga cactttcttc tgacctgccg gctggacaac
    19321 tgggtgcatc tgctggaaga gaaccacttc catatcgccc gcgaccggct gccgcaggcg
    19381 ctgtacattt ccgcaacggc gctcgcgctc gcgcccgcgg cggcggcgga atcgctcatt
    19441 taccgcaagc gcatccgtga aacgaagatc gaaaaggacc cgatcttcat tctcggccac
    19501 tggcgcagcg gcacaacgta tctgcagaat gtcctctcgc gcgacgagca gtttggctgg
    19561 ttcgatcccg tgaacacgat cggcctgccc tactcgctgc tgctcggccg gctcatccag
    19621 ccgccgatcg aaaagggcat ccagaacggc cgcccgcagg ataacgtgca gtactcgctc
    19681 gatctcccaa tggaggagac gttcggcgtg ctgacgatct cgccgtattc gatcatccac
    19741 atgatcgcgt tcccggaaaa ttataaaaag tacatcgaag gcgcgttcgt gagcgacctt
    19801 cccgtggagg aactgcgcaa atgggagcgc agctacgatt acgccgtccg caagctcacc
    19861 tacatcaaaa agggcaagca gctcattctc aaaagcccgg acaacactgc gcgcatccgc
    19921 gagctgtggg gcatgtatcc ggacgcgcgg ttcatcaaca tccaccgcga cccttacaag
    19981 accgtccgct ccacgatcca catgttccgc accgagatgg actcgctccg gctgaccgaa
    20041 gagccggata acatcgacga gctcatcgaa aacacggtca tcgatatttt cgagcgcatg
    20101 taccgcgagc ttttcgagct tgagggcttt ttcccgaaga accggtacgt ggacatcgcg
    20161 tacaccgatt tctgccgcgc cccggtcgat acgctgcggg atatttaccg gcgtctggag
    20221 ctctccggct ttgaggcggc cgcgccgcgc tttcaggcgt atgtcgattc gcagcgcgga
    20281 taccagaaaa ataaattcga catttcgccc cggctcgtgc gcaagatcaa cgcgaagctg
    20341 ggcttctaca tggaacacta cggctacgaa atgcgggagg ttgaggaaga atgagagatc
    20401 atctcgataa actggaaagc aaaagcgtgc atatcctgcg cgaggcttac gccgcgttcg
    20461 gcaatctctg catgctctgg agcatcggca aggactccac ggtccttctc tggctcgcgc
    20521 gcaaggcgtt ttacggccac gtccccttcc cgctcgtcca catcgacaca cattataaaa
    20581 ttcccgaaat gatcgagtac cgcgacaagc tcgccatgga gtacggtctt tacatggtct
    20641 acggtgagaa ccgcgaagcg cttgaaaacc acgaaacgtt cccggacggg aagtgctctc
    20701 gcgtcgagtg ctgccgccgg ctcaaaaccg aggcgctcac ccacacgctc gacggcacct
    20761 ggccgcgcta ccggctcaat cttcagaccg ggaaatacga gcgcgacacg aacaccgacg
    20821 cctacaccgg cgtcatcgtc ggcgcgcgcg ccgatgaaga gggctcgcgc agcaaggagc
    20881 gctatttctc cccgcgcgac aagcacaacg actgggatct ggacgaccag ccgcccgagt
    20941 tctggaacca gttcaaaacc gactttgccc ccggcacgca tgtgcgcatc catcccctgc
    21001 tcgactggac ggagctgaat gtctgggaat atatcgagcg cgagcacatc cccaccgtgc
    21061 cgctctacta taatcagggc gacggcacgc gctaccgctc gctcggctgc gcgccgtgca
    21121 caaagccgat ccggtccgag agccggaacg ttcacgagat cattgaggag ctgcgcaccg
    21181 gcgctctttc caacattgcc gagcgcagcg gccgcgccca ggacgccgag gacggcggcg
    21241 ggctcgaaac gcttcgccgg ggagggtaca tgtaatggaa gaaacaagaa acgtctggga
    21301 aaacgaggag cttttgaata tcgtcaccgt cggccacgtc gatcacggca aatccaccgt
    21361 gattggccgt ctcctcgccg acgccggagc gctgccgaag ggcaagctcg aatccatacg
    21421 ggaaaactgc cgccgcaact cgaagccgtt cgagtacgcc ttcctgctcg acgcgctgca
    21481 caacgagcag gatcagggca tcacgatcga cacggcgcgc tgcttcttca agtctgacaa
    21541 gcgccggtac atcatcatcg acgcgcccgg ccacattgaa tttctcaaaa acatggtcac
    21601 cggcgcttcc cgcgccgagg ccgcgctcat ggtgatcgac gccgcgcgcg gcgtggagga
    21661 aaacacccgc cgtcacgggt actttctctc catgctcggc atccgtcagg tggcggtgct
    21721 cgtgaacaaa atggatctcg tcggctactc cgaggaggtc taccgctcca tcgtcacgga
    21781 gttcaccgaa tttctcgcca aaatcaacat ctcgcccgcg gcattcatcc ccgtgagcgg
    21841 catggagggc gacaacatcg ccatccgcag cgagcgcacg ccgtggtact tcggccccac
    21901 ggtgctcgag cagatggacg ccttcgtccc cgtgccgagc ccggaaaacc gcccgctgcg
    21961 catgccggtg cagggcgttt acaaattcac cgagggcggc gacgagcgcc gcatcgtggc
    22021 cggaacgctc gaggccggaa gtctccgtgc gggcgataag atcgtattct acccctccgg
    22081 gaagagaacg acggtcgcct cgctcgaggt attttccgcg cccacgccgg agaaattcat
    22141 tgccggggac gccgccggct ttacgatgac cgagcagatc ttcgtccgcc gcggcgagat
    22201 cgcgtgcttg gagagcgaag aagcgccttt cgtcggcgtg cggctgcgcg cgaacgtgtt
    22261 ctggctcggc aaggagccgc tgcgccccgg caagcgctat ttcttcaagt gcggcactgc
    22321 caaggtggaa gcgcggctcg agagcatcct ccgcgtcgta gacgcctcgg agctggacag
    22381 tctggagcgc agcgaggtgc agaaaaacga agtcgccgag gtgatcctcc ggctcgaccg
    22441 cccgacggcg ttcgacaccg ccgactccgg ctgcgacggt acgcgtcggt ttgttatcgt
    22501 ggacgattac gagatcgcgg gcggcggcat cgtcactgag gcgctgctct ccgaggatta
    22561 cgataagcgc aacatccgct ggtcggccga ggccatgacc ggcgccgagc gcgagcagct
    22621 caccgggcgg cgcgggctcg tcgtatggat gaccgggctt tccggctccg gcaaaacgac
    22681 gatcgcgcag gaggtcgagc ggcggctctg cgaaaagggc gttgccgcct acatgctcga
    22741 cggcgacaag ctgcgccgcg gtctctgcgg cgatcttggc ttctcggacg acgaccggat
    22801 cgaaaacatc cgccgcgccg ccgaggtcgc gggactcttc cgcgacgcgg ggctcgtgac
    22861 gctctgcacg ttcatctccc cgttcgccgc cggacgcgcc gaggcgcgcc gcatcgccga
    22921 cgggcgcttt ctcgaagtct gggtgcgggc cggtctggag acctgcaagg cgcgcgaccc
    22981 gaaaaagctc tatcagaaag ctctcgaggg aaagatccgc tcgttcacgg gcatagactc
    23041 tccgtatgag gagccgaccg acgccgagct tgtgctggat accgaggttc tcagcgaaga
    23101 agaatgcgtc aaccggctca ccgacgccgt ttttgactat ctggaaggcg gagacacact
    23161 gtgaactggg gtaaatttct tcttttcatc gtcatcggct tcgccgcgca gatcattgac
    23221 ggcacgctcg gcatggccta cggcgtgagc tgccggacgt ttctcaaaac cgtggcgggg
    23281 cttccctccg cgctcgcgag cgcggtcgtc catgtggcgg aggtcccgac gacgctcgcg
    23341 tccggtctct cccatatcaa gctcaaaaac gtggataagt cgctcttttt ccatctggcc
    23401 atccccggcg tgatcggcgg cgtggcgggc gcgtggttcc tctcgagcgc cggcgacgcg
    23461 ctcgagccgt tcatcgacgc ctatctcatc atcatgggcg ctgtcattct gcgcaaggcg
    23521 ttcctgaaaa gtcagaagga acgccacatc gggaagggca tttacccgct cggtctcgcc
    23581 ggaggctttc ttgacgccac cggcggcgga ggctggggcc cggtcgtaac gtctacgatg
    23641 ctcgccgtcg gccacgacgt gaaaaagacg atcggcacgg tgaataccgc cgaatttctc
    23701 gtaacgctcg cggagacgac cacgttcgtc gttctcatcc gcgattttac gtcctactgg
    23761 cagacgatcc tcggtctcat catcggcggg gtgtgcgcag ctcccctcgc ggcgtggatc
    23821 tgcaagaaga tccccgttcg cccgctgctc gccgtcgtcg gcatcctcat catcgtgctc
    23881 aatctttaca gtctccttca ctatctggct gtattttaaa aaggcggtat caccatgaca
    23941 cagatgtccg cgcggcagcg ctttgcctcc gtccgcgccc ggctcatgga gctggagctt
    24001 tgggacgctg attcgctcgt gatgaccgcg cccggcgccg tttgcagcgc cgccggagag
    24061 ccggtcgaag cggcgctgtt ctccgccgtt tccgcaccca tggcagtttt gaccgccggg
    24121 cggtacgccc gtgaatttgt caaagctcct cacgcgcttt gcgccgagac cgacgacgcc
    24181 gcgcagatgt tcggcgtgtg cgtgccggta agaagtgcgc cgcggcgcgg cgataccgcg
    24241 tgcatcgttc cgaaccgcgg ctttgtcgtg acggcgcggt ttgaaaacga gctcatcgcc
    24301 gcgtgtatcc tgctcgaaaa gctgtgcatg acggcgcggc tctcccctcg catcgggacg
    24361 ccgaagccgc tcgccgcgcc gctctgcctt atagagcacg cggtctatac gaaaaaatac
    24421 tcccgtccgt cgctcgccgc ggcgcagggg gaagaagccg cgccggtggc acaggacgtt
    24481 tccgatctcg cgcggcgcga gtcggtcatt gtctacggga aaaagctcgt ggaaaaccgc
    24541 ctcattcagg cgacgtgggg gaacgtttcg gcgcgtatcg atgccgatcg ttttctcatc
    24601 acgccgtccg gcgtggacta tgaccgcatc cgtccggagg atattgtgga gatccgcatt
    24661 gccgacggca gctttgccgc cgggcagcgc ccttccagcg agcgggagat gcaccggcgc
    24721 atctatgccg cgcgcggcga cgtgcgcgcc gtgatccaca cgcactccgc ggcgctgcaa
    24781 acgttcgccg cgtgccggga gagtctgcgc acgccggaga tcgtcgcccc ctgcgccgcc
    24841 tacggcgtga gcggctcgaa gaagctcgcg gagagcgcgt ccgaggtgct gcgctctcac
    24901 gatctctgca tcatggcaaa tcacggcttt accgcggtgg cagcggatct tgaaacggcg
    24961 ctctctcgcg ccgtagccgc cgaagaggcc gcaaagcgga tactggaatc ggaataaaaa
    25021 tcggcagcgc cgcattctgc ggcgctgccg aaaatatagg agggcggact atgggagaac
    25081 attttgcgcg gggccggtcg gcggagatat tccgcacgga ggccggaaag atcttgaagc
    25141 tctttttccc cgactatccg agggagtatg ccgaaaagga atacaaaaac acgaagatcg
    25201 ccgcatctct cggctgcact gggatgcagg tctacgatat ggtcgagcgc agcgggcgct
    25261 tcggcttcgt gatggactgc atcgacggcg tttcgcaaaa cgacatgccg gggaaaaagc
    25321 cggcgtatct ttttgaggcc ggcaaagacc ttgcccgctg tcacgtccgc gtgcagaagg
    25381 cgcacacgcg cgagcttgac gacgtgcgtg aggtatgtct cggtattctc ggcgaggact
    25441 gtatggcgtt tctgacaccg gaggaaaaat cgcgcgccgc cggatatctc cgctctctcc
    25501 cggacgacga cacggtgctc catctcgact ttcacaccgg caatgtcctc gtggacaagt
    25561 ccggcgagtg caagatcatc gactggatga ccgccgcgcg cggcaatcgc gccgtggagg
    25621 aagcgctcat ggagttcttc ttctcggagg ccgagctgtt cccggaggcg agcaaagcaa
    25681 aaattgccct cttttccgcc atccgcggct ccatcgggaa gggctttttc aaggagtacc
    25741 agaagctctc gccgctttcc gcggaggaga tcgaccgcta ccgtctcgcg gcgctcatcc
    25801 tgcgccgtca ctggaacatc gcgtttgaag aggcgtatct cacaaaaacc atccgcgcgc
    25861 tgatcgaaaa atacggcgcg gaagaaaagg agacgatcga atgaaagtcg tatgcatctg
    25921 tgacgccggt gtgccggaaa tgctcatgca gtacatgaaa aagctccccg gatgcgacgt
    25981 gacgctctat accgaaaaga cgctcacgga catcaagtcc ctcacgacgt gcgagcgtac
    26041 cgccgagctt cgcggcgcgg aggcgtgccc tgtgagcgcg gaaatgctgg aagccgtccg
    26101 cgaggcggag atccttgtaa cgcacgttgc gcccgtgaac cgtgcggtgc tcgacgccgc
    26161 gccgaacgta aagatcgtca gcgtgctgcg caccggcacg gaaaatgtca atcttgacct
    26221 ctgccgcgag cgcggcatcg ccgtatacaa cgccgagggg cgcaactcca ccgccgtcgc
    26281 cgatctcgtc gtggcgctca tgctctgcga aatgcgcaac atcgcccgcg cccacggcgc
    26341 gctcgtgcag ggcaaatggg tcaaaatgtt cccgaatatc atgatgaacc gcgatatgtg
    26401 ccgctgcacc gtcggcatca tcggcgtcgg caagatcggc acgatggtcg ccgcgcggct
    26461 caagggcttc ggctgccgca ttctcggctg cgacattttc ctctcccccg aggagatcga
    26521 aaagcgcggc tgcgagccgg tatcaaagga agagctgctg cggcagtccg acttcatcac
    26581 catccacatg cgccatgagg ccgggcagag cgctctcatc ggcgcggacg agcttgccat
    26641 gatgaagaag aacgccattc tcgtcaactg cgcccgcgca gggctcgtgg acacagcggc
    26701 gctcgtcgag gcgctgcgcg cccaccgcat cggcggcgcg gcgatcgacg tgttcgacga
    26761 agagccgctc tcgccggact ctcccctgct cacgctcgac aacgtcacgc tcacgccgca
    26821 cattgccggc acgaccatag acgcctttgc caattccgtg gagatcatgc tccgccagct
    26881 ctcactcatt ctcgccggca aggacgcgcc caaccgcgtc gtctgaccgc cgcccatacg
    26941 caaaaccgcc gaaggaaaaa tccttcggcg gttttgcgta tgcggttttc agctgtttat
    27001 gatccgctcc gtggcggcgc gggcatgggc gacgatgtcc tccggcgatt cgccgacgtg
    27061 gtaaacgccg ttctcataga ggatcttgcc gtttaccatc gtgaggagca cgtttgagtt
    27121 cgagccgctg taaacaaggt ttttcacggt gttgtgctcg ggctgcatgt ttgcctggtg
    27181 caggtcgatc acgacgagat cggcgagctt gccgggggcg atgcagtcgg cgtcgatgcg
    27241 catggcgcgc gcgcccgtag agcacgcggc gcggagaatt tcgcgcgcgt cgcacgaggc
    27301 ggcgtcctcc tcccggagct tctgcagcac cgcggcgaga tacatttcgc ggaacatgtt
    27361 gagcgcgttg ttgctcgccg gaccgtccgt gccgatggca agagggatct tccggcgcag
    27421 aaattcgctt accggcgcga tcccgctcgc gagctttgcg ttcgaggccg ggcacgccac
    27481 ggcgtacaga ccgcggcggg caaaaatgtc catgtcggtc tcatcgaacc acacgcagtg
    27541 gaagccgccg ccgccatgct cgaagaggcc gttttcctcg aaaagctgcg tcggcgtgag
    27601 gccgtggcgc tcgatgcact cttctacctc gcgcttcgtc tccgaattgt gggtgaaaaa
    27661 cggcattttg tactcgtcca ggagcatttt cataaacatg agcagatcga gattggccgt
    27721 atactcggcg tggatgccgg ggatgtagga gatgagcggg tggagattgt tgaacttgat
    27781 cgtgtccatc tcgccgaccg tccagtcgtc ccccgcggcg agcgcgccgc agagcaccat
    27841 gcggaagccg cagtcaatgg acgcctgcgc gaaagcgtcg cgcttatagt acatgtcgaa
    27901 cgcgctcgtg atgccgccct tgagatattc gagaatcgcg agcttcgtga gagcgtaaat
    27961 atcgtccggc gtgagcttgg cctccagcgg gaacaccttg tcgaaaagcc agctctgcag
    28021 cggcagatcg tcggcgtagg agcgcaggaa cgtcatcgcg ctgtgcgcgt gggcgttctt
    28081 gaagccgggc atcaggaggt tgcccttgag gtcgatctcg cgctcaaacg gcgggtgcgc
    28141 gccgatggca ggcccgacat agaggatcat cgtttcctcg acccagacct cccagtcgga
    28201 gatctcaaat ccgccgctga gcgaaaggag cttgccgtta taaaagcgga tcatgccttc
    28261 tcctccgcat gcatggcggc gatggaatcc cacatgagct ttttgaactt cggcgcggcg
    28321 gcgttggccg cttcaatgac ctcgtcgccg tcaagcgggc ggtcggagag accggcggcg
    28381 agatttgcca cgcacgaaac gccgcacacg cgcataccgg cgtggttggc cgcgatgacc
    28441 tccacgaccg tggacatgcc cacggcgtcc gcgcccatcg cgcgggaggc gcggatatcg
    28501 gcgggcgact cgtagctcgg gccggtgaac tggatataca cgccctcctt gagatcgatg
    28561 ccgtccttct tcgccgtttc gcgcagaatg gcgcgcaggc ccttatcgta ggcgttcgac
    28621 atatccggga agcggacgcc gagctcgtcg atgttcgcgc cgatgagcgg gttggggatg
    28681 agcgcactga tgtgatcggt gatgctcata agcgttccgg cggtgagatc gccgcggatg
    28741 ccgcccgagg cgttcgtgac gaagaggatc ttcgcgccca tggccttcat aaggcgcacg
    28801 ggcagcacaa cgtcgtgcgg cgtgtagcct tcgtagaggt gcacgcgtcc ctgcatgacg
    28861 acgacgggcg tttcctccac atagccgaac acgaaccgtc cggcgtggcc gggagcggtg
    28921 gacaccggga agccggggat ctcactgtac ggcacaacgg cggtctgctt caccatgtcg
    28981 gcaaagccgc cgagaccgga gccgagcacg acggcgacct ccgggacaaa gtcggtgtgg
    29041 cgccgcaccg cggcgagaca gttctggatg cgttccattt cgggatgcat aaaagagcct
    29101 ccttatgttg ttattctctg ttgtttattc ttcggtgaga tcggcgaact gtgcgccgat
    29161 atagcctgcc agagcttcgg gatcgatccc gatctgcgcg ccgagccgtc cggcgctgca
    29221 gaaaaaggcg tccgtcaaaa gcgccgtttc gtcgatccag gtggaaaacg gttttttcat
    29281 ccccaccggg gagcagccgc cgtggacata gcccgtgagc ggcagcagct ttttctgcgg
    29341 cagcatggcg atgctcttca cgcccgccgc cttcgccgcc tttttgagcg agagcgtttc
    29401 cggcgcgggg atgacgaaca cgaagtgttc gccggtattt gcctccgcca caagcgtttt
    29461 gaaaacgcac tccgcgctct ggccgagcgc ggcggccacc tccgtgccgg aggcgagcga
    29521 ggcgtcgtaa taacgcggcg tatacgcaat gcccgcctga tcgagcaggc gcatcacgtt
    29581 cgttttttcg tttgccttat ccgattttgc catgaaagta ttcctcgatt tgttcacggg
    29641 aagcaagaag gctcccctgg atcatgcccg acgcgccgcc gccccgtccg gagagcgcgg
    29701 cgttgatgct tcgcgccgcg gcgcgaaggt cgaccgtttc cgacgccatc acataccggt
    29761 atcccccgtc ctccgcgcag agcgcggcgc agacgcccgt gcagcgcgtc tttcctccct
    29821 cggcaagggc gcgcagcgcg gcgggcgaaa ggctctcttc aaaaaagagc agatttccct
    29881 cgcacggctc cattgcggcg agccgcgcct cgacataccg ccgctccgcg gcggcacggg
    29941 cgtacttctc cgcttccagc tcgccgagca cgcgctcggc ggcggcgcaa agggcgttct
    30001 gcggcgcgga aaagagacgg gagagcgctt cggtctcggc ggcgcagagc gcggcgtcct
    30061 cataggccgc gcgcccggcg agcatcgtaa agcgcacgcc gccgcggtga cgcatccggt
    30121 ccttcaagcg gatgaggccg atctcgccgg tgaagctcac atgcggcgcg cagcacgcgc
    30181 aaagatccac ccccggcacc tccacgatgc gcaccggcat cgcgagcgcc tttttctgcc
    30241 ggaacggcat cgccgcaagc tcttcctccg ccggccagag gatgtttatc ggaaggtttt
    30301 tccacacgtc ctcgtttgca agacgctcga gccgcaaaag ctgctccgcg tcaatctcgc
    30361 ggtcaaagtc gatcgtcgcg ccgtgctcgt ccatgtggaa gccgatgttc tcgcagcccc
    30421 agagcgtgtg cgcatggccg gacaccagat gctcggccga gtggatctgc atgcgcgcaa
    30481 agcgcgtttc cccgtcaagc tcgcccgtca cttccgcgcc ggggacgagc ggagcgccgc
    30541 agaaatggac gatctcgccc ttttcctcgc gcgtatccgt cacacgcacg ccgccgagcg
    30601 caccggtatc gccgccctgt ccgccgccct cagggaagaa ggcggtgcgg tcgagcgtta
    30661 cggcaaagcg gccgccgcgc tcttcgcacg agaggacgcg cgcggcgaat tttcgcatat
    30721 gcgcgtcggc gtaaaacagc ttttcggtca tgggatcaga acttgaagag attcagcccg
    30781 gtcacatcgt ccttgaagcg atcgaccgca ccgtcgatca cgcggatgac catctcgcac
    30841 gtcgcgctca cgcgcgcggc gttgagatgc ccgccgacga ccgcgccgga ctcgttcgcg
    30901 gcgctcatgt gcagatggct gtagtattcg ccgttcatcg tgttcaccgt gccggtcagc
    30961 gacacgatct cgtgcgcgcc ggtgaacgtg agcgcgtggt actggcgctc gtccacgttg
    31021 tacacgccga cggtgaactc gtccgtcgcg ccgagcgcct caacgctcgc caaacggata
    31081 ttttcctttt cgcacagcgc gcgaagctgc gccgtgatct cctcgccgcg gttgaagcgc
    31141 gcaacgatcg tatttccaaa acgccggtag tccatagttt ttatctcctt tgcatgggat
    31201 gtatctgtat tatagccgct cggcgagcgt aaatcaaggt tgtcaaggcc cggtcgatcg
    31261 cgtataatat ccggagaaac gaaaggatcg gcctatggaa aacttttccc atcctctggt
    31321 ggactggtac aaaaaagagg cgcgccctct cccctggcgg gaggagccga cgccgtaccg
    31381 cgtgtgggtc tcggagatca tgctgcagca gacgcgcatc gaggccgcgc gcgaatactt
    31441 caaccgcttc acgcgcgcgt tcccgaccgt ggaagcgctc gccgcggcgg agcttgacga
    31501 tgtgctcaag ctttgggaag ggctggggta ctattcccgc gcgcggaatc tccacaaatg
    31561 cgccaaaacc gtgtgcgaga cttacggcgg cgtatttccc ccggacgccg cggcgctgcg
    31621 cgcgctgccc ggcatcgggg actataccgc cggggcgatc gcctcgatcg cctacggact
    31681 gccggaaccg gcggtggacg gaaacgtgct gcgcgtgctc gcgcgcatgg agtgcttcgg
    31741 cgagagcatc ggcagcgaca aagtgaaaaa gcgcttccgc gaagcgctcc gggatatcta
    31801 tccaacgggc gaatgcggcg cttttacgag cgcgctcatg gagctaggcg agacggtgtg
    31861 caccgtcgga acgccaagct gcgcgcgctg cccggtcacg gcgtactgcg cggcgcaccg
    31921 ctgcggcgag gaagcgctct accccgtgct gccggagaaa aaaccgcgcc ggattgaaaa
    31981 ccggcgggtg ctgcttttgc tctgcggcga tgcgtgcgcc gtgcagcggc gtgcggatac
    32041 ggggcttctc gcctcaatgt gggagctgcc gaacgacctt ttgccggacg cgccggaaac
    32101 cggcgagccc tgcggcgagg cggtgcacat tttcagccat gtagaatggc atatgcgcgg
    32161 gtacatcgtc cgctgcaggg agaagcttcc gggctatgtc tgggtaacgc gagccgagcg
    32221 cgaagcgctc gccatgccca cggcgttccg gtattaccga aaaaaactgg aggagatggg
    32281 atattgaata tctgtgtatt cggcgcggcg cgcgatgaga tcggcgagga ctttatggct
    32341 ctcggcacgg cgctcggccg cgagctcgcg gcgcgcgggc atacgctcgt gttcggcgca
    32401 ggcggccacg gcatgatggg tgccgcggcg cgcggggcct acgaaatggg cgggaagatc
    32461 atcggcgtcg tgccgtactt tttcaacact cccggcatcc tctttgagca ctgcacggag
    32521 cttatccgca tggaaacgat ggcggagcgc aaaacgatca tggaagagcg ctcggacgct
    32581 ttcatcgccc tgcccggcgg catcggcacg atggaggaaa tttttgaggt catcacgctc
    32641 tcatcccttg ggcggatgca gaaaaagagc gcgtttctcg atgcggaaag ctattgggaa
    32701 cccgcggtcg agctgctgcg cggcgcggtg gaaaagggct ttgcgggcaa ggagctgaat
    32761 gagcactatg cgcgcttcac ggatgtggaa gcgtgcctcg attatctgga aaaataatac
    32821 agcgggcgga gcagagcccc gccctcgcag aaattttcaa agaacgcccg taggttgggg
    32881 ctctgcccca accgcataca gttctccctc tttcacggcg ctgccgcgga tggagggaga
    32941 ttttttatgc cagcctctgc tgcatatacg caagcagtgt ctgcgccacg acggaatttg
    33001 tcgtgcgcgc gaacatacaa gtatccccca tttgccaagc aaatggggga tactaatctt
    33061 tatctgtgaa ctgccgtgcg gcgggatttg gcgaaaagcg tcgtcagcac cgcgagccag
    33121 aggaacgcca gagccgctgc tgcggcggtg atataggaaa cggttctcac cgttttcccc
    33181 tcctgcgcgc tgaacaccgc gcccgcctcc acgctctcgc cgttcgtgag cgtcacggtg
    33241 agcatgccgt tttcccccac ctgcacggag gaaatgccga cgccgggtgc gccgtcctct
    33301 cccgtgattt tgccaagatt cgtttccgtt ccgtccgtga gggcgacgat cagctccccg
    33361 gagccgttca gcgatacctc ccggatgccg acgccgtctt ttccgtcttt tccgtctttc
    33421 ccgtctttgc cgtccgtgcc gtcttttccg tttgctccgt ccttcccatc gacaccgtct
    33481 ttaccgtcag cgccgtctct gccgtccgca gcggcagctt ttacgccggt gtcggtatcg
    33541 ccgatccacc agtttccatt ctcgccgatg acgggcgtca gaccgtccgc gccattctta
    33601 ccgtcgacgc cgtcccgtcc gtccgtccct tcggctttca cgccggtgtc cataatgccg
    33661 atccaccagt tgccgttcgg accgacggag ggcgtccggc cgtcccgccc gtcctttccg
    33721 tcctctccgg tggctttcac accgagagat ttccacgtag tcccgctgtc gtaggatacc
    33781 tcccactcgt tcgtagcagg attgacgcgc agcttcgagc ggaatgggtc gtccatctgg
    33841 acaaggctca gcggcattcc gcccggatag ggacacgtct caaagataga cggcatataa
    33901 tccctgctga gatctgtttt ccagcggtag aactccgcgc cgatatacag ccccatctgg
    33961 ccgccaaaat gctgattggc agtaaagctc ccgccgttaa aataaagccc ttttccctcc
    34021 gcagaggtgc ttgacggccg gagatcgtcg gtgatgaacg ttccgctttc catcgtcaat
    34081 ctggtgatgt tcgctgttcc gccaaccaac gtgagtgaac cgctgcctcg aatggtaatg
    34141 ttctgagcga tgatcggata gtatgaggtg ttcacgctga agcggttttc tcccgtcaga
    34201 tctataacaa gcgggataaa gaccgagagt aaagcggagc ccatcggctg atttcccgtt
    34261 cccttaagat cggcgtttcg caaaaacagc gtcgtgccgt caaaatagaa attgtaatca
    34321 tccgccgttg cttcggaaaa gccgcggtta tccgacggat cgtttttata ataggtacca
    34381 acttcgagga acacgccgtt gatcttcgga tcgttccagt tcgcccgaac actcggaacg
    34441 ttcgccgctc ccagcggctg agcggtcatc tcttcgaccg acgctccaac cgtctcttcc
    34501 acaggctcca tgaccggcgc gggcgttgtt tccgccggcg cgggtgttgc ttccacgggt
    34561 acgggtgttt ccgcttccgt atcgtcggcc aacgccggaa cacagaggct cgtcagcgca
    34621 gcgcagacgc agatcatggc ggcaagaata cgatgcttca tggttttgtt cctccctgtt
    34681 tctgtctctc gggcgggtct ccctgtattt actttgtatt atgcaggaac agcgcacgct
    34741 ttgcaagaca ttttggtctg cgtgccagaa caggtaaaaa atcggctgtt tttcttcgaa
    34801 acagccgatt ttcgcgctta ttcgatttcc gccggaggca gcaggcgcgt catgctccgg
    34861 agactcacgg tgagcgtata cgcattgtgc agcagcgccg aggttgccgg gggcaggatg
    34921 cccgccacgc cgagacagat cagcataaag ttgaagccga tgatgtttcg gtaattcgtg
    34981 tggatccggt ccatgagcgc gcagctgagc cgccgcagtg tcaaaagcga atggaggtcg
    35041 ttcgcgccga tggtgatgtc cgccacctcg cgggcgatgg ccgcgccgtc gcggatggcg
    35101 atgccggcgt ccgcctcgga gagcgccggg gagtcgttca cgccgtcgcc gagcatgatt
    35161 acgcgccgtc cggcctcgtg ctcgcggcgg atgaagttgg ccttatcctc tggaaggacc
    35221 tcggagtagt actcgtccac gccgaccgcc gccgctacgg acttcgccgt gcggtcgctg
    35281 tcgccggtca tcataacaag cttcgtaaag ccgagagagc gcagcccccg gacgacgtcg
    35341 gcagcctccg cgcgcagcgg gtcctcaatg cagatgaccg ccgcgagcac accgcccgcg
    35401 gcgagataga gatgcgaata ctctgccggg agcgattcaa acttttcctc ttcccccgcg
    35461 gggatgacgc acttctcgtc ctcaaacacg aagtggtggc tgccgatgat gaccttttcg
    35521 ccgtccacgc tgcttgcgat gccgtgcgcg acgatatagc tcacgctcga atggcgttcc
    35581 tcatgggaga tatggcggtt ttccgcctcg gccacgacgg ccttggccat ggagtgcggg
    35641 taatgctcct cgagacacgc cgcaagagcc agcatgtcgt tttcttcctt gtcgccaaac
    35701 ggcacgaccg ccgccacgcg cggagcggca tgggtcagcg tgccggtctt atcaaagacg
    35761 atggtgtccg cctgcgccac cgcctccatg aagcggcctc ctttgacgga gatgtgatgg
    35821 atgccggctt ccttcatgcc ggagagcacc gcgatcggca tggagagctt gagcgcgcac
    35881 gagaaatcga ccatcagcac ggcgagcgcg cggttgacgt tgcgcgtcag cagccagata
    35941 agccccgtcg cgccgagact gtacggcacg agcctgtccg ccagatgcga ggcctgcgcc
    36001 tcggtgtcgg acttgagctt ttcggattcc tcgatcatat ggacgatgcg gtcgtagcgt
    36061 cccgagccca tgctcttatc gacgcggatc gtgcattcgc cctcttccac gaccgtcccg
    36121 gcatagacgt agctgcccgc cgtgcggcgc acggccagcc cctcgccggt gatgga
//
LOCUS       DAWAAE010000003        34186 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_540_length_34186_cov_3.921538, whole genome shotgun sequence.
ACCESSION   DAWAAE010000003 DAWAAE010000000
VERSION     DAWAAE010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 34186)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34186)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34186
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_34186_cov_3.921538"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2924..2933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8154..8163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8461..8470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8711..8720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6329 a        10711 c        10540 g         6566 t
ORIGIN      
        1 aggaaaaacc gaatttgcct gaacgccgtg agccgaaaaa tccctgactc acttttcaag
       61 attgagcgat gttcagctcc gcaggagcgc agccgatttc tctgaaaacg ttcaggggtt
      121 tggggcaaat gccccttcct ttttatgtag aaaagcccct tccgccgcgg cggaaggggt
      181 ttcgtttgac gaagctctcc cccgccggaa ccatcccgcg ggggagaaag ctttatttca
      241 aatgcttctc catatagaag agcccgtcgt ccttccggcg gacgcggtag ccgtccatat
      301 aggtgtagcc catcttttca taaaagccaa gcgccgccgt ggtggggatg agccaggcgc
      361 ggctgcactg cgtatagagc tcgtcggctt ccagtgcatg aaaaagcatg gtgccgtatc
      421 cctttccgat ggcctccggc gtgaggaaca gtccgtaaat caggcactcg tcctcaccgg
      481 tcttgcggac gccgcccatg ccgagaagcg cttcgccgtc gcagagcaca taggtgtgtc
      541 cggtctcgga aaagccaagg atcgcctcgg ggcggtattt ctccacgagc agggggatcc
      601 atggctcctc ctccggcgcg gtgtgtttgc tgttatattc cagcagcgtc cgtgccgcca
      661 gtcggctgag cgcagcttcc tctccgggaa caaaccggcg tatctccata accccgtcct
      721 cctgataatg atctgccaac gccatattac ccgtctccgc ccgatcccgc aagtcccctt
      781 aggctgcacc ccggctcccc gtcaggaacg ggagagccgg tgggtcatgc ggttccctct
      841 cggcggcata tccgccgctg caataaaaaa ctacaggcag ccgagcaggc gccgccgttt
      901 ttccctcccg attttgcaaa ccggagatgc agcaaaggcc ccctcggcga gggggccttt
      961 ttgtcagagt ttttcgacag cgtcggccat gccgtcgagc cagtcgccgg agaagacttc
     1021 catgagaccg ccgtcggagc aggcggcgag acgcggatcg atcgggagct tggcaacgtt
     1081 gggaatgccg tgcagcgccg cgagaccgtc ggcacggctc gcgccgaaga tctcatgctt
     1141 cgcgccgcag tccgggcaga cgtaatacga catattctcc acgagaccga cgacattttt
     1201 cttcatcatc gcggccatgt tgaccgcctt ttccacgatc atgcacacca agctctgcgg
     1261 gcttgtcacg atcaccacgc cgtcgaccgg cagcgactgg aacaccgtca gcggcacgtc
     1321 gcccgttccg ggaggcatat cgacgaacat atagtccaca tcgccccagt gcacatccgt
     1381 ccagaactgg ttgacgacgc ccgcgatcac cgggccgcgc cagagcacag gctccgtttc
     1441 cttttcgagc aggaggttga tgctcatcac cttgatgccg gtgtgggatt cgagcggctc
     1501 aatgccgttt tcgctctgga agcagttgcc gtggatgttg aacgccttcg gaatggaggg
     1561 gccggtgatg tcggcgtcga gaatggcggt cttatagccg cggcgctgca tgagcaccgc
     1621 gagcatcgac gtgaccatcg acttgccgac gccgcccttg ccgctcacca cggcgatgac
     1681 cttgccgacg cgggaggcgc tgttcagcgg ctgaagaagg ctctgcggtt cggtgcgctc
     1741 gccgcaggac gagccgcagg acgaacaatc gtgcgtgcat tcactcatag ggtaaaaatc
     1801 tccttggaaa tgataatgtg tggtttacag ccccagcagg gcggaggcaa tggaaaaata
     1861 gatcagcagg gacagaacgt ccacgatcgt cgtgatgaac gggcttgcca tgaccgcggg
     1921 gtcaaacccc agcttttccg cgaggatcgg cagcgagcag ccgacgagct ttgcgatgaa
     1981 cacgacgcac acaagcgtca gacacacgac cgccgcgacg agcgcggtga cgccgttgtg
     2041 gagcagcagg ttgtccacga gcatcatttt ggcgaaattc gcgcccgcga gacacacgcc
     2101 gcagaggacg ctcacgcgga tctccttcca gatgaccgcc cagagatcgg aaaactcgat
     2161 ctcgccgagc gcaagaccgc ggatgacggc gacggacgcc tgcgtgccgc tgttgccgcc
     2221 tgtgccggag agcatcggga tgaacgacga gagcacaagg caagccgcga gctggttttg
     2281 gaaatggttg atgatgagcc ctgtgaacgt ggccgagagc atgaggatga gaagccacgg
     2341 gatacgcgct ttccatgttt cgagcacgct cgtatagaga tacggccgct ccgagggcgc
     2401 cataccggcc atcttttcaa tgtcctcggt ggcctcttct tccataacgt cgatggcgtc
     2461 gtctacggtg acgatgccga cgagccggtt ttcggtgtcc acgaccggga gggcgttgaa
     2521 gttgtatttg gagaacatct gcgcgacggt ctcctggtcc tcggacgtcg tgacggagat
     2581 gacgttcggc tccatgatgt cctcgatcac gtcgtcatcg tccgagagga tgagcgtgcg
     2641 cagcgagagc gcgccgatga gccggccctt gtcgatcaca tagcaggtgt tgatcgtttc
     2701 cttatcgacg ccggtgcgct tgatgcgctt ggtcgcctcc tccacggtca tctgcgggcg
     2761 gagggagacg aactccgttg tcatgatgct gccggcgctg tcctccggat agcgcaggat
     2821 ctcgttgatc atccggcgca tgtcggggtc ggcctgccgg agaatgcgct ttacgacgtt
     2881 cgcgggcatc tcctcgacga gatcgaccgc gtcgtccacg tacnnnnnnn nnncggatga
     2941 gcagctcctg ctgctcgggc tgcatttcga cgaatgtttc cgccgccagc tccttcggca
     3001 gcagccggaa aaggagcggc agggcaagct catccgtctc ttcaaaaacc gccgccacgt
     3061 ccgccgggtt catcgttacg agaatgtccc ggagcgtggc gtatttcttc ccctcgatca
     3121 gcgcggagag cgtgctttcg agagtgacgg tagtctctgc catcgggtct tacctccttg
     3181 aatatttatg gggagctaag acacaggagg gcggattatg cgccggcggc cacagatgcc
     3241 ggcggtgttt ccatcgacaa tcgaccctcg cctgtgccgc tggtactgtg accggattcc
     3301 atgaccttca cctcacataa aacaaaataa aacttgccgg agcttttaac ttatatatta
     3361 tatgcccaaa tgcgccgcaa gtcaaaccga aacgggcgtt aatttccgct tttgccgtcg
     3421 ttttccggcg gttcggcgcg ctcgatcttt ttgatctgct tttcatattt ggtccgcgcg
     3481 cccatcacct cgcgcccgca gcccgtgcag cggagcttga agtccgcgcc gatgcgcagc
     3541 acctcccacc ggcacgcgcc gcaggggtga ttttttttca gtacaagaat gtctccgaca
     3601 cggatatcca tacatatttt cctccaaact gggaaatatc cccatagggg gtgatccaat
     3661 gcatacacag cagctcacgg cgcggtttcc gaccggggat gagatgaacg ccgcgcttgc
     3721 cgaccttcgc cgcagcgggg ccgtgtgcca cactggggcg atcccgtacg acgggatagg
     3781 gccgtcgccg gtgctccgtt tctcggtgcg cgaagcggac ctttgtctgg cgaaggccat
     3841 catacggcat gccggcggac gagtttagtc aaggcaaaag gcggagggcg cgccgtaaac
     3901 ccctcagtca gcctaacggc tgcctgctcc cctgttaggg gagccaagtt cgttgtcggg
     3961 aaaccggctt gcctccccta ataggggagg tggcaaaacc ggaggttttg acggaagggt
     4021 tttgcggcaa ggcaattccc cagccccctt tgcacaaggg ggcctttttt atttcttgat
     4081 cagctcccaa taagcctttg ccgcctgctc ggtcttgttg tcgccgtatt cgtagtaatg
     4141 cgcgtttttg agcgagtcgg gcagatactg ctgccgcacc cagtggccgg gataattatg
     4201 cgggtatttg tacccctgct cgcgctcaaa gcccgtgccg tcggcgtgga cattctgcat
     4261 ctcgcgcggg atgttgccgc tcttcccggc gcgcacgtcg gcgcgggccg cgtcgatcgc
     4321 ggcgacgacg ctgttcgatt tcggcgccgt ggcgagcaga atgcacgcct gcgcgagcgg
     4381 gagctgcgcc tccggcaagc cgagctgcag ggcgttatcc acgcacgcct tcacgatgct
     4441 caccgcctgc gggtacgcca tgccgatgtc ctcgctcgcc gagcagagga tgcggcggca
     4501 cgcgccgggc agatccccgg cctcgagcag ccgcgcaaga tagtgcagcg ccgcgtccgg
     4561 gtcggagccg cgcagcgatt tcatcagcgc ggagacaacg tcgtagtgct cgtcgccctc
     4621 gcggtcgtag cgcatggcgc tccgctgcgt cacggcgcgc gcgtcgtccg gcgagagcac
     4681 gaccttcccc tcaatgcgcg gcgcggcggt gaacagaagc tccacggcgt tgagcgcctt
     4741 gcgcacgtcg ccgccgcagc cctgcgccac gagcgtcacg acctcgtccg ggcagtcggc
     4801 ctcgatgctc tcccgctcgg agagcagccg cacggcccgg cgtaccgcgc cctccacgtc
     4861 gcgcgccgag atcgccttga actcaaacac cgtgctgcgc gagaggacgg cgttgtatac
     4921 atagaagtac gggttctccg ttgtggacgc aatgagcgtg atcgagccgt tctcgataaa
     4981 ctccagcagg ctctgctgct gctttttatt gaagtactgg atctcgtcga gatacagaag
     5041 aatgccgttc ggcgcgagca tggtgtccag ctcgtcgatg atcgcgcgga tatccgcaag
     5101 ccccgcggtc gtggcgttga gctttcgcag cgtgcggttc gtcttttccg cgatgatgcg
     5161 cgcgacggtc gttttgcccg tgccggacgg gccgtaaaag atcatgttcg ggatatcgcc
     5221 gctctcgata atgcggcgca gcagcccgcc cttgccgaga atgtggttct ggccgacgac
     5281 gtcgtccagc tccgtcgggc gcagctcgtc cgccagcggg cggtattttt tctccggcaa
     5341 tcggaacgcc tcctttgaaa tatgcgatat tttattatac cgtttttcct ccgtcaaaac
     5401 aaccgcgaat cttgtcacgg ggcgcagaaa cgtggtagaa tcgcggcgag gtgattctca
     5461 tgcggacaaa agaacagatc gacggaattc tcgccgcgct cgagcgcgaa tacccgatgg
     5521 cagactgcac gctcaccgag cgggacgcct ggcggctgct cgtacaggtg cggctcgccg
     5581 cgcagtgcac cgacgcgcgc gtgaatctcg tcacgcccgc gctcttcgtg cgcttcccga
     5641 ccgtggaggc catggccgcg gcggacatcg gcgaggtcga agagctcatc cgctcctgcg
     5701 gcttttacca cggcaaggcg cgcgatctcg tctggcaggc gcagcgcatc acggaggtct
     5761 acggcggcaa ggtgccggac aatatggacg atctcctgac gctccccggc gtggggcgca
     5821 agagcgccaa tctcattctc ggcgatgtgt tccgtcagcc cggcgcggtc gtggtggaca
     5881 cgcactgcat ccggctctcg aaccgtctgg gtctcgtcga taacgacaag gaccccgtgc
     5941 gcatcgaaaa ggcgctgcgc gccgtcctcc cgccggagaa atcgaacgat ttctgccacc
     6001 ggctcgtgct gcacggacgt gccgtgtgca ccgcgcgtaa gccgctctgt gacatttgtt
     6061 gctgtgcggc gtactgtaaa acgttaaatc aataaaattt tctattgcat ttcccgcata
     6121 cacggcgtat aatcctcata ttattttttg agataccgcg ggaaaaagag gggaaatgca
     6181 atatgacgat ttatgaccac tggctgtacc ggtttctgac ggggccgctg aacaacatgg
     6241 catggctgta catcctgctg ccgtgcgtgt tcgccggggg tatttatctg accgttggca
     6301 gcggagcggt gcagttccgg cgcttcggtt acgcgatgaa aaacaccgcc ggaaagctct
     6361 tcaaaaagca ggagacggcg cacggagcca tcacgccgct gcaggccgta acgacggcgc
     6421 ttgccgcgac cgtcggcacg ggcaacatcg tcggcacgtc gcaggcgatc gccatgggcg
     6481 gctacggcgc ggtgttctgg ctgtggatgg cggcgctgct cggcatggtc atcaagtatt
     6541 cggaagtcct tctggccgtc aaataccgcg agcgcgacgc gcgcggcgac tgggtcggcg
     6601 gcccgatgta ctatatcaaa aacggcatgg gcgataagtg gaagtggctc gccggggcgt
     6661 tcagcgtgct ggcggcgctc gcggcgttcg gcatcggcaa catgtcgcag gccaactcca
     6721 tcgtcggctc ggtgacggac gcggtgtgtg ccgtaaaccc cgatttcggc ggccaggcgg
     6781 cgctgcgctg ggggctcggc atcggacttg cgctgctcac ggcgctcgtg ctcttcggcg
     6841 gcatcaagcg catcggcgct gttacggaaa aactcattcc gtttatgagc gtggcgtaca
     6901 tccttatgac gctcatcgtc attttcggca acctcggcgg cgtcggccgg gcgttcaagg
     6961 agatcttctc cgcggcgttc gcgccccgcg ccattctcgg cggcgcggcg ggcatcacga
     7021 tccgtcaggc ggtcgtctgg ggattgcgcc ggagcgcctt ctccaacgag gccggtctcg
     7081 gctccgcgcc gatcgcgcac gccgccgcgc actgcgacgg cccggtcgag cagggcattt
     7141 acggcatttt cgaggtgttc atggacacca tcgttatctg cacgctcacc gcgctcacga
     7201 tcatcatcag cggcgtggac gtgacgttcg gcgtaaagcc cggcagcgcg ctcatcacgg
     7261 cggcgttcgg cacggtgttc aacggcacgt tctcctcggt gttcatcgcc gtggcgctca
     7321 cgctctttgc gtattcaacg atcctcggct ggtcgcttta cggctcgcgc tgcgtgcagt
     7381 acctgctggg tctccgcgcc gcgaaggtct atcagttcgt tttcattatc atgatcgtcg
     7441 tcggcagcgt ctcctcgctc gactttgtct ggaacatcgc cgacacgctc aacggtctca
     7501 tggcgatccc gaacttcatc gcgctcttcg cgctcagccc cgtgatattc aagatcacca
     7561 gggaatattt ccgcggcgtc gatctcgcga gcgccaaaca cgaataaaaa agcaacatcc
     7621 cgcctccatc ggagacggga tgttttgtat acgaagggga aaacccttcc gtcaaaaact
     7681 tcgtttttgc tacctcccct gttaggggag gcaagatagt tttctgcaaa ccggcttggc
     7741 tcccctaata ggggagctgt cgcggcgcag ccgtgactga ggggttgcct cccttgtgta
     7801 aagggaggtg ggttttgccg caggcaaaag ccggagggat tgtcaatttc cgaagactac
     7861 aattacaatc cctcagtcag cttcgctgac agcccccttt acacaagggg gccttaaaag
     7921 cgttttagga ttttgcttcg ccgtcggtct tgggtctcgg ctttctgcgg cggtggttat
     7981 tgcggcgggg cttatccgtg cgctcggcct tgtccccggc gggggcggcg ctcaccggcg
     8041 gcttttcacc gccgcggcgc gcgctgcggt tttccccggc gggctttttc tcgctgcgct
     8101 ccggcttctt ttccgcgcgc tcggggcgct cggctctttc ggtgcgctcg gacnnnnnnn
     8161 nnngcattcg cgccgccgcg cttccggcgg cgggagcggc tgcttttctt ctcgccggag
     8221 ggcttctccg cgctctgcgg cgcggcggac gcggtgcaca cgtcctccac gatcatcgac
     8281 ggcatgatcc agccgttcgt ctccggctcg gcgttctctt ccttgacctt tttgggcttg
     8341 ttgcggagga ggtcgggcag gggctggccc tccgccgggc ggccgccggg gatctcggcg
     8401 atctcgtcgg catcgtaggt gcggatctgc gcttcgcccg ggccgtccag ctttaccttg
     8461 nnnnnnnnnn tggcgcagga gattgacgag cgtgacgctg ccgtagccgt cctcggtctg
     8521 gacgaacgcg ccgtttttcg gcacgtgctt caaaagatcc tcatacgcct cctgctcata
     8581 gcgcaggcag cacatcagcc gcccgcacgt gccggagatc ttcaccggat tgagcgacat
     8641 cgactgcgtt ttcgccatct tggtgctgac gggctggaag tcgtcgagga actggtggca
     8701 gcagaagggc nnnnnnnnnn gctcgatgcg cgtgcggaac acgcccgcaa ggtcctttac
     8761 gagctcgcgg aagtccacgc gcccgtcggc ggtgaagaag aagagaattt tattcccctc
     8821 aaacgagcac tcgacgtcca cgagcttcat ttcgagcttg tgcttttcga tgagctcccg
     8881 gcagatgccg agcgcttcct tttcgcgctt gcggttgagc tccgcgatgc gcagatcgtc
     8941 cggcgtggcg cggcgcacga cgggacgcag cggctgcacg cagtcgccgt ccccgaccgt
     9001 gtgatttccc ttgacgcact cgccaagctc catgcccttg gacgtttcca cgacgagcgt
     9061 gtcgcccgtc ctcgcggtca cgccggcggg gtcgaaatag tacgtcttgc cgcggccgcg
     9121 gaattttatg ctgattactt ctgtcacggg attttcctca tttcctgatg tgtgagcgcc
     9181 ggaggtcatt tgccgtccgg cagataggtg gatagataac cggtcacgtg cttcgggccg
     9241 acattcagcc ggagatattc ctccgcgcgg cggagaaacg cttcgagcgc aagaagctcc
     9301 cgccggtcgg aaacgagctt cacggcgagc tcctccgccg cggtaacgac ctcgagaagc
     9361 tgcgcgttat cgagcttttc catcgccgtg cagcagcgca gaagctccgc gcggtcgagc
     9421 cgcgcttcga ggaagccccg ggcgcgctca ttttccggcg cggcagtccc gcccgcgagc
     9481 cggatctcgc cgcagcgcga ctgcaccgtt tccagcagct tttcgcggct ttcggcgcag
     9541 agaaggaaca tgaccccctt cggcggctcc tcgagcagct ttaaaaaggc gttctgcgcg
     9601 gcgatgttca tcgtgtccgc ttcgggaaag atgtatacct tgccgtcggc ctcgttcggc
     9661 agcacggcgg cgtccacggc gagcgcgcgg atctgatcga cggtgatctc gcgccgcagc
     9721 ttgccggact tgtccgccgc gcgctcgacg agcgccacgt ccggatggat gccggggaac
     9781 gcgccctgga acaccttgcg gcagtggcgg caggcgcggc agggcttcgc gccgctgctc
     9841 tcacagagcg ccgccgccgc catttcccgc gcgcgagagc gcgccgcgtc tccgtcgccg
     9901 gagattatat acgaatgcat catcttttcc atatcgattc agtataccat acacattttt
     9961 agtttgacaa gcgtttgcga attgaatact atggaaaaaa catcgtgagg ggggagaata
    10021 tgcgagccgc tatctgcgac gaccgcgccg aggacgcgca gctcgtcgcg cagtatgtcc
    10081 gcgaatgggg cgagagccgg gaagagagcg tcgcgctcga gatgttcccc tccgccgagg
    10141 cgttcctctt cgcctacgat gaggacaaga attttgacct tctgctgctc gatgtcgaga
    10201 tgggcggcat ggacggcgtg acgatggcca agcgcgtccg cgcggagaac accgcggtgc
    10261 agatcgtttt catcaccggc tacacggact acatcgccga ggggtacgac gtggcggcgc
    10321 tgcactatct cgtaaagccc gtccgcgagg aaaagctctt cgaggtgctc gaccgcgccg
    10381 cggcgaagct gcgcagcgac agccgcgcgc tcacgctcga gtgcggcggc gagatcttcc
    10441 gcgtgccgct ttatgagatc cgctatctcg atgtggagaa aaactacacg accgtccacg
    10501 cgaagcgcga ctacaccgta aagaaaacgc tcggcgcgct ctcggacgag ctggacgagc
    10561 ggttcttccg catcgggcgc gcggcgatcg tgaatctttc gtacatccgc cgcgtgacga
    10621 aaacggacgc gatcttatcc gacggcgcgg ccgtcccgct gccgcgcggc gcttacgaag
    10681 cgctcaaccg cgccattatc gcgtatcatt gagaggtgag agcatgagcc tattttcgcg
    10741 ccgcgtcgat aagcagctcg aagcctacca gcgcgagctg gtggacacgc attataaaga
    10801 ggtggagaac atgtaccgcc agatccgcgg ctggcggcac gactaccgga atcatatcca
    10861 ggtgctcaag gcgttcgccg ccaagggcga tatggacgcc atccgcgcct atctcgacga
    10921 gctggacacc gatctgaaca ccgtggacac cgtcgtcaaa accggcaacg ccatggcgga
    10981 cgccattttg aacagcaaaa tatccctcgc caaatcgcgc ggcatcacgg tgcaggccga
    11041 cgcgcacatc cccgtaaagc tcaaaatgtc ggagctcgat ctctgctgca ttctcggcaa
    11101 tctctttgat aatgccattg aagcgagcat ggctctgccg gaaaaggacc ggctcatccg
    11161 cgtgtacatg gacatgaagg gcacgcagct ttatatctcc ttcacaaact tcaccgccgg
    11221 aaagaagctc gaaaagaccg gcgggcgctt ccgcaccacg aagggcgagg ggcacggctt
    11281 cggtctcgtg cgcatcgacg atattgtcgc gcggctggac gggtatctct cccgcaacag
    11341 cgaggacggc gcattcacga ccgagatact gctcccgcag gtgtgagcga aacggttcat
    11401 ccccgaaaat aagccattcg tccccggtct gcgccgggga cgaagttttg tgatagtgtt
    11461 atcctcgcaa agaagaggtg gtttcgatga aagaaaaaac gaaaaagccg cgctaccgtc
    11521 tggggcagaa catgctctgg atgctgcggc aggcgcacgc ggcgcaccgg gacgatgtgc
    11581 cggtattcgc tgtcataaaa gcgctcgcca tggcgggcac gggcatttcg ggactcctgc
    11641 tcgcgccgga gatcgtccgc tgcgtagagg acggggcggg cttttcgcgt attctcgcga
    11701 cgatcgccgt gctcgccggg gtgctgctcg tgtgttcggc ggtcagcgcc tatctggagc
    11761 acgcgccgat gttcgcgcga gtcgatgtgc gccttgcgct catccgcaag atccattata
    11821 agacctgcgt gatgtcctat ccgctctccg aggacccgga agttctcaag ctgcaggagc
    11881 aggccgtacg cgcgaccata aataaccgct gcgcgagcga ggcgctctgg gtggatgagc
    11941 agaagtttct caccgcggcg ctgagctttg tagtgtatct gctgctgctc acgaatacga
    12001 gcgcatggct tctcgccgcg ctcaccgtga cgaccgcggc ggagtacttc gtcaaccggc
    12061 gcatcaacga gtggggctac cgtcaccgcg acgaggccgc cgcgctcgaa aagaaaatgg
    12121 actatgtttc cgacaaggcg cggagcaccg ttctcggcaa ggacatccgc attttcggca
    12181 tgcgcccgtg gctcgaagcc gtatacgata aaacgctccg cgcgatcgac gccttcgtcg
    12241 agcggcgcga gcgggtgtat ttctggacga acgtgatcga cgccgtgctc accgccgtgc
    12301 gaaacggcct tgcctattac ttcctgctgc gccagacgct cgccggggga atgggcgcgg
    12361 gcgactttct gctgtgcttc tccgccgtcg gcgcgtatac cgagcagctc aacggcattc
    12421 tcaccgagct cggcacgctg cggcgacaga gcctcgagct ctgtaccctg cgtgaatttc
    12481 tcgagctgcc ggagcccttc cgcatggagg gcggcaagtc cctgccggag tgtgcggatg
    12541 ggaaatacga gctgcggctg gaaaacgtct ccttccgcta tcccggcgcg gaggcggaca
    12601 cgctgcatgg catcgatctc acggtgcatc ccggcgagcg gctcgccgtt gtgggcttaa
    12661 acggcgccgg caaaacgacg ctcgtaaagc tgctctgcgg cttttatgac ccttccgagg
    12721 ggcgcgtgct cttaaacggc gaggacatcc gaaagttcaa ccggcaggag tactacacgc
    12781 tcttcactgc cgtgttccag aagttctcgg tgctcgaagc gacgctggag gaaaacgttg
    12841 cgcagacgcg cgagggcatc gacgaggcgc gcgtgcgcga atgtctcgaa aaggcggggc
    12901 tcacggagcg cgtggcggcg ctgccggacg atctcaaaac gcacatcggc cgagaggtgt
    12961 tcgaggacgg cgtgctgctc tccggcggcg aaatgcagcg gctcatgctc gcccgagcgc
    13021 tctataagaa cgcgcccatc ctgctgctcg acgagccgac cgccgcgctc gaccccatcg
    13081 ccgagaacga tatctatcag aaatacgccg ccatgaccgt ggggcggacg agcgtgttca
    13141 tctcgcaccg ccttgcctcg acgcgcttct gcgaccgcat tctgctcatc gacggcggcg
    13201 tcatcgccga gcagggcagc cacgaggagc tgctcgcgcg cggcggaaaa tacgccgggc
    13261 tctttgaagt gcagagcaag tattaccggg aggagggcga aaacgatgga agagaataag
    13321 aaaaaagtat cctcacgcga ggcgtggcgc ctgcaccgcc gcgcggcgaa gctcttttat
    13381 gccgaacggc ccgcgctgca cgcggtcaac tggctttacg ccgcgttcaa atcgctctcg
    13441 ccgtatgtgc tgatctacct ttccgcgcag atcatcggcg agctgacggg cacgcgggac
    13501 gcgcaccggc tcacggtgct cgccgttgcc gccgtggccg cggcgtcgct gctgccgctc
    13561 atcaccgccg cgctcgagcg ccggcagacg gttttgtacg acaacagctt ctatatcaac
    13621 cagaagatct acgccaacaa gctgctggac atggacttcc cccggctgga cgatcccaaa
    13681 acgcacgacg atctctccca gatcctccag aacgaaaaat ggtccggcta cggtctgggg
    13741 cgcgtgctcg atgtggagac gaagctgccg atgtccgttt ttggtgttct gggcgttgtc
    13801 gccctcaccg tgacgctctt cacgcagcgc gtgccggaaa gcgccggggc gtggacggtg
    13861 ctgaaccatc cgctgtttct gctcggctgc ctgctgctgc ttgctctgct ctcggtgctg
    13921 ccgtcgctgt gcatgaaccg cgcctcgatg ctcacggcag agctcgcgcc gctcgcgcgg
    13981 ctcggcaacc ggtttttcaa cttctttgcg gtcatggcga tcagcgaccg cgaccgcgcg
    14041 agcgacatcc gcatgtataa ccagcaggac atctgcgaga gctatgcttc gtccgatacg
    14101 ccgttcagta cgaacggccc gttcgcaaag ctcgcgtggg gccgcggcgg tgtgctgatg
    14161 atcgtgagcg ctttgttctc ggtgctgttc accgtggcag cgtacctttt cgtatgcctg
    14221 aaggcgctcg cgggcgcgtt cggcgcgggc agcgttgtgc agtatgtctc cgcgctcacc
    14281 gccctttccg gccatctctc gcagctcgtc gtatacggcg gcgacgtgcg cagcaacgcg
    14341 ccgtttctgc aggagctctt cgccttcctc gatacgccga acgagatgta tcagggctcg
    14401 ctcacgaccg agaagcgctc cgaccgcaag tatgagatcg agttccggga cgtgtccttc
    14461 cgctaccccg gcacggagaa ttgggcgctg cgccatctgt cggtgaagtt tgagatcgga
    14521 aaaaagctcg ccgtcgtcgg gccgaacggc agcggcaaaa caacgttcat caagctgctt
    14581 tgccgcctgt acgaccctac cgagggcgag atattgctca atggcataga tattcggaaa
    14641 tataattaca gagagtatat ggacgtattc tccgtcgtgt tccaggactt tcagctgctg
    14701 gcgttctcgc tcgggcagaa cgtggccgcc gcgccggaat atgaccgcgc gagagccgag
    14761 aaagcgcttg cgcaggccgg cttcggtgac cggctcaaag cgctgccgct cggaacggag
    14821 acgagcctct tccgcgattt tgacgagaac ggcgtcgagg tctccggcgg cgaggggcag
    14881 aagatcgcca tcgcccgcgc gctctataag gacgcgccct tcctcattct cgacgagccg
    14941 accgccgcgc tcgacccgat cgccgaggcg gagatctacg aaaagctcaa cgacatggtc
    15001 ggcgacaaga ccgccattta catttcgcac cggctctcct cgtgcaagtt ctgcgacgag
    15061 atcgtcgtgt ttgacgccgg aaagatcatc cagcagggcg cgcacgagac gctgctcgcg
    15121 gacgagagcg gcgaatatgc gcagctctgg aacgcacagg cgcagtatta ccgctgagtc
    15181 tccgtcgcac acacggcgaa gagccgccgg atgcggcgct cgatgagcga aaagacctcg
    15241 cagagtttgc gcccgcaggc gcaaataaag tgcaaagcat ttttgccggc ggcgtgtacg
    15301 tcggcaaaaa tacttttttg acaagctcaa acggaggggg agacccctcc gtttccgttt
    15361 cttgcggcgg aagggttttc cccgtcaagg tcttttcaac ggcggaaaaa tgcggtatac
    15421 tttttctgta tctcttagaa caaggagcga tgccgttatg atggataaat cgagagcgtt
    15481 tctccgcact ctgggtctgc cggggggcga tctgcacgct cttccgacct cggaggcaac
    15541 atttcccggc ggagcgcagt tcggcgtgga gatccccacg gtgaacacct ttgccgcggc
    15601 aaaggcgctg ctgcgcgaaa cgcagcgtct cggtgtcacg gtcaaccgca tcgacgaaac
    15661 gctcggggcg ttccgccaca cgcgcgcgga gcttctcgaa tatgccgcgc tctgccgcga
    15721 cagcggcgct gcgctcaccg tgtcgatcgg cccgcgggcc gcgtatgaca cctccgccac
    15781 acgcctttcg cggcagggcg ccgtcatcgg ctaccggctg cgcggcgagg agcagctcgt
    15841 ccgcgcgctt gaggacgcga agcgcgtgtg cgatctcggc atccgggggc tgctcgtata
    15901 cgacgagggg ctgctctggg tgctcagcga ggcgcggaaa accggcgagc tgcccgcgga
    15961 cacggtgctc aaggcgagcg cccactgcgg ccacggcaac ggcgcatcgt tccggctgct
    16021 cgagcagtgc ggcgcggaca gcattaaccc cgtgcgcgat ctttcgctcg acatgctctg
    16081 cgcgctgcgc gcgagcgttt ccgttccgct cgatgtccac accgactgtc cggagggctc
    16141 cggcgggttc atccgcacct acgaagcgcc cgagatcgtg cgctgctgcg cgccggtgta
    16201 tctcaagatc ggcaacagcg ctctcgccgc ccacggctca ctgccgacgg aggcggacgc
    16261 cgcgcgcatg gcgcagcagg ccgccatcgt gatggaaatg ctggagcggt atctgcccga
    16321 agcgcgccag ctcgctcgcg gcgaggggcg cggtctcgtc gccgaagcgg aggtccgcgt
    16381 atgagcgagc gcatcaagac cgccgtgctg cgcgattttt ccgtttcgct cagccgcgcg
    16441 gcggggcttt cggcggacga tgccgcgctt ttgaccgaca gtattctctt tgccgaatgc
    16501 cggggcgtca cgtcgcacgg gctgctgcgt ctgccgcagt acattgcccg gctccgctcg
    16561 ggcgcgacgg caagcaatgt tccgatgccg ttcacgatgg aatccggcgg cacggcgcgg
    16621 ctcgacgcgc agaacggtct cggccagctc gccgggcgga aggcctccga caaggcggaa
    16681 gagctcgccg cagaatacgg cgtttcgttc gtagcggtgg caaactccaa ccatttcggc
    16741 acgggcgcat ggtatgccat ccccgcggcg cggcgcggct ttttcgcttt caacatttcc
    16801 gcggcggcca gcgccatggc gccgcacggc gcggcgcagc cgcttctcgg caccgacccg
    16861 gtagggctgg cgttgccgcg cggcgagggg aaggctcccc tcgcgctcga tatggcgctc
    16921 tccaccgcag cgcgcggcaa gatccgctat gccgcgctga acggcacgcc gatccccgag
    16981 acctgggggc tggacgcgca cggcgcgccc acgaccgacg ccgccgccgt gctcgccggc
    17041 ggcacgctcc tccccgccgg cggcgcaaag gggtcggggc ttgcgatctt cattgagctt
    17101 ctctgcgccg tactcacggg cagcggcgct ctcggcggca cggggcttct ctccgatctc
    17161 tcgcgcccgg cgggaacggg gcacatcttc ggctgcgtgg atataaagcg ctttctgccg
    17221 ctcgaaacgt ttgaagctct gtgcgaggag agcctttcgc gcgtcacttc tctcaccccg
    17281 gcgcagccgg gcggcgagat tttgctcccc ggcgagccgg aggagcgccg tgccgccgag
    17341 agcgcggcaa acggcgtgcc ggtagctccg gcgctgcgcg atacgctcaa cgcgctcgcc
    17401 gaagagctcg gctgcgcggc aagaatttaa aaaaacctta tgcattttgg aaggaggccg
    17461 tatcccccat gaaactacgc atcgacacct cccgctccta cgctctcgcg ctcgaaggcg
    17521 gcggagcgaa gggcgcgtac cagatcggcg catggaaggc gctgcgtgag gcaggcgtga
    17581 agatcgacgc cgtcgcgggc acctccgtcg gcgcgctcaa cggctcgctc atcgtgatgg
    17641 gcgatctgga aaaggcggaa aacatctggg aaaacatccg ctattcgcag gtcatggacg
    17701 tggacgacga gacgatgtcg cgccttttga agggcgacgt gaagctcgac gagctcgaca
    17761 gcgtggcgca gcagatgttc gaagtgataa aaaaccgcgg ctttgacgtt acgccgctgc
    17821 gcaagtggat atcgcaggtc gtggacgaga aaacggtgcg caaatccccc gtggagcttt
    17881 tcatcgacac gttctccctt tcggacggga agctgctcga gctgcgcgcg aaggacctgc
    17941 cggagggcac gctctgcgac atgctgctcg caagcgccta tctcccggtg ttccgcagtg
    18001 aaaagctcgg cggcaagcgc tacgccgacg gcggtctgcg cgatgtgctg ccgctgcatg
    18061 tgctcattga gcacggctac aaggacattc tcgcgctgcg gctgttcggc atcggcgtgg
    18121 agcgctcggt gaagatcccg gaggatacgc gcgtctatac cgtcgagccg acggccgatc
    18181 tcggcagcac gctcgaattt gaaccggcgc agagccggga aaacctgcgc gccggatatt
    18241 acgatatgca gcgcttcctc tacgggctgc gcggcacgaa atactatgtg gactgcgatt
    18301 gggacgagac gaaggcgctc tcgcttttgt cggacgccgc ctccgccggg gagagtgtca
    18361 gcctgcgcac gctctgcgaa aagcagctcc ctgcgctcgc caagcggctc gacgcggaaa
    18421 agggcgatta cattgatctc gccgtggcgc tcgtcgaggc cgcggcggag cacaagggcg
    18481 ttgatccctg gcagatcgtc acggcggacg cgctgctcga gcttgccggc ggcggagccg
    18541 ctctcgccgc ggcatttgcc gaaccggcgg cgcgcaagct cgcccgtcag ggcggaacgg
    18601 cgcggccggc cccggcgctg ctggggcgca agcggcccgc gcagggctgc cgccgtccgg
    18661 acggcggcga ggggctgaac gtctgtctga tgaacgactc gttcccgccg gtcgtggacg
    18721 gtgtggcgaa cgccgtggtg aactatgcga agatccttac cgagaccgag ggactctgcg
    18781 ccgtggcaac gccggagtat ccgggcgttg tggacgatta cccgttcccc gtcgtgcgct
    18841 atccgagcat cgacacgacg aagatgaccg gctaccgcgc cggaaacccg ttcgccccgg
    18901 cagccgtagc ggagcttgcg gcgatggact ttgacatcat ccacacgcat tgcccgatcg
    18961 tttccacgct actcgcgcgc agtctgcgcg aggcgatcga taagccggtc gtgttcacct
    19021 atcacaccaa attcgatatc gacatcgcca aggccgtccg cggcaaaacg ctgcaggacg
    19081 cggcggtgaa gctcctggta agcaacattt ccgcgtgtga cgaggtatgg gtcgtgagcc
    19141 gcggcgcggg agaaaatctc cgctctctcg gctacgcggg cgactacatc gtcatgccga
    19201 acggcgtcga tctcccgcgc ggcaaggcat ccccggaggc cgtggacgcg ctgagcagaa
    19261 aatgggcgct gcccgccgat aagccggtat acctgttcat cgggcgcatc atgtggtaca
    19321 aggggctgcg catcattctc gacgggctca aaaaggtgcg cgacgccggc ggcgatttct
    19381 gcatggtgtt cgtcggcgac gggcaggacc gccccgccgt ggaggaatac gccgcgcagc
    19441 tcggtctcac cgacctctgc cgcttcaccg gcaccgagcg cgaccgcgac acgatccgcg
    19501 cgtggtatac ccgcgccgat ctgctgctct tcccctcgac gttcgacacg aacggcctcg
    19561 tcgtgcgcga ggcggcggcg tgctcgctcg gcagcgtgct cattgaaggc agctgcgccg
    19621 ccgagggcat caccgacggc gacaccggca tcctcattcc cgaaaacggc gacggtctcg
    19681 ccgcggcgct gctgcgcgag ggcgcggacc gcgcgtattt cgcccgcatc ggcgagacgg
    19741 cctccgagaa gatctatctc tcgtgggaag acagcgtgaa gaacgcccgc gcgcagtacc
    19801 gcagcgtgat cgagcgttgg aatagcggcg agctgccgcg ccgccgcgtt ctccccggcg
    19861 cgccggagtg gaccggcgac gtcgccgagc tcatcaaccg cgcccgcacc ggctgggaca
    19921 aggcaagaga agcgctggac cggtatctgt aagatttcgg taaaggcccc ctcgcgcatc
    19981 tctccatcag aagcggcaag tacggtttgc agtaagctgg cttggctccc tccttgaggg
    20041 agctggcagc tatgctgact gagggagtta ctccttccgt ctcggcactt tgtgccgagc
    20101 cacctccctc aaagagggag gctttctttc ccgcaaaaaa tcccctgccg cgcggttttc
    20161 gcgcggcagg ggattttctt taataattca ctttttcccg aagaactctt caaagtagtc
    20221 ccgcgtggcg tcgcggccgg gggcgtcctt tgtttccttc gcgtccggca ccgggcgggt
    20281 cggttcgtcc tccggcgctg cggcgggccg ctcgccggtg acgcgcgtcg gctcgtccac
    20341 ggcggagtcg ggcgtgagca tcacgtcctc gcggcggcgg ctgcgcggcg cgccggtcgt
    20401 ggcgcggacg cggcgctcat gccgcagctc ttcctcgtcg tcctccatat cgagccggat
    20461 ggtgcgcgcg agctccagcc gcttctggta cgcgcggcgc tttgagcggt agcgcaccac
    20521 aacgaaaata tacgcggcaa agagcagcac cagcgcccag aacgcgagaa tgacgcccgg
    20581 cgtgcgcagc gtttcgcgga gctgatcctt catgtactcc acgcgcgaga gatcgaccga
    20641 ggtggtcgtg acgagcggcg aggtgccgag caccacgccg tcacggatca cgctgatctc
    20701 gccgacgacc tgtcccgcgc tgaccggcgc ggtcagcccg tccgcggcga cgtccggcga
    20761 gagggtgatc gtccgctcga ccgccgtcag atcgacatcg ttcggcagga aggcgcggat
    20821 ggtcgtgctc gggcgggccg taacggtgtc ggcgtccgcg cccattgcaa cgggaacctc
    20881 cgtcacggtc tcggtggatt tgagaacgtc ctgatagctg aagttgttga agccccattc
    20941 aaagagcgtg cgcgtgtcgg caaagttgcc gtagtaccag ctgccgtcgt cctgctgata
    21001 cgcctccgat ttaagcacga cggaaaggag cttcacgccg tccttttccg cgctggaagc
    21061 gagacagtat ccggcgtcgt tcgtgtggcc ggtcttgatg cccttggcgt attcatagcc
    21121 ccagtcgccc ggatagagag ggttcgtggg gttgacgagc gagttcgtgc tctccagaac
    21181 gcgctcggag gaaacgttcg tgtccggcac ggtgtagttg atggtgctgg cgatcgtcat
    21241 aaaaagctcg tgcttggcag cctcgcgcac gatgcgcgaa aagtcccagg cggtcgtata
    21301 gtggttcgcg tcgggcaggc cgtgggtgtt cgtgaagttc gtgccggtgc agccgagctc
    21361 cgcggcgcgg gcgttcatgc gcgcgacgaa atcggagatc gagccgctga cgtattgcgc
    21421 gatcacgttg caggcgtcgt tggccgaggc gaccatcgcg cagtagagaa ggctttcgag
    21481 cgtcatcgtc tcctgctgca tgatgtacac gctgctgccg ccgacgatca tatcaaaatc
    21541 aaatcccggc tgcgccgtga ccgggtcgga gagagcgatc ttgccggact cgatatcctc
    21601 cacggcgaga aggacggtca tcaccttcgt gagcgaggcg ggatagagcc gctcgtcggc
    21661 atttttcgta tagaggaccg tttcctggtc accgtcctcg gcgaggagca cggcggcgta
    21721 cgtcgtgtcg atccccggat cgtcgatcgc catggccgcg ggcgggacga gccccagcgc
    21781 gagcgcggcg ataagagcgc atattgcaaa gcggattttt ttcattcttg acttagcccc
    21841 cgtaagagta tggcataaca gaggtattat agcgtaaaac tactgaaaaa gcaacctttc
    21901 ccggagatcc ggtatctgga agcggaagaa aaaccgccgc gcggccgtct tgtgcgcaag
    21961 aaaagcttgc aatatggcgc tctatagtat aaaatatttc cgctaaatag tggatacaaa
    22021 taatttattc acatacgagg aggagtttcc tatgatttcc gcaggcgatt ttcgcaacgg
    22081 agtaaccttt gaaatggacg gccaggtcat gcaggtcgtc gagttccagc acgtcaagcc
    22141 cggcaagggc gcggcgttcg tgcgcacgaa gatgaagaac gtcatcaccg gcgctgtcgt
    22201 cgagaccagc ttcaacccca ccgcgaagtt cgaggaagcg ttcgtcgagc gccgcaacat
    22261 ggagtacagc tacaacgacg gcaaccttta ctacttcatg gatcccgaaa cctacgagct
    22321 tgagcccatc gacgagagca tgctctccga cagcttccgc tttgtgaagg aaaacatgat
    22381 ctgcaccgtc agctcctaca agggcaaggt gttcgcggtc gatcccccga cgttcgtcga
    22441 gctgaccatc actgagaccg accccggctt cgccggcaac accgccacca acgccaccaa
    22501 gcccgccaag ctggagaccg gcgcggagat caaggtgccc ctgttcctcg aagagggcga
    22561 gcgcatcaag atcgacaccc gcaccggcga gtatctcgag cgcgccaagg gctgagaagt
    22621 cctgcaataa cgtccaacca tcaacaaagg gagggtttct tatgacaaca tccgaaaaag
    22681 tggcctatct gaagggtctt gccgaaggca tgggcattca ggaggacggc ggccagggaa
    22741 agctgttcca tgtcattatc gacattctgg aagatctttc tctcgatctc gcggacacga
    22801 aggacgcgct ggtcgatctg accgacggcg tggaggagat caacgaagat ctcgccgagc
    22861 tcgaaaacga gttttatgag agcccctgct cctgctgcgg cggagacgac gaggacgaag
    22921 agtacgacga cgaggacgaa gacgacaccg tcttctactc cgtggagtgc ccgggctgcg
    22981 gctttaagct gaccgtggac gaggacacgc tcaacgcggg ctccttcgag tgcccggagt
    23041 gcggcgaagt catcgacttt gaaaacgccg ccgtcgagga agtcgttttc gacgatgaag
    23101 acgacgagga cgaaaaagac tgattccaac tgcatacaaa cagccggagg gaaattccct
    23161 ccggctgttt ctttttgcag cgcggggcgg tcccgcgttt ttcgtcaggc cagaaaaccg
    23221 ttcagctccc gtgcggcatt tcgcagatgc tccacgatcc tctccttctg cgggaggatg
    23281 cgttccgccg ggccggagat actgatggca tactctgcgc ttttccccgg ctgcgcaaag
    23341 atcggcacgc cgagcgcggc aacgccgggc tcatacatct ggtcgttgag catataccct
    23401 tcccggcgaa ccgcggcgat cttctccatg agctgcgacg aggaaagaat gaacggacag
    23461 ctccgctttt caaatacgac cgcatcgcag taatcccgta tatcctcgtc cgtctgcgtg
    23521 gagaggatgg caagaccggt gcagcacaaa tgcaaaagca gcagtgagcg cgttgggctg
    23581 ctcgcggcga tcagtccctg ctgcggcagt atctcatcga gcagcgttgc acggcaggcg
    23641 tcgtaccgcc gggtaaagtg gaccgtttcg ttaatctccc gggagagcgc cgtcagcaca
    23701 ggcttggcca gctcgaccgc agcgcgagac atctgcgctc tcgcgccgag cgccgcgagc
    23761 tgtatgccgg gacggtagcg tccttctcgg tccgcattca gatagccgcg cttttccagc
    23821 gtataaacta tgcggtaggc aaccgtttta ttcagcccgc tccgctgcgc gatctgcgag
    23881 agagtgagct tatcctcttg taaaaaaaga cttaagactc caagcgcatt gtccagcgac
    23941 tgaagcagat attgatcctt attgacgggc tccattccat tcctccgtga ccggccggat
    24001 aatttcccgc ggggtgtccg gcaaaaagca atcatagcat agcatatttt tttttcaagt
    24061 gcaattttag gggttgattt ttccgaaagt gcgtggtagc ataaagctgt aatttcggta
    24121 tgcgaattct ttgtttcgcc aaaagaaact ataaggaggt gcaatatgaa aaaacttgtt
    24181 gtgatcaatc ttggatcgac ttcaacaaag atcgcgtatt ttgaagacgc gaaaatcgtt
    24241 ttctccgcct cgctgccgca tccggcggag gagatccagg cgtttgcgag caactgggat
    24301 cagtacgatt accgaaagag cgcgatcctc ggttttatgg agcagcacgg catagtgctt
    24361 gccgagctgg atgcctttgt cagccgcggc ggccacacgg aaccggtgca cagcggcgtt
    24421 taccgcatca cgccgaaaat gcttgagcag tcgcgcagca tgaaatacgg caaccacgtt
    24481 tccgatctcg gcgtccggct ggcgtatgac cttgctccga acaagcagaa cgcttttatc
    24541 gtagacagtc cctgcacgga tgagtatgac ccgctctcgc gctactccgg tctgccggag
    24601 ctgcctcgag tgagccgctt tcacgtgctc aaccagaagg cgtcggcgcg gctctactgc
    24661 cgggagcacg gtctcaacta tgaggacgtc aatttggtcg ttgtccacat gggcggcggc
    24721 acgtccgttg cggctcacaa aaagggccgt cttgttgacg gcaacaacgg gcttgacggc
    24781 gacggcccgt tctctaccga ccgcaccggc ggtctccccg tcggtgggct gatcgacatg
    24841 tgctattccg gaaaatatac ccacgcggaa atgcgccgga aaatcaaggg gcagggcggt
    24901 cttatggcct atgtccacga cacggatgta aaaaccatcg aagccaaggc gcttgccgga
    24961 gacgaggact gcaaaaatgc gctcgacgcc atgatctacc agacagcgca ggagatcggc
    25021 aaaaaggccg ttaccctcaa gggcgacgtg aacgccatca tcctgaccgg cggcattgcc
    25081 cacagcaaat atgttacgga tgtgatccgg gactatgtat cgtcgatcgc cccggtatac
    25141 gtctatcccg gtgagcatga aatggaatcg ctcggtgaac agtcctacga agccctgatc
    25201 ggaaaagcgg aggtatttga actatgatgc tgaaaaactt tggccagatg gagcagtttg
    25261 tccgggagca gtccggaaaa aagcgcgtgg cactcgccgc ggcgcatgac gccgatgctc
    25321 tcggctcgct gatgatggcc gtggaccgcg gcattgccga ggccattctg atcggccgcc
    25381 ggaaggagat cgtccgcgtg cttgatgaaa tgggaaagga cgccggagac ttccgcatca
    25441 ttgaggaaga ggacgaccgc gccgccgcag acaaggccgt cgagctcgtt cactgcggcg
    25501 aagcggatat gcccatgaag gggctgatgc acacctcgac cttcatgaag gccattctgg
    25561 acaaggagaa gggcctgaag gaagagggca atctgctcag ccaggcgtcc gttttcgaga
    25621 tccccgagaa ggatcggttc ctcatcatct ccgactgcgc cgtaaacatt gatccggatc
    25681 tggaaacgaa gatcaagatc acggaaaacg ccgcagcgct cgcgcacacg ctcggtatcg
    25741 agcagccgaa gatcgcggtg ctctccgccg tggaggtcgt aaaccccaag atccgaagca
    25801 ccgtagacgc cgccgcgatg cgggagcgtc tctcggaccg gtatctcatt gacggtccgc
    25861 tcgctctgga caatgccatc gacgaggcgg cggcccacca caagggcatc gaaagccccg
    25921 tggcaggaaa tgccgacatt ctgatcgtgc ccgatctctg gagcgggaac atcttttcca
    25981 aaggtctcgt gttcttcgcc cacctgcgca gcgccggaac gctcaacggt ctgaaagcgc
    26041 cggtcgtgat gagcagccgc acagacaccg tggaaaacaa atatctctcc atccttaccg
    26101 ctgttttgca aaccatccgc tgaaaaaata aaacaacaaa cagaaagaag gagacccatt
    26161 atgagtgaga aagtaatcaa aaagcagcgc aatgtggcga tcgagctctg gcgcttcatc
    26221 atggccattg cgattatcgg cttccatgcc ggcttcatca ttgcgcgctc ctgcaacgag
    26281 accaccggtt tctttatgga agtgagcaac tggtttttcg gctcgagcga aacgctgctc
    26341 gtgttcacag tgaccgccgg ctatttcatg gccgcgcatt tcaccaagcg gaaaaacgat
    26401 ccggcgtata tgtcccgcag cgccgcgagc cgcgcctggg agtatactgc cgcccgcatc
    26461 aagaccctgc tccccgtact catcatcggc tacatcattg cgatcgtcgt ctgcaccagc
    26521 ttctactatc ccgactacaa aacgcaggac gtcctcgtta tgatcgtcaa cagcatctgg
    26581 gagtttctcg gcttccacgc cgccggtctg cgcagcacgg gcaacgagtt cttcaatctt
    26641 aacggcccgc tgtggttcat ttccggcatg atcatcgccg gatacttcat gtactgggcg
    26701 ctctgcaaga acgaggacgt gttctccggc ttcgtcacgc ctctcctcgc catcttcttt
    26761 gccggctggt ggtgctttac cggcacccgt gcttcgcaga cggcctggtc caccttcggc
    26821 gcgcagacca cttccaccaa cggcatgggc ggcagcgcca ccgacgcgac cgctaccatc
    26881 ggtttcaaca acggcatcat ctttatcgtg atcggcatgc tcatcggcgt gatggtcttc
    26941 tacatggtcc gcagcctgtc cgcgcgcgaa ttctccaagg gagccaaggc cgcgctcacg
    27001 gtgctcaacg ttgtcgtcag cgcgctgctt ctctggtaca ttctctggca gcccacttac
    27061 ttcaagctcg agcgctggac ggtcgcattt ctgtgcatcg ctttgatcac gctctccctg
    27121 ctcaaccgcg attatctcac cgctgcgctg aacaacaagg tcacgaacag catcttcggc
    27181 tatctcggca gcatctccct gtacatttac atgctgcact acccggtcgt catcgcaacg
    27241 ctccgcattc tcggccacaa cacgccggag acgatctact ccttctggac agtgtacatt
    27301 cccgccacgg tcgcaacggt cgtgctcagc gctctcgtca acgagatcat gaagcgcacc
    27361 attatgaaga aaaaataagc gatgcctgtc ggtgttctta ttgacgtagg ctccgttttc
    27421 ctgggcggtc tgctcggcgg acagctgggc agccgcctcc cggaaaagct caagaaggat
    27481 ctgacgctgg tgtttggcgt atcctccatg gctatgggcg tcacatatct cggcaaggtg
    27541 agcacgctgc cggcggtgat cctctcggtg atcctgggcc ttatgatcgg cgagaccgtc
    27601 catctcgacg gcgcgatccg tgccggagcg ggaaaaatga acggcgtcgt ttcgcggctt
    27661 ctgccgaaaa gcagcgccgc catggatgag acgcgcatgc atcagctcgt gagcattatc
    27721 gttctcatct gcgcgagcgg cacaggcatt ttcggtgcgc tcgattccgg gctgaccggc
    27781 gaccacaccg tgctctgcac aaaggccatt ctggactttt tcacggcggc gatcttcgct
    27841 tcctcgctgg gaagcgtcgt cgcattcgtg gctctgccgc agcttatcct tcagcttctt
    27901 ctctttttca gcgccgggct gatcctccct ctggcgagcg ccggtatgca ggcggacttc
    27961 gccgcctgcg gcggcgtgct gatgctggcg accggtctcc gcatcgccaa catcaagtcg
    28021 ttcccgatcg cggatatgct cccggccatg gtcctcgtca tgccggtgtc tgcactctgg
    28081 acgaagatcg ccggcttgat gctctgacat ctgccgtttg aaatcgccgg aaggaattcc
    28141 ctccggcggt tttcctttca aaaaggtgtc catggggtga tccccgtgga cacctttttc
    28201 tctcatgcgg caagatgccg gacgagcctg gcgagtgttt cggtgctggt gtaataatca
    28261 tagatcttct gcacgcgctg ccgcgcagcg tcgttccagc acacatcccc ccgctgcgcg
    28321 cagagcgttt ccgcgtgggt ctgcacgtcc gtatagaagt attccgaaag ctcatagcag
    28381 acgtaccgct cgtccgcgta cacgcgccgg tcttcgacct ccgaaagcgg cacgagctgg
    28441 ccgtggacga agagatggtc cgcgttttcg acgtcctcgc ggaaatacgt gaacaggtac
    28501 acgaccggga tgggcgcgcc gccgtccgta tccccctcgc gctgcgctac aagtgcggag
    28561 aggacgagcg cttcctcgcc cggcgcgccg ccgaccggca ggccgtaggc gtcgagcgtc
    28621 gtatagtttt cacctgtgag aagatgctgc gcgtccgcgg gagcggcggg atcgaagatg
    28681 acgccctcct ccgcatcgcc gatgacaaga tccgacgggg cggggcgcgc cgcgcgccct
    28741 cccagcggcg ctagcagctc ggcgcacacg gcgtagtact ccgcattgtg ggagcggcgc
    28801 tgtgcaacgt cgccggacat ttcaaagtat ggccgcagcg tttcctccac gcgggccgtg
    28861 agctccgcgt ccgtctccgc gggggcgagg ggcgcggcgc tgggctccgg cgttttctcc
    28921 ggtacggctt ccgccgtttc cccggtctct tccggcgcgg cggcatggat gaagcagatc
    28981 cagtcctgac caaacacaaa attgctgttc gtcaaaacga gatagtatcc gccctgcgcg
    29041 agcggttcgt ccagcgccgc aagatcgtaa gcgcgggaca gatcgagcgt catcacgccc
    29101 tcggtgtgcg gcgcgaacgc cgtgttcgca aaacgcacgg ccgcgccgcg cttcggcgcg
    29161 atctcctcgg acgtatccca gcgcagaagg cggagctgct tctggaattt cagctccttg
    29221 tccgagcggt tttccacatg caccgatacg acgccgtccc cctcgtacac cgcgccgagc
    29281 gcgagttcgt ccccggcaag cggcgagaag aggtcggcgg cgagcgccgt tacggtaagg
    29341 agcgccgcga gtaccgccgc ggcaagtgcg atcggcacag cgcgcgcggc gcggcgtttt
    29401 cggagcggca cgccggtttt ttcggtaaat aggctttgaa tgttacggta gttttcctcg
    29461 ctgaatgagc gcatattact ctccttccgc gccggtcacg ctcgcgcgca gccgccgctt
    29521 tgtctgatac agccggttgt ccacctgctt tttgtccagg ccgagcgccg cagcgatctc
    29581 gcgcggcttc tgcccgaaat agtagcgccg aagcagtatc tcgcggtccg gctctgcgag
    29641 cgcgtccacc gctgcgttga gcgcggcacg ccgttctccg gcgagcagcg cctccggcac
    29701 gcccagcggg tcggccagct cgatctcctc gagcgatacg gcgcggcagc gcaatatctc
    29761 ccggctgcgg tcgagagcgc gcgagcgcgc cacgaccgaa agccatgtct tgagcgagcc
    29821 gcggcgggga tcgaactttt ccggatgctc ccataaatat acgaacgcat ccgccgcggc
    29881 ctcttccgcg tcctgctcgg tgccgacacc gcggagcgcg gcggcggcca cggtatagag
    29941 atgccgggaa tagcgcgcga tcacgtcggc aacggcgcgc tcggagcgcg cggcaagcgc
    30001 cgcggcggtc ttttcgtctg tcaagggttt cacctcgttt tctttttgac cggtctatta
    30061 tacagtacgg caaaacgggc ggaaatactt aaaaagttgt cagccggaat cgtccaacga
    30121 caaaagcgtc agcgggttgc cccgctgacg ctttttccgc tttatgttgg cgctgcttcc
    30181 tccgtcggct ccggtgtggt ttccggcgtt ggctccggct cccggagcgg ctcgggcacg
    30241 accgtcacga gcgcggtgcg cgtgctttcc agaagctcct cccgcacggc ctccgcacag
    30301 gagccgtccg cgagatacgc cgccgcgttc caccgcgcgc ggagctgcgc gggatagccg
    30361 gacgcatccc cctgcgccca cgccgcggcg aagccctcgc agagcgccgc gccgagatcg
    30421 tcccgctccg ggaacgtcag ctcctccagc cgctgcgcgg cggagagcgt ctccaccgcc
    30481 ggatcggaaa atccgccccc ggccgccgag cgcagcgcgg cgagaaccgc atcgcggaac
    30541 acctccgcgt ccttttcctc cagtccgggc gcgaaaaacc agatggccgc gtccgcgccc
    30601 gccgacgccg tgccgcatac ggtctccgta tccggcagcg cagcgcacac gctcttatcg
    30661 agcagcgacc ccgtgcgctc cagcgccgcg gcgagcgcca cgagccggac gcgcgtccat
    30721 tcgcccgcgc gcgggcagac gatgccgaaa tatacgcccc cggctccctc cccggcaagc
    30781 gataccgttt ccttcaccgg cttttcgatg cggcagtagg ccgcgtcggg tgcgagcgct
    30841 tccccggcat cggcagcgga gaacaccccg tccagccacg agaggatatc ctccgccccg
    30901 tccgcatcgg cgcagacgac cgcgagcata ttctccgccg agaccggccc cgcgccgttc
    30961 ccggccgtgt cgctgccggg gagaagatat gtgagaaacg cgcggtacgc cgcctccgcg
    31021 tccggcacgg cttccgtctc cgcgccgaaa aatgcgccga ggctctcctt tgccccgcgc
    31081 agaagctccg cagccgagtc tgccgtgagc gtcacgcggc gcagcccgtc cgttgtgcgc
    31141 gccatgagcg tgagtgccgg ggacgcgccg gtatcttcta tgtacacgac cgccgcgccg
    31201 gtcacctcgt gcgtaagata tgcggtcttt ccgccgggcg tatccagccg ctcgatcttt
    31261 tcgacacgaa agccggagag ctgctcgccc gcgccgggca gcgcggcgct ccggcgcaca
    31321 gaaaagccgc tgcctaccgt gaacacgaac acgataagca gcagccatac gattttccga
    31381 gatagttttt tcattggtgc atgtcccctt ctccgcaata ttgttttaca gagaaagtat
    31441 atgcgcggga aattcaggat atgacgcccg tgccgtcaaa ggcgggaatt tcttcctcac
    31501 cggaggcgga gagctcctgc acatacccat ccgagaggcc ggtctcgtcc aacgccgcga
    31561 gcgcggcgtc ccattcctcc ggcgtgagcg tccggcgcag ctccgggaat tttttgagat
    31621 ccccgcaggg ggtatactgc gccatgagcg atacgagcac cgtgccgggc gcgaacgtgt
    31681 ccgcgatcca gaaaagcgcc gcgcgcgtat tttcgatctg ccccggcagc acgagatgcc
    31741 ggatcataac gccgcggcgg agaaggccgt cttcgtccag ctcgaacgcg cccgtgtgct
    31801 cgaccatacg ctttatggca ctttttgccg ccgccggata gtccggcgcg cgggaatagc
    31861 gcgcggcaag caccgggtcg gtatatttaa gatccggcag aaagacgctc acatgctccg
    31921 cgagcgcgtc cacggtatcg accgtttcat atcccgacgt attccacacg accgggatct
    31981 tcggctttgc gatggtgagc gcttcccgta tttccggcgt gaactgcgtg ccggtgacga
    32041 gattgagatt gtgcacgccc tttgcttcca gcccggcgaa gatcgccgcg aggcgctcga
    32101 cggagaccgt ttcgccgatc ctcccgcggc tgagcgcata gttttggcag tatacgcagc
    32161 cgagcggaca cccggcgaag aacaccgcgc cgctcccccg ctcgccgctg atgcagggtt
    32221 cctcgccgta gtgcggcgcg gcgcgcgcca cgcgcacggc gcttcccata tggcacacgc
    32281 ccgcgcttac cgcgcggtct gccccgcact gccgggggca gagacggcag tcaggcatgc
    32341 agcacctcgg cgataacggc gtcgtccgag cggctggtgc gctccggctt tctgcggccg
    32401 cactccatgc ccttgagcgc gagcttgagc atctttttga gcgtatactt gctctccccg
    32461 gcttcgcggg cgcggcggtc gtaggagacc gtgccgacct tcgcgccgag ccggttcaca
    32521 agcccgcgca ggaaaagatc gtccccgtgg tagtgcgaga gcgcttcgag cgcggcggtg
    32581 tccatgagac ggtaatcggc gtggtcgtaa atgagatcgg agccgaagag cttcatcaat
    32641 ccgtagtacc cgcgggcggt ggtgcgcttg aggaacgtat cggtcgcacg cgaggcgcgc
    32701 acgccgcaga cgatgccgca gccttcctta tacttctcca ccattttgtc catcgcgtcg
    32761 atgtcgtcct gcaaatcggc gtcgatgctg atggtgatgt cgcaccgggt gcgtgaccac
    32821 atgagccccg cggtgagggc gttctggtgg ccgcggttgc agatctggcg cagaccggtg
    32881 aaatgcgcgt cgctctcgtg gagcgagcgg atgatgctcc acgtggcgtc cttcgagccg
    32941 tcgtccacga ggacgatgcg gctttccggc gcgatggcgc ccgcgtcgat cagatcgccg
    33001 agctttttgc gaaagaccgg cgccgagagc ggcagagcct cttcctcgtt gtagcagggc
    33061 acgacaatat aaaggatcgg aagcgatttc atgaggtttt ctccttcccg aataatatcc
    33121 ggcaatgcgc caaatactga tactggcatt ttacaccaaa acaccgcgca gtgcaacgaa
    33181 acattttgtt tgaaagaaca tggttttttt cgtaagaaat gaacgaaaac caacatattt
    33241 cacttgcaaa atgcgcatcg gtgtactaca atgcgctcag aaggaaaaaa ctattattag
    33301 gaggtctagg atatgaatta caaaaagaag agcatccgcg acgtgagctt cgccggcaag
    33361 aaagtgctgc tgcgcgtgga cttcaatgtg cctctggaca agacgaccca cgagatcacc
    33421 aacgacaagc gcatccgcgc cgctctgccc acgatccagt acctgctgga cgacggcgcc
    33481 gcgctgatcg tctgctccca tctcggcaag cccaaaaagg accccgacgc caagaccaac
    33541 ctgacgctca agcgcgttgc cgtccgcttt gccgagctgc tcggaaagcc ggtcgagttc
    33601 gccgaggata ccatcggccc cgacgccaag gcgaaggccg ccgcgctcaa gccgggccag
    33661 gtcctgctgc ttgagaacac ccgctttgat ccccgcgagg agaagaacga tcccgagttc
    33721 gccaaggagc tcgcttccat ggcggatgtg tacgtttccg acgcgttcgg cgcggtgcac
    33781 cgcgctcacg cctccaccgc cggcgttgcg gactatctgc ccgcgtactg cggcttcctc
    33841 gtggagaagg agctgaccgc tctcggcggc gcgatggacg atcccaagcg cccgctcgtg
    33901 gccgttctcg gcggcgcgaa ggtcgccgac aagctggccg tgatcgagaa cctgctcaag
    33961 aaggccgaca cgctcgtcat cggcggcggc atggcgttca cgttcctcaa ggccaagggc
    34021 tacgagaccg gcgcctccct gctcgacgag gagaagatcg actactgcaa gaacatgctc
    34081 gccaaggcgg aagagtccgg cgtgaagctg ctgctgccgg tggacgtcgt gtgcgccgac
    34141 cgcttcgccg aagatgccga aaccgtcacg gtttcggtcg atgacg
//
LOCUS       DAWAAE010000004        30595 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_649_length_30595_cov_3.131991, whole genome shotgun sequence.
ACCESSION   DAWAAE010000004 DAWAAE010000000
VERSION     DAWAAE010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 30595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30595
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_30595_cov_3.131991"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4556..4572
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9046..9055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22476..22485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22742..22751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26969..26978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5566 a         9744 c         9430 g         5798 t
ORIGIN      
        1 cagtatgagg gcatttccgc ccggcagaac cgcgtgtgcc cttgtcaatc gacgcgaggc
       61 aatgaaaata taaaaaattt atttatcttt ggaggaaaca catcatggtc aaaatccgtc
      121 tgcgccgcgt cggcgccaag aaagccccgt tctaccgcgt cgtcgtggcc gacagccact
      181 tcgcccgcga cggccgcttc atcgaagaga tcggcaccta cgatcctctg accgagcctg
      241 ccaccatcaa gatcgacatg gagcgcgcca agtactggat ctccaacggc gcccagccca
      301 ccgacacggt ccgcggcctg ctgaagaaag ctgaagcctg attccgaggg ctgccggaat
      361 gaaagaactg ctgacctaca tcgtgcaaag tctcgtggag aaatcggacg aggtctccgt
      421 gacagagcac gagaccggcg gcgaaaccgt ttttgaagtg cgggtcgccc ccggcgacat
      481 gggcaaggtc atcggccgtc agggccgcgt ggtcaaggag atccgcgtgc tgatgaaggc
      541 cgtcgcccag cgacagggaa aaaaggtctc ggtcgagatc ctcgactgag tctcaaaccc
      601 aaaacccaaa ccgcaggggt ggggcgctgc cccgtccctg cggtttccat atctggaggt
      661 ttacctatgg caaaggagcc gtatctcgaa gcgggaaaga tcatcaacac gttcggcgtg
      721 tgcggcgagg tcaagctcga cccgtggtgc gacagcgccg agtttttaaa gccgctcaaa
      781 acgctctaca ttgacggcgc gccgcgcgcc gtcgcctcgt cgcgcgtcca caagggcatg
      841 ctcatcgtcc gctttgcgga tgtggaggac gtgaacggcg cgatgcttct caaaaacaaa
      901 atcgtgtact tcgcccgcag cgacgtgcgt ctgccgaagg gacggcactt tgtcgccgat
      961 cttctcggcg cggcggtcgt ggacgagcag ggcacggaga tcggcaagct caccgaggtg
     1021 ctcgatatgc ccgccgggca ggtctacgtc gtgcagggcg agaccgagca cacgatcccc
     1081 gccgtgccgg aatttattct tgacatcgac gcggacgaga aaacaatccg cgttcatctg
     1141 atagaaggga tgtgacgcca tgcgcatcga cattctgacg ctcttccccg acgcggtgga
     1201 ggcgtcgctc aagatcagca ttctcggccg cgccgagaag aagggcattc tggacatcca
     1261 cgccgtccag atccgcgact ataccgaaaa caaacagcag caggtggacg attaccccta
     1321 cggcggcggc tggggctgcg tgatgatggc gcagccgctc aagtcgtgcc ttgacgacgt
     1381 gtgcgccaag gcgggcgaaa agcgccgccg cgtcatttac atgtccccgc agggcaagac
     1441 gttcgaccag agctgcgtgc gccgtctcgc cgcggactac gaccatctcg tgctcgtgtg
     1501 cggccactac gagggcgtgg acgagcgctt catcgaagag tgcgtggacg aggagatctc
     1561 cctcggcgac tttgttctga ccggcggcga gatcgccgcc atggcggtga cggacgccgt
     1621 gtgccgcctt atccccggcg tgctcgcgga tgaggagtgc ttcaccggcg agagccactg
     1681 ggacggcctg ctcgaatacc cgcagtacac ccgcccggac gtgtgggagg ggcgcgaggt
     1741 gccgcccatt ctgcgcagcg gcgtagaccg cgagatcgcc cgctggcggc ggaaaaagat
     1801 gctcgagcgc acgatggaca agcgcccgga gctttttgcc aaattcgtgc cgcagacgga
     1861 cgaggacaag gccctgctcg acgagatcga cgccgagcgc cacccgcgcc ccgtgccgcc
     1921 gaagatcgac tgccgccgcg cggaggaagc cgatctgccc gccattctcg ccattgcgga
     1981 cgaggcaaag gcgtttctcg ctgcgcaggg gctggaccag tggcagcacg gctaccccgc
     2041 ggaggaggac ttccggcgag acatcgagcg cggcgacggc tggctcttca ccgcggacgg
     2101 aaatccggcc ggttatctct gcatcagccg ccggagcgag gcggcgtaca ccgccattga
     2161 gggcgaatgg ctctcgtgcg cggggccgta tctcacgatc caccgcatca tggtttcgga
     2221 cgcctaccgc ggaaaaggca tcgcttccga aatgatctcg ctcgcggacg actttgccca
     2281 cgcctactgc tgcgcgggtg tgcgcatcga caccgccccc ggcaatgagc ccatgcgcgc
     2341 catgctgcgc aaaaacggct ttgagacctg cggcacgatc cggctcgtcg gcggcgtgga
     2401 cgacggcgcg ctgcgcatcg cgctcgaaaa gctggtggaa cgatgaatct gaccgactac
     2461 cgcgacataa cggcgctttt gcagcgccac ggcttccgct tttccaaatc catggggcag
     2521 aactttctga ccgccgcgtg ggtgccgcag cagatcgccg cggaaagcgg catcacggca
     2581 gctgacggcg cgctcgagat cggtcccggc gtcggctgtc tcacggcgga gcttgcgaaa
     2641 accgcgggcc gcgtcacggc cattgagctg gacgagcggc tgcggggcgt gctcggcgaa
     2701 acgctcgcgg attttgataa tgtgtcggtc gtttttgccg acgcgctcaa ggcggatctg
     2761 ccgaccatct gcgccgaaac gctcggcgaa agaccgtgga aggtgtgcgc caatctgccg
     2821 tacaacgtga cgacgccgct catcaccgcg tttctcgagg caggctgctt tgagagcgtc
     2881 accgtgatga tccagaagga agtcgcgcag cggctgtgcg ccgcgcccgg cacgggcgag
     2941 tacggagcct tctccgtgct cgtgcagtgg tacgccgagc cgaagcttct ctttgacgtg
     3001 ccgccgcact gcttcgtgcc gcagccgaag gtgacgagcg ccgttgtgcg catggaccgg
     3061 cgcgccgcgc ctcccgcgag cgtggacgac gaaaagctct tcttccgcac cgtgcgcgcc
     3121 gcgttcgccc agcggcggaa aacgctttca aacgcactgc gttctgcctt ctccgagctg
     3181 gaccgcgccg ctattgactc cgccatggag gaaaccggcc ttccgcccgc cgtgcgcggc
     3241 gaaacgctct ccatcgcgca gtttgccgcg ctctcgaatg cgcttttacg caagttcgac
     3301 aaatgaaaat tgcaaatttc ctcacttttt tcgatatacg ccattgccaa acggaacttt
     3361 tccgtctatt atgaataata taatgtaaaa gcaatgggag gctccgggac atggcatata
     3421 cgatcaacca ttatatcacc gactgggtcg aggagagccg cgcgctgatg cagccggaca
     3481 aggtcctctg gatcgacggc acggagacgc agctcgagga gctgcgccgc gaggcgtata
     3541 tcaccggcga gctcatcaag ctcaaccagg aaaagctgcc gggctgcgta taccaccgca
     3601 gcgcgctcaa cgacgtcgcc cgcgtcgagg gccgcacgtt catctgctgc aaaaaggaag
     3661 aggacgccgg ccccacgaac aactggatgg cgccggacga gatgaaagca aagctgcacg
     3721 atatctatgc cgggtgcatg cgcggccgga caatgtacgt tattccctat tcgatgggcg
     3781 cgatcggctc gccgtttgcc aagtacggca ttgagatcac cgactcgatc tatgttgtct
     3841 gctgcatggc gatcatgacg cgcgtcggca cgaaggtcta tgaagcgctc ggcgattcgc
     3901 cggactttgt caagggtctc cactccaagg ccgagctcga cgccgacaag cgctatatcg
     3961 cgcacttccc ggaggaaaac ctcattatgt ccgtcaactc cggctacggc ggcaacgcgc
     4021 tgctcggcaa gaagtgcttc gcgctgcgca tcgcctccac gctgggccgt gaggagggct
     4081 ggctcgccga gcacatgctc attctcggcg tgcagaaccc caagggcgag atccgctacg
     4141 tctgcggcgc gttcccgtcc gcctgcggca agaccaacct cgccatgctc attcccccgg
     4201 cggcgtggcg tgagaagggc tggaagtgct ggtgcgtcgg cgacgatatc gcctggctgc
     4261 gccccggccc ggacggacgg ctctgggccg tcaacccgga aaacggcttc ttcggcgtcg
     4321 cccccggcac gagcgaaaag acgaacccca acgctctcgc cgcgacgcgc aagaacacca
     4381 tcttcacgaa cgtcgtcctc aagccggacg gcaccgtctg gtgggaaggg ctcgacaaga
     4441 acccgccgac ggacgcgctc aactggaagg gcgagccgtg ggatcccgcc tccggcgtgc
     4501 ccggcgcgca tccgaactcc cgcttcacct ctccggcgat caactgtccg tgcatnnnnn
     4561 nnnnnnnnnn nngctacaac atgggcgact actggcagca ctggctggat atggaaaagc
     4621 tcgtcaccaa gccgccggtc atcgcaaacg tcaactggtt ccgcaccgac gacgagggcc
     4681 atttcctctg gccgggcttc ggcgacaacc tgcgcgtgat cgagtgggtg ctgcgccgcg
     4741 ccttcggcga ggtggacgcg cgcgaaacca cgctcggcta tgagccgtac cccgaggata
     4801 tcgatcttga gggcagcggc gtgacgaagg aaacgctcgc cgatcttctc tccgtcgatc
     4861 ccgagctctg gaaggccgag tgcaagggca tccacgagtt ttacgccaag ttcggcgaca
     4921 agctcccgca gcgtctcgcc gacgagctct ccgctctcga agcgcggctc ggataaaaaa
     4981 tataccaaac cggggcatga ccgcatcacc gcgtgtcatg ccccggtttt tcattgctcc
     5041 gatgtaccgg agcttttttc tatttcatcg agcacccatt gcgccatctc acgcacttcc
     5101 ggcgattcat ccccgcacgc cttccgaatg accggcaggt atcggagatc gccgctgttc
     5161 cgcatggcgt tgagcgcgtt cgttttgtac cgccactcct tccccttggg gatgtaccac
     5221 agcttcggct gcacagcaga gcgcagccag tcgtaatcgg caagaacgat tttctccagc
     5281 gcagactgtc cggcccacgc ctccagcccc ggaaacttct ccgtatcctt ccacgcattc
     5341 gtattgtgcg ggcaggcatc ctgacagacg tcgcagccga atatccacgg tccgatctcg
     5401 ccgcgccgcg gctctgtccg catatcccag gcgtcccacg tcgtcaggca ggaaacgcag
     5461 gtattccggt tcatcgtata tggtgcttcg agcgagtgcg taggacaggc gcgtatacac
     5521 agatcgcagg actccgcgca gggccgaagc gtgttctccg ctgtgagctg cagcggctca
     5581 tcgataagga acgcttccag atactgatat gagccctttt ccgtatagaa gaaattgttt
     5641 ttgcgcacga tcccgatccc cgcctgcatt gccgcccagc gcagcgcggt aagcgcaaaa
     5701 tcgcggttga acgccacgcg cagcccgctg tcccgaagaa acttctccaa tgccgcgccg
     5761 gctttgtagc cctgcgagtc cgggtttcgt ttcccgtccg tcagatagta cttggcaata
     5821 tgcccgcgca cggcggcagg aatgcggtac ttcccatacc agaacgacac gatcacaatg
     5881 gcttttgccc acggaaattc cttttccgtg tatgcaaatt ttcagaagcc ttcgtacttc
     5941 cttctcgtct cgggaaaatg ctccatgcgt tcttcgagct tagcttcata cccgcgcatc
     6001 atctccacgg gaataatgcc gcatttatcg tagccgagct caatggcctt ttctgcgatc
     6061 tctgctgcac ttaccgccat aaggacattc tcctcctgcg tttcgattca ccgggctttt
     6121 tccgggtaat tatagcacac tttacggcac atcacaaaga gccaagagca aacagaacct
     6181 tttcataatc ctttacatag taccggatgt ttttacggcc tttggagagg atggcatgtc
     6241 cctccgcctc cagtttttcc ttctgtgact cgattcctcc cggatatttt tcgttgagct
     6301 ccccgttcgc cttcagcgtc ctccaatacg gcgtttcatc cccggagcgc tgatggctcg
     6361 cccacgccgc gatagagaca aagatccccg ccgtgatcgg atcggtaaaa tccgcaccgc
     6421 tttgcttcgc gaaatgcgct cgtatctccc ccacggtggt caccctgccg taggggactc
     6481 ttttcatcac gcggtcatag tcgagcggcg gagcaaagta cattttgttc ccgccatatt
     6541 tctcaatgct tttccggtcg gtaataattt gtatcttggg catgtccttg ctgtcatgca
     6601 gcatggcgtt gaaatccttc gtgctttcgt ttgccatttc gccacctccc gatgcaagga
     6661 tatcgtgttt cgaagcgcaa tgcaatgaga cggtttaaaa aatatttgcc ttcacagctt
     6721 ttaagtacac atcctccaaa gcgcagaaaa gctgctgccg caatcgcggc agcagctttt
     6781 tgctgtaaga aatcttattt cgcgtaatcg acggccttgg tctcgcgcag gacgtggacc
     6841 ttgatctggc cggggtacgt aagctcggct tcgatcttct tggcgatgtc gcgcgcgagc
     6901 aggacgatct ggtcctcgct gacctcttcg ggcttgacca tgacgcggat ctcgcggccg
     6961 gcctgaatgg catagcagct cgcaatgccg gggaacgagc gggagacttc ctccagcttt
     7021 tcgagacgct tgacatagtt ctcgatgttc tcgcggcgcg cgccgggacg ggcggcggag
     7081 atcgtgtcgg cggcctgcac gatgcaggcg atgaccgtgt gcggctccac gtcgccgtgg
     7141 tgcgcttcga tcgcgtggat gacctcttcg gactccttgt acttgcgggc aaggtctacg
     7201 ccgatggtca cgtggctgcc ctcgacctcg tggtcgatgg acttgccgag atcgtgcagg
     7261 agaccggcgc gcttggccag ggcaatgtcc acgccgagct cagcggcgag cagaccggag
     7321 atctgggcga cctcgatgga gtggttgagc acgttctggc cgtagctcgt gcggtagcgc
     7381 tggcggccga ggagcttgac gagatcgggg tgcaggccgt ggacgttcgt ctcaaacacg
     7441 gcgcgctcgc cctcttcctt gatggtctgg ttgacctcgc gctgggcctt ggcgaccatt
     7501 tcctcgatgc gggcggggtg gatgcggccg tcctgaataa gcttttcgag cgcaaggcgc
     7561 gcgacctcgc ggcggacggg atcaaagctt gagaccgtga tggcctccgg cgtatcgtcg
     7621 atgatgagat cgcagccggt gagcgtttcg atggaacgga tgttgcgtcc ctcgcggccg
     7681 atgatgcggc ccttcatttc gtcgttgggg agaggaacga cggaaacggt gatctcgctc
     7741 gtgtggtcgg cggcgcagcg ctggatggcc gtggcgatga cctcgcgggc cctctgctcg
     7801 gcctcgtcct tgaactgggc ttcaacttcc ttgaccttgg cggccatttc gtgtgtgcag
     7861 tcggcctcaa cgctcttgat gagatactgt ttggcttcct ccacggagta gccggagatc
     7921 ttttcgagca tgtcgagctg gctcttcttg atgagcttga tctcctcctg ctgggcatcg
     7981 gcttcctgga gcttgcggtt gagagtctcg gtcttctttt cgagcgcttc ggtcttgcgg
     8041 tcgagatttt cttccttctg ctggagacgg cgctcctgct tggaaacttc gttgcggcgc
     8101 tcttttactt cgcggtcgca ctcggtgcgg gtgcgatgga tctcttcctt cgcctcgagc
     8161 agcgcctcgc gctttttgtt ttcagcggct ttaatgcctt cgttgatgat gcgtttcgct
     8221 tcatcctcgg cgctagcgat ctcgcgctcg gaaacctttt tccggtacat gacgcccagc
     8281 acaaagccga tcagcaaaaa caccagcacg gcaatgatgc tggcgacgat catccatacg
     8341 gacatttgcg gcataagtcg gtctacctcc ttcttcctta gtcttcttat gataaagggt
     8401 cattttgaaa ttaaaggagt tccccatctc tcccataata tcataaggta cattttatcg
     8461 attctttaac gttatgtcaa gcggtttctg ctgtgcagga aacaaaatat tacaatttgt
     8521 aatatgcagg gcaaaactgc acgccgcagc ctttcccatg ggggaatttt tgtatgtcaa
     8581 gaccgaattt tgccaaaaat cccggcccga ttttctacgc tttttcgcat tgactttcca
     8641 taatttccgc cgccggagcc ggcggaacgt gcctcttccg ggaaaacgca accgggattt
     8701 caaagagtta tcaacatttc ccacagactt ttcaacatcg attttttctt tgtatttcaa
     8761 tgcttatccg gagaatatcc agaaaatgtc aatcacgcaa acggcatttc gcttgtttcc
     8821 ggctgccgtt atccggtttc cataacgaac cgtcccgcgc gatatgcagg gaaaaatgca
     8881 cagtactgca aaaccggcgg aggccgaagc ccccgccggt atcaaatctg aagtaaggtc
     8941 tgtgtgattt atcgcataaa tccgccctcg cgcatggaga cgtagtcgtt tccggcgacg
     9001 atgatgtggt cggaaagctc cacgccgagc agatcgagcg cgcggnnnnn nnnnnggcaa
     9061 gccgcgccgt ggaggtctcg tcctctacgg aaggcagcgc cacgccgctg cggtggttgt
     9121 gcgcgaggat gacggcgctc gcgcggtact gcagcgcaag ctcggcgagc ctgcgggcgt
     9181 tcacttcggc ggcatccacc gtgccgcggc ttatcgccgc gcagcggatg ggacgcttcc
     9241 gcgcgtcgag cagcagcgca tagacggtct cctccgtctc gtacatgaac cgcgggatga
     9301 aatagcgtcc cgcagcctcg gcggaatcga tcggctcgtt cgacggcgtc ttgtcgagca
     9361 ggtaccggcg ttctcgccga tgcccggcac cgcgagcagt tcctgctcgt ttgcctccag
     9421 cacggcggaa aacgagccga accgctcgag aagcgcgtga gccatgccgt tcgtatcgcg
     9481 ccggggcgcg gcataaaaga gcagcagctc caaggcgttt atatcgttga agttttcgag
     9541 accgtggccg cggaagcgtt ccttcatccg cgagcggtgg ccgtcgtgga cgcccatttc
     9601 cctcgcccct tattttttat cttatccgat ctgcttttcg agcggcatcg ttgcgtaggc
     9661 gttgagctcc ggcccggcgg tatgcccggc gaggaactca taatagccct ggcagccgat
     9721 catcgcggcg ttgtcgccgc agagacggag cggcgggacg aaaagctgcg cgcccgcctt
     9781 ttccgcggcg gcagcaaaat cggcgcggat gcgagaattg gccgcgacac cgcccgccac
     9841 ggcgatcttt ttgtacccga gctccgcagc cgccgccatc gtgcgcggca cgagcgattc
     9901 gctcaccgcg cggcagagcg acgccgcgag ccccgcgcgg tcgagcgcct cgccggtctg
     9961 ctcgcagtgg tgggcaagat tgataacggc ggtcttgaga ccggaaaagc tcatgtcata
    10021 gggattctcg ccgatgaacg ggtgcggcag cgtgtaggcg tcgtcgcgcc cctccttcgc
    10081 cagccggtcg atcggcgcgc cgccgggata cggcaggccg agcacacgcg cggatttatc
    10141 aaagcactcg cccgcggcgt cgtcgcgcgt ctggccgaga atgcgaaggt cggtatagtc
    10201 gcgcacgtcc acgagcatcg tgttgccgcc ggagatggca agacagagga acggcggctc
    10261 aagctccgga taggcaatgt agtttgcggc gatgtggccg cggatgtggt gcaccgggat
    10321 gagcgggatg ccgagcgccg ccgcggcgct tttggcaaaa ttcacgccca cgagcaccgc
    10381 gccgatgagt cccggcgcat acgacaccgc cacggcgtca agctcggact ttttcacgcc
    10441 cgccgccgcg atcgcgcggt cagccagccg cgagatgacc tccacatggc tgcgcgaggc
    10501 gatctccggc acgacgccgc cgtatacggc gtgcaggtcg gcctgcgaga agatctggtc
    10561 ggtgagcacc tttcgtccat tttcggtcac ggcgacggcg gtctcgtcgc acgtggattc
    10621 cacggataat atcagcattg cgttaccccg cctctttttg gaagaatttc gacatcagaa
    10681 tagcatcttc cgccggtttt tcatagtagt tttttcgttt tcccacgatc tcaaagccgt
    10741 ttttttcata gagccgccgc gcggcgagat tgctctcgcg cacctcgagc tgtaaaacgg
    10801 cggcgccgcg ccgctcgctt tcggcaaaaa gcgctttcaa aagcgccgcg ccgatcccgc
    10861 ggcggcgaga gtccggcgcc acggccaagt tgccgatatc gccctcgtcg agcacgaagc
    10921 acgcccagac gtagccgaga aggtcattcc cttcttcggc ggcgaggatg acgccgccgt
    10981 ccgtttccca aaggcgcgcg agcattcctt cgtccgcgcc gtccggaaat tcggcgcgct
    11041 cgagcgcggc aacggcgaga atgtcggaaa gctccgctct gcgaacgatc atgtcccgtg
    11101 cgcctccgcc cagtttctgc cccagccggc gtctgcggtg agcggcacgg tgtaagctac
    11161 ggcatgttcc atttcctcgc gcagcacctg cacggccttt tcccgctccg cttccggaca
    11221 ttcgacgatc agctcgtcgt gcacctgcaa tatgagcttt gccttcatgc cctcggcgcg
    11281 aagccgctga tccacatgca ccatggcgag ctttataacg tccgccgccg tgccctgaat
    11341 cggcatgtta agcgcgacgc gctcgccgaa cgcgcgcagc gccggaacgt tcagctccgg
    11401 cagatagcgc cgccggtgaa agagcgtttc ggcgtagccg agcgccttgg cgcgccggac
    11461 ggcttccttc tggtaatcgc gcacgccgcg gaagcgcgcg aaataggcgt ccatatactc
    11521 cttcgcctcg ccgtagctga cgccgatgtc ctgcgcgagc gagaaggcgg aaatgccgta
    11581 cacgatgccg aagttcacag ccttcgctct ccggcgctcc tcggcggtga cgtcctcaag
    11641 cggcacgccg aacacctgtg aggccgtgac gcggtggaca tcctcgccgg agagaaacgc
    11701 cgcacgcatc gcctcgtcgc ccgagatgtg tgcgagcagg cgcagctcga tctgcgaata
    11761 gtccgcgtcc acgagcacgt tgccgggcgc ggcgacaaac attttgcgca gtcccgcccc
    11821 cagctcgcgc cggacgggga tgttctgcaa attcggctcg gtggacgaga ggcgccccgt
    11881 cgccgtgacg gtcatctgga aattggtatg gatgcgtccg tccgcgccga tgaccttcaa
    11941 aaggccgtcc gcgtaggtgg atttgagctt ggtgagctcg cggtattcga gcacgtggcg
    12001 cacgatgggc tcatagcgga gcttatcgag cacatcggca ttggtgctcc agccggtctt
    12061 ggtctttttc ccgccgggga gaccgagctt ttcaaaaagc acggcgccga gctgcttcgg
    12121 ggactggatg ttgaattcct cccccgcctc cgcccaaacc gcttcgcgca gcgcgtcgat
    12181 gctgcccgta agactctcgc cgaaggcgcg cagcgcctcc gcgtcggcaa gaaagccctc
    12241 gttttccatt ttggcaagca ccgcgcagag cggcagctcg atctttgtaa aaagctcctc
    12301 catctgcgtc tcgcgcagct tcgggagcat aacttcctcg agcgccgcga cgcccgccgc
    12361 gcgcgaggcc atctcgccga gccggtcggc gagcgttttt ccctccccgc cggagaggtc
    12421 gagcatgagc tgatcgccgc tgtccgccgc gccggacgag gtatgcggcg tgaagccgca
    12481 gtaggcgagc gtgagctttt cgatctcgta cccggcggca gtcgcgtcga gaagataccc
    12541 ggcgagcgcg ctgtcgaaca cccagttttc gagcgcgtcc actcctcgcg cgagcagcgc
    12601 gcgctgcaga ttcttcacgt cgtgccccgc cttgcggcgc tcgggcgcaa agagcagcgc
    12661 gagcgccgcg gtatagccgt cccggtatgc ctccggcgtc agaaataccg cgagcgcgtc
    12721 attttcggaa acgctgaaat gcgcgagcgt ctcgccgctt tcggcatcaa accagtaata
    12781 ggcgacggcg tcccccgccc gctccagcgc ggcgcggagg tctttttccg tttgcggcgt
    12841 cacgctctcg catgtgccct caaatacggt ctcttccttt ttctcctccg cggccggctt
    12901 caagccccac ttttcaatga acttctggaa gccgagccgc cggaaaattt catatgccgc
    12961 gccgccgtag tctgccgggc ggcgaagctc gtcgggggaa atatcgatgg gtgccttgcg
    13021 gtcgatcgtg gcgagatagt acgaatcctc agcgctctct ttgccgtttt cgagctttgt
    13081 gcgcagcgag gcgcggatgt cgggatcgct gatatgctcg tacacgcccg cgagcgagcc
    13141 gtacttccgc acaagctcga gcgccgtctt ctcccccacg ccggggacgc cggggatgtt
    13201 gtcgctcgtg tcgcccatga gggccttgag atcgaccatg tgcggcgcgt caaagccgta
    13261 ttcctcgcgg aagcgctcgg gcgtatagtt gacggtctcg gtctggccca tacgcgtttt
    13321 gacgttgagc acgcgcacat gctcacccac gagctgcaaa gcgtccttat cgccggtgac
    13381 gacgacgcac tcccagccct gctccgctcc gatgcggctc acggtgccga gaagatcgtc
    13441 cgcctcccag ccggagagct cgcgccgctc gatgccgagc gcatcgagca cgtccttcaa
    13501 aaccggcatc tgcgcggcga gatcgtccgg catgggcttg cgcgtcgcct tatacgcctc
    13561 gctgtgcttg tggcggaacg tcggctcgcg gcggtcgaac atgacgcaga gcgcgtcggg
    13621 cttttcgtcg tcgagcaggc gctgcaatat attcagaaag ccgaaaacgg cgttcgtcgg
    13681 cgtgccgtcg ggcgcggaga gcgggcgcac gccgtaatac gcgcggttca cgaggctgtt
    13741 gccgtcgatg agcatcagtt ttggcataga gtttcctcct taagttcctt ggcaagcggg
    13801 cggtacacgg cgagcataag cgttgtgaac gccgcgcccc cgccgagcag atcgctgatc
    13861 ggctcgctcc agtacacgcc gtccgccccg agaccgaata cgcccgggag cagcagcatc
    13921 agcggcacga cgagcacgat cttgcgcagc agcgaaaaga agatcgcctg ccgcgccttg
    13981 ccgagcgagc ggaacgagtt ttgcgcaacg aattggagcg acatgaacac gaacccgcag
    14041 aaataaatgc gcacggcgcg caccgccacg gcgatgagcg cctcatccgg cgtaaagatg
    14101 cggatgagcg gttcggggaa gagcatgaac accgcccaca cgagcagcga aaacgccgcc
    14161 gcgccgagcg tgctgaaccg gatcgtctcc agcacgcgct ttttctgccc cgcgccgtag
    14221 ttaaaagcga gcaccggctg gatgccggag ccgaagccgt ggatgatctc ggtgaagatc
    14281 gtccgcagcg agtttacgac cgtgatcgcg ccgacataaa gatcgccgcc gtacagccca
    14341 agctggcggt tcgccacggt ctgaatggcg gagttcgtcg cgcccatcac gaaatcggct
    14401 acgccgagag acatgatgcg tcccatgatc ttcttccgcg gcgccatcgt gcgccacgaa
    14461 agctcgagcg gcgttttgct gctgcgcaga aagaggaagc accacgcggc gctcagcgcc
    14521 tgtgagagca ccgtggcagc cgccgcgccg cgcacgccca tgcggaaaac aaaaatgaac
    14581 agcgggtcga gcacgatgtt cgccgccgcg ccgagcagca ccgtgagcat gccgcgcgtg
    14641 gcgaagccct gggcgttgat atacacgttc atgccgaggg atacgaacga gaacgtcgtc
    14701 ccgatgagat acactgcgag atactcgcgc gcataggagc ttgcctcctt gcccgcggcg
    14761 aacaccgtta tcaccggatc gagaaacgcc catgtgagca gcccgagcgc aacggacgaa
    14821 acgagcagca tggagaaggc gttgcccatc acgcgggagg cctcgccgtt atccccgcgg
    14881 ccgcgcgcca tggagcaaag cggcgcgccg ccctgtccga acaggcccgc gaacgccgag
    14941 aggatcgaaa tgaccggcag cgcgatgccg acgcccgtga gcgccatgct gccgacatcg
    15001 ggaatatggc cgatgtagat acggtccacc acgttgtaga gcacgttcac gagctgcgcg
    15061 gcgatgagcg gcagcgccat gcgcatgacg accgcgggga tattcccttt ggaaaaatcg
    15121 ttttgtacgt tcttttccgc catggtcact tctccccgcg cagccggatg atctgatcgc
    15181 gcagcacggc ggcatattcg tattcgagca tctgcgcggc tttgcgcatt tctttttcca
    15241 gccggtcgat ctcggccttc cgctcgcgct cggtcatgcg catgccgcgc tcgtccttcg
    15301 ccgcggggcc ggtaatctcg agaagatccc ggatgctctt tttgatggtt ttcggcacga
    15361 tgccgtgcgc ctcgttatac gccgtctgga tggcgcggcg gcgctcagtc tcgtcgatgg
    15421 cggcgcgcat ggagggcgtg accgtgtcgg cgtacatgat gacggtgccc tcggcgtttc
    15481 gcgccgcgcg gccgatcgtc tggatgagcg atgtttcgct ccggagaaag ccctccttat
    15541 ccgcgtcgag gatcgcaatg agcgatacct cgggaaggtc aaggccctcg cgcagaaggt
    15601 tgatgccgac gagcacgtca aattcgcccc ggcgcagatc gcggatgatc tccatgcgct
    15661 ccatggcgcc gacgtcgtgg tgcatatagc gcgttttgat gccggcgttg ccgagatacg
    15721 ccgttaagtc ctccgccatg cgcttggtga gcgtcgtgac gagcgtgcgc tcgccgcgct
    15781 cgatgcgcgc gtggatctcg gcgatgagat cgtcgatctg tccctcgatg gggcgcacgt
    15841 cgatcctcgg gtcggtgaga cccgtcgggc ggatgatctg ctcggcgatc tggcccgagc
    15901 gcgtgcgctc gtactcgccg ggcgtggcgg aaacgtatat gacctgattt acccgctgct
    15961 ggaattcctc aaacttcagc gggcggttat cgaacgccga cggcagccgg aagccgtatt
    16021 caacgagcgc ttccttgcgc gcgcggtcgc cgtggtacat ggcgcgcacc tgcggcagcg
    16081 tgacgtgcga ttcgtcgatg aacaggagaa agtccttcgg gaaatagtcg agcagcgtca
    16141 tcggcgcgct cccgaccggg cggccggaga tgatgcgcga atagttctcc acgccggagc
    16201 agtagcccag ctcctggatc atctcgatat caaaattcgt gcgctgcgcg atgcgctcgg
    16261 cctcgagcgg cttttcgtgg tctttgaaaa acttcacccg ctcgtcgcac tcgcggcgga
    16321 tctcctgcac ggcgcgcgcc attttctccg gcggcgtcac gtagtggctc gcggggtaaa
    16381 tggccgtgtg gctgagcgtg tgcaccgcag cgcccgtgag agggttgatc tcgctgatgc
    16441 gctcgatctc gtcgccgaag tattccacgc ggatggcctt atcgcgccag taggccggga
    16501 agatctccac cgtgtcgccg cgcacgcgga acatgttgcg ctcgaaggcg atgtcgtttc
    16561 gctcataccg gcagtcgata agctttttca gcagctcctc ctgcggcaga ctgctgccgg
    16621 ggcggagaga aatagccatg cgcgcaaaat cgtccggctc gccgagaccg taaatgcagc
    16681 ttaccgacgc cacgacgatc acgtcccgcc gctccagcag cgaaaacgtc gccgaaaggc
    16741 gcagccggtc gatctcctcg ttcgtagagg cgtccttttc aatgtaggta tccgtgtgcg
    16801 ggatgtacgc ctccggctgg tagtagtcgt aatacgatac aaaatactcc acggcgttgt
    16861 ccggaaagaa ttccttgaac tcgctgcaaa gctgcgcggc gagcgttttg ttgtgcgcga
    16921 gcacgagcgt cggcttctgc acgcgctcga tgaccttcgc catcgtgtac gttttgccgg
    16981 agcccgtgac gccgagcagc gtctgctcgt ccagccccgc ggccacgccc tcggcgagct
    17041 tttcgatcgc ctgcggctga tcgcccgcgg gcgaatattc gcttaccaca tgaaattccg
    17101 gcataacgcg ctccttttaa aataatttct ataattgatt tattatacca cgccgcgccg
    17161 cgccgcacaa gcgattcgcc cgccccgtgc gtaaaaactc cctgcccggt ggatttccgc
    17221 cgcgggatat gctatactga caatataatg attttaaggg gggtgcttcc ctatgaataa
    17281 aactctgatc gcgtatttct ccgccagcgg cgaaacggca aagcttgcga aaacgctctc
    17341 cgccgtgacc ggcggcgacc tttttgagat ccgtcccgag acggcgtaca cctccgccga
    17401 tctgaactgg atggacaaga agagccgcag cacgctcgag atgaaggacg agcacagccg
    17461 cccggccatt gcgggccgcg tggaggatat ggcgcagtac ggcaccgtgt tcgtcggctt
    17521 cccgatctgg tggtatcagg cgccgcgcat catcgaaacg tttcttgaaa gctacgattt
    17581 caccggcaag accgtgatcc cgttcgccac ctccggcggc agcggcatgg gaaagacggc
    17641 cgatattctg cgcactgtct gccccgcggc caacattctg cccggcaagc gcatgagcgc
    17701 gcgggagagc gccgaggccg tgcgcgcgtg gctcgaaacg ctcagaaagt aaaaggaaaa
    17761 agcagggagg atcatcccat gggaagaaaa gaagcgccgc gccgtgtcgc cgtgatcggc
    17821 ggcggcgcgg gcgggctctg cgcggcgatc gccgccgggc aagccggagc ggacgtggcg
    17881 atttttgaac aggcgaaccg cgtcggcaaa aaactgctca agaccggcaa cgggcggtgc
    17941 aatctctcga atacgcacgt ttcgccggag cactataacc ggccggattt cgtcgcgccc
    18001 gttcttgcgg acatgccgtg cgatgcgctg cgctctttct ttggctcgct cgggctctgg
    18061 acggtcgcgg atgaagaagg ccgcgtctat ccgcgcagcg acaccgccgc ctccgtgctc
    18121 gacgtactgc gtctcggctg cgaggcgctg ggcgttgcgg agcgctgctc ggcggagatc
    18181 gccgccatcg ccccgcaggg ccgcggcttt accctcaccg cccgcacggg cgagaagttt
    18241 tttgccgacc gcgtgatcgc tgcggccggc ggcggaacga gccttctccg tccgctggga
    18301 cacaagatga tccccttctc tcccgttctc tgcccgctca agaccgacac ggaatccatc
    18361 cgcggtctca cggggctgcg ctgcgcctgc cgcgcggagc ttatccgccg cgggaagagt
    18421 gtttacaccg aagagggcga aattctcttc cgggatttcg gcgtgagcgg catcctcgcg
    18481 ctgaatctct cgcggcatgc ccttccgggc gacacgctct cgcttgatct gctgccggag
    18541 cttactttgg aagcgctcgc cgcgaagctc tcggcgcaga aggcgctgcg cggagctgac
    18601 gccgatctct tcaccggcat tttccaccgg cggctcggcg aggcggtagc gcgccgcgcc
    18661 aaaagcggcg agatatccga gcttgcccgc attatcaaaa attactgtct ttcggtcctc
    18721 ggtccgggcg acacgcagca cgcgcaggtg acgcgcggcg gcgcggacgt ctccgagttt
    18781 gacagcgaaa cgatggagag ccgccgcgtg ccggggctct atgctgtggg tgaggcgctc
    18841 gacattgacg gcgcgtgcgg cgggtacaac ctgcactggg cgttcgcctc cgggctgcgc
    18901 gcgggaagga gcgccgcgcg tgattgaggt tcgtgatgta aggctcgctc ttgacggtga
    18961 tctcaagacc gcgtgcgcca aaaagctgcg cgttccggtt ggcgacgtgc tggaagcagc
    19021 gctcctgcgc cgcagcgtgg acgcgcggcg caaggacgac gtgcatttca ccgtgaccgc
    19081 cgcggtatcg ctccggcggg gcgaggaaaa gttttcgccc tatacgccgt ggacgccgcc
    19141 gcgcgtcgaa gttttgaaaa agaagcccgc ccttcgcccc gtcgtgtgcg gggcaggccc
    19201 ggcggggctt ttcgccgcgc tcacgctcgc gcgcgcgggc gcggagccga tattgctcga
    19261 gcgcggcagc gccgtggacg agcgaaaggc cgacgtggaa aaattctatc agacccgcgc
    19321 gctggacacc gaatcgaaca tccagttcgg cgagggcggc gcaggcgcgt tctcagacgg
    19381 caagctcaac accggcacgc acgataagcg cggccggcag gtgctgcacg atctcgtaca
    19441 ggccggagcg ccggacgaaa ttctctggca ggccaagccg cacatcggca ccgaccgtct
    19501 gccggacgcc gtgaaggggc ttcgtgaaat gatcctcgcc cacggcggcg aggtgcgctt
    19561 ccgcacaaag ctcacggata tcgagctttc cggtgaggcg ctgcgcgcgg tgcgtctcgc
    19621 gcacggcggc gcggaggaag cgctcgaggc cgattcactc atccttgccg cgggccacag
    19681 cgcccgcgag ctgttcgcgc tcttgaaaga gcgcggcgcg cagctcgaac agaaggcgtt
    19741 ttccatgggc gtccggtgcg agcacccgca ggaggtcatc tcccgcgcgc agtacggcgc
    19801 ttttgccgct cacccggcgc tgggcgctgc cgactaccgg ctggcggtgc atctgccgga
    19861 cgggcggggc gtatatacgt tctgcatgtg tccgggcggg tacgttgtcg gcgcggcgag
    19921 tgaagaggga cttctctgcg tgaacggcat gagccccttt gcgcgcgacg gacagaacgc
    19981 caacgccgcc gttttgtgcg aagtgcgccc cgcggacttc ggctcctccg atccgctcgc
    20041 cggtgtggaa ttacagcgga ggtgggagcg cgccgcgttc ctcgccggag gcggcgatta
    20101 ccgcgccccg gcgcaattgc tcggcgattt cctcgccgga aagccgtcgg agcgcgcagg
    20161 cagcgtcacg ccgacgtatc cgctcggcgt gcgcttcggc acgctggatg gctgcctgcc
    20221 ggagttcgtg ctcgccgcgc tgcgcgaggc gttcccgctt tttgataaaa agctgcacgg
    20281 gtacgccatg ccggacgcag tgctcacggg cgtggagacg cgctcatcct cgccggtgcg
    20341 catcgtgcgc ggcgaaacgg ggcagtcgaa tctccgcggg gtctatccct gcggcgaggg
    20401 cgcgggctac gccggcggca ttctctccgc cgccgtggac ggcatccgtc aggcggaaaa
    20461 ctggctttcg cagcacatgg aataaaataa cagacaccgc cgcgcatacg aggcttttga
    20521 taagccaagc tatgcgcggc ggaacctttt ccggcagagc cggaatcggc tctgcttcaa
    20581 tatagatggg cgggacagct ttcgctgtcc cgcttcctct ttttagggtc gatccttcat
    20641 tctcgttttc atggcaaaaa gccctgcggc ggcaaggaaa cacggaacgc caaaaatcag
    20701 cagcagcctc acggcggaat caaacacagg cattttatag atcacaacgt cctccggctc
    20761 ttccatgcga actatgcacg gccgcagaaa ccggtttttc atccggtacg ccgtgccgtc
    20821 cgccgaaaca tactcataca gcaccgacac gctcaatccg tcgccgtgga tctctctgct
    20881 ttctatccgc gcgccgaacc gatccgtccg gtataaatac ccaccggtgt cgatccaaag
    20941 cgtttgatcc gcatccatcg tgaatcggta gcctctggag gtgcaggggt cgatccgccg
    21001 cagcacatcg ccgttcggtt tcaggacctc gatcacctga gtcttcccta tacagattcc
    21061 cgtcgccgtc cacggcaaac ccgttataaa acaggcaggc tttccccgtg accgcagaaa
    21121 cggaacaaaa gatcacggca attcctgcga tcaccatgca caggagcatc catcggaatt
    21181 tttctttatg tatcattacc gttcgacctc aggctttatt ctgcatgaat ggctcgttct
    21241 cgttggtctc attatatttt ccgcaacggg agattgcaat accgtacctg cgcaaaaagc
    21301 cgcttctctc ccgaatggga aagaagcggc tttttgctta tgcctccctc aaagagggag
    21361 ccctatatca gaacttgacg cgctcggcga tgtagtcctt cacttccgcg atggggatgc
    21421 ggacctgctc catgctgtca cgctcgcgta ccgtgacgca gccatcggcg ggcgttttct
    21481 cgtcgcccac ggtctgaaag tccacggtga tgcagtaggg cgtgccgatc tcgtcctcac
    21541 ggcggtagcg cttgccgatg gaaccggcgt cgtcgtagtc caccatgaat tccttctgca
    21601 gctcgtggta aagctcggtc gcggcggggg cgagcttctt cgagagcggc agcaccgcgc
    21661 acttgaacgg cgcgagcgcc gggtgcaggt gaagaacggt acgcacgtcg tttttcgcct
    21721 cgtccacgac ctcttcgtcg taagcgtcgc agaggaacgc gagcgccacg cggtcgcagc
    21781 cgagcgacgg ctcgatgacg tacggcacat agtgctcgtt cgcttcctgg tcgaagtacg
    21841 taagatcctt gccggacgcc tcctgatggc gcttgagatc gtagtccgtg cggtcggcaa
    21901 tgccccacag ctcgccccag tcggtgaacg ggaaggcata ttcgatgtcg gtcgtggcgc
    21961 gggagtagaa cgcaagctcc gccggctcgt ggtcgcgcag ccgaagatgc tcttccttga
    22021 tgccgagact cagcagccag ttcttgcagt agtctttcca gtaggcaaac cattcaaggt
    22081 ccgtgccggg cttgcagaag aattcgcact ccatctgctc aaactcgcgc gtgcggaacg
    22141 tgaagttgcc cggcgtgatc tcgttgcgga acgccttgcc gacctggcag acgccgaacg
    22201 ggagcttccg gcgcgtcgtg cgctgaatgt tcgagaagtt gacgaaaatg ccctgcgcgg
    22261 tctccgggcg gagatagcag gtcgaggagg agtcctcggt aacgccgatg gcggttttga
    22321 acatgaggtt gaacttgcgg atgggcgtaa agttgtgctt gccgcacacc gggcagacga
    22381 tgtcctcgtg ctcggcgata aaagcgtcca tctcctcaaa cgtcatggcg tccacgttga
    22441 cctcgtggtg ctcctcgccg atgagcttgt cggcgnnnnn nnnnnacgtg accggttgtg
    22501 atccaggtct gcgggttcat gatgatggcg gcgtcgagac cgacgttata ggggctctcc
    22561 tgcacgaact tcttgagcca cgccttcttg acgttgttct tgaactccac gccgagcgga
    22621 ccgtagtccc aggagttggc aaggccgccg taaatttcgc ttccggcgta gacaaagccg
    22681 cggttcttgc agaacgctac gatcttgtcc atgggttttt cgttatgttc catttttctt
    22741 tnnnnnnnnn ncccagatag tgtaccacgc cgcggcccga tgtacaagcc gcggcgtgga
    22801 tttttcttta tcggataaac cggtcgttgc gcgaaacgat cttcacgcgg cggcttgccg
    22861 cgaggaacac aacgatgatg aacacgataa agatgaggcc gctgtagtca aagtcgagcg
    22921 aggcgagaaa gtcgtacatg ccgtggatca gcacgggcag ccacagcgcc cggcggcggc
    22981 agacgcgctc gccgataaaa tcgccctcgt tcgcgtagcg ccgcgccgcg ccgtaccaga
    23041 tgcccatgaa aacgccgaag caggcgtggc ccggcaccgc cgtcaccgcg cgcacgagcg
    23101 ccgtgccgaa gccgtacatg gcaacatacc cgatgttttc ccacagcgca aagccgagcg
    23161 agacgaacac cgcgtacacc acgccgtcaa actggcagtt gaaattcggg ctgcgccacg
    23221 tgcgccagcg gagcagcagc agcttgaagc cctcctccga gagcgccaca acgatgaagt
    23281 tcgataggat cagatagccg aggtcggtct cgtcaaagag cgagagcagg atgttctcgc
    23341 cgatcgtctc ggtcacgatg gcgagcgccg tggcgatcac gccgaaaacg agcagcgaga
    23401 tcaaaagccc cgtcggttcc ttttccagtc ggtcggcacg gtagacctgg agcagcaata
    23461 tcaccgccgg gatcaccgcc gcggcaatca gcacgggatc catttcgctt atggtagaca
    23521 gcatagcgtt tccctctctc ttacttcttt cttctttact ctttttcatt tttatagcat
    23581 acgccggggc ggattgcaag gaactttcaa agcacttgtt atcggataat gtgcttgata
    23641 taatggtgaa aaggctccct tgtgcaaagg gagctgtcag ccggcaggct gactgaggga
    23701 ttgttctttt ccagaaagct gcgttgtcta caatccctcc gtcaaaacct ccggttttgc
    23761 cacctccctt tacacaaggg aggcttctat aaagaatttg ttttaaggag ggagcgccat
    23821 gctgcggctg attttgggcc gtgccggaac gggcaaaacc gcgcttctgt tttccgagat
    23881 cgcggcgcgc tgcgcccgga ggcagtccgg cagctttctc atcgtacccg agcagtacag
    23941 ccacgaggcc gagcgcgagc tcgcgcagcg ctgcggcgat acggcgagcc tctatgccga
    24001 ggtgctttcg ttctcgcgcc ttgcccaccg cgtcgccatc ggctgcggcg gcagcgcgcg
    24061 cgtttacatc gataagcccg gacggttttt acagctctcg ctcgcgctcg agcaggtggg
    24121 cggcgcgctc agcgtatacg gcggcagcgc ccgctcgccc gagcgcatgg tacagctcct
    24181 ctcggcgctc gatgagcttc gcttcggctg cgccgcgccc gatgcgctgc gcaaagccgc
    24241 cgaaagcgcc acgccctcgc tcgcgggcaa gctgcgcgat ctcgcgcttt tgcagtccgg
    24301 cgccgatccc gccgggcggc tcgacgtgct ggccgagcag cttcccggct gcgcatggct
    24361 ccgcagcgca aagttttata tcgacggctt cacggacttc accgcgcagg agcgccgcgt
    24421 catccgtgcg ctgctgccgc tcgcggacgt gacggtctgc ctcacctgcg acgatctcgc
    24481 ctccggcagc gaggtgttct ccctcgcccg ccgcacgatg cgcgcgctgc gcgacgccgc
    24541 cgaggaggac ggcgtggccg tagagatcgt ctccgtgccg cgcggcgaag atcgcacgcc
    24601 ctttgcgttt cttgaggaga atctcttcgg ctggacggac gagacgcgcg acgccgcagg
    24661 gagcatccgg ctcgtgcgcg cacccggtgt cgcggcggag tgcgagctgg ccgcggcaga
    24721 gatgcggcgg ctcgtgcgcg aaacgtcctg ccgctggcgg gacattgccg tcgcggtgcg
    24781 cggcttcggg gattaccgcg ccgcgctcga ggaggcgtgc cgccgcgccg gtgtgccgct
    24841 ctacgcgagc gaacgaacca acattttcgc ccgtcccctt ccggcgctca ttgccgcggc
    24901 atttgatatt cttgccgacg gctggagcta cgagagcgtt tttacctatc tgaaaaccgg
    24961 tctgaccggc atttcccgcg ccgattgcga cgcgctggaa aactacgttc tgctctggtc
    25021 gctgcgcggc acggcatgga cgcgcgccga gccctggcgg cagcacccgg acggcttcgg
    25081 cggcacggtc acgccggaga gcgacgcgct ccttgcaaag atcgacacgc tgcgccggag
    25141 cgtcgccgcg ccgctcgcgg cgttcgagcg ccgcggaaag gaagcgaccg acgcgcgcgg
    25201 acagtgcgcc gcgctcgcgg cgttctggga ggatatttcc ctgccggagc atctggagga
    25261 gcgctctctc gcgctcgagg aggccggaga gcttcaggct gcggcggaat accgtcagct
    25321 ctgggagctg atcgtttccg cgctcgagca gtgcgaggcc attctcggcg acatgccgct
    25381 ctcgcaggag gatttcgggc ggctcttccg ccggatgctc tcgcaatacg acgtcggcac
    25441 gatccccgtg gcgctcgacc gcgtgaccgc cggagacttt gaccgcatgc ggcggcgcag
    25501 catccgccat ctgctcgtgc tgggcgcgtc cgacgagcgt ctgccgcgcg tttccgacgg
    25561 gggcggcgtg ttcaccgaca ccgagcgcag cgagctgcgc gcgctcaaca tcgaactctc
    25621 cggcggcgac gacgagctcg accgcgaatt caatctcatt tataacgtat tcacgcttcc
    25681 caaatcgtcg ctctacgtca gccgcagcgc gtttgcctcc ggcgagagcg agacgcgccc
    25741 ctccttcgtc accgaccgga tcgcaaagct gttcgcgctt tgcgaagaga acggcgacgt
    25801 gcatgccgcg agagcgcagt cgctctccgg cgcgctggag ctggcctgcg ccggagacgc
    25861 cgcagcccgc gagtttttcg ccgcgcgcgg cgagagcggg cgcatcgccg ccatcgaaaa
    25921 ggccgccgcg gaggaacgcg ggcggctctc ccgcgagggc gtgcgcgcga tctacgggca
    25981 gaagctctat ctcacggcct cgcgcatcga caactttgcc tcctgccggt tccagttctt
    26041 cctgcgttac ggtctgtgcg ccaagccgcg ccagagcgcg cagtttgccc cgccggagcg
    26101 cggaacgttt ctgcacttcc tattggaaaa cgtggcgcgc gaggtgtcgg agcgcggcgg
    26161 ctttgccgaa acggacgaca agaccgtcgc cgcgctgacg gacaaatacg tccgcgaata
    26221 tgtgcgcacg cagctggagg attttcggga aaagagcccc cggttcgtct atctcttccg
    26281 ccgtctggcg gagacatcgc ggcgcatcgt gctcgacacg gcgcaggagc tgcgccgctc
    26341 cgacttccgc ccgctcgact ttgagctgaa tttctccaac aaggacgacg ccgatctgcc
    26401 gccggtcagc gtcggcagcg gcgaggacga atttgttctc accggcacgg ccgaccgcgt
    26461 ggacggctgg gagcacggcg gcaagctcta catccggatc gtggactaca agtccggaaa
    26521 gaaaaaattc gcgctctccg acgtgtggca gggcatgggg ctgcagatgc tgctctatct
    26581 cttcacgctg gagaagcacg gcgccgagcg ctacggcaag gagatcgtcc ccgcgggcgt
    26641 gctgtatgta ccggcgcacg atatccttgt gcgcgcggac aagcgcctct cggacgagga
    26701 gatcgtggcg gagaaggcca gaaagctgca ccgcagcggt ctgatcctgt cggaaaccga
    26761 ggtcgtcgag gccatggagc acggcgaagc gctgcaattc cttccgctgt accgcggcaa
    26821 gctcgccgcg gaatcgctcg ccacagccga gcagctcggc aagctctcgc ggtttctcga
    26881 ggagacgctc tcgaacatgg ccggagagct ccgcggcggc agcatcgccg ccgacccgtg
    26941 gtatgaaaac gacagagcca acacctgcnn nnnnnnnncc gaaagccgcg gcgtagcgcg
    27001 gctcgtttcc gccgctgcgg gctccggcaa aacaaaggtc ctcgttgagc ggcttatgcg
    27061 ctacgtagac cgcggcgcgg acatcgatca gtttcttgtc atcacctata cgcgcgccgc
    27121 ggccgccgag ctgcgcagcc gcattctctc cgcgctcagt gagcgcattg ccgccgaccc
    27181 gatgaaccgc cgtctccggc ggcagacgga gctgtgctgc cgcgcctcca tcggcacgat
    27241 cgacagcatc tgcggtcgtt ttctgcgcga gaacacacac ctcgccggca tctcgccgga
    27301 ctaccgtgtc atcgagccgg accgctccga cgagatctgc gcccgtgtgc tcgataagct
    27361 cctcgaagag ctgtatgaga ccattgaaga ggatgcgggc gtacgcgcgc tcgtggattc
    27421 gttcggctcg ggagacaacg acgataagct ctcggcgctt gttttgcggc tgtacaaatc
    27481 gctgcagagc cacgccgacc ccgatgcgtg gctcgccgag cagcgcgaac ggctcaccga
    27541 cgcgtggaac gacgtcggcg atacgccgtg ggggcagtat cttctctccc gcacgcgctc
    27601 ccaggcgcag tactgggcgg agcagatcga ggagctccgt ctccggctgg acgagccgga
    27661 tcgggacgcg aagatcaaaa aggcgtacac caataatctt gatttcgccg gtgacgccgc
    27721 gcgggacatg gcgcgcgccg ccgcgctcgg ctgggataag gcgcgtctcg cctgccccgg
    27781 ctctttccct cgccgcggta tctataaggg cgacgacccg ctcgcgcagc ggatcatcgc
    27841 cgtttggacc ggcttcaagg acaccgccgc aaaatggcag gagcttttcg ccaacgattc
    27901 cgacgcgctc ctgcgcgatc tggccgtcaa cacccccgcc gttgacgcgc tgctcgcgct
    27961 gacgcaaaag cttggcgccg ccttcgccgc cgagaaaaaa aggcagggcg tgtgcgactt
    28021 ctccgatcag gagcatctgg tgctcaagct gctcacggac acgtccaacg gcctcgccgc
    28081 cgcgctttcg agccgctata cggaggtgct cgtcgatgag tatcaggacg tgaacgcctg
    28141 tcaggacgcg ctcttccggc tgctttccga caacgggcgc aagcttttca tggtcggcga
    28201 cgtgaagcag tcgatctacc gcttccgtct ggccgacccg acgatcttcc tgaaaaaata
    28261 cgagacctgg ccggacatcg aaacggcgga gccacgcgaa gagccggggc gcatcctgct
    28321 gcgggaaaat ttccgctcgc gccccgaggt actcgagagc gcgaaccatg ttttccgcaa
    28381 catcatgtcc cgcgagctcg gcgagctcga atacgacgag gcggcggcgc tccggcccgg
    28441 cccggggcgg gatttcccgg agggcggcgg cgcgccgacg gagttctgca ttctcacgcc
    28501 gccggacggc ggtgacgacg aggatgacgc cgagaacgac gaacgccccg ataaggtgct
    28561 gcatgaggcg cggtatatcg cctcgcgcat ccggcgcatg gtggacgagc gcacccccgt
    28621 caccgaggat aagacgctgc ggccgtgcaa ctacggcgat tttgccatcc ttctccgctc
    28681 gaacaaggcg gtcacgccgc gctaccgtgc cgcgctcgcc gagcagggca tcccgtcggt
    28741 gtcccagcag ggcggcgggt ttttccgctc cacggagatc acctcgctcc ccgcgctgct
    28801 cggcgtgatc gacaatcccc atcaggacgt gccgctcatc gcggtgctgc gctcgcctct
    28861 tttcggcttc acgccggacg agctttccgc catccgcgcc gcggaccgaa acagcgacta
    28921 ttacaccgcg ctctgtctcc gcgcaaagga ggatgaacac tgcgcggcct tcctcgcgga
    28981 gcttgagagc taccgtgaga tcgcgccgga cctttccatt gaagcgctgc tcgcgcgcat
    29041 ctgcgacaag gccggtgtct tcgccctgct cgcagcgatg cccgacggtg cggcgcggcg
    29101 cgagaacgtc tgccagctca tcgagttcgc ccgccagttt gagcaggacg gctatcgcgg
    29161 cgtgttccgc ttcatccgct ggatgaagca gctggatgag cacggcaaag agccgcgcac
    29221 cggtgcactg gaaagccgca acgccgtgca gatcatcagc atccaccact cgaaggggct
    29281 ggagtacccg atcgtatttc tcgccgacac cgcccacaag atcaacctgc aggacaggaa
    29341 cagcgatgtt ctcatccacc cgtcgctcgg catcggcggc cgcgtcatcg acagttcacg
    29401 cggcatatcg tatcccacga tcgcactgcg cgcgatctcg gccaagctca cgcaggaatc
    29461 gctgtccgag gagatgcgcg tgctctatgt cgccatgacg cgccccaagg accggctcat
    29521 catcacagcg acatggaaaa ctcccgaaaa gacgctcgac gcgctgcgcg acgatctcac
    29581 cgcccccatc gcgccgcagc ttctcgaaaa gagcgcctcg ctttcgcgct ggatcgcgct
    29641 cgcggcgctt cttccggaca gcccgatcaa aatgcgcatc attcccgcct ccggagagag
    29701 cgcgtccgag accgcgccgc ccgagatcga actggctgag ccgggcgaga gcgcggcgct
    29761 ctccgaggcg ctgacgtggg aatacccgta ccgctgggcg gaggagctgc cgtcgaagat
    29821 caccgcgagc gcgcttgagg gcaccgacgc cgacccggac gcgcagagca tcgcgccgga
    29881 agcacagcgc cggcggctct tccgccgtcc ggtcttcggc gcgaaggacg ccccgctcac
    29941 cggcaccgcg cgcggcatcg ccgtacacac ggttctgcag tttattgact attcgcgcgt
    30001 atccacgccg gagcaggtgc gcggcgagat cgaacggctg aaaaatgccg agcacatcac
    30061 ccccgcgcag gccgccgcag tggagccaaa ggacatctgc aagctgttcg cctcccccgt
    30121 cgggcagaga atcctgcacg cggacgaggt ctggcgcgag ctgcgcttct cgctgctcac
    30181 cggcgcggag accgtgttcg acgcgcccgc cggcgaggag gtgctgctgc agggcgtcgc
    30241 cgactgctgc atccgcgagg gggatgcgct caccgtcgtg gattataaaa cggactatgt
    30301 gacggcggaa acgctcgcgg cgcgtgcctc ggaatacgcg ccgcaagtcc gcgcctacgc
    30361 cgccgcgctc acgcgtctgc tcggaaagcc ggtgcgggag ggcgttttgt tcttcctgcg
    30421 caccggggag agcgtctcca tcgactgccg cgcgaaaaaa taaaagaaat tcaaagaaaa
    30481 cttgccgatt ccgcttgaca ttcgccggaa attgggtata ataacccttg cgtttgcgat
    30541 atagcgggat tgtgtaaagg tagcacagcg gactctgact ccgtatgtga gggtt
//
LOCUS       DAWAAE010000005        29469 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_683_length_29469_cov_2.983341, whole genome shotgun sequence.
ACCESSION   DAWAAE010000005 DAWAAE010000000
VERSION     DAWAAE010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 29469)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29469)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29469
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_683_length_29469_cov_2.983341"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6655..6664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9792..9801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11484..11493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22433..22442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5752 a         8802 c         8607 g         6268 t
ORIGIN      
        1 ccactcggcg gccttttacc gcttcatcgc ctccgtcatg cctgaccacg aggcacgccg
       61 cgcgctgctg cggcagtaac gaaagataag gagacctctc catgcacatt ccgaccgccg
      121 acaccgatac gctcgagctg ctgccgttcc gcgaggcgct ggaaaagagc ggacagcgcg
      181 gcgattatga cgccgcgcgc tggctgtacg ttccggattt ttataccgag taccgataca
      241 ttctcggcac gcgcggggaa aagccgctca tctgcatcgg cgtgaatccc tcgaccgccg
      301 cgccggacgc cctcgacccg acgctccaga gcgtgcagcg catcgcgctc tcaaacgggt
      361 acgacagctt tatcatgttc aatgtctacg cccagcgcgc cacgcgcccg aacgacatgg
      421 acgccgcgct caatgagcat ctgcacgcgg agaatatggc ggcgttccgg tacgttctcg
      481 aagcctctct cccccgccgc ccggcggtat gggcagcctg gggcaacatc attgaaatgc
      541 gcccgtatct cccgcgctgc gtttcggaca tgatcgccgt cggcgaggag cttggcgcgg
      601 agtggttcac ggcgggcaag cgctccgtaa agggccatcc ccatcatccg ctctatctgc
      661 ggcgcgatac gccgctcgac ccatttgacg taaaatccta catagataca ctggaaggaa
      721 ggactcatca tgcagaactt tgaatactgc gttcccacga aggtcgtttt cgggcgcgac
      781 accgaaagcc gctgcggtga gcttgtccgc gaatgcggcg gcacgcacgc gctcatcgtt
      841 tacggcggcg gcagcgccgt gcgctcgggg cttctcggcc gctgcaccga ttcgctcaag
      901 gccgcgggta tcccttacga gctgctcggc ggcgtgcagc cgaatccccg gctcagccgc
      961 gtgcgcgagg gcctcgcact tgcaaaggag accggcgcgg actttctgct cgccgtcggc
     1021 ggcggcagcg tgatcgacac ggcgaaggcc atcggctacg cgctcgccaa tcccgaattt
     1081 gatatctggg atctcttcat gggcaaggcc acggcgaagg cgtgcgctcc cgtcggcgcg
     1141 gtgctgacga tctccgccgc gggcagcgag acgagcaact cctgcgtcat caccaacgag
     1201 gacggctggc tcaaaaaggg tgtcaactac gaatgctccc gcccgcgctt tgccattatg
     1261 aaccccgcgc tcacctgcac gctccccccg tatcaaaccg ccagcggcgg cgtggacatt
     1321 atgatgcaca cgatggagcg ctatttctcc aacaccgagc acaccgagct tctcgaccgc
     1381 atgagcgagg gactgctcaa aaccgtaatg gcgaacacca aaaaggcgct cgccgagcca
     1441 aacgactacg acgcgcgcgc cgagctcatg tgggcgggca gcctttccca caacaatctt
     1501 atgggcgtcg gcaaggtgca ggactgggcg acgcacaaga tcgagcacga gctcggcggc
     1561 atgttcgatg tggcgcacgg cgccggtctc gccgccgtat ggggcagctg ggcgcggtat
     1621 gtgatgcacg ccaatctccc ccgcttcacg cgctttgccg tggaggtcat gggctgcgag
     1681 atgaattacg cgaaccccga ggagaccgct ctcgcgggcg tcgccgcgtt cgagagcttc
     1741 tgccgctcgg tcggcatgcc gtcgaatctg cacgagctcg gccttggcga tattactgag
     1801 gcacagatgc acgaaatggc cgtgaaatgc acgcagaacg acacgctcag gatcggcggt
     1861 cttgtccggc tgtacgccgc ggatatcgaa aagatctacg ccgccgcgaa ataaaaatcc
     1921 gaatcattaa gacacgccgg tgcaggtacg catacttgac accggcgtgt cctttgtgaa
     1981 taatagaatc accgtcaacg gcgaaaggag gcgcggcgtg agcacgaagc ggatcacggt
     2041 gtattactcg ctcaatcaag tgggattctg gatggtcttc tgcctttcca gcagctttgc
     2101 cgccgtttat ctcggcgctc tcggctacag caacacaaag ctcggcagca tgctcgcgct
     2161 gggcagcgtc ctcggctttc tgctcggcac agggctctcc tcctttgtgg acacctcccc
     2221 gcgcttcaac gcggcggatg tgctctggcc ggtgctgacg cttcaggcgg ttttcttcct
     2281 ccttctgctg tttttcccgg cgcgcggcgt cgttacgacg ctgtgctatc cgctgctgct
     2341 ggcgttcact ctcgcggtga acacgctcaa tctgaagctc tgcgccgatt ttgagcatta
     2401 cgaatccccc gtcaattacg gcattgcccg cagcatgggg tcggcggcgt atatggtgct
     2461 ttccatgctg ctcggcgttt gggtcacgcg ctttggcgtg cgcgtcatcc ccattgccgg
     2521 gcttgccgtc accgcgctgc agggcgtttc caacgcgctg ctctcgcgct ccgtcctccg
     2581 gctgcgcagg aagctgctct ccggccatgc cgcaccggcg ccgcagggcc gttcgctcgg
     2641 cgtatttctg cgggagagtc cccgcttctg tctgctgctc gccggaatgg cgctcctctt
     2701 tttctcgcac aacaccacgt ccacgtttct catcaacttt gtggagcacg ccggcggcag
     2761 taccgagacg ctcggctatc tcaccgcgtt cctcaccgcg ctcgaggtgc cgacgatgct
     2821 gttcttctcg cgcattttcc gccggaggag ctgcgccgcc gtgctgcggt tttcgtttat
     2881 cttcttcgcg ctcaagaccg ccgcgatcgc cgcggcgccg aacatcccgc tgctctttgc
     2941 cgcagagtct cttcaggtgc tgtcctatgc gctgtacgcg cccgcgatcg tggcctatgt
     3001 cacgctcatc gtcccctacg acgacgccgc caagggacag agccttgcct tcagcatgac
     3061 gaccataggc ggcgtgctcg caagtctcat ctccgggcgt ctgctcgatg ttctttccgc
     3121 tccggcaacg gcatggatcg cggcggcggt ctgcgccgtc ggcgcagcca tcgcttgccg
     3181 cgggctggaa aagccgaaga atatctctgc tgtctggcga ccgtaaacaa ggacaccaaa
     3241 agatttttcc aaacggctgc ctgcgatggc ctggacgatc ttgtcaatga gcttccccct
     3301 tcggataccg gccatcgacc atggaggaat tgaccgacgc cgtgatgaat tccacgtatc
     3361 tgtatgaatc ctccccacgg ttttagaatg ggcggccagt tgtctgcaaa aaaagcagcg
     3421 gaccgaaaaa ccaacgaaaa cctcggacga aaaaaggcga acgaaacttc tcttttgaca
     3481 ttcctgtccg agacgaacaa cctccccgaa tgcagctgca atcggggagg ttgttctata
     3541 gggtatcgtt taagttttag aggataggcg tccaggcgag gttcagaatc tcgtccgtat
     3601 cgtccatgga ggtgatgagg ccgaggcgga cgtagcgctc gataaagtcc ttgagcgtcg
     3661 cgccggtgaa ttcgtcggag aggccgaact gcagcgcatt gttgatcatg atgttcatct
     3721 cgtagttgcc gccgttccag cccatgtcca tgagcacctg ggtggcttcc gcggagttat
     3781 cgcgcatcca gctgtgggcc ttctgcacgg cctgcgccac cttcttggcg atggtcgggt
     3841 tttccttcac gaaggtgccg ttcatagcca gaacgcagca ggtgcggtcc gcaaagtcgg
     3901 tatcctgctg ggagcggatg cacttgagct tgccatcctt caccatggca taggcaaagg
     3961 tatcgctcag cagcgccgcg gcgatctcgc cgttttccat agcggcgacg caggcgtcag
     4021 cggtgacctt cacgagctcc acgtcggtgg catagttgat gccgtcagcg tcaagcagca
     4081 gagcggtgat gttatagtcg gacatgccga tgccgttcgg agtggagatc ttctggccct
     4141 tcaggtcggc ggtagtgttg tattcgctgt ccgcgagaac gtacagagac ttgcagccga
     4201 tatgggagcc gccgacgaac gtgatgtcca cgccgttggt gatgggaacg agcatcttcg
     4261 ccatcatgcc ggaggcaaca tccaccttgc cggtgccgag gccttccacg tccgtggtgc
     4321 ccttaacgcc ctcggccgtc agaccggcct cggcataata accgagatga tcagccatgg
     4381 tcgggccgga ggtgcagccg tcggacatga ggtacacaat gggacggcca taagccggct
     4441 cattcttcat ggcttcgagc tcttctgcgg tcggggtgag atcgaaatcg gtcatttcga
     4501 tcgcgcggcc ttcgatctgt acggggacga acagcgcttc ccagttgccg gaagctgcgg
     4561 cctcggcagc gtccatgtgt tcgctggctt cgttgacttc cacatcttcc acggtgccga
     4621 tctcgatctg gccgtcgttg ttctcgtcgg cgatcagggt agaggaaccg gtatttccgg
     4681 tcccggcgtt gtcgctggtt tcgggggcct gtgcgccgca cgcgcagagc gcgaacacca
     4741 tgacgagcgc gagcacaagg gcaagggtct ttttcatgta cttttctcct tcaatttttt
     4801 atttacaggg cattgccata taaattctta ttcagtcttt tttccgccga agtgcagacg
     4861 gttgagaatt tcgttgcggt actcgacgaa gctcgcagac gaacggtcgc gcgggaagtc
     4921 ctcgtcgatt cggatatcgg caacgatgcg gccggggttg gactccatca cgagcacacg
     4981 cgtgcccata tacacggcct cgtcaatgct gtgcgtcacc atgagtgcaa gctgctttct
     5041 ctgcgaccag atgttcaata tctcgtcctg catattcatg cgcgtgaatt cgtccagtgc
     5101 gccgagaggc tcatcgagca gcaatatctc cggttcgttg atgagcgtgc gcacgagcgc
     5161 cacgcgctgt gccataccgc cggagagctg tgcgggatag tcgttccgaa accgttccag
     5221 accgatcatg cggatcattt gatccactcg ctcggctttc ccgtctttca tgccgcgcac
     5281 atccagactg aacgcaatgt tcttttccac cgtgagccac gggaaaagcg taggattctg
     5341 aaacacgacg ccgcgttccg gcgaaggtgt ctccacctcc gcgccgtcta ccgtgacacg
     5401 gccgagtgtc ggcaggatga ggcccgccac aaggcggagg atcgtggact ttccgcagcc
     5461 ggaagggccg acaagggaga caaactcccc gcttttcatc gtgacgctca catcctgcag
     5521 cgcgtgcgtc acctcgtcgc tttctacctt ggcaaaaacc ttgctgacgt tttccagcgt
     5581 caggcagaca tctttctttt cgtttcgcat tacttttccg ctccgttctg ccatctgagc
     5641 agatagcgtt tgatccgttc gagccccttg gtgaccagcg tgaagatgaa gcagatcaca
     5701 aagagcgccg cgaaggattt atcgtagctc gcccagccgg tctgccatgt catataccaa
     5761 ccgaggccgg actttacgcc gagcatttcc gcaatcatga tcacgataca cgaagaactc
     5821 atcgcctgcg tgcagccctg cagaatggac ggcatggcgt gcgggatcgc cacgcggaac
     5881 acgagctgac ggtcgttcgc gccgagagtg cgcgccgctt caaaatactc cttgcccacg
     5941 ttggcgatgc cggtgagcga ggctaccgtc acagcgaacc aggaactgag tgcaatgatg
     6001 aacaccgcac cgccgaaaag tgaggtagca atgaccatga tgatcggaat ccacgtggat
     6061 gtggggatcg gccccaaaaa cttgatgatt gggtcgatcc agtagcggat gcgcttggaa
     6121 tagccgcatg caatacccgt cacaaggccg agggatacac cgataaagta gccgagaaac
     6181 agcagcttga gcgtgttgag cgtacagtca gccagcattt tgtaatcggc aagaaacgca
     6241 ttgatgatat agttcatgca cggcacaaac ggctgcgtca gcacgccggt cttcagcgtg
     6301 agataatcgt acagcgtcag gaacagcagc agcaccgaat agcgcggcgc tctatagtgc
     6361 agcgactcat aaaagtcttt cttcccgtgc tgttttccga tcaccgctgc gagcaggcag
     6421 acgatataca ccgccagcgc cgcgccgagc acccagacat agctccacgg gttccggttg
     6481 cggctgttgt tcggcacgag gagatattca agcagcgcga taccgccgca gagcagcggc
     6541 aataccatga tgaccgcctc cgccgttttc tgggcgggtg tcttttcctt ttcctccagc
     6601 tccctgagct ggcggcgggt cagacctgag agcagcgcaa gatcggtgct ctgtnnnnnn
     6661 nnnnttttcg ctgctctcgt cgctttcttc ggttttttca attaccgtgg caaaagagcg
     6721 aaaggcgtgt tatagtaggc gcgagaaaaa cgaggggaga atcacggcat ggactacgcc
     6781 atcggcatcg atatcggctc cacctgcgcg aagacggcgg tactcgccgc cgacgggtct
     6841 ttcgccctgc tctcggcgcg gccgacgggc tggagcagtg tggacacggc ggagagcatc
     6901 cgcgccgagc tcgagacggc gcacttttcg ccggaaacgt acccctgtgt tgcgacaggg
     6961 tacgggcgcg tcgccgtgcc gtacgccgga aagagcgtca cggagatcac ctgccacgcg
     7021 cgcggcgcgt gcttcctgca cgggcgggac gatcttctcg tgatcgacat cggcgggcag
     7081 gatacgaagg tcatcggcgt atccggcggc gcggtgcagg actttcttat gaacgacaag
     7141 tgctccgccg gaacggggcg ttttctcgag atcatggcaa accggctcgg catgcgcccc
     7201 gaggagctgt gcgcgctcgc cgcgcgcggc ggaggcgcgt ccatcagctc gatctgcacg
     7261 gtgttcgccg aaagcgaggt cgtaagtctc atcggtcagg gtgcgccgcg cgaggatatc
     7321 gcttacgcgg tcgtggattc tattgccgga aaggttgtca cacaggcaaa aaagctcggc
     7381 gggagctatg gcgcggtatg tctcaccggc gggctctgcc agacggaata cctgcccgac
     7441 gccattgccg aaaagctcgg cacgccggtc ctcacgcatc cgcaggggcg ctttgccggg
     7501 gccatcggcg cggcgctcgc cgcgcgcagg ctttcccgat aacaaagaaa cgaggtcata
     7561 tatgcatgga gctctctctt cccgaaacat ttgaagccta ttccgacgcg cgcaaagcgg
     7621 gctttctcaa ggtaaagacc gccaaagaag gcgggcggcg cgtggccggt acgttctgca
     7681 cgtttacgcc gctggagatc ctggacgccg ccgggatgct cgccgtgagt ctctgcggca
     7741 tgagcgccga gacgatccct gccgcggaag tcgacctgcc gcgcaatctc tgcccgctca
     7801 tcaaatcgag ctacggcttc tatgtgagcg acaagtgtcc gtatacctac ttttccgatc
     7861 tcattgtcgg cgaaacgacg tgcgacggga agaaaaagat gtatgagctg atggcgcgcg
     7921 gcaaggagct tttcctgctg catctgccgc agggcgttga tctgccctat gcgcgcgata
     7981 tgtggcgcgc cgaggtgcgg cggctgaagg aatatcttga agagcgcttc ggcgcggaga
     8041 tcacggacga tgcgctgcgc gccgcggcgc ggcagagaaa cgcgctgcgc gaggcccgct
     8101 cccatctcat ggaagtgctg cgcgccgacc ccgcgcccgt ttccggcacg gagctttata
     8161 aggttctcga cgggctgggg tttgattttg acctccccca cgctgtttcg catgtggagg
     8221 agcttgccgc gcgtctgcgg gaaacaaagc cctccgcccc ggcaggggga ccgcggcttc
     8281 tcgtcaccgg ctgcccgatc ggcggcgtat accagaaggt gctcggcgcg gcggagcgcg
     8341 cgggcgcgca ggtcgtctgc tttgaaaact gcgcgggcat caaaccggcg cgctgccacg
     8401 tagacgccgg ggcgaaggat attctgagcg ccattgccga cgcctatctc accatcggct
     8461 gcgccgtgat gacgccgaac cgccgccggt tcgatatggt cccggcgctc atcgaagagt
     8521 tccacgccga tgccgtgctc gatatcgccc tttcgagctg ccacacctat ctcatcgaag
     8581 gccgttctct ccgcgagctc tgccgcagtt tgggcgtgcc gtatatggcg ctggagaccg
     8641 attactccca gctcgacgcc ggacagatcg acacgcgcat cgccgcgttc gtggaatcgc
     8701 tgtaaatacg gaaaaaggct cccttcacgt ttaaaaaaac gtgaagggag cctttattgt
     8761 ttttcggatc attccgttgt ttttaccggc tggacaatgt ccgtgagatt gcgcacccag
     8821 cgctcgcgct tgagcatgaa gtgcgcgatg gtgattttca cgaagtccac ggccttgacc
     8881 atgccgtaca tggccaccgg gccgatcgtc gtatagcgcg ctacaaggaa gatgccgggg
     8941 atgatcataa gaagcgtcgt tgtggcgtcc acccataggc ccatggcggt atcgcctccg
     9001 gcgcgggaca cggcaagctg gacattgata tacacccacg gcggcataaa gagcgacatg
     9061 agaacgatca tccgccgcgt gatgcttcgg gcgcttgcgc tcaaatggcc gaacaccacc
     9121 gggatgagcg gaacggtgag caatccgacg aagatcataa aaaatccgaa cacaacgccc
     9181 gccgtcataa gccagcgctt ttcctgccgg gctttgtcca gttcaccgct gccgagcgta
     9241 gagccgagga taacgccggt cgcggtcgtg atgccgctga acgctacgaa aaagagattg
     9301 gcaatggcga agctcgccgc cataccggag acgacgtccg cgccgccgcg gccattgtaa
     9361 agcgcggtcg tgaccgtttc ggaaatgacc cagagcattt ccgagccaag caccatcgag
     9421 ccctttttga gcatgcggaa gaagagcggg aaatctacgc tcacaagctc tttcggcgta
     9481 gcggaaaacg gcgggcgctt gacggcaatg aaaacgagaa acattgtcat ttccgtgaga
     9541 cgcgcgatga tcgtggcgat ggccgcgccc tgtacctcga gccgcggcgc gccgagattg
     9601 ccgtagatca gcacccagtt gaaaaacgtg ttgacgagcg tggcaataac ggagatgtaa
     9661 agcggcgtac gcacccggcc gatctcgcgc agggacgacg agcagatcgt ggaaatggcc
     9721 atgggaatac cgttgaagcc catgaggaac atgtacttca cggcctcgtc caatatggcg
     9781 tccgcctgcg annnnnnnnn nccatgagcg agagtacctg ccgcgggaac accatgcaca
     9841 caagaagata gagcacgatg gccaccgccg cgagcaccgc tttgaagctc agcgcctgac
     9901 gcataccgcg cttatcgccc gcgccgtaat actgcgacat gaagatgccg cccgcggtgc
     9961 agatcgtatt gagcagcacc atgaatacga aaaggatctg gccggagata ttgacgccgg
    10021 acattttcac gtcgccgaga cccgagacca tgaagttgtc gatgagcgac accatgttct
    10081 ggatgagcat ctgcagcatg accggcagcg cgatgagcag cgccttttta taaaagctcg
    10141 ccgggccgaa aattttgccc tttctgagcg ttgcagtttc catgaaacct tgcttcctcc
    10201 tgataaatca tctgaaagtc agatgattct atccacttga cgcggaaaag tcaataggtc
    10261 aaatttcacc attcactgcg cgcttttttc cacgatttgc agcacctcgg cgatatccgg
    10321 gatcgtgtag tcgatgcgga gctccggcgg cgcagcgtgg ccgtgggggt tataccagca
    10381 agtctgtatt ccggcgttga cgccgccgcg gatatcactc gtgagcgagt cgccgacgat
    10441 cagcatctcc tccggcttta tgccgggaaa cgccgcgaac accggggcaa agaacgccgg
    10501 cgacggcttt tccgccccca cgacctcaga gatgaagatc gggtcgagca gcttatcaag
    10561 ccccgagcgc tcgagctttt tgtcctgcgc gatcttcgtg ccgtttgtca cggcgcactg
    10621 cgcagctatg ccgcggaggg cctgcacggt ttcgagcgcg tgcgggcaga atttgatcgt
    10681 atcgccgaga cggagctggt attccgcgtt gaacgccggg gcaacggcag tatcgatgcc
    10741 gcgcgcggtg aaaaactccg caaagcgccc taccaggatc tccggcttcg tatactcgcc
    10801 gcgctcgagc gcctgccacc agcggacgtt gatggcggaa tactgcgcga tcatctcatc
    10861 ggtacactcg ccgagaccga agagagaaaa gcaagtcttt attgcctcgc gctcggcggc
    10921 gagaaagtcc agaagcgttc cgtccacgtc ccagaggaca gctttgatat cactcatggc
    10981 gcgcctccca ctgttcccgc agccacgcga tctccgcggc gtacccggca tcgtcattcg
    11041 gcgtttcgag aatgaacggc cgtccggcaa gggctttgtg gcagacgatg cgcaccagcg
    11101 cctccggcgg cagaacgccc tcaagaattt tggcatggcg gtccttatgg ctgccgagac
    11161 cgttgaggga gttgttaaga tgtaccgcct tgagccgatc aagaccgatc acgcggtcaa
    11221 actcggcaaa caccgcgtcc gggtcggaga cgatatcgta gccgccgtcc cagatgtggc
    11281 atgtgtcgag acagacgccg agcttgtctt taagctccac gcggtcgatg atctcgcgca
    11341 gctcctcaaa ctttccaccg atctcgctgc cctttccggc catcgtctcc agaagcaccg
    11401 ttgtgtgcat gtctggcgag agaaccgcgt ttaaaagctc cgcgatcatt tcgatcccgg
    11461 cctccgtccc ctgcccgacg tggnnnnnnn nnnacgtggc tgccgggatg gaaattataa
    11521 aagcagccgg gcgtgagctc catgcgttcc agatcttccg taaaggcgcg tttggcaaag
    11581 tcgcgcaccg agggcttgtc gctacacggg ttgagcgtat agggcgagtg cgccacaagc
    11641 gggccgaagc cctgttcggc cgcccacgcg cggaacgccg cgacgtccgc ttcctccacc
    11701 ttggccgccg cgccgccgcg cggattgcgc gtaaagaagg caaacgtgtt cgcgccgagc
    11761 gccgcggcgc gcttccccat cgccatatac cctccggcag aggatacatg gtagccgaga
    11821 tacagtgtat tagttgacat aatattctcc tgctgcgttt ttttcagtat agcacagttt
    11881 ccgaaatagg aaaagcccgc ggtattttcg ccgcggactt ttcctatgca gagagcacat
    11941 aaacaaaaga ggcaatcgaa tccgcgtagc actcctgccg aaaacccagc ttttcaaaaa
    12001 ggcacaggct tttccgattg tctttgtcga tcagcgccac aatatccagt gtcggatact
    12061 tttcccggac ggaacggatc acttcccgca gcatttcgca ggcatatcca tttctctgga
    12121 aacgaggggc aacggtgata cccagcgtga tacagtcccc cggtttataa aagtacgcca
    12181 gttcacctac aagctccaag ctgtttcttt gggcgatccc aaagtgctgt tcctgcttgt
    12241 ctgacaggaa aacatcgctt tgaaacttct caatatacgc ctcgatatcc ggaagcgtga
    12301 aggcctccca ccgctgatac cgtcggcatg cctcgtcgtt tcggtatccg ctgatgcttt
    12361 ccgcatcgga tagctccaga ttccgaagag acagcctctc cgtttctaaa taaatcgtac
    12421 gttttctcct tctaaataaa tcatacgttt tctccgtgtt cgccgggaga ttttatcttg
    12481 tcgggacgat ctccagccag tagccgtccg ggtcctgaat gaagtagatg cccatggcgg
    12541 cgttttcaaa gcagatgcag cccatttcgc tgtggagcgc gtgcgccgcg gcgaaatcgt
    12601 ccgcacggaa cgcgagatgg aattcttcct cgccgatgtc gtaggcctgc ggatggtcgc
    12661 gcagccacgt gagctcgagt tcaaagtcgg aagcgtcgtt gccgacatac acgatgatat
    12721 acgagccgtc agcggcttcc ttgcgccgct tttcgtgcag gccgagcgct ttctcgtaaa
    12781 acgcaagtga ggcggcaagg tcgcagacgt tatagttttc atggatcgtt ttgaactgca
    12841 tggttttttt cctttcagat cgtgaatgtc tggttgggcg cggctatatc ggcgatccta
    12901 tcgcggtagg cttccgttac gctcatcgcc ttgaatttgg ggatatactc atacttttcc
    12961 cacatgtgca tcgggaacgc cttgcggatg tccgccgagc gcatcatggc gtcaaagccc
    13021 atccagtagt ttgcgccgag gcgcgggtcg agcgggagaa acgccgcgtc cagccggacg
    13081 ccgttgagct tggcgatctc ggcaaagtag cggtttttca tatcgcgctc ctcctccggc
    13141 gtatcgtccg gccacgccca ccagtggaga tcgcccgcgt ggtatacggt ctttccggcg
    13201 tagtttacaa cgaacgcaac gccgcagtcg gtcgagccga cggtggaaac gctcatcgcg
    13261 cccattttcc cgcccggaaa gatgcggcgg cagtttggcc ctgtacggac aaccggggcc
    13321 ttttccccgg caggaacggt gcgtatatcg tccgagagaa tgaacgtgtg gcgcgggtgg
    13381 tcggcaaagc gggtaaatat ctccgggttg aagtggtcgg aatgatggtg gctcgcgaaa
    13441 acgcacaggc gcttatcgcg gtctatctcc ggcagctcgc cctcggcata gtcaaaaaga
    13501 cagacaatat cctcccattc cgcaagaaac gcgctgtgcc ggatgtatgt aatgcgcatg
    13561 gtgcgcctcc ttcatgattt acataagtga aaaaagtgtt ttttatgtaa atcaagtata
    13621 cgggccgcag ggcgctcggg caagttacag ttttgacttt tttctgtcag tttctgccaa
    13681 cgtcggcagc gagcattgaa agcgcaatgc gctttttcgc cgcgtccacc tcgacgacgg
    13741 caacatccac cacgtcgccc acgcggacga cgtccgaggg ctttcgcaca aagttgtccg
    13801 aaaggcggga tatatgcaca aggccgtcct gatgcacgcc gatatcgacg aacgcgccga
    13861 agtccacaac attgcgcacc gtgccgcgca gcttcatgcc gggcttcaga tccttgagct
    13921 ccagaatatc ggtgcgcagc agcggcgcgg gcagctcgtc gcgcgggtcg cgtccgggct
    13981 tcatcagatc ggatacgatg tccgagagcg tcggcacgcc gacgccgcag tgcgccgccg
    14041 ccctttcctc accgagcgcc tttacgcgct cacgcagctc tccgacgcgg tgggcgcgca
    14101 catccgcctc cgtatagccg cacagggcga gcagcttttc cgccgcggcg taagactccg
    14161 gatggacgcc ggtcgcgtcg agaacgtttt tcccctgcgt cacgcgcagg aagcccgcgc
    14221 actgctcaaa cgccttcggc ccgatgcgcg gcaccttttt gagcgcggcg cgcgacgggt
    14281 acgccccgtt tgcctcgcgg taggcgacaa tatttttcgc cgccgtgtcg ttgagcccgg
    14341 cgacatagga aaggagcggc gccgacgccg tgttcagatc ggcgccgacg gagtttacgc
    14401 agtcctccac cacgccgcgg agcgtttcgt ccagccgcgc ggggggcata tcgtgctggt
    14461 actgcccgac gccgatgctc ttcggctcaa tcttcacgag ctcggcaagc ggatcctgca
    14521 agcgccgcgc gatggatacc gcggagcgga gcgaaacatc gtaatccgga aattcctccg
    14581 cgccgagctt ggacgccgaa tagacgctcg cccccgcctc gtttacgacc atgtaggcca
    14641 cgccgggaag gtcgcggatc atatcggcga taaatatctc cgactccttg gacgccgttc
    14701 cgttgccgat ggcgatggcg gtaacgccgt ggcggcggat catggcggtg agagttttgc
    14761 gcgcctcgtc cttccggttg tgcggcggcg tggggtacac aacgccggtt tccagcacct
    14821 tgcccgtctc gtccacgacg gcgatcttgc agccggtgcg gtatgccggg tcaaagccca
    14881 tcgtgacgcg gttccggagc ggcggctgca tcagcagcgg ctggagattc tgtccgaagg
    14941 cgcggatggc tgcctcgtcc gccgcgtccg tcagctcgcc gcggatttcc cgctccgcgg
    15001 acggctcaat gagccgccgc caagcgtctt cacagcaggc atttacgaaa gcatttgaga
    15061 tgctgatgcc gcggaacacc gcccggcgga tctcctgcag ggcgagcgcg tcctcgatct
    15121 ccacgccggc cttgagcatt ccctcccgct cgccgcggtt caccgcgaga acgcggtggc
    15181 cctgcaccct ggacaccggc tcgcggtagt catagtacag gcggtacacg ctgtcctcgt
    15241 ccgttgcggc ctttgcccag agcacgccgc gcgtgcggta cagccgccgg agcgcggcgc
    15301 ggatggcggc atcgtccgag atcgtttcgg cgatgatatc gcccgcaccg gcgagcgcgt
    15361 catcagttga cataactcct ttttcttcgt tcacatacgg cgcggccagc gcttcgagtg
    15421 tgccggatgt gaccgtctgc gagagcagca gctccgcgag cggggcaagt cccttttcac
    15481 gcgcgatcgt ggcgcgcgtg cggcgcttct gcttgtaggg gcggtaaata tcctcgacct
    15541 cggcgagcgt ctgcgcgttt tcgatggcgg cagagagctc gtccgtgagc ttgccctgcg
    15601 cttcaatggc gctcacgacg gcggccttgc gctcgtcgag accgcgcaga tacgcgagcc
    15661 tgtccgcgat ggcgcggatg gtctgatcgt cggtcgtgcc gtgcatctcc ttgcggtagc
    15721 gcgcgatgaa cgggacggtg ttgccctcgt cgagcagacg gaggatgttc tctatatgct
    15781 cctcccggcg ggaaaactcc ttcgcaagaa ggggggtgaa tttatccatg cgtggtgtct
    15841 ccttcaaaag attggatatg aattttacca caattccccg cgccgggcaa ggagcattgt
    15901 ttcctgttga caagtttact tggcattgct ataataacaa caagaaaact tggcatagga
    15961 ggatcgatat ggaaaaggaa gaaattctgg cgatcagccg ccgggaaaac aagaaccggg
    16021 atcttgcgga agcggcaacg gcacagcagg ccgggaatat tgccgggcgt gtcggcgcat
    16081 gtgtgtgctg tctggtatcc gtggtcttcg tctgggcgac cggaacgatg ctctacagtc
    16141 cctgggtcat ttatttcagc attctcggca cgcacagtct ggtgacatac cgaaaaaaga
    16201 aaaggaaaac ggagcttacg ctcactattt tgtattttgc catgtttctc ctgttccttg
    16261 ttcttttcgc tgtacggctc gttggggtgc gcggatgaac gacgagctga aactccgaaa
    16321 ccgcctgaag gaggctcgta cggaaaaggc gctttctcag gcgcagcttg ccgagatggt
    16381 aggcgtatca cgaaatacga tcagctccat tgaaacgggc caattcaatc ccacggcaaa
    16441 gcttgcctta gtcctctgca tcgctctgga taagaaattt gaagagctgt tttattttta
    16501 aggaggccgt catggaaaac tatctgctgc tcattgtggg cattcttttg tccgttctcg
    16561 gtgcggtgaa tataaagggg aacatccgca cgatccacgc ctacaaccgc cggaacgtca
    16621 gagaggagga catcccgaaa tacggccgcg ccgtcggcac gggaacgctc atcatcggcg
    16681 tttcgctcgc cgcggcgttt tttgtttccc tgcggaatga ggctgccgtt tcgttcatcc
    16741 ttcttccggg gctcgccgtc ggtctggcat tcattctgta cggacagatc aagtacaatc
    16801 acgggatttt ttgattcttt ccattggaag cgaaagagga cggtgctccc gtcctctttc
    16861 gctttgttcg ctttaaaaaa atgacgttta gcggaaaacc gcatattgat acgtgggtgc
    16921 agccgctttt ttatacgtaa tcagctcaaa cagaacaaga tcataatttg atgcctcatc
    16981 cagatccaat acatcattca tggcaaaggc agcttcgtct tgggttggcg aaaaattata
    17041 tagcgtggca tagggggaag tcaggatcgt tccgtcgttt tccttggcca agcgaagata
    17101 aagctgaata tcatgttccg gcctgcctcc cttgcacgac agactcagcc ggttcacctc
    17161 tggcagcaga ggactctctg tcatagaaga taacgcttcc tccggagtct catacatgag
    17221 tatcggttga tcgtttactt gcaggcggtg tgtattgcaa tggggcccac tctgcacaga
    17281 aaaactcacg atgccaagga cgagaaaaaa agcaagcatc acccatatcc gtttccgcat
    17341 ccgatccacg ctccttcctt taaaattcaa atattgaaca gcatatttat ataattcaag
    17401 aatatacggc gttcacaaca ttgtcaatac cgcgcatcca agatttggaa atcttcttgt
    17461 cttgactttt tccggcgagg cggatagtat gagagcatga tgaaacgaaa gatatttttc
    17521 cttctcccgg cgctgctctg cgcggcgctc ctcgccgggt gcagcggcag cgcgctcgac
    17581 atctttttcc cgacaccgtc gcccacggcg accgcggaac cgacgccctc acccaccgcg
    17641 gagccgacgc cgacacccga gccgacgccg gagccgctcg ttccctatga gggcgaaata
    17701 cggcacatct ttttccacag tctgatcgtc tatcccgaaa tggtgttcac cgaccgcgaa
    17761 acgccgatgg gcggctacaa cgccgggttt tccgaaaagg cggagctcga aaagatcctg
    17821 ccgcagctct acgagcgcgg gtatgtgctc tatgatctga acgagtgcta cgaaatggca
    17881 gacggcagaa tgcaacgaaa ggagattttg ctgcccgccg gaaaaatgcc gctcatcctc
    17941 tcggtggacg atgtggcgta tgcctacggc gacgggtacg cgcggcggct tttcgtcgat
    18001 gatgccggtg cgcttctcta cgaggtgccg aacccctccg gcggcgtgga tatcctctca
    18061 gacggcgatg tgatgggcgt tgtggacgct tttgttgccg agcacccgga cttctcgtgg
    18121 aacgggcaca agggaacgat cgcgctcacc gggtttcagg gagcgttcgg ctacccgagt
    18181 tatgacgatc ccgcacacca gaccgagatc aaaaaagtcg ccgacgcgct gcgcgccgac
    18241 ggctggagct ttgtcagaca cagctatacg cacaactccg gcgtgtacgg cttctggggc
    18301 acgggctgca tccccgataa aatttattac gacatcaaca agtgggtcga tcgcgtgtcg
    18361 ccgtggatcg gtgaaacgaa cctctttctc gcccccttcg gctaccgcgc gacgggcgaa
    18421 gcattgcagc atgtgctcaa tgccgggttc aatatctact gcaccgtgga cgaccatgtg
    18481 gtcaacgagc tgtacgataa ttacgcgctt cagtcgcgca ttgagatcgg cggttattcg
    18541 atgacgtatt accgcgatat tttaaacgag ctctttttcg acgtggacag cgttctcgac
    18601 cccgaccgcc cgccggtcgt atacgatgaa tgaaaaaccg ttctctgcgc ttcatgcaga
    18661 gaacggtttt tcttattttt ccttgcgcgt gatcgcgccg aggagcttat ggagcggggt
    18721 cacggcgcgg tagtaccggc ggtagacctc gcggtagatg cgatcataga gcttatgctc
    18781 ctccgcgtcc ggcgtgaacg tgtcgcggat ccgcaccatg tgggcgatgg cgtcctcata
    18841 ggaggtaaat ttcccgagcg agacgaacga gcagatggcc gcgccgagcg cgcaggattc
    18901 gtgcgtctgg atgcgggaga ccggaaggcc gaacatattc gccgtgatct ggcagatctc
    18961 gtcgctgcgg ctgccgccgc cgccgagata gagatggcgg attttctgcc ccgagcggcg
    19021 ctccatgccg tacatgccgt ccatgagggc aaagttcagg ccctcgatga tggcgcggta
    19081 catgtggtat ttcgtgtggt ggtcggtaaa gccgatcatc gcgccgcgcg tgttcggcgt
    19141 ggagaggccc ggcgtccagt gcggcagcac gagcagaccg tcgctcccgg cggggatatc
    19201 gccgaggtgc ttgttcaata tttcctccgc cgagcagccg agcgccttgg ccgcctcgac
    19261 ctccgcctgg gcgaagttct gcttgaacca ggtgagcatc cagtagccgc gcaggatttg
    19321 aatttccggg ttatacatgt cgttcggcac cgccgggtac gcgggcatga acggcgtcgg
    19381 ctcaaagtac tttccggtcg tgaactgcac ggtgcagctc gtgccgaacg agagcgccgc
    19441 ctggtcctcg cggatgacgg aaaggccgat cgtctcgcag cccttgtccg agccggtggc
    19501 gagcatcggc agccccgccg ggatgccggt cagccgcgac gcctccggcg tcacgccgcc
    19561 gacgaccgag ccggagggca agatctcgca gagcttatcc tgcgggatat cgtacacgca
    19621 gcgggtgaga ccgtttttgt cccacttgcg gtttttgtag tcatagggga tcttcgccat
    19681 gaggctcgcg ctgctgtcgg caatgcggcc ggtgaagcga tagttgagat acgtggacag
    19741 gcagacgtac ttatccgttt tcttccaaag ctccggctcg ttctcggcga tccagttgca
    19801 ggccgaggtg cggtggagca tttcaacggt ctccgtcata ccggcgagcg cgaagatcgc
    19861 cctgcgttct gccgggagcg gcttcgggtt ttcggcgcgg cgctgatcca tccagaggat
    19921 gcagtcgcgc agcggccgcc cgtccttgcc catgcagacg gtcgtatcgc ggaacaccgt
    19981 caccgcgacg ctcgcggcgc gcgcaaaatc ttccggggac tgatccttca gccgctggga
    20041 gacgatgcat atatgctcaa aatagaaatc gggccgctgc tccgcccagt tcggattgcg
    20101 ggaataatag ggctgctcat aaatgtcctg cgccgagcgg acaatgtgtc cggcagcatc
    20161 gaagagcatt gcccgcacgc tctgcgttcc gacatcaaat accagcgcca atgcatcgtt
    20221 catagcctta tgcctcattt ccgtgcagtt ttttcttgtt cggatggaag agccgccaga
    20281 gccagccgtg tttcttctcc ggcgggagcg gttccacggt cttttctccg aagtactcat
    20341 agagcgtgtc gtggctcgcc gtgccgggga cggtctcggc gcgggaatag ctcccgtccg
    20401 gctgcatcgt ccagctcttc tcccggtcgg cacgcagcgc gtcgagaatg gcgagcacgc
    20461 cctcgcgcgt ttcgggcgtg acgcattcga tgaatgcctc aacacggcgg cgggtgttgc
    20521 ggttgagaag atcgccggag ccgatgaaaa tacgctgctc cgcaccctcg ccgaaggcga
    20581 agatgcgcga atgctcaaga taccgcccca cgacgctgcg caccgtgata ttctccgtat
    20641 accccgcgac gcccggccgc aggcagcaga tgccgcggat gaaaagctcc gtacgcacgc
    20701 ccgcgcgcga ggcgcggatg agcgcagcca taacgccgat gtcgttcatg gagttgacct
    20761 tgatcgcaat gcgcccggcc tcacccatcg cggtctcggc ctcaatgagt tccagcacac
    20821 gggagcggta gctcagcggg gcgacccaga gcgcttccgt ctccggcggc gtctggccgg
    20881 tggcgagcgc ttcaaacgtc tgcgcggcgt cgcgcccgac gcgctcgtcg gacgtaatga
    20941 ccgaaagatc ggtgtactgc tcgctcgtga cctcgttata gtttccggtg ccgacctgcg
    21001 tgaagtactg cacgctctcg ccgcgctttc gcgtgatgag gcagagcttg ctgtgcacct
    21061 tcatctccgg caggccgtaa atgacctgac atccggcgtc ttcgagcacc tcggaatagt
    21121 caatattatt ctgctcgtca aaccgggcgc gcagctccag aaggcagagc acgctctttc
    21181 ccctgtcggc ggcatacgcg agcgctgcgg caaccttgct gcttgccgag agccggtaga
    21241 gcgtgatgcg gatggaaacg acgtccggat cgtccgccgc ctcgtagaga tattccacga
    21301 acgcgctcat gctctgaaac gggtacgaca gcaacagatc cttcttttcc agatacggga
    21361 aaaactcgcc cttttggagc ttcaccgttt tctggctgcg gcgctcggcg tatttcatgc
    21421 cgggcgcttt cttcacgccg gagccgaagg agaggtcgaa cggcagcttc gtggtgaaca
    21481 cgttgctctc cgggaccttg agatccctga tgagcgccgc gcgcagcctt gcggacggct
    21541 tgccgccgat cacaagacgc accggctgct ggcgcttgcg cttgcgcagc attttttcca
    21601 tgctggcgcg gaagtccgcg tcgtacccgc gcaggtcgtc atcgatgaac acgtcggcat
    21661 tgcgcgtgac ctgcaatagg acagcgtcgc gcacctcctg ctttttgaaa agctgcggca
    21721 caaagtggcg cacgagctcc gccgtgagca tcaccttctg ccgtccgtcc agctccacga
    21781 cgcgccacgg cgggagacgc agcagcgaaa caaggccgag ccggaaatcg tcgcccttgc
    21841 tgatcgtcgc cgccacatat atctcacggt tggcaatgaa cgggaacgga tgtcccccgt
    21901 ccacaacgcg cggcgagagg agcgggcgca gttcggagaa atactttttc gccacgacct
    21961 cctcgggctt cgtcatttta tgaaagtcca gcacgtcgat cccggcgtcg cgcatgtctt
    22021 ccagaagccg ccggtagatg cgctcctcgc gcggctgctg cgcggcgacg gcggcaagga
    22081 tggcgctcac ctgctcgccg ggcgtgcagc cggtcttttc atcccgctcg tccggcagca
    22141 gcgccgcgcg gtgcgtgagc gagccgacgc gtaccatgaa aaactcttcg agattcgagc
    22201 ggtaaatacc gaggaatttg agccgctcca aaagcggcac ggtcggatcg cccgcctgtt
    22261 ccagcacgcg ccgattgaat tgcagccagc tcagctcgcg gtcgagaaac aggcccgcgg
    22321 cctttgcctc ggcaagaagc tctgtctccg gctccgatgc ttccggcgca gcgggggcgg
    22381 tcagctccgg tcctttttcg gcttggcaca ttctttcttc actcctcggg tgnnnnnnnn
    22441 nngtgatatg ctcgttcacc gcctgctcca gcgcgtccgt cacgatctga agaacgcgga
    22501 tgcgggcgta tttcttatcc acggattcaa tgatatgcca gggggcatac gccgtgctcg
    22561 tcttttgcag catttcctcg accgccgttt catacagcgg ccacttttcg cggttgcgcc
    22621 agtcctcgtc ggtgatcttc cactgctttt ccggcgtatt ctgccgcagc tcaaagcgtt
    22681 cgagctgtgt ttccggatcg atgtggatcc agaacttgag aacgatcgtg ccgtaatcgg
    22741 cgagctcctt ttcaaactcg ttgatctcgc cgtaggcgcg cttccagtcg tcctcgccgc
    22801 agtagccctc aatgcgctcg accatgacgc ggccgtacca gctgcggtca aagatcgtca
    22861 catggccgct gcgcggcagg cgcgtccaga accgccagag ataatgcctt gcaagctcgc
    22921 ccggcgtggg gcttgcaacg gcggatgttg ccgccctttc cggcggcgtc ccagccctca
    22981 tagcagatga cgacggggat gcgcttgcgg tagatcttat tatggagcgc cgcgagcttt
    23041 tcctgcaggc gcttgagctc ggccttgtat tcctccgggg caacgctctt tgtgagatcg
    23101 gcgtcgcgga gcgaaggtgt gccgagcagc gggaaatcgt tcttccccgc cttgccgata
    23161 cagcggccgt tttccagacc gttgtcgatc gcctccgtga tggcgcgaaa gcccgcgagc
    23221 aggcgctttt tccggttccc ggcgtcaaga acgtgccatt cgccgcagac ggacgttttc
    23281 tccataaact cgccgtatgc acggcggaag gccttgtact ccttgtgctg ccagagatcg
    23341 tcgcccgtta cgcgccactc ggtatcgata ttctcgcgca gctccgcgat gcgcgagagc
    23401 tgttcctcgc gcgaaatatg cagaaagagc ttcacaacga tatatccgtt gtcgcgcagc
    23461 tgccgctcaa agatgttgca cgcatccacg cggcgctcgt attcttccgc ggtgatctcc
    23521 cggcgcaggt atttatagac gcagtcctcc atccagccgg tgtccatgaa aaggaacttg
    23581 ccgttttccg gcagcggctc gaagaacttc cggaggaacg gatagcgctc ctcgttctcc
    23641 ggcacgcgct tatagaccgc cacgcggtaa aaccgcgggt ccatctcgct gatgagctcg
    23701 ttgataagat ggccctttcc cgcgcagtcc cagccttcca cgagaacgac caccgggagc
    23761 tttgccgcca taacgctctg ctgctgcgcc gcgagccttt cgcggaggcg gcggatctcc
    23821 gccttacgct ggtctttatc gagtccgtcg tccgccggtt taaaatcgtg aagcatatat
    23881 tcgcgcctcc gaaagaagaa ttccgtttct ttttagaata tcacactgcg ttgtatctgt
    23941 caaaacctgc cgggtttttc ccaagtcaaa acggcctccc tgcggcaaag ccgtggggag
    24001 accgtctgaa ggcatggatt attccgcttt ttccgccgct tccgcgctct gctccgccgc
    24061 tttctcggcg tcaaggtttt cacgctccat caggcgcggg tcggcaagat atttcttgat
    24121 ctgcgcaaac gcctgcgcca tatagtggcc gctggcgatg cgctcgtcca tcacgacgcc
    24181 gagcggcatg caccggtcaa agacgatgcc gtccttggtg cggcgcggca cctcgcgcat
    24241 gttgcccatg gtgatggcaa tgctcgtcgt gccgaactcg tacacatggt gatagatatg
    24301 gttcgtgcgg atgcttgcga gatttgagat gagaaggctc gcgtggaacg gcgtcagatc
    24361 gatgagcgac tgtggcagaa ggccgtggtt atccagaaac cggatcaagc cgatgatcgg
    24421 cggcaggagc cagcgcagct tcgtcgtgag gatcttcatc gccgtatcaa aggagttggc
    24481 ctcgccgacc tgtcggttct ggtcgatata gccggtgatc ttcgcctgca cgtcgaacac
    24541 gtcatccgta ggcacgagat caatctttga catcgtatcg ctgccgtccg gcttgagaac
    24601 gaccatggac accttgatgt ccttgtgctg gtaaatgtta tgtcctttga tgaagcggtt
    24661 gagcgcgggg tacttttcca gcgtgtggag atacgccgtg agcaccagcg cgagatggct
    24721 gatctgcttt ccctctttcc gcttttcgtt catataggcg cgcatgggca tttccggaat
    24781 gtccagcgtg atcatgttca tggcatcata ccgctttgtg agaaaatacg ggatgagata
    24841 gtacatgggg tcttcatttt ttacaaggat tccgtccgga cgcattgttt ttcctcgctt
    24901 ccctgaatgc ataaaaattt tgatgaaatt ataccgcccg cggcgatttt tgtcaatatt
    24961 tcgtcgagcg gttcatggca aaaaatgaat tttttgttaa ttcggcggaa agttcacgac
    25021 ttatttacga tttcgtatta aagtctatga aagaagcggg cgttggaatg ccctttttcc
    25081 ccggaggtct tttttatgca gcgtggttcc cccaaccgaa attttaccgc cttcatggcc
    25141 ggccggtacg gcgcagatgc gctcggccgg tttctttccg tatgcggatg tatatgcatt
    25201 ctcctctccg tcgctgtgcg cgggaagagc gctttcttct ccgccgtatt ctccgcggcg
    25261 gcgctcgcgc tgctcatctg gtgctatgtg cgcattttct cgcggaaggt cgcagcccgg
    25321 agcgaggaaa accggaagta tctttcgtac cggaaccgct ttttcggctc gttccggctg
    25381 ctcaaaagct gcttccgcca gagaaaggag tatgctttct tccgctgccc gagctgcagg
    25441 gaggtcgtgc gcgtcccgcg cggcaaggga aggatccgca tcacctgtcc gcgctgcggg
    25501 tacgcctttg aaaagaagac ctgaagagga agtacctcca agcgtgaaaa tatgaccgaa
    25561 accgttcaag cggttaaaag agcttgccgt ctgcaaattg caggcggcaa gctcttttgc
    25621 tttgcccgaa gcctgcgatg gctgaccgga tcgcttcccg gaaagaaagc gaaatgcccg
    25681 gctcacctcg cgtccttgcc ggggccggct gtttcttcta ttctcttcac tgtcttgttt
    25741 tctgtttacc ataatatttt attgttaact taaatagcgc ttgcacggct gttatttctt
    25801 gggtataata taaaaaaata taggttcaaa ggagaaagct atggccgcag acggaaaaag
    25861 aaaaacgacc ggcgcgctcg gcgttgtttt ggcgctggcg ctgacggtcg tcgtcgcggg
    25921 aggcgctgca tacggtgcgt atgccgccgc gtccgcgcag cccggccccg ccggagagac
    25981 cgccgctgcc gcggcgcacg atacgacgct ctccgttttc gccgcgcccg ccgtgcagac
    26041 cggcgaagcc ccctcgcccg cgcccatacc gtcggaggac gccgcggaga acgggtacaa
    26101 ctataaagaa gttctctatg atgaggacgg gctgctcgta gcgcgcatcg tcggcaagcg
    26161 ctggagcggc tacatcgccg tgatcgacga tccgatgcgt gtgcaggtct ccgtctgccc
    26221 ggtgttcagt ccggacatgc gcggctactc cgtggcggag cacgcggaaa actccggcgc
    26281 ggtgctcgcc atcaacggcg gcggctttga ggaccccggc ggtgccggca agggcgccat
    26341 gcccatcggc aacgtggtgg ccaacggcga gcttctctgg ggcggctatt cctccaccgt
    26401 cggcatggac ggctccggcc agctccatgt gggagagttt tacggcggcc agtgcgtgga
    26461 gatgggactg cagtgggcct tagcttacgg cccaacgctt gtagaaaacg gcgagatccg
    26521 cccggggctg gacgcgctct atgtggagcc gcgcaccgcc gtcgggcagc gcgcggacgg
    26581 cgcggtcatc tttgttgtgc tgcagggacg ccagcctgcg gtgctcggcg tttcccggca
    26641 gcagcttgcc gagatcatgg tagaatacgg cgcggtgaat gccgggaatc tggacggcgg
    26701 cgcttcgagc gatatgtatt tcaacggcga gtatgtgaac gtgtgcaaca cgtccggcgg
    26761 gccgcggggc atccccacgg cagtcgtcgt gatgccggtg cagtaaggag gacggaatat
    26821 gaaacgctca acgcttcttc tgatctgcct tctcgtgttt gtggcgctcg tcggcggcgc
    26881 ggccgcgtgg atgtacttct cccccggcgg cgcgctcgcg ccagtcacgg cggaggacgc
    26941 cgtgcaggag cttctcgaca cgaccgatac ttccgaatgg cgcgcgaaat acgtttcgct
    27001 ctgcgcgccg gagacgagcg tttttgaaaa cgccgagacc gtcgccgaag acctattcga
    27061 cgccgccgta caggggcaaa agttttcgtt ccggcgggac agtgaaaaca cttcgcgaat
    27121 tgcgccggtt tatattctct ccgccggaga aaatgatctt ctgcgtctga acgcggctta
    27181 cgataagacc agccgcgcgt ggaaagttga acctgcgccg ctggacgcgc tgcacgccga
    27241 ggcacgcacg ctcaccatta cggtgccgga aggttcggca gtctccgtaa atggcatcgc
    27301 cctcggcgag gaatatataa cggacagtca tgttccctac accggcatat cggagcttga
    27361 aacgcagttc gacacctgtc cttaccgcgt gacgtacacc gttccgggca tttatgagac
    27421 ggcgcaggtg gacgtatcgc gcgaggcggg cgttctcctg ctctactcgg acggcacacg
    27481 ctttgactat accgtcccgg acgccgggac gcacgccttt cgcgtgaccg cgccgcagga
    27541 agcgaccgtc acggcgggcg gcgcgccgct ctccgatgcg caggcggtct cctttgcccc
    27601 gctctccacg catgtggatg ttccggacga gcttgcggga gcgctgccgg tctacacggt
    27661 atacgccgcg ggaggacttt acagcgatcc ggcctttacg gccacgctgc cggacggcac
    27721 gccgctcgag ccgatgaccg gtgccgacgg tttcccgacc tttgagctgc ccggaaacga
    27781 gacgctccaa aacgagtgcc acgagcgtgt ggagaactac atgcgtgaga tcgccggata
    27841 cggcgcgggc aacaccgcgg tgaactaccc tgccggatac gttgtgccgg gctcgccgct
    27901 gctgctctac ttccagtacg cgctcgacac actgcactgg acgcgcggct tcacggtcac
    27961 gttcaacgag gtcaaatcgt ggggctatgt cccgctcggc gaagacgcct ttctctgtca
    28021 gggcatggcg aacttcacca cagcgacacg caacgaaacg cgcgagaacc tcgtggaata
    28081 cgaaatgctc tggatccgcc gcaacggcac ctggtatatt caggatatga gctttttata
    28141 atttgaattt gcaaagcgcc gccagtcgga atgatccgat tggcggcgtt ttacggtttt
    28201 aaatgaaatg atatgttaga atagcaacct gttgtgtggt ccttcttttt atgtattttc
    28261 ctctcgactt tccaaaagaa cataattatc gagcatcaat cagaccatag tatagccgat
    28321 tttattaaca ccaataacac cacgacgaag cacagatatt tcaatggttt catactccgt
    28381 cttattgatg cttgcggtcg aagtctgtgt tctcggaata tcgatccgct caatctgcgt
    28441 ccttgggatt ggaatagtct ctacgccgag ttcgtggaga acttcatccg gcaattcata
    28501 attctgctca acaagtccaa agcaggtgaa gcccgtatcc acgcagaacg atatgagctt
    28561 cttctttcca atgttataga tgacacagaa cgaggtacca atcaagctgc cgagaagata
    28621 tcccacaaca gggagctcaa aaccaagcgc ctgaccgata atgccgccaa tatgtgcgcc
    28681 aagcagatag ccacctgaaa caacaacagt atccacaaag gctgcgccca tctgctgcgt
    28741 gctcatcttt ccagcagcaa ccagaatact gttctttacc gtctgcatca caagggcaac
    28801 gactgtacca agaacggtag gattgatgct tttgaatgct tcgccaagaa ctcctttttc
    28861 acacattatc agcagagaag aggatataga tccacggagg aaaccttctg ctcctgtgga
    28921 tattcccttc gttcccatcc tttttatctg ctgaatgtca atctctccat gcttgatgag
    28981 gaaatctatc gctttgtata tttcaggtgc aagctgaaat gctacggtga tcgccgcagt
    29041 agtgtaccca gccttcagtg cctgtttcag catatattcc gtattgatag cgctacccgc
    29101 agttaagccg tgatcttccg ccttaaactt ctgctctttg ctttcacggg cgatgtcttc
    29161 cagttctttt ctggacgcgc ttttggaact gacaccttct tcgttcttta tggtgtctgt
    29221 cagcctggat tcagtttcag cataggcatc ggaaacaccc gaacgaatct gctgattcct
    29281 caacacccca cggtgagcag ccgccttggc atcagaaagc tggtcggaag gaacgagtct
    29341 gccctggtca tgatagcttg cgtgaccgga ttctgggctg aatctcgcct gcgcttttgc
    29401 agtcttttct gccgtaacat aggatttcgc actataggcc tcgccggagt ccaattgaat
    29461 atcgacaga
//
LOCUS       DAWAAE010000006        29047 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_699_length_29047_cov_3.594785, whole genome shotgun sequence.
ACCESSION   DAWAAE010000006 DAWAAE010000000
VERSION     DAWAAE010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 29047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29047
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_29047_cov_3.594785"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14689..14698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17465..17474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5700 a         8703 c         8604 g         6020 t
ORIGIN      
        1 gggctcttta acgatctccc acggcccgaa ttcgtggggc acgatgtcgc cgatggaaaa
       61 gacgcggctc cacccgcaga cggtacattt cagttcgtag tatcctccct gcaggcaggt
      121 tggctttttc tcatcggtct gtataacgtt ttcatgatgt tccggattat tgtcgtccgg
      181 cgagcatgca agtgccgtga ccgtgagcag cgccgccgcg agcgcgagcg ccggaataaa
      241 tcgcaggagc tttttcatct ttcaccgttc ctttcaaagt atgtcgatca cgcccgcgag
      301 atacgttggc gacaccgacg catagataaa atcatcggcc agctgatcct tcaccgtctg
      361 ccgcacctcg gcggcaagct gttcgtccgc gttctcatcc accagaagga gaaccttgca
      421 gtcccacccg aggtttttta ccgcgctgca gagtctgagc cgctcgctca gcttccagat
      481 gccctgtcgg atgacctcca tcaccagtac attggcatga cagcttcggc aaagcgcaag
      541 cgtatcctcc ggttttgaag agcgatagac gatgaagtcc ggatcgtatc ccgcaagcac
      601 ctgcatgact gcctctgcca aaaggccgcc ctgcaaatcc agcacgattc ttcgcatctg
      661 tgcttctcca ccgcctttcc ctttttagag tgcttcaact atatattagc cgattgtctt
      721 ctctcgcgca cccgccgatt ggatagggtt tgcatttatt ttctgttccg ggccatatcc
      781 ccttcccgct ctccgcaaag cgcccaaaac cgcattgctc ttttcctgtc tgcatgatat
      841 actgcatttg aatgcgtgga gccgaaagga gcacggaatg agtaaagcga caaaaaagag
      901 ccttttgtat gcgcttgccg cgctggggct tatcgcgctt gccatgtggc ttcgttatgc
      961 cagccgaacg gtgctccatt ctccggttta taaccatttg cgcagcggaa tttatatctt
     1021 cctgctgtgc gcatggtgcc actctgtccg ggtccgcatt gtgcagacgc aggtgcagcg
     1081 ctatttgctg gcgatttcga tgctgatggt gctgtggctt ctcctgcgct cgatcaagtt
     1141 ttccattgcc aatacagatg cggagcgctg gctctggtat ttttactatg tcccgatcct
     1201 ctttatcccc atgctctcgg tatttgtctc ccagtcgctg ggaaagccgg aggactttca
     1261 tttgccgcgg tggacaaaac tgttgtatgt gccgacggtg ctgctcctgc tgctcgtttt
     1321 aacaaacgat ctgcatcagc gggtgttttc ctttccgtcc ggcattttgt cggacgccgc
     1381 gtaccgttat gagagaggtt atttcttcgt gctcggctgg gagctctttt gtgcggcgct
     1441 ctcgcttttt atgatcgtga caaaatgccg tatcccgcac acgaagagag tccgctggct
     1501 gccgctggtg ccgtttgcgc tttcgctggc ctatgtttat gcgtatgccg aaaagatcca
     1561 ttgggtctgg gtgctggcgg gagacatgac ggtcgcccag tgcctgatgt ttaccggcat
     1621 ttttgaaagc tgcatccaat gcggactcat ccagtccaac cgcggatacg atgagcttct
     1681 ggaagcgaca acgctgcctg tgcagatcac ggatgaaact ttttgcacaa agcacgcatc
     1741 cgctacgatg cggccgcctc ttccgcaaag tgagctgcgg cagataacgc aggataccgt
     1801 gcagcttgac gatgatacgc ttttaaagcg ccataagctg cgccgcggct gggcgttctg
     1861 gaaggaagac gtttcggagc tgaatcagct ccggcaggag ctggagctga cgtgcgacga
     1921 gcttcgcgat gtaggcgacg ttttggcggc ggagaacgca cagcacgcaa gacttttaaa
     1981 gctgacggaa gaaaaccgtc tctatgacat gatggaggcg cagactgccc ggcagatcgc
     2041 catgctgcag gagcggctga ctgagctgaa aaaaacggat gacccggcgc gggcagagcg
     2101 tctgctcgga cagatcatcg tcatcggaac ctatattaag cggagaaaca acctgatttt
     2161 tgtcggcgta cagcgcgggt caatttccgt gcaggagctg cggctctgcc tgaacgagtc
     2221 tgcggaaaat ctctgcctgt acggcgcgga gtgctcagcg ctcatcaagg gggacgggca
     2281 gctttccatt gagcaagcca cggcggccta ttcccttttt gaagcggttg tggaagcaga
     2341 gcttgaatcg ctgcgctcgc tgttggtttc catagaggtc ggcgatgcgc tgcatatgaa
     2401 tctctgtatt tccggcgagg ctcccctccg ccatctgaaa gatccattcc ccgcgcttga
     2461 atgggaagag gatgaggacg gcctgcaata tgtcatgctc cgggtggaga aatcgggagg
     2521 gaaatagaag catatggaca ggatcaaaga gagctttgac agtctgccca ttgcggtctg
     2581 cttttttgat aaaaacggcg tggtgcggct tattaaccac cgaatgcttg ccgtggcaag
     2641 ccagctgcgc aaaggcggca ttcagacgct tgcggagctg catgacgccc tgcgctcacc
     2701 gccgccaacc gtccgctgtc tggatccgca gctttatatt ttccggtttt ccgacggcac
     2761 ggagctgcgc tttgcggagg aaagcatcaa aacagcggca gggcttactt atacgcaggt
     2821 caccgccgcg gatgtcacgg agcttatgtg gcagatgccg gagatcatcc gggaggaaga
     2881 gacccttgcc atgaagctgc gtggtcatga cgatatcggt cacagcatcc ttgccgtacg
     2941 ccgcgcgctg atccggcggg ccgggttgga agagcttcgc gcaagcgcgg cgttgtggga
     3001 acagtccatc gcggtgcttt accgctccaa ccggataacg gcttcgccag aaccgatgaa
     3061 ggtggctgtc cggcgcgctg cggagatggg cgtccgggtt ttgacggagg gaaagccgcc
     3121 ggaattgcgg agtctctgcg agacggtctc gctggcaatt cgggaatgcg cggccaactg
     3181 cgcccgtcat gccggcggca cggagctata tgtacgcttt cagccgatgt gcggaagcac
     3241 cgaagttttt cttaccaaca acggcgctgc accgaaggag aaaatcacgg agggcggcgg
     3301 tctgtccatg ctgcgtcacc atgtggaaga ggctggcggc agaatggaga tacagagctt
     3361 accccgcttc tcgcttatgc tcactctgcc ggaaaaggag gaaacagcgc atgagagtgg
     3421 tgatcgttga agaccagacc atggtccgaa gcttgctgga aagctatttc cttgcggagg
     3481 acgggaatca gatcgtcgcg tccatacccg gcgcgaagca ggccgttgag gtgtgcagag
     3541 caagccccgt cgatctcgtt ttgatggatg tgcagacgga gcaccgggaa aacggattgg
     3601 ttgccgtccg tcagatcaaa gccgaacgtc cgcagataaa aattgttgtt gtgacctcgc
     3661 tgcttgacgg cgcggttttg gaggaagcaa agtcggcggg ggcggacagc ctgtggtata
     3721 aggattccgc aaaaaaccgc ctgatggagg tcgtccgccg gacgatggcc ggcgagcgtg
     3781 tctttccgga cgcaccgcct acggtggaga tcggcatggc aaaaagcacg gaatttacca
     3841 aaacggagat gaaggtgctg cggcatctgc tgcgcggcct ctcctatacg cgcatcgcag
     3901 cggagatggg ctgcgagatg tcaacggtca aattccatgt ggcgaacatg ctgcagaaaa
     3961 ccggactgga aaacaagctc cagcttgcgc tggcagtcag caacaccaag ctggtcgccg
     4021 atctgtcgga agagtaacgg cgtttgccgc acggcttgtc ccgcgttgca ttttccggca
     4081 cggccgcggc aggcgcgtga taaccgtgcc cgcgccgtcg gagccatatt attacatata
     4141 atgaatgtgc cgcaccgttt cgacaacggt gcggcacttg tttatttcac tcgtttttca
     4201 ggtctcaaac cccagcatga tcccgccgcg ctcggcataa tagctgcaca gtccccgcag
     4261 aggatcgcat ctcacatccg ccgccggaaa gcgctgccgg atctcggtgc agagctcctg
     4321 cgcgaacgcc tcgttctggc aatgaccgat ccggacgctt cctcccccgt agccgagccg
     4381 ctccatctcg cacagcatga aatgctgcgt ctttcgccct ccccggcatt ttccgagcat
     4441 ctccagcgtt ccctcgacgc tggcctgtcc gacggcgcgg atcccaagga cgcccgccat
     4501 cgttgccgtg agcctgctga tccggccgtt ctgcgcaagg ttatgcatgg attccaaggc
     4561 gaaaagcagg tgggtgcgtc tttgatatgc cttcagattt tcgcatacct cgtcaaagcc
     4621 ggcgccggag ctttgctcgg aaagcgcttt ttcaaggagg agggtgatct caggcccggc
     4681 ggaaagggtg tccaccacat ggatacgccg ggcagggttg cgctccgtgc agtttttccc
     4741 cgccacacag gcggcgttat agctgcccga cagactgctt gtaatggtaa acgcgatcac
     4801 gtcgccgctc tcgccgaaag cgctttccca gtccgcgaca ttcggacatg cgctgtagga
     4861 tcggcccctt gtgctgcgga gcgtgctgac catcgcgtca acatccagag aagcatcatc
     4921 ccggaactct tcggtttcgg tgcgtatagt aagcggaacg ctgacgaagt ccgctccgtt
     4981 cagcgtaaga agattgcagc tggaatccgc cgcaatacga aactggttca ttgtgtatcc
     5041 tccgaatcgc aaaaagcgtt ttatccggtt ttgaaaagcc gatcgggtgg attctatcag
     5101 atttttatca ttccgtcaac ggcttttaaa aacccgttaa agaattttcg caaaaccgtt
     5161 gtgctttccg tgcgaaccgt atataatggc agcatggaac aggaagtgaa gagaatgatc
     5221 gaggatatgg agagtttccg tctgccgcgc tatgcccaga tcccgaatgt agggctatat
     5281 ctggagcagg tggtgcgata tgtcaacgcc catcttgcac cgctggggga gccggaactg
     5341 acaagctcca tggtgagcaa ttacgtcaag cagagcctga tccccgcgcc aataaaaaag
     5401 acatacccat cggagcacct tgcgcggctt ttgtttatcg ccgtggtcaa acccgttgtc
     5461 ccgctggagg ggcttcggct gatgttcagc atacaggagg acagctatcc ccttccgatc
     5521 gcctacgact atttctgcga cgaatttgaa aacatgctcg gcgcggcgtt cgggatagct
     5581 cctgcgcggg aaggtcttgg tgaaacgcag tccgacgcaa aggatctgct tcgcaacacg
     5641 atctccgcca ccgtcaacaa ggtctatctg gatcggtttc tggaggagta ccaaagacgg
     5701 cgggaaaagg agcaccccgg ggcgcagcag aaatcacgga agtgattgcc gggaaaaaag
     5761 gatagcagca agcctgcctt atctccgatc gaaaggacgg ttcagcttcg gctgtaccgt
     5821 ccttttctcc ttcctgcgcg ctgtccctgc agcctttatc ggcggcggga gcgttccgtt
     5881 acggccgctg atcgagcgca tagcttgcgg aaaacgcctc tttcgttgaa acgcgatccg
     5941 gtcgtgccgg gctttgcctc gcacgaaacg ctgctctgct cccccgagcg gaagttctgc
     6001 tccaaccgcg tgaagacaga cgccgatctc aacacgaaca tccgccgttt ccactgcctc
     6061 ggcgactcgt ccggctggac gcgctggcgg atgacggcct ccgtcatggg cgtgctcatg
     6121 ggccgcgaga tcatccggca ggaaaacatt tgattatata ataatccaaa cgcatacggt
     6181 cgcctcccgc gggaatactc ccacgggagg cgatttttat gaatatgacg aacgaagaat
     6241 acaatgaata tatcaaaaag aagcagccgc gctcgccgct cggcatggac atgctgcgcg
     6301 cttttctcgt cggcgggctc atctgcgccg ggggcgaggg gctgcgcaca ctctatatct
     6361 ccctcgggct ggacacggaa agcgccggga cggcggtttc gatctcgctc gtgtttctcg
     6421 gcgcgctgct cacaggtctg gggctgtacg acgatatcgc gcgctttgcc ggagccggca
     6481 cgctcgtgcc gatcacgggc tttgccaact ccatcgtcgc gcccgcgctg gaattcaagc
     6541 gtgagggtct catccccggt acctgcgcca agatgttcac gatcgccggg cccgtgatcg
     6601 tttacggcat cggcgcaagc gtcgtttacg ggctgctgct ctgccttttc gcctgacggc
     6661 gggcgctctc ggaagaaatc gcaaaaaagc ggagtttttt gcattttagg gttgacattc
     6721 tcccgtgcat ttgctattat aactctcgcc ggttggaaaa ccgtgttcct gtgggggtat
     6781 agctcagctg ggagagcgct tgaatggcat tcaagaggtc agcggttcga tcccgcttat
     6841 ctccaccaaa aagcaccgaa atcgcttgat ttcggtgctt tttctttatt ttttcccttt
     6901 gttttcagaa aatcctatct gttgcgcacg tattatctgc agacaaatca cgccgcgcgc
     6961 cggaacgatc ccggcgcgcg gcgcttttgc tttttgctct ttatttcacg ccgaacatca
     7021 gctcggcata ggtcgggaac ggccagctct tcgcggggat gagcttttcg gccgcgtcgc
     7081 agggctcgcg cagcgccgcc atcttcggca ggacgctgtc gcggatgaac tccgcagcgc
     7141 tctgcacgtc gctgatggcg tggtaagcct cgaccgccgc ttccagctct tccgccgccg
     7201 cattgatctt ttcagtgagc gcggagagct tttcgatctg ccgcgcctca taaccgccga
     7261 cggtcgtgcc gaccgcggcg cggcgggcgt tcacgccgag agcgagcgta tcagcatacg
     7321 ccgtcacggc gggcaatatc tcgcggcgcg ccatatcgag catcgttttt gcctcgatgt
     7381 ggacggtctt gcggtagtta tcgagattga tctcgcagcg ggacttcagc tcggtctcgg
     7441 aatagacctt gtggcgcgtg agcatttcca cgttcttcgg cgcggtgaga tacggcagcg
     7501 cgtccggcgt cgtgcgcaga ttgagaaggc cgcggacctt tgtcgcctcc tcgatccagg
     7561 cgtcgtcgta gccgttgccg ttaaagacga tgcgcttgtg cgcgcggatc gtgcgctgga
     7621 tgagcgtgtg gagcgtgcct tcaaagtcgt ccgccttttc cagctcgtcg gcgtactggc
     7681 gcagcgactc cgccacggcg gtgttgagca tgacgttgca gcacgcgatg gagtcggacg
     7741 agccgaccat gcggaactca aagcggttgc cggtgaacgc aaacggcgag gtgcggttgc
     7801 ggtcggtcgt atcgcgcacg aagcgcggca gcacatgcgc gccaagcttc ataatgatct
     7861 tttcggtgcc gctgtagggc gcgtccttct caatcgcgtc gagcactgcg gcgagctcgt
     7921 cgccgaggaa gatggagata acggccgggg gcgcttcgtt cgcaccgaga cggtggtcgt
     7981 tgctcgccgt tgcaacggag agacggagca gatcctgata atcgtccaca gcctggataa
     8041 cggcgcagag gaacagcaga aaccgcgcgt tctggtgcgg cgtgtcgccg ggcttgagga
     8101 ggttcacgcc cgtatcggtg cagagcgacc agttgttgtg cttgcccgag ccgttcacgc
     8161 cgtcaaacgg cttttcgtgc agcaggcaca caaggccgtg gcgcgcggcg acctttttca
     8221 tgagttccat ggtgagctgg ttctggtcgg tggcaatatt cacggtcgta aagatgggcg
     8281 cgagctcgtg ctgcgcgggg gcaacctcgt tgtgctcggt cttggcaagc acgccgtgct
     8341 tccacagctc ttcattgaga tcggccatga actccgcgac gcgcggcttg atcgcgccga
     8401 agtaatggtc gtccatctcc tgtcccttcg gcggcattgc gccgaagagc gtgcggccgc
     8461 agaacataag atcctcgcgc ttatcgaaca tggcgcggtc aacgaggaaa tactcctgct
     8521 ccgggccgac gagcgggcgg actaccttca catcgtccat gccgaagagc ttcagtacgc
     8581 gcagcgtctg cttgttgagc gcctgcatgg agcgcagcag cggcgttttc ttatcgagcg
     8641 cttcgccgcc gtaggagcag aacgccgtcg gaatgcagag cgttttgtcc ttaataaaag
     8701 cgtggctcgt cgggtcccag gcggtgtagc cgcgcgcttc aaacgtggcg cgcagaccgc
     8761 cggacgggaa gctggaggcg tccggctcgc cttggatgag ctccttgccg gaaaactcca
     8821 taatgacgcc gccatccggc gagggggaaa tgaagctgtc gtgcttctcc gcggtgctgc
     8881 cggtgagcgg ctggaaccag tgggtataat gcgtcgcgcc cttttctacc gcccattcct
     8941 tcatggcgtc ggccacggcg ttggcgaccg ccgtatccag cgtgccgcct tcatacatcg
     9001 tctttttcag cgagacatac acgtcatacg gcagtttcgc tttcatcacg cggtcgtcaa
     9061 acacaagact cccaaaatac tccggtacgg tgctcatgca tcccactcct ttttttcgga
     9121 aaaaaataat gaaaaaggcg tctcggagat cgctctccaa gacgcctttg ccttcgatgg
     9181 gcgaaattat atgcgccctg cacggatttg tcaattccct attttcgatt ttttgcggtt
     9241 tttttcgttg acgcgctttt cacgcggtga tataattttc cgcgttaatg agcaaaggag
     9301 ttcatttccc gtgggaagtt tatcccctcg ggaaatgagc tccttttgta tttttcggaa
     9361 agaaggcgat tattatgaag cgcgaaggcg agatccgcat cccctccggg tgtgccattt
     9421 ccgccgtcat ctcccgggag ggacggcgca tgaccggcga ggcggtcatg aaaagcatga
     9481 tcccgatgca cgaccgctcc aacggtctcg gcggcggctt tgccgcctac ggcatctacc
     9541 ccgactaccg cgatttttat gcgtttcaca ttttctttga cgacaacgcg acgcgccgcg
     9601 agtgcgaagc gctgctcaaa gagggctttg agcttgtgca ggccgagcag atccccatcc
     9661 acatcatccc ggagatcacc gacataccgc tcatctggcg ttactttgtc tccccgctgc
     9721 cgtcggtgct ccaccgtctc cagctcgacg agaaggagtt cgttgcccgg accgttatgg
     9781 acatcaacac gaagttcaag ggcgcgtacg ttttctccag tggcaagaac atgggcgtat
     9841 tcaaggccgt cggctatccc gaggacgtgg gacggtttta ccggctcgac gaatatgcgg
     9901 gctacagctg gacggcgcac ggccgctatc cgacgaacac ccccggctgg tggggcggcg
     9961 cgcatccgtt tgcactgctc gactactcgg tcgtacacaa cggcgagatc tcgtcttacg
    10021 acgccaaccg ccgcttcatc gaaatgttcg gctacaaatg cacgctgcag accgataccg
    10081 aggtcatcac atacattgcc gattatttgc tccggcggca gggcatgtcg ctggaggaag
    10141 cggccaacgt catcgccgcg ccgttctgga gcacgatccg cggcaaagcc gcgtctgagg
    10201 ccgaacggct cacctatctg cgcacggtgt tttccagtct tcttattacg gggccgttct
    10261 ccatcgtgct cggcttcaac ggcgggctga tggcgctcaa cgaccggctg aagctccgct
    10321 cgatggtcac ggcggagaaa gacgatctcg tattcattgc aagcgaggag gccgccatcc
    10381 gcgcgatgga accggacgcg cgcaacgtct gggcgccgat gggcggcgag ccggtcatcg
    10441 tgcgcgtaaa ggaggggaaa tattaatggc tatcgaatac atctacccgc cgtttgaggt
    10501 catccggcgc gacgagcggt gcacgcgctg ccgcatctgc gaaaagcagt gcgccaacgg
    10561 cgtccacacc tacgacgccg agcgaaagat catgctcgca gacgaatcca aatgcgtaaa
    10621 ctgtcagcgc tgcgttgcgc tctgcccgac gcgcgcgatc aagatcgtaa aaaccgactg
    10681 ctgtctgcgc gaaaacgaga actggaagag cgacacgatc cgcgaagtct ataagcaggc
    10741 cgccacaggc ggcatccttc tctcctccac tggcaacccg acgccgctgc cgggctactg
    10801 ggaccggctt ctcatcaacg cctcgcaggt cacgaaccct tcgatcgacc cgctgcgcga
    10861 gccgatggaa acgcgcgtct ttctcggaaa aaagccggac acgatccgcc gccgcgccga
    10921 cggctcgctc gacacgacgc tcccgccgca gctggagctt tccgtgccga tcctcttttc
    10981 cgcgatgagc tacggctcga tcagctataa cgcccacgct gcgctcgccc gcgccgccga
    11041 ggagctcggc atttactaca acaccggcga gggcggactc caccgcgatt tctacaagta
    11101 cggcgcaaac acgatcgtgc aggtagcctc cggccgcttc ggtatcgagg agcgctgtct
    11161 gaacgccggt gccgccatcg agatcaaaat gggtcagggc gcaaagcccg gcatcggcgg
    11221 acatctgccc ggcgcgaaga tcatcgggga cgtgtccaag acccgcatga tccccgaggg
    11281 ctcagacgcc atctcccccg cgccgcatca cgacatttat tccatcgagg acctgcgcca
    11341 gctcgtatac tcgctcaaag aagcaacgca gtataaaaag cccgtcatcg taaaggttgc
    11401 ggcagtgcac aacattgccg ccattgcgag cggcattgcc cgcagcggcg cggacatcat
    11461 cgccatcgac ggctaccgcg gcggcacggg cgccgcgccc acgcgcatcc gcgacaatgt
    11521 cggcattccc attgagctcg cgctcgccgc cgtggatcag cgcctgcgcg acgaggggat
    11581 ccgcagcagc gtttctctca tcgcgggcgg cagcatccgc tcgtccgccg acgttgtcaa
    11641 ggccatcgct ctcggcgcgg acgcctgcta catcggcacc gccgcgctgc tcgcgctcgg
    11701 ctgccatctt tgccgctcgt gccacacggg acggtgcaac tggggcatcg ccacgcagaa
    11761 tccggagctt gtcaagcggc tggatcccga cgtcggcgcg gagcggctcg tgaatctcgt
    11821 cacggcatgg aagcgcgaga tcaaggaaat gatgggcggc atgggcatca actccatcga
    11881 ggcgctgcgc ggcaaccgcg tgatgctccg cggcatcggc cttacggaga aggaactcga
    11941 aatcctcggc gtggcccacg ccggagaatg attgtggaaa atcggtgaaa ggtgtgatac
    12001 tatggattat atagaagcag gctctctctc cttttccgag ctgaacgaga cgatccgcag
    12061 tgccggggcg aaatgccgcg tgagcgcgtg tctcggccag cggttcatcg ccgcgggcat
    12121 gtcccgcaag agcattgaga tcgacggcat ccccggcaac gcgctcggcg cgtatctcaa
    12181 cggcgcggac atcaccgtgc acggcaacgc gcaggacgcc gtcggcgata cgatgagcgc
    12241 cggacggatc gtcatccacg gcaacatcgg cgacgccgcc ggttacgcca tgcgcggcgg
    12301 agagatcttc gtggagggca acgcgggcta tcgcgccggc atccacatga aggaatacaa
    12361 ggaacacaag cccgtcatcg tgatcggcgg gcgtgccggg agctttctcg gtgaatacca
    12421 ggccggcggg ctgatcgttg tgctcgcgct gcatacggac ggaaaacccg tcaccggcta
    12481 tttcccctgc accggtatgc acggcgggaa aatggttttc cgcggcgacg tgtcgcgcgt
    12541 gccgttcccg agtcaggtga cggcgcatgc cgccacggag gcggacaagg cagagttcgc
    12601 gccctatgtg cgcgagtact gccgcctgtt cgggcgcgac gcggatgcca tgctccgcga
    12661 tccctacacc gtcgtcacgc cggacagcaa aaccccctat aagcagatgt acgtttccga
    12721 ttgagaaagg caggagcagc aatgaagtat tccgagtccg agatccggca gtatgtcgcg
    12781 gaggaggacg tcaagttcct tcgcatgacg ttttttgacc tgaccggcaa ccagaagaac
    12841 atgtccattc tgccggatgc gctcgaccgc gcgttttccg ccggcatcgc gtttgacgcc
    12901 tccgctatcc ccggcttcgg cgacgagctg cgctcggatc tctttttgca gccggacacc
    12961 tcgacgctct gtctgctccc gtggcggccg gatcacgggc gtgtggcgcg cctgttctgc
    13021 cgcattctgc gctcggacgg cacgcccttt gagggtgaca gccgcgcgct gctgcaaaag
    13081 gcggtggacg acgccgcggc gatgggcgtt tcgttcgcgt tcggttcgga gatggagttc
    13141 tatctcttcc gccgggacga aaacggcgaa ccgaccacga ccccctacga ccgcgccggg
    13201 tacatggaca ttgcgcccga agataagggt gagaacgtcc gccgcgagat ctgcctgacg
    13261 ctcgaacaga tgggcatccg cccggaatgc tcccaccatg aacagggccc cggtcagaat
    13321 gagatcgatt tccggtatgc cgagcccgtc aaggccgcgg acgacgcggt cacgttccgc
    13381 acagtcgtaa ataccgtcgc cctgcgcaac ggtcttgccg ccgacttctc cccgaagccg
    13441 ctgcgcagcg aaccgggcaa cggcatgcac atcaatctct ccgcccgcag ctccaccggc
    13501 gaggacatta tgccgcaggt gatcgccggt attctctcgc atattgccga catgaccgcg
    13561 ttcctcaata ctacgccggc ctcctatcag cgcttcggca gctacaaggc gccgcgctat
    13621 atttcctggt caagcgaaaa ccgctcgcag ctcatccgca ttcccgccgc cgaaggcgag
    13681 taccgccgcg cggagcttcg atccccggac ccgatctgca acccgtatat tgcctatacc
    13741 ctcctcatct acgccggtct cgacggtata cgccgccgcg cagagctgcc cgcaagcgcg
    13801 gacatcaact tttacaccgc gtcggaggag gttcgttcgc agtataagac gctgcccgcg
    13861 acgctcgcgg acgcaaaagc cgcggcaaaa acgagcgaat ttatcgcctc ctccctgccg
    13921 cgaaccgttg tggagtatta tacgaagtaa gcgtcgccaa aactttcggg aagaggaatg
    13981 cgcatggagt atctgccgcg aacctacagc gtgctgtccg tagccgctgc ggaaaaattt
    14041 catacgtccc tgcgtgagct gctccccggc gggcgctacg cccccgtcca atttgcgtac
    14101 gacgctgcgg acgcacggcg ccggcttctc gaaagccggt atgatattat catcatcagc
    14161 gcgccgctgc gcgatgaatt cggcactcat cttgcacagc acttcgccga gcaatcgggc
    14221 gcgggaatat tgctcctggt aaagccggag tattactccg atgtgtccgc gcaggtcacg
    14281 ccctgcggcg tgctgacgct tcagaagccg acgtcgccgc agatgatgct gcaatgcatg
    14341 gagctcctgt gcggcacgcg ggagcgcctg cggcgcatgg agcagaaaag tctttccatg
    14401 gaagataaaa tggcggagat ccgccttata aaccgcgcca aatgggcgct gatagaaaag
    14461 cagggcatgt ctgagcagga cgctcaccgt tacatcgaaa agcaggccat ggaccgctgt
    14521 gtgccgaagc gcgtagtggc cgaagaaatc ctgcaaaaaa aggaatagta gaaaaaaacg
    14581 gatatgcccc ggcagggttt cctgtcgggg catatccgtt tcagcctatc gaaaaagccc
    14641 cgctttggct ggactttttc gacaggctga aggcaggtcc ctttttttnn nnnnnnnnga
    14701 acgtggacag ctgctggatc agcacaagga atacctgtac gaccagcagc gcgatcacgg
    14761 gaccgacaat cgccttacag aggttcttga ccggtatgcc ggtaatggaa gccgctgtat
    14821 aaagattcgt acccaccggc ggtgtaacga agccgatagc gaggttggcg accatgatga
    14881 cgccaaagtg gatgggatcg atgccgatgc ccgccgcaac cggcatgagg atcggagcaa
    14941 ggatcgtaat ggcggacaac gtctccatca gcatgccgac gatcaacagc aggatgttca
    15001 gcagcagcat gatcatccac ttgttgctgg taacggacag gatggtagcc gccagcgcct
    15061 ccggcacgtt ctggatcgcc agagcacggc ccagcacggt ggccgcagcc acgatgatga
    15121 ggattggcgc attgagacca gcggcgcgga gcagaatacc gggcagctgc ttgaacgtga
    15181 ccgtccgata gatgaacaga gatacgatca gtgcgtacac gcacgccacg gcggcggctt
    15241 cggtcgcggt gaaaaggccg ctgtaaatgc cgccgaggat gacgaccggc gtcagaagag
    15301 cccaaaagcc gtccttgaga ctctcaaaga tgcccatggt cttctccgtg tggtcgatct
    15361 tcaccgggtg gcgcttacag tagatatagc agtaaaccat cagggcaatg ccgcagagaa
    15421 cgccggggat gatgccggcc gtcagcatcg agccgatgct gaccgaaccg accgtaatgg
    15481 cgaacatgac catcggcagg ctgggcggga tcagaacgcc caggccgcca gaggaggccg
    15541 ttacgccggt aacgaagttt ttatcgtagc cctgctcctc catggcgggg atcatgatgc
    15601 tgccgaccgc ggcgaaggtc gcctgaccgg agccggagat cgcaccgaag agcatgcagg
    15661 tgagcaccgt cgccatcggg acgccgccgg taaaacggcc cagacaggcg ttggcgaagt
    15721 tgaacagctt tttggaaatg ccgccctggc ccatgatctc accggccaga atgaacagtg
    15781 gcaccgcgag gatcgtaaag gagtccagcg cctgcaccat gttacggcca atataggcga
    15841 gggagaccgc gccgcccgca aggccgttgg cgatcacgcc gaggccgatg gagcccagca
    15901 ccggaacgcc gatcgccagc aggcagaaaa ataccaaaaa cagaattccg acagcagccg
    15961 tcatttccct tcaccttcct ttttcttttc cctgtactgg cgccacattt tctgcagcac
    16021 ccggaacgtc gtcaggccca tgcccaccgg aagcgccaga tagacataca gaatagggat
    16081 gtgcagcacc gtgctgtact gcggcatggc cgcgaacttc gagcagattt gaatgcctac
    16141 taccgtcata gtcacggtga atacgaggaa aatgacatag gagaccgtgt ggatcacgcc
    16201 gcggccctta tgaatggacg tttccagaat atcgacccgg atgtgcgtcc cctttttcgc
    16261 cgcaaaggcg cttcccatga aggtcatcca gatcagggtg tagcgtgccg tctcctccgt
    16321 ccagggcatg gagatcttca gcacaaggcg cgcaaaaacc tgcaaagccg tggcaaacac
    16381 aaaaaatgcc acgaagcagg aaagtatcac ctcttcaaag taatgatcca gataatacaa
    16441 tacttttttc atccaatttt cctttctcgg cgcgcgagaa gccgcagcaa gacagggtct
    16501 tgctagttag gtccgctgcg gctcttttcg ctcagcgcat caggccgcag cctcgatggc
    16561 gtccttcagc tcgttaacga gatcggcacc cacgttggcg gcgatgacgt cctccgcgct
    16621 gcccatgaga gcctggaact gggccagctc gtcatcggtc agggctgtga cggtcagacc
    16681 gtcgttctcc aaagtggtga tgtagttttc cttctgggcg gcggcttcct gacgctggaa
    16741 ttcaacaccg gcgcggatgg catcgagaac gacctgctga tcctcagcgg agaggctgtt
    16801 gaaccaagcg gggttgcagg tggccatgcc gacctggaag atgtggttgg tcagggtgat
    16861 gtacttctgg acctcataga acttttggga gataacgagc tcgagggggt tatcctgcgc
    16921 gtcaacggtg ccctgagaca gagcggtgta aagctcaccg aaggcgatcg cggagggaat
    16981 ggcgccgagg ctctcccaga tggcgatgtg caggttgctc tgcatcacgc gcaccttcat
    17041 gcccttgaga tcggcggggg tgtggacttc cttgttggag ctcagctcgc ggtaggtgca
    17101 gctctcgaag tagccgaggc ccttgagacc gcaagcgctc tccatggagt cgagcatctt
    17161 ggtgccgaaa tcgccgtcga gaacggcgta ggcggtctcc tcattgggga acgcgaacgg
    17221 cagggagaag acctcgagat tctggttgaa ggaggtcagg ttggaggtgg agcaggcgga
    17281 catctgaatg tcgccgctct gcacggcctg cgcaaggtcc acgtcggagc cgagcgtgcc
    17341 gttggggtaa atggtgcact caaagttgcc gttggcttcc atgtaatcgg cggccacgac
    17401 ccaagccttg tgcatcaggg aatcctcggt accggcgtgg gcgatcttca cagcgatctt
    17461 ctttnnnnnn nnnntcggcg gaggtgtcgg tggtggtgtt gtcggtgctg tgggtgctgg
    17521 tgctctcgct gccgcaggcg gtcagcaggc tcacggccat cagcaggacg agtgcgaggg
    17581 aaatgactct tctcattctc ttgttcatgt tcgtgctcct ttttgttttt tatttagtgg
    17641 tacgctttca gacttacgcc tgaaattcct ttacttcttt caccctgggg ttcacgatgt
    17701 tggccgtcca cttgccggac agcacgtcga gcgcctgccg cgtcagcttc aggtgtaggt
    17761 cgatcatggc ttcctcagaa tacatcgcca cgtgcggcgt gtagatgagg ttcgggatct
    17821 ggtgggcggg atgctcgggc ggcagaggct cgccctcgat aacgtcgaga ccggcgccgc
    17881 cgatgatgcc ctcgcgcagg gccgtcagca ggtcctcggt gttgatgatc gcgccgcggg
    17941 atacgtttac aatcacggca tcctttttca tctgtttgag ctcgttcata ccgatcatgt
    18001 ggtaggtacc ggggttgagc accgtgttga tggaaatgat gtccgaattg cgcagcactt
    18061 cctcgagcgt actgcaaagg cggatgccct tctgatcgat ctccttgagg aagggatcgt
    18121 aaacgagcat ctgcatgccc atgccgtgga ccattttggc ctgctcgcgg gcgatgcggc
    18181 cgaagccgat ctggccgaac acctgatcgc agagacggtg cacgatgtgg ccctcgccct
    18241 gaccgtagcc gccggccttg ccgcgggcca tgaagtcgag caggtgcttt ttcaacgtca
    18301 gcgccaacag gaaggactgt gtcgctacct cgtaggcgca gtagtcgggg acattgcaca
    18361 cggggatgcc gcgctcggtg gccgcgtcga gatcgacgga gtcgtagccg atgccgcacc
    18421 ggccgatgaa catgagcttg ggcagcgcct cgatcacgcg gcgggacatg ggcacggcca
    18481 cgaccatgac cgcatcggcg tcctgtgcgc ccgcgatgat ctcctcctcc gtggaccagt
    18541 cgtggaactc gatctccatg ccggcctttt caaaggcttc ctgctcaatc ttcttggtgt
    18601 cgggtcccag ccactgctga tcgacaaaat gaatcttcat aatcggatcc tcctcacatg
    18661 gtcagggctg cggaggagcc tggagaccgg actcttccgc gcctgatgcc atgaaaaatt
    18721 attactcctg gcagagcttc tgcagtctca cgtagcgggt atcggtcagc tgctgcagac
    18781 cctcgatgtc ttcctcggtc agatggcgga actttttgaa gcgggtcacg aagtccttga
    18841 cgggcttcgg atccttcacg gtgacggaca tggagaactt gccgttttcc acctcgtaga
    18901 ggggggcgaa gttggtctcg acggccagac gcgccacctc gatgctctcg tcggacttgc
    18961 agccccagcc ggtaacgcag gggttgtaaa cgtgcagata cgcaagaccg ttatcgacct
    19021 gcatggcctt ttcgatcttc ttgaccatgt cggggatgta ggccgggctc atcgtggcca
    19081 tataggggat gtcgtgcatg gccatgatca tcgggaagtc cttcttgttc tgcttcttgc
    19141 cgccgtggcc gttgatgacg ggcgtggtgg aagtccacgc gcccttcgag gtcgagccgc
    19201 tgcgctggta gccggtgttc atgtagcctt cgttgtcgta gcagatgtag atcagcttgt
    19261 cgccgcgctc agccgcgccg gagagacact gcatgcccgc gtcgaccgaa gcgccgtcgc
    19321 cggcgaaggc gaccacgttg acgtggcggc caatgtgctc atagtgcagc ttgatgccgg
    19381 ccagcatgga ggcaacgtta tccagcagtg ggaaaaacac cggggtcttg gcgccggact
    19441 gcttgtccca gccgacgacg ccgacgccac ccatgcatcc gggaggaatg gcgaagatcg
    19501 tgttggggcc aaggaccttc atgcaggtac gcagtgtcag ctcaatgttg cagccgacgc
    19561 acgcggagat acccggggag atctggtctt cataggttgc cagtttatca atgacattca
    19621 tggttgttcc tccgttcctc aaatgcccat gtccttccgg ttgagccaga ttgtctgatg
    19681 acgctcggtc ttgccggtct gtccaatgat catttcgata gcgcggtcga tgtgctcgcc
    19741 ggtgacatcc tcgccgccga gacccgcgat gaacgggata ttcggcacga agctgttggc
    19801 acggtacatc gcggcgcaga cctcttcata cgtaatgccg gtgttccagc cgaaggacac
    19861 gttgcggtcg atgacgccgt aggccttccg tccggccaga acggccgcga cgcgctccgc
    19921 cgggaagggg cggatcatgc gcagcttcag aaggccgatc ttgacgccgc ggctgcgagc
    19981 cgcatccacg cggtccttgg caacaccgac catggagccc atggtaacga ggatataatc
    20041 ggcgtcctcg cagcggtatt cctccaccgc gccgccgtag gaacggccga acttctcgcc
    20101 gtattccttg tcgagctgct cgaagagctt cagggcgttc tgctgggcca tcatgtgctt
    20161 cagacggtac tccgtcagaa gagcgccggt ggtcagcggg tcgacggcca tgggcttgag
    20221 cgggtcgagc aggatgtgct ccgggtgata aagcggcagg aaagcgtcga cctgctcctg
    20281 gtcggggatc tccacgcgct cggacatgtg ggagagatag aagccgtcga agcagacgtt
    20341 gatcggcaga aggacgttct tgtcctcggc cagacggtag gccatgatca tggtgtcgag
    20401 aatttcctgg ttatcttcga catagacctg cagccagccg gcgtcacgca cggtcaggga
    20461 gtcctgcggg ccgccccata gggtctgcgg ggagatcatt tcacggttgg ccacgcccat
    20521 gacgacgggc agccgcagcg tggaagcgcg gaaatagggc tcatacatca gagataggcc
    20581 ctgcgacgcg ctggcggtga acacgcggga gccggccaaa gacgcgccgg tcacgacgct
    20641 catggcggag agctcagact ccacctcgct gacggtgctg tgcagctcgc cgccggtcac
    20701 gaagtccgcc agatactcca cgatgggggt ctgtggcgtg atgggatagg ccgcgatgat
    20761 gtcggggcgg cagagtttcg cgccgatggc cgccgccatg tttccgttga taactttaac
    20821 cgtagccatt attcttcctc cttcaccggt tccatcgcaa tggccttctt cttacactcg
    20881 tgagcgcaga tgccgcagcc cttgcagtaa tcgtagcaga tctcaaagga accgtccgcc
    20941 agcttgcgga tcgagttgac cgggcagtac atgaagcaca tgccgcactt gatgcacttg
    21001 ctcttatcca tcacgggctt tctggtgcgc cacttgcccg tgtcgacaat cttgagccct
    21061 tccttgccaa taggaacaat gtaatcactt ataaactcca taatgccacc tcacaaatca
    21121 gctgaagtca aacagtttca cttcgtcata accgcggcgg aacgtctcca cgttcttttc
    21181 gccgaagaac tggagaacta ccttctccag agcctccttg tcgacccagc ccgtggcctt
    21241 cacgaaagcg ccgagcataa tggtgttcgt gatggggcgg cccagcacct caagagcgat
    21301 gctggtggcg tctagccacg ccacgcgctt gacgttgggg ttggtgataa ggggacgggc
    21361 atcggccgcc ttggtgttga gaacaacaac ggagccttcc ttcatgcctt cgaacaccgg
    21421 cgcggaaatc agcagactgg ggtcgaggat cagcgtgcag tcaggattgt agacctgatt
    21481 cttcgcgcgg atcttctcat cgctcagacg cacaaaggac gtaacgggcg cgccctgacg
    21541 ttcgaagccg aacatcggcg tggagtttcc gtacttcccg cttgccacgg tagcatgtac
    21601 cagcatctcg ccggccttca cggtgcctag tcccccgata ccgtggagcc tgatctcttt
    21661 catttagatt cctcccaaaa ttgatatatc gatcaatcct ctaagatgag catcgcgtct
    21721 accgcggtag ctttccggtc gccgtacagg ataaacggat tcacatcgat ctcgcggatc
    21781 tccgggtgcg cctcgcagag cttcgacaga ctcagcagcg cctccacgat ctcctcgcgg
    21841 tcatatttca cgccgcgcag accctcgcag gccttcttca gcggcgtttc gtcgatcatc
    21901 tcctccgcgt ccgtgcggga gacgggcacc acgcgcatgg agaccgcctt gagcagctcc
    21961 acgtaaatgc cgccgagacc gaccatcagc acctgaccga actgggggtc cgtgctcacg
    22021 ccgagcagta cctcaaagcc cttgggaagc atctgctgca ccacgacgcc ctgaacgcgg
    22081 gcgttggggg cgttcctgca cgcgtttgcc agaatggtct cgtatgcctc cttcatctcc
    22141 tctacggagt ggatgttcag gcagacgcag cctagatcgg tcttatgcag aatgtcgtcc
    22201 gacaggatct tcatgacgac cggaaaacca aggtcctccg ccgtctggcg gagcgtggcg
    22261 cccgagtcct tcgtcagcac gacggatttc accacattga cgccggcgga gccgagcagc
    22321 ttcgcgctct cgagctcgtt gagagatctc ttagccatgg ttctcctcct tttcgcagcg
    22381 gctgcggaac tgcgcgtatt tgcagagatt cgccgccgcc tttaccgcct tctccggcgt
    22441 gttgaacgtg cagaggccgt tgctctccat gattttgcgc gccttggtcg tataggctcc
    22501 ggccattacg cagtaataga ccggtttctt tttctccgcc gggatctcgc ggtcgagcgc
    22561 cacgaagccc tcggccagct cgacctgcgg caggaaggtg ggaactaccg tgacaagcac
    22621 ggcggcgtcc acattctcgt cctcgagaac gatgcgcaga ccgtccacat ggttcttgac
    22681 agaggccgac gccgtgatgt ccgcgtagcc ctgcggatgg cagatgttcg ccatcgggag
    22741 ctgggcctcg gtcagcttct tcttcgtctc gtcgctgacg tccggcatcg tgaggccgtc
    22801 ggccgcgcag atggcgtccg tgcagtaaat gccggggcct ccggcctgcg tcagcaggaa
    22861 ggtgttgccg cccgcgggca ggggcatctg gtcaaaggcc tgcatggtgt cgatcagctc
    22921 gtcgatcgta tccacgcgga taacgccgca ctgcttgaac accgcgtcat agacctgatc
    22981 ggaaccagcc atggagccgg tatgggagtt ggccgcgttc gcgccgagag cgctgcgtcc
    23041 gaccttcaaa accacgatgg gctttttccg gctgacgcgc ttgaacgttt cggccagctt
    23101 cttgccgtcc ttgtagcctt ccatgtaaat gccgatgacc ttggtagtct cgtcggtctc
    23161 gaggtaactg ataaggtcga taaagtcgat atcggccatg ttgccgatgg agatgaactt
    23221 gttgtagagc accggcacaa cgcgccccgc cccttccatc agcaccgcgc tggccagcgc
    23281 gccgctctgg gagatgaagg tgatgccagt cggtgcgccg aaggtggaaa acggcacgcc
    23341 ggtatccacg aacgtggtgt cgaccagact gcggttatcg ataacgccga tgcagttggg
    23401 gccgatcacg cggatgtgat atttctccgc cgtttccatc agccgcttct gccgggcgat
    23461 gccctcttcg gtcttggtct cggcgtagtc tgccgcggcg cagacgatga atttcacgtc
    23521 acccttggtc tccatgcgcc gattcagatc gtccatcacg tcgtagatca ggcgggatgg
    23581 catgatgagc accacgagat ccacgttctc ttcaatgtcg gccagagagc cgtagcaggg
    23641 atacccgaga atttccttct ccttcgggtt gatcggaaac accttgcccg ggtattcctt
    23701 ttcccgcatg ttgcggatcg ccacatagcc ggccttcccc gggtttgccg aggcgcccac
    23761 caccgcgatc ccgttcggat tgaacagttt gtccatatac taaaatcctt tctgcttggg
    23821 atgctgggta ctcaatgctg gagcatttct ccgcgaagcg cgtcgtctac gatgcgacca
    23881 tcgagcgccg cgattcgtcc gccctgaata gtacacaccg gccagccgcg cagctcctct
    23941 ccctgaaaga gcgaaaagtc cgagcggctc tggagcgttt tggggtccgc gcggcgcacc
    24001 gtttccatat ccacgatcac gatgtccgcg tccgcgcccg gcagcagcgt gccctttttg
    24061 gggtagacgc cgaagatctt ggcgggattc atcgttatca ggggaacgag cttttccagt
    24121 gggatctcct gccggaaaaa accctcgttc agaacggaga caagatgcgt ccccagcgct
    24181 ggatagcccg cgtcagcccc ggccataccc tgcggcatct ttttctcggc agaggtcatg
    24241 gtcacattgt ccgttccgat cgtgtcgatc aggcccttcc ggatgccgtc ccacagcgcg
    24301 agacggcttt ccggctcgcg gaagggcggc agcattttcc cgtacgcgcc gaccttgctg
    24361 gaggtgtccc gggtcagata gggcgaggtc gtctccacat agaagcgctc gtgcttcgct
    24421 ttggacagga cctccatgct ctccttgctg gactggtgca ccacatatgt gcgcacgccg
    24481 gtctttcctc cgagatatgc gcagcgaagc accgcttcgc cctcggcgat gttgggatgg
    24541 gtcttgcgcc actgctcaag atcgttcatc ggctggtccg cgagcagctg ctcggcgcgg
    24601 tcgacgatgt acgtgttttc cgcgtggatg cagagcaccg ggtccgccgg cagggccttc
    24661 agccgctgca tggcgtcaag gataaagccg tcgtcctgat ggggatagat ccccttcacg
    24721 ccgcacatat acaccttaaa ggagttcatg ccgcgcgccg aatagagcgg cagctcccgg
    24781 agctgctcat cgtcccgcag cgtaaagtgc ggaatgatgt tgatccgcga atactgcggg
    24841 atctcgcgcg agagcttggc gatcgtcggc agatagctgc ccgtgtggtt gaaaaacgtt
    24901 ccgatcgtgg tcacgccgcc gagcagcgcc gagcgcgttt ccgtttccag ctccgtttcc
    24961 agcggcgcat agagccccag atgggtgtga gggtcgatga tgccggggaa gacgtacttt
    25021 ccgcgggcgt caataacacg gcgggccgac gggcactcgc tggtgagcgc cgcgaccttt
    25081 ccgtttctca cgcagacgtt ggtctgcacc cgtccggact ccggcagaac cacgatcccg
    25141 ttgataatga caagatcgta atccgccccg tcgcctgtac cgggaaactc ggaaacaccc
    25201 agagagggtg cggcgcttac cggcgcggcg gagaccgccg ctgccggtgc gccgctcggg
    25261 gcatacgccg ccgcgccgcg cgtgatctgg atcccgtaat gcttcaccat gccccggacg
    25321 acgtcggtca gcttgtcctg ctcgcccaga accagttcct tttttccttc gcggagcagc
    25381 gcctgaatat ccgcagaagt tatgtatttc attccagcga ctcccctatg gcggagcgca
    25441 gcgatttcat atcacgggag atcgcgctgc ccatcagcgc cagctccgaa ttgatgatgg
    25501 tcatgtcaaa gcccttctcg taccagccct gcgccgcctg gaagtttccg gcgaagatac
    25561 cgagccgctt tccggcggcg cgggtctttt cgcgcatcat catcacggcg ttcacgagct
    25621 tcgggttcga gttctggccg aagatgccca tatccatgga gaggtcgccc gggccgatga
    25681 agaacatgtc cacaaagtcc accgcttcct tcaaaatggc gtcgaggttt tccacggcgg
    25741 agaccgtttc cagctgcacg acgatgttgg tggtgcggtt gcggttctgc tggtcggcca
    25801 gcgtgccgat cgtgtcgccg tactcccaca ggcgggaggg accggcgccg cggcggccga
    25861 tgggggggta ctggctgtac tcacgcagcc gcttgatatc gtccaccgta cggatctggg
    25921 ggatcagcag ggcgtccgcg cctatgtcca gcgcgccgct gacgtactcg tagctcagcg
    25981 tcgggacgcg gaccatgccg taggtaacgc gggaggcctg cagaccctgc agcatcttct
    26041 gcagctccgc aagatggata gcgttgtgct cggcgtcgat gcagaggaag tcaaagccca
    26101 gatggccgag atactccgtc atgaccggat gcggcgttcc gacgaacgtg ccgatcaggc
    26161 gcttgttcga acagatcaca tcccggaagg gggagcggat gggcttgctg taatccggtg
    26221 cgcccgcgtt gccgtacccg ccgccggagg attgcgccac cgtcgcggga gcggacggca
    26281 tggaaactgc cccgcccatg ggagccgcac cgccgccgac ggcgagctgc attcccagac
    26341 gcttgagctc atcccgggcg cagtccgtga ggagcggcgc ttcggggagc gtaataactt
    26401 taacgcctct ttgatgatag gcgcggatat cgctctgagt taaaatattc tgcattgcga
    26461 tccgtcaccg ccttaataga gcttttccag gctggcatcc ggctttgcaa tgatgttggt
    26521 gctgacgacc ttgccgatcc ggttcacggc ttcttcggcg gcgttgaccg ccgtgcggac
    26581 cgcaccgatc tcgccgcgga tgatgacggc gatcatcgtc ttgcccgtgt tttcctttcc
    26641 aacgacctcg acctccgcgg ccttcagcgc cacatcggca gcctccacag cgggaacaag
    26701 gccgacgacc tcgatataac cgtaagcgtt catttcgtgc cctccttaat ccgcgatatt
    26761 cagattcaga accttctcgg cgccttcgct gggcagcgcg atcacatgcg cgcagtgcac
    26821 ctggcccacc gagcgggccg cttctgtgcc ggcgtccacc gcagtacgca ccgcagcgat
    26881 atcgcccttc acggccactg taacataacc gccgccgatc acttccgttc cgaccagctc
    26941 gaccttcgcc gccttcgtca tcgcgtcggc agcggcaaca gcgggaacat acccgacaac
    27001 ttcaataaaa ccccaaggct gcatgatcca atctcctttc aatcattccc cgatgccgat
    27061 catctgggta aggcttttgg ccggatgcgc aacggcgagg acggcctgca ggccgcccat
    27121 ctggttggcg accgccgcac cagcgtcaag cgccgtgtga accgcgctga tctcgccctt
    27181 gatgatgatg gtgacgaggc ccgcgccgac ccataccttc ttcacgacct ctacatcggc
    27241 cgccttggcc atcgtgtcgg ccacctgaaa ggcgggcaca tagcccatag tttcaataaa
    27301 acccaaagaa tacatattgg ctctcctttc caaattatcc ttccgagatc atgtcaacga
    27361 tcccgacaat ataaaggtcg atatacccct gcgtgctgcc gtagcgtctg ctcaccagaa
    27421 cctccgagcc ctcaccggcg cccacgagat ccacggccac gacgcgctcg tgcttattgc
    27481 cgtcgagcgt ctcgcagaga agcattttct ttccggccag actatcttcc ttctgctggg
    27541 aaacaacgtt tccgatcact ttgcaaagaa tcacggatta ccctccttct gccgtatgtt
    27601 ttttatcctc aaagccccag ctgcttccgg ttgaagagga actgaggcat tgtgccctcg
    27661 atgaccgcga tcgcctgatc gaccatgtgg cagcgcagcg tctcgaacgc cttgctcgag
    27721 taatgcgcgg agtgtggcgt caggaccacg gtgtccatcg tgcgcagcgg atgatccgct
    27781 accagcggct cgctctcgtg cgtatccagt cccgcgccct gcagctgtcc ggcgcggatc
    27841 gcgtcggcga gagcgtaggt atcgatcagc gcgccgcggg acacgttgac gatgaaagag
    27901 cccttcttca tcttgcggaa ggcctcggcg tcaaacatgt gccgggtctg ctccgtcaga
    27961 tggcagtgga tggagatcac gtccgactgc tcgagcagct cgtcaaagct gaccttctcc
    28021 gcgccgcgcg cggcgatggt ctctgcgtcg aggaacgggt cgtacgcaat gacgcggcag
    28081 tggaacggcg cgaggaagcg gagcaggtac cgggcgatgt tgccgcagcc ggcaaggcca
    28141 acggtgatgc ccgtcagatt tctcgcttcg tacccggcgc tccatgtcca ctcgccgccg
    28201 cgcactgagc ggtcgagcaa cgtcgttttg cggaacaggt caagaatcag cgcgaacgca
    28261 tgggtggcaa cctcttcgag gcagtagccc gtgtcgttgc acaccttcac gcccttttct
    28321 gtggccgccg gaacgtcaat ggaatccacg ccgatgccga agcggcaaag tcccttcaac
    28381 ttcggcaggc cgtcgatgac ccgcttcgta tacggtgccg ccacaaggat gtgcacatcg
    28441 cagtcctgca tctgctcgag aatgtcgtcc tcggtattgc agttgtgcac ctcaaactcg
    28501 atcccagcct tccggaaccg ctctttttca tacgcaaagt cgatcccaag gcggaaggcg
    28561 tccgtaaatc caaccttaaa gcccataaaa cttcctccgt tctatctgcg aaatttcata
    28621 tgacgctctt cgctcagccc gggagcaggc agagggagac cgccggaata tacgtaacaa
    28681 tcagcaccgc cacgatcatc aagagcatcg gtatcacgat cttcttggag atatcgacca
    28741 ctgagagacc cgtgagaccg ctggccacat agaggttcat gccgatgggc ggcgtgatga
    28801 atccgaccgc gaggttgacg gtcatcaggc agccgaactg gatgccgctc atgccaaggc
    28861 tcgtcataat cggatagagc accggcgcga agatcaaaat cgcggccacg gtgtccatca
    28921 gcgcgccgac gaccagcagg aacaggttga cgagcagaaa gacgacaatc ttgttggagc
    28981 tgatgctgag gatcagattg gcgatgatct cgggcgcacg ctcgaccgtg agtatcttgc
    29041 ccagaac
//
LOCUS       DAWAAE010000007        28227 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_728_length_28227_cov_4.187136, whole genome shotgun sequence.
ACCESSION   DAWAAE010000007 DAWAAE010000000
VERSION     DAWAAE010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 28227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28227
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_28227_cov_4.187136"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3741..3750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5795 a         8693 c         8660 g         5069 t
ORIGIN      
        1 cggaaagcgc cgccgaggag gattcgagcg ccgccttgaa tcccagcagc cgcagaccga
       61 agcccgcggt gaagagaagc gccgcgctct cgccggtgat acggccgatg gagaggatgc
      121 agccggtcac gataccgtct atggcgttcg gcagcacgac ggtgcgcacc atgtgccact
      181 tgccgctgcc aagcgcgaga gcgccctcgc ggtaagctct cggcacggcg agcaggcttt
      241 cgcgcgtgtt cgccatgacc gtggggagca ccatgagcgc gagcgtgagg ccgccggcaa
      301 gaacgccctg cttcagcccg ccgagcttga tgaaaaacag catgccgatg agaccgaaga
      361 ggatggacgg gatacccgtg agcgtttcgg cggcaaagct gatgagccgc acgacgcggg
      421 gattttccgc gtattcggtg atgtacaccg ccgcgccgac gccgagcgga agcgccgcgc
      481 ccatggcgat gagaatgaga tagagcgtgt tgcagatgtt ggggaggatg ccggtcgttc
      541 cggcgagata gctcgtcgcc gtcgtgagga acgaccagct cagctgcggc agaccgcgct
      601 ttaaaatgat cccgatcagg aagagaagaa taccgcctgt tatcaccgcc gcggcgataa
      661 gggctgcttt cagaaaaatt tcccggagct ttctcatgcc ttggcgctcc tttcgctgag
      721 aagccgcagc gcggcgttga tgagaagaat gaagaggaac agcaccagac ccacggagaa
      781 gagcgcgtcg cgctgcaggg agccgtaggc ggcgtaggac atttcggaga cgatggcggt
      841 cgtgagaaac cggacggagg caaagagcga cggcatattc gccacgttgc ccgccaccat
      901 cagtatcgcc atcgcttcgc cgacggcgcg acccgtgccc tggataacgg aggcgatcac
      961 gccgcttttc gccgccctga gcgaaacgag aaaccaggtt tccgccttcg ttgcgccgag
     1021 cgcaagagag gcttcctcat attcgcgcgg cacggcgcgc agcgccgttt ccgaaacgga
     1081 cacaatgtac ggcaggatca tcaccgtcag cacgagaatg gccgtgagca ggcacgcgcc
     1141 agaggagaga gagaacgttt tctgcacaaa cggcacaagc accgtcatgc ccacgagacc
     1201 atacacgacc gaggggatgc ccgcgagcag cgccacgcag ctgcgcgccg cggcggcaat
     1261 gcgcggcggg gcggccttgg cgagaaatac ggccgtaaac aatccgagcg gcacgccgag
     1321 gagcagcgcg cccgccgtgc cgtaaatgct cgtgaggatg aacggcaata taccgaattc
     1381 attcgtcggc gcgttccacg tctcgccgaa aagaaaattc caaagtccga tctcccggat
     1441 cgccggtacg ccggagatca caagatacag gcagatgcac agcacgaatc ccaccgccgc
     1501 gatgccgcag agaaggaaga tccattttac gatattttcc aaaacctttt tcttcataaa
     1561 tgagaaaaac tccccgacgt tcttgtcggg gagtattcta cgttaagggt gtaaaatccg
     1621 ttaccgcggc gttgtaaaat gcgtgtaaag cgcccttttc ggtatcggtg ccgggtttaa
     1681 tgcttccgga acggatcggt atcgtcgtac tggctcggct cgccggtata ggggttatag
     1741 gggacgtggt tgccgtcgtc gtcgatgtag tacatatgca gcgcgtcgcg catctcgaaa
     1801 tagatctccg cttcgcgcca gcggttttgc tcccactcgg gcgagttgtg gatgacgatc
     1861 tgcgtattgg gcagggagtc atacagacgc tgcatctgct cttcattgta catgttcccg
     1921 ccgagataca gccgcttgag ccacgtcatt ttgcagagcg tctgcacgtc cgcctcggcg
     1981 acctcgtcgc tcttgacgcg cttatacatg agatcgatat ccgtgaggct cgtacactcg
     2041 gagagcatgg tgatatccgt gaacgacgcc ttgcccaact ccagaaaccg cagctccttg
     2101 agcgttccga gctccgggat gggatttggc ccggcgctcg agagaataag atactgcaga
     2161 tgcggcatct gcgtgagaaa ataggtatcg ccgtacaggt gcatatgtcc gagatcgacg
     2221 gcgatcatgt cgtgacagta gcgaagatcg tccgcgagat gatcgtactc gccgtaaaaa
     2281 tactcgcagt tataaatgga gaagtacgtc tgatcggtgc gcacgccgta ggcatagacc
     2341 tgcaccatcc agacgaactg cacatcctcg tgccggagat tgatctcctc catattctca
     2401 ttggaaatgc cgcagcgcag catcacgacc tttttgagat tcggcatata ggggagcatg
     2461 tcctcgatct gcgcggcgtc ctccacggtg agcgggatgt ccgtgaactc gatctcctcg
     2521 gcgtcagtcg tgaacgtgac gccgaaaagc tgcgtttccc agatcgtcag aatctccgga
     2581 tgcgccgcgg agaaggtacg cagcgcgtcc tccgaaagtc cgcaggcagt gagcgtaacg
     2641 ctctttgcat cgggcagaag cacgagcgcc gcgccgattt cggagaggtc ttcttccgtg
     2701 agcggcgtgc cggtaaagtc gagcgccgag gcgtccgaga cgaaggtctt gccggcaagc
     2761 gtcacgtccc agacaaagct cacggaggga aatttctcct tcagcgcgag ctggctttcc
     2821 aaagaaaacg tgcttccggt aagcgtcagc gttttgagct ccggcagacg gacgagagcc
     2881 gcgcgcagct cctcctccga agcgttgggg acgataatgt tctcgctgtc gccgtcaaag
     2941 ctctctccgg cgagcgggac ggtccagaac acacgcacct ccggcagcgc agcgcgcagc
     3001 gcgagaatgg cggaaatatc gctgcacgcg ctcacatcgg cgctcgcgag attttcaagt
     3061 ctggcaaagg cagggatatc cccggcagtg aaatccgccg tcacgatgtt ctcgctctcg
     3121 ccgtcgaacg ttttgccgcc aatggcgatg tcccagagaa tgtgcgcgtc cggcagcttt
     3181 ttgcgtgctt tcgtaagctg tttggcggtg atattctttc cgcgcaggtc gattgtttcg
     3241 gcgcggcggg agaccagctt cccgctcacc agcgtataat tcaccccgac gaacacacct
     3301 gccgtgacaa gcagcacgag cgccgctgca aggatcccga tccaagcttt tttcttcatg
     3361 gcatttcctt tcgaaaaggc tccccttaca caggggagtc ttttttttaa ttatactgac
     3421 cgtaccggga aaagtcaatt ttccggtttt ctttttatgt tgtattactt tgtatatctt
     3481 aattaatcgt atttacatta ttaatatata tgtttacacg tccgggaaaa agtggtatac
     3541 ttttataata gctgataata gcgcccgaag cgcgcaggta ataaggagag aaagaatgaa
     3601 ttccctcaac gacatatgga acagcgttct tgccgttctc gggcagaatc tgaccgccac
     3661 ggcgctgaac acctggtttg acgactgcac gccggtcgag atcgtgaata accggctcgt
     3721 catccacacc cccacggagt nnnnnnnnnn acattatcca gtcccgcttt gcccagacga
     3781 tcatcgatgc gctcagcgac cttttctccg ccgagttcaa gctgacgatc ctcgccgggg
     3841 acgagctgga cagctacatc tccgaaacga aaacggacga tacgctcccg gaggtcgcgg
     3901 gcttcacgtt cgataacttc gtcgtgggac cgtcgaacaa atacgcccac gccgcggcca
     3961 tcgccgtggc ggaaaacccc ggcaagctct ataacccgct tttcatttac ggcaactccg
     4021 ggctcggcaa aacgcacctt cttctcgcca tcggcagcgc gatccacgcg aaaaatccaa
     4081 aggcgaagat cgtatacgtc aagggcgacg atttcacgaa cgatcttgtc cacgccatcc
     4141 aggtcggcgg tggcgaggat ctgcgcgcca aataccgcag cgccgatctt ctcctgatgg
     4201 acgatatcca gttcatcgcc ggcaagcagc agacgcagga ggagttcttc cacacgttca
     4261 acacgctcta tgaagcggga aagcagatcg tcgtcacgtc cgaccgcccg ccgatggaga
     4321 tgaacaagct ggaagaccgt atccgcacgc ggctcgaatg gggccttatg gcggacgtgc
     4381 agccgccgga tctcgaaacg cgcatggcca tcacgcgcaa caaggctgcg cagttcggcg
     4441 tcgtgctgcc ggacgaagtc gtaacgttca tcggcgagac ggtcacatcg aacatccgcc
     4501 agcttgaggg cgtggtaaaa aagctcaccg cctaccgcga tcttctggag gacgatatca
     4561 ccgtttcaag cgtccgccgc gccatcaagg acgtgatccg cgtcggaacc tacgtcccca
     4621 cgccgaacgt catcatcgcg gaaacggcgc ggtacttctc cgtttcggag gaggcgctgc
     4681 gcgggcagtc ccgccagaag aacaccgcca cggcgcggca ggtcgccatg tatctctgcc
     4741 ggaatctcac gaacatggcg ctcaccgata tcggcgcgca gtttgaaggc cgaaaccacg
     4801 ccacggtgct cgcctcgatc cgcaagatcg aagacatgat gcaggccagc caggagtttg
     4861 ccatggtcat ccgcgacgtt acctacaaca tcaattccaa ggcataacag caaaaataaa
     4921 gttttccaca agatgtatgt ggaaaaactg tttataaatg ttcaaaactt tttcgttccg
     4981 tcccgatgtg gaaactgtgg aaaattaccc ctgaagtttt ccacatccaa aatccttgat
     5041 tttcaaggct ttcgcggagt tttccacaag tttttgttct actgatactc gcttctaaaa
     5101 aatattctat ctttctttct ggaagaaacg gaggaatcgc catgaaattc acctgtgaga
     5161 aatatctgct cagtctcgcc gtcgccaccg cgggccgcgc cgccgcttcc aaaagcccga
     5221 tcccggcaat ggagggcctg ctcatcgagg ccggacacaa cgtccgcgtc acgggctatg
     5281 atctcaaaaa gggcatttat acaacgtttg aggcagacgt gtccgcgcct ggcagcgtgg
     5341 tgatcaatgc caagctcttc ggcgacatcg tgcgcaagct gccggacggc atcgtttcgg
     5401 tctgctcgga cgagaataac gccgtgaaca tcgcctgcgg aaacgccgat tacaatattt
     5461 ccggcacggc gtcggacgat ttccccgacc ttccgtcgct cgactactgc agcaatctct
     5521 ccctgccgca ggatacgatg gcgcgcatga tcgccgagac gagcttcgcc atctccacga
     5581 acgagtcccg cccggtgtac accggcgcgc tcatcgaggt cgatgacaac actatgacca
     5641 tggtagccgt agacggctac cgcatggcgc tccgccgcga gccggtggaa acatgcgatg
     5701 tggaaagtct caaattcatc gtgccgggcg ctgctctctc cgatctcgag aagatctgca
     5761 tgagtcccga ggagccggtg aagatcgccg tcggcagcaa gcacgtttcg ttctccgtgg
     5821 gagatacggt gctcatttcc cgccgtctgg aaggcgaatt tctgaacttc cgcaagaccg
     5881 ttcccggcga ttttcccatc cagctcaagg cgcagcgcgc cgatctcatc cgctgcacgg
     5941 accgcgtgag cctcatcatc gacgaccgca cgaaaaaccc gctccggtgc gtgttcggag
     6001 aaaacatgct cactatcacc tgcgccacgc cgctcggccg cgcggaggac tgctgcccgc
     6061 tcgagggcga cggcggcggc atgatcgtcg gcttcaacaa cagctatctc atggacgctt
     6121 tcaaggccgc gcccgccgac acgctgcgca tcaacattgt gaacggcagc gccccctgca
     6181 tcatcacgcc ggaggacggc ggcgacagct tcctttacat gatcctgccc gtgcgtctgc
     6241 gcgcggaagg ctgatgcgcg tcgatgcggt gcagacggaa aattttcgtc tgctgcaggg
     6301 cggccggtgc gaattttcgc cgggcgtgaa cgtcatcgcg ggcgcgaacg cgcagggaaa
     6361 aacgacgctt ctcgaagcgg tatatatgct caccggcgcg aagagcttcc gcacgtatta
     6421 cgataaggag ctcatcgcct ttgaaaagga cgaggcggcg gtgcgcggag agtatttcgc
     6481 gtccgagaga gagcagcgca tggagatatt gctccgccgc ggccggacgc gcacaatgaa
     6541 gcgcaacggc gtgaagctct cggcggggga gacggagcag tgcctgaaag caatcctctt
     6601 ctccccggac gatctcgcca tgatccgcgg cgcggcgtcg ctccggcgga ggatgctcga
     6661 tgccgccatc acgcagctcc gccccggcta cggcgcgctc atcgccgaat accggcgcct
     6721 gcaggaaaat aaaacgcgca ttctgcgcga ctggcgggaa aagccctcgc ttctcgatac
     6781 gctggacgta ttctcggacg gaatgtgtat gtgctcggcg aagatcatcc gttaccgcgc
     6841 gtcgtttgca agacggctcg ccgagaccgc gcgaagcgtg cagagcgaat tttcccgcgg
     6901 cacggaagat ctcacgctca cctacaccac cgtttcaacc gtggaagacc ccgcggcggc
     6961 ggagcgggag atatacgagg cggtctccga acggcagcgc cagctgcgcg gcgcggagat
     7021 cgagagcggt ctctgtcttg tcggcgcgca taaggacgat cttctgatca ccatcaacgg
     7081 cgcggacgcg cggagctatg cctcccaggg tcagacacgc acggcggcgc tctccttgaa
     7141 gctcgccgag cgggagatat ttctcgccga gaccggcgag acgccagtgc tgctgctcga
     7201 cgacgttttg tccgagctcg acagcgcccg gcaggagttt gttttgaacc gcatcggcgg
     7261 cggacagacg ctcgtgagct gctgcgaggc ggcgagcgta gagcgcatga cgggcggtcg
     7321 cgtactttat atggacaaag gacgggtaaa cgaagtatgt acctccattt aggcgcggaa
     7381 acggtcgtgc ggaccgacag catcgtcgcc gtgtgcgatc tcgataacgc cagctcctcg
     7441 catatcacgc gggaagctct ccgcgccgcg gagaaggcgg gcaaggtcgt gaacgtggcg
     7501 gacgatctcc ccaaatcgtt cgtcgtctgc cgggaggacg gaaagacccg gatctatcta
     7561 tcgcagctca acaccgcgac gcttttgaag cgcgcggaga atatatcctt tgattaaact
     7621 catggaggaa cacccatgtc ggaaaataca ccagtcaatc ccaattccta tgacgccagt
     7681 cagatccagg tccttgaagg tctggaagcg gtgcgtatgc gcccgggcat gtacatcggc
     7741 tcgacgagca cgagcggtct gcatcacctt gtatacgaga tcgtggataa cgccatcgac
     7801 gaggcgctcg cgggcttctg cacgcatatc gaggtctcca tcgaagacgg cgagagcatc
     7861 tgtgtgaccg ataacggccg cggcatcccc gtggacattc aggagcagac gggcaagcca
     7921 gcgctcgagg tcgtctacac ggtgctgcac gcgggcggca agttcggcgg cgggggctac
     7981 aaggtctccg gcggtctgca cggcgtcggc gcgagcgtgg tgaacgcgct ctcggactgg
     8041 cttgaagtgc gcgtattcaa aaacggcaat atctatgaga tgaaattctc ccgcggccat
     8101 atcacgcagg agatgaagat cgtcggcaag accgacaaac acggcacgca ggtgcgcttc
     8161 catcccgatc ccgagatgtt tgacgacacg gtctataact atggcgtgct cttcaagcgc
     8221 atgcgtgagc aggcgtttct gaacgccggt ctcaccatca tcatggagga caagcgcccc
     8281 gagcaggagc agcggcagga gctgtgctac gagggcggca tccgcgagtt tgtcaccgag
     8341 ctcaaccgcc acaaaacgcc ggtgcacgag aacgtcattt atatgtccgg cgagcgggac
     8401 acctccatgt gcgagatcgc catgcagtgg aacgacggct acgatgagcg catcgagtcc
     8461 ttcgccaaca acattctcac gcccgagggc ggcatgcacg agaccggctt ccgcacggcg
     8521 ctcacccgcg tgatcaacgc ctacggcaaa tcgagaaaga ttttgaagga cgaggagacg
     8581 ctctccggcg aggactgccg cgagggtctc accgccgtca tctccgtaaa gctcgaaaac
     8641 ccgcagtttg agggccagac aaaatccaag ctcggcaaca gcgacatccg cacgatggtg
     8701 gacagcgttg tgagcgacaa actcgagcag ttctttgagg aaaaccccgc tgttgccaag
     8761 cagatcttgg aaaaaggcat ttccgcctcc cgcgcgcgcg aggccgcgcg caaggcgcgc
     8821 gacagcgtcc gccgcaagac cgggctggag tccggccaga tgccggataa gctgcaggac
     8881 tgcaacgagc gcgacccgtc cctctgcgaa atttacatcg tcgagggcga cagcgccgcg
     8941 ggcagcgcca ttcaggggcg cgactcccgc ttccaggcca ttctcgccat gtggggcaag
     9001 atgctcaacg ttgagaaggc gcgcgtcgat aagatctacg gcaacgacaa gctctatccg
     9061 ctgatcgttg cgctcggcgc gggcatcggc aatgagttca acatcgataa gctccgctat
     9121 cacaagatcg tcatcatggc cgatgccgat gtggacggct cgcacatccg gacgcttttg
     9181 ctcacgttct tcttccggta tatgcggccg ctcatcgaaa acggctatgt ctattccgcc
     9241 gtgccgccgc tcttcaagct gagccgcggc aagcagcagc gcgtggcgta ctccgacgaa
     9301 gagcgcgacg caatctcccg cgagctgcgc ggcgacaacc cggacgcgaa ggtggacatc
     9361 tcccgcttca aaggtctcgg cgagatggat ccgcacgagc tctgggaaac gacgatggac
     9421 cccgagcgcc ggacgctccg ccgcatcacg ctcgaggacg cgcagcgcgc cgatgagata
     9481 tttaccgttc tcatgggcga cgaggtcgag ccgcgccgcg cgtggatcga gaccaaggcg
     9541 cgctacgtcg tcaatctgga ttattaagga ggcggcataa tggcacacaa acgcgattat
     9601 aagccggaag atattgcctt ccccgaacag catatcgttt cctccgagct cgtctcggaa
     9661 atggaaaaga gctatattga atacgccatg tccgtcatcg tcgggcgcgc tctgcccgat
     9721 gtgcgcgatg ggctcaagcc cgtccaccgc cgcattctgt acgcgatgta tgaggacggc
     9781 ctcacgcgcg ataagcccta ccgcaagtgc gccaccgccg tcggcgacgt gctcggccgg
     9841 tatcaccccc acggcgacgc ttcggtgtac gacgcgctcg tccgcatggc gcaggacttt
     9901 tcgctgcgct acccgctcat cgacggccac ggcaacttcg gcgacgtgga cggcaacccc
     9961 ccggcggcct accgttacac cgaggcgcgt ctcgcccgca tggcggacga aatgctccgc
    10021 gacatcgaca aggaaaccgt agactgggac cccaactttg acgagacgag aaaagagccg
    10081 cgcgtgctcc cgagccgcat tccgcagctt ctcgtgaacg gctcctccgg tatcgccgtc
    10141 ggcatggcga cgaacatccc gccgcacaac ctgcgcgagg tcatcggcgc ggcggtcgag
    10201 gtgctcgata acccggacgc gacgctcgac gatctcatgc gccacgtcca cggccccgac
    10261 ttcccgacga agggcatcat catgggccgc gccggtatcc gcgccgccta cgccacgggc
    10321 cgcggcaagg tcaccatccg cgcgcgcacc gagtttgaag agcacggaca gaaccgtgtc
    10381 cgcatcatcg tcaacgagct gccgtatcag gtcaacaaga agatgctcat caagaacatc
    10441 gccgagcagg tcaaggataa gcggctcgac gatatttccg atctgcgcga cgagtccgac
    10501 cgcaacggca tgcgcatcgt catcgagctc aagcgcgacg ccaaccccca ggtcgcgctc
    10561 aacaagctct ttgcccagac gcagctgcaa agcacgtttt ccatcatcaa tctggcgctc
    10621 gtggataacc agcgccagcc gaagatcctc tccctgcgcc acattctcga cgagtatctg
    10681 acgttccagg aagaagtcat cacgcgccgc acaaagtacg atctccgcaa ggcgcgcgag
    10741 cgcgcccatc tgctcgaagg actgctcatc gcgcaggaca acatcgacga ggtcatccat
    10801 atcatccgca cgagctacga taacgccaag gaaaacctta tggcgcgctt cgggctggac
    10861 gatgtgcagg cgcaggccat tctcgacatg cgtctgaagg cgctgcaggg actggaccgc
    10921 gagaaactgc agaatgaata tgacgagctg gaaaagaaga tcgcgtatct cgtctccctg
    10981 ctcgaggatc ccgaaaagct caagggcgtt ctgaaggacg agcttctcgc cattgccgac
    11041 aagtacggcg atgaccgcaa gaccgaaatt caggatgtgg aagacgagat cgacatggaa
    11101 gatctcatcg ccgaggaaga gtgcgtcttc acgctttcgc acaacggcta catcaagcgt
    11161 acccccgccg ccgagtaccg cgcgcagagc cgcggcggaa agggcgtgcg cggcatggcc
    11221 acgcgcgagg aggactatgt ggagagcgta ttcaccgcct cgacgcacga ttttatcctc
    11281 ttcttctcga gccgcgggca ggtgtacgtc cgcaagggct accagatccc cgaggccggg
    11341 cgcagcgcgc gcggcacgaa catcgtcaac atcctgcctc tcgagccgga ggagaagatc
    11401 tccgcgatgc tccgcggcaa gggcctgcag gaggacgatt tcatcatgtt cttcacgaag
    11461 aacggcgtca cgaagcgcat gtcgcaggga gagctgcgca atgtccgcaa aaacggcatc
    11521 cgcgcgctcg acatccgcga gggcgacgag ctcgtacagg cgctcacgac gcacggcagc
    11581 gagaagctgc tcgtcgtaac gcgcggcggc atggccatcc ggttcgacga aaacgacgtc
    11641 cgtccgatgg gccgcacggc cgccggtgtg cgcgccatcc gtcttaagga cggcgacgag
    11701 gtcgtcgcgg cggtgcgcgc cacggacgag gacgcggcgg tgctcaccgt taccgagaac
    11761 ggctacggca agcgcacccg catcggcgaa tacagcgtgc agaaccgcgg cggtcagggt
    11821 ctcaagactt accagatcac gcagaaaacc ggcgagattg tcggcgcgaa gaaggtcacc
    11881 ggcgaggagg atatcctgct cgtatcggac gacggcacga tcatccgcat ggaggccggc
    11941 agcatctcgc ttctcggccg cgccacgcag ggcgtgcggc tcatgcgccc ggcggagggc
    12001 gcgcacgtcg tcgccatggc gcgcaccgag cgcgaagcaa gcgaggaaga gaccgccgaa
    12061 ccgggcgaga gcgcggtaga gatcacggag gaataaatgg aagctcgttc tctttttatc
    12121 atcgccgtgc tgatccttgc ggcgctggta aaagcttcgg tcaaaaatac cgcgcgccgg
    12181 ggcggtagaa cgaccggaaa accgatcgga aagaacggcg agcggatggc gcaggcgcag
    12241 gacgcgccgc agcgcagcgt tcctccgcgg cggtctatgc cgccggatat gccggacgcg
    12301 gcgtgcgtcg tatgcgacaa taccggcgag gatcactttg cccacgacaa ggcgcagcgc
    12361 atcgcgcagc tcgacgattt tctcaaaaac ggcatcatcg acaaggcgga gtaccgcacg
    12421 ctgcgcgagc gctacgagcg cggactataa gccataagag agaaaaccat tcaaaccatt
    12481 cagggagaaa tatgaagaat atcattttgt ataccgacgg ggcatgctcc ggcaatcccg
    12541 gccccggcgg ctgggcgtcc atcctgcgct atggagacag catcaaggag ctctccggcg
    12601 gcgaaaagga cacgacgaac aaccgcatgg agctcacggg cgtcatcgaa gggctcaagg
    12661 cgctcaaaga gccgtgcgcc gtggagcttt attccgacag tcagtatatc gtgaacgcct
    12721 tgaataacgg gtggctgcgc gactggaaaa agcgcggctg gaagcggcgc gacggcgagc
    12781 tcaaaaacgc cgagatgtgg caggagctgg atgggcttct gcagaagcac cgcgtcaccg
    12841 ccgtgtgggt gcgcggccat gccgacaacg agtggaacaa ccgctgcgac gcgctcgccg
    12901 tcgcggagcg cgaaaagctc gcgtgaaggc cgtgaaaaaa aagaaagact ttacccgggg
    12961 cagtgtgcgg gtattctttt cctactatac cccgcaccgg aagctgctgc tgcttgatct
    13021 cgtgatggcg gtctttgcgg tagcggcgga gctggtgttc ccgtacgcca cgcgctacgc
    13081 gctcaaccgc ctgctgccga acggcctcta caaagccttt ttcctgctga tggcgggact
    13141 tctcggcgcg tatctgctgc gcgctctcgc gcagtatatc gtgaccgtgt acggccatga
    13201 gctcggcgtg cgcgtcgagg cggacatgcg cgaggacatt tttgccaagg tgcagacgct
    13261 ctcgttcgcc tttttcgata agcaccgcac cggcaagctc atgtcccgca tgacgaccga
    13321 ccttttcgac atcaccgagc ttgctcacca cggcccggag accgtgatcc aggcctcgct
    13381 cacgatcgcc ggggcgatcg tcatccttgc cacgatccgc tgggagctgg cgctgatatt
    13441 gtttcttctg ctgccggtgc tgctcgcggt cgtgatgcgg ctgcggctgc ggctccgggc
    13501 gcgcagtctg gacgtgaagc gcgagaccgc cgagatcaac accgctgtgg aggcgggcgt
    13561 ttccggcatc cgcaccgtca aggcgtttgc caacgagcag gacgccatgg acaagttccg
    13621 cacgtccaac gagaaattca aaaaggtcaa aaaaagctat taccgcattt tcggcgtgtt
    13681 caacgcctcg accgagtttg ccatggcgct catgcagctc gtaacgctcg ccgccggcgg
    13741 agtgttcatt atgcagggca aaatggacgc ggtcgatctc atcacgtttt cgctgtacgt
    13801 ttcggtgttc atggcgccga tccggaagct cacgcagttc gttgagcagt tccagaaggg
    13861 aagttccggc ttcctgcggt ttctggagat catgcgcgcc gagccggacg tgaaggacgc
    13921 gccggacgcg aaggagcttg aaaacgtaaa aggcggcgtg gagtaccggg acgtgtcgtt
    13981 ccactatcag aacggcgaag aggtgctgcg ccatgtgaat ctcacgatcg cgccgggcga
    14041 aacgctcgcg ctcgtgggcc cgtcgggaag cggcaagacg acgctatctc agctcctgcc
    14101 gcggttttac gatgtgaccg gcggcgcggt tttcgtagac gggcaggacg tgcggaaggt
    14161 gacgcaggcc tcgctccacc ggtacatcgg catcatacag caggacgtgt tcctctttgc
    14221 cgacaccgtg cgcgagaaca tccgctacgg ccgccccggt gcgacggacg aggaggtcgc
    14281 gcaggcggcg atcctcgcgt ccatccacga ggagatcctg gaaatgccgc acgggtacga
    14341 tacgttcgtc ggcgagcgcg gcgcgatgct ctccggcggg cagaagcagc gcatcgccat
    14401 tgcgcgcgtg tttctgaaaa acccgccgat cctcgtgctt gacgaggcga cgagcgcgct
    14461 cgacagcgtg accgaggccg cgatccaggc atcgctcgaa aagctcagcc gcgggcgcac
    14521 gacgatcgtc gtggcgcatc ggcttgccac ggtccgcggc gcggatcaca tcgccgtcat
    14581 cgacaaagaa ggcgtgagcg aatacggcac gcatcaggag ctcatggaga aaaacgggct
    14641 ctacgccgcg ctctggaata cgcagaaact gtcgtgaaaa tgagggcgga gcagagcccg
    14701 cccctgcgga ttgctttaaa aatacctgta gtggcggggc tctgctccgc cataaactcc
    14761 gcacttgcaa aagttatacc gtccgcctat aatacagggc agatgatccg ggaggaatga
    14821 actatgaaac gaacgaagat caaagagctg tggacaaacg ccgccgcgtt tgacggagcg
    14881 cacatcaccg tctgcggctg ggcgcgcacg atccgcgata tgaagaattt cggctttctc
    14941 gagctgaacg acggcagctc cttcaaggga gtgcaggtcg ttctcgagcg tgagaaggtc
    15001 gccaattacg acgagatcgt ccgccagaac gtcggcgcgg cgttcattgc cgagggcacg
    15061 ctcgtcctca cgccggacgc tccccagccg ttcgagctca aggcggatac cgtcacggtc
    15121 gagggcgttt cctcgccgga ctacccgctg cagaaaaagc gccacagcgt cgagtatctg
    15181 cgcacgatcc agcatctccg gccgcgcacg aatctgttct ccgcggcgtt ccgcgtgcgc
    15241 agcgttgccg ccgccgccgt gcatgagttt ttccagagcc gcggctttgt gtacgtgaac
    15301 acccccatca tcaccggctc tgactgcgag ggcgccggcg agatgttcca ggtcaccacg
    15361 ctcgatatcg ataacccgcc gcgcacgccg gacggaaagg tcgattactc caaggacttc
    15421 ttcggcaagc gcacgagcct cacggtctcc ggccagctca acgccgaaaa cttcgccatg
    15481 gcgttcggcg acgtgtacac cttcgggccg acgttccgcg ccgaaaactc caacacccag
    15541 cgccacgccg ccgagttctg gatgatcgag ccggaaatgg cgttctgcga tctggacggc
    15601 gatctggtcg tcatggagga aatggtgaag tacatcatct ccaccgtgct tgaaaagtgc
    15661 ccgcaggaga tggatttctt cacgaaattc gtcgataagg gcctgcgcga gcggctcgag
    15721 cacgtgcgcg acagcgcctt cggccgcgtc acctataccg aggccgtcga gcttctgcag
    15781 aagtccggca aggaatttga ctaccccgtt tcctggggcg tcgatctcca gaccgagcac
    15841 gagcgctatc tcaccgagga aattttcagg aagcccctct tcgtcaccga ttacccgaag
    15901 gagatcaagg cgttctacat gcgcatgaac gacgacggca agaccgtcgc cgcggcggac
    15961 tgcctggtgc ccggcatcgg cgagatcatc ggcggctcgc agcgcgagga gcggcttgac
    16021 ctgctcgccg cgcgcatggc cgagctcggc ctcaacgagg aggattactg gtggtatctc
    16081 gatctgcgcc gctacggctc ctgccgtcac gcgggcttcg gtctcggctt tgagcgcatg
    16141 gtcatgtacc tgaccggcat ttccaacatc cgcgatgtgg agctgcatcc gcgtaccgtc
    16201 ggcaacgccg atttctaatg aaaaggataa acccctccgt cacggctatg ccgtgccacc
    16261 tcccctgtta ggggaggcaa gttagtcatt tgcaaactat cttggccccc ctaacagggg
    16321 ggctggcagc cgcaaggctg acggaggggt ttcttgtaaa aaaacaaaac cataaggggg
    16381 acacaacatg aagtacaaca ttcaaggcga gccgatgccc gttgtcatct gccatctgga
    16441 agcgggcgag agcatgatca ccgagcgcgg ctccatggtg tggatgagcg ccaacatgcg
    16501 catggagaca aagaccggcg ggcttggaaa ggcgctcggc cggatgttcg gcggtgagag
    16561 ccttttccag aacacctata ccgccgaggg cggccccggc atgatcgcct ttgcctcaag
    16621 cttcccgggc gcgatccgtg ccgtggagat cacgccgaat atgcccgtta tcgcgcagaa
    16681 gagcgcgttc cttgccgcaa cgccgggcgt ggatctttcc atccacttcc agaaaaaagt
    16741 cggcgccggt ttcttcggcg gcgagggctt catcatgcag aagctctccg gccacggcat
    16801 ggcgtttctg gagatcgacg gctcggcggt cgaatatacg ctcgcgccgg gacagcagat
    16861 cattgtggac accggcaatc tcgccatggt ggacgccacg tgccatatcg atatccagac
    16921 cgtccgcggc gcgaagaacg tcctcttcgg cggcgagggc cttttcaaca ccgtcgtcac
    16981 cggtccgggg cgcgtcgtat tgcagacgat ggcgctgccc gccttcgcga gcgcgctcat
    17041 cccgtatctg ccggcgaagt cggactgacg ctcacacagc aaaatggata gaaagcggcg
    17101 ggagaaaacc gccgccttct atccagaacg gatatttttt caaaggtact tgaatttttc
    17161 acaaactaag aatagtatag gcggacacgg agaaaattta cggatttttt tcgtgttctt
    17221 tcttctgtta tatatgtcgg attttatata tgttgtttgt gccgaggtgg gtaaatgaaa
    17281 aaaagatatg agatcaatat gacaaaaggg ccgctcctgc cggagcttct gcagtttatc
    17341 ctgccgctga tgctctcgtc catgctgcag atcgcgttca acgccgccga cctcatcgtc
    17401 gtcggccggt tcggacggcc gcactccatg gcggcggtcg gcgcgaacgg cgcgatgatc
    17461 aatctgatca tcaatgtgct catgggtctt gccacgggcg gcggcgtgct ctgcgcacgg
    17521 tattacggcg cgaaccagac cgacaagctg caccgcaccg tgtccacaac gctcatcacc
    17581 ggcgtgatcg gcggcgtgat caccggcgct ctcggacttg cgctcacggt gccgctgctg
    17641 cggctcgtcg gcagcccgga cgaggtgctg ccgctcgcca cggtgtatct tcggatctac
    17701 ttcctcggcc tgccggtcat ggcgctttat aacttttccg ccgcggcgct gcgcgcgctc
    17761 ggcaacacgc gccagccgct catctatctt gccatcgccg gtgtgctgaa cgttattatg
    17821 aatctctttt tcgttatcgt cgtcggcatt gacgttgcgg gcgttgccat tgccacggtg
    17881 ctcagccagt gcgtttccgg cttcctcacg gtgcgctgcc tgctgagatc gcaggagctg
    17941 cacgttaaag agctgcattt ctccggccac attttcaaga aaatgatgaa tatcggcatc
    18001 cccgccggta tccagggctc gctcttctcg atctccaact ttattattca ggcttcggtc
    18061 aactcgttcg gcgcggcggt catcgccggc aacagcgcgt gcgtgagcgt cgaggggctg
    18121 ctctactgcc cgtcggacgc cgtaatgcag gccgccacca ccgccgtgag ccagaacgtc
    18181 ggcgcgcagg agtatgagcg ctcgcgctcc gtcgcccggt ggtcgatggt ccttgttgtg
    18241 gtcctgaccg gcatactgac ggtgccggcg gtcgtattcc ggcggcaggt gctggggctc
    18301 tatacttcga gcgaggcgga gctggaggcg gcgttcaccc gtatgatgat cgtctgctta
    18361 acgtattcgc tcgacggcac gatggcggcc atgtccggcg tgaaccgcgg tctcgggtac
    18421 agcatgctct ccgcggcggt gacgttcgtc ggcgcgtgcg tgttccgcat catctgggta
    18481 tatacggtgt ttgcgagcgt cggtacgctc gagagcctgt atgtgtccta tccgatctcc
    18541 tggatcctct ccacggcggc gcacatcgtg tgctactaca tcatccgcaa aaagccgttc
    18601 aaggcgacgc tcgaagcaaa agccgccgcg gcttgatccc ttcatcttct caaagaaaag
    18661 accggcgcct cccgtttttc gggaggcgcc ggtcagtctg tcgaaaaagc ctcgcagagt
    18721 ttgcgcccgc aggcgcaaag aaagtttaaa acatttttgc cgatgacatg tgcatcggca
    18781 aaaatacttc tcggctcgca aacgtgcgag cgcaagcgag tgcgctgcgg cgagccgcag
    18841 ctttttcaaa cgagagccca acgaagtggg tctcgtttga gatgtattat caggccgcgg
    18901 ggaggggcat ggaggtgatg ccggggacct cgccggtggc ggcgtccacg tagcagtcgt
    18961 actgcagcca gttgctggtg aagcggactt cgtagtagcc gttatcgagc gtgatctctt
    19021 cgtcggtgat ctcgttggtg tttacacccg cgctgcgggc ggcctcgctc agcgcgacgg
    19081 cggcggtcag attcagttca taaatgttgt tcttcatacg ataaaactcc tttgcaaatt
    19141 ctcccttgtc ggcgggacat ggggtttctt tcatcttggc tgtagtttac aaaggaattc
    19201 taagaaaaat attaaaagaa caaaaagaaa gaaaaatttt tttctttcta atttttgacc
    19261 ggcggcagaa cgatgcggaa gtcgcagccc tttcccggct cgctgacgac ctcggcgcgg
    19321 gcatgatgca cgagcgcgat ctcccgcacg agcgagagcc ccagacccgt gccgccctcg
    19381 gcacgggagg tgtcgcaccg gtaaaaccgc tgccagatct tttcctgctc ggtcttgggg
    19441 atgccggggc cgtcgtcctt cacggagatc acggcgcagc cgttttcccg acggagcgag
    19501 acgcggatgt ttccgccttc gcgcccgtat ttatacgcgt tctgcatcag attgaacatc
    19561 gcccgcgaga ggagcgaggg gttgtacgcg gccgcaacgc cgggctcgat atcggtctcg
    19621 agcgtcatgc cgcgctcgtc cgcggggacg aactcgtcgc agcaggcgtc tacaaagccg
    19681 gagagatcgc acacgcgcag cgggtatttt tccgtgccct gctggatgcg cgtgagcgag
    19741 agcagctgct cgatgagcgt attcatcttg ttgccctgct gctcgatgac gttgatggag
    19801 gcgagaaagt cctccttcgt ttccgccttg cgcctggcgc ggtcacactc ggcgaggatc
    19861 accgccacgg gcgtgcgcag ctcgtgcgac gcgtcggcgg tgaactgctt tttggagagg
    19921 aacgacgttt ccagccgcgc gaacataccg tcgaacgtgc gggcgagccg ccgcatctcg
    19981 cgcgggccgc gccggagatc aagccgggcg gagagatcgt cgccgtcgga aatgctgccc
    20041 gcggcttcgg tgatgcgctc gagcgggagg aacgagtccc gcgcgatgag ccagccgccg
    20101 ccgatcgaga gcagaagcag caccggcagc agcgaccatg tgagcggcag tatgacgcgc
    20161 cccggccccg ccgcggcgtc cgcggaaatg acgccgcgca cccagagccc cgtgatcgtc
    20221 atatcgacat aggaatcgta tacgagccag tccttcccgt ccgcggagta ggggcgcacc
    20281 gcgcccgtct ggagcggcag agcgaccgcc tccgcaaacg gcatagcgcc gcggagatac
    20341 gtccccccgg cgtcatagta ggtgcagtac accccgtgct tgtattcctt catatcgtcc
    20401 cagtcgaacg tgccgttatc aaactcaata tcgtcggcgc gcctggagac ttccttgacg
    20461 acctgcgatg tcgggttcgc gatcaccgag ccgttcatta ccagcacgaa cgtgagcgta
    20521 acgcccgcga gcagaagcat catcagcgtg aaccagagca ccatccgcca ctttgccgac
    20581 atattcaatc ctcccgcagc acccagcccg tgccccacac ggtatgaatg agttttttgg
    20641 aaaacccggt gtcgatcttt ttgcgcaggt agccgacgta tacgtccacg acgttcgtgc
    20701 cgccctcata gtcgtagttc cagagattgt tctcgatgcg ctcgcgcgag agcacgacgc
    20761 ccttgttccg gatcatgtat tcgagcaggg cgtattcgcg gctcgtcagc tcaatgacac
    20821 gcccggcgcg gacgaccgtg tgcgcgctca cgtccacgga gagatcggcg atcgtataaa
    20881 cgttcgtgcg cgtgtcggta tacttgcgcg ccatcacgcg caccaccgcg agcagctcct
    20941 gcatgtggaa cggctttaca agatagtaat ccgcgccggt ctccaacccc tcgaccttgt
    21001 cctcgacgct gtcgcgggac gtgaggaaca gcacgggcgt cgtgctgccg ctctcgcgca
    21061 gcttacgcac gagcgtcagc ccgtccatgc ggggcatcat aatgtcgagc acgagcacgt
    21121 cgtattccgc gcacgcgagc gcgtcccacg cctcctgccc gtcaaagcag ggatcgacgc
    21181 tgtacccctc gtcggtcaga gcctcggtca gaatgcggtt gagatttctc tcatcctccg
    21241 ccacaagaat tcgcatgaca gttcacctcg gagcatagtg taccggaaaa aagtaagaaa
    21301 agtcttaaaa gactaagaag aaaatgcctc cccaagggga ggcgtaatgc aattattccg
    21361 cgtgctgggc ttcggccagg gtgacgatgt cgccgacgcc gtcgagcacc gcgctgggac
    21421 ggtaggcgta ttcgtcgagc gtcgcctccg tggagatgcc ggagaggacg agcaccgtgg
    21481 acatgccgca ctccatgccg gagatgacgt ccgtgtccat tcggtcgccg accatgacgg
    21541 cctcggcgct gtggcagccg agacgcttga gcccggtgcg catcatcagc gggttcggct
    21601 tgccgcagaa gtacgcctgc ttgccggtgg ccatctcgat gggcgcgatg agtgcgcggc
    21661 acgccggggc gatgccgttt tcgatggggc cggagaggtc ggagttcgcg ccgatgagct
    21721 ttgcacccgc aagcacgaga ttcgtcgcct tagtgagcga gtcgagcgag tagttccgcc
    21781 cttcgccgac gatcacatag tcggggttca cgtcgttcat cgtgatgccc gcatcgtaga
    21841 gagcgttcag aaggcccgcc tcgccgatga cgtaggccga gcagcccggc gactggtcgc
    21901 ggatgaacgc cgccgttgca agggcgctcg tataaaaccg cgcctccggc acgtccagcc
    21961 ccatgcgggc gagcttctgc ttgagctcga gcggcgtgca gccgctattg ttcgtgagaa
    22021 agagatattc cttgccggtg cgctgcagcc agtcgataaa ctccggcacg ccggggagaa
    22081 ttttgttgcc gtgatagatc acgccgtcca tatcgcagat aaagcctttt ttgtcattaa
    22141 aattcagcat agtattcctc cttgcaattc gatgtaaata cgccgaaaac ccgcggcgag
    22201 cgggcttcgg acgggatatg ttatttttcc gggaatgtca cggtgaaggt cgagccttcg
    22261 cccggctcgc tctctacgcg cacgtccgcc ttctggatga gcgcggcgtg cttcacgatg
    22321 gagaggccga gccccgtgcc gccgacggcc ttggagcggc tcttgtccac gcggtaaaac
    22381 cgctcgaaaa tgcggctctg gctgtccttc gggatgccga cgccggtgtc gctcacggtg
    22441 aggacgaccg cgccgtcctc cggcgtgacg gcgatatcga cgcgcccgcc ggggcggttg
    22501 tacttgatgg cgttgtcgca gagattgtag atgatgctgt ggagaagctg cggcacgccc
    22561 tgcatcggcg cgctcacgcc cttgagagag agcgtaacat cggcgccgga ggcttccgtt
    22621 tcaagactgt ctacggcgtt ttccgcgatg cggagaagat cgcaggacgc aaatacgtag
    22681 tcctcgccgc ccgcgtcgag ccgggtgaga tcgatgatgt cctcgacgag cttcgtcatg
    22741 cggccggact cggtgcggat ctttccggcg aagggcttca cgtcctccgg ccttgcgagc
    22801 ccgctttcga gcagctccgc gtacccggag atggcgtgca gcggcgtttt cagctcgtgg
    22861 gagacattcg cggtgaactc ctgccggatg agcgaggcgc gctcgatctc ggcgcggtca
    22921 cgccggagct gcgcctgctg cgcggcgatg cgccgcagaa gtggctcgac ctcgcggtac
    22981 tcgtccttct cataatactg catcgggttg tccgggtcaa tggcattgat gggcgcgacg
    23041 attttttttg tgagccgcga ggcgagcagg aacgagagcc caagcgcgag cagcgccaca
    23101 aggcagatcg gctgcgcaaa cccgagcaca agcgaccaga gcgcgagctg cacgaccgag
    23161 agacggagca ccgtcccgtc cggcagacgc tgcgcggcgt aaagctggcg gtcggcaagc
    23221 gtgggggagt agcggcgcgc ctcgcccgtg ccgttctgga gcgcctggcg gatctcctcg
    23281 cgctcgagat ggttttccat gcgcgaggcg tccgccccgg tgtcgaagag caccgtgccg
    23341 tccgcgtcga tccaggtgac gcggtagtcg ccggtcgaaa ggccggtgaa gtagtccttg
    23401 ccgcagagcg acacgccctg cgcggcgagc gccgtttccg cgtggagctg cttcatctgc
    23461 acgccggtaa agtagttata cgatacgccc atgataaaga cgagcgacgc ggcgaacacc
    23521 gcgagcgccg cgccccagat ggcggtgaat attttccggc tcatttcacg ccctccattt
    23581 tatagccgac gccgcgcacc gtttcaatga ggccgccgca ttcggcgagc ttcgtgcgca
    23641 gtgtgccgac gtgcacgtcc accgtgcgcg tctccccggc gtaatcggcg tcccacacgc
    23701 gcgagagcag cgcttcgcgc gagaacacct gcccgatgtt ttcgagaaac agcttcaaaa
    23761 gctcgtattc cttgagcgtg agagaaacgg gacggtcgtt caccgtgacg gcgtggcggc
    23821 cctcgtcgag cgtgacgccg ccgcaggaga gcacgccgga cggctggcgt gccgtgcggc
    23881 gcagtaccgc gcggatgcgc gagaccattt ccatcatgcc gaagggcttg gcgagatagt
    23941 cgtccgcgcc ggtgtccagc ccgacgacct tgtcgtactc gctgccgcgg gccgtggcga
    24001 gaatgacggg aagctgctcc gtgagcgggg aggagcgcag ctttttcaga atttccagcc
    24061 cgtcctcgcc ggggagcatg atgtcgagca gcacaagatc cggcacatgc tcgcacatgg
    24121 cctcccagaa cgcgccgctc tcggcaaagc cccgcgcctc aaagccggac gcgcgcagcg
    24181 tatacagtac cagctcgcgg atgctggcgt cgtcttccac acaatatatc ataaggacgc
    24241 cccctctcat tttcgagcat atcatactat atgaaatgta aagagggaaa ccccgttcgt
    24301 gtaaaatcaa agtaaaatgt aaaaagggcg aagaaaaccc ctccgtcagc cttcggctgc
    24361 cagcgcccct gaaaggggag gcggattttg ccgcaggcaa aagacggaag ggtttccccg
    24421 ccggtacgtt taaattttct ttcgtatcag cccgacgacg aacgccagca cgagcacccc
    24481 ggcggcggag gcgctggcaa tgggcacgag cgtgcgcgac agctcccgca gggaaaacgc
    24541 cgtcggcgcg cacagcgccc cggataggag cgcgagcgca agacatgtga gagcaacggc
    24601 gccgcgccgc gccccgagct tcgggaagca cagcgtgagc tcgcctgttg ccgcctgcaa
    24661 aagcgcgccg agcaggagaa agtcggtcgt gacccactgc gccacgacga gcgcctccac
    24721 gcgctcgagc aggcggaaga tgcggatgca ccggatcata acgaaaaacg gataatacat
    24781 ctcggcggtg agcttcgcgc cgaacgtggc gacgaccgtg acgcataaaa gcgccgcgag
    24841 cagaccgagc gcgagaaacg cgcggttgaa cggcgcggcg agcggccccg gctccgtccg
    24901 gtcggagagg aacgcgagca gaagcagcac gctcatcgtg ctcaaaagcc acggcgcgcc
    24961 gcgcgcggca tcggcgagag aatcgctcga tatcggcgcg aggtttgccg cgtccagccc
    25021 cggcaggcag aagaggaacg cgaacagaaa tacgagcgca agcagcggca gcgcgatctc
    25081 cgcgcagcgt ccgagcgttt tgagccgtcc gagacacgcc ggaacgcaca gcgccgccat
    25141 caccgccatg aaaagccacg ggcggctccc cggatagacc gctgtcacga accggtctgc
    25201 cccggcgcgc agcacgaacc cggcataaaa taccgtccac gccgtataga gaagcaccgc
    25261 cgccgtgccg ccgaagcgcc cgaacacgcg cagcagcacg ccgccgagcc cctcgccggg
    25321 gacgcgcttt cgcgtcagcc gcccgagcag gagccccgcg agcggcagcg gaacgagcgc
    25381 cagaagcgcc gagagccaga ccgcgctccc ggcgctctgc accgccatgc ccggcgcctg
    25441 ccggatgagc ggcgagagcg ccgaaacgaa aagcagtatg gaaagctggc ggcgggatat
    25501 gccgtcggtg cgctcatcca tgctctctct cctccttccc gtgggcgccg ccgtaaatat
    25561 cgtaggagcg ctcgagcacg ctctccgtct gcacgagagt gcgcagcgag gcgaggacgc
    25621 cctcgtcctt cacctccggc agcacacgcc acagctccag aaaatcggcg tccagcgcct
    25681 tggacgcctc cgccgccgcc tcgatctgcg cggcgacggc gcgggagagc tcgtcctcca
    25741 gccgggcgag catttccgcg tccgcggtgt ccgcgccgga ggattccgtc accccggcac
    25801 ggacgcgcac cgcgatccgg acggcggtct cgccgccgtt tcggacgggg gagagggaag
    25861 cctttgccgt gtgcagctgc accgtcaccg tgccgccgct cccgtcgggg aggacgtagc
    25921 ttgcctgcgg cgcaaagccg agaagaagat ccgcgccgag cgcggcgtcg gtatcgagaa
    25981 agccgcagag cgtttcgccg cgaaaaacgg cgtacccggc ctcgagcgcg agcgttgcgc
    26041 cgtcctccgc ttcgggaacg ctcctctccg gcgcggtagg ggcgatcgcg caggcaagcg
    26101 ccgcgccgct gcggttgagc cgcgaggcga tctcgagcac cgtgaagcag tgcgcgctgc
    26161 cctgccgctt ggtgtcggcc tcaagcgagg agagcgccgc ggtgatctcg ttctcgccgc
    26221 cgtttgcggc gacgagagta taggcgctct cgcccttcac gacaaacagc ggcacgctca
    26281 gctgcgtctg cgtgccgcgc tcaaagtaat cgagaatggg cgtaatgccg ccgcgcgcgc
    26341 agtcctcgcc cacggcggcg taccggatgt gcgcaaaaaa cagctcttcc cgcggcgaaa
    26401 agtcctgcag acgctgcacg gcggtctgga tgctcgcgcc gcgcgcggcg agctgcgccg
    26461 ataccgcgtc ctcgctcgcc tcgcccgagg agacggacac cgtcatgtcc ccgatgccgc
    26521 cgtcaaaccc gatcgtctgg atgagctgaa gatcctcgac cagccgcggg tgcagcagag
    26581 acgcgcagcc tgaaagcagc gcgagcgcgg cgagcgccgc gctgagccgg aaaaagcgct
    26641 tcatttccga tcctcctttc gcagcggcgg ccggagaaag gcgcgccgca gagagccggg
    26701 gcgcgaatcc gtgagcggtg aaagatagct cacgccgaga ttttccgtgt cgcacagccg
    26761 ccacaaaagc agcataagct ccgccatcac gccgtaaagc ccgagcacca ccgcgccgag
    26821 cacgagcgcg acgcgccaga gccggatcgc cgcgccgagc tcctggctcg gctgggcgta
    26881 tcccgcgatg cccgccatgg cgactactat aattacaatg gggcttaaaa ccttcgcctc
    26941 taccgcgctc tgcccgacga tcagcgcccc gatgaccgag accgtctgcc cgaccgggtc
    27001 ggggaggcgg atgccggcct cctgcagcag ctcaaacgag agcagcatcc cgagcgtttc
    27061 cgccgccgtg gagaagggca cggactgctt ggaatcgatg acgttctgca ataatttata
    27121 tggtatcatc tcctggtggt acatcgcgat ggcgacgtac agcgccggaa gcgcgagcga
    27181 gaggaagagc gccagatacc gcagcagcac gagcgccgtg gcgacagcgg cgtggcgcgc
    27241 ccggtcctcc tgcacgcgca gcatgtccgg cagcgtggcc gggagaagaa agccgatggg
    27301 cagtccgtcg cagagcacgc cgatcttccc gcgcgcgaga ccgagcacga accggtcggg
    27361 ccgctcggtg tggatcacct gcggaaagat gccgcggggc gttccggcaa ggtattgttc
    27421 cacgtctccg gcggcgagca ccgccgggat atcggggagg gaaaggcgcg ccttgatgcg
    27481 ctcgacctgc tcgggcgggg cttcgccttc tatatataat atatccaccc tcgttttcgt
    27541 ctccgagccg agcatcgtct cccagagctt gagtgacggc ttcccgacgc ggcggcggag
    27601 gagcgcggtg ttcacgcgca cggattcgac gaaggcgtcc ttcgcgccga gcacgctctt
    27661 ttccaccgtc ggcgcgttca cgccgcgctt gtcgccgctc ttgacctcaa aggtgagcgc
    27721 cgtgctctcg ccgtcgaaaa cgagcgcggc gtaccccgcg gcgatatccg agaccgcttc
    27781 ctccggggag gaacgctgcc ggacggtgca ggcccacacg ccgcccccga gcgcgcgcgt
    27841 gagaagatcg tgttcggagc gggcgcgaag cagtgagagc ggacggacga tctcgctgct
    27901 gatctgcgcg gcgctgacgc atccgtccag ataaaaaagc gtgagcgaaa cgccgcccgc
    27961 cgagagcgcg cggctttcaa aatcggcgca gccggaaaaa gctttttcga tggatttcga
    28021 gaaatcccgc cgcgccggtg cgggatcgga agagagaaaa tcaaacatat gggaagtatg
    28081 cccagcggaa agcactatat gtagcggccg gaaaaaaaga tgaaaattct tcaaaaaaac
    28141 tttaaaaaag tgttgacaaa aggagtaagg gatgttatat tagctaagct gtcgccgaga
    28201 gacagggcga aagaaccgaa aaagtct
//
LOCUS       DAWAAE010000008        27800 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_746_length_27800_cov_3.128059, whole genome shotgun sequence.
ACCESSION   DAWAAE010000008 DAWAAE010000000
VERSION     DAWAAE010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 27800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 27800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..27800
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_27800_cov_3.128059"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    489..498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3055..3064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6153..6162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13749..13758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14855..14912
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23675..23684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5249 a         8087 c         8169 g         6187 t
ORIGIN      
        1 gcgggagagg tcgagcgcaa gatagatgta cagccgctcg ctcgaaaagc cgggggacga
       61 atgatacgcg ccgagcggca cgatgcgtcc ggcggtgaag ccggtctcct cgccaagctc
      121 gcgcaccgcg cacacgagcg gatcctcccc gcgctcgagc ttgccggcgg gcgcttcgag
      181 caggtgctcg ccgatgggat agcggtactg acgcacgcag taggcattgc cctcgccgtc
      241 caccgggaga atgcacgccc cgcccggatg ctccacgatc tcgcggaacg cctccgaacc
      301 gtccggcagg ctcacgcggt cgagaccgac gttgatgatg atcccctcat aggtattgag
      361 acggcgcagc tttttctccg tatagtccat ggatgatctc cttttttctt tcgtatcagg
      421 gtattctacc acggctctcc cggcttttcc agcttttttc actgtttatc ggcggcggag
      481 gggcgtatnn nnnnnnnngg ggcgtataat ggggaaaaac cgcgatttgg ggaggctgtt
      541 atgcagaaca aagcagaaaa cgaacgggcg ctcgcgctgc gcgtgccggg gcacccgggc
      601 ctttcggacg ccaaaaagct cgttttcgcc gcgctcggcg gaagaaactg gggcgtgctg
      661 gagatcgaga tcatcgctgg gcgggaggag agcctgctgc tcgcgcgccc ggcgagcggc
      721 gtttacctgt cgcgcgaggt gctctcccgg ctcgctgcgc gcgggcgctg aaattctctc
      781 tttttcgctc ttgacaggta tgatataata cttttactgc caaggcggag gaggtggaag
      841 gccatgccga aaaaagaagg gatcaaggag atctcttcca accgcaaggc gttccacgac
      901 tactttgtgc tggagcgctt tgaagcaggc atcgagctgt tcggaacgga ggtaaaatcc
      961 atccgtgccg ggcaggtgaa tctgaaggac agctactgca cgatccggga aggcgagctt
     1021 ttcgtgcgcg ggatgcatgt gagcccttac gaaaagggca acatcttcaa ccgcgacccg
     1081 gtgcgcatcc ggcgactgct gatgcacaag cgggagatcc gcaagctcgg cgagcgcgtg
     1141 gcgcaggacg gcgtggcgct cgtgccgctc tcgctgtact tcaaggacag ccgcgtcaag
     1201 gtggagctcg gtctctgcaa gggcaaaaag ctgtacgaca agcgcgattc ggacgccgag
     1261 cgcaatgcga agcgggacat ggaccgcgcc atgaaagaaa ggaattccaa agaatgaatc
     1321 ctgttgtgac catcgaaatg gaaaacggcg gcgttatcaa agccgagctt taccccgaga
     1381 tcgcgccgga gagcgttcgc aacttcatct ccctcatcaa caagggcttt tacgacgggc
     1441 tgatcttcca ccgcgtcatc cccggattta tgatccaggg cggcgacccg gagggcaccg
     1501 gcatgggcgg ccccggctac cacatttacg gcgagttccg cggcaacggc ttccagaacg
     1561 atctcaagca caagcgcggc gttttgagca tggcccgttc catgtacccc aacagcgccg
     1621 gcagccagtt tttcatcatg catgaggacg ccccgcatct cgacggacag tacgccgcgt
     1681 tcggcgccgt caccgagggc atcgaggtcg ttgaccagat cgccggcacg cgcaccgacc
     1741 gcaacgaccg tcccatggag ccgcaggtca tgaaaaaggt caccgtggag accttcggcg
     1801 aggaatatcc cgagccgaag aaaagataaa aagatacagg aaaagatttt ctgaatcttc
     1861 cttgtcccaa agaataagga gcaatttatt tgaatttatt ccttattcca aaaagaaggg
     1921 gtcctcaagg gggaacatgg ttcccccttg aagttgcctc gccgaaggcg actctgccgt
     1981 ttccgcagaa acggcggcaa ctatgggggc gtaccggttt cgacggggat gcggagattg
     2041 gaatagcggg cggatgcgcc taccatcctt aaaatgggca attataaatt aaagaacaac
     2101 gataacgttg ctctggccgc ggcttaagcc gtgacccgtc ccctccggga gtgcctcggc
     2161 ccggaagcgg ggcgtcgatg agaggcggac gtgagcgcgc aaagctttga gcgcgccatg
     2221 tataatgaag ctacccgact tgccgtcttg acgtttggcc ggtaacgagg gaaacaggga
     2281 tgcagaatcc caaaacaagc gtctgcgccc ggagaagttc cctttgaagc gttttcggac
     2341 aggagttcaa ctctcctcgc ctccaccacg tcaaaacaac accatgaggt catccgccgc
     2401 cgcaagcggc gtccgatcga ccgatggtgt tgtttttcct ttccaaattg aacccgctgc
     2461 gccgggtttg atctccggca ttttctttgc atacaaatca tgggagcggg cggatcaact
     2521 atgagaaaga tactttgtct gctgaaaaaa gagcttatgc tcacaatatc ggttttgatg
     2581 gcatgtctat cgctctttct ttcaaaaccc ggcgttgaaa cgctcaggag catcgactgg
     2641 cacactctgg cgatcctttt catgatgctg acggtgctgg agggtttcaa aaaagaaaac
     2701 atttttcagc cggttttgag gttttccgaa aagatcactt ccatggtcgg gatctcgttc
     2761 tttttaattt tcggtgtgtt cttttcctcc atgttcgtga cgaacgatgt atcgctgatc
     2821 gttttcgtgc cgctgacgat ccttctcttc cgcacggcaa aaaaagaacc gtatatcctg
     2881 ccggtcattt caatggaaaa tatagcggcg atccgcggtt ccttgctgac cccttttggc
     2941 agtccgcaga acctgtttct gtttgagcag tccggcattt ccgccggaaa gtttatgctt
     3001 catatgctgc cgctgtggct ctcctccgca attcttctcg gcgggttcat cttcnnnnnn
     3061 nnnntttgct cggaagctcc ggagaagcat ggaagccgga gcggaaaaag cacagaatta
     3121 tctacgttgg gctgtttgcg ctgactgttg caacggtcgt ctcccgaaca ccgtattggc
     3181 tggctgccgc cgggatcgtt ctcgccgtta ttctgctttt tgaccgcgcc gtactgagga
     3241 aaacggacta tgttctgctg gtcaccttct tctgtttctt tgtcttttca tcgcagatca
     3301 ctgcgaacaa gacgatctcg cactttctta ccaacgccgt tgccagccac gagtatttct
     3361 ggggaatcgg tttgagccag atcatctcca acgtaccggc ttccattgtc ctttaccctt
     3421 ttaccgataa tctggccgct cttctgtacg gtgtggatac ggcaggtctg tgctccgtca
     3481 tcggttccct cgcctcggtc atcaattaca ggctttatgt gcgctcttac ccggggcaag
     3541 gcgggaggtt tatgaaagtt tttacgctta tcagctgggc atttttcctg atcgtggtca
     3601 ttcccggcta ctttctttca aagtactggc tgatttagta taccatctgc ccacctccgt
     3661 gcggtggatc tcccctgtgc agggtctccg ttttcctgca cgggggtttt tgtttgcagg
     3721 agagtctgca agccgttcat aaattgttta cgctccgccg gttgactttc ggggtgcggc
     3781 agattataat tgcaaatatt ctaatattag agcttctatt cttggaggtt tttatgccag
     3841 agcagaattt caccgcttcg cagatgttcc gcaacgcgaa ccggtttatt gaagcatacc
     3901 atgccgcatt gcagccggtc tgccgggata ccggcctgcc gccgatggcg gtcgatattc
     3961 tgatgttctt cgccaacaat cccgaaagcg gcacggcgaa ggacgtctgc cagtgccgcg
     4021 gcttcaaatc cggcattgtg tccgtccacg tggaccggct cgtgaacgag gggcttctct
     4081 cgcgccggga ggtccccggc gaccgccgga aaacgcggct cgtgtgcacg gacgcggcgg
     4141 cggagatcgt agagcgcgga cgcgcgctgc aaaaggcctt tgcgcaaaaa ctggtggacg
     4201 ggctgtcgga ggcggatgtt gccgtattcc acagctgtct gtcagcaata ggcgacaaca
     4261 tagaggacat ccgcaaaaac ggactttttc cggagaacag gagataacga tgctgaaact
     4321 aataaagaat atgcgccgga aggaaaagga gatgacgctg ctctgtctgg tgttcgtcgt
     4381 ggtgcagatc tattttgatc ttcgtctgcc ggactatatg acggagctca cgatgctgat
     4441 caaaacgtcc ggcacaacgg cggacattgc cgccgtcggt ctcaaaatgc tcggccgcac
     4501 ggcggcaagc gcggtgcttg cgatcgcgtg cggctttctc gccgcaaaaa ccgcgtcggg
     4561 attcagcttt gcggtgcgcg aaaagctctt ccgccacgtg atggacatcg gcagcgagga
     4621 aatgcaggac ttttccattc cgagccttat cacccgcacg acgaacgaca tcacccaaat
     4681 tcagatgatc atcgccatgg gcctccagat gatgatcaag gcccccatca tggcggtgtg
     4741 ggcaatcatc aagatcctcg gcaagagctg ggagctctcc gccgtgaccg cggcgttcgt
     4801 cgtcgtgctg ttcacaacgg tactgctcat tatgggctcg tgcattcccc gtttccgtct
     4861 tgtgcagaaa atgacggata aaatcaaccg cgtggcgcgc gaaaatctga ccggcatcaa
     4921 cgtggtacac gccttcaacg cggaggagtt ccagaacgaa aagtttgacg gtcccagccg
     4981 cggcatgatg gatctccaga tgaaaaacca gaagctcttt gcgctggtgc agccgtcgat
     5041 gtcgctcgga atgaacggtc tggcgcttgc gatctactgg gtcggcgccg cgctcgtgaa
     5101 cagcattgcc gtaacggaca tggccgcgcg ccttaccatg ttcagcgaag tgctcgtatt
     5161 cagcacgtat gcgacgtata tcgtcatgtc gttcatgatg ctcgccatga tcttcatgct
     5221 gctgccgcag gcgcaggtgt ccgcggagcg catcaacgag gtgctcgacc gcaaagcctc
     5281 catccgcgag ggcagcgtcc aaaccggcgc ggagaccgga acggtggagt tccgaaacgt
     5341 ttcgttccgc tacccccacg cctccggaga cgcgctgacg aacattgatt tcaaggtaaa
     5401 ccgcggcgaa acgctcgcga tcatcggcgc gaccggcagc ggcaagacaa cgctcgtcca
     5461 gctcatcccc cgcttttacg acgcgacgga gggcgcggtg ctcatcgacg gcgtgaatgt
     5521 gaaggactat tccttcgatg cgctctacga taagctcggc tatgtcacgc agaaggccgt
     5581 tctcttctcg ggaacggtgc gcgacaacgt actcttcggc gagagcgctg ccggaagcga
     5641 cagcgaaaac cttgagaacg ccattgcgct ttcgcaggca aaggagtttg tggataagct
     5701 gccggagggc acgaactacg agatcgagca gctcggccgc aacgtgtccg gcgggcagaa
     5761 gcagcgcctt tccatcgccc gcgcgctggc gcgaaagccg gaaattctgg tctttgacga
     5821 ttcgttctcg gcgctcgatt accggacgga cgcgaagctg cgcgagggtc tgaaccggga
     5881 gctgcatgat gtgacaaaga tcatcgtcgc ccagcgcatc agcacgatcc gccacgccga
     5941 aaagatcctc gttctcgacc gcggcgagat cgtcggcatg ggaacgcacg acgagcttat
     6001 gcaattctgc aaagtgtacc gcgagatcgc aacatcgcag ctgagcgcca cggagctcgc
     6061 gtaaggaggg gcggaaatga aacgaaccaa taaagcaaac ggcatccgct ccgcggtgcc
     6121 gcgccttacg gactatatgc gctcaagcat ggnnnnnnnn nntcatcatc ctggcgctga
     6181 tcctcgccgc gctcagcgcg gtgatgacga tcctcgggcc gaaccagatc ggccggatcg
     6241 ccacgctgat gtcggacggc ctcaccggaa acatcgatct cgcggcgatc gccaaagtcg
     6301 gcattttcct tgctgtcatc tatgtactca gtgcagtttt cagctttatc cagcactaca
     6361 cgatgtctgt cgtcacgctg aaaatgtcct accgcatgcg cggcgagctt tccgccaaga
     6421 tcaaccgcgt gccgcagaag tatttcggca cgacctcgca gggcgacatt ctcagccgca
     6481 tcacaaacga cgtttccacc ctgcagcagg gactcacgaa cagcctgccc acgatcatca
     6541 gcgcggccac gcagtttgtc ggctgtctcc ttatgatgtt cgtcaccgag tggcggctcg
     6601 cgctcgtttc catcggcgtg acgatcctcg gcatgctgct gacggtgctg atcctgtcca
     6661 gatcgcagcg gtatttcgcc gcgcggcaga aaagtctcgg cgcgctgaac gggtatgtgg
     6721 aggaaatgta ctccgggcat gaagtcgtgc ggctcagccg cgccgggcgg aagatcggcg
     6781 cgcagttcga tacgctcaac gccgccgttt acgacgcaaa ctggagatcg cagtttctct
     6841 ccggcatgat gcagccgctt atgaacattg tcggcaacat cgcctatgtc gcggtgtgcg
     6901 ttctcggctc gatcctcgcg atgaacggca cgatcgagtt cggcgtgatc gtctcgttca
     6961 ttctgtatgt ccggcttttc acctcgccgc tgacgcagat ggcgcagggc atgacgaaca
     7021 tgcagaccgc ctccgcctcc gcgcaccgca tcttcgattt tctcgaaagc gaggagatgc
     7081 ccgacgagag cgaaaagacc gcgaagctca ccgatgtgcg cggcagggtc tgctttgacc
     7141 acgtccggtt cagctatccg agtgacccgg acaagatcat catccgggac ttttccgccg
     7201 aggttctccc cggacagaag gtcgccatcg tcggcccgac cggcgcgggc aagaccacaa
     7261 tggtcaatct tctcatgcgg ttctttgaga tcaacagcgg cagcataacg atcgacggcg
     7321 tgcccatctc cgatatgcgc cgggaggatg tacacggtct cttcagcatg gttttgcagg
     7381 acacctggct ctttgaggga acggtgcggg agaatcttgt gtacaacatg gagggcatca
     7441 ccgacgaaca gctcgaagcg gtctgccgcg cgtgcggact cgacaagctc atccattcgc
     7501 tgccgcaggg gtttgatacg gtgctctccg agagcgccgg catttccgcc gggcagaagc
     7561 agcttctcac catcgcccgc gctatgctgc aaaatgcgcc catgctgatc ctcgatgagg
     7621 cgacctcctc cgtcgatacc cgcacggaag ttttgatcca gcgggccatg gacgagctca
     7681 cgaaggggcg taccagcttt gtgatcgcgc accgcctgtc cacgatcaaa aacgcggaca
     7741 agatcctcgt cctgcgcgac ggcgacgtgg tcgaaagcgg cacgcacgaa gagctcatgc
     7801 agcgcggcgg cttctacgcc gagctctata acagccagtt tgacgaggca tcctgacttc
     7861 tttctccgtt tgaagcggaa agacgcaggt tcccgatctg taaacagcac cccatttgtt
     7921 agacaagtac gataaaatac ttgtactaag gaatggggtg ctgttccata cgacacttgc
     7981 gggattttct cttatttcgc gagagcggcg acctgctcgg ccatggtggg aacatcgcat
     8041 ttcccggtgc ggatggcctc cagaatgacc tccggcgagg ccgggacttt gttgatgcgc
     8101 acgccgacag cgcgggaaac ggcgttcaaa atcgccgggg tgatcggcac ggtggcgacc
     8161 tcgctcacgc ccttcgcgcc gtaaggcccg ataggttccg ggtcttcgat gaggatgagc
     8221 ttgtagcgcg gcgtgtcctc cgctttcgga agccccatct gcccgtagag ctttttgacc
     8281 gggtagccgt tttttgtcgg gtggccctcg gtgagcgcat atccctgtcc catcgacacg
     8341 ctgccctgca tctggccttc gataatgcgc ggattgatcg cgcggccgac atcgtgcgcg
     8401 gcgatgacct tgaggagctt taccttgccc gtggcggtat ccacctcgac gatcgccgcc
     8461 tgcgttgcgt aggcgtaggc cgggaagttg cggtacttta cacccttctc ctccgcgccc
     8521 ttgaaatttc cgatggcgaa gcactccggc gcgcggaagt agtattccgc gtaccggctc
     8581 tcgcccggct gcggcggatt ctcttccagc tccttctgca gaaggcggca cgcttcgtac
     8641 actgcgcgcc cgtcgcagag cgtctggcgc tcggacacgc tctcgccggt cgggatcgtg
     8701 atgtccgtat cgccgatgac gatatcgata tccttcatat ccatgcgcag cgtttccgcc
     8761 gcgatctgca ccatggcggt gcggaagccc tgtcccatgt ccgtgccgga gacgatcata
     8821 tggagcttgc cgtcgccgtc gagcgtcagc ctgcacgcgc catcgtcaat gaaaatgccc
     8881 ttgccgatac cggagttttt gaatccggac gccacgcccc agccaaggac cttcgttccg
     8941 ttcgggtact gcccctcgta ttccctgagc gcttccctga ccgccttttc gcacaggtcg
     9001 atcgtatcgt gcatacccat gctgctctcg agcagttctc cgccgacgga gcagctgccc
     9061 gggtacacgg cgttgcgccg ccgcagctca aaggggtcga tgccgagctt ttcggccatt
     9121 tcatcgagcg ccgtttcgat ggagatcgcg gcctgattga tgccgtagcc gcggaacgcg
     9181 ccgccctgca gattgttatc gcggatacag cgggcgtcca cgcggacgtt cgggatgata
     9241 tacggcccgc cggacatcag acagccggtc atgagcgtac cgtagctctc ggcctcgtag
     9301 gggccgccgt cggagaacat gaagctgtcg agataccgga acctgccgtc cttcgaaaag
     9361 ccgatctcat aatccgtgtg gtacccgtgc cggcgggttg tgacgatcat gtcctcctgg
     9421 cgtgtgagtg ttaccttgac aggcttatcg cagcagagcg ccgcgaccgc cgcgggcgct
     9481 tcaaggaacg agtcgcattt cttgccgaag ccgccgccga gcggcgtcgc cacgacgcgg
     9541 atgttctcct cgtccatggc gagattttta gcaagcatcc ggcgcacctc aaagggcgcc
     9601 tgcgtgcagg cgtacaccgt gactctgccg tcctttgcgt acccgatggc gctcaccggc
     9661 tccatgcaga cgtgatcctg cggctctata tccttcgata cgcgcacctt tacgatctcc
     9721 gcctcagcct tgcatttttc cacatcgccg gtctcgcggc cggtacggac gatgtagctg
     9781 tccgccttca cgccctccgc catcgtatag atgccaggca gctccgtata gtcgatcttc
     9841 acaagtttcg ccgcggcgcg ggcatgctcc ggcgtgtcgg cgacgaccat ggcgacataa
     9901 tcgccgagga agcgtacctc atccgagcag aacaccggct ggtcgggcgt ccatgtgccg
     9961 atcttgttga gccccggcac gtccttatgc gtgacgatgc gcgcaacgcc gggcgcggac
    10021 tccgccgcgg aataatccac cgcgttgatg cgcgcgtgcg gcacgggcgc gaaaacgaac
    10081 gcgccgtgga gcatcgtatc ttcgccgaat tcatcggcct cgtagtcgtc gcagtatttg
    10141 agcgttccgg cggttttggc aaggccgtcc acgtcccaga cgctctgccc gacgaagcgc
    10201 ccctcgatct ccgggacgat ctgtccctta cccgtgacga tctcggtcgg gtcgttcacc
    10261 tgcacggcag tgagcggcac atcctcgccg cgcagacgcg ccgcggcgag cttcacgccc
    10321 tcgatgatct tcacatagcc ggtacagcgg cagtagttgt gcttcaggcc gtcgtatata
    10381 tcctcttccg tcgggttcac ggttttgcac agcagcgcct ttgctgccat gatcatgccc
    10441 ggcgtgcaga agccgcactg caccgcgccc gcgtcgagga acgagcgctg gatgacctgc
    10501 agcatcgtgt cgttcggaag attttcgatc gtctccacgc tctttccggc gagcgttttg
    10561 agaaccgtta cgcatgagcg ggcaaggata ccgtcaatga gcacactgca cgcgccgcag
    10621 tgaccggtgg agcagccttc cttgctcccc ttcatacact cgacattgcg cagatacttc
    10681 aaaagcgtca tgcgcccctc gtatgccgcc gtaacctctc tgccgttcag gataaatgtt
    10741 acactttcca cgtcgcggta atcccccttt tattttgttt gcgcaaagtc ctatccgtta
    10801 ttcttgaatg ccttttgtct ttttcgcgtt gatccgcgca gtattcacca tgccacagaa
    10861 aatggatacg ccagcgcttg gcaggcgcag ggatctcttt tcttatgttg ccgacgcggc
    10921 tgcatccgca taccggatcg tttccatctt tctgcggaag cgccgctgca acgaccgttg
    10981 caaaactctt ccgagtgaag tatactattt atattcttga ttcccgagag gaaggttttt
    11041 ctgttggacc gctgtgaact gcttcttgac ctgctgcttg aatacaccaa ccttgccgaa
    11101 ataaacgacg atatccacaa tgtgccggac ctcacgggct acgataattc ggagctgaac
    11161 tgtctggact gcattggccg gatgaaagat cccaacgtca caagcatcgc cgaagctatg
    11221 aatatgacca agggtgctat cagcaagatc atccgaaagc tctctgacaa ggggacgtta
    11281 gtggattacc agctggacgg gaaccggcag aaggtgtatt acaccctttc ggacaagggg
    11341 aaaacactgt ttgccgctca cgaagcccgt cataaccaat ggcgcaaccg ggaggccgcc
    11401 tttttccgta cgctgtcgga gaccgatcgc gaaggcgcca tccggttttt gaccgcctat
    11461 attcacgagc tcaacaacag aacgtctcct tctccgtctg actgagtcgt atccgtcacc
    11521 ggcagaccgt ttctcttgta cttgtttcct tggatataag ttgatactat ccgattacaa
    11581 ggcacatgtc aacatatttt catttaagaa caattcagaa gctgctcaaa accagtgaaa
    11641 actgctgttg ggcagctttt gagatattgt ttgcgatttt acttgacata caaagaatgg
    11701 tctattttac ttgtttcccg tgtctgccgg agctggggcg gcttttttgc tgagtccgcc
    11761 tctctcttat tctcaagcgc catccttttc tcttctttgc cgcggcgcag gccgccccgc
    11821 cccctttccg aagggggcgg ttttttcgga taaaagagag acggaagcag cgtcagcctc
    11881 cgtctctcct ttttattttt tgtcaatccg tcgttccggc ccagcgatca acgaccggag
    11941 caagttttgt gatcgtcggc cccatgatcg gcccggtggc aaacatgccg agaaccgtgc
    12001 cgagaccgat cacgccgccg agcgcccatc cggccaggga gaaaacgaca tcacatccga
    12061 tgcgctccca tttcagaggg atcttcagtt tgtcggagag cgactggtta aatccgtccg
    12121 tcggcccgca gccgagccga gccgactgat aaattcccag accgagtccc aggccggcaa
    12181 caccgatcag caggaatacg gcgcgaagca caagcgtcgg ttccgtaccg aggattggat
    12241 ggaaaagtcc gataaacaga ttgcaaaacg taccgcagag gaaggtatag attacggtgc
    12301 cgagatggat gtacttgcgt cccatgataa ggatcacaac aaagaaagcg atattgtatg
    12361 cattcatcgc cgcgccgaag ctcttgagat gaaatacgga ctggatgccc tgcacaaaca
    12421 ccgtgaccgg gtcactgccg agcgtcgcaa cgacgtaagc cgcgcagccg acgccggcaa
    12481 cggctgtgcc gataaggatc acaaggcagc gcacagcaat gttccggaat gtccaggctc
    12541 tgtgtgacat attcgttccc tccctttccg tccagcgttt agggctgctg gctcttccac
    12601 tcaccgacaa gatcagcgga atcgaccaca aggccgagat gcagcgagac catgcgcagc
    12661 gctgcttcct caagatgcgc cgagccgcgg gtcagatcac gcgggacgac tacgcggaag
    12721 tcccggttgg cggcgtcaaa tgccgtattg agcacgcagc agtccgtcat gcagccggtc
    12781 aggatcacgc ggcggatgcc gagattgcgc agcagcatct ccatatccgt cggatagaac
    12841 gcgctcaagc gtttctttcc ggtgaccatc agatcgccgt cttccacgtt aatgctcatc
    12901 ttgttccaac gggtaccctc aatggcgtgc tcgtctatgt tggggatctt gcctacaaac
    12961 atcgggaata ccatgcgcca cgcagaagga tagagcttct cgtccacgcc gccctttcgc
    13021 agtgtagagc gcacatggat caccgggata ccgagagagc gcgcctccgc ggtaaaggca
    13081 ttgagcggct caatgatctc gcggccacgc aggcacgggc aggggcagtc ggggtcttcg
    13141 gagaggtgcc cgtcgtgcat atcgatggtg atgacggctg tgtcgtgacg gtcaagaaat
    13201 actttcgcga gatcttcggg aacttccttg ggaacattgt caaaatacgc tttcatggtc
    13261 attattcctc tcttttcttt ttggggatgg ttttccttcc ccgtgtctgt atcgtagcac
    13321 agaccggccg gatgcggttt atgcgccgcg cacaataatc ttttttaaat attgtgccac
    13381 tcgccaatat attttttata tgtcggacag cttgttcaat atataacaaa aaccggagga
    13441 ccgacttttt tcaaaagcga ttctccggtt ctttatctct ggcagtagcg gtatatttta
    13501 agataattga aaagcatatt tcggtgctcg gcatcctcgg tatccatgtt cagcagttca
    13561 cggatctttg tgagctgata ggttacggtg ttgcggtgca tatacagccg ttccgccgtt
    13621 tccgtagcgc tgccatttgt gagaaggtag gtttccaatg tctgaaggag attcttccgg
    13681 ttttccagat catagtcgag cagcggcttg agccgggaga cagcaaattc ccgcactgcc
    13741 tcctgatcnn nnnnnnnnat cccaagctct tcaaaaagcg tcacaggctc ccggctgttc
    13801 atgcccgcag cgcgtccggc ctcgtctgca ctgcgccgaa gcagcagcgg ctcttcgtat
    13861 atctcgccaa caccgcagac aggtgccgcc agaatccatt gtttccgtat gctccggcaa
    13921 acagaaagca gcgttttttt cagtctcgga agctcgcttc gctccagccg aacgagaaaa
    13981 atgatctcct gctgccggtt cagatagagc gttcccggga aggcgcactt aacgtcctgt
    14041 atcatacagt tgaccgcctg gcggcgaagc gtatcgctgc gaaagctgta ctccgcctcc
    14101 gttaccggaa agcgcgctac gaccgccgtg tgcggcccgg caacgtgctg cttcttaatg
    14161 aagcttcgca gcgtttccga cggtacgccg ccgttatgaa acagcagctc ctccaaaagc
    14221 gaatcgaacg cctcctgccg gtagcgctcc agcagcaaaa gctgggaaat atctgccacg
    14281 acctcggcaa tgtgcgtatc ccatggtgcg gcaaaaatcg gaaaagcgct tttttcggcg
    14341 aaaaccgccg tctgtgtcgg gatggcgggg atgtgttccg gggagagcag cacgataaga
    14401 cacgccgctt ttttctcaac cgcctgctcg acgatgctgt tgagaaattc gtcggtgcat
    14461 tcaagaccga tgcccgtcat tataatgacg tcaccgccga tcagccattc gcgtatgctc
    14521 ggcgtctgcg caatgtttgg ccaggacacc gggcgataca gcccgccgtg ccccgcagcc
    14581 agcaccatcg gtcgaaagga atcgagtgag agcaagtctt taacgcaagc catcgttctc
    14641 cccctatttt cttgtttcca ggttaaccaa ttttagtata gcacaggaag agaaaaactg
    14701 tttattctat tttaagaaca attcttacag gttgaatatg ccgtagagct gcttgtactg
    14761 cagcacacgg taaacgctct cgttaaggcg cgcttcggat atcgtgccgt tttccacggc
    14821 ggcagcgacg gcgtcaaacg cctcggacag taccnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcataagg atgatatcaa cgcccgcctc
    14941 gatcgcggcg atcgccgcgt cgccggacga ataggcgtcg gtcacaccgt gcatcgccat
    15001 ggagtcagtg atcaccagcc ccgtaaagcc cagctcgccg cggaggtatc cggtgatcac
    15061 cgttccggaa ggctcgcggg cgtgagatcg ccggtgacgt tcggcgcggc gatatgcccg
    15121 accatgacgg cgcagccggt caggtcgttc atgctgtagg ggatccactc acacgcggca
    15181 agctcgtcat gcgtcttata tgtgacggca aggccgttgt gactgtcctc ggcggtgtcg
    15241 ccatggccgg ggaaatgctt atacaccggc agaacgccct gcgaggcgag cccccgcgcg
    15301 cacgcgcccg ccatggccgc ggcggtattc gcgtccgcag agaatgcgcg gctgccgatg
    15361 acggtattgg ccggattcgt atatacgtcc gccacaggcg caaagtccac cgtgaagccg
    15421 tagtcggcga gatagccgcc aatcgtgcga cccatgttct cggcatccgc cggattcccc
    15481 gacgcgccca cggcggcggc gctctcgtat ttcggcagac cgaaggcggc gttattggca
    15541 agccgcgcaa caagcccggc ctcctcgtcc acgccgagga acagcggcac cgttgtcgcg
    15601 tcgcgcagct gttcggtgaa cgcgcggagc gtttcggggt cagagatatt tttgccgaag
    15661 atcgcaacgc cgccgaccgg gtacgccgcg agcaccgcgc gcatatcgtc catgagcgtt
    15721 ttcacgccct cggtttccga ggagttgatc tgtcccggcg tctgcgttgg atcgagcgcg
    15781 tccggacgga tgaaaaagag ctggccgacc ttttcccgca gcgtcaaggt ctgcatatag
    15841 cgctcgatgg cgtcgccctc tgccggggct tccgtctctg tcggggtctg tgccgccggt
    15901 gtttccggcg taccctccgg ttcgggcgtg gcagtaggtt ggggcgtcgc cgtcggctgc
    15961 ggtgttgccg tgatttgcgg cgcggcggta tcctccgcct ccggataaca gggttccgtg
    16021 cccggcgcac cgcaggcggt cagcagagta catattataa gcagcattgc aataggaata
    16081 cacaattttt tcatctttac gttcccttcg cgcttttctt atggtattag acgcgtttct
    16141 cggcggtttt gttcggcatt ttgcaaaaat ggcgcagcct taaaaaggct gcgccgtttt
    16201 ttgcgtttta aacgggatta caccagctcg atgaccgcca tttccgcggc gtcgcccttg
    16261 cgggcgccga tgcgggtgat gcgggtgtag ccgccggtgc gctccttata cttgggagcg
    16321 atctcgtcga aggtgcgctt ggcgatgtcc tcgcgggtga tgaaggagca cgcctggcgg
    16381 tagttgctca gaccgccttc cttgccgagg gtgatcatct tctcagcgag aggagcgatc
    16441 tccttggcgc gggtgaccgt ggtctccata cgtcccttgt ccaggaagtc cgtgacctgc
    16501 tggcggagca ttgccatgcg ctgatcggta gtcttaccga gttttctgtt ggcaggcatt
    16561 gttatttcct cctatttact ggttgttgtc gtcgctggcc agggacaatc ccatcatggc
    16621 gagcttgtgc tcgacctctt cgagagattt gcggcccagg ttgcggacct tgatcatctc
    16681 gtcctgtgtt ttcgagatga ggtcctcgac ggtgttgatg tttgcgcgct tgaggcagtt
    16741 gaagctgcgg accgagaggt ccagctcctc gatcgtcatt tccagcacct tgtcctgcgg
    16801 ctgcggctcg cgctccttga ccgtgacggt tccgccggcg gcgtccgaaa ggtcggtgaa
    16861 aagcgtgaag tggtcgcaga ggatcttggc gcccagcgaa acggcgtcac gcgccgtgat
    16921 cgtcttgtcc gtccatactt cgatggtcag cttgtcgtag tccacctggt tgcccacacg
    16981 ggtgttctca acggagtagt tgaccttcag cacgggggaa tagatcgagt caacggggat
    17041 ggtgccgatg atgggctgtg ccggcttgtt gtgctcagcc gtgacgtagc cgcggccatg
    17101 cccaacggtc agctccatgg aaagcgagcc gtccgggccc agcgtggcga tgtgatgctc
    17161 gggattgagg atctcaacct ccgcatccgt cttgatgtcg ccggcggtga catcgccctc
    17221 gccggaggct tcgatataga cggtcttggg agtatcgcag tgaagacggg cgatgatgcc
    17281 cttcacgttg agaacgatct cggtcacatc ctcggtcacg ccggggatgg tggaaaactc
    17341 atgctggacg ccggcgatct tgatcttcgt gaccgccgtt ccgggcaggg aggagaggag
    17401 aacacgccgc agggagttgc ccaaggtcgt gccatagccg cgctccagcg gttcgacaat
    17461 gaatttgccg taggatgctt ccgccgaatt ttcgatgcac tcgatacgcg gcttttctat
    17521 ttcgatcatg tagtaccctc ctttagatgt cggcgccgtg ggatcggcgc cgcagatgtg
    17581 tcagcttacc aatcggacgg gggtattact tggagtacag ctcgacgatg aggtgctcct
    17641 cgatcggaag gtcgatatct tcgcgctcgg gcacgccgac gaccttggca accatgttgt
    17701 tggcatcgta ctccagccac ttcggaggct gacggaaggc attggcttcg acgctggact
    17761 tgatcttctc caggctgcgg ctgctgtcct tcagcgtgat gaccatgccc ggcttcacga
    17821 ggtagctggg gatgttgacc ttgcggccgt tcacggtgaa gtggccgtgc gtcacgagct
    17881 gacgcgcctc ggcgcggctc atggcaaagc ccagacggta gatcacgttg tccagacggc
    17941 tctccaggat ggcgagcagg ttctggccgg cctggccggg acggcgctcc gcgagctcat
    18001 agtagccgcg gaactgcttc tcgagcacgc cgtagtatct cttggctttc tgcttctcac
    18061 gcagctgcag gccgtactcg gagttcttgc tgcggccctg gccgtgctgg ccggggttgc
    18121 cgctgcggca ctccatggcg cacttgccgg tgtagcatct gtcgcccttc agaaagagct
    18181 tctggccctc gcggcggcac tgccggcaga cggcctcagt atatcttgcc attactctta
    18241 cctcctttga tcagacgcgg cgacgcttgg gcggacggca tccgttgtgg gggatggggg
    18301 tcacgtcctt gatcatcgtc acttcaagac cggcggtctg gagcgcgcgg atggccgctt
    18361 cgcgtcccga accggggccc ttgacaaaaa cttccacgga cttcaggccg tgctccatag
    18421 cagcgcggga agccgtttca gcggcggtct gcgcggcaaa cggggtgctc ttgcggctgc
    18481 cccggaagcc catgccgccg gcggaggccc aggaaatggc gttgccctgg gtgtcggtga
    18541 tagtcaccat ggtgttgttg aaggaagagc tgatatgcac ctggcccttc tcgatatttt
    18601 tccgctctct gcggcgggta cggactttct tgcccttctg tgcggtagcc atagtatcag
    18661 tttccctccc ttacttcttt ttgttcgcga tggtacgctt cggacccttg cgggtacggg
    18721 cgttggtttt gctgcgctgg ccgcgcacgg ggagaccctt gcggtggcgc aggccgcggt
    18781 aagaaccgat ctcggtcagg ttcttgatgt tcagagccac ctggcggcga aggtcgcctt
    18841 ccacgatgaa ctctttgtcg atgcactcac ggatacgcgc ttcttcctcg tcggaaaggt
    18901 cgcgcacgcg ggtgtcaggg ttgattccgg tctcttcgag aattctcttc gcgctcgttc
    18961 tgccgatacc gaaaacgtag gtcagaccga tctcgattct cttgtctctg ggcaggtcaa
    19021 cgccggcaat tcgtgccatg ttttgcaatc attcctttca atgattttat gccatgtctg
    19081 ggggtggctc gggcctatgg cataatgaag gccgacccca aaagggcagt aagaaattat
    19141 gccctttctt tcggattttc tatctgcgat tgatccgccg tagggcagat ctcagccctg
    19201 gcgctgcttg tgcttggggt tctcgcagat gcacataact ttacccttgc gcttgatgat
    19261 cttgcacttc tcgcacatgg gcttcacact gggacgcact ttcatggttt atcctccttc
    19321 gtgtcgcact ggtggagttc gtaattactt agaccgccag gtgatgcggc cgcgggtcag
    19381 atcgtaagga ctcatctgta ccgtgacctt gtcgccgggc aggatgcgga tgaaattcat
    19441 gcgcagcttg ccggagatat gggcaagaat ggtgtggccg ttgccgatgt ccacccggaa
    19501 agaggtgttc ggcagggatt ccacgaccgt gccctccagc tcgataacgt cgtctttcgc
    19561 catataagtc cctccttaaa tgtcgtcggc aagagtcagg atttccggct cgccgtctgt
    19621 gatgagaatg gtgttctcat aatgggcgga caggcttccg tccacggtga cgaccgtcca
    19681 tttgttggac agtacgcgca ccgccggtcc gccggcgttg atcatcggct caacggccaa
    19741 tgtcatgccg ggcaggagcc ggggattcgg cctgcgctcc ctgcgatcgg gaacgtagtt
    19801 cgggacttcc ggatcctcgt gcattttggt accgatgccg tggccgacat agtcgcgcac
    19861 aacgccgcag ccgtggcttt ccgcgtactg ctgcaccgcg gcggagatgt cgttcagccg
    19921 gtttcccgca cgggcaaact tgatcccttc aaagaagctc tgccgcgtga tgcggatgag
    19981 ctccagcgct tccggcgacg cctccccgac cgggaaagtg ccggcgcagt ctcccacata
    20041 gcccttatag gtggctccca cgtcgatgga gacgatgtct ccgtttcgca cgctgcgctt
    20101 tccgggaatg ccgtggatga cctcgtcgtt cacggaaatg cacgcgctct tcgggaagcc
    20161 gccgtagccg agaaatgtcg gcgtggctcc acaggacagg atgaacttat ggatctcttt
    20221 gttgatctct tcggtggtta cgccgtcccg cagcatctgc cgtccgacgc ttcgggcagc
    20281 cgctgtgatc tttacggctt cgcgcatccg ctcgatctct cgctgtgatt tgatcgtaat
    20341 catcattcaa cgatccccag agagcggaaa atctcacggc ttacatcctc gatgctgccg
    20401 acgttttcca cactgtgaag cagccccagc ttttcgtaaa agcccttgag cggctcggtc
    20461 tgttcatggt agacatgcag acggtcgcgc acggtctcgg gcgcgtcgtc cgggcggacg
    20521 atgagcgccg tgccgcaggc gtcgcagatg ccgtcctgct tcggcggatt cacgcggacg
    20581 tggtagctcg cgccgcagcg ggggcagacg cgccgccccg ccatgcgctc tacgatgtcc
    20641 tcgtcggaaa cctccaggga aagcacgctg tcgagctcaa tgccgtgagc gataagcgct
    20701 tccgcctggg ggatcgtccg gggcatgccg tcgaggatga atccgttgac acagtcgggc
    20761 tttgagagcc gttcctgcac gatgtcgatg atcacgttgt ccggcacgag ctggcctttg
    20821 tcgatgtacg acttggccgc gagacccacg ctcgtgccat cgtggatggc agagcgcagg
    20881 atattgccgg ttgaaatggt ggggacaccc aatttacggg ataggatgtc cgactgggtc
    20941 ccctttccag cgccgggggc gcccagcaaa gtcagcttca tgtcgggatc tccttattca
    21001 aggaagccct tgtagtggcg catgagcatc tggctttcga gagcctgcac cgtctcgatg
    21061 gcaacgccga ccacaatgat gatcgaggtg ccgccgaggg agatgcccgt atcggcagcg
    21121 gccttggagc aggcgctgat gatgatgggc acgcaggcga tgatgccgag ataaacggcg
    21181 ccgaacaggg tgaccttgtt caggaccttc tgaatgaact cgctggtcgg cttgccgggg
    21241 cggtagccgg ggatgaatcc gccgttcttc ttcaggttgt tcgcaacttc gacggggttg
    21301 aactggatcg tcgcgtagaa gtaagcgaag aagaggatca gcaggaagta cacgatcgcg
    21361 taggggatcg tgttgctgtc aaacgtgcgc atgaaccagc tgtcctggct gccgccgcag
    21421 agcaggatga tcgtgggggg cagggacgcg atggactgcg cgaagatgat gggcagaacg
    21481 ccggacatgt tcaccttcat cggcaggtac gtggcctgac cgccgtacat cttgcggccg
    21541 acgacgcgct tggcgtactg gaccgggatg cggcgctcgg catcgttgac gatgacgatc
    21601 atcacgatca tggcgagagc accgatcagc atgaggatcg tggcgaccca ggagaggtcg
    21661 tggttgatcg ctttgctgac gaggttacca aggccctgcg ggaagcggct gacgatgctc
    21721 gcgaacagga tgatggagat accgtttccg atgccgaatt cggtgatctg ctcacccagc
    21781 cacatgacga gcgcggagcc tgcagtaaag gtcatcacga tgacgatggc cgcccagaca
    21841 gcgggagcct cggacacgat gataccgttg cggctcataa gcaagtagta gccgtaaccc
    21901 tggagaaggc cgatgccgac cgtggagtaa cgggtgatgg cagcgatctt cttcttgccc
    21961 tcttcgccgc cctctttgct cagccgctcg agcgccggga tagcgatgca caggagctgg
    22021 atgataatgg aggcgttgat gtagggctgg atgctcagag caaagatggt agccagggag
    22081 aacgcgccgc cggacatggt gttgtaaagt ccgaggacgg tgttcttcag ctgggcaaag
    22141 taggtggcaa gaatgttggt gtccacataa ggcacgggca cggcattgcc gagccggtaa
    22201 agaagaacga tgaacagcgt gaagaggatc ttccggcgca gctcagggat cttccatgcg
    22261 ttacggaaag tctggaccac cttacaccac ctcggccttt cctccagcgg cttcgatctt
    22321 ctccttggcg ctggccgaga aggcactggc cttgacggtg agcttcttgg tgatctcacc
    22381 gctgccgagg atcttcacgc cgtggacaca cttggacagg atgccctcgg ccagaaggac
    22441 gttcgcgtcg acaatggcgc cgtcctcaaa gtggttgaga gcagagacgt tgatggactc
    22501 gagcggtttg gcaaagatgt tgttgaagcc gcgcttcggg atgcggcgcg ccagaggcat
    22561 ctggccgcct tcaaaaccga tgcggacacc gccgccggag cgagctttct ggcccttgtg
    22621 gccccgaccg ccggtcttgc cgttgccggt gccgatgcct cttcccttgc gcttggcaac
    22681 gtgggtgctg ccggcggcgg gagaaagttc attcataaac atacgttggg cctccttatt
    22741 cttccgtgac cttcagcaga tagccgatct gggcgatctt gccgcgggtc tgggggttgt
    22801 caggctgggt cgtctcgttg ccgatcttgt aaagacccag ggagttggca gtggcaatgt
    22861 gcttgtccag tctgccgttc aggctcttga cgagcttgat cttcagagtt gccataccgt
    22921 tatcctcctt acgccttcag ctcgtcggcc gacttgccac ggtaaacggc gacctgctcg
    22981 gcattgcgca gagacagaag gccttccatg gtggcggcga cgacgttgtt gggattgttg
    23041 gagcgcaggc tcttggcacg gatgtccttg atgccggcag cttccatcac ggcacgcaca
    23101 gcgccgccgg cgatgatgcc ggtaccgggg gcagcgggct tgatcagcac gcggccggcg
    23161 ccgaaagcgc cgagaacttc gtgcgggatc gtggtgcccg tcagcgtgac ggtgtgcatg
    23221 ttcttcttcg catcttcaat tcccttgcgg atcgcttcgc tgacttcgcc ggccttgccc
    23281 atgccgaagc cgaccttgcc cttgccgtcg ccgacgacca tgagcgcgga gaacttcata
    23341 atgcggccgc ccttgacggt cttgctgacg cgattgaggg aaacgagctt ctcgatatac
    23401 tcggagcttt cctcccggcc gcggcggttg tcgcggcgat cgttaccata tgccataatt
    23461 cgtttcctcc tcccgcttag aatttcaggc cgccctcgcg ggcagcttcg gccagagcct
    23521 tgacgcgtcc gtgatagata tatccgccgc ggtcgaagac cacttcttca atacccttcg
    23581 caagagcgcg ctcggcgatc ttggagccga tcttcttggc ggcttccacg ttgccgccgt
    23641 tgccttcaaa gtccttctcg acggaagagg cagannnnnn nnnntcgtcg atgacctggg
    23701 cataaatgtt gctttcgctc cggaacacgc acaggcgcgg acgctccggc gtgccggaga
    23761 tcttgccgcg gactctcgcg tggcgctgct tgcgctgagc ggcagtattc ggtctgttaa
    23821 ccattcaggc gcacctcccc ttatttcgca ccggtcttgc cttctttgat acggacaacc
    23881 tcgccctgat agcgaatgcc cttgccctta tagggctccg gcggacgctt catgcgggta
    23941 tccgcagcaa actggccgac cttctgcttg tccatgccct tgatgacgat gtggttggca
    24001 tcgggaactt cgatttcgat cccctcgatc tcgggaacga tgacctgatg gctgaagccg
    24061 aggttcatca cgagattctt gccttccttc tgcgcacgat agccgacgcc ctggatctcc
    24121 agagtcttgg agaagccctg ggtgacgccg atgaccatgt cgttgaccag cgtacgggtc
    24181 agaccatgga gcgcgcagtt ctgcttttcg tcgttgggac gcttgacgcg gaggacgccg
    24241 ttctcgatct cgatgagcat ctgcggggcg acattgcgct ccagcgtgcc cttgggaccc
    24301 ttaacggtga tgtggttgcc ctcgcccacg gtgatctcaa caccggcggg gacggtaatg
    24361 ggagctctac cgattctaga catgttctct ctcctccctt accatacgaa cgcgaggact
    24421 tcgccgccga catgctcggc acgagcctgc ttggcggtca tgacaccctt ggaagtggaa
    24481 atgatggcga tgcccagtcc cttctggacg cggggcagct cttcggcgcc ggcgtacata
    24541 cgcaggccgg gcttggagac tctcttaagg ccctggatgg ccttctcctt gccggggaga
    24601 tacttcagcg tgatgcggat gatgccctgg gttccgtcgt caacgagctg ataggacttg
    24661 atgtagccct cgttcagcag gatctcgacg atcgccttct tcatcttgga ggcggggaca
    24721 tcgacggttt catgcttggc ggtgccggca ttgcggatac gtgtgagcat gtcggcaatg
    24781 ggatcggtga tctgcatttg ggtatatcct ccttattaga gttaccggtt ttacccggtt
    24841 caggcagcga ggttccgcgt caatacatga ttgaccgcgg cctttcgcta tcctttacga
    24901 aatttgtcgg ccgtgccaac tgacctatta tagcaaagat ccgctgacac ggcaagtccg
    24961 ccaaggcctg aaattcaatt ttttattgaa tttcaggcgc ttgacggagc aatttgcaat
    25021 taccagctgg ctttcttcac gcccgggatc tggcccttgt aggccagctc gcggaagcag
    25081 atacggcaga tgccgtagtc gcgcagatag gcgtggggac ggccgcagat cttgcagcgg
    25141 ttgtagcggc gggtggagaa cttagcagga gctgcctgct tgagcttcat ggaaagtttc
    25201 gccatttttc ttctcctcct tagtcgttca tgaacggagc gcccatgagc ttgaggagct
    25261 cgcgagcctc ttcgtcggtg ttggcggtgg tgcagatggc aatgttcatg ccgcgcagct
    25321 tctcgatctt gtcgtactcg atctcaggga agatgatctg ctcgcggatg ccgagggagt
    25381 agttgccgcg gccgtcgaac gcatcggcgg agatgccgcg gaagtcacgg acacggggca
    25441 gagccacgtt gaacagacgg tccatgaact cccacatacg gtcctgacgc agcgtgacct
    25501 taacgccgac cttcatgccc tcgcggagct tgaagttcgc gacgctcttg cgcgcgacgg
    25561 tggcgacggc atgctggcca gtgatggcgc tgatgtcctt gacgacggat tccagcatct
    25621 tggcgttgtc cttgcagtcg ccgcagccga cggacacgac gatcttgtcg atgcggggga
    25681 tctgcatgac gctcttgtac tggaacttct gcatcagagc gggagcgact tcctcattat
    25741 actttttctt cagtcttgca acgtaagcca taatattctg tcactctcct ctctcagatg
    25801 gcggcgccgc acttcttgca ggcgcgcacc ttttcgccgt cgataacggt gtggcccacg
    25861 cgggtgggct tgccgcactt cgggcagacc agcatgacct tgctggcgta aatgggggtc
    25921 tcgcgctgga tgatgccgcc ctcttcgccc tgcttgcggg gcttctgatg acgggaggcg
    25981 acgagcacgc cctcaacaat gaccttgccg ttcttcgggt cggcggagag gactttgcct
    26041 tccttgccct tgtcgcggcc ggacagaacg acgactttat cgtctttacg aatcttcatt
    26101 cccatgccct cctcaaatga cttcgggagc cagcgacagg atcttcatgt actctctgtc
    26161 gcggagctcg cgggccacgg gcccaaagat acgggtaccc ttcgggttgt ggtcatctgc
    26221 gccgttgatg agaacagcgg cgttctcgtc gaaacggacg taggtgccgt ccgcgcggcg
    26281 gacgcccttg gtggtgcgga cgataacggc cttgaccaca tcgcccttct tgacgctgcc
    26341 acccggggtg gccttgcgga cgctggcgac gacgacgtcg cccacgttgc cgtacttgcg
    26401 cttggagccg ccaagaacac ggatggtctt gagctccttg gcgccggtgt tgtcggcaac
    26461 cttcagataa gattcagcct ggatcatttc ttcggcgcct ccttacttcg ctttttcgat
    26521 gatctcgacc acgcgccaac gcttgtcctt gctgaggggg cgggtctcca tcacacggac
    26581 tctgtcgccg atgccgcact cgttgttctc gtcgtgcgcc ttcagacggt acgtccggcc
    26641 gatgatcttc ttgtacgtgg ggtgagccac gcggtcttcc acggtgacaa cgacggtctt
    26701 atccatcttg tcggagacga ccttgccgac gcgcatcttg cgggaagaag ttctaatttc
    26761 actcattttc ggttacctcc ttcactgctc gagctgcttc tgacgaagca cggttttaac
    26821 tctcgcgata tcgcggcgga cgttggaaat cttgatgggg ttgtcgagat ggttggtggc
    26881 atgctgcata cggagcatga acaggtcctt cttcaggtcg gcgagcttct tgtcgagctc
    26941 ttcgacggac aggttacgaa tctcattagc cttcatcagt tttcaccacc attctcggtc
    27001 tcggcggcct cacgcgcgac gatcttcgtc ttgcagggca gcttatggct ggccaggcgc
    27061 atggcctcgc gggcggcatc ctcgggaacg ccggcgatct caaagagaac gcggcccggc
    27121 ttcacgacgg caacccaata ttcgggagag cctttaccgg aacccatacg ggtctcggcg
    27181 ggcttctggg tcacgggctt atcggggaag atcttgatcc aaacctggcc gccgcgcttg
    27241 gtgtagcggg tcatggcgac acgggcggcc tcgatctggt tggccgtgat ccagcagggc
    27301 tcggtagcca tcaggcccca ttcgccgtag ttgacgacgt tgccgcgggt ggctttgccc
    27361 ttcatacggc cgcgctgctg gcggcggtat ttgactctct taggcattaa catcaggaat
    27421 tacctccttc tttgggagca gcgggacggc tgtagccctg gcccgccgga cgctggccgt
    27481 tgttggggcg gttgtagccc tggcccgcgg gacgctggcc gtagccctgg ccgggcgcgc
    27541 ggttgtcgcg gttgaagccc tgaccctggc cgcggttgtc gcggttgtaa ccaccgcggc
    27601 gatcgtcacg gcggccgccg cggcggtcgt cacggcgacg ctcgttgagc ggcttgctgg
    27661 tgtccatcac gcgcggcgtg gtgcgcagcg tggtggtgag gatctcaccc ttgtagatcc
    27721 agaccttcac accgatgcgg ccgtaggtgg tcgcagcctc ggcaaagccg tagtcgatgt
    27781 cggcgcggag agtctgcagg
//
LOCUS       DAWAAE010000009        26858 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_783_length_26858_cov_3.490579, whole genome shotgun sequence.
ACCESSION   DAWAAE010000009 DAWAAE010000000
VERSION     DAWAAE010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 26858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26858
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_26858_cov_3.490579"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12021..12030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16356..16365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25916..25925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5427 a         8361 c         7836 g         5204 t
ORIGIN      
        1 gtccatagga accgcgcccc cgggtttttt atgccgccgt gttctgccgg tgtttcttcc
       61 cgccgagctc gctcacgagc gctgagccga agatcagcac cgcgccgaga aggccgatcg
      121 ccgtcagccg ctcgtgcagc agcagcgccg agaaaaagag ggaggacaca gggtcaatgt
      181 agctgagcac cgccaccgac tgcgcgggga gcttatttac attcccgaag tacagcgcgt
      241 acgcaaggcc gctgtgtacg atgccgacaa tgagcacaag gacgaccgtg cgcgcatcga
      301 gcactaccgc ggaaaaatcc tccgtgagca gcaggtacgg gatgagcacc gccgccgcgg
      361 agagaagctg gacgatcgtc ttttcgtagg cgtcctctac ctcgatcttc ttattgatta
      421 ttacggtcgc ggcatagagc accgccgcag ccagaccgaa ggcaatgccc ttcgcgtcct
      481 gcgcgccggg cggcgcgccc tccgccacgc cggagacgaa caccatgccg aggatcgccg
      541 cgagcgcgca gagccccttc tttacgctga gcttttcacg gaaaaggacc ggcgagagca
      601 ggatcacgat cgtcggctgc atgtaaaagc acatcgtcgc caccgccacg gtggtgcagt
      661 tataggcttc aaaaaggagg atccagtcaa cgccgatgat cgcgcccgaa acgataagca
      721 gcgccagctg ctttttttcg gccttttcca tccggtgatg cttcaccgcg acgaagatca
      781 gcagaaacac gccgccgagc agcccgcggg aaaacgcaag cagccccgac gaaaacggga
      841 tattccgccg gaagatcgcg atcgtgccga agatcagcat cgcgctgcac agggaagcat
      901 aggccttgcg gttttctttc atggtcccgt atcgctccta ttcttttccg ccgttcggaa
      961 cgtcagctcc gtcatggcga tcatgaaaac ggcaaacacc agaagatacg ccggaaggat
     1021 cgaaaagccg agcgcgctgc ccagcaggcc gaagagcggc ggcgcgaacg tcgagccgac
     1081 gtacgcgccc gccatctgta tgccgatgat cgcgccggag ctttccgccc cgaagtttgc
     1141 gggcgtggag tggataatgc agggatagat cggcgcacag ccgagcccca tcacgacaaa
     1201 ggcggcaatg gcggcggtat gcgaatgtat cgggagcgcg agtgccgcga tgccgcagaa
     1261 aagcacgccc gtgccaaggc ggatcatttt cctgtctccg agacggtcgg cggcaaagcc
     1321 gctcacgaac cgccccgcgg tgatgccgat ataaaagagc gcagccaacc ccgcggcgtc
     1381 ctccgtggga agccccttga ccgcgacaaa atacgtgctt gcccactgca tcgccgtggc
     1441 ttccgcggcg cagtagccga aaaagcccgc gagaagatac ggcacgccct ttattttccg
     1501 cgcacgccga cgctccgccg tttttcctcc ggcgcggtgc cgccggcttt tttccacaca
     1561 ggcagcgtca cgagcaaaag cgccgcgata cagagctgca cgagcgccac ggcgcggtag
     1621 ccgtcgttcc atgtccggtg cgtcagcgcc cagctcataa caaacgggct gacgatcgtg
     1681 ccgacgcccc agaagcagtg cagccagctc atgtggcgcg ccttataatg cagcgccaca
     1741 tagttattga gcgccgcgtc gatcgcgccc gcaccgagac cgtacggcac ggcgaaggcg
     1801 agcagcatcc agaacttcgt ggaaacagaa aagccgaaga gcccgagcac cgtgaggagc
     1861 acgctcgcgg ccgtcacgcc gccggtgccg agcttccgcg tgagcttatc cgacagaagg
     1921 ctcgaaacga tcgtcccgca ggagatcacc atggagacaa cgcccatgta cgacaccgga
     1981 acgccgagct ccgcgtgcat gaccggccag ccggagccga gcagcgaatc cggcagaccg
     2041 aggctgataa acgcaagata gataagtgcc agcagcagcg aatacatatt ttataccacc
     2101 tggacggcgc agtttttata ccacccggac ggcgcagccc tgcttttcgg tcacatagcc
     2161 gatgcgtgcg gcgtgcggta ccttatcggt gagctccgcg agcagcgcgg gcgcgtcctt
     2221 ttccgccacg gcgagcagca gtccgccgga ggtctgggga tcaaagagca cgtccatcat
     2281 cgcgcgcgtg acgttcggcg cgctctcgct gaagggctcg gcaaaattgc ggttgcggta
     2341 catcccctcc gggaggatgc cgatctcggc aagcgagagg acctccggca ggagcggcac
     2401 gttcgcgctt tcgatcacgg cggtcacgtt cccgccgttc gccatttcgc ccgtgtgccc
     2461 catgagaccg aagccggtca cgtcggtgat gctgtgcacc tcgtactttc cggcgatctc
     2521 actcccggcg cggttgagct ccgccatctg cgcgacggct ttggcgtagg tctcctccga
     2581 taccatgtcg gccttgttcg ccgtggagat gatgccggtg ccgagcgctt tggtgagaat
     2641 gagcacgtcg cccgcgcgcg gcgtgcagtt caaccgaatt ttatccgggt gggcaaagcc
     2701 cgtcacggaa aggccgtatt tcggttcggg atcgcggatg gtgtggccgc cggagatgat
     2761 gacgccggcc tccgccgcct tcgtatagcc gccgcgcagc acctcgcgca cggcctcgtc
     2821 cggcatctgc ggcgatacgg ccatgatatt cagcgcgagc ctcggctgcg cgcccatggc
     2881 ataaatatcg ctgatggcgt tcgccgcggc gatctggccg aacatgaagg gatcgtccac
     2941 gatcggcggg aagaagtcca gcgtctccac gagcacgagc tcgtccgaga tcttataaac
     3001 gcaggcgtcg tcgctcgtgt cgtagccgac gagaaggcgc tcgtcataca ccgagggcag
     3061 acccttcaga agctttgcga gcgtgcccgc gccgaccttc gcgccgcacc cggcgcagct
     3121 tgcgagcttt gtcatgcgaa tctcattttc catagtcttc tcctcgtttc tccggttttc
     3181 tcgccgttcc gacggcggag gtgagcatca gcaccgcttc gagcacgccg ccgccgacag
     3241 ccagcgcctt gtcggacgcg gtaaagcagt ggaaaagccg gcagcgggga tcgacgtccg
     3301 cgctcttaaa gccctcgcgc ggaacataaa acccttcggg cagtatgccg cggagcgtac
     3361 cggtgatctt tgcgcacagc ggctcgccgc cgacggcggc gacagtctcc cccgcctcga
     3421 ccgtgtcgcc gatctgccgc agggcgtgga actctcccgc gcagggcgag tgcatcacgc
     3481 gctcgatcgt atagccgccg atgttgccgg gaacgccggt gttggggacg gcactgcccg
     3541 accagatcac gcggccgagc gtgtggccgc gcatcgtctc cacaacggcg tggcagtccg
     3601 tcccggcggt gaagcccggg ccgacgccga cgacgcccgg agcgtcggtg atggcggtgc
     3661 cgagattttc cttggcgagg atcgcgtcca cgatcacatc cggatggagc tctttcgcgc
     3721 tctccgcctt cggatcgacg atcaccggca ggatgtcctc ttcaagcagc cgcagcgcat
     3781 cggcagcgtc cgcggcaaac cgggcgtgca cctcctcgac cttcgtttcg ccgcggtata
     3841 ccgcctcgga aaagcagacg gtgcggcgga tggacgacgg cttcggaaga tcggtcatca
     3901 caacgttaat ccccgcgcgc cagagccgca gcgcaatgcc ggaggcaatg tctccggcgc
     3961 cttttatcat tacgagcata gcctgttccc cttagaaatg gttctggatc cagtataata
     4021 tggaatatcg ggttgtcaag ggtattggct cttgactttt tatcgaattt ttcatatgat
     4081 atccatataa tatcgatata caaagaggaa gtgctctatc atgacgctca agcagctcga
     4141 atactttctt gccatcgccg aaaacggcag catcaccaaa gccgccaatt cccttcgcat
     4201 ttcccagccg ccgctgagtc ttcagctcaa ggggctggag gaggagctgg gctgccagct
     4261 cttcatccgt gacaagaaaa atatgatcat cacgcccaag ggcattctcc tgcgcgagcg
     4321 cgcccgcgag atcatggaca aggtaaacga aacgctgctg ctcatgcagt cggccaacga
     4381 ggaggaacgg tacaccatcc acctcggcac gatctcctcg atctgcaacc gcgttctgcc
     4441 ggataaggtc ctcaagctca aggagcgcta tccgcacgtg gagttcgtcc tccacgaggg
     4501 cagcacgacg agcattctcg atatgctcgc cgacggcacc gttgatttcg gcatcgtgcg
     4561 cgagccgttc aacgtaggcg cttacagctc ccgtcccatc cgcgataccg cgctcggcga
     4621 gggcggccag gactacttta ccgccatcgg caagccgaac ttcttccccg ccgacgatac
     4681 gcagatcgtg gcgctcacca gtctgcagga cacccccatc attatccacc ggcggtacta
     4741 cgatcttttc accaacgcct gccgccgtca gggctttatc cccaacatca tctgccagaa
     4801 cgacaacatt gcctcctcgc tgagttgggc ggcatccggc atcggcgtgg cgatcgcgcc
     4861 gtatacctcc gcgatccaga actccgatcc cacgctcgtc atgaagcgca tcgacagccc
     4921 ggagatcgtc tccaaggcgc acctcatctg gaaccgcagc gccacgctca cgcctgagca
     4981 gaaggcgttc gtgcatctgt tcgactgata gtatcccata cgaaatgaga gccggttttc
     5041 cgcggaagac cggctctcgt tttattttct tgctttaata cccgtgatcg acgtgctgcc
     5101 acgtaccgcc gttcgtgcgg acaaggatcc acatccagcg cgtgtaggtc tgccccgggt
     5161 tgagcgaggg gctggcgccg gattcgtcca catcaaaggt ggacgtgagg acaagcacct
     5221 cgtcggcatt gttgcggtcg gcccagtcct ggtgctcgcg cgacacgtcg tccccggcgt
     5281 aggagatctc cgtgagcgtg cagcccttcc agctgcggga aaagtccttt tttatgacgt
     5341 cgatagcggc gtcaatgtca ttttgcgtat acatttcaga agcgacggcc ttggtctgca
     5401 cgtccttcac attgccgccg caggctgcaa gagcgagcag cagcacggcg gccagaagca
     5461 gggatagaaa gcgtttcatt ttatatttct cctttcgtag agggcaaacc cttccgtcaa
     5521 ggcctttggc tttgccgccc ccctgtcagg ggagacaagc cggtttgctg caaacgcact
     5581 cggcctccct aacagggagg ctggcgcggc gcagccgtga ctggagggtt ttctccgtat
     5641 agactcagac gccccggcgt gtcttttggt tcacctcatt taaaccgcgc gaaaaactcc
     5701 gcgagcggct gcatgacggc gagcagctcc gtgaccgccg tattgagtcc ttccacatcg
     5761 agcttttccg cggcgtcgtt cacggcggtg agcgcttcct ccgcggcggc aaggctcttc
     5821 tgcgcctgcg cgagcatgtc gccggtgctc tgctcgagcg cggcccagtc caccttctcg
     5881 agctgcgcgg agatcgtccg cagcgagtcg agcgtatcgg caagctgtgc cttttcaagc
     5941 tccccggtca ggccgttcac ggtatcgacg agccgctgcg catcgagcgc atccagctcc
     6001 gccgtcacgc tgtcgagccg cgagacgatg tgctcgatgc gcggggcgta ccgccgcaca
     6061 ccgacaaagg ccagcgccga gcttgccgtt acgacgatga gcagcagtat gcatacgacc
     6121 gccgtcacct tctgccagtg cagacgtttt ctttccagtt cctccatcgg aagccctccc
     6181 tctttttatt cctcgccgca gagatagaga tacatgagct tgagcgtcgc ctcaagcccc
     6241 tttatgtgcg tgcgctccat accgtggctc gcacagacgc cgctgccgat gagcgcgccc
     6301 ttgcagtctg cccccgcgtg ccacgcagcg ccgacgtccg agccgtagaa cgggaagata
     6361 tccacggtgt acggcagctc atgcttcttt gcgagcgcga tgagccggtt cgtgagctcg
     6421 tagtcatacg gtccgccgga gtccttcgcg cagatggata cggcgtattc gctgcctgca
     6481 agatcaagcc ccacgcagcc catatccacc gtgacaaact catcgatatc gcgcgcgagc
     6541 gcgctcgcgc cgtggccgac ctcttcgtat acaacaaagt atatgcgcgt cggatggcgg
     6601 aacgccgcct ttttctcgct gcaccagcgc agcagaagaa ggagcagcac cgcgctcgcc
     6661 ttgtcgtcga taaaccggga cttcagaaag cccgatccgg tcatcgtgaa tttcggatcg
     6721 atgaacacaa aatcgccgtt atcgatgccg agcgccctga catcctcggc gcttttcact
     6781 tcctcgtcga gccggacctc gagatggtcc gcatcccgcg tcatcgtcgc ggcgtcgggg
     6841 taaacgtgca ccgccggggc gagcgagagg atcgtgccgc tgtagcgtct gccctcgcgc
     6901 gtgagcaccg tgcagtattc gccgtcaagc gtcgggcaga tcggcccgcc gactttcgtc
     6961 agggcgagcg ttccgccgct cttcacgctg cgcaccatca tccccagcgt atcgacgtgc
     7021 gcggaggtcg ccacagtctt gctttcgtcc agccccggca catccacttc gagcgtgccc
     7081 ttgttgtgga catgcgtcgt atagccgagc tcttccacat aacttttgat gagcgcgatc
     7141 gcatcggccg tgcagccgga gggcgagtcg gtcgtgaaca cggcgcgggc atattcttca
     7201 aattgacggc ggtcaaactc gatcatggaa agttcctctt ttctatgaga taggcatatc
     7261 ataagccccc ggcgcgcgtt tgtcaaaatg tacatatatg ccgcttctgt cctattgaca
     7321 ggcacggttc acggcggtat acttgtatca agttttttat ataaattttt ttaactattc
     7381 acataaaagg aggccacagc catgggacag gtcgccatcg tgaccggcgg cagctccggc
     7441 atcggcgcgg cggcagcgga atatcttgcc gcgcacggct gcacggtgta cgagttttcc
     7501 cggcatgaaa aaagcggcgg cagcgcgtcc gttcaccatg tgacggcgga cgtgacggac
     7561 gaagcgtccg tcgccgcggc ggttcagtcg gtgctcgacg ccgaggggcg catcgatata
     7621 ctgctcaata acgccggttt cggcatttcc ggcgcggcgg agttcacgtc ggtagaggag
     7681 gccaagcgcc agctcgaggt caatttctgg ggcatggtac gcgcaacaaa ggccgtgctg
     7741 cccgtgatgc gcgcgcagcg cggcggaagg atcgtgaaca cgagctccgt cgccgccgtg
     7801 acgcccattc ccttccagac ctattattcc gtttccaagg ccgcaataaa ctcgtttacg
     7861 atgtccgtcg gcaacgaagt gcggccctac ggcatccgca tcagcgccgt gatgcccggc
     7921 gacacccgca ccggctttac cgacgcccgc gcgcgcagct ttgccgggga cgacgcctac
     7981 ggcgggcgca tctcgcgcag cgttgcaaaa atggagaagg acgagcgcgg cggcgtttcc
     8041 gcctcggtcg tcggcgcggc gctggggcgc gcggcgctct caaagagccg gaaggtgttc
     8101 tgcaccgtcg ggtttgtcta tcggctcgtc atttttctca tgcggctgct gccctcgacg
     8161 gcggccaacg ccattctcgg caaaatgtac gccgaataaa accaccccga aaggagtttt
     8221 cccatgaacg aacaggaagc caaacgcctt tgtgcgctgc gcgagcttgt gcgcgaaacg
     8281 ttcgcgcaga cgctgatgat ccgcaccgac accatctccg acaccgccga cttcttcgac
     8341 gagctcggcg gcgacagtct cgacagtctt gatgtcgcgg tgcatcttga ggagatctgc
     8401 gggcagcgcg tgccggagga ggaatacgct ctctgccgct gcgtggagga cgccgctctc
     8461 ctgctgctcc gtctcgagca gggcggcgcg ccggaagccg cgccggagga gaaaaagacc
     8521 gtaacgccga tcacgcgctt tgaggatacg ccggaatacg ccgcctttca gcagcgtctg
     8581 cgcacggcgg acgagaccgg caacccgtac tttctctgca acgattcgcc gctgcgcgat
     8641 acctcgctcc acgacgggcg cgagatcttg aatttcagct cctacaatta cgccggtatg
     8701 tccggccgcc cggaaacggt caaggcagcg caggaggccg ccgcgcacta cgggtgcagc
     8761 gcctcggcca gccgtctgct tggcggcgag aagcccatcc acaaaaagct ggaaaatgcc
     8821 atcgccgcgt ggaagcacac cgaggacgcc gttgttctcg tcggcggaca ttccacgaat
     8881 gtgacgatcg tcggcaactt ctgcggcaag ggcgatctca tcatctatga cgcgctcgcg
     8941 cacaattcca tccacgaggg cacgcgcctc tccgatgccg aaagccgccc cttcccgcac
     9001 aacgacaccg ccgcgctcga gaccattctc cgctcctgcc gcgataagta cgccaaggtg
     9061 ctcatcgtcg ccgagggcgt gtacagcatg gacggcgaca ttgcccccgt gccggagctc
     9121 gtgcgtatca agaaggagta cggctgcttc ctgatgatcg acgaggcgca ctcggcgtgc
     9181 gtgctcggca ggaccggcgg cggcgtggac gaatacttcg gtcttgccgg ggacgatatc
     9241 gacatgaaaa tgggcacgct ctccaagggt ctcggcacct gcggcggata tcttgcgggc
     9301 agccgcgcgc tcatcgagta tctgcgctac aacctgccgg ggttcgtgtt ctccgtgggc
     9361 atttcgcccc cgctcgccgc ggcgacgctc gaagccatcc gcctcttgca ggaggatccc
     9421 tccattatgg aaagcctgca ccggaacatc cgctgcttcc gtgaggaggc gaaaaagcgt
     9481 catttcaaca cctgtcttgc gggcgagacg gcgatattgc cgatcctcgt cgggcgcgac
     9541 gaggacgcct tcgcgctctc gatggcgctt ttggagcgcg gcgtgttcgc gccctcggcg
     9601 gtctatcccg ccgtgccgcg cggcaaggcg cggctgcgct tctgcgtcat ctccgagcac
     9661 aagccggagc agatcgtaac ggcgctcgac accctgcagg aggtcgccgc cgagctcggt
     9721 atcaaactgc ctgaataagc aatcctgccc gccgtgcgct tgtgtgtacg gcgggtttct
     9781 gttataatca cgtcatggaa atcccacgaa aggcggcggc agatatgctg gaaaaaatga
     9841 tccgctccca gacggcgaag caggagcagc agctcgagag ctttctcgcg cggcacgacg
     9901 cggcaaatcc gttcccggag gacgtgctcc tcacggcgga cgcggactac atgggcgatg
     9961 gccggccctg tcaccggatg gacatattcc gcccgcgcga ggcaaaggaa gccctgccgg
    10021 tgctcgtgaa catccacggc ggcgggttcc tgctcgggaa gaaggaggta aaccgtctct
    10081 tctgtgcgga catgtgccgc tgcggatttg tcgtattctg tcttgaatac ccgctcgtgc
    10141 cggatgtgga tatcttcacg gtgttccgcg ctctgactgc gggcattgag cgtgccgccg
    10201 cgctcgcccc ggactttggc ggggacgctt ccaggctctt cctctgcggc gacagcgcgg
    10261 gcgcgtatct ctgcgtgtat ctcgcggcga tgcgcaaaaa cgccgccatg gcgcgtaccg
    10321 ccggggtgcc gcagctttcc tcggacatcg cggcgctggg cctcatctcc ggcatgtttt
    10381 atggcgctta cttctgaagc aatttccatg ctaaaggaga aaaatgtaaa gagtgtgtta
    10441 ggtcagtatt gatcacattt tacctgaata ccatgatgca tttcgtggtg tagagggctg
    10501 ctacgcgcga gcaattgaag ctataaaaga gtgtgtcagc aaaaagattt caacaacgat
    10561 tggctttacg ccaagaattg gagggagtca ttgagttgtg cctacaatta ggcgtttctt
    10621 gtttcaatct atcacaattt gttcctgttg gaagaggttc agtagaatta gatatcccgc
    10681 ctattgcatg gaagcaaata atgcatatct ggaaggaaaa aaattaaaat ttggaaataa
    10741 gatgaatttc tacacacatg aggcgcagca gatccttttg gatgattcgc tttttgattt
    10801 gccatacttt acaggatgtc aggcaggcaa tggtgtttgc tgcatcaaag caaatggtga
    10861 tattcttcca tgtgttctct tggacattcc gattgggaat atccgagaaa acagcttttc
    10921 tacgatttgg ctaaatagcc caattcttga agagctaaat aatcgacaga aatattctga
    10981 accatgtgta cagtgtattt atgtggagaa atgcggtgga tgtagaggtg tagcatatgg
    11041 aagttttgga gattattttg ctccagatcc tcactgttgg ctagattcaa atggagaaag
    11101 gctgtgagtt ttgtgtgtga aacacatcaa gaatctatac ctatagaatt gctgaaagga
    11161 tatttttcta gaaacggttt tttgctttgc aatgagtgcc gtaatatgcc gtcgcttttc
    11221 aatgtaggag gcgactggaa tagcattgta caattaatag aagaaggtca agttttttat
    11281 agcaaattat ataaaggtcg ggtcacatat ttgtccagag aattctatgc gcaggttaaa
    11341 ccttatcgcc agcgtatgga gctgatttca agccatagta ggttaatata caatttactc
    11401 gataccattg ggtatgctaa cgcacaagat atcaaacgtt tattaaacct ttcaaacaaa
    11461 gtatatacgg acgcaatgca tgaactaagt caagagcttt taattaccgc aattcaacgc
    11521 gataaaacga taaaccaaaa ttggagctca ttttattggg ggacatataa gacatgggag
    11581 tctctacatc ctatatctga tatagaagta tcgaccagtc gacttaagca attactcgat
    11641 agattaattt ctgaaaaaga aattcataaa ttattacagt gagacgattt taaggaaata
    11701 agactatctc tttaatcagt ctcaacaagc tcctggtaag tcatacttat actgtatacg
    11761 acccgctttg acaacatcgg cattttcctc ccgaacatag tctatggccg cggttggcgg
    11821 catgcgccgt tccgggaata catcgacccc gaaaatccgt cgctgatcgc cgctctcccc
    11881 gccctgcttc ctcgtgacgg cgcgcggtga cttcctccgc cattacagcc gcgactttgc
    11941 cgcggcattg aagaaaaacg gtctggaggc cgagcttttc gacatggacg agccggaaaa
    12001 gctcgcccac gctttcgccg nnnnnnnnnn cggaaacggc cgcagcgcag cgcgccaacg
    12061 ccgccatggc ggattttctc aaggcgcata tataaacgaa aagccaaacg gcgcaacctt
    12121 tccgtccgcg tcgtttggct tattttttat aaacggacgt tacccaacaa aagcggcgtc
    12181 cctctcaaag gctcccttgt gtaaagggag ctgtcaaaat gatttcaaaa atcattttga
    12241 ctgagggatt gttgtaccgt cgaatcgata taagcgcaaa cctcctggaa atgataccgg
    12301 atttcgttgt tcggaatgcg aagcacagtc aagccgtagt ttttgagaaa agccgttcgt
    12361 tcggagtcat attctatggc ttctgcttct ccgtgttggg agccgtccaa ctcaataacg
    12421 agttttgctt cggcacagta gaaatccaca atgaatttcc ctatcacctt ttgccgcaaa
    12481 aagcggacag gatagccgcg caaaaaatcg taccataggt gtcgctcttc tttcgtcatt
    12541 ttcttccgca gctctttggc aaaaggcacc agttctttgt tatgcttata ctgcatggca
    12601 atccctccgt cacggcgtta tgcgccgcgc cacctccctt tacacaaggg aggctttgag
    12661 cggcagctgc atgacgatct cgtctccgtc gcggtcgatt tccacaaagc cgaagctctc
    12721 gtagagtttt cgcgcggttt cgtttccctc aatatagcag agagtgactt tatcgtactg
    12781 gccgtccgcc ttcagttcgt ccaaaatcgc ccgcactgcc gctttgccgt acccgcggct
    12841 ctgatacctc gcgtcgatga acatctgact cagatcgtag ctttgcagct ccggctcgtc
    12901 ataataaagc cccatgccga cggggagatc atccgcacag atcagaaacg cccggctgcg
    12961 ccgggcgcgg taagcatacg cccgcgccag caacttcgcc ctgtcggaaa caaacggctt
    13021 ctgcgcttcg gaaacggcca gaccgatgcg ccaatttccg gggtcaatat ccagaagatg
    13081 tatcattttc tttcctcaca caccggagag aacgaggctg ccaccgccct cgacggcgta
    13141 ttcgccgagc gcggcgggca ggaaatagga atcgccctgc cggaaccgat aaacgttccc
    13201 gccgcagagg atattcccct cgccgcgcac acacataagg tgcgtaaagc tttcgccgtc
    13261 cgtggcgagc gagatgcgcg ttttcacctc cgcgcggcgg acgcggaaat actcgcacgt
    13321 caaaacctcc tgcacgccgt ccggcgggca ggggggaagc gtatccgccg cgcggtgcat
    13381 gggcgcgtag ttcatcacct cggcagcgcg ttccagatgg agctgccgcg gctttccgtc
    13441 cgcgccgaga cggttatagt cgtatacgcg gaacgtcgta tccgaattct gctggatctc
    13501 cgcaacgaga atgcccgcgc cgatggcatg caccgtaccg ggacgcacga aaaatacgtc
    13561 gccggggtgt accgccacgc ggttgagcac ctcgcagatc gtgccgtcct tcgcccgcag
    13621 ccgcagctcg tccggcgtca cggcgcgcga aaagccgaga aagagcgtgc tctccggctt
    13681 acagtccacc acataccaca tctccgtctt gccctgctcg cccttttcgc ggcaggcgga
    13741 cgcatccgac ggatgtacct gaacggaaag gtccttttgc gcgtctatca atttgatgag
    13801 cagcgggaac ggcgcaatgc cgcagcgcgc gcccgccata ccgacgggat cggcgcgcag
    13861 gacatccgcc agactctcac cgcaatgctt gccggaatca atgtacgtct gcccgtcggc
    13921 gcgcacggag agctcccagc tctcggcgat aacgccctcg ccggttttat gatactcccc
    13981 gcgcaggcgc gtgccgcccc agagatagct ccgaaggcac ggcttgagcc gcagcggttc
    14041 gtatttcttc gtttcttcca tgatcgccgc ctccttttat ctttttaaaa tactctgttt
    14101 ttatcccgct ggcaagatac gaatggggct ggattattga aaaaaagtat tgtataatga
    14161 tttatacgcg gtctccgcag cggagaccgc cgggagaaat actatgagac atcttctccg
    14221 gcgcacggcc ggtgcgctgc tcgcgctttt tgttatcgcc gcctttgcag cgcctcatgc
    14281 gctcggcgcg ctttcccgcc cggcggtgga aaagcctccc tcgccggaag cggtgaatga
    14341 atgggaaagc gccgttttca ttgcccgtgc gaacggcgcg gccctcttcg cgtggcgcat
    14401 cgtaagctct gacggaacgg cgctctacga cgccgccgcg gcgccggagt attttccggg
    14461 tcttcgcgtg cagggcgcgg acaccgacac gctcacgctt ctcgacattc cctcctcgct
    14521 cagcggctgg caggtcgaat gcctgttcac cgacgatgcc ggggaaaaag cgctctcggg
    14581 acgcgcgtcg ctcacggtct accccgtcgg tccgaccgcg tcgccggagc cgacgcccac
    14641 gccggacgcg actcccaccc cggagccgtc tccgacgccg gagatcactc cctcgccgga
    14701 gccgaccgag atccccacgg cctcgccctc tcccgcgccg gaaacgcccg tgccgacggc
    14761 agctcccgcc cccgccgcgg ccgcgccgct ttcgcgccgc gcggtgctcg gtgtgtttgc
    14821 cggtctcgtt gcgctcgcgc tccttctcgg cgcgctctct ctgaaagtcc gcggcggcgc
    14881 gcacagcgcg caccggcata gataaaccgc gatcgattta tttttcatat ctagaaagga
    14941 gcacacactc atgggtcttc tcagaactgg catcggcacc gttaccggcg ttctttccga
    15001 tcagtggaag gaatatttct actgcgacgc gctcggcgcg gatgtgctgg ccgtcaaggg
    15061 ccgcaagcgc gtttccggcg ggtccaacaa cgggctggac aacatcatct ccaacggctc
    15121 cgtcatcgcc gtggcggacg gacagtgcat gatgatcgtc gagcagggca aggtcgtaga
    15181 cgtctgcgcc gagccgggcg agtacatcta tgacatctcc acggagccct cgctcttcgc
    15241 gggcggcaat ctctcctcga acatcaagca ggtgttccag acgatcggca agcgcttcac
    15301 cttcggcggc gtcgctccca aggatcagcg cgtgtactac ttcaacacca aggagctcgt
    15361 cggcaacaag tacggcacgc cctctccggt gccgttccgt gtcgtggacg aggccgccgg
    15421 catcgatctt gacatcgcca tccgctgctt cggcgaatac agctaccgca tcacgaaccc
    15481 gctgctgttc tatacgaacc tctgcggcaa tgtggaagcg gcctataccc gcgacaagat
    15541 cgacagccag ctcaaggccg agctcctgac cgcgcttcag cccgcgtttg cgaagatctc
    15601 cgccatgggc atccgctaca gtgccctgcc cggccacacg atggagatcg cgcaggcgct
    15661 caacgacgtt ctttccgcca agtggcgcga tctgcgcggc atcgagatcg tctccttcgg
    15721 cgtttccagc gtgaaggcga gcgaagaaga cgagcagatg atcaagcaga tgcagcaggc
    15781 caaggcgtac atgaaccccg gcatggccgc ggcgaatctt gcccgcgcgc aggcgaacgc
    15841 catgcaggac gccgcgaaga accagggcgg ctcggcaatg gctttcatgg gcatgaacat
    15901 ggcgcagaat gtgggcggct tcaacgcgca gaatctctac cagatgggtg cgcagcaaca
    15961 gcagcagacc gccgctccca cggcaaacgg ctggacgtgc ccgcagtgcg gcaccgtgtc
    16021 caccggcaag ttctgctcct cctgcggcac gaaaaagccc gaacccgccg ccgcgaacac
    16081 ctggacgtgc tcctgcggcg cggtgaataa gggcaaattc tgctccgagt gcggcgcgaa
    16141 gaagcccgcc ggcgtcccgc agtataagtg cgacaagtgc ggctgggagc ccgccgatcc
    16201 gacgcacccg ccgaagttct gccccgaatg cggcgacccc tttgacaatg gggacatcgt
    16261 gggctgatcc cgcctttgca tgaaaaagct cttatccatt gtacttatga ccgccgcgct
    16321 gctcgcgctc tgcgcctgcg gcgcggcggt ccccgnnnnn nnnnncccgc cgttttggag
    16381 tccggcacgc cgccggagac ttccgtttcg ccgtccgccg aggcgtcgga aacgccgacg
    16441 ccggagccga cggacgcgcc cacgcccacg ccgcccgcga caccggagcc gacggaagag
    16501 ccgacgccct ccccggaacc ggtgccggac tcgccgcttc cctcgtggga ggaattgcag
    16561 cgcacgctct atgaaacgaa cgcctactgc gcggcgctct atctcgggcg cagcagcgcc
    16621 gcatctcttt cggacgcact gccggagctt ctggcccaaa aggggctgga cggcatttcc
    16681 tatcttgcca atatttccgc ctcggcctgc gtggagcagc ccggcgacga ggtgttcatc
    16741 ctcatccccc gcggtgacaa ggtcttgtcg ctttataact acgttctcga aacgcaaaac
    16801 aattatgacg cctatcccgg cgcgctgctc tactcctccg ccgagcgctg cgccgtcgcg
    16861 gtgcgctgca acgaaagcga cgtgcgcccc aacgtcctcg cggtgttttc cggtctggac
    16921 ggcgagcaga gcttctcccc gcgcatcacg ctcgaggacg agacccttct ctccgcgccg
    16981 ggcgtgtaca cgctcattcc ccgctgacac tttttctcat caaaaaccgg ccttgctcct
    17041 ttttcaacgg agcaaggccg gtttttcttt cagttcatta cggtcgagcc ataggttttg
    17101 atgatctcgt tgagcatatc ggtgtgggta aagtccttgt tgtacacgat cctcgtttcg
    17161 cgcaccatgc tcagattttc caccggcagc gccgcgatct tgccctttcg cagctcgtca
    17221 agacacgcgc tgcgcggcag gatcgaaacg ccgagatcct ttcggatcag atccttgatc
    17281 gtggcgatgt tatcaacttc gagcgtaacg tcaaaggact gtatgctctc gccgttgctc
    17341 tcgagcgccg actcgaacag cgtccgcgtc gccgaggcgg gggtgcgcag tatcattttc
    17401 tgccgccgga gctccgcgag cgtgacggcc ccctttcgcg cgagcggatt gtccaccgac
    17461 atgacgcacg tgagatagtc cgtgtccaac agcatggagg agaagcgcgg atcgccgtat
    17521 gcgccgtcca cgatggcgag atccagctcg taattttcca gcatatcata aagattattt
    17581 atggtatccg taaaaaggat tatcttcaat ttgcccttct ggttactgta ccgagcgagc
    17641 gccgcggcgg tgagattgct ctcggacgta tgcgttatgc cgatgcggag cagcgagaga
    17701 tgacgctcgg cgttttgcag ctcggcaaat acggcggcgc gttctccggc tgcctgctca
    17761 ccgggaagga taagcgggtg cggaactatc tcggtctcgc gctgatcccg caggcgggcg
    17821 ttgcgatcgg tctcgccgcg atgggcgcgc gcacgctcgg cggcagcgcg ggaacggcgc
    17881 ttgaaacgat catcctctcg tcgagcgttc tgtatgagct catcggcccc gcgtgcgcga
    17941 agctctcgct ctatctttcg cattcgtatt ccaacaaact cgaggatctc acgcccgagc
    18001 cggagccgga cgagaccggc tgtccgaaaa gcgaagttga gcggctcatc gagcggatca
    18061 acgccatcca aagcgagctg cccgcgcacc gtgcggtcta ttatgaggac gaaaaggcgt
    18121 tttcggacgc cgccgaagag cactatgccg cgctctaccg ggcgctgccg gctgaaagga
    18181 gaaaacgatg atctttgcca tgcatgaccc tctcgccgat cttctcggcg aatggtcccg
    18241 cggactgaat accggctcgg tgctgctgcg catcgcgctg gcggtgctgc tcgcctcggt
    18301 gatcgggtac gaacggtcaa gcaaacgtca ctcggcgggg ctgcgcacgt ttgtgctcgt
    18361 atccttcgcc tccacggtat ccatgctgct cgacgtatac ctgatgagca acttcggcgc
    18421 gcggctgccc attctctcgg cggcgaccgt catcgccgcg gcgctcgtga gcggcaacgc
    18481 cattctcttc agctcccgca gccagatcaa gggcctcacg acctccacgg gcctttggtc
    18541 gtgcagcatt ctcggctttg cgatcggcgc ggggctgtac acggtcacgg ggatcgtgtt
    18601 tttctttttc ctgtgcattc tcgcgtgctt tccggcgttt gagcagtatt tgaaaaaccg
    18661 ctccaatcat tttgagatcc atctggagct caaaaacagc ggctatctgc gcgacttcgt
    18721 caccgtgagc cggcggctgg gccttcgcat cgacgatatt gaggcgaatc aggcgtatgc
    18781 cggttcgggg ctgagcgtat tcaccgtgac gttcaccata tgcagcgccg agctgaaaaa
    18841 gtataagacc ccccgcgaga tcatcgaagc gctctcgtcg ctggattaca tttaccacat
    18901 cgaggaaatg cggtaaggca gaatataagg caaaaacgcc gccggagggt cagccctccg
    18961 gcggcataat attcggttat ccggtttttt tcgctcttct tattcctcgg cgggaacgag
    19021 gaagtcctcc ttcgtgatgg tcatgagatc gtccttcgtc ggcgcttcca tggcggcaag
    19081 acggttggcc tggcgggaga tgatgtcctc aaagcggttg cgcacgtcgc ggccgttgcc
    19141 gaagttgtcg tcgcggttct tatacatatc ctcgaagaac tccttcgcgt acgtctccgc
    19201 ctcctcggtg agacggtagc cgttcttttt gcagcgcatc cggaacatgg ccataagctc
    19261 ttcgccgttg tagtcgggga aatacagata tttattgaag cgcgactgca agccggggtt
    19321 ggagttgatg aatttctcca tcggctcgtc gtacccggca acgatcacaa caagatcatc
    19381 gcggtggtcc tccatcgcct tgagcagcgt ttcgatcgcc tcgcggccaa agtcgttctc
    19441 gccgccggag gcaagggagt acgcctcgtc gatgaacagc acgccgccga gcgccgattg
    19501 gatgacctcc tgcgttttga gcgccgtctg cccgacgtag cccgcgacaa gtcccgagcg
    19561 atccacctcg acaagctgcc ccttgctcaa tgcgccgatg gcggcgtaca gccccgccat
    19621 gagccgcgcc acggtcgttt tgccggtgcc ggggttgccg aggaacacca tgtgcaggct
    19681 catggcgctg ttcggcaggc cgttttcctc gcgcagcttg cgcactttga tgagattcgt
    19741 gagatttttg acctcgcgct tgaccgtgcc gagaccgaca agctcctcga gctctgccat
    19801 cagctcctca aggctcggct ttgggggctc ttctgccttc tgcgcggacg cttcggcgtc
    19861 cgctttcttt tctccgacgg cggacacgcc cgtctgcgcc gtgaacggct gctcgccgct
    19921 cgtcacatag tccgcggcgt tgatgggtgc tttggacttc ataacgtttg ccccgtcgca
    19981 gatggcggtg agcttatcgg agcactcggt gatgtattcg gcctcggcca tcgtgacctc
    20041 gtcgtccacg gcggcgagat agagcaggat gttcgtgagc atccgcacaa aaatgcgcga
    20101 attggccgtg ccgcggcgcg cgtcctgctc cgtcagattc cagaagaaga gcggcggcag
    20161 gaaatcggcg cttttgcaca ccttctcggt gagctcccag agaaggtacg cgccgggctt
    20221 gcggcccttg gaatagagct ggttcaccgc gtccacatgc tgctggccga gccgggggct
    20281 gcccttccag agaccgagcg cgcaggacac cacaaactca tccagctcct gcgcaagacc
    20341 gcgccccgcg actttataaa acgcctccgt gttggcgata taggttttat gcagctcctc
    20401 cggggagcgt ttccagggtg tcggcatggc gttgtctcct ttttctgccg cccggacgga
    20461 cggaaattac gctctcatta tacaacaatt tttctatttg tgaacggttt tttaaatctt
    20521 tgcagaacgc ttgacattta tatataggag tggtaaatta gcactcgtag gaagagagtg
    20581 ccaacactca aaggcgccca gtgctaatgt gaggaagccg ccgtggagat cagccagaga
    20641 aaaaagaaaa tactcgcggc tgttgtggat gaatacatcc gcacggcgga gccggtcggc
    20701 tccaagacga tcgcgcagat cgccgggctc gggtgcagca gcgccacgat ccgcaatgag
    20761 ctctccgagc tcacggcgct cggctatctg gagcagccgc acacgagcgc ggggcgcatc
    20821 ccctcgccgc agggctaccg gttctatgta aacgagctca tggagcggca gagagtctcc
    20881 gttgaggagg ccgaggcgat caacgcccgg ctgagcgaaa agatggagca gctcgacaag
    20941 ctgatgagcg acgccggaaa gctcgccggt cagctcaccg gctacccggc gctgacgctc
    21001 agtgcgccgc gcgccgcgac gatccggcgg ttcgatcttc tctacattga cgcgaacacc
    21061 ttcataatcg ttgttcttct cggagacaat accgttaaga acaagctcgt gcatctgccg
    21121 ttttcggtgg agcaggggca gatccagaag ctctcggcgc tcttcaacgc gggattcaca
    21181 aacatccccg aggagagcgt gacgccggtg ctcatctccg ccacggagcg cgccgcgcgc
    21241 gacacaatgg ggctcacctc catcatcgcc gcctacgtca ttgaggtgct cagcgagagc
    21301 cgcgcgggcg cggcgttcgt caccggcgag agcagccttc tgcagcagcc ggaatttccc
    21361 gatcccgacc aggcgcaccg cgtcatcagc tacctctccg attccgacca tctcatgcgt
    21421 ctgccgggcg cggcggacag caaccccgtc cgggtcatta tcgggccgga gaatgttgcc
    21481 gaggagctgc gcgattccag cgtagtcatg gcaagctacg acgccggcga gggccagcgc
    21541 ggactgatcg gcgtggtcgg gccgctgcgg atggactaca gcgccgtagc cgccaagctc
    21601 aaatacattg ccgacggact cagccgtctg ctcggcgcag gcgaagcccc cgcgggcttc
    21661 ggaaaaatga tagttaaagg agacacattg accgatgagc agcaagaata aggaaaccgc
    21721 tccgaaggaa gagcagccca tcgaagaaac gccggtcgaa gaagcggcgg agccgatggt
    21781 cgaagaggag accgaaacgg acgcgcttcg cgccgagctc gaaagcgcgc agaacgacct
    21841 cgccgcggaa aaggacaaaa acctccggct ccgcgccgaa tacgacaact tccgcaagcg
    21901 ctcagccaga gagcgcgaca acatctacgc cgatgtcaaa gccgacacgc tcaaaaagct
    21961 gctgccgatc tttgataatc tcgaacgcgc tctccggcag gagaccgcgg acgaggctta
    22021 caaaaagggc gtggaaatga cgatgaccca gttcctcgaa gcgctcaagg cgctcggcgt
    22081 cacgccgatc gagaccgtcg gtcagaaatt cgaccccaac gagcacaacg ccgtcatgca
    22141 catggaagac cccgataagg gcgagggcga gattgtgcag gagttccaga aaggcttcaa
    22201 aatgggcgat cgggtgatcc gcttcagcat ggtgcaggtc gccaactgat ttcccgtttg
    22261 aatatatcat cccatagtta ataaaaatat ttcgtctata caggaggcaa tcattatgtc
    22321 taaaatcatc ggtatcgatc ttggcactac caatagctgc gtcgccgtta tggaaggcgg
    22381 cgaaccgaaa gtcatcccca acgccgaggg cgcgcgcacc acgccgtccg tcgtagcgtt
    22441 ctccaagacc ggcgagcgta tggtcggcca ggttgcaaag cgccaggcca tcaccaaccc
    22501 cgaccgcacc atttcctcca tcaagcgtga aatgggcacc aactaccgcg tcaacatcga
    22561 cggcaagagc tacactcccc ccgagatcag cgccatggtg ctgcagaagc tcaagaccga
    22621 cgcggaagcg tatctcggcg agaccgtgac cgaagcggtc atcaccgtcc cggcgtactt
    22681 ctccgacagc cagcgtcagg ccaccaagga cgccggcaag atcgcgggtc tcgatgtcaa
    22741 gcgtatcatc aacgagccga ccgcggctgc tctggcctac ggtctcgata aggaatccga
    22801 gcagaagatc atggtctacg acctcggcgg cggcacgttc gatgtgtccg tgctcgagat
    22861 cggcgacggc gtcatcgaag ttctggccac cgcgggtgac acccgtctcg gcggcgacga
    22921 ctttgataag tgcgtgaccg attacctcgt ggaagagttc aagaaggccg agggcgttga
    22981 cctctccggc gataaggtcg ccatgcagcg tctgcgcgag gccgccgaaa aagcgaagtg
    23041 cgatctgtcc ggcgtgacga gcaccaacgt caacctgccg tatatcaccg ccgacgcgaa
    23101 tggcccgaag catctcgacg tgacgctgac ccgcgcgaag ttcaacgagc tgacccatca
    23161 tctcgttgag cgcacgatgg ccccggtccg tcaggcgctg agcgacgccg gcctctccgt
    23221 gaacgatctg agcaaggtgc ttctcgtcgg cggctccacc cgtatccccg ccgtgcagga
    23281 agccgttaag agcttcaccg gcaaagaccc cttcaagggc atcaaccccg atgagtgcgt
    23341 ggccgtgggc gctgccattc agggcggcgt gctcggcggc gacgtcgaag gcatcctgct
    23401 gctcgacgtg accccgctgt ctctcggcat cgagacgctc ggcggcgtct gcaccaagct
    23461 catcgaccgc aacaccacca tcccgacccg caagagccag gtgttctcca ccgccgcgga
    23521 caaccagacc tccgttgagg tcaatgtcct gcagggcgag cgcgagatgg cgcagtacaa
    23581 caagagcctc ggccgcttcc atctggacgg catcgccccg gcgcgccgcg gcgtgccgca
    23641 gatcgaggtc accttcgata tcgacgccaa cggcatcgtg aacgtctccg cgaaggatct
    23701 cggcaccggc aaggagcagc atatcaccat caccgcttct tccaacctct ccaaggacga
    23761 catcgacaag gccgtcaagg aagccgagca gttcgccgcc gaggataaga agcgcaagga
    23821 agaggttgat gtccgcaacg cgggcgacca gatggtctac cagaccgaga agaccctctc
    23881 cgaaatgggc gacaagctcg acgccgccga caagggcgag gtcgagcaga agctcgcgag
    23941 cctgaagact gccctctccg gcaccgacac cgaggccatc aagaaggcga ccgaagagct
    24001 gacgcaggcc ttctacaaag tcagcgagaa gatgtaccag caggccaacc cgcagggcgc
    24061 gcagggcaat ccgggcgcgg gctacaccga tcccggtgcg cagcagggcc agcagcaggg
    24121 cggccagtac tacgacgccg actacgaagt cgtggacgac gacaaggaca agaagtaaga
    24181 tcatcccaat ttgagtccca atcaacggaa tgcggggcgg acggatttcc ggtccgcccg
    24241 cgttccatgt taagaggata gctatatggc cgataaacgc gattattatg aggtacttgg
    24301 catcgacaag agcgccagtg ccgacgacat aaagaaagcc taccggaaaa aggcgaagga
    24361 attccacccc gacctgcacc ccggcgacaa ggagtgcgag cagaagttca aagaggtcaa
    24421 cgaggcctac gaggtgctct ccgacgccga taagcgcgcg aagtacgacc agtacggctt
    24481 cgccgcgttc gatccctcca gcttccagtc gagcgccgag ggcttcggcg gctttgccga
    24541 cattctgaac gatctcttcg gcggcggctt tggcggaggg ttcggcggcg gcttcggcgg
    24601 cttcggcggc gcgcagcgtt cgccgaacgc ccccatgcgc ggcgagaacg tccgcaccga
    24661 ggtacaggtc agctttgaag aggcctgctt cggcgtcagc cgcgagctgg acgttatgaa
    24721 ggtcgaaacc tgcgacaaat gcaagggcac cggctgcgcc gagggtacga cccccgagat
    24781 ctgccccgac tgcaagggcc gcggcaacgt gacgcgcacg gcgcgcacgc ccttcggcat
    24841 gatgcagacg caggagccct gctcccgctg cggcggcacg ggcaagatca tccatcagcc
    24901 gtgcccgacc tgcaagggca agggcaaggt gcgccgctcg cgccgcatca aggtggacat
    24961 ccccgccggt atcgacgacg ggcagaccat ctccctgcgc gggctcggca acgccgggcg
    25021 caacggcggt ccggcgggcg acctgctcgt aacggtgagc gtgcgcagcc atccgcagtt
    25081 tgagcgcgag ggcacgtcca ttctctatga gctgcccgtg tccatcgtgc aggcgtcgct
    25141 cggcgcggag gtcgaggtgc cgacgatcga cggcaaggtg aaatacaccg tgcccgaagg
    25201 cacgcagtcc ggaacggtgt tccgtctgcg cggcaagggt gtgccgtatc tccacggcgg
    25261 cggccgcggc gaccagttcg tcacggtgcg cgtcgtcaca ccgaagaatc tgacggtcga
    25321 gcagaaggat ctcctccgcg cgggtttccc gcgcggcgga tatttctttt cccccggcgg
    25381 cgcttgcccc ggtgcgattt cgatggtata atgcgtgtgg cggggcgttt ggtgcgtccc
    25441 gcgttacaaa tacaaaaagt gaggcagagt gaatgaaata cgatttcacc tccatcatgg
    25501 accgccgcgg ccgcgatgct ctggcgatcg acagcgtcgg ccacggccac gcaccgggtg
    25561 cgccccgcga ggggttcgac gtcatcccca tgtggatcgc ggatatgaac ttccccacca
    25621 tcccggccgt tccggcggcg atcatcgagc gcgccaagca cccggtctat gggtactttt
    25681 tcccctcgga tgcatacttc aacgctatct tccgctggca ggagcagcgc cacggcgtga
    25741 ccggcttaac acgcgagaac atcggctacg aaaacggtgt gctcggcggc gtcgtcagcg
    25801 cgctgaacgt gttctgctct aagggcggca acgttcttct ccactccccc acctataccg
    25861 gctttaccgg cgcgctgggc aacaacggct acaacatgat cctctcgccg ctcgtnnnnn
    25921 nnnnncgaaa ccggcgtctg gcgcatggat tttgaggaca tggagcgccg gatcgtggaa
    25981 aaccacatac atgccgccgt attctgctcg ccgcataacc cgacgggccg cgtgtgggag
    26041 cgctgggagg tcgagcgctt cacggagctg tgcgcaaagc acgatgtgtt catcgtctcc
    26101 gacgagatct ggtcggacat cgtccggccc ggcgttgtcc acacgccgac gcagtccgtg
    26161 agcgaggacg cgaagaaccg cacgctcgcg ctctacgcgc cgagcaagac gttcaatctg
    26221 gcggggcttg tcggctcgta ccacatcgcc tacagcccgt ggatacgcga ccgcatggaa
    26281 aaagaatgtt cgctttcgca ctatgacagc atgaacgtgc tctccatgca cgcgctcatc
    26341 gccgcctatt ccccggaggg cagcgagtgg gcggacgagc tgtgcgccgt gctcgcgggc
    26401 aacatcgatt tcgcctgcaa ctatatcaaa acgcggttcg acggcgtgga ggtcacgaag
    26461 ccggagggca catacatact ctttgcggac tgtacaaaat ggtgcgaagc gcacggaaaa
    26521 accatcgacg acgtggaaag agcgtgctgg gatgtcggcg tcgcggtgca ggacgggcgg
    26581 cagttccacg gcccctgcca catccgcatg aatctcgcgc tccctctctc ccgcgtgcag
    26641 gaggccttcg accggctgga caaatacgtt ttcaacgcat aaaaccatat gaaaagagcc
    26701 gatggcgttt tgccatcggc tcttttacag gaaggcctcg cagagcttgc gcccgcaggc
    26761 gcaaagaaaa ttcaaaacat ttttgtcggc ggcgtgtacg tcggcaaaaa tcagccatcg
    26821 acgggcagga aacccgtgcg tcggacagcc agtccttt
//
LOCUS       DAWAAE010000010        26824 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_786_length_26824_cov_3.355112, whole genome shotgun sequence.
ACCESSION   DAWAAE010000010 DAWAAE010000000
VERSION     DAWAAE010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 26824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26824
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_26824_cov_3.355112"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    19419..19428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21720..21729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22589..22598
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23952..23961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4717 a         7923 c         8244 g         5900 t
ORIGIN      
        1 ctgtcttgcg tcgccccagc ccgcgccgaa gtcggcatac gcggtgtaaa gatccttcag
       61 cgggttgtcc tgcggcgact gcatcaccgt cgggcgcgtg ttatagacga tgttcacaag
      121 ctccttcatc aatcggcggt cgcacaccgc ccactgcttg ctcgtaaagc cgtccgcacc
      181 gccgcccatg acgatgtagc gcatgccgta cacggggttc gcggcgttgt aggcgctctc
      241 gggatcggcg gcaggcatca ggcgggcgtt ctcgccgaac atcttcttcg ccttttcttc
      301 aagctccggc gcaatgagca ccgtgtcaaa gtcacacagg aacggcatac cgtcgggcgt
      361 gagaaatcgg ttggcacgag cctgcgccgc cgtgatggcg gaaacggaga aggcgtccgt
      421 ggagatgttc gagtatgtac cggcgtcggt atcggccaca aacgttcggc ccgaggagcc
      481 gcgcgaggcg acagggtgcg cggcgttggc ccagctcacg ccgtcgccgc cgttgtgctt
      541 tccgtcggtg ttccatgcgt tggcgaacat gcgcagaacg tgcagataca cggtgagcgc
      601 catgctgtcg ccgagcttcg tgccgacctt cttggtctcg cccatcttgt ccaccttggc
      661 ctccttgtag ccaacgggga tggagagcgt gtactcgacc ggcgtgatca cggtcttaaa
      721 gccgcgcttg agactgccct cgttgagatt ctcgccgtcg tagcggggcg cttcgccgta
      781 gccgccggag ccggtcagct catagtcgat gctcttggcg ttcacctcgc ccacgacggg
      841 cgagagcttg ttgaggcggt cggcatacgc aaagtcgaac gccttgccga cgaacgcata
      901 gttgtcgctg gtccagtttt tgaaagtgct gctcattgat tcattttctc cttttctgtt
      961 tttgttttta agattttgcc gcgagcgtat gcgccgcggc gatacagcgg atcgtgtgac
     1021 gctcgtaatc gtggccgatg cagcgcaccg ccgtggcgcc ggtcgcgctc acaacgagcg
     1081 ccgtagcctt ggcgttgagc gcgcacacgg cgctgccgag cgcggggtag aactcatact
     1141 cgtccccggc ggcgggtgtg ccgccggtct caagcgtgag caccgtgccg ctcttcgcat
     1201 agtccgtcac ggtgcggcgc gtgccgggct tatcgctgtt gctgctgccc gcggccttgc
     1261 ttttgagtac gagcaccgcg gcgttgtagg cgtcgtccgc cgcgccggca gcaatatccc
     1321 cgcttgccgg aacgagcgtc gcggcgctgc cgctcgcggc cctgatcgtt ggcacgggac
     1381 attcaaagat cagccccgga ttatcgcaga cgaggatctc gtccccgtcc gcgcgcggat
     1441 tgagaatgtc ctccgtgccg ctgtggttct ctccggcgat gccgagaatt gcagcggtct
     1501 gcgcggcagc cgccggaacg actttgcctc cggaaagctg caccacctgt ccggcggtga
     1561 tcgccgtggc ttttgccacc gggtagctgc gctcacggat cgtctcttcg ccgcccgcat
     1621 tctggatggg tctcatgttt tgtctccttt cctgttcatt tgtttcggct cagatactcg
     1681 cgagccgaca ttttcatctg cggaaacgcg cggttccacg cgtccagctc cgactgctgc
     1741 ctggcgttga gcgcctcata ggccccgctg ccgccgctgc cggtggagcg tgcagcccgg
     1801 ctttcccttc tcgcctgcgc agcccgcagc gcgccgccgg caacctccag ataatccgcg
     1861 tacagctcgc tcagcggctc tctgccgtag cggctgccgc agaaacggcg aaagctctcc
     1921 gaagcgtcca gccccgcgag atccgcctcc ggataccggc gggcaaactc gcgcgcgtcc
     1981 tcggcgataa agcgcatctg ctcttcctcg cgcgagcgcc gctcgctctc gcacccggcc
     2041 agctcacgca tcgcccgctc gtatccggcg cgttcgcccc ccagtctcgc cgcctggaaa
     2101 cggcgattgt cctcatggct ctgcggcgcg ggaacacctt cctgctgctc cgccgagccg
     2161 tcctccgcgg gagtcacgac ctccccgacc tgttccggtc tttcgttttc gtccatctca
     2221 gactcctttc cgccggtgtt cgccccggcc cgcgtattcc cttacggtga tttaatggta
     2281 gcatggaaac acagttcgtg aacggcagct ttttcgtcgt ttttcacgaa tcgtgtatcc
     2341 gctgaatatg cagggtcttt ctccggcgca aattgctcat ttgccaattc ccgcgcccga
     2401 gctgcacacg ttccgtgata atgccaaaca caaaaacgcc gccggaggaa atcctccggc
     2461 ggcgcagcgc ggtgatttgg ttttacagat tctttgcccg tatttcatcg aaccacatgc
     2521 ggcagcgggc ttcgtcagca tattcattca agggcttcaa gcgctcatag ctgtactgcg
     2581 ctcttacttg ccgcgtcttg gcatcataca cgatcttgat caccgtcggc atcgcccttt
     2641 tgtattccag acacagctcg cggactgcgt aagcatagcc aaggattgtc caacgtatat
     2701 cgctcttgtc atttctggaa agagaagagg actcttttcc ttcacgtatg gctttatccc
     2761 attcgtaatc cggatagaac ttcccatcaa tccggaaagc gggccttgcc atcatcgctc
     2821 cgtcctcaag gtcggaataa atataaacct tttccgcacg gttttctgaa agggcaatgc
     2881 tctgccggat catttcactc tggtaggcag aaaacagatc ctcaaacgga cgcccttctg
     2941 ccgcagcttt tttgttgcga gctttcaggt ttttttccaa ttctccaatg ttcataatcc
     3001 cattcctccc caaagatcat tcgtgatacg ccgactatac tctactccat caataaaata
     3061 ttttatgtaa aatacactcg gtcgcaaatc gtctccctcg tactcgacac ggacatctac
     3121 ctccaccgct tttccagcct gtatcgccct gtgccatttg ttctcaagca ccttataaga
     3181 gctcaggttt acaagccaat actgcgacac aaggttgtcc aatttcccgg agccgccgaa
     3241 acggtcgccc gccagatgcc tggcatgatc gtccggctgc tttcccggcg tgtttcgtac
     3301 atacggcaga cgcccggtaa tggttcgtcc tttttctcat aatcatactg taccatgctt
     3361 tgctgaaaaa aacacggact tttccagtac gcaaaacgcc gccggggtgt cccccggcgg
     3421 cgcatgcatt attcaattaa tccgtctctt cgtgcctttt caaatgcttc gtgtacttcg
     3481 gcgaaaaaga cctttatttt tcgtgacatc tcctccggct tgtccagcag gaaatacgca
     3541 tcaattccgg tatggatcac cgtattcata tccgggtttt ccttttggcc ggaatgaaac
     3601 accaaaagat ttgcaatctt tacagacatc cgtaccgaat aatgcaactt ctgataggcg
     3661 atggccgtaa tcgttccatt aaaccagccc agtctcgcaa agccattctc atatgcaagc
     3721 gcacatatct ccccgaaaat cagtccggcc atcgttgcgc ggtctatatc gtcgtattcc
     3781 tccaccggtt tcccgtaggc cgcgatcgtt tgatccgcta ttgcaaaagc cttcggcgag
     3841 agctcgttca tttttgcctc cacttccggc gggagctgct gttctgcttc tttctgccga
     3901 tccggcggca ctctcttttc cttatccatt tccattctct gaatcctcca atcatttcag
     3961 atgcattgag ccgggtagtc tcggaatcag ctcatccatg gagaagccat aatcgaactg
     4021 ttcctgaacg gcggcattta taaaggcttc cacaagatcg tttccaaacc ccgcatttcc
     4081 tattgatttt gcgttttcaa tgttatactc gccgattgtt ggaagttctc cttcgtattc
     4141 accgcctgct ctgtagttat taaaaaaacc ttccgtccat atgtgcagca gattgtgttc
     4201 actgcccttt tcggtgattg catttcctct aagcattatg catacaccgt cccgataggg
     4261 aattgtttct ccgaaaactc cctgctggtt caaatggtgc agctgtacgc cggtaatacc
     4321 ttccagcttt cggaacttgt tcaaatcgcc gtagcttcca tagaggacat attttcctgt
     4381 tgacggatca attatatcat ccgacatcaa agctgtcatc actttttgat cgaacgccgg
     4441 atcaattcca agtcggcgtg catttccgcc tccggccccg gcattctgtg ccgcacgtag
     4501 tacaacgtcg ttataccagt cgtttgtggg catctccggt gctctggctc cgccgtcacc
     4561 attcagcccc tgaataacac gctgatactc gtccgacccg agcttttcct cgactttctc
     4621 ctgtgcctga tcgagtaaaa ctgcgtaaat tgggtctgta ggctttctcg atttttcagc
     4681 aagtactcct ccgctttggt agagcaggct ttctccaaag ccgataagat agtcttcggt
     4741 aagctgcgct acatccacat cggagaaata ttcctgtgcg ctgctgtatt gttttccctt
     4801 atagatcgtt gtaaaaagcg ggtctaaaac atcgtcaata acgttttcca gcccttcgtt
     4861 atttgcattc aacaggaaac gcaaaactat ctttccggca gggctttttg caagcttatc
     4921 caccacctga ttcgttaatt tgtcggaaaa tcccttgccg aaaagcttgt tcattccgtc
     4981 gctgaggcgt tctgtgacga cagctttcat tcctatgccg cttgcataca gctctgcctc
     5041 gtgccgcgtt gccccctcgc tcagggaacg gtcaaaggct gcgccggatg actgcccgta
     5101 cagcattgca ttggaaagga atggtccgaa gattggaata tattttgccg ctatagccgg
     5161 aactctgctg cccaggcgct ccgcaaattt tcccgcaaga cgcaaaccgt ccgagacatc
     5221 tccatacttt ctctgtgttt cggcgttata ctcatccgcc cagtacgcag cgcggtacag
     5281 cggctcatca gcgtatttcg cctctgcttc ccgctgcgcc tgaagaaggt actccgaact
     5341 cgcgtccagg ctacccatat attcaagcgc agtacgctga ttttctgtca atccggcgta
     5401 acgctcttct cttgcggctc tggaagccgc tccaccgttg tatacatcct cccaatatgc
     5461 ctgttctgct gcttggtatg gcattgcaag ctgtgagcga taactaatat catttgccag
     5521 cgcttgcgtt gctcctttcg cgcccccgac agctctccgc cacaaatagt ccgcgcctgt
     5581 acctttcgct tccaatgcgg cacgatagtc ggctgcattg tctacgttat aacgcgccca
     5641 ctccgcttga accatgtcgt tgaccttgcc ggtgtagccg ccgcgcaggc cgaggatttc
     5701 cgcctcgtcc tccgcgctgc cgtacagctc gcgatactgc cgcgcccgtt cctcctgcgc
     5761 tcttcgcttc gcttcctgct cggcaaagaa ctttttctgg tactctgtgg tgctgcttac
     5821 gcccttctgc cgcaggaaat cgtcggcgac atcctgcgcc tcgccggtgt aggagttcac
     5881 acccatgatc tcgttcatgt cggcgagatc cgcagcgctg gcatacgaag aacggtaagg
     5941 cggtttcccg gtcgttcccg ttgacttact cgttccggtt tgtctggccg tacccgttgt
     6001 cccggttctt cttatcgttc cggtcgtgcc gggcgtctgc ttccctgcgc cggaattgcg
     6061 cagccacggc tgcgcaccgg tgctcgccgg ccggccccgc gtcggcggct tatagttctg
     6121 ccgggcgttc tgccctcttt ccggaacgat tccccgctgc gccggtgttg tgttttgcag
     6181 cgtcggcgct ctgctctgct ggctctgcgg gatgccgacg ccccggttcc gctcctgcgg
     6241 cgatttcgcc ggcgttatca ggttttgttt ggtgttggac ggatagtccc acaccggata
     6301 ggatcgttta ctcatatccg ctcctctgct tacatagatt tgaatcacgc tttgtgtttc
     6361 atatctatat gttagcagaa aaacacattt tgtgagcacc tactttcccg tcggatttca
     6421 cgaatcgtat atccattgat aatgcatagg ttttcgactg caaaattttc tggccgacaa
     6481 tttgagtccc aattatacgc acatttcgtg atattgatgg gcatagaaaa actgccggga
     6541 tcatacgatc ccggcagctt tgcggatatg aaattatcgg ggagaattac tcgacaacaa
     6601 cgatgccgag cttcttcgcg gcttcctgcg ccttctcgag agagggcgtg gcgttgcgca
     6661 gcacgagacg gccgccgccg cggttgagcg tatcgaccat aacgctgttg cccttatcgg
     6721 tgacgagagt gaccttctgg atctggtact cttcaccgcg gacgatgttc accttgacgc
     6781 cgtcggcctt gacgcgctcc acggcgacct cgtaggtctt atagtcggag gtggagcagc
     6841 cgtacaccgc gcccatgagc acgccgggca cgttgatcgt gcggcgggcg aagagctccg
     6901 gatacagttc gatggtgatc tcggtaacct tctcgccctc attgagaagc tcgtgggcga
     6961 tgcggccgca gtttgtcggg ctgccgacag cctgagagga accgccgaca acggcgtcgc
     7021 cgagcgtctt gatgatgggg gcagagccgt ccgccatctt cttggcgatc tccttggcct
     7081 tttcgagcgt ctcttcgatg cgcttcttgt cggccttgtg gacttcctcg gaaacgagag
     7141 attcgacctc agcggtgcgc tggaagaagg gctccatgta ctccacgacg gtgggtacga
     7201 tgtggtgcgc gctctccttg tgaacgagag acgccatagc aaacatgacg tcgaacggga
     7261 cgttgacggt catctccacg gtcgtgcaga gacggccggc gtacatgccg ccgaggatcg
     7321 cgccgccgac gtgggcggtg cagagcgccg gaacgagcac gcgcggcgtg cagggaacgc
     7381 cgatcgtcgg ggacatggcg aggaccatgg ccttttccat ctgctcgggc gtaccgttct
     7441 tcagatgaac ggtcgcggcg gcgatggcgg cagagcccgc gccgtagcct tccagattgc
     7501 agccggtggt gaccttcgcc acgcggaaca ggctgccgac cttgagcatg aggcaggcga
     7561 cctcggcgag agtggtctcg tcggtgccga catccatcag cgcgcgcagg aagccggtgt
     7621 aggggcaggg gtcgcccgtg ccggcgcacg gacgcacgcc gatgcagtgg ttgccgactt
     7681 cggcggccac ggtgtacatg agcgccttgt caagcagcac atccgcaaag atggtgccgg
     7741 ggcccatttc cttgagctgg gtaggaacgg tgccgagcag gaagctgttt ccgccggaaa
     7801 cgccgatctc cacagccttg aggttgtgcg catactcttc catgacggcc ttgagcacgc
     7861 cttcgcggtc aagaccggtg cgcagctcgc tctccgtgag gacaacgtcc accacgcgca
     7921 cgccgtattc ttttgcgagg gcgatcgtct cgcccatcgt cagcggaagc ttttccgcaa
     7981 ctacgctttt cagatctgcc atctttttct ctcccgtctg cgctcagcgg ccggtggagg
     8041 aggcgtaggt gtgctcgatg tcgcggatct tctgcagacc catcagctcg ttgaactggg
     8101 caaacggaac catcttgtca agctgggtga tggtggtgcc gtcgcgcttc aggccttccc
     8161 acaggtcgat catggccttg gtggtgacgt aggtggaggc ggtggggtag ataacgaggt
     8221 tgtagccgaa gctctcgagc tcgtcgttct tgaagatcgg ggtgcggccg ccctcgacca
     8281 tgttggcgag cgtcagaccg gggatctcct tgttgatgag catcatctct tccttggact
     8341 cgggggactc gatgaagatg atgtccgcgc cggcttcctt gtaagcaagg ccgcgcttga
     8401 tggcctcttc gatgccgtaa acggtacggg cgtcggtacg ggccatgatc atcatgtcgc
     8461 cctggcgggc atcaacggcg gccttgacct tgccgaccat ctcttccatg ggaacgacct
     8521 gacggccggt catgtggccg cacttcttgg gcatgacctg gtcctcgagc tgaatgacgg
     8581 cgacgccggc cttctcatac tcgcggatgg tgcgctgcac gttgacagcg ttgccgaagc
     8641 cggtgtcggc gtcggcgacg acgagggcgt tgccggcagc ttccacggtg ttggaagcgc
     8701 gcatgaccat ttcgctcatg gtcatcaggc cgacgtcggg ctggccgagg tgggaggcgc
     8761 tctggccgta gccggtcatg tacacggcgt cgaagccgag gtaagaaatg atcttcgcgg
     8821 tgagagcgtc gtggcagccg ggggcgacga cgagcttttt ctcttcaaag agcttgcgca
     8881 ttttctgtcc gttggtcatg ggaatcagtc tccttaataa gtataagtat ttaagtggtt
     8941 tatttcttta tcacatgctc tttcatgtac gaaagaactt cttcggggaa gtgctgcgcc
     9001 agaacgccca tggcgtagag gatatagtcc tcgtcgaacc agatgtccgg gtcgttgagc
     9061 ggcagcaggg agatgccggg gttatcgaac gtgcggcgca cgatgttctc gcgctcctcg
     9121 ccgtggcagt ggaggaaaat accgcccacg cacagcacct ggcgcacgtt gcgaagatcg
     9181 cgccccatcg gagcgccggc catgatgccc atgacgggat cggcctcctt cgcgtaatac
     9241 ccggcgtgac ggcggatggc gacgtccgcg gcgcacgcgg caagcgcgcg gtcgagacag
     9301 gcctccttct cgtcggcggg aatatgatcg ttgtgcgctt cgagcagatc gacatagttt
     9361 tccacatcct ccggcgtcag accgtaatcg tggtccatgg cgcggagaac ggccttgtcg
     9421 cccacggcct cgggaatgcc ggtcgcgctc acgcgcagac cgagattgcc ctcgacggtg
     9481 cggtaggaaa actgcttttc gttgctcaca acaagtccgc gctcttcgat gttcatgtcg
     9541 atgagctcgg gcagggcgga atggatgtcg gtcgtagcgc cgccgatatc cacgaggatc
     9601 acgccgccga cgccctcctg ctcgtagctg cccttggcaa gcagctcgct cgcaaggagc
     9661 acggcgcccg gcgtcgggat gacggtctga tccgcgagcg aatcgcggaa ctcctgcaag
     9721 cccttcgcat gggtgatctg gttgatgaac tgctcgtgga tggcgatgcg cgcgggcttc
     9781 acgttcagct cgtggatcgt cggcaggatg ttcggcacgc ggatgcagtc aatgcccgcg
     9841 gcgcggaatt tctccgccgc ctcgcgctgg gcgaacttgt tgcagccgag aatgaccgtg
     9901 ccttcgccgt gcaccttgca gatgatgtcc gcgttttcga gcacgttggc catgtcgccg
     9961 ccgtccgtac cgccggagag caggatgatg tcgggcttga tctcgcgcag cacttcctcg
    10021 cggaactcgg gcggctcgtc cgcggagacg acctccatga cacgcgcacc ggcggtcatg
    10081 gcaacttcct tcgcggcctt cgcggtcacg cggggcatgt accccatggc gaccatgcgc
    10141 agtccgcccg cggcgctgca cgagctgaac cgaaccatat cgggcgcgaa ttccacgccc
    10201 ttggcgcgga tctccgcctc ggcgccgcgc gcgccgtcgc tgatgttctc aagcgtcgtc
    10261 gggtgctggc cgcgggcaag gaacgtcagt ttcccgtccg caagggcaaa ggccgcgccc
    10321 ttcgtatagg tgctgccgac atcataaaca agaatgtttg ctttcatcgc ttcgactcct
    10381 taaccgccat ccgggagacc cggatggcgg acatgggtgc tcttacttct tgcgggggtc
    10441 cttgctcttc tcgagcaggg cgtagccctc cataagacgg cgggcggtac gctcgatggc
    10501 ggccttcttc acggtgagat cgggcgtgac ctcggagtag cagttgatga cgccggcggg
    10561 gtggccgatg cgcacgagct cttccgggtt ctccttgccg atgacctggt tgacgatggt
    10621 gccggggatc ttcgcggcga cgccggtgca gaccgtggcg gtgccggcat aggtcttatg
    10681 cagcacctgc atgaacatca ggcgggagac gagatcgacc tcttccttct tgatggtctt
    10741 cttctcgggg ggatagtagt aatcctgcgc ctcggtgatg aaagcgacca tcgggaacgc
    10801 cggggaattc ttggtggcgt cggcgagatc cttcgccatg ccgcacttga cgcacgcgat
    10861 gccgcggatc tcttcgagat acttgagcag ttccttgttg ccgtcgatct cgacctgatc
    10921 ctcggtgccc ttcatgccga cgctctcggc gcgcacgaag atgacggggt tggcgcagtc
    10981 aacgatggag gcctcgatgg ggccgcggct cgtctcgatg acgtccacgg ggttgccggt
    11041 cggcaggagc ttgccggtgg cggcgccggc ggtgccggac atatccatct ggatgagagc
    11101 gccggtgccg ggcacgcccg cgatggcgac gtcgccggtg accttcgcct tgccgtcctc
    11161 gacgaggacg taggacttca tgaccttctg ggtgttgacc tgccagatgg taacctcgtt
    11221 gagaccctcg tggcacttca cgatggactc gtcgatcgca aacggagcaa cgccggagga
    11281 gatgttgccg cagttgccgt tccagtcaac gacggcgtcg gtgatggaga cctgggcgaa
    11341 cgtgtagtcc acgtcgcagc cgggctgctt gctcgggccg atgacggcga gcttgctcgt
    11401 caggggatcg gcaccggcca gaccgttgat ctggcgcttg tcgggggaac cgaagatctg
    11461 caggatcatg gcgtcgcgct cggggccggg ggcgggcaga tcgttttcct tgaggaaaat
    11521 gcccttgctg gtaccggcac gcatgataac gcagggaatt ctgtactggt ccatggtgtt
    11581 ctctttcctt cctaaattac ttgttttctt ctttctcggc gagctctttc tctttcgcct
    11641 cgatgagttc ggtgatcttg gtcacgcact gctcgggcgt ggactcgggg ccgaagaacg
    11701 cgtcgacgcc cataaagccg gtgccctctt ccgcgatctt cttctcgtag taggcgtgct
    11761 cttcttcctt ctcggcgata cgcccgccgg cccagacgac gaccttgtcg cgcattccga
    11821 gctcttcgag acgcttgccg acacgcggga acagcgtcac gccgaggccg agcaggttgc
    11881 tcacgccgat ggcgtttgcc ttcgcctccg ccgcgacttc ggccacggtc tcgggcaggt
    11941 tcatgccgcg caggaagatg atctcataac cggcgtcctg gaacgcctcg cggatggtgt
    12001 tgatgccgtt gacgtgcgcg tcggcgccga cggtcgcgag gacgatcttc tccttgcgtg
    12061 taggcggctt ctcgatgtgc acggtaacgt gctccttggt aatcgggcag cgggaaggat
    12121 cgacgcccag cggagtgtag ccttcgacgt agcgggtgat gccgtcgggg tcgcggttcg
    12181 tcttgacaaa gcgcttgaga tcccagccgc cggcgcgcgg ggtgtccatc ttgccggact
    12241 tgccgccgtc aacgatgagg tcgatgagct cttcgtccgt ccactgcttc cagaattcct
    12301 cggtcaggca gccctggtgc aggctgtgcg cggggagctt gaggacggct tcaagaacgg
    12361 cgaacgcctc gtccatcatc atgttctcgt attcgtcgat cttcggagac tcaatgccgg
    12421 ggttgtaggt gtgctggaac acgtcgagcg tagcccagca agcctggccc atggagtcgc
    12481 cggtcgggat gccgacgctc tcggccgcct tcgggcggta gaagttggcg ccgccgagct
    12541 tggcggagat ggccatgcgg gcaaaatgcg cctgctccgc gatcaggttc gcgggcggat
    12601 gctggaacgt ttcgggaaca acgatcaggc tcttggagag catgttgtgg cggaacgcgc
    12661 ggatgatggc gaggtcgcgg tacaggttgt tgccgccgac gttcatctgg atgccggaga
    12721 gctcccaggg gaggccggcg tgggtggcaa taccctcgga gagaaggcac atcgcgatgg
    12781 ccatgccgtc ggtgccgccg atgttgttga tgtgcttgtg ggtgtcgagc tgcgcgaaga
    12841 tcttgttctc ggcgcagacg atgcaggact tgtaaccgtt gacgaccttc gtgcggaagt
    12901 cgtggatgcc gcggccgccg aagtggacgt ggatgacagg gccgatgtcg gtgccgtcaa
    12961 agccggcgat aaagccgttg aggatgttga tgtgcgcggt gtcgccggtg gcgttgatac
    13021 gcacggggtg cttcgcgccg ccgcccatct ggatgaactc gcgctcgccc ttcggggtga
    13081 tgccgccctt gccgcgggac tgctcaatga gctcgcggcc cttgagcggg tcgatgtggc
    13141 gggtcgcctc ggagtggacg aactggaaca cggtctggcc gaacttttcg gcgtacttgt
    13201 aaatgtcgtt ggattccttc caggtctctt cggcgctgtt gcggccgagg atcgggttca
    13261 ggcagggcat gccggtctcg acggccttct ggccgagctc gtagatgcgg tagccgctgc
    13321 ggacgacacc ggcgacctcg cggggatacg gcgcgggcag accgacgacc tcgccgttct
    13381 cgtcaagatc gggcatctca acgccgaacg catcagcata agcgcggatg gtctcgatat
    13441 cgtccttaat aatttgtttt gttcttgcct gagggtcttt catagccatt gctctcctcc
    13501 tctgtatatt ccttatttga ttctttcaaa ccggtttatt cggtgatgcc gagctcggcg
    13561 cgtacacggg gaatcagtcc gccgtattcg agcgttttga gcacgttctc cgaaagaggg
    13621 ttggcctcga tgacgccgca gggcgcggtg atcgtgcctt tttcgagatc gacctcaatg
    13681 atctcgccgg tgtggagctt cttcacggcg tcggcaacga acaccggcat gccgatgttg
    13741 atgcagttgc ggcggaagat acgggcgaag ccctgcgcca cgacgagggc gacctgcgcc
    13801 tgcttgaggg cgatgggggc ctgctcgcgg gaggagccgc agccgaagtt gcgtccggct
    13861 atgagaatgc acccgggctc gcggttgagc ttgaagtcct ccgtcgggag gccggccatg
    13921 gcgtacttgc cgatctcctc gtagggctcg ctcatcgcgt agccggggag gatctggtcg
    13981 gtatcgacgt cgtcgccgag aatgataact ttacctctga tcttttccat ttgtgcttac
    14041 acctccggaa cggtgatgac gcccgtgagg gcggtggccg ccgcggtagc cgggctcgcc
    14101 agatagatct ggccgacctt gctgcccata cggccgggga agttgcggtt cgtcgtggaa
    14161 acgcacacgt cgcgcggggt gataagtccc tcgtgcgcgc cgaagcagga accgcagccg
    14221 gggttgagaa tggtggcgcc ggcgtcgcgg atgatcttcg tccagccgcg ctcttccatc
    14281 tgtccgagga tctcgcggga ggcgggaacg atgagcacgt tgacgtcggg gctgaccttc
    14341 ttgcctttaa atgccttggc gacctgctcc atgtcgttca gacggccgtt ggtgcagctg
    14401 ccgacgacga cctgcgtgac cttgatgccg gccttctcgc gcaccggaac aacgttcgcg
    14461 gggctgaagg ggcaggagat ctgcggttcg agcgtggaaa gatcgatctc ataggtctcg
    14521 aagcagtcgg attccttctc ttcagtgccg aagatggtga tcatgctgcc gatctcgata
    14581 cccatgttgc agacggtcat gcgctcggcg atggagagat cgtcgatgcc ggggccggta
    14641 agaagaaggc cgcggtccac gagatagtcg gagccgaact tgctgccgag atacaggatg
    14701 agatccttgc cgaaaacgtt ggaggggcgc ttgccggtga gatatatctg cacaacctgg
    14761 ggcacctcaa gatcgagcgt gccggtcgcc atggccgcgg ccagctccgt ggagccgacg
    14821 ccgatgccga tctgaccgta accgccgcag gtgccggtgt gggagtcagc aatacaaacg
    14881 atatcgccgg ggttctgctt atgcttttca tacatcagct ggtggataac gccttcaccg
    14941 cgctgataca ggggaacgcc gaattccttg gagaagtcgt ccatcgccca aacattggcg
    15001 cgggccttct ccgtggcggc ggggtataca tggtcaacga tcagcgccac gcggctggga
    15061 tcgaagacct tcttcacgcc gatctcgcgg aacatgcgga tggcgatggg agccgtcacg
    15121 tcgtgcgcgg cgacaaagct caccttgcag gtgagatcct ggccgggggc gacatgttcc
    15181 ttgccagccg cttttgccag atacttttca ataaggttca tttacaatgt cctccaggcc
    15241 ttttctcccg cagatgattc tgcggtcatg ctcttgaaaa tagccccctt gttgccaagg
    15301 gggctattgt taaacgcggg gaagaattag tgcatgccgt cctcgcactt ggcttcggac
    15361 ttctgcagtc cgccgatacg gccaacgccg ttgacgagag catgcggacg gccggcgtca
    15421 gccagaacga ccgccatgac gatctcatcg ctcttcgggg catcgggaat acggaccgcg
    15481 aaggagccgt agtgggtgcg cacgaaggca gcatctttga agtgcaggga gatgtccagc
    15541 tcggagccgg cagggccgac cttctcgttg gaaggaatga tggacttgcc gccgccgata
    15601 gcagcgcgga tggtcttgcc agacttcgga tgcagcatag cggaggagtg ctccagctcg
    15661 ccgtcgagac cgacgatgca gcccttgccg taggtttcaa cgttctcggc gccgaggagc
    15721 tcggcaccct tcttggtgag cagatcgccc aggtactcgc cgtactcggt cagctcagac
    15781 aggtcttcga cccactggcc ggcgtacgga ttcttgataa cggcagccac ggacaccgtc
    15841 ttgatggact tgcggccgga gttggtctcc acgggcttct cagcgccgcc ctcgtagaac
    15901 acttcttcga ggttggtaac gatcttgcga atcttaggct ctttcattgg tatatcctcc
    15961 tattaaaaat taatagctaa atgagttggg cttctgtgcg agcaaggctc agcagggctt
    16021 tctcgacgca ggaggagtgt tgctggactt cttgacgttg atcttgccgt caatgatgac
    16081 atagctgagg cccggaatat cgccggcggc gaaagcttcc atggcgtcct tgccgacgga
    16141 acccatcggg gtatccatga tcagcaggtc ggcttcctta cccggggcga tgacgccgcg
    16201 gttgagcttc gtgtagacct tcgccgggtt gccggtcgcc atggcgacgc agacttcggg
    16261 ggcgatgtcg gtcatggaag cggcgaagct gatgttgcgc atgatgccca gaggaatgat
    16321 gcccgtgccg gacggagcgt cgttgccgaa gatcacgcgg ccgagctggc ccttttcggc
    16381 agcgcggttg aggacgtact gagcggtctt cgggttgccg cactgcacga tctccatcgc
    16441 gaacggggtc tcgtccatga tgcggtcaac ttcgtgcaga ggaatggagg tcgggccgcc
    16501 attgatgtgg gaaacaacgt cgggcttcgt cttgatgacg tcgtccgcgg taacggtgga
    16561 ggagccgggg atggaagtac cgccggtgtg catctgcacc ttgaagccgt gcttgtgagc
    16621 ccactcgacc atgggagccg cagtctcggg gttgttgacg gtgccgaggc cgatctcacc
    16681 gacgacccag acgccttcct tggcgaggtc ttcgaagtcc ttctcgacca gacccttctc
    16741 gaggatcagt ccgccgcctt cgaccttcac gccggaaacg cccttggcaa agcacttgtg
    16801 gccgacaatg gcctgcgcct tggtgtagac ggggtcgccc ttcggatggc cggggatatg
    16861 gcattcgccg gcagagatca tagtggtcac gccgccgttt ttcgcactct caatgaagcc
    16921 aaaggtcttc tgacgggtgc cgtagccgtc cgccgtggag acatgcacat gcgtgtccca
    16981 aagacccggg gtgacggtgg tgccgttggc atcgacgacg gtcgcgtcgc cgacgttggc
    17041 gaggagttct tcgccgccga tcgcctcgat gaggttctcg ttgatgacga tgacgctgcc
    17101 ctcaagaacg gggtggtcaa tgtcaccaga aacgatctta ccgatgttct taattaccag
    17161 agtagccatg gtaaactccc ttctgctgct gcgcgcagca attaaattgt gtgtttgccc
    17221 aactcccccg gcgttgcgca gttctgcgcc gggagcgttt tgccagagta cacgcggaca
    17281 ttccgccgcc atccgcacat ctgggatatg gcagcagccc tgtccaaggg gtttttctgt
    17341 cgcggcaggg gtttgcccgg aaggacggac gcccttaccg tgcagaaaac ccctggttcc
    17401 ccggtcaaac tgtggctggg gagccgactt ttgattccct aagattctcc gctcaatccc
    17461 cggcgcacaa ggcgccggga accggcgaat attgaataac tcaacgcctt ttctaaaaaa
    17521 tccgcgcacg agctgtgctt acctcggagc tgcagcttgc cccgggagta agtaaagttt
    17581 tgtgcctgtt aggattacag atcgagagcg tagtcgcctt ccttgatgag gcccttcttg
    17641 cgcatgagct cgctgacgag ccagctgaca agagcgggga tcacgaagga gcagacgacg
    17701 aggccgatcc agttcagagc cgggctggcc gtagcggcgg cgaagtcaac gccgagagca
    17761 gcgttctctt cgcacatggg ggccgcccaa ccggtgatga ggccgatctg gccgcagagg
    17821 ccggaggtgc ccatgccgga gttgatggca gcgccgttca tctggagctt gaacaggcag
    17881 gtggcgagcg ggccgttcac gatggaagcg accacggggg gcagccagag gatgggtttc
    17941 ttgaccaggt tgggaacctg gagcatggag gtgccgaggc cctgagaaag gagaccctgc
    18001 acgccgttct cacggtagga cgcaacagcg aagccgacca tgtgagcaga gcagccagca
    18061 acgcaggcgc cgccggcgag accgaccaga ccaagaccgg cgcagatagc ggcggaggag
    18121 atggggaggg tcaggatgat gcccatgaga gcggagacga ggatgcccat ccagaagggc
    18181 tggagagcgg tcgccttcat gacgaggtcg ccgagaagga cggcgccctt gccgatcggg
    18241 ggagcgatca ggaaagcgaa cagaacgccg acaacgatcg taacgaacgg ggtcacgatg
    18301 atgtcgatcg gggtgcgctt cgagaccagc ttgccgaaga acacggcaat gacgacgacg
    18361 aagaacacgg cgagaggtcc gccctgcgcg ccgagggcgt tggcagcgaa gccaacggcc
    18421 agcgtggagt acatgacgaa cgggggagcc ttcatgctgt acgcgatggc ggcggccata
    18481 gcggcgccct gcacggcgta agcagcggtc ttggaagcga tcagcaggtt agcgcccgcg
    18541 agagcttcgg aaccccaggg gttgacgacg atgtccttca gcaggggaac gtacatgccg
    18601 atcgtgttga tgatgcagcc gatcagcaga gacgcgaaca gaccgtgagc catgccgccc
    18661 aggccgtcga tgaaaatgcg cttcgtggag aactcaatgt tctgctcttt gcagtactta
    18721 gcccatccct taagctcggt gttgttgttt gccattaatt aaagtgcctc ccttaattta
    18781 atgttgattg caacattcgt tgagcgttta cttgtgtgtg gtttcgggca gctgctgatt
    18841 gcccgaagag ggacgtgcgc ggttccttat tgcttggtaa gctggccgcg ccgcgtacga
    18901 cgcggccgat gagttattgt taaaaaagta ccttaggggt gtactttcaa ccttagttgg
    18961 cggcagcctc cgctgcctgc ttgtccttcc acgccttcac aatgtcggcg gcgaggacgg
    19021 gggtgtgata gaacttgacg ccgagcgcgt tgccgatggc ggccatcgtc gcggggatgc
    19081 tcgggatgag cgccggctcg ccgatgccct tggcgccgta cggagcggcg tcgccgtcgc
    19141 tgcacacaac gatcgggtac acctcgggaa catccatgct ggactcgatg atgtacttgc
    19201 tgagcgaggg gttcttgatc gcgcaggtct tcgggtcgat ctcgatcttc tcgctgatga
    19261 cgaagccggt gcccatggcg gtgccgccct cgatctggcc ttcgcacatc ttcacgccga
    19321 ccggcgtgcc gacgtcgtgc gcggaaacgc atttgagaag ctgcacttcg ccggtgtcgg
    19381 tatcgacctc gacctcgatg atgcaggggg cgtaggagnn nnnnnnnnta gacctcatac
    19441 ggaacgccgg acatgtcggc cgggttcaga taggtggtct tcgggttgta gtagcccgcg
    19501 ccgacggcca gacggccgtt tttgcccatc gcgcccatga gctcgggata ggtcatcttc
    19561 ttggactcat ccttggagct gtagatctcg cggtggcgga agatcagctc ttcctgcggg
    19621 acgttcagaa actcggaagc ggtgcgcttg agctcgccca tcgcgttggt gcaggcattc
    19681 ttcacggcgt tgccggtgat gaacgtctgg cgggaggcgg atgtcgcgcc gccttcgggg
    19741 gcgatctggg tatccgccca ggtgacgtgg atgttttcaa actcaagacc gagcgtttcg
    19801 gcggcgatcg ccgcggcgac ggtcgcgacg ccctggccga tgtcggccgc gccgatggta
    19861 acgttaacgc tggtgtcggg cagaacttcg cagaacgcgg cggccgggtt cggaagaccg
    19921 gtgttgccga cgccgtagaa catgctgccg acgcctgttc ctctcaattt catatctgcg
    19981 gcctccaatc cgggtcgaga cgctcaaggg cgtccggcat gacttcctcg gccttcttga
    20041 tcgcctcggc gagcgtgtcc tgatagccga cgctctcggt gagggtctgg ccggtcggga
    20101 tcgtcgagcc gatgtggtgg ccgttgatgc ggcggatctc cagcgggctc atatgcagag
    20161 ccgcggccag agcgtccatc tgtccctcgt ggacaacggc ggcctgcggc acgccgaatc
    20221 cgcggaacgc gccgcacatc gggttgttgg tgtaagcaaa taccgcgtcc agacggacgt
    20281 tcgggcaatc gtaggggccg acggcgtgga ccgggaaacg gccgataacg gcggggccgt
    20341 aggagccgta agcgccggcg tcgcacgtga gctcatactg cgcagccacg agcttgccgt
    20401 ccttcgtcgc gccggtcttg acatgggtcg taatcgggtg acgcttggag gagatcatcg
    20461 tggactccac gcgggagcgg acgatcttca cggggcgctt ggtgtaatgc gcgagcagcg
    20521 cgcagtgcag ctgcacgccg atatcgagct tgccgccgaa gccgccgccc gtggtgggct
    20581 ggatgcagcg gacatggttc tgcggaacgc cgagcatggc ggccacttcg ttgcggtcgt
    20641 agtgggcgtt ctgcgtggag gcgatgaccg tcatgatgcc cttctcgtca tagtaggcga
    20701 tgccggcatc cggctcgatg aacatgtggg agaggacgtg ggtggagtag gtgttctcga
    20761 cgataacatc gcacttgctc caggcttcgt ccacattgcc gaactccatg tgcttcttgg
    20821 catggacgtt cgtcgtcccg tggacgcaga tcgcgccctc cgccatcgcg ctctcgaagg
    20881 agttgatgac gggcagctct tcgtattcga cctcgatgag ctgcatggct tcctgcacaa
    20941 tctccggcgt ttccgccgcg acaacggcga tggcgtcgcc gtagcggcgc accttgtcgt
    21001 ccacgaggca gggctcgtct ttgacgatga tgccgaaacg gttcttgccg gggatggcgg
    21061 tatggtcgag aacggcggcg acgccgggca gcgcgcgggc cttctcgaga ttcagctttt
    21121 tgatgtaagc gtgaggaacg gtgctgcgga acacgccgcc gtagagcatg tcgggcatgt
    21181 aatagtccgc ggcaaacttc gcgcggccga gcaccttgtc gagcgagtcg accttgatat
    21241 ggtcacggcc gaggatagct acgtctctca tgcttcggcc tcctctccgc gcattctggc
    21301 agcggccaga agcacagcgt cattgatctt ggcatagccg gtacagcggc agaggttgcc
    21361 gctgatggcg agcttgacct catcgcgcgt cgggttgggg ttcttcttga gaaaagcctc
    21421 ggcggtgagg atcatgccgg ggatgcagaa gccgcactgc acggcgccgg cgtccatgaa
    21481 cgcctgctgc accacgtcgg gccggccgtt cttgctgacg ccctcgagcg tgatgatgtc
    21541 cgcgccgtcg gcctgtccgg ccagaatgca gcagctcgtc acgggctcgc cgttgtaaat
    21601 gacggtgcag gcgccgcact cgccctcgct gcagccttcc ttgacgctcg tgaggccaag
    21661 gttgttgcgc agcacgtcga tcaggcggtg gttcggctcg acctcgacgg tcaccggctn
    21721 nnnnnnnnnt cgtggttaac ggtgaaatga atggtgatct ttttcaaaat ccctgcacct
    21781 cctcgatatt cagtttgcgg agcacatcgg cgaacaggtg cttgtacaca ccctggatgc
    21841 tctccttttt gtagaacaca gagggacggg acttattcgc ttcgtaaacc tgatcgtgca
    21901 tgatcggcag gcacccgagc atggtctccc agctgagctt cttgccgacg agatactcct
    21961 cgcagctctt gtagcgctgc ggataacgcg ccatggcgcc cgcccgcatg gagcagtagg
    22021 tgcaaacgcc gttatcatcg accgtcacga ccatgccgcc gccgatgacg agcgcgcggc
    22081 gcttgcccag cttataaaac gcggtagcgg tgtgcttcgt atcgggcagc gggaacgaga
    22141 cctcggtcat gagctcgtcc ggctgcagga tgttgcgctt gcggtcggga gaggaaagga
    22201 attcgttgat gttgatcgtg cgctcgccgc gaacgctctt gatggtaacg tcggcgttga
    22261 gcgtgacgca cgcggccaga ccgtcgcccg ccacggaaga ctggcagatg ttgccgccga
    22321 gcgttgcgag gttgcggatc tggggcgagc ccacctgacg gcaggcgtca tagagaatgt
    22381 ggacattctc gcggataatg tcgttggccg ctacctctgc atgagaagta agagcaccga
    22441 tccggacgat gccgttctcg accttgatgt aatcaagctc cttgagcttc ttcaggtcga
    22501 taacgacagc cggctgtgcc ttccactcgt tgagctccag cgtaaggtcg gtgccgccgg
    22561 cgatgcaggc gacgctgtcc ttatactgnn nnnnnnnntg ctgctttcgc tgctttttct
    22621 ccgccggagc gggcggctgt gccgcccgtc ccggttagaa tactttattt tactccagtt
    22681 cgccgcggat ttcaacaccc atcagctttt cccagagttt gataacggcg gacatatctt
    22741 ccatgccgta gccggcggcg cgggcctgct cgtaaacggc ggtgcaggtg gcgctcatcg
    22801 ggatgggcat cttgacgccg cgggcggctt cctgcgcgag ggtcaggtcc ttgtactgca
    22861 cgtccatcgc gaagcccggc tcgaacttgt cgggcatgat gaacttcttc atcttcgcat
    22921 cgacaacgta gctgttgccg gaggaggtgg agataacgtc gtacatggtc ttgttgtcca
    22981 gaccgagctt cgcgccgagc acgagagctt cggcgagaga ggccatgttc gcgccgagca
    23041 gcatgttgtt gacgatcttc atcgcgtcgc ccatgccggt gccgccgatg cgcatgatgc
    23101 gcttgccgat ggcttcgaga acgggcttgg ccttctcaaa ggtctcctcg tccgcgccgc
    23161 acataatggt cagcgtgccc ttctgcgcgc cgatggtgcc gccggaaacc ggagcgtcca
    23221 tgtacttcac gccgtgctcg gcggcgatct tcgccatctt ctgggtggtg ccgggagcga
    23281 cggaggacat gtcaacgatg atggtgccgg gcttgcagtg ggcgagaacg cccttggggc
    23341 cgcacataac ggcctcgacg atcgcgccgt tggggagcat ggtgaagccg atgtccatgt
    23401 tggcggcgac ttcgaggttg tccttgcagc cgatggcgcc ttcggcgacc ttctgctcga
    23461 cgagagcggg aacaaggtcg ctcacgtagg tctcaaaacc agccttgata acgttgctgc
    23521 acatatggcc gcccatacgg cccagcccga taaaaccgac tttcattgga aaagatctcc
    23581 tttaaaatat ttgcaagtta ttgttgccga atgttattgc gcgctgcgct cgaacgtgag
    23641 acgctcgccc tggatatccg cgtatacgcg gacgagattg ccgtttttcc actggcgctc
    23701 cgtctcctca tacacctgca tgagaccgcg ctttttctcc tccgggagaa gcggctcgac
    23761 ggccgtgacg atacggagcg aggcaatgtc gctctccggc tcgatctctc cggcgagaca
    23821 ggtatgtacc cgcggcgatt cgatgtagga gcagtttgcg atgtgcgtgg cgcaggcgtc
    23881 cgcgagagcg caccgccggg aaaagaccgt gacgcctccg gcgatgccgc gcgtgaggct
    23941 ccgcccgccg annnnnnnnn ncccgccgag accgctcgtg cacacgccgc cgatgccgtc
    24001 ctccgcgcag atctccacga tctccgtaac gcggccgttg agatcgggca atatgcccat
    24061 gcggatggag cgtccctcgc cgagccgcag cgccacgtcg ccgccgttat tcacggcaac
    24121 gagatccgcg ccgcgggcga agagccagtc cgccatggcg tccgccaccg ttccggcaac
    24181 ggccgccata ggcgtgagcg tagactcgcc ggtcgcaagg accgattccg ccatgacgcg
    24241 gggcagcccg gtgagttcgg aataatccat gccgtccggg tactgccgca atatgggcag
    24301 cgccccggcg atctcggaga gcgagtcgcg cagaaacggg aaagccgacg ccgccagatc
    24361 actgaggttt tcgtctccgc gccgggcggt caccaccatg gacgccgggc cgtaatcgaa
    24421 gaatacgcgc ccgtcatcca ggatgcggaa ggcttcgtcc atcagtgctt ctgatactca
    24481 gggaagtcct cgacgttctt gatgcagttc atgtggccgc cgatcatcgc gtagtcttcc
    24541 ttcgtcatgg tgtactccac gggggcgacg gtcgccgggg tgggcaccca ggtgaaggcg
    24601 tggttgacga ccttcgcggt gtcaacgatg aagttgatgc cgccgccggg gtaaacgtaa
    24661 gcgggagcgc cgccgatggt caggtgcgcc ttgccggcgt gcacggcctc ggtgatggcg
    24721 atcgggtggt ggcagacgcc gccgcgggcg gagccgccgg tgccgccgca gtacataacg
    24781 ctcgtgttgg agccctggca gttgctcttg atggcatcga cgacgcgctg cgccttctcg
    24841 ctcatcggga tctccttcac gtcgccgtcg gcctggacct cgaagagggc gccgacttcg
    24901 ccggtggtgt tggtgacaag gatggtcatg ccggcatggg caacgctcat gtcaacgctc
    24961 ttgatggcgt cgcgcggcgt ctggagaacg gtgcctccga tgccgtcgcc gtggccgccg
    25021 aagtaacggc cgacggtgct cttggtggcg ttggggataa cgccagacca ggtcatgccg
    25081 acttccttgc cggcgagatg ctcgctgaag aggccggtga cgtggtggtc gatgacgatg
    25141 cactcgtcca cgcactcctt catgacgtcg gcgagcagac cgatggtggc gctgccgcag
    25201 ccgatacgca tggtggtctc ctgcgtgccg ttgatgatgg gagcgacgcc ctgctggcag
    25261 acgatcttga tcatcttggg cttgccggtc tcgtggtcgg cggtcttgac ggtcagctcg
    25321 accttctcgc cgttggcaag agcgacaatg gtgcgggcgg tggcaaagcc gccgtccttc
    25381 gcggtcagac ggttcgcgcc gccgacgtag atcatcttgg agccgtactc ctcggtatgt
    25441 acaagaccga cgggcttgcc ttcgcagtaa acggtgtcgc cctcttcgcc gatgtaggtg
    25501 ttggtgtcga gcttgacgag aacgccggag tagctcagcg gagcctcggt gacggtggtg
    25561 acaacgtcaa cgccgtcgcg cttctcgcag acgatgtgcg gagccggacg gatgcagggg
    25621 tagttggtgc ccgcgccgac agcggtgatg acgggcttgg caattctcgg atcgatctcg
    25681 atctggggct tgccctctac gacgagcgcg cggttgcgga ccagacggcc gccctcgttg
    25741 gtgtagcgct tgcaggcgcc ggtggagcca acgggaactt tgcactggat ggagcaggaa
    25801 tggcactgca ccatgccctc ggtcttctcg gcgccgtcga tggcaccgaa cgggcagacg
    25861 cggcggcaga cgttgcactc aacgcacatg ttcgtgatgc tgacgacttt cttgccgtcc
    25921 gcacccggtt caacggcaat cgcaccgatg gggcagttct tcgcgcaaat ctggcacttc
    25981 ttgcattttt cctgattgat tttcagcatg tcacgctctc cttttaataa tgtataccta
    26041 tcccatgcgt tggataatct ctcgtatata ttattatcgc aaacccctat ttttttcaaa
    26101 tacattattt gtatgaaatt tgccgtattc atatccaaac tgtagcgaag ggtctaatat
    26161 tcgccgaaaa tcgcatcttg attttgtgca gaataaacat tcggtaaaac cattttgaca
    26221 actattgcaa tttgtcgtct ttttttgcat aatacttcta cagtttgtat gccgcataaa
    26281 gcggcatcac gccggaaaag agggcgaaaa tggataacaa aatggatatg cgcgccgtcg
    26341 cgtgcgtcct gctggcagcg ggagcgggct cgcgcttcgg cggcggaaag ctgctcgcgg
    26401 aatttggcgg cgaacgtctt ttcgagcgcg cgatgaacgt tctgccaaag gagcttttct
    26461 cgcgcgtcgc ggtcgtgtcc ggctgtgagg atattctcgc cgagagcgag cggcgcggct
    26521 ttctcgccgt gcgcaacagc gccccggaga agggcgtttc cctctctatc cggctgggtc
    26581 ttgaggcagc aaagccttgc agcgccgtgc tttttctcgt ctgtgaccag ccgctcctcc
    26641 ggaaagaaag cgtgctccgg atcctcgaag cgggcgcggc gccccccgaa aggatcattg
    26701 tgccggaagg cgcggacggg cggctcggca atccctgcct gttcccggca gcctattttt
    26761 cggagcttgc cgcgctcgag ggggaccgcg gcggaaagcg cgtcatccgt gcccatccgg
    26821 aggc
//
LOCUS       DAWAAE010000011        26574 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_793_length_26574_cov_3.605000, whole genome shotgun sequence.
ACCESSION   DAWAAE010000011 DAWAAE010000000
VERSION     DAWAAE010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 26574)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26574)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26574
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_793_length_26574_cov_3.605000"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15298..15307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22158..22167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4771 a         8208 c         8686 g         4889 t
ORIGIN      
        1 gtcggagccg aaggggaagg tgcgcagcag gaagaagcgc agcgcgtcca cgccgtagcg
       61 ctcggcgagc atgtagggat cgacaacgtt gcccttgctc ttggacattt tgccgccgtc
      121 catgatgagc cagccgtgac cgtatacctt cttcggcagc ggcagaccca cgctcataag
      181 catcgcgggc cagatgatcg agtggaagcg gacgatctcc ttgccgacga tgtgtacgtc
      241 cgcgggccag tatttatcga aatcgtggta cttgtcgttc tcgtagccga gcgcggtcgc
      301 gtagttgaag agcgcgtcga tccacacgta cacgacgtgg ccggggtcaa agtccacggg
      361 cacgccccag gtaaagctcg tgcgggagac gcagagatcc tgcagcccgc cctcgcagtc
      421 gatgaagtta tggaccatct cgttcacgcg gctcgcgggc tcgagaaact cgccgccggt
      481 cagaagcgag cgaacgcggt cagcgtactt gctcagacgg aagaagtagg cttcttcctc
      541 ggcgtacttg acctcgccgc cgcagtcggg gcagcggccg tttacgagct ggctctcggt
      601 ccagaagctc tcgcacggcg tgcagtacat gccggaatac ttgcccttgt agatatcgcc
      661 gttgtcgtac atcttgcgga aaatgcgctg gatgctcttg acgtggtaat cgtccgtggt
      721 gcggatgaag cggtcgttcg agatgttcat cagcttccag agatcgagca cgcccttctc
      781 gccggtgacg atgttgtcca cgaactgctg cggcgtgacg ccggcggctt tggccttgtc
      841 ctcgatcttc tggccgtgct cgtcggtgcc ggtgaggaac atcacgtcgt agccgcacag
      901 gcgcttgtag cgggcgatcg cgtcgctcgc gacggtgcag taggcgtggc cgatgtggag
      961 cttcgcggag gggtagtaga tcggcgttgt aatataaaag gtcggttttt ccataagact
     1021 tctctctcct gactttcttt tatgctcagc cctgcgggac gttgcgggct tcgatgtcct
     1081 cggtgtggta attgtaggtg ctgtaggcct cgtattcctt gctgatgggg gtggcggtgt
     1141 tgtaatcgtc gccgtcgttg aacaccagcc gccacgttgc cgccgccatg ccgacggaaa
     1201 gcggattgcc gttggcgtca aaatacgtgc cggtctcgtc gaagacctcc cagttgttga
     1261 actgcatgag cacatactgg tgcttcacat cggtgccgag gatctggacg cagcactcgt
     1321 tcgtctcctc gtctacccac gcgtggatct tgacggggta ctcggcgtca ttgcggaact
     1381 tgaagtccgg ccagccccag ctcaccgtgg cgtccatgcc ccaggggaga tagcccacct
     1441 ggaactggtg gcaggcgcgc tcgacgatct ccagattggc gtagagcacg gcgttgtaga
     1501 gcgtggagct cacctggcag atgccgccgc cgtattcctg ccggacctcg ccgttggcgt
     1561 aggccgtggc gggcttccag ccgttggctt ccgtgcgctc gccgagcgtg tcgttgaagg
     1621 agagcgtctg tccggggatg agcaccagcc cgtcgagcag cccgcacgcc ttctttacgt
     1681 tggaaatgcg gtcagcgctg ctgccggaga gtgatgtgcg cacctcgttg cagtagtcgc
     1741 ggttccagag ccggacgagc gtccagttct tggagagcgc gtcgcggaag agcatgtcgt
     1801 tgatctcctc cgtggtgtgc tcgggcttcg tgacggtcat cgggatcgtg actgtgtcac
     1861 cgtaggatgc cgcggcccag gccttttcgg cttcggctac gtcaaacgtc cggccgacgg
     1921 aggactgtgt cacgatgaac ggctcgccgt ggaagtcgat ctgcgaggga tcggtcttgc
     1981 tgcccgccgc gggcgtcgcc tccggggagg cggaggcctc cggcgtgtcg gacggcgcgg
     2041 gggtcgcggt gggttcgggc gttgccgtgg gcttttcgcc cttgccgtcg tccttgccgg
     2101 ggtcggtcgt ctcaatgtca gggttataga cgatctccgc ttcggcaacc tcggaataaa
     2161 tgtcgttata gagcgactgg aaatcgaacg attcgtccgt gttcggcgcg ctctcgtagg
     2221 tgaacgacgt gctgccgccg gagaggaacg catcgcggaa ggagttgaac accgaatcag
     2281 tgtcgagcgt catgccgctc gcgcccttga gaagggtgat gctgtcctcg ccgacgacga
     2341 tgctgctctc gagaagctgc gcgtcgatgt ccacagcttt cgcctcgatg atggagcgca
     2401 tcttcgcctc gtcgagcgtc acgtcaaaaa gctcgccgtc gtacgcaaag ccgcacttgc
     2461 cgagatagcg gcactggaca tatttcacca gcgcgccgaa gagcccgtcg ccgcggccgt
     2521 agttccaggc ggcgtctgcc gcggtgtcgg cggaaaagcg catgcctgcg tctgcggagg
     2581 acatggcgag cgtgttgccg agcgggaggt ttgccgtgac ggagtaaccg tcgtagcgct
     2641 cgtggccgag cacaagcagc ttatcccgcg cctcattgcg cgtcatgccg gagagatcgg
     2701 tgtcggcgat cgtcacgccc ggatagatcg tatccacttt gtgcacgagc agcgcgcctg
     2761 tcacgacgag tgcgagcaga agcagcagca cgacgcacac gatgatgacc ggcagcttcc
     2821 agcccccgcg cggcgcgggc gcatccggcg tgatgggcac ttcgctcgcg cggcgggagc
     2881 gctttttcgg cgctttctcc tccgggtctt cgcgctcggc ccggcgcgcg cgcaccggca
     2941 gcggcgggag atccgccggg ggcgcggcgt attcggcctc gcgtttcgcg cgctttgcgg
     3001 cgcgggaatt tttctcccgt tccgccgcgg cttcacgctc acggcgctcc ttgcggctga
     3061 gcgtgcgcgg cgcggtctcc ggctcgccgg tcacggtctc gcccgtatcc cggtcggcgg
     3121 aatcgtagct ctcgccgacg atctccggct cgggctcata ttcgttatat tcggtattcc
     3181 ccgtctcctg cggctcttcg tccgggaagg ggatgtaatt ctcgtcgtta cggatgatgg
     3241 gtaccgcctt tctctgtttg cggctttcgc ctcagttcag ttcatatacg gctcggacgc
     3301 cgtcggcagc gggaccttct gtccctttgc ctcaaacgcc tcgcggatcc acgggttggc
     3361 gttgaatttc tctttcgtct gcgattcgag cagcacgccg atgaggacgt tggaaacgag
     3421 gaatccgccg ggcgtgaagt gccagcggcc gttgtcgcac acggcccagc cgttttcctg
     3481 aaatacctcc agcatctctt cgagcttgtc gaagctgctc tggtacacgc tccggtactc
     3541 ctcgcgggag atgccggccg cggtgcgcat gccgagcatc agatactcgc tcgcgcggtc
     3601 gaacggcgtg agctcctcct cttcggcgat aatacccttg tcccccaaaa cgccggaaat
     3661 atactcgcgg acgttgtggg tgtaggtata gcgcagcagc ccgatgttgg aggccgccgc
     3721 cgcgccgaag ccgatgtagt catccagccg ccagtacttg agattgtggc ggctctcgta
     3781 gccggggcgg gcgaagttgg agatctcata ctgccggaag ccgtggcgcg aaaggaagtc
     3841 cacggcgtaa aggtacatgt ccgcctgatc gtcgtcggag gggagaatgt cggtgccctc
     3901 atactcgtcg tacatgggcg tgccttcctc aagacgcaga ccgtagcagg agatgtgcgc
     3961 gggcttgagc gcgagcgctt tggtgagcgt gtcggcccag tcctcgcgcg tctggctcgg
     4021 cagaccgtag atgagatcga gactcacgtt gcggaagccg gccttgcgcg cgcgcttgac
     4081 cgccatttcc acctgccgcc agttgtggcg gcggccgatg agccggagaa tgtcgtcgtt
     4141 ggcgctctgc gcgccgatgg aaaggcggtt cacgccctcc ttgcgcagca tgcgcagctc
     4201 cttgcgccgg acgctgtccg ggttgcactc cacggtgatc tcgctctgct tcatcagccg
     4261 gttcgtgacc ttcagctcct gcaatatctc tacgatgcgc cgcgcgccgt agtagctcgg
     4321 cgtgccgccg ccgaagtaca ccgtgtcgat aaagtaatcg gtcatgcggg gcatcgtttc
     4381 ctgcaattga atgagcagcg catcctggta atagggcatg agcttgtccc tgcccggcag
     4441 actgcagaag tcgcagtacg cgcacttcga tgcgcagaac ggtatgtgaa aatatacgcc
     4501 caggcgtttg tcttggtcgc tcatagttcc tccagaatgt gggtggtggg tgcggggaaa
     4561 cccctccgtc aaaaccttcg gttttgccac ctcccctgtt aggggaggca agtgggtttg
     4621 cagaatacta acttggctcc cctaacaggg gcagactctc cccggcgggg agagatgtcg
     4681 ccataggcga cagagagggg ggcggggctg gcgcggcgca gccgtgactg aggggtttgt
     4741 ttgaattcgt cagaatagtt tgcagacaag ctcgccatac tccgccggat cgtccggggt
     4801 gatgccggcg acgagctgcg cctgcgcgaa gagcaccttc gccataacgg cggcgcgctc
     4861 cgggttctcc tgacgcgcgg cgtcgagcgc tttgacggcg gggtggtcga gattgagctc
     4921 gagcacgcgc tgcgcgcgca tcgcgttcgg gtcggcaccc ggcatggcgt ggaagtaccg
     4981 ctccatttcg agcgtgatgc cgccctcgac ggagaggcac acggcgtaat tcttgagctt
     5041 gcgggagatc ttcacgttcg ccacggcgtc gccgagcgtc tccttgacga aatcggtgag
     5101 gggcttaaac tcctcgtcgg tcttgtcggc ggcggccttc tcctcgtcgg actcgagacc
     5161 gagatcctgc gagcagacat tgcagaactt cttctcggca tagctgccga ggatctccgc
     5221 gacgaactcg tccgcgtcgg tcgagaagca gaggatgtcg aagcccttgg cgcgcacctg
     5281 ctcggtctgc gggagcttcg cggcgcgctc cggcgtttcg gcggcggcgt aatagatgta
     5341 tttctggttt tccggcatcg cgtccacata ctcgcgcagg gagacgagct tttccttctc
     5401 cgcgctgcga tagagcagaa gatcggtcag cagatcgcgc ttcatgccgt aatcgccgat
     5461 gatgccgtat ttgagctgcg tgccgaacac gttgaagaac ttttcgtact tctcgcggtc
     5521 gcactccatg agccgggaaa gctcgccctt gatcttcttt tcgagattca tggagatgag
     5581 cttgagctgg cggtcgtgct ggagcatctc gcgggagatg ttcagcgaaa gatccgggga
     5641 atcgacgatg ccgcgcacaa agcggaagca ttccggcagc aggtcggggc acttttccat
     5701 gatcatcacg ccattggagt agagccgcag gccggggaca tagtcgcggg tgtagtagtc
     5761 gtagggcgcc ttcgccggga cgaacagcat cgcgcggtag ctcacggtgc cctcggcgtt
     5821 tacgcggatg acggacacgg ggtcctcggt gtccttgaac ttctccttat agaaggcgat
     5881 gcagtcggcg tcggaggctt ccttcttgct gcgctgccag atggggatca tcgagttgat
     5941 gacctcttcc tttgtgacgg tgcgcacgcc catcttctga ataccggcct cgtccacctc
     6001 gccggtcttg tatttctcgg tcgtctccac gtcggtgcgg atcggccagc ggacatagtc
     6061 cgaatatttc ttcacaagct cctggatctt ccagggcatg agatacacgc tgtagcagtc
     6121 gtcctcggtg tcgctcttca tggtgaggat gacgtcggtg ccgacggtct ccttctcgca
     6181 gggggtgatg gtgtagccgt ccgcgccctc gctctcccag cggttggcgg tctcctgccc
     6241 gtaggcgcgg gagatgacgg tcactttgct cgcgaccatg aatgcggaat agaagcccac
     6301 gccgaactga ccgatcacgt cgatgtccgc cttgtccttc tcggcggcgt ccagatcctt
     6361 cttgaaggcg agcgagccgg acttcgcgat cacgccgaga ttggattcca tctcctcggc
     6421 ggtcatgccg atgccgttgt cggagatcgt gagcgttccg ttttccttgt cgcccttgat
     6481 gcggatgaag aaatctccgc gctccatgcc gacattctca tcggtcagcg agcggaagca
     6541 gagcttgtcg atagcgtccg aggcgttgga gatcagctcg cgcagaaaga tctcctgatg
     6601 cgtgtagatg gaattgatca tcagctccag cagccgccgg gattccgcct gaaattcttt
     6661 tttcgccata tttttatcct ccgtatgcta tggactctga gcagaccttg agaaggccta
     6721 tacccagaca ctatattata ccgcggcatt atgtaaaatt caaccgaaaa actcgcccgc
     6781 cgcagaggaa aagaggctcc gcgggggaaa atcgtgttcc cgctgccgaa accggcgcac
     6841 aagaagtttt attttcggca ggacatggcc gcggggtgcc ggagcgcgga cgatccccgc
     6901 ggcactacgc tgacgcaggc gttctatatc ccgggggatc agggggtcgt actggattat
     6961 aaggacggac tttatactcc gcgaaatccg gcgaccgcat ttcggagctg tacggcggcg
     7021 tgtgcatggc gcttgcccac acctctcccg gacagcatta tcccaacgtg tttgacgacc
     7081 ggaacaagtc atatgccatt ccggcgggcg tgaaatataa gctgctccgt caggagaccg
     7141 gcgagagctg ctatatcctg acagagtatc cgctggacag atcgcagccg gactggacga
     7201 ccttcgggga gccataaaac cggcaggtga atacgagcaa gaaaaatttt cgcccgcaga
     7261 cattgtctgc gggcgaaaac cgtttggatt tttatttctt ttccggtatc tgcgaatggc
     7321 cggcgtttac gagaaggtca tgccggatgt cccacaggcg gttcgagaga tcgacgtagt
     7381 acccgtgcgg gttttcaaac cggcgcacct cgggccagag cgtttccagc tgctcggcgc
     7441 agcgcttccg gatatccttg agcgacggga gatcatagac gagctcgccc gcgcggaaca
     7501 cggggaccat catgttttcg gcgcggaagt tcgtgagcgt tttgcgcttc cacgtgtttt
     7561 ccgggtggaa gatcgtgagc ggctgcgtat cgtccggcgt ctcgtcatac aggcagagat
     7621 agtccgcaag cgccttgccg ttcgtgcggt cgtagaagcg gtaaaacttc ttgaagtggg
     7681 ggatcgtgat cttggcgacg ttttcgctca ccttgatctt cgggacgacc tcgcccgatt
     7741 cgcgctccac ggcgacgagc ttgtatacgc cgccgaacac cggctcgctc ttggcggtga
     7801 tgagccgctc gccgacgccg aaggagtcga tgcacgcgcc ctgcagcagc aggtcctgga
     7861 tgatatattc gtcgagcgag ttggagcagg tgattttgca gctctcccag cccgcctcgt
     7921 cgagcatgcg gcgggcctcc ttcgtgagat aggacatatc gccggagtcg agccggatgc
     7981 cgcatttggt gatgcccatc ggcttgagca cgtcgttgaa ggcgcggatg gcgttgggaa
     8041 tgccggattt gagcgtgttg taggtatcca cgagcagcac cgcgttcgtc gggtagctct
     8101 cacagtaggt gcggaaggcc tcgtattcag aatcgaacat ctgcacccag gagtgcgcca
     8161 tcgtgccgag agcgggaacg ccgtagagct ggtcggagat cgtgcaggcg gtgccggagc
     8221 agcccgcgat atacgccgcg cgcgcgccga ggatcgccgc gtccgccccc tgcgcccggc
     8281 gggagccgaa ttccatcacc gcgcggcccc gcgcggcacg gacgatgcgg ctcgccttcg
     8341 ttgcgatgag cgactggtgg ttcaccgcga gcaggagata cgtctccacg agctgcgcct
     8401 cgatcgcggg ggcacgcacc gtaatgagcg gctcgcgcgg gaacaccggc gtgccctccg
     8461 gcacggccca gatgtcgccg gtgaaccgga aatcgcggag gtactgcaga aactcctcgc
     8521 agaaaatgcc cctcgagcgg agatagtcga tatcctcatc ggagaagtgg agattggtga
     8581 tatattccac gacctgctcc agccccgcgg caatggcgaa gccgccgccg tccggcacct
     8641 gccggaagaa gaggtcgaag taggtgatgc ggtccttata tccgttgcgg aagtagccgt
     8701 tgctcatcgt cagctcgtaa aaatcgcaga gcatcgaaag attctgggca ttgcgaacgt
     8761 tcatggcaaa gtgtcctcct gccggtagta tccatataat tatacacgcc cgccgcattt
     8821 ttgcaaggcg attgacacgc gcgcggaaaa atattatttt atagtgtgtg aaattataaa
     8881 ctatagatac aggcgggaga ctatggaaaa cgcgccggga aaagagaata cgttcccgcg
     8941 gctgtacgcc ggttccggac gcgtgccgga ggacacggcg cggcgtgtcg tcttcgcgtc
     9001 gtgggcgctg ctcgagccga cggagggaca cacggatgcg cccgccgcgg ctgccgtgcg
     9061 cgaagagctg atgcgctgca tcgcccgcgg cgaaccgccg gttttgtgcc tgtacgccgg
     9121 ggaggatccg gcatggttcg ccgcgaaggg cggctggctc gcggaggaca atctccgctg
     9181 cttcctgcgc tatgccgggc gcgcggcgcg cgcgttcggc catctgacgg acgaatacat
     9241 cacgctcttt gagccgaacg agcttgtttg gaaaaagagc gccaaccgca atctcaatct
     9301 gcgcttcaaa atgctttcgc acatggcgtg cgcccatgtg cgcgccgtga agctcgtgcg
     9361 cgatacgcgc gcgcagcggc agctgccgga aacgcgcatc ggcttcgtgc tgcggatgta
     9421 cccggcgatc gatctccggc gggggctgct gcgcggggac aacgccgcga ccgcctcggc
     9481 gtatgaaata ctgccgctgc tcgccatggc gcggggcaat tttctcccgc cgctgcgcaa
     9541 tacgctccgc attcggcccg gaagctgggc ggatttcctc gccgtttccg gcggcgggga
     9601 caaagagaag cgccgctact gctgccatgc cgccgccacg cttgccgaaa cggagatctg
     9661 ggaggtcatg aatgaccggg aggacacata aaacggatac aaaggatttt ctgcctgtct
     9721 cgcgcgagga tatgctcgcg cgtggctggg actggtacga tatactgctc gtgacgggcg
     9781 acgcttatgt cgatcacccg tcgttcggcg cggccgtcat cgggcggctg ctcgagaacg
     9841 cgggctaccg cgtggcggtg ctcgcgcagc ccgcgtggaa cgacgcctcc gcgtttgcgg
     9901 cgatggggcg gcccgagctc ggcgttttca tcggcgcggg caatctcgac agcatggtcg
     9961 cgcactatac ggcggcgaaa aagcgccgca gcgaggattt ctattccccc ggccgtaagg
    10021 ccgggctgcg gcccgaccgc gcctgcaccg tgtacgccaa ccgcgcgcgg caggcgttcc
    10081 ccggcatccc cgtcctgctc ggcgggatgg aggcctcgct gcgccggttc gcgcattacg
    10141 attactggga cgataaggtc cgccggagcg tcattttcga cgcgcgcgcg gatatccttg
    10201 tctacggcat gggcgagagc gcaacgctcg aggccgcaga ccgcctgcgc gacggcaagc
    10261 ccctgcgcgg catccgcggc acggcggtca ttgccgataa agcgccggag ggcgcggtgc
    10321 tcctgccgtc gtttgaggaa gtgcgggacg atacgaagaa atacgctctc gccacgcgcg
    10381 cggaatacgc cgagcacgac cccatccggg gaaagacgct cgcgcaggcg cacggcgata
    10441 aattccttgt cgtctacccg ccggccatgc cgctcaaaac ggaggagctg gactcggtcg
    10501 cggcgctgcc gtatacgcgc gagccccacc cgatgtacga tcccctcggc ggcgttccgg
    10561 ccattgagga ggtgcgcttt agcgtcatcc acaaccgcgg gtgcttcggc gggtgcaact
    10621 tctgctcgct cgcgttccac caggggcgga tggtcacatc ccgcagccac gaatccgtca
    10681 tccgcgaggt ggagcagatg acgcaccatc cgctctggaa gggctatgtc tccgatgtcg
    10741 gcggcccgac ggcgaatttc cgcggcccca gctgcaaaaa gcagagcaaa gacggcatgt
    10801 gccctctgaa gcactgcctt gcgccgacgc cgtgcaaaaa tctcgttgcc gaccaccgcg
    10861 attatctcgc gctgctgcgg aagctgcgcg ccgttccggg cgtcaagaag gtgtttgtgc
    10921 gctccggcgt gcggtttgac tatgtcctct gcgacaagaa cagcccgttc ctctcggagc
    10981 ttgtgcagta ccacatctcc ggccagctca aggtggcgcc ggagcactgc gtcgatacgg
    11041 tgctcggcta tatgggcaag ccgccggtcg cggtatacga acggtttctc gataaatacc
    11101 acgcgctcaa cgcgcgctac ggcaaggcgc agttcgtcgt gccgtatctc atgtcctcgc
    11161 acccgggcag ccggatggag gacgcggtgg cgctcgcgct gtatttaaag gcgcacaacg
    11221 cccgccccga gcaggtgcag gatttttacc cgacgcccgg cacgatcagc acctgcatgt
    11281 accacacggg catcgacccc atcacgatga aggacgtgta cgtcccgacg gacgcgcacg
    11341 aaaaggcgat gcagcgcgcg cttttgcagt ataccgaccc caaaaacgcc cggctcgtgc
    11401 gcgaagcgct cgcctttgcc ggacgcgggg atctgatcgg ttacggcgga aagtgcctcg
    11461 tgcgcccggc gaagggcgaa aaaccgtctg accccggcga aggaaaggaa aaggaccatg
    11521 gaaacaaaaa gcatcctcct gttcgcgtat ctgctcggaa tgaacgcggc ggcgtacatc
    11581 ctcatggcgg cggacaagac aaacgccatg cgaaaggcgc ggcgcattcc ggaaataacg
    11641 ctccttctga tcgcggcgct cggcggaagt ctgggcgagt ggctcggcat ggtaagcctg
    11701 catcacaaga caaagcataa gaagtttctc atcctcgtgc cggcgtttct gtttatccat
    11761 atcgcgctcg gcgcgtggct gctctttttc cggggataaa agaatatgtc gaaaactgtc
    11821 gaaattacgg atttctcttc cccggcgctg gacgtttacg cccgcctgac ggaaaaccaa
    11881 ctcgtgagcc gcgccgaccc tgcaaacgcg atgttcatcg ccgagagccc gctcgtcatc
    11941 ggtcgggcgc tcgatgccgg gtatgagccg gtgtcggtgc tcatggaggc gcggcatgga
    12001 aacggcgcgg gcgcggagat cttagcgcgt ctcggcgcgg acgtgccggt atttaccgcg
    12061 ccggacagcg tgcttgtgaa tctcacgggg tttcacctga cgcgcggcat gctctgctgc
    12121 atgcgccgcc gggcgctgcc gccggcggag gcgctctgcc gggacgcgcg gcgcgtcgcg
    12181 gtgctcgaaa acgtgatgaa cccgacgaac atcggcgcga tcttccgcag cgcggcagcg
    12241 ctcggcatgg acgccgtgct gctcaccgag gccgggagcg accccttgta ccgccgcgct
    12301 gcgcgcgtga gcatgggcac ggtttttcag gtgccgtgga cgtatctccc ggcgggggag
    12361 cgctggcagg atacgctgca cgcgctcggc tttgaaaccg cggcgctcgc gctgcgggac
    12421 gacagcctga gcatcgccga tccgcgcctt ggaagggtcg aaaagctcgc gctctgtctc
    12481 ggcacggagg gcgacgggct cgcaaacgat accattgcag actgcgacta taccgtgcgc
    12541 atccccatgt cgcacggcgt ggacagtctg aacgtagccg cggcgagcgc cgtggcgttt
    12601 tatcagctcg gcacgctctg ccgtaaaacg gaagaatttt tatgaaaaga accgcaaaga
    12661 tgctcaccgc ggcgctgctc gcgcttacgc tcgcgctgcc cggcaccgcc gccgcggcgg
    12721 acgatacgcc ggcggtgcgc atctccgaaa tgatgtataa aaaccacgcg acactccagg
    12781 acgccgacgg cgatttttcc gactggttcg agctggagaa tacgtcgaac cgcgtcgtgc
    12841 gcctcaaggg gtggagcgtg tcggacggaa agaccgtctg ggattttccc gcggacgcga
    12901 cgataccgcg cggtggggtg cgcgtcgttt tcgcctcgcg caaggataag accgcggcgg
    12961 gcgaaagcca cacgtcgttc gcgctcggcg agggggagac gctgtatctc atcgcgccgg
    13021 gcgggacgat cgccgaccgc gccgcgtgcg atcccgagct gcccgccgac catgtgctgc
    13081 ggcgcgaaaa cggcggcgag ctcaccgaaa gtgtctgggc gacgccgggg tacccgaaca
    13141 ccgccgcggg gtacgcggcg ttctgcgaga gccgcaaaac ggaaagcccg ctcgtgatct
    13201 atgaggcggc ggtctataac gacacgttcg ctctcaaggg cgagtattac gactgggtcg
    13261 agataaagaa cgtatcgaaa gagagcgtga agctctccga ctactgcctt tcggacgacc
    13321 ggcgggagct ggaaaaatgg tcgcttccca accggacgct cgcgaaggga cagtcgatcc
    13381 tcatctactg caccggcgag gagaacccga cgaccggcaa cggcctgcgg gcgaatttcg
    13441 cgctcaacgc ttcgagtgaa acgctctatc tcacgaagaa gggcgagagc gcggcggcgg
    13501 acttcgtctg gctgcacgat atcccctacg ggtacagcat gggccgcgcg gacgccgaaa
    13561 acggcttttt ctatctccgc aggcagacgc cgaacgagcc gaacgagacc gacgcttacc
    13621 ggtatatctc cgaagctcct gcggcgaacc gcgaatcggg ggtctacgaa gcggggcaga
    13681 ccgtgcgcgt gaagctcgcc gcggcgggcg atatttacta tacgaccgac ggctccgccc
    13741 ccacggagga gagcacgccg tacaccggcg cgatcaccgt ggacaaaacg accgtccttc
    13801 gcgccgctgc cgtggaggag ggcggcgcac cgtcgcgcgc gctcacgctc agcttcttca
    13861 tcggcgagga gcacacgctg ccggtgctca gtcttgtgac cgactcaccc cgacggtttt
    13921 ccgaaacata taacggcgcc gtaaaatacc gcgaatgcgc ggcgaatctc tcgcttctcg
    13981 ccccggaagg cggcttttcc atcgactgcg gcgtggagct caagggacgc accagtcttt
    14041 tcaacccgaa aaagagcctc ggcgtttcgt tccgcggctg ctacggcgcg gagacgctcc
    14101 gatacgacat cttcggtaaa ggcccggcgg agttcaccga gctttccatc cgcgccgggc
    14161 aggactatat ctccacggtg ttccgcaacg agctcatgca ggagctgtgt gccgagctcg
    14221 atacgaccgt gacgcagcgc agccgctact gcgtgctgta catcaacggc gaatactggg
    14281 gcatctactg tctcaaggac gatatcacgc ggcagtttta tgccaatctc accggcacgg
    14341 cgaaggaaga cgtgacgatg ctgccctcgc cggtggcgca gaatacggcg gtgtataccg
    14401 aggcgctcgg actctggcgg gacacctcac tcacacgccg ggaagcctat gagcgcttct
    14461 gcgaggccat ggatatggac ggcttcatcg actgggtgct gctcgagggc tactgcgcaa
    14521 acgtagacat caagagcaat ctccgttatt tccgcaccgg cgacgagccg tggcagatcg
    14581 ccttttacga tctcgactgg gcgttccaga cgaggagcaa ctgctttttc gatctcatcg
    14641 gcccggagca gaccgcgcag atcgccccga cgatccgctg gctcttcggc atcgacgact
    14701 ttaaggagcg gcttctcacg cggtacgccg agctgacgga aacgacgctc tcggacgagc
    14761 acgtcacgga gaagatcgac ggattccgcg cgctgctcgc gccggagatc gcgcgggagc
    14821 gtgtgcgctg gggcggcagc gaggaaggct ggaacgaaca ggtggacgcg ctgcgcgcga
    14881 acattgccga cgactacgcc gcccacactg tccggaacct ctgcagcatt ctcggcatgg
    14941 gcgatgagga acgcatggag tatttcggct tctgacgccg ccgtccggca gacgctctgc
    15001 cgccttgaca aaccgagggc agcagagtag aatccaatca aaaaaagacg cctgagtgct
    15061 ccgtagacag gtaaaaacgc ttcggagaga ataggaaatc cggggaaggt ccggaacggt
    15121 accgccactg taagtgcgga agctccgccg gggcgaaagc cggtcattgg ggagacccga
    15181 gaaggctgcg cggagctgag gatgcacaag tcaggagacc tgctcagaga tgcgtcgctt
    15241 ccccggctat ggggaataca tcatgccagc ggaaaaacgg ccgcggcggt ccaaaagnnn
    15301 nnnnnnncgg cttttctgca tggggaggat cttatggacc gaatcatgat ctccggcgtg
    15361 agcagcggct gcggcaagac gacggtcgtg tgcgccgtat tgcaggcgct ggtgaaccgc
    15421 ggcttgaaga ccggcgcgtt caaatgcggg ccggactaca tcgacccgat gttccacagc
    15481 cgcatcatcg gcgcgaagag cgccaatctc gatctgcatt tcttttcgga aaacacgctc
    15541 cgcttcctgc tcgcgaaaaa cgccgcgggg tgcgatgtga gcgttatcga aggggcaatg
    15601 ggcttttatg acggcgcggg gctcaccaca tggaaaacga gcgcgtatga gctcgccctc
    15661 gttacgaaaa cgccggtcgt gctcgtggtg aacgcgcgcg gcgcggcgct ctcggtgctc
    15721 gcggtcatcg aagggttttt gcgcttccgg cccgagagcg gcatccgcgg cgttatactc
    15781 aaccagtgta ccgccatgac gtacgcttcg ctctccgccg ccatcgagga gcgcttcggc
    15841 atccggtgct tcggctttct gccgcggctc gacgaatgcg tgctcgagag ccgccatctg
    15901 gggcttgtga cggcggggga gatccgagat ctccgcgaaa agatgcagac gctcgccgcg
    15961 caggcggaaa agacactgga tatcgacgct cttctcgcgc tcgcgcacga ggccgagcct
    16021 ctggactata cgcccgccgt gctgccgaag aaggagaaga tccggctggc cgttgcgcgg
    16081 gacaacgcct tctgctttta ttatgaagac agtcttgacg ccgtgcggga gatgggcggc
    16141 gagcttgtgc cgttcagccc gatcaccgac ggcgcgctgc cggagaatat cgacggactc
    16201 tatctcggcg gcgggtatcc ggagctgtac gcgaaggagc ttggcgaaaa cgcctccatg
    16261 cgcgcgtccg tccgcgcggc gctggagcgg ggcgtgccgt gcatcgccga gtgcggcggg
    16321 ttcatgtatc tcaccgaagc catcggcgat gagcccatgg cgggtttcct gccggggaca
    16381 tgcttcgata ccggaaagct cacccgcttc ggatatgtga cgctgcgcgc gaagaaggac
    16441 aatcttctct gccgcgcggg aggcgagatc gccgcgcacg agttccacca ctgggactgc
    16501 acctgccccg gcgacgcctt tgccgccgaa aagccgacgg gacggcgctg ggagtgcgct
    16561 gtggcctccg atacgctcta tgcgggctat ccgcattttc atttctattc caacctggaa
    16621 tttctttaca gcttttatga cgcctgtata aaggagaaac accgccatga tggaaaatat
    16681 taaacccatg gacattgaaa agcgcagctt tgccatcatc accgagctgc tcggcgatac
    16741 gaagctcgag gaggaaaacg agcttgtcat caagcgcgtt atccacacga ccgcggattt
    16801 tgactatgtg cagaatcttg tattctcccc gcacgccgtg acgaagggac tcgacgcgct
    16861 ccgcgcgggc tgccacatcg tcacggacac gcagatggcg aaagcgggca tcaacaaaac
    16921 gatcctcggc cgtctcggcg gcgaggtgca ctgcttcatg tccgatgcgg acgtcgccgc
    16981 cgaggcgaag gagcgcggcg tcacgcgcgc gatcgtctcc atggagaagg cggcgcagct
    17041 gccggggaag tgcattttcg ccatcggcaa cgcgcccacg gcgctcatcg cgctgcacgg
    17101 cctcatcgaa gagggaaagc tcgaccccgc gctcatcatc ggcgtgccgg tcgggttcgt
    17161 gaacgtggtg gagagcaagg agctcttcct cgattccccg gtgccgcaca tcatcgcccg
    17221 cggccgcaag ggcggcagca acgtcgccgc cgccatctgc aacgcgctgc tctatcagat
    17281 cctgcggtaa catggaacag tacatcgaaa aggacggcaa gcgtctccgg ctcggatata
    17341 cgaccggctc gtgcgccgcg gcggcggcca aggcggcggc atggatgctg ctcaccggcc
    17401 ggaggaagga gaccatcacg ctcgatacgc cgaagggcat ccggctcgag ctcgccgtgc
    17461 gagagatcac gatgcgtgcg gacagcgtct cctgcgccat cgaaaaggac agcggggacg
    17521 accccgatgt gacgaaaggc acgctcattt ttgcctcggt ccgccggacg gacgagccgg
    17581 gcgtgcatat cgacggcggc gagggcgtcg gccgcgtgac gaagcgcggg ctcgaccagc
    17641 cggtcggaaa cgccgccatc aactccgtgc cgcggcagat gatccgcgag aacgtggagg
    17701 aggtcatggc gctcacggat tcctccggcg ggctggacgt ggtcatctcc gcgcccgagg
    17761 gcgagactct cgcgaaaaag acgttcaacc cccgtctcgg catcgtcggc gggatatcca
    17821 tcctcggaac gaccggcatc gtggagccga tgagcgaagc ggcgcttgtg gagaccatcc
    17881 gcgtggagct gcggcagcgc cgtgcgatgg ggaaggaata cgccctgctc acgcccggca
    17941 actacggctc ggacttcatc cggcagaatc tcgacgtcga tctgaatacc gcggtgcagg
    18001 tctccaactt tctcggcgac gcgctcgata tctgccgcgc gctcggcttt cgcggcgcgc
    18061 tgctcgtcgg gcatgttggc aagctcgtaa agaccgcggg cggcatgatg aacacccact
    18121 ccaaatacgg cgactgccgc atggagatcc tcgccgcgca cgccgcggcg gcgggcgtgg
    18181 acgcggagca catccgcgag atactcgact gcgccgcatg cgacgacgcg gtgcgcatcc
    18241 tgcgcgaatg cggtcgggac gccccggcgc tcgcgcgcgt cacggaaagg gcgctcttcc
    18301 atctttcgca ccgcgcggac ggcatggaga tcggcctttt aatgttttcc aaggaatacg
    18361 gcgttctggg gcagagcgaa aacgccccgg cgcttctcaa aaagatcatg gaggaatgaa
    18421 atcgtatggt acattttgtc ggagccggca gcggcgctgc cgatctcatc acggtgcggg
    18481 gagcgaagct gctcggcgag tgtgacgtgg tcatctacgc cggaagtctc gtgaaccccg
    18541 cgctgctcgc gtactgccgg aaggactgcc gcatttacaa cagcgccgaa atgacgctcg
    18601 agcaagtcat cgacgccatc cgaaaggccg aggccgcggg ggaaacgacc gtccgtctcc
    18661 acaccggcga ttcgagcatt ttcggcgcgg tgcgcgagca gttcgacgag ctcgacaagc
    18721 tcggcattgc ctacgacgta tgccccggcg tgagcgcgtt ctgcggcgcg gcggcctcgc
    18781 tcaaaacgga gtacacgctg cccgacgtga gccagaccgt catcatcacc cgcgcgccgg
    18841 gccggacgcc ggtgccggaa aagcagtcca tcgcggcgct cgctgcgcat caggcgacga
    18901 tggtgctctt cctcagcacg agcctcaccg aaaagctcca gcgcgagctg ctcgcgggcg
    18961 gctaccccga gagcacgccc gcggcggtcg tatacaaggc gacgtggccc gaggagaaga
    19021 tcttccgctg caccgtcggg acgctgcata agaccgttac ggaaaacggc cttacgaaaa
    19081 cgagcctcat catcgtgggc gagtgcatgg gggataagta tctccgctcg cttttgtacc
    19141 atcccggctt caccacggag ttccgggagg ccacggaatg aacgccgcgc tcttcgccta
    19201 cagccgccgc ggctgcgcca cggcgcgccg cgtcatggcg gcgctggcgg actgggacat
    19261 acagccgtat accatggagc ggtttgcgga ggacggcttc gcgccgctcg gccgtccggc
    19321 gagggagttt tacgggccga tcttcggggg cgtgcaggcc atgatcttta tttcgagcgt
    19381 cgggctcgcc gtgcgggagg tcgcgccgca tctcaaaagc aaggcgaccg accccgcggt
    19441 gctcgtgctc gacgagctgg ggcagtatgt gatccccgtg ctctcgggcc atatcggcgg
    19501 ggcgaacgag ctggccaaag cgcttgccgc ctcgctcggc gcgcaggcgg tcatcacaac
    19561 ggcgaccgac atcaacggcc gcttctccgt ggacgcgtgg gcagtgcaga atggctgcgc
    19621 catcgcgagc ctgccgctcg cgaaggccgt gtccgccgcc gtgctcgagg gggacgttcc
    19681 gtttctcagc gactttccgg tcgtgacgga tctgccgcgc gggctcacgc cgggcgattc
    19741 cggcgcgctc ggcgtataca tcggcgtcta tgaaaagacg ccgtttgcaa agacgctgcg
    19801 gctcgtcccg cgcgtcgtgc gtctcggcat cggctgccgg aagggaacgg acgaggaaac
    19861 gatccgcgca gcggtgaacg aagcgctcgc cgcgcacggc atcgaccggc gcgcggtgaa
    19921 gtgcgccgcg tccatcgatc tcaaggcgga ggaggcgggg cttctcgcct actgccgctc
    19981 ggaggggctg agcgcggagt tttacacggc ggacgagctg cgcgcggtgc gcggcgagtt
    20041 caccccttcg gaatttgtga aaagcatcac cggcgtggac aacgtgtgcg agagagccgc
    20101 gctccgaaac gccgaaacgt tattggtaaa aaagaccgcc cgcagcggcg tgaccgtggc
    20161 ggcggcggca gaacattggg aggtgcgctt tgcgtaagct ggctgttgtg ggcatcggcc
    20221 ccggaaacta tgaaaatatg acgatccgcg ccgaccgcgc gctgcgcgag tgcgacgtga
    20281 tcgtcgggta taccgtgtac gtcgatctcg tgaaggagcg ctacgcggat aaggaatttc
    20341 tcaccacgcc gatgctgcgc gaggtggagc gctgcgaaat ggcgctcgaa acggcgcgcg
    20401 gcggaaagac cgttgcaatg atctgctccg gcgacagcgg catttacggc atggcggcgc
    20461 tgctctatga gctgcgcggc gaggcagccg agcccgagat cgaggtcatc cccggcctca
    20521 ccgccgcgtg cagcggcggc gcgctgctcg gcgcgccgct cacgcacgac tttgccgtca
    20581 tcagtctgag caaccggctg acgccgtggg agaagatcga aaagcggctc gcgtgcgccg
    20641 ccgaggcgga tctcggcatc gtgctttata acccccgcag caagggccgc ccggacaatc
    20701 tccgcatggc gtgcgatatc ctgctgcggt atctgccgga ggaccggccc tgcggcgtgg
    20761 cgcattccat cgggcgcgag ggcgaggcgg gcgagtatat gacgctcggc gcgctgcgcg
    20821 atgccgatgt ggatatgttc agcaccgtgt tcatcggcaa cgccatgacg aaaatgatcg
    20881 gcggcgcgat ggtgacgccg cgcggctacc gccgcaaaga ggtgccggat gcctaagctc
    20941 tgcgtatttg ccggcacgac cgagggacgc gagatcgccg agtttctcgc cgagcagagc
    21001 gcggacgtga ccgtgtgcgt ggctacggaa tacggcgaaa cgctgctccc gcagggagag
    21061 gggctgcgca tttccgcgcg gcctctgccg cgcgaggaga tggcggagct cttccgccgc
    21121 gagcgcttcg atcttgtcat cgacgcgacg cacccgtacg ccaagcgcat caccgagagc
    21181 atcgccgcga catgcgccgg tacggacacg gagtatctcc ggctcctgcg gcccgacacg
    21241 gctgcgccgg aaaacgccgt ctttgtcgag gacgccgccg gagcggcgga gtatctcgac
    21301 cgcgtggacg ggaacatcct gcttacgacc ggcagccggg agctgcacgc ctttgcgtcc
    21361 gtccgcgatt tcgcttcgcg tgcttacgcc cgcgtgctgc cgatggagga ttcgctccgc
    21421 gcctgccgcg aggcgggtct gccgccggcg cacatcctcg cgatgcaggg gccgttttcc
    21481 agggaactca acgccgccat gctccggagc atcggcgcgg cgtatctcgt aacgaaggcc
    21541 tccggcagcg ccggaggatt tgaagaaaag atcgccgccg cggcggaaac gggcgcggtg
    21601 ccggtcatca tcggccgtcc gccgcagcgc gaggggctct ccgccgcgga aacggtggag
    21661 cttctctgcc gccggttcgg cttttccgca aagccggagg tcgccgtcgt ggggctcggc
    21721 cccggcagcc gcgccgcgct caccggcgag gcgcgcgaag cgctgtcccg cgccgggtgt
    21781 ctcgtcggcg cggcgcggat gatcgaagcc gccgccgcgc ccggacaggc ggcgcacgcc
    21841 gccatcgcgc cggaggatat ccgcgattac attctcgcgc acccggaata ccgctcgttc
    21901 gcggtcgtta tgtccggcga caccggcttt ttcagcggca cgaagaagct cctgccgctg
    21961 ctcgccggct gccgcgttac ggtgctgccg gggctgagct cgctctcctg tctctgcgcg
    22021 aggctcggca cgtcttacga ggatgtgctc cccgttacgc tccacggccg gagcgagccg
    22081 attttgctcc cgctcgcgca ggggcggcgg gtcttcgcgc tcgtcggcgg cgaaaacggc
    22141 atgggaaagc tctgccgnnn nnnnnnngac tgggtctgca cgccgcactg gatctccacg
    22201 tttcggtcgg cgagcatctg agctaccccg acgaggccat taccaccggc acggcggagg
    22261 agctttcgga aagacatttt gaaagtctcg ccgtggcgct cattgaatac ggcggcgaac
    22321 cgctgcccgt aacggcgggg ctgccggacg aggcgttttc gcgcgccgag agcgtgccga
    22381 tgacgaaaag cgagatccgc gccgtgtgcc tctcgaagct cgcgctgcgc gaagactccg
    22441 ttgtctggga cgtcggcgcg ggcacgggca gcgtcaccgt cgaagcggcg atggccgccc
    22501 gtcgggggcg cgtatacgcc gtggagcgca aagatgccgc ctttgatatc ctgcgcgaaa
    22561 atctccgcag gttccatacc gacaatgtga cgcccgtttc cggcaccgcg ccggacgcct
    22621 gcgccgatct ccccgcgccg acgcacgcct ttctcggcgg gacgaccggc catatgaagg
    22681 agatcatcgc gctgctgctg gagaaaaatc cgcgcgtgcg catcgtggcg acggcggtaa
    22741 cgctcgaatc cgttgcggag cttaccgagt gcgtgaaggc gtttccgttt cgcgaaacgg
    22801 aggcggtgtc tctcaccgcc gcgcgcagcc gcaaagccgg cgcctaccat ctgatgaccg
    22861 gccagaaccc ggtatacatt tttaccatgc aggggtgaag aaccgatgta ccatctcatc
    22921 gcgctgctga tcggcttttt tgtcgatctg ctgctcggcg acccgcacag catcccgcac
    22981 ccggtcgtgt ggatcgggaa gctgatctcg gcggcggaaa agctcgtacg gcgcctgttt
    23041 cccaaaaccg tccgcggcga gaacatcgcc ggaggcgtgc tctgggtcat tgtcgtgctc
    23101 gtctcgaccg ccgttccggc gctgcttctg tatgcggcct accgtctcca tccggcggcg
    23161 ggactcgtct tcgagagcat catgtgctgg cagatcctcg cgacccggtc gctgcgcgac
    23221 gagagcatga aggtctatgc cgcgctgaaa aagggtgtgc cggaggagta ccgccgcgcc
    23281 gtttcgatga tcgtcgggcg cgataccgcg gagctggacg atctggccgt aacgcgcgcc
    23341 gcggtggaga ccgtggcgga gaacgcttcc gacggcgtca tcgcgccaat gctctttctc
    23401 gcgctcggcg gcgctccgct cggctttttc tataaggccg ccaacaccat ggactccatg
    23461 ctcgggtata tcgagatgcc gtataagaac atcggccttg tcccggcaaa aatggacgac
    23521 gtgctgaact atatccccgc gcggctctcg gcgcttttga tgctcgcggc gggtgcgctg
    23581 ctcggtatgg acgcgaaaaa cggctggcgg atctggcggc gcgaccgccg gaaccatgcc
    23641 agccccaact ccgcgcagac ggagtccgtg tgcgcgggcc tgctcggcgt gcgtctcgcg
    23701 ggcgatgcgt attaccacgg cgagctccac aaaaagccgt acatcggcga cgcgctgcgc
    23761 gagatcgaat acgaggacat cccccgcgcg gggcggctgc tctacgcgac ggcggcgctc
    23821 tcgctcgttt tgttcggcgc ggtccgcttc cttctggtga tgtaaatgct gtacgatacg
    23881 aaaaaccccc acggcgggga tatttacgcc ggaaacgtcc ggctggactt ttccgccaat
    23941 acgaacccct tcggcacgcc ggagagcgtg ctcgcggcca tcacccgtgc gctgcccgag
    24001 gtgcaccgct accccgaccc ctactgccgc gcgctgcgcg ccgccatcgc cgcggcggag
    24061 ggcgtgccgg aggaatacat cctctgcggc agcggcgcgg cggagctcat atacgccttt
    24121 gccgacgccg tccggccgca gcgcgccgcc gagctcgcgc cgacgtttgc ggaatattcg
    24181 ctcgcgctcg cgcgcacggg ctgtgccgtg gcgcgctata cgctctcgga gaagaacgat
    24241 tttgcgccgg acggcggcat tctgaatttt ctgcgggaga cgaggccgga ggtgtttttc
    24301 ctctgcaacc cgaataaccc caccggacgg cttgccaatg gcgcgctgct taaagaaatt
    24361 ctcgcgctct gcgaaaagct cggaacttac cttttcgcgg acgagtgctt tcttgatctg
    24421 acggacggcg gcgagagcct gaagccgttc ctcacgcgct gcccgcggct ttttatcctc
    24481 aaggcgttca cgaaaagcta cggtatggcg ggcgtccggc tcggctacgg tctctgcgcg
    24541 gacgcagcgc tgctgcatgg tatgtccgag accgtgccgc cgtggaacgt atccacgctt
    24601 gcgcaggcgg cgggcgtcgc ggcgctgggg gagacggaat ttcttgaaaa gacccgcgcg
    24661 ctcgtgcgcg cggagcgcgc gtggctcgcg gaacagcttg ccgcgctcgg ctttcgcgtg
    24721 ataccctcgg acgcgaatta tcttctgttc cgcgctgcgc ccggactgga cgccgcgctg
    24781 cgggaacagg ggatcctcat acgaaactgc gaaaattacg cggggctttg ccccggctgg
    24841 taccgcatcg ccgtgcgcac gcgcgcggaa aacgaggcgc tcatccgcgc cgtacgggag
    24901 gtctgaccat ggcgaaaaat attatgatcc agggcacgat gtccaacgcg ggcaagagca
    24961 tcctctgcgc cggactgtgc cgcattttcc ggcaggacgg ctacagagtc gcgccgttca
    25021 aaagccagaa catggcgctc aactccttca tcaccgccgc gggcggcgag atggggcgcg
    25081 cgcaggtcgt gcaggcggag gccgcgggca tcgcgccgga cgtgcgcatg aaccccattc
    25141 ttctcaagcc gacgaccgac gtcggcagcc aggtgatcgt caacggcgtc gtgcagggca
    25201 acatgcacgc gatggagtat taccgccgca agcgcgagta tatccccgcg gtgatggaag
    25261 cgtataactc gctcgccgtg gagaacgata tcatcgtcat cgagggcgcg ggaagccctg
    25321 cggagatcaa tctcaagcag gaggacatcg tgaacatggg cctcgcgaag ctcgtggacg
    25381 cgccggtgct cctcgtcggc gacattgacc gcggcggcgt gttcgcgcag ctctacggca
    25441 ccgtggccct gctcgagccg gacgagcgag cgcgcatccg cggcaccatc gtcaacaaat
    25501 tccgcggaga taagaagatc ctggagccgg ggctcaaaac gctcgaggag ctgtgcggcg
    25561 tgccggtggc gggcgtcgtg ccgtatgtcc atctcgatat cgacgacgag gacagcctct
    25621 ccacgcgctt tgaccgcggc agcgagcgcc gcgcgatcga cattgccgtc gttcgtctgc
    25681 cgcgcatctc gaactttacg gacgtgagcc cgtttgagcg gtacgaaaac gtgtccgtgc
    25741 gctacattga gcgcgccgcg gagctcggag cgcccgacat gatcgtcctc cccggcacga
    25801 agagcaccat cgccgatctc aaatggctgc gcgagagcgg gctggaggcg gcgatcctga
    25861 aggccgcgga cggggggacg ctcgttttcg gcatctgcgg cggctaccag atgctcggcc
    25921 ggagcgtttc cgaccccgag caggtcgaag ccgcgggcat cacggacatc gcgggcatgg
    25981 gccttctcga tatggacacc gtttttcggg gcgaaaaggt gcagacgcag acgcgcggaa
    26041 cgttctccgg cgtggcggga gagcttgcct gcctgaacgg cctttcgtac gaaggatatg
    26101 aaatccatat gggacgcagc cgggaagcgc gcggcgttgt caccggcggc gggaacgtgt
    26161 acggcagcta tatccacggc attttcgacg cccccggcgt cgccgacgcc gtgctcggcg
    26221 cgctctgccg caaaaagggg ctgagcccct ctattctcgg cgctttcgac cccgtggcct
    26281 accgcgagga gcagtacgac aagctcgcgc aggtcgtgcg cgaggggctg gacatggagc
    26341 tcgtgtaccg catcctcgac cggagggact gagcaccttt ccccaggtcg ttttttcgga
    26401 aattctgcgg gaacatatat tgcaaagcta tcatgtttat gctatatttg ctatacatgg
    26461 atgaatctcc cgtaaaattg aatagaagca tccgaatgaa ggaaccgtgt gagaatcacg
    26521 gactgtaacg gcaactgtaa cgcggagcga ccccgttatg ccactgggaa accg
//
LOCUS       DAWAAE010000012        26098 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_808_length_26098_cov_3.595170, whole genome shotgun sequence.
ACCESSION   DAWAAE010000012 DAWAAE010000000
VERSION     DAWAAE010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 26098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26098
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_808_length_26098_cov_3.595170"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15071..15080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20348..20357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21470..21511
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4815 a         8395 c         7999 g         4827 t
ORIGIN      
        1 cagcaccgcg gcgctcgcgc acaacgtttc ggttttgcgc ggcgcgctgc cgccggggtg
       61 cgagctgatg gcggtcgtaa aggcggacgc ctacggccac ggaatgtccg ccgtcgtgcc
      121 gcagctcctg tcgctcggtg tgcgctcgtt cgccgcggca acgctcgacg agggttcgca
      181 gctccgcgcg ctcggcgcag aggggacggt actcattctc ggctgcacat cgccgcaacg
      241 cgccggggag cttgcccgtc tcggtcttac gcagaccgtg gccgaccttg cgcacgccga
      301 agcgctgaat gcgcaaggcg tgccgctgcg cgtacatttg aagatcgaca ccgggatgca
      361 ccgcctcggc atcgcgtggg acgcgccgga ggcggcgagc gcggtgtttc gcatggaaaa
      421 tctcacggtg gacgggatat attcgcatct ctgctgcgcc gacagtctcg cgccgggcga
      481 tgtttcgttc acacgcgagc agatccgccg gttttatgcg ctgacggcgg cgcttgccgc
      541 gcagggcatt tccctgccga agctgcatct gcaaagcagc tatggtctcc tgaactatcc
      601 ggagctgcgc tgcgattatg cgcgcgtcgg ccttgcgctc tatggcgcgg cggagggaac
      661 cgcagcgcgc tcgctcggtc tgcgtcccgt tctctcgctc cgggcgcgcg tcgcctccgt
      721 tcgagatgtg gcgaagggcg agagcgtcgg gtatgaccgc gcgttcactg cgcgtcgaaa
      781 gagccgcatc gccgtgctgc ccatcggcta cgccgacggc tatccccgtg cgctctccaa
      841 taccgggctc gtccgtatcc acggatgtct cgcgccggtc gcaggacgga tatgcatgga
      901 tcagctcgcc gtggacgtaa cggacgtgcc gggcgtcgtc gacggggaca tcgccgagct
      961 tatcggcgat gcttcgccgc tgcgcgccgg ggaagtcgcc gcgcgctgcg gcacgatccc
     1021 caacgaactt ctgagccgcc tcggcgcgag gctgccggtg gtaataaaat gatacaaaaa
     1081 cacccaagac cgcagtcttg ggtgtttttt agatgatggt gtaaaagttt aatccgccag
     1141 ccgctccatc tcgtcgatga gctcgccgaa gagcgcgagc gcgtcgttca cgggcttcgg
     1201 cgtgctcata tcgacgcccg cgatcttgag aaggctgatc gggtcggcgc tgccgccgga
     1261 ggagaggaac ttcttatagt cggcgacagc cggttcaccc tcggcgagaa tgcggtttgc
     1321 gatcgccacg gcagcggaga agccggtggc gtactggaac acatagtagt tatagaagaa
     1381 gtgcgggatg cgcgcccact cgagcgcgat gccgtcgtcg gagaccatgt cggggccgaa
     1441 gtacagcttg ttgagcgcaa gatacttttc actcagcgcg tccgccgtga gagcctcgcc
     1501 cgcgccgttc atctcgccca tgatcttctc aaactcggcg aacatcgtct ggcggtatac
     1561 cgtgcccttg aactggtcaa ggaagtgatt gatgagatag gcgcgctgct tcttgtcggt
     1621 cgtcttgccg agcagatggc gcatgagcag cacctcgttg caggtggagg cgacctcagc
     1681 cacgaagatg acgtacgcgg cggtattcac cggctggtac ttgcagctat ggtagctgtg
     1741 cagcgcgtgg cccatttcgt gggcgagcgt gaacatgctg tcgagattgt ccttgtggtt
     1801 gagcaggaca taggggtgcg ggtagctgtt gccggaggag tacgcgccgc cgcgcttgcc
     1861 ggtgttttcg taaacgtcga tccagcggtt ggcgaagccc tccttcagca ggtcggtata
     1921 atcctcgccg agcaccgcga gcgcttcgag caccgtggcc ttggcttcct cgtaggtgat
     1981 agcgctgtcg gcatccgcca cgatgggggt gtacacgtcg tacatgtgca gctcgtccac
     2041 gccgagcagc ttcttgcgca gcgcaacata gcggtacatc ttgtccatgt tcgcgtggac
     2101 ggcctcgatg agattgtcgt aaacctcttc cggcacctcc gtcacgtcga gcgcagcggc
     2161 gcgggtggaa ggataatgcc gtgcgtccgc gaagaacttg agcgagcgga actgcccgtc
     2221 gagcgacgcg gcgacggtat ttttgaattc gccgaaacgg ttatagtaag cttcaaaggc
     2281 gtttttgcgc agcacgcggt cgctgcccat gagcaggggg acgaacgtcc cgctcgtgag
     2341 aacgcgctcg tttccttcgc cgtcggtcac ggtcgggaac gtgagatcgg cgtcacggaa
     2401 gacgctggcg atattttccg ggccgttcgc catctctccg gcggcggaga ggagcttttc
     2461 ctcctccggg gagaggatgt gcgcacggcg gcggcgaatt ttgtaaatat tgcggcggta
     2521 ggtctccagc tccggctgcg cggcatagaa gagattcacc gtgtcctcgt cgagcgccat
     2581 gatctccggc gtttcaaacg cgctcgccgc gtcgatggcg acgagcgtcg caagcgcctt
     2641 ggcgcgcatg ccctgataaa cgctgttgcc ggtgtcctcg tcgcccttgc agttcgcgta
     2701 gccgtagagc ttttcaagcc gcacggagag cgcgtcgttc aggcggaacc actccaaaag
     2761 cgtctccgcg ctctcgccga gcctgccgcg gaacgcttcg atttgctccg gcacggtctt
     2821 gaacgcttca tactccgcct gccaggcctc gtcgctttca aatacgtcct tgagattcca
     2881 ggtcgattcc tccggcactt cgctgcgcgc cgggatgggt ctgtctgcca ttgggtatat
     2941 cctcctgcaa agttaaaatt tctttttcgg gagcttgtgc tccttgccgt cctcaccgat
     3001 gatgtagacg ttttcgagcg tttcgagcgt cccgcgccgc cagtcggcca gatattcctc
     3061 gcgcagggcg aggcgctccg ccgtttcctc gggcgtgagc gcgcgttcgc gcgaaatgcg
     3121 cgtcagttcg ctgatgcggt ttttcttttc ttcggtcatg gtcacattgt ccccaattcg
     3181 tccagattct tttcaaacgc gccgaggcag tgctccataa accagtccgc ctccgggaga
     3241 tgcatctcct caacggcgcg gcgcgtctgc tcctcggcgg agagaaattc gcggttgccc
     3301 gccttgcgct cttcgatgca ctggcgcagc gccgagagct tgtcggctgc cttgacgagc
     3361 ttttcctcgt cctccgatgc cgtaaacgcg ctctcgtatg ccccgcgcag ctcctccggc
     3421 agcatggcga gaagccgctc gtccgcggcg tgctcgacct ttttataggc gtcgcggagc
     3481 gtgcggtcgt ggtacttgat cggcgtcgga agatcgccgg tcagaatttc gctcgcgtcg
     3541 tggtagaccg caagcaccgc gagacgctcg gcgtcgcatt cctgcccgaa gatctcgcgc
     3601 cggatgatgc cgagcgcgtg ggcgtacacc gccgtttcat ggctgtgctc ctgcacgttt
     3661 tccgggatgc tgctgcgcat gagcccccag cgggaaatat accgcatgcg cgacaaaagg
     3721 gcataaaacc ccgtttcctt caccatttgt tgctcacctt ctccgcaaag ccgggaaaat
     3781 ccgcaaactc caccacggtg ccgttatcga gaacggccct gccggtaaac cttccgaaca
     3841 cctggtgctg gtcggacttg atgagcttta cgtccgtgca gctcgcgcgg tcgagcaccg
     3901 gcacgaaatc ggcctcgaag tgcccgtcgt cggacgtgaa cttccatggc tcggtatagc
     3961 gctcttttcc ctcggcttcg ccggggatct caaatttgat gccgccgagc ttgtgcgcgg
     4021 taccggcgtg gaagagcatg ttttcgctcg ccgcgctcgt atcgccgaag ccgtagccga
     4081 gattccagcc gaacggcgtc ccgctcggca gcacatatgc cgcgctgctc cagtaccacg
     4141 tattttcata cgtccacacg ccgcggcccc agtcgagcac ggcgaagctg tcgctcggcg
     4201 caaaggtata cgtcttgccg ttataattaa catacccgct cgcgcgaaag cccacgatct
     4261 tctggttata gtagaaatgg ccgggcttat caaagggcgt ggcgatgacg agcgaatcct
     4321 ccgggcattc ggtgagctcg acgcgcgcgg agagggcgct ctcgccgaac ttgttcatat
     4381 acgcgatgag gacgcgcttc tccccctcgt ggatgaagtg gataccgtag ccgcgcgcgg
     4441 cggagtgcac atcgccggtc acgctcgtct ccggcaggtg ctttttgccg agcggcagga
     4501 aggacatcgg gcttttcgtg atctcccagc cctcttcgag atcgagcaga ctcacggagt
     4561 ccagtcccat atagccgtta tccgccacgg tgagcgcgag agcgaatttg ccgttgttga
     4621 cgagataata atcccattcc ttgatgcgca ggggcgagac cttcacgtcc gcgcggcggt
     4681 attcctgcac gagcgcgcgg gaaaagccgg ggcgggcgag atcgcccttt tcgttgagca
     4741 gcgcgccggg ggtcgtgatc tcggtctgtc ggtccattca gatgccctcc ttacgctggg
     4801 cgcggatgtc ccggcccaga aattccagcc attcgtattc tccggcgagc cgcgaggcga
     4861 gcgccgcgcc gtagcgcgtt tcgatgccgg agcggtcgag attcgtggag acgatcgtct
     4921 ttttccctgc gcgcaggcgg ccgtcgagca gggcgtaaag cgcgctctgc gtgaacgccg
     4981 tcagcatttc ggtgccgaga tcgtcgagaa tgagaagatc gcagctcata agctggcgca
     5041 cgcgcgaggc ggcgtccgat acctcgtccg tatcgcgcga gaatttctcg cgctcgaacg
     5101 aggcgagaag cccgctcgcc gtatcgtacg ccacgccgca gccctttgcg gccacgacgc
     5161 gcgcgatggc ggcggagagg aacgttttcc ccagccccgg ctcgccggtg aacagcagat
     5221 tcggcgactg cgcgccgaag ctctcggcat aggcgcggca taggcgcagc acgcgctcca
     5281 tctgcgcgcg gggcgatacg cccgaggcag gggcaacggg gctgtagtaa tccagccgga
     5341 agttttcaaa cgtctcgtcc ccctgcttca gaaggggcgc gagctctctg gtctgctcgg
     5401 cgcggtagag cttttggacg cactcgcaga tcttcccgtc cgtccagccg gtatcgcggc
     5461 agcgcgggca ggaatagatg gggtcgagat aatccttcgg gtatccgttc tgcacgagaa
     5521 gcgccgaacg tttttcctgc aaagcgaggc tttccttctc gagctcctgc gcggtgcggg
     5581 cgctctgccg catggcgatg cgcaccatct cgctcagatt ggcgcagagg gccgtgtcga
     5641 tgcgtccgat ctccgggatc ttcgcggcga tctcgcggcg gcggcgatcc tcctctgcgc
     5701 ggttttccgc gcgcagcgtc tcctgtttgg cgcgggcgcg cgccagaagc cgtccttcca
     5761 tatcctctta ccccccgatg ttcttcagta tgccctcgag ccgggcgaga tcgtccccgc
     5821 cggtgcgccg cggcgcgctt tccgcagcgg gcttggcgcg cgacgatgcg cggctgtcgc
     5881 ccttctcgat ctgctcggcg gtgaagagct ttttctcgta ccagctcttc acgatcttgt
     5941 ccatatacgc ccaggcgagc ttgcccgtgc cgacgacggt gcggtcgtag gcgatggcga
     6001 gcgcctccgc gccgtagccc atcgaaagcc agctttcgat gtacttgcgc tccgtgggcg
     6061 tcaggtcgcg tcccgtgagc gagagcgcgt ggcggacctt ttccatctcc tcgtcgcgcc
     6121 gggcgagcgc ggcgagatat tcctccgcct gcggcgcggt gaggatctcg cggttggccc
     6181 agtagaacgc ttccttctcg atggcgcgca tcgtcgggag gcgtccttcc ccgtagcggc
     6241 ggcggagctt gtccgcgcag tggtggatga gcagcaatat cgtctccgcc gtcatgccga
     6301 gacggtcgta aatgccgaaa agcgtgttga gatcggcgct cgagagcgtg tggccgagca
     6361 cgcgctggca ctcgccgagc agcgcgcgga acgccccgtc atcggcgctg cggcgcaata
     6421 tgtcctccgt gcggtatacc ggcagctcgt ccggtcccgg cagaggcacg tccccggcgg
     6481 tgagaaggcc cgccccggag agccggcgcg ccgccgcggt gatctgctct cccgtgcggg
     6541 agagagcgcg cgccgccgcg gcggtatcga gcacgccgcc ggagcgcaga atataaagat
     6601 acagcagcgc gcactccccg tcgccgagcg cgaggagctt ttcggcgtcc tccgccggga
     6661 gagagagcgt gttcgcgccc tggatacgat agcgttttcc gtccatgttc gtccttttgg
     6721 ggtatagtcc cccataatct atgtgtatcg gatattatat cataatgttt ctacttgaag
     6781 caagggtgtt cgttgtgtta taatcttatt ttgaagaagt accgttttcc cggtaccgtg
     6841 tttacgagga agaaattatg gtcgaactgc tcgctcccgc cgggtcgccg gaggccgtca
     6901 ccgccgccgt gcaaagcggc gcgaacgccg tatatctgag ctttgaggat ctgactggct
     6961 gccgccggac ggagaacatt ccgaacagcg aatttgagtc ggtcgtgcgc tactgccgca
     7021 tccgcggctg ccgcgcatat ctcgcgctca acatccccgt ccgcgaggac gagctgaaca
     7081 aagccgcggg ccttgccctg cgcgcgcagc gctgcggcgt ggacgcgatc atcgtccgcg
     7141 atctcggcct tttccgcatt ctccgctcgc ttctgccgga gatgccgctt tttgccgacg
     7201 cgcatctcgg cttctatacg ccggagagcg cggccatcgc gcagcggctc ggcttccggc
     7261 gcgttttcct gccccccgat ctcccgcttg aggatatccg ccgcatggcg cagtgcccga
     7321 tcgagacggc cgtgtgggtg cagacgccgc tttgcgccgc cgcgtgcggc acgtgccgca
     7381 tgagcgcctt cgccgggcgc gagagcgccg agcgcggcct ttgcagcgag ctctgccgcg
     7441 agcagtacac gctcggcggc cgctgggaca caacgcctct gagctggaag gaccgctgca
     7501 tgcttcccgc gtgccgggat ctcatcgacg cgggcgtagc ctgtctcgcc atcggcagcc
     7561 gcgagcgccg gagcgagtat gtctccgcgt tcacggacgt gtgggcgacc gccatccgcg
     7621 aaagccagct gcccgccgag ccggagctgg agcgcttgga gcgcgtgttc ctcccgtggg
     7681 gcgcggcgaa aaaagatctt tacgaaaccg ccgaagcgcc ggagaagatc cccggcgaga
     7741 ccgaggccgt ctgcgccgag cagcgccgga actatacgtc cggcgaggcg cgccgcgtcg
     7801 gcgtgagctt tgcggtcgcg gcgaaggacg cgcactcgag cgtcattctc ggcgcgcagg
     7861 acacggacaa gaccatcgcc gcgatcgaag gccccgtgcc ggacgatacc ggcgacgtgg
     7921 agctcaccga ggacggtctg cgcgaggcga tgtaccgcac ggccggaacg ccgttccgct
     7981 gcactgaggt gcgcgtgcag tctcccgcgg gagaaaagct ccggatatcc ggcatcgaac
     8041 tggacgaggc ccgccgccgt cttttgtata agctctccga ggagcgcgcc aagctcccgg
     8101 accgcaaaga gggcgacttt cccgagacgc cgcgcatcgc ccccgcgccg cacctgcccg
     8161 cgatcaactt ttcgttccag accgccgcgc agatgacgcc ggagctggcc gcgctctgtc
     8221 cggagtgcgt atacgtcccg ctcgagctgc tcgcggaaaa tccgtcggtc gctgatgcgt
     8281 tccgcgaaaa cggcgcggag atcgtcgccg cgctcccctc cgttgcgttc ggcgagaagg
     8341 aagagagcga gctgcgcgcg cttcttgcaa aggtgcacga gagcggcgtc acgcaggcgc
     8401 tcaccggaaa tctcggtctc gcgatgatgg cgggacagga gggcttccgc atccgcggcg
     8461 actttgatct cgccgtgatg aacagctata ccctttccgg catggcgtcg gcgggttttc
     8521 tctcggcgat gctctcgccg gagctgacgt ttgccgacat ccgcgagatg cccaagtgca
     8581 tggacgccga gctcgccata tacggccgtc tgcccgtcat ggtgacgcag acctgccttt
     8641 tgaaggcaag tgccggacgg tgcacctgca ccacgcccgg ccagatggcc gacacgcacg
     8701 gcggcgtatg gccggtgacg aagcatttcg gctgccgcaa caccgtttgg gcggcgcgca
     8761 agctctggct cggcgacgtg acgcaggagt ggatcgatct cggtctctgg gcggtgcggc
     8821 tgtgcttctc cacggagagc gcgcgcgaat gtctggaagt tgccgagagc tatatccagg
     8881 gcacgcatta taagcccaac ggcataacga gaggactgta ttaccgtggc gtactgtaat
     8941 aaacggctga tcctcgcctc tggctcgccc cggcggcggg agctgcttga taaattcggc
     9001 ataaactatg agatcctccc cgcacagggc gaggagagcg cgccgccgga gctcacgccc
     9061 ggcgagcggg taaaggcgct cgccgcgcag aaggcggagg aggtggcgca gcgcctaaac
     9121 gatcccgccg ccgtgatcct cgccgccgac acgctcgtgg agctggacgg tgaggtgctc
     9181 ggaaagcccg gctcggcgga gcgggcgcag gccatgctgc gcgccctctc cgggcgggag
     9241 caccgcgtat ggagcggcgt gtgcatccgc gagggggaaa aagttcttgc ggagagcgag
     9301 tgtacctccg tccgtttccg cgctctttcc gacgcggaga tccatgccta tattgccacc
     9361 ggcgagccgc tcgacaaggc gggcgcttac ggctatcagg ggctcgcgag tctctttgtg
     9421 gagcgcatcg agggcgattt cttcaacgtg atggggcttc cggtctgccg tatggggcag
     9481 atgctccgcg aatttgacat ttctcttctc tgattttttt actcgcgccc gggacgttcc
     9541 cgggagaaca gggggtcaag ccttgaaaac ttatctgaag caatacggcg tgcgcatggc
     9601 gatcatcatc gttgtggcgg cgcttctcat aggcctcgcc gcgcgcccga aggcggacgg
     9661 cagctccggc gcggtgccgg aggcggccaa cgccctgcgc atccccattc agagcgccgt
     9721 gggctctgtc gtggagtgga tgcgcggcat ttacggctac atctaccgct acgataagct
     9781 gctcgcggaa aacgaatcgc tccgccgcca gcttgccgag gcgcaggagc aggtgcgctc
     9841 gagccaggac gcgctcgagg agaacacccg tctgcgcacg atgctcgggt atttggaaaa
     9901 gaacacgacg ttcaagaccg aagccgccat gatcacctcg tgggacgcct ccaactggac
     9961 gagcgccttt acgatctcca agggtacgga caacggcatt gagcagggcg actgtgtcat
    10021 caccgagtcc ggcatgctcg tcgggcaggt gtatgaggtc ggcaagaact atgcctccgt
    10081 gcgcacgacg atcgacgtga acatggacgc gggcgtgctc gtgggcgaat cgtccatcgc
    10141 cgcgatgatc gtcggcgact atgcgctcat gaaggagggc tgctgcaagc tcagctactt
    10201 caccgacgat gtgacgctct tcaccggcga cagcgtcgtc acctccggcc gcggcggcgc
    10261 gttcccgtcc ggcatcatcg tcggcaccgt cacggagctg cgcagcgagg cgggcggcca
    10321 gtcgtattac gcggtcgtca agccctcggt cgatctttcc gtgctgtcgc aggtcttcat
    10381 catcaaggat tttgaggtca tcgagtgaaa cgaaacaaga tcaatttcca gctttccgat
    10441 atgaagctgg acgagctctg gctgaagacc cggcgcggcg ttttgtatgc gctgtatctc
    10501 atcggcgcgc tgctcgtgca gaacgtgata ttgagccacg ttgcgctctt cggcgtgcgg
    10561 gccatgtttc tgcccgcgct cgtcgtggcg gcggcgctct ttgaaggcgg ctggggcggc
    10621 gggctgttcg gtctggccgc gggcattctc tccgatctct tttttggctc gcagagtgtt
    10681 ctcttcaccg ttctcttccc cgtgatgggc ttcatcgcgg gctttctcgg cgatttttat
    10741 ctcaacaagc gccttttcac ttactgcatc ctgggcgcgg cggcgctctt tctcgccgcg
    10801 ttcgcgcaga tgttcagtct gctcttctac gagggacaga gcagcgccgc gctctgggta
    10861 acggcgcttc tgcaaacgct ctggagtctg ccgttcctct ttccctcgta ctatctctgc
    10921 aaaaatttcc cgtggcgctc gtcgcgggac gtgcctacgc cgtataaata atttcacccc
    10981 ctccgcattt tgaaaggaac tttccatgca gaaaatggta aaacccatcc ggctcgttgt
    11041 gctcgccgtc gtctttgtgc tgctgctcgg cgtttacttc gtcgcgcttt ataagctgca
    11101 gatcatcggc gggcgcgaat actacgccgt aggggaaacg gagacgaaag aagacatcac
    11161 catcgccgcg gcgcgaggga acattctcga ccgctacggg cgtctgctcg tgtccaactc
    11221 gctctgcaac aacctgtcca tcaacgtaac ggagctcttc agccaggacg acccgaacgc
    11281 tgtcattctt gagctgtgca gcgccgtgga gcgcttcggc gacacctata ccgacacgct
    11341 gcccatcacg aaggccccgc ccttcgagtt caccgagatg gacacgctgc agaagagcat
    11401 gctcgacgcc tggctcaagg cgaacgacat gccgctcgac tccagcgccg tggagatcct
    11461 cgcggcgatg cgcacgcgct acagcgtcgg cagcgagtat aacgccgagg acgcccggcg
    11521 catcgtcggc gtccggtacg agatcaacga ccgcttcaac atcaacacca cgccctacgt
    11581 tttcgccgag gacgtttcaa tgccgctgct cacctatctc atggagcagg acatccccgg
    11641 ctttgagctt tcctcgtcct atgtgcgcga atacaaaacg cagtacgccg cccacctgct
    11701 cggctacacc ggcaagctga cggcggagga atgggcgacc tacgccggca aggactactc
    11761 gctcgacgcc tatgtcggca aggacggcgt ggaatacgcc tttgaggaat atctccacgg
    11821 cgtggacggc gaggcggagg tctactccaa tctcgacggc gtcacgctcc gcaccaagta
    11881 caccaaggag ccgcagcccg gcgggcatgt ctatctgacg ctggatatcg gcctgcagga
    11941 ggtgtgcgag gaggcgctcg ccgcgcacat cgagtcgatc aacgaagatc gcgcccgcat
    12001 cattgcggaa gccgaaatgt acggcaccga gagcaccgaa atgatctccg gcggcgcgct
    12061 gtgcgtcgtg aaggtcgata ccggcgagcc gctcgcgctc gtgagctatc cgacctacga
    12121 cgccgccacg atgctggaga attacgaaac gctcgtcgcg gacgagaacg ccccgctcta
    12181 taaccgcgcg ctcatgggcg tttacgcccc cggctccacg ttcaagccct gcaccgctac
    12241 ggcgctgctc gccgagggca tcatttcccc gacgcgcacg ctgaagaccg aaggtcagtt
    12301 caccaagtac atcgacgacg gctacgcccc cgagtgctgg atctactcga cctacaaata
    12361 cacccacggc gagatcaacg tcgtgcaggc gatcacctac tcctgcaact acttcttcta
    12421 ctatttcggc gacgagctcg gcatcgacaa gatggcgtac tacgccaagc tctacggtct
    12481 cggcgagcac accggcatcg agctgcccga ggtcaccggc cagatgtcca cccagagctt
    12541 caaggagcag tacaccggta tctcctggtt ccagggcgat acgctgcaga gcgccatcgg
    12601 ccagtcgttc accgaattca cgccgctgca gatggcgggg tattgcgcag ccatcgccaa
    12661 cggcggcacg cgccacagtg cctccattct caaggaagtg cgcagctacg attactccaa
    12721 aacgctcttc cagcgtgaaa cggaagtgct cagcaagctt gatatcgatc ccgagatctt
    12781 cggctacatc cagtacggca tgtacggtgt gctctatgac gccgcgagca cgctcaagga
    12841 gcattggctc gacagcagca tcgtcgccgc cgccaagacc ggtacggcgc agcgcggcga
    12901 aggtctcgtc aacaacgcca tgttcattct ctatgccccc tatggcgata accccgagat
    12961 cgccatcgcg atcgctgtgg aaaaaggcgg cgcgggcgcc acgctctcgc cggtcgcgcg
    13021 aaagatcgtg gattattact tcacgttcca gtccagcgcc aacactgtgg aaaatgcgta
    13081 ttctctgctg aaataatccg aaattttcgt tgcaaatagc gctttttgcg gttataattt
    13141 aaaaggtttt atctgcccaa atcatcaaaa atatacggag gtgccaaagc atgaatatcg
    13201 ctcttctcgc ccatgacgga aaaaaggagc ttatggtcca gttctgcatt gcctactgcg
    13261 gcattctctc cggtcacgac atctgcgcca cgaacaccac gggcaagctc gtttccgagg
    13321 cgacggggct gccggtcacg ctgtatctgc cctgcacgca gggcggcgcg cagcagatcg
    13381 gcgcgcgcat cgcctacaac gagatcgacc tcgtgctctt cttctgcgac cccaaggcca
    13441 acacgaagga cgtggacgcc atcgcccgcc tgtgcgacca gcacagcatc cccttcgctt
    13501 ccaacgtcgc caccgccgag gtgctcgtgc agggtctccg ccggggcgat ctggactggc
    13561 gcgatatcgt caacccgaag aaaaaataaa aacaccctac aacttttccg aggaatggat
    13621 agccggcagc ggccagctat ccattctcta ttcaaaggag gattccccca tggcccagat
    13681 ccgaccgcac acgctctccg gcttcatgga gcttctgccg gaagaccaga agaaaatgca
    13741 gcgcgtcatg gcgacgctgc gcgagagcta tgctctctac ggcttcacgc cgctcgacac
    13801 gcccgtcatc gagagcgccg acgtgcttct cgccaagggc ggcggcgaga ccgaaaaaca
    13861 gatctaccgc ttcacgaagg gcgacagcga tcttgccctc cgctttgatc tcaccgtgcc
    13921 gcttgcaaag tacgtcgcgc tgcacgccaa cgagctaaca ttccccttcc gccgctacca
    13981 gatcggcaag gtctaccgcg gcgagcgcgc ccagcgcggc cgcttccgcg agttttatca
    14041 ggcggatatc gacatcatcg gcgacggcaa gctcgacatt atgaacgagg ccgagatccc
    14101 cgccgtcatt taccgcacgt tcaacgcgct cggactcaaa aacttccgca tccgcgtgaa
    14161 caaccgcaag gtcctgaacg gctttttcgc tctgctcggg ctgacagaga aatccggcga
    14221 cgtgatgcgc acgatcgata agctcgacaa gatcggcccc gacaaggtcc gcgccgtgct
    14281 cacggacgag ttcgccgtcg agccggagac cgcggataaa gtcctcgagt tcatctccat
    14341 gcccggtacg agcgcggata agctcgcctt cctgcgccgg tacgagggga agaacgagac
    14401 gttcgatctc ggtctgcacg agctcgccac cgtcgtggag tacgtcggct cgttcggcgt
    14461 tccggcggag aattttgaga tcgatctcac gatcgcgcgc gggctcgact actacaccgg
    14521 cacggtgtac gagaccgtga tgaccgacca tccggagatc ggctcggtct gctccggcgg
    14581 acggtacgac aatctcgccg ggtactacac cgaccgtacg ctccccggcg tcggcatctc
    14641 catcggcgtg acgcgccttt tctatgtgct gcaggagcag gacatgctct ccaaggacat
    14701 cctcaccgcc cccgccgagg ccgtcgttat cccgatggac acagactgca tggcgtttgc
    14761 cgtggagacc gcgacggcgc tgcgcgccga gggcgtcaaa acgcagatct attttgaaaa
    14821 ccgcaagttc aagcagaaga tcggctttgc ggataaatcc ggcattccgt tcgcgctcat
    14881 catcggcggc gacgaggccg ccgcgggcgt cgtatcgctc aaggatatgg ccgcgggcac
    14941 gcaggaaaag ctcaccccgg cggaagccgc ggcgaagatc aacgccgcgc tcgcgccgcg
    15001 ccgcagggcg caggccatcc gcggataaac gttcccgatt cgatttttaa gaaaggcggc
    15061 ggggatatgg nnnnnnnnnn ggatatggtt gcgaaaatac gctcgctcgg cttgcagggc
    15121 atcggcggct atgaagtctc ggcggagatc tttctctccg gcgggctgcc gcagtttgac
    15181 cttgtcggcc tgcccgacgc cgccgtgaag gaggcgcgcg agcgcgtgcg cgcctcggtg
    15241 aagaattgcg gtcttgactt tcccgtcagc cgcgtcaccg tgaatctcgc gccggcctac
    15301 cggaaaaagg ccggcaccgt atacgatctc ccgatcctgc tcggcatcct catcgcctcc
    15361 ggacaggcca ggcctctgcc gccggacgcc gccgtgatcg gcgagctctc cctctcgggc
    15421 gaggtgcgtc cggtgcgcgg cgcgctcccg atggcgctcg ccgcggaaaa ggcgggcatc
    15481 cgggagctgt acgtccccgc gggcaacgcg cgcgaggcgg cgtacgccga ccatctcacc
    15541 gtctatcccg tacataccgt cccggagctt ttggcgcatc tcgcgggcga gaagaagatc
    15601 acccccgccg agccgcccat gccgagcgag gaagagcttc tcttccccga ctatgccgag
    15661 gtcaaggggc aggaaaacgt caagcgcgcg ctcgaggtcg ccgccgcagg cgggcacaat
    15721 gtgctcatgg ttgggccgcc gggttcgggc aaatcgatgc tcgccaagcg tctccccggc
    15781 atactgccgg acatgagccg cgaggagacg atggagtcca ccggtatctg gagcgtctgc
    15841 ggtctgacgg acgaaaagcg ccccatactg cgccagcgcc ccttccgcgc gccgcaccac
    15901 acgctctccg ccgccgcgat ggcgggcggg gcgcagctcc agccgggcga ggtatcgctc
    15961 gcgcacaacg gcgtgctgtt tctcgacgag ctgcccgaat accaccggga cgtgctcgag
    16021 gtcatgcgtc agccgctcga ggacggcgtc gtgaccgttt cgcgcgccgc cggaaccgcg
    16081 gtatacccca gccggttcat gctcgtgtgc gcgatgaacc cctgcaaatg cggctggtac
    16141 ggacagccgg gcggccgctg ccggtgcagc gaaaagtccg tccgcgccta ccacgccaag
    16201 ctctccggcc cgctcatgga ccggatcgac atcattgtag aagtccccgc gctcgcctac
    16261 gatgagctgc gccgccgtcc ggacggcgag agctccgaaa ccatccggca gcgcgtcaac
    16321 cgcgcgcggg aggtgcagcg aaagcgcttt tcctccggca cggtgtccaa cgccaatatg
    16381 tccccgcgcg atatggagcg gtactgctct ctcacgcccg agggcgacgc gctcatgaag
    16441 gacgccttcc acgcgctcgg tctcacggcg cgaagctacg accgcatact ccgcgtcgcg
    16501 cgcacgatcg ccgatcttgc cggggcaaag gacatcgcgc ccgagcacat cgccgaggcc
    16561 gtgcagtacc gtacctacga ttttctcatc tcttcctccc cggaggtcac gctatgaacc
    16621 gattcgatgc cgtcctgttc gatctggacg gaacgctctt atatacgctt gccgatattg
    16681 ccgcggccgt caacggcgcg ctgcgcgaaa tgcgccgccc ggagcggacg gtcacggaga
    16741 tccgctccat ggtcggcaac ggcgcgaaaa tgctcatcac ccgcgcgctc ggtccggggc
    16801 aggaggcgct cgttgacgat acgctcgctg tctacgccag gctctacgcc gcccacgcgc
    16861 aggagcatgt gacgccctac gcgggcatcc cggagcttct ggacgcgctg catgaacgcg
    16921 gcatccgcac cgcgtgcgtc acgaacaagg accacaccga cgccgcgccg atgctgcaaa
    16981 agtttttcgg caaaagaatt tcccacgttg aaggccggaa gccgggccgc cccacaaagc
    17041 ccgcgcccga cgcggtgtac gatgcgctca aagcgctcgg cgccgatccg tcccgcgcgc
    17101 tctatgtcgg cgattccggc gtggacagcg aaacggcgaa aaatgcctcg ctcccctgcg
    17161 tcctctgcgg ttggggatac tgggaccgcg acagattaga gaactgcgcc gcgctgggga
    17221 tcataagtgc cccggcggag ctattacaat atgtgtgagg tcaatatgca ggacatcatt
    17281 cttctcaagc agggcgagat cgtcctcaag ggtctgaaca agaaatactt tgagcagaag
    17341 ctcatctcca acgtgaagcg ccgtctgcgc tcgctcggcg agtttgacgt aacgtgcacg
    17401 cagtccacga tctatgtgga gccgaagtcg gacgacatcg atatggacga aacgcagcag
    17461 gccatgacga aggtgttcgg catcgccgcc gtggtgcgtg ccgccgcgtg cgacaaaacg
    17521 cccgaagcga tggcgcagaa agccatcgag tacatggccg aggccatgcg cgaagccggg
    17581 agcttcaagg ttgaaacgcg ccgcgcggac aaggcctttc cgatgacctc catcgaagtc
    17641 agccagtacg tcggcggcga gcttgccgag gcgttcccgg agaccgccgt ggacgttcac
    17701 aaccccgagc tcaccgtgca cgtggaggtg cgcgagcgcg ccgcctacat ccaccgtccc
    17761 ccgctgcccg gcgcgggcgg tctgcccgtc ggctgcggcg gcacggcggt aacgctgctc
    17821 tccggcggca tcgattcgcc ggtatcgacg tatatgatcg ccaagcgcgg cgtacatatg
    17881 atcccggtgc atttcttctc gttcccgtac acctcggagc tggccaagca gaaggtgctc
    17941 gatcttgcca aggagctcac cgcctactgc gggcggctca ccgtggaggt cgtgccgttc
    18001 acgcacatcc agaccgagat ccgcgacaag tgccccgagg aatatttcac gctcatcatg
    18061 cgccggttta tgatgcgcat cgcgcaggag gtcgccgacc gaaacggtgc caaggcgctc
    18121 gtcacgggcg aaaatctcgg tcaggtcgcc tcgcagacga tggaagcgct cggctgcacc
    18181 gaggccgtca cgcgtctgcc cgtgctgcgc ccgctcatcg gctttgacaa ggaggagatc
    18241 gtccgcctgt cccgcaagat cggcacgttt gagctctcca cgctgcccta cgaggactgc
    18301 tgcacggtgt tcacgccgcg ccacccccgc acgcggcccc gcatcgacgc cgtggaggaa
    18361 gcagaggctg tgctcgatat cgagggtctc gtcgccgagg cgatggccgg catcgagcgc
    18421 gtgcgctttg atccctgatt ttctgatgta aggaggctcc tatgaacgcc gaaattctct
    18481 ccgtcgggac ggagcttctg ctcggcggca cggtcaacac cgacgcgcgg gacatttctc
    18541 tcgcgctggc ggagctgggc gtgaacgtat actggcacac cgtcgtcggc gacaatcccg
    18601 cccggctcac cgagtgcctt taccgcgcgc ggaaccgcgc cgatctcatc atcacgaccg
    18661 gcggtctcgg ccccacgtgc gacgatctca ccaagcagac gctcgcgcgc gcctttgacc
    18721 ggaagctcta tctcaacgag gacgcgctcg cggatatccg cgaatacttt gccgagcgcg
    18781 gccggagctt taccgacaac aacctccagc aggcatatct ccccgagggg agcatcatgc
    18841 tgccgaacga ctgcggcacc gcgccgggat gcatcatcga ggacggcccc gtgcgcgtcg
    18901 tgatgctgcc cggcccgccg agagagtgcg tcacgatgct gcggaactat gtgatgccgt
    18961 atctccagaa gctctccggc gccgtgatac gctcgcacat gatccgcttt ttcggcatcg
    19021 gcgagagcgc gatggaaacg cgcctgcgct cctttatgga cggggcgaac cccaccgtcg
    19081 cgccgtactc ccgcgagggc gaatgctggg tgcgcgtcac cgccaagggc gcgaacgagc
    19141 aggaatgcga agcgctcatg gagccggtga tcgaggatat ctgcgataag atgggcgatc
    19201 tcgtttacgg catcgactgc gcctcgctcg aagagcgcgt gctgcagctc ctgcaggaga
    19261 aaaacacgtc gctcgccgtg gcggagagct gcacgggcgg tctgctcgcg cagcgtatca
    19321 ccgatattcc cggcgcgagc gcgcattttc tcggcggcgt cgtgacgtat accgaggacg
    19381 ccaaggttcg tctgctcgat atggacgagg atctcatcgc cgaaaacggc gtcgtgagca
    19441 tgccggtcgc ggcaaagatg gccaagcgcg tgcgcaaggc gctcggcagc gacatcggca
    19501 tcggcatcac cggctgggcc gggcccgacg gggacgacgt ggggctcgtg ttcgtggcgc
    19561 tcgcctcgcg cggcggctgc tttgtccgcc gtctgcaatg cggccacgcg ccgcggccgc
    19621 gcatccggct catcgcggcc agcaacgctt tggatatgat ccggcggtat ctcaccggat
    19681 tggatgtata aaataaaaag gagacaacaa caatgatgac ctacaccatg gatatcgccg
    19741 gcctcaagcg cgatctgccg ctctgcccgg tgagcgacga tctgtatatc ggcgcgttcg
    19801 tcatgttcgg cgatgtggag atgaccatcc acgccgccaa ggagcttttg aagcgcgccc
    19861 cggagtttga ctacatcatc gcgcccgaag ccaaggcgat cccgctcgcg tatgaaatgt
    19921 cccgccagtg cggtattccc tatctgctcg cccgcaagaa ggccaaagcg tacatgaccg
    19981 gcattttcga ggtgcatgtg cactccatca ccaccggcgg cacgcagacc ctcattatcg
    20041 acaccgcgga cgccgagcgc atgaagggca agcgcatcct catcgtggac gacgtgatct
    20101 ccaccggcga gagcctgcgc gccatggaag agcttgtaaa gaaggccggc ggcaacatcg
    20161 tcggcaagat ggcggtcctc gccgagggcg gcgcgatcga gcggcacgac atcaccgtgc
    20221 tcgcccccct gcctctcttc aacccggacg gaacgctcaa gaactaatga aaaatgggag
    20281 ccgaatctcg gctcccatta aaatcccatc acatatgaaa aagcctccct tgtgtaaagg
    20341 gaggtggnnn nnnnnnncgg aggtggcgaa cccgaagggt ttgacggagg gattgtcgtt
    20401 ttccgaagac tacaatcccc cagtcagctt cgctgccagc cccctttgca caagggggcc
    20461 gcaaaaaggt cgttatcttt cccgcatccg caaaaagaaa ttccgcagca gcgcggcgca
    20521 ctcgtccttc ctgacgccgc cgtacacccg cggctgaaac ccgtagtttt caaaaaacag
    20581 gtcgatcacg ctgccgcacg aaccgctttt cgcttcccgc gcgccgtata ccaccgtggg
    20641 aatccgcgcg ttgagtatcg ccccggcgca catggggcag ggctcgagcg tcacatacag
    20701 cgtgcatccg tcgagccgcc acgtgccgag cgcacggcag gcctcgcgga tggcctcgat
    20761 ctcggcgtgg gcggtcgcgt ccgcgccctc ctcgcgccgg tttctccccc gtccgatcac
    20821 gttccccgcg ccgtcggtga taacgcagcc gaccggtatc tcccccgcgg cggcggcctc
    20881 acgcgcgagt gcgagcgcgg cggccatatt cttttcctgt gcggaggctc ccatgctttc
    20941 tgtcatactc atcggcattt ccctttccat ggatgcgttt gcggtgtccg ttacaaacgg
    21001 tatcgccatg cgcccgttca agacgggcta tgcgctctgg cacgggctgt atttcggcgc
    21061 gttccagttt ctcatgccgc tcgcgggtta cattctcggc agcacggtgt ccggctatgt
    21121 cacggccatc ggcccgtacg tgagctttgc gctgctcgcg ttcatcggcg gcaaaatgat
    21181 cctcgaagcc gtgcgcggcg gcgaggaggg ctccggcggc atggcggcgc tctcccacaa
    21241 gcggctgctc gcgctcgccg tggcaacgag catcgacgcg ctcgccgtcg gcgtgagctt
    21301 cgcgttctcg cccccgggcc ccggcgttgt actctcgtgc gccgttatcg gctgcacaac
    21361 gttcctcatc gccttcggcg gcgccatggc cgcgagccgc atccccggca tccgcccgaa
    21421 aacggcggag atcgccggcg gcatcatcct ggtcggcatc ggagtaaagn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctttttttc ggcgagtgcg ttaaaattgg
    21541 ttcaaccatt ttgtacagtt tcacgttttt ccgtttcatc ctctattttg tcttgattct
    21601 ttttcgtatt ggtgtatact ccgaatcaag aggtagggcc aatttgtgag tccatcggcc
    21661 ccggtatttt ctttttggct cggttgatat ataatgggca gcgatcaacc gcaggaggga
    21721 tctgccatgt attattcggg cgcgccgttt gctttcaaag aaaataaaaa cgtccgcagc
    21781 cttctggcct atatatccaa aaagggcttc tccgtgggcg acaagctccc gccggagcgc
    21841 gagctggccg agcagctcgg catcagccgc aactccctgc gcgaggcgct gaaggtgctc
    21901 gaggcgatgg acgttgtgga gatccgccac ggcagcggca tttttctccg gaaggaagat
    21961 acgcagcccg acgacggcag ctccctctgg ctgatgatcc acaaggacga gatactgaac
    22021 atcctcaccg tgcgcgaagc gctggatctc aaggcgatcg atcttatccc cgaggaggat
    22081 tacccccgcc tccgggacga gatgaaagcg tgcatcgccg ccgtgcgcaa aacggacatg
    22141 acgaacgagg acatgctccg ccacgacctg gaattccaca acatcatccg ccgcgccgcc
    22201 aacaacgaca ttcttttgaa catctgcatc acgctgaccg gcacgatcta cgacgagcgc
    22261 cgggtgcttt tcacccacac gaactatgtg gaacagtcgc tctgggagca caaccagatc
    22321 gccaacgcct tcggctcgct cgacgtcaat caggtcaagc aggcgtacat cgcccacctt
    22381 gccacgaccc gttcgagcat cgaactgacc cagccggaaa cctgaccgcc ctgtgcggcc
    22441 ggtttttata cagtaagtca tcctaccaat tttttaaaaa cagaaaacgg aggaaccacc
    22501 atgaaagaga tcaccgatct tcagcgcgaa atgctgaaaa agtaccatct ggacgccatg
    22561 gtcaccatga cgccggagaa catcacctac gtcaccggcg cttacatccc cacgcaggtc
    22621 acggtccgca gccgcaaggt catccacatc atcaccgaga agcacgaccc cgtcgtcatc
    22681 gttgtgaaca ttgaagagcc catcatgcgc gcccagagct ggatggatcc gaagagcatc
    22741 atcagctaca acgagttcac ccagaacccg atgatgatcg ccatcgacaa gctcaaggag
    22801 ttcggcatgg agacgaagcg catcggcatg gagctctcct atctctccgc catcgatatg
    22861 gatctcatgc acagggagct gccgcacgcc gagatcgtca acgccgacgc cgcgtacgaa
    22921 gagatgcgct tcatcaagat gcagttcgag cttgaccgca tcatctcctt cggctccaac
    22981 atcgaagagg tcatttacgg cgccttcgcc agcgcccgcg cgggcatgac tgagaaggac
    23041 atttaccgct tcatctccaa cggctttgcc gagatcgcca agggcgacaa gatgtccatg
    23101 ccgatggtcg gctccggcgt gcgcagctgc ctgctcaacg gcgagcccac cgacaaggtg
    23161 ctcgaaaagg gcgacatcgt ccgcgtggac gtgatgggca cgaaggacaa ctattactgc
    23221 gactgctgcc gcacggcggt cgtcggcacg ccgaccgagc accaggcgga gacctggagc
    23281 aagctcgtca aggcgcatga cgacgccatc gcccagatca agccgggcgt gagcaccaag
    23341 acgatctacg ataactttgc cagacagttc accgagtggg gcttcaagcc cgtcgcgttc
    23401 atcggccacg gtctcggtct cacgctgcac gaggagccgt acatcaacac ctacaaggac
    23461 acgacgctgc gcgagaacat ggtcctctgc gtcgagccga tccatgtgat cgacggcgag
    23521 tgcggctacc agctcgaaaa cgaggtcctt gtcacggcgg acggccaccg gatgatcacc
    23581 ggcacgcgcc atccgtacca gcagctcgcc accatccgcg cggacggata acaaccaaag
    23641 aggaggacgc atccatgaaa atcgccgttc tgggcggcgg acacggcggc tatgccgctg
    23701 cggccgacct tacgctgcgg gggtacgaag tgtgcctgtt caccttctcc gccgcgcgcg
    23761 tggaaacgct gcacaataac gataacgtga tctcctacac cggcgtctgg ggcgagggca
    23821 gctgccgcgt cgcccatgtg tcaaactcga tggaggaggc cgtttccggc gccgatctcg
    23881 tcgtgatgaa cgttcccggc accggccacg agcgctatct caacgccatg aagccgtatc
    23941 tgcgcggcga cacggtgctc tatatgaatc ccggtcatac cggcggcgcg ctgcgcgcgg
    24001 cgcacatcct gggccggggc cgcattgccg aggcgaacac gctcagctac atcgcccgca
    24061 aaacgagcga gacgagcgta cacgtaagca gcagcgacaa gcccgtcacc gtcggcgtgt
    24121 tcccggcgaa ggacaccgag gccgtgctgg aggtcatccg caaggcgtat ccgtatgtcg
    24181 tcgcgggccg cgacgtgctg gagtcctcgc tctgcaacat caacgccatg ttccatccgc
    24241 cgggcgtcgt gctcaatgcc ggctggatcg agcataccgg cggcgacttc cgcttctact
    24301 atgacggcgt gactccggct atcggcaagg tcatccgcat ggtggacgag gagcgtctgg
    24361 ccgttgcgaa ggcgtacggg cttgatctcg acaacttctg caccgtgctc tacaacgccg
    24421 gatccaccac gaaggaggcg gcggaggcca acgatcccta caaggcctgc caggagagcg
    24481 aagccaacaa gttcatcaaa gcgcccccct cgctcgatca ccggtacatg cacgaggaca
    24541 tcggctccgg ccttctcccg atcgcggagc tgggccgtct ggccggcgtc cccacgccga
    24601 tcacggatgc gcacgtcacc ctgtccgagg ccatgatggg ccgggattac cacgccgagg
    24661 gcgtgaatct cgagaagatg gggctggaag gcaaaacgat cgagcaggtc cttgattacg
    24721 tcacgcgcgg ctgaaccgcg gaaaggaaac acgctatgaa aatcgaatcg tataaaattt
    24781 ataaggtgag catccccacg cgccgccgcc acacctgggc gagcagcacc gccgacgtcg
    24841 ggcagggctg ggtcattctg gaagtcaaga ccgacgacgg catcgtcggc tacggcgaag
    24901 ccaccaccat gccggagtgg ggcggcgatt tcggccgcta ctacggcgaa tcgtacgaga
    24961 cgaccgaggt cgtcatccgc gacaacctct tccccgccat cgtgggcatg gatccctttg
    25021 acatcgatct catccatcac aagatggata aggccatccg cggctttgtc tacgccaagg
    25081 ccgccattga tatcgcgctc tacgacatca tgggcaaggc gctcgacaag cccgtttaca
    25141 agctgctcgg cggccggcac cgcaaggcca ttcccatcgc gcacagtctc ggcgtcgtgc
    25201 tcgacattga agccgccgtg gacgaggccg tcgccgcctg cggcgagggc atcaagacca
    25261 tcaagatgaa gggcggtctc gatcccgtgc gcgacatgga gctgatggaa aagctccgca
    25321 aggccgtcgg gccgcatatc aacatcctcg tggacgccaa ccagggctac cccaccgcca
    25381 aggacgcgat caagtggggc aagcgcatgg accgcttcga tctgcagtat ctggagcagc
    25441 cggtcgaggg ctttgatcag ctgcgcgaag tgacgcgcat gctcgaggtg cccgtgtgcg
    25501 ccgatgagag ctgctggacg ccgactgacg cgctcgatat cgtggcaaag cacgcggcgg
    25561 actacatctc catctacgtc accaaggccg gcggcctgta cccggcgcgc accgttgcga
    25621 agatcgccga gtgcgccggc atccgctgca acgtgaacgg ctcgggcgag ttcggcgtcg
    25681 gcacggcggc gaacctgcac atggcctgct gcggcgcgaa catcgacctt gcctccgtct
    25741 tcccgatcac ccatgtggag ggctgcccgg agcagacgaa ggtcgccgcc cgctggtaca
    25801 tggacgatat catcaccagg ccgttccagt acgacggcgg ctgcctgctc gtccccgatg
    25861 ggccgggtct cggcgtggag atcgaccgcg aaaagctcaa aaaatacagc gaaaccaaat
    25921 aaatacccgc atacgaaaag cgccggactt tccgaaaaaa agtccggcgc ttctccatgt
    25981 taaaaatccc cgtccgtttc cggacgggga tatgaggtaa aactatgtac tcaggcgagc
    26041 gcggcggtga tgatggccat cttgtagacc tcgtcggcgt tgcagccgcg ggagaggt
//
LOCUS       DAWAAE010000013        25837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_821_length_25837_cov_3.087193, whole genome shotgun sequence.
ACCESSION   DAWAAE010000013 DAWAAE010000000
VERSION     DAWAAE010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 25837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25837
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_25837_cov_3.087193"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5064..5073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9418..9427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13764..13773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15612..15626
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18977..18986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5180 a         8085 c         7635 g         4882 t
ORIGIN      
        1 ctcgcgccgc ggcacacgtt ccccgccgcg cacgaggacg ctttgacagg ctaccgctgg
       61 ctttgggaaa acgccgccgc ccttcgcatc gatccggcgc ggatcgccgc cgcgggagac
      121 agcgcgggcg ggaatcttgc cgcggcgctc tgccttatgg cgagggagca gcgcctcccg
      181 atgccgtgcg cgcagatgct gctctatccg gtgctggacg cgcgcatgga gacggagagt
      241 taccggcagt tcacggacac gcccatgtgc agcagccgcg atatggagaa gtactttgcc
      301 atgtacgcgc ccgatacgtc cgccgcgccg cgcgaatggc tctccccggc ggagagcgcg
      361 tcgctgcagg gtctgccggg cgcgtatatt gagacggcgg agtttgactg cctgcgcgac
      421 gagggcgcgc agtacgccgc gcatctcgcg cgcgagggcg tgccgtgcga gtatcacccg
      481 ataaaaaacg ccatgcacgg ctatgatatc gcggtaaaca gcgaatttct aaggcccgtg
      541 atggcgtatc gtatcgaatt tctgcgcaag gtttttacgg gaagataaag aggtgcagca
      601 gcaccgcgtt cacaatgccg agcaaaatcc cggccagcac ctgaatgggc gtatggccga
      661 caaattcctt gagcttaacg tccgtcagct tgccggagga gagctcgtca aacgcgcgga
      721 cgatctcgtt gatgatggca gcctgcttgc cggtctcgag ccggacgccc atcgcgtcct
      781 tgcagacgat cacggcgagc accgcggcga tggcaaactc gaaggagtcg agcccgtaga
      841 cgaatgccgc cgccaccgcg aacgaggaaa cggtcgcgga gtggccgctc ggcatgccgc
      901 cgtcgccgac gagccgggag agatcgagcc ggtgcgtgac gagcgcgtgg atgatcgttt
      961 tgagcacctg cgcccacagc cacgacgaaa tgccggtcag caaaaactgg ttggaaataa
     1021 gatcacgaaa ccacatggtt tccttcgcct cctgtgcttt ctgtatcgga agaaggcgca
     1081 cgcagatcgt cgagcgattc ttcgcacgtc tgcacataat cgctgtgcgc gaaccagaac
     1141 gcggcgatcc gctccgccgc ggcgtccgct tctccggcgt aggaacgcag cgtgaccgcg
     1201 ccgctcatgc gtctgcgccg tccggctcgg caacgtagct cacatactcg agcggcgtga
     1261 agccgagacg cttgtagagc gcgaccgcgc cggtgttttc cggctcgacc tcgagccgca
     1321 gacgcgccga gggaaactcc ttgcggacgg acgagaaaaa cttcgtaccg agaccgtgac
     1381 cgcggtatgc cttgcggatg tacagctcct cgatccagca gatcgggctg ccggactccg
     1441 gggaaaagct gcgcgagaga atggcgtagc ccgcggcttc gccgtccact tcaagaatgc
     1501 ggagaatgat gtacacgtcc gagcgcatgg cctcgcggaa cgtgttttcc atgtactgcg
     1561 ccgggatcgg gtgcatcacg gcgggggaat ggtaaaattc ctgcgccagt tccatataga
     1621 gggcgaagtc tttttcttca gcgttgcgga acatatcaaa aatatccttt ccggaatgcg
     1681 atgattccta tcggtaaagt ataccgcatt tgtccgggaa aaagcaaggg aacatatccg
     1741 ccgcctctgt ccggggcggt ttttttgttg aacgtttcat gccatgatct tctgcatctc
     1801 ccggcggaga cggagaagga tgcgcttttc gagccgggat atgtagcttt gcgaaatacc
     1861 catgcggtcg gcgacctcct tctgcgtata ctcccgccgc ccgagcagcc cgaagcgcag
     1921 ggagatgatg tcccggtcgc gctcgctgag ccttgccacg gcctcgtgca gcgcacggcg
     1981 gtcagcgtcg tcctccatcg ggcgggagac ctcatcctcg tcggtgccga gcacgtccga
     2041 gagcagcagc tcgttgccgt cccagtcggt gttgagcggc tcatcaaagc tcacctccgt
     2101 gcgctgcgcg gcgagcttgc gcagatacat caggatctcg ttttcaatgc accgcgaggc
     2161 gtacgttgcg agcttgatgt ttttttcgcg gtcaaacgtg ctcacggcct tgatgagtcc
     2221 gatcgtgccg atggagatga gatcctcgag ccccacgccg gtattttcaa accgccgcgc
     2281 aatatagaca acgagccgga gattgtgctc aatgagcgta cgttcggcgc ttttgtcccc
     2341 ggcgcagagc gccgtgagcg ccgcagtctc ctcctcgcgg gagagaggcg gcggcaaaac
     2401 ctcgcttccg ccgatatagt ataccggctc gttcggcagc agggcgagca gaagctgttg
     2461 cagcagatcg gaaaacatgg gcatgacctc cttatgatat tcaaacgagc gcggaatact
     2521 ccccgccgcc gagcggcgtc ggcgagaggg cgacgagata ttgctctgca atgccctcga
     2581 ccgtgacggc gtcgggacga aagcacacga gcagcccccg cttcgtgccg acagcggaaa
     2641 agccgacgag ccgcagccgc cgcgagagca ccgggtgtga ggacaggacg cgaaagcatg
     2701 ccgccggatc gtccggcagc ggcagaacga gcgggggagt aaagagcgaa gcgagagcgc
     2761 ccggctccgc gagcaggatg cgcgagccgg agagcgggtc gaaaagcgag ttgccggtgt
     2821 cccgcagcgc ccgcagggaa acggttttgc cgcaaagctc gatcttcgct tccacgacgc
     2881 agcgctcgcg gcgctttaga aaccgcccga aaaagagccg caccgcggca tagcacagcc
     2941 cgaaggagag cgcgagcgtt ttgagcgaag cgccggagag cacgccgcgc acctccgcgc
     3001 cggagagcag cgatatgccg tacaccgccc cggcgaacgc cgaggagagc gcgagaaagc
     3061 agccccagcc gcgccagagc tccggcggcg cgccgaacgc gatccacgcc atgagcagcg
     3121 aaacgagcgc cttcaccgcg agcgagacga gaaacgagag cggggggatc gcggcgaaca
     3181 cggcgtacac cccgccgagc gccgcggccg cggcaaagcg tgtgcgcctc agcgccctcc
     3241 cgcgcagccg cgcgcacgag agcaaaagca gatagtccgt gagcgcattg agaaaaaaga
     3301 gctcatccac atagatcacg ttcatgctcc aagcataccc tgccgggggc gaaaatgttg
     3361 tccgaacggg gacggggtat cttgcaaagc ggtgacggga tgtgtataat atttcggtaa
     3421 atcgtaatga acccgtgaaa ggaagaaata gagcatgtca cagtaccgta cccacacctg
     3481 cggggagctc cgcgccgccg acgcgggcaa aaccgtaacg ctggccggct ggatggagaa
     3541 tgtccgtgag gtcggcagca acttcgcgtt cgtcgtcctg cgcgattttt acggcacgac
     3601 gcagatcgtc gtcgattccg ccgagatgat gaagaccgtc aagggcatca acaaggagag
     3661 caccatcgcc gtcaccggca cggtccgcct gcgcgagagc cccaacacca agctcgccac
     3721 cggcgagatc gaggtcgtgc ccgagaagat cgagatcctc ggccgctgcc gctacaacga
     3781 gctgccgttt gagatcaacc gcagccgtga ggccgacgag agcgcgagac tcaagtaccg
     3841 ctatctcgat ctgcgcaacc ccgccgtcaa gaacaacatc attctgcgct gcaacgtcgt
     3901 cgcggcgctg cgccgcgcga tgatggacca cggcttcctc gagatcacga cgccgatcct
     3961 caccgcctcg tcccccgagg gcgcgcgcga ctatcttgtc cccgcccgca agcaccccgg
     4021 caagttctac gctctgccgc aggcgccgca gcagttcaag cagctgctga tgacgagcgg
     4081 ctttgataag tatttccaga tcgccccctg cttccgcgac gaggacgccc gcggcgaccg
     4141 ctcgcccggc gagttctacc agctcgatat ggagatggcg ttcgcctcgc aggaggacgt
     4201 gttcgcggtg ctcgaggacg tgctgccgcc gatcttcgca aagtacggca cctacgatat
     4261 cgcctcccag ccgccgttcc gccgcatccc gtatatggag gcgatggaca agtacggctc
     4321 cgataagccc gatctgcgca tcgatctcac cgtgacggac atttccgata tggtgcagga
     4381 taccggcttt gagccgttcg ttggtcagat cgtcaaggct gtgccggtct ccggctgcac
     4441 gctcacccgc aagcagatcg acaagctctg cgcggacgtg gaagtccagg cgggccgcaa
     4501 gccctactgg ctgcgcatgg acgagcacgg cgcccttgtc ggcggcgtgg cgaagttctt
     4561 tgcgggcaaa gaggacgcgc tcagggaagc cctcgctctc acgcccgata cgctcgtgct
     4621 catctccgcc ggcaagaaga ccgaggcgca gaaaaccgcc ggcgttatgc tcaaaatggc
     4681 gggcacgctc gtccccggcc acatggacga gaagcgctac gagttctgct ggatcgtcga
     4741 tttcccgatg tttgagatcg gcgaggagtc cggcgagctt gagttctgcc acaacccgtt
     4801 ctcgatgccg aacggcggtc tcgaggtgct gctggccgcc gagcgcggcg agctcgaccc
     4861 gcttaccatc atggcgtatc agtacgacct cgtgtgcaac ggcgtggagc tctcctccgg
     4921 cgcggtgcgt aaccacgatc ccgagatcat ggtcaaggcg ttcgagatgg tccgtctcgg
     4981 cgaggacgac gtaaaggcca agttccccgc catgtataac gccttctgct acggcgcgcc
     5041 cccgcacgcc ggtatcgccc cggnnnnnnn nnnagatcat tccgttcccg atgaacaaga
     5101 acgcgcagga cgtgatgatg ggcgctccct ccaccgtgga gcagaagcag ctcgatgagc
     5161 tgcacatcgc cctcgtcggc cagctcgaag aataagaagc aaaaagctgc ggcgcatcct
     5221 tcgggatgcg ccgcttcctg tttgccgcgc tttttcgttg acggcggttt cttttgcgtg
     5281 tagaatcgtg tcggaaggcg gtattctcta taatgctgcg cctgctgcgg cgaaaagatc
     5341 cccgcggaaa gccggttctg ccccaggtgc ggcgcgccgc tcccgccgcg cagtgaaaaa
     5401 caataaactt actaaaggaa gaagaccgta tgaaaattcc gtgcaaaaag ctccgtgaaa
     5461 atgccatact gcccacgcgc ggcaccgcgc tcgccgcggg cgttgatctt tacgccctgc
     5521 tgccggacgg gcaggagagc gcggccgttg ccccgcaggg caagtacctc ttccccaccg
     5581 gcatcgccgt ggagatcccg gagggatacg tcggactggt gtttgcccgc agcggcatcg
     5641 ccacaaagcg ccagctcgcc cccgccaact gcgtcggcgt gatcgacgcc gattaccgcg
     5701 gagagctgcg ggtgtttctg cagaacaacg gcaccgagac gcagaccgtg gaaaacggcg
     5761 agcgcatcgc gcagctcgtc atcacgccgt gtcttgacat tgagatcgag gaaacggacg
     5821 agctctccga aacgctccgc ggcgcggcgg gtttcggcag caccggcacg cactgaagcg
     5881 gagccgaaaa accgtcacag aaaaaacatc gccccgcatt ttggacgcac tatccaaaac
     5941 gcggggcgat tttttgtatt ctttcaccga aataaagaca gacttgcctg cacgatgcgc
     6001 cgcacgaacc gcacgaccgt ttcgcgctca taccggtcgt agccctccac cacgacatgc
     6061 atcagcccat tggacagatg cgtgtacagc atctccagtt cctcgtcgct cgcgcgcagc
     6121 tcctgccgcc agtaggcgat gctctgcggc cgggcgtaat cgatcatccg gtggatgacc
     6181 gtcgagccgg gattttggaa gatcagcaca cggcaggctt cctcgttctt cccgatcatg
     6241 tcgtagatcg cctcgatcag cgccgcaaca tcaaacgatt ttatcagcgt gagcgactgc
     6301 gcgaacgctt caagcagctc attctcgatc gcctccagca gcgcaaagca gtcgctgtaa
     6361 tgcgaataaa acgtcgcgcg gttcagctcc gccagctcgc agacctcctt gaccgtgatc
     6421 ttgttcacgc tcttttcctt cagcagcgtt aaaagcgcct gcttgagcgc gtgctgcgta
     6481 taccggactc tcgcatcgga ttttcctgcc atgggacacc tcatcacaaa ttgtttacag
     6541 aattgggcaa cgtttctgtt ccgcatgtcg gttttctttc acgcggcagc cggttgacgg
     6601 ttgtacatct ctgtcggaaa aagtatacta tatacatgaa agataatcaa catatgtctt
     6661 tcgtatttat cccatggagg tggatcccta tgtataaaac ttatcttgtg accggcgcga
     6721 ctggctttct tggccgcacg gtcgtttcgg agctgctctc ccgcggtgcg gcggtgcggg
     6781 cgctcgtact gccgggcgac cggctcgccg gagttttgcc cgagagcgtg gaacgcacgg
     6841 agggcgatat ctgcagcgcg tccgcgctgg aaaatttctt tgccggaacg gatgagagca
     6901 cctgcgtcat ccatacggcg ggcatcgtaa cggtggcatc gcgcccggac gcctcgctct
     6961 accgcgtaaa tgtggacggg acgcgcgcca ttttgcgcga gtgcgccgcg catggcgtcg
     7021 gcaagctcgt gtatatcagc tccgtccatg cgctgcccga aaagcccaag ggcatgacga
     7081 tcacggaaac cgacgccgtt tcttcggaat ttgtttccgg cgattacgcc aaaagcaaag
     7141 cggaggcgac atcgctcgta tttgccgcag cgaaaaatgg tctgaatgcg agcatcgtgt
     7201 tcccgtccgg cattatcggg ccgggcgata tcgccggagg cagcatgacg acgatgttcc
     7261 gctcgttcct tgcggggcgg ctcccgctcg cggtgcgcgg cggatacgac ttcgttgatg
     7321 tgcgcgacgt tgcccgcggc atcgccgact gtgcagagaa cgggcagcgc ggctgcggtt
     7381 acattctctc cggccactat acaacgatcc gcgatatgct cgaagcgctc aaatccgcag
     7441 ccggtgtacg gcgcacggta acatatctgc cgctcaggct tgcccggagc gcggcgaagc
     7501 tctgtgagaa atttacccgt aggggaaaga agcctttctt cacgccctat tccatagcag
     7561 tcctcggctc gaacgcggat ttcagccaca gcgcagccag ccggacgttt ggctatacac
     7621 cgcgtccgct gcttgcgacg ctgcgcgaca cggcgcactg gctgtgccgg gcaaagccta
     7681 tcacggcagc aacatgaaac tataagcccc atactccgcc ggcggaagcg catcaagcgc
     7741 ttccgccggt ttcgttttcc acgcgccgct ccacatcccg gacgagcgct tcaaaccgcc
     7801 gggcagtatc cgggctgtcg gactcaatgc agagcgccgc gcggtcggcg cagggcgcga
     7861 tgtgcacgcc gccggggacc gtccgtacgg tcagcccgtc ggtgagatcc gcgcccgctt
     7921 ccatgagtcc cgcggtgagc gcgcgcatga gccgcgcgcg gtgcggcgcg ccgcagtaca
     7981 cccggcaggc gtaccgcgcc cgttcggaaa aagcctccgg cgtgcgctca agtgcgccgc
     8041 ctctctgctc gggcaggcgc agtttgccct cccaggcgtc gagattggcc cggtagtacg
     8101 agccggggtc gccgatatcg cgccagtagc cctccatcgg cacgccgcgg atgccgcgcc
     8161 cggacgccat cagccgcggg aaaagatcgc gggcaaaatc aaagggcgta tccgccggga
     8221 taaagcgcag cacctccggg gacagaacat agatgccggt gtttaccaga tcggtaacga
     8281 cgcgctccca cgcaggcttt tcgatgaagc tgcgcaccca cccctcgccg tcggtcacgg
     8341 cgagaccgta gggcagcggc gcttcgtgcg gatagagcgc catcgtaacg gcggcgtgcg
     8401 cgcgccggtg ctcgttccag agggcgcgaa gatcaaaatc gcacgcggcg tcgccgctga
     8461 tgatgagcgc gttttccccc gcgataaaat cctcgcagtt tttcaccgcg cccgcggtgc
     8521 cgagcgctct gtcctcgcgc cggtactgca tgcgcacgcc gaagccgctg ccgtcgccga
     8581 agtaccgctc gatctgctcg ggcaggcagg ccgtcgtgca gcagagatcc cggaagccgc
     8641 agcgcttcaa aagcgctacg atgtgctcga gcatcggcgc gccgagcagc tccgccatgg
     8701 gcttcggcac cgctccgtga gtgaccgggg cgagccgcgt ccctcttcct cccgccaaaa
     8761 tgaccgcttt catcttttta taaaagctcc tttttttatt tttacggagg aagtttctgc
     8821 atcgcggcga aaaaatatgc aaacagcgga agaatgcggc gaaaagggct cgcattcttt
     8881 ggatatttgt ggtatattat attctgtatt tttatctgcc cgctctgtgg ttccggcggg
     8941 catggggaga ggatactttg aacagccgca aaccgcagaa atttgccgag cccggcctcc
     9001 gcatgtgcct gctcatcatg ctcatattcg cgcttgtaac cttctttttc gcccgcttca
     9061 accgctatat cgcctacggc gaggcggtcg tcacggtgct gctcgcgatc tacgcgctca
     9121 tccgcgcgag agcgcgccag aaggagctgc aggcgttcgt tgagaacgtc acctacaacg
     9181 ccgaatccgc cacgaacaac acgctcatcc acttcccgct gccgatggcg gtgttccgtc
     9241 tgaacgacag cggcgtcgtc tggggcaacc agccgttctg ggagatgtgc ggccgcacga
     9301 gccccgcgtt cgacgcgaag ctcgcctcgc tcgtgccgga attcaacagc aagtggctgc
     9361 tcgagggcaa aaaccgcatg gcggatcttc tcgaggtaga cgggaaaaag tatcaggnnn
     9421 nnnnnnntca acggcaacat ggtccgcgcc ggagagcgcg aggagaccta tgagttcatg
     9481 ggcatcacct actgggtgga cgtgaccgag tacgacgacg taaaacagga atatctcgcc
     9541 acgcgcccgg tcgtgatggt catgctcgtg gataactacg acgagctgat gaagccgctc
     9601 accgaccgcc agcgcaccga gctgcgcggc cagctcgaca ttgccattga gaaatggtgc
     9661 gagggacgcg gcggcattct ccggcgcgtg gaccgcgacc ggtacatctt catttttgaa
     9721 aagcggcatt ttgacgagat cacgaaaaac cgcttcacgc tcgtcgagag cattcattcg
     9781 atcgtcaatc tcacgggcat ccacgccacg gtgagcatcg gcgtcggact cgacggcgcg
     9841 agctatgacg aggactattc cttcgccacg ctcgccgagg atatggcgct ctcgcgcggc
     9901 ggcgatcagg cggtcgtgaa aaacaagttc aactttgaat ttttcggcgg gcgcggcgcg
     9961 gaggtcgaaa cgcgcacaaa ggtcaagtcc cgcgttatgg caaactctct ctcccggctc
    10021 gtgcaggacg cctcgcaggt gttcatcatg ggccaccggt acgccgatct cgacgccgtc
    10081 ggcgcggcgg tgggcgtgcg gtgcattgcc cggcgcttcc actgcccggc gaagatcgtg
    10141 ctcgacatgg acaaaaacgc cgtcgggccg ctgctcacca tgctgcacgg cgaggaggaa
    10201 tacaaaaccg cctttatcag cgcgcaggag gcgctgctcg aggccgacag ccggacgctg
    10261 ctcgtcgtgg tggacacgaa ccgccccgaa caggtggagg acgaatcgct gctcaccgcg
    10321 tgcagcaacc ggctcgcggt catcgaccac caccggcgcg ccgcgaccta tatcaaaaac
    10381 gccacgctca cgctctatga gccgaacgcc tcgtccacgt gcgagctcgt cacggagctc
    10441 atacaggagc tgtgcgagcc gacggatatt ctcccctttg aggcggacgc cgtgctctcc
    10501 ggcatcgtgc tcgacacgaa aaactttacg atccgcaccg gcgaccgcac gtttgacgcc
    10561 gcggcgttcc tccgccgcag cggcgcggac accacgcgcg tgaagaagct cttccagaac
    10621 ggcatggagg agaccatgga gcgctacgac attatgaagc aggcgcgcat ttacaagggc
    10681 atcgccgtgg tcgccgcgca ggagacgcag aaccgcatcg tcgccgcgca ggccgcggac
    10741 gagcttttga acatctccgg cgtgaacgcg agcgccgttt tgtaccccac cggcgacggc
    10801 ggcgtcgcgg tgtcggcgcg gagcatcggc aatgtgaacg tgcaggtgct gctcgaaacg
    10861 ctcggcggcg gcggcaaccg ctcggcggcc ggggcgcaga ttccgaactg ctcgctgcgc
    10921 gacgcggtga accggctctt cgccgccatc gacgaatact gcgcgacaga ctaataaaaa
    10981 aactaagaga ccgaaggagg tcatattcca tgaaagttat tttccagcag gatgtccgcg
    11041 gacagggcaa aaagggcgag atgaaggaag tctccgaggg ctacggccgc aactaccttc
    11101 tcccccgcgg tctcgccgta gaagcgaata aggacaatct caacaccctc gcgctcaagg
    11161 aaaaggcgcg gaaggcgcag gaagcgcgcg agcgcgccga ggccgaggaa gccgccgcca
    11221 agctcaagga cgttatcgtc acggtgcgcg ccaaggccgg cgcgaacggg cgtcttttcg
    11281 gcagcgtcac gtcgcaggag atcgtggacg cgctgcgcga gcagcacggc gtctccatcg
    11341 agaagaaccg catcgtgcag gccgagccga tcaagagctt cggcagcttt gaagtcaagt
    11401 gcaagttcgg ctacggcatc gacggcaccg tgcagctgct cgtcatcgaa aagtaagagc
    11461 ggatggaaga actgctcgga cggcgggtgc cctggtccgc ggaagcggaa caggcggtgc
    11521 tcggctcgtg cctgatcgac ccgtcctgcc tgccggacgt tatgacggcg gtgcgcggcg
    11581 cggatttcta tctgccgcag aaccgggaga tctttgaaac gatcttctcc atgttcacct
    11641 tcgccaagcc catcgacccg gtgacggtgc tcgatgagat gaaggtgcgc ggcgtttacc
    11701 atgacgagag ctcgcagcag tacatccgcg agctgatgcg cttgacgccc acggcggcga
    11761 acgtgctcaa atacgccgcc atcgtgcgcg accaggcgct gctccgcaac gtcatcaccg
    11821 tgtgcgagga aacggtcgag gccgcctcct ccggcgcggg cgaggccggg gatgtgctcg
    11881 atctctgcga aaagaagatc tatgcgctcc ggcaggaccg cgttgtcggc gggctgatgc
    11941 cggtgtcgca ggtcgtgaac agcgtctact ccaatctttc cgagctcgcc gcctccggcc
    12001 agccgatccc cggcatcccg accggcttcc gggacgtgga ccgccgcatc atgggcatga
    12061 acaagggcga ctttatcctt gtcgccgccc gccccggcat gggcaaaacg agtatcggtc
    12121 tcaacatctg cctgaacgcc gcgaaagcca cgggaaaggc cgtcgccatc ttctcgctcg
    12181 aaatgagccg cgagcagctc gtaacgcgct ttctctccag cgagggcgag atcccgctcg
    12241 ggaaccttct cacgggcaat ctcacgatgg acgagtggaa gcgcacggcg cacgcctgcg
    12301 ccgcggtgtc ctcgctcgat atcctcgtaa acgacaaccc gacgctcacc gttgcggaaa
    12361 tgaacgcgca gtgccgccgg ctcaacaatc tcggccttgt gatgatcgac tatcttcagc
    12421 tcatgcaggg ctccggcagc aagaacagct gggcaagcga gagccgccag caggtcgtca
    12481 gcgacatttc gcgcatgatg aagatcatgg caaaggagct gaacgtgccg gtgctgtgtc
    12541 tttcgcagct ctcgcgcgcc aacgagcagc ggcaggacaa gcgcccgatg ctctcggatc
    12601 tgcgcgagtc cggctcgatt gagcaggacg ccgacatcgt catgggcatt taccgcgaag
    12661 gctattacaa ccaggagatc gaaaacccga atctcacgga gctgatcgtg ctcaaaaacc
    12721 gccacggcga gaccggcaag gacagtctcc agtggcaggg cgagtataca acgttccgcg
    12781 acgccgaccg ctggcacaat gagtgagccg gtgctcgacc gcagcctgct cccggcgggg
    12841 tgcacggtgc tgtgcgccgt gtccggcggg gcggactcgg tatgtctgct cgatctcgtg
    12901 cggcggctcg gcgatgtgac ggtgctctgc gcccacttcg accacggcat ccgcggcgcg
    12961 gagagcgcgc gggacgctgc gttcgtcgaa gcgctctgca aagaatgggg cgtcccgttc
    13021 tttcccggcc ggggcgacgt tccggcgtat gccgcggcga acggcttgag catcgagacc
    13081 gcggcgcggg aggcgcgcta cgcctttctc gagcgcaccg cgaaggaaca gggcgcggac
    13141 gtcatcgcca cggcgcacaa tttaaacgac aacgcggaga ccattctctt ccgcatggcg
    13201 cgcggcacgg ggctgcacgg acttaccggc atcccggcgc ggcgcggcag gatcgtccgc
    13261 ccgcttctgc agacgccgcg gcgcgatatt gaggaatatc tcaccgcgcg cggcatcccc
    13321 catgtggagg actccacgaa cgcggagacg gacgctgcgc gaaaccgcat ccggctcgac
    13381 gtgctcccga gactcgagag catccacccc ggcgcggcgg cgggcatcgc gcgcatgagc
    13441 gaaacgctcg ccgaggacga ggcgtttctc gcctcgctcg cggaggaaaa gctcgccctc
    13501 tggggcgagg cgatccccgg cgcggagctt tgcgcggtgc cgcggcccgt cgcgcggcgc
    13561 gctcttgcgc gctggctcgg cggagaactc tcgcgcgagc ggttcgacgc gctgctgcgc
    13621 tttgccgccg gagacggcga cggcgtgctg gagcttccgg gacggcgcgt ccggcgggag
    13681 ctcgggcagc ttcggctcgg cgagggggac gcgccgccgc tggaggagcg tgtgcttttg
    13741 cccggagaaa cgctccttta cccnnnnnnn nnncctttac cccggtcggt ggcgcatttc
    13801 gtgcgaaagt tgcgctgcgg ggtcggaaat tcaaacttcg ttcaacactt tttgcttttc
    13861 atgtgccaat atatgtggta aactgtccgt cgcatcccgc gccgcgggag atacgatcct
    13921 cctgcaccgc cgcggcggta cgcgctcggt gagcaagctg ctcggcgaag cgcgcatccc
    13981 ggtctcgcag cgcgccgccg ttcccgtttt gcgcgacggg gcggggcctc ttgccgttta
    14041 cggcatcggg cagagcgaga gagctttccc cgcgccgcag gaaccgtttt ataaaatcac
    14101 tatttcgcca atttcggagg aagagagaga atgagagaag atattgcgtc catactgctc
    14161 agtgaggagc agatccatac gcgcgtccgc gagctgggcg cggagatctc ccgcgattac
    14221 gccgggaaag agccgctgtt cgtcggcgtt ttgaagggct gcttcatttt catggcggac
    14281 ctgttccgcg aagtgacgat ccacggcagc gtggacttca tggccgtttc gtcctacggc
    14341 aacggcacct ccaccacggg cgcggtgaag atcaacaagg atctcagcca ggacattgaa
    14401 aaccgtgatg tcatcatcgt cgaggacatt ctcgactccg gcatcacgct cagctatctc
    14461 acgaaatatc tccagaaccg caagccgagc tccatctgca tcgcaacgct gctcgataag
    14521 cccgcgcgcc gccgcgccga tgtgcaggcg aagtacgtcg gcttcaccgt gccggacgcc
    14581 tttgtcgtcg gctacggtct ggattacgcg gaaaagtacc gcaatctgcc gttcatcggc
    14641 gttctcaagc cggaagtata cgaaaaataa atctctgatt tatatattgt ccgcgccgtc
    14701 cggtgggacg gcagaaaagg aagtgacagt ttttgaacaa ccgaccctcc cccaacaaga
    14761 cccgtcagat cggctttctc gtattgatct tcgtgctgct gctcggcacg atctattcca
    14821 tgacgagaag caccgcgaag gaatcgcttg tctattcgga agtcatcgag ctttttgaga
    14881 cgaagcaggt cgaatccttc accatcgaca ccgacggcaa tctcaccatg cagctgcgct
    14941 ccgcctacaa ggggcagacg aaatactcct acgagtgcgg cagcttcagc atcttctaca
    15001 acgatctcaa cgatctcgtg caggagcaga aggccgaagg cattctcacc gagtacgact
    15061 acccgcccgc gtgggaggcg gcctggtggc tgagctttgt gccgtatctc atcatcttcg
    15121 tcatcatggg cgtgctgtgg tactccatga ttcgctcggc gcagggcggc ggcggtgccg
    15181 gcggcgttat gaagttctcc aaggcgcgca cccgtctcgg cagcgaggaa aagcagaaaa
    15241 agacgtttgc cgacgttgcg ggcgccgacg aggaaaagga agagctgcag gagatcgtcg
    15301 aatatctgaa aaaccccgcg gcgtattccc agatgggcgc gcgcatcccc aagggcgtgc
    15361 tgctcgtcgg ccctccgggc acgggcaaaa cgctgctcgc caaggcggtc gccggcgaag
    15421 cgggcgtcga atttctgtcc atctccggct ccgacttcgt tgagctgtac gtcggcgtcg
    15481 gcgcgggccg cgtccgtgac ctgtttgagc aggccaagaa ggtcgccccc gccatcgtgt
    15541 tcatcgacga gatcgacgcc gtcggccgtc agcgcggctc cggtctcggc ggcggccacg
    15601 acgagcgcga gnnnnnnnnn nnnnnncgct caaccagctg ctcgtggaaa tggacggctt
    15661 caacgataac cagggcgtca tcgtcatggc ggcaacgaac cgcgccgaca ttctcgataa
    15721 cgcgcttctc cgccccggcc gctttgaccg gcaggtgtac gtcggtctgc ccgatatcaa
    15781 gggccgtgcc gacattctga aggtccatgc ccgcggcaag ccgctcggcg aggatgtgga
    15841 tctgcgcgac atcgccaagg gcacgcccgg cttctccggc gcggatctgg aaaacctgct
    15901 caacgaggcc gcactgctcg cggtgcgccg tcaccgccgg ttcatcatgc agaaggacat
    15961 tgacgacgcc attctcaagg tctccatggg cccggagaaa aagtcccgcg tcatcaccga
    16021 gaaggagcgg cggctcaccg cgtaccacga gtccggtcac gccatcgccg cgcattatct
    16081 tgagcatgtc gatcccgtgc agtatatcac gatcatcccg cgcggtcagg ccggcggctt
    16141 cacgctgttc cgcccgcagg acgacaagag cttccgctcg cgcagcgaga tgtttgagga
    16201 catcgtcgtc gcgctcggtg ggcgcatcgc cgagaagatc ttccttgacg acatctccac
    16261 cggcgcttcc ggcgacatcc agcaggcgac gcacaccgcc cgcaacatgg tcaccgtgta
    16321 cggcatgagc gaccgtctcg gccccatcag cttcgattcc tcgggccaca gcatcttcat
    16381 cggccgcgac ttcggccaga cgaagagcta ttccgaagag accgccgcca tcatcgacga
    16441 agaggtcaag cacatcttcg acgaggccgc gctcaagtgt gaggagatcc tccgcgccca
    16501 cgccgacgtg ctcgttgcac tcgcggaata tctgctcatc aacgagacga tcgacggcga
    16561 ggacttccgc tacttctgcg atcacaaatg catgcccccg ctgccggaga aatccgacgc
    16621 cgtgaagcag aaggaagccg agcttctcaa ggaaaccgaa acgcctgccg cccccgccga
    16681 gaacgcggaa gcgaagagcg aaccggacga aaacaaaaaa gaagagtaaa gcaaaagccc
    16741 cgcctgttgg acaaacatcc aacaggcggg gcgttgttat ctatgaagag gacgaagccc
    16801 tcggtctcag caagtccccc ctattacggc agcgccttca gccatgcatt ccaacgttgc
    16861 tccagtactc ttccagtgcg gcgttgattt ttgcgggatc cattacgtcc gtgggcatgg
    16921 gctggctcaa atcgatctcc ccgtcgccgc acgggaggaa aaatacttga tctcttaagc
    16981 ggaatatccc atacagatca taggtcgttc cgcccagtct gtttgttccc tccaagtcca
    17041 cgattatgcc ctgtaccgta tcactgctgg cgggattatt atatatcttc cgaatgcgca
    17101 ccgcagaggc attataaaag ctctccagca tggtgccggt caatgtagtc gaacagctcc
    17161 cgctccgcgt cgttgagctg ttcatacgcc gtcggcgcgg caaccgtgac ggagcaggcc
    17221 gctgttttgc cgctctcgga caccgcgaga atgttcgtat tccccggcgc taccgccgtc
    17281 acgacgccgt tctcatccac cgttgcaacg ctctcgtccg cggatttcca gccgtagctc
    17341 tccgttgcgt tctccggcgc gacaacggcg gaaagcgtta cgttctctcc ctctttcagc
    17401 tccgctgcgt tttttgagag cgtgatgctc tcgattttca cctcaccgca ggagcaaaac
    17461 aggatcgcgg tcaaaagagc cgccgcagtc aggcgaatgg gctttttcat ttcagtttgc
    17521 ctccgtccac gcgtcataga gggcaagaac atcggcaatc gcttgcttgt cttttgcgga
    17581 gatcgtcata tcatagatat atttcccgct gaaacgcacc tctgtttctt ccgacgcagc
    17641 tatgcttcgg aaaagctgga tatctgaatc gctcacattt gcggtgttgt agacttccca
    17701 cacgtttccc caagcattat cgcgcacaac agcattatag ccaactgatt tcgtatactt
    17761 tgttccatct ataataaacg tcatggtgtc ccagaatatc caatcgtcac cagtataatc
    17821 acaataccag caaagatagg tatacccgga atccgttcgc ccaatatatg ggataatata
    17881 cgaacgtgta tcgtcatatt gcggtactgt ggtgtgcgta taaaatgtca cctgattgaa
    17941 gcggtcctct tcagcactca tctttttcag gagcttttca atcactgcgc tgcgttcttt
    18001 ctcttttaac tcacgcagtt gcttttccgc cgccgtcagc gcctctgcat cgacttttcc
    18061 cttggccaaa tcatcaagcg cgtcgtatgc gctgcgtgcg tcccggatag ccggttcgct
    18121 ctccagcgtc acctcttcac ctatggcgtt gatctgttcc gtgacttttt ctgctgcctg
    18181 cgccgccata agttcggcat aggcttcttc tgctgcggtg agcacatcgg cattggacac
    18241 ccgctcgcgc agcgacgcgt cacagctgtc aaacgccgag cgcgccgccg cgatcgcgtc
    18301 gccgctgctc aattcaactt ttccaatcga agaaatcgca ttttctactg ccgcgatctc
    18361 cgcaagccgc tgttcctcaa cgagccggtc atactcttct ctcgcggttt gcagcaggtt
    18421 tctgttgtcc agagaattcc ggtctttctc ttcaagcgac tccaccgccg cctccgccgc
    18481 ggcaatggcc gtctcgcttt ccaaggtgac ttcgccgatg gctgcgatct gctcatccac
    18541 cgctgtggca gcttcgcttt ttccgcaggc gcagagaagg aggcttgccg ccaacacgag
    18601 gatcaaaata tgccgttttt tcattttctt ccctcactgt ctttttgtcg ttttggcgca
    18661 gtacgcgtcg tatttgcagt atagcaaaac atttgtaaca ttacaagtta taaatatatc
    18721 tttttacgtt gtcgcaatgc gtgtcgcctg cgggtagact gaataagggt gagcgcatga
    18781 gaaacaatca gttttccaaa taccggagtc tcgggctgaa tatcgcttat taccgcaaag
    18841 accgcggtct ttcgcagagc gagctggccg agcgcgtgaa catcagccgc acgcacatga
    18901 gccggatcga aacggcggac tgcgccgttt ctctcgacgt gattttcgat atctgcgacg
    18961 cactggagat aaagccnnnn nnnnnnattt tcgggcatga agaaccgccg cgaacgcaag
    19021 cgtttgcggc ggttcttgac agcacgagcg gagcagagcc ccgcccctac aagaccgtat
    19081 taaaatccgt ccgtagggga ggggctctgc tcctccccat ttctctccgt cagcgagaaa
    19141 tcttttcaca tcggtaatgc aggaagccgt tctcctgccg gataaacaca aacccgacct
    19201 tttcgagcac gcgccggctg cgcgtgttct tttctatgac ccgcgcggtc acggcggtca
    19261 ggcccagctc gtcaaaggca tatgcgagca gaagccgctc cgccgctgtg ccgataccgc
    19321 ggtttttcac cgcgtcgttc tggagatgga tggaaagctc gccctcccgc ttttcccagt
    19381 cgatatgctt gatgccggtc tcgcccaccg gacacccgtc gagccgaatg agaaaatgcc
    19441 gccggtcggg cgcgccgtag cgctcgaacc gctttcgcgt ctcttccgga gagtagcgat
    19501 acgcggaaaa gaggctcatg tccgcaaacg tggccgggtc gcgctgaaaa tcgcggtcaa
    19561 gctcgttcat tccttcaagc gtgatctttt ccagagcaat gttcatgtcc tccgctcctt
    19621 tcctccggcg ccggcaccag cccccacgcg agccacagaa gcggcgcgct cacgacaagg
    19681 ccgagcgcga aaaatgcctg catccagtac gccgcggcgc cccagagcca cgcggcgctc
    19741 tccccgttct cccgcgggcg gaaaaacgtg acgcccacca tgccgaaata aagcgcgagc
    19801 cccggccacc cgagattcat cgcatagccg agatactcgt tgtgcgcgtt cgtaacatac
    19861 gttccgagcg tctgtcccgt ctccggcacg aagcgcgtga actcgatgtg cagccgcgcc
    19921 gccgccgtgc ccggcccgcc gccgaacacc ggccgctgcg cgatgagcgc gcagacctca
    19981 cgccagatct ccacgcggtg cgagccgaac gaggcgtccg cctcgccgtg gagcaggcgc
    20041 gaggcttcat acagcgtccc gctctgcccc ggataaaaat acaccgcgag cagcacggtc
    20101 agcgccaaag cgccctccgc cgccagaata tacctccgcc gccgtccgcg tgcgagcttc
    20161 ggcagcatca gcgcgcatga gccgggcagc gcgacccacg cggacgagca ctcgctccgg
    20221 tacaggacat agagcgccat tgccgcgggg agcagcaaaa gaagatcgta cccgcgctct
    20281 ttgaccgccg tatatacgag caccggcacc gcgagagaaa gcacggagcc gaggatcagg
    20341 gtattgccca ccgtgccgag atacatgccg gaatagagcg ttcccgcgtc ggcaaagcgc
    20401 cagccgttcg ggtacagccc gagcgggttc ccgcccgcga tctgccaaag cgccacggcg
    20461 cagcagagaa atacgcttgc tccgaacaca cggacataga tgatcttttt ccgtccccag
    20521 cgggctatgc cgagcgccgt gagcgcatag agaaactgcg tgaaaagccc gtcgtaccgt
    20581 cccgcgccga ggaacgtttt ctcccgccac gggctgagcg ccgcggaaac gagattccag
    20641 acgaggaacg cgcacgttag atagaaaaac gcacccggct tttttacaaa aaggcgcgcg
    20701 ccggtacaga gacacgccgc gatgccgagc gcccagagcg ccgtcacgcc ggtatacagc
    20761 cagaattttg ccgccgtaag gttcgcatag ccgtgaaagc cggtgaagag cggaaagagc
    20821 gccagcaggc agagtatgta cttgtccgtg agccatgcgc agcgcttttc ggcttttgtc
    20881 atgctctgtt cctccatggg atgcactccc tcagtcagca gctttgccgc tgacagctcc
    20941 ctcatggagg gagccggttt taaaaagtat accccatccc ccgcgggaaa gcaacttgcc
    21001 gcggcttctc tcccgtggta gaatgtgccc gtgaggaagg tgcgccctat gatctttgat
    21061 acccacgccc actatgacga caaaaaattc gacgccgacc ggcgggaagt tctcgccgcg
    21121 atgccggcaa agggcgtttc gctcatcgtc gatcccggct gcgacggtga aagctcccgc
    21181 gcggcgtgcg cgctcgcgca ggagtttccg ttcgtatacg ccgccgtggg ctggcacccg
    21241 gaggaatggc agagctggaa cggcgagagc atggcgctgc tgcgctcgct ctgcgcgcag
    21301 ccgaaggtgg tcgccatcgg ggacatcggg ctggactatt actgggacac cgagcacaag
    21361 gcgctgcaga aggagatgtt cgagcggcag ctctcgctcg ccattgagcg taagctgccc
    21421 gtgatcgtgc acgaccgcga ggcccacggc gattgccttg aaatcgtgat gaactacccc
    21481 gaggcgcgcg gcgtgttcca ctgcttttcc ggcagcgcgg aaatggcgaa ggagctcatc
    21541 cggcgcggct ggtatctcgg ctttgacggc cccgtcactt acaaaagcaa caaaaaggcc
    21601 gccgaggtgc tcgccgtgac gccgcttgag cgcattctcg tcgagacgga cagtccgtac
    21661 atggcccccg agccgtgccg cgggaagcgc aacgactcgt cgctgctcgt ctatgtgctg
    21721 gagaagatcg ccgctctcaa gggcgtttcc gcggaggaaa tgacgcgcat cacgcaggaa
    21781 aacggctgcc gccttttcgg tatcgaagca taaaaaaccg ccgtcggagg atcgctcctc
    21841 cggcggcaga ctgttattta tcaatctctg tactttctct gatacatctc cgacggcagc
    21901 agctcgctga gctccgtcag gatcagctcc ggaccggcgg cgatcgggtg ggcgtcacac
    21961 agcggcagct ttcccgtcag cgggagctgc aggtgcatgc tcgtttcgcc gctctcgccg
    22021 ttccggagga caagaacgcc gccgtggagc cgcgcgagcc cctgcgcgat cgtgagaccg
    22081 agccccgcat ccggcgcgcc ggtgagcgta tcctcccgcc gcggcacaaa ggcctcggca
    22141 agctcttcct ccgtcaggcc cggcccgtcg tcgcagacgg tgagctggca gtggcggccg
    22201 aactgtctca gccggaagac gatcttcccg ccgtcgccgg tgcggcggac gcagtcggcg
    22261 atgaggatga gcaggagctg ctcgagcttg ctctgctcgc cgatcacgaa aaggctctcc
    22321 cccgccgggt cgaaccggaa cgtgatgttc cgccgccgca ggaagatctc cgccgagtcc
    22381 accagatcgg cgatgagatc gctgaacgag agaggcttcg gccacgagag cttatcgtcg
    22441 gcggcgagcg cgttgagatc ggcaagctgg ttggtgacat ggaggagctt atagtagctg
    22501 tggtagagga ccgcgctgac tttcgtccgc tcctcgtcat agtcctcgtc ccaggtttcc
    22561 ggctcgtcct tcagcagctg ctcgatcgac agccggagat tgaacgccgc cgagcgcatc
    22621 tgccgcagcg ccgtcggcgg caccggcagt atttccggct cctccgtccg gatcgtgagg
    22681 acaaggatct cgccgctgcg cacggcggta atgtttctcg gtgtatcgcc gatggtgagc
    22741 acggcagcaa agctgtcgcc ccagccgttc tcctccaggt ccgggaagcg ctccgcggcg
    22801 ctctcgccgg taacgtccgt gccgaacagc cggcatgccg ccggattgga gaacacgaag
    22861 cggccctgtg cgatgccgca gacagcgtcc cggctcagcc ggaacagcgg cgcaagcgcc
    22921 gcgccggaga tttccacttt ttccatatca gcagttttcc ctttctgcgc tggattattg
    22981 cccgtatcat accacaaatt ttgcggcgtg gatactacga ttttttgttt tttgcgcagt
    23041 ttcggcgtca gacgctgttt tctatacata ttcactaact aactgtcgag aattatttaa
    23101 tattgtttga aaaaaacact tgttctatgt gcggaaatgt tgtaaaatgc agccggttag
    23161 aaaagagatc aaaaaatttt ttacaggagg atacacacat gattagaatt ggtatcagcg
    23221 gattcggacg catcggccgt ctggcggccc gcgtggctct gcgccgcggc gatatggagg
    23281 tcgttgcaat caacgctccg gacaagactc ctgagcagat cgcttacgtt ttctcccatg
    23341 actccgtcca cggcaagtgg gaaggcaccg tctcctacga cgagcatcac ctgatcgttg
    23401 acggcaagcc catcctgctc ctcagcagcc gcgatccctc caccctcggc tggggcgagc
    23461 tgaacgttcc ttacgttgtt gagtgcaccg gcaagttcct caccgccgag agcgtgcagc
    23521 cccatctgga cgccggcgcg aagttcgtgc tgatgagcgc ccccgcgaag gacaagaccc
    23581 cgatgttcgt ttacggcgtc aaccacaaga cctacaccgc cgatctgaag gtcgcctccg
    23641 ccgcttcctg caccaccaac tgcctcgctc ctatggcgaa ggtcctgcac gacaacttcg
    23701 gcatcgttga aggccttatg accaccgttc acgccgccac cgccacgcag agcatcgttg
    23761 acgagtactc caagaagaac tggcgtctcg gccgcagcac ctacgacaac atcatcccct
    23821 ccaccaccgg cgctgcgaag gccgtcggca aggtcatccc cgagctgcag ggcaagctga
    23881 ccggcatgtc catgcgtatc cccgccccgg acggctccat cgttgacctg accgtgtctc
    23941 tcgccaagga gaccacctac gaggaagtct gcgccgcgat gaaggccgcc tgcgaaggcg
    24001 agctgaagga cgtcatggct tacgaggatc agcccatcgt ctcttccgac atcatcggcg
    24061 agacccactc cagcatcttt gacgcgcagg ccggcatcgc tctgaacggc cacttcatga
    24121 agctctgcgc ctggtacgac aacgagtacg gcttcacctg caacatgatg cgtctgcttg
    24181 ctcacatcgc ctccgtccgc ggcgagtaag tttaagcttc tgcgcgctca atttctcaag
    24241 gcataaactc aatgccccca tcctgcccta agcaggacgg gggcatttct tcgggaggag
    24301 ctttatgtgt cattctaccg tgattctggc atgcagctca cttatggact atgtgaacgc
    24361 agcgcagaaa aaagtcggaa cggaacttcc ggttttgtat atgaatcgcg tctatcaccg
    24421 cgaccccaag gagatgcgcc ggcatattct cgcgcagctt gctgcgctgc cggtcggtgc
    24481 ggataccgtt ctcgtcgcca tggggttttg cggcggttca tgggccgaca ttgaagcgcc
    24541 gtgccgcatt gtgatgccga aaatcgacga ctgcgtttcg ctcctgctgc aatgcggcga
    24601 cacacccgtc agcgatctca aaaagcccgg gcacttgtac gtccgtgaaa aggacccgca
    24661 cagagagtct tttcatgcca tttttgaccg tctgacgcaa gacgttgacg ccgaaacaaa
    24721 aaagcgttat cacgaggact ggaagcggct ctattccaac atcgacatta tggacaccgg
    24781 tctgaaccgc tgccgcgaag cggcataccg cgccatagtg caggaagatg ccgattggtt
    24841 ggatgcggag ctgtcgtatg tttccgccgg aacgcacctg ctggaaaaac ttttttccgg
    24901 ccaatgggat aggcagtttc tggtagtaga aaaaggcggc tgtgtcaaaa aagagcagat
    24961 cctgatctaa acgttccccg gaggatttta tatgaatacg gtacttctcg gcaacatcgt
    25021 tgcactcatc ggcgcggcca tcatggtctc gaccggcttt ctgaaaacaa aaaagcagat
    25081 gctcaccgtg cagtgttttc agttcggcgt gctgagcgca tcgaacgcta ttctcggcgc
    25141 gttcacgggc atcaccgcca acgtcatcgg catcgcgcgc aatctcttct gcatgaaaaa
    25201 agagttcacg acgccgtgga agatcgtgtt catcgctgcg cagcttgcgc tgctgttcgc
    25261 cttcaactcc gacggctggt tcgggctgct gcccgcggcg gccacctgtg tgtacacatg
    25321 gtttctcgat ctcaaggacg cggtgcagct caaaatcgtc attatcgtca cgctgctctg
    25381 ctgggcggtc tatgacttcg ttttcaaaaa ctatgtgacg ttcgcctttg atttcggcgc
    25441 ggtcgtgtcc aatctcatcg gcatcgtcac gctgcggaaa gcggcaaagc cggagtaatt
    25501 ttctcccccc ttaagcgctt gaaccggact gtgattccca aagccttttt ctgccttcaa
    25561 acaagcagaa aaaggttttt ttcgcttaac agaagacggc agttctcgcg ggcttttgtt
    25621 tccgctttac ctgtttttta cgcagttcag gtttcgcagt ttacattccg cctttatact
    25681 gaattatgag cgcaaaaaga aagccgctgc cgtacaagaa gacgctaaag cacatacaac
    25741 ggttaaaaag gagcaataga atacctttct tcggtatttc ggcgcaaaca ctatgtgata
    25801 tttgcaattc cacttcgtat gagataaact gtttatg
//
LOCUS       DAWAAE010000014        25740 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_826_length_25740_cov_2.811369, whole genome shotgun sequence.
ACCESSION   DAWAAE010000014 DAWAAE010000000
VERSION     DAWAAE010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 25740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25740
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_826_length_25740_cov_2.811369"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    283..292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1854..1863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11773..11782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12611..12624
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16031..16145
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5284 a         7635 c         7710 g         4952 t
ORIGIN      
        1 gacgaggaag aggccgtgga agcgctcacc gcgctgatcg cctcggactt cgcggagtga
       61 tctgcggaag caaacaaacg gacaagggcg tgttataaac acgcccttgc ttttgcattc
      121 tgggactttt cggaggatac cgtgaccaag atcgaggagc tgcgcgagaa agccaaccgc
      181 ctgccgcttc tgccgggcgt ctatattatg ctggacgctt ccggcgaggt gatctacgtc
      241 ggcaaggcta aggcactcaa aaaccgcgtg acgagctatt tcnnnnnnnn nngtcttgac
      301 cggcgggagc cgtacccgcg ctttacgctc tcaaaccgcg cgaacccgga cggggcaaaa
      361 tactttggcc cctacggcgg ccggggacag acgatgagca tcatcgatac gctctgcaaa
      421 acgctgggcc tcccgacctg ccgccgcgtg ttcccgcgcg acatcggcaa ggaccggccc
      481 tgcctgcagt accagatggg cgcgtgccgc ggctggtgcc gcggcgagcc gggcggggaa
      541 gagtaccgcc gcgcgatcgg cgaggccgtg gatatccttg aagggcgcag cgcggagctc
      601 gtccggcgca tcgagaccga gatgaatgcc gcggccgaag acctccggtt tgaagaagcc
      661 gggcgcctgc gcgaccggat gcgcgccgta tcaacgctgg aaaaccgcca gcgcgtcatt
      721 gccgctgccc gcgccgatac ggacgccgtc ggtttcgtgc gcggcgcgaa aagctgcttt
      781 tccgtgctgc actatacgaa cggcgacctc tccggcaagg atgtatcgct cattgacgat
      841 ccgacggaaa cggacgggga ggctctgtcc accctgctgc gccaatatta tgttaaccgt
      901 tcaagctggc cgaaaacgat ccttctgccg tttgagccgg aggataaaga ggacattgag
      961 cgctggcttt cggatatggc cgggcggcgc gtgtatctcg aggtgccgca gcgcggcgac
     1021 cgcgcccgct ttgcggaaaa ggcggcagtg aacgcccgcg aggagatatt gcgctccacg
     1081 accgccgagc agcgccggag caaaacgctc tcctggcttc aggaagcgct tcagctcacg
     1141 gaatttccgc accggatcga ggcctatgat atctccaaca ccggcagcac gggcatcgtc
     1201 gcttccatga cggtgcatgt ggacggcaag cccctcaagc gcgattaccg gaaattccgc
     1261 atccgcagca ccgaaacgcc ggacgactac ggctccatgc gcgaagtcat cacgcggcgg
     1321 ttcacgcact atctggatga ggacggaaag ttctccgcaa ggccggacgt tttgttcatc
     1381 gacggcggcg cggagcacgc ttcggcggcc tgcacggcgc tcgaagcgct gggactcagc
     1441 ctgccggtgt tcggcatggt gaaggacgac aggcaccgta cgcgcgcgct cataacgcca
     1501 gagggacacg agatcggcat acaggcaaat cccgccgcgt tcgcgcttgt aggccgcatt
     1561 caggaggaaa cgcaccgctt cgccatcgag tatcaccgct cgctgcgcga cagcgtgcgc
     1621 ggctccacac tcgataagat ccccggcgtc ggggaaaaac ggagaaacga tcttttgaag
     1681 accttcggct cggtgcgcgc gatccgcgcg gcgagcgcgg aggagcttgc ccgggccgtg
     1741 ccgaaaaaca cggcgcaggc tgtatacgac tattttcaca aaaccgagga ggaaacacca
     1801 tgcgagtgat caccggcaca tgccgcggcc gccgtctgct cgagccggtg gggnnnnnnn
     1861 nnncgatcag gtgaaggagg ccatgttcaa tatcatccag tttgatgtgg aggggcgcaa
     1921 agtcctcgat ctgttcgccg gaacgggtca gctcggcgtt gaagcgctct cccgcggcgc
     1981 ggcggattgt ctgtttgtgg acagcgccag ggactccgtc cgcctcgtgc aggaaaatct
     2041 ccgccgctgc ggtctcgcgg cgcgcgtgct gcagtgcgac gcgctcggca ttctgaaatc
     2101 cggcgagaaa ttcgatctca ttttcctcga tccgccctac ggctccgggc tggaagcaaa
     2161 agcgatcgag acggtgaaag agtttgacat cctctcgcgc ggtggtataa tggtggtcga
     2221 gacccggccg gaaacggcgc tgccggtcct tccgccggaa tacggtacga cgaagacgta
     2281 ccgttacggg aaaatcaaac tcacggtcat aaccagagga aacaactgat gcagacagcc
     2341 atttgccccg gcagctttga tccgatcacg ctcggccacc ttgatgtgat ccgccgcgcg
     2401 tccaaaatgt tcgagcgcgt tgtggtctgc gtggtggcga accccggaaa acactgtgcc
     2461 attacgctcg aagagcgctg cgagcaggtg cggcgcgtca cggcgtccat tcccaatgtc
     2521 gaggtcgata tgtgggaagg tctgctcgtg gactacgccg aacgatacga aaaccccgtc
     2581 gtcgtgcgcg gactgcgcgc gctgtccgac tttgaatacg aatttatgat ggcgctcacg
     2641 aacaaaaagc tcaactcccg tgtggagacg gtgtttctgg cgtcggacga aagatacatg
     2701 tatctctcct cgaccaccgt gcgcgagatc gcaagctacg gcggcgatat ttcccgcttt
     2761 gtccccgcag agatcctcga cgacctgatg ataaaactca acgggaggaa ataaatatat
     2821 ggaaaaccac gcgctggata tcatcgatat gctctataat atgattgcgg aggcatgggg
     2881 cgtccctctg ggcaacgaca agtgcatcgt tgagcgcgac aaggctctca atcttctcga
     2941 cgatcttaag gccacgctcc cgatggagct gagcgaggcg cgccgtctcg tcaacgcgcg
     3001 cgacgaattc gtcgccaacg ccaagcagga ggcggacaac atccgccgcg gcgccgagga
     3061 taaggccacg cagatgatcg acgagcagag cgtctatcag gaagccaagc gccgctccga
     3121 cgagatcgtc caaacggcgg aaacaaagtc ccgcgagctg ctgcgcgcgg cgaacagcta
     3181 tctggacgac gccctccgcc agacggagga ggccgttgcc gcgtcgctcg aaaaggtccg
     3241 cgtggcgcgc gcgcagttcc gcgccgccgc tgcgcccgag cagccggaag gcaccgcgga
     3301 cgtcacgctg caggatgtgg aggacagcga gtttatcaac ggctgagtcc tgcggctgaa
     3361 cggacgaaat tctcctgaaa agcgaatttt gtctgcccaa tcgtaaccat ttgtcaataa
     3421 cgagacggaa gccgccggag cgcaccatat actgcgctcc ggcggctctt tttgcgtttt
     3481 ttatacaaca tatgggccgt tcgccacgga agatgcactg ttccaacgga attttgctct
     3541 tgcatttttg ccccctgcct tttataataa aacagcatag tggggcaaag tggggcaaag
     3601 tgtcacggtt gtccacgtgc gaacagagaa gggaggcgtc gcgtatgacc ggcgaatata
     3661 tgcacacgct cgatgccaag gggcgtcttg cggtgccatc gcgcctgcgg gacgagctcg
     3721 gcggcgtttt ctatgtgacg ctctcgatgg atcgctgcct ttcggcgtac agcgccgaga
     3781 gctggcagcg cttttccgat aaggtggacg cgatgccgtt cgtaaagcag cgcaggatgc
     3841 gcccgctctt tgccttcgcc gcgcgctgcg aggcggacgc gcagggccgc attctcctgc
     3901 cgcagaatct gcgggactat gccggcctca cgaaggacgt gaccatcgtc ggctgcaaca
     3961 accatgcgga gctctgggac agcgcgcgct ggaacgagat ccacgatgcg gaaatgacgc
     4021 cggagaacat tgccgccgtc atggaagagc tggagttttg atatgccgga acacaaaagc
     4081 gtgcttctgc acgaatgcat tgaaaatctg aatataaagc cggacgggat ctatgtggac
     4141 gggacgctcg gtctcggcgg ccactcgatg gagatcgcca agcgactcac gaccggacgg
     4201 ctcatcgcca tcgatcagga tgagaccgcc atcgagcggt caaaggcgcg tctcgcgccg
     4261 tgggcggagc gcatcacatt cgtccacgga aatttccgct cgctcggaaa gatcctcgac
     4321 gatctcggca tagaccgggt gaacggaatg ctctttgatc tcggcgtatc ctcgccgcag
     4381 ctcgacgaag ccgaacgcgg cttttcctat atggcggacg cgccgctcga catgcgcatg
     4441 gatcagagcg caccggtgac ggcgtggacg gtcgtgaaca catggccgga gcgggagctc
     4501 gtgcgcatcc tgcgcgacta cggcgaggag cgctacgcca accgcattgc gggaaacatt
     4561 gtccgcgcgc gggagaagaa gcccgtggaa acgacgctgg aacttgtcga tatcatcaaa
     4621 agctccatgc ccggcgcggc gctgcgcgaa aagcagcacc ccgccaagcg cagctttcag
     4681 gcgatccgta tcgccgtgaa cgacgaactg gaggcggagc gggaaatgct gctcacagcg
     4741 atggatcggc tggcgcccgg cggaaggctg tgcgtgatca gcttccactc cctcgaggac
     4801 cggatcgtca agaccgccat ccgcgagcgc gaggacggct gcacctgccc gcgcgatttc
     4861 ccggtgtgcg tgtgcggctt tgtgcagacg atgaagaccg taacgaagcc catcacagct
     4921 tcggaagagg aactgaacga caacccgcgc gcgcgcagcg cgagactgcg tgttgccgag
     4981 agaatataaa aggggagata cccgtgaacg caaaaacgac cgaacagaaa accgcggtat
     5041 tcaccccctg cgcagagagc gcggaagcaa cgcgcgcttt ccccgttctg gatgaggata
     5101 ccagagagct gcccctgatc gccgaccggt ttgccgggcg gtactgggac ggacgcatgg
     5161 agaacgagac ggaagagaca ggcgagaaac cggcgcggaa aaagacgtgg cgcagcgcgc
     5221 ttgcctttgg cgccgccgcg gtcgcggcca tgctgcttct tgtcggctcg ctgaccggac
     5281 aggcggagct taccgcgatg aatgatgagc tcgtctccgt gtcggcgcgt atctcggagc
     5341 tcaaagcgga ggagcgcacg ctccttgtgg agaccgaaaa agccgagagt ctcgccgccg
     5401 tggagagcta cgccgtggga acgctcggca tggagctgcc ccggctcgat cagcgcaata
     5461 ccggcgcatc caagctttcc gaccgggcca cggtgctcca cgcgcgccgc ggacagggcc
     5521 tgcggtattt ctgggagaac cttctcgaca cggttatggc atgtttttga tcccggcgcg
     5581 gtataatgca tcagaatgat gcatttgccg atccggagga aacgccatgc cgcccaacag
     5641 ccgacccaac cgaaccatgc tccgccgcgc gctgttcctt cttgccgcgt gcggcattgc
     5701 ggcgtttgcc gtattgatgc tccagctttt ccggctgcag atcctgcgcc acgacgagct
     5761 ggagagcgcc gccttgcagc agcagctccg ccgcacggcg atccccgcct cgcgcgggac
     5821 gatctatgac cgcgagggac gcgtgcttgc aatgagcgcg acaacgtata ccgtgtacgt
     5881 gagccccgcg gagatatcca tgaacggcga ggacgccgag cgcattgcgt ccggtctttc
     5941 cgagatcctc ggcgtagacc gcgaaaagat cctcacgatg accgccgacc ggcgcagctg
     6001 gtacaaaacc gtcaagcgcc agatcgagga agccgaggcg gacgcggtgc gcgcctttaa
     6061 aaacgagtac gacctgcagg gcgtgaagct cgagccggat accaagcgct attacccgta
     6121 tgcttcgctc gcctcgcatg tcatcggctt cgtcggcgcg gacaataccg gtctttcggg
     6181 actggagttt acgcagaacg agctcctgac cggaaccgcc ggaagcatcc tccggctgaa
     6241 gaacagcgcc ggaacggata tgatcctcac aagctatgag gattacgtcg atgcgcagga
     6301 cggactcaat ctccacttga caatcgatac caccgtccag tactatctgg agaagcagct
     6361 ttcccaggcg gtgcaggact ttgatataca aaacggcgcc gccggcatca tcatggaccc
     6421 gaatacgggc gcaattttgg cgatggcgtc gctcggaaac tttgatctca acaactacca
     6481 gcgcgtctcc gccgacatcc aatcggagat cgattcgacc gccgatccgg acaccgcggc
     6541 gcagatgctg aaagacgcgc agcttgccca gtggcgcaac aaggccattt ccgataccta
     6601 cgagccgggc tccacattta agatcatcac gcttgccatc gcgctcgaag agggcgctgc
     6661 catgccgtcc gacagctttt actgcggcgg cagcatgacg gtgcagggac gcggcaagcc
     6721 gcttaagtgc tggaaaacga tcggccacgg tcctcagacg cttacgcagg cggcgcagca
     6781 ctcgtgcaac gtggcgtttg ccacgctggg acttcgcatc ggcgaggaaa cgttctaccg
     6841 ttactgcgag agcttcggct ttttctgcgc gagcgaaaat ccggacgctt ctctcacggg
     6901 gaagaccggc atttcgcttc cgggcgagag cgggagcatc tggtggccgc gcagcgtttt
     6961 ctgcaacagc gaaaactttt cgcagcttgc ggcggcgtcg ttcggccaga cgttcaacat
     7021 cacgccgctc cagctcatca ccgctgtgag cgcctgctgc aacggcgggt atcttatgaa
     7081 gccgtacatc gtcgattccg tcacggacgg caccggcgtg cttatatcga aaaccgagcc
     7141 ggaggtgctc cggcaggtca tttccgagaa gacgagcgcg caggtgaacg ccgttctgga
     7201 gcaggtcgtg tgcgatacga agcagggcac cggcaaaaac gcctatgttg ccgggtatca
     7261 catcgccggc aagaccggca cctccgaaaa ggtggcgcag gacgccgccg gcggaaagaa
     7321 ggaatacatc gtctccttca tcggctacgc gcccgcggac gatccgcagg tcgtgtgcct
     7381 tgttttgatg gatacgccct caaacgagag cggtatctat atctccggcg ggcagatggc
     7441 cgcgcccgtc gtcgggcgca tcctcggcga agtgctgccg tatctcggcg tacagccgca
     7501 atacagcgaa gcggaggaaa aatacatcga ccgcgctgtg ccgccgctca ccggcaaaac
     7561 gccggaggaa gcggtaaagc tgctccgcga ggccgggctc gccgcccgga tcgaagggac
     7621 cggcgacatt gtcaccggcc agctgccggg gaagggaacg gtcgttgcga gcggcacaac
     7681 ggtgctcatc tataccggtg aaataccgga ggcagaagag gagaccgtgc cggagctcac
     7741 ggggctcacc tataccgagg cgcgggaagc gctcttttca cgagggctgt tcctccgctc
     7801 ggacagcacg atcctcgccg acagcgatac ggtgcgcgtt gtgttccaga gcaccggcgc
     7861 gggggagagc gttcccacgg gcagcgtaat tgaggtcagc atcgtcaacg acgataacga
     7921 tacatacggc cggtattgaa aagccggccg ggacaatgag gaagtaccat gaaactatcg
     7981 gaaattctca aggaaataaa cgttctctcc gcagccgcct ccggcgatac ggagatcacg
     8041 ggcgtctgct acgattcccg ccgcgtgaag agcggtgacc tattcgtcgc cgtgcgcggg
     8101 tacgagtcgg acggacataa atatatcccc atggcggtgg aaaagggctg cgccgccgtg
     8161 ctgtgcgagg aagcgcccgc cggaaacgtg ccgtacgtcc agacggacga cagccgtctc
     8221 ggtctcgcgc ttgccgcgcg gaacttctac ggcgacccca gccgcgaaat gaaggtcatc
     8281 ggcatcaccg gcacaaacgg caaaacgacg accaccatcc tcataaagca gatcctggaa
     8341 aagaccctcg gcgcgaaggt cgggctcatc ggcagcatct cgaacatgat cggcgacgag
     8401 gaattccacg ccgagcacac cacaccggaa tcctgcgacc tgcaggcgct cttccgccag
     8461 atggcggatg ccgggtgcac ccactgcgtg atggaggttt cctctcactc gctggtgctg
     8521 cgccgtgtgg cgggcgtgca ttttgccgtc ggcctcttca cgaatctgac gcaggatcat
     8581 ctcgacttcc acaacacgat ggaggaatat gcgaaggcta aggcaatgct cttccccatg
     8641 tgcgaaaaga cggcggcgaa cgcggacgat ccgtggaccg aaacggttat gaaggacgcg
     8701 ccgcagccgg tgtatacctt ctctgcgaag agcgacagcg cttcgctcac ggcgcgcgat
     8761 atccgtctcg cgccgagcgg cgtgcgtttc tgcgcgctgg aagagggcga gctcgtccgc
     8821 gtaaagacga acctgccggg acagttttcc gtgtataacg cgctcgcggc gatctcgtgc
     8881 tcgcggctgc tcggcgtatc cctcgcggac gccgccgcgg cgctcgaaac ggcccacggc
     8941 gcaaagggcc gcgtagaacc ggtgccgacg gacggcgact acagcatttt catcgactat
     9001 gccgtcacgc cggatgctat tgaaaacgtt ctcacgacgc tgcgcggcat tgcgccgaag
     9061 cgtctcgtga tgctcttcgg cgccggcggc gaccgcgacc ggaaaaaacg cccgatcatg
     9121 ggaaagctct ctgcggacat tgccgatttc gtcatcgtta cgagcgacaa cccgcgcacc
     9181 gaggatccca tgagcatcat cgaggagatc ctgcccggcg tgcgcgcggg caaagcgccc
     9241 tacaaggtca tctgtgaccg cccagaggcc atttgctggg cgatcgacca tcatgagccg
     9301 ggcgacgtca tcctgctctg cggcaagggc cacgaggatt atcagattat cggccacgag
     9361 aagatccaca tggacgagcg cgagatcgtc gcggactatc ttgccgaacg cgcgaagcgc
     9421 acctgacaaa gcaaaagagg aaaggcacgg aaaaaagaat gacttggaag ctgattttgg
     9481 cgcttgtcgt cggattttgt atatcgagcg ccgtaggcgc ggttttggta ccgtatctgc
     9541 ggaaaatcaa agccgggcag gcgatccggg aagacggccc cacgtggcac atgagcaaga
     9601 ccggcacgcc cacgatgggc gggctgatgt tcatcatcgc cgttacggtc gtttgtctca
     9661 cggtcggctt ctcctgcatg gcgaacggcg agtggggaca cattttcgta ttggtgttcg
     9721 gtctcgtgtt cggcgcgatc ggctttctgg acgactatga aaagctgcgc cacaagcaga
     9781 atctcggcct gagcgcaaag atgaagttcc ttctgcagct ccttgccgcc acgtgctttt
     9841 tgctgctgct ccgcttcacc ggctatctga gccccaatct ttatattccg ttctggaaca
     9901 cctatgtccc ggtctccgag ccgctgtact tcatttttgc ggcgtttgtc atcgtcggca
     9961 cggtgaacgc cgtgaacatc accgacggcg tagacggcct cgcggcgggc acctgcgtgc
    10021 ccgtgttcct ctgcttcacg gccatcgtgt tctgctgggg caaagagtat atgccgcagg
    10081 gcattttcgc cagcggcatg gtcggcgcgc tgctcggctt cctcatctac aatttcaacc
    10141 ccgcgcgcgt attcatgggc gacaccggct cgctcttcct cggcgggtgc atctcgggtc
    10201 tggcgttcgc gctggatgtg ccgctgctgc tcatcccgct ttgcatcatg ccgatcattg
    10261 aaacgctttc ggacatcatc caggtggcgt atttcaagct ctcccacggc aagcgcgtat
    10321 tcaaaatggc ccccttccac caccatctgg agatgggcgg ctggacgggc cggaaatgga
    10381 aagaaaaaga gatcttcgtc ctgtttgcgg gcatcacgct cgtgatggcg gtcgtatcgc
    10441 ttctcggcgt aacgggacgc tattccttct gagcttttga gagggtaata ccggaggctc
    10501 tcatggatca tacttcgacc ggcgccgcgc cggtcaaaac gggtaaggca gaccgcaggg
    10561 cggatctgcc tttccttgtt atcacgctca cgctgctcgg cgtcggcgtg gtgatggtgc
    10621 tctcggcgag ctttgcgagc gcgtattacg atctgcaggg cgagaccgga cacaacccgg
    10681 tgtatttttt ctcccggcag gccatcttcg ccgcggcggg cgtgtgtgta atgctcatcg
    10741 cctcgcacat tccgatgcgc gtttatcgcg cctgcgcgct gccgctgctg ctcgtttctg
    10801 tcgcgagcct tgcggctgtg ctggtcatcg gcacggtcgt aaacggcgca agacgatgga
    10861 ttagccttgg cttcacgacg ttccagccgt cagaaattac aaaaatcgca attattcttt
    10921 acttttcggc gctgatatgc aaatataaag accggatgcg caccttccgg tacggcatcg
    10981 cgccgttcgc ggcgatactg gcggtcgttt cggcgctgct cgtgcttgag ccgcactttt
    11041 ccgcaacgat catcatcctt gccatcggag cttccatgct ctttctcggc ggggtacggc
    11101 tgcactggtt catcggcgcg gccggtgtgc tgctcgtcgg cgcggtcgcg gcggtgctgt
    11161 tcgtgccgta tgtcgcggcg cgcgtggcgt ccttccgcga ccccttctcc gatctcaccg
    11221 gcgacggctg gcagatcgtc cagtcgctct atgcgatcgg ctcgggcggg ctgtccggtc
    11281 tcggtctcgg catgagccgc cagaaatacc tctatcttcc cgaggaacac aacgacttta
    11341 ttttctccgt cgtgtgcgag gagctggggt tcatcggcgc ctcggcgatc cttctccttt
    11401 tcaccatgct catcgtgcgc gggtactgga tctccctgca gagcaccgac cgcttcagct
    11461 tccttaccgg ctgcggcatc acaacgatgc ttgcgctgca aatcgtgctg aatgttgcgg
    11521 tatgtacgaa tctcgtcccc tgcacgggca tctcgctgcc gtttttcagc tacggcggta
    11581 cggcactgct catccagatg ggggagacag ggatactgct gagcatatcg cgggagaacc
    11641 cctgtgcggc ctacgcgccg cgggaactct ttaaaaccgt aaaaaatcaa aaaatcgagg
    11701 aatgacctat gcgttttctg tttgcctgcg gcggcaccgc gggccatgta aatcccgccg
    11761 tcggcgtggc cgnnnnnnnn nngccgggcg gatccacgag cttctgccgg acagcgaaat
    11821 tttgttcatc ggcgctctcg gcaaaatgga aatggagctt gtcccccgcg aggggtacga
    11881 gatccgcggc gtgccggtga gcaatctgag ccggtcgctc tcggtggagg gcatcaggca
    11941 caatataaaa tttgtctccg atctcatcaa gtccggcccg gcgtgccgga agatcatcga
    12001 ggaatttaag ccggacgtcg ttatcggcac cggcgggtac gtctgctacc cggtgctgca
    12061 tgaggcggca aagctcggca tccccaccgt cgtgcacgag agcaacgccg aacccggcct
    12121 tacgacacgc cagctggaaa agtgcgtgga taagatcatg gtcggctttg ccgaggcgcg
    12181 ggagaactat aaaccccccg aaaaggtcgt cgtgaccggc acgcccgtgc gtctcggctt
    12241 catgcgctcg gataagagcg ccgcgcgccg tgcgctcggt atcgcggagg acgaaccgct
    12301 cgtcgtttcc gtttggggca gtctcggcgc aacggaaatg aacaagaccg tcgcgcgcat
    12361 gatcgctctc gccatggacg gcgcggagcg ccccttccgg ctcgtccact cggcgggcaa
    12421 gcgcggcata aagagcatga cggagtatat gagcgaagag ctcggcctct ccgactggaa
    12481 ggaagccggg tttgacgtgg tggactatat ttatgatatg ccccttctca tggcagcggc
    12541 ggacatcgtc ctctgccgga gcggcgcgtc cacgcttgcg gagctcaccg ccatcggcaa
    12601 accggcgatc nnnnnnnnnn nnnnggaaaa gctcgccgaa atggaagagc atatgcgcga
    12661 gcttgccgtg gagaacgcca ccgacgagat cacatctatt gtcctctctc ttgcctccgg
    12721 ggagtaattt ggcaaacggc gcttcctctg catagtatgg cttgaaaatg ctgtgcgggg
    12781 gaagaactat gagtatatgg catataaccg gcggatcgcc gcttcgcgga cagatccgcg
    12841 tacagggctc aaagaacgcg gttttgccgg tgatcgcggc gtcgcttctc tgcggcgcgg
    12901 aaacggagct gctcaactgc ccgaatctga gcgatgttga cgcggcgatg gacattctcc
    12961 gctatctcgg ctgcaccgcg gcgcgctgcg gcgacgctct ctgcatcgat acgaccgcgc
    13021 ccgcgcggtg cgatattccg cgcgagctga tgcaccggat gcgctcctcc gtgatatttc
    13081 tcggcccgct gctcgcccgc ttcggcgagg cggaggtatc gctccccggc ggctgcgagc
    13141 tcggcccgcg gccgatcgat ctccatctgc acgccctgag gcttctgggc gcggagatcg
    13201 aggaagggga ggacagcatc gtgtgccggg cttcgcacct tcgcggcgcg gagatatcgc
    13261 ttgcgctgcc aagcgtcggc gcaacggaaa acgccatgat cgccgcctgc gcggcggagg
    13321 ggacgaccgt catctataac gccgcccgcg agccggagat cgaaacgctg caaagctatc
    13381 tttgcgcgct cggcgcggac gtatccggcg caggctcgcc tgtcatccgg atcaccggct
    13441 tttccccgcg ctctcacgcg ggacttcgca tcccgcccga ccggatcgcc gcggcaacgt
    13501 atctctgctg cacggcgtgc gccggcgggg agatcgagct taccgccgcc gcgcccggac
    13561 agatcgtccc cgtgcttgac gcgcttcgcg cgatgggctg tgccgtgagc acgcacggag
    13621 atcgtataca tatgaccgcc cacggccgcc cggcctcgcc cggcgcgatc gtcacaaggc
    13681 cctatcccgg cttcccgacg gacgcagcgc cgctgctgat ggcggcgtcg ctccggtgca
    13741 caagaccggc gatgtttatt gagaacattt tctccggccg ctaccgccac gccgaggaga
    13801 tgcgcgctct cggcgcgcag atcgtcctgc gcgggccgat ggcgcttgta accggcgtgg
    13861 aaacgctgca tggcgcttcc atgaaaagcc ccgaccttcg cggcggagcg gcgctcgtgg
    13921 ccgcggcgct cggcgcggaa ggggaaagta ccgtgaccga cgccggtcac atcctgcgcg
    13981 gctacgaatc gctggacgag acgctgcgct cgctcggagc atccgtctgg cgcgagaaag
    14041 catagagcaa cactacacaa aaggagcaga acatggcatc ggaaatggaa ttcagtcagc
    14101 acaataaagt aaagcgccgc agcccgtggc atgggccgat ggtcctcgtt ttgcttttct
    14161 ttgtcgttat tctggttatg agcttggcgt tccgcgtctc gaagatcgag gtcatcaacg
    14221 cctcggagta taccgacgag gagatcatca acgcctccgg cgttgagcgc ggcgcgaacc
    14281 tcttttttgt ggaccgcttc aaggcggcga gcatgatctt ttccgacctg ccgtatatgg
    14341 acaccgtgag catcacgcgg cagctcccga acaagatcgt cattcaggcg gagggctcgg
    14401 cgcccgcggc gtatatggtc attgacgagg agtactggct gctcgaccgc agcggaaaaa
    14461 tgctcggcac gaccgattcc ctcacggcgg ggaagtatcc ggagatccgt tcgctcgaac
    14521 cgatgacggt gcttgagggg gaggatatgc ttgtggaggg taccaatgcc gaacgccttg
    14581 ccgtgctgca gacgatcctc ccggcgctcc gcgcggagga cgtttcctcc ctcgtggaat
    14641 ggatcgatct ccgcgatccc gcaaacccct cgctgcgcta cgacggccgt ctcacggtct
    14701 atctcggcgc ggagggtgag atgctctacc gcatcgccat gctcaaaaac gtcgtttccc
    14761 agctcgccgc ggaggacgcc ggaacgctct attatacgaa cggcagcgcc tggactttca
    14821 gcccggattg agcagaatgg cggttttctt ttcaaatttg ttacaaaaac cggccgcagg
    14881 tcaacataat gctgttgacc tgcggttaaa aacgttataa aatgacaaga gtatgagtat
    14941 aaaccccaaa taggaaccgg gagggtatat agaatggcaa tcaccaaaga cgcggagaac
    15001 gttgtcacca tcaaagtcat cggcatcggc ggcgccggca acaatgttat caaccgcatg
    15061 gcgtccgttc tcctgggagg gccgccgccg cgcgctgcag gcgcagcagg gcgtcagcga
    15121 gctgatgggc cgcgtggaca gcctcctcat catcccgaac gaccgtctga aatacgccag
    15181 ccccgagaag gtcacgctgg ccaacgcctt cgagatcgcg gacgacgtgc tcatcaaggc
    15241 cgtgagcagc atttccgagc tcatcaagaa caccggcttc atcaacctcg actttgccga
    15301 cgtgaccgcc gttatgaagg gcgccggtct cgcgcacatg ggcgtgggcc gtgccgccgg
    15361 caagaacaag gccgaggatg ccgcgaagat ggccgtttcc agcccgctga tggagacctc
    15421 tatcgacggc gcgcgcggcg tgctcatcaa catcaccggc tccaaggaaa tgggtctcga
    15481 cgatgtcgag gcggccgcca tgctcgtgca gggcgcggcg catccggaag ccaacatcat
    15541 cttcggcgcg agctttgacg attctctcga tgacgagatc gtcgttaccg tcatcgccac
    15601 cggctttgac gagaaaaagc ccgagaccgt caagatcgag gagccgcgcg acgcgaacct
    15661 cttcaccgac gcgcgcgagc gcgccgagag ccagaaggag cccgcggcgg tccccgcccc
    15721 ggaagaggac ggcgaggatc cgttcgatac gatcttcaag atcttcaaca agtaatttca
    15781 tacgaaaaag catccggcga gatttctctc cggatgcttt tttcattccg ccgggagcgc
    15841 cgcgatatcc gaaacggaaa tttcgtacgc cgtccggcgc actgcctgcc cgtcgagatt
    15901 ttttatgtac tcgcggctct ggatgcgtcc gagcagatgt acgcgcgtac cgacatccca
    15961 gagcgaggcc cggcgcgcgt tctgccccca gacgatgcag ggaagatagt cgcttcgccc
    16021 gtagagccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnagcgt gcccgtcagc tcgacctcgt tgacccatac gggcgtctcc ggcggagaca
    16201 gctcacgggc gaacacactg atgacgagct tgctgccctc gccgctgtga ttgttgaacg
    16261 agcgcacctc gcccgcaatg cggatgagcc ccgcgcgtga cggcttgagc tccggcagaa
    16321 gctcctcgcg gagaagaacg ttgatcgtgt ccaccgttcc cgagagccgc gcgacctcca
    16381 gcggaaacgt gtagaacggt tcgagacggc tggcgtggga aaaaaccggg gaagaaagcg
    16441 gcgtgccgca gagaatgacg cgattgtggg tttgcatttc atccatttgt aacacctcgc
    16501 catagcctat tcactcgccg gggacaaaat gccttgtttc tttgacgcgc gtgtgatatg
    16561 atgcagacat caataagaag gaaaggcaga acatcatgga tataaaagac attcttgccc
    16621 gctgcgacca tacactcctc cgcaccgact gcaccgccgc ggagatccgc gctctgtgcg
    16681 atgacgcgat ccgcttcggc tgcgcctccg tgtgtatccc gcccgcgcat gtggcgggag
    16741 ccaagcggta tgtaaacgga cagatgaaga tctgcaccgt catcggcttt ccgaacggct
    16801 acaacaccac cgccgccaag gtctttgaga ccgaggacgc cgtgaaaaac ggcgcggacg
    16861 agatcgatat ggtcatcaat ctcggcatgg tgaaggacaa gtgctgggac atgctgcttg
    16921 acgagatcgt ccgcatcaag gctgcgtgcg gcggaaagct cctcaaggtc atcatcgagt
    16981 gctgcctgct cacggacgag gaaaagatca agctctgcga gatcgtctcg gcgagcggcg
    17041 cggagtatat caagacctcc accggcttct cgaagggcgg cgcgacgttt gacgacgtgg
    17101 cgctcatgcg cgcgcactgc gctccctccg tcaaggtcaa ggccgcaggc ggcatctcgt
    17161 ccgtcgagga tgccgaacac ttcatcgagc tcggcgcgga gcgcctcggc acgagccgca
    17221 tcgtgaagat cgccaagcag ctcgaagcgg aaaaaacgga ataaaaaaga aacgggaaag
    17281 aaacgattct ttcccgtttc agtctgtcga aaaagtccgg cttcggctgg gctttttcta
    17341 caggctgaga atgctttgtg tattctcaag ggctttgacg cggtatgagg gcatttccgc
    17401 ccggcagaac cgcgtgtgcc tttgtcgatc gacgcaaatc agagtaaatg taataattat
    17461 aagccgacag gcaaaggaac agaagccata aagctgcgcc gatccacgat atgatttttt
    17521 tcattggaat ccttcatctt tccgttcatg ctttatcttg ccgcatattc ctgcagatag
    17581 cgggcgcgtg ccgggtgacg gagcttgcga agcgctttcg cttcaatttg acggatacgc
    17641 tcacgcgtaa cgagaaacga ttcgccgact tcctccagcg tccgctcgcg cccgtcaaaa
    17701 agaccgaagc gcatgcagat gacagtgcgt tcccgttccg taagtgttga gagcgctttt
    17761 ctcatatctt cgctcaatgc gttcatgagt gtcctggatt cgggggcggc atcttcgctg
    17821 acggcgagaa aatcgccgac ggtgctttca ccgtcctcac cgacaggcgc atcgagggat
    17881 accgtgtcag agatcagctg cgaaagcgaa cgaacgcgtt cggtatcgca gccgatgtta
    17941 gcggcgagct cctcaacggt cggctcacgt ccaagttcaa gcgtgagccg ctggagtgcc
    18001 gtgcgatatc ggttcagcgt ttcatgcata tgtaccggaa gccggattgt acggctctgg
    18061 tcagcgatcg ctcgcgtaat ggattggcgt atccaccacg ttgcataagt cgaaaatttg
    18121 aagccgagat gggggtcaaa cttctgcacg gcccgcatca aaccgagatt cccctcctgg
    18181 ataagatcag agagtgggag accgcgattt acatagcgct tggcgatcga gacggacagc
    18241 cgcagatttg cctcgatcat cctgttccgc gccgcctcgc tgcccacggc ggccgctgca
    18301 agctcaactt cttcttccgc tgtcagcagc gcatgccttc cgctctcccg caggtaaagc
    18361 ccgagagagt ctgtaatgtc acccgtttcc ggggcgtttt ctgcatctgc taacgcttct
    18421 tcgctgatct cttccacggc ccgaagctcg tctgcgctcg gcgctgcgag accatcggtt
    18481 cccgtttctg ttatttgctc ctccatattg cgtcctccga tcgttttttt cagggccctc
    18541 tatttttatt atatcatttt gcgtatggtg ttatgtaaac cgacgagcat ttacgacaaa
    18601 atttacagtt tcttaacttt ttcagatcgc cccgattttg aaaagcaggg gaaaaaacag
    18661 gggggaaagt gaggattttc ttttcgcttc cccttgacat ttctgtttcc ggcagctata
    18721 atatccctgc ttaaaagaaa gcagatcgtt tccggtctca cccggccgcc cagggcgcgc
    18781 cgcggtcaac atttttcggc tctattttgt atggggtcgg agtgtgtgcg gatgcggttt
    18841 gcgtatccgc ttttttgcat ccatgaaaag gcgcgagagc gccggcgact tatttggagg
    18901 tgtttaacga tcgctaccca gaatcatttg ctcaacgaag agatccgcga accggaagtt
    18961 cgcctgatca gcgaggaagg cgaacagctt ggcgttatgt ccggcgagca ggcgctccgc
    19021 atcgctgaag agcgggagat ggatcttgtc ctgatctctc cgcaggcaaa gccgcccgtg
    19081 tgcaagatca tggagatccg catgtccccg ggcattggcg agaacgattt caataccaag
    19141 ctgaagaacg gacagaaatt cctcctggac ggcgaccggc tgaaggtcac ggtgcgtttc
    19201 cgcggccgtg aaatggcgca caccaacatt ggcgagcagc ttctcacgaa ttttgccgct
    19261 cagtgtgccg agatcgccac catggacaaa aaccccaaga tggagggacg gaatatgttc
    19321 atgttccttt cccccaaggc ccccgacaaa caagcaaaca aaaagtaaag gagatattcc
    19381 gagatgccga aactgaaatc gcacagcggc gcgaagaaga gattcaatct caccaagacc
    19441 ggcaaggtca agcgcgccca cgcttttaag agccacattc tcaacaagaa gagcaccaag
    19501 cgcaagagag gcctccgtca gggcgcctac gcggacgcca ccaccgcgcc cacgatcaag
    19561 cagatgattc cgtacaaata aggaggactg aaagatggcc agagtcaaag gcgcgatgat
    19621 gacgcgcaag agaagaaata aaaagctggg tgccaagggt tattgaggaa acaagtcccg
    19681 tcactataag atggctaacc agcagctgat gaagtccggc cgttatgctt acgtcggccg
    19741 caagctcaag aagcgcgact tccgccagct gtggatcacc cgtatcagcg ccggctgcaa
    19801 gatgaacggc atgaactact ccaccttcat gcacggcctg aagaaggccg gtatcgatat
    19861 gaaccgcaag atgctctccg agctcgcgat ccacgacgcc gccgctttca ccgccctgtg
    19921 cgagaaggcc aaggcgagcc tttaatttca cacaacgata aaaacacgct gcttttcaac
    19981 gaagcggcgt gttttttcgc tgttgctatt tcccggctcc tgtgggataa tagagcaaat
    20041 gagggaaata aggaggaatc cgtccatggc attctgcacg aactgcggtg caaagctcgc
    20101 tgatgacgcg aaattctgca ccgaatgcgg tgcaaaggtt gaacccctgc cgaccgaacc
    20161 ggtcaccgaa ccggtaacga ctccggcgcc tgcgagcgac gcggctccgg cgaacgacta
    20221 tacccggcct gtgagcgact atacttacac tccgccgtcg agcggctata cctatacccc
    20281 gaccgggcaa aacaccgcct cttccggctc atactcgccg gaaataccac gcaataccta
    20341 ttcctatgac cctgccgcgg ccacccccgg cggcacgcag ccgccgcgcc cgccgcacga
    20401 tattcccggc ggcagccagc cgcagaagag aaagatgtcg ttcctgccgt ttttgctcat
    20461 cgccattggt gtcgcggcgc ttgtgttcct gctgatcggc gtcttcggaa accgcagcgg
    20521 cgatgtgtac aacgcggtct acggcaagat cggtctgcgc accgtcgcag ccgaggatat
    20581 gtgggaagac ggcttccgca ttgagctctt ctccaacgga aaatgccgcg ccacggtcgg
    20641 cgacgaaacg gagacctgcc gctatgagct cgacgatgac ggcaatatcg acgtactgct
    20701 ctatgacgcc gagatgcacg gcacgctcag cggcgatgtg atcgtctttg aaaacgtgtt
    20761 tgactccggc atggatctct acttcgtccg cgaaggcgga tcgatcccgg cggagggctc
    20821 gaagccggtg ggcgggcaga cgaccgaagc cccggctgaa gctccggttg ataccggctc
    20881 agactatgcc tggtgggatg gcgactggta cggctggtgg gtcatctatg aaggcggcgg
    20941 agattatgcc gatctggagt cttattgcta cgatgcctgc gccacgatct ccgtatatgg
    21001 caatacgggc ctcgtggatc tctgggacga ggactgcgcg gaaggcatga acatcggcta
    21061 tatcgatgtg gcctttgagc cgggtacgac ggacaagggc cgcatggtct ccgtaagcgg
    21121 ggatttctac gacgatgaga tcgccgcgag agactgggtc gttgaccccg gcgactcgat
    21181 cgtcagccgg tttgactcca tgatcctgat cgacggcatg tactatgagg acggcaacag
    21241 ctcgaactgg atccattatt ttgtgtttct tcgcccgtgg ggcatgagct gggaggacgt
    21301 acgcagcgac tcctgctacg atatgcccta tgacaacatg ctgccgctcg gttacgatag
    21361 ctggtatgtg ccgcatatga acgacgccat gccgtcgtat atcgatctcg atcgcggcga
    21421 agccgcctga cagaataaca cgcaaagacg gagcagggaa gacctgctcc gtctctgcct
    21481 gtcgaaaaag cctcgcagag tttgcgcccg caggcgcaaa tagagtgtta atcattttct
    21541 ccggcgacat gtgcgtcgga gaaaattcat ctcggctcgc aaacgtgcga gcgtaagcga
    21601 gtgcgctgcg gcgagccgca gccttttcaa acgagagccc aacgaagtgg gtctcgtttg
    21661 agagcttgtc aaaaatactt ttttgacaag ctcagacgga gcagggaaga cctgctccgt
    21721 ctttttttta ccggttgtca tccggcgccg gacggtgtaa aatgccggat atggaaacaa
    21781 cggagaaaaa gagaataacg ctccggccgc tctggaggct gtgtccggcg cgccatgcgg
    21841 tgctgctcat ttcgctcgcg tggctcgcgg catactttct gctgcgcgaa aacagggcgg
    21901 tgatgaactt tctttgcaaa gcgctcgtgc gcccgtggca cgcctttgcg ggaaggctct
    21961 tttccgccgt gccgttttcc gtgacggagt gggtcatcct ctttctggcg gcgctcggcg
    22021 tcgtgctgct cgtccttctg atcgtgcggc tcatccggcg gcggtgggtg aaggcttacc
    22081 gcacggggat gacgatcctt tccgtctctg cggcgatgtt tgcgctcttc tgtctctggt
    22141 ggggcgtgct gtattattcc gacagcttca ccgaacaggc ggggctggaa cggcgcgata
    22201 tctccgtgca ggagctggag accgttaccc gatatttcgc tgaacaggcg agctcggcgg
    22261 gtgagcatgt agagcgtggc gaagacggcg tgttccgtgc cgacaagagc gacatcttcc
    22321 gccgttcccc ggatatttac gggggcgcgg agcagatctt cccgtgcctt gctgccccgg
    22381 cggtgcgggc aaagcccgtg ctgctctcgc ggcttttgag ctatatccgc tataccggtt
    22441 tctttttccc ctatacggcg gaggcgaatc tcaacgccga ttcgcccgcg tgcctgctgc
    22501 cgtccacgat cgcgcacgag ctggcgcatc tgcgcggcgt tgcgcgcgag gacgaggcaa
    22561 acttctgcgc cgtcgtcgcg tgcatggaaa gcggcgatga ggattaccgc tattccggcg
    22621 cgctgcttgc ctatatctat ctcggaaacg ccctctacag cgccgactac gacgcctggc
    22681 gcgaggtata ttccacgctc agcgagaacg tgcgcgccga tctgcgcgcg aacaatgact
    22741 actgggcgcg gtttgaaacg cccgcggcgg acgtgtccga aaaggtgtat gaatcgtttc
    22801 tgcaaaccta cggcgacgac cgcggtatgc agagctacgg cgcatgcgtc gatcttctga
    22861 cggtatatta cttagacacg gaatgagggg ctcctttgtg caaagggagc tgtcagcgat
    22921 gctgactgga gggattgcaa actccgaaac tttgggaaac ttatagaaaa aggcttctcc
    22981 ccaattgagg gagaagcctt ttatagagtc gccttatcaa atcaagaaac ggagcctatc
    23041 atagatgcca tcgagcctac aagagccagc gcacttatgg caatgataac agctatgcca
    23101 gatccgatca gtccaatcag gctgcagacg aagccacctg tccgaattcc ctccgtgtta
    23161 ccagcttttt ttgcctgacc agccaatacg agaccgatta tgccgagtat aatgccaacc
    23221 agagctccaa ccccaaaaaa ccagcagacg attgcacaaa ttcctaaaac aagcgaggct
    23281 acggctttgc cgtgcatgac agaagtttcc attgcgattc tctcctctgt ttgtattgtg
    23341 tttacattat acagttgaga acggtaaaaa acaactgtgc aatatatata tatatatata
    23401 tatatatcac ttcttgtgca ggatgatgaa aatgcctctc ctacaaggat gaaatttacc
    23461 ttgtcaagag aggccaaaat gggtttattc cagcgctaag ccccagtaga tcggctccgc
    23521 gccgttgagc cccatgagaa acggctcgtc gcctgcgcgc gcggggagcg cctcgcacac
    23581 ccgtccgccg tccacggcgt aaatgtcgcc gcgctcgccg cagaaaaact ccccggtaac
    23641 ggagaagagc ttgagaatcc cgtcgctgta ctttgcgtgg gggataccgt caagccacac
    23701 ctcgcggcgt gcgcggaatt cctccgctgt gatcttccgg cgaacagccg gaagcggcac
    23761 gcttgagcgg taggataccg ttctggcgcg catcgtgagc gcgtaccagc accgaagcgt
    23821 cgcgctcgcc gggcagagat acgccggctc gcctcccgca gcggcgaaca ccaccgcccg
    23881 gccatagccc cggccgcgat actccggctt tgtcgctacg gcgaagatgt accggacggc
    23941 gccgaacggc acggcgtacg ccgcggcgac gacggaaccg tcttcctcga tcacgctcgc
    24001 catgccgggc tggtagacat tctgaaagaa cgcgtcgata tattcgtccg tatcgccgaa
    24061 cacctcgcgc cagagcgcct tgagcgcatt ctcgtcgccc ttgcgggacg gacgcggcgt
    24121 catgcttcgt ccccctgcca gagagcggtg aatttatcga tcatgaacat cggataccat
    24181 tcctctttcg cttttcgcag accggggatg cccatatctt cctcgcggtt gagataccgg
    24241 atctccggaa aatccttttg cagatggcgg gcaaactccc gcgcgatcat cggatacgcg
    24301 ccgggaagat cgtcgggcgc tttttcaaaa tggacgtcga tcgtgtcgct gccgagccgt
    24361 tccccggcgg tgaacgccgc cggtctgccg tcggcataga gaatgccgcc ggtcatgccg
    24421 agcccgtccc agtactgaaa catccgctcg atcgcctcgt tttcctctgc attgccgccg
    24481 ttcttttcgg cgtcccagct ttggagaaga ctgcggcagt cgtcaaatcc ggcgggagag
    24541 agcgcttcat agcgccagtc gtgcgctgtc tcaaagcggt tgcagaaatt gcgcttgccg
    24601 tggagcttct ttccggagag cgtggcgaac gattcaacgg aatagatata gtccgcgtcg
    24661 tcccgggcct caaaaaaccg gaaccgtccc ggcagagcag cctcgagcgc ggcgcgctgc
    24721 ggcgcggtca cgccttccag atagagcggc cgcccctgct ccgcggcacg cgcgcggagc
    24781 gcgtcaatgg cggggacgag atcgccccgg ccggaggggt aggcgtagaa agcgcccttg
    24841 gagcaaaggt attcaatgcc cagccgctcg ccgagaacgg caacattgcg gcggcagaga
    24901 atgtcccaga gaaatacgtt tccgaagctg tccgaggagc ttttggagtt ttcccgggaa
    24961 agcgcttcgt caaacaaagg cttgtgctgc agcccaatgg gctgaaattc gctcataaaa
    25021 atgacctttc cgtttgttag tttgttattt atagagccgc tcgcagacgc gctttgcctc
    25081 gctgcgcagg gatcgcaggt cggcgaacgt ctccgggcgt ttggtgatgt cgcgcagcag
    25141 cgcggacgga tggtagatcg ctatggcgcg ggcgctttca atgtcgaacc actgcccatg
    25201 ctcgcgggtg atgcgaaagt ccggcttgat gaccgccatc gcggcaatgc gcccgagaca
    25261 gacaatgagc ttcggacgga tcagatcgcg ctgcgcgtcc agccagtgac ggcacgccgc
    25321 ctgctcttcg ttgagcgggt cgcggttgtg cggcgggcgg catttgacga tgttcgtaat
    25381 atatacgcgc gaacggtcga gatcgatcat ttcaagcata tcgtcgagca gcttaccggc
    25441 ggggccgacg aacggctcgc cctgcagatc ctcctgctcg cccggtcctt cgccgatgaa
    25501 catcagctcg ctctcgggat cgccgacgcc gaaaacgacg ttgtgccgcc ccagtgaaag
    25561 agggcagtcc cggcaggaga gacattctct ttgcagtttg tccatcgctt ccatgatcat
    25621 cacactccat atagaagcat gttatcacgc ttcgatagaa aaggcaacgc cggattcgct
    25681 ctcacggagc tggcggtacg tttttttgat cagaacgacg ggaatgacgg cggtgaggat
//
LOCUS       DAWAAE010000015        24635 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_876_length_24635_cov_3.646908, whole genome shotgun sequence.
ACCESSION   DAWAAE010000015 DAWAAE010000000
VERSION     DAWAAE010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 24635)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24635)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24635
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_24635_cov_3.646908"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16389..16398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18472..18481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20071..20080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21124..21133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5427 a         7107 c         6452 g         5609 t
ORIGIN      
        1 aggactgcgg catcaaccgc atgacgtttt actaccactt caaggacatt tacgatctcg
       61 tggagtggtc atgtctggaa gacgcgcgcc gcgctctcga ggagaagaaa acgcacgaca
      121 cctggcagca gggcctcctg cagatcttcg aggccgtgcg ggagaacaag ccgttcattc
      181 tgaacgtcta ccactgtgtg gaccgcgagc aggtcgagcg gtatctccgc ccgcccgtgg
      241 accatctcat tctcggcgtg atcgacgagc agtccaccgg aatgaccgtc cgcgatgagg
      301 acaaggcgtt catcgcgcag gtctacggct acattttcat cggcatcatg ctcgactgga
      361 tccgcgacgg catggccgag gagccggaga agatcgtgga ccggctcgcg cttctcatcc
      421 acgattcgat ccgcgaagcg ctcgcgcgct ttaaaattta aacaaaatct ccgatttgat
      481 caaaaacttt acacttcgcg cccccgtgat aaaaccctta tcacgggggc acttctgttt
      541 atggtaaata aaattctttg gggtaatata aggccatcaa aagaaaaaca aatctcacag
      601 gaggtcaata ttatgtatta ctctgccgga acgtatgaat ccttcgccca ccccgaaaag
      661 ccggagggcg tcgacagcaa atccgcctat atcatcggca ccggtctcgc cggtctgacc
      721 gcggccttct atctcgtccg cgacggccag atgaagggcg agcacatcca tcttctcgaa
      781 aagcacgagc ttgccggcgg cagctgcgac ggctaccgcg acatcacgaa gggcttctat
      841 atgcgcggcg gccgcgagat ggacaaccat tttgaggtca tgtgggacgt gttccgcgat
      901 gtgccgagca tcgagacgcc gaacgtaacg gtgcttgacg aatactactg gctcaacaag
      961 cacgacccca actactcgct ctgccgcgcg agcgtcaacc gcggcgagga cgcccacacc
     1021 gataagaaat tcgctctcga caaagcttcc gccatggcgc tctccaagct ctttctgacc
     1081 ccggagaagg atcttgagga caagaagatc tccgaggtgc tgcccgagag cttctggagc
     1141 acgaacttct ggctgtactg gcagacgatg ttcgccttcc agcgctggtc gagcgcgctg
     1201 gagatgaagc gctatctctg ccgctacgtc caccacatcg acggtctgcc tgacttcagc
     1261 gcgctgcgct tcacgaagta caaccagtac gagagcatga tcctcccgct cgtgaagtat
     1321 ctcgaatccc acggcgtgcg catcgagtac ggcatggacg tgaagaacgt cgtcatcgaa
     1381 aagcacggcg ataagagagt cgccaaggcc atcgtttacg tgaaggacgg ccaggagcag
     1441 acgctcgatc tcattgagga cgatctcgtg ttcatcacga acggctgctg caccgatacc
     1501 tcctgctacg gcgaccagac ccatgcgccc gacctttccg gcatcgagaa cggcaagggc
     1561 gagagctggg atatgtggaa gaacatcgct gcgcaggccg agaacggcga atacggcaac
     1621 ccggacaagt tctgctccga cgtgaacgcc accaactgga tgagcgcgac cgtcgccacg
     1681 gcgaacgagg agatcatccg gcacatcatc aacgtctgcc accgcgaccc gcgcgagggc
     1741 aaggtcacga ccggcggcat cgtcacggtc aaggacagca ccgagaactg gtacctttcc
     1801 tggacgatca accgtcagcc gcagttcaag tcgcagaata aggacatggt gctcgtgtgg
     1861 ctgtacgcgc tcaacaccga ccgtccgggc aactacgtcc gcaagcccat gcgcgagtgc
     1921 accggcgaag agatctgccg cgagtggctc tatcacatcg gcgtgcccat cgaccggatc
     1981 gacacgctcg cgagcgtagc gtgcaacaca acgacgtgct tcatgccgta tatcaacgcc
     2041 ttcttccagc cgagaaagga atccgaccgg ccgcgcgtcg tgccggaggg cgcggtgaac
     2101 tttgccttca tcggccagtt tgccgaaacg ccgcgcgaca ccatcttcac caccgagtat
     2161 tcgatgcgca ccggcatgga gtcggtgtac acgctgctga acgtagaccg cggcgtgccc
     2221 gaggtctggg gcagcaagta cgacgtgcgc gagttgctgc gcgcatgcta ctacgccatc
     2281 gataaaaagc ctctctccga ggccgagctg aacttcggcg agcgtgagct tatgaagatc
     2341 gtgctcaaaa agatccgcgg cacggacgtg gagctgctgc tcaaagagag cggcctcatc
     2401 gaataattcc gaaaaaatcc gcaaaacagc tgccgccggt cagaaaatct gaccggcggc
     2461 tttttgcctt gacacaggcg tttgccgtgt gttatataga gaatatatct gattttaaaa
     2521 ggagggacta ccatggacag cggcagtaac ggacgaatat ggggcatggc gagacgcatc
     2581 ccagggactt cgtatggccg cgcaggccgg tgaagtctct cttttccgct gctcaccatt
     2641 gcccggatcg acgtgcgccg cacgccgggc agcggtgata tttttttgcc ctgctccggc
     2701 gcggttatca aaaaatcgtt gaaacggagg gcagaaacat ggaaaacaaa accgaactca
     2761 tgcagctccg ccacccggat tacgaccggg agctgacgga gctcgtccgc agcaatctct
     2821 cgccgaaggc gttccgcgag aagctgctcg actatcacga aaacgacatt gccgccgcgc
     2881 tcgaggcgct cacgctctct gggcggcagc ggatctatca cgcgcttgac gccgcggagc
     2941 tctcggccgt gctcgaatac gccgagccga tgacggcgta tctcaatgaa ttgagcctcc
     3001 ggcggcgggt gaacgtgctc tcgcgcatgg acgccgacgc tgcggtggac tatctcaaaa
     3061 cactcgaaag cggcgagaaa aacacgctcg tcgagctcat ggacgagccg aagcgccgcg
     3121 agatcgcgcg gctctgggtc tacggcgaag acgagatcgg cagcattatg acgacgaact
     3181 gcatcgtcat ccccgagggg ctgagcgtca aggccgcaat gcaccagctc gtcgagcagg
     3241 ccgccgagca cgacaacatc tccacgatct atgttgtgga ttccgacaac gctttctacg
     3301 gcgcgatcga tctcaaagac ctcatcatcg cgcgcgaggg cgatccgctc gcagagctga
     3361 tcgcgacctc ctacccctac gtttacgcgc acgagcgcat tgaagacgcg ctgccgcgca
     3421 tccaggacta ttccgaggac tccatccccg tgctggacga ggaaaaccgg ctgctcggcg
     3481 tcgtaacggc gcaaagcctc atccagacag tggacgacgc gctgagcgag gactacgccc
     3541 ggctcgccgg tctctccgcg gaggaggatc tcaacgaaac gctcgtgcag agcgtgcgca
     3601 agcgtctgcc gtggctgctc gtgctgctgg ggctcgggat ggtcgtttcg agcgtcgtcg
     3661 gcctttttga gagcgtcgtg gcgcagctca cgctcatcgt atgcttccag tcgctcgtgc
     3721 tggatatggc gggcaacgtc ggcacgcagt cgcttgccgt cacgatccgc gtccttatgg
     3781 acagcgaaac gaccggcgca cagaagcgga agcttgtctg gaaggaggcg cgcgtcggac
     3841 ttatgaacgg cgcgatcctc ggcgcggcct cgttcgcgct catcggggca tatcttctgc
     3901 tgcgcggcga agcggcgctc atggcgttcg gcgtttcggg ctgcatcggc atcgcgctca
     3961 tcgcggcgat gctgctctcc agcatttccg gcacggtgat cccgatcttc tttaagaaga
     4021 tcggtgtcga tcccgccgtt gcctccggcc cgctcatcac gaccgtcaac gacctcgtcg
     4081 cagtcgtatc gtactacggc ctcacctggc tgctgctcat cgaatggctc catctggcat
     4141 aaaaaacgct cccccacctc ggatgtcttt tcccgaggcg ggagagcttt tttgctttgg
     4201 ccggttggtt tgattatact gcgcggtaga tcggctcccc atgcaggaag gtctccacaa
     4261 cattctgcgc cgtttttttc tgcaattcgg caagcgccgc ttccgagcac cagcttatat
     4321 gcggcgtcag aatgacattc tcgcgatgca aaagcgggga ctcgctgaaa ttttcggtgc
     4381 caagcacgtc cagcgcacag taggaaatgc ggccactgtc gagcgctcgc atgagcgccg
     4441 cttcgtccac aagtccccct cttgccgtat tgacaaatat cacacccggc ttcatgcttt
     4501 ggaattcctg ttcaccgatg atgcctctcg tcgccggagt tagctgcaaa tgcatcgtga
     4561 caacatcggc gcgggaaagc gcttcatgca gttccagaaa ttcaacgtcg ggatacatcg
     4621 accggtcagt gatatagggg tctacggcga ccacatgccc gaaaaacgcc cgcattttac
     4681 ggtacagatt ccgggcaatg tttccaaaac caacaaagca gatcgtgctg ttttccagcc
     4741 gcagcattgg gcgcaatccg ttcatctcga aattcccgtc cattagacgg tcacgcgaaa
     4801 cggatatgcg ccgtgctgca ttaagaatat gcgccgccgt ggaatctgcc acctcgtcca
     4861 agcagtaatc cgtacagttt gccaccataa tgccgcgctt tttcgccgcg gcgagatcca
     4921 ccgtgtccag tccgatcgca taccgggcaa tcacccggca tttttccagc ccatccataa
     4981 acgccgcatc gatcggagcg gcaaactgcg tgatcagcgc atcgcagtcg tgcgcatacg
     5041 taagaagctc cgtgggatcc ttgatcccgc ccgggcggat ctttgtgcag tcaacaactt
     5101 ccacgtcatc acccaaacgc cggaattctt ccagctcttc atgtatgtcc ggatagtagt
     5161 aatccgaaat aacaatctta tattttgcca ttggctcagc ctcgactttc ctgctacatc
     5221 aatgatttcg gaatcagcgt aatcatcccg gggaacgcca cgcacagcag gacggtgatc
     5281 accagtattg ctatgaatgg ccagattttc ctgcatatgt cagtaaagga gcattcggcg
     5341 ataccagatg tgacgtacag cagtacgccg accggcggcg tgatctgccc gatcaggagg
     5401 gaaatcacca tgacgaccgc aaagaatatc gggttgaatc cataggaggt gcacaccgcc
     5461 gaaagaaccg gtgcaacaag gatcatcgcc gccagaacct ccacaaaaca ggtaagcact
     5521 agcaggaaga ggataaggag gagaagcacg atatacgggt tgctcgtcag ctgtgcgagc
     5581 cacgcaacgg tgtgctgcgg gaacatcgcc acgcccataa gccacgcaaa cggaccggcc
     5641 attgccgcga taagcagcac acttgccgag gtcagtgccg acttgatcag gatcttgggg
     5701 atgtcacgga ttgttagcgt ccggtacaca aagagaccga tgatcatggc atagagagaa
     5761 gcaacgatgc ccgcctccgt agccgtaaac aagccggaga tgataccgcc aatgatgatg
     5821 accggcgtaa gaagagcaag gaccgcattc aaaaaggcct ttccgagagc cttccatccg
     5881 atgaactttc cgtccggtgt taggtacgga aacttgtcct ttcttttcgt cgccagaaag
     5941 gcaacataca acatgcacaa aacgccgatg cacacaccgg gaatgatgcc gcccataaac
     6001 atcgcgccaa cggaaatgcc tgtcagcgaa ccatacacga tcatgttcat gctcggcgga
     6061 atgatgggtc ccatggtaga gaccgccgct tgcagcgccg ccgtccagcc cttatcgtag
     6121 cctttttcct tcatggcggg gatcattacg ccgccgatcg ccgcagcatc ggcgaccgag
     6181 gagccggata ctccggcgaa gatcatgccg gagaggattg agacctgcgc cacgttgcca
     6241 ggcagatggc cgaccgccgc cgtagcaaac ttaatgaggc gcttggatat accgcccgcc
     6301 gacatcagat caccggcgag aatgaaaaac gggatagcaa gaagcgaata gctgttcagc
     6361 atgctgaacg tccgctgcac cacgacctcc agcgggaacg tgctgccgct gagtatcagc
     6421 gcaagcagcg aggaaaagcc catgctgtat gcgatcggag cgccgatcag gagaagaatg
     6481 acaaaagaaa caaaaagaat gatcagcatg gctcgtcacc tcccaacgga tgtttcagga
     6541 tgtatttttc ataaatgtcc acaatggtat aaaacagcat aaggatcggc ccgatcagca
     6601 tggcgtaata tacgtagccc atcgggatcc ccagcgtggg gctgatcgtg gcgatgttca
     6661 cacgaatcat tcggggagca aggacgataa acagaatgaa caatgccagt gaaacgagct
     6721 cgatcaaaac ctccatcacc gcctttacgc gggggccaac attgccgaga acaacgtctg
     6781 cggcaacatg cttctttttg cggatgccat acgccgcagc gatcatgacc atccatatga
     6841 agagatactt ctgcgcctca tctgcccaga caatgggcag ggaaaaagca tatctggata
     6901 tgatctgcat cagtccgata ataatgatgg cgctgaaaaa aagaacgatc agaattctta
     6961 cgatcttgtc tacgatggac acaaaagttt tcacgtttta acccactcct ctccgggaga
     7021 gagcctccct gcgcagggca ggaaggctcc cctggtaata ctgcagccct cactcagccg
     7081 acgatcttgt tataaacgtc cgtccagccc ttttcctcgc agtactttat attaaactca
     7141 ttgacggctt tggcaaagct gtccacatcg acttccacga tatcgacgcc ggagctttcc
     7201 atttcggcaa gcgaaactgc ttccttctcc ggagccatcg ctgtgtgggc tgcagaggct
     7261 tccgcagccg cagccagaag attcttctgc tgttcttctg tcagcttctg gaaggagatc
     7321 tccgacataa agagacccat ggggccgcac atatgctttg tgagcatgta atgatcgatt
     7381 acttcgtcaa acgcattggc aagagaggta ttgcagtcgt gctcatagcc gtcgataaca
     7441 tgggtctgca gagaggtgta aacctcgccg aagcccatcg gcgtcgggtt caaccccata
     7501 gcctgcatgg tattgatgta aacgtccgat tcgatggtgc ggatcttcag attcagcgct
     7561 tttgcctcgt cgagattggt gaaagcattc ttctcgccga ccgtgcccac gcagcgatag
     7621 ccaaaggaga cgctataggc aagaatgcgc acgccggcag catccagaag gcccttggca
     7681 agctctgtgc cgacttcgcc gttcagaacg ccggccatct ggtcgtagtc cttccagata
     7741 tatgccagat ctagtacgcc aagcggcgca tacagcttgc cgtaatccgc cgtgccggta
     7801 atgttgatat cgatggtgcc catctgaacg ccttccacca gcgccgcact gtcgccgagc
     7861 tgcgcaccgg ggaatatctc gattttgatg ccgtatttct tgcaggcttc cttcaaggca
     7921 agcgcatacg cctgcgcaac gttgtcgccg tcggactgca catgcgcaac gttcagcgta
     7981 acttcatatt cgggttccgc ttctgtctcc gcgggttcgg cggtttggga ggtcttggtt
     8041 tcttccggct tggtttcaaa ggcggagctg cttccgcatc cggcaaagag cgtaatgatc
     8101 aaggcggcgg cgcagaggag cgcgagaaac ctgatttttt tcatggtctt tttcctttct
     8161 tttgtctttt tctcttttac cggcgattac tcgccggata tttcttcacg aagttgcttt
     8221 gccgcggctt caagatcctt ttcctcgggg tacttcgcat tggcgttcat ctggaaaata
     8281 ccgttccaat ccaacccatc ggcatatttg gggacaatat ggatatggaa atggcgcacg
     8341 gtatctccaa acacaccgag gttgatcttt cccggttgat acagcttttt cagcgctgcc
     8401 gtgacgcgcg caagatcctc ctgcagcgct gcgcgttctt ctttcgtcag ctcgcagaat
     8461 tcctccgcat ggcggcgaag cacaagtatg cagcgtccgg gatagctctg ctcgcggttc
     8521 agataaagat ccgatgtgga gagctcgcat acgggataag tgaagctctg cattttttcg
     8581 ttcttttcgc agtagagaca gcccacggtg tttcctcctt tactccactt cggtcatggg
     8641 gatgacatcc atatcgggga agtccttgtt ctcgccgccc atcgcccaga taaaatcgta
     8701 atgcgttgtt ccgacgccga aatgcatctg ccacgccgca gaaacgatcg cctgctcatt
     8761 gcggaggatt acatggcggc tttccttcgc ctcgccgatg acttcgatat aaaacgcatc
     8821 ctccggcata ccgtaataca tgaagcactc cacgcggcgc ttgtgcgaat gcgtcgggat
     8881 ggtgttccat acattgcccg gctgaaccga attgatgccc atctggagct ggtcgctttc
     8941 cacagggccg cccttgtaaa tgtacttctt cacaatgcgc gtgctcgccg tcgtctgatt
     9001 gccgcagggg ttgaactggg ctttcttcgc cgggatatga actgtgggat gcttttcaaa
     9061 ggaaagaagg ctcgtcacat agaactttgc cggtttttcc gggtcttcgc tggaaaagac
     9121 cacgtccttt gtgcggcagc caatgtatgc gccgtcctca aagtccattt ccataacctg
     9181 accatcggca acgatctttc cttttccgcc aatgttgatg acgcccattt cgcgtgtctg
     9241 aaggaagtat tcctgccgga tctcatcctg cggctccaga cggatctcgt tgcggacagg
     9301 catgataccg ccgagaataa atcgttcgtc aaagctgtac gtcagcacgg cctcgtcggg
     9361 agcaaacacc gtcttaatca ggatccggtc gcggatctgc ttcgttgtca gggttttcag
     9421 ttcaaacggg gaaatactgt acttgatctc catggttttg tcctcctgct tctctttatt
     9481 tttctgaact catttcagaa ttatggtttt tcacataagt cccagctcaa ccagtctccc
     9541 gcggatatgc ttcttttcct cctcggaggc cgggataaac ggcgatctcg ggtaggtctt
     9601 caaaatgccg cggatctcca gcatagcata gatcgccagc tgcgtagaac gcgcgagata
     9661 catgatgtct cgcatttcat tgacgcggaa ttgtgtctcg cggcaggagg tgaaatcgcc
     9721 ctccatgcct tggcggaaca tggtttcgca gatctctggg aatgcgttgc cgatgccggg
     9781 gataaaagca cggcagccca gcacgcacgc ggaaagccac atggcctctg taccaagagc
     9841 aacgtcaaat gtatcgcttt tcaacgtgcg catgtagttg gccatcgcca taatgtcgaa
     9901 agtggcatcc ttaatgccgt ttacaccgac ttcctccttc agccggcgga tcgttttcag
     9961 atccatggta tacccctgga aggaaacgtt gttgtaaaca tataccggca cacgcccatc
    10021 tacggcttct acgattttcc ggtaatactc gcagatctga tccggattgt gcttgaaata
    10081 gaacggccca accgatgcga ccgccgcagc accgacttct gcggcatgtt ttgccagccg
    10141 cgcagcggaa tagctgtccg ccgtgccgat gtgcgccaca acaggcactc tgcccgccgc
    10201 cgtttcgatc gtcttttccg tgacggcgcg gcgttcttcc tcggtcatca tgacaccggc
    10261 gccataagag ccattgatga acaggccgtc cacctcgccg ctgagatagt cgacaagcgt
    10321 atgcagcccc tcaaagtcca cctccccggt ctcgcggaac ggcgtaatca tgggaggaac
    10381 tacaccatac atctgaaaca tttttcttct cctttccagc ctcttatgta aagtttttgg
    10441 atgaaccctt atgcagtgcg atcgctatat cgccgttgac tgtcagccgg tcctgcgcct
    10501 ctgcgccagg gatacattcg atccgcagat ccgcgccgaa ctcctgccga agcatgtatt
    10561 cgtacatctg gcacctctcg gcgtggccgt agaaaatata ccgtccgccg accatccccc
    10621 aatttcgcat ctcccgaaag gcgtacatat catccgccgc cagagcggca tcggtaaaga
    10681 tacgccgctc atcgctgttc gtgcgcatca gcacctgcat aaagcgcggc atcagaagcg
    10741 tgcggctcag accggcgttc tttacacaat ctgcagcaat actgaccaac gcctcccacg
    10801 agtacccgcc ggagggctca gcttcgcatg gctggaggct tttccctata ctcgttgccg
    10861 agcagatggc ctcatacagc tgaccgctca gcgttgtgtt cgatgccgcg atgcggttgt
    10921 tctcatccac atagattaac ttcgtatgcg agctgagagc cacaatcgtg cacggccccc
    10981 gattctccgt ttccgtaaga atgcccatga cctgcgcttc ttcgccgcgc ataaagtcta
    11041 cagtgcggag ctttcccgcg cgtacatctg ctccatagtc gttgcggatc ccgcggatga
    11101 agtatacagg gcaggcaatc ggcagcacct cgccggattt tgcctcataa atggactcgg
    11161 ccaattcgtg aagccccgca ggcgcaataa gatgcgggat ctccatcaac ccaagctcag
    11221 aggtgatcat accggaggcg atcatatagg aaatatccgt ttcttccagc cccagcgtgg
    11281 aaagcgtttc gcggaaaagc ttcgttatcc cgctgcgtag cgtatcgcga ctgccggtga
    11341 tcgccgtatc tcttactccc accttttccc ggccggaagc aaggattgaa cggttttcat
    11401 ctacaatata gacgcgcgtt gtggtcgttc cacaatccag tactgcgaac attttcaccc
    11461 tccatagttt tattagataa aacttggttt taatttattc tgtcaaaaac cgaggtgtct
    11521 atgccacctc agattttgat ttgggcttat ctcttcatat agcccagctg ttcagagatc
    11581 tttgccgccg tctgccgaac catccgttca tattccgaac ggtttgctcc gtttatctgc
    11641 tcctttatgc cgcagaggct gatccccgcc accatgcgtc cgtttacatc atagattgga
    11701 gcgcagagac tagcgatata caggtcactc tctccctggt tgacgtcaaa ttcttccgct
    11761 ctcgcgcaac ggaccatcgc aaggatcttc tgcttatccg ttaccgtctc cggggtgatc
    11821 tgcttcggag cggtccgatc cagatacgcc gcgacctcct cgtcggtatc ctgcgcaagg
    11881 aagagccttc cgataccggt agcatacagg ttcgtttccc cgccggttcg aaccataagc
    11941 tgtgcccaca caggaacgct gcttggcacg acctgctcga ccatgatcag catatcctcc
    12001 tgccgcacac cgagatagca gatcatatcg gtttcgcggc tgagcgggcg cagataccgc
    12061 agcgcgatcc gccggaaatc cagcgtgttc atctttgcca tgccaagccg gaacacgcac
    12121 acgccgagcg agtagcggcg ggtattctcg tcaaaggtca gaaagccgtt ttcgcagaag
    12181 gtgtagacaa tgcgcagcat cgtgcttttt ccgatcccgg aaagctggct cagctccgtg
    12241 agggtatagc tgctcttctc accgtcaaac agcatcagca ctttcaggcc gcgttccaat
    12301 gcggtaattg tataatctcg gggctctttt ttcattgttc cgattcctta ttgcctgtat
    12361 agatcacatg atccgtttct tgctttctat actatggttt tgtgggcgcc ccgtcaatcc
    12421 tttattttgt caggtatccg ccgtccaccg gaatgattgc accgttgata tagtcggacg
    12481 cgctgctggc cagcagaacc gcgacgccct taaaaacgtc cggctcgccc cagtccccca
    12541 tcggaatgcg ggctttgacc gattcatacc tcgccgtatc tgccaccagt gccgcagaaa
    12601 gacccgtttt gatccagccg ggagctagcg cattcacgtt gatccccagg gcgccccact
    12661 cactcgcaca cactctggtc agacccgcaa cagcggactt gctcgccgta tatgcagccg
    12721 tattgaatcc gccgaggaat gacatcatgg aagccagatt gatgatcttg ccgcggctct
    12781 tctgctttac aaactgccgc gccgcaagct gcgaaaggaa aaacactgat ttctgattga
    12841 gctggatgat ctgatcccac atctcctccg tatattccag cgcggaacag cgcggacata
    12901 tcccagcgtt attgaccagt atatccaacc ggccatagtg ctccagtgtt ttttccatga
    12961 tcaccggaag taccttcatg tcgctgacat cggcatggat cgctagaaac tcgccgccga
    13021 tgctttcaat atattgccgc atctccgtcg gctcatgatg accgtagaca caggcgattg
    13081 aggctcccgc ctcggccaat ccccgggtga tccctccgcc gatgcccgac ccgccgcctg
    13141 tgatcagtgc aactttccct ttcaggctca aatcgtccag aatcataccg cttgcctcct
    13201 gttgtttcat tctttaaaac tctgttttat atttatatga tatcacactc taattgttat
    13261 tgtaaatgcg taaaactggc cgaatattcc tccagcagat tgttaaccta tccaatttgc
    13321 ctttaaaaaa taaaactgtc acgcaatcga gccaaatgcc cgtctcatgg tttctgtatt
    13381 tgcaaaaacc gggtgcagcc atttcaaaaa gctgcacccg gttttatctt atttatgccg
    13441 caaggaatcc gcactttgcc acgctataga gcaggacgaa cggcagaagg acggggcgct
    13501 cttcacatac gcctgatacc ccggatctgc gccgtagctt ttgttctggc gcagatccag
    13561 ccgccgcacg ccggaaaaca tcacgaacac gatgccgatg tatccgagca gggcgggcag
    13621 ccactgcccg ccacgcagga tgttcgcggc agaacgatct gccgcgactg gtcattttga
    13681 tacgccctgc acctctgcag agcctttcag cgggactccg caggtatccc tccgcgtcct
    13741 cctcgtcctc gagcaagagc gggagtgtgg cttcattctt ggtttcgggg gcgctctttt
    13801 cctccgcctg tgcggtgtaa ttgcgcacga tggctgccgc cttctcgatc ggcaggagcg
    13861 taccggcgcc cgcgacgcag ccgcccggac acgccatgcc ctcgagcaga tacccgtcgt
    13921 atttgccggc cttggcaagc gtgagcattt tccggcagtc cttgagcccc tgcgcacacg
    13981 cggttttcac ctcgcggtcg gggtcgatgt gcgagatcgc ctcgcgcacc gcttccgcaa
    14041 cgccgccgga aacggcaaag ccgcggcccg ccgccgtgcc ttcgcgcaac ggcgcggtct
    14101 gggtgatctc attaaagttg atgcccttgg cctcgaacat gcccatgacc tcttcaaacg
    14161 tcaggacaaa atcgacttcg ctgcgcacgg tcgtgcgcat ggcttcaagc ttctttgcgc
    14221 tgcacggccc gatgaacgcg accttctcgc ccgggtgctg ctgcttgcag aggcgcgccg
    14281 tgagcaccat gggcgtgagc gccatggaga tgcagtttgc ctgctccggg aagagctttt
    14341 tcgccatgac cgcccagctc gggcagcagg acgttgccat gaacggctgc tcggcgggga
    14401 ccttctccag aaagtccttc gcctcgtcga gcgtgcagag gtctgcgccg acggccacct
    14461 ccgccacatg ccggaagccg agcatcttca tcgcggcctt gagcttatcc ggcgtcatgc
    14521 tcgggccgag ctgcccgacg aacgcgggcg cgacgatcgc aatgaccttt tcaccgccgc
    14581 gcagcgcctg cgtgagctgg aagagctgac tcttatcgga gatcgcgccg aacggacagt
    14641 tcacaagaca catgccgcac gagacgcact tatcataatc gatatcggcg cggccgtgct
    14701 cgtcagaccg gatggcgtcc ataccgcacg ccttggcgca cgggcgctcc tgcaaaatga
    14761 tcgcattgta ggggcagaca tccgcgcact tgccgcagcg gatgcagaga tcctcctgaa
    14821 tgcggctcac gccgcggtgg ctgagcttga ccgccttttt cggacaaatc tcgcggcacg
    14881 ggtgcgccag gcagttctga cagaggttcg taacgatata gcgcttttcc ggacaggcgt
    14941 tgcaggcaaa tttcagaata ttcaccagcg gcggctcgta atatttctcc ggtctcgcgc
    15001 tctcctccgc acccgcggcg agcggcgcgt gctggtcgag cggacggggg ctcaggccca
    15061 ttgcgagacg cagccgttcg ccgacgatgg ctctttccag aaagatgctg ctgcggtgct
    15121 cggcgacctc accggggacg atcttatacg gcaggtcttc aatgcgggaa tagtccccgc
    15181 cctcgtagcc catgcgggcg acttcggtga atacctgacg gcgaatgtcg gtaatggtgg
    15241 aataaatgcc gcgaatgctc aatgaaaaac ctccttcgtt cgatttatct cgacgcgtta
    15301 cacaatatct cgctccatgc ctccggcgac gcgggggaga gatgcgccgc atctccgtcc
    15361 gctttctgcc agacgaggct ctcctcttcg acccagagcc ggtatacgct ctccgtgccg
    15421 tccggcgcgt cgagcgtgat cgtatagtcg cagtctcgct ccggcatggg tgtttcgccc
    15481 tcgtcgagca ggagataccc ggcgaaaaat ccgtcgtccg gcagcgtgac cgcgtcgtcc
    15541 gatgcgccgt tttccgcggt gacagtgacc gtaacgctgc cgtccgccgc ggcgctatcc
    15601 ggcgcggcag ccatcaacct gccgcctgcg ggcgctaccg gtgcttccgc cggggtttcc
    15661 gtttcggcac tgtcctccgc cgcgctgtac atggcgatct ccgactcttc cctcgccgtg
    15721 ctgctcgcgc ccttcgcggc gaaagtgccg gaaaaggtca ctcccgccca gatcgccacg
    15781 gcagccgccg cggcaagcgc gccgcagcgc gtcacgaggc gctggatgct cgcctttttc
    15841 ttcgccggct tttccgccgg ggcagcggtc tcgtggacgc gcgccatcac gttcgccgtg
    15901 aaattctccg gcacatcgcc gagcgcatcg tccgtgccgg atattgcctg catcgcctca
    15961 taaaagctcc ggcaggcctc gcagctttcc agatgggcac gaagctcctt gtcctcttcg
    16021 gcggtgatct cgccgtcgag cgctgcgccg atcagctctt catagtattc acagtttttc
    16081 acgcttcaca ccccttccct tgtttggacg ggaaacggtc ggaaaagttc ccgcttttga
    16141 gaagcgctat gcagagcttt ttccgcgcgc ggttgagccg cgatttgacc gtgccgacct
    16201 ccagcgagag cgccgcggcg atctcgtcgt agctcagccc cgcgctctcg cgcagggcga
    16261 gaatttcgcg gcagtcgtcc ggcagcgcgg cgagcgccgt gcgcacggcg tcccggtcgg
    16321 cggcgcgctc ggccagccgc tcgggggaat accgctcgtc gggaatggca atctcggtct
    16381 cttcgccgnn nnnnnnnntt atttttgcgc agcatatcga tgcatatgtt gttcgtcagc
    16441 tggtagagcc acacggaaaa ccggctgtcg ccgcgaaaat ccgcaagcga ggtgtacgcc
    16501 ttgagaaacg cctcctgcgc ggcgtcctcg gcgtcggcct cgtttttcag catgcgcagc
    16561 gcgagacggt atacctttgt ctggttttcc gtcacgagcg gctcataggc gctctcgtct
    16621 ccggcgagaa cgcgctcgat atatcctttt tcctcgtctt ttgtcatgct gtcacctcag
    16681 atcgcgctgt atgccgcgga cgatcttctc cgcgtcggcg agcgtattca tcgatacgat
    16741 ctgctgctta taatatcccg catgggacac cccgcgcaga taccagcaaa gctgcttgcg
    16801 cgcttccaga atggcgatct tctcgccctt gagcgagcac gccgtcgtga tctggccgag
    16861 ggcttcctcc atccgcacgc gcagcggcgg gagcggcggg acttgctccc cggcaagaag
    16921 ggcgttgccc tgcgcaaaga gccacgggtt gccgaagctg ccgcgcccga tgagcgccgc
    16981 gtccgcgccg gtgtagcgca aaatgtgttc ggcgtcctcc gcggtccata cgtcgccgtt
    17041 ggcgaatacc gggatgcgta ccgcgcgctt tacgtcgcgg atgatgtccc agtcggcctg
    17101 ccccgcatac atctgcgctc tcgtgcggcc gtgcaccgcg atcgccgcgg cgcccgcgct
    17161 ctcgcacatt tcggcaaacg gcacggcgtt gatattgccc ttatcgaagc ctttgcggaa
    17221 cttgaccgtc accggcacat ccaccgcatg gacgaccgat tcgatgatct tcgccgcgag
    17281 atcgggcgtt ttcataagcg cgctcccgtc gccggatttt acgaccttcc cgacggggca
    17341 gcccatgttg atgtcgatga tctcggggcg gcaaaggtct atcaccttgc acgcggcctc
    17401 cgccatgcac gagggatcgc tcccgaagat ctgcacggcg aggggcgtat ccccctcggg
    17461 ccggacgagc agcgttttcg tcttgccgtc ctgatacatg agcgccttgg aggagatcat
    17521 ttcgctcgtg gcgagcgctg cgccgtgttt ccggcagagg agccggaatg aggcgtccgt
    17581 cacgccggcc atcggcgcga gaaagagccg cccggcgagc tccacgtttc cgatttttac
    17641 catatggtct tatgggcgcg tcgccacaac gcggtggatg ggaagcccca tctcggtgaa
    17701 gcgcgcttcg taatcggtca tgggcgtgcc ctccggcata tcggcaagcg cccagccgca
    17761 gccgcggaac gattcgagcg accagtcgaa aagcggcccg ttgtcggtct tgaaccagac
    17821 cgccccgccc tccggcagca gcgcgcggta cttatcgagg aactccggcg cggtcagacg
    17881 gcgcttgaac tgcttcttct tcggccacgg gtcagggaaa ttgatgtaca ggcgggaaat
    17941 ctcacccggg gcgaacatct cgggcagaag ctcggcgtcc cggtcgagaa agcggacgtt
    18001 cggtatgccc tgctccacaa cgcgctccat cgcaacgacc atcgcgtccg gcaccttttc
    18061 gagcgcgagc agcagcacgt ccggctcgcg gagcgcctgt tcgcaggtga agcggccctt
    18121 gccgcagccg agctccagac ggatctcgcg gtactgcgaa aactcctccg cccagcggcc
    18181 tttatggtct tccggcgtcc ggatctgcac cgcggcgcag cgctccatcc gggggatcag
    18241 gttcggtttt ttccgcattc tcataaggtt cacactcctg tttctttcac acgccatcat
    18301 atcatagcga cgaaaaaaca tcaatccgaa aaaccttgca tggagcggtt gtgtttggta
    18361 taatatcacg gcgaaagcgc acgaccgggt atagcgaaga tggtatcgcg cctgctttgg
    18421 gagcaggaga ccgcacgttc gagccgcgct actcggacca aaaccccacc cnnnnnnnnn
    18481 ngggtggggt ttgtgcttta tttatgcgtg agaaaaattt gaatgcctct ttgttaattt
    18541 ttatgctctc taaatttgca ggcgcctttt tttgcgcggt attcgacaat ctgggaaaaa
    18601 tgtgatagta ttcttttgag gtggtgaaat catggggaca ctccagtata ttaatacaaa
    18661 tctttcaggg gttctcagcg ccgtaattgc tcttatcacc gcacttatca ccttagtcta
    18721 tgtggttttt acttacaagc aaatgaaagc gacgcaaaac gcaactgata cggcaattaa
    18781 gcaattgcaa ttaaataatc aaccatgtgt aattgcagaa atttttcgaa catatggagg
    18841 caaatgtttt tcgggaacaa gacgtcaatt gcatattgat gtggagctag aaaatgtcgg
    18901 cgattcgcct gcgcttacta cttatacctt cgcatattta gaattgcaaa acaccaaaca
    18961 tgttaagaat ggttctaata ttgtagatat ggactactta cccgatttta taaaatgtat
    19021 taaagcgaat gagaagaaaa aagcttctat acgctttgaa actttcgaga taaatatgtt
    19081 agtagaagat cttcgcatta atcacgagaa aaatgttaag cgcttaatag taaatccgta
    19141 tcgtccacac tatcgtggca ccgtgctagt cattgaagtt tattttagaa atgttcttgg
    19201 gcaatggttt aaaaatactg tacgccaaga aatagcttgg cttatcgata agcacgccga
    19261 gccaagaaaa actcacaaca taaatgagaa tacgattcct cctcgcccat tagggcaaaa
    19321 cacggaattc gaactacaac tcgtatcacc caaaatgtct gtttttcaaa cagatttaat
    19381 tgaggcaaaa gttatagagg aaaagctgtt acaatataag gatgatttga aatactgatc
    19441 cccattatct ccaaatatat acaattgaag ttgttccggt tttcaggcct tctattttag
    19501 ttacgtttag tctccccttt ggaaagcacg gtctctaagg ggagacactc ctttggggtt
    19561 attactgacc ggcagcaagg aaaaactggt cagaacttcg attttgggaa ctgtggcatt
    19621 ttatggaata taattcttaa tttgatattt gatgatttct ataataaata tttcccaaaa
    19681 ttttgttagg aggtcatcac atgttttttg ctaacaacgt ccatggcgag cgaattcata
    19741 tagacagcgc cgaacccaag ggtaagtact attgtcctgc ctgtggtgct ctgatgattc
    19801 aaaagcgagg caatataaat gcgcaccatt tttcgcataa acattgtgat aattgtgacc
    19861 cctggtatat cgggaaacta tctgcttggc acacgcgaat gcagagttgt ttcggaaaag
    19921 ccgcgcaaga agtggtcatt tggaacgatc aacacacaga ataccatata gcagatgtcg
    19981 ccctacaaat aggaaagacc aactatgtaa ttgaatttca gcactctgct atttctcaaa
    20041 acgaatttat tacccgttca tctttctaca nnnnnnnnnn gcaatgtggt tataagctga
    20101 tctgggtatt tgacttttgt gagaataaaa atcaaaaaag aattctgatt gctgatgaca
    20161 gttatgagaa gaacatcatt caactcgtat ggccaggacg tgacagaatt cgttttttag
    20221 ataatattgg tctctcgaag tgttatggcc aaatatacat cttttttcac gccaacaccg
    20281 gaaaaggaaa gaaggtcttg cacgatccgg aaggatacta tccatgggaa acgtgggaat
    20341 atatcgaccc actccataac gccccctgct ttctttctgt ccttttaggc cattttactg
    20401 ataccaccga atttttcgct aaatactatt ccgagaatga cttttacaat cgtctcagga
    20461 atttgaataa agataattag taaacgcaga attgtcccat caaataaaat gtacatctca
    20521 agccaaaatt cctctgaaat cagccgattt cagaggaatt tttcattgtc cattttctct
    20581 tcggtccatc gaatccccga ttgtagtctc aaaagctatc cggcaccaac gccagcttgt
    20641 ttatggactc atgggcacgg acaagcgcat catagacagc cagaacgtcc tcggaatccc
    20701 atgttctgag atccttttcc tcgatgacgc ctcggctctc cttgatgatt ttggcaagct
    20761 gcgcttcatc cgcaacagca acgaccgcct gaaccgccgc attattctgc gcttttttca
    20821 aattgagaat aaggctgtcg atcgagcctt tggactgcac ttcaaaaaca taaattgcct
    20881 tgcccatgtt gccgattttc gcttcccata ccgcatcaac gaccgctccg gcggtaatgc
    20941 gcacctctgc ccgactttca aagcccagga gttcgccgat cgccacaagt ttttccttga
    21001 tttcttcgtg gagagatttc gactccttga cggttgcagg cttctctgcg gcgggcgtca
    21061 ccgcagtttc gccggatggc tcttttttct ctgcaagcgg aagcagctcg tcccacatga
    21121 agtnnnnnnn nnnaagtaat ccaccgtgag caaatctgca tctttttctc ctgccgttat
    21181 cagctcccct gcaatctgct tcgcaacggc acaaacttcc gcatactttt tcccggtgta
    21241 ctgatagttg tacttcggca tatccgggac ttccaaataa gcgaagcact gtatggtagt
    21301 tttattaaag atgacatact cccgcggatt cacataggtc aacaattcgc tgatcgtagc
    21361 cggaccgagc ccctttgcat ttttcaaaaa cgtgtcccat cttttttcaa tggctccatc
    21421 cccatacagc agatccgcca actgcttttt cacatttcca aatccgttat cggcaacgat
    21481 cttgtcaaca acatacttct tgttgcccca gatcagcatg gaccacagct tgcttatata
    21541 ctcaaggaaa tcatcctctg tcatagcaag aagtttcgcc tcggtgaagg attgataata
    21601 ggactttcgc tcgctgcgct ctttccagtt atcgctgtaa tatgcagcgt ttgcggtttt
    21661 cttttttaca aactccgcaa tcgccgcgga tagtttttgc ttgttcatat aaacgcctcc
    21721 tgtgctataa ataaccgcaa aaggttgggc aggggttcgg cttttttact gcgctttcag
    21781 agcacgctcc cactcggtct ctttaaatcc gacgaggaca agatcgtccc cgaccagaag
    21841 cgggcgtttg accagcatcc cgtccgcagc aagaagccgc agcatctcct cttcgctcat
    21901 gccgggcagc ttgtccttga gttccatgga cttgtagagc agaccgctgg tattgaaaaa
    21961 tttcttcagc ggcaggccgc tgcgctcgtg ccacgctgcc agctcgtcac aggcgggatt
    22021 cgccgtcttg atgtcgcgga gctcgtaggc gattttgtgc tcgtccagcc actttcgcgc
    22081 cttctggcag gtcgtgcact tggggtaaca gataaatacc atagtctttc gctccatttc
    22141 tgtgtttggg tttttatgat agcacattcc gtaagaacgg gcaagaaaca ggataaaaaa
    22201 gcctcccgtc cgacgccggc gcatgtccac cggaaaccga acgggaggcg ttttggagga
    22261 ttatagattt gcgctcttaa aacgaagcgc tatatgctcg gcaagcaggg cgatgaattc
    22321 gccgtttgtc ggttttcctt tggctccgga gatggtgccg cggaagattt tctgctgcat
    22381 ctcgaagtcg ccgcggttcc atgcgatctc gatcgcatgg cgcattgccc gctctacgca
    22441 ggcaggcgtc gcagaaaacc ttttggcgat ctccggatac agcaccttcg ttattccggc
    22501 gacggagccc gcaccgcgga tcgtaaaaag gatccctgca cgcaggtatt gaaagccttt
    22561 aatatgcgcc ggtacgccca tctcgtggag aaggttggtg acggcgcttt ccagttgtgc
    22621 gggcgcggga gccggcacct tcggcgcggg ggccagtccg gggacggcgg cgggcggtct
    22681 cgtttccgcc ttcgcgtact gccgcaggca ctgcagaagc gctccggggt cgcagggctt
    22741 ggggataaaa tatgccgcgc cgagccttgc gcactcggca acaatggcgg cgttgacaaa
    22801 tccggtgaga acgactaccg gcggcgcgct ctccgcttcg gcgagccggc gcagtacgct
    22861 cagcccgtcg agccgcggct gaacgagctc cagcagcacc acatccggcc gcagcgactg
    22921 gatttgagaa agagtctccg tcccgccgga cgaaaagcct gccgcttcca tatctctctc
    22981 ctgagaaagc gcagccgtta tcgttcgttg gagatccgga tccgggtcgg cgatcagcac
    23041 gcgaagtttc ataatatcgc ctccatatcg attttttcct tcagctgatc tattacacgg
    23101 ataaaatagc atattttatg tcttatttca acaaatcccc cataaagatg ccgttaattc
    23161 gttcgacaat attcggcagg ggacagccgc gaaccggcat acatttgcgt ggcagggaag
    23221 tcgtagaaca cgctccactt gagctgacgg ccatctgcgg tgcgctcacc gatctgtacc
    23281 tcatcggcga agatgctctg atattgtcaa gtatcaggcg atatatactt tgattcacac
    23341 aggaacgcaa ataaacaatt ggtttgtttt tcccttgctc gtcagaattc gtcacggcgg
    23401 cgcgaaatgc gcataatctc ccgcggttgg taaacaatag gtttgccgga cattaaaaac
    23461 acaagggaga agataaatat gaaagcaacc ggaattgttc gccgcatcga cgatctcggc
    23521 cgcgtcgtca tccccaagga gatccgccgc accctgcgca tccgcgaggg cgacccgctg
    23581 gagatctata cggagaagga cggcgaggtc attttcaaaa aatattcgcc catgggcgag
    23641 ctgagcagct atgccgcaga gatctgcgaa tcgctctaca agtccacggg cgggacggtc
    23701 gccgtatgtg accgcgacag catgatcgcc gtatccggcg gcgggaaaaa ggagctgctg
    23761 gaaaaacctg tcagcaaagc gctggaggag atcatggaaa accgccgcgc cgtgcgcacg
    23821 gaatcgggca gccgcgcgcc ccggccggtc gagtcggcgg aggatcacgc catcgcggtg
    23881 gccgcgccga tcctggccga gggcgacgtg ctcggctgtg tgatgttcct ctcgcacccc
    23941 ggctctccgg cagcgacgga tctggaattc aagctcgcgc agacggtggc gggattcctc
    24001 gggcggcaga tggagggctg acggcgaaga ccgtcaaaat attttttcgt ttgggtgttg
    24061 acgggcggca caaactgtgg taatataact cttgcaatat cgcggggtag agcagttggc
    24121 agctcgtcgg gctcataacc cggaggtcat tggttcaagt ccattccccg caaccatttc
    24181 gagcggatgt aactgatttc agttacatcc gcttcttttt tatctgaaag tattgacagg
    24241 agcatcattt gtgagtatgc ttctatgatt tagcgggaaa agctgaatgg gcggcatggg
    24301 attattttta ggaggtaatg aaaatgtatc attttaagta cgtttccaaa aaagaagccg
    24361 cgccatacaa gacccagctt attgaactcc tcaacagagt acaggattat gtcagagggg
    24421 agttcacttt tcagtttaca tttatcggta gttcaagtcg taatatgatt acatatgatc
    24481 ctatggctaa tgttggtttt gatttcgatg tcaacattgc tgtgaatgac gataatgaag
    24541 attactcacc ggaggacatg agaacaattc tgcataaagc cttcttgcat tttatgagag
    24601 attacggtta taccaaatgt gagcagtcca cacgg
//
LOCUS       DAWAAE010000016        22873 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_987_length_22873_cov_3.131344, whole genome shotgun sequence.
ACCESSION   DAWAAE010000016 DAWAAE010000000
VERSION     DAWAAE010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 22873)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22873)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22873
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_22873_cov_3.131344"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5445..5454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7165..7174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4470 a         6993 c         6873 g         4517 t
ORIGIN      
        1 ccatattttc acgaacgagc tgggcatccc cacctgcacc atcgagaagg aatacgctct
       61 cggcgcggtg ggccagctgc gcacccgctt ccaggcattc atcgaatctc tggagatcaa
      121 acagatacag ggaggcaagt gagatatgaa agcaatggat aagctgatcg cgaagatcga
      181 caaaaagctc gagcgctttg accccatctg gcaggccgaa cacggcaccg gcggccagag
      241 aacgcgctat tacgataaga ccggcgttgc gaagtggcgc gaatggcgcg gcgtgaagga
      301 cacgctctac gactatacgc agtggctcaa taacctctcg agcatggtgc agatggtggc
      361 gtccgctccc gtctcctgtc tcaagggctt ctgggaatac cgctggatgg gctcgtatct
      421 cggcacgttc atgttcatcg accgcctgtt tgagggctac cgcggctatg agctgcgcac
      481 cgcgcacacg aacatgcacg ccatcgtgca gagccttacc aagcgcatcg cgctcgtcct
      541 ctccaacgac aagcgcctcg gcggcggccc gcgcagcgac gatttcctcc cctttgacga
      601 ggtgctgccc ccgctgtttc tgacgggctt caaggagctg atcccgttcc cgctgcagac
      661 gctgccggag ttcatcatct gcgacgtgga ccagcacatc gagccgtact atatcgacgt
      721 ggccgagtcc ttcggtctgg cggcggacgt gtgctcgcgc tgcgccgcgg agaccggcgt
      781 cgctatcgac gatgctttcc ccatcatcgg caaggcgttc ctcacgacga acatgccgtg
      841 caacgcctcc gagtgtacct ccatgatcca gcgccgccgc gtggcgctgc cggacttccc
      901 cgtaacgctc gcgatgatcc acaacgagcc gggcgcgcac ccctattcca cgcagatgct
      961 caaggacgcc attgccttcg tcgagaagga atacggcatc aagtatgact gggacgagct
     1021 gttcaagtac gcccggctta tgaacgagca gaacaccatt gagctcgaaa aatgggacta
     1081 cttcaagacc ccgtattccg cgctcgtcgg cattgccgaa acgctctacc ggctgtattc
     1141 gtgggcgtcc gtcaacggca ccgacccgta tttcaacaag gtcgatcgcc gcgttatcga
     1201 gatcatgcgc cgggcatacg aggaaaagta ccagcctttc ggcggaaaga cgcgccaccg
     1261 cgccttcctt tgggggccct ctgcggtata ctataccgac ttcccgacct ggacacagaa
     1321 ctgctggggc atcaacattg tgctgaacat ggactccacc atgggccaca acatgatcga
     1381 caccgaagac cccgaccagg cgatccagga tctggcgctc ttctccgaga aggccaccat
     1441 gcgccatcac gccgtcggcg gctgggagaa tgtcaacgcc atctgggagt gggcgaagaa
     1501 ctttgactgc gacatggtcc ttttcaacga caacatcgcc tgcaagggca tgctgggcgt
     1561 ccacgctctc atggaggagc aggcgcgcga gctgggcttc cacttcatct tcgtcgagca
     1621 cgatctggag gactgccgca ccgtatcccg gcaggatatg agaaactgcg tcaacaagta
     1681 tatgtccgtc gttctcaacg agaagccgct tgacccgacc atcgtagagt ttgacgactc
     1741 catggcatac taagaaaggc ggaacaggtt atgaagaaat ttctcaagat cctcttcaag
     1801 atcctcggca agctgatcct tgccgccgtg atcttcttcg ccgcaacatt cacgctctat
     1861 atggtcaacg gcgagaacaa gctcatttat tatgttgtgc gtcccctgct gaacaagcat
     1921 tacgattcgc aggtgcgcga ccggcgcatc gtctgacgca ttcaaacgaa cccgtttttg
     1981 ttgccccagg cagatttttc ttctgcctgg ggtattttca taggacgaac cttccgcaca
     2041 tatagatgaa atgccggaag gggagtggtg catgatgcaa acgaacagag acgagcgggc
     2101 gtgtgagctg gcgaggtgga tcgttgagaa caatgccacc gtccgcgcgg cggccaagcg
     2161 tttcggcatc tccaaaagca cagtgcacaa ggatctgtcc gaacgtcttc ggcagatcga
     2221 ccggctgctg tatgaaaagg tgcggctcgt tctcgagcag aacaaggcgg aacggcacct
     2281 gcgcggcggc gacgccaccc gaagaaaata cctgcacgcc gggacgcacg tccccgaaga
     2341 gagaaaaaca cacggctgaa aagtatacaa agcggacggg ctgccgggac tccggcagcc
     2401 cgaaaatcac ttttttccgc ccagaacgaa caccatgctc atcggaacat acagcaccag
     2461 cgcggccacc gaaacgacaa tacccatttt tctttcctcc ttgacaaatt cacatcttct
     2521 ttatgggaca agcataccgc attgtgtgac ccataaaacg gacttccgta atgtgttcat
     2581 aaatcaacac ggtgtcatca aaagaaaaaa accgtaaaaa tcggcgagga acatcttgca
     2641 ttttgtgtcg gttttccgta taatgtgttt gaaactatat ataagggagg gctgaccctt
     2701 gatcgtcaat cagcagaccg ccgcgcagaa aaaggtggac gagctgtatg ccacgctcgg
     2761 acgccgccgc ggagcgtcgc cgttcgggag ctgtccggtc aatctgactg cctcgttcgt
     2821 gcagctgtgt cttgcgcaga gctgcggaaa atgcgttccc tgccgcgtgg ggctggaccg
     2881 gctgcacgcg cttttggaga agatcctcga tggccgcgga acgacggacg atctccgcgc
     2941 cgtgcgccgc agcgccgccg ccatttacga cagtgccgac tgcgccatcg ggtttgaggc
     3001 cgcgcgcctc gtgctcgacg ggctggaggc cttccgggac gattatatgt cccacatcga
     3061 aaacggggtc tgcaccgcgg cgttcgacgc cgtgccgtgc gtggccggct gcccggcgaa
     3121 tgtggacgtg ccgggctaca tcgctctcat ccgcgaggag cgctatgccg acgccgtgcg
     3181 gctcatccgc aaggacaacc cgttccccgg tgtctgcggt ctcatctgtg agcatccgtg
     3241 cgaaaagcac tgccgccgtc agatcgtgga cgattccatc aacatccgcg ggctgaagcg
     3301 ctacgcggtc gagcgcgcgg gcatcgtccc cacgccgccg tgcgcgccgt tcaacggcaa
     3361 aacggtcgcc gtcatcggcg gcggacctgc gggccttacg gcggcgtact atctccagct
     3421 caagggctat cacgtcacgg tgtttgaaaa gcataagcgg ctcggcggca tgctgcgtta
     3481 cggcatcccg agctaccgtc tgccggacga cgatctggac cgcgatatca acgccatcct
     3541 ctccaccggc attcaggtca agctcgatgt ggacatcggc cgggagatca ccttcgatca
     3601 gatccgcagc gagtatgaca gcgtatacat tgccatcggc gcgcagatcg tcaaccgcat
     3661 gaacatcccc gggcaggaca gcgagggcgt gctctccgcg gtcgagctgc tcggcgcggt
     3721 cggcgcgggc gaagcgccgg acttcacggg caagcacgtc gtcgtggtgg gcggcggcaa
     3781 cgtcgccatg gacgcgacgc gcaccgccaa gcgcctgggc gcggcctccg tcacctgtgt
     3841 ttaccgccgc cgcatcgagg acatgaccgc cgcgcgcgag gagatcgacg gcgccatcgc
     3901 cgagggctgt gagatcatgc ccatgctctc cccggtgcgc atcgagaccg aggacgaccg
     3961 cgccgtcggc atcgcggtgc agccgcagct ctccggcccc atcgagaacg gccgcccgaa
     4021 gcccatcaac gccgatcagc cggagatcat tctcccggcg gacgtcgtca ttaccgccat
     4081 cggacaggct atcgactcgg cgcactttga aagcagcggc gtcggcacag agcgccgccg
     4141 catcaaggcc gatccctccg gcgcagtgcc cggcatgccg ggcgtgttca ccggcggcga
     4201 ctgcttcttc ggcccgtcca cggtcgtgcg cgccatcgac agcggcaagg tcgttgcggg
     4261 caacatcgat cactatttcg gcttccatac gccgatcacc tcgggtgtgg atattccccc
     4321 ggcgcagacc atcggcaagc ccgcatgggg ccgcgtcaac atgcgcgagc gcaccgccgg
     4381 cgaccgcaag gaggacttca atctcatgga ggcgtgcctt acggatgagg aggccgcgca
     4441 ggaatgctcg cgctgcctgc gctgcgacca ttacggctac ggcgcattcc gcggagggag
     4501 gaaggacaga tggtaaatct tcgcatcaac gataggcccg tcacggtgga ggacggcacc
     4561 tccattctcc acgcggcgcg ccagctcggc attacgatcc cgtctctctg ctatctgaaa
     4621 cacgtgagca aggtcgcggc ctgccgcgtg tgcatggtgg agatcaaggg taccgaccgt
     4681 cttatggcgg cgtgcgacac gatctgcgcc gagggcatgg aggtctatac gaacacgctg
     4741 cgcgcgctcg tagcgcggcg caacaacgtg cagctcatcc tctcgcagca cgactgcaac
     4801 tgcgccacgt gcgtccgcag cggcaactgc acgctgcaga agctcgccgc ggatatgaac
     4861 atcatcgaga acccctacga gacgaagctc ccggccaaca actggaaccc gaagctcccg
     4921 gtcatccgca ccgcgtcgaa gtgcgtgcag tgcttccgct gcatcgccgt gtgcgagaag
     4981 atccagggca tgagcgtgtg ggactttctc ggcaccggca gccgcgccac cgtcggcgtg
     5041 cgcggcggaa agaagctcga cgaggcgaac tgcgcctact gcggccagtg catcacccac
     5101 tgtccgaccg gcgctctccg cgagcgggac gataccgaaa aggtcatctc cgcgatcgat
     5161 gatccgaaga aggtcgtcgt gctgcaggtc gctccggcgg tgcgcgccgc gtggggcgag
     5221 gggctgggcc ttcccaagga gctcgccacc gaagggcgca tggcggcggc gttccgcgcc
     5281 gcgggtgcca actatgtctt tgacacgaac tttgccgccg acctcacgat catggaggag
     5341 ggcagcgagc ttctctataa aatgaagcat tcgtgggagt tccggcggcc gctcttcacc
     5401 tcgtgctgcc ccggctgggt gcggtttctg aagcttgaat acccnnnnnn nnnnccccga
     5461 tatggtgccg cagctctcca cggccaagtc cccgcagcag atgttcggcg cggtgacgaa
     5521 aacgtggttt gccaaaacgc acaacatcga ccccaaggac attttctgtg tctccgtcat
     5581 gccgtgcgtt gcgaaaaaat acgagtgcgc cgtgccggag atcaacagct ccggcttccg
     5641 ggatgtggac gcggtgctca cgacccgcga ggtggaccgc ctgctgcggc tcaaatggat
     5701 caatgtggcg gatctgcccg aggaagagtt tgactcgcct ctcggcgagg gcacgggcgc
     5761 tgccgttatc ttcggcgcga ccggcggcgt gatggaagcg gcgctgcgca ccgcgtacgc
     5821 cgtgctggaa ggccataacc ccgacccgga cgcttttcag gctgtccgcg gcacggacgg
     5881 gcgcaaggag atctccgcga cgatcggcgg cgtgccggtg cgcgcgtgtg tggcgagcgg
     5941 ccttgcgaac gcccgcgccg ttgtggagga tgtccgcgcc gggcgtgcgc agtacgactt
     6001 cgtggagatc atggcctgtc ccggcggctg cgccggcggc ggcggtcagc cgttccagga
     6061 aggaatggag cttgccggcg agcgcggtga aacgctctac gagatcgacc ggaacaaccc
     6121 ggtgcgcttc tcgcacgaaa acgcctccgt ccagaaggcc tatgacgatt tcttcgataa
     6181 gcccctttcg cacagagccc atgagctgct ccacaccgac cagacaaagt ggagcctccg
     6241 ctaaactttt ttactgtccc ttccgtctgc tataatagac gggagggata tttttatggt
     6301 atcatgcgaa aaaaggaggc ggcgaagcga tggaaacaaa agtaaaacca gccgttgaaa
     6361 catggctctg ccgcgcgtat cttttcttga tgctcgcgct gctgccgctt gccgtccaca
     6421 acggtttttt tgatataacg gaaacgaaaa cgatctgctt taccgtgccg acggtcgtgt
     6481 ttgtccttgc gcgtctcggc ttcacgctct accgggaaaa cggtctcccg cgccgcaggc
     6541 ttgcgggcgc ggaatgggcg gcgctggcgc tctgtcttat atgctttatt gcgtccgccg
     6601 cgggcggcga ttttgccgga tcgttcctcg gcgagcgcgg gcggtatcag ggtctcggca
     6661 tgatgtggct ttatgccgcg ctgtattttg ccttttccgg cgcgcgggtg cgctccggtg
     6721 atgttctcgt cccgctcggt ctcggccttt ttctcagcgg cgcactcacg gtgtgcaacc
     6781 atctcgggta cgacgtgctc ggcttctgct cacagcttgg cgagttcgac cgcgggcggt
     6841 acatatccac gctcggcaac atcaatttcg cgggggcgta tctcacgctc gtgtggccgg
     6901 tctgcgccgc ggcgcttctc acggaaagac ggctctggaa agggatcctt ctggggatcg
     6961 tctgcgtcac cggcctttgg gcggcgatgg cggtgcggag cgagagcgcg gtgctcggta
     7021 tcggcgcggc gctcgtgctg ctgccgctgc ttgcgaaaaa ggagccggac gcgctgcggc
     7081 ggtatccgct gctgctcgcc ggtacagcgt tttccgtgct cgcttaccgg gcggtcgttt
     7141 atgatttcgg aaaacggctc tcctnnnnnn nnnncgtgct gctcaacacc gtgtggagcg
     7201 ccgtgccgct cggcggcatg gacgattggc tgcacttttc ggattcctgg ggcaccgacc
     7261 gtgtcggggt atggaaatac tgcctctcgc tcttcggcga tttcccgttc ggaaaaaagc
     7321 tcatcggcgg cggctgcggc gtgctcgcgg cgctcgatgt gcagcaccgg tatttcccgg
     7381 accccattct cgatgccgcg cactgcgaat atatccagct cctgctcaac tggggcgtgc
     7441 tcggtctggg agcgtatctt gcgtggatcg ttctggcgct gcgccgcgcg tggaaaaagg
     7501 gcggcgcgct cgccttcgct ctcgcggcgg ggcttcttgg ctacggcgtg caggctcttg
     7561 tcaacattgc gcaggcgccc ggtatttcgc tctttttcgt gctgcttgcc gttcttcgcg
     7621 gacaaaatga cgcggaggaa ttgacttgcg gataaatggt gttataattg cgatatgttg
     7681 agaaaaacac atattaagga gtgtgagcat atgaatctga ccccggaaca acaggcaatt
     7741 ctgaacggtg aaaaaggcga agtgcttgcc aaggtgatga aaacgcttgt catgtacggc
     7801 gacgcgttcg gcgcggaaaa gctcgtgtcg gtcacgagca agtacggcca caccgtcatc
     7861 agcttcggca tcaacacgat gaagccggtg tatgacctgt acgaccagct tcttgcctcc
     7921 ggcgtgaaat ttcagcagaa attcaccgcc gacccgcttc cgctcgatcc gaacgtccct
     7981 tcaaatcttt tgcaggatct cgtgttcaag cagttcatgt ataagctcca gccgcgctac
     8041 gaagagcagc tccgccgcat gggcatgaag gacgagaagg acttcacctg cgcctgctac
     8101 tttgacgaag tcggcaacat tcccgaaaag ggcgaggtcc tctcctgggc ggagtcctcc
     8161 gccgttgttt acgccaacag cgtgctcggc gcgcgctgca accgcaactc cggcatcatc
     8221 gacgtgatgg gatcgatgct cggctatgtg ccgtacttcg gcctgctgac ggacgagggc
     8281 cgcaaggcca cctgggtcgt tgaggtcaaa acgacgaaaa agcccgaagc gcagcttctc
     8341 ggcagcgcca tcggcatgaa ggtcatggag gatgtgccat acatccgcgg tcttgatgcg
     8401 tggctcggca cggagctcaa caaggacaac aaggcgtatc tcaaggactt cggcgcggcg
     8461 accgcctcga acggcgcggt cggcctttac catatcgagc atctgacgcc tgaagccgtg
     8521 gagcagggcg acgcgctcat cgcccccggc gcgcaggtct acgtggtaga cgacgccgag
     8581 ctcgagcgcg tgaaggcgtc ctacccggtc atctggaaaa acccgaacgc gaagccgaag
     8641 ctcgccttca tcggctgccc gcatctcacg atggatcagc tcatcgactg gacgaagaag
     8701 ctcgaggaag gtctcaaggc atcgggccgc gacaagctcg ccgtgcccac ggtgctcacc
     8761 accgcgccgc cggtgctcga agagttcaac aaaggcccct atgcctaccg gctcaagaag
     8821 acgggcgcga ttttgagcta tatctgcccg ctgatgtaca tgaacaaccc gctctccaag
     8881 tccatgccgg tcatcacctg ctcgaacaag ctgcgtacct atacctcggc gaagttctat
     8941 tccgaggacg agatcgtcgg catcatcacc ggaaaggaga ccaaataaca tggcgaaaac
     9001 gttcaagggc cgcgttgtcg ttcccggcac cgtcacggcc aaagctctcg tttcccacgg
     9061 cggcttcaac acgcttgcaa gctaccagtc cgcgctcatg tttggcgata aggacgtaaa
     9121 gtgcggcgat cagaacaacc ccgatatcta taaaaagccc atgatcggcg tggcgctgtg
     9181 cctgccgcag accatcggct ccacgaccgg cggcatggtg ttctacaccg ccggcgctct
     9241 cggtaagctg cctgcgtgca tgtgcttctc caagcccatc gactcgcttg ccgccgccgg
     9301 cgccatcctc tccgacgtgt ggacggagac gcatatgccc gttgtcgatt ctctcggcga
     9361 cgagtttctc gaatatgtca agaccggcat gagcgtcacg ctcgccgagg atgggacggt
     9421 gacggtcgaa tgaaatgcgc gatctacggc gccggttctc tcggaacggt gctcggcgct
     9481 tatatgacga agggcggcat ccccgttgaa ctcgtcaacc gcaaccgcgc ccatgtggac
     9541 gcgctgcgcg aaaagggcgc gcacatcacc ggcacggtgg acttctccac gccggtgacg
     9601 gccatcaccc cggaggagat gaccgcgccc tacgacgtca ttttccttat gacaaagcag
     9661 cttcacaaca aggaagtcgt cacgttcctg aagcccctgc tcgcgcccga cggcgtgatc
     9721 gtcacgttcc agaacggcat tcccgagccg ggcatcgccg agatcgtcgg cgagagccat
     9781 acgatcggct gcgtggtcga gtggggcgca acgatggacg ctcccggcga gtgcgtgctc
     9841 acgagcgacc cggacagcct ttcgttccat atgggcggca tgcagggcgt ttcggacgca
     9901 aagctcgcgg aggttcgttc tctgctcgag aaaatgtgcc cggtggcgat ggaggataac
     9961 ctcctcggcg cgcgctggtc gaagctcctc atcaacgcga cgttctccgg tctcggcacc
    10021 gttgtgggcg gcgtgttcgg cgatgtgagt gagaagaagg acgcccggcg cgtcgcggtg
    10081 cgctgcatga aggagtgcat cgacgtcggc cacgctgcgg gcgcgacgtt cgcgcccgta
    10141 cagggcaagg accttacgaa gctgttctat tataaaaacg gcttcaagcg cgccattgcg
    10201 gagctgctcg tccccatcgc aatgaaaaag caccgcgcca ttgagccgtc catgctgcag
    10261 gatctcaaaa agggcaagcc ctgcgagatc gacgcaatca acggcgtcgt gtgcgaatgg
    10321 ggccgcaagt gcggtgttcc tacgcccatc aacgaccgca tcgtcgagat cgtaaaaaag
    10381 gagcaggccg gggaactccc gcttgaggaa aagaatatcc gcttctttga cgatctgctg
    10441 taaaccgaat aagcaaatgc ccctcggcct tccgccgagg ggcatctgta tattgtttat
    10501 atatgcggcg tcgtcatttt tcggcaaaaa actcttcgca aaagcgattc cattttgctt
    10561 catactctgc atgggatacg agcgtcatat ccttcggagt gaagatcccg cgccggttgg
    10621 cttcctctgc gaacgcatag atatagtcac ttgaagtctc gatcacgccg cggacatgat
    10681 cccagccctg cattacggca taataaagcc gggtgtgtcc gtccagagaa atgtatctcc
    10741 cgttataggg aagcaattgc aggacgatat cctccggttt gtgaatgaag gtgcggattg
    10801 ccgcgatttt ttcttcatct acgaagaact gcgacggttg gatattatcc agagagaccg
    10861 tcagacgttc cgtctgtggg agtgccagca ggatgctttt atgcgaatcg taaaacgttg
    10921 tgatatgcgg cgcatagaag cgaaattcct caatggcctt ttggtatgca tgtgggttct
    10981 cccctcggac gattgcttca aaatcggaaa cgatctcaac ctcgcagggt ttaccgtcta
    11041 tgagaaagcc tccatgctga taaagaacgt tttttgcaaa ccgggcgtcg tcataggagt
    11101 ttatccgttg aatttccatt ttcggtctat ccctgctcaa tcggtatagc ggttgatgaa
    11161 ctcggcgcgg atcttggaat agacgatctt ctgctccttg taaaggccga agtttgccga
    11221 gagcatgaag ctgatggcgg cggcgatggc atagtactgg atgtttgccc cgccgaacat
    11281 ctccacgccg aggaatatgg cgctgatcgg gcagttgagc gagccgcaga acatgccgag
    11341 cagccccacc gccgcgccaa gccccggcgc gagaccgaag agatcgccga gcacgcatcc
    11401 ggcggtcgcg ccgatgaaca tggccgggac gatctcgccg cccttgtagc cggtcgtcat
    11461 cgtgagcacg gtgaagagca ttttgagcgc gaacgcctcc gggcgggcat gtccctgcat
    11521 ggcggcggcg atcacgtcca tgcccgcgcc gttataatcc cgcgtgccga gcagcacgga
    11581 gagcagcacg acggcgctgc cgccgagaaa gacacgcagg tagctgttcg ggacggcttt
    11641 gccaagcgct ttcccgccct gccgcagcgc gatgcagtag acgatgctca gcaccgcgca
    11701 cgccgccgcg atagcgacga ccgttccggc gctcgcccac gtaaacggca cggccgcgcc
    11761 gagcgggaac gtctccgccg ctgcgccgag cgcgctggca agatatgccg ccgtgagcga
    11821 cgcggtaagg cacggcagaa tggcggaaaa gtgaaggatg ccgacgcttg cgacctccat
    11881 cgcgaacacc gccgccgtga gcggcgagcc gaagagcgcg gagaacacgc tgctcatgcc
    11941 gcacatgacg atgaggtgta tgtccttttc gtcgagcttg agcgccctgc ccagctgata
    12001 gccgagcacg ccgccgagct gcagcgccgc gccctcgcgt ccggcggagc cgccgaacag
    12061 gtgcgtgatc gtcgttccga cgaaaatgag cggcgcggtc gagccgggga tggaggtcgg
    12121 cgcatggatg gcgagcagca cgccgttcgt gtccgtcggg tgcatgccgc gcagccggta
    12181 aagccatacg atcagcagac cgccggccgg cagaaaatac aaaagccaag ggtatgcgcc
    12241 gcgaagctgc gtagcctcgc ggatggcata gcagaatgcc gtgccgatca gcccgccgag
    12301 cgcgccgatc acaagccccg ccgccgccca caaaacgaac atgcgcacat accggagtgt
    12361 cagcttcgcg tggcggcgga gaaggctttt tctgcccacc gccgtgttct tttcggaatc
    12421 catcgtatgt atctctcttc ctctctggat aaacggcaca gcgcggcagg tttccggcct
    12481 gccgcgcgtt gtgatctttt attcgccccg gagatatttc ttcaatccgg cgaaatcggc
    12541 gctctcgagc gggagcgggt atccgccctt gatatcccag agccggtgcg cgtccgagca
    12601 gcgcagccgg tgaacgctct ccggaatgcc cgcgggctcc gtgtcgtagt agagctccac
    12661 ggcgtccatg tccagatcct ccggccagca gccgagaatg gaatagagcg agttggacgg
    12721 cttatccaca tgcgcgggcc agatcaggcc cgaatactgc cgcacggcgc ggctcagctc
    12781 gttgacggaa atgcgccgcg ccatgaaaag cagatcgcgc tcctccgtcc ggctgccgcg
    12841 cgagccgatc acggtctggc gtccgaagac attcggacgg ttggcaatgc cggggcgcag
    12901 cgtgtcgagc cagcgggaga acgctgccgc cttgccgaga tcgggcagaa ggcagacgca
    12961 gtggatctcc tcggaggtcg tgacctcgat gccggggata aagccgatgc cggccttttc
    13021 cgcctcggca gccacggcgg gacagttgcg cgcggaattg tgatccgtga tggcgatcag
    13081 ctccagcccc gcttccttgg cggctttaac gatcgccgcc ggcgtcatgg tatcgtccgc
    13141 acacggggag agacaggagt gaatgtgaag gtctgtaagg atcatttcgt taccacctga
    13201 aggcggcgga tatccgctcg ccgtaataaa ctgaagaaaa tgctttactt gtctttatcg
    13261 tttttatcgg aaaaaacgca catgcctgcc tcggcccgtc cggcgacgat atcctcggcg
    13321 aaagccctgc attttgggga gccgcacgcg ccgcagtcga tgccgggcag cgactcggtg
    13381 atgcggcgga tctgctccat cttctccatg gcgacggaca cgtccttgtc cagccggagc
    13441 gcggagctcg ccgtgggggt cttttcccag cgcatatcct cgcgggggca gtcgctgggg
    13501 ggcaggggag gggcaaaatc gcgcatcatc tgctggagcc gtgccttcgc aatgaacggg
    13561 ttttccaccg ccagcgcgcc gccgacgcag ccgggatagc aggcgttgag ctcgatgaga
    13621 tcgacgtggg agagcttgcc gtcctcgagc gcttccagaa tgccgatgat gttttcgatg
    13681 ccggaggcgg cgagatggtt cggctcgccc gcggcggacg actgcccgcc ggagatcgcc
    13741 cagctgacgc ccgcggcacc cgcctgagca tagcgctcgt ggtcgtcgat gtcgcccatc
    13801 acggcggtga gacggggcgc gacctcggca atggatacaa cgccggtgat tgccgggcgc
    13861 gccgtgccga gcgcggcctt cgcgcctgcg catttggcgg ggcaggggga gatatatacg
    13921 cacccgatat cctcttccga aagacccgtt tccttcatgg cgagccgctt gctccagcgc
    13981 gccgcgactt ccatcgggga gcggtagttg agcagatgcg gcagcagcga cgggaaccgg
    14041 atgcgcacga tgcgcaggat gaccgggcag tcggtcgtga tgatcgggtg ggggatgctg
    14101 ccgtaattga gctcatagtg cgtcacggcg gacatgacct ccgctgcggc ggcgacctca
    14161 aatacgctgt caaacccgat gcgcagcagc ccctccagaa tgggggcgcg ggtgcgcacg
    14221 ccggggaact gcccgtagag cgcctgcgcg gggagagcga tcgtgtattt gtatttgccg
    14281 agcgcttccg agagcgtgtc caccggcgcc ttcatcgcgt ggcgcggaca gacgcggatg
    14341 cactcgccgc agtctacgca ccgctcgcgc aggatggtcg ctttgccgtc ccggatgcgg
    14401 atcgcctccg tggggcagcg ctgcatgcac ctggtgcagc cgacgcattt ctcgttttca
    14461 agatatatcg aagcgtagcc gatgctcacg atgactcctt tccggcatac accgtgatcg
    14521 aaagggtcgt accctttccg acttcggaat gtatgtcaaa atcatcgctg tattttttta
    14581 tgttgggcag acccataccg gcgccgaagc cgagcgcccg gatgttctgg ggcgccgtgg
    14641 accagcccgc ctgcatggcg agctccacgt tggggatgcc cgggccatgg tcggtgaaaa
    14701 cgaccttgac ggcgtccggc gtgatcgtcg cggtcacgtg accgccgccg gcgtggatga
    14761 ccatgttgat ctccgcctcg tacgtcgtga tggcgacgcg gcggatcatg gcaggagcaa
    14821 agccgagctc cttcagcatg gatttgatat gcccggaggc ttctccggca cgggcaaaat
    14881 cgtcgccggg aacctcatac tcacggacga gcgggctcat tttgcccccg tcaccccatg
    14941 ctgatgcaga atgccgcagg catcatacat ctccagagga gaggtaagca cgcaaacgcc
    15001 gcatttttcc gccagctcca gaatcgaggc ctccggctcc ttgccgcgca ggaatacgac
    15061 gcacgccgta tcgagcattt ccgcggtgcg gatgacctgg ggattgagaa ggccggtgag
    15121 cagaacgtcc ggctgggtgc cgagcgcgag cacgtcgctg agcatatcgc cggcataggc
    15181 ggagatgatc tccgcgtccg catgggccgc ggcggtgcgc gcctcgccgg agagaagggc
    15241 tgtcatttcg ttgattttca tgtttcttgt tctccatctg atcggaagta tccgagtctc
    15301 acattatatt aacaaatatc cgccgcaatt acaaggggaa ttctcggaga aggaatccac
    15361 caagggaaag gaagacgccc ggcgcttttc cggcaaatgc acaaaaaaag aaaaatacga
    15421 aaaatatact ggatttaatt ccatgaatgt gttatattat tttgtcggaa atacccggtt
    15481 ttttcgggca tttttgcgga aattaactgg taaaacatgc aaaaaatgaa atgttatcca
    15541 tcatatgaaa acgcaccgtc cgcaagggga tataatgcgg aaggcacgtg gaatatacaa
    15601 cgttttccgc acaaaatgtc cacgaaccgg ctttcttcgt ccgcgtggtc ccacaggggc
    15661 ctgtaaggag tttgctaaga agcgaaatat aaacaagagg tggaaaggat gtcacggaaa
    15721 aacctgaacg gtgtacatat cccccatcgg aagaacaccg ccgggatgca ggcgatcaag
    15781 atgcctcccc cggcaacggt gaccattccg atgagcatgc acatcggcaa gcccgccaac
    15841 tgcatcgttg cggtcggcga tcacgtcaac gtcggtcaga tgatcggaga accgggcgga
    15901 tttgtttctt ccccggtatt tgcgagcgtt tccggcacgg tcaagaagat cgtccccatg
    15961 ctgcagttta tgggtgcgac ctgccaggcg gtcgtgatcg agtctgacgg tcagatgacc
    16021 gtcgccgaca cggtcaaggc cccggagatc accgattatc aacgccgtgc gcgacagcgg
    16081 cgttgtcggt ctcggcggcg cgacgttccc gacggcggtc aagctggatg tgaaggatac
    16141 ctcccgcatc caggagatcg tcatcaacgg cgcggaatgc gaaggctata tcacgagcga
    16201 ccaccgcacc atgctcgacc gtaccgacga ggtcgtcgag ggctgccgtc tgctcgaaaa
    16261 gtggctggac gtcaagaaga ttatcatcgc cattgaggac aacaagcccg actgcatcga
    16321 aaagatgaag gcggctgcgg cgaatgacga gcacgtcgag gtacgcgctc tgccgtgcat
    16381 gtacccgcag ggcggcgaaa aagttctcat ttaccacacg accggcaaga tcatgccgga
    16441 gggcaagctc cccatcgacg tgggcagcgt tgtgatgaac gtgacgagcg ttgccacgct
    16501 cgcgcattac tgcacgaccg gcatgccgct cgtggaaaag tgcatcaccg ttgacggttc
    16561 cgccatcaag gagccgaaga acgtcatcgc tcccatcggc acggcctgca aggaggttat
    16621 cgacttcgcc ggcggctttg gctgcgagcc gaagaagctc gtcatgggcg gcccgatgat
    16681 gggcgttgcc cagtacgatc tggacgcccc cgtcgccaag ggcacgagcg ccattcttgc
    16741 gttcaacgag aaggacgccc gccccgtgac gcccacggcg tgcatccgct gcggcggctg
    16801 catcgaccac tgcccgatga acctcatgcc cgtggagatc gagcgcgcct atgagaagaa
    16861 cgacgccgaa gcgctcaagg cgctgaaggt cggcctttgc atcgagtgcg gctgctgcgc
    16921 gttccagtgc ccggcccacc gcccgctcgt gcaggtgaac aagctctcca aaacgctcgt
    16981 gcgtgactac gacaaccgca tgaaaactct gaaggaggcg ggaaaatgaa caacggtctt
    17041 atcgtaagcg tctcgcccca ctatcataag cagggcgcaa cgacgcagcg tgtcatgcgc
    17101 gacgtgctca tcgcgctcat cccggcgctc atcgcgtccg tcattatttt cggcttccgc
    17161 tcgctgctgc tcgtggcggt ctccgctgcc aactgcgtgc tgctcgagtt cgtattgaac
    17221 aagctcttca agaaggagca gaccattgcc gatctctctg cggtcgttac cggcgtgctc
    17281 atcgcctaca acgtccccgt gacgatgccc atctggcagc tcctcatcgg cgacttcatc
    17341 gccgtcatca tcgtcaagat gctcttcggc ggtctcggct gcaactttgt gaaccccgcg
    17401 ctcgtcggcc gtcttgccat gatgctctcc ttcaccgaga gcatgacgga ttacctgcac
    17461 acggtgaccg cgcccggcgt tgaccttctc gccggcgcca cgccgctggc agccagcggt
    17521 ctcgacatga gcagctttat gacgctcttc ctcggcaacc acggcggcgt cctcggcgag
    17581 acctgcgctc tggcgcttct catcggcggc gtgtatctcg tggtccgcgg cgtcatcaag
    17641 gtcacgatcc ccgtctgctt cattgcgggc acgttcgtgt tcaccgtcct cttcaacggc
    17701 tttgatgtga ccggcgcttt cctgagcctc ttctccggcg gtctgatgct cggcgccatc
    17761 ttcatggcta ccgactatgt caccagcccg ctcacgacga agggccgcgt gatctttgct
    17821 ctcggctgcg gtttcctcac ctccgcgatc cgcgtatttg ccaactccgc agagggcgtt
    17881 tccttcgccg tgctgctgat gaacctgctg gtgccctaca tcaacgaggg ctgccggaca
    17941 aagcctgtgg gaggtgtcaa ggcgtaatgg aaaagaaaaa tacgttctgg acggaaatgg
    18001 cgcgtcccgt tgttgttctg gtcgttatct gccttgtggc ctccgcgctg ctcggcctca
    18061 cgaacgccaa gaccgcgccc gtgatcgaag aaaacatccg catcgaagcg gaaaagaccc
    18121 gcaaggaagt ccttcccgcg gcttccgagt tcaccgagct tgaggtgagc gaggagctga
    18181 aggcgaacgg tgtcaccggt atttttgaag gcgacaacgg caccggctat gtcgtcaccg
    18241 cggaaaagac cggctacggc ggcgccgtcg tcgttaccgt cggtttcgat accgagggca
    18301 agatcctctc cgtgaaggcc aacgtctcca ccgaaacgca gggcgtcggc agcaaggtcg
    18361 gcgaagcatc catgctcgaa aagtttgaag gactctccgg cgacgtgagc ggcgtcacgc
    18421 tcaagaccgg cgccaccttc acgagcaacg ccgtccgcga tggcgtgcag gccgcttgcg
    18481 ccgccattgc ggcgatccag taagggaggg cggagaaaat gaaaaaaaga atgcagatcc
    18541 tcgttaacgg tattttcaag gaaaaccccg ttctcgttct ggtgctcggc tgctgctcgg
    18601 tgctcgcgat ctccgttacg gtcaagggca gtctcggcat gggcgcggcg ctcacgttcg
    18661 tgctcgtcgg ctcgaacgtc gttatctcgg cgctgcgcaa gatcatcccc gacaagatcc
    18721 gtatcccgtg ctacatcgtc gtcgttgcca cgttcgtaac gctcgtcgaa atggtggtgc
    18781 acgcctatct catcgatctt tataacgcgc tcggcatctt ccttccgctg atcgtcgtta
    18841 actgcatcgt tctcggccgt gccgagatgt tcgccaataa gaacaacgtc ctcgacagcc
    18901 tgtttgacgg tcttggcatg ggcatcggct atacgctggt cctcaccgcg atctccgtcg
    18961 tccgcgagct gctcggctcc ggcagcctgt tcggctatca gatcatcccc gaggagtaca
    19021 ccatccgcat tatctcccag acgccgggcg gattcttcat gttcggcctt gccatggcga
    19081 tgctcgtgct cggcatgtcc aagaagggca agaagttcgt tccggaaatg ggctgcggtg
    19141 acggcggctg cgccggctgc ggcaacgccg aaggctgcac cgagaaagat aaggaggcga
    19201 agaactgatg ctcaaagaag tactgaaaat catgttcggc atgatcttca cgcagaacta
    19261 cgttctcgtg cagttcatcg ccatctgccc gttcctgggc gtttccaaga agtttgattc
    19321 ctgcatcggc atgagcggcg ccgttatctt cgttatgacg ctcgcgagcg ccgttacgca
    19381 tctcatctat cagtacgtca tggtgccgaa cggtctggag tacctctcca cgatcatctt
    19441 catcctggtc atcgccgcgc tcgtgcagtt cgtcgagatc ttcctgaaga agtacgttcc
    19501 gcccctgtac aagtcactcg gcgtttatct cccgctcatc accaccaact gcgcggtgct
    19561 cgccgtgacg ctgctgaacg tcagcaacgg ctacacggct atgggcggcg gcctcctgct
    19621 cagctgcgtc aacggcttct ccgccggtgt cggcttcggt ctcgcgctga tcctcttctg
    19681 cggcgtccgt aaggcgctcg agcatgccaa gcccccgaaa tgctttgaag gtctgcccat
    19741 cacgctcgtg gcggcggctc tcaccagcat gacgttcatg ggctttgccg gcatggctga
    19801 caagctgttc ggttaaggga ggaaagtgac atgtctggag ttataagtgc cattgttgtt
    19861 ctgctcatca tcggcgcggt ggccgcgctg atcctcgtta tcgcttccaa gtacatgagc
    19921 gttcccgaga atgagatgtt ccccgcgatc cgtgaatgtc tgcccggcgc caactgcggc
    19981 gcgtgcggct acgccggctg cgacggctac gccaaggcgc tcgccgaagg cgaagagacg
    20041 agaaccaacc tctgcgtccc cggcgccgac ggcgccgccg ctgcgatcgc cggaatcatg
    20101 ggcgtcgagg cggaggacgt tgtagagatg gtcgcttacg tcaagtgcaa cggcaactgt
    20161 gaaacggcca agcacaagta cgagtacgcc ggcatcaaca actgctcggc ggcgaacatc
    20221 ctcttccagg gcgagtgggc ctgcccgaac agctgcctcg gctacggcga ctgtgagaac
    20281 gcctgcccgt cggatgccat ccatgtcgtg aacggcgttg caaaggtgta tccgggcaac
    20341 tgcaccggct gcggcatctg cacccgcacc tgcccgaacc acatcatcgc gctgcgccgc
    20401 gcgatcgatc cgaccatcgt ccgctgcagc aacacccaga agggcgcgca gacccgcgtc
    20461 gcctgctcga agggctgcat cggctgcatg aagtgcgaga aggtctgccc gaccggagcc
    20521 atcaaggtgg ttgacaatct tgcccgcatc gactacgata agtgtgtcgg ctgcgtcgcc
    20581 tgctcggaga actgcccggt gggtgttatc cagagattct gattttcacc agagaaaatc
    20641 cgtccggcag agccggacgg attttttatg aaaagggttt atccaatgaa gcctgaacgg
    20701 acaaaaaaag accgccttcg cgcggatata ttgcttatcg cggcgctgct cgcactcgcg
    20761 ctcggcttct ttgccgtgca gcgtttgagc cggaaggacg gcgctttggc cgttgtgtat
    20821 gtgaacggcg aacggacggc agaatatcct ctcgccgagg atacgaccgt aacgctgcat
    20881 gcgccgaacg gcggctacaa cattctcgtc atatccggcg gcactgccga cgtgacggag
    20941 gcaagctgcc cggacgggat ctgcgtccgg gcgaaaggcg cgcagtacga gggcgatacg
    21001 atcgtgtgtc tcccgaacaa aacggagatc cgcatcgagg gtgcggcttc agccgatatc
    21061 gatctggtga gctgatatga aaaagacgaa acggcttgtc ctgcttgcca tgctgaccgc
    21121 cgtggcgatg atcctttcgt atgtggagtc gctgctgccg tcggtcggca ttccgggcgt
    21181 taaaatgggt ctggcaaata tcgccgttat atttgcgctc ttccgcttcg gctggaagga
    21241 ggcggcggcg ctctcgcttg tgcgcgtcgt gcttgtaagc cttctgttcg gcagcgtcgg
    21301 cgcgatgctc tattctcttg cgggcgcggt gctgagtctc gcggtcatgg cgctgctgcg
    21361 ccggatcgac cggttttcca ccgtgggcat cagcgtggcg cacaacgccg gacagatcct
    21421 gatggcgatg ctcatattgc agacaaagca gctcctcggc tatctgccgg tgctcgccgt
    21481 ctccggtatt gccggcggcg tgctcacggg gcttgctgcg gcgctgctca tccgccgcat
    21541 tccggaattc tgatttagaa acggtagaga ggtgctcatc catgcaatgc ctgtgccgga
    21601 gaattgattt caggcgatgt ctcgcgtttc tgctgccgcc gcttcttttg atgctgcacc
    21661 agctcctttt ctctgaatat acctccctct atccggacgc gcgcctctat ttgagcattg
    21721 cggacaattt cctgcacacc ggccacttca tccagaccga ccgcgccggt gtggagatcg
    21781 tcgtcccgtt tgcctatccg ctgtatataa cgctcctgcg tgccgtcggg atgccgcttg
    21841 tcggatttac gatgctgaac ctgttgtccg tcggtgcgtc gtccgtgttt ttatacgaca
    21901 ccgagcgccg cctctttggc agcggcggct tttcgtgcat ggcgtatacg gtcatattgc
    21961 tccgcgtcgt gctcccgccg tcgaatctct atgtagaatt cttttatctt ttcatgctct
    22021 gctggctgca atggctttcg ccgaaaaagc ggcttcttct catgaacatc gccgccttcc
    22081 tcgtgttcgc gtcacgcccg gtgctggcgc cgatctatgc tgcggtcgtc ctgtacacgc
    22141 tgtggaagtg cgtaaaagag cggctttccc gcgcgctccc tgtcgcggtc gtggtcgtac
    22201 cgctgctgat ctttgcgttc aacacggcgg tgaactaccg cgagaccgga gagatcatcc
    22261 ccatcagcaa ctacgccgcg gacgccgtct gcttcgcgct caaccccaac agcacgatct
    22321 ggacgagcca gctcaatttc acagcggacg acaccgtgcc ggagctgctg gaaatttatg
    22381 ggaacggcgc gctctcactc tcggaaaaga accgcgcgtt ttatgcgctg acaaggcaat
    22441 tcgttctgca caacacgggg catgtcgtct cggtgacgct caaacgcgcg tggacgatgt
    22501 tcttctactt cacccacggc atgatgacgg cgtcgctcct cggcgcgctg tggatgctgc
    22561 gccggacgcc gcgcggggaa acgcgctcac ttgttctcgc ggagcttgtc ctctccgcgc
    22621 tgctcgtggt catcacctcc gcgggcattt atgaggcgcg gtatatcctg ccggtctggc
    22681 ccgcggcggc gatccacatc gcggccatcc cgcactacgt tctccaatgg tacttcgcgc
    22741 gccgccacgc aaaacagaaa taagcgcata atataagact cgacatcgga aacgatgtcg
    22801 ggtctcactc tgtcgaaaaa gcctcgcaga gtttgcgccc gcaggcgcaa ataaagtttt
    22861 aaccattttc tcc
//
LOCUS       DAWAAE010000017        21813 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1060_length_21813_cov_2.826363, whole genome shotgun sequence.
ACCESSION   DAWAAE010000017 DAWAAE010000000
VERSION     DAWAAE010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1060_length_21813_cov_2.826363"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10007..10016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18045..18054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21148..21157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4282 a         6575 c         6286 g         4640 t
ORIGIN      
        1 ctgccgggct gcgtataggt cttatactgc gccccaccgc ctgcacgttc cttcgtgatc
       61 gcgtcgtatg cctcgttgat ctgcttcatc ttctcctgcg cgagatcggc gagggggttg
      121 ttggcgtagt tgtccgggtg gtatttccgg gcaagctcgc ggtatttctt ttttatctcc
      181 tcatcggatg ccgttcggga aacgcccaga acaacgtaag gatctgtcat tcacttactc
      241 cctgtcggac ggatcagaga tcctccgtat tttttctgtt tttcttccat gttccggcaa
      301 acaccgcatc ggcaacatgc ggaacaccgc aggatagaat gttttcgatg atgggcgtcc
      361 acgcgctctc cggcaggagc gccgcggcgc tcatcgcgcg ggaaagggag agattcagcg
      421 tggcgcgaat ctccgctttt tcctccgccg tcatgacttc ggagccgaag cgtaggcaga
      481 gcgggttgta ccggccgttt ttccgatcgt cggagtaatc gtccgccgcg tccagaatat
      541 atacggcgcg tccgacgtga tagagtatct cgctttgcaa ccgccgctgc atctcgtccg
      601 ccaaccggtc cgccggaaac gccataagac gggcgaagca atccgccgcc tcgtcgagag
      661 acgcgctgcc gcgctcctct actgccgcga gctgagaaag gcactgccgg acgttctcgt
      721 caaatttcgg ctgtcgggcc gccgctttgc ggcagcggct tcgcaggaga cggagagcaa
      781 gtcgggcgcg tattgaccga aagccctttt cgtcggcggc atcatcctgc attttttgat
      841 aagcgaggat gaccgtgtaa tctgcggcgt cgaaaagctc cgtcattccg cagatgcagg
      901 gctgctttcg cagcggatgg actacgcagc ggcgctgctc gatcgccgtc tccggcgata
      961 gcgagagcag cgcgagaaac gtcatgtcgt acccgatgag aaagcgggcc aacaccccgt
     1021 accgctgccg gagcgcctca cagagaccgc agtacgccga tcggaaccgg cggtattcat
     1081 ttatgcgaag ctcgccgata tccggtcgaa tgtagccgta catactctct taatgctttt
     1141 ccttgagcgt gatatagatc ttatattcgc cgacgcagcc cgcttcgggg aagccgtcaa
     1201 tcttgatctc gaccgtcgga gtgaaaacgc cggaggcgtt cgtgccgaga ttggagagat
     1261 ccgccacagc gcggagattt acctcgctga tctggtcaag gacctcctgc ggggcgcgga
     1321 ccgttacgtt aagcgcctgc gtgatgttct ccgcggtata gccgtcagga acgttgatac
     1381 agacgatatt ttccggattc acggagaagg atttcgtagc aacgcccgtg atggatactc
     1441 tcaccgttgc ctcagtgacg ccggtcatat tctcggtgcc gttcggcggg atgatggtgt
     1501 aggtggcgga gaagtccgtg gcgaagctcg aaagattgat cgtgccgaga atgagcttcg
     1561 tcgtgctgtc gatggcggag gcatctcccg cgagcatgat cgtcttcggt tcaatgtcaa
     1621 tgcgggtgtt ggcgtcgcgc gttgcgccgc cgccgtcgat gatcgttacg tcaagcggaa
     1681 cttccttcgt ggaaagcacg ttcagcgtca cggtgacttc ttccgtttca cgctggatgg
     1741 aagagtcttc gatcgggtta tcgtcggaat cgacgagaga atacgtcgta gaatagctca
     1801 gcgtgcggtc cacgtcctca cgcgtaatgg ccacaaacgc atggtcgact ttggagatga
     1861 ccgtcttcgg cccgctgatg gcaacgacca gaggatcgaa caccatgctc tcgtcggcaa
     1921 gatatccgtc cgccgtgttg ccggtgaacg cgcccttgac ctcaatggtc ttgcgggcta
     1981 gcttgtccac atagaaattc acgacgtccg atgacgtgtg gacgagcgtt atttcgttcg
     2041 cgttcacgcc ggcgggatag gcgatatcgt aagaaaccga ataccgtccg tccgccacaa
     2101 cgttgctgag attcaccgtt gccgtcacgt tggagtttgt gagcttggag agcacgcggc
     2161 gcgtgccgga gagcgtcagg ttaacggtgg tgcggtcctg ctcggtgacg atgagatcgc
     2221 tcgacgcgcg catcgcgtcc gcgccgagaa actcgatctt tacgccggtg agcgtttttt
     2281 ccacctcaac gccctcggtc gtcgtcacat acatccagag aagcgacgcc gcaagcagcg
     2341 agaggatcat ccagaaaaca cggctgtccg tgattcgttt gagatccagc tttttccttt
     2401 ctttcgtttc gtttgaatta ttcatcggct ttgtcctttt ctttccggcg gaggaagcgg
     2461 aatgcgcccg tcggttcctc tttcacttcg ggaatcagct cattgcggag gatcttttca
     2521 aaggtgtcct ttgtgagatg ccgcttgagc attccgttga ccgccacgga gatcgcgccg
     2581 gtctcttccg atacgatcac aacaacggcg tctgacgctt cgctcatgcc gatgccggcg
     2641 cggtggcgca tgccgagttc cttgctcagg ttggggttgt tggagagcgg aagcatgcat
     2701 ccagccgcct ggatccgtcc gctgcggatg atcgcagcgc cgtcgtgcag gggcgctttg
     2761 ggatagaaaa tgtttttcag aagctccgcc gtcgtttccg cgtcgaggat cgtgccggtg
     2821 cggatctggt cgtcgagacg gttatcgcgc tcaaagatga tgagcgcacc ggtgcgcgtt
     2881 gcggacatat ccacgcaggc aagcaccgtc tgcgtaatgg cggcgtcggc attcagtgtg
     2941 tgcggcgcgc ccaaaagccg ggagaaaatc gaccctgtgc cgagcttttc caaaaaccgg
     3001 cggatctccg gctggaacag cacgaccagc gcgagaagtc ctacctgcac ggcgttgcgg
     3061 agaatatagt tcacggtgta gagctgcatc tcgccggaca gccagagcgc aaggagtata
     3121 tatacgatac cctgaaacag cttgttcgtc ttcgtgcggc ggaccagcgt gatcaggaaa
     3181 tagaagaacg ctgcgacaag aaggatatcg acgatatcca tcacgcggat cgtgccgaga
     3241 tgaccgaacg cctgtctcag cgatgaccag aaattatcca taggactgtc ccttccttgg
     3301 tttttgctgt gtaaattaca ttatactgga tttaacggat tatagcaaat aattggttac
     3361 aatttgtagc tattttttaa aatattttcg ataacgtccg ccgcgcggtc aatctcccgc
     3421 ggcgtattga acggcgaaac gctcagccgg accgttccgg tttggagcgt gcccgccgta
     3481 tcatgcgcga gcggcgcgca gtgcagcccg gaccggacgg cgatccccgc cgcgccgagc
     3541 cgttcggaga gcgcatcgca gctcatgttc tccggcacga cggagagcac gccggactga
     3601 tcattcttat ccgaataata gacgcgcagc cccggcagaa tgttcagtcg cgcggcaagg
     3661 cggcgcataa gccgctcttc ttcggcataa atgcgctgcg ttgtcctccg tcgtatataa
     3721 cggatgcctt cacgcagacc ggcaatgccg cagacgttgt gcgttccggc ttccagccgt
     3781 tcgggcagcg tttcgggcat aaaatggctg cggctgtcgc cgccggtccc gccggacaaa
     3841 agcggcatcc ccgtgtcccg gcagagcagg atccctgttc cctgcgggcc gagaagtccc
     3901 ttgtgccccg gcatggcggc aaacgccgcg ccgagcctcg gaaaatcgag atccaggata
     3961 ccggcgctct gcgatgcgtc cacaatcagc ggcacagcga atcgccggca gagcgccgcg
     4021 atctcccgca ccggcagaat gaagccgaac acattggaca cgcaggtgca gacggcagcg
     4081 tcacactccg ggagatgccg ctcaaaccag tcgatcagcg ccgctgaatc gaaaagcggc
     4141 gtatccgaag tcagaatgct cgcgccggaa gcgtagagcg gacgcataac agcgttgtgc
     4201 tcaaatccgg acaccgcaac gcgcattcct tcccgcacca gagaatggac ggcaatgttg
     4261 agtgcgtgtg tagcgttcat cgtaaagacc acgttttcgg gctgcgtcat gtgaaagagt
     4321 gccgccgctt cctcccggca agcaaaaacc gtttccgcgg cgcgcatggc ggcggcgtgg
     4381 tcgccgcggc cggggctcgc gcagctctgc atggcgcgta ccacggcgcg cgctacttcc
     4441 ggcggcttct ggagcgatgt cgccgcgctg tccagataga tcacagcggc acctcccagt
     4501 attccccggt aaggcgggag agcagaaacg ccctgccgcg gtcgatgccg cgctcatcga
     4561 gcagcgcgac cgcccggcgt agctttgctt cgttcagccg cacaccgtag ccgcagcctt
     4621 tttcggtgag tccctgcggc gctttcataa cgtacgacgg aattccggcc agacttaacg
     4681 cccggctccc ccgctgcgca tacgtcagcg agcggaacaa aatgatcgca tacataataa
     4741 atccccctgc atacgagatg gcaccccatt ctatgcagag ggaaaaacta tggtttattt
     4801 ctccgagatc aacgccacgg ccatggtgga aatgccctcg ccgcggccgg tgaagccgag
     4861 gtgctcctcc gtcgtagcct tcacgctcac acagtcttcc ggaacaccca ttgcctccgc
     4921 cagacgggaa cgcatctccg gaatgtacgg catgagcttg ggccgctgcg caacgatcgt
     4981 agcatctgta ttaacgatct cccagccgtg agagcgcaga tgtgcgcaca cagcggatag
     5041 gagcttcaga ctgtccgctc cggcgtaccg ctcgtccgta tccggaaata actgcccgat
     5101 atcgccgagc gccaccgcgc cgagcagcgc atccatgatc gcatgggaaa gcacatcggc
     5161 atccgaatga ccaagcagcc ctttgtcata tggaatatcc acgccgccga gaatgagctt
     5221 tcgcccctcg gtcagccggt gaacatcgta accctgtccg atccgcatcc gcttactccc
     5281 tgcgcgtttt taagatcgcc tctgccgtcg ggatatcgac cggtccggtg atcttgatgt
     5341 tttcgggcga gcccttggag agatggactt tcacacccat cgcctccact gcggcgcagt
     5401 cgtccgtata ttctttcttt tccgcgatag cgcgcgtgag agcggccttg atgatctccg
     5461 gaacaaacgc ctgcggcgtc tggatggcgg caagtgcgct gcggtttagc gttcgcacaa
     5521 cggtgtcgtg atcggtctcc ttgaccgtat cggtaacagg cacggccgga gcggcgcatt
     5581 tatagagcgc ggcgctgtgc atcgcatcgt acacgatctc ctccgttaca agcggacgag
     5641 cgccatcgtg gatgagtaca tattttgcat tgcgccggat ctgtacgaga ccggccagcg
     5701 cggattcggt gcgcgaagcg ccgccgacaa cgaccttccc aaccttatcg acacggtatt
     5761 cgcggcagag cgcggcgacc ttttccagag attcaagacg cgttaccaca atgatctcgt
     5821 ccacgcagct gcaggcgttc agcgcggtaa gcgttcttgc aagcaccggc tttccggcga
     5881 gatccataaa gagcttgtcc tcgcccatcc gtgtagaagc tccggctgca acgacaacaa
     5941 cgctgcagga ggtgtttttg gctcggcgaa gaaaatcaga aaagcgactc atcggtttaa
     6001 tacactccgt ttattcggtt tgcgcaggca ctgatccagc tgagcctccg cgaagtcata
     6061 cggacagttc acggagaggg atatctccga gagcaggatc tgccgcgcct gattgagcat
     6121 ccggcgctcg ccggtggata gagggcgttt ttcatcgcgc tgcgtaagcg ccttgattac
     6181 ggcggcaagc tcggtaaggt cgccgctttt aatgcgctgc atgttttcgc ggtagcgctt
     6241 attccagttg tccgccatgg aaacgtccat gtccggaata cgccggagca ggtcgtatgc
     6301 cgtgtccgca tcgacgatcg ggcgcacacc aacctcatca caggcgtttt tcggcaccat
     6361 gacgaccata ccgctcccct gcaacttgag aacgaagtat tcccttgatt ccctgccgat
     6421 acgccggctt tcaatgcgat cgatcacccc tgctccgtga agcggatggg caatgcgatc
     6481 cccgatctgg aaactcatcg cgatcctcct gccttatgcg gcggcacttc cgaaaggtga
     6541 aaaatcctac aaatgtaatt atacacaaaa atgctgattt tgcaaggctt tccggcggca
     6601 gatatatgca aaaaatccct ctgtcaacga cagagggatt ttttagttcg gaattactcc
     6661 acttcggggg cctcgccgga ggcggtgccg tcggaagaaa cggagtagac cagcgcgtcc
     6721 tcgcggggct cgtcctcggt ctcctcgcgg gaggcggcag gagcgggctc ggagagagcg
     6781 cggatcgaca gggagatctt ctgcttctcg ttgtcgatgt tggtgatctt cgcttcgacc
     6841 acatcgccga cgctcagcac ctcggagggc tggccgatgc ggcggttggc gatctgggag
     6901 atgtggatca ggccgtcggt gccgggcagc acttccgcaa aagcgccgaa gggcatcagc
     6961 ttgacgatct tgacctttgc gatatcgccg acggagtagg tctccgtgaa caccttccag
     7021 gggttcaggt tcgggtcgcg gtggccgagg gagattttgt ggttctcctt atcgaagttg
     7081 atgacgtaaa cgtcgatctt gtcgccgacg ttcaggacat ccgaaggctt gttgatgcgg
     7141 ttccagctga tctcagacac gtggaccatg ccgtcgatgc cgccgatatc aacgaacgcg
     7201 ccgtaagagg tcatggactt gaccacgccc tcgtaatgct tgccgacctc gatctcattc
     7261 cagatcttct cggcacgctc cttgcgctcg cggttggcca cggcgcggat ggagccgaca
     7321 acacgcttgc ggccgcggtt gacctcggtg atcttcagac ggacggtctg accgaccagc
     7381 tcggacatgg cggcttcgcg cggcagaccg gtctgggaag cgggaacaaa cacacgcccg
     7441 ccgtgcacgg agacaacgac gccgcccttg ttctcttcgg tgaccttgcc ttcgacaacg
     7501 gtgccgtcgg cggcagcgtt ttcaatgtca gcccaggact tgatggtgtc caggcgcttc
     7561 ttggagagca tcacagtgcc ttccacatcg ttcacgcgca caacgcaggc ctcgatcttg
     7621 tcgccaacgt ggataacgtc ttccaccttg gcagtgccat cctcggtgaa ctcggtcacg
     7681 gggatatagc cggaatgctt ggtgcccaga tcgacggtga cttccgtggg cgtgatgctg
     7741 acaacataac cggagacggt gtctccgtta tatatcgttc tgatggaatc ttccagcagc
     7801 tcatcaaacg tcttttcgga gtcgggcttc ccggcggcat tctccgcagc gggctcggct
     7861 tcaacgtgtg cggtctcttc ggccgcggtc gcagcgactt cctctgccgt ggtctgttct
     7921 accttgatct cgtcgctcat tttttttact acctccttaa tgatccagga cggcaccgag
     7981 gctcccgccg taattccgat acggccctcg cccggcaacc gggatgtgtc cagctccgac
     8041 gcgtcggcga caaagaaaac gctcgcacat ttttcgcggc aaagctctgc cagatgcagg
     8101 ctgttcgcgc tgtgcgcacc gccgatgacg atcattgcgt cgcatatccc ggcaagagag
     8161 gttgcttctc tctgcctctt ggacgttgct ccgcatatag tatcaaatat tttgaaattt
     8221 gtacatcttt tttttaatat tttttctgcc aaaatgtgat ttgcttgaat ttgtgtagtc
     8281 tgaaatacaa cggagagggg tttttcgggc atttccacgg acgtcgagag ccattcgtcc
     8341 agcgccgcgg cgtccggcag cacgacagcg ccggtacacc agccgcaaat gccccgaatc
     8401 tccgggtgct ccgcgtcgcc gatgacaacg atctgccgcc ctttttcgga ttcttcacga
     8461 acgatcttat ggatgcgctc gaccagcgga caggtcgcgt caatcactcc ggcgcggcgg
     8521 tttttcagtt cgtcatatac cgcgcgggaa acaccgtggg cacggataat gacccgttcc
     8581 ccctccggca gctccgaaac cttctcgatg accgaaagcc cttttttctc cagcgcggtc
     8641 acggcgtcgc ggttgtggat cagctcaccg agacaccggc acgcgccgga agcaagcgct
     8701 tcctccgcca tgcggatcga ccgctctacg ccgaagcaga atcccgcgct gcgggccaga
     8761 atatactttg ctgccacgcg ctcattcctc cggctttagt gcccagatgc gttccatcag
     8821 ctcgcgcgcc agccggtcat aatcacggtg gtcgcccaac tccggataat acggctcgcc
     8881 gatcacggtc ggcacctcgt gaaagatctt cttgtcgcgc gggatatata ccggtacgat
     8941 cggcacatgc atacgcacag ccatatggat cacgccggat ttcggttcta cgacggcatc
     9001 tccgtgcacg cgcgtcccct ccgggaacac gatgagcttc tcccctgcct tgagcacacg
     9061 catgcactgc ttgaaggaat caatgtcgcg ctcgccgcgc ttgacgaaga tcgacccggc
     9121 ttttttcatg agccagccga aaataaacat cgttttcgac tccgccttgg caaggtgatg
     9181 cacataggtc ttgcgtccca gcgcgaagga aatgaggatc gggtcggcga cattggaatg
     9241 cggtgcgcaa aggatcgccg gcccctccgg aatattttcc agcccataaa atttctgcgg
     9301 atacagcagc gccccgatgg gacgaagaac atcataaaca agacggtacc atttttcttt
     9361 tgtcatagtg caaaacgctc ccgaatcaag gattccaccg ccgcgcagct ctcgtcaaaa
     9421 tcgagctgcg tcgtatccag acgcaccgca tcctccgccg ggcggagagg cgccgcagcg
     9481 cgggaggaat cgtttttgtc gcggtattcc atatcgcgca gtacttcttc aaaggggagg
     9541 ctcttcccct tcttctccaa ctccagctgg cggcgtttgg cgcgcgcctc cacggacgcc
     9601 gtaagaaaga tcttcagccc cgcgtccggc agcacaacgg tgccaatatc gcgtccgtcc
     9661 atgatgacgt tctgcgtgcg tgcaaacgaa cgctgcatct ccatgagaaa cgcgcggact
     9721 tccggaacag cggacacgtc ggaggcataa atggatatct ccggcatgcg gatggcatcc
     9781 gaaacatctt ccccgtcaag aaacatccgc tggagaccgt cggcgccgtg gcgcagttca
     9841 atgtgcagct ccggcagcat ggcaatgacc gcggcgctgt ccttggaggc gatgccgcgc
     9901 cggtacgccg cgagaccgac cgtgcggtag atagcgccgg tatctacata gataaaaccg
     9961 aatttttccg cgcttttgcg ggcaatcgtg ctcttgccag cgccggnnnn nnnnnncaat
    10021 ggatatgggt ttcattccgt aatctccttt tccggtatcg cccgtccggc cgcatagccg
    10081 gtgctccagg cgatctgcag attgtatccg ccggtgcagg cgtccagatc aaggatctcc
    10141 ccggcaaaat acagtccgga aacgatcttc gattccatag tgcggggatt cacctcccgc
    10201 acatccacgc ctccggcggt cacgatggct tcctccaccg ggcggcgggc gatgagccgg
    10261 atcgtaaagc gcttgatcgt atcgcgcagc gcgagacgct gctcacggcg gatgctgttc
    10321 accttgagcc gtgccgggat gccggatcgc tcgatcacga ccgggatgag cttctgcggc
    10381 aggagagcgt cgagcgcgtt cgtaaaatcg cgattgatgt tctcggaaaa gtcccgcagc
    10441 agccgctcat cgagcttttc cgccgtgagc cccggcttga gatcgatctc cagacggcag
    10501 gaggagactt tttccggaat atgagcgctc gccgaaagca cgagcggccc cgatacgccg
    10561 aaatgtgtga acagcatttc cccctgctcc cggtacagga ccttatcgcc gcagaaaagc
    10621 gtcagtccga cgttccgcaa agaaagcccc tgcatccggg cgcagtctgt ctccgcctcc
    10681 aacggcacga gtgacgcgcg cgtcggaacg atggtgtggc ccagcttcga ggcaatgcgg
    10741 taaccgtcgc cggtggagcc ggtcgcggga tacgataagc cgccggtgca taaaacgacc
    10801 gcccggcagg gatattcatc gcggtctgtt accgctgcgg aaacgtgtcc gctctctgtc
    10861 cggatgtcga caagacgttc ccggacaaag cgaacgccgg cgctttttgc catgcgctcc
    10921 agcgtatcgg caatgtcgtt ggcgttatcg gaaacgggaa acacgcgctc gccgcgctcg
    10981 gttttgagcg gcacgcccgc ctcttcaaaa aaaaccttca cggcctccgg cggaaaggcg
    11041 tacagcgagc ttttgaggaa gcgcgcattg gtgcacacct tctgcagaaa ttcctgcggc
    11101 gcgctgtcat tcgtaacatt acaccggccc tttccggtga tgcggagctt cttccccgtt
    11161 tttccgttcg gctcgaggac cgttaccgaa agtccgcgcc gtgccgccgt gatcgccgtc
    11221 ataagcccgg cggctccggc gccgacaatt atcacatcag tcattgagat tttcgtatac
    11281 ctcatattcg ctccagatcc cttcgagctt ccggaacgta tcggataggt tgtcgggacg
    11341 ctggtagccg atggcaacaa tgagcgcatt gatgagactc atcggcgcta caagcgaatc
    11401 caggaacgaa accatatcgc tcttggcaaa aagatttata tccgccacgc cgacgaacgg
    11461 cgaggactgg ccgtccgtaa tgccgatcgc cgttgcgccg gtggatttgg caaactgcat
    11521 tgccttgatc gtgcgcgagg aatagcgcgg gaagctgatg ccgagaaaaa cgtccccttc
    11581 atggatgcgg aacacctgct catagacctc gctcacggac gtatcatgga tcagccgcac
    11641 attgtcgagc agaagattga gataaaaccc gagaaagctc gcaagcgatg tggacgagcg
    11701 catgccgacg atataaacgg tctcggcctt gaggatcgca tcgaccgcgc ggttgaagtc
    11761 ttctttgctc acctcgtcga tcgtgctgcg gagcttgtcc atatccgcgg agagcacggc
    11821 gtcgagaatg ttgccgcggt cgatctgttc cttagcgacc tcaatgcgct gcaccgccgt
    11881 aagacggttg cggatcatct cctgcatggc gcgccgcata cccgggtacc cgtcataccc
    11941 aagctccgta gcaaagcgga cgaccgtcga ttccgaaacg ccgaccgtct gcccgagctt
    12001 tcccgccgtc ataaaggcgg ctttgtcgta gttttccgaa atgtaccggc agatcatccg
    12061 ctggcccttg gaaaatttat gattctcttt attgataagc gccagaatat cgcgttccat
    12121 ggcaggcccc tcataattcc tttacaaaaa atctcccgga tgatatccgg gagtttcatt
    12181 ttagacctat tttcctgcaa atgcaagcta attttgcaat tcatgttgac aaatcctgca
    12241 aaaagaagcc atcagcgcag actttgcaag tattccagct cttttgccgt caaataccgc
    12301 cactgtccgg cgggcagatc gccaagggaa atctccccct gccggatgcg gagaagccgc
    12361 tgtaccttga gccccgcggc ggcgcacatg tttcgcacct cgcggttttt cccctcgcgg
    12421 atcgtgacat cgataagcgc ccgatcctcc gtggagcgaa gaacgcgcac ctccgccgcg
    12481 cggtaccgca ccccctcata tacgatcggg cggcgcagcg ctccggcggc ttccgccgcg
    12541 tccgggccgc tcacccatac gcggtatgtt ttgctcacgc cgtgcgacgg atgcgccaga
    12601 cgattcgcca tctcgccgtc atcggtgaag agcagcagtc cttccgacat gcagtcgagc
    12661 cgaccgaccg ggaaaaggcg cacaccggcg tcctgcacaa gctcggcgac cgtcgggcgg
    12721 ccgtgcgtgt cgcgcagcgt tgtaacgtat cctcggggct tgtggagaag aacatatgta
    12781 tgctcctccg gcagcgaaag acgcttgccg ttcaatatga caacgtcgcc ttcttctgcg
    12841 cccgatccga gagaggcggt cacgccgttg accgtgactc tcccctgccg gatatattcc
    12901 tccgcctcgc ggcgggagca aacgcccgcc gcggcaatgt atttttgaag tctcattctc
    12961 tttcttcctt tgctgatcgg ttatgccggg tatatgccga aaaaaaacga ttcacgcgcg
    13021 cttttctccc cggcccatcg gagcgccgcc gtacacacgg cctcttcatc cgcccaaata
    13081 acaagttcac ccgctgcggc ccccgccgga accggcgcaa aaataaaatg cggcagacgg
    13141 acggcaagct caacttccgc gctcttcgga aggcacaggc aaacgctgca gtcgagcacg
    13201 gcagcatttt ccgcttccgg accgccaatg accggcacat gctccaccgg cttcccttcc
    13261 gggagtctga actccgcgta ggcatcaaac gcccagtcgt agagcgcggc atgatccttc
    13321 cagtcgttcg ggtcggaaag cgttacgcag atgagctgca taccgtcgcg cttacagctc
    13381 gtgacgaggc atcggcccgc ggctttggtg tacccggtct tgccgccgtt cacgccgcgg
    13441 cagttccaga gcagcttgtt gtggttcaca tacgtcaaac cgtggataca gctggtgcgc
    13501 atggagagga tctccgcgag aacgtcgttt tgaagcgccg ccgccataat gaggccgaga
    13561 tcgtgcgcgg aggcgcggtg tccgtcggca tcaagcccgt gaggattggc aaaatgtgag
    13621 ccggcaaggc caagttcctc cgctttgcgg ttcatctgct caatgaacgc atcgccgccg
    13681 agcgaaccgg cgatagcgag cgcggcgtcg ttgccggagg cgagcagcag cccctctaaa
    13741 agctcgcgga gcgtatagct ctccccggca cgaaggtaca tggacgagcc ctctaccgtt
    13801 gtccactcgc tctgcaccgt aacgcggtgt tccggcgagg cgagatcgag cgcggtaagc
    13861 gccgtcataa gttttgttgt gctcgcaatg agcatcggcg tatcggcatt tttctctgca
    13921 agtaccgcac cggatcctgc gtggagcacg atcatcgctt ccgccgaagt gccgggcggc
    13981 gagaataccg ccggagcgga ggcggagaac aggatcgccg ctgccagagc cagaattacc
    14041 gttttcttca taaataagca ccacccatgg attgattcgg gcggtgctta gtatgaccgg
    14101 tatgccgggg aaattattcc ttgttcttct tgtttacgat gttgtcgatg cggtcgagca
    14161 catcgggcat catctccaca acgcgatcca tcgtcgaaag cggattggag gaaacgctga
    14221 tcatgcggca ggtgccgtcc ttgatgacaa agaagccgac cggctcgacc ttgacgcccg
    14281 cgccgttgcc gccggtaaag gaaatgccct tgctgccggt cgtgccgccg gtgccgatgc
    14341 cgaagctcat gcgcgagacc ggaatgacgg taatgccgtc gggcgtgctg atgggtttgc
    14401 cgataatggt gtctgcgtca accagctgcc ggatcttact gaccgtcatg tccatgagag
    14461 cgttgatctt attctcttcc attgtcgtct ttcctttcat tcgtcgccgc ggcaggttta
    14521 tcctccggac ggctgtttat tttccatttc aagaactcca cgccgagcgc cacggccaga
    14581 tgcaccagcc ggaacagctg cagcgagaga acgatgcgcg cagagaccgt cggctcttcc
    14641 gatgtatagt ccgcgccgat ctcgatgtca cgccggagaa cacggatctt ctttcccgcc
    14701 agcgccggga gcgtctccgc cgcggcgcac gccatgccgt actggatcgc cgtatcatag
    14761 gggtcccggc aggcgacggt atagtgcagc tggaagaagt tcacggtgaa gctgcggaaa
    14821 aagcggcgga tcgcatgcga gcccatccgg agcagggcaa cgatggtgtc aaagtcgagc
    14881 ttgggcttcg gcttgacggt cacgctgtcg ttgatctcct tttccttggc agcggcggcc
    14941 ttctttgcgg ccttggcagc gcggcgcgct tcctttttgg ctttttttgc ctcggctttc
    15001 agccgctgct tttccgtgag cggctttttc ggcagcagcc agatgcgcag cggcccggcc
    15061 ttgatcccca cgcgcagcac ctcatccgca tagccaacgt ccacgccgta cggcaggaaa
    15121 aacacaagca ggatcaaaat cgccagtacc gccagtatga tccatccaac caatcacatc
    15181 acctcgtccg gagtcattcg atctccgtga ttttgagctg ttcgtcttta tcgcttgccg
    15241 cgtcaatggc ggcctggagt tcctgtattc cctcgcccgt ggcgacattc ggcagcggcg
    15301 gcagctcctc gatagaggaa atgccgacaa cgcggagaaa ctgatcggtc gtcccgaaga
    15361 gaaccggccg tcccggtgcc tccagacgtc cgcagggagc gatcagccca cgttcggaga
    15421 ggagcgcgac ggtataggag ctgtcggtac cgcgaagctg ctctatgtaa gagcgtgtca
    15481 cgggctgata gtacgcgacg atcgccaaaa cctccagcgc cgtcggcgag agcttcggct
    15541 gcgcgcgctt ttcgagcgtg cggctgacca cgtcgctgta ttcgcccgcc gtaacgagct
    15601 gcagccggtt ttccattctt gccagacgaa aaccgcggcc gttttcctcg tattctctgg
    15661 caagctgttc cgcagcctgc cggacgtccc acggctccgc gccgaggacc ttggcaatgc
    15721 gttctgtttc cacgctgtct ccggcggcga agaggatcgc ttccagcgcc gggcggatct
    15781 cattgattcc taccatttat tcttccgtat ccgtgagcgc acggctcaca tcgtaatttc
    15841 cttcttcccc gctgacgcgg atatgcccgt cggagcagag ctccagcacg gacagaaatg
    15901 ttgcaacaag ctcgctgcgg cttttgcaga tgccgaacat ggagccaagc gacatatgcc
    15961 cgtttttccg gagaaggccg agcagctgtt cgctcttttc ccgaacgctg aatatgatcg
    16021 gcttcggcgc ggcacggcgc agcgtttcat tttcctccgg agccgattcg ccggtgccgc
    16081 ggagcaggat ctgcgcgagc gcgctgagca gttccgccgg cgtatgacgg tagtcatacg
    16141 gacgttccgg caaaggcagc gggccgcggg tctgcatttt catgcccacc tcggcaagct
    16201 gtccgatctc cggggcgatc tcccgcacgg cggcgatcgc atcacggttt ttgagctgct
    16261 cgagcgctga gacgagcact tcgagttccg tcggctcttt ctcctccgtc aggagcattt
    16321 tggttttgat gtacatgaga tgcgacgcca tctggacaaa ctcgctcgcg atttccagat
    16381 ccatctcctg catcttctga agataggcca gatactggtc aagcagctgg gagatctgga
    16441 tatcgcgtat ttcgatctta tccttggcga gcagcatcag tatgaggtcg agcgggccgg
    16501 taaaatcctc cagctcgtcg cgcgcatgca cgacgccttc cagatgatag ctcggttctt
    16561 ggatcatggg tataccgtat gcctttacac gaaatgcgtc acaagccgaa agccgagttc
    16621 ggcaaagacg gaaaatttgt caaagagcca ggaggtcgcc gtggagagcg gcccgccgag
    16681 aatctttgtg gagaccagta cgaggagcaa tatcatgcca taacgctcgt agcgcatgag
    16741 cttgaaatag ctctcatccg gaatgaagga gaaaagcacc ttgctcccgt caagcggcgg
    16801 gatcgggatg aggttgaaca ccgccagcgc gaggctgagg tatgccgttg tataaacgag
    16861 ctgaagaacc gtttcggcga ccgctccccc gtgaacatac agcgggtaat acagcaggcc
    16921 gtacaggaag agaaagagca gcgcgagcag aaaattgcac gccggacccg ccagcgccgt
    16981 caccgccatg ccctttttcg ggtttttgaa ccggtacatg ttgaccggca ccggctttgc
    17041 ccagccgaaa cgaaacacca ccatcatgag aagacccatg acgtcgatgt gctttatggg
    17101 gttgagcgtc aaccggccgg catcctttgc cgtcgtgtcg ccgagccggt aggccacata
    17161 cccgtggctc agctcgtgca gcgtgatgca caaaagcgat gggaaaatat tcaccacgat
    17221 ctgcaccgga accgaccagt ccagattctt ccagatattt acaaagtcac tcatagataa
    17281 gttaatatta ccaaaaacta cggcgaatta caacagcaaa tacaacacta aatattattt
    17341 tcgatgaatc cgagcagctc tcggcggccg acctttgccg tgcaggaata cagaagcagc
    17401 ggaaaatcgt cgtccagccc cagcacgttc cgtatacgct cgagatttgg ctcgatctca
    17461 ctttttttca gcttgtcgca cttgtttgcc acaacgacga acggtcggcc ggcatcgaag
    17521 aaatactccg ccatcgtcac atcgtccgcc gtgggcgcat ggcgggaatc gacgatcagg
    17581 acacccatgg taaagaggtt cggctcctga aagaaatctt ccataagcgc gccccagcgg
    17641 cggcgctcgt cgccggaaac gcgggcgtag ccgtagccgg gaaggtcgat gaaatatgcc
    17701 ccgtccacga ggaaatagtt gacctgcacc gtctttcccg gcgtcgcgcc gacacgggcg
    17761 tagtttttcc ggttgagcag actattgatg accgaagatt tgccgacgtt cgaacgcccg
    17821 gcgaacgcga cggcatggcg gccgtcgcgc agaaacgcct ttttgttcgc ggcggatttt
    17881 atgaactcta ctttctgtgt attcattccg ccaccttctc ccccgccggg cggagcgcct
    17941 ttttcagcac ctgatccatg tgggaaaccg ccgtaaagtt tacggcgttg cgaacgttcg
    18001 gatccatctc ggcaagatcg ctcacattgt cctccggcag aagcnnnnnn nnnncacctc
    18061 gtggatcccg gcgcggagcg ctgccatcgt cttttcctga atgccgccga tcggcagcac
    18121 gcggccgcgc agcgtgatct caccggtcat ggcgatatcg gcgcggaccg gacggttcgt
    18181 aagcgcggaa acgacgcaca agcagatcgc ggcgccggca ctcgggccgt ccttcggcac
    18241 ggcgccttcc gggaaatgca gatgaatatc ggtattctta tggaaagccg gatcaatgcc
    18301 gagctcggaa gtgcggctgc ggatgtacgt taccgccgcc tggcacgatt ccttcatcac
    18361 atcgccgaga ttgccggtca gctcaagctt tccgctgccc ggcacgacgg agcactccac
    18421 atcaagcacc tcgccgccgg cgctcgtcca cgcaaggccg tgaacaagac ctacggaatc
    18481 ggtcgagaag ttgacctccg gcttgaattt gacggggccg agccacggct cgagctcgcc
    18541 cgcgcgcagc cgcagagacc ttctctcccc ggaggcaatg ccgcgggccg ccttccggca
    18601 gagcgcggcc agctcgcgct caagcatgcg cacgccagac tcgcgggtat agagcgctat
    18661 gatctcgcgg atggcatcgt ccgaaacacg cagctggttt ccggaaaggc cgtgcttcgt
    18721 ccgctgcttc ggcagcagat gctgcttggc gatctgcagc ttttcctcgt cggtatagct
    18781 gccgagggaa atgacctcca tacggtcgag cagggcgcgg gggatggtgt cggcggtgtt
    18841 ggccgtcgtg atgaacaaga cctcggagag gtcgaacggg atctccagaa aatgatcgcg
    18901 gaaggagccg ttctgctcgc cgtcaagcgc ttcgagcagc gcggcggacg ggtcgccccg
    18961 gtagtccgag ccgagcttat cgatctcgtc gagcacgagc acgggattcc gggaaccggc
    19021 ctgctgaatg ccggcgatga tgcgccccgg catcgcgccg acataggttt tccggtggcc
    19081 gcggatctcc gcttcgtcgt gcacgccgcc gagggagatg cgggcgcatt tccggtttgt
    19141 agcgcgggca atgctcatgg cgatgctcgt tttgccgacg cccggaggcc ctacgagaca
    19201 gagcaccccg cccttgacct gcgcggagag ctgccgtacg gcaagatact ccaatatacg
    19261 gtccttgacc ttcgtaagac cgtagtgatc ctcatccaga acctttttcg cgtgggcgat
    19321 atcggtgacg tccttcgtcc tctcgttcca cggaagctcg agacagacgt caagataggt
    19381 ccgcagcacc gcgccttccg ccgacccgaa cggctgcttg gcgagacggg agagctcctt
    19441 gagaagcttt ttctcgatct ccgcatcgag tccgagatca aggatcttcc ggcggtattc
    19501 gtcgagatcg tccgtttcgg acgcttcccc cagctcctcc tggatcacgc gcatctgctc
    19561 gcgcaggaaa tagtccttct gatttttcgc cacgcgctcc gccgccgtgg actggagatt
    19621 ctgctggata tccatgatct ccagctcgcg gtgcagcata tcgcagagca tggtgagccg
    19681 ctgaagcggt gagcactctt ccagaagcag ctgcttttcc gccggctgca gaaagacatt
    19741 ctgggcaatg taatccgaga cgaagcccgg ctccttccgg tccgctacac gaagcagagt
    19801 ctccggtccg atcgttcccg acgcggaggc atactcggca aagaggtcgt tgcacgatcg
    19861 gataagcgcc gtgcattcga gcgtgtccgc atcgaccggc ttatcggcaa gcacccggac
    19921 atttgcccaa agcgccgggg tctccgccgt gaggcgcagg agcttcgcgc ggtatagccc
    19981 gtcaacgatg catttgacgt ttccgccgcc gggcgctcgc aggatctggc tcaggcggca
    20041 gatcgtgccg accgtgtaga gatcgtctgc gcgtggcgtg tcgcaggcga tgtcacgctg
    20101 cgctacaaga aagatcatcc ggtcggtgcc gatggcggcg ttgagcgcgg caatgctcat
    20161 cgggcgctcc acatcaaagt gcagcaccgt tccgggaaat actgaaacgc cgcggagcgc
    20221 gagcgtcggc aatgtcatga ttcggctggt attttcgctc atatgttgtc ctccatagca
    20281 aacgagcggg gacgtgcccc gctgtctgcc atgtgttccg tttggttaca cactgtctga
    20341 attatcgttc cgcagcgcta cgggcggctt gccgtccttc acgcagtcag cggtgatgat
    20401 gacgcgcttg atgctcttgt ccgaggggac acggaacatg atatcggtca tgatcttttc
    20461 gagaatgctg cgcaggccgc gcgcgccgat ctccatttcg agcgccttat cggcgatggc
    20521 ctcgatggct gcgtggtcaa actcaagctt gacgccgtcg agcgccatga gcgctgtata
    20581 ctgcttggta atggcgtttt tcggctcggt gaggatgcgc tcaagcgctt ccctgtcgag
    20641 cgagttgagc ggcgtaatga ccggcagacg gccgaccagc tcggggatga tgccatactt
    20701 gataacatcg tgctgctgcg cctgcgcgag gatctcgccg aggttctttt ccttcttgct
    20761 cgtgatctcc gcgccgaaac ccatgctctt gcggtccatg cgattttcga tgatcttgtc
    20821 cagaccgtcg aacgcgccgc cgcaaatgaa aagaatgttc gtcgtatcga tgtggatgaa
    20881 ctcctgctgc gggtgcttgc gtccgccctg cggcgggacc gcggcgatcg tcccttccag
    20941 gatcttcagc agcgcctgct gcacaccctc gccggagaca tcgcgcgtga tcgaaacgtt
    21001 ctcgcttttg cgggatatct tgtccagctc gtcgatgtag atgatgccgc gctgggcgcg
    21061 ttccacatca aaatccgccg cctgcagaag gcggagaagg atgttctcca catcttcgcc
    21121 gacatagccc gcctcggtga gcgtggtnnn nnnnnnnggc acatccagca cgcgcgcgag
    21181 cgtctgcgca aagagcgttt tgccgctgcc ggtggggccg atgagcagaa tgttggactt
    21241 ctgcagttcc acatcggact gctggttgta ataaatgcgc ttatagtggt tgtaaacggc
    21301 gacggaaagc gcgatcttcg ccgtctcctg cccgacgatg tattcgtcga ggaatttctt
    21361 gatctctgcc ggcttcggaa ggtcgtccac ggacggcgcg ctcggcgcgc tctttcccgg
    21421 atcggtgcgc tgcggcaggt cctcatccac gatgttcatg caaagacgca cgcagtcgct
    21481 gcagatgtag gcgccgttgc cggcgatcag cttgtccacc tgatcctgtg tccgaccgca
    21541 gaaagcgcag cggatctttc tatcgtctga tcttgccatt cgatctccct gaattatctt
    21601 ttataaatga ccttgtcgat gaggccgtat tcgcgcgcct cttccgccag aagccagtgg
    21661 tcgcgctcgg agtcggcttt gatctgctcc ggcgttttgc cggtgttctc cgccatgatc
    21721 cggttcatgc gcgccttgat cttgagaaaa cgttccacat cgatctccat atcagtgacc
    21781 ttgccctgcg ttccggcggc gggctggtgg atc
//
LOCUS       DAWAAE010000018        20740 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1142_length_20740_cov_3.921537, whole genome shotgun sequence.
ACCESSION   DAWAAE010000018 DAWAAE010000000
VERSION     DAWAAE010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_20740_cov_3.921537"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10964..10973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4124 a         6263 c         6449 g         3894 t
ORIGIN      
        1 aaaaagcacc gaaaagactg gctgtccgac gtgtgtgctc ttgttaaccg acgctttcaa
       61 atgatgaaaa gacccgcgga cagaagcgat aacacggttc tgtccgcgag tctggtcatt
      121 tcatgcaaac caggcggtag acccgccgag tactgttgac ttgcagccgc gcatggcggc
      181 agactactcc ccctcggagt gcctgtattc tatcgtggaa acggcgcgct gtcaagggac
      241 agaaagccgg tttccgccga aaaattcggc ttcggcggcg tcgtgggtga caagtaaaac
      301 ggttttgtca tcggagacgg cgcgcaccgc gtccatggcg gcgcggcggt ttgcctcgtc
      361 cagccccttg aacggctcat cgagcacaag cagatcaacc tccgcgcaga gagagcgcgc
      421 gagcgcgcac cgccgccgca tcccgccgga gagctccgcg gcgggaacgg cgagactctc
      481 gccaaggccg agccgcgcca gtatatcgcg cgcctcgcgc tcctttttcc gtccggcggt
      541 gagcagaacg ttttcgagcg ccgtgagctg cgggcagagc cgatcctcct gaaataccgc
      601 ggcgcggcgc gccggaacac cggcgatctc gcctccgtcc ggcgtttcaa gaccgaggat
      661 gagccgcagc agcgtcgttt tcccgccgcc ggaaacgccg gtgatgacgg tcgtttcgcc
      721 gagcgggagc ctagccgaaa aatcgcggaa aacgaccgtt tcgccgtagc tttttgaaag
      781 atttgttatc aaaatggcgt tcgtcatacc gcgtgctcca gagcatcaaa gattttgccg
      841 agaagaaaga gaaatacgcg ctcaaaaaga gcgctcaccg cgacgatcac gaccgtccac
      901 gcgaaaagat cggcgatctc cagatacgct ttggcgtcat agagcttgcc gccgacgctt
      961 cgcgccacaa cgccgatgac ctccgccgcg acgcccgctt tccagcagag cccgagcgcg
     1021 acgcggcagc ccgcgaacag ggcggggcgc agcgccggaa ggaaaatgca ccggacgcgc
     1081 cggcccggag agagacggaa cacgtccgcc atttccagaa ggtttcggtc ggcggcgcgg
     1141 ataccttgca gcagattcgt atacaatacc ggaaagacca tcagaaaggc gataaacgtc
     1201 gaaagccgcc gcgccgagag ccacagaaac gcgatcacga tgaacgacgc gaccggcacc
     1261 gactggatcg ccgcgacgta gggccgcagc agcgtttcga taaacggcac ggcggcggag
     1321 agcgtggcaa ggatccccgc gagcgcgagc gcgagcagaa agccgagcga aatgcgcgaa
     1381 aacgtgaagc cgagcgtcgc ccggaagtcc gccgtaacga taagctcaca gagccgccgc
     1441 gcgacagcga cgggcgttac gagcagaagc tcgttcccga gcgccgccgc gcccgcctgc
     1501 cagacgcaaa gcgcgagagc cgcggcgaaa aatctcgccg cggcgctgtc ggagttgaat
     1561 tttttcgcgc ccttacgcac cgaaccagaa gtcctcgccc ggcagcgcgc cgccgacggc
     1621 ggaagggttg agcgcgaaga gcgtttccag cgtgccggtg gccgcggaga tcatttcctc
     1681 gccggtcaga cacaccagat tgcagttcgg gatggctttc gccgcgatgg gcgccttgac
     1741 gatgttgagc gcgccgcaga tctcgccggc atccgcggga tcggcgttga cccaatcgac
     1801 gctctccgcc atctccgcga ggaacgcctc gaccgcctcg gggtgggctt cggcaaactc
     1861 ggcgcggaca acgacacccg ccgtcacgca gcggctgccg ttatcgagcg cgtcccacgc
     1921 ctcggaaagg ctcaccgcgg cgcggaagcc ctcgccgagc tgcgcggcag cggcggtgac
     1981 atagggctgc ggcagcatcg caatgccgcc gttttcggcc ttgaggagcg cgacgacctc
     2041 gctcggctcg ctcttccatt ccacggtcac gtcggtgtcg agatcgagcc cgttctcggc
     2101 gagcagatac cgcaggaaat attccggcgt gctgcccttg ccggtcgcgt agatcgtttt
     2161 tcccttgaga tcggcgagag actgcacggt ctcgccgttg aactccgtga tatacagcac
     2221 gcccagcacg cccacggcgc agagcttcac gccgccggag gtcttattat aaagtacgct
     2281 cgcaagattg agcggcaccg cggcgatgtc cacgccgccc tgcagaagct gcggcgtcag
     2341 ctcgtctgcc gcgccgtaga gcgcgtattc gtagtcgttc gccgccgtgc cggcgtcggc
     2401 gtccgaaaag atcttcgcca tgcccatggc cgtggggccg gtgagcgcgg caaggcgtac
     2461 cgtgacggtc tcggccgcct cttccgccgc cgcgaccggc gccgcggtcg gctcggccgg
     2521 ggcttccgtt gcctcaacgg gcgcgtccgt cgccgcgggc gcttccgcgg cgggcgcgcc
     2581 gcaggcggca agagcgaaaa ggagcgccag cgcaagggca agcgcggtaa gtttttggat
     2641 tttcatagat cgggttcctc cgcttttgac atttcgctga cagtatagta tagtataccc
     2701 gaattgtaag ttgctgcggc aacaaagcgg ggtgagacgc acgacagata cggaaagatc
     2761 ggcgcttttg cgctgcccgc tgttcgcgcg catcgccccc aacgacgttt ttgcgctgac
     2821 agaatgtctg ggcgcggccg agcggcggta tgagcgcggc gaggcgctct gcctcacggg
     2881 cgacgcggtg agcgcatgcg cggtggtcct ctcgggtggc gtgcgggcgg agagccttct
     2941 gccgtccggc gagcggagcc tcacggcggt gcacggcccc ggcgcgctcg tcggcgacgt
     3001 gctcatggca acgcgcgaca agaaaagccc cgtggacgta atcgccgcgc agaataccgc
     3061 ggcacttttc ctgccgtacc gggcgatgat ggcgtcgtgc gcgcacgcgt gcgagcacca
     3121 cgcgctgctg cgggaaaacc tgctcgcgga gatcgcggaa aagtactggc ggctgcgccg
     3181 ccgggcggaa tatctcgcgc ggcattcgct ccgggcacgg atcgcggcgt ttcttcttga
     3241 cgccgccgcg gacgccgggg gaaacacgtt ttcgctcggc atgcgccggg aggaccttgc
     3301 cgcctatctc ggcgcgaacc ggagcgccct ctgccgcgag ctttcgcggc tgcgcgccga
     3361 gggctggatc gactgctgcc gcgacagtgt gcggctcatc aataccgccg cgctcgcaaa
     3421 gagcgccgcg gcggagaatc ggagcggaga aaaatgaacg aaacagaaaa ccagaaagag
     3481 aactttcggc agcgctgcgc cgtcttacag gatgaaaacg cttcggaggg gtttacggtt
     3541 ccaatggaag aaacggcaac gaaaaaacgg ggaaacaaaa agacgcgcat agcgctcgtg
     3601 atcgcgctct cgctcgtgct gctcgcggtc atcgcgctcg gcggcgtgtc ggtgtattac
     3661 cgctcggcgt ttctgccggg cacggtcatc aacgggtacg actgtagcgg cgtgtccgaa
     3721 gcgggggcgg cggagtttct gctcggaacg gcaaagtcgc acacggcgca gctgcgcgac
     3781 gcgcagggcg atgccgtcgt cgcgctgccg ctcgagagct tcgtggacac cgacggcttc
     3841 acctccgcgc tggaagagta ttttgacgcg cagcatgccg aggccggtct cttcggctgg
     3901 atgacgaagg gcgagcggag tctggagacg ggcgtttaca ccgtttcgga caccgccgca
     3961 gcgagcgagc tgctctcccg tgtgctctat gacgaaacgc cccggcaggc gccggtggac
     4021 gcgcgcatcg ttctcggcga ggacggctac acggtcgatc ccggttcaaa gggcaatctc
     4081 gtaaatctcg caaactgcgt ttccgccatt gcggagcagc tgcccgcggt gcgcgatctg
     4141 cgcgaggaaa gccccgtgat cgaggcgaaa aacgccgtca tccggcagaa cgttacggcg
     4201 gagagcccgg agcttctcgc gcagcgcgcc gcgatcgacg cctatctcgc tacggaagtg
     4261 acgcttgatt ttcaggacgg gaacacctat acgctcacgc cgcaggatat ctggcgcatg
     4321 agcgatgtga cgctctcgga cgccgagggg cagacggtgt gcgccccggt gccggagaag
     4381 gtaaaggcgc tttcggacgc gctcgcggac gaatacgcgc tcgacggcgt ttacgccaaa
     4441 ttccacaacg ccgaaaagac ccgcccgtat atctactacc gcgtcggcga taccggctgg
     4501 atcctcgacc gtgacgcgct tgcctccgac atcgccgcgg cgctggagac cgaaaccgac
     4561 gcgaccgtta cgccgagcta cgatacatcg tggtactgga agcaggagta ctggttttat
     4621 aatttcacgg acaccttcgt ggagatcagt ctggataacc agtacatgtg gtactatgta
     4681 gacggcaagc tgctcgtgga aacgccggtc gtcaccggca acatcgccgc cggggacgat
     4741 acgcgccgcg gctgcttccg cattgcgagc atgacgacgg acacctacct cgtcggcccg
     4801 acgtggaacg accatgtgga atactggatg ccgtttgacg accagatcgg cctgcacgat
     4861 tcctcgtggc gcaccgagta cggcggggac atttacctga ccgacggctc gcacggctgc
     4921 gtcaacacgc cgctcgacgc cattgcgatg atctacaaca acatcaccgt cggcacgctc
     4981 gtggtcgtgt actgatgctt tatgctgtgc gttattgcaa aactggataa gcgctccaca
     5041 gaaaagctga cggccataca aaaggcggcc gcgcccgatg ccgatgggaa accgctgcac
     5101 gggcatatca cgctcgccgc gtatatgggc gaggacgagg ccgggttcat ggcgttctgc
     5161 gccgcactgc tgaaagacgt ttcacggttc gcggtgaaat atacgcatct cgcggtattg
     5221 gaagaaacct ccatcgtggc ggcgctcgcg gagaagaccg gcacgctcgc agcgctccac
     5281 cggcgcatcg cggaagtcta tggcggcagt ctgaacgaat ggaccggctc ggacgaagtg
     5341 tggctgccgc atacaacgct gctgtatgat ccgaaggcgg atctgcctgc cgtgtgccgc
     5401 aaaatggcgg cggactttgc gccgtttacc gccgggatcg tgggcataga gttttcgcgc
     5461 gtcacgccgc cgggctatga gatcctgggc cgcgccgacc tgcgcgattg acgaacggaa
     5521 taaagaaaaa caaacgccgg agcgcggcaa tgccgcgctc cggcgctttc tgcgcggaga
     5581 gaaaataatc tcgattacca gtgcgcccga tccgcccggc agcggatgac tgtacgcgtc
     5641 tgtacccgtc acgggttggt acgcagaaga gtgtgaatgt gtataaatcc accgttaatt
     5701 tttcttgata tcgtgtaaag aaaatgctaa agtatttttg cacaaggcag aaatactgcg
     5761 cgccgcgccg gcctgtgccg ccgcggcgtt tttatcccca aaggagagag tcgggatagc
     5821 catggagaaa aagaaaaggg tcagcttcgg tatgttcctt gtgacgatcg gcattgtgta
     5881 cggcgacatc ggcacatcgc cgctgtacgt gatgaaatcg atcctcaagg gcaacggcgg
     5941 catcgcgcac gtgaatgagg actttatcct cggtgcgctc tcgctcatca tctggacgat
     6001 cacgctgctc acgaccgtca agtatgtgct catcgccatg cgcgcggaca ataacggcga
     6061 gggcggcata ttctcgctgt acgcgctggt gaaaaaggta gcgccctggc tcatcttccc
     6121 ggcgatgatc ggcggcgcgg cgctccttgc cgacggcgtt ctcaccccgg cggtcacggt
     6181 gacgacggcg gtggagggtc tgcgcagcat cccggcgatg aaccgctttc tcggcagcgg
     6241 gcaggcgcac gtcgtcctta tcaccctcgt catcatcagc acgctcttcg ccgtgcagtg
     6301 ggcgggcacg agcaagatcg gcaaggcgtt cggcccggtg atgctcgtgt ggttctcgtt
     6361 cctcggcatc atgggtctgc tgcacgtttt cgaccgcccc ggcgtgctgc tcgcgttcaa
     6421 cccggtgtac gcggcgcgca ttttggtttc gccctataac aaggcgggct ttatgatcct
     6481 cggcagcgtg ttcctcgcca cgaccggcgc ggaggcgctc tattccgata tgggccacgt
     6541 cggccgcggg aacatttatg cgagctggcc gtttgtgaag gcgtgcctga tcctgaacta
     6601 tctcggccag ggcgcgtgga tactgacgca cacggcttcc ggcacgctcg ccgccattcc
     6661 cgacatgaac ccgttcttcc agatgctgcc ggaggcgctg cgcgcccccg ccgtcgcgct
     6721 cggcgcgctc gcggcgatca tcgcatcgca ggcgctcatc acgggctcgt acacgctcgt
     6781 gtccgaggcc atccggctcg atctcatgcc gcaccttgag gtgcgctacc cctcggacac
     6841 gaaggggcag atctacatcg gcgcggtcaa tacggtgctg tatctcggct gcgcggcggt
     6901 agtgctctac ttccggacga gcgcgcgcat ggaggcggct tacggtcttg ccatcaccgt
     6961 gacgatgctt atgacgacgc tccttctcgc ggtgtatctc tggagcatcc agaaaaagca
     7021 ggcactcgcg gtcggcatcg cgctcgtgtt cggcgcgatc gaggcggtgt tcttcctctc
     7081 gtcgctgagc aaattcgccc tcggcgggta tgtcgcggtg ctcatggcgc tcgtgctctt
     7141 tctcatcatg gtcgtatggc ggcgcggcac gcagctcgag caggagtacg ccacgcgcct
     7201 gccggtcggc gattatctcc cgaatctcga tacgctccat aaggatactt cgctcccgca
     7261 gctcgcggac aacctcgtct ttctcgataa ggccggggac atggatacca tcgaccgcga
     7321 cattctctac tccatcctcg acaaggaccc caagcgggcc gcggcgtact ggttcatcag
     7381 catgaacgtt accgacgagc cggacacgag gcgctacagc gtcgagacct atggcacgga
     7441 ctacatcttc cgcgtccggc tcgatctcgg gttcaagtgc caccagcgcg tgaacgtgta
     7501 cctccggcag atcgtgcgcg atcttatcga gagcggcgag ctgccgccgc aggagcggaa
     7561 atactcgatc tacggcaagt cggacgtagg ctcttttaaa ttcgttttcc tgcacaaggc
     7621 cgtgccgtcc aagtcggagc tttccttcct tgacgagctg gtgctcaaca cgaagtatgc
     7681 catccggcgc gttgccggct ccaaggtgcg ctggtacggg ctggacacgt cctcgctcgt
     7741 cgtggagacc gtgccgttca tcgtcggcgg caaaacgccg cgcagcagcc ggctggaaag
     7801 agtaaaataa aaaaaagaaa ccgcccccgc ggctcaaccg agccgcgggg gcgggacttt
     7861 ttccgacatg ttgtccggcg tttccccgta tcgctccggc gcgcacttga cgcatgtatt
     7921 ttaccctgtt tcgcgcgggc acagaggcgt tggatattcg gagggcggag caagtgttcg
     7981 cagctgcgca gcagacgatg gaggatatgt acctatccgc gaagaacatc aaaatgtaaa
     8041 acgtgtcaat ggggaatcct ggctgacggc atttgcggca gagagagttc cccgttggca
     8101 acttttagcg tatttggaca atccatgcaa agaaaagcca accccgcggc cttaaaaaag
     8161 gtgacggggt tggctttctt acggcatttc gtccggtgta tacccaagtc ggagggagat
     8221 ctcccgcgcg gtacgcttca gctcagggag gagcacggtt tcgatgcgcc ggtcggtgac
     8281 gcgctcaatg ttgtcaaacg cgctgatcgc cccggccacg ttgccggaat agtcgtagat
     8341 cggtacggcg atgcaccggt ggccgatctc acattcctcg tcatccacgg cgtagccgcg
     8401 cttgcggatg agtgcaagct ctccaaagag cttttccggg gtggtaatcg tattctccgt
     8461 cagccgttca agcccctttt cggcaataat ctcctcaacc ttgcggtatg aaaacgagct
     8521 gagcagcact ttgccggagg ctgtcgtgtg gatgggggcg tttttcccga tgcggagcat
     8581 cgttttcatc atccagcggg gattatccac aaaatccaga tacaaaacgc ctcgatcctg
     8641 ctcaatggcg aggagcgttc cggcgttcat gacgttggcc gcgtggttca aaaacggcga
     8701 ggcaatgctg cgcagcgaaa gattggattt taccgaatcg gccagacggc agatcttcca
     8761 tgtgagcgcg taacagccgg tcacatcatc atggtaggcg tagccctgcg cgcacagtgt
     8821 cttgagatag cgcagcagcg tcggctgcgg catatcgagc ttgtcagcga gatccgtcag
     8881 ccggttcggc aaccggtttg ccgccagaca ttcgatgatt cggaacaatt tgtctgagga
     8941 aagattgcct ccttcttttc cagccatcac tttccctccg ttctgtcgat tttcgttgtt
     9001 tttatattat acgatttcca caaaattttc aagagatttt tggaaccgtt tatccttttt
     9061 aaaatcctat ttcaaaaaat gaaattaaag tacgcggcaa aatccgctat gaaaaaaaca
     9121 gagtttcaaa aaacagaaca attgcacttt agcacaaaaa atcgctacag agcggtatgt
     9181 tttttggact ggtaatttgc ctgtactgct gtataattaa cgaaaaaatt ccggcggtat
     9241 gaatggttta ttctccgctg agatagaaaa ggagcgggaa acggatggac atgttgtttc
     9301 tcagaccctc cacgcaggag gaggctttgg cgctgctgac acagtataag caggatgcca
     9361 tgatcgttgc cggcggtacg gacgccgtgc tgcggctgac acagaaaaag accgctcctg
     9421 ccgtcatcct ctcgctggac gctattccgg aacggcacga gatttccgcc tctgacgata
     9481 cggtgcgtct ggcgtccggc gtcacctata acgagctgct tgcaagcgat gtcctccggt
     9541 tgtaccgggg gctgcgcgag gcggtgctgc atctggcgag ccccgccatc cgcgcggtgg
     9601 cgacgccggt ggggaatatc tgcacggccg ctcctgcggc ggattgtaca actatgctct
     9661 tcgcgctcgg cgcgagcgcc gttcttgcgt caaaccgcgg cgagaggcgc gttccgctgg
     9721 atgcgttttt cgtttcgacc tacaagacca tacgtgagag cgacgagctt gtcacggcca
     9781 ttgagatccc ggcgctccgg ccgggagagg ggacaggcta ctgccggctt tcccggcgca
     9841 aggcgcagga tatcggcaaa attctcatcg gttgccgcgt gcggctcgac ggggaacgta
     9901 ttgcggaagt ctccctgagc ctcggcgcgc tcaaccccac ggtcgtccac gctgcggaac
     9961 tggaggggct tctcgtcggt aaaacaccgg aggaggcgct tgctcttgcc gcagtgacgt
    10021 atcccgcgga ggcaaagctt cgcgagagct attacaaaac ctacaagcag gacgccgtct
    10081 gcccggcggt cgtccgggcg ctttcgatgg cgctggccga tgcgaaagga gagagagcat
    10141 gaaagtttca tttgttctca acggaagaag tgtttcctgc gaggcggagg ccgacgaggt
    10201 gctcgttgac acgctgcgca ataccttctg tctgaccggc acaaaaaagg catgcggtac
    10261 gggcgactgc ggtgcgtgca ccgtactgct cgacggcgtg gccattcgct cgtgcattta
    10321 tctcaccgcc atggctgagg gacatgagat cacaacgatc gagggcgtgg gcacgctttc
    10381 cgctccccat ccggtgcagc aggcgttcgt ggatgcaggc gcggtgcagt gcggctactg
    10441 cacacccggc atggtgctca cgacgatcgc gctgctaaat gttaatccaa acccgagcga
    10501 ggaggagatc cgcgtggcgc tttccggcaa tctctgccgg tgcaccgggt atgaaaagat
    10561 cgtagaggcc gtccggctct cggcaaaacg cctgcgcgtc gaaaagaaat aagaaaagag
    10621 acaaaagcaa caaatccaag aagggggaca acaacaatgg aagtcaaaca ctatgaggga
    10681 aacggccggc tgagcgttgc tacggagtgc aacggcatca ttcacatgac aggccgtacc
    10741 tgcatgccgg gctattaccc ggacattccc agcggcgatg acgtgaagtc gcagaccgcc
    10801 ggtgtgctga agatcattga caacgtgctt gccaagtatg gctcggataa ggagcacatt
    10861 ctatttgccc aggtgttcct caaggatgtt atccgcgatt ttgacgatat gaacgaggtc
    10921 tgggagagct gggttacgcc cggctgtgaa cctgcccgcg ccannnnnnn nnngtgcagg
    10981 ccaagatggc aaaggaagaa gcgcttgtgg agatcgtcgt gaccgccgtt cgcaaggacg
    11041 cttgacggga ggcgcttcca tgcacgaatt acactatgtc ggccggcgca tgcagcgccc
    11101 cgacgcactg gcgaaagcga cgggggcggc acagtatacc gcggatctcg tcgttcaccg
    11161 gcgggatgtg ctccatgcca aggcactcta cccgccctat ggccacgcga agatccttcg
    11221 catcgataca tccaaagctg cggcgctgcc gggcgttgcg atcgtgatga ccgcagacga
    11281 tcttcccggc gagaacggct acggcggcat catccacgat aagcccgtca ttgctaagga
    11341 cgaggtgttt tatgagggcg accccgtcgc tctcgtggcg gcggaagatc tcaagaccgc
    11401 cgagaaggcc gtgtcgctca tcgaggtcga atatgaaccg ctggagagct atgacagccc
    11461 cgccgatatg ctgcgcgagg acgcgccggt gctccaccga gagtacccca tcccgcgcaa
    11521 gggaaacatt tccgacgagg tgctcatccg caagggcgac gtgaaaacgg cgttcgagga
    11581 cgcggacgtc gttgtggaca actggtataa gaccccgatg ctcgatcacg cctatctcga
    11641 gccggactgc gcgctcgccg agcccgaccc ggtgcagggc ggtatcacga tctacagccc
    11701 gcagcacgcg gtccagctgg cgaaaaaggc gctgtgcgga gctttcaaca tgccgcagag
    11761 caagatccgc gttatcagtc aggttgtcgg cggcggtttc ggcggcaagg aggactccac
    11821 gttcgacgca agcgtcgtcg cgggcgtgct cgcgatcaaa acggggcggc cggtatttta
    11881 cgagctgacg cgcgacgagg tatttaaaaa caccggtaag cgtcatgctg ccgccatcca
    11941 tcaccggctg gctgcggata aggaaggaaa catcctcggc attgaggtcg attccattat
    12001 cgataagggc gcgtggaagt ccattgatgc catccccgcg cgcacggcac agtacgccgg
    12061 cagcgtttac cgcatcgata atgcgctcac gcattcgtgg tcggtgttca cagaccatcc
    12121 ctacggctgc gcgttccgcg ggctcggctg cccgcaggcg acgttcgctg tagagtcgca
    12181 gatggatgaa ctggccgaga aactcggtat cgatccgatc gacctgcgca tgaaaaacct
    12241 cctgcgcccg ggcgatacga cgatctgggg acaggaaatg ctcgaggagc gcgggttcgg
    12301 cattgccgaa tgccttaccc gcgtccgcga ggcgattggc tgggacaagc ctctcacgcc
    12361 gagtgaagat cccgacgtcc gccgcggcaa aggcgtggcc tgctatatgt acggcaccgg
    12421 taccggttat atcaccgacg gcgcgcactg ctttgtgcag gcgcagccgg acggctcgct
    12481 gaatatcggt atttcctcca atgagctcgg ccagggcttc ctcatcgcca tgcgccagat
    12541 cgctgcggac acgttcggcg ttccgatcga aaaggtctat ctcgactttt ccgattccgc
    12601 cgcctcggtg gaagcgggcg cgacggtcgc ttcaagaacg accgtgttcc tcggcaatgc
    12661 cgtgatgaac ggctgcaatg tcctgcgcga acggtttctg ccggtagcgg cgcagatgct
    12721 cggcgcgaag aaggaagacc tcgacgtgga taacggcttc gtattcgaca cgaccgacga
    12781 ggcaaagcgc gtgccgtatg cttccgtgat ttccaaggcg tttgccatgc agatcccact
    12841 cgcgtgtgtc ggttcgtggt atccgccgaa agcctatgcc gacgaaaacg gccagggcgt
    12901 gaaaatgcac acctatgcct ttgccgcgca ggccgcgatc gtctccgtaa acgtcaagac
    12961 cggcgaggta gcggtggacg acacggtgct cgccgtggac gtcggtcacg ccatcaaccc
    13021 ggatacggtg gagggacaga tgcacggcgg catggcgcag gccatcggct ggtcgctcat
    13081 ggaagaggaa ttcatgaaag acggaaaaat gcgcaaccat acgttccacg actatctcat
    13141 ccccacggcg atggatctgc cgaagctgcg cacgatcatc gttgagcatc ccaactccct
    13201 cgggccttac ggcgcgaagg gaatcggcga gccgcccatt gtcggcgcgg cgcccgccat
    13261 tcacaatgcc attcgcaacg ccgtcggcgt gagcattaac gagctgccca tgacgccggt
    13321 gcgtgtgttg gacgcgctgc gcaaagcgcg cgcggagcaa taaaaaagag agaaaaaaac
    13381 aaggaggaaa aatcaatgga cggtatgtta gccctgacga tcgtgtgtgt tgtattcgcg
    13441 atcggcgact tcatttccga caaaacgcac gccatcatcc ccgtactgct cttcgcctcg
    13501 gtcacgttcc tgatcctgtt ctggacgggc gttgttccga cgactctgtt caccgatgca
    13561 ggccttatcg ccatttccaa tatgatgacc ggcatcttca tcgtcaatat gggaacgctt
    13621 cttgatttca aggagttcgt ttcccagtgg aagaccgtgg tgcttggcct cgcgatcgtt
    13681 atcgtgggcg gcattctgat gttctttgcc tcccgcccgg ttctgggcaa caacttcgcc
    13741 gtggctctcg tcggcccgat caccggcggc tgggtttccg ccgtcgtcgt caacgacgcc
    13801 atcaaggcgc tcggctctcc cgattccctc aagctcgccg gtgttctggc cacgatcatt
    13861 gtcgcggtgc agggcatcat cggctaccct ctcgcctctg cgttcctgcg cagcgaggcc
    13921 aagcgcctcg tcaagggcta caacgccggt accgtgaagt ggacgcgcac cgtctttgaa
    13981 ggcgacgagg ctgcgcagac cgccgttgtc aagaaaaagc tcattccccc tctggcggcc
    14041 aagtaccaga ccccgttcat cctgctcggc aaggcctgcc ttgtctccct gctctcgttc
    14101 tggatctcca atctcaccgg cggcgccgtg cacagatatg ttgtctgcct catcatgggc
    14161 gtcgtcgcgc aggagatcgg cttcctggag cacgacgtta tgaagaagtc caactgctac
    14221 ggcatcggcc ttgtgatctg catggtctcc atcctcgtgt tcctcaccgc cgtcacgccg
    14281 caggatgttc tcagccttct ccccgtcgtg cttggcgctt tcgtcatcgg ccttctcagc
    14341 atgttcgtga tcgccgttgt cgtcggcaag atcttcaagt tcagctggca gatggcctgc
    14401 ggcatcgcct gctcctgcct gtttggcttc ccgggaacgt ttgtcgtctc cagcgaagtc
    14461 tccaagcaga tcggcacgac cgacgacgag cgccagatgc tgctcgacaa catcatgccc
    14521 aagatgctcg tggcaggctt tgtctgcctg acgatcacct cggtggtcat caccggcgtt
    14581 ttcgtcaact acctcgtggt ataaataagc attacattca tgacccgccg tttccccggc
    14641 gggtcataca aacgaaaagg agtatacacc cgatatgagt gaagctattc gcaagcagta
    14701 ctgcgtcggc ggcgtctgga aggactccaa aacggagaaa tggatgccgg tgaccgactc
    14761 gtccacgggc gaagtcatcg cggaagtccc ctcctgcacg caggacgagg tgctcgaagc
    14821 gatcgacgcg gcggacaagg cgtttaagga gtggagtcag gtctccgttt ccaagcgcac
    14881 gcagatgatg ttcaagtggc gcaacattct cgttgaacac ctggacgagc tgacgtatct
    14941 ttgcgccaag gagctgggca aaaacctgag cgaggcgcgc ggcgacgtgc tcaaggccat
    15001 cgagccgacg gagcttgcct gtgctacgcc gtttgtttcg cagggctcgg cctcactgca
    15061 ggtcacgacc ggatttgata cggccaccta ccgtatgccg cttggcgtcg tggcgggcat
    15121 cgtgccgttc aacttcccgg cgatgatccc ctggggctgg atggtgccgc tcgcgatcgc
    15181 cacgggcaac acggtcgtgc ttaaggcagc ctcgcagacg ccgctcaccg ccatgcgtat
    15241 catggaactc ttctatgaag agggcggctt ccccgcgggc gttgtgaacc ttgtcacgtg
    15301 ctcgcgcggc gaatccgagg tactgctgac cgacccgcgc gtcaaggcca tcacgttcgt
    15361 cggcacgacc gaggtcggca agcacatcta ttcgattgcc gcggcgcacg gaaagcgcgt
    15421 gcaggcgcag tgcgaggcga aaaaccacgc gcttgttctc gaggactgcg atctggagag
    15481 caccgtaaac gcgatcatta actccaccta tggctgcgcc ggcatgcgct gcatggcgct
    15541 gcctgtcatc gtggtgcagg aaaacatcgc ggacaaattc gttgccctgc tcagggaaaa
    15601 ggcaatggcg atgaaggtcg gctgcgccta tgaccccgag acgaagctcg gcccggtcgt
    15661 gagcgccaag cacaagaagt ccatttgcga ctggattcag cgcggcatcg acgacggagc
    15721 ggagctcgtg ctcgacgggc gcgatatcgt agtgccgggc tatgagaacg gcttcttcgt
    15781 cggcccgacg atccttgacc atgtgacgcc cgatatggcc atcggccagc gcgagatctt
    15841 tgggccggtc acgcttatca agcgtgtcaa gacgtttgaa gaggggctgg ccgtcatgaa
    15901 cgccaacccg ttcgccaacg gctcggccat ctttacgcag agcggctact atgcccgcca
    15961 gtttgaaatg ctcaccgacg gcggcatggt cggcatcaac gtcggcatcc ccgtgccctc
    16021 ggcgtatttc ccgttctccg gcaacaagga ctcgttcttc ggcgaccagc atgtgcttgg
    16081 cctggacggc gtgcgcttct atacccgcgc caagaccgtc accaagcact ggtacgatga
    16141 acactcccgc aaaaagaccg ttgacacctg ggaaggcacg gtcgagagaa actgaacacc
    16201 gcgaaaaagc tcccgatgca gctgcatcgg gagctttttg cacttgccaa agcgaggctt
    16261 ctttgctgtg tcagttctga taaaagacat cctcctccgt gcggggcgcg aagcgtgcga
    16321 gcgccgcggc gtcaagattg cagcggtgca tgtccacggc gctgatacgg cggttttcgt
    16381 aggtcgtata gtagtaaatg ccgcgctgcg cgctgcagca ggaggtatag atcgtgatct
    16441 cgtgcttgcc cggttctacc tcacagcagc cgcgtacctg ctccaccgtg ccgagaatgt
    16501 ggaaaaactg gctgacactc tccgtttccg aagagccgga aacggcattc gcgcgcgtaa
    16561 aggccgcgcg cacgaagcgc gagctcgacg agaggtcgcc cggcagcccg agcgcgccca
    16621 taccgcggct gtaggagcgc agatcgagcg cgggcgcgaa cgtattttcc ggctgccgcg
    16681 gggagagcga tcgatagtcg ttcagccgga agagctgctg cgggaacggc gggttgttcg
    16741 tcaggacccc ggcggggtta tcatagacgt gcagaccgtc cgcggtggac tcgatcgtga
    16801 cgcattcgtc cttatccgca agcagccagt ggagctgcgc ggcgggaagg gcggcggaaa
    16861 acggcgtgcc ggtgagattc gtcttcccga gcagtacgcg cgcctcggaa acggaggcgc
    16921 actgcgcgag cacccacggg atgaattcaa actgcgcgac gtcgccctcg ccgccgcgcg
    16981 gcgcggcgta ggcggcgttg ccgacaaagt tcagccccgc catgcagaga cctttttcgt
    17041 tcatcgcgtc gtagtagagc ggatagccgt ccgcgacgtg cgccatgccg agcagggcga
    17101 agtgctccga aagcgccttg ccgccgctaa aggcgagcgg gaagcgccgg ggcgtcacga
    17161 cgatctcgtc gccgtaggaa aactcatagt caagcgtccg gccaaaatag aatccgttgg
    17221 ttttatatgt cgcggctgtg cacatgatgc aatacctcgt ttcctttcgt attgtctccc
    17281 tttacgcaag ggggccttgg acaaaagacg gaggagctct ccctctatct tatagtatac
    17341 ctcaatcgag cgctttataa tagggacaga tatcggcata tgtcccgtcg tcgagatagt
    17401 ttcttacggc agtggtcaag gggagcgctc cttttaaaaa atatcaggca ttttccgctc
    17461 cggcagtgag tctgccgaaa cgcgtgaaga acatcgcggc ggtcgtgcac acggcgagcg
    17521 tgtccgcgac gggctcggcg agaaacacgg cgaaggcggc gtttttaaaa atgccgagcc
    17581 gcggcaggag caggatgagc gggatgagca ggatcacctt gcgcagcagc gcgaggaaga
    17641 tcgccacctt tgcctgcccg agcgcaacaa agctctgctg gcacgcgatc tggatgccga
    17701 aaatgccgct cgcggccata taaatgcgca tggcccatgc ggtgtaggag gaaagctccg
    17761 cgtccggcgt gaacattccg gcaacggcgc cggggaagag catgcacacc gcccacacga
    17821 gcatggagcc tgtgagacag cttatgaaca ggatgcggaa cgtttcgcgc acacgctgtc
    17881 cgttccccgc gccgtagttg aagctcataa tgggctgcgc gccctgcgtg agaccgtgca
    17941 gaagctgcat gcagagactc atcgtggtcg taaggaccgt caccgcgccg acggcggtat
    18001 ctccggcgaa gcggaggagc gtcacattga agctcaccgc gacgaggttt tccgtgatct
    18061 gcatgaggaa cggcgagagc ccgagcgcga gacaggggag aaggagcttc atgtccggca
    18121 gaaggttctt cgcgcgcagc cgcagcgtag atttttccga gcagaggaaa cgcacgaccc
    18181 acgcgcacga aacggcctgc gagaggatcg ttgcgagcgc tgcgccgcgc acgcccatgc
    18241 cgaacacgaa catgaacagc gggtcgagca cgatgttgag cgccgcgccg atcgtcacgg
    18301 tgagcatgct cgttgtggaa aagccctgcg cggtgatgaa ggcgttgagc ccgagcgaga
    18361 gctgcacgaa gatcgtgccg agactgtaga tcgagatata ttcgagcgcg taggggagcg
    18421 ttgcgtcgct cgcgccgaag agacggagga tcggcacggc gaaaagctgc atgatgagcg
    18481 tgagcgcgag cgagatcgcg cagagcgtga aggtacacgt gccgatgatg cgctccgcac
    18541 tgtccggggc tccctttccc atgagcatgg aaacgcgcgg cgcgccgccc gcgctcacga
    18601 gcgcggcgaa tgccgagatg atcatgatcg ccggcatcgt cacgccgacg ccggtgagcg
    18661 ccgccgcgcc gtagaccggg atgtggccga tgtagatgcg gtctacaata ttatatagaa
    18721 cgttcacgag ctgcgccgcg atcgccggaa gcgccagctt ccagaagagc ttgccgaccg
    18781 gagcggtgcc gagcgcggga tctctgcctg tttttacgtt catgtcaaac ttccttttct
    18841 gtcaaaagtc aaaagctccc gggatcggga gccgttgacg cttattcacc cgtatgatcg
    18901 agaaatgcgg gaatgtccat ggcgtgttca aacacgcgct cgcccagcag acccacggtg
    18961 cgccacgagt cgcggtagcc gttctttttc cggtcgggga tgtacacgaa ctccttcccg
    19021 gtcttggccg gatcgtttgt aaagcagatc ttccggtacg gcgcggcgag atactccgcc
    19081 agcccgccgt ttttggaaag gcttgtttcg tccgccatga gcaggatctc atatccctgc
    19141 gcgcggcagg cgttgaagcg ctgcagacgg cgctcccagg cggcgagaaa ttcctcgcgg
    19201 cttttgtagt gcgtgccctc gatgactgcc tcgccgagtg tgaacatcgg gtagctccgc
    19261 tccggcgcgt ccatggggac gggcgtcatg gcaagatatt cgtccagatg cgtgcacagc
    19321 ttttcaaacc ggtggaacgc catattgact gtgggcatgg taaactgcat cccaagatcg
    19381 tgcagcagca gtccgccgtt gcagtcgctt gcaataagga gaaaccggcg gtttttcact
    19441 ctccggcgca gccagagcag atacggccac cgccgccacg accaaaccgt gcgcagaatg
    19501 cttttgagct tctctttcat cgtatccccc aacgttttct taccgcttac cataccacag
    19561 ccgcagcgca aatgcaatca gggcgcactt gcaaagagcc gtgcggcgcg gtaaaatgaa
    19621 cgcgataata ctttttccgg gaggaacgga atatggacct cagcaaactg cagaacggca
    19681 gcgacgtgcg cggtgtggcg cttgctggcg tggagaatga accggtaacg cttgacgatg
    19741 cggcggtgcg cgccattgcg ggcggctttg cgcgctttct gcgcgcgcgg ctcggaaaag
    19801 agagctgccg cgtgagcgtc gggcgcgact cgcgtctttc gggcggggcg atcgccgaaa
    19861 cgctttgcga aacgcttgcc gcgtgcggcg cggaagtgac cgatctcggt ctctgcacta
    19921 ccccggcgat gttcatgacg accgtgacgg gagaccgccc ctttgacgcc gccgttatga
    19981 tcacggcgag ccatctgccg tggaaccgca acggactcaa attcttcacc gccgcgggcg
    20041 gcgcggagag cgcggacgtg cgcgagatcc tccgcctcgc cgcggaggag acgccgctcg
    20101 cccccggcgg gagcgtggcg cggggcgaat ttatggagat ctacgccgcc attctgcgcg
    20161 aaaagatcat cgccgcggcg aacgggaacg agaagcctct cgcaggcaag catatcgttg
    20221 tggacgccgg aaacggcgcg ggcggctttt tcgccgagcg cgtgctcgcc cctctcggcg
    20281 cggacacggc cggcagccgc tatctcgacc ccgacgggcg cttccccaac catgtgccga
    20341 accccgagct gcccgaggcg atggacagca tccgcgaggc ggtgctcgag agccatgccg
    20401 atttcggtgt tattttcgat accgatgtgg accgcgccgg ggcggtgctc tcgaacggcg
    20461 aggagctcaa ccgcaaccgc ctcatcgcgc tcatctccgc catactgctg cgtgagcacc
    20521 ccggcacggc gatcgtgacc gattccatca cgtccacggg gcttgcggag tttatcgccg
    20581 cgcacggcgg cgtgcaccgc cgcttccgcc gcggctaccg caacgtcatc aacgaggcgc
    20641 tgcggctcaa cgccgcgggg caggactgcc agctcgccat cgagacctcc ggccacgccg
    20701 cgctcaagga gaattatttc cttgacgacg gcgcgtatct
//
LOCUS       DAWAAE010000019        20421 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1163_length_20421_cov_3.681479, whole genome shotgun sequence.
ACCESSION   DAWAAE010000019 DAWAAE010000000
VERSION     DAWAAE010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 20421)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20421)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20421
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_20421_cov_3.681479"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4288..4297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18263..18272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4247 a         5988 c         6081 g         4085 t
ORIGIN      
        1 atctcgccca cacgctcaac aacaccgtcg tcgcaccccc gcgtatgctc atcgcgttcc
       61 tcgagaacca cctgcacgcc gacggcagcg tcgatattcc cgaggtcctt cgtccgtaca
      121 tgggcggcaa ggatcacctc gaaaagaagt aaaatgtaaa aatccagcgg cagaacgcaa
      181 tgagaaatgg cgttcacatc cgcgttctgt gccgccgtgc tcttcgggca cggcccggag
      241 aaagtccggc gcacgatcga attttgaggt gagaaagtga acgaaaagct gaaggctctc
      301 tatgaaaaat acaaggacct tatcccctac gtgatcttcg gcgtgctgac gacgatcgta
      361 aactatgtct cctactggct gttcgcgcac cctctcggct gcggtacggt attcagcacc
      421 gccgtcgcgt gggttttgag cgtgctgttc gcgtatgtga cgaatcgccg ctgggtgttc
      481 cacagcgagg cgaagggcgc caaggcgatc ggaaaagaat tcgccgcgtt tctcggcgcg
      541 cgcctcggca cggggcttct ggatatgctc atcatgtacc tgtgcgttga tcttctcggc
      601 tggaacgaca tggtgatgaa gctcgcgtcg aacgtcatcg tcgttatttt gaactacatt
      661 ttgagcaagt tcttcatctt taagaaaaaa gagaaataag acgcaaagcg aaaagccccg
      721 ccgggcagga aacctcctga ccggcggggc ttttgtcatg cgaaagatta tttgagcatc
      781 cgccagagcg tgctgcggct gatgtcgaga tgctgggcgg tcttcgtctg gttctgccct
      841 tccagctcgt agacgcgcag cacgatatcc cgcgtgatct cggcgagcgg gcggttgagg
      901 tcaataccct ccgccgcggc ggagggggtc gtcgtctcac gctcgagcac cgcacggacc
      961 tgcgcttcgg aaatatacga gctgtcagtg ttcactacaa gttcctgcac gcatttgacg
     1021 agctggtcaa tgttgtgcgg ccacgggaaa ttctgcaaaa ggagcatggc ctcgcgcgtg
     1081 agtccgatga cacgcgtgcc aaagcggacg ttcgtggcgt ttatgtagag cgtgacgagc
     1141 gtcgggatat cgtcgcggcg gcgcgagagc ggcggaagcc ggagtcggag cgcgtggacc
     1201 gtctccgaga gataggcgta gatcgggtca cgggaatact gccccgtgtc accggcgtaa
     1261 gatatgagaa accggttggc ccccagcgtg tgcgagcctt caagatagtc gagcagccgc
     1321 ggcgcaaaat tgcggtttac ggcgtcgaga tggcggagat agatcgtaga gccggtatcg
     1381 gcgagcggcg agcggtcatc ctcgagcaga agctcccaat ccctttccgt gagccgaccg
     1441 atatccacga ggacgagcgg actgtggcgc agacggctgt ggctgtggat gtactgcgcg
     1501 aagcggtcct tgccggtgcc gtaatcgccc tcgatgagta caggcgcgtc gagcgcggcg
     1561 tattgttcgc atacggcgcg cagccgctcc gcagcggcgt tgctgccgag atagtattcg
     1621 agcgggttgc tgctgagcgc gctgtccgcg ccggaggaaa ggtcacagta gcggatcgcc
     1681 tgccgcccgc cgaggagcga agcagcgcgg gagatggaga aaacggcgta ttcctcaccg
     1741 ccggaggcga gaagccgccc gcgcacggta aacatctcgt ttccgagccg gcggctgagc
     1801 ttcacgctgc tgcgcgcgag gacatgcgaa acatgctggc gcatcgcgtc ggcaagctcc
     1861 ggcggcatgg gatcgagcgt tgtgaagacc tctcggacgt cgggtgaata gacggcgaag
     1921 tcccgctcgt ccgaaacgag cagatcccgc agcagagagg cgtgccggtc gagcatctga
     1981 taccggagac agatatcctt cgcggtgttg atcgccgact cgatgctctc catgccggag
     2041 acgatcagca gcccgtgcat gccgtactcc tgcgcgcagg ttgtggagat catatcgccg
     2101 acgatgatgc gcgtcccctt atcgcgcagc gccgagagct tttcgcggca ctcctgcggg
     2161 ctgcggatcg tgaccgtagg gatggaaaat tgcatgatct cacagagctt ttccgccgga
     2221 tgcgtgatgc tcgggtagcc gacgatcgcg aaatcatcgt ccatgccctg cgcgagacgg
     2281 atcgtgcgca gaatatcgta ggcggagggg acgatatcgc agatggggat atccatcatg
     2341 tgtccgcgga gcacgtcggc cgtgccgccg cgggagatca ccgcatcgat gccgcggcgg
     2401 ttgtcgagcg ctgttttcag tccgtcgtcc agattgccgc tctggatgac gagctccaca
     2461 tcgtcgcgct gcgcggcgat gttcgtcatg agatgataca ttccttcgta gggggcgatg
     2521 cagagaaagc gcggcttttt catttctagg ccctccgtgt gtcactgttg catggacgca
     2581 attctaaaat aaaacaaaaa ggatcataac gcaagtgatt tatttttaaa cagccattta
     2641 acacggccaa acctgaacaa cgtctcggtt taattttgtg cataaagctc aaaaaacaga
     2701 gggagaattt agcggctggc ggcagaatga aacgacggag aaaaatattg ttcaaaaatg
     2761 aaacacaaaa gggcgctcta cggctttcaa gcggacggga tgttcctata taatttgggc
     2821 ataaaagcca gccggggaca gtatacccca aaaaagagaa aggaagcgca ccgcaaacga
     2881 tgataaaagt cctggttctt gccgatgacg tgaccggcgc gctggacgcc ggtgtgaaat
     2941 tttcggacgc aggtctgaaa acgaaggtgt ccctcctccc gaagatcgaa gaaacggata
     3001 ccggcgcgag tgtgctcgtg ctgtgtgtcc cgacgagaca cgaaacgccg gagacggcat
     3061 accggcaggt ccgcgctctt tcggcgcgtg ccgcggaaaa gcgcatcggc tgcatcttca
     3121 aaaagaccga ctccgcgctg cgcggcaacg tcggcgcaga gctcgccgcg gtgctcgacg
     3181 gcagcggtga gcatgagctc ttcttcatcc cggcgctgcc ggcgatgaac cgcgtcacga
     3241 taaacggcat ccattatatc aacggtgtcc cggtacatga aagtgtgttc ggcgacgatc
     3301 cctttgagcc ggtcacggaa agctacatcc ctgcgctgct ggaaaagcag atgaacgagc
     3361 ctgtcgaggt catcaccggc gcgactcctc ccgcgccggt gaactcgggc aaaaggatcg
     3421 cggtgctcga ctgcgagacg caatccggca tggagaagct ggcccggacg ctgtacgagt
     3481 ccggtcggct tcacgccgtg gccgggtgca gcgggctggc ggaagcactg gccgcggctc
     3541 tctgcggcag tgtcggcgag accgaagcgg agccggagac cgagcggctc accgttgtat
     3601 gcgggagcat gaacccgatc tcgctcgaac aggtggctta cgcggaaaag acgggctatg
     3661 ctgccgtcag cgtgccggtg gagcggctgc ttgagacagg actcgaaagc gccgagggca
     3721 aggcatttct cgatgcgctc tgggcggaat acagcaaaaa tgagcatctc ctcatcgata
     3781 cgggcgctgc caacgccgcg cgcggcgagc tgcccgagga gatcggaaag agcgttgcat
     3841 cgctcctcgg cgcgatactc aaggacctcc ttgaccgggg cgccgtctcg cggcttatga
     3901 tcatcggcgg cgacacgctg cttggtttta tggacgccat cggctgccgc gagctctccc
     3961 cccagcggga aatcctgcag ggcgtcgttg aatcggaata tatctaccgc ggcgtccggc
     4021 actcggtgct ttccaagtcc ggcggtttcg ggcagacgga tcttcttctc cgactcgaaa
     4081 gcgaagcgcc ggtccatgca taataaacga aaggaacgga tgaaaaatgg ataagtacaa
     4141 tatggttctg ccgcgcatca cctacagcgg cgtgaatgcg ctggaaaatc tccgccaggt
     4201 cgtcgacggc tgtaaaaagg ccgcgatctt caccgacaag ggaattctcg gcgcgggtct
     4261 tgtggatctg ccgcaggaga tcgtccgnnn nnnnnnnccg tgatcctctc cgacatacct
     4321 gccgagccga gctacggcgc ggtgcagacg ctggtcgacg agttccgcac gctgggcgcg
     4381 gatatcatca tcgccgtcgg cggcggcagc gcgatggaca ccgcgaagct cgcgagcatc
     4441 ctcgtaaccg acgaatatac ggtgaaggat cttctggacg atcctctccg cggcaaaaag
     4501 accgtgacga cctgcttcat cccgacgact gccgggacgg gctccgaagc gacgccgaac
     4561 gccatcgttg ctgtgccgga aaaggagctg aagatcggta tcgtgaaccc ggcgatggtc
     4621 cccgactatg tcattctcga tgcacgcatg atcaagaacc tgccgcgcaa gatcgccgcg
     4681 gcgagcggcg tggacgccat ggcgcacgct atcgagtgct tcacgagcaa caaggccaac
     4741 ccgttcagcg acacgttcgc gctcgacgcc ttcgaccgca tcctcaacaa catcgagcgc
     4801 gcctgcgatg atcccgaagc gctcgacgcc aagcgtgaga tgcagatcgc cgcgttctac
     4861 ggcggcatcg ccattacctg ctccggcacg acggcggtgc atgcgctgag ctacccgctc
     4921 ggcggcaaat accacatcgc ccacggcgta tcaaacgcca ttctgctcat gccggtcatg
     4981 cgcttcaacg agcccgccat ccgccacaag ctggcgcttg cctacgaccg cgcggtgcac
     5041 ggcgaaaaga catgcgtcac cgacgaggaa aagagcgcct ggatcatcga gcgcatgggc
     5101 gagatcgtaa agcatctcga catcccgaca aacctttccc agtggaacgt ttcgcgcgac
     5161 gatcttgagc cgcttgttga ggcgggcatg agcgtgcagc gtctgctggt gaacaacatg
     5221 cgcaccgtaa cgccggagga cgcgagaaac atttacctcc agatccttta aaaacggaaa
     5281 ggagaaaaaa agatggccca tcccactctt cacggcatcg ttccgcccat catcaccccg
     5341 atgaacgcgg acgagagcat caacatcccc gagctcagaa accagatcga gcggcagatc
     5401 gcgggcggtg ttcacggcat tttctgcttc ggcacgaacg gcgagggcta catcctctcc
     5461 atgaaggaaa aggaagagat cctcgaagcg acgatcgacc aggtcgccgg gcgcgtcccg
     5521 gtgtatgccg gtacgggctg catctccacg gcggacacgg tatatatgag ccgccgcgcc
     5581 gaggaaatgg gcgcggacgt cctctccatc atcacgccga gcttcgctct tgcgagccag
     5641 gaggagctct accgccacta tgtcgaggtg gcaaagcacg tgaatatccc cattgttctc
     5701 tataacatcc cgccgcgcac cggcaacaag ctcctgcccg agaccgttgc gcgtctcgcc
     5761 aaggacgtgg acgtaatctg cggcgcgaag gattccagcg gcgatatcga aaatctcaag
     5821 gcgttcatcc gcgtgggaca agagctcggc cggccgttct cggtgttcgc cggaaacgac
     5881 ggcgcgatcc tcaccggtct gcgcgagggc ggcgcgggcg gcattgccgg aagagccaat
     5941 ctctatccca aaactgtcgc cgcgatctac gacagctttg tcgccggaga catcgcgcgc
     6001 gccgaggaat gccagcagcg tatctcgacg ctccagcgcg tgttcaaatt cggcaacccc
     6061 aacacgatca tcaaaaaggc cgtcaaccta ctcggttacc cggtcggcga gtgccgccgc
     6121 ccgttcaact acctgtgcga cgagggcgtc gctgagctga aagcggtgct gaaggaaaac
     6181 gaagagctcg gtctctgctg aggaggaagc gccatgagca cagtcaaaaa gccgatcctc
     6241 ggcatcacga tgggcgaccc cttcggcaac gggccggaga tcacggtccg ggcgctcgcg
     6301 gacgaaacgg tctatgaccg ctgccgccct ctcgtcgtcg gcgacgccgc ctcgatggag
     6361 tacgccgcgg cagtgtacaa taaactgacc ggcgctgcga tccacgtcca ttcggtgcag
     6421 agcgtgcagg aagcacaatt cgtccccggc acgatcgatg tgctgactct cggcgtcgta
     6481 aaggcggagg acatcccctg cacggcgggg gagccgaagc ccttcggtct cggcgcaacg
     6541 gcgctcggcg gcgaagcggc gttccggtat gtgaagcgcg tcattgagct ggcgctcgcc
     6601 ggcgaggtgg acgcgaccgt cacgaacgct ttgagcaagg aagccatcaa catggcgggc
     6661 caccacttct cgggccacac cgaaatttac gcgcactaca cgaacaccga taaatacacc
     6721 atgatgctcg cgcacgagga tctccgagtc gtccacgtat ccacgcacgt atcgttgcgc
     6781 gaagcgtgcg accgggtgaa gaagccgcgc gtgctcgaag tgatccgcat cgcggacgcc
     6841 ggatgccgcg cactcggcat cgagaagccg cgcgtcgccg tggcggggct caacccacac
     6901 tgcggcgaga acggactctt cggcaccgag gagatcgagg agatccagcc cgccatcgac
     6961 gaggccatgg cggagggcat ctgcatcccg gacaaaaagc ccacgccgcc ggacacggtg
     7021 ttctccaagg ccatcggcgg ctggtacgac atcgtggtgt gtatgtacca cgaccagggc
     7081 cacatccccc tgaaggtcaa gggctttgtg tacaaccgcg aggcgggcca ctgggacgcg
     7141 gtggcgggcg tgaacgtaac gctcggtctg ccgatcatcc gcgcgagcgt ggaccacggc
     7201 acgggcttcg gccacgccgg aagcggcagc gccaacgccc tcagtctcgt gaacgcgatg
     7261 gactacgcca tccgcatggc gaacgcgaaa aaccattgaa actgtcttat ataccggcag
     7321 ataccggttt taagagaaat aaaaaaagga ggcacccaat gaaaaagaca ctggcacttc
     7381 ttctggcagt tctgatgctg gtttctgttt tgtctgcctg cggtactacc gcagagacgc
     7441 ctgccgcacc gactgcggaa gcaacggcgg aaacgatggg aacggaaact ccggaagaag
     7501 aatccgcgct ggaagcgcag gacctgatat ttgcgacgat ggacgtaggc acttcgatct
     7561 atacctactc ttccgcaatc gccaatatcc ttctggagta cctgcccgag ggctccacca
     7621 ttgacgttcc caccacttct ccgggcggtc tcggcgcggc atatttgctg gcgcagggaa
     7681 agagcgacct gattattgtc aacgatgtcg gctttatctc ctcgattgag ggcggcgttc
     7741 taggcgaggc atcgatcgac tccaatctgg ttgccggcat tgtcgggagc atggactggc
     7801 cgcatgtgga cattctcgtc cgtaatgatt atgctgaaaa gaacggcgtg aactccattg
     7861 aggaagtcat tgaaaagaag attccgacaa acttcgtcat caagcaggcg ggaacgatgg
     7921 gtgagctggc gtttgttcaa ctgctcgaag gactcggcgt gacggaggaa gagctgaccg
     7981 gttggggctg cacgatcact cgtactgcgt ccggcaatat caagaccgct atgcaggatg
     8041 gcacgggcga tatcacggtc gaccatccgc ccaacaatca ggcgaacacc gttgagctga
     8101 cgctgaatac cccctgcacg ctttgggatg tccccgaaag cgtccgtgat tacatggagt
     8161 ccaagggctg gaccaaaacg acctggaagg ctggtacctc cggcttcacg ggtcatgacg
     8221 aagatattca gacggtttcc tccagcaacg ttgttgtctg ccgccgcgat ctggacgagg
     8281 aagtcgtcta cctgatcacg aaggcgatct gcgagagcgg cgatgagctc gctgcagcgt
     8341 caagcgctct ggcagagttt gacccgagca tcggctgggt taccatggaa aagtacatgc
     8401 accccggcgc gattcgttac tataaggaaa tgggctacat gccctgacgg gggtcggccg
     8461 cttccttaca gcagcttgta ttcgggagat atacggagca cccgtatatc tcccgagtta
     8521 gaaaggagag accatatgaa agacaagcag atcgatctcc acagggtcat aacggtgatc
     8581 atctccatcg tgtggatggc gtttcagacc tatatcatcc ttggcaaacc gatgaacccc
     8641 atgcttcagg tgcctgttca tctggttttc gctctggcga tcatctatct ttataacccc
     8701 attgacaaga aaaatgaaaa gcttcgtaaa ctgaagttcc tagattatat tttctatgcc
     8761 gggattttgt tccttgcata ctattacatt tgcgaaaccg ttcgtctgca atcgcgtgtg
     8821 ccgtactttt ccgcgttgga gacgattgac atagtagcct tttttgtact gtgtgttgtc
     8881 ctgctggagg cagtccgccg cacgcttggc tggaacctga tcatctttat tgcaattttt
     8941 gtgctatatg ccctttttgg gcagtatctg ccgcgatcgt ccgggctacg ttccacgagc
     9001 attcggcctc tggagttcgt tgaactgatc tccatgagct ccggcggtat tcttggcacg
     9061 ccgctgacga cgtcggcgac ctaccttttc tacttcatga tcttcggcgc gatgttttcc
     9121 acctgcgggg gcggtcagct ccttattgag atcggcctca aattcggcaa aggccatggc
     9181 ggcccggcga aggctgctgt catttcctcc ggcctcctcg gcatggtgag cggctcggcc
     9241 gtcggcaacg tgtccacgac cggcgtcatg accatcccga tgatgaaaaa ggttggctat
     9301 acgccggaac aggcgggcgc tatcgaggca gtggcatcta ccggcggaca gatcatgccg
     9361 cccatcatgg gtattggtgc attcattatg gcagagatgc tcggcgtgca ctatatggaa
     9421 atcgccaagt ctgccattgt tccggcgctg gcatattacc ttgccatttt cctcgttgtt
     9481 gactttatcg cccggaaaaa taccaataat attgaatcga agggctttaa cgttcagcca
     9541 atcctcccgc gcatatatct cctgcttccg gcgatcgttc tggtgtattt catcattacc
     9601 ggtatgtcgc tgatgcgctc ggcggcatat gccatcgcgg ttattctcgt gctgaacatc
     9661 atcaacccca agcgcgcggc gtggaaggac gtatggaacg ccatgctcgt cggctgtaag
     9721 caggcagcta gcattgctat ccccacggca gcggtcggta tcattatcgg cgttgtcgta
     9781 aactccggcc ttgccacgaa gctctcctct ctcatgatga agctcggcag cagcggtatg
     9841 ttccttgcgc tggtgctctc catgatcgga tgcatgctgc tcggtatggc gctgccgacg
     9901 gttgctgcgt acatgatcgc caacatcctc tttgtcccca cgctcacgaa gctcggcgtc
     9961 gaccggctgg cggcaaatat gttcgtgttt tactttggca ttttcgcgca gatcactccg
    10021 ccggtgtgcc ttgcgtcgtt caccgcggcc ggtatcgctg gcggcgattc gtggaaaacg
    10081 ggctggacag gcttccggta ctcgattgtg gcgttccttg caccgtatgc cttcgtgtat
    10141 aatactgcga ttctcttgat cggctctgta ccggatatca ttcaggccgc ggcgcaggtg
    10201 ttccttggaa cgttcttcct tgccgcgggc gtggaaggct ttgtcggaaa accggtgaaa
    10261 aacattgctt tgcgcattct gctggttgcg gcgggcgcac ttatcattat tccagaggtc
    10321 attacgacgt tggcgggata tgcgcttggc atcggcatcg ttgttgccgt gaaccttctt
    10381 gcccaacgaa aagagggaaa gaaggataag gcgtccgagc tccggctggc agtcacagcg
    10441 gtctccggtg cgctggtcgt aacgccggag atcattacga cggttgcggg gctttgcctg
    10501 gcgctggcca tgctcatctg gagcaagcgc gccgagaaga aacagaagaa agacgtcgta
    10561 tacgtggaag ccggagaggt tctggagacg gttgcacagg atatcgacga ggactgagct
    10621 atggcgttgt caataggtat ctgcacaaac ttcctcggag aggatcgcta ctctcttcaa
    10681 acagaacggt atcccgaaat atcccgggtt ttcgactatg tcgagcttcc cgccatgacg
    10741 gtcacagcgc tcagcgatcc ggaatttgac gcgctgagag cagagctcga aaaggtgggc
    10801 ctacgctgtc cggttatgac gaacctgttt ccggcggaca tcccgctgct ttcaccgtcg
    10861 ctggatgccg aagcgctgaa agcgtatctg gacgtattgc ttccgcgatg ccgggcactt
    10921 ggctgttccg agctcgtgct cggcagcgga aaggctcgta gccttcgtcc cgaacaggac
    10981 gcagaagaag gatatacgcg cctggcggag cttttgaatc ggaaagtgct tcccactgcg
    11041 tccgaatatg ggattcgaat cattctggag ccgctgaatc cgttgatctg caactttatc
    11101 ttgacgcttg gagaaggtgc ggagctctgc cgccggtgcg acggccaact tgcgcttctg
    11161 gcagattcac tgcatctgat gtatcaggaa aacatagccg gagagatcct gcggtaccgc
    11221 gggcagatta cccatgtcca cctgtctgaa cccgggcgca aagcgccaga aacaaacgcc
    11281 tcccctgctc tgacagcgtt ccttcgtgcg cttaagaccg ccaaatacga cggaagcgtg
    11341 agctttgagt gccggttccc ggacggcaaa agcatgcagc agggacgggc tgtgctgcaa
    11401 accttgtggg ataaactgta aggagaacaa agaatgtata cttcattgaa aggcaaaacc
    11461 gccattgtca ccggcgccag ccgggggatt ggtcggggga tcgctctcgc tcttgcgtcg
    11521 gagggtgtga atctcgtact caacgcgacg aagcccgctt cgctggaaga ggtgcgcatg
    11581 gaggtcaagg cgctgggcgt gtccttcgac tgcgttgccg gcgatatggc cgacccggaa
    11641 acggcgcgac gccttgccgc gcttgccatg gagaagcatg gccggatcga cattctggtg
    11701 aacaacgccg ggatccagaa tcgaaaaaaa gttgccgagc tctcgcttga ggaatgggac
    11761 cggatgattg cagtgaacga gagcggtgtt ttctacatgt gcaaagccgt gcttccgttt
    11821 atgacgaaag cgatgcaagg cagcattatc aatattacct ctactgcaag caaaacaccg
    11881 caccccaacg cggccatctg ctatggagcg tcaaaggcgg cggtcaccta tcttacaaag
    11941 cacctcgcca tggagctcgg gccgttgggc atccgggtca atgccgtgca gtgtggcccc
    12001 atccgctcgg atatgaccga ccagtggacg gacgaatacc gtgccgccgt gatgaaaaag
    12061 ataccgctcg gcagattggg aacccctgag gacgtggcgg caggtgttgt gttccttgcc
    12121 tcggatgagg cgtcgtggat caccggatgc agtcttaact gcagcggagg aaagtataca
    12181 gaataaacgg aggaaagccg atgaactacc cggtgttgca ggagctgccg tttgacggta
    12241 cgatctatcg gaacgggcac atgaacacgc tcgaagcggt gctgccgccg ggagcatata
    12301 cgtcacacca ctcccctgcg ctgttggagc tgccggacgg cgaccagctt tgcgcgtggt
    12361 tcgccggaac atatgagggg aatacggatg tgggcattgt ctgctcccgg cttccgttcg
    12421 ggagcgggaa atggacggag ccacagccca tctcgcacga cgcggagcgc agcgagcaga
    12481 acccctcact ctttctcgct ccgaacggcg aggtatgggc gatgtacact gcacagctcg
    12541 cccgcacgga gggtaaggac aacatgcagt atacctccgt cgtgcgccgc cagaagagca
    12601 cagacggcgg aaaaacgtgg agcgattacg acgttgtgtt cccgcaggag ggaacgttct
    12661 gccgccagcc aattcagatc ctctccaacg ggcggtggat cttcggcaac tggatctgta
    12721 ccgacagcga aaatggtctt gcgggtgacc ccacggtgtt ccagctctcc gacgatgaag
    12781 gaaaaacctg gcgcacggtg gggatgccgg gaagccaggg gcgcgtccat gcgaatgtgg
    12841 tcgagctcga agaaggccat ctagcggcat tcatgcgcag ccgcgatgcc gaccgcattt
    12901 accgcagcga gtcgctggac tttggtgaga cgtggagcgt cccgaagccg acgccgctgc
    12961 cgaacaataa ctccagcatc agcgccgtaa aactgcgcag cggaagagta gccattgcgt
    13021 ataaccccac atgtacgccc tgcccggagc cgggcaaggc ggcatggccg gggcttcgct
    13081 gcccggtggc ggtggcgctg tccgaggacg gaggaaagac gtttccactc atccggcata
    13141 tggagctggg cgaggggttc gtcggcgcag aaaactctac gaacaaccgc acgtatgagt
    13201 atccgtacct gatgcagggg cgagacggac ggctgtatct tgcctttgcc tacagcacgc
    13261 gccgcagcgt caagtggatg agcttcaccg aagaggacgt tgccggggca aagcgcgaag
    13321 tgatcgggct ttataacccg acggcggcaa aaatgagcta aggaaaggct atgaaaaaca
    13381 cgtttactct cgcacatgtc ggcgtcaaca ccgaaaacgc cgaagaagcg ctcgcgctcg
    13441 cggagctttt ggccggcatg ttcaatctcg cgccgcgcca cgggcagaag tccgagttcg
    13501 ccgggctgta tttcgagtgc atgaaggcgc cgtttcacgg caaaaacggt cacatcgcca
    13561 tgcagacacc ggatctggaa agcgccgttg ccgagctcaa agaaaagggc ttttccttcc
    13621 gtgaggagac cgcggcgcac gacgacgccg ggaagctcaa aaacatttat ctcgccgggg
    13681 aatacggcgg cttcgccatc cacatcctcc ggggctgacg acaacttctt tatcaaagct
    13741 gcgaaagcgt catccgcaag gatggcgctt tcgctatgct ctgcggtgcg gcaggaaaac
    13801 gctttcgctt tttttcgttg accgattttt ggcaggtata tatactgagc tcaacaaaag
    13861 gcgagttcct tttgtgacaa aacaataaag gagccgaaac gatgaagaag atccttgcac
    13921 tgctgatggc agtatgtatg atgcttgccc tgctgaccgg ctgcggcagc gccccggccg
    13981 aaacgccgca gaccccggcg gaacagcccg tcgcggaaaa acctgcagag aaggaaaccg
    14041 tcgcgctcaa ggtgtgggcc gatcaggtcg agctggagct gacggaaaag ctctgcaacg
    14101 attttgccgc tgcgcacccg gaaaaggaat acgtgtttga gttcggcgcc gtcggcgccg
    14161 tagacggcaa gagccgctat ctcgaagacc ccgcggccgc cgcggacgtg ttcgtgtttg
    14221 ccgatgacca gctcatcgat ctcgtgaagg cggatgcgct ctatgaagtg acccgcaaca
    14281 ccgacgcgat cgtcgcggcg aataccgccg gttcgatcaa cgccgcgtcc tacgacggcg
    14341 tgctctacgg ttatcccatg acctccgata acggctactt cctgtactac gacaagtcgg
    14401 tcttcaccga ggaagacctc aaaacgctcg atggcatcct cgccgcggcc aacgccgccc
    14461 gcaagcaggt acatatggac gtctccaacg gctggtatct tgcatccttc ttccttggca
    14521 acggctgcac gctgaccatc gaggacggca agcaggtcat ggacttcaac agccccaacg
    14581 gtctcgccgc tgccgaagcg atccgtgaat tctgcaacga ccccgcgttc gtgaccggtg
    14641 acgactccgt cctctccggc ggcatcggcg attccatcgc ctgcggcgtg tccggcacgt
    14701 gggtcgccac cgcgatccag gaaaagctcg gcgaaaacta cggctgctgc aagctgccga
    14761 cctttacctg cgacggcaag caggtgcaga tgggctgctt cctcggctgc aagatctacg
    14821 gcgtcaactc ccagacggct tacccggtgg acgccatgga gctcgcggag ttcctgacct
    14881 ccgaacagtc gcagctcgag cgctatgaag cgctcaacta cggcccgtcc aacgttgccg
    14941 cgcttgcgag cgatacggtc gcgtccaacc tcgcgctgcg cgctcttgcc gagcagagca
    15001 actacgccgt gacgcagctc gtcctcggcg gcttctgggt cccggcggag gcgttcggcg
    15061 ccgagctcga ggcgcatacc accgccgatc tgcagacgct gctcgatcag ctggtggagc
    15121 aggcgaccgc agcctgatat aagacggtaa cgcacggata aaaggggctg gcaacagccc
    15181 cttttccggg tttcatcttc cggtttttcc ggaccaaacc gagggatagc ggaggtgcgc
    15241 gatgcacaga gaaaaacgtc acatacttct ggatgccgcg gcgagaggcg gtctttggac
    15301 gcgcctgtcg tttctcatca tgggcgcggg ctgcatccgg tataagcagt tcgtccgcgg
    15361 cgcggcgtat ctgctcagcg agatcgggtt tattttcttc ttccgggatt ttgcgatgca
    15421 gtatctctcc aagtttgcaa cgctcggcac ggagacgcag cgcaaggtat ggaacgagga
    15481 gctgcggatc ttccagcgct tccccggcga taactccatg ctgattttgc tcttcagcgt
    15541 gctctcgatc gcggtgtgcg ccgtgtttct ctatctctgg tattccaaca tccgggacgc
    15601 gtggcgcgcg caggagaaca ccgagcacgg ctgcgcgatc gcttccttcc gggacgatct
    15661 ccgcgccatg cggcacgaga aattccatgt gacgctgctc gcgctcccga tgctcacaat
    15721 ggtcctcttc acggtgctgc cgatcgtctt tatgatcctc attgccttca cgaactttga
    15781 tgctgaccac cagccccccg gaaacctgtt cacgtgggtc ggcatgtcga acgtgaccga
    15841 catcttcctc ggcaatgcga tcaagacgcg cacgtttttc ggtctgctcg gctggacgct
    15901 cgtgtgggcg gttctcgcca cattcaccaa ctactttttc gggatgctgc tggcggttgc
    15961 catcaacagc aagctcgtaa agctcaagca ggtctggcgc acctgcttcg tcatcgccat
    16021 tgccataccg cagtttgtaa gtctcctgct catctcccgc gcgctggaac cggcaggcgc
    16081 ggtgaacgtg cttcttatga agctcggcat tatccagacg ccgctgccgt tccttctgga
    16141 cccgacgctc gcgcgcatcg tcgcggtcgt ggtcaatatg tggatcggca tcccgtattc
    16201 catgatcacc ttctcgggcg tgctgatgaa catcccggcg gagctctacg agagcgccga
    16261 gatcgacggc gcgggtccca tgcggaaatt catttccatt acgctgccct acatgttctt
    16321 cgtcaccgcc ccggcgacga tcacgacgtt cgtcacgaac ttcaataact tcaacgtcat
    16381 ctatcttctc accgccggcg ggccgttctc gctgggctat taccaggctg gcaagaccga
    16441 cctgctcgtt acgtggctct ataagctgac cgtgaaccgc aacgactatt cgcttgcctc
    16501 caccatcggc atattcatct tcctgctggt gggcacctgc tcgctgatcg tatacaaccg
    16561 gaccggagcc gtaacgaagg aggatcagtt ccaatgaaaa atgcacgcat ccgccgccgt
    16621 gtttcggccg tggcgctcaa cgcgcttctt gcggtccttg ccgttttgtg gctcgtgccg
    16681 gtgttctggc tgattgtttc gtccttccgg gcggagcagg gcgcgtatac gccctatctc
    16741 tggccgaagg cgtttacctt tgataattat aaccgcctct ttaccgaccg gaggttattc
    16801 gacttcccgc gctggctcgg caacacgctc ctcgtcgccg ggtgcagctg cgctctcaca
    16861 acgatgctca cgctgatggt ggcgtatgtc tattcccggc tgcgcttccc gaagcgccgg
    16921 tttcttatga acgcctcgct cgtgctgggc atgttccccg gctttatgag catcatcgcc
    16981 atttaccact ttatgaaggc catcggtctg gatcaaacgc tgcttgctct gatcctcgtc
    17041 tattccggcg gcgcggcgct caattactac atcgccaagg gctattttga caccatcccc
    17101 aaatcgctgg acgaagcggc gatcctcgac ggcgcctcga acagcacggt gttctggaag
    17161 atcatcctgc cgaactccaa gcccgtcatc gccacaaccg ccatcaacgc ctttataaac
    17221 cccatcgtcg attacatttt cgtctcggtc attatgaagg acaattataa taacttcacc
    17281 gtcgccaagg gcctttacac catggtggac aagacgaaca tttatgaata ctacaccgtt
    17341 ttctgcgcgg gcgctgtcat tgtggcgatc ccggtcgtgc tgctgttcct ccgcgtgcag
    17401 aaatactatg ttgccggcgt tgtcggcggc tccgtgaaag gataagctat gaatcctgca
    17461 acgatatttc accggccgat gagcgaatac gccttcgcgc tggacgacac gcactatata
    17521 ttccgcctgc gcaccggaaa gggcgaagcg gagagcgttc ggttttacta tgccgaccgc
    17581 gccgtgatga cccccaaact gcaatttgcc ccgctgccga tggagaaatt ccggacggat
    17641 cggtattttg actggtacga gatccggctc gaaacgcgct tcgagcgcat cgcctactat
    17701 tttgaactcc aaaacggcgt ggaaacgctc ttctactacg gcgactgcta cgaaatggca
    17761 ggcacgccca cgcgcgcgga ctatttccag ctcccgttca accaccgcgc cgaccggttc
    17821 gcggctccgg cgtggacgcg ggacgccgtg gtatacaaca tcttccccga cagcttcgct
    17881 gcggggaagc gcctggcgcc caacggcgcg ccgccctgcc ggggcgggac ggtgcgcggg
    17941 gtgaccgaaa atctcgacta catcgcctcc ctgggcttca actgcattta cctgaacccg
    18001 atcttcgcgg cgagatcgta ccaccggtac gatacgctgg attattatcg catcgatccg
    18061 cacatgggcg ccgaggacga tctccgggat cttgtccgcc gcgcgcacgc gctcggcatc
    18121 cgggtcattc tcgacggcgt attcaatcat gtgagcagcg atcatccctt cttccgcgac
    18181 gtgctggaaa aggggcgggc ttcgcggtac tattcctgtt tctacgcgct gccggagacg
    18241 ccgcgcctgc ccgccgccgg ggnnnnnnnn nngccgggga gctgcccggc tatacctgct
    18301 tttcgtacgt cgccgatatg ccgaaaacga acaccgccga ccccttcctc cggcagtatt
    18361 tctgcgacgt cggcgcgaac tgggtgcgaa agtttgacgt ggacggctgg cggctggatg
    18421 tggcgaacga gctggacgac ggtttcctgc gcgcgttccg cgcttccgtc aaggcggcga
    18481 agagcgatgc gctcatcgtc ggcgaggtat gggaaaatgc cgcgcattat ctcggcggcg
    18541 acatgctcga ctccgcgatg aactacgatt tccgccgcta ctgccgccgg ttttttgccg
    18601 agcagacggt ggacgcagag accttcgata cgaatgtgtc caccctgctg ctgcgctata
    18661 acgaaaacgc cctcttcgcc cagctcaacc tgctcgacag ccacgatgtg agccggttcc
    18721 tctcgctctg cggcgggaaa accgagcgga tggagcttgc ggtgctgctg caaatgacgt
    18781 tccccggcat gccgtgcgtg ttctacggcg acgagaaggg gctttgcggc gagagcgagc
    18841 cggaataccg ccgcccgatg gcgtgggacg cgagctctcc gctcgaggag gtgtatcgcc
    18901 ggatgatcgc gctgcgaaag acgcacccgg cactgcggta cggcagcttc catacggagc
    18961 ttgcctgcgg cggggtctac cgatacagcc gcgtctggaa cggtacgaag atcaccgttg
    19021 cgatgaatct tggcgccgag ccggtcaagg ccgaaaagcg cgggacgctt ctcttgaaaa
    19081 agggagaaaa ccgtgacata atcggcgcat gggaatacga ggtatgggag gaacaaaacg
    19141 acggcggtaa cgatctatga tgtggcgcgc gaggcgggtg tctccatcgc cacggtctcc
    19201 aaggcgctga acgacagcag caccatttcc gagcatacaa aggaacgcat ccgggagacc
    19261 gccgagcgtc tcggctaccg cccaaacgcg cgggcgcaga gttttgcgcg ccaggcgagc
    19321 gggacagtgt tcttccttgc ggacttttac cggaacattg cgtttgaaaa cccgcacatg
    19381 tttgagatca tctccggcac agccgcgtat ctcgaaaaga aggggtactc gctccttttc
    19441 aagccgcttt cccggcagga agcgcccaag gtcatccgcg acattatcca ggagcggcag
    19501 gcggacgcgc tgatcctcca cgcgcacatc ctcacggcag agctcgcggc gatcctcacg
    19561 cgcgtcgatt ttccgtatct cgtgatcggc aagccgaacc agcgcagcag cgtttgctgg
    19621 atggacgtga accacgagca ggcggggcag atcgccgcaa actacctgct tgataagggc
    19681 taccgccata tggcgttcat catgggccgc gaggacgacg ccatcgcgcg cagccgtctg
    19741 accggtatcc gccaggcgat ggacgaggag gagctggccg tggaggtcgt ttgcggcagc
    19801 tctccgtgcg atctggatga cgcggcgctc gacgagctgc tctcccgccg cgtgcggccg
    19861 gaggtgatgc tctgcgcaaa caaccatctt gcgctgcact gtctgcaata catccgccgc
    19921 cggggactga agatcccgca ggacgtggcg ctgctgacct ttgataatta tccgttttcc
    19981 atgctcatgg acccgacggt caccgccgtg gaggttgata tgtacgatat gggcgtcaac
    20041 gccgcgcgct ttgtcctcca gcgcatccgc aagccgaacc tgcggacgca gtcgtatgta
    20101 acgtcgccgt gcatcctcga acgggaatcg acgtaaaaag gctccctcgt tgagggagct
    20161 ggcagcggca aaactgcaga ctgagggatt gtagtctgcg gaaggagaca atccctccgt
    20221 caaaatcttc ggttttgcca cctcccttta cacaagggag gcatgaaaaa agctccatcc
    20281 ggacggatgg agctttttga tattcggtat agatcgatca gcgcttgctg aactgcggag
    20341 cgcggcgggc ggctttgagg ccgtactgct ttctgtctta cgggcgaaaa ccgttgattt
    20401 tactgggttt tttaggtttt c
//
LOCUS       DAWAAE010000020        19136 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1289_length_19136_cov_2.926262, whole genome shotgun sequence.
ACCESSION   DAWAAE010000020 DAWAAE010000000
VERSION     DAWAAE010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19136)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_19136_cov_2.926262"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2145..2154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7809..7818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10314..10323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12040..12049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12744..12753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16481..16490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3859 a         5885 c         5502 g         3830 t
ORIGIN      
        1 cgcagcgctt ctttctggac ggcaacacga ggatgcactt ttcgctgccg gggctctttt
       61 acgttttgag cggtattctc tggttcatcc ccgttatctg gctgctgctg ttcgcgctgg
      121 aatggctctc cgcccgcccc cgcacgctgc cgaaaccggg aagccggaag aaggcgctgt
      181 gcgttcttct tatcgcgctg cttctctgtc aggtctttgt gctctgggtc gaatggccgg
      241 gcggatttcc gctcgacgcc gtttatcaat accgtcaggc gatgggtgag ctcccgctga
      301 gcgacgatca tccggtgctg gatacgctgc tcatgcgtgc tgttctgacg gtctttccgc
      361 atcccgccgc gctcacggcg gtgcagctgc tgcttttctc gctcgtgctg tgcgcgttcc
      421 tcatgatggg gtacgaccgc ggcatgccgc tgccgctgct cgcctttttc gggtgcttct
      481 tcgagctgat gccgaatcag gtgctcagct ggacgaacgt tctcaaggac ttcccgttca
      541 cgttcgcgct tttgtggggt acatatctcc ttttgcagct tgcgctggat acgacacgca
      601 gccgggaatg gccgttttac gtctgtcttg cgctggacat ctttctcgtg tccctcctgc
      661 ggcacaacgg cgccgcgccc gcggcggcga tgatcgttct ctgtattgtg ctcaccgtcc
      721 ggcgctatga gcaggtgaag ctccgcgcgc ttaccgcggc ggtgcttgcc gcggtgtcgt
      781 tcggcgtatt caaggggccg gtcatgacgg cactcaatgt tgcgccggtg tcgctctcgc
      841 cgttcacgac gcttttctgc gccgtcggtt cgtgcgtgaa taaggatctg ccgctctccg
      901 aggaagcgga cgcgattttg gaaaaggcgc tcccgctcga ggactggcac gattattacg
      961 cccgctatta cggccatgat ttctattact ggggccgtcc ggaggggagc gttccctata
     1021 cgccaacgga ggtaacgacg ccggatatcc tccgggtcta cggcgaagcg ctatgcaagt
     1081 atccggatgt gatactaaag gaccgcatcg acgggtgcga cattctctgg gatgttgcgg
     1141 aaccggagga cagcttcaac gcaaagtcct tctgtcttgt gtatgaccgg gactggatgg
     1201 cgcagtacgt cgatatggcc gattactacg atgcggacga cggcgtgctc cgcgattacc
     1261 ggcagaactt cttcaccaaa gcctacctcc ggctgaccgg aacgccccga aacagcgtgg
     1321 tggacattct gctctggcgc acgggcgcgt atctgatcgc gttctgggtg ctgctgctct
     1381 tctggcggaa aaacggtatg cagcgtttcc gctccgcagc cgtgccgttc ctcggcaatc
     1441 ttatcatctc cgtgctgtat gtttaccacc agagcttccg gtacgtctac ttcgtgcagg
     1501 tgtgcgtgct cgcgctcctc ttcgccacgg tcgtgtgcag accgcggccg ccgcagccgg
     1561 aagaagaata aaaaaagacc gccgcgtttc cggtgtttcg gaaacgcggc ggtcgatttt
     1621 ctcaggagtc ttttttatcg acgcggtatt ttttcttgaa gatgctcgcg cggatgccga
     1681 gctccttgcc cttgacgagg cgggcgaagt tcttccggtg cttccagaag atgagcgcga
     1741 ccgtggcggc catgatcgca atgtcccagc cgctccgtcc cgtgaggatg caccagacga
     1801 gataccccac gctcgccgag agcgtgccga tgaacatata gtccgtgatg aacgtgatga
     1861 ccgcgacgcc cgccacaagg atgagaaaca gccgccagtc gagcgcgagc gcgctgccga
     1921 gcagcaccgc gatgcccttg ccgccctgga attccagata gaacggatag agatgccccg
     1981 ccacgcacgc cgccatcgtc acggtctgga tggagggcga gctgtggaac aggaatcccg
     2041 ccagccatac catgagcgcc gatttgagcg cgtcgcacag aaacacgacc gccgcaacgc
     2101 ccgagcccat cgtccgcccg gcgttcgtcg tcccggcgtt gccgnnnnnn nnnnttgccg
     2161 gagcccttcg tgcggatatc aaagccgcgc ttcttcgcca gcagccacgc aaaattcacc
     2221 gctccggcca gatagcccgc gagaatgcag aacacatacg gaagaacggt tttcaaaaaa
     2281 gtctgcatga atgcgatcct ttcgcagata cataaagaaa tttatatact ttggcatact
     2341 actatgtgat atcacagtat aaagattttg tcaacgaaca tatccgcgct attgtctttt
     2401 tcggcagaaa ggtacttgcg tttccgcgcc tcagcgggta aactgtatct gtatttaaat
     2461 tatgtaaccc aattcaatac ggaggtagta tatgtactgc acgaaaaaaa ttgcgtcgga
     2521 tcttgtctgg gtcggcgcga acgaccgccg tctcgccatg tttgagggcg tatattccgt
     2581 tccggcgggc gtttcctata actcctatct tctcacggac gagaagaccg tcctcttcga
     2641 cacggtcgat aaggccgtga gccgcacctt ctttgaaaat ctggagcacg agctcggcgg
     2701 gcggccgctc gactatgtga tcgtacacca catggagccg gaccactccg ccacgctctc
     2761 ggagctgctg ctgcgccacc cggaaaccac ggtcgtctgc aacgacaaga ccgccgtgat
     2821 gatcgcgcag tttttcggca aggacgccgt gaagaacatg cagctcgtca aggagggcga
     2881 cgtgctctcc gctggcgcgc acgagctcac gttctatctt gccccgatga tccactggcc
     2941 ggaggtcatg atgacctacg ataagagcgc gcaaacgctc ttcaccgcgg acgccttcgg
     3001 cgttttcggc gcgctcaacg gcgcgatctt cgccgacgag gtggattttg accgcgacta
     3061 tctcaaggag gcgcgccggt actatacgaa catcgtcggc aaatacggcg tgcaggtgca
     3121 gaatctcttc aagaagatcg cgggtctgga cattcagatg ctctgcccgc tgcacggctt
     3181 cgtctggcgg cgtgatctcg gatatattct ggaaaagtac aacctctgga gcacctatac
     3241 gccggaggac aagggcgtga tgatcgcgta cgcctcgatc tacggcaaca cggaaaacgc
     3301 cgccgagatc ctcgcctcga agctgcgcga gcgcggtgtg cgcaccgcga tgtacgacgt
     3361 gtccgtcacc ccggcctcag acatcatcgc cgagtgcttc cgctggagtc atctcgtgtt
     3421 cgcctcggcg acgtacaacg gcggcatctt cgtcacgatg gacgagctgc tgcgcgatat
     3481 cgccgcgcac aatctccaga agcgcaccgt cgcttttatg gaaaacggca cgtgggcgcc
     3541 cgtcagcgcg cggcagatgg cggcgatcct cgctccgctc aagaacatga cggtgctcga
     3601 gcagagcgtc gcgatccgct cctcgctcac cgaagggcag ctcgccgaga tcgaagcgct
     3661 cgccgatgtg atcgcggagg gcatccggta aaaactggca acgaaaaata acaaaaattc
     3721 cgagtgcgga ggaaaccttt tgaccccgcc gcgcgtctta tagtcagaaa gcaagcggca
     3781 gggctatgca ccgcgccgtt ttcaaggagg tactttgtta tgacaaagaa actgatggct
     3841 cttctgctcg ccgtgctgat ggcagtatcg ctgctgccca tgccggcgat ggcaggcgac
     3901 gcccaagtca tcacggacct ttcgggcatt tccgcgggag gatcctatgt tgtcgataaa
     3961 tccgtcaagg atatcgtcct ctccgcgccg cttacgctcg acaaggacgt gacgctcgag
     4021 ctgaccggtc agacggtcac gctcaagctc gccgacggcg agagctatgc cgacgcttcc
     4081 gttttccatg tggagagcgg cagtctgacg ctcaagggct ccggcacggt caaggtgccg
     4141 gataacgcct ccggcgtcgt tgtcgcgaag gacgccgccg tgaccatctc gggcgaggtc
     4201 ggcatctccg gcggcgcttc cggcgtgaag gccgttgtcg gcgcttccgg cgtgaagatc
     4261 cgcgtgaata cgctcggcaa gatcgagtcg gcaaacggca tcaacctgtc cggcgcttcc
     4321 ggcgagatca aaatcgcctc cggcacggtc aagggcagcg tctgcgccat tgcccccgat
     4381 aaggacaacg cgctcacgct cggcaagatg ctcgacggct ccacgctcta catcggcggc
     4441 acggccgcca cggaggagca gctcgccgcc gctcccaccg ccgaaatgaa ggagcttgtt
     4501 tttgacgacg gcaaagccct cgcgctgact atttcctccg ccgtgcgcac ctcggagacc
     4561 gagggcagcg tcaccttcac cgcctcgaaa gccggtacat actactacca gctcgacggc
     4621 gaacccgctt ccgcggcgga catcatgaag gacgaggcga aggccgagct cgtccgcggc
     4681 gcgaatacga tctctctcaa ggatctcgac gccaacgccc acaccgtcta cattgccgcg
     4741 aaggacgcct tcggccatac gaccgagctt ctgaccgcag cgatccccgc ggtgctcgcc
     4801 gcgccgaccg cccccgtctg ggacggcaag aaggccgcat ggaccggcgt ggagggtgtg
     4861 aatgattaca tcgtgcagct ctatctgggc tccgacgcct acggcgatcc catccccgtg
     4921 aatggtgcgt ccgctacgga tgatctctcc gacgtgatga aggacgacgg cgtttacacg
     4981 ttcgccgtgc gttccgtcgg cgcgagcacc gtcggcgcgt ggtctgagcg cagcgccgaa
     5041 acggtgcgcg acaccgccgc ccccatcctc acggtgccgg ccgacggcgt caagcgcacg
     5101 gcgcaggaca ccgccaccgt cgccttcgtc tccaacgagg acggcaagct ttactatgtt
     5161 ctcaacgacg atgccgccga tgtattcacc tccggcagca ccatggcgct cacgaagggc
     5221 gaggacaaca cgctcacgct caccggcctt gccgacagcg ccgccgtcgt cgtccgctac
     5281 gccgccgagg acggtctcgg aaaccgcggc gaagtgcaga ccgtgaccgt gccgctctat
     5341 ctcgcggccc ccgccacgct cacctgggtg aacggcacct ccaccgcgat gtggtcggaa
     5401 gtccccggcg ctgcgtccta ctgcgtgcag ctctataagg acggcgcgga ggtcacgcct
     5461 gccgttaccg cggatacgac cagctacacc ttcacgctgg aagagtccgg ctcctacacc
     5521 ttcaaggtgc aggccgtcaa cggcgatatc ctgagcgcgt ggtcggaagc gagcggcgct
     5581 ctcgccatcg ataagaccgc gcccgccatc tccggcgaga gcgcgagccg cacggacgct
     5641 tccaacggct ccgtgacctt cacctccgac gaggccggca aggcctacta catcgtcggc
     5701 ggcgagaagc ctacgcagga tgctcttctt gcctcggcaa acgtgaagga catcgcctcc
     5761 ggcgaaacga agatcgatct ctccggtctc ggcgcggaag caacgaacgt gtatcttatg
     5821 gttgtggacg ccgccgggaa caggagcgac gttaagaccg tgaaagttcc cgtgtatctc
     5881 gcaaagccca ccacgatcac ctgggtcaac aacaccgcga ccgccatgtg gaatgccgtg
     5941 tccggcgcgg aagcctataa catccagctt ctccgcgacg gcagcgatta cggcaacgtg
     6001 atcgtcgtca acggcggctc cgttacgacc tcggaccttg cgccccacat gaacgacgac
     6061 ggcgtttaca ccttccgcgt gcaggcggcc gccgccggaa cgcagagcga gtggtcggac
     6121 gcttccgcca cgagctataa gcgcgacacc cagaagccca ccatcaaggg cgagccctcc
     6181 aagcgcatcg acgcgaagac cgcggagttc tactttacct cctccgaaga tggaacgtat
     6241 tactacatgg ttggcagcgt gaacggcggt gttccgaccg cggagcagat cgccgacgac
     6301 aagaacccgc acggcggctg caccaacgcc cgcactacca ttaagctcag cgatatcgcc
     6361 gatacgaacg cccgcaaggt ctatgtcgtt gtgcgcgata agagcggcaa tctgagcgac
     6421 gtgttcacga tcaccgttcc cgcctacagc gcgcagccga cgcccacccc gacgcctacc
     6481 ccgacagcca cgcccacgag cgctcccaag acctacaccg tgacgctcca gggcggcacc
     6541 ggctatacca ttgccgcgac cggcggctcc aagagccccg taaacgccgg cggcagcttc
     6601 tccttcaccg taacgcccgg caacggctac acccgcggca acggcttttc cgtaaaggcc
     6661 aacggcacga cgctcacgag caacaacggc gtttacacca tcagcaacat caacgccaac
     6721 cagaccgtca gcgtgtccgg cattgtgaag agacagaaca ccggcggcgg cacgctgccc
     6781 gccgcgcccg ccatcaccac cacgacgctc gccgcggcca cgatgggcaa ggaataccgc
     6841 cagcagatca ccgccaccgg cggctcgccg atcacctggt cctacagcgg cacgctgccc
     6901 gacggcatga cgctcgcggc caacaccggt attctctccg gcacgcccac gcaggaaggc
     6961 tccttccgct tcgccgtgaa ggcgacgaac tccaccggct tctccacgcg gcagatgacg
     7021 ctcgtcgtcg ccggcagtga gtacaccgtc accaaaggcg cgaacagcga atggacgcag
     7081 ggcggagaca agggcatcga cttctccggc agcggcaagg gaaccttcac cgtgaaggtg
     7141 gacggcgccg cggtcgccgc cgggaaatac accgcctccg cagacggctc caccgtcacg
     7201 ctcaagccgg aatatctcga cacgctcgcc gctggctccc acaccgtgac gctcgtctac
     7261 ggcgacggca gcgcgaaggc caagttcacc atcaaggcga aggataagac cgtcgccccg
     7321 accgtttcct cccagcccgc aagcgcgtcc gtcaacgcgg acagctccgc cacgttcacc
     7381 gtgacggcga gcggcaccac gccgctgctc tgccagtggc aggtggacaa aaacgacggc
     7441 agcggctgga cggatatctc cggcgcggtg aacgcgagct acaccgtcga aaaagtcacc
     7501 gccgagcaga acggctggaa gtaccgctgc gtgatcaaaa acgctgccgg ttccgttgag
     7561 agcaacgccg cgacgctcac cgtgaaggaa gccatcggcg atgtgaagaa aaacgacgac
     7621 accaaggaca ccgcggcgtc cggcggtctc ggccgcatcc ttctcatcac gggcatcatc
     7681 gtggccgtcc tggcactcgg cgcgggtctg tacttctact tccgccgccg cagcgcctcc
     7741 cgctacacgg aggacgatac cgcctggaga aagtgatccg gaaataaacc aatacgcaag
     7801 ggccgcccnn nnnnnnnnta gacttttttc tgtcggcctt ttaccgggcc gtgaaaccgt
     7861 aaatctgtgc agtatgcggc gggatcgggt gcggaagggt gcttcgcttg gcattatgaa
     7921 taatatttat tttaccgctt gacattttcg gatttatgaa ttaatattca atcacaagtt
     7981 aaatatatcg ccttccgcat caccgggtgc gggtcaaaaa ggtgtccggg caattccgca
     8041 ggtacggcgc acatgcgcta caaggaatgc cccggacgcc ttttgcttta tccgggctgc
     8101 agcgggcgga aaggagaatt tttatgtaca tcaagccgtt tgccgtagag gaatggatga
     8161 acgaatggga ggtcggcgcg cgatacaaca tcgcggagac atgcgtcgat tccgtgtcgc
     8221 tcaacgagct gttcgcgctg accggcgagg acaaggccgc ctttctggag aatctcgccg
     8281 cgcgccggct gacctacggc gacattgaag gcgcgcccgc gttccggcag ggcatctgct
     8341 cgctttataa aacggtcaag cccgaaaaca ttgtcacaac gcacggcgcg gcgggcgcga
     8401 accaccatgt gttctgctcg ctcgtatcgc cgggtgacct cgtcatcagc gttctgccga
     8461 cgtaccagca gctctactcc atccccgaat cgctcggcgc aaaggtaaag attctccccc
     8521 tgagccgcga aaacgggtat ctccccgatc ttgacgcgct gcgcgcgctc gccgtgccgg
     8581 ggacaaaaat gatctgcatc aacaacccca acaatccgac cggcgcgctc atgagccgcg
     8641 atatgctcga agagatcgtg cagatcgcgc gcgaggcggg cgcgtacatt ctctgcgacg
     8701 aggtataccg ccatctctcc cagaccgacg agtggagcga gtccatcgcg gatctttacg
     8761 aaaagggcat ctccgtgagc agcatgtcaa aggtcttctc tcttgccggg ctgcggcttg
     8821 gctggatcgc cacgcgcgac aaggacgcgc tccacgcgct cgtctctcac cgtgattacg
     8881 atctcatcag cggcggcatg atcgacgagg ccattgccgc gctcgcgctc aagcacagcg
     8941 ataaaatgct cgcgcgcagc cgcgccatcg tgcgggaaaa tctcgccatt ctggacaatt
     9001 gggtcgccgg agaaaagcac gcgagctacg tcaagcccct cggcggcacg acggcgctcg
     9061 tctactacga tttcgacatt ccctcctatg agctgtgcga ggagatgtac cataagaccg
     9121 gtgcgttcgt cacgccgggc gactgctttg agcagccgaa gagcatgcgc atcggctacg
     9181 cctacggcaa ggaagcgctc gtgaaagggc tgaaggccgt gagcgagtac tttgcgtgga
     9241 aggaaaagga gctggaaaaa tgagcaccat ccgcgtcatc ggacgggagg atatccagaa
     9301 ggtattcact gtgatcgcgg cgctccgcgc cgtagagagc gcctacgccg ggaaatccgc
     9361 cggaacgggc cgcgtgtggc cgatggtatt ccatgagttt gaccccggcc acgccgatct
     9421 cgacatcaaa tccggcgatc tcgaaagcgc gggcgtatac ggtctgaagg tcgtttcatg
     9481 gtatgactcg aacgcggaaa aggcgctccc ggcgcttttc ggcacgtcgc tcctctttga
     9541 tcttgccacc ggcgagccga aggcgctttt gaacgccggg ccgatcacag cgctgcgcac
     9601 cggtgcggcg gccgccatcg gcgcaaaata ccttgcgcgc aaggacgcga aaacgctcac
     9661 gatcgtcggc tgcggcgagc tctgcccgta tcttgccgcg gccgcgctcg cggcgatccc
     9721 gttcctcaag accgtgtatc tcacaaaccc gcaccgcccc gaaaaggccg ccgagcggct
     9781 cgccgctgtg acagcggcaa cggacgcgct gctcaaggcg tgcggtcatg cccgtaccgc
     9841 cgcgatcaac gccgcggcag atgcggcatc cgccgtcgga gaaagcgaca tcgttttcac
     9901 cgccacctgc gccaaggcgc cggtcgtgaa aagcgcctgg gtcaagcccg gtacgcatgt
     9961 aagctgtatc ggcgccgacc tgcccggcaa gcaggaggtc gaatcggcgg tgctcgcccg
    10021 cgccgccgtc tacggcgacg atacggcgca gtgcttcgcc gtcggcgaat gcgagctgcc
    10081 gcacaaagag ggcgtcctcc ccgctctcgc cggggagctt ggcgaggtca ttctcggcga
    10141 aaaggccgga cggacgagcg atgcggagat caccgttttc gactccaccg gcatcgcact
    10201 gcaggacatc gcctccgccg cagtcattct caatgcctgc gaaaagcagg gactcggcac
    10261 ggtctgcgat atctgacgag agaacgatcc gtagcctcat cgcaggcacg cccnnnnnnn
    10321 nnngcttttg cccgctataa actgtataat aaaaaaaaga cacgaccccc ctgtcggaat
    10381 gttcgctttc catccctcgg gcatcgtgtc tttttgaaac ttttgaccgg caccgcaccg
    10441 tttcggttcc ggcacagaat ttgcagctgg gtgctatgaa caatgataca ctttgataaa
    10501 accaatattg aggtgacctc aaaccatact gtcacgctct cctgtgacac atgggtggtg
    10561 tttgaacatg atgattccga gaccgtcgct ttcggcacgc cggaatactg ggatgaggca
    10621 gttcaaacgg tttttggttc cggctacacc gtatgggaga ttgttgaaaa agcgctgccg
    10681 cgcagcgcgt gcaaccggat cacggatcac aaaaaggtat gggggcttct cgggctgccg
    10741 gagggcaaat tcgccgcgcg cgtggattgt gaagaaaaaa gcgtttatct tggtgcctca
    10801 tacggcctca aaacgctgaa agacagcaag gcatactctt atttcctgct ctccaacgca
    10861 gatgaggcaa gtgccgcgcg agtatgggat gacttctgcg gagaatacgc agcgcggcaa
    10921 tgcaatgatt ttattggcag cgccctcgct gtcgcggcaa aagagcggga ctttatcgcg
    10981 ctttactact cctatacaga taaagccgcg ctgaccatcg tgtgcactgc gcccgagcgt
    11041 atagccggtg agctccgctc catacccgtt cccgatggga tcgtgccgca ttaccgctgc
    11101 ttttggcggt aacgccggtt cgctaaaagc gaggcggcag gaatgtcgaa tcgtatttct
    11161 ttgcaatact ttttggagag gggatcgcaa acaatgagga caagaacctg ttaccgtctc
    11221 cgggatgtga gctggctcgt gcttttgcgg agcggcacgc agtcctctcc cgtgtccgcg
    11281 aagcgctgcg gtctggagct tccgacgcgc ttttacggca gggtgcggga tgattcgttt
    11341 cgcattcgtc cggtcggtat gcgcaccatg gcgtgtatct gcgggaaagt gacggaacgg
    11401 gacgaagaga aggttctgga ggtatgtctc cgcccgcgca gtctgatttg ggtgtttctg
    11461 acagtgtttt gccttcttac cgctcttttt gctttttccc tgttcgccgg gctgatcgcg
    11521 cgctcctggg gcgtagttct ggcatcggcg gccactctcg cggccatggt tttgctgtac
    11581 cgttttttcg cccagtcgga agttcgctcc ctagccgcgt actataagcc gatattcaaa
    11641 agctacggtc tggaggaaat cacgccatga gcatcaagga tatcaaaaag gatgaggttc
    11701 ggggcttcaa aatatccggt gacgccatat cggagcttct ctgggagctg ctgaacaaat
    11761 ccggcgacat gcttttggac cttcccgaca attcggatac gattttccac atgcactggg
    11821 accgcgaaaa agacgagctg acattttacg cgctggagtt ccgtgacccg catccggtcg
    11881 atttcaaagc tatcgaccgg tatatcgatg aaaccctcgg tatcacaacg gattccgttt
    11941 ttctgcccaa aggaaagcct taccgaagct tttctgtcag agaaacgcgc aaataaccat
    12001 gagcaaaatc cagcgtatta caaaaccctc gccccggacn nnnnnnnnnt tctgcaattg
    12061 caattattcg acgcggcctt cgaggacctt gagatcgtag ggctcgaatt tcacgtggcg
    12121 gtaggcttcc acatccagcg gcacgccggt ccagtcggag tgcggctcgc cggtctgctt
    12181 gacgagaacg tcgtagagaa tgtcatacgt cgtgccgtcg atcgtctcgg tcatcgtgct
    12241 gtagggcagc gtgcggtgat aggcgatgtg ctggccgtgg ttgttgaagt ggtagccgca
    12301 gcggatgcgg cagccgtcca gacccagata ctcaaagttc ttcataagat cggcgaggat
    12361 ctggtcgccc tctcgctcgt agaggttttc gggtgtggtg atggtgtagt ccgcgcggaa
    12421 ctggatgggc aggccgtact ttgcgaagcg gttggcaaaa gcgattatgc cgtcgtgcgg
    12481 atagttcttg aagagcacgc agttgatgcg caccggcacc ttgagcgagc cgagaagctc
    12541 gtcgttgcac tcctccacat agtgcaccat gtggcgggag atgttgatgc aggtgatctt
    12601 atccttgttg cgctccgtga acgcaatgat gcgctcggcg gggaaaagat cggataccgg
    12661 gagcgtcgta ttgatgaaca cacggtgacc ctccgggatg cagtccatca tcacctgcag
    12721 cgcgtccggc tcggaaaacg gctnnnnnnn nnncactttg gcgtgatctc atggaacatg
    12781 cggatgctct cgcacacctt ttccacgctg aagccttcgg gattctggta ttcctgctta
    12841 ttgatgcaga acgggcagtg gtttttgcag tcgtacggga cgaacaccgt gacggtcgcg
    12901 ccgccctcgc gggtcatata cgggttgtgg tttgtcatat tcttcaccta catagggtaa
    12961 tttcaaaaag gtattataac acagccttaa cagaaaagcc agaaaaaaac aacataaagt
    13021 tgaaaaaaac gaaaattttc cggttttact cgcttagtac gtcacgtccc agaggaaaag
    13081 cccctgcgcc ggggcggtaa aaccggcact cgcacggctg tgcgaagcaa ggatcgcttc
    13141 catatcgtcc ggacggcgct ctccgagacc gacctcgagc agcgtgccgg tcaggatgcg
    13201 caccatattg tataagaacc cgtcgccggt atacgtaagc cgcacttctt ccccgcagca
    13261 ttcgatcccg atagactcta tcgtgcgcgt ggcggcgcgg cccgttttct ttccggtgga
    13321 aaaggcagag aattcgtgct gcccgcaaag ctgtgccgcg gcgcggcgca tggcgtcaag
    13381 atccagcggg cgcggctccg catgcacata ctttctttca aatacgcagg gcgcatcgct
    13441 gttccagaca cggtaaatat acgttttccg caccgccgaa aggcgagcgt ggaaccgttc
    13501 cggcgctttc tcgagcgcga gcgcgccgat atcctccggc agatattccc ggagcagcgc
    13561 caaaagctcc gcgcaggact tgtccgtatc cgcccggaag gagcacacct gcattctcgc
    13621 gtggacgccg gcatccgtgc ggccgctgcc cgcgatctcg atgggctgct ccagcgcgcg
    13681 ggagagcagc gtctcaagct tgccctgcac cgtctgggcg gtgtttccct gcttctgcca
    13741 gccgcggtag cgcgtgccgt cgtagcaaag cgtcagtttg taattttgca tcgggcaact
    13801 cccttctttt tctcgcagag cttagtatag cacatttgaa tacatcgtcc atatactttt
    13861 cgatattttc tgtttttctg ttgaatctgc cgatccgttc aaatttgttt acattttccc
    13921 cggaatttgt tcatgaaatg ttcactcttt gatttttacg ccggttataa tagcatcata
    13981 cagagcatcc ggtgtgcagc cgccgaatga tctgaatgcc tccatcttaa tttaaaaaaa
    14041 tccgccgctt cttgcggcgg attttttttg cttttttcgg atagtctgga gcgaatctga
    14101 caaatacttg ttgcattgtt acacagcgct tggtatattt aaataaacac catgcggatc
    14161 cccttcctgt gcagcgtccc tcactccatt cggaaagcgg cggtctgacc gcttttaaaa
    14221 taaagaagag aattgggagg acatcacatg aaaaagattc tcgctgttat cctcgcgctg
    14281 gccatggttc tggctcttgc cgcctgcggc gcggcgccca ccacagcaga acccactcca
    14341 gaagcgcccg acccgctcaa atccgaaatg ccctcgaaga tctacgagga atccgccgca
    14401 gccaacccgg agggaaaaac cgtgaacatt cttcttgtca tcgacatgca gaaggatttc
    14461 gtcgatcagg cgctcggcac cgccgaggca caggccatcg tcccgaacgt tgtggccaag
    14521 atcaacgaat acaaagagcg tggcgacgtc atcatcgcca cgaaggacac gcacgaggaa
    14581 tcttatctcg aaacgcagga gggcgtcaac ctcccgttca tccactgcat gcagaaacct
    14641 ccgcaggatc tccagccgct gcaggcggga taccgagggc tggcagctgg acgacgcggt
    14701 gcaggccgcc atgccggaga acgccacgat cgtcaataag ccgaccttcg gctcgaccga
    14761 ccttgtggag atcatcggcg aatacgtcgc gcagtacggc gagccgaacg tccatgtgga
    14821 gatcatcggt ctttgcaccg acatatgcgt tatctccaac gccctgctcg aaaaggcgtt
    14881 ctatcccgaa atgcccatca cgctcgacgc ctcctgctgc gcaggcgtca ctcccgccaa
    14941 gcacgacgcc gccattgaga ccatgaagag ctgccagatc ggcgtatata acgacaccat
    15001 taccgcgccg ctctccacgg aggttctcaa agcgccgaag gcctccgaga tctacgccca
    15061 cgccgaaaaa gtcaaccccg agggcaagac cgtcaacgtc ctgctcgtca tcgacatgca
    15121 gaaggacttc gtcgatcagg cgctcggcac cgccgaggcg caggccatcg tccccgccgt
    15181 tgtcgccaag atcaacgaat ataaggcccg cggtgacgtc atcatcgcca cgcgcgacac
    15241 gcacgaggac tcctacatgg atacgcagga aggaaagaac ctgccggtca tccactgcgt
    15301 agaggagacg gacggcttct caacaccgag gtcgccgacg cgctcggcga atacgccacc
    15361 gtgatcaaca agcccacctt cggctcgacc gatctcgtag cgctcatgcg cgagtatgtg
    15421 cagcagtacg gtcagccgaa cgtcaatgtg gagattattg gtctctgcac cgacatctgc
    15481 gtcgtatcga acgcgctgat agaaaaggcg ttctatcccg aaatgcctat cacgctggac
    15541 gcttcctgct gcgcgggcgt cacccccgca acgcacgacg ccgcgctcgc cacgatgcgc
    15601 atgtgccaga tcagcgtcat caacgctgac tgatttcttt tctgacatac acatcgcccc
    15661 gccgaacggc ggggcaacag actgaaatca cccgttggga gttttcccaa cgggtgattt
    15721 gagcattggt tatcagtttt tggagaactc cgcgagctcc aggccggagt tctgcttgag
    15781 cgtgtaggcg atgttccact cgccgccgaa gagcaggagg ttgttgccct tgaagtcctg
    15841 cacccacttg gtgtcgcgcg tgagcgtgag cgttgcgggg tcggtgtcgc taacgatcca
    15901 gcggatgttt tcatacggca gcgcgcggcg gcgcacggcg gtggaatatt cgctctgcaa
    15961 acggtattcg agcacgtcga actgcagcac gccgacgacg ccgacgatga cctcctccaa
    16021 gccggtgttc ggctcgtgga agatctggat ggcgccctcc tgcgcgatct ggcggatacc
    16081 ggtctcgaac tgcttgcgct tcatcgtgtc cttgcgctca aaggcggcga agtgctcggg
    16141 cgcaaacgtc gggatgcctt caaagcggac ctttttgtcc ttggagcaga tcgtatcgcc
    16201 gatggagtag atgccggggt cgaacacgcc gatgatgtct ccggcgtaag cctcgttgat
    16261 gatctggcgc tcctcggcca tcatctgctg cggctgcgcg agcttgatgg gctttccgcc
    16321 ctgcacgtgg tagtattcct cgttgcgctc aaacttgccc gagcagatgc gcatgaacgc
    16381 gatgcggtcg cggtgcgcct tgttcatgtt cgcctggatc ttgaatacga aggcggagaa
    16441 atggtcgtcc gtcggctcga ccggcacgcc atcggaaagg nnnnnnnnnn aaggcgcgcg
    16501 agcggcggcg ttgtgagatc gagaaagctc gccagaaact gctccacgcc gaagttcatc
    16561 agcgccgagc cgaagaaaac ggggctcaga tcgccgctgc ggacaaggtc gagatcaaat
    16621 tcgtaccccg cgccgtcgag aagctccaca tcgtcgcaaa gcgtgctgta cagccgctcg
    16681 ccgagcgctt cgcgcaggcc tgcctcgtta tccatggaaa gctccgccgc gtggatggcg
    16741 cggctgcccg cgtgacgctg cgtatacgcg agcacttcct tcttctcgcg gtcgtacacg
    16801 cccttgaagt ccacgccgca gccgatcggc cagttcatcg ggtacgtgcc gatgccgaac
    16861 tccttttcga gctcttcgag cagatcgtag gggctgcgcg cctcgcggtc catcttgttg
    16921 atgaacgtga agatggggat gtgccgcatg gcgcagacct tgaagagctt gcgcgtctgc
    16981 ggctcgatgc ccttggccgc gtcgatgacc atgacggcgc tgtccgccgc catgagcgtg
    17041 cggtaggtgt cctcggagaa gtcctggtgg ccgggggtgt cgagaatgtt gatgcagtag
    17101 ccgttatatt caaactggag cacggacgac gtgatggaaa tgccgcgctg cttttccagc
    17161 tccatccagt cggagaccgc atggcgcgcc gccgccttgc cctttaccga gccggcgagc
    17221 tggatcgccc ctccgtagag aaggagcttt tccgtcaatg tcgttttgcc tgcgtccggg
    17281 tgggagatga tcgcaaacgt ccgccgccgc gagatctccc gctgtaagtc actcatgttg
    17341 tttcatcctt ccggtaaatt tttgaaatga aaaatctacc gtatttttaa tccttcataa
    17401 cccgtctctt cccttgaaaa agttgtaaca ctctttttta ccatagaatt cccgaaaaag
    17461 caatgcgcgc attccttctt tgcggggaaa ttgaaaaaaa gcccttgcct ctcccattga
    17521 gaaatgcacc ccattcctta gtactaagta ttttacgata cttgtctaac gaatggggtg
    17581 ctgttcactg tgcgggggag gcttcttttt ttcttattgc cggagctcag tatacggcgt
    17641 atagtcgagc gccgtatagc gttccgtgtg ggaatcgatg attgcctgac aggccgcctc
    17701 atcggacggc gtgtcattgg aaacgtcgta gtccgtatcg tacgtcatgt accacgcgcc
    17761 ggactcgctc gtcacgccct gcataagagc aagctctccg ttctggagcg catagtaaaa
    17821 ccacacactg ctcgccgcgc cgcccgaacc ctcgttcgca atgagccagg cgttttcgtc
    17881 cgcgagatat tccacataat acttatctct cgcgtggctc tgcgcgagca tcaccggcgc
    17941 gccgtccttg accgtccaga tctccagaac ggccggatcg gtgtccgcgc ccaagatcgc
    18001 gccgatcacc atctccggcg agccgtcgcc gtcaaggtca gggaaaaata cgccgacgtt
    18061 cgcgcgggcg tcgccctcgt agtagtaccc cgcgagctcg ctcataccgt tttcaaaata
    18121 ccgtccctcc ggccactgct cggagagcgc cgcggcgtag cggtccagct gcgcggcata
    18181 gagcgttttc ccctcttcgg ccatgttcac gatgccggtc gatttctcca ccggctggga
    18241 gaccggctcc gcggtggcgg tcggttcagg cgtcgatgtg ggcactgctt cggtgggctg
    18301 cgcgccgcag gctgtcaaga gcagacttgc cgccagcacc aaaataccga tccatttcat
    18361 cttcttgttc attgtataac ctcctgcggc cgggttttcc gctccgcagg atattagacg
    18421 cgctatgcgt caggatggtt cacacggagt ggataaattt ggttcctgcc gcgcctccca
    18481 cgcccaggcg cgctcgtcca cggcggtttt cccttcgcgc agcgccgcga tctcccgccg
    18541 gcagagcggc acagcggcgg tcagaaattc ctccgcaatg gctttggtga aatgcacgca
    18601 ctcgcccggc gggggcggcg tcgtgaaatc tgcatgcagc tcggcgagaa attccggcag
    18661 cgcaaaatcg gaaacagcgc agatcggctc agcggaaaaa tcctccggcg cggtaaggtc
    18721 ctcccgcagg gcgaatttct caatggccca ggtattcaga atgcggtaat cgtcgtaaag
    18781 ccgctcgacc ttctgcggcg agtccacctt ccagccgtgg tcgagataga ataccttgcg
    18841 gaacacgatg tcctgcacaa gatggagata gtacccgaga tagagcggat cgtccatttt
    18901 gtccgcgaag cgcgcgcgga agccgccgag cgccatcatc gtcttcgttc cgccgtcgat
    18961 ccgcacgcgg aaatgcgcgc tcacacgctc tccggcgtcc ggtaagatcg cgccgagacg
    19021 aaagcgcttt tcgtccgccg cgggatgctc gtccagaatg agctttgccg cggcgagatg
    19081 aaggatacgg gatgccatgg tctttcctcc tgtatacgaa aaagacggga cgctgc
//
LOCUS       DAWAAE010000021        18977 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1303_length_18977_cov_3.357679, whole genome shotgun sequence.
ACCESSION   DAWAAE010000021 DAWAAE010000000
VERSION     DAWAAE010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 18977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18977
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_18977_cov_3.357679"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    402..411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1903..1912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10847..10856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15742..15751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3538 a         5894 c         6085 g         3420 t
ORIGIN      
        1 agaccccagc ggcgctggcg ggagatgcac cagtcggcgc gctcgcggat catggagagc
       61 atgcggtcgt ggccccagcc gggatgccag atgaccttgt ccgcggcggc gcaggcgtcg
      121 tccttgaacg tatcgaccga gcagaaccac tgcggcgtgg cgcggaagat gatgggcttg
      181 tggcagcgcc agcagtgcgg gtagctgtgg acgatatcct cgctcgcaaa gagcatgccg
      241 ctctcgcgca tatcctcgag aatgaccggg ttggattcct cggtcttcat gccggcgtat
      301 ttgccggcgt agtcggtgtg gcggccctgg tcgtccacgg ggacgatcat gtccatgccg
      361 tagcggcggc aggtctgata gtcgtccgcg ccgaagccgg gnnnnnnnnn nggtgtggac
      421 gcagcccgtg ccggagtcca tcgtgacata ctcggcgttg agcaggcggg acgtcttcgg
      481 caggaacggg tgatcggcga gcatgttctc aaagaacgag ccggggtgcg tttcgagaat
      541 ttcgtacttc tcaatgccgc cgacgcgcat gaccttctcg gcgagcgctt cggcaacgat
      601 gtagatctcg ccgttatcgg ccttcacaag cgcatagctc tctctcggat gcaggctgat
      661 gccgaggttg cccggcagcg tccagatcgt cgttgtccag atgaggaaat agagcttgct
      721 gtggtcgacg tgggagagct ttccgagatc gtcatgcatc gggaacttga cgtacaccgt
      781 ggtgcaggga tcgtcctgat actcgatctc cgcctcggcg agagcggtct cgtcgctcgg
      841 gcaccagtac acgggcttga gacccttgta gatgtagccc ttttcgtaca tcttcccgaa
      901 caccttgacc tcttcggcct caaacttcgg gtccatggtg agatacggat gatcccagtc
      961 gcccagcacg ccgagacgct ggaagccctc gcgctggatg ccgacgtact tttcggcata
     1021 gcggtggcag gcggagcgga actcgctcac gctcatggcc ttgcggttga gccgctgctc
     1081 cttgatgatg gcggactcga tgggcatgcc gtggttgtcc cagccggggg tataggggac
     1141 cttatagccg gtcatggatt tatagcggca gatgaagtcc ttcaggcact tgttcagcgc
     1201 gtggcccatg tgcagattgc cgttcgagaa cggagggccg tcgtgcagaa tgaagagggg
     1261 cttgccctcg ttgcggcgga gggtctcgtg gtaaagatcc atgtcgtacc aggatttgag
     1321 catgcccggc tcacgcgcgg gcagaccggc acgcatgggg aaagcggttt tcggcagatt
     1381 tacggtggca ttgtagtcca tagctgtgca attctctcca ttcgctgaca gagcggggcc
     1441 gatcgagccc cgccctgtgg gttttttctt taaaaatcag aatttgatct ccggggcgtc
     1501 cgtgtcggaa tcctcgggct tctgatagac agcgttttca aacgccttga cgtagtcctg
     1561 ctcgtcctca gcgtccgggt agaggggaga ctcctcctct tccgttttcg gctcggcttc
     1621 gggttcggct tccggctcgg cggcggggag gacttcctcc tcaacggtct cgctctcggc
     1681 cgcttccgcg ggggcggcga tggccttctt ctcggcgggc gcaggcgtca cgacggcgcc
     1741 ggtgaggtcg gcctcggcga gcgtggtaag gaaggtctgc tcgcggtcga gcagcatgcg
     1801 gaccttctgg atgtattccg cggcagcggc gcgcgccttc tcgcagcgca gctcctcggc
     1861 cttgcggttg gcctcgatcg tgccgatggt gcggtcagac tcnnnnnnnn nngactcggc
     1921 cttggcggcg gcgaggatat cgtcggcttc cttctgcgcg gaggcgccga gggcttccgc
     1981 cttggcgcgg gcgtcctctt cgatgttctt ggcgagcttc tgcgcggaga cgagcgccat
     2041 gttcatggcc tcctccgagc cctgatactc gcggatcttg tcggcaaggt atttcatctt
     2101 cgcgttgagc gtggcgatct ccttctggga ggcagcggcg tcattggcga gctcgtcgag
     2161 aaaatcatcg acctcgtcca tggcgtaccc gccgatgcgg gccttctcaa aacatttttc
     2221 gcggatgtcc tgggcagtga tcatatcttt ctactccttt ccggattctg ttttctatcc
     2281 gtcagcgccg tgaggcgcgg gaacgcttga ttaattaaaa gtacttttcg cgggattcga
     2341 cggcgttgcc ggtgtcgccc atgaagtcca tccccgcggg ggtgatgatg taggtgctgc
     2401 cggagacctt cttgaccttt ccgccgagcg cataggtaac gcccgagaga aagtcgagca
     2461 tgcggcgggt cagttccttc ggcgcggtct ccagattgag cacgatcgcg ttgcgctcgg
     2521 caatgcggtc ggcaatgtcc gcggcctgct caaagcggtc ggggtgcacg agcagcagct
     2581 tcggctgcgc gccgccgctc tgcggacggg cgggcgcggc atacccctgc tgggcatagg
     2641 gatcgggcgc atagccgctg cgggcatagg ggtcgggcgc gtagccctgc tgggcatacc
     2701 cctgctgggc gtagccgtaa tcctgctgcg cgtaggggtc ggcggcctgc tgctgccgcg
     2761 cggcgcgctg gttggagaat acatacggcc ggcgggtcgg ctgcacgggc tcggcgctgc
     2821 gcggcgcttc ggcgtaggac gcgtaggagc gcgcgtccgc cgcgcggccc ggcgcgcgta
     2881 ccggctcggc gagatcttcc tcgagataat cctcgtcgtc gtagggtttc gtcagttttt
     2941 tcagctcatc caatagtccc atacgggggc gcctcctgca aatcgtaatt ccgggggccg
     3001 aacagcgccg ttcccagacg gacgatggtc gcgccgcagc gcacggcttc ctcgtagtca
     3061 ccgctcatac ccatcgacag ctcagccatt gttacattat cgtatgtttt ccggcggatg
     3121 tcaacaaaaa gctcgtacat ttgtcggaaa taccaacgat tttcttctcc ggcgctcacg
     3181 gcgggcggga tcgtcatcaa tcctttcacg cgaaggccgg ggcagagcgc ggcgtgggcg
     3241 aggagcgcct cgagcccgtc gggcgaaacg ccgcttttgg acgcctcgcc gccgatgttc
     3301 acctccagca gcacatcctg cacgatgccg agcgagacgg cgcggcgcga gatcgcgtcc
     3361 atcagctccg gagaatcgac cgagtggatc agctccaccg cgccgacgag gtatttcacc
     3421 ttgtttttct gcaaatgccc tatgaaatgg acgcagcagg tggcataggc gttcacagca
     3481 tttttggaaa gaaactcctg cacgcggttt tccgcgcaga tgtccacgcc ggcgcggatc
     3541 gcctcctgca cggcctccgg gccgttcatc ttcgttgcgg cgagaaggcg cacctcgctg
     3601 ccggagcggc cggcgctctc ggcggcggcg ttgatcttct gccgggccat gcgcacattt
     3661 tcggtgatgc tcatatcttt ctgtcctccc gaaacgtatt taaaaggaaa aagggtactc
     3721 tacttttaag atggcgcgct gtccgccgcg gggaaaagca gcacgctcat gccgccgcgc
     3781 agcatcggtg atgcgatgag cgccgcgccg ccgcgccgcg cgagcaccgt gaccgggacg
     3841 tattcccgca ccgggccgga cacacggcag acggtctcgc cgtcctcacc cggcagaatg
     3901 gccgactccg gcaccgaata gccctcccat tcggccagaa gcaccgcaag cgtgcattcg
     3961 ttcagggaaa gaacgctttc gaggtgcgtg ccgcaggaga aaacggcttc cccggcgtcc
     4021 gttatgcaca ctgcggcggt gacgcgcgtt tcgtccggca gaagaagcac ggcgctgccg
     4081 ccgtccggca gacgcgccgc ggtcttttcg ttgagcggcg cggtgaacgt ccagccgctg
     4141 ccggagacga cggtcatcgt gccgtccgcc ccgtgcgcgg agaaatatcc cgcgcgcggg
     4201 gcacggacga gcgtctcttc cgctccggag gaaaggagcg cttcctcctc ctgccggaga
     4261 gcgttgccgc ccgtcgcggc atagccgagc gaacggcgcg cctccggcaa ggctgcgccg
     4321 agcgcggaaa agtcccgccg ggcgagcgct tcggaaagcg agcgcagagc ctgtccgccc
     4381 ggcacgggca gcgtatcgga ggcaaactcc gcgcgcacgc gcagaaggag cgcggcgcgg
     4441 aaaagctccg ccccggtctt ccccgcgagc gcgaccggcg cccccgccgc gacgcgctct
     4501 ccgtccggaa cgagcgggta caccgcgcct tccggcgcgg aaacgggcgt ttcaccgcgg
     4561 gaaacgacgc ccttgagcga aagcgtatcc tgccagagcg cggcggtgca caccgccgag
     4621 ggcacatcct ccgcgcggga ggtgaaaaag agcagggcat atgccgccgc cgcgagcagc
     4681 agaacgatgc cggggaacag cagcggcagc tccgagcgct tcacgcgctc ttatgtattc
     4741 gtgccgccgg tcatatcgac cgactgcgac gggacgagcg tggagatcgc gtgcttataa
     4801 atgagctgct gctttccgtc ggtctcgagc acgaccgtga agctgtcaaa tccgcgcacg
     4861 atgccgcgca tctgaaagcc gttcatgaga aagagcgtca cgctctgtct gtctttgcgg
     4921 gtctggttga ggaaaaggtc ctgcagattc tgtgtttttt gcatgataaa agctcccttt
     4981 cgccggggtc tccggctttg tgataagtat tcacacgctt gggcgtgtgt tctatcattg
     5041 taaagagagc cgattaaaaa gccggtcgaa gcgtgtcaga ccgtcttccg gcggttgcgc
     5101 taatttttaa ggacggcggc gagaagatcg tcctccgtcg ggtattcctc ccgcggcagc
     5161 cagacgagat ccctgtcgcg ccgcagccag gtgagctggc gcttggcata gcggcaggat
     5221 tcgcgcttga cggtctcgat ggcgtcctcc ggcgagcact cgccccggag cgccgcggcg
     5281 atctccttgt agccgatggc ctgcatcgcg gtgcaggaat ccgaaagccc ggcgccgagc
     5341 agacgctcca cttcttcaaa aagccccgcg gcgaccatct cgtccacgcg ctgccggatg
     5401 cgcgcataga gcgtttcccg ctcgttccac gtgagcgcaa agcggcagga agcataccgc
     5461 ggcgggagcg cctttgtacg ggcgtcgtgc gcggtgatcg tctcgccggt gagcgtatat
     5521 acctccatcg cgcgcacgag ccgcttcttg tcgcccggcg cgagcttttc cgcccgctcg
     5581 ggatcgacca gccgcagctt ttcccggaac gccgcgccgc cgagcgcgtc atactccgcg
     5641 ccgagcgctt cgcgcacgcc ggaatcgccg ggcgcggcgg aaaaatcccg tccggagaga
     5701 agagactcga tataaagccc cgtcccgcca acgatgacgg gcgttttgcc cccggcaagc
     5761 agcgcgtcac agcacgccgc cgcgttttcg acccagcggc tgacgctgta gtcctcggcg
     5821 gggtcagcca cgtcgatcat atggtgcggc acgccgcgca tttcttcccg cgtgggcttg
     5881 gcagagccga tgtccatgcc gcggtagagc tgcatcgagt ccgcgctcac aacttcggtg
     5941 ccgagagcga gcgcaagact cacgccgagc gacgttttgc ccgaagccgt gggccctgtg
     6001 atgacaatga ctgtacgatt gttcatagac aaataattac acctctgcta taatccttag
     6061 catcgtgtta actctatgtg aactattttg gaaagggaga ccgtaaatgc caatgaagat
     6121 ccgaatttct gccgacagca cctgcgatct tacgagcgag ctgatcgagc agtacgacgt
     6181 aaagatcgcg ccgctctata tcctcatgga cgaccgcgcg ctgcgcgacg ggatcgagtg
     6241 cacgcgcgag gacattttcg cgtatacgag ccgcacggga aagctctgcg gcacggcggc
     6301 ggtgtccatt gccgactatc tcgatttctt cggcgaggag ctccgaaccc acgaccagat
     6361 cgtgcatttc acgatctcct cggatatgtc cgcctgcttc cagaacgcgt gcgatgccgc
     6421 agaggaattt ccggggcgcg tgttcgttgt cgattcgcgc aatctctcca ccggcatcgg
     6481 ccatctcgtg atcgacggcg cgctgctcgc ccgcgagggc aagtccggcg aggagatcca
     6541 ggcgatcctt gacgagcgcc gggagaagct gaacgtcagc ttcgtaatcg acacgctcga
     6601 atatctgcgc aagggcggcc ggtgctcgac cgtcgcggcg ctgggcgcga atcttttgaa
     6661 gctcaagccc tgcatcgagg tgcacgaggg caagatgagc gttgcgaaaa agtaccgcgg
     6721 cacgctcgcc aaggcgatcg agagctatgt gaccgaccgc atcaaggatc acgacgattt
     6781 cgacaagaag cgcgtgttta tcaccgactc cggcgtggat acggagatcg tggagactgt
     6841 cgaaaagctc ctgcgcgagt acggcggctt tgaggagatc caccacacgc tcgccggcag
     6901 caccatctcc ggccactgcg ggccggggtg ccttggaata ctctattata ataaataatg
     6961 ctttattaag cctgcctcct tcgagggagg cttttttatt gccccgtttc cggcagcaga
     7021 gccttccttt tccggaagta atacaccgcg ccggtgatat cggcgagcac ccacccgatg
     7081 gggatctcgg accagatgcc ggtgacgccg agcgaccctg ccgtgagata tgcgagcgcg
     7141 acgcgcgtgc cgagcgaaac gaccgtcagc acaacggaca tcgcgggctt tttcaccgcg
     7201 cggtagagcg cgtagaagag gaacagaaac ccgatgccga agtagaacgg cgcgacgcac
     7261 cggagatagt aaacgccctc acggatgacc tccgtgtcgg ccgcggggat gaagaggcgc
     7321 atgagcggcg aagcgaacag cagcaccgcc gcggaaatga ccgcgcagaa gccgagcacg
     7381 agtgcaaacg agcgccggat cccggcgcgg atgcgatcct gctgccgcgc gccaaagttc
     7441 tgcgcgacaa acgtggaaaa ggcgttgctg aaatcctgcg ccggcatata cgcgaatgat
     7501 tcgatcttca ccgccgcggc gaacgccgcc atgacgatct ccccgaacga gttcacgagc
     7561 ccctggatca tcagtatgcc gaaattcatc accgactgct gcacgcaggt gagcgaggag
     7621 agcgagaaga tctcgcgcag aagcgccgcg tcaaagcgca gctcttccct cttcggcagc
     7681 agatcggggc agcgccgcag cgtgaaaacg gcaagtccca gcccggagaa gtactgcgcg
     7741 atgcccgtcg cccacgccgc gcccgccaca ccccacggaa aaacgagcac gaacagcaga
     7801 tcgagcgcca cgttgccgag cgccgaaatg ccgaaaaaga cgagcggggt catgctgttg
     7861 ccgaccgagc gcaggagaca ggcaaaaaag ttataaagga acgtcgccgg gataccggcg
     7921 aagatcacgg caaggtacgt ccgcatgagc ggggaaacgc tctccggcac ctgcaaaagc
     7981 cgcagaaggg cgggcaggag cgcataggcg agtgccgtga ggaggagcgc acatacgccg
     8041 atgagaccga acgcgtggac gatgctctgc cagagaccgc tctcgtcgcc gcgcccgcgg
     8101 cagatggaga aaagcgcgcc cgcgcccatc gaaagcccta gcagtatcga cgtgaggaag
     8161 atcataagcg tatacgcgct ccccacggcg gcgagcgcgt cacgcccaat gacgcgcgag
     8221 acgatgagtg tgtcggcaat attatagagc tgctgcaaaa tatccccggc gagcatcggc
     8281 agcgcaaaga gaagaagcga gcggagaatg cttccctttg tcagatcgcg gtacacgtca
     8341 cacgatcctc ccgaagcgct tgtcgagctc ggcgcgggtg aataccgtca tcaccgggcg
     8401 gccgtgcggg caatgtgtca cctctccggc gagcaccttt tccgcgagag ccaaaagctc
     8461 ctcccgctcc gtgacccagc cgcccttgat cgcggccttg cacgcgaccg tggcggcaag
     8521 acgctcccac acgtccgagg acgagagcga ccggccctcc cgcagctttt ccaccagctc
     8581 ttcgagcagc ggcacggcgg aggcggcgtc gagctgcgcc ggtacggcgc ggatgaggaa
     8641 tgtcgtatcg ccgaaggcgt ccagctcaaa gccgagccgc gcgagaagcg cggcgttcgc
     8701 ctccaaaagc gcggcgccgt ccgggtcggt gttgaacggc tggggctcca gaagctcctg
     8761 cggcatgagg ggcttttcgt tcaggcgcat tttgtcaaag agcaggcgct cgtgcgcggc
     8821 gtgcttgtcg atgagcagca tcttgtcgcc ctgctcgagc agaatgtaaa cgttcagcgc
     8881 ctcgccgatg tagcgcacgg gctcggcgcg gaaggtctcg tccagcaccg tctgctccgg
     8941 cacggtctcc gccgccgttt ccggggcggg aaggctcttt tcgaccggcg cggcgggctc
     9001 cgccggggag accggcgctt ccggcgcgga gacgggggag cgcggcgcgc tcacggacgg
     9061 aatgtccggc gtataggtgc gcaccggtac ggagaccgtc gcggaagaga ccggtttcgg
     9121 cgcggcgggg gaagcggcgc ggtagggcgc gctgtcgcgc acgccggatg tgcccgccgg
     9181 acgggcgggc gcggcggagt atgtctgccg gaacgtgccc gcgtccatcg tgcggaagaa
     9241 gtccttgcgc ggcgcgctcg cggcggccgg agcgggacgg gccggggaag aaacgggcgc
     9301 ggcgctcttc gcttccgggg caaaaggcgc gttcgacatc gtctgcggcg cgctcttctg
     9361 gccgagcgcg gcgagcgtgc cgtagtaaac gccgtcaaaa acgggcttct cttttgcgaa
     9421 ttttacctcc gccttcgtcg gatggacgtt cacgtccaca agaccgttgg gcagcgtgat
     9481 atagagcacg cacccgggga acttgccgac catggcggta tttttgtatg cctgctccag
     9541 cgcggcctgc aaagcgcgcg agcggatggg gcggccgttg cagaaaaagt actgcgcgcc
     9601 gcggttgccg cggcagtaac cgggcgcgga aacgaagccc tttaccgtga cgccgtcgct
     9661 ttcggtctcc accggcagca gtcccgccgc ggcgtcgcgc ccaagcagtg cgtatacggc
     9721 gctcttcgcc gtgccgtcgc cgggggagaa aaattcctcc tgcccgtcgc gcaggcagcg
     9781 aaagctcacg tccgggcggc cgagcgccgt gcgcagcgcg ctctgcacgc acgcggcgct
     9841 ctcgctccgg tcggtgccga gaaacttctg ccgcgcgggg gtgttgtaga aaagatcgcg
     9901 cacggtgatc gtcgtaccct ccgggcagcc ggtctcctcc atggagacga tctccccggc
     9961 ctccacctcc acgcgcacgc ccatggccgc gccgcggcgg cgcgtcgtga gcgtgatgcg
    10021 cgaaacggcg ctgatcgccg cgagagcttc gccgcgaaag ccgagcgtgc cgatggcctc
    10081 gagcccgcgc tcgtccgaga gcttgctcgt cgcgtggcgc agaaagcaca cgcccgcgtc
    10141 ctccggcgcc atgccgacgc cgtcgtccgt gacgcggata tagtcccgcc ctccggcgcg
    10201 gagctcaagc acgacggtgc gcgctccggc gtccaccgcg ttttccaaaa gctccttgac
    10261 cgccgaggcg gggcgctcaa cgacctcgcc cgcggcgata aggtcggcga cgtgcggcga
    10321 aaggacgtgg attaccggca tgatatatgt acctccagaa tgggaatcga taatatttca
    10381 ttctgattat aaagcaaagc cgttgcggaa tccagagcaa aatgttaaaa tactctatta
    10441 tcggaagaaa agcctccctt gtgtaaaggg aggtggcacg gcgaagccgt gacggaggga
    10501 ttgtcgtttt ccgaaagcta caatccccca gtcagcctcc ggctgacagc cccctttgca
    10561 caagggggcc gttcacaagg atacacggaa aggaaataga tttatgccct acgagagaac
    10621 cgaggtctcc gcgcaggaga tcgaacggca gaaagagata tgcgcgcgcg tccgctccgc
    10681 cctcggcagc ggcgagcgct tcgccatggt agacacctac ggctgccagc agaacgagtc
    10741 cgacagcgag gtgctgcgcg gttatctccg cagcatgggc tatacgatga ccgacgacga
    10801 ggacaaggcg gacgtcattg ccgtgaacac ctgcgccgtg cgtgagnnnn nnnnnnatgc
    10861 gcgtgttcgg caacgtcggc gcgctcacgc atttaaagcg cgtccacccg gaacgcatcg
    10921 tgattctctg cggctgcatg gcgcagagcg aggagatccg cgagcaggtg aaaaagtcgt
    10981 accgcatggt cgatctctgc ttcggcccgc acgagctatg gcgcttcccg gagctttttg
    11041 agagaacgct gcaggtccac ggtctctccg gcgcgaaggg ccgcgtgttc gatgccgaga
    11101 gcaccgaatc cattgccgag ggtctccccc gcgagcggga aggcagcgtg aaggcgtggc
    11161 tctccatcat gaacgggtgc aacaacttct gctcatactg catcgtcccc tacgtccgcg
    11221 ggcgcgagcg ctcccgccgc agcgaggacg tcgtgcgcga ggccgaagcg ctcgtcgccg
    11281 ccgggtataa ggatattacg cttctcggcc agaacgtgaa ctcctatggc cgtggactgg
    11341 acgaggacgt cgatttcgcg gagcttctgc gcaaggtcaa cgccattccg ggcgatttcc
    11401 gcatccggtt tatgacgagc cacccgaagg acgcgaccga gcggcttttt gctgccatgg
    11461 cggagtgcga aaagtgcgcc ccgcacatcc atctgcccgt gcagtccggc agcgaccgga
    11521 tcctgcgcgc catgaaccgc ggctatacgg cggaaaagta tctcgcgcag gtcgatcttg
    11581 cgcggaaata catcccggac ctcgtcctca cgaccgatgt gatcgtcggc ttcccgggcg
    11641 agacggagga ggacttcctc gatacgctcg cgcttacgga aaaggtgcgc tttgactcga
    11701 tgttcacgtt catctattcc cgccgtccgg gtaccccggc agcaaagatc gaggacgata
    11761 cgccgcgctc cgtcattcaa aaccggttcg accggctcgt ggagagcgcg ggacgcgtct
    11821 ccgccgagct gcaccgcgcg caggaaggaa agaccttccg cgtgctcatc gacggcacgg
    11881 cgaaaaacgg cgactatacg ctctcggcgc gcaccgccgg gggacggctc gtgcatctgc
    11941 acggggacga gtcgctcatc ggctcgtggg ccaacgcccg catcgtttcg agcaatacgt
    12001 tcgcgctctt cggcgagatc acggagtaaa ggggagaaag acattgcccg cagaaaccac
    12061 ccccatgaaa atgcagtata acgccatcaa ggccgagcat cccgactgcc tgctctttta
    12121 ccggctgggc gacttttacg agatgttcga cgatgacgcc cttctcggcg cgcgcgagct
    12181 gaatcttgcg ctcacgacgc gcgaccgcgg caagcccgag gacgagcgca cgccgatgtg
    12241 cggcgtgccg taccacagcg cggagcagta catctccaag ctcatcgcca agggctacaa
    12301 ggtcgccgtg tgcgagcaga tggaggaccc gaagctctgc aaggggctcg tatcgcgcga
    12361 tatcgtgcgc atcatctccc ccggcacgat catggagacc tccatgctgc gcgagggcga
    12421 gtcgaactat ctcggcgcgg tcatgctgcg cggcaaggca ggcgggtgcg cctttgcgga
    12481 cgtgtccacc ggcgaggtgt gcgcggcgtc gtttgaaacg gacgcggcga tgcacatcca
    12541 gaacgaaatt tcccgcttcc gaccacgcga gtgcgtgctc gcgccggagg cgatgctcag
    12601 ccgcgacatc cgcgacacgc tcgaaaagcg gctcggcagc cgcgtggagc ccgccgcggg
    12661 gcggttcgat cccgaaaacg cgcgcaaagc cattgcaaat cagtacggcg aaaacgcccc
    12721 cgagctcgac gagatgacga cactcgcgct cggcgcgctg ctcgcctatc tcacggacgc
    12781 ggcgcgcggc gacatgtcgc agctgcgcgt gccggatatc ttcacgcggg gccggtatat
    12841 ggagcttgac gccgcggcgc ggcgcaatct cgagctgacg gaaaacctgc gcaccggcga
    12901 aaagcgcggc agccttctct gggtgctgga taagaccaag accccgatgg gcgggcgcat
    12961 gctccgcagc tgggtggagc gccctctgct ctccccggtg gcgatccggc ggcggctcgc
    13021 cgcggtggag gagctcttcc gcgataacgt cggccgcgcc gagctcaccg aaaagctcaa
    13081 gaccgtcggc gatatccagc ggctcacgtc ccgcgccgtg tacggcgcgg cgaacggccg
    13141 cgacatgctc gcgctcggga actatctctc cgccgcgccg gagatcttcg cgcagctcgg
    13201 caagtaccag agcgggatgc tccgctccat cgccgcaacg gagccgctgg aaaacctctg
    13261 cacgcttttg cgccgcgcca tctgcgatga gccgccgttc tccgtgcgcg agggcggcat
    13321 cctccgctcc ggctacagcg aggaggtaga ccgtctgcgc aacatccgcg acaacggcgc
    13381 gaagtgcgtc gccgagctgg aggcgcggga aaaggagcgc accggcatca agaagctcaa
    13441 ggtcggctac aacaaggtgt tcgggtatta tatcgacgtg ccgaactccg ccggagccgt
    13501 ggagctgccg gaggaataca tacgcaagca gacgctcgtc aacggcgagc ggtatttcac
    13561 ccccgaacac aaaaagctgg aggacgatat ctcgtccgcc cgcgagcgga tcgaaacgct
    13621 cgaatacgat ctcttccgcg acgtgctccg gcaggtgggc gaccgcgtgg acgagctgca
    13681 gacgctctcc gcggcgctcg cggagctcga tgcgctctgc tcgcttgccg aggtcgcggt
    13741 gcgcaataac tatgtctgcc cggagatcga ggtatcgcac acgctcacga tcgccgaggg
    13801 ccgccacccg gtcgttgagc agatgcagcg cgacacgctc ttcgtgccga acgacacgca
    13861 cctgaacgac accgacgacc gcgtcgccat cgtgacgggg ccgaacatgg ccggtaaaag
    13921 cacctacatg cgccagacgg ccatcatcgt gctcatggcg cagatcggct cgttcgttcc
    13981 ggcgcgcagc gcaacggtcg gcatctgcga ccgcgtgttc acgcgcatcg gcgcgagcga
    14041 cgacctggcg ggcggcgcat cgacgttcat ggtcgagatg agcgaggtcg catccatatt
    14101 gaagaacgca accgccgatt ccctgctcat ccttgacgag atcgggcgcg gcacgtccac
    14161 ctacgacggc atggccatcg cccgcgcggt gctcgaatac tgcgccgacg cgagaaagct
    14221 cggcgcaaag acgatgttcg ccacgcacta ccacgagctc tccgcgcttg aggggacgat
    14281 ccccggtgtg cgcaactata atatcagcgc caagcggcag aacgggaagc tactcttcct
    14341 gcgcaagatc ctgcccggcg ccgccgacga gagctacggc atcgaggtcg caaagctcgc
    14401 cggcgtgccg gagagcgtca tccgcgctgc ggacgcgcat ctcagggagc tcaacgcgca
    14461 gggcgcggcg gtaaaggtga cgcccccggc aaagcccgag agcgatcagg taagtctcgc
    14521 cgacgtcggc gcggacgccg tggctgaaag gctgcgcgcc gtcgatccca acacgctctc
    14581 cccgctggag gcgctccggc tgctctttga actcaaaaac gatctcggat gataaaggag
    14641 aaaccgtgat gaaaaaaggc gaaaaggtgc ttcttgtcct cggcgcgctg ctcattgcgg
    14701 cgggcgtgtt cgtcggcgtg acgctcggct gggactattc tcccgacaat tatgcctaca
    14761 acgatattgc atttccctct gccgatacgg agaccggcga gtgcagcttc gtgagcgtgc
    14821 agctcagcga gcttgaggtg aacatctatt ctacgacgga taagaccgtc tcgatcgccg
    14881 cgcggggcgc gaaaacgccg gaccgcgtga gcgttgtgct cgacggcggc gtgcttcgca
    14941 tcacggagaa aaagcccggc cttttccagc gccttgccgt ctcggacgat gatgcggtca
    15001 tcctccgcat tccggaaaaa tgggtcggca cggtggacgc ggcaacgcag tccggcgata
    15061 tatatgtgag cgggctggtg agcccgcagc ttacgctccg cgtatcgagc gcttccggcg
    15121 cggtcagcgc gtcggagctg aaggtcgcgg agacacagct cgtcacgacg agcggaaata
    15181 tttttgtcag cacgacccgc ggcggcacga taacagccgg cacgacctcg ggatatatga
    15241 atatttctga tgtgaaggcg gagagcgcga gcctttcgtc cgtgagcggg agcatttccg
    15301 tacggaacgc ggagctcacc ggcgtgctga cgctgcacac cacgagcggc gatattgacc
    15361 tggacgacat ctccgccgcg ggcatcgaga ccgacacgac aagcggcgat ctggagctgg
    15421 acgaggtcga tacgcagagc cttacgttca cctccacgag cggcgacatc tccggcgagc
    15481 tgctgcacgc ggatgaattc ggcggcacgg tcaccaccgt gagcggcgac atctccggca
    15541 tccgcagcgg cagcgagggt acacggacat tcaagatcga aaccgtcagc ggagacatag
    15601 acctcgacga ctgaataaag ccatgcaaaa cttccccgcg gaacggcctc ctcatggaag
    15661 agccggcccg gcggagagaa aaaagagacg cccccgaaga catatgccag acggcgaaag
    15721 ccgacagggc acggccgccg gnnnnnnnnn ntatgccggc ggccgtggcg taacaaaaag
    15781 atgtttcccg ctttcctttc ggaaagcggg aaacatcttt attatcgcat attatcgcag
    15841 ctttttgagc tcctcaagca ccgaatacgg tattgcgaga ttgccgcagc ccgtgcccat
    15901 ggagatgtgc gttttgcgtg tgccgtggta gtcgctgccg ccggaaacgc ccatgccgta
    15961 tttctccgcg gcgcgcagca gccacgcggt ctgctcggcg gagaactcgg agtaatacgc
    16021 ctccagcgcc cggatgccga gggcctttgc cgtttcgatc agctcgatga gctccggctc
    16081 gggatagtgg tactgcatcg ggtgcgccag caccggcacg ccgcccgcgg cgacgatcgt
    16141 ttccacggcc ttcgcaatcg agatgcggcg cttcggcaga tagtacggct tgccctcgcc
    16201 gaggtacttt tcaaacccct ccttcacgga ggagatgtag cccttgcgca tcagatgctc
    16261 gcagaaatgc gggcggccaa gtacggaatc cgggtattcc tcgcgcagct gcgcgatgga
    16321 gatatcaaac ccgtcgcgct cgaacatggc ggcgatcttc tcgttgcgct cgtcccgctc
    16381 ggtcttcacc cagtcgagca ccgggcgcag ctcgggcgcg gcggggtcga tgaagtagcc
    16441 gaggatgtgg acgttgttgt cgcggtagtc cgaggatacc tcgatccccg gcacgacctc
    16501 cacgccgagc tccgctccgg cgcgcatcgc ctccggcacg ccggagacgc tgtcgtggtc
    16561 ggtgatgctc acggcggcaa ggccgagaga tgcggccttc tccacggcgg cgcgcggcga
    16621 atccgtgccg tccgaggcgg tggtgtggat atgcaggtca atgcagttca tggatgggtg
    16681 cgcttccttt tcgcgaaaat atcgggctcg tcagggcttc cagttgtagc cgccgttatt
    16741 ctggccgttg ttctggccat acgggttctg tccgccgtac ggattctgac cgtagccgcc
    16801 ctgtccgtag ccgccgtagc cgccgccgtt ggtggggtac ttggggctgt cgccgtactg
    16861 gttccagccg ggctggctgt ccttgcagag ccagatgagc agaatgatct gtccgacaag
    16921 ggggatgagg ttcaggaagt accacgcgcc gctgcggccg atgtcgtgca gacgccgcca
    16981 gcagacgccg agtcccggca ggaacacgcc gatgttccag agcgtgagaa acagcgaagc
    17041 aaggctgctg tcaaagttgc gggctatcgc cgtgagtacc gcgctcacgg cccagttgag
    17101 cagcgcgaag taccagtact cgctgcgccg cgcgcggccg gaaaagcaga tgtacttctg
    17161 gaatacggat tggatcgctt cctgaaaact cataaatgat tctcctttat ataatttgta
    17221 aggatatagt agcgtacttc gcaccggatt gcaagaaaga accgcgcccc ggcagggttt
    17281 tctgccggag cgcggttctt tctttacttt ttgtttccga agcactcgtc gtagtgcgcg
    17341 agcgcgctct tgatgatctc cacggcggct tcgcggccgt tcacgtcgcc cgcgaccgcc
    17401 tccttgccct ccagcgcctt gtagacggtg aaaaagtgcg ccatctcgtc aaacgtgtgg
    17461 tcgggcaggt ccatgatatc gcggtatttg ttgtagctcg ggtcggagaa ggggatggcg
    17521 atgatcttct cgtcgttctt tccgcagtcg agcatggata tgtacccgat cgggtagcac
    17581 cgcacgagcg tgagcgggtc gagcacctcc gagcagagca cgagcacgtc gagcgggtcg
    17641 ccgtcgtcgc cgtagctgcg ggggatgaag ccgtagttgg cggggtagtg ggtcgatgta
    17701 tacagaatgc ggtcgagaat gatcagcccg gtctgcttgt ccagttcata tttcttcttg
    17761 ctgcccttgg ggatctcgat cacggcgaga aaatcctccg gcttgatgcg cgcggggtcg
    17821 atgtcgtgcc agacgttcat ggtgttacct ccgggaaaat agtaccgcct tacggcggaa
    17881 ttgaattata tcacgaaggt gtttgttttt caacatagaa agaaaaacga agtcggccct
    17941 taggggcgtt attcctcaaa aaatatcccg gcgtcctgcc ggagagcaag atcgcattcc
    18001 gcttctgcgc acttggtgat cccggcgatg acggggcagg cggcgtgaac ggggaaatgc
    18061 tccgcggcgt aggcgtaaaa cggccccgtc cccggcctgg tgagacacac gtcaaacgcg
    18121 ctgaatgtgc tgccgagagc cttcatcatc tcctgcacgg cggcgcagcc gctcgccacg
    18181 cgcagatgca ccacaaaatc ctccgccgct tccgcctccg cgatggccgt aaagccgcag
    18241 atgcccggct cgatgcgtac ctttgtcatg ggggatcctc ccgttcgttt ttttctcatt
    18301 ataggcgggg agaagccggg gcgtctgggg gtatccggac catatttggc aaatcgtttg
    18361 ccaaatgcgc cggaaaaaag agaggaagca gggttattcc tgcttcctct caaaaaggct
    18421 tattcgttac tcagcgggat aaaaggcatc cttgccaaga ccgcagatgg ggcagaccca
    18481 atcttcggga acgtcctccc aaactgtgcc gggggctaca ccgttgtcgg ggtcgcccag
    18541 ggcggggtca tagacatagc cgcaggggca ctcgtatttc atgggaatat ctcctcttac
    18601 ttgccgaagt agcgcttgag cagaccctcg aacgccttgc cgtggcgggc ctcgtcgcgc
    18661 gccatttcgt gcacggtgtc gtggatggcg tcgagattgt acttcttcgc gagcttggcg
    18721 agctcaaact tgccggcggt cgcgccgttc tcggcgtcaa cgcgcatttc gagattcttc
    18781 ttcgtggaag tggtcacgac ctcgccgagg agctcggcga acttcgcggc gtgctcggcc
    18841 tcttccagac cggccttttc ccagtacatg ccgatctccg gatagccctc gcggtgcgca
    18901 acacgcgcca tggccagata catgccgacc tcgctgcact cgccctcaaa gttggcgcgc
    18961 agaccgtcga tgatctc
//
LOCUS       DAWAAE010000022        18903 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1311_length_18903_cov_3.465089, whole genome shotgun sequence.
ACCESSION   DAWAAE010000022 DAWAAE010000000
VERSION     DAWAAE010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 18903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18903
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_18903_cov_3.465089"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3643..3652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8384..8393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9268..9277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3566 a         5765 c         6031 g         3511 t
ORIGIN      
        1 aacgaccgcc gcgtgggagg tcatgccgcc gcggacggtg aggatgccct gggcggcctt
       61 catgccggtg atgtcctcgg gagaggtctc aagacggacg agaacgacct tctcgccgcg
      121 cttcttccac tcgatggcgt cgtcggcgtt gaagacgatc ttgccgcagg cagcgcccgg
      181 gctggcgccg aggcccttgc ccatcgggtt cgccttggcg agcgcttcgg cgtcgaactg
      241 cgggtggagc agggtgtcaa ggttgcgcgg gtcgatcatc agaacggcct gcttctcatc
      301 gatcatgcct tcatcgacga ggtcgcacgc gatcttgagc gcggcctgcg cggtgcgctt
      361 gccgttgcgg gtctggagca tgaagagctt gccgttctca acggtgaact ccatgtcctg
      421 catatcgtga tagtggttct ccagcgtgga gcagacctgc acgaactggt ggaacgcttc
      481 ggggaacttc tcttccatct gggcgatggg catcggggtg cggacgccgg cgacgacgtc
      541 ttcgccctgc gcgttggtga ggaactcacc catgagcttc ttctcgccgg tggcggggtc
      601 gcgcgtgaag gcaacgccgg tgccggagtt gtcgttcagg ttgccgaaca ccatcggcat
      661 gacgttgacg gcagtgcccc aggagtaagg gatgtcgttg tcgcggcggt aaacgttcgc
      721 gcgcgggttg tcccaggagc ggaacacggc cttgatggct tcgtacagct gggtcttcgg
      781 gtcggacggg aagtcctcgc cgatctgctt cttgtactcg gccttgaact gctcggcgag
      841 ctccttgagg tcggcggcgg tcagctcgac gtcgagcttg acgcccttct tggtcttcat
      901 ctcgtcgatg agctgctcaa agtacttctt gccgacttcc atgacgacgt cggagaacat
      961 ctggatgaag cggcggtagg agtcgtaaac aaagcgctca aacttgggat ccgggttgcc
     1021 ctggatcatg gcgttgacga cttcgtcgtt caggccgagg ttcagaatgg tgtccatcat
     1081 gccgggcatg gaagcgcggg cgccggaacg gacggaaacg agcatcgggt tcttgagatc
     1141 gccgaacttc ttgccgttga tctcttccat cttggcgatg ttctccatga tctccgccat
     1201 gatctcgtcg ttgatcttgc ggccgtcctc atagtacttg gtgcacgcct cggtggaaat
     1261 cgtgaagccc tgcggaacag ggagaccgat gttggtcatt tcggcaaggt tcgcgccttt
     1321 gccgccgaga agctcgcgca tggaagcgtt gccctcgctg aacaggtaaa cgtacttgtg
     1381 gctcatggga taacctcctg tatataaatt acttagtatt gcggacgatt cccagggtag
     1441 atttactttt caaatcatta tgcccgaaat cagtttcgca ttatatcatt atttcggaca
     1501 aaaaccaata ccaaaaatga aatttttaaa tcgtttccac ggattatttt ttgattttga
     1561 aagaaccctc gcaaagcctg cacccgcggg tgcgggacag ggggtcattt gccgacacag
     1621 aagcgcgaga agatctcctg cgtcacatcc tcgcgcaccg accggcccga gagcgcgccg
     1681 agcgcgtcca tcgcgccctc gcactcggtg agcaccgcgt cgggcgcaaa gcccgccgcc
     1741 atcgcctcgc gcgcggcgcg aagatactcg agcgcgcggc gcacggcgtc tgcctggcgc
     1801 gcgttcgtca gtatctcgcc gctgtccgcc tccggcatcg ggaacgccgc cgcgaccgcc
     1861 gcgccgagcg cgtccagccc cgtcttttcc tttgcggaaa cgacgcagac gctgtcaaaa
     1921 tgcgccgaaa ggcgcgagac gtcccaccgg ggggcgcagt cgctcttgtt cgccgcggcg
     1981 atgcgcacgg cggccttttc cgcgatggcg agcaccgcct cgtcctcggc gtcccagggg
     2041 cgggagagat cgaacacggc aataacgagc tgcgcttcct ccgccgcggc gatggcgcgc
     2101 tccacgccga gcgattccac ggcgtcggat gtttcgcgca cgcccgcggt gtcggtcagc
     2161 cgcagcgcca cgccgccgag aaggcaccgc tccgagagcg tgtcgcgcgt cgtgccggga
     2221 atgtcggtga cgatggcgcg ctcaaagccg agcagcgcgt tgagcagcga agattttccg
     2281 gcgttcgggc ggccgagaat ggccgtgcgc acgccctttt caagaaactg cccgcgggaa
     2341 aacgtggcga gcagcgcttc gagcgagcgg atcgacgcat cgagcacggc ggcgtattcc
     2401 gcgagcgtga acggcggtat atcctcgtcc ggatagtcga gcaccgcgtg atagtgcgag
     2461 cagatgtgcg tgagattctg atagatctcc tccgtgcggc gggagatcgc cccgccgagc
     2521 tgactggcgg catttcgcgc cgcgagaggc gtttccgcgt cgatgatatc gacgacggcc
     2581 tccgcctgcg tgagatccat gcgcccgttc agaaaggcgc gcttggtgaa ctcacccgcc
     2641 gctgcctgcc gcgcgccgag ggcgaagaga gcttccagcg cggagcgcag cacgacgggc
     2701 gagccatggc actgaagctc tgcggtgtcc tcgccggtgt agcttcgcgg cgcgcgggag
     2761 agcgtgcaga gacagagatc gagcgtccgt cccttctcgt cgagcagctt gccgtaaacg
     2821 agctgccggt cgcggtgctg cgacatggcc tttccgtccg ccgggcggaa aagcgcgtcc
     2881 atcagcgata cggaaagggg gcccgatacg cgcacgatgc cgatggcgga aacgcctgcg
     2941 ccggtcgcaa cggcggcaat ggtatcactc atttatcgtc cttctttgcc caggggtcgt
     3001 ttttgctctc gtcgtaggcg tccttgaatt cctccaacgt gatgcccttg cgcatgatgt
     3061 attccgcggc gacctcgccg acatagcgga aatggccggg ctccgggtcg cggccggtat
     3121 attcctcacg accttcggga tagcgcagga tgaagccgta ttccgcgcag tgctcgcgca
     3181 tccattccag cacgggcgtg gagaatttcg cctcggcgtc ctcgccgacg aagttgacgg
     3241 caaggccggt ctggtgctcg tccgcgccgg ggtagtagcc catctccgtg accttctttt
     3301 ccgcgtctgc gagcgatata tcgccttgat cgtaaagcgt actcgccatg ccgttgaagc
     3361 gcgtgcgctg gtcggggtag gacatatagg tgcgcgaaat gctcaccgtg aagccctgcg
     3421 cgcgggccgc ggcgagaaat tccttgagcg cgtcggcggc gcggcgctgg aagaagtagc
     3481 cgttttcgat ctcgaccatg tcggggtagt agttttcacg caggtagttc tcctgcgaaa
     3541 ggccgttcac gagcagatac cgccagttgg aaagaccggg ctgctcctcg tcgccgagcg
     3601 tttccttatc cggaagatcg gaataatcga cccagccggg ggnnnnnnnn nngccggggg
     3661 gatactgcca gttgagctcg ccctgcgcgt cttccgcttc cggaacgggg gtcgcggcgg
     3721 tctcgggcat gggggcgctc tcctccgtgg agagagagct gtcggtattc ggacggagca
     3781 gcacgaacag caccgccgcg agcgccacgc cgccgacgat gatgcgccgg gcgcggagga
     3841 ccttcgggtt tttcggtttg attcgcatat agcctccttt ctccccgcgc gaagcggggc
     3901 gttcccggaa gaaaaagcgc gggaacggga agagcggggt tgacattccc cgcgaatact
     3961 ttatataata tcgagtacta tccctgcgcc gctgccggcg ggcggggacg attttatgcg
     4021 ggtatgcgga aaattcataa tattatacca ttttatttgg aaagatgcaa gttttccaag
     4081 cataccgccg agaaggagac acatccatgc tggagctgaa gaatatacac tattccgtgg
     4141 acgcgccgga gggccgcacc gagatactgc gggacatttc cctcaccatc ccggacaaaa
     4201 agctcatcgt tctcaccggg ccgaacggcg gcggcaaaac gacgcttgcc aaggcgatca
     4261 tgggccttgt gatgccgaca ggcggcagca tcctctggaa cggcgaggat gtgaccggcc
     4321 tttccatcac cgagcgtgcg cgccgcggca tcagctacgg cttccagcag ccgccgcgct
     4381 tcaagggcat ccgcgtgaag gatcttttga cgatcgcgtc gggacagaag ttcctgccgc
     4441 ggggcgaggc ctgcaagtat ctcacgcagg tcggcctgtg cgcggccgat tatcttgacc
     4501 gcgaggtgga cgcctccctc tccggcggcg aggtcaagcg catcgagatc gccacgatcc
     4561 tcgcgcggca gccgcagctc atgatcctcg acgagcccga ggcgggcatc gatctctgga
     4621 gcttcgcacg ccttacggaa acgtttgagg agatccacac ccggcaggac tgcacgatgg
     4681 tcatcatctc ccatcaggag cgcatcatct cgctggcgga cgagatcctt gtgatcgaca
     4741 agggagtgat cgcgcgccgc ggcacacgcg acgaggtctt taccgacatc gtccacagca
     4801 ccacgggcgg ctgcccggtc atctacgggg aggcgaagaa atgagcgagc tcaaccgcgt
     4861 cgatacgacg cttctggaag tcattgccgg catgaaggac ggcaaggccg agggcgcatt
     4921 caatatccgc aaggactccg gctgcgccgg gcgcgtcaac accgaaaacg tcaccatcac
     4981 cacgaaaacc gataagcccg gcatcgatat ccgttttgtc cccggctgca aggacagctg
     5041 ccatatcccg gtcatcatca cggagtctgg cctgcgcgaa acggtgtaca acgattttta
     5101 cgtcggcgag ggcgcggacg tcacgatcat tgccggctgc ggcatccaca actgcggctc
     5161 ggacgcctcc gagcacgacg gcgtgcacac gttctacgtc ggcaaaaacg cccgcgtcaa
     5221 gtacgttgag aagcactacg gcgagggcga cggcaacggc aacggcaaaa acgttatgaa
     5281 ccccacgacg atcgtctatc ttgaagaggg cgcgtcgata cagatggaca tgagtcagat
     5341 cggcggcatc gactccacgc ggcgcgatac gcgcatcgtc tgcggcaagg atgcggacgt
     5401 cgtcattacc gagcgccttc tcacccacgg cagtcagcgc gccgagtctg acatggtcat
     5461 tgagctcaac ggcgagaact cccgcggccg cgtgatctcc cgaagcgttg cgaaaaacga
     5521 ctccgtgcag gtgttccgcc cgtgcgtcgt cggcaacgcc aagtgcttcg gccatgtgca
     5581 gtgtgactcc atcatcatgg acgaggcgaa gatccgctcc gtgccggaga tcgcggcgaa
     5641 cgatctcgac gcgcagctca tccacgaggc cgccatcggc cgcatcgcgg gcgaccagct
     5701 tttgaagctc gaaacgctcg gcctaaccga ggaggaagcg gaggaccgca tcctcaaggg
     5761 cttccttgcg taagaaattt tataaggcaa agagccggtg acgggaagtc accggctctt
     5821 tgttatatta aaccgaaaag aatttatccc cctggggggc ttccctccgg gggtggatag
     5881 ctttataatt aaaagaccat tatacagaga tgccgatcaa aaagagaaag gagcgaacgg
     5941 ccatgaaagc aaagtcccgc gcccggtata ttatcgccgc gcttgcgctc gcggtgctta
     6001 ttatggcgct cgtctgcctt tttgtcggcg catcgcgcat gagcttttcg gacggcgtcg
     6061 ccgctctttt cggaaaaggc tccgccgcta acgtgcgcat cctccgctcg atccgcgtgc
     6121 cgcgcgtgct cgcggcgctc atcgccgggg ccgcgctctc ggtctccggt ctcgtcatgc
     6181 agacgacgct ctcgaacgcg atggcctcgc cctccacgat gggcgtgtcc aatgccgcgg
     6241 tgttcggcgc gaatctctcc atcatcgtgt ttgccggggg ctttctctcc acgggcaaca
     6301 atcttgccag ctataccgcc ggggtgaacc cgttcgccgc gagcgtgacg gccttcctct
     6361 tctcggcgct ttccattctg ctcattctcg cgctctgccg cgtgaaggcg ttctcgccga
     6421 acgtcgtggt gctcgcgggc atcgccgtcg gcgcgatctg gacggcggca acgacgcttc
     6481 tgcagtttta cgccacggac gtcggccttt ccgccgcggt cgtgtggaat ttcggcgatc
     6541 tcggccgcgc gacatataag accgaccttc tcatgctctg cgccgcggcg ccggggctgg
     6601 cggtattttc cgcgctcgcg tggcggtata atgccctttt gagcggcgac gccgctgcgg
     6661 gcagcatggg cgtgaacgtt tcgcgcctgc gctttttttc gctgctgctc gcctcgctga
     6721 tgacggcggc gtgcgtatcg agcctcggca tcatcggctt tgtcggcatc atctgcccgc
     6781 acgtggcaaa gcgtctgctc gggcaggatc accgcgtttc catccccgcg tcggcgctcc
     6841 tggggagcct gctccttctc tgcgcggacg cgctctcccg ctcgctcggc agcggcagcg
     6901 cgctgcccgt cggcgcgatc acgtcgctgc tcggcgcgcc gttcttcctt acgatcatct
     6961 tttcgcggaa ggagcacggc caatgttaga gctggaaaat gtagtctttc ggtattcccg
     7021 ccgggccgag ccggtgctcc ggggtgtttc gctcacgctc aacgacggcg agatcgggat
     7081 ccttctcggc aaaaacggcg cgggcaaaac gacgcttttt cagtgcattc tcggccttgc
     7141 cgcgccggag agcggctcga tccgcttcga cggcggggag cttttgaaaa tgccgcggcg
     7201 cgagagggca aagctcgtcg cttatgtgcc gcagcacatc catttcggcg cgctgagcgt
     7261 gttcgactcg atcctgctcg ggcgggtctc ccgctttggc atgcagccgg gacgcgagga
     7321 tatcgaggcc gccgagcaca ttctcgccga gatgcatctt gagccgcttg ccaaccgcag
     7381 cgcggaggaa ctctccggcg gcgagaagca gaagatcgcc atcgcccgcg cgctcgtgca
     7441 ggagccgcgt ctgctcattt tcgatgagcc gaccggcaat ctcgatatcg caaacgaaga
     7501 gctcatcatc gacgaggcga aaaaggcggc gcggcagcgc ggcgtcgcca ttttgagctc
     7561 gctgcacgat ctcaaccagg cgctgtgcct gggcgataag tttttcctgc tccgggacgg
     7621 cgcggtgcgc tacgccggag acgagagcgt actcacgagc gaagtgatcg gggacatttt
     7681 cgatattcaa tcccgcatca tcaccgtaga cggaaagaaa gttattttgg gaggaaaata
     7741 ctatgaagaa agctaagctc ctttctctgc tgctcgcgct tgcaatgctc ctttcacttg
     7801 ccgcgtgcgg cgcggccccg accgagaccc cggcggccac ggaagctccc acggaagcac
     7861 cggtcgaagc gacggaagcg cccgccgaga gcgcggagat caccgttacc gacctcatcg
     7921 gccgggagat caccgtgaag ccgggctcgt atcagcgcgt cgtgtgcatc ggcgcgggcg
     7981 cgctgcggct ctacagctac atcggcgacg tatccctgct ctgcggcgtg gaggatatcg
     8041 acaacgaaac gctctccgag cgcccgaaga tgttcgattc cgtcgcgcgg ccctatgtgc
     8101 tcgcccacag cgatatgttc gcgtcgctgc cctcctgcgg cgtcggcggg ccgaatgccc
     8161 agagtcccga ggcggagaaa atcctcacct gcgagccgga tatcgtcatc tccctttacg
     8221 gcgatgcgga taaggccaac gccctgcagg agcagctcgg cgtcccggtc gtaacgctga
     8281 tgagcggtcc ggacagcgtg tttgacgaga gattcaacga aagcgtccgc cttctcggca
     8341 cgatctttga agagagcgag aaggcagagg cactcatcgg cttnnnnnnn nnnatcgaag
     8401 cgcgcaccgc cgatatcgcc gaagaggaca agcccgccat ttatatctgc ggtctcggca
     8461 actggggcac gacgaaccac ctgatgaccg cgcaggacta tgtttccttc cgcgtggcga
     8521 acgtgaagaa cgttgtctcc gaccttggag cgcccggcgc gcagccgatc gaggcggaga
     8581 aattcgtcgc gctcggcgag gacatggacg ccatgatctt tgacgccgcc gccgtgaaga
     8641 acatcaagcc tctttacgcc gaggatccca cgatgttcga tacctgccgc gcctggcgcg
     8701 agggcgaggc gtatctccag cttgcctaca acgcctacta taccaactat gagatcgcgc
     8761 tcatcaacac atggttcgcc gcgaagaccg cctatcccga tcactttgag gatgttgaca
     8821 tgaccgaagt gacggaccgc gtcacgaccg cgttcctcgg aaaacctttg gccgaagaga
     8881 tctatgcctg cccgaacagc ttcggcggct accagaagat cgacaccgcc acgttctttg
     8941 tggggacgca gggatgatcg agcagaagga tgtgatcgcg ttcttcgatg agcgcgccgg
     9001 agaatgggac gccggtatgg tccgcagcga cgagaagatc ggccgcattc tcgataacgc
     9061 gaaggtgacg gtgggcagcc gcgtgctgga cattgcctgc ggcacgggcg tgctcatcgg
     9121 cgactatctg cgccggaacg tcgagagcgt caccgccgtg gacatctccc ccgagatgat
     9181 ccgcatcgcg cgcgaaaaat tcccgcagga gaacgtgcgc ttcgtctgcg gcgacgcgga
     9241 gaccgcgccg ctcggcgagg gctttgannn nnnnnnngct caccgtggcg cacggcagct
     9301 cgcgcgcgga catcgacgcg caccaccacg gcgcggcgag ccgtgtttcc aacgggctca
     9361 tggaggccga agcgctcgcg gcgatcttcg cgcggcacct tcgcgtgacg gatgttatct
     9421 cgaccgacga aatgtatcag gtcgtgggcg aaaaagaata aagcgataaa aagtgccgca
     9481 ggagcaagag acctctctct tgtcctgcgg cgctttttct gatacgatgg gattataaaa
     9541 aacagggagg ccgctatgaa gatctgttcg gtatacgatg cggagttcgc gcgctacggg
     9601 cgcgtgcatt ccggtataga gagcgcggcg ctgctgcgcg aaatggagaa gatcctcctg
     9661 ccggagagcg gcacggcgta tgagccgtcc gttgctgcgc tcgaggcgtg cgaagcgttc
     9721 gcggactatc ggacgagtgt gttcggcggc atgccggtgc agctcggcat gtgctggggg
     9781 cgcaatacaa agcttaactg ccttgaatac caccgcgaca gcgaattcaa tctcggtctc
     9841 ggcgacttta tccttctgct tgccaagcag gacgagatcg aagacggcgt gctcgacacc
     9901 gcgaaggtga aggcgttccg cgtccccgcc ggggtgctcg tggaggtcta cgccacgacg
     9961 ctgcactatg ccccctgcca cacggacgaa acggcgggct ttcgcgtgct cgtcgcgctg
    10021 ccgcgcggga cgaatacgga aaagcccgcg ctgcgcggcg gctcgccgga ggacaaatat
    10081 ctcgccgcct gcaacaagtg gctgctcgcg cactctgaaa gcgccgaggc gaagaacggc
    10141 gcggcagtcg cgctgcgcgg cgagaacatc gatatcgccc cggagcttca agcgccgatg
    10201 gatatcgcgg ataagatcat cgaagcgctg cgggagaaat accatccgct cggcatcgcc
    10261 gtatacggct cgtttgccga cggctcgaac aaccggaaca gcgactttga cgcgctgctc
    10321 ctgctgccgg acggcgagac cggacacgac gattcggtgc ttttcggtac ggagctggac
    10381 gtctggctct acggcgcggc gcatttcgcc gcgccgtatg acgcggagga ttttttgcag
    10441 ctccatgaca gcgtgatcgt tgaggatgtg accgggcggc tctcggcgct ccgggccgag
    10501 gtaaacgccc atatcgagag cgccccgcaa aagaccgagg cggaaaacgc gcagtcgctc
    10561 tcgtggtgct ggaaaatgct ccggcgcacg gagcgcggcg acgcggaagg ctgctaccgg
    10621 ctgcactggc tgcttacgga cagcctgagt atctattatg atctgcgcgg cgagtattac
    10681 ttcgggccaa aaaaggcgct gcgccggatg gcaaataccg cgccggacga cgccgcggtc
    10741 tatgagcgcg ccctgcacga gccgtcgccg gaaaatctcg ccgcgtgggt cggcgtattg
    10801 gaagagacgt tttcccggag ataccggccg tgagggatag aaagcaccgg taaaatgttg
    10861 ataatctgag ataatcggaa aagcgagact tcttccacag aagtctcgct tttactgtct
    10921 tttttcagag cggctgcttt ttaatcatgg cgaaccggcg gggatatttc gccggggtgg
    10981 gcgcggtttt cgggatgata acgacgctgt gcacggcgtc cgtgccggga acggtgtatt
    11041 tctcgatgcg cggttttccg ccgccgagcg tgcggatgca gcgcgccgcc tcctgcacct
    11101 cttcctccgg ctccggcccc ttcatcgcgg caaacacgcc gccgacgcgc acgagcggca
    11161 ggcagagctc gcagagcatc gtcatgcgcg ccacggcgcg ggatgtgacg acttcaaagc
    11221 tctcgcgcag cgcgctctct tcctcggcac gcccggcaag gcatgtgacg ttcccgagac
    11281 cgaccgtatc gcacacctcc tgctggaacg tcacgcgctt ctggagactg tcgatgagcg
    11341 tgatgcccgc gtccggcgcg aggatcgccg cgacaagtcc cggaaagccc gcgccggagc
    11401 cgatgtccgc ggcggtcttg ccggagagac gcacataccg gagcagcgcg gcactgtcga
    11461 gaaagtgcag gcgcgccgtc gcctcctcgc cctcgatggc ggtgagattc atcaccttcg
    11521 agcgctcgtc gagcagcgta tagtaggccc ggagctgcga gagctgcgcc gccgtcgccg
    11581 ggacgccgag ctccgcaagg ccggatgtga gtatttcttc catggtcatg gtgtccctcc
    11641 agaagagcgt tgctatgaac ggagaataaa cccctccgtc aaaaacttcg tttttgccac
    11701 ctcccctgac gaggggaggc aagcaggttt gtgaattact atcttggccc ccctaacagg
    11761 ggggctggca gccgttaggc tgactgaggg gttttcccgt cagctcttcc ggtcgaatcg
    11821 gtgcccgcag cgggggcagg tgacctgaat gcggccctta ccgcgcggca cgcgcagata
    11881 gctgctgcag ccgggacagg agaaaaaccg gaaatcgcgg ctctgcttcg cgcgatgcag
    11941 cgcaccggaa atcttttcct tcgcgcggtc gtatttctcg cgcgcgacca tgtcctccgc
    12001 ctgccgcatg ggaagattgc gggagagcgt ccggaacagg gcgtagcccg atataagaaa
    12061 cgggacggag ccgatccgcc ccggaaacag cagattcagc ccggcgatga tggccgcaat
    12121 gcggaagaga tgcttattga gaaagtccac gccgtttcgt ccgagcatga acatattcag
    12181 ccgtgtccgc atagtccgcg ccccctttcg tccggatatt ttataattct atcatgccgg
    12241 ggcgaaaggc gtcaacaagc cgttggcata ttctctgccg ccgcaccata agatagatag
    12301 tatcgatggg caggggaggc ggaacgatga accggaaggg aaagctgctg tgcacggcaa
    12361 ttatggcggc gcagctggtg tttcatgccg gggcgctggc ccagccggtc gagcgcgccg
    12421 ccgagacata tgaaagcggg cgtctcggcg cggcgctgct cgcgctcgaa ctcggcgacg
    12481 cgcacgcttc cggacagctc ctgctcgcgg acaaccttcc gctcgaagcg tcggcaccgg
    12541 agaccacacc ggagccgacg gcggaaccgt cgccggaact gacaccggaa ccaacgccgg
    12601 agccgacgcc cgaacccacc ccggaaccga cgccggagcc gacgcccgaa cccaccccgg
    12661 aaccgacgcc ggagccgacg ccggagatca cgcccgagcc cacgagcgag ccgacgccgg
    12721 agatcgtgcc gttcacggac gacaccggca tggagatccg gaacaaaacg gaccttgaag
    12781 cggatatcgc cgcgctcatg gccgagccgc tcgtgcagaa gctcggcgcg gaggggccgc
    12841 agatcttgat aatacatacg cacggcacgg aggcatatca ccccgcggcg ggcgaggaat
    12901 atacgccgag cgaccccttc cgcacgaccg atccggggcg ctcggtcatc cgcgtgggcg
    12961 acgcgctgtg cgccgtgctg gaggaatacg gtcttcgcgt gctgcacgac cgcacgctct
    13021 acgactatcc gagctacgcg ggctcttatg agcgcgccgc ggcgtccatc gaggcgtatc
    13081 ttgccgagta tccggacatc gcgctcgtgc tggatgtcca ccgcgacgcc atcggcgacg
    13141 aatcgaccgt ctataagacc tgcgccgccg gaagcggcat ggcgcaggtg atgctcgtgt
    13201 gcggcacgga cgaatatctc gagcacccgc tctggcggga aaatctcaag ctcgcgctcg
    13261 cgatgcaggc cgcggcggta tcggcaaacg gcgcgctcat gcgcccgatc catctcgcgc
    13321 cggagcgcta caaccagcag tatacgaccg gctctctcat tatagaagtc ggcacgaacg
    13381 gcaacacgct ctccgaagcg ctcgccgccg cggagctttt cggccgcgcc gcgggggaaa
    13441 tgctcgcctc tctcgccgcg ccgggttaag agccgcgccg attgtccata ctccctgtaa
    13501 ggaacaaacc ggacagggag gatatatttc ttatggaagt tcaggagatc atgacggcaa
    13561 aggtcatcac cgtcggacag gacgagccgg tcacggcggc ggcgcggctg ctgcggcggt
    13621 ggaatctcgg cgcgctgccc gtgacggaca gcaccgggcg gctgcgcggc gtgctcaccg
    13681 accgggacat cgtgctgcgc tgtcttgcgc tggataacga ccccgcggac acgcgcgttt
    13741 cggaggtcat gtcccgcgcg gtcgtgacgg cgcggccggg cgacagcgtg cagagcgccg
    13801 ccgcgctcat ggcgcgcgag cagctgcgcc gtctgcccgt ggcggaaaac gggcggctcg
    13861 tcggcatggt gacggcgtgc gatctcgccc ggcgtgaaaa gctcgacgcc gaatttgccg
    13921 aagccatgcg cggcatcagc gccaatctgg tgcggagata aggcagaagc cgccggtcag
    13981 agtcggtcgc gcccgaattc ccgatcatcg cggcgcgatc gcgcaccgga tgctccgctc
    14041 cccgcggacg acgctcgccc ccgctgagga ggaagcgctg ccggcacggc cggaggaaat
    14101 ggatcataca aatccttgcg tttgagcgcc gggtacgata aaataaagaa aaacgatata
    14161 ggagcagccg agagatcctt tcttccgtcg gatcccgatc ttcgtgctgc gtctgttctc
    14221 tttgccgggc cgccgcctgc ggctgcatcc tgacgcaggg gcacggcacg atcggggatc
    14281 atgcgtgcga gccgctggtc atgctcaagc acttcggccc caaccatccc gaaaagccgt
    14341 aaaaaagcca aagatataag gaggaaaaag ccatgctcaa ccacatttca ccggtattct
    14401 ctccggaact tctcgctgcg gtctacgcga tgggacacgg ggacacgatc gtgctggcgg
    14461 acgggaactt cccctcggac ggggtgagcc gccgcggcgc ggcggagatc atccgcgcgg
    14521 acggccacgg catcccggag cttctcgacg ccattctggc gctcatgccg ctggacacct
    14581 acgccgaaaa gccggtgctg tacatggcaa agtccgagtg tgaccgcgat cttgcgcttc
    14641 ctattctgga cgaatacaaa aagatcatcg agaagtacga cgagcgcggc gaggcgtgcg
    14701 cgggctatct ggagcggtac gcgttctacg ataaggcgga gcaggcctgc tacatcgtgc
    14761 agagcggcga gacctcgcag tatgcgaacg tcatcctgca gaagggcgtc gtcaagcgga
    14821 ataagtgatc gtttcttttc tcctttcggg gcgtctgcgc cgtttcggcc ggacgccccg
    14881 ttttcgcgtt ttcggcggaa aagaaaaaag gtgtcagcgg gatcgctccg gctgacacct
    14941 ttttgtcggg tcgattacag aacattaaaa ttcatgttca tgatgtactg ctccatgaat
    15001 tcggcgctgg cgttgagctc cacatgatgc gtgacggcac ggatgtgctc gcagcgggcg
    15061 ctctccgcgc ggtcgcggag atagcggcac acgccgctgc ccgcgccgtt gccgacggcg
    15121 acgaccctgt cgcgcggcgc ggcggggaga aggccgatat acaaagcgtt gtccacgtcg
    15181 atgtagtttc cgaacgcccc ggcgaggatg agcgaatcga gctcctccgg ctcaatgccg
    15241 tgctcgtggc agagcgtcag acacccggcg cagatcgagc ttttggcgag ctggatctga
    15301 cggatatcct cctgcgaaag atagaccgga tgctcgctgc gcgtgaggaa gaaggcgttg
    15361 ttgtattcgc ccaccggctc aagccgaccg agcagggggc tgtccgggta ctccgcggcg
    15421 aattcgtcgc gcgggaggag ccgtccggtc atgtccacga ggccgcagcg gacgagcgcg
    15481 gcggtcatgt cgatgatgcc ggagccgcag agccccttcg cctcgccctc gccgaggacg
    15541 tggaagcgaa gctccccgtc cggcgtgatg tctgcgtggt cgatcgcgcc ctccgagccg
    15601 cgcatgccgc attcgatgtt gccgccctca agcgcggggc cgcaggcggt ggaggaggtg
    15661 aggaagccgt cgcgtgcgtt gccgacggcg atctctccgt tcgtgccaag atcgacggcg
    15721 agatgcacgc cttcctccgg cacgccgagc agcacggcgg tcgtatccgc gccgacgaac
    15781 ccgccgagca gcggcagaaa ctccacgagc gcgcccgcgg ggcagttttt cagtccgatc
    15841 tccccggcag gagcgcgcac ggtgtccgcg gtgacgctcg caaacggctc cgcgccgagc
    15901 gcggacgggt caatgccgag gaagagatgc tgcatcgtgc tgttgccgca gaaaacgagc
    15961 tgccagagat cgtccgagag gcccggcacg gcgtttgacg cggcggcgag aagagcgtcg
    16021 atcgtgtcga gaatggcgcg gttgagctcc gcaagcccgg tttcgtgctc tatggcgtgc
    16081 tggatgcgcg cgatgacatc cgcgccgtac cggatctggc cgttgagcgc cgagccggtg
    16141 tggagaagag cgccggtcgt aagatcgtag atatacatgg caacggtcgt tgtgccgatg
    16201 tccacggcgg cgccgtaaag acgctgcgcc gtatcgccgc gctgcacgcc gaggagcgta
    16261 tcctcatggc ggatgaacgt gaacggctct tcctccgcgt cgcggtgcgc gcgggaaaac
    16321 tgccgcgccg ccgcgagcga aacgccgcgg agataagcgc cgcagtgctc cggctcccgc
    16381 tcggcgggag agagaacggc tttcgtcacc gcgggggcga agacatcgtg tgcgccgccg
    16441 gaggtgagga tctgcgcgga ggcgttctcc gaaacttcaa gaaaaacggt gatgtcctcc
    16501 gtgacgggcg tgatgcacgc gcgcacggtc tcgcgcacgc cgcggcgctc cacgagaaca
    16561 tcgcatttcc cgcagcggcc gttgccgccg cagacgagct cgagcgggtg gccaagcagg
    16621 gcgcacgcct cggcaagcgt gccgccggag acgcgcgttt cgcacggcgt tccggcgata
    16681 cgaacggtat attccataaa agcagcctcc tgtttacgga ttcgtgtccc ggcgcatata
    16741 gcacgagaga ttcgggcatt tggagcagtc gtgatcgatg gatcggtcgg ggaggccggt
    16801 gcccgcgccg taggcataaa gcatggcctt ctccggctgg agcatgtagt gctcggtgag
    16861 cgtggcgggg aacggctcgc cctgcgtgag cagggagagg aactgcgcct gatactccat
    16921 gctcaaatgg ttctcgcccg gcgtgaggcg cgtgctgaga tggtagccct gcgccttgaa
    16981 ctcgttataa agctggcggt tcatcgcgtt tgaggcgttg aaaagcacct cgttggcgag
    17041 gtcgttgaga atgtagccct cgagaaaatc gctcttcgcc atgagatcgt ccacgcagtc
    17101 tacgatcttc tgcgtggcgc cgcagtagca caccgcgagc atatcgcagc tcgaaagctc
    17161 cggcgaggcg atggtcaggt cgttgtctgt aatgttgtag gcaagaacaa gctcaatgtt
    17221 ctcgcgcagc agctccacga ggtacgggaa aatgctctcg gcgtaggcgt ttgtgttggg
    17281 atccgcccgc agcttgagcc gtgtgaagat gcgattcttg tcgtatttga gctcatccac
    17341 ggagacgggg aagaacgtca tatcatttac ctcctatctg cggcacgggg aagccgcgga
    17401 tgtactcgtt gtcgagcagc ttgtccgcac cggcggaaac tttgaactgc cgcggggttt
    17461 tccctgtgat ttttttgaaa actttggtaa aatagctctg atcgtcaaag ccgacgcgtc
    17521 cggcgatctc cagaaggttc atgtccggct gcgagagcag ctctacgctc ttctggatgc
    17581 gcaggctgtt gagatagtcc gtaaagccga cgccgagctc ttttttgaag agtttggata
    17641 ggtatgattc gttgatatgg atgctgccgg ccacgtcgcg cagcgtgagc ttttccgcgt
    17701 accgctcgcc gatgagctcg atggcctgcg tgatgagcgg agaatagacg ggcgcgggcg
    17761 gctctggcac gagcgaggaa aagtggtcga tcagcttctg cgtccacacg gccagctcct
    17821 ccaccgactg cgcctggttg agcgcctcga gcgggtcgag cccggcggcg aggatcttgt
    17881 tcagattgat gccggaatca agcgcggcct tggagagcat cgtgaaaagc tcgatgagcc
    17941 gcatgcggat gagatcgaaa tttcccgctt ccacgagaag aaactcgtca aagaggccgg
    18001 aggctgcctc gtttacggcc tcgcggtcgc gctcgcgcag actggcgagc aggcgctgcc
    18061 agagcgtttc ttccgtctcg ccgacggtgc agacggcgag cttgaactgc tgcaccgtct
    18121 ctccggcgct gatctggccg cggctgcggg cgcggatgtg ttcatattcc tcccagttct
    18181 ggcgtgagct gagcaccgag gcgtaaagaa gcgttgcgat cttctgtatc tgctcgggcg
    18241 tgctctcctt ggcgttctgg agaaagagag ccacgcgcgg gaagagcgcc gagtcgtccg
    18301 gattgagctc catgatgcgc gcgagcagct tttcgtccac gctgccgagc acaagcggcc
    18361 cggccagcac gcagcccctg cgcgcaccat tatatagtat gggaacggcg atctcgacga
    18421 agcccgccgg gcagaagaat atgtacggct cgccgagcgc gaaagactca tccatcgaga
    18481 aggtcagata tccctgacag ccctcgctct ggctgaaatg cgggaacagg cggcagacct
    18541 tctgttccgc gttgaattcc cgcgtgatcc ggttttccgt gtcgtacacc gtcacaggga
    18601 taccggtaca gctgctgaaa gcatccagcg cgtcgtagag tctgtccatg ggaaaagccc
    18661 cgccctttct ttctttatta cagcggctat agcgcgttac agcccctata actgcatcat
    18721 aggcgatagc aaaggcgttg taaaacaaaa aatgacgaaa aacaaagatt ttaccaattc
    18781 tgctaaaaat tctacaacaa aaatccgtga tacggtttct attgcggcaa aaacggcaaa
    18841 acacccctcc tgcggcagga gaacctgacc ggtattttac catttttgtc cctctttttc
    18901 aga
//
LOCUS       DAWAAE010000023        18477 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1357_length_18477_cov_3.104766, whole genome shotgun sequence.
ACCESSION   DAWAAE010000023 DAWAAE010000000
VERSION     DAWAAE010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_18477_cov_3.104766"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3807..3816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5333..5342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3890 a         5265 c         5461 g         3831 t
ORIGIN      
        1 gcgtcggggt aggtgtcgga gtaggcgtgg gagtcggtgt cggcattagg gcggcggcgg
       61 agatgacgat atttccggtg atcgccgcgg cggggatcgt cagaatgccg gtcgcgggat
      121 cataggaata cttcccgctg ccgagtaccg tgccgccaat agaaatattc cgaagcgtga
      181 tcgggtaggc atagccgtct gcgggggcga gcttcacaga gtaatccgcg tccacctgcg
      241 tgttcgtttt gccggagaag gtgcagctgg taagatcgaa cgccacggtg tagacgccct
      301 caggggcgtc ggcccattgc gcggtaagcg tcgcatttcc gcggactctc aggctggagg
      361 caatcttctc accgccgctc catttggcaa agaccttgcc gtccggcggc gtaaaggtac
      421 actccggcgc acggaagtag gcatagggcg ttgcagatga cttttcatac tcataaccgt
      481 ccctgtgcgg gaaaaccagc atcggcgtct gcgtgccggt accgccgttg ggatcgaacg
      541 tcacggtata cgggtggatg cggggaatgg tcttgatgcg gatatcgccc gtgacatatt
      601 ttccggggat cgtgatctcg ccggtggcgc agttgagcgt gtagtttgcc gagttgagca
      661 cctcatcacc gatggtcaca taggtatagg aggtgtagct ccccatcgcc gcgtggtagg
      721 gcagctcata gccctcgtct gggacgagtc ttgcggagta atcctgtccc ttcttggcgt
      781 attttccgcc gatgatgctc gatctgatgg cggtgacgag aacggaatcc tcatccgcgg
      841 ccttttccca ctgcgcgtag accacggtat cgcnnnnnnn nnngccgtag aacttgttgg
      901 caaaatcaaa cggatcggaa tccgcctccg gcccgactgt ccagccggag aacgtgtaag
      961 cgctgtccac ggccttgggc gtcagtttcg gcatgcttac ggaatcgtcg tggaagagac
     1021 cggaggtaaa gcacagcacg tcggcgggac gggtctccgg cgtgaattcc tgcccgttgg
     1081 cgctgaaggt gacgtaaaaa agccttgtgg tcgtcgaata accggccgcg ctgcggttga
     1141 cagtcacggg acggatgtat ttcccgtcga tgtagaggtc gtactcgccc ggcttcgtgc
     1201 tctcacagac gtaatcggtg cagttcccgt cggtgccgga ggccttcagc gtatatacga
     1261 cgctgccggc cctgtcgcgg tactccacaa gggcgccggc ataaggcttg ccgcggagag
     1321 agaccttttc gtgcatatcg gcatactggt accacacggc gcgcagcgtc atgctgctct
     1381 ccacggtgtc tttggcaaaa ttccatgcgt ccgaggaggc gtcggaaaag aggtgcgccg
     1441 tccaatggac gaagtagtat ttgccgtcct cgcagcccgg cgccggggat tcgggcgcgg
     1501 tgatcttgct gcccttcggc acggtgacgc ttaccggagc cgcgccgccg tggccgccca
     1561 tatcgaacgt gacggtgacc atttcttcct cgccctccgc cgcggcggaa agcggcagca
     1621 gcgtgaagca gagcgtaagt acaagaagca ggctgaacag acgttttttc atatcattcc
     1681 ctctctctaa agctacggac agattgcggc ttcgcacaaa gtatatgcgc ggcttggcat
     1741 atacaccata tagatacaga ataccatata tgccgcccat ctgcaagaac aaaaaacggc
     1801 gcacggaaaa acatccgtgc gccggagaga aaattatccg aaacggtcaa agagcgcgtc
     1861 aacgacgccg gggctcgtgc ggtaatcaaa cttccgcgcg aagagcgccg tttccggtgc
     1921 ggcgagaagc tcatcgaggt cgccgtccgt gaatgtgtag ggattgccgc gctgccagtc
     1981 gatgtaccgg agacagctcg cgtagtcgtt ctcaaacgcg tgctccggca gcgtatcgcg
     2041 gaagggggag tttacgatct ccgtctggac gaacatttcg tccggacagt aggtatcgga
     2101 gaaaaactca cggatctcgc tctctttttt gagcagatac tgcgcgagcg cgtgcgtgat
     2161 gctgaaccac tgcgcgcctt tttggaaaac gaagccgtcc ggccgccgcc gcgtccggtc
     2221 tacgccgaag ccgagctgca gcgcggcgga gagattgcgc agcgtccggc gcacggcggg
     2281 ttttcggatg ttatagcgct gcagggggta gtaaaaccgg acgcggtaga gatccgtgcg
     2341 ggaaatctcc ggcgagagaa agcttacata gtttctcccg ggcgcacggg taaaatagtt
     2401 ttcaatgcac gcgcgcgttt tgactggcac gtccacgccg gagagaagat ggtagtagtc
     2461 atatccaccc ggcacagcgg cgcggagaag ctccagctcc gcctcgacta tggcaaaatg
     2521 gccccacgaa atgcggtggc gcggcacgaa cgtgacacgc gactttttcg gcacggcacg
     2581 aaaacgctcg ggatcgaatg ccgccttggc gtctgcatga atataaaaat cggcgttttc
     2641 gctgtcgaga aagcgcagct gcttttcgag gatctcaaaa ttcccatgtg tgagaatgag
     2701 ccatgcgtgt cgcatgaaaa acaccccttt cggcgctttc attatacgga gcatcggcgg
     2761 gaaagtcaat cggcacgccc gtgcagaaag ggagaagcgc attgacattc cacgctttac
     2821 aagataaaat ataagaggaa ttttatacac atgggaggaa cccccatatg tccaagcata
     2881 ccgataccgg ccgcgccggg acccgccgcg gcgggaaaaa gctgtggata ctgatcccgg
     2941 tgatcgccgt tctcatcatt gcggcggcgc tggtgtggct gaactcggtg tatgtttacg
     3001 ccggcggcct ccaccgccgc gacagcgcgc agatcgacgt gcgcggcaag agcatttcgg
     3061 aggaaaggta tcttcgtctg cgcgagcagc tgccggactg caaaatatac tgggacgttc
     3121 ccatcggcgg cgcagtgatc gactgcgaga gcacggccat catgctcacg tcgctcacgg
     3181 aggaggacgt cgcccgtctg gcatttttcc ccgcgctcgc ggagatcgat cttacggcgg
     3241 cggacgtttc tccggagcgc tttgatgccg tccgcgcggc gtatccggcg ctttcggtgc
     3301 gctggagcat ccccatcggc gcgagccggt atccctcgga tgctgaaagc atcacgctga
     3361 cggacttcac cgtttccgag ctgccgcttt ttgactactt cacggcgctg cgctctgccg
     3421 atgcgcgagg ctgcgcgtgc tacgacgctc ttctcgccct gcgggagaaa tacccggcgc
     3481 tcgaactgga gtggcaggtg ccgctcggca gcacggagta tcttgacagc gccacggaga
     3541 tcgccgtgga cgatatctcc atcacaccgg acgcgctgcg cgaggcgctg cggtatcttc
     3601 cggcggcgca gaccgtgtcg ttccccgcgt gcccgtggag cgagacagaa aagaacgcgc
     3661 tgcgcgccga gtttcccgcc gttgcgttcg tctggcccgt ggcgatcttc ggcgatactt
     3721 acccgagcga taccgcggag ctgagctttg ccggacgcac attttccgaa gcggatgttg
     3781 cggagctttc ggaaaagctc gcctcgnnnn nnnnnngccg aagctcacgc gcgtcgatct
     3841 caccggcacc ggcgtgacgg tcgaacagat gacgccggtc tgcgagaaat acccggcggt
     3901 cgattttgca ttcacgttcg agctttacgg cgtgccgatc agcaccgagg acacacttct
     3961 tgactttacc ggcatcgaga tggaatccac tgaaccggtg gagagcatcc ttcccgtgat
     4021 gcacaagctt gaaaaggtcg atatgtctga ctgcggcttt tccgatgagg aaatggacgc
     4081 gctgaataag aaatatgaga acgtgcgctt tgtgtggacg ctgcacataa cgcactacaa
     4141 catccgcaca gacaccacct atttccgcgc ctcctcccgc tattacggat acttcacgaa
     4201 cgaaacgatc aagaagctcg cttactgccc ggacatgatc gcgctcgatc tcggccaccg
     4261 gccaatcgag gatctgagct tcctctatca gatgcccgat ctaaaatatc ttgttctgct
     4321 cgactgccat gcactcgatc tctcaccgat cgcctcgtgc gacaatctca tctggttgga
     4381 gctcaaccgc gcttacgcca ccagcatcgc ccccctcaag gactgcaaga gcctgcgcga
     4441 tctgaacatc acgtttatga cgctccttca gccggaggac acgttccaga cgctgatgga
     4501 aatggacaaa atcgagcgcg tgtggttcag ctacggcgtt ctcacgcagg aggagcagga
     4561 aaaactgcag gaggcgcacc cggacatcgt atatcacggc gtgtacgact gggtacagtc
     4621 caacgaggac ccctggcggt gcgatcagga ttactacgac atgcgcgatg ctctcggcca
     4681 catgttctat atgaacggta ccggcatcat ccactgcaag atcatcgacg gtgtccggta
     4741 tccgcttgat ccggagtttg aggccacgat ggactgggga gagcacgaca gagaccgtta
     4801 aagacccttt cagacgtttt tctcgccgaa gcgtgggata catattgcgt tttatgccct
     4861 tatctggtac aatatacgga taagggcgtt tttatatcct ttcgacagaa tgggcggata
     4921 cgatgaaaaa gacttcccat gcgggacata ccgggcaggc agcactcagc gcgctgctgg
     4981 aaatatcgct ggcggtcgtg ccggcgctgc tgttcttcta tacatacgtt caccattacc
     5041 cgtcagcgac gttttttctg cggggcaatg tgctgttctt cgttgtctat tttgcgatcc
     5101 ttgtgctgtt catgacaatc tacggcagct ttagcgtacg gaaataccgc acgcgggagc
     5161 tggtgttcgc gttcggcctt tcaagctttt tcacggacac cattgcctat ttcctcatgt
     5221 gcatgatcgc aaaaatgctc ctgccaccga agtacgtcat cctcacgctg ctgttgcagt
     5281 gcctcgtgga gacagggatc tttattctcg cccgcattct ggccgaccgg ctnnnnnnnn
     5341 nnagccgccg gcgttggcgc tgctgatcgt gccggagaag gatgccgagg aaaacgtccg
     5401 cagcaagttt gaccgccgcc gcacgcgcta ctccatcgac cgcgtcgttt ccgcggatat
     5461 gccgtgggag gagctgtgcg agatcatgca ctcctacact acagtcgtgc tgatgccgat
     5521 ggaaaaggag ctgcgccgcc gcatcatgga cgactgcttc tggcacggca tcgccatcat
     5581 gctcaagccg gatatgcagg acatcatggt aaactgtgcc gacacggtca tcatgagcga
     5641 tgtgccgctc tgcgccgtgc acaccggcaa ctacgaccgc ggctatctga cggcgaagcg
     5701 cgtgttcgat atcgttgcgt ccgtatgcgg ccttatcatt tgcagcccga tcttccttgc
     5761 cgtcgccatt gccatcaaaa ttcaggacgg cgggccggta ttttaccggc agaagcgtct
     5821 cacgctgaac ggcaagccgt tttaccttac caagttccgc agcatggtcg tgaacgcgga
     5881 atccgccacc ggcgcggtac tggcagggaa gagcgattcc cgcattacga aggttggggc
     5941 attcctccgc gccacgcgca tcgacgagct gccgcagctt ctcaacattc tcaacggcga
     6001 tatgacgctc gtcggccctc gtccggaacg gccggagttt tatcagaaat actgcgccga
     6061 gtacccggag ttcgcctacc gcctgcgggc caaggccggt ctcaccggct acgcccagct
     6121 ctacggaaaa tacaacacca cttttgcgga caaggcgctg ctcgatatgt actatatcca
     6181 gaaggcgtcc tttctctggg atctgcagct cattttctat acgctgaaga tcatcttcgt
     6241 caaggagagc accgagggcg tggacaccaa acccgccgag ccggagaaga aagaaccggc
     6301 ctgcgccgga gaaaccaaat aattttgctg ctctttcgct aaaaaaccgc tgcgaatttc
     6361 ctgctccata taggaggaaa attcgcggcg gttttcgttt tgcgcgtgtc ttgatgtatc
     6421 ggcagaggcg gcgcgtcaat cgtatgcgac ggtatagttc agttccccca aagaagcgag
     6481 ccggatcgtt ctcacctcaa aggcggcata ggtaaaggtc atttcttcgc ctgcgggaag
     6541 agctgtttca tcggagccaa tcgtaatgtg caggggggcc tctccgttat tgcggattgt
     6601 caccgatgtg aatttgtgtg tttcaaagtt tgttttgata acctgtaagt gtaagccgct
     6661 gtcgctgaaa aacagctcgc cgacggcagc aatatcaccg ctgtcatgcg ccctttgaac
     6721 gacacacaga ctgccgtccg atccatccag ccggagggag tagtcatatt ttccccggtc
     6781 ttccagcgtg taactcgcac cgttcgcgta gtcgtaaacg atgacgcggc tatcgatgat
     6841 accggagccg aaggtgatcg tggaacacag ctcgggaagc ccatcgccgg taaggtcgca
     6901 gaaatacgtg ttccagatcg gcataccggt ataaagcggt gtgatctcgt tttccgtcac
     6961 agcctccatc ttctccggat accagcggaa cgtcacgccc ggatattccg ggagagcgat
     7021 ctccagacgt ccgtcccagt tcatttcctc ggggttttcc aggtagtcga accacttttc
     7081 cgtttgcgcc ggtcctaaac cggacacatt atcaccgacg ctgggcgagc tccgctctgg
     7141 tttcggatcc gtcagaaagc agacggcggc cacggcgcag acaaccacag acgccagaac
     7201 gatccagaat gcgggcttct tatagttcat cacggacttc acccgctcct ttatgcccac
     7261 ctcgccgaaa gcgaggggac acgccgcgat gctgcggcgg ctgacgctgc aggatacaag
     7321 cgcatgcgta taatcggctc tttgttcatt gcccatttcc cggatgaatc tttcgtcgca
     7381 ggcaagctct atatcccggc acagcaggat atagccgagc catagcagcg gattgaacca
     7441 gtggaccgtc agcagcagaa aagcgagcgg cttccaccag tgatccccgc ggcggatatg
     7501 cgcctgctcg tgggcgatta catacgccag agcgccgctg tccacggaat acggaagata
     7561 gatttttggc tggatgatcc ccagtacgaa cggagagccg gcattttcgc tctgatagat
     7621 attatcccgc aaaatcacgg cttcgcggac tctgcggcgc agacgccaat agcttatggc
     7681 ggaataaagg agaagcgcgg cggctccggc gagccagatc gccgccatga ccgagatcca
     7741 tatctgcagc ggattggcgc tcgcgcccgg cgcgggcgaa agcgaatggt caatgaccgg
     7801 gttgatgacc tgatcgaggg cgggaacgcc cgtttgaacg gaaggcgccg tctccatcat
     7861 aatgctcggg ctgaccgttt ccgcgctcgg tataagactc agcgcactct cgatggaaaa
     7921 cgggaagatt aaccggacgg cgacaatgcc ccagagcaga acattcaccc attttgacgc
     7981 ttttttcagg cagaaccgca atatcagaac cgcgatcacg atccagcttg cggctatgct
     8041 ccggttgacg atctccagaa acagctcgct cacgatctct ccccctttcg gatacggtcg
     8101 atcatctgct gcacctcgtc gagctcatca tcggagagat cctgacgtct ggtaaaggcg
     8161 gcgataaacg cggggagaga gccttcaaac ttcttttcca ccagctcgtc gatctcccac
     8221 gcctgcgcct cgtcttttga tacaagcgcc gttaccatgc cgtcttcatt tttcaggacg
     8281 ccgcgctccc caagccgctt gatgactgta tacgtcgtgg tccgcttcca tcccagctgc
     8341 tcctgacaca gccttgcaag ctgcgcggcg gcgatcggtc cgtgctccca caaaatcagg
     8401 cagaaacggt attcgccctc gtgaatcttc ggaatctcca tgggtaagcc ctccttgtct
     8461 acgttgatag actctatgca tagtctacac ggttagacaa cggaagtcaa ggaaaaatat
     8521 aagaacacac aaggtatcag ctgctcatat taaggattgc attttccgtg gtttattggt
     8581 aaaatacctt gataaactat acagatcatc ggaaggaaca gggaaatggg cgaacggagt
     8641 ctcatcagtg tggtcgtgcc ggcgtggcgc gccgaaaaaa cgcttgccgc cacgctcgat
     8701 tccatcctct cgcagacatg gcgtgagctc gaggtgctcg tcgtggacga tgcctcgccg
     8761 gacggaacgc tcgctctggc acagagctat gcggcgaaag agccgcgcgt ccgtgtgatc
     8821 gcgcagtcgg agaatggcgg cgtatcgaaa gcgcgcaacc gcggtgtgcg cgaggcgcgg
     8881 ggcgaatgga tcgcctttct cgacagcgac gatctctgga tgcccgaaaa gctcgagcgc
     8941 cagatggcgc tcgcggcaca gcacccggaa gcgggcctct ttttcacggg ctcggcgttc
     9001 atttctgaag acgatcacaa atacggctac acgctctccg tgccggggcg cgtgaattac
     9061 cgccgtctgc tcgggcagaa tgttatttcc tgctcgtcag tgctcgtccg ccgggaggtg
     9121 atggagagat tccccatggg gaacgacgcc atccacgagg atttcgccgt ctggctgaag
     9181 atcctccgcg aagagccatg ggcctacggc gtaaacgagc cgctgctcgt ttaccgcgtg
     9241 tcgcgctcgt ccaaatccgg caacaagctc aaggctgcgc gcatgacgtt tggaacgtat
     9301 cgctacgtcc gcgtcccgct gccgctcgcg gtctggagcg gcatgctgta taccgtccgc
     9361 ggcatccgga aattccgcgc catcggcgcg tcgctgacgg acgatacctt tgcgcttccc
     9421 gcgccggaag ccggatcgga gacggaatga agcgagtctg ccatctcacc agcgtgcaca
     9481 gcagcgacga tacgcgggtg tttcataagg agtgcgtgtc gctcgcgcgc gccgggtacg
     9541 atgtaacgct cgtcgccccc ggcgagagcc gggaagagct cggcgtgcac atcgtcggcg
     9601 tgggggaaaa gcccgtcggc acgcttaacc ggctcacgtc gcccttcgca aagcatgcct
     9661 acgaagcggc gctcgcgctc gactgcgagc tctaccatct gcacgatccg gagctgctgc
     9721 cgtatgcgct caagctcaag cggcacggga aaaccgtggt tttcgacagt cacgaatttt
     9781 attccctgct cattcaggaa aaggggtata tccccggctt tctcaaaaaa ccggcgtcgc
     9841 gcatttacgg cgcgctggaa gcgggcgttt tcaaaaagct cgacgcggtc atcattccct
     9901 gcacctcgtt cgggaaaaac ccgtttgaga agtgcgccaa gcgcactgtg atcgtggata
     9961 atctcccgga cgccggacgc ttcccgccac cggaaaagct ctatccggac gcggagaaca
    10021 cagtcggcta tgtcggcggc gtgactgccg agcgcggcat tacaaatctc atccgcgcat
    10081 gcgccatcgc cggggcgcgg ctgcatctgg ccggttccgt ctggccggcg tatctcgaag
    10141 agctgaagaa aatgccggag tttgcgtgcg tggactatgc tggtgtcgtt ccgtatgccg
    10201 aggtgccgga atactgccgc catttctcca ttggtatggc gacgcttttg aacatccatc
    10261 agtacatcgg caccgacaat ctccccacca aggtctatga atacatgggc gcggggcttc
    10321 cgtccatcgt ttccgacacg ccatcggcga gggaactgga aaaagatttc ggctgctgcg
    10381 tgtgcgttga cccggacaag ccggagaaaa tcgccgccgc catccgctct ctgctggacg
    10441 atccggagcg tcgggcggct atgggcgagc gcggctacag tgcgctgcaa agccgcttca
    10501 actggcagac cgaggagaaa aagctcctgg cgctgtatga agagttgctg ccgctcaaag
    10561 gagaataaac catgctggaa gaacccatat accgccgcat aaaagaagaa tacggctttc
    10621 ccgtctatat ttttgattcc gaccgcttct gcgagaatta ccggctgctc accgggggca
    10681 tgagcgccct ctacgcgccc taccgtgcgg cctattccta caaaaccaac tacacgcccc
    10741 gcatcgtttc actggtgcgc tcgctcggcg gcatggcgga agttgtctcc ggcatggagt
    10801 acgctctggc gcggcgcgtc ggcaacgcac cggagaatat cctcttcaac ggcccgtgca
    10861 agggcgagga cggaattgcc gcgctgctcg acggcgcatg cgtcatcatc gataatgagg
    10921 aggaactcgc cgcggtgctc cgcgcggcat ccgctcaccc ggaatgcgtc tgtcatacgg
    10981 cgctgcggct gcatgtcgat atcggcagcg gcaagccctc gcgtttcggt ctgtgcgccg
    11041 aaccggaata tctgaaaacg ctcgccgcgc ggctgcgcgc ggcggggaac atctcctttg
    11101 acggcatcca gtgccacatc agcggcagcc gcagcattgc cggatgggag agccgcgcga
    11161 gagtcatgct cgcgctcgca cgggagcttt tcccggatgc accgccgaaa tttattgatc
    11221 ttggcagcgg tatgttcggc cgtctgccgg aggaaatgcg cgcttcgttc gctcagagta
    11281 tcccgacgtt tgccgagtac ggcgcggcgg tcggtacgct tttccgggcg cagtacggcg
    11341 cgctgccgat aagcgaacgc cccgcgctca tcacggagcc gggtacaacg ctcgtggccg
    11401 acacgatgca gtttgccgcg cccgtaacgg cgatcaaatc cctgccgggg aacgactgcg
    11461 cagtgctcga tgcgagcatc cacaccgccg gcgtcatctc gcagatgcga aatctcccgc
    11521 tgctcgtgct cgacgcgaaa acgctgcggg aaaatcttaa tttcaccggc tacacctgtc
    11581 tcgaatacga catcctgtac cggggctaca ccgggccgct cgatgttggc agcagcgtga
    11641 tctttgataa catcggctcc tattccaacg ttctcaagcc gccgtttatc catcctgatg
    11701 ttcccatggt ggaataccgg cggagcgatg atttgctgca ccttatcaag cgggcgcaga
    11761 cggcggagga aatcttcgcg cagtatgttt ttgaggagta aaagagatat ggatgaatgt
    11821 acagtactca tgaccggctg cggcgcgccg ggcgcgccgg ggatcatccg gtgcctgcgc
    11881 aaaaacggtg agcgaaacat ccgcatcgtc tccgtggacc gcaacgagaa tgccggttcg
    11941 cgcgatctgg ttgatgcgtt ttataccgtc ccctcggcgg agaaggaaga tttcattccc
    12001 gccgtactgg atatctgccg ccgggaaggc gtgcaggttg tcatccccat cgtgacgcgc
    12061 gagctcatga agttctcctg cgcgcgggcg gagtttgagg cccacggcgc gaaggtatcc
    12121 gtgatggagc cggacaagct ggagatcgtt aataacaagg cacatctgct cgatgcaatg
    12181 cgcgcgcatg gcctgcgcac gccggagtac aagcgcgtgc ataccgtgga gaagctgaag
    12241 gccgccgtct ccgcgctggg ctatccggaa aaggcgctgt gcgtcaaggc ggcggtcggc
    12301 aacggcagcc gcggcatccg catgctcaac gcgaatattt cgcgctttga ccttttcttt
    12361 gagcagaagc cgaactccat gttcatcagc tatgaggagc tgatccgcac gctctccgag
    12421 cgggagacca tgccggaaat gctcgttatg gaatacctcc ccggcaccga atacagcgtt
    12481 gattttcttg ccgaccatgg tgagccgatc tatatggtat cgcgccgcgg cctctcggtc
    12541 gtcacgagca acatgatgtc gcttgtcgtg gacgacaatc cggcagtcaa ggaaatctgc
    12601 acgcaggtcg tgcgctctct tgagctcgac ggcaatttcg gctttgatct tctctataat
    12661 gccaacgatg aaacgccata tgttatcgag atcaatccgc gcctgaccgc gggcgttgtt
    12721 tcgtgtgcgg cggcgggtgt caacctgccg tatctcggta tgaagcggtt gctcggcgag
    12781 ccgctgccgg agctgacgcc tcatttcggc acgcgtatgt cccggcggta tcaggaggcg
    12841 tttttcgacg ccgccggaaa ccgacttgac tggtaaagga gctgacgata tgaagaaagt
    12901 tctagtcctc gccccgcaca ccgacgacgg cgagctcggc tgcggcggaa cggtggcacg
    12961 gctgctggag gaaggctgcg aggtgcattt cgctgtgttc tccacctgcg cggagtccgt
    13021 cccggcgggc tttccggcgg acgagctgaa aacggagttt ctctccgcca tggagagcta
    13081 cggaataccg gcggaacagc ttatcctttt cgattttcag gttcgtcatt tcccggcgca
    13141 ccggcaggag attttggaag agctcgtgcg gctcaatcgg cgcatcgtgc cggatctcgt
    13201 gcttgccccg tcgctgcacg acgtgcatca ggatcaccat accatcgccg aggaggccat
    13261 ccgggccttc aagcgcacga gcatcctcgg ctacgaggag ccgtggaaca atctgacgtt
    13321 caacaatcag gtatatgtta cgcttgaaga gcaccatgta gaaaagaaga tcgccgctgt
    13381 ggagcggtat atctcgcagc gtgggcgcat ttatgcctcc ggcgagtata tccgcgcact
    13441 ggccatgagc cacggcgtgc agatcggacg gcagtacgcc gaggtctttg agaccgagcg
    13501 ctggatcctc tgacaagcac agacatccgg cgcgcaaaag gaaaggaacg ccatgaaaaa
    13561 gatcctgctg gttttcggca cgcggccgga ggccgtgaaa atgtgcccgc tcgtgcagga
    13621 gctgcgcgcc cggcgcgaaa taaaaaccgt tgtctgcgtc acgggacagc accgcgagat
    13681 gctcgaccag atccttgcaa cgttttccgt cgtgccggac tacgatctct ccgttatgaa
    13741 ggattcgcag acgctctttg acgtaacgac cggcgttctc gaagggctca agggcgtgct
    13801 ggaagcggaa aagccggaca tcgtcctcgt gcagggcgac acgacgaccg cctttgccgc
    13861 cgcgctcgcg gcctactatc tgcgcatccc ggtggggcat gtggaggccg ggctgcgcac
    13921 gcgggatatc tacagcccct atcctgagga attcaaccgg caggcggtgt ccatcgtcag
    13981 ccgctacaac tttgcgccca cggagcaggc aaaggaaaat cttctcgccg aggggcgcga
    14041 ccccggcacg atccacgtga ccggcaacac ggtcatcgac gcgctgaaaa cgaccgtccg
    14101 tgccgactat acccatccgg agctcgcatg ggcggcaggg aagaggctca ttctcatcac
    14161 ggcacaccgc cgggaaaatc tcggcgagcc gatgcgccac atgttccgcg ccatccgccg
    14221 cgtgctcgat gagcacgacg atgtccgcgc gatctacccc atccaccgca accccgccgt
    14281 gcgcgaaacg gcggcggagg tgttcggcgg cgacgaacga atccatatca tcgagccgct
    14341 ggatgtgctg gatttccaca actttctcgc gcgcagctat ctcgtgctca ccgacagcgg
    14401 cggcattcag gaggaagcgc cgagcctcgg caagccggtg ctcgtcatgc gcgatacgac
    14461 cgagcggccc gagggcgtcg cggccggaac gctccggctg gtaggcacac gggaggaaac
    14521 gatctacgag agcttcacgg agcttttgga taacgcggcg gcgtacgaaa aaatgagccg
    14581 taccgcgaac ccctacggcg acggccgcgc ctgccgccgc attgcggaca ttcttgcagg
    14641 agaataacgg atgaaaatcc tcattgcagc ctctggcttt gacagtccga gaaaacacga
    14701 agtaaatatt tttgctctag atcaggccaa agcgctgcgg gatgccggac acgatgtccg
    14761 ctttgccgcc gtggacacgc ggtcgatccg ccactcccgc ccgtgggggt gttatcgata
    14821 cacgctggac tgcatacccg tttattacgc ttcggttccc tgcggcgcgc tgccgctggg
    14881 gcttccggag ctggccggac gggtagcggc ctccggcatc cgtcgcgcca tagcaaagga
    14941 tggttggaag ccggacatta tccaccagca tttcggcttt gaacttgccg cgatggcgga
    15001 gcgggagggc attccgttcg ttttcactga gcactcctcc ctgcacaacc gtgcgctttc
    15061 accggagaag gcggaacatc tgaaaagaaa atatgagtgc tgtgccgccg tgctggcggt
    15121 cagccaggcg cttgcgaaaa acatgcgcgc caacaccggg gtcgaagcta ttgtcgtacc
    15181 caatatcgtg gatacggcgc ggtttgcgcc gaagaggatc gcgcacgagc gcttcacgtt
    15241 cgtatcagct ggcaatctca tcccggtgaa aaacatggcg gggcttctcc atgcgttcgc
    15301 tgcgctgcac ggcgagccga ggcttatcat tttcggcgat ggaccggaga gcggcgcgct
    15361 gcgggcttta tgcgccgaac tggggctgta cagccgcgtc tccttccgcg gccactgtcc
    15421 gcgagaggaa cttgccgaag catacgccgc ggcggactgc tttgcgctgg cgtcgaggag
    15481 cgaaacgttt ggcgtcgcct atattgaggc aatggcggcg gggcttccag tgatcgccac
    15541 gcgctgcggc ggtccggagg attttgtcac ggaggagaat ggcatcctga tccctgtgga
    15601 cgacgagagc gctctcactg gcgccatgga gcgcatgatg ctccgccgcg aagactacga
    15661 cagcgcggcg atctccgcgg acatccgccg gaaattcacc cccgagacca tcgccgcgcg
    15721 gcttaccgag ctttatgagg gagttctttc atgctgattt tgtatatcac ctatgtggat
    15781 attaactccg gcacctccgg atcgtcggta cgcccgcaga agatgtaccg ggcgtttctc
    15841 gacgagggac acgaggtaaa gcgcctttcc ggcacacagc agcgctgggg aagaaaggcg
    15901 cgcaccgaag cggtacagga gatacggcgg tggcttaggg aaaaccggcc ggatatctgc
    15961 tatatcgaat ctccctctat gccgatcacg ttccgctgcg atatcgctct tatccggaaa
    16021 atctcccgaa tgggaattcc catcgggtat ttctaccggg atttctattg gatgtttccg
    16081 gaactgtatc cccgccggac agatattacc ggctggctga aggatcactg gctggattat
    16141 ttgcagcagc gcacaaataa cgtgctcaaa tgtgcagaca tcgtgtactt tccatcggaa
    16201 gaatgtaaga agtatttttc ctatacagat atgcgttcgc tgcccccagc aggcgaagat
    16261 catctttctg agcggtgccc ggaggaaaag acctgcatat atgtcggtgg gctggacggc
    16321 gggtacggcg tcgggatgct gcttgaggcg cttgagcagc taaacgccgg agacaggaca
    16381 taccgtctga tccttgtttg ccgggaaaag gaatggaaag cgtttcaaag tccgtataag
    16441 gaagctccgt ggctggaggt gcatcatacg tcgggcgagg ggctgaagcc gctgtatgca
    16501 cgcgctgccg tttcggtcat accggtgtct ccgacaccat acacgcattt agcggtcaat
    16561 gtaaaacttt ttgactacct gagttatggt ctgcctgttg ttgttacaaa cgctcggacg
    16621 atttcggcca ttgtccgtga aaacggcatc ggtttggtcg tgccgtatga ggcggaagct
    16681 ctggcgaacg cgctgcaaag cataatggac aatccaggga aaagaaaaga atatgaaaaa
    16741 caatgtatcg aagcactgag aaacggaaat ctctgggttc accgcgttcg tcagatcgtt
    16801 tcggatttgg aggaaaaata atgaagaatc ttcttcttct tacgtttgcc ggtctcggag
    16861 agcggcataa tgttggcgcc cctacgttct ggaaaacggt agaggggttt attgctgccg
    16921 gatggaaggt ttggattgtg gatgtcggca caaaagagga cacgccgctc ggcgaacggg
    16981 actacgggga tggactttat atcgtgcggt ttaatcctcc ttttttgagg gagaccagaa
    17041 tccggaaaat tggatggttt tttggctttg ccaatgtgtt tgccattcgc catatcctga
    17101 tccgggaagc agggcgactt ataaatgaac agcatcttgc cgccgataat actgtggttt
    17161 acggacagga cacccacgca gtgcacgccg cgaaacaaat cagcaggacc tacagtatgc
    17221 cgttggtcac gcggttttat ggcatatggg acatgatgac gaaaaaagat aatttagtca
    17281 accgtctccg gtattacccc aagctgcagg catacggtgt cgaggcggac atggtgctga
    17341 tgacaaacga cggtacgcgc ggagatgaag ttttgaagcg ctccggaaat aagagtagaa
    17401 agattttttt ctggcgcaac ggcgtagata ttccgggagc gccggctgtc tctctgggag
    17461 cggagcttcc attgaataaa tcggatacag tattaatgac tctctcccgc ctgcaggcgg
    17521 gaaaacgggt gaatctggca attgatgcac tcgcagaggt tgtaaagtct catccggagg
    17581 caaagcttgt catcgttggt tatggggaag agagaaaaaa tctggaagaa caggtatgcg
    17641 cgctagggct ggaaaagcat gtgattttca cgggaaaaat atcccatgag ttggtctatg
    17701 actatttgca gcgcgcggat gtgttcattt cattgtattc tgcatcaaat ctcggtaatc
    17761 cgctttttga ggcgatgcgc tgcggaaaag cgatcatcac cgttgacact gggacgaccg
    17821 gttcagtaat caaaaacgaa gcaaacggta tacttcttcc ggaaaaagag ctttcctgtc
    17881 tgcccgaaga gatatgtcgt ctgctgtcgg acgaagatga acggaaacgt ctcggtgagg
    17941 gggcaaagcg ctttgctgac gagaattttt ggacgtggga cgagcgcatc gccgcggaga
    18001 tccgcgaggt aacggcgctt ctgccgccgg agaggaactg aaaatgcaca ttctggtatt
    18061 gccgtcgtgg tatccgacaa aggcggaccc tgttaagggg agctttttcg ctgaacaggc
    18121 ggcggcgctc gcgcgttacg gtcacacggt cacggtcatg gcggtctata acgacggcga
    18181 gcgtggagtg cagaccgaaa agcgctcaga cggcaatctt acggaatacc tgatccatgt
    18241 aaagccgctg cggttccatc ttacatattt tcgcattctg cgcgaaatgc tccgcattct
    18301 ccgtgagagc ggaaagccgg atatcattca tgttcactcg ttccgcgcca tccggtatgc
    18361 ccgtgcgctc aagcggcggc tgcatgtgcc gattgttgtt acggagcatg tcacgtggtt
    18421 tgaacggaat atgctcagcg aaaaggagct tgccgcggtc tcccgcgatt ataatgc
//
LOCUS       DAWAAE010000024        17913 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1426_length_17913_cov_3.340240, whole genome shotgun sequence.
ACCESSION   DAWAAE010000024 DAWAAE010000000
VERSION     DAWAAE010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 17913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17913
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1426_length_17913_cov_3.340240"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1610..1619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8939..8948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11381..11390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3533 a         5445 c         5407 g         3498 t
ORIGIN      
        1 cgaggaagcc gaccttgccg agctttttcg cctcgtcctc aaaaacgaga gcaaactcgc
       61 gcccgatgat cttgcgcttc tgctcgggat cggtcacacc cacgagcttt gcaagaaagc
      121 ggtcggcagc gtccacgcag cggaagtcga gcgccttatt ctcgaatacg gagcggatct
      181 cctccgtttc gccctggcgc atgagaccgt gatcgacata aatgcacgtg agctggccgg
      241 gcaccgcgcg cgcgagcagc gccgcgcaca ccgaggaatc cacgccgccg gagagaccga
      301 gcagcacctt ttcactgccg acctgctcgc ggatctcgcg gatcttcgtt tcaaggaagc
      361 tctccatcga gtaatccccg gcgcagccgc agatatcaaa gaggaagttt ttgagaatga
      421 gattgccctg cacggtgttg gtcacttccg gatggaactg cacgccgtag agccggcgct
      481 cttcgttcac atacgccgct acgggcgtga ggtcggtgtg accggcaacg cggaagccct
      541 cgggcagcgc cgtgacgaga tcggtgtggc tcataagcac gtccgtcggc acggtgcagc
      601 cggaaaagag cgggagaaag gtgtcgagag taatggggaa tacgccgtac tcgcgcttcg
      661 tgcccgcctc gaccgttccg ccgcagagat ggcagatgag ctgcatgccg tagcagatgc
      721 cgagcaccgg gatgccgagc tccagcacgg ccttgtcgca ggtaggggaa tcgtcgcggt
      781 atacgctgtc cggtccgccg gtgaagatga tgcccttcgg cgccatcgcg cggatctcct
      841 ccacgggcat tttgcacgaa cggatctcgg aatagacgcc gtgctcgcgc acgcggcgcg
      901 cgatcagctc cttgtactgc ccgccgaaat ccagaatcaa tacagtctct ccggtcataa
      961 atagcctccg cagcaaaaat ttttaaaaat ataaaggata tccgtcagca acgcatgatt
     1021 cggcaagagc gggcggcgca caagcgccgc cgagcgccga agacgatatt atgcggcgct
     1081 gaccaaacga aaaaagcgtc gccgacgcgg tcaggtaaac cgcgtcggcg aacgccattg
     1141 ctcaccttta cgcacacgaa ttatacggaa cggagccgtc tttgtcaata aaaaatcgcg
     1201 ggcaatattg acaaaatcat tggcacaggg tagagtgagc aagttaaagt gataaagaca
     1261 gggacacccc ctccgtcttt tgcctttggc aaaagccacc tcccctgtta gaggaggcaa
     1321 gatcgttttc tgcaaaccca cttggctccc cacgataggg gagactgcac ggcgcggccg
     1381 tgaccgaggg ggtcttcacc cggatatcta ctatatatta taaaaggggc agggcttatg
     1441 agcggtatga cagaagaatt cggctcgatg gtattttcgg atgccgttat gcagcagcgt
     1501 ctcccggcgg agatctacag cgcgctgcgc tcggcccggc agtatggcaa gcgtctgacg
     1561 ctcgccgagg cggacgtcgt cgccgaggcg atgcgcgact gggcgatcgn nnnnnnnnnc
     1621 cgcgagctca tccacagcga tccggacgcc tcgagcttcc cgtccggcgg acttcgcgcc
     1681 acattcgagg cgcgcggcta caccgcctgg gacccgacga gctacgcttt cgtgaaggac
     1741 ggcgtgctct gcatcccgac ggtattcgcc tcctacggcg gcgaggccat cgataagaaa
     1801 acgccgcttc tccgcagtat ggacgcgctg aatatccagg cgctgcgcat tctgaagctc
     1861 ttcggcgaca cgcagaccgc cgccgtgcgc gcgaacgccg gcgcggagca ggagtatttc
     1921 ctcattgacc gcggcgttta cgagcagcgc cccgatctta tctacaccgg gcgtacgctt
     1981 ctcggcgctc gcccgcccaa gggacaggag ctggacgacc attatttcgg cgcgatcaag
     2041 ccgcgcgtgg cggcgttcat gcgcgagctg gacgaggagc tctggaggct cggcgtcccg
     2101 gccaagaccg agcacaacga ggtcgccccc gcgcagcacg agctcgcgtg cgtctattcc
     2161 acggtgaaca tcgccgccga tcacaaccag ctcgtcatgg agtgcatgaa gcgcgtggcg
     2221 gaccggcacg gcatggtgtg cctgctgcac gaaaagccgt ttgcgggcgt gaacggctcg
     2281 ggcaagcaca acaactggtc gctttccacc gatcgcggcg aaaatcttct gaaccccggc
     2341 gacacgccgg aggaaaacgc gcagttcctg ctcatcctca cggcggttct gcaggccgtg
     2401 gacgaatacc aggacctgct gcgcatctcc gtcgcgggag cttccaacga ccaccgtctc
     2461 ggcggcagcg aagcgccccc ggcgatcctc tccgtgttcc tcggcgacga gatcaccggc
     2521 attctcgatt ccattgagaa cggcacgccg tatgaatccg gcgaaaaggt gcagttcaag
     2581 gtcggcgtgc atgtcctgcc gcgcttcccg aaggatacga gcgaccgcaa ccgcacctcg
     2641 ccctttgcct tcaccggcaa taagtttgaa ttccgcatgg tcggctcgtc gcagtcgatc
     2701 tccgaggcga acatcattct caacaccgcc gtggcggacg tgctgcgccg ctatgccgac
     2761 gagctggagg gcacggcgga ctttgagagc gcgctgcacg agctcataaa gcgctccatc
     2821 cgtgagcacc gccgcatcat cttcaacggc aacggctacg aagaagcatg ggtgcgcgag
     2881 gccgcgcgcc gcggcctgtg caactaccgc tccacgccgg actgcgtacc gcatctgctc
     2941 gacgcgaaaa acgcggagct tttccgccgc aaccgcgtgt tcagcgaaac ggagcttcgc
     3001 gcccggtgcg agatcgtgct cgaccactac gccaaggagg tgcgcatcga ggcgctcacg
     3061 ctgctcgata tgctgcgcaa ggatgtgctc cccgcggtga gccgctttac cggcgctctt
     3121 tccgccacgg cgcacgaaaa gaaaaagctc tgcggcgaga gcgcctgccg gtatgaaaaa
     3181 acggcggcgg cggagctctc cgcgcgcgcg gcgaagctct gggacggcgc ggaaaaactg
     3241 gaaaaggcgg tgcgccgcat tgacgacgga cgcctctcca ccgaatccgc cgtggagatc
     3301 tgccgcggcg agatattgcc ggaaatggag acgctgcgcg aaatttccga cggcgcggag
     3361 ctcatcatgg accgcaccgc gtggccgtat ccgtcttatg gtgagatcct taccagcgta
     3421 tattaaaatt tagaagaaaa ccggcgcttt ttggtaaaat accgaaagcg ccggttttgc
     3481 cgccttgaca tccggcgaag aaaccgatat agtatggcca caaacaaaca gcgtgtggat
     3541 ccgcgtcgaa agacgtcgtc ggaggagact ccgccgaggg aacacggatg caaattccgg
     3601 actatcccag taaccgtaaa gcggacaaaa aagccggtac cactgccgaa gggcgggaag
     3661 gggcttgagt aggatgatgc cagtcgggaa acctgttttc gcatgagagc ttctgggacg
     3721 gggtatagct gtgcgatcca tcgccgattc cgccgccctt tccgagagag gggcggcgtt
     3781 tttgcgccgg aaggctggtt gtttgaatac ttttttgaga aggagttcaa gaaatgaacg
     3841 ccaaaaagat ccttgctctt gcgttggcag ccctgatgct gctctcgctc gccgcctgcg
     3901 gacagagcgc tgagcccatc gcgacggaag ctcctgccga gccgaccgag gcccccgtgg
     3961 aagccaccga agcccccgcg gaggacacct ccgtcaccgt gaccgacatg accggccgcg
     4021 aggtcacgct cgaagagcct gccacgcgca tcgttgcgct cacggcgtcc gactgcgaga
     4081 tcatctgcgc gctcggcggc atggacctgc tcgtcggccg cggcacctac tgcgactacc
     4141 ccgccgaagt gcaggagatc ccctccgtgc agtccggcta tgagaccaac atcgagcaga
     4201 tcatcgctct tgagccgcag gtgctcctta tgagcacgat ggcccagacc gaggagcaga
     4261 tcaaggcgct tgaggacgcc ggcgtccacg ttgtcgtttc cgacgcgcag gatatcgagg
     4321 gcacctatgc cgccatccgc atgatcggcg cgctgctcgg caagaacgag gaagccgaaa
     4381 cggtcatcga cggcatgaag acgacgtttg agtcgctcaa gggcgtctgc ggcgacggcg
     4441 aaaagaccat ttactttgag gtctccccgc tggagtacgg tctgtggacg gccggcgcca
     4501 acaccttcat gaacgagatc gccgagctgc tcggtctcaa gaactgcttt gccgacgtgg
     4561 aaggctgggc ggagatcagc gaagagcagg tgctcgagcg caaccccgac tatattatga
     4621 ccatttccat gtactccggc gagggcccca cgcccgaaga ggagatcgct tcccgcgccg
     4681 gttgggaaaa tgtgacggct gtgaagaacg gcgccatcct caatctgcag aacaacgagc
     4741 tttcccgccc cgcaccgcgt ctggcggaag gcgcgcagat gctcgccgac ttcattgccg
     4801 gcgttgaggc caatgacaag gcggcctgag ccaaaagaaa ataaaaccat ggtacgccgg
     4861 gaggggtttg gccttccggc gtatctggca tttgccgcgg cgacggtatt cacgcttctc
     4921 gtgtgcgtgt cgctcggcag cgtgaagctg ccgctgcgcg agacgttcgg ccttttgtgg
     4981 aacgccatgg cgggccgcgg cacgggaacg ggcatgagcg cgacgatcgt attctccgtg
     5041 cgcctgccgc gcgtgctgtg tgttgcgctc acgggcgcgt cgctctcggt gtgcggcgcg
     5101 gcgatgcagg gccttttgaa aaacccgctg gcggacggct ctacgctcgg cgtatcgtcc
     5161 ggcgcgtcgc tcggcgcggt gatctccatc gcgttcggca taacgctccc cggcatcccg
     5221 ctcgccggga cgatgagcat ggcaatgctt tttgcgttcc tctcgctcgt cgtcattctg
     5281 ggtcttgcgt accggctgga cggctcgctc tccaccggca cgatcatcct catcggcgtc
     5341 atttactcga tgttcgtttc gagcgttctc tcgctcgtca tcacgtttgc gccggataag
     5401 gtgcagtcca tcacgttttg gacgatgggc tcgctcgcgg ggcggggcta tatccacgtc
     5461 cttgtgcttc tcggcgcgct cgtcgttttc ggcggcgtgc tcttcggctg cgtgcgcgag
     5521 ctcaacgcct ttgccgtcgg cgaggacaac gcccggcaca tcggcgtgaa cgtccggcgc
     5581 gttaagctca cggtgatgat cgcggtgtcg gcgctcatcg gcgtgtgcgt gtccgtcggc
     5641 ggcacgatcg gctttgtggg tcttgtgacg ccgcacatgg tgcgcctcat caccgggccg
     5701 aaccaccggc ggctgctccc ggcgagcgcg ttcggcgggg cgattttcct catgctgtgc
     5761 gatcttctcg cgcgcacgct ctttgacccg cgcgagctgc ccgtgggcgt tgtcacatcg
     5821 ttcatcggct cgatcctctt cgttgtcatc ttcatccgca caaggagggg cgagaaatga
     5881 gcatgctgga agtacaaaac ctctcggtgc gcttcggcag cgccgtgatc gtggacgatg
     5941 tgagcttttc cgtgcaagac ggcgactggc tcatgctcat cgggccgaac ggcgcgggca
     6001 aatccacgat cgtgaatgcc gtgtcccgcg gcgtgccgta tacgggaacg gtgctttttg
     6061 aagggaagga cgtgcagaaa acgcccgccc atctccttgc gcgcggcatg ggcgtgctcg
     6121 cgcagcacca cacggtcggc tatgccttct ccgtggagga ggtcgtgcgg ctcggacgct
     6181 atgcctatgc tcccggtatt ttctcccgcc ggagcgacga ggacgagcgg agcgtcgccg
     6241 aggcgatgga gctgacaggc gtggcgcata tcgcgcgcca gtctgtgctc acgctctcgg
     6301 gcggcgagct gcagcgcgtc tttctcgcgc agctctttgc gcagaacccg cggctgctcc
     6361 tgcttgatga gccgacgaat catcttgatc ttgtgtatca aaagcagatc ttttcccttg
     6421 tgcgcgagtg gctctccaag ggcggccgcg ccgtggtgtc ggtcgtgcac gatctctcgc
     6481 tcgcgcggat gtacggaagc cgcggcgtgc tgctcgacca cggtaagatc gctgccgcgg
     6541 gagaggctgc ggatgtgttc gcgccggaaa cgctcaacgc cgtttaccgc atggatgttg
     6601 ccgactggat gcgcaccatg ctctcccaat gggaaaagtg aagaaagcag gaagcgccgc
     6661 cggaatcccg gcggcgcttc ctgcgtctgt tataaatgca taattatgcc gatgatttta
     6721 ttaatgtaaa gagataaaat gcggaaaatg gcgcgagggt attgacagaa ggagaacacc
     6781 gccgtataat ccgtgcataa aataagaaag cacctgatag aggtcgcgga aacgaagagt
     6841 atcttgcagc acacgccggc aggcggctgc aggagaaagg caattccgcc gaagcaccgc
     6901 atggctgccg ctgtgccgct gctgggccga cgcccaataa gcgccggact gtcacagtta
     6961 ctgtggagag ctatcaaagg aaaccggttt ttgattttct ttttgaatct catttttcgg
     7021 aaagcctgcg ataccacatc gggtcgcagg cttatttaat ttttccgaaa aggagacaga
     7081 aaaaatgaga agaagaaaca tccttgcgct gctgatggca gccgccctct gcctttgcct
     7141 gctcgccggg tgcggcagtg cagaaacgac cgacagcacc ggaacgagca tcacggaagc
     7201 cacgacgatc gccgatctca agggcatgaa gatcgcggcg caggccggaa cgttccacgc
     7261 cgacgcactc aaccagatcg aaaacgtgca gggatcgacg tatcccgagt ttgccgatct
     7321 tctgaccgca ctcaagagcg gcgcgatcga cggctacatc gccgaggaac cgacggcctt
     7381 ctccgtctgc cagagcgacg atactcttac gttcctcccg tttaagaaca atgagaccgg
     7441 ctttaccgcc acggcgtccg atgtcggcat cgccatcggt ctgaagaagg gctcggagct
     7501 gcgcgagcag atcaacgcca ttctcgcgga gatcacaccc gagcagcgca gcgcgctcat
     7561 ggagcagatc gtgacgctca gctccggcgg ggaagtgacc gagtttgccg tgagcgccga
     7621 agagccggaa aacccgacgg gcacgctccg cgtcggcatg gagtgcgcgt atgagccgta
     7681 taactggacc gacatctccg gcgcatcctt cggcgcggtg cccatctccg gcgagggcaa
     7741 ggacggcctt tacgccaacg gctacgacgt gcagatcgca aagtatgtcg cggcgcgtct
     7801 cggcatgaat ctggaaattt actccatcga atgggattcg ctgctgcccg cgctggaatc
     7861 cggcgcgatc gacgccattg ccgccggcat gagccccacg gccgagcgcg ccgcgcagat
     7921 cgacttcacc gatacctatt acgaatccaa cctcgtcgtc atcatccgca agtaatctga
     7981 ccgaaagggc cggtaaacag ccatgagctt ttttcaggac gtaatcaaca tagtcgccaa
     8041 atattatccc cagctgctca gcggcgtggg aaacacgatg ctcattgcgc ttaccggcac
     8101 cgtggccggt ctcgccatcg gccttctgac cggcatcgtc cgcacggcgc cattctcccg
     8161 caacggcttt gtgcgcgtgc tccataagat cctgaacgcc gtcatcgccg tttatgtgga
     8221 gatcttccgc ggcacgccca tgatggtgca ggcgatggtc atctactggg gttatgcctt
     8281 tgcctccggc ggcaacacgc tgccgctcat tccgtccggc atcctgatcg tttcggtcaa
     8341 caccggcgct tacatggccg agatcgtccg cggcggcatc atctccatcg ataagggcca
     8401 gtttgagggc gctatgagca tcggcatgac ccattcgcag acgatgttca aggtcatcat
     8461 cccgcaggtc atgcgcaaca tcctgccctc ggtaagcaac gagtttgtca tcaacatcaa
     8521 ggacacctcg gtgctgaacg tcatcggcgt aacggagctt tattactttg ccgggatcat
     8581 caagcgccag agctttgaaa cgttccagac gtatcttgtc gtgtgtgtgc tgtacttcat
     8641 tctgactttc acggtcacgc ggctgctgcg cttcgtggag cgtaaactcg acgggaacga
     8701 cagctatgtg atcttcggct cgcagagcga cagcgccgcc gagatccgca tcaaaaccgc
     8761 gagggagggc tgagccatgg gtgaaaaggt catagagctt gtacatctgc aaaagcagtt
     8821 cggcgatcat accgtgctgc gcgatatcca tctcagcgtg gaaaagggtg aagtgctcag
     8881 catcatcggc gcgtccggct cgggcaagag cacgatgctc cggtgcatca accttcttnn
     8941 nnnnnnnnaa cgccgacgtc cggccaggtg ctttatcacg ggcaggacat caccgcgcgg
     9001 gagatgaatc tctcgcagta ccgcgcaaag gtcggcatgg tgttccagag cttcaatctc
     9061 ttcaacaaca tgacggcgct gcaaaactgc gttaccggtc agatgtctgt gctccggcgc
     9121 agccgggccg aggcggaaaa aatcgccatg gaatatctcg ggcgcgtcgg catgacgccg
     9181 tacatccacg cccgcccggc gcagctctcc ggcggacaga agcagcgcgt ggcgatcgcc
     9241 cgcgcgcttg cgatggagcc ggaggtactg ctcttcgacg agccgacgag cgcgctcgat
     9301 cccgagatgg tcggcgaggt gctcgatgtt atgcgctctc tcgccggaga cggactcacg
     9361 atgatcgtcg tgacgcacga gatggcgttt gcgcgcgacg tttccacgaa cgtcgtcttt
     9421 atggccgacg gcgtcatctg cgaagaggga cgcccgaagg atatttttga aaacccgcag
     9481 gacccgaaga cgcgcgaatt tctctcgcgc ttcctcgtgg ggtgatacca aagaccgcag
     9541 cgctgccacg cgctgcggtc ttcgtatatc ggcccgaccg gttgacacgc gccggacggc
     9601 actggtatag tggagccgaa aaggaaaggg gggcgagata tggaacaccg cattttgctc
     9661 gtagaggatg accgcagcct cgccgacgcc atctgcgcgc agatgaagct ctgggcgctg
     9721 gagtgcacct gcgctgccga tttccgcgcg gtcatggcgg agtttgcgcg cgtgcagccg
     9781 catctcgtgc tgatggacgt gtcgcttccg tatatgagcg ggtatcactg gtgcgcggag
     9841 atacgaaaag taagctccgt gccggtcata ttcatctcct cggcgagcga taatatgaac
     9901 attatcatgg cgatgaacat gggcggcgac gattttgtcg ccaagccctt tgatacgaac
     9961 gtgctcattg ccaaggtgca ggcccttctc cgccggacgt atgagttcgg cgcggcggcg
    10021 cccgtgctcg agcacgccgg ggcgatcctc aataccggcg acaatacgct cacctatgcc
    10081 gacgagcaca tcgctctcac caaaaatgaa taccgcatcc ttctcacgct gatgcagaac
    10141 aaagggaaga tcgtcagccg cgaaaagctc atggaagcgc tctggcagac ggactccttc
    10201 gtggacgaga acacgctcac cgtgaacgtc ggccgcctgc gcaaaaagct cgaaaacgcg
    10261 gggctgcgcg atttcatcac aacgaagttc ggcgtgggct acgccgtggg ggaaaaatga
    10321 agctgctcgg agactatatc aaaagccgcc gcggtgtgct gctgttcttt gcgctgacgg
    10381 cggcgctgct cggcgtatcc ttcgccctct tccatctgcc gctgcgcgcc gtggcgtatc
    10441 cgtgcgcgct cgcgctgctg ctcggtctcg cggcgctcgc gctcgatttc cggcgcgtat
    10501 ataagctcca tcgggagctt tcccagctgg agaccacggc ggcggagctc atcggcgtgc
    10561 tgccggcggc ggcaagcgtg ccggaggcag actatcaggc ggtcgtgcag agtctctgcc
    10621 gccagagcgc ggacgccgcg gcaaaggccg ccgccgaccg cgccgacatg ctcgaatact
    10681 atacgctctg ggcgcaccag atcaaaacgc ccatcgccgc catgcgtctc cggctccaga
    10741 gcgaggacag cgactcctcc cgccgccttc tcgccgatct cggacgcatc gagcagtatg
    10801 tggaaatggt gctcacgtac ctccggctgg agggcgaggg gacggactat gtattccgcg
    10861 aagcgaagct cgatgatatc gtccgtccgg cgctccggcg tttcgccggg gagttcatcg
    10921 cgcgccgcct gacgctcgac tatacgccca cggacgtgcg cgtgttcaca gacgagaagt
    10981 ggctctcgtt cgtcattgag caggtgctct cgaacgcttt gaagtatacg ccgcagggcg
    11041 gcgtttccgt gtacgttgaa gagccgaaaa cgctctgcat ccgcgatacc ggcatcggca
    11101 ttgcgccgga ggatctgccg cgcgtctttg agcgcggcta caccggcttg cagggacgcg
    11161 cggacaagcg cgcgagcggc ataggactgt atctctgccg ccgcatctgc cggaatctcg
    11221 gccacacgat cacggcggag tcgcgccccg gcgaggggac gaccgtccgg atcaaattgg
    11281 aagaaaagaa gatcacagta gagtaaatgt gacagaaatg ttggatttgc cgtgcgagtg
    11341 taagccgatc cgatggaacg ggtcggcttt ttcgcttata nnnnnnnnnn tttccgggct
    11401 gaaaaaggta tataagaccc gcttcggcgg caatgcggtc gaggcgctcc gcaacgtcaa
    11461 tttctccgtg aacgagggcg agtatgtcgc catcatgggc gagagcggca gcggcaaaac
    11521 gacgctcctg aacatcctcg ccgcgctcga caagcccacc gccggcacgg tgctgctcga
    11581 cgggcgggac tttaccaaga tccgcgagag cgaggctgcc acgtttcgcc gcgaccatct
    11641 cgggttcgtg tttcaggagt tcaacctgct cgacacgttc acgcttgagg ataacatcta
    11701 tctcccgctc gtcctcgcgg gcaagagcta cgatgagatg cgcacccgcc tcatcgatat
    11761 tgcggaaacg ctcggcatcc gcgagctttt gaaaaagtac ccctacgagg tctccggcgg
    11821 ccagaagcag cgcgccgccg tcgcccgcgc gctcatcaca aagccgcgca tcctgcttgc
    11881 cgacgagccg accggcgcgc tcgacagcaa atccacggac gagctgctcc gtctcttcgg
    11941 cgaggtgaac cgcggcgggc agacgatcct catggtcacg cactccgtga aggccgcgag
    12001 ctgcgccggg cgcgtactct tcatccgcga cggcgaggtg ttccaccagc tctaccgcgg
    12061 cgacgccacg aacgagctct tctaccagcg catctccgaa acgctcacga tgctcgcggg
    12121 aggcggtgag gcgcgatgaa aaccgctctc tacccgcgtc tcgcgtggga cggcctgcgc
    12181 aagaacaagc ggctttttac cccgtatctg ctcacctgcg tgtgcatggt gatgatgttt
    12241 tatatcctct cgttcctcgg ctcgccggag acgtgcgcgc tgctgccgcg cggctccaac
    12301 accaccggaa agatcctcaa tctcggcagc tttgtgatct ttgtgttctc cgcgatcttt
    12361 ctctactata ccaactcgtt ccttgtccgc cgccgcgagc gggaattcgg cctctataac
    12421 gtccttggca tgaataagcg caatcttgcg cgcatcgtta cctgggagag cctcattacc
    12481 gccgcgctct cgctcgtgct cggtcttgcg ctcggcatcg tcctctcaaa gctcgcggag
    12541 cttggactgg tcaatatgct cggcggcgat atcaattacc gcatccgcat cgacgttgat
    12601 tccctgacgc gcgcgctggg actgtacgcg atcatcttcg cggtcatctg gctctccacg
    12661 gtcgtccgcg tcgggcgcag cagcgccgtg gcgctgctgc gcagcgagtc cgtcggcgaa
    12721 aagccgccga aggcaaactg gctgctcggc cttgcgggcg ttgtggtcct cgccgcggcg
    12781 tattaccttg ccgtgagcat caccaacccg cttgacgcga tcgtatggtt cttcgtggct
    12841 gtgatcctcg tcatcatcgg cacatatctt ctgctcgtcg caggcagcgt acttctctgc
    12901 cgcgtgctgc agaagaataa gaaatactac taccggccgg agcatttcgt gtccgtttcc
    12961 tccatggcgt accgcatgaa gcgcaacggc gcgggccttg cgagcgtgtg catcatcgcc
    13021 acaatgatcc tcgtgatgat ctcctcttcc tcgtgccttt atttcggcgc ggaggactcc
    13081 gcgaatatgc gctacccgag agatataaac atcactctcg ccggggagac gccggagcag
    13141 atcacggacg gcaatatcga gcgctaccgc cgtgccatcg ccgattatac gtcagagcgg
    13201 ggcgttccga cgaaggacgt gcagaactac cgctatatca ataccagcgg tcttttctcc
    13261 ggcgacacgg tgcagtgctg gtattcgcgg gagggcgtct cggcctcata ttcatattcg
    13321 gagattcgtg acatctatat cgtttctact gcggactatg ctgcccgaac cggagagaat
    13381 gtttcgcttg cgccggacga ggtgctgctg ctgacctaca aatgctccta tgaccggccg
    13441 acgctgacgc ttgctatgga cgacgtctcc cacacgtggc gcgttgccga tgtgcgaaac
    13501 ggttattttc tcccgccggc ggtcggtgga tttatcgcgc aaatgacgct catcgtgaac
    13561 gacacggcct ccgccgttca ggatttccgc acatataacg ggcatgtggt tacgatgcag
    13621 tataaatggg tctacaattt caataccgac ctcggcgatg aggagtgcgt ggcgctcaca
    13681 cagggcgtca tgcgcaccct cggcgaggtg gagccggagc agggttatat gtacctcctg
    13741 gctgagtgcc gtgcggacgg aagcgccgat ttctacggca gcaacggcgc gctcttcttc
    13801 atcggcatca tgctcacact ggtattcatg ctcgcggcgg tgctgatcct ctactacaag
    13861 cagatatccg agggctacga ggaccagaaa cgctttgaga tcatgcagaa ggtcggcatg
    13921 acgaaaaagg agatccgccg cagcatcaat tcccagctgc tgaccgtatt tttcctgccg
    13981 ctgctgctgg cgggcgtaca ccttgcgttc gccttcccga tgatccggcg gctgctcgag
    14041 ctgttctcgc tttacaacgt cggcctcttt gtcaccacga cgctcgtgag cttcggcgct
    14101 tttgcgctgc tctatacgat cgtctaccgt ctgacggcga aggcgtacta caacatcgtg
    14161 agcgcatgat tttttccaaa gaaggcgggg gcgactccgc cttctccttt ttaaacaaat
    14221 cttaaagacc ctttaatgga ttttgctctg cggtgcgtga tatccttggc atcacaggga
    14281 aaggagaaag cgccatgcaa accgtgaaaa tgataaactt catcctgatg tgcctgtttt
    14341 ttgcctgcta tatgtatcag tttctgtaca tacccgtttc gtggctgccg cggaagcggc
    14401 gggcgcggga agcgccgctg caccgcttcg cggtcctcat cgccgcgtgc aacgaggaga
    14461 ccgtcatcgg caatcttctt gacagcatca aggcgcagag ctaccccgaa cgctttctcg
    14521 aggttttcgt cgtggccgac aactgccgcg ataaaacggc ggaaacggcg cgcgcccacg
    14581 gcgcggaggt ctatgagcgg cacaacgctt cgctccgcgg caagggctat gcgctcaact
    14641 tcctcctgcg cgaaatggag cgccatgcgc acaacgagtt tgacggctac atcgtcctcg
    14701 acgccgacaa cgtgctcgag cgcgatttcg tctcccgcat gaacgagacg ttttccgagg
    14761 gcgaggacat cgtcacctgc taccgtgact cgaaaaacta cggcgacaac tggatatcgg
    14821 cgggctacgg cctgtggttt ctccgcgaat cgcgctatct gaacggcgca agatcgcgca
    14881 tcggggcgag ctgcggcgtt tccggcacgg gctttctctt ttcacgcgcc gtgctcgaag
    14941 ggcagggcgg cggctggccg ttccacctgc tgaccgagga tatcgagttt accatcgaaa
    15001 acgtgacggc gggacggcgc gtcggctact gcccggaggc cgtgctctac gatgagcagc
    15061 ccgtccgttt caagcagtcc tgggcgcagc gtctgcgctg gtcgcgcggg tatctgcagg
    15121 tgttccgcaa atacggcgga aagctcctct ccggcatgtt ccgcggcagc ttctcctgct
    15181 acgatatggc gatgagcatc atgcccgcgg cggtgctcac ggggctgagc atcgtggtaa
    15241 atctcggcgc ggcggcgatc aacgtgttcc attaccatga gtgggacgtg cttgccatat
    15301 cggcactgca gacggtgatg aatctttatc tcacgctctt cgtcctcggc gcggtcacga
    15361 ccgcgacgga gtggcgcagc atccactgcg cggcgtggaa gaagatcgcc tatgtgttca
    15421 cgttcccggt gttcatgctc acgtatgtgc cgatcgtggt gcagtcgctc ttcgtaacgc
    15481 ccgagtggac gcacatcgac catacccggg ccgtcacggt gcagcagatc tgcgcggcgg
    15541 attgccgcga cgccgtgtaa aatcgcttta acgcttttta tccgatatac gaaaacagca
    15601 tcgtccgaaa gaatatcgga cgatgctgtt tcctgtcagg agatgaaagc agaaattctc
    15661 ttgcttatac tcagcggctt tgccatggct ctcgcggaca gtgtctccgg tttgcccggc
    15721 ggaacggccg cgttctgctc ggcttccgtg accggttcgc cgccttgctc gacgcgctcg
    15781 tccacggcgc gcgggacgca aaaaaaagcg cgaattattt tccggcggtg tggacaaaaa
    15841 agagcatttg cgctaaaata cgctccgcag gaggatgaac tatgaaaacg aaaaaaaggc
    15901 tccgcgctct gacgggagcc ataacggata ttgtcctggc cgcggtgatt ctgcttgtat
    15961 ttgcttattt ccaccacggg aggatgtatc ttctgggacg gggcttcgtg gaagcgcccg
    16021 ccgcgacggc gcagcccacg gccgccgcag cggcgcagcc gacgcccgaa acggaagcgc
    16081 cggacgaaac gctggtcgcc aacggcccgt ttaccgacgg gacgatagaa cagacggaaa
    16141 actcctaccg cagcgccaat gtcagcgtga gctggacgcg gcacgaggac cgctcgaacg
    16201 acgggctcgt cgtatactat gtcgcggata tctggctgcg ctcgagcgag tatctgcgca
    16261 cggcgtatgt gacgaacggc taccggagcg tacacgatat ggccgcagaa tccaacgcca
    16321 ttctcgccat cgacggagac tatgccttct cgcgggaata cgggcccatc gtgcgcaacg
    16381 gagcgttcct ccgcgacacg ggatttgacg acatacttgt atactatacg gacggctcca
    16441 tgcggatttt ccgcggcaat gcctttgaca tggatgcgga gatggaaaag ggcgcggtgc
    16501 aggtctggtc gttcggcccc gccctgctgg acgccgaggg gaagagcatc gacaaattca
    16561 acacgaacat cgccggactg aatccgcgcg cggcgctcgg gtattatgag ccgggacatt
    16621 actgttttgt ggtcgtggac ggccgcgggg agaacggctc gcgcgggtat aagctcgagg
    16681 agctctcccg cctctttgaa agtctcggct gcacgctcgc ctacaacttc gacggcggca
    16741 aatccgccgt tatggtctgg gacggcgata cgaccgtgaa cacgccggcc ggcggcggcc
    16801 ggaacgtgac ggatatcctg tacattgcaa aggagtgacc gccggtgaaa ttccgaaatc
    16861 tgctctcgca cctgacggcg gcgcttgcgc tcgccgtgat cgtgatcctc gtgatcgatc
    16921 tctttttcaa cagcgccatg tatctcgctg ccggacgcga gttcaagtat ctctgcatgg
    16981 cgctcagcgt gtgtgcgctc gtgtgcgccg tcatgcaaag gagagaaaca aaatgaagct
    17041 ctgtttcaag cagcggtttt tctcgtggtt tgacagctac gacatttacg acgcctctac
    17101 cggcgcaacg gctttcactg tggaaggaaa gctcgcctgg ggacactgcc tgcacattct
    17161 cgacgcgcag gggaggcaca tcgccacagt aaaagagcgc gtgctgacgt tcctgccgaa
    17221 gtttgatctc tacatcggcg agcggagcat cggcacgatc cgcaaggagt tcacgctctt
    17281 tcgccccgcg ttcgtgctgg acttcaacgg ctggcgcgtg cagggcagct ttctggagtg
    17341 ggactatacg atcgaagacg cggcgggccg caccgtcgcg cgcatagcca aggagctcct
    17401 ccgctggacg gatacttaca ccatcgacgt accggacgcg agagacgcgc tgtgcgcact
    17461 catggtggtc ctcgccattg acgccgaaaa atgctcgcgc ggcagcaatt gaagctatat
    17521 taacgtcata cgcccgcgga gaaactctcc gcgggcgtat tttgtcgtat gcggcggtca
    17581 ttccgccaga acctttttca ccatgacgat ttggtccgtc cggtatttga ccgagaaacc
    17641 gcagcggagg aaaagtgcga cggacggatt gtccgcggca atgtcatcgt ataaaacgcc
    17701 gacgccgttt tcctttgccg ccgcgcacaa aagcgcaagc cccgccgcgc cgtagccccg
    17761 gcggtggtaa ggggcgtaca cgatcacgtc ggcaagatat ttttccccgt caaagtgata
    17821 ggcgatctcc ccgacgaact cgccggaagc gctctcgcgg agatagcggt aataccgtcc
    17881 ctccgggtgc gcgacccagc ggccgtacca gtc
//
LOCUS       DAWAAE010000025        16979 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1530_length_16979_cov_3.254195, whole genome shotgun sequence.
ACCESSION   DAWAAE010000025 DAWAAE010000000
VERSION     DAWAAE010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 16979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16979
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_16979_cov_3.254195"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9670..9679
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12479..12488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14788..14797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3207 a         4953 c         5333 g         3456 t
ORIGIN      
        1 cgctgaggcg ttcgcgccga gcgcgccgac catcgcggag tcgatgtact gcatggcgat
       61 cgtcgttatt tgtgtaagta tcgccggaag gctcaaccgc cagacctcgc gggcctgcgc
      121 cttcagctcc ggcatcgttt ctcttttcat atcatcatcc ggtccgcgcc gtttttattg
      181 acggggcggt tttttctggt aaaatataag aatacaaatc tttcaagctt tcgggagcat
      241 gcccaatgaa gaaactgaaa aaacgcttca aaaacatcaa gccgcagacg ctgctcacgc
      301 acgttatcgt aacgcttctg tatccgcttg cgcgtgtggt catggcctcg tcgcagcggc
      361 tgctcgtctt caccgactgc ctgacgatcg tcgcggcgat cctgctcatc gcgggtgttg
      421 tctactcgct ctatctccac ggcgactttg acatcagcag ctatgtgatg cgccgcgcgc
      481 gcggcaatgc gccgaagcag acctttgagg cgtacaaggc gaaccagaaa gaaagaagag
      541 aggcgtcttt caactatcct ctcttccttg gagccgtata tatggcggcg gcgcttttta
      601 tcgcctacgt cttttactga ttctttgcgt tctcgatctc ggcgagcttc ttctcgtagc
      661 cttccatctc tttgtccgtg gcaaagacga agtgcccggg gcggatctcg cgcagggagc
      721 gcagcgcgcc ggactggtcc agcttggagg ggtcgtacac gatggcgcgg cgagtccgtt
      781 caacgaccgg gtcgggcatc ggcacggcgg agagcaggga gatggtgtac ggatgcagcg
      841 ggcactggaa cagtgtctcg gaatccgcga tctccacgat ctcgccgaga tggatgacgg
      901 cgatgcggtc ggcaatgaac cggacgaccg aaaggtcgtg cgcgatgaaa aggtacgtga
      961 gattcttttc cgccttgagc ttgttcatca ggttgagcac ctgcgcgcgg atggaaacgt
     1021 ccagcgcgga gatcggctcg tccgccacga tcagcttcgg gtccatgatc atcgcgcggg
     1081 caatgccgac gtgctgacgc tgtccgccgg agaactcgtg cggatagcgg gacttgtgct
     1141 cgggcagcag gccgacgtcc ttgagcgcct gatcgacctt ggcgatacgc tcttcctcgt
     1201 ccttataaag gtggaagttg tacagaccct cggagatgca gtactcgatg gtggcgcgct
     1261 cgttgagcga cgccgccggg tcctggaaga tcatctggat gttgcggacg acgttgcggt
     1321 cttcctcgcg ggagatcttg ccggaaatgc gcttgccctc aaagaggatc tcaccgttgc
     1381 tgcacgggtt gatgcgcatg atggcgcggc ccagcgtcgt tttgccggag ccggactcgc
     1441 cgacgagggc aaaggtctcg cccttataaa tgtcgaaatt cgcgtctttt acggcgacga
     1501 attttttttt gcctcctacg tcaaaggaga tgtccacatg gctgatcgag aggatgacct
     1561 ctctatcggc gttattattc gacatggaag gcacctccct tatcggtcat cttcttgatc
     1621 ttctcgtgca gatcctgaat gatctccggc ttttcgagct tcggcgcgcg gggatccaga
     1681 agccacgttt tggcatagtg cgtttcgctc accgggaaga acggcggctc catttcaaag
     1741 tcgatcttca gcgcgtgctc attgcgcggg gcaaaggcgt cgcccttgat ctggttgtac
     1801 agagacggcg gagtaccggt gatgtagtag agctcctgcc ccttgatgcc gagctgcggc
     1861 agactggaga gcagcgccca ggtgtacggg tggcgcggat cgaagaacac ttcctccacg
     1921 ttgccgtact cgatgatctg gccgccgtac ataacgccga cgcggtcggc gacgttcgcg
     1981 acaacgccga ggtcatgcgt gatgtagatg gtggtgaact tgagctcctt ctgcagatcg
     2041 cggatgagcg caatgatctg cgcctggatc gtaacgtcca gagcggtcgt cggctcgtcg
     2101 cagatgagga tcctcgggtg gcaggagagc gcgatcgcaa tgacgatgcg ctggcgcatg
     2161 ccgccggaat actggaacgg atactcgtcg aagcggttgg cggcgttctg gatgccgacg
     2221 cgctccataa gctcgatggc ctgcttcttc gcgtcgccgc gggaaatgcc ctggtgcttt
     2281 tcaatgacct cggtgatctg cgagccgatc gtgcggatcg ggttcagcga cgtcatcggg
     2341 tcctggaaga tcgtggagat cttcgcgccg cggatgcctt cccagtcctt atcggtcttg
     2401 agcttggtga ggtccttgcc ctcgaacatg accgagccgt tggtgatgga gccgttggac
     2461 tcgagcatgc cggtgaacgt cttggtgaaa acgctcttgc cggagccgga ctcgccgacg
     2521 atggcgaacg tttcaccctc gtaaaggtcg agagacacgc cgcggatggc cgtgaggtaa
     2581 ttgtcacgga cgcggaattt aacttcaatg tcctttgcgg acagaacgat gtttctgtta
     2641 tctgccatta ctctgccccc ctcacatgtg cgtgcgcgga tcggcagcgt cggcgagctt
     2701 ctgtcccacg atgtagaggg agatggaaat aaacgccaga acgccgaccg ggatccagaa
     2761 caggtgcgga tagccgttca tataagaggt atagagcgag atggactggc cgagggacgc
     2821 gttggtcgtg ccgagaccga gacccatgta ggagaggaac acctcgtagg agatgagcga
     2881 ggggatctgc cgggacacat acgtcatgat gacggagatg aggtacggca gaatgtttct
     2941 caagatcatc gtccacgtcc tggtgccgag gcacttggac gccatgttgt actcacggtc
     3001 gcggatgatc atgacctgcg tgcggatgaa gtacgcgaca aagatccact cggtacacgt
     3061 catcgtgatg atgagcgagc gcataccggc gccgaggatg taggagagga tcatggcgag
     3121 cagcaggaac gggacgttgg aaatgatgtt gtagacctcg atcatccact tgtccatctt
     3181 cttggagaag ccccagagcg cgccgatcgg aatgccgacg agcatattga tggccgtgca
     3241 gacaaaggag atgatgatgg agtttctcgc gcccgcccag acagcgtcaa atacctcgtt
     3301 gccgacgtgg tcggtgccga agatgtgctc ggcgctcggc ttcaggaact tcatctcggc
     3361 gttgttgatg ttcggcgtgt cgatggggct gtagccggag cgcgtcggct gaatgagcgc
     3421 gaacgcgaca acggcgatca gcaggatcga tacgacgacc gtaaacttgt tcttgaagaa
     3481 cgtgcggaac acggacttcc agtaggagta gcgcggagcg gcaatgtgct ccgattccag
     3541 ctcgtcgaag gctgcaaact caaagaggct cttatcgata ttttcggtat tcatttaaga
     3601 cctccctgcc ttctcgctga gcgaaatacg cggatcgacc ttcgtgagaa caatgtcgcc
     3661 cgccagcacc gacagcaccg ataccgtgga gaagatgaac gcgagcgcca cgatcataac
     3721 gttgttgtac tggttgatgg cgttgggcag cattttgccc atgccgccga cggagtagat
     3781 cgactcggtg atgatcgcgc cggtgatgca ggccgcgaga gacgcgggga tgccctgcgc
     3841 gatggggatg acggcattct tgaaaatatg tccgctgaag atctcgcgct ggttgaggcc
     3901 cttggccttg gcaaacttca catagtcgga gttcatctgg tccacaacat agcggcgcgt
     3961 ccagagcatg aggctcgcaa tagacggcag cgccagcgag atgatgggca agatccagga
     4021 tcgcacgtca cccgggccgt aggtcgtaaa gaccgacggc agattgaaga gcgttgtgcc
     4081 gagatagcgg aagagataga tgtacgcgag cgacggcacg gcgatgatga agatgatata
     4141 caccatgccg agcttatcgg cgaacttatc cttgtttcgt gccatgagaa cgccgaacgg
     4201 cagtccgaga acgtaggcga ccgccatggc gaagagaccc atgatgaacg aggtgccgat
     4261 catcgagggc tggtttttct gggtttcaaa ggacgcgtaa tggtcggtga attttttcat
     4321 gtccattctg tcgagaacgg acttatactt aagcgagccg aaaatgattc cggactcggc
     4381 ttcataaccg gtttcaaagg tgacagggcg cttgacctcg gtgccctgcg actggaaaag
     4441 cacgcggagt acgtccaggc cctgataggt cggataagag gtaccgaagt tcagcgtgat
     4501 aatgttctga tgaatccacg ggaagcggct gtcggtatac agcaggtact tgtgcgtcgt
     4561 gccgctgccg atgagagcca cgccgccggt gggcgtcgtg ccgaagcgaa ggccgcgctc
     4621 aagatccggg ttcgtctcat ccttgacaga ccagggagta tcgatcacga tcagatgggc
     4681 aagccagttc gccatgcgct tgatgacggg cgtgtcctta taggcatagt aacgtccgct
     4741 ctgcagatag ttggcgatcg tatagccctg cgcggaatac tgctccacaa aggcctggct
     4801 ttccggagaa tccggctcga tggcggcaat catttccggg ctgccggctt catagagatt
     4861 aaggcagtag tcgttgatgc ggacgtagtt cagatagccg agcttctgcc aggttgaata
     4921 aacgtattcg gttttatcgt cggattttcc gccgagcttg cggtatgtgc tgtcttcaaa
     4981 gaagatgttg tcacgcggca ccaacgtata gaccatcgta aacacgatga gcatgatgat
     5041 caaaatggag acgagcgagc gcagcaggcg gttccagata tatccttttc tcataggtgg
     5101 taccgttcct tacaaagata gcatcggttg acaggggaca aaaggcccct gtcaatcggt
     5161 acaagaaaga gccgagaagc atggccgccc ggctctctct gttcaattag tcagtgcttg
     5221 tgctgttgct gttcagcaat tattcgccgg ccatccaggc ttccttcgcg gcttcgtact
     5281 cggcagtagt cacaggctcg tcgaggagct gcatgtactt gaagtacggc acctggttgt
     5341 aagcagcctg gccgtagaga ccgtagccaa cggtgaaggg cttcaccttg ctgatggaga
     5401 tgttcgcgcc gctggtggag taaggacgga ggatgccgtt ggtgagcagc atggcctcgg
     5461 ccttcgcgaa gagctcgatg cgctcgtcgc cggtggcggc ggtagcctga tcaatgactt
     5521 cctggacctc attgaggccg agggtctcga gaacggcctt ctgctcttcg gactggccct
     5581 ggctctcgat gttgatgccg gagtagttga gcatgtcgcc gtcgtggacg tcgaagcagt
     5641 gcaggtaggt cagaggatcg ccgtagtcgg gaccccagcc ggcgccgaac acgaggtcga
     5701 tggagttctc ttcaccggtc ggcatgttgt agaacgagga gtagtagacg gaggagttgc
     5761 cctcgaggaa ctgcaggtcg atgacggcgt aatcgccgat ggcgtcctca atggacttct
     5821 tcatcgcgag ctgcatgttc ttctggttct cagaagcggc gtagacagga acgtcgatgt
     5881 ggatgggcca ctcggagacg ctgtcgccca gctcttcctt ggcgcgctcg gcaaaggtct
     5941 tggcgagctc ggggttgtac caggcgtcgt gaccgtcgtt cagatcgatg ccttcataat
     6001 cggcaacgag ctcggcggcg tacttctcaa cgatggagcc gtagctgcgg ccgtcggtgg
     6061 tcatggcgaa ggtgtaagga acgagggtgt tgcggatgac gccgtcggcg tgctcctcgc
     6121 cgagagtgat ggcctcaacg gcgtgcgcgg agtaggcggc gtaaacggcg cggcggaagt
     6181 cggcgttcag aatggcggcc ttggcgtcag ccttctgggc gtcggtcttg ttggtggtgg
     6241 aaacagaggg atcgttgtac agagcgtaca gctggcgatc ccagttgaag gcgccccagt
     6301 aagaggtggc ggtggtcatg ctcttgtact ggttgtcggg atagagctcg gcagccttcg
     6361 caacgacctc gggcagggac tggttgacgg tggtggaggt gacttcgccg ttgacgaaca
     6421 tgttgaagtt ctgggcggga tccttaccgt cggagtaagt cacgacaacg tgctgaacga
     6481 aaacgttctt cgcgtcgtag tagttggggt tggcgtcgaa gcgcacttcc tgcgccggga
     6541 cgagagagga caggatgtag cagccgttgt agaggatgga gtcgggttca acggcgccga
     6601 agtcggagcc cttggactcg aggaactcgg cgttgatggg ccacaggatg gtgtaggtgg
     6661 tcatgccgtc gaagtagccg cagggaccgg tcagggtgta ggtgagctgg ttgccgtccg
     6721 ccttgatgcc gacgtcttcc cacttgccgg tgccattgga gtactcgcgc aggccggcga
     6781 tgaggtcagc gacgaggccg agggtctcgc tcttggagtc aacagcgtgc ttcagaccgg
     6841 tgacgaagtc ctcagccgta acggcgtcgt actcttcgcc ggcgctggtg gaccaaacgg
     6901 cgtcgtcacg aatggtgaag taccactcgg aagcatcgtc gttgcagctc cactcggaag
     6961 ccatgccggg gacgagcttg ccgtggttgt cctgggtgag caggccttcc acgaagttgt
     7021 tgacgtagtc gccgttggag gacttgttgt tgtaaacata gtccagcgtg gtgatgtcga
     7081 agttcgcgat catggagaaa gtgtccggac cggtgtactc ttcgacaggc tccgcggtcg
     7141 gctcttcagc cggctgctcg gccggggtct cagcaggctg ttccgccggg gcttccgtgg
     7201 gagccggagc aggagcctcg cttccgcacg cggccaggct cagcaccatg acaacggcaa
     7261 gaagcagcgc aaggaatttc ttcattgttt gttctcctcc ttattggttt ttcaaacttt
     7321 tttattaaac gcgggaagcg ttaataaact ttatgtattt ccggcgcttc agtcctcaaa
     7381 gagccgcgcc atggaaatgc cgagcttttc gctcatgaga aagagcgttt cgagcgacac
     7441 gcccttgacc tcggcggcat tgttcgcctc gatctggctc acaaagctca cgctccggcc
     7501 gatcgcctcg gcgaactgct cctgcgtcat gccgcgcatc ttgcggtagt aggcgatccg
     7561 cagaccgagt attctgaatc gttctctgta gtcctcacgc atacattttt cccctcacgg
     7621 taaacccgtt taccggagcg tggttagtat gtacaaagcc ccccggttcg atttactaat
     7681 tttaacgagt aaagcataaa atagtaaatc atccaatagt gtgtttttct acactcagag
     7741 tgtaacattt ccgtacagcg gcgaaaattg tttcactggg acggacgaaa tccgtcattc
     7801 tgccgaaaaa cggacgtttt tcctccggcg cggcggtcac tccgcgccgc tgatctcgac
     7861 gtattcgcgc aggccgtcgc gcatggtctc ctcataaatt tcccgcgcgc cggatacgga
     7921 gtcgagcgcg ctctcgtcgc aggcgagcgc gtaggcgacc gagccgtccg agcaggtgaa
     7981 ggcgtaaaac ggcaggccgc gcacgctgcg aagctccacg gcggtcacgg agaccgtacc
     8041 gtcgtcggcg ctgtcccatg tgacggcatt cgacgccgct tttttgatgg caagcgccgg
     8101 aacgtccgcg gcgccgtcga gatacaccgc gcggaggacc gcctggttga agtcgccggt
     8161 ataaagaaaa ctgaagcgca cgctgcactc gctctgcgcg gtgccgcccg tgccgcggaa
     8221 ggtgatcacg ccgttgtcgt tctccgccgc cgtaccgagc tccagcccgt aattgcgcag
     8281 caggccgatg agaccgctgt gctccagaaa cgtccgcgcc gcgttttccg gctgggaaga
     8341 ggcaaggaac gcctcctgct tatcgtaatt gtccggcgtg agcgccgggg cgttggggtt
     8401 ctgcaaaagg agcgagccgt tttccgaaac ggaatatgtc acgctgcccc agccgtttgc
     8461 ggcgacgttc gccgccacgg tctcgccgag cgccgtgaga tccacagact tcaaaacgcc
     8521 gagcgtcacg gtatcgggcg gggtgtaatc gcagtcgaac cggttgacgg aaacgcgccg
     8581 cccgctgccg gtcttgccgg tttcgatggg cgcggcggtc tgctccgtct gaaaggcgct
     8641 gccgcccgcg ccggggtcaa gctgcggcac ggaaaggggc cggtcctccg tgtcgggggt
     8701 cttgtcccgt ccgggaagca gcagaatgat ggcggcgacc gcggcgataa gcatcacgcc
     8761 gaggaatatc cagcccgcaa gcatgacctt cttctgcgaa gcccgccgcg cgcggcggga
     8821 ggtattttct tcctgacggg gttttcggta gtttttcatg gtgagttcct tttgaagcct
     8881 cccttgtgta aagggaggtg ggttttgccg caggcaaaag ccggagggat tgtcgtttac
     8941 ccctcagtca gccttacggc tgccagctcc cctgttaggg gcgccaagtt ggttttctgc
     9001 aagcccactt gcctcccctc gacaggggag gtggcaaaaa cgaagttttt gacggagggg
     9061 ttatcatttt ccgaaaacta caatccctca gtctcgcttc gctcgacagc tccctttaca
     9121 caagggagcc tttttcgttt atgcgagcgc tgcccaggcg cagccgagga gcgtggcatc
     9181 gccggcgaag aggaattccg cgccgccgag accgtgccgt ttcgtatact ccgcgatcag
     9241 cgcttccatc cggggacgca gcacggggtt gagcttgagc atcgtcccgt ccgcgcagat
     9301 gccggtgagc atccccgcgc cgaagccgcg ctcgcgcaga atggcgcaca ggcacgccgc
     9361 ggcgatgcgc gccgcgcgct cgagaagcat cgtgttgatc tcggaagcga agcggcggtc
     9421 cttctccgtt tcgagcgctt cggcaacggc gttgccgccc atggggtcaa gacaaaaagc
     9481 gtccaccatt gcgctcgtaa cggagagcgc ggcgatattc tccccgctct tcgccgagag
     9541 aaatccttcc tttgccgcaa ggagcagcgt ttcgcgcagc agcagacggt agtatgcccc
     9601 ggagatcatt ttttccgccg ggtggtcgcc gggcagcccg gaggcctcgt ccacggcgat
     9661 gtccgccgtn nnnnnnnnnc gcgcggccga agcagccgga ttcgagattg acgagcgttt
     9721 cgccgcccat cgccaccgtt tcgggcgatt ttgtgacgtc gcacgccttc acatggcagc
     9781 aggcgttcgt gccggtgccg agaataaagc cgatgaagtc ggcgtagcgc gagcggtcgc
     9841 acgatgccat gccgccgagc atggagccga cggtgtcgtt gatgagccgc cagcggcgag
     9901 cgccggacgc gccgagcgcg cgcatcgccg tttcgatgcc ctcgcatacg agctttccct
     9961 ccgcgccggt gacgcgcgat tccttgccca tgctgatgat gcgcccgtcg ccattcggca
    10021 ggcactcgca cgggaacgag aacgcgatgc acgcgcggtc ggcgtcgagc gcaacggtga
    10081 gcgcaagctc cgcaaaggcg cggaacatct cttccgcgct cgtgtccttg ccgagcgtgc
    10141 cgggcatacg gcgcttttgg agcgcttccg tgacgggctt tcccgcctcg tcaaagcgga
    10201 cgcgtccggc gcgcagattc gtgccgccgg cgtcgagcac gagaacgctc tcgcccgtgc
    10261 gcggcgcgct cgccgcggga aggaacgacg gcagcatcat cagcgtagag gcctctccgc
    10321 gcagcccggc gcgcatgtcg gcgatgtagg cgtccgtcag atcctcaaag ggctgctgct
    10381 catagaacat gttgtgtcgc cggagaaatt ctttggcggt ttcctttgta cacatagcgg
    10441 tctctccccc gttcgggcgc gttttccggc gtccgggatg taatatttct tcggtcaggc
    10501 gcgctcgagc ggcgtgcatg tattgccgga gatcttccag cggctgcagc cgccgttgaa
    10561 cgcgaagggc gcgaagtaga tgtccgtcgg cccgcagtgg acggagccgt ccgcagcggt
    10621 ggaccagcct tcgccggtgt ggggctttcc gtccgggtcg gtgatggtga aaaggccgtc
    10681 gttgcgcagc tccagcttcc attcgccgcc gtccgcgtcc acataggtgt acgttcccgc
    10741 cgggacgggc ggggcgggcg tgggcgtggg ctccgccggg gtgggttccg ctgtcggctc
    10801 cggcgtttcc gtcggggcgg gcgtcggcgt cgcggtcggc gtgggcgtgg gcgcttccgt
    10861 cggttccggc gtaggctccg ccggcggcac ggcggtgggc tcgctcgtgg gaaggggcgt
    10921 ggtctccggc gcggcactcg tgccgcacgc ggaaaggatg ccgagcgccg cgatcagtat
    10981 ggcggcggcc aggcgtttcg tgcttcttct catgggctgc tctccttagt acaaattttc
    11041 acacatatac gatacaacat tttttgcttt ctgtaaagcg tcgatcagca agggcacacg
    11101 catttccgcc cggcagaagc gcgcgccatt gacgatcggc gcagcctccg ggacgtggta
    11161 aaatctttca caggctcttt tcagcggctg acgagtatgc tatactttgg ttataagaca
    11221 agtataaaaa aaggggggct ttctccttgg atacataccg cccggaacag gcttggatcg
    11281 acgggacgaa tttcgacgcg cagaagctct tcggcgcgcg cgcagagccc aacggcgtat
    11341 cgttctccgt atgggcgccg aacgcgcgga gcgttcgtgt gacgggcgat ttctgctcgt
    11401 ggagcgacag cgcgcactgg ctcagccgcg gcgatctcgg cgtatggcgc ggctttgtgc
    11461 cgggcgcgcg cgccggacag ctctataagt acattatcga gggcgcggac ggctccgttg
    11521 tatggaaggc cgatcccttc gcgctcgcgg ccgaggtgcg ccccggcacc gcctcgcgca
    11581 tccacactct ctcctataac tggacggacg gcgaatggat ggccggccgc cgcgcgctcg
    11641 gcgggccgcg gaatatttat gaagtccacg ccggttcgtg gcggcggcat ccggacggga
    11701 gcttttatac ctggtatgag cttgccgatt cgctcgtgcc gtacatcaag gacatgggct
    11761 acacgcatct cgagcttctg cccgtgacgg agcacccgtt cgacggcagc tggggctatc
    11821 aggcgacggg ctttttcgct gccacgtccc gcttcggcga gccggagggt ctgatgcatc
    11881 tcatcgacga gtgccaccgc gccgggatcg gcgtcattct cgactgggtg ccggggcatt
    11941 tctgccgcga cacgcacggt ctcggccggt ttgacggcac ggcgctctat gaatccggcg
    12001 acatgcccgg ctggggcacc tataaattca actacgcgcg caatgaagtg cgctcgttcc
    12061 tcataagctc ggcgcttttc tggctggaag agtaccacgc cgacgcgctg cgcgtggacg
    12121 gcgtgagcag catgctctac ctgaattttg gcgtggacga tcccgcgcgg aaaaagtata
    12181 acgagcgcgg cggcgaggag gatctcgcgg cggtggcgtt tttgcagacg ctcaacaccg
    12241 tcatcggcaa gcgcgtcccc ggcgcggcga tgtatgccga ggagagctcg gcatggccgc
    12301 tcgtgacgta tccgccggag aagggcgggc tcggcttcca ttataaatgg gatatgggct
    12361 ggatgaacga cacgctccgg tatatgtcca tcgacttcga cggccgcaaa tataaccacg
    12421 gccttctgac gttctcgatg atgtacgcct tcagcgagaa ctacattctc ccactcagnn
    12481 nnnnnnnnca cgacgaggtc gtgcacggca agcgctcgct catcgggcgg atgccgggcg
    12541 actggtggcg gcagtttgcg ggcgcgcggc tgctgccgct gtatgcggcg tgccaccccg
    12601 gcggaatgct cagcttcatg ggcaacgagt tcgcgcagtt catcgagtgg cgcgattacg
    12661 aagagcttga atggttcctt ttgcagaacg cgacgcacgc gggcgtgcag gcgttcatcc
    12721 gcgagctgaa ccatgtttac cgcgcggaaa aagcgctctg ggagatcgac aacagctggg
    12781 acggctttga ctggatcgac gcggacaaca acgagcaggg catcctctcg ttcgtccgca
    12841 aagcgaagga cggttccgct attctctgcg tgctgaactt ccgtcccgaa tcgcatgaga
    12901 gcttccgtct cggcgtgccg ctcgcgggcg cgtggcagga aattttgtcc tcagacaata
    12961 cgcgcttcgg cggcagcgga aagacaaacc cgcgccgtct gcatacaaag aaaatcccct
    13021 cgcacgggcg cgatgcgtcg ctcgtgctca ccgtgccgcc gctcggcgga acgctcatca
    13081 aatacatagg agaaacgacc catgcccgga agagaagagt ttaagagaga atatctggag
    13141 agcatcgccg agacctgctc aaagccgttt gccgagtgct cgcgcatgga gcgctatacc
    13201 gcgctcgcgg cgatgatccg cgaatgctgc tcgggcgtgc gcgccgaaac gctgcgccgc
    13261 cgcgccgccg ccggagaaaa gatcgtctat tacttctcga tggaatttct cgtcgggcgg
    13321 ctgcttgaaa actatctcat ccagctcggc atccgtgaca tgaccgccga cattctcgcc
    13381 gagctcggcg aggatctgga cgcgctgctc gaatgcgagc gcgaccccgg tctcggcaac
    13441 ggcggtctgg gccgtctcgc ggcgtgctat ctcgattcgc tcgctgcgga ggacgtgccg
    13501 tgcgtcggca tgggccttcg ctaccgttac ggtcttttcc gccagcgcat ccgctccggc
    13561 tgccagaccg aggagccgga cgactggctc tcggacggct acccgtggga gaccgcggtg
    13621 ccgagcgaag ccgtggaggt ccgcttcggc ggcaccgtgg accggcgcga ggagaacggc
    13681 cgcatggtgt atgagctgcg cgattaccac agcgttctcg ccacgcctta cgacgtgccc
    13741 atcctcggct acggcgcgca cgcggtgaac aatctccgtc tctggcgtgc gggcccggtg
    13801 cacaagcagc tcgatctcga cgctttcaac gccggagact attcccgcgc gcaaaaggcc
    13861 aacaacgacg ccgaggcgat cacctgcctt ctctaccccg acgacaacac ccccgccggg
    13921 cagcagctgc gtctcctgca ggaatatttc ttcgtctgcg ccgggctgca gagcatgctg
    13981 cgcacgttcc gcgccacgca tcttccctgg gacaagctgc ccgagcgcgt cgccatccac
    14041 acgaacgaca cccatcccgc gctctgcgtg ccggagctga tgcgtctttt gatggacggc
    14101 gagggtctct cgtgggagga ggcgtgggac atcacctgcc gcacgatctc ctacacgaac
    14161 cacaccatca tgccggaagc gctcgaaaag tggccggtgt cgctcatgcg ctctcttctg
    14221 ccccgccagt tccagatcat cgaggagatc gaccgccgct ggcgcgagag ctttgaccag
    14281 tccgttcccg gctggcacga ccgtcagcag gcgaccgcca tcatcggcgg cggcttcgtg
    14341 cgcatggcaa atctctccgt gatcggcggc cactcggtaa acggcgtggc gaagctccat
    14401 acggagattt tgaaaaacac cgtcctgcgc gatttttacg ccatcgcccc ggagaaattc
    14461 aacaacaaga cgaacggcgt ttcgcaccgc cgcttccttt tgcagtcgaa ccccggcctc
    14521 tcgcgctaca tcgccgagcg cgtcggcgac ggctgggtcg agcagccgga aaagctcgaa
    14581 gggcttctcg cctatgcgga cgataccgag tcgctgcgcg agcttgccaa ggtgaagctt
    14641 gcggcgaaaa agaacctctg cgattatatc gcgcgcgact tcggcattgc gtgcaacccg
    14701 gacggcgtca tcgacgtgca ggtcaagcgc atccacgcct ataagcgtca gctcctgaac
    14761 gcttttaaga tcctcgtgct ctacgacnnn nnnnnnngcg cacagctacg cttttgccaa
    14821 ggagagcatc cgctttgtgt gcgccgtggc cgatctcgtg aacgccgacc cggtggtgag
    14881 ccgccggatg aaggtcgtgt tcatggaaaa cttcggcgtc accatggggc agcgcatcta
    14941 ccccgccgcc gacatttccg agcagatctc caccgccgga aaggaagcga gcggcaccgg
    15001 ctgcatgaag tttatgatga acggcgccgt gacgctcggc acgctcgacg gcgcgaacgt
    15061 cgaaattctc gagcgcgtcg gcgcggagaa catttcgatc ttcggcctta cgagcgagga
    15121 gaccgagcgc ttcaccacgc agggcggcta caacgcccgc gaatgcgccg agagcgatcc
    15181 ggtactcgcg cgcgtgctcg cccacctcac ggacggctcg ctcggcagca agttctggga
    15241 tcttgaggac gcgctgctca aatataacga cgagttcttc gtcctgcgcg attttacgcc
    15301 gtatctcgcg gcgtgggacg ccctcgccgg agagaccggc aatctctcct tccagcgcaa
    15361 ggcgctcgtg aacatcgcca agtccggctg gttttccagc gaccgcgccg tcgccgagta
    15421 tgtgagcgac atctggaaga tccgctgaaa gcgggtgggg cagagccccg cccctacggg
    15481 agcttcccga tatcttcctg taggggaggg gctctgctcc tccctctcgg agaagccctt
    15541 tcattccccc gtttgttaag tccattacga tatacttagg gaggtacttg tccatgcgaa
    15601 aaaaagaatg cgtagccatg ctcctggcgg gcggtcaggg cagccgtctg ggtgttctga
    15661 cggagaagat cgccaagcct gccgtttcgt tcggcgggaa ataccgcatg atcgattttt
    15721 ccctttccaa ctgcgtcaac tccggcattg acaccgtcgg cgtgctgatc cagtataagc
    15781 cgtcgctgct caaccggtat ctcggcacag gcgcggcgtg ggatctggac gcggcctggg
    15841 gcggcgtgca catcctcccg ccgtatgcca cgcagaccgg cggcgaatgg tatgaaggca
    15901 ccgccgacgc gatttaccac aacatcgact ttctcgacgg ctacgacccc gagtatgtca
    15961 tcatcctctc cggcgaccat ctctaccaga tggactacaa cctgatgctc gacttccaca
    16021 agctcatggg cgcggatctc accgttgcgg tcaagaccgt gccgtgggag gaggccagcc
    16081 gcttcggcat catgtccgtc gatcaggaca tgcgcatcac gcgctttgcc gaaaagccga
    16141 agcagccgga gagcaatctc gcctccatgg gcatttacat tttcacctgg ccggtgctgc
    16201 gcgcggcgct gctcgccgac cacgccgatc ccgaaagcga caaggacttc ggcaagaaca
    16261 tcatcccgac gctgctcggc cagggcaaaa acctcttcgc ctatcagttc tccggctact
    16321 ggaaggacgt cggcacgatc ccgagctact attccacgca gatggagctg ctcgacgaaa
    16381 acgccgagtt ccgtctgcac gacacgcgca tgcatatcct ctccaacttc aacgagcacc
    16441 cctcccattt catcggccgg gatggccgcg tggagcacag catgatctgc aacggctgcg
    16501 agatctacgg cgaggtatac aattccatcc tctcccccgg cgtcacggtg gaaaagggcg
    16561 cggtggtgcg cgactccatc ctgcttccgg aatcgaccgt cggcgagggc gcgtgcgttg
    16621 agcgcacgat cctcaacgaa aacgcctcca tcgccgccga tgcggccgtc ggagcgccgg
    16681 gcaagatcac cgtcatcggc gagaacgccg tgatggaagt ggacgtttaa tttaagggag
    16741 ggaacattat gaaccgcacg atcggcctta tttccaccaa ctattccgtt cccggcttcg
    16801 gctccgttac cgaggtgcgc ccggcagcga gcatcccgtt cggcgggcgc taccgcctgc
    16861 tcgactttgc tttgagcaac atggtgaact cccgcgtcca gacggtggga ctcgtcacgc
    16921 cgtatttcta ccgcagcatc atggatcacg tcggcgcggg taagccgtgg gatctctcc
//
LOCUS       DAWAAE010000026        16787 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1554_length_16787_cov_2.887999, whole genome shotgun sequence.
ACCESSION   DAWAAE010000026 DAWAAE010000000
VERSION     DAWAAE010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 16787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16787
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1554_length_16787_cov_2.887999"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2041..2050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6810..6819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9733..9742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11432..11441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3212 a         5235 c         5072 g         3228 t
ORIGIN      
        1 cacgcagtcc acctccagat cgcggcagcg gtatacgccc gtgcggccga cctcgccgcc
       61 cgctgcggtg cggacgtggc ggagggcggc gcggatgcgc gcgagaagct cgccggggcc
      121 gaacggcttc gtgagataat cgtccgcgcc catgtcgagc gcgatgacct tatcccgctc
      181 gcgtcctcgg gcggagacga cgatcaccgg cagcgtggac cacttgcgga gccccgcaag
      241 gatctccatg ccgtccatat ccggcagact gagatcgagc agcacaaggt ccgggcagtg
      301 cgagctgatg agcgtcaccg cctcccggcc aaggctcgcg cggatggtgt caaagccgtt
      361 ggcttccagc accgctatga gaaacgcggc gatggtctcg tcgtcctcta cgatcaggat
      421 cttgtctttc gtctccatgg ctgtctcctt tgccggtttc aatgtccgga tgatatttct
      481 ttattctaca gcggtctcgc tctccggcgg caccggaagc gagaagctca ccaccgtccc
      541 gccgtcggta ttgcgggcgc tcatgctgcc gccgtgcgcc ttcacgatgg acatgcacac
      601 cgaaaggccg atgcccatct ggctgcggcc gccgacgccg tttttctccg gcagaaagcc
      661 gatcccgtcg aagagatgcg gcagcctttc gcgcacgatg ccggtgccgt tatcctgcac
      721 ggtgaacacc gcgacgttcc cctgccgcgc gagcgagacc gtgatttccg ttgcgccgcc
      781 gtggcgggcg gagttttcca gaaggttcgt gagcacctgc tggatgagca cggggtccat
      841 cggcacgagc atcaccgatt cgggcagctg cggcgtgacc gcaacgcccg gaaaatgcgc
      901 tttgaatttg gaaatggcgg agaagatgac ctcctcggcg acttcgtcct gcttttcgat
      961 gcgcgccgat tccaggccga tgcgcgttac ggaaaggaga ttttccacca tgcggatgag
     1021 ccattcggcg tcgtcgttga tgctgccgag aagcttcttt ttctgccagt ccggaaggtt
     1081 gtcgttttcc agaagcgtga tcgccgagcc ggagatcgcc gtaaggggcg tgcgcagatc
     1141 gtgcgaaatc gcccgcagga gattggcgcg catgcgttcg gtctctgcct ccatgcggat
     1201 gcgctcctgc cgcttgagcg tcgccgtcag cgtgttgacc gtgagcgaaa cggcgagcat
     1261 cgtgaggaac gtgagcggat agccggacag ggaaaagtta aagttcatat acggataggt
     1321 gaaaacatag ttcaccgcaa aaacgctcag aagcgagacg accgtgccgc agagataccc
     1381 gtccgtcagc agtgacacga gcagtacgca gagcacatac accagcggaa catacgtttc
     1441 gtttcgcagt tggctgatct cattcaagcc aaggcgctgc aaaagtgcgc atacgacggt
     1501 cgtcagagca aaaaggcccg ccgtgatccc ggcactcttc cacgtgaccg tccagttttc
     1561 cttcttcagc acggcagatc acctctgttt ctattgtagc atagaacgga taaagaattt
     1621 gttaggattt tcacaatgtc agcgcgtccg cggtacagac aaggaagcac tccctgtagc
     1681 tttgcgaaag gcgctcgtac gcggcggcag ccgcggcgcg atcgtcaaat acgccgaaca
     1741 ccgcgctgcc cgtgccggtc atcaccgcgc cgagagcgcc gccgtcaaga agtgcgccgc
     1801 ggatcgagcc gatctccgcg tcgctgcgcg gcagcgcctc ctcaaacaca ttgaacatcc
     1861 gccgcgcaac gccgcaaaga tccccggcgt gcagcgcagc gcacagcccc tgggtatccg
     1921 ggtgcgccgt gatgcggcgc ttatcgatgc gcccgaagag ctccgccgtg gagacggaaa
     1981 acggcggctt gcatatcacg atccatccgc ccggaagctc cggcagcggc gagagtattt
     2041 nnnnnnnnnn cctcggcgag catcatgccg ccggtgacgc agtacggcac atccgagccg
     2101 accgttaacg caagggcgcg aagctgttcc gcggagaaat tcgctccggt gagcacattg
     2161 agcgcgcgga gcaccgccgc ggcgtccgtg ctgccgccgg ccatgcccgc gccgaccggt
     2221 actctctttt ttatgcggat gtccgcgccg agagttttgt cgcccgcagc gtcaaagaac
     2281 acgcgcgccg cgcgcacggc aagattccgc tcatcccgcg gcagccacgg gatcgaacac
     2341 tttgcctcga tccgcccgtc gcggcggagg gtcacgtcaa catcgtccca gagcgagacg
     2401 ctgcccatga ccatggtgag atcgtggtag ccgtccgggc gtctggcgtg gacgtcgaga
     2461 gaatagttca gcttggcata gcctttttcg tttaccgtca cggaaaacac ctcatttcca
     2521 tacttcgcat tttaccgtat ttggtttctc attgcaacat ttccggcttt ctcccgtttt
     2581 tttacatttt gttcgcacac ggtgttccat cttcggaata attggtatat aatagtaatt
     2641 tagaagaata ggcaattctt gccgtacata cagggaggct gcaaatatgg caaaaaaagt
     2701 actggtagtt gaggacgaca gcaacatcgc ggagctgctg cgcctgtatc tggaaaagga
     2761 cgggttcgac gtgtttctcg ccgccgacgg cggagagggc atccgccgcg cgcaggagct
     2821 gcagccggat ctcatcctgc tcgacatcat gctgccggtc gtggacggct gggtcgtctg
     2881 ctccgaggtc cggaaaacct ccaatgtgcc gatcatcatg ctgaccgcga agggcgaaac
     2941 gttcgacaag atctccggac ttgaaatggg cgcggacgat tacgtcacga agccgtttga
     3001 ggtcaaggag ctgatggcgc gcatccacgc cgtcatgcgc cgcagcgagc cggaagccgc
     3061 gccgcacgcg gcggaaaagc ggcttgtatt cgataagctc gtgatcgacc ttgattccta
     3121 tgagctcatc gtagacggta agcgcgtgga agctcccccc aaggagatgg agcttctcta
     3181 tcatctcgcc tcctccccca accgcgtttt cacccgcaac cagctgctcg acgaggtgtg
     3241 gggctttgat tatttcggcg attcccgcac cgtggacgtc cacatcaagc gtctgcgcga
     3301 aaagctggag ggagtttcgg ataagtggtc gctcaaaacg gtctggggcg tcgggtacaa
     3361 gttcgagtcg gtcgaataaa cgatgcacag aacgtatttc cggaccttca ccacgaccgc
     3421 agcgatggtc cttgtgagct ttctgctcat cggcgcagcc ttcggctttg cgagccgtca
     3481 ggtgttcgtg tccgaagcgc gcgagagcat gatgacgaac tcacgcgaaa tatcgcgcat
     3541 ggcggtcgcc tatgccgccg agggcgatct gcgctcgctc gagctgcgca tgacgctcag
     3601 caccatcggc gcgagcacga atcagcacat tttcatcgtc aatccctccg gatatatcat
     3661 cacctgctcg gactccgccg ttttctgcga gcacatcgga aagcgtttgg atgagagcgt
     3721 aaaggcgatc ctgccgcagt ccggcacggt cgcgtcgctc ggcacgctgg gcggctttta
     3781 cgcgagcgag cattataccg tcatttccgc gctcaccgat gacggcgcgc tgctcggcca
     3841 tctgttcgtt tccaaggaca cggtaaccgc gctctccatc tgggagacaa tcctgccgct
     3901 gttcttcttt atcagtctca tcgtactctg tctcgcactc attttcagct acgcgagctc
     3961 caagtacttt gccaagcccc tgcgcgacat ggcggacgcg gcgcggcgct tcggccgcgg
     4021 cgagctgaac gtccgtgtgg agccgaccga cagcaccgac gagatcggcg agctgacgga
     4081 ggccttcaac accatggcgg actcgctcga gcagtcggag acgaagcggc gcgagtttat
     4141 cgcgaacgta tcacacgagc tcaaaacgcc gatgacgacg atctccggct ttgccgacgg
     4201 cattctcgac gggacgatcc cgccggagtc ggaaaagaaa tatcttcaga tcatctcctc
     4261 ggagacgaag cggctggccc ggctcgtgcg cagcatgctg gagctttcgc gcctgcaggc
     4321 aaaggaccgc gcaacgctcc tcaagaacag ctttgatatt tccgagcagc tccggctcac
     4381 gctcatcaca tttgccgata agatcgatca gaagcatctg gaggtcgtgt tcaacgtccc
     4441 cgaggaggcg atcaaagttc tcggcgatgt ggacgccatc acgcaggtca tctacaatct
     4501 gctcgataac gccatcaaat tctcccgcga aaattcacca ctcaccattt cgctatggaa
     4561 ggacagcaag aaggcctacg tctccatccg gaactgcggc agcaccattc cggaggcgga
     4621 gatcccgctc ctcttcgatc gcttccacaa gtccgaccgc agccgcagcc aggaccgcga
     4681 cggcgtcggt ctcgggcttt atatcgtcaa aaccattttg aacaaccacg gcgaggacat
     4741 tgccgtgaca agccgccagg gtgtgaccga ctttgtgttc acgctcgcgc tcaaaccgta
     4801 aaaggcggaa aggagtttta tggaaaatca ggaatctatg tggtatgccc cctctggcga
     4861 cacgccgcag gcatccccgc cggtcctcgt ccctccggcg aagccaaaga aaaaaatgcc
     4921 cgcgtggggc attgcgctga tccttgtcgc cgtggcggtg ctcatcatcg gctcgtgtct
     4981 gctcttccgg aacagcgccg cggcagaccc caaccacccc ggcgcggcgg acgatctgcc
     5041 gaacgattat aaatcgttct tcgataatta cttcaccgcg aacgaggaag cggaagaatg
     5101 tactatcccg accgtgcaga cgaccgagcg cgtgaccgtg aagctgtccc ccaccggtaa
     5161 aacgccgctt tccgcacagg aggtctatga gcgctgcgtt ccctccatcg ttgcggtgtc
     5221 ggcctttccg gatgagacgt cggaagacag ctattactgg ggctccggcg ttgttctgac
     5281 ggaggacggg tatattctca cgaattcgca cgttgtggag ggcacctgcc gcgcgcgcat
     5341 cacgctctgg gacgatacgg agtatgatgc tctgctcgtc ggctacgacc accgcaccga
     5401 tatcgccgtt ttgaagatcg atgcacacgg tctcacgccc gcggagttca cttcgaccga
     5461 ctctctgctc gtcggcgatg cggtatacgc catcggcaac ccgctcggca aggaattccg
     5521 cagcacgatg accgagggca tcatctccgg catcaaccgt gatatttccc acaacggcgt
     5581 gacgaacagc ctgctccaga cctccgcgcc gatcaatgag ggcaattccg gcggcgcgct
     5641 cgtcaacgtt tacgggcagg tcgtcggcgt caccaatatg aaaatgagca gccagtatat
     5701 cggctcggtg tccattgagg gcgtggcgtt cgccatcccg tcgagcaccg tgaagaccat
     5761 tgcggacagt ctgctcgaat ccggcgaggt acgcggacgc ccggcgctcg ggctcaccgt
     5821 cggcaagata ccgcaggcgg cgatggagga atacgatctc cccgccggcc tttacgtgag
     5881 cgctgtttcc gaaggctcgg actgcgccgc caaaggcatt gtgaccggcg acgtgcttct
     5941 tgccgcgaac ggtgtggagc ttaccgtcaa cgaggatctc accggtatta tcgccccgct
     6001 ccgcgtcggc gatgagctta cgctcaccgt gtggcacgag ggcgagacac gggacattac
     6061 cgtgaagctc gtggacgtga acgatgttta ctgatataga aagggatata accatgaacg
     6121 aaaagatcat tgaaaacttt ctttctctct gcgccatccc gcacaagtcc catcacgagg
     6181 aaaagatcag ccggtttctc tgcgactggg ccgtgaaaaa gggtctcgcc gccgtgcgcg
     6241 acgaggaagg caacgtcatc atcgataagg ccggttccgc cgggcacgag aacgcgccgc
     6301 gcaccattct gcaggcgcat atggatatgg tcgtcgtctg ggaggatggc atggactttg
     6361 atccgctcga cgataccatc accgtcatca acgacggcaa aacgctcaag gccaaaggca
     6421 catccctcgg cggcgacgac ggcgcgggcg ttgcacttgc gatgtccatt cttgaggatg
     6481 agaccgccgt gcacggcccg atccgcgcga tcttcaccgt ggacgaggaa gacggcatgt
     6541 ccggcgcgga ccatctcgat ccgaagcacc tcgacgccaa atacctcatc aatctcgact
     6601 gggaggagtt cggctcgctc tgcaattcga gcgcgggcag cgatatgtac cgcatgcacc
     6661 gcgccgccga gtgggaagcc gcttccggca aagcgtataa gatcgccgtt tccggtctcg
     6721 tcggcggtca ctccggcgct tccatccatc tgaagctcgc caacgccatc cgcattgccg
     6781 cgcgcacggc aaaaaccgcg ctcgacatgn nnnnnnnnnc cgcggcggta gcgcccacaa
     6841 tgccatcccc tcctcctgca cagccgtgat caccgtgcca gaaaaggacg ccgaaaaggt
     6901 tctcgaagcg ctcaacgccc atgcgcttac cgaggcggtc gcctgcgctg cgaccgatcc
     6961 ggaggcgaag gtcgccgtaa ccgctgccga gctccccgaa aaagcgctca cgggtgagct
     7021 ttcgcgcggc tatctcgcgc tgctcaccga ggtgttcgac ggtgtgcaca ccatgtcgcc
     7081 gtccatcccg tcgctcgtgg agagctcggc gaacaccggc cttgctgccg tcggcgagga
     7141 cgaagtcgaa ttcaccgtcc atcagcgcag cagcgaaccc gccatcaccg cggaaatgaa
     7201 gaaggaatac gagcgtcagg catccgagca cagctttacg atgacgctgc tcagctcctc
     7261 ccccgcgtgg ccgctcaagc ccggcagcga gcttgtcggc atctgcgagc gcattttcaa
     7321 ggagcagaac ggacacgaca tgtccgtaga accggtgcac gccggtctgg agtgcggcag
     7381 cttctcttcc gccaatcccg cgctcgacat catctccatc ggccccgaca ttctggacat
     7441 tcattctccg cgcgaaacgc tcgtgctgga taccgtgtat ggctgcgata cgctcatccg
     7501 cgccattttg aaggagatcg ctgacaagaa agcatgaaga cctacgacgc cggaaaattt
     7561 gatattgccg tcatcggcgc cggacacgcg ggcatcgagg ccgcgctcgc cgcggcccgg
     7621 ctcggtctgc gcaccgtgtg cttcacggtg aatctcgaca gcgtcggcaa catgccgtgc
     7681 aaccccgcca tcggcggcac gggcaagggg caccttgtcc gtgagctcga cgcgctcggc
     7741 ggcgagatgg gcaaagcggc ggacgccgcg ggcatccagt tccggatgct caaccgcggc
     7801 aaaggccccg ccgtctactc tctgcgcgtg caggccgacc ggcggcgcta tcaggagatc
     7861 atgaaggaga cgctggagac gcaggaaaat ctcgtgctgc atcaggcgga gatcgtttcc
     7921 atcggcctta cggacggccg tgtttcctcc gtcacgacgc acacgggcgc cgtatacgcg
     7981 gtgcgcgctg ttgtcatcgc tacggggaca tatctcggcg ggcggacgat cgtcggcgac
     8041 gtggtacgca cctccggccc ggacgggctc gccgcggcga cggagctcac gaagtgcctc
     8101 gccgatatgg gtcttcgtct ccgtcgcttc aagaccggca cgccgccgcg cgtgaacgcg
     8161 cgcagcgtcc gctttgacga aatgcagctc cagcccggcg atgaggagat cgagccgttt
     8221 tcctattcca ccgacgctgc ggcgctgcat aaccgcgccg tgtgctggct tacctatacc
     8281 aaagcgcgca cgcacgagat catccgcgcc aatcttgacc gcagtccgct cttcaacggc
     8341 accattgaaa gcaccggccc gcgctactgc ccctcgattg agacgaagat cgtcacgttt
     8401 ccggataagg aacgccacca gctcttcatt gagccgatgg gtctgcatac gcaggagctg
     8461 tacattcagg gtctgtcttc ctctctgccg gaggacgtgc agatcgatgt tgtccgctcc
     8521 attccgggac tggaaaacgc cgagatgcag cgctgcgcct atgccatcga gtacgactgc
     8581 atcgatccca cggagctgcg cgccacgctc gagagcaaaa agatccccgg tctgtacggc
     8641 gcgggccagt tcaacggctc gtccggctac gaggaagccg ccgtgcaggg attcgtcgcg
     8701 ggcgtgaacg ccgcgctcaa gatccgcggc gaggagcctt tgatcctgcg ccgttcggac
     8761 ggctacatcg gtacgctcat cgacgatctc gtcaccaaag gcaccgaaga gccgtaccgt
     8821 attatgacgt cgcgcagcga ataccgtctg ctccaccggc aggataacgc cgacgagcgg
     8881 ctgacgcaca tcggccaccg catcggactc atttccgacg agcggctcgc cgccgtgcag
     8941 gacaaatacg ccgccgtcgc aaaggagcgg cagcggctcg agcatacgca tctcgccccc
     9001 ggcgaggcga tcaacgcgct gctgcgcgaa aaaggcacgg cagagatcgt ctccggcgcg
     9061 tcgcttgccg atcttctgcg ccgcccgcag atcgaatatg ccgatcttct cccgtttgac
     9121 ccggagcgcc cggcgctgcc gcgcgccgtc ggcgaggagg tctccatcac gctcaaatac
     9181 gagggctata tccgccgcca gctcaagcag gtggaggatt tccggcgcat ggaggatcgt
     9241 cctctccccg aggatatcga ctatgccgcc gtgccggggc tccgcaccga ggcgcggcag
     9301 aagctcgtcg ccatacgccc cgtgagcttc gggcaggcct cgcgcatttc cggcgtgtct
     9361 ccggcggata tgaccgcgct tatgatctac accgagagcg gaaaggagcg ccgccatgga
     9421 taaggtcgtt ctcggtctct ccggcggcgt ggattccgcc gtggccgcgc acctgctgcg
     9481 cgaatcgagg ctagaggttt acgggctgta tctcgataac ggtctccccg gcgcggatgc
     9541 cgcgcgcgag gccgccgctt ccctctccgt tccgctcacg atcgccgaca gccgcgaggc
     9601 aatggagcaa tacgtctgca ccccgtttgc cgaagcgtat ctccgcggcg agacgccgaa
     9661 cccctgcatt ctctgcaacc cggcggtcaa gttccgcgcc ctgctggacg aagcggagcg
     9721 cagagcatac acnnnnnnnn nncggagcgc atcggggcaa agtacatcgc caccgggcat
     9781 tatgccagag cggaaaacgg cgccatttac cgcgggcagc cggaaaatga ccagagctat
     9841 atgctctgcc gtctgacgcc ggacgaggtg aacgcccttc tgctgccgct tgcgccatat
     9901 aaaaaaacgg aggtgcgcgc gctggccgaa gagctcggcc tgcccgtcgc gcacaagccg
     9961 gacagcatgg acatctgctt catcccggac ggcgattacg ccgcctggat cgaacgccgc
    10021 ggcatcacgc ccccgccggg ggatttcgtt ctggacggta aaaccgtcgg caaacaccgc
    10081 ggcatccacc actataccgt cggccagcgc cgtcacttcg gcgtcggctt cgggaaacgc
    10141 gtatacgttt ccgacatctg cccggagacg aacgaagtcc gtctttcttc tgatgacgcc
    10201 gccgtatggc gggagagttt ccgtgtgcgc ggcgtaaact ggcttgttcc accggagggc
    10261 gagatcgtct gcggcgttcg catccgccat tcacgccgcg agctgcccac cgggacggtg
    10321 acgccggacg gagacggcgc aatcgtccgc ttccctgcgc cggtacgcgc ccccactccc
    10381 ggccagaccg ccgcctttta cgacggcgac cgtctgctcg gcggcggatt tatcgtaaaa
    10441 tagcaaaaga gagggctctt cgccctttct ttttgttttt tctgtgcatt tactttagca
    10501 tggtagcgtg ctaatatact accataataa atcaagtaca ggagagacct accatgaaaa
    10561 aaattcttac catcgcactt accctttgtc tgctcgtaac ggcgctggcc ggctgcggca
    10621 gcagcgcccg ccgctcccga tccgaccgcc gcccccacgg aagctccggc ggaagccacg
    10681 gcagaacccg ccgccgaggg cggcaaactg atcgtcggct ttgatgccga gttccccccg
    10741 ttcggctaca ttgccgagga cggcagctac gacggctttg atctcgcgct cgcgcaggag
    10801 gtctgcgcgc ggctcggctg ggaatatgaa gctgtggcga tcgactgggc gtcgaaggac
    10861 gccgagctca aagccggaaa catcaactgc atctggaacg gcttcacctg caccggccgc
    10921 gaggaagagt acacctggtc ggacgcctac atcgataaca gcatcgtcgc cattgtccgc
    10981 gccgactccg gcatcgagtc cctcgccgat ctcgccggaa agaccgttat ggtgcagtcc
    11041 gcttcctccg gtctggacgc tctcaccgcg aacgaggagc tgaccgcctc cttcggtgag
    11101 ctcgtgcagc tgcccgacta caacaccggc ttcctggagc tgcggcaggg tacggtggac
    11161 gccgttgtcg tggacgtcgg cgtcggcaac taccagatcg ccaacaccga gggcgactac
    11221 cttatcctct ctgagcccgt ctccgtggag cagtacgccg tcggcttcta tctcggcaac
    11281 gaggcgctgc gcgacgccgt gaacgagcag ctgctcgcca tggcagagga cggcacgatg
    11341 atgagcatcg ccgagaagta tgttgacaag ggactttctc tcgacagtct ctgcctctgc
    11401 aagtgatact tccctcgaaa tgatatcccc gnnnnnnnnn ngcgccaacc tgtctacgga
    11461 aaggatcgat ccgatgagca ttcaaaccat gctggtctct ctctcccagg cgtttctcac
    11521 aacgctcgcc atattcttcc tcacgcttct cggctcgctg ccgctcggtc ttgtcgttta
    11581 ctttgcgcgc aaaagccact tttcgccgct ccgttggctc acgaaggtgt atatcgccat
    11641 tatgcgcggc acgccgctca tgctgcaggt catggtcgtg tactatgggc cgagtcttct
    11701 tctcggcatg acgctgccgc ggaactggcg ctttgccgcc gtgatcgtcg cgttcgtcat
    11761 caactatgcc gcgtatttcg ccgagatcta ccgcggcggc attgagtcga tcagcgccgg
    11821 acagtatgaa gcggcgcagg tgctcggcta ccgaaagggg cagacgttct tccgcatcat
    11881 ccttccgcag atggtaaagc gcgtgctgcc gagcgtcacg aacgaggtca ttacccttgt
    11941 aaaggacacg tctctcgcct ccgtcatcgg cacggtagag atgttcacgc gtgccaagca
    12001 gatcgccgtt gcgccaaatt cgccgggcat gctggcgttt gccgccgctg cggtcttcta
    12061 tttcgtattc aacagccttg tcgccttcgc catggagcgg atcgaaaagg cgttcgccta
    12121 ctaccgttga aggagggccg gacatgaaca cattggaact gcgcaacatc cgcaaaagct
    12181 ttgacgggca gggcgttctc gaagacatct ccctttctgt cccggcgggg agcgttacgg
    12241 cgatcatcgg gccgtccggc agcggcaaat ccacgctgct ccgatgcgca acgctgctcg
    12301 agaccgccga cggcggcgac atcatttatg aaggaaaata tgccgcgaag gacggcgttt
    12361 acgcgccgaa ggccgagctg cacgccatcc gccgctattt cggtctcgta ttccagagct
    12421 ataacctctt cccccacttc acgcttttga agaacgtgat ggacgcgccc gtgcaggtgc
    12481 agaagcgccc cgccgccgag gtacgcgagg aagccatggc actgctcgga aaaatgggtc
    12541 ttgccgatcg cgccgacgcc tacccctgcc agctttccgg cgggcagcag cagcgcgcgg
    12601 cgatcgcgcg cgcgctttgc atgaagccgg agctgctgtt ctttgacgag ccgacgagcg
    12661 cgcttgaccc ggagctgacc ggcgaggtgc tgcgcgttat ccgctcgctc gccgaagaaa
    12721 agatgacgat ggtgatcgtc acgcacgaaa tgcagttcgc ccgcatggtc gccgaccgcg
    12781 tcgtattcat ggacgggggc tgcatcgtgg aggagggcac gcccgaacag gtcatcgatg
    12841 cgccgcagca ggagcgcacg cgcgcctttc tgcgccggtt ggaacggtaa tttcctccgg
    12901 ggcttgattt tctcaccgga atccgctatc ataaaatccg cctttacata atccatgtaa
    12961 aatggcggat ttttttgttg ggaagggtcg aacttatgaa gaagatgcgg agatttctgg
    13021 cgttcgcgct gtgcgcggcg ctgtgttttc tgcttttgac gggttgcggt tcgcgcgaca
    13081 ccatccggtt taaaatgatc gcggagctca accaggagga attctgcgtc gccttccgca
    13141 agggcgaccc gctctgcgat atattcaccg ccgcgctcaa ggagctctcc gccgacggac
    13201 agatctcccg gctgagcgcg cagtatctcg gcacggacta tacctgcatg gaggcgctgc
    13261 ccggcgcgct gcagcttctg gaaacgcagc cggagccggg acgcaagctg cgtatcggcg
    13321 tgcaggacgg tattgctccg atctcctcct cccgcgacga cggctccttc ggcgggctga
    13381 tcccggatct tgcgcagctc gtggcggaaa agctcgggtg gacgtttgag tatatggtca
    13441 tcaacagcga caacgtcgcc gccgagctcg gcagcggcaa tatcgactgc gcgtggatgg
    13501 cggcatcctt ttccggcagc tcaagctccg tctccctctc ccccggctgg cttcgcaaca
    13561 cgcatgagct cgttgtgcgc agtgacagcc gctatacgcg caaaaacagt ctcaagggca
    13621 aggtgatcgg catcaccgac gccaccgcgc tcgccgcgct caaggaaagc ggaatggacg
    13681 cgaaagtcgg ctcggtctgg tactacgacg atctttccgc ctgctttgcg gcgctcgccg
    13741 ccggggactg cgacgccgtg gtcatcgacg gcatcgtgtc cgggtattat atgtaacttc
    13801 gttttatagt gaaagcgctc tgcacaatgc agagcgcttt tccatactat gaagcgaggc
    13861 gcccctcaaa gccgcaggca gaaagggggg aatccatgaa acgctgctgg aaggactggc
    13921 tgcgaaaggc gggcatccgt gcgctgaaga ccgtggcgca gacggctctc gccgctatcg
    13981 gcacatctgc tgtgatctcg gagatcaact ggctgatggt cggcagcaca tcgctgatcg
    14041 ccggcgttct ttcactgctc acatcccttg ccgggatccc ggaggaatgc ccggaggaga
    14101 tcgaagcgac gaagccgccg gaaagttaag ccgaacagac aaaagccccg ccgggtatcc
    14161 cggcggggcg attttggctg tttctgcgat caggcgtact gatcgacgac cttggcggcg
    14221 cggtcggtgt cggcgcagac aacggcgatg acatagtttc cgatcgtctt taccggcgcg
    14281 ttttcgagcg acatggcagc ggcggggccg tagctcttat aggcttcgat ccggtcggcg
    14341 acgaacgtct ggagcgcggt gagcaggcgc tgcgcggcag cagcgtccac ggcctcgaag
    14401 cagacgatct gctcggcagt cgccgctgtg ccgcagtaga cgagcgcgct cttatagtcg
    14461 gcgctgtcca cgccgtagta ctgggggatc acggcgtcat ccagctggct gaggctgtcg
    14521 gtaaagttgg cgtttgcgag caggtcgtcc acgaacgcac gcggctccat cgtctttttt
    14581 gagctgccgc agccggtgag cacagcaaag aggcagcaga gcgcgagcag aagagaaatg
    14641 gtctttttca taaaattacc tcgtttctta ttcggtgaga aggccctcgt ccacgtagtg
    14701 ggtcttcatg taatttgccc aggcgacata cccggatttt tcaaagtgct tgccgtctcc
    14761 ggcgaaggca tagttgctcg aaaggtagcc gtactcgtcc acgagcatgg agtagatatc
    14821 gagataccag catttttcgt ctgcgcagag ctgcttgagc gcggagttga aggcgttgat
    14881 gcgatccatg ctctgcacct cgttgggatc ggcgtccact ttctgcgtga cgggtgtgac
    14941 ggagataaca tagatgttcg ccttcgtctg gtgctcacgg acgtactcga tgagcgtttt
    15001 atagctctgg acaaaggagt ttacgtccgt gccgacctcg ttcaggccaa acatgagata
    15061 aatgtcatga aagtaatccg cgtcgttgag cgtcagcggc gagagatcga gcgtgccgag
    15121 aacattgttc acgaccgcgc cggtgccgta aatgcagctc atgttcgtta cgcccgacca
    15181 caggcgaaag ccctcgacca gcgagtttcc gatcatgcag gagcggtcaa aatggctgtc
    15241 gtacaccgcg ttcgtacgct cgggcacgat cgcgccccag tcggcaggga cgatgagatc
    15301 gccgtcgcgc tgcgccttgg cccccgaagg cgtcggcgtc ggcgcggcgg tcggcttcgg
    15361 cgtcatggat acctcggcgg taacctccgg cgttgcttcg cccggctgcg gcgtgtctgc
    15421 cggacgcggc gtcgcggtca cgggcgcgta gctcggcagc gtgtacctcg gcttttcatt
    15481 gcccttccgc gcaagcgtca cgatgccgaa aatcaaaagg ccgacaagta caagcgcaag
    15541 gagcagaaac gccggatgaa tgcggttttt cccgatgcgc ggcagacgga aggaaaagcc
    15601 cttgccgccg ttatcggacg gcgggcggcg cggcggcgtg ccgccggaag agcgtcccgc
    15661 agcggcgcgc tgtgccgggc gacgaacggc gccggggcgg taataatccc cgtgcggacg
    15721 ctgggaagcc gcgtaagagc gcggcggcgt ttcccgctgc gggagactcc cgccgtaaaa
    15781 gggatcgctt tccgtttccg gcggctgcgg cgcggcgcgg tagccgccgg tcgcatacgg
    15841 ctggcggtag ctgccgtcgc tgcggcggta gggatcgttc ctctgctgcg gctgcgccgt
    15901 tctgccgtag ggatccgtct gcggctgtgc ggcgttataa tacggatcgg cctgctgcgg
    15961 ctgcgctgcc gggcggtaag cctctctctg cggctgtacc gggcgcccat aggggtcggt
    16021 ttgcggctgc gcgggcgcgg cgcggcggtt tccctgtgcg ctctgctcca gatcgccgct
    16081 gcggagagca tcccacgata tttccgtaaa ggctgcgctt cctccgccgt aaggctgctg
    16141 ccgctgctgc ggctggcggc tgccatagcc gttatagtcc gcatacccct gcgccggacg
    16201 gctctgcggc gtatacggca cagcgcgcgg cgctgttctc gtcggttcgg cggcggggcg
    16261 ggcgctgcgg cggtttgcgg tctcggtctc gctatattgg ggcgccgggc ggccggagtt
    16321 cggcgtgcgg cgcacgctgt cgagcggcag ctcggtaaaa agggcgctct agcgctggga
    16381 tgcagcgcct tcccgctgcg gcgggcgcga cgcgccgtct cttcgataat agctggattc
    16441 gcggttttcc atgtggaaag atacctccgc acaataatac aacgatatta tagtgaattc
    16501 tgtcttagta tgcaagtaaa aaaagtgttt cgttttcctt gcgcagcagc gcttggcgtg
    16561 gtatagtaat ttggttaaaa tagagttatt gtacgcagaa ggagttttcc catgtggatt
    16621 gccgataact ggaaagatta tgaactgatc gatgcctcaa acggcgagcg tctggagcgt
    16681 tgggggcagc acattctcgt gcggcccgac ccgcaggtca tctggaaaac gccgcgcacc
    16741 tcccccgtct ggaagcgccc ggaggggcgc tgcagccgca gccacac
//
LOCUS       DAWAAE010000027        16509 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1591_length_16509_cov_3.448159, whole genome shotgun sequence.
ACCESSION   DAWAAE010000027 DAWAAE010000000
VERSION     DAWAAE010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_16509_cov_3.448159"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1217..1226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3206..3215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14441..14450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3226 a         4916 c         5149 g         3188 t
ORIGIN      
        1 gccgcacccg cacgctcgag gaggtcggca aggagttcaa cgtcacgcgt gagcgtatcc
       61 gccagatcga ggcgaaggcg ctgcgcaagc tccgccaccc cagccgcagc aagaagctcc
      121 gcgacttttt gaactgagac ttcttttaag gaggaaaaac catgttccga cccgtgcgaa
      181 aaaagaaaaa tgagatcagc gcagaagcgg caaagcagct cctgcacacg gagcgccggg
      241 gcgttttcgc cgtgaacggc gacgacggct acccctacgc cgtcccggtc aacttcttct
      301 atgacgagga ggccaaccgg atctatttcc acggctcccg cgccggacac aagatcgact
      361 ccgtccgcaa gagcgacaag gtctgcttca ccgtgtacgg caacgagacg atccgggacg
      421 aagcctgggc gccgtatatg cagagcgccg tcgtgttcgg ccgctgccgc ctgccggagt
      481 acggcgagga gagcatggcg cttctcaagc gcttcgccat gaagtattac cccggggaga
      541 gcatggtgga cgccgagatc gccgacgccg gaagggccgc gcagatgtat gtgatcgaca
      601 ttgagcacat cagcggcaag gaagtgcagg agaagtaaaa cccgatacat aaaataaagg
      661 ggagttccgt tccgcgaagt tgccgcggag cggagctctc ttttctaaca cgttctttat
      721 gccgccggcg ggcgggatat gatacaatat tctgtcaaat cgcgtcgaac ggaaggagag
      781 agccgatgca gaccgatcgc ggaaagaaat ggaacgaaat acgcatgcgc cttttcaaaa
      841 tggtgtcggt gggcgtggtg gacgatccga tcaaccaggg gtacgacatc gtcagtacgc
      901 tgatgctgat cgtgaacctt atcggggcgt ttgcgaacac gttcgacggc gtgcgcgcgc
      961 agtacgcatc gctcctcgat ctcaccgagg agatcacggt cgccttcttc gcgctcgact
     1021 atgcgctgcg gctcatgacg gcaaagtacc tgtacccgca gctcaccgag gggcgctctc
     1081 tcaccaaata catgctgtcc gccgggggca tcgtcgatct tttgtcgttc ctgccgtttt
     1141 atctgccgtt ctttttcccg agcggcgcgg cggcgttccg gctgttccgc gtggcgcgca
     1201 ttttccgcct gttccgnnnn nnnnnngtat tacgattcgc tcaacgtcat caccgaggtc
     1261 atcgtcggca aaaagcagca gcttttatcg tcggtgttca tcatcctcgt gctgatgctc
     1321 ggctcgagcc tgtgcatgta ctcgcttgag cacgacgcgc agccggaggt gttcaaaaac
     1381 gccttttccg gcgtgtggtg gtcggtgtcg acgctgctca ccatcggcta cggcgacatt
     1441 tatcccatca cgcccgccgg aaaagcgatg ggaattttca tcacgttcct cggcgtcggc
     1501 atggtggcga tcccgaccgg tatcatatct gccggcttcg tggagcagta cacgcgcctc
     1561 aagcgtctcg gcgactatgc cgaggaagag gagctgcatt tcatcaagac cgagctgcac
     1621 cgcggcgatc tctgggtcgg gaagacggtg cgcgagctgc atctgccgca gggcgtgatc
     1681 ctcgcggtca tccagcgcgg cggcgatacg atcatccccc gcggcgacgt ggcgctcaag
     1741 gccggagacc ggctcgtcat cggcgccgag cacctgaagg gcgaccggcc ggtcaatctg
     1801 cgtgagatca cgctgcgcaa aaaccacgac tggaacggcg ttgccatcaa ggatatcgac
     1861 atttcccgcc agtcgttcat cgttatggtg cggcgcggga agaagctgat tgtaccctac
     1921 ggcaagctcg tcctcgccga gggcgacacc gtgatcctat acagcaagga agcgccgagg
     1981 ccgacgatca cattctgaaa caagaaaacg gcgggactga ccgaacgtca gccccgccgt
     2041 tttttacttg tgcgcgagaa gttctttgcc ggggatgccc ggctttgtga gattcatggg
     2101 atcgaggatc gtgtcgatct cctcttccgt gaggagcttt tcctgcagta tgagcttccg
     2161 gacgctctgt ccggtgacga tggcgcgctt ggcgatgtcc gcggcgtgct gatagccgat
     2221 gtgcggcgtg agcgccgtga tgacgccgac gctgctctcg acaagctccc ggcagcgctc
     2281 ctcgttcgcg gtgatgccgg tgacgcagtt gtccacgaac gtgtgcacgg cgtaggtcag
     2341 cgtgccgatc gactggaaca ggcagtagaa gatgatcggc tcaaaggcgt tgagctcgag
     2401 ctgcccgccc tcggcggcca tggtgacggt catgtcgttg ccgatgatga ggaacgccac
     2461 ctgattgatg acctccggaa tgaccgggtt caccttgccc ggcatgatgg acgagccgtt
     2521 ctgccgcgcc gggagattga tctcgccgaa gccggtgcgc ggcccggacg acatgagacg
     2581 aaggtcattg gcaattttcg agagcgtcac cgcgcacgtt ttgacagcgc ccgacaccgc
     2641 cacaaacggg tccgggttct gcgtggagtc gatgagatcg cccgcctgcg tgagctccat
     2701 gccggatacc tcgctcagca cggggacgat gcgccgcagg tacgtttcgt ccgcgttgat
     2761 gccggtgccg atggccgtgc cgcccatatt gagcgagcgc atctcgtcca tggccttgcc
     2821 catgcgcacg gtgtcgcgcc gcacggcgtc ggcataggcg cgaaactcct gccccagccg
     2881 gatcggcacg gcgtcctgca tctgcgtgcg gcccattttg atgacgtggt taaattcacg
     2941 ggatttgtcc tcaaacgcgg cacgcagccg gtccagctcg gtctccagct ttttcagaag
     3001 gcgcagcgtc gtcatctttc cggcggtggg aatgacatcg ttggtggact ggccgcagtt
     3061 gacgtggtcg ttcgggttga cgagcgagta gtcgcccttg acgccgccga gcaactcgat
     3121 ggcgcggttg gcgatgacct cgttggcgtt catgttgatg gatgtgccgg cgccgccctg
     3181 cacgggatcg acgatgaact gctcannnnn nnnnncgatg gcgtttgcga tacgctcgtt
     3241 cagcagtccg gcctcaaggt tcgtgagtgc cgccgccttt ttgatgtagg caagactgcg
     3301 gatgagctcc gggttcatcc gcagccccgt gatggggaag ttttccgcgg cgcgcagcga
     3361 ctgcacgcca taatatacgt ttgccggcac gtccttcgtg ccgatggagt ctttttctac
     3421 gcggtaatcg gaagtatgat tttccatttc tctttctcct gcgtctcctg tgatgaagtt
     3481 caatttgatt atagagcggg atataatata gtcaaatata aagtcaatac ttgtacccat
     3541 aacttcaaag ttatataatg gcggaaagag ggggcgacag catgaagcag agagtctttg
     3601 acggcatgga atacgtgtat gccgtgtacc gggaagggag cttccagaga gcggcggaga
     3661 agctgttcgt ttcgcagccg tccgtgagcg cgtcagtgcg ccgggtggaa gagcgcgttg
     3721 ggagccgcct gttcgaccgc agccggaagc cgctcgcgct caccgagtgc ggcgaggcgt
     3781 acatcgcctg cgcggagaag atcctcgcca tggagcggga ctttacggag tatgtgaacg
     3841 actgggaggg gcttcgccgc ggaaggctcg cggtcggggg gagcagcctt ttttcctcgc
     3901 tgctgctgcc gccgatgatg gcgtcgttcc gggagcgcta ccccggcatc acgctcacgc
     3961 tggcggagga gacgacgccc cggctcgagg agatgctgcg gcagggaacg gtcgatctcg
     4021 tggtggatta tacgatcccg aacatggagc tttatgattc cgcgaccgtc cgggaggacg
     4081 cgctcattct cgccgtgccg cgcgcggcgg cggtcaacgc gcgtctcgcg ccgtaccgca
     4141 ttgcaccgga agagatcggc gctgcggcgc gaagcgctgc gccgggcgtg ccgatggagc
     4201 ttttgaagga cgagagcttt gtgcttttga agcccgaaaa cgacacccgt gcccgggcgg
     4261 acgcgctctg ccgtctcggc ggctttgagc cgaaaacggc gatggaattt gaccagcaga
     4321 tgacggcgta tctcgccggg tgctccggcg cgggggtcac gttcgtgagc agcgtactcg
     4381 tatcgcgcct ctcgccgaac cccggcatct gctattaccg cctgccggag ccggagagcc
     4441 gccgggatat ccggctcttc tggaagcgcg gccggtataa gacccgcgcc atggaagcgt
     4501 ttctcgccat gccgtcggag aacggcgggg ggggataata agaaagccac cgaacgaacc
     4561 ggtggcttta actttatttt gcggctttgg ccttttcgta cagatccatc aaaaccttgc
     4621 cgatcttcat ggaatactcc acgaccagat gcttctggtc gttggggaga tagcggccga
     4681 attcctggat ctcataggtc agatcgccgt catagccgat atccgccagc ccggccatgg
     4741 cctcggccca gtcaatgttg ccgtggaagg gcatcaggtg ctcgtcggcc acgccgtggt
     4801 tatcctgcac atggatggcg tggagccggg aaccgatgat gttcagattc tgccggtgga
     4861 agtcatgtcc tcccagattg gcgtggccga agtcatagca cacgcccacg tgctccgggt
     4921 cgccgaaggc gtccacgaga tcgaccagct cgtaaatgct ggcgcagtag atgcgctgca
     4981 tgggctggcg gggacggtat tcaaagtcgt tttccagaca aatgccgatg ccgttttccc
     5041 gggcggtggc tacgtgccgg ctgtagtatt ccagattctt ttctttggag accgaaacgt
     5101 ccggcccggc ggaatacacg gtgccggggt gcgttaccgt ccactccacg cccagctcag
     5161 ccgaggcgat gatggagcgg cggatcagct cctccatggt tttgactttt tcttcgtcgt
     5221 tctcggcgaa gatgtcgtaa tagggcagat gggactggcg gaacacgacg ccccagcggc
     5281 gtcccatttc cgcgatgtcc ttcacatagt cctgccagtc gtcgtttcgc atgcgggagt
     5341 gcggattcat ggattcacag aagttgatgt ccaaaacctt atagccgccc tgggcgcact
     5401 gggcaatgca gaaatcaaag ccgttacgct cccgtccggg ggtaaaggca cagatgttgg
     5461 tagaagtaga gatctccata ttggcgctcc tttgcttttt tattcagggg cagcgccgcc
     5521 ggacgccggt gacggtgctg cgcggcgctg ccctaaaatc aaatggagaa gagggccgcg
     5581 aaagcggccc tttcttagaa aatcgggatc aggcgaccgc gttgttccgg gcgtactcgg
     5641 tcagcagatt ctgcatctcg tcggacatga tctctaccgt ttcctcgacg gtctgatcgt
     5701 tttccagcat gtcggacacg ccctgcatga tcgcatagta gaaatccttg gacgggccga
     5761 cggtaacgct gcgcatatcc gccggggtgg agatgagctg atcgtacgcc accagatact
     5821 gcggatactc cgccgtcacg gtcttataaa tatcctcgtc catagccgcc gtgttggagg
     5881 gaatgtagcc ggtgttggcg gcaaagtcgg cctgcaccgc cgcggaagcg agatactgca
     5941 tgaagaccca ggaggcttcc ttgcgaagat cgtcgccgga atcgaacatg gacaggcagg
     6001 agccggccac ggtggcgccg tcggaagcgt cggcggaaac gcgcagatac ttgccgacgc
     6061 cgacttcaaa ggcgccgttg accttttcga ggaccggggc cacgttggaa gaagaattgg
     6121 tcatcatcac gagatcgccg gcgatgaaca tatcggccga gccgctctcg ttgatgagcg
     6181 cgccggcgtc atacatggcc ttccactgcg tcaggaaggt ggccagcgcg ccgttgtcga
     6241 tgcaggcaag ctccgtggcg gtgccttccg agccgttctt ttcattgacg acgtagctgc
     6301 cgagctgacc cagccagttg gcgagcgtcg gggtgttcgg cacggtctgc agcaccttct
     6361 gggtgagacc cttggcctgc atgtcctgat aaagccggat aacatcgtca aacgtctcgg
     6421 ggaccttgtc ccagccggct tccttcagca ggtccatgtt gtagtaggaa acggtggtag
     6481 aggtcgcaaa cggcagacca agctggctgc cggcaaactg ccagttggcc agagcggcgg
     6541 agagataggt gtccaggcag ccgggctccg cgtaggcggc cacggcctga tccacggtga
     6601 aggctttgcc ggagttgtag taggtcattt tgcccgtggc gtccagctgc atcaggtcgg
     6661 gcagctcctc aaagttgccg ccggagatga tggccttgag cagggtgctg gcgtcggagt
     6721 actggccctg atacacggcg ttcacggtga tgccgtattc gttggtgctg ttgaattcct
     6781 caatgtactt gtccatcagc acgccggcct cgtccgaggc gcagtgccag aacgtgatgg
     6841 tcaccggctg cgtgccgagc aggctctcga ccggcgcggc ttcctcctcg gccggggccg
     6901 cggtgggctc ggccggcgtg gtctctcccg tgctgccgca ggcggccaga gagaaaacaa
     6961 gcgccagagc cagaagcaat gacagcatct ttttcatgtg taaatcctcc taaaatgatt
     7021 ttattccaga aaagcagggc gctgctgccc ctgccgggga atacggatca gccgacgagg
     7081 ctcccttcgg tgccgccctt tgccatgttc cgccgcatga tgacgaaaag gatgaaggac
     7141 gggatcaggg agatcgtcac gccggccagc acgagctgat agttggtgtc ctcccaggaa
     7201 ttcagcttgg tgatgcctac catgatggtg cgcatatcgg gcgcgctggc ggtgatgatc
     7261 agcggccaga gataggcgtt ccagatcgtg atgaagctct gcacgaaaag cgtcgtgatg
     7321 atcggcttgc agatgggcat caggatcgta agaatatacc gcagatcgcc gcagccgtcg
     7381 atcatggcag catagcgcag atccttcgga acggaaagga atttctgccg gagcatgaac
     7441 atctggctgg cgctgacaaa gccggtgatg cacatgccga gataggtgtt gagcagcccc
     7501 agccgggaca cggtcacata gttggtgatg aggagcgtgt cgccgggcat catcatcgtg
     7561 ccgagaatga ggaaaaagca gaagttcttc ccccggaact catagtgcgt cagcgcaaag
     7621 gccgcgcaga tggaaaacac cagacggacc actgttccga gcgtcgccat gatgagagaa
     7681 ttgagcatat agcgcagcat gggcgcctga tgcatggcgt ccctgaagtt ctgcgtatag
     7741 gcaaagctgc tcggcagcag ccgcggcgag agaaattccg ccggagtttt gaaggcgccc
     7801 agaacacagt agatcaccgg gaagatcagc accagaccca gagcgatggc cacgacggat
     7861 acgacgatgt ccgtcgtgcg ttttctggct gtatatttca ctgataaaac accttcttcc
     7921 gctcataaac aaacgacacc agcgtaaacc cgaaggtcag aacgaaagcc accatgctca
     7981 cgaccgccgc cagaccgtag ttggagctgc cgtagccgga ggagaacatc atgtacatca
     8041 gcgttgtcgt cgcgcgggac gggcctccct gcgtaataat gatcatcggg ccggatgtca
     8101 tcatcgccag gaccgtgttg gtgcacatca cataaaaaat ggtagaggag atcatgggga
     8161 gctggatctt gaatacgcgg gtgaaaaatc ccgccccatc cagctcggcc gcctccataa
     8221 tgtgcttggg gatcccccgg aacgccgatt ggaacagaag aaaatcgaag ccgatgccca
     8281 tccacaccgt aaggataatg caggtgtaca gcgccgtatc ccggctttca aaccatccca
     8341 gattcgtccc gaagaacgcg ttgacccagc ccagcgtcgg gctgaacatg gatttgaaga
     8401 tcatgcaggc cgccgacatg gatacggcca tgggcagagt gaacatggtc tcgtataccg
     8461 ggctgagctt acgctttttc gtagccagca gcgccagaga gatcgtcagc accagcgtta
     8521 ccggcacgtt gatcagcgta atgacaatgg agtttcgcag cgctttttta aagtcgttcc
     8581 gcccgaagac atatttgaaa ttctccagtc ccacaaagcg caggatctcg cccttgtcgt
     8641 tgaccgcgca aaggctgttg accagcgttt tcaaaaacgg ataatagaca aacagcagcg
     8701 caaagatcag aatcggcgcc ataaaggccg cgactttcag attcttacgg gcgttgggac
     8761 ggaggccgcc ttcgcgcagc cttccgtatg cggcgtcttt cgttattgtg ctcaatcctg
     8821 tatcctcccg ttttcccgaa acccggcggc cgccggaacg cgcgtccgcg tgctgcggcc
     8881 ttttgtctgc ttgttgcggt accttatgtg tattttgtaa catagtgttg acaaaagttc
     8941 aattcttttt tgcgtaaaat ccgcaaatca tgaaattgcc caattttttc cgaccgcgtt
     9001 ctgccgtgtt cgggaaatgt acaacgcggc attttccgcg tttcccggac aaatcggatt
     9061 gtatcgcacg cacggtaaag agagaaacac cttttttgta aggtttcggt aaaatcttct
     9121 ttaccgcctt cggttttttc accggcttgc ttgacagccc aataccgcta tgatatgatt
     9181 cggtgcgaca aatgaccaaa acaagttaca aatgtaatta cgacatatag aaagcgaggc
     9241 tgcaacgata tggcacagac cgtaagcaga atgaaccata ttttcggcac cgacgggaaa
     9301 accttcattc tcgccatgga tcacggctcc aatttcaacg tgctgcccgc catgaaggac
     9361 acgaaaacgt tgatccgcga gctggccgcg gcgggcgcgg acgccttttt gtccaccatc
     9421 ggaatggcgg aaaactttgc cggcagcttt ctgggcaagg gcatcattct tcgcatcgac
     9481 ggcggcgttt cctttctggg cgatcacaaa gcgccgatgc agatcgttgc cacggcggag
     9541 gacgcgctcc gtctgggcgc cgattccgtt atcacgatga gctttcccgg ctccgtacac
     9601 gaggcggagg ttttgagcaa tctggcgcgg gtgtgtctgg actgccacaa atggggcctg
     9661 cccgtcacgg ccgaagcgct cccccgcggt tttgaaaagg ccgaggattc ccgcacgccg
     9721 gaaaacataa cgtttgcctg ccgtcaggcc gcagagctcg gcgcggacat cgtgaagacc
     9781 gtctataccg gcgatccgga gagcttccgg acgctgacgg aaagcgttta tgccccggtc
     9841 gttattctcg gcggcgcaaa aaaggtcccc gaacgggagc ttctgcagga gatccgcgat
     9901 gcgctcgatg ccggagcctc cggcgtcgcc atggggcgga acatctgggg gaacgaagac
     9961 ccggtacgct atgccgcggc gatcgcaaag ctgatccacg aggattgcag cgtggacgcg
    10021 gcgctgaagg aaatgaaccg gctttactga aaaggggata gacggaaatg gaaacatata
    10081 aagtaagcat tcattccggc gtccgcgaaa tggaaacgcg gattctgcaa aagagagagc
    10141 ctgccggcga cgaggtgctg ctgaaggtaa cgagctgcgc catatgcacg ctcgagcagc
    10201 gcatctggaa gggcgtcatc gcccggtatc cgtatgccgg aggccacgag gcggccggga
    10261 tcgtagaggc cgtcggcccg gacgtgaaag gcgtaaagcc cggcgacaaa gcggctgtcc
    10321 ggctgctgaa ctcctgcggc gagtgctact actgccgcag cggtcacgaa aacctgtgcg
    10381 tggcgtcctt cgtggcgcat acgcattccg gcattatggg agcgggcggc tttgccgagt
    10441 acatgaccgt cagcgccaag gcggtgtata agctcgccga cgatgtggat ctggatcacg
    10501 cggcgctggc cgagccgctc gcctgctgcg ttcacagcat ccgcaacgcc aggatccagc
    10561 tgggcgacga tgtggtagtc atcggcgccg gcatcatggg cgcgctgcac atccagctgg
    10621 caaaaatgag cggggcgcgc gtcattgtca gcgagctcga tcccgtccgc atggacgttg
    10681 cgcgcaaaat gggcgcggac atcgtcatca acgccaacga agcggatccc atcgcgcagg
    10741 tcaaggagct taccgagggc cgcggcgccg acgcggtgtt ctgcaccgtg gccacgtccg
    10801 ccgtggcgaa gcaggcggtg gatatggccg gaaagctggg acgggtcgtg atgtatacct
    10861 cgttcttccc cgatcttccg atcgagatca gccccacaaa gctccattcg ggcgagcagc
    10921 tcctcaccgg ctccgtgaat ccgaatccgg tggatttcct gacggcgacg cgcctgctca
    10981 gccggaagct catcgacgtt tcctctctga tctccgaccg ggtccccatg gccgagatcg
    11041 accgcgcttt ccgggaggcc gtggagccca acacctaccg tattatcgta aagccctaaa
    11101 aaagggattg aaaaaagccg ggggccatgt aaaatggagg tatcaaaaaa ggatggtatc
    11161 ggccatgaat ctgcatcccc tgacgccgga acagaaaaac gaatataccc ggatcgccta
    11221 ttactactac gaagccggcc agacgcagga ccagatcgcg cagcggctgg ggatctcgcg
    11281 ccagcgggtg aaccggatcc tggcggagtg cattgagcgg ggcatcgttt gcatcacggt
    11341 cgaccggagc ccggaggagt atttcgcgag cgaatcggcg ctggaggaaa aataccgttt
    11401 gaaggccgtt cggctggcgc acagcctcgg ggcggaccag ctgtatggga atctgggcgt
    11461 tgtggccgga cagtatctca agtccatcgt caagcgggga gatatcatcg gctgcgttcc
    11521 cggccgcggc gtggccgggc tggtggataa catgccgcag atggagcgga ccggccttac
    11581 ggtaacgcag ctcatgggca gcgagagccg gagagagtat aatctggagg tcgacagcat
    11641 cctccaccgg tttgcgcgaa agctctccgc cctgccgcag ccgctgtatg cgcccgtgct
    11701 ggtcagcaat gccgagctgc gcgaatccat tgtccgcgag ccctattatc aggaggccta
    11761 cgccgttatg aagcggtgca ccgttgcggt cgtcggcatc ggcacagcca ccacctatga
    11821 gcagtatatc accggcgcaa accggcagcc cggcactgcg gccgccggcg cggcggctcc
    11881 ggtgggagaa atatgcaccc actgcttcga tgcggacgga cagccggtgg agtttcccct
    11941 ttccaaccgt attctggcca tctcccgcga ggattacaag aacatcccga cgcgcatcgg
    12001 cattgccggc gggccggaaa agctcgccgc gatccgcgcg gcgctgcgcg gaggctatgt
    12061 caacgtcctg ataaccgatc tggatacggc agccgccctg ctcgacgaga catgaccggc
    12121 tgcgcgctct ccccgttgta ataataagag acggatacgc ctgacattgt gtcagaggta
    12181 tccgtctctt ttcatatccg cccgcggcgg atgcggcgcc ggcgctcctt ttgccggacg
    12241 gcctcctcgc ggctgacagg tgcgccggcc gcggtcatgc gctgctgatg gcgcgcggca
    12301 tagcagatcg ccgcggccat gacgagatcg tcgtgctttc cggcgagcgc ttccgggcgg
    12361 tgatcctcgt tgtagcagaa cgtcagcatc tcgccgagaa gctcgcggtc ggaaaaccac
    12421 tccgggtgca gggagaacac ctccacaaga ttggcgatcg cgcgcgggcg cgtctgccgg
    12481 tcggtgcgga agccgcagct ctcgcgcagc cggtgcgtga acgtgtcctc ccgctcgcgg
    12541 cggtactggt tcggatagcc gagctccaga agcttcatca cgggatacgt tgagaaattc
    12601 gtttcgagcg cgacgagcgc gtcgttataa tgcctcccga gcgcgtacac ctgccggaca
    12661 tactccggct cggaaaaccg ccgccggagg cacgccgcct gttcgccggt gctgttgtcg
    12721 atgacgtgcg cggtgaacca gtccgagccc tcgcccgcgg tgtcgccgcc gagcacatac
    12781 ggatggcccg gctcgcattc gcggtaaatg cagatctcgc ccgcgtcgtc accctgccag
    12841 acgctctcgc cgtctttatc gccggggacg aaccgcccgc ggcgcacggg cggcgcggcc
    12901 tcctcgcggc ggcggatgac gagggcgtta tcgaaaacgc ccgagccgct gtggagaaac
    12961 gcctcgtccg gattcgcggg gtattcctgg cggaacatat cccggctgcc gccgcagttg
    13021 ttcgcaatgc accagcgccg ccagcgcagc tgctcccacg agagggaata ccgctcgcgc
    13081 gtctcgcgct cctccgccgt ccattcgatg cccggttcga ccggcatcgc ataatccggg
    13141 ttttcaaacc aggcgaagaa caccggctca aaatcgttct ccccggccac ggcggcgtcc
    13201 caccggtcct tgaaatcctc gcagccgttg gcggtgctct cgatgatgac catcgtcccc
    13261 ggccggctcg gcacggcctg caatatgccc gcgagcgtcg cggccttgct ctcgccgccc
    13321 tccggccaga aggcgtattc cgaaagatgc acgcactgga gcgtgtcgct ccgcccgatg
    13381 ccgcgcccgc ccgccgtggc gcagcgaaag cgcgaccgaa gccccggcag cgcgccgcgc
    13441 gatgcgcgcg acgggttttc aaacaccagc tcctgcgcgt ttgaggtgcg cagcatcggc
    13501 ttgaccggcg cgggcagctc gtcataaaaa agcttgctca tgcgaaaaag attggccgtg
    13561 gcgtcctcgc ggtgcgccac gacgagcgcg ttcacgtttg cgcgcgtggc gcacgcgtgg
    13621 aagagcagcg cctccgtgag tgtggaaaag ccgagctgac gggcctttaa aatgatgatg
    13681 cgcacgggct tgccctcgtc ctgctggcgc ttggccacgg cgtaaagctt ttgctgcgcg
    13741 gcgttcaggc gaaacggcac gaccgcgccg ctcttcgtcc gtattttgag gcacgattcg
    13801 atgtattctc tcgcaacgca cggattcaca gcgcgctcgc cccctcctgc cgcgcgagcc
    13861 attcctcaag cgagagcacg ccgtcctccg tttcctcctc gcgcagggcg cggagatgct
    13921 cgtcgagctc gtgcagcgcg cgcatcgcgc ccgcggggtc aaattcccag atgccgtccg
    13981 gctcgcgctg ctttgtttcg gggttgcgcg tgagatgggg cgtcgcctgc atgcaccggt
    14041 caacgatctc cacgagccgc cgtccgatcc acgcgctcga aacgcccatc gcgtcaaaaa
    14101 gccgctgctc gctctcgcgg cggcagcgct gtaccgctgg gtcgcgcaga agctcccggg
    14161 cacgctcttc cgctgtggcg gggctgtatc cggcggcaat ggcggcctca gggccgtcgc
    14221 cgcaggcgag atattcctcc acaaagcgct tctgccgcgc ggtgagcgcc gttttgtcct
    14281 tcattcggac cctcctttcg tgttcacagg ggcatctgct cgagaaagtt cttccgcagg
    14341 cgggagagct tccggggcga gacgcccgcc gccccggctg ccgcctcgag cgttttctgc
    14401 tgcacgagca cgccgaaaag cgcccgtccc tcctccggcg nnnnnnnnnn ctccggcgtc
    14461 tccgcaagac acgcgatcag cgcgcggacc gcctcccacc gctccggcgg aagctgcgca
    14521 aacacgcgga gcatcgccag aatgtaccgc tgctccgccg ccggacggcc gcaggacaag
    14581 ctccgatatc ccatcgatcc ataccccctt ttgtattata ggacacaaaa cgtgaataca
    14641 gcgagcaaaa aataaaaaca gaagaaagtc ggtctcctgt attacacact tcgtgtatta
    14701 acgttatacc atccgggaat gggcttgtca acacatttcg tggaattccg gttgactttt
    14761 tttcacgccg cgtgttatgg taacggtgag gtgatagcaa tgtccggttt cagcgaaaga
    14821 ctgtcggcgc tgcgcagggg cgcggggatg acgcaggcag agctggcgaa aaagctcggc
    14881 atttccaaaa gttcggtaag catgtacgaa tgcggcaatc gtgaaccgga gtttgatctg
    14941 ctggaaaaaa tggcggatct cctccaggtg gacatgaacg ttctgcttgg ccgcggcgag
    15001 ccgctcgtga acaacgatcc ggagctgacg gcgtatctgg aagcgctgcg cgacaggccg
    15061 gagatgcgcg cgctgttccg cctttcccaa aatgccacgc gcgaggatgt ggagactgcc
    15121 gtgacgatcc tcgaagcact gcgcagcaag catgaggata cctgactatt acgtccggtt
    15181 cgtggacttt ccgccgacgg tgctcggcgc gacgctcccg aacgacgacg gcacgttttc
    15241 cgtatacatc aacgcccgtc tcggcgacga cgcgcggcgc gaaacgctgc ggcacgagct
    15301 cgagcacatg gcgcgggacc acttttaccg cgacgctccc atcgccgcgc aggaggcgga
    15361 ggcccgcggc gagacggtct cggcagtacc gccggcgccg gagcgcggca cggtgcgctg
    15421 ttaccgcgat ctcgcggcgc tcacggcgta tctccgaagt ctcggcgcgc tcggcagcag
    15481 catggaatcc ctcggcgccg agccgatgcg gtgataaaaa agagccaatg gcatttgtgt
    15541 gcctttggct cttttgccgt agtttgtcag ttcagcggga gaattttgca ctcggagccg
    15601 gagagctcct gcgcgggggc ggggagcgtg tccacattga tgagcggccg gccgccggaa
    15661 acgagaatgt ccacatactg ctctgcggag agctcgcgca cgacgatctc gccgccggag
    15721 cgccggtaca gcagcgccgg aacgccgccg tcggtcgtga ggacaacgcc gacatcgtag
    15781 gcatgctcgg ggtcgagccc gtccagcgcc ttggcgccgc ggcgctctac gtttttgagc
    15841 accggcatgt ccgcctgact cacttccggc gcaccgctgc cgctcgtgag cagcacgacg
    15901 cgcgcgtccg tcgccgtggc cgagagctcg gtcgaggcga aggggtcaaa ctgccggttt
    15961 gtggagatca cataaaaccc gttctgctcg atgcgcagcg cgcggctttt atagagctcg
    16021 cgcacggagg tcaggagctt tacgtatttt ccttcctctg cggaaagaac ggcttcaata
    16081 tcctcgcgga tgcgctcact gcggcgcgcg aagaaggtgg agaacatggc gctgtcgccg
    16141 tcgtcggtct gcaatgcgag cagccacgcg agaagctccg cgcggcttcc ggcgggcagc
    16201 tcgtcctccg aggggacgtc tcccgcggcg agccggtcga gaagcgctgc ggcgctctcg
    16261 gttttatcgt ccgcgccgaa gaagcggatc ttttcgagaa gctgcgcatc ggggtgaagg
    16321 ctgtcgcccg ccggaagacg cagcggcacg gtctccacca cggcggcact gccgtcaaga
    16381 tatacgtccg cgacttcctg cgtgcgcccg gtcgcggtaa ggtccacgcg gaagtcctcg
    16441 cgcacgctgt ccggccagag cgatttcggc agcgccgtcc ggctgccgag aataacgctc
    16501 tcggcatgg
//
LOCUS       DAWAAE010000028        16153 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1629_length_16153_cov_2.857622, whole genome shotgun sequence.
ACCESSION   DAWAAE010000028 DAWAAE010000000
VERSION     DAWAAE010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16153)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1629_length_16153_cov_2.857622"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1055..1064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2562..2571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3591 a         4578 c         4857 g         3107 t
ORIGIN      
        1 gcatcgattc ctgcggtatg ctcatctccg ccgtgcatca cgaaaacggc gaggagcgcc
       61 tgcacctcct gatggtcgat cctcacatcc ccgccggcgc aaagctctat tgattgtccc
      121 aaaattggcg gcgcttacgc cacagcttac gccaatttac tccgagctta cgccagaaat
      181 gcgtaaagaa acgttcctgt gatttaagcc cgctgatttc ggtcagcggg cttttcagta
      241 ttttttcagc ggatgaaatt gacaaatttt tgtacatctg ttaaaataag taaacactac
      301 accaagtatt tggataatcc tggcgaacaa ttcttcggaa aggcggagcg aaccgattgg
      361 aagataaacg tatcaccaaa acgaaacgca atatcaaagc gacgctgaca gcccttttgc
      421 aggaaattcc atttgagaaa atctccgttg cagagacctg ccgccgcggg gatatcagcc
      481 ggatcacctt ttacgcgcac tatgaggata agtatgctct cgccgaggag atattttccg
      541 atcatgtgca ggaagcccaa gacaaatacc acatgctgca ggcggagaat aatccgaaaa
      601 acaacggctt catcggctat cagaatctta tggatgccat tttgagcctg tattacgata
      661 actacacctt cttttcccat acaacctcaa gcgaaaatcc ctatcttttt tcttcgtact
      721 ttaaccggat gaatgccagc gtggatgact atctccgccg tcatacctac ctgaaaccgc
      781 gtttcccgtt ccaccagacg gcggcgctca tatgcaacgg cctgttcggc gttatcaaca
      841 gctgcgtagc ggatcacctc tcggaggcgg aaacgcgcgc tctggtccgc gatatatacg
      901 ccgttctttt gagctcggat atgtttgaaa aataagtggg gaaaggaata ctttcatggc
      961 gctgttaacg caaaaggcga tcctctcgac ctttgaagag atgctggagg aaatgccgtt
     1021 tgacaagatc accgtatcgg ctctcgtccg gcggnnnnnn nnnntcacga atcttctctc
     1081 ctccacctcc cgcaggcatc tggagcagta tatttttacg gaatcggaca ttttctttta
     1141 cgattatgtt cgggaacgaa ccgccggtat gccgctttcg gaggaattga tccggtacat
     1201 tgcgcagttc taccgttact cttttatcgg cttcttcctg aaatttacct gggagaagat
     1261 gcagaccgat gtggatgaaa gcgtggaccg gctcgagaaa gtattcaacg cctttattga
     1321 cagcgctgcg gcgcaaatgg aaagagaccc gctgtaaaaa agcagccggt ctcttttatg
     1381 cttttatgtg aaaaagtttt tgcgcataaa cgcgaaaata tcataaatgg ggcacttttc
     1441 cagatgtgtt agaaaatacg acaacggcgt cggtttgtcc atggaaaagc cggtacgatt
     1501 cgatataatg taagaaaaat cctataaagg agcgtgtttt taatgagcgt atcgacgacc
     1561 gtgaagaagt acggcatcga acaggcgatc aaattcatgt acaaggaccc cgagaagaat
     1621 ctgcccaagc tgatggactg ggcggacacg ttctgcaagg gacgcttccc gggccagcgc
     1681 gccgcgatcc gccgcgccat cgaggatccg acgtatccgt actatgactt cgtccgccac
     1741 atgatgaatg aagtcgatca cgatgttctc acgacgatcg tggtgaattt cttcatcaac
     1801 gccaacatgg tcggctgcga aaagcaggaa aagctccgcg aggagcataa ctgcaacatc
     1861 ccctgggcga tccttcttga tcccaccagc gcctgcaatc tccactgtac cggctgctgg
     1921 gctgcggaat acggcaacaa gctcaatctc acctttgacg agatcgacga catcatccgt
     1981 cagggcaaag acatgggcgt ttacatgtac atctacaccg gcggcgaacc tctcgtccgc
     2041 aaggcggatc tcatcaagct ctgtgaaaag cacgacgact gcatcttcct cgcctttacg
     2101 aacggcacgc tcatcgacga aaagttcgcc gacgatatgc tgcgcgtgaa gaactttgtc
     2161 ccggccatct ctctcgaggg ttttgaggaa gcgaccgatt cccgccgcgg tgacggcgtt
     2221 tacggcaagg cgacgcacgc gatgaacctt ctccgcgagc gcaagctgct ctacggcatt
     2281 tcttcgtgct atacgagagc gaactttgag tccatcacct cggaggagta ctatgacagc
     2341 ctcatccgga tgggcgcgta cttcatttgg tacttccact acatgcccgt cggcaacgac
     2401 gcttcgcccg agcttctccc gacgccggag cagcgcaccg aggtgtaccg ccgcatccgc
     2461 cgctaccgcg ccgagaagcc gctcttcgcg atggacttcc agaatgacgc cgagttcgtc
     2521 ggcggctgca tcgccggcgg cagcacctat ctgcacatca annnnnnnnn nagaggatga
     2581 aatccccgct cttcatggcc taccacgacg gacaaccgtt caacgacaac atgctccggc
     2641 cctgcccgat gctcgaaaac ccggagaagc tgcgcgctat ggtaaagtcc tccggcgcac
     2701 attccacgga tttgcagtcc ccggagactg tcgatcacct ctgcgccaag tgcgaccggt
     2761 atgccgccga gtggaagccc acagcggata agctctgggc ggaaaaccgt gcggagcatg
     2821 acgcaaagta aatcggaaac cctgtatacg gatgcggtaa actgccgcat ccgtgtacgt
     2881 atatttatga ttcccaagaa aggtgaatgg tcatggaaaa gaaatctctg aaaacctata
     2941 aaacatgggc gatcgtactc gccgttgtta ccatggtact cggtctcatc atggtattct
     3001 ggccgggcat ttcggcggtc gcgatctgct atctgatggg cgcggtctgc atcgccgccg
     3061 gtatctatga gatcatccgg tattttgatc tcggtcttat cggcatactg ttccggtacg
     3121 acctgtttct cggcattatg agcgtgcttg ccggcgtgct gctgcttctg cacccgaacg
     3181 gagcgcttgt cattttgccg gtcattctcg gcttttatat cattcttgcg agtgtgttct
     3241 ctatccagat ctcgacggaa gcccgtcatt tcggcttctc cggctggtgg ggcgcgctgc
     3301 tgctcggcat cgtgggcgcg gtactcggcg tgctgatgat ctttgatccc tttaagggcg
     3361 caaaggcgct gatgatcttc accggcattt cgctgcttgt gacgggcgtt gagagcatct
     3421 acgctatcgt ctgcatttcc aaggtgttcc gctcttccgg acaccagcag atcatcgatg
     3481 ccacgtggca cgagatttga ttcatacgat atagcaaaag actccggctg aaaaccaacc
     3541 ggagtctttt tgtccttttc cgcattcacg ccgaaaaacg ggaaaaaggt tgaaaagagg
     3601 gaaccgcggg ggtaagataa aatcataata tagagcagga ggccgaaatt gaaattcttt
     3661 catttgtccg atctccatct cgggagacgt atctatgagt tttccctgat cgatgaccag
     3721 aaatacattc tcaaagagat cctccgccgg acggacgagg agcaccccga cgcggtcgtg
     3781 atcgcgggcg atatttacga ccgcgccgtg ccgagcgccg aggcggtcga gcttttcgat
     3841 tcttttctta ccgagctggc cgggcggcac attccggtgc tcgccattgc cggcaaccac
     3901 gattcgccgg agcgcatcgc gttcggcgcg gggctaatgt cgccgagcgg cgtgtatctc
     3961 tcgccggtct ataatgggcg cgtggagccg gtgacactct cggacgccta tggcgaggtg
     4021 cggttttatc tgctcccgtt catcaagccc gcgaacgtcc gccgctttta tccggacgca
     4081 gtcatcgaga gctataccga cgcgctccgc tgcgccgcgg agcatatgaa tattgagccg
     4141 tcgacgcgga acgtgctcgt aacgcatcag ttcgtcaccg gcggcgtgcg ctcggagtca
     4201 gaggatatca cggtaggcgg cacggacaac gtggatgccg cggtgttcga cggctttgac
     4261 tatgttgcgc tcggccatct ccacggcgcg cagagcatcg ggcgcgaaac gctccgctat
     4321 tccggtacgc cgctcaaata ctctttctcg gaaaaggatc aggagaagag cataacggtc
     4381 gtggagctga gagagaaggg aagcgtatcg atctcgacgc tgccgctcac accgcagcgc
     4441 gatatgcgcg aagtgcgcgg cacctatgac gagctgacgc tccgcggaaa ttacgccgga
     4501 acgaataccg acgattacat ccacgccgtt ctcacggacg aaaacgacat tccgaacgcg
     4561 ttttcgcggc tgcaaagcat ttatccgcag ttgatgaagc tcgattacga taaccggcgc
     4621 acgcgaacct ccgttgtgat cgctgcggaa gaacgtcgcc ggacgatgac cgatacggag
     4681 ctttttgccg agttctttga aaagctgaac ggacagaaga tgagtgccga gcagagcggc
     4741 tatgtaaagg ccgtgtttgc cgcgcttcgg gaggaaatgc tatgagaccg ctgaatctta
     4801 cgctcagcgc cttcggcgcg tatgcgggca aaacggagct tgatctccgg cagctcggcg
     4861 aacgcgggct gtatctcatc acgggcgata ccggcgcggg gaaaacgacg atctttgacg
     4921 cgatcgcttt tgcgctgttc ggtaaaccga gcggcggcaa gagcgggcgc gatgtgtcca
     4981 tgctccgctc aaaatatgcc tcgccggaaa cgccgacgga agtagagctc accttctcca
     5041 acgggggaaa gacctataag atcaagcgca atccgacgta tgagcgcacc aagctccgcg
     5101 gcaccggaac gaccgagaaa aaggcggacg cggaactcct gatgccggat ggcaagatca
     5161 tcacgggtac gaaagaggtg gatgagaaga tccgcgatat ccttggagtg gataaggatc
     5221 agttctgtca gatcgccatg atagcgcagg gtgaattccg cgagctgctg ctggcggata
     5281 cggagacccg ccggaagata ttccagaaga tattcaacac taatctgtat tcactcttcc
     5341 agaaggaggt aaaagaagac ttttttgcga tcggccgcga gctgaaggag gcggaaaact
     5401 cctttaaaca gtacgccgca gggatcgtcc tgccggagga caccgagctc ccgccggaaa
     5461 atgagttctc tgaatttctc gccggtttgc tgaaagccga ccgcgcgcag gatgacgcat
     5521 gggagaaaga gctcgcggag atcgggaaaa agctcgatac gctgaccgcc gatgcggcca
     5581 aagcggcggt ggacgaagaa aaccgcagcg aacttcggaa agcaaagctc tccctcgctg
     5641 aagccgaaaa ggcggtgagt gagtccggca tcgcgctgga aaaggaaaag gcgtgcgagc
     5701 cggagctcgt gaagctcgcg gaagcgctca aggcgctgga agatgagcgg aaggcgcacg
     5761 acgagctgat ggacacggaa aaaaaggccg ccgcggcgaa gaaaacggca gacgatgcgg
     5821 cggcacgcct ttcccggctc gaagaatcgc gcggtgagct gcgggagcac ctttcgcggg
     5881 agcgggagga atatcatgcg cttgccgcct ctgcggagaa tctcgcgaag ctccaacagg
     5941 aaaagaccgt tctctcccaa tatctcgacg acctccggaa gtatgaaacg gcccttcaga
     6001 cgcttgcaaa gcgcaaagat gcggcggacg ccgcgcgcga tgcctatacg agggcgcgga
     6061 gcgaagaaga gcatctttcc gccgaggcgc aggagctgcg gcgaagattc aacgacgagc
     6121 aggcgggggt gctggcctcc acgcttcacg agggcgaacc ctgccccgtc tgcggcgcgc
     6181 tgacgcatcc gcaccccgcg cagtgtacgc cggacgcgcc cgacgagaag aaagtgaagg
     6241 acgccgagaa aaaggccaaa gcggtgtggg agcgagtaaa taaggcgagc gaagcttccg
     6301 gcattgcgaa aaacgcctat gagaccgcgc agaaagagga agcggaacag agagaaatgc
     6361 tcttcggcgg gaaagagaag atcgatctcg aagccgaaaa gagggctgct gtgcgccgct
     6421 gcgccgaaaa agacggcgag atccggaaag aatcggaaaa cgccgcgcgt ttcgagactc
     6481 tcggacggaa aattccggag gaggaaaagc gtcttgccga aatggacgcg ccgatcaccg
     6541 aggcgcagaa gacggctgcc gacacgcaga gcgcgctcac gtctctccgt cagacggcgg
     6601 acaagcttcg cgcggggctg aaatacgaaa atgcggcgga ggtcgaaaag gaaaagcggc
     6661 aagtccttgc ccgtcagagc gagatggaag atgcgcggaa aagagccgag gaagcgctcg
     6721 tcgctgccaa ggaacggcgc tccgcagagc agggaaaggt ggagaagctc gaagagcttg
     6781 tttccgatgc gcctctcgtt gatcttgcgg caattgccga agagaaaaat tgtctcacac
     6841 agcggaagga cgacctgaca aaccgccgcc gcgctcttga ccggcgcatc acggcaaacg
     6901 acagcgccaa aaagggaatc gacaaaaaag ccggcgagcg ccgcgcgctc gaggagcgct
     6961 ggacatggat gaagccgctc tcggaaacgg cgaacggcgg tctcaaggat catatctcgc
     7021 tggaaacgta tgtgcagatg acgtattttg accgcaccct tgcgcgcgcc aatacgcacc
     7081 tgatgcagat gtccggcggg aagtacgatc tcaagcggcg cgagggcgca gcgaagaatg
     7141 gcggccaaaa cggtcttgac cttgacgtca tcgaccacta caacggcagc gagcgcagcg
     7201 taaaatcact ctccggcggc gaaacgtttc tcgcctcact ctcgctcgca ctcgggcttt
     7261 cggaggagat ccagtcgagc gccggcggcg tgcagatgga cacgctcttc gtagatgagg
     7321 gcttcggctc gctcgacgag gaaacgctcc ggcaggccat gcacgcgctg cgtgagctct
     7381 ccgagggcga ccggctcatc ggcatcatat cccacgtttc cgagctccgc cgcgagatcg
     7441 accggcagat cgtcgtcaaa cgctcgaagg acggcgcaag ccgcgcggag atcataaccg
     7501 aataaaacaa aggagaaaac gatccatgaa agttctcatc atcaacggca gccctcgtgc
     7561 aaacggcaat acggacatcg cgctgcgcga aatggaaaag gtcttcgccg aagagggtgt
     7621 ggagacgaag cggctgaaca tcggcacgca gatgatccac ggctgcgttg cctgcaatgc
     7681 atgccaccgc acaggaaaat gcggctttga cgatgtggtg aaccgcgccg ccgatatcct
     7741 ccgcgactgc gacgggctcg ttgtcggcac gccggtgtac tacgcctccg cgaacggcac
     7801 ggtcgtgtcg tttttggacc ggcttttcta ctctgcaccg tacgatctca cgatgaaggt
     7861 cggcgcctcg gtcgccgtgg cgcgccgcgg cgggacgagc gcaacgtttg acgagctcaa
     7921 caaatacttc accatcagcg gcatgccggt cgtatcgagc cagtactgga acagcgtcca
     7981 cggctgccag ccgggcgagg cggcgcagga cgccgaaggt ctccagacga tgcgaactct
     8041 tgcgcggaac atgacgttcc tgatgaaaag catcgctctg ggaaaggcaa aatacggtct
     8101 tccggagaag gaagagcgcg ttggaacgaa ttttattcgt taactctttg gaaatatata
     8161 aaatattgta ttgatttttc tcctgcgtga cgatatgata aaatgaaaac cggctccacc
     8221 ggccggcgaa gaggaaagga caggcgtttc gcctttttct atggaaacga tcacatcccg
     8281 gaaaaattcc cgcgtgcagg cgctgcgcgc gctcggcaga aacaaggcat atcgccgcga
     8341 gcagggactt ttcctctgcg acggagagaa gctgctctcc gaagcgctcg caaacggcgc
     8401 ggatatcgcg gagatatacc tgcgcggcgc aaagccggca gggaatatgc cggaggttcc
     8461 ggtgtattcg ctctctgagg atgtgttcga ctatgcctct ccgctcgagc acagccccgg
     8521 cccgcttttc accgttcgcg caaagccgct tccggagagc gtccggcccg agcgggtcat
     8581 cgttttggaa aacgtgcagg accccggcaa cgtgggtaca gtgcttcgca ccgcggcggc
     8641 gctcggcacg gagctcgtgg tgctcctcgg cgattgcgcc gatccctaca acccgaaaac
     8701 cgtccgcgcc gccatggggg cgcttttccg ccagcggctc tgggagaccg atctttccac
     8761 gctctgcgcg aagctccgcg aatgggagct tccgctctgc ggcgcggcgc tccgggcaga
     8821 gagcgttgat gtaaaatgcg tttcgctccg ccgcgcggcc atcgccgtcg gcaatgaggg
     8881 acgggggctc accgacgcgc ttctggaaaa gtgtgatacg aagatcattc tccccatgac
     8941 gcccggcagc gagtcgctca acgcggcggt cgcggcctcc atccttatgt gggagatgtg
     9001 gacacagcgc ggataatctg aggaagggag aagggaaatg tcgcagcttc ggtactgggt
     9061 atggcttgcc tccgtggacg gtgtgcgcac gatctcgctg tgccgagttc tggagcactt
     9121 cggcggggcg atggaagcct attttgcgcc cgaagagcgg ctttcggagc tcgaggatct
     9181 ttcgccgcag gagcggcgcg ccctctctga ccggagcctt gagcgcaccg aacagatcct
     9241 tgctctctgc caggagaaga atatccgcat tctcacgctg cacgatgccg cttatcccga
     9301 ccgtctgcgg cagatcgccg atccgccgct cgtattatac gtccgcggga atcttccgtt
     9361 tctcgatgag ctgcctacga tcgcgatcgt gggcacgcga aaggcgagcg cctacgggat
     9421 caatgtggcg acccgccttg ggcaggagat tacttcttac ggcggctgcg ttgttaccgg
     9481 catggcgctc ggcgtggacg gcgccgccgc ccggggcgcg ctgctcgcgg gcaagccgtg
     9541 cgtcggtgtg ctgggtacgg cgatcgacgt caattatcct gcggccaatt cgctgcttat
     9601 cgacgatgta gcatctgccg gggctgtcgt cagcgagttt cctccgctct atccgacgaa
     9661 gccggaaaac tttccccgcc gcaatcgcat tatcagcggc ctttcctgcg gcgcgtgcat
     9721 cgtggaagcg ccggtgcgca gcggcgcgct catcaccgcc aatcttgcgc tggagcaggg
     9781 gcgcgatgtg ttcgccgttc cgggtaatat cgacagcccg aacagcaccg ggactaacgc
     9841 gctcatccgc gacggcgcca aggcgatcac cggctcacag gatatccttt cggagtatga
     9901 agggctctat cccggccgca tccaatacga tacggagctg tattccccgc cgaaaagagc
     9961 ggaaaaggta tcggtttcgg caaaaacaga gattgacaag ccgcagacga taccgtatag
    10021 tgacctcgaa agccgtctga cggagtacac ggaggaccac cgggcacttc tgcgctgcat
    10081 ttcgcggggc gagacccatg tggacgaaat ggcggcttcg accggctttt ccgcggcaaa
    10141 ggtgctcgcg tgcatgacgg tgctcaccat ccgcggcgca gtgaaacccc tgccgggaaa
    10201 gcgatattct ttgaacctga agtaaggacg atagtatatt atggcgcaag caaaaaagga
    10261 tcttgtcatt gtggagtctc ccgccaaggc acggacgatc gaaaagtacc tcggcggcga
    10321 ctataaagtt attgcatcca tgggccacct ccgcgacttg ccgaagagca agctcggcgt
    10381 ggacattgaa cacggcttta cgccggagta tattccggtc gctgcgcggt cggacgttat
    10441 caacgagctg aaaaagcgtt ccaaagaggc cggaacggtg tatctcgcca ccgacccgga
    10501 ccgcgagggc gaagcgatca gctggcatct caaggagctt ctgaagatcc ccgacgagaa
    10561 ggcccgccgt gttaccttta acgagatcac gaaaaaggtc gtgcaggaga gcattgccaa
    10621 cccgcgcgat atcgatcagg atctggtgga cgcgcagcag gcgcgccgca ttctggaccg
    10681 tatcgtcggc tataagctca gcccgctcct ctggcgcaag gtgcgccggg gtctctccgc
    10741 gggccgcgtg cagtctgttg ccatccgcat ggtcgtggac cgggaaaacg agatccgggc
    10801 tttccagccg gaggaatact ggtatctgga tgcacatctt gaccgcgccg ggaagagggg
    10861 cggctttgtc gcccgctatt acggcacggc agacaagaaa aaagagcttc acagtgaaaa
    10921 agaagtggag gacgtcatcg ccgccgtgga aaaggcggcg tgggagatcg gctccgtcaa
    10981 gcgcggcgag cgccagcgca gcccgtcgcc cccgttcatc acctccacgc tgcagcagga
    11041 ggcgagccgc cgtctggggc tcacgccgcg gcgcacgatg agcattgcac agcagctcta
    11101 cgaaggcgtg gatatcaccg gcgaaggcac ggtcggcctt atcacgtata tgcgtaccga
    11161 ctctctgcgc ctttccgagg aagccatggt cgccgcgggc agctttatcc gcagctttta
    11221 cggcgaggag tactattacg gcaaaccccg ccgctataag accaaggcgg gcgcgcagga
    11281 cgcgcacgaa gccatccgcc cgagcaacgt gaatctcacg ccggagcgcg tgaaaaagga
    11341 tctcacggcg gagcagtacc gcctgtaccg tctgatctgg ggccgcttta cggcgagcca
    11401 gatggcaaac gccgtctatg ataacgtggt gatcgatgct ctcagcgccg gacactgctt
    11461 caaggcgaat cattcctcgc ttcgtttccc ggggtatacc gcggtatacg aggacccgaa
    11521 cgaggacgag gagaccggcg ggaaaaagcc gctgcccgat cttgccgagg gcgagatcgt
    11581 aacgctcaaa aagctcgaca aggagcagcg tttcaccgag cctccggcgc gttatacgga
    11641 agcgacgctc atccgtgcca tggaggaaaa gggcatcggc cgtccgagca catatgcccc
    11701 gaccatcagc accatcaccg ggcgcgagta tgttgccaag gaagggaagt acctcaagcc
    11761 gacgtccctc ggcgaagtcg tcacgggact catggaggag cgtttccccg atatcgttga
    11821 tctcaaattc accgcgcata tggaagaccg gctcgatgag atcgaaaacg gcaagatcga
    11881 ctggaaaaac gttctggaag atttctacgg cgattttgat aaggaactga ccgaagcgga
    11941 aaaggcgctc gagggcgtgc atatcaaagt gcccgacgag gtctccgacg aggtgtgcga
    12001 caagtgcggc cgtcatcttg tcgtaaagag cggacggttt ggccgcttcc ttgcctgccc
    12061 cggcttcccg gagtgcaact ttacaaaacc catcgtcatc gaaatgcccg gccggtgccc
    12121 gaagtgcggc ggacgtatct tcaagcgcac atccaagaag ggctatacgt attacgcctg
    12181 cgagcgcggc gcggagtgcg gctttatgac gtgggacgtt ccggtcaagg acgtttgccc
    12241 gtcctgcggc aaaacgctct tcaagctctc gggcaagggc gcgagaaagc cgttctgtgt
    12301 caatcccgag tgcgacatgt tcgtgccgga ggaaaagcgc ggctaccgcc gcaaggccgc
    12361 cgcggataaa acggccgaaa agccgcagga atcggcggca gagagcacag aaaagaaaga
    12421 cacaccgaaa gaggataaat gaacgctgta acggttgtcg gagccggtct tgccggttcc
    12481 gaagcggctt atcagctcgc catccggggc atcccggtgc ggctgatcga aatgaagccg
    12541 aaaaaaatga ctccggcgca cgtctctccg tattttgcgg agctcgtgtg ctccaactcg
    12601 ctgcgcagcg acgagcttac caacgccgtg gggcttctca aagaagagct gcggcggctc
    12661 ggcagcatcg tgatgcgctg tgccgatgag caccgcgtag ctgccggcgg cgcgctggcg
    12721 gtggaccgcg aaggctttgc ccgcgccgtg accgaggccg tccgcgccct tcccaatgtg
    12781 aagatcgttg aggaagaggc ggtggatatt cccgacggag aagtcatcat cgcaagcggc
    12841 ccgctcacct cggacgtgct cgcggaaaac atccttcgcc gcaccggcag cgaagggctg
    12901 cacttctacg atgccgtcgc gccgatcgta tcgctggatt ccgtcgatat ggaaagcgcg
    12961 ttccgcgcct cccggtatga taagggaact gcggattatg taaactgccc aatgaccgag
    13021 gaggaatata ccgcgttctg gaaggagctg acgactgccc gcgaagcggc ggttcacggc
    13081 tttgacgacg gcggcgtatt tgaaggttgc atgccggtcg aggtcatggc gcgccgcggc
    13141 tttgatacac tgcgctacgg gccgatgaag ccggtaggac tgcgcgatcc gcacacgggg
    13201 cacgagccct atgccgtggt gcagctccgg caggacaatg ccgacggtac gctctataac
    13261 ctcgtcggtt tccagacgca tctggcgttt ccggagcagc ggcgggtttt ctcgatgatc
    13321 ccggcgctgc gcgaggcgga gtttgtccgc tacggcgtga tgcaccggaa tacctatttg
    13381 cagtcgcccg gaaagctcga ccggtattac cggctgatcg ctgagccgcg tattgccttc
    13441 gccggacaga tcaccggtgt cgaggggtat gtggaatcct gcgcctccgg catgctcgct
    13501 tcgcttgaaa tggcggcgcg gcttttgggg ctgccgccgg tcgatttctc gcaggaaacg
    13561 gccatcggcg cgctggggct gtatatttcc cacggcagca tcggcgattt ccagccgatg
    13621 aacatcaatt tcggcatcat ttccccgctg acctaccggg tcaaggggaa aagaaataaa
    13681 aatgcggaga tctcggcacg ttcgctggcg cagatcgacg agctggtgaa aggagacctt
    13741 tggaatgcgt attctcattg acgccatggg cggcgataat gcgcccggcg agatcgtccg
    13801 cggcgcggtg gaagcttcgc gcgagtttca tgtggatgtg gcgcttgtgg gccgagaggc
    13861 ggagatcctc gcttgtctga acgagatgga tgtcatgccc ggcgaacata ttcagatcgt
    13921 gaacgcgccg gacgttattt ccatggagga tgacccgagc acagccacgc gcaaaaagcc
    13981 ggagtcttcg atggccgttg cgctccagat gcttcaccgc ggcgagggtg acgccgttat
    14041 ttccgccgga agcaccggcg ctcttttgag cggagctacg ctcacggtgc gccgcatccg
    14101 cggcatccgc cgtgcctgct ttggcccggt gatcccgaac ggcggcaaag gtacgcttct
    14161 gatcgactgc ggcgcgaatg tggaatgcac ggcggagtat ctgctgcagt ttgcctatat
    14221 gggcaagttc tacacgcacg atcttctcgg gtgcgaagat ccccgcatcg gtcttttgaa
    14281 tatcggcgcg gaggagacga agggaacgga gcttcagaag cagacctatg ctcttctgaa
    14341 ggaagctcac gaggctgggc ggcttaactt tgtcggcaac gtggaagcgc gcgacgtttt
    14401 ctccggaaat gtggatgtcc ttgtgtgtga cggtttctcc ggaaacgtgc tgctcaagtg
    14461 cattgagggc acagccatga tgcttatgaa acgtctcaaa ggcatttttt ataaaaacac
    14521 cgccaataag ctcgcggctg ccgtgctcaa aaaggatgtg tacgcgctca agtcgctctt
    14581 tgatccgagc gaggtcggcg gcacggcgct gctcggcatt tccaagcccg tcattaaggc
    14641 gcacggctct tcggatgctc gcgctatccg cagcgccgtc cggcaggcaa tcacattcat
    14701 cgattctgac gtgatcggca gcatcgagaa gaatgttgca tatatgcgta tttcaccgaa
    14761 cgaaggagaa taaaaagaca tggtactcga acaaatcatc aaaatggtcg ccgagcagtt
    14821 tatgatcgac gaaaacgaaa tcagcgcgga tacgtccttt gtggacgatc tcggcgcgga
    14881 ctctctcgac gtcgtggagc ttacgatggc gctcgaagag accttctcgc tgcccgacac
    14941 gccggaagac gagctgacca acatccacac cgtcggcgac cttgccgatt acgtgtcccg
    15001 cgcaacgcag gaataagcgc atgaccgctc tggaagagag actgggccat tcgttccgaa
    15061 accgggcgct gctcgaaacg gcggtaacgc acagttccta tgccaacgag aaccgtgctt
    15121 ccggtatcgt ctgcaacgaa cggctggagt ttttgggtga ttccgttctc ggcgttacgg
    15181 tcgcggattt cctgtaccgg cattttcccg atatgccgga gggccgcatg acgcggctcc
    15241 gcgcggagct cgtctgcgag cagtcgctcc atcgtgtggc gctggaactg catctgggcg
    15301 actatctccg tctcggcaag ggtgaggagc acaacggcgg gcgggagcgc gcgtcgatcc
    15361 tttccgacgc tgtggaggcg gtcattgccg ctatgtatct cgacgccggc atggaaacag
    15421 cggcgggatt tatccatcgc tgcctgctgg acgatgtccg caccatcgaa acgccgtcct
    15481 tcaccgacta taaaaccgcc ctgcaggagc ttgtgcagcg tcaaagcggg caggtgctct
    15541 cctacgagct tgtcggcgag gaggggccgg atcacgcaaa gacgttccgc atgcaggtct
    15601 gtctgaacgg cgagccggtc ggactcggaa ccggacgcac caaaaaggaa gcggagcaga
    15661 cggcggccgc cagcgcgctg gaggcgctgc ggaagtgacg ccgcgccgcc gtattcttcc
    15721 catatttgtc cctcatgcag gctgcccgaa tgactgcgtt ttctgcaacc agaaacgcat
    15781 ctccggcagc ctgcttccgg cttctgcgga aacggtgcgt gccgccgttt ccgtgctcga
    15841 gccgggcagc ggatatgagc tggcgtttta cggcggcagc tttaccgcca tacccgaagc
    15901 cgagcaggaa gcgctccttg ccgcggcgat cccggcgcgg gaatccggcg cggtttcagc
    15961 gcttcgcgta tcgacaaggc cggacgccgt gacggaggaa aagctggcgc gcctgcgccg
    16021 gtacggtgtc gagacggtgg agctcggcgc gcagtccatg tgcgatgagg tgcttttacg
    16081 cagcgggcgc gggcatatga gcgaggatac cgtgaaggcg gcaaagctcg tgaaggacgc
    16141 cggtttcacg ctt
//
LOCUS       DAWAAE010000029        15762 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1680_length_15762_cov_3.275228, whole genome shotgun sequence.
ACCESSION   DAWAAE010000029 DAWAAE010000000
VERSION     DAWAAE010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1680_length_15762_cov_3.275228"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5395..5404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13439..13448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2909 a         4991 c         4865 g         2977 t
ORIGIN      
        1 ggaaatgacg ttctccgtca acaactctcc cttcgccgga cgcgagggca agtttgtcac
       61 gagccgccag atccgcgacc gcctttaccg cgaaacgctc aaggacgttt ccctgcgcgt
      121 cacggacgtc gagggcagca ccgacagctt caatgttgcc ggacgcggcg aaatgtcgct
      181 ctcaatcctc atcgagacga tgcgccgcga gggctatgag ttccaggtct cgcccccgcg
      241 cgtgctcatg cagcgagaag ggcaatctca tggagccgat ggaagagctc gtggcggacg
      301 tgccgcagga gtatgtcggc tcggtcatcg aaaagctcgg cacgcgcaag gccgagctca
      361 aggaaatgac ccctgtgggc gcgcgcatgc gtctcatctt ccttgtgccg agccgcggtc
      421 tcttcggcta tcggaacgaa tttctcaccg acacccacgg cgagggcatt atggcctccg
      481 tgtttgacag ctacgcaccc tataagggcg aggtcacgcg ccgctcgacc ggctctctca
      541 tcgcgtggga gcagggcgag gccgtcgctt acggcatctg gaacgcgcag gagcgcggca
      601 tcatgttcat aacgcccggc actccggtct acggcggcat gatcgtcggc gtcagcccca
      661 agcaggagga catcccggtc aacgtctgcc gcacgaagca tctcacgaac acccgcgcct
      721 ccggctcgga cgaggcgctg cggctcgtcc cgccgcgcca gatgtcgctc gagcagtgca
      781 tggagttcct cgcggacgac gagcttctgg aagtcacccc caagaacctc cgtctgcgca
      841 agcgcattct tgaccacagc cagcgtatga agaacatcca cggcaaaaag taaaataaac
      901 gatcgaagcg gtctcaccca gagcagaaat gcgccggggg agaccgcttt ctatttagcg
      961 tttccactaa gaacacgccg gggaaaaagt gcaaaatgcc aaatcttccc tgcggtgtac
     1021 agttctatat tgacggatta tacaatctat actatgatga acttaccaac tggtatatac
     1081 cagttgccta ttatggcggg ctctcgccgg aacggaggac cgcgcatcat gcaggaacgt
     1141 tcgctttata aaacgatacg cgacgatctc ctccgcgcca ttgaaaacgg cagtctccag
     1201 cccgatcagg cgctcccctc ggagcgcgag ctctgccagc ggtacggcac gagccgcatg
     1261 accgtgcgtc acgccgtcac ggagctggaa tcgctcggct cggtgtaccg gatgcaggga
     1321 aaggggacgt tcgtttccgg ccgcaagctc atccagccgc ttatgcaggt gtccggcttt
     1381 acggaggata tggcaaaacg cggcaagaag gcaagctcgc agatcctctt cgccggggag
     1441 aagaaggcgg acgcggccat cgcgcggggg cttcgcatcg cgcccggcga gccgtacatc
     1501 ctcatccgcc gcctgcgtct ggccgacggc gtgccgatgg cgatcgaaaa taccgcgctc
     1561 aacgcctcgc tctgccgcgg cgtccttgat accgacctga aaaaccactc gctctacgcg
     1621 gcgctcacga gccgcggtct tgcgctcaag accggcgagc agtacatgga agccgcgctt
     1681 gcagacgcgg cgcaggccaa acttctcgat attccggagg gcgcgcccgt gctgctcatc
     1741 gagcgccacg tcacaaaccc cgagggtgtc accgtggagg tgacgtattc ggcctatcgg
     1801 ggcgacagct accggttcta catcgaattc gacggcgtga accgggccaa caccgtatcc
     1861 acatccccca aagggatctc ggataatatt atttaaggaa gaaaaaggag cgtcaacatg
     1921 aaaaagatcc ttgcactgat ccttgccctc gttatgctct tcgctctcgc cgcctgcggc
     1981 cagcagcccg ctgcgcagga gccgaccgaa gcgcctgccg agcagcccgc ggaacagccc
     2041 gccgagcagc cggcggaaga ggagcccacc cccgatacca tcaccgtgat ggttcccccg
     2101 gtttccggca cctatcagga cgacatcgac acctggatcg ccgagttcaa agagctctac
     2161 ccgcacctcg atgtggaagt gatcaagacc tcctgggacg atcacaacgg caagctctcc
     2221 accatggcga acgccggcga agctccggac atcgctgagg tctcctactc cgccatcggc
     2281 acctacgtcg agctgggcgt cgccgttgac attgcgcagt acatggaccc tgagcgtctg
     2341 gccgactacg atcagaacgc gctcgactac atgagcattg agaacaccgt ttacggcctc
     2401 ccgctgtaca tcaccatcca ggctctcggc gccaacaagg atatgctcga ggccgccggc
     2461 gccgacgtgg cgaagatcca gtccgaaggc tggacctacg aggagttcct cgaagtcatc
     2521 aagaacggca ccaccgatga ctgcttcggc ttcgtgttcg ccaactccgg cgtcaccgcg
     2581 tccgacttcc tgaacatctt cggcgtcgcc gcgggtctga acaacgcgtt caccgacgat
     2641 ctcaagtatg cctacacctc cgagaacatg ctcaagctgc tcgaggccgt ggaagagatg
     2701 accaagtccg gctacatgcc caactacggc atcgaggccg gccagcgcat ggtcatgtgc
     2761 cagcagggca aggccatgat cttcggcaag gccatgccgc tcttcgagaa caacatcaac
     2821 aagaacaacg ccgctcttga ggccaacgac ggcaccgccg tggagaactc catccccgtg
     2881 aactacgcgt tcctgcccgt tccgacgatg gaaggcgcgg aagcctcctg cttcggctcc
     2941 gtggacggcc tgatcgccct ccgcaacaac aagaccaccg acgagcacct caagaacgtg
     3001 tgcctgttcc tcgactacat ctcttccggc gagcgcatcg ctgccgttga ccagaccctg
     3061 cttctcgagc ccgtctgcca gaccggccgc gacgcttacg tctcccccga aggtcttgac
     3121 gagggcaacg ttgcctccgc cgcccgctgc atcggccttg tcgttgcgcc tcccgccggt
     3181 gtgaccgccg agcagagcgc ttctgccaag accatcatgg acgaagtcat cgttccgaag
     3241 ttccaggctc tccttgccgg cgaagccacc gcgcaggaag tctatgacgc tgtctgcacc
     3301 gccgctaccg aggcgttcgg cgccgacggc tgcgtctccg gcgctctcta agcgttggtt
     3361 tatctccgca aataacgcat aaccactctg ccccgcccgg catggccggg cggggcaact
     3421 cctttcatcc cgaagcaatg cttcgggatg aggtggctgc ggctcgccgc agcgcactcg
     3481 ctgcgagagc acgtttgcga gccgagagga attttttccg acgcacatgt cgccggagaa
     3541 aatgatctca tttatttgct cctgcgggag aaaactctgc gaggctttga taaagccgcg
     3601 tttgattcga ttcgtggagg aaagactatg agcaaagcca gaggaagaac gctcgccgat
     3661 cgccggttcc ggttcaccag cgaggatcgg tggggctatt tcttcatatt tgtcgccatg
     3721 gtggtgttcg tgacgttcac gctctacccc gtggtcagcg ccgtgcgcac gagctttctg
     3781 aagtacaagc ccttcggctc ggaattcatc gggctgaaga actatatcga caccttcaaa
     3841 agcgcgctct tttataaagc ggtgaagaac accattgtgt acacggccgt cgtggttcct
     3901 ctgtcgctgc tgatcgcctt ctccatcgcg atcatgatat tgccgttcaa aaagaaaacg
     3961 cagtcgcttt tcaagtccgt ttactatctg cccggcatcg cctcgggcgt ggcgctctcg
     4021 gtcgtgtggc tgtggctgta cgactcctcg ccggacggcc ttttcaacca ggtactgatg
     4081 ttcttcggca tcccgtcgca gaactggctc tccagctcga aaacgtccat gctctcgctg
     4141 atgctcatgg cgatcctctc cggacagggc agcaacatca tcatctacat agccgcgctc
     4201 ctcggcatcg acaacagcta ctttgaagcc gccgagctcg acggcgcaac gttttttcag
     4261 aaggtgcgct acatcgtgtt cccccttgtg aagccgacga cgctctttct gctcgtgacg
     4321 ggtatcatcg gctcgttcca ggtgttccag aacgccttta tgatgaccgg cggcggcccg
     4381 gacaacagca cgacgatgat cggccttctc atctacaaga acgcctttga gtactcaaag
     4441 ttcggtctcg cctgcgcgca ggctatcctg cttgccatcg tcattgcgct catttcgctc
     4501 gtgcagttca agctcatggg cacggacgtc gaatactgaa aggaggaacg gcatggctac
     4561 gacaggactt tactcccccc acgtaaacca ccccgaaaag cgcatcgcgc gcaacatcct
     4621 ccttgcggtg atcctcatcc tcttcgcgct tctgacgctt ttcccgatct tctatatgat
     4681 catgtcgtcg ttcggccccg ccgtcgccac ggcgggcact tcccggagca tcttcccgcg
     4741 cgtgtggacg ctcgattcgt acaaggcgtt cttttcgttc agcaaatact ccgtccgctg
     4801 gatcgtcaac agcctcatcg tcgcctcggc aacggtggcg ggcaacgtca tattcgcgtc
     4861 catggcgggc tatgcgttct cgaagatccg cttcaagggc cgccagtggc tgttcgggct
     4921 gatcctcgtg gcgatgatga tcccgtacca ggtcacgcag gtgccgctct acattctcat
     4981 tgcgaacaag ctgcacatga cgaacaccta tgccgcgatg atcctgcccg gcgttgtgac
     5041 atcgtacaac atctttctcg ccaagcagtt tttcacgagc atccccacgc cgctcatcga
     5101 atcggccaag ctcgacggct gcaaccagct cggcattttt gtgcgcatca tcctgccgct
     5161 ctcgaaaacc gtgctggcgg tcatggcgat caacacgttt ttgtccagct ggaacacgtt
     5221 cttctggccg ttccttgtaa cgagcaagga ctatatattc acgatccagg tcggcctcaa
     5281 gaccttcaag tttgcgaacg agacgctctt tgcccccatg atggcgggcg cgacgctctc
     5341 ggcgctgccg atgttcattc tgttcttcac gttgcagaaa tacttcctcg agggnnnnnn
     5401 nnnngaactg ctgctcgcac acccggcctt ctgcggcgag agcacgttcg tgctcgaaga
     5461 aaggggcgcg cttcgcggct ttgccgcggg ctgcacgggc gatacgctcg cgcagggcgc
     5521 ggtgcgcggc tatgtgagct gcgtgctgct tgatgagacg tgcgacacgg cggagaacac
     5581 cgcgctgctg ctcggcgctg tggaggattc cttccgtgcg aagggaaaga gcgtggccgc
     5641 cgttacgttt ttcaatcccc tgcgtctgcc gtggatcatc cccggaacgg acggccaccg
     5701 gcacaacaac atgcccggca tagcgaagga tataccgctt tatgagcgga tgctcgcgct
     5761 cggctaccgc gaggcggcga ccgaaatggc gatgtatctc gatcttgcgg cgtttgcgta
     5821 ccccgccgca atggaagaac gcgcggcgaa aatggccgcg cagggctaca ccgtagactg
     5881 gtataaagag ggcgtccacc gcggcgtgga cgagatggta gcgtcgctcg gaaattccat
     5941 gtgggacgcg gagatccccg ccgcggcgca cggcggcatg cgccttctcg tcggtctctc
     6001 cggcgacacg gtcgcgggct tcaccggccc ggtgtacccg gagccgacag gccgcgggta
     6061 ctttgcgggc atcgccgtgg aggaacgata ccgcggccac gggctcggca agcttctctt
     6121 ctaccggctc tgccgcgcgg agaaggaatg cggcgcgcag tatatgtccc tcttcaccgg
     6181 aatcgacaac ccggcgcaga acatctataa ggaagccgga ttcacgccgc ggcggtattt
     6241 cgccgtgatg ataaaggagc tgaagccatg agcaaaacga tattggccgt cggtgcgcac
     6301 atcggcgata tggagctgac ctgcggcgcg ctgctcgccg cgaacgccgt gcacggggga
     6361 cgcagcgtga cgcttgcgct caccgccggg gagaagggcg cgcctgccgg agcagacgtc
     6421 gccgaatacc ggaaaagcaa gatcgccgag gcggaggcct tcgccgccga gctcgggggc
     6481 gaggcgatcg tgctcggcta tccggacggg ctgctgccgg ataacgacga ggcgcgcttc
     6541 gccgtgtgcg atgtcatccg ccgcgtaaag cccgatattc tcatcaccca ccacgcccac
     6601 agcatgcaca aggaccacgc ggcgtgccac cgcatcgtga cggacgcctg gttttatgcc
     6661 gccatcgagg gctttgagcg cgatcttccc cgccactttg cccgccatct gtactatgcg
     6721 gaaaactggg aggatgccga gggcttccag ccctatgtct atctcgacgt gtccgacggc
     6781 ttcgcactct gggaaaaggc catcgagcac cactggttcg ccgtacacag cacgagcttt
     6841 ccctacaagg aatactattc ccatctcaag cgtctgcgcg gtatcgaggg gcggaagggg
     6901 tactgcgagt gctttatgat ccccgccgag cagatgcgca tcgtcaaaaa actggaggat
     6961 gagtgaatga ccgatatcgg aaaactggcc accgagcagc gcaacgccgc ttcggagcac
     7021 atcgaccgtc tgccgacgct cgatatggtc cgtctcatca ataacgaaga taaaaaggtc
     7081 gccggagcgg ttgagacggt gtgcccgcag atcgccgaag ccattgacgg catctacgcg
     7141 cggatgcagc gcggcgggcg gctcatctat accggctgcg gcacgtccgg ccgtctcggc
     7201 gtgctcgacg ccgccgaatg ccccccgacg tactcgaccg acccggagcg tgtccgcgcg
     7261 gtgatcgcgg gcgggtacgg cgcgatgttc gccgctgttg agggcgcgga ggatgaccgc
     7321 tctctcggtg caaacgattt aaaggcgctg catctgacgg aggacgacag cgtcgtcggc
     7381 attgccgcga gcgggcggac gccgtatgtg ctcggcgcgc ttgactacgc gaaaagcgtc
     7441 ggcgcgctca cgatcgcagt tacatgctgc cccggctgcg aggccgaggc gcatgccgac
     7501 attgccatct ctcccgcgcc ggggccggag gtcatcaccg gctccacgcg catgaagagc
     7561 ggcacggcgc agaaaatgat actgaacatg ctctccaccg gcgtgatggt gaagctcggc
     7621 aaggtctacg gcaacctcat ggtggacgtg aaggcctcaa acgaaaagct gcgcgagcgg
     7681 tgtgtgcgca tcgtgcgcca ggcgaccggc gcggaggacg ccgcggcgcg cgcggcgctt
     7741 atcgagacga actacgcctg caaaccggcc atcgtcatgc tgctgctcgg cgtggacaag
     7801 accggggcgg aaacgctgct cacgcgcgcg aacggccgtg tcgccgcggc gctggagggc
     7861 tgagcatggc gtttcttcat tgtgagatcg cctcggaatc gctgcgcatg gcgacggcgg
     7921 tggacgtgat attgcccgat aagggcgagc tttccgaggt caaaacgctt taccttctgc
     7981 atggtctgac ggacgactgc accggctgga cgcgctatac cgccgtggag cggtatgcgc
     8041 gcgagcgcgg tctcgccgtt gtgctgccgg aggtgcagcg cagcttttat accgatatgg
     8101 catacggcct gccgtatttc acctatgtct ctgaggagct gcccgccgta tgccgccgaa
     8161 tgttcggcct tggcgcggcg cgggagcaga attatatttt cggcctttcc atgggcgggt
     8221 acggcgcaat gaaatgcgcg ctcacgtatc ccgaccgcta tgcgggcgct gcgagctttt
     8281 cgggcgtctg tgatctgcgc cggacggtcg agcgccgctc gatctccctg catgaatcgg
     8341 agatcgctgc cattttcggc gaagaaaaag ccgttccgcc gaaggacgat ctcttcgtcc
     8401 tggcggaacg gctctccgga gaggctccgg gactgtatct gtcctgcggt gagcaggacg
     8461 ggctgtacgc ggcaaatgag cgcttttccg cgcatctcgc gtcgctcagc cttgcgcacc
     8521 ggtttgacca ttggccgggc gcgcacacct gggacttctg ggatacatcg ctgctcgcgg
     8581 cgctcgcgta cttttttgat taaagcgtga tcttccccag caccgcgccg tgccgcgcgc
     8641 cggtggcgcc gggcgcgttg ctgcaccggg cgaagatcgc ctcgtttgcg agcgccgcga
     8701 acgcgaccgc ttccttggcg tcgccgtcaa agccgagatc ctcgttcgtg agtacgcgca
     8761 catcggggag cgccgcggcg atatccgcca tgagtgtgcc gttgcggctg ccgcccccgc
     8821 cgacgatgag ccgcgcgggg cgcacggcgc agaagtcgcg gatcgccgcg gcgatcgtct
     8881 gcgcggtgaa gtccgtgacg gtggcgagaa tgtcggcgtc cgagagcttc cgctcgtttg
     8941 ccgcggcaaa aatgccgtcc acgaacgccg caccgtaatg ctcgcggccc gtcgttttcg
     9001 gcgggcggcg ggagagatag ggatcgcgca tgagctgcgc gagcagcgct tcgctcacgc
     9061 ggccggaagc cgccatcgcg ccgttcgcgt catagcgctt ttcgccgttt gtgatgcgcg
     9121 caatggccgc gtcgatgagc atgttgcccg gcccggtgtc gaacgccgtc acgtcggaaa
     9181 gagcgcatcc cgccgggagc agcgtgatgt tcccgatgcc gccgatattc tggagcgccg
     9241 tgtgttcggt ctcactgcgg tagagcagaa actccgtata cggcacgagc ggcgcgccga
     9301 gcccgcccgc ggccacatcg cgcacgcgga aattgcccac gaccgggcag ccgaagcgct
     9361 cggcaagcac tgcttcctcg ccgagctgga acgtgcttgc gaaggtgcgc gagaggtatt
     9421 cttctttttc cggaatgtgc cagaacgtct ggccgtggct gccgatgaga tcgatgtcgg
     9481 agagctttgt gccggtctgc gcgcagagcg cctcgcccgc ctcgcagaaa agctcgccga
     9541 gcaggaaatt catccggcag aagtccgctg ccgaggcagg accgcctccg gcaagacgca
     9601 ggatctccgc gcgcacttcg tccgaaaacg gcacgaagcg gaagccgagc tgctctgcgc
     9661 gcatttgcgt gccaaagccc gtgatgcgaa cgagtgcggc gtccacgccg tccgccgatg
     9721 tgccggacat gaacccgatc acgagccgcg acggcttttc ccacagcgtt ttcaggggat
     9781 gttccataag gatgagacct cccatttttg ctataaacta tgaaaagttt acttcctgtc
     9841 cgccgctttg taaagaggtg tttttatgat ccgattcggc attgacagcg cggagcttgc
     9901 cgcgcatatc cccggccgtg ccgcgctcgt caccgcgccc tccggccgca gcgccgataa
     9961 ccgcagcagc atcgatgttt tgaaggaggt ctgcgatctg cggctcctgc tcgcgccgga
    10021 acacggcgtc cgcggcgaca aggccgcggg cgaggtgttc tccgacagcg tcgacgcgcc
    10081 ctccggcctg cgcgtaaaga gcctgtaccg ccccggctcc aagcgcctga cgcgcgaaac
    10141 ggcttcggaa tttgatacgc tcgtctacga tattcaggac gtcggctgcc ggtactatac
    10201 attcatttcc acgctcaaaa atctgctgtt cgactgcgcg gaatacggta agcgcctcgt
    10261 tgtgctcgac cgtccagacc ctctgggccg caccgccgag ggcgtcgtgc tcgagaaggg
    10321 catggagagc ttcgtcggct gccatacgat cccgccgcgc tacggcatga cctgcggcga
    10381 gtttgcccgc atggtgaatg cggaggagca tatcggctgc gcgctggaca ttgtgcgctg
    10441 cgaggggtat gatccccgtg tgagcttccc cgactggggg cggccgtggg tcatgccgag
    10501 ccttgcgatg ccgcggtatg aaacggcgct gctgtatccc ggcacctgcc ttctggaggg
    10561 aacgaacctc tccgaagggc gcggcacggc ggacccgttc gcgctgctcg gcgcgccgtt
    10621 tatcgacgcg gaagctctct gccgggagtt tgacgctctc gcgctgccgg ggctggctgt
    10681 gacgccggcg tacttcacgc ccacggcctc caagcacgcc gggacgctct gcggcggtgt
    10741 gcatctgcac gtgatggatg ccgcggcgct ccgcccgacg gagctcggtc ttcggctgct
    10801 cgcgctcgtg cgggacatgt accccaaaga cttcgccgtt ctcccgcccg tgcgggcgga
    10861 cggacggccc ttcatctcgc tgctcgccgg gcaccgggaa ctcgagcacc ccggctggga
    10921 cgccgagacg ctcatcgcgc acgcccggcg cgagtgcgcg gcattcgccg agcggcggag
    10981 accctacctt ctctacgaat aaggaggaaa atttatggac attcgggaaa aaatcggaca
    11041 gcggctggtg ttcggcttcc ccgggacgac ggtcccggcg gagtttgccg cgctggtgcg
    11101 cgaatataag atcggaaacg tcatcctctt ccggtacaac atcgaaagcc tgccgcagct
    11161 cgcgcggctg tgcgccgata ttcaggagct tgtccgcggc gcgaccgggc gcgatgcctt
    11221 tatcaccatc gaccaggagg gcggcatggt gacgcgtctc ccgtgggacg cggtgaacgt
    11281 ccctggcagc atggcgcttg ccgcgaccgg aaagccggaa aatgccgttc tcgccgcgga
    11341 gatcacggca cggcagctgc gcggtgtcgg cgtgaatttc aatcttgcgc ccgatcttga
    11401 cgtaaactcc aaccccatga accccgtgat cggcgtgcgc agcttcggcg acgatccgga
    11461 gcgggtggcg ctcttcggcg cgaaggccgt ggagggctat aaaaacggcg gcatgctctg
    11521 ctgcggcaag catttccccg gccacggcga caccgccgtg gattcccaca tcgggcttcc
    11581 ctgcatcgag aaaacgatgg acgagcttga agcgtgcgag cttatttcgt tccgcgcggc
    11641 gatcaaggcg ggcattcccg ccataatgag cagccacatc ctcttcccga acattgagcc
    11701 aaatggcgtt ccggccacaa tgtcgcgcac gatcatgcac gaccttctgc gtgaaacgct
    11761 gggctttgac gggcttattc tcagcgactg catggtaatg gacgctatcc ggcggcatta
    11821 cggcacggcg cacggcgtcg tggaggcgat gcgcgcgggc gtggacatgg tattcgtttc
    11881 gagcaatgcc gatctccagc gcgagagcgc tgccgcggcg ctcgccgccg ccgagagcgg
    11941 ctcgatcgac gcgcgggagc tggacgggtc tgcggagcgc atcctccgtg ccaaggagcg
    12001 gtggatacat cccgccgagc cggagagcgc cggccgtgcc gaggacttcg ccgctgccgc
    12061 gcgcatggcg cgcgaggcgg tcactgcggt gcgcggcaag gcgtgccggg caagcgaaaa
    12121 gacgtttttc tgcggctgcg ccgattaccg catgacgcag gcatcaaacg ccgacccgga
    12181 ggcgaaagcg ttcccggagt atatgggcgc gcgtttcgga gcgaaattcg ccgtctgctc
    12241 caaggacccg gacgagaagg agatcgcgga tctcgtcaaa tgcgcggacg gagcgggaaa
    12301 tatcatcttt tcctcctgca acgcgcacct cttccgcggt cagctcgctc tcgcggcggc
    12361 gctcgccgaa aagggcgtgc cgatgaccgt ggcggcgctt cgcaacccct acgatcttcc
    12421 gctcatgccc gaaaatgcgg cgctgctcgc ggcattcgat tattcaagag attctctcgc
    12481 cgcgctcgcg gacgtattct ccggcggaga ctgctgcggc gtgatgcccg cggcgctgtg
    12541 aggtgagaga tatgtttgtt gtcggtattg acggcggcgg gacaaagacc cgcgtggccg
    12601 tctgcgcgtc ggacgggacg ctgctccata gagaaacgct cggcgctttc aacctttccg
    12661 ccatcggcga ggacggcttc cgccgccgcg tgggggagat cctcacgctc tgcggcgata
    12721 tgcgcgcatg cggcgcgctg tgcgtcggcg gcgcaggagc gtccggcgcg gcgatggggg
    12781 agatactgcg cgcggagctc gctgcgcacg gctttgccgg aaagctcctt ctctgcggcg
    12841 accatgagat cgcgctcgca ggcgcgatgc agacgccggg gtgcgttctc atcgcgggaa
    12901 cgggctcgat ctgttacggc aaaaacgccg ccggagagca ttttcggtgc ggcggcggcg
    12961 ggcacatcat cgacgatccc ggcagcggct acgcgctcgg ccgggacgcg cttgccgcgg
    13021 cgctccagac cgaggacggc cgtctttcgg aaaatgcgct ccacggcgcg gtgatggaca
    13081 ctgtcggcgg cagcggcatt cagggcatat tcgattttgt ctatttctcc cgtcgcggca
    13141 agggcgacat cgccgcgctc gcgccgctcg tgctgcgatg cgcggcgcag ggggacgcgg
    13201 tcagccttga tattctccgc cgcggcgccg ccgagcttgc ccgcctcgtt tcggcggtca
    13261 tgggaaaact cggtcttgaa aacggctcgc cgtgcgctct cgcgggcggg ctgctcgcgg
    13321 aggacaacgt gtaccgccgc gcggtgtgcg cggcgctcgc accgttctgc cgtccggcgg
    13381 ccccggagca cgacgcgctc ttcggcgcgg tgcagctcgc gctcgccgcg atacagtgnn
    13441 nnnnnnnnga acagaaaaac ccggctcttc ggaaacaatc ggaagagccg ggttttctat
    13501 atgcggtttc agtacagcgt ctcggggtac ataccctcgg cggtgagctt gcggagcatg
    13561 gcggcggtcg tggcgcggtc ctcgtggtac gtcatgccga accacgcttc ctcggtcgtg
    13621 taggcgtcaa cgtgcatcga cccttcgcga agcatgtcac cgaccatcgt cggcagcagg
    13681 cactcgctct tgagctcttt gcccgcctcg gagcggagga acgtatggaa gtaggtctcc
    13741 atctcgtcga gcgcccacgg cgtgaagccc cagaagttca tcgataccgg gctttcgccg
    13801 tcaagcacga cgccctcctc ggaatcggcg aacgagcgga tggagccgtc cggcgccttg
    13861 ccgatcttga acgtctcttt tacggcggtc atctcgccgc cgtcgatgcg gcatacgccg
    13921 cgcgtcacgg tgccaaaatc gctcacggtg ttgcgcagct tgtaggcgac catgcagccg
    13981 cggcccgtct ccgggagctt ttcaagcgct tcgtacatca gcgtgaaggc gccggggccg
    14041 tagtaatcgt cggcgttcac cacagcaaac ttgccggtga tcacgtcctt gccgctgagt
    14101 acggcgggga cggtgccata aggcttcgtg cggcctcccg gaacggcgaa ccaatccggc
    14161 agcgcgtcaa agctctggaa ggcgtaatcc acgtgcgcct ttccttcgag ccgctcgccg
    14221 cacacggcgc ggaagtcctc cagcatctgc ggctggagaa tgatgacgaa ccggtcaaac
    14281 ccggcgcgct gcgcgtcgtg aatggtgtac tccatgagga tctctccgcc ggggccgacc
    14341 ttttcgatct gcttcgcccc gccgtagcgc gaggcgagcc ccgcggccat gataacgagt
    14401 gttgcggaca tgaaagttct cctttcgctg caaaaacgga tgaggatatc aataagcgct
    14461 tatttcaaga cttgcgtcgt cctgaccttt ttcgatggac cggatctcgg cgtaataccc
    14521 gtaatcgtcg ttcacgcgga cgtatacgcg ctcgccggcc ttgtggccga tcaccgccgc
    14581 accgagcggg gattccttgc tgatgagtcc ttcgagagcg ttctgccgga gcgttgttac
    14641 gaaccggacg gtcatctcct cgtcgtcatc ctcgagatac agcacgacgc ggtcaaagag
    14701 accgacctca tcggcgccgg actttgcgtc gatcacaacg gcggaggcga gcatctgctt
    14761 gagatagcgg atgcggctca tgttcttgcg ctgctcggcc ttggcagcct tatactcaaa
    14821 attttcactc agatcgccga acgagcgggc gaccttcaca tcctcgagga tcttcggcat
    14881 gagcacttcc tcgcgctcgc ggagctcctc gcgcattttc tgcatatcaa cggcagtcag
    14941 ttcgtcatgc atggtgttta tttggccttc tttccgttaa agcaccgccg gatgctctcc
    15001 ttcgtgggga agaggaacgg cgtggtgtcc ttgtaggcgt tgtacccgcg catgagcacc
    15061 gggaaaacat tgacgtcctc atacattgcc agcagcagac cgcacaggct gaaagtggtg
    15121 gcgcaccacg gatcaaagtt cgccgccgga atgagcagca gacagaaaaa gagcatcgcc
    15181 caaacactcg gatggcggca gagagcgtag gctcccgtcg tcacgagatc cttttcccac
    15241 cagcgttttt tggggtcctt gcgcttccag ttcgtgcgcc cgccggcgat gagggtgagg
    15301 tatatgagca gcgcgaaaaa gccgagcgcc agtacaccgg cgatgatgcg taccgctgcg
    15361 ggcagggaat tggcctccga aaggccgggc aggacggtgc tgaaaaacgc gagaccgatc
    15421 agaccggcac cgacggtgtg gagcgactgc atgcggtcgt tgttgtgtga ggtattgttg
    15481 agatcgtaga gaatgatgct gacatagccc agcacgccgc agacgatggg gctcaaacca
    15541 tcaaagagca taggggttct ccttttctat caaggcggat ttatccctat tactatacca
    15601 tattctgccg gagaaaagca atacgggatc aaagtatctc gtccggcacg ctgcgggcga
    15661 gagcgcggtt ggtgtagcgc ggatcgtccg tcacttcacg gtatacatgc agccagtccg
    15721 ggagcggcag cggctcgtcc tcgctttgca gctcgcattc gc
//
LOCUS       DAWAAE010000030        15516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1715_length_15516_cov_3.121467, whole genome shotgun sequence.
ACCESSION   DAWAAE010000030 DAWAAE010000000
VERSION     DAWAAE010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 15516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15516
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_15516_cov_3.121467"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2695..2704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6381..6390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8686..8695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3213 a         4577 c         4398 g         3298 t
ORIGIN      
        1 tatgagggca tttccgcccg gcagaaccgc gtgtgccctt gtcaatcgac gcaaattgac
       61 agaaagggag aaaaccacca tgaaaaagac ctacaagatc gaagtggact gcgccaactg
      121 cgccaatctg atggaggacg ctgccaagaa gaccgagggc gtcgcggacg ccgttgtgaa
      181 tttcatgacg cagaaaatga tcgttgagtt tgccgagggc agcgatccca agaccgtcat
      241 gcagagcgtg ctgaaggcct gcaagaaggt cgagcccgac tgcgagatcg aactttgatc
      301 cccgccgcat ccggcggcgc agtttccaga caggaggcgt aaggccatga caaaaaaaca
      361 gaaaaagatg ctctggcgga tcgtcattac cgccgtgatg ctcattgcgc tgcattttct
      421 tcccatcacc ggcatagcgc agctcatcgc atacctcgct gcctacgcca tgatcggcta
      481 cgacatcctg cgcaaggccg gcaaaggcat tgcgaacggc cagccgttcg acgaaaactt
      541 ccttatggcg cttgccacgc tcggcgcgtt cttccttgcc atctggacga agagcggcga
      601 ttacgtcgaa ggcatcgccg ttatgctgtt ttaccagatc ggcgagctgt tccagagcta
      661 cgccgtcggc aagagccgcc gcaacatctc cgcgctcatg gatatccgcc cggactatgc
      721 gaacatcgaa acggacggca agctcgagca ggtcgatccc gacgaggtcg ccgtcggcag
      781 cgtgatcgtt gtgcagcccg gcgagaaagt gccgctcgac ggcgtcgtcg tcgagggcga
      841 ggcaagcctc aacacgagcg cgctcaccgg cgagagcctg ccgcgcgacg taaagaccgg
      901 cgacgagatc atcagcggct gcatcaacat gaccggcgtg ctgaagatcc gcacgacgaa
      961 ggaattcggc gagtccaccg tttccaagat ccttgatctt gtggagaacg ccagctcgcg
     1021 caagtcccgt tccgaggcgt tcatctcccg ctttgcgaag gtgtatacgc cgtcggtgtg
     1081 cggcgcggcg ctcgtgctcg gcatcgcggt gccgctcctc cggcttgcct tcggcgctgc
     1141 cccgaactgg acggactgga tctaccgcgc tctcacgttc ctcgttgtga gctgcccgtg
     1201 cgcactcgtc atcagtattc cgctgagctt tttcgcgggc atcggcggcg cgagccacga
     1261 ggggatcctc atcaagggct caaactatct cgaagctctc tcggacgcga agtatctcgt
     1321 gtttgacaag accggcacgc ttacgcaggg cgtgtttgaa gtgaccggcg tgcaccacag
     1381 cacgcttgaa gaagcggagc ttctcgaata tgccgcgctt gccgagtgcg cctcgtcgca
     1441 cccgatcagc aagagccttc agaaggccta cggcaaggag atcgaccgca gccgcgtctc
     1501 cgatatcgag gagatcagcg gccacggcat taccgccgtg gtggacggcc acaccgtcgc
     1561 cgcgggcaac gccaagctga tgaaaaagct cggcatcgaa gccgttgaat gccacagcgt
     1621 cggtacgatc atccatatgg cgatcgacgg gcagtacgcc ggccatatcg tcatctccga
     1681 tgtgccgaag ccgcacgcaa aggaagccat cgccgcgctc aaaaaatgcg gcgtgaaaaa
     1741 gaccgtcatg ctcaccggtg acacgaagcg cgtggccgag caggtagctg ccgagctcgg
     1801 cgtggacgag gtccgtagcg agcttctccc cggcgacaag gtcacggagg tcgaaaagct
     1861 cctcgcagcg aaagggccgc acgagaagct cgccttcgtc ggcgacggca tcaacgacgc
     1921 gcccgtgctc acccgtgccg acatcggcat cgccatgggc gccatgggca gcgacgccgc
     1981 catcgaagcc gcggacgtcg tcctcatgga cgacgatccc aagcagatcg tcaaggccat
     2041 ccgcatctcc cgtaagtgca tcggcattgt caagcagaac atcgtgttct cgctcatcgt
     2101 caagttcggc tgtctggcgc tcgtcgcggc cggcattgcg gacatgtggg cggcgatctt
     2161 cgccgacgtc ggcgtcatga tcctggcggt cctcaacgcc atccgcgcgc tgcggtataa
     2221 ggaataatcc gatgacgagc agacccggca aaagaggaca ataaaagaag atcccccgga
     2281 tgcagtactt ctgttttctg catccggggg aatcttgtct gcggctttgc cggtttattg
     2341 tctgagcgtc tcttcgagtc gcgggatcga cggaacggcg ccgggacgtg ttacggcaat
     2401 cgccgcagct tttgctgcga tctccatgga gtccttcggc gtgccgccgc gcagcagcat
     2461 ggcgagaaaa tacccggtga acgtatcgcc cgcggcggtc gtgtccacca cggtcacgcg
     2521 gtgtgcgggg acggagaaac tctccgtttg gtctgcgtac attgccccgt cgtgtccgag
     2581 cgtcaggacg gtcgcggcgc caggaaaccg gcggcgcagc gcgtcgagga ccttttccgg
     2641 cacgctttcg ccggagaggg cgccgccctc gatctcattc acgatgaaaa tatcnnnnnn
     2701 nnnncgagcg gccacgagag gatctccgcg gaaaacggcg aggggttgag cgcaacggtc
     2761 agaccttttt cgtgcgcggc gcgcagcaca tccggcatat gggagatctc attctgcgtc
     2821 aggagaacat cgcccgtacc gtagccgtca agaaaggccg cgatatcgtc cgtgcccact
     2881 gttccgtttg cgcccggctc gacgataatg cagttttgcc cattggcgtc gacttggatg
     2941 atggcgtggc ccgtagtgac ggaacgcgca cggatacgcg cggcatctac cctagcttcc
     3001 gcacagaccg ccacgagcgc ctccaattcc gccgcgccga cgcagccggc gagcgccgtt
     3061 tccgcaccgc cgcggctgag tgcgatcgcc tgattgagac ccttcccgcc ccaggcggta
     3121 cggtattttc ggctggagac tgtttcgcct ggacggacga tgtgctcaag ctcatagaca
     3181 ctatctatgt tgatcgaacc gataacgaga gctcttttca taatgtgctt cctttcctgc
     3241 gcgctcgtgc gctttctctt ttgataagcc ggggttcaaa gtatatggac tgatgcggca
     3301 gctccggatt ggcgatctcc tgcatggcac ggctgcatgc ctcatacccg atgtcgtagc
     3361 cagactggcg gattgttgtg agcggcgtat ccagcatttc gcaaaagctg atgtcatcaa
     3421 aacccacgac ggaaatatcc tgtccaacgg taatgccccg ctcgcgaaac cgccggtaaa
     3481 gcccgatggc ggtcatatcg ttttcacaga atacggcggt gaaccctccg gcaatcacgc
     3541 tgtccgccag cgcataccct ccggtggaaa gatagtcgcc ttctatgacc atgtcgccgg
     3601 tcacggagat cccagcctcc tccatggccc aacggtagcc ggattcgcgc tcctgcgaac
     3661 tcatgagcca gcgcggtccg gtgatgcatg cgacgcggcg atgtcccagc tcaatcagat
     3721 gcctcgcggc gagatagccg cctttgcggt tattcagccg gatggagctg tacggcagcg
     3781 acggtgactg ccggtccacc tggatcaccg ggcaggacat gccggcaaac acatcgggcg
     3841 aacaattgta ggaaagggca acgatcagcg cagacacgcc cgttgcagcc atggagcgga
     3901 tctgctcggc gtcacggctc ggcgactcgc cgttcgttgc gaggaggacc ttggcctcct
     3961 gcttataggc gcagtccgtc atcccctgca cgatgtctgc gaaaaaggcg ttggacagct
     4021 ccggaacgat cagcccgaga atgaccggct tctgccggct ttttcgtttt ccgctcggca
     4081 aatagccgac gcgctctgcc gcttcaaaga tgcgccggcg gttttccggg gaaatgcggt
     4141 tttccctgtg gttcagcacg agtgacacgc tgctgacgga caggcccaac tcctctgcga
     4201 tactgcgaag cgtcggtttt tccttcatat ggccgcttta ccacccttcc attatagacc
     4261 tttcactttg attatatctg aagcggcgaa aaaatcaagg taaaattttg attttttatc
     4321 acattttttt cggagttttt acacgttgct gctgctgttt attgttcgat aatccaattt
     4381 ttcaatttat gatcgacttg agattgaccg attcgtgaat aaatgatata aaaataagca
     4441 gaaggtcaca aaccctttat tttgtgttta tgataaaaag gtgataaaat gtataatgac
     4501 gctgttttaa atggagacct gaatcgagtt ctgggaaaca tgcggcacac ggatcggctc
     4561 tgcatcgcag actgtgggct tccgatcccg gatggcgtgg aagtgatcga cctttcgctt
     4621 aaaatgggcg agccgtcgtt tctcgcagta ctggatgttg tgctcgagca cttcggcgtg
     4681 gaaaagatat ttctggcttc ggagacggag aacatgaacg gtgcgctcta tgaacagctc
     4741 caaaagcgtt tttctgctac ttcctgcgaa acgatgccgc acgaggattt taaaaaactc
     4801 tccgccacct gccgcgccat cgtgcggacc ggcgagaaca ccgcctatgc caacctgatc
     4861 ctgcagtcat cctgcatttt ctgaaattcg tacttcgccg agcgatcggt aaagataaat
     4921 atgaattatc tggaggagaa gacatgaaaa caaagaaatt cttcgccctg cttctggctc
     4981 tcgccatgct gctggctctt gccgcgtgcg gaaacgcttc tgcggacacg aatgagacca
     5041 acaccaatac cggcgcgaag cggaagctgg ttgtcgccaa ctggcagtcc tacgccacgg
     5101 actccgacta tggagcggcg cacttcgccg agctctatga ctgcgaggta gagcactatt
     5161 tcctcagctc catccccgaa ctgctctctg tgctggagaa cggtggcgtc ggtcagatcg
     5221 atgtggtctg cataaacccg ctttatcttc agcagtacct gaacgccggt gtgctcgaca
     5281 cgattgacac ttccgcgctc gagaattaca ataaccttcg cgaggacttc cgcacgatcg
     5341 acgaagtcaa ggacgaaaac ggcaatatca tcggcgtccc ctgggtttgg ggtacgacct
     5401 ctctcttcta caacgcagat gtcatcaccg acgagatcac aagctggtcc gctctctgga
     5461 atccccagta tgccggtaag gtcgcctttg aaaacgagta tgacgccgcg atccgtacgg
     5521 ccgccatgta caccggcgag gatccccgca atccggatac cgacaccgtg cgcaccgctc
     5581 tgaccgacct caagagcaat gtgaagacct actggacgag ccatgacgac ttcgtcaagg
     5641 cctacacctc cggtgaagtt gtgatcggca acgtctgggg ctctattgcc accgagctca
     5701 aaaacgaggg cttcaacatc cagtacatcc atcccgaaga aggaacggtg ggctggtgtg
     5761 attattggtg catcgtccgc gactctgcca acaaggatct cgccatgaaa tggattgact
     5821 actgcacggg cgagtacttc cagaacagca tggccacggc cggcgcgcag gcttattctc
     5881 ccgttaacac ggttgtcacg gaggccctct ccgacgaagc gcgtgcttcg ctttggctct
     5941 accccgaagc gcccgcaaat gtcaacctgg ctcttccgct gaccgacgag cagctcacgg
     6001 cgtggacctc cctctgggat gaagtcaaag ccgcaaaata agtttttccg tacgggccgg
     6061 ccggtctcct ggccgacccg tatacctctt ttgtaactgg aaaggaatca cgccatgaaa
     6121 ccatccaagc cccgtagtgc cgcccggcat ctgatctggc cgtccgctgc catactgctc
     6181 ggcatgagtt ttgtcccgct gtgcatgctg gtatttttca gctttgccga cggcaacgta
     6241 tccacgctca acggtgtcgc ggggtacacc gtgcgcaaca tcgtggaggc cgttacctct
     6301 ctcacggtgc aaggcctgat gctgaaatcg ttctggatgg ctcttctcgt aacggccatc
     6361 tgcattgtaa tcgcttatcc nnnnnnnnnn ctattctatc atgacgctca tgcagacgaa
     6421 cggtcttgtc atgacgccgc tgctgaagct gggcattctc ccggcgggaa cttcgctcat
     6481 gtatacgaac gggtcggtca tactcattct cgtgtatgag tatctgccat atatgatcct
     6541 ttccctgtat tcctgcctgg aaaagatcga caacaatgtt atttccgcct cgcactctct
     6601 cggcgcgaac cgctttcaga cctttatcag cgttgttctg ccgcagagct tctcgggact
     6661 gatgaccggc attttgatcg ttctcatccc cgtggctggc agcttcgttg agccgaacat
     6721 cgctggcggc ccttacagca tgatgatcgg aagcctgatc gactcccagt ttaccacttc
     6781 catgaacatg ggctacggcg cggcgctctc gctgttcttc cttgttgtca tgtcgctgac
     6841 gctggccgcg atccgtctcg tcatgaacat tgcagacaag cggatcggag gataaaatag
     6901 ctatgaaaca aaatccaaaa cgcagcgctt cggcgcggcg gttcaatacc gttctcattg
     6961 tctggctgtg ccttacgttc tttttccttc ttttcccgat cctaggcatt gccatcatgt
     7021 cgttcaactc atctaaatac ggcgcgcttc cgtttgattt tactaccaag tggtacgctg
     7081 aactttttcg ctcggcggat ctgatcagcg ccacgtggta ctcgctctgg ttcagcctgc
     7141 ttgtgagtct ctgcgcggcg gtgctcggaa tcatggcatc tcttgcgctg cggcggatgt
     7201 cacgcaagtg gaacagggcg ttcccgacgc tgatgaacgt cccggtcatc attccctggc
     7261 tcgtgcaggc ggttgcgctg ctgctgctct tcaactacct cggtatcggc aaatcgtttg
     7321 ccagtctctt cttcgggtgc ctcgtcgcgg tcatgccgca ctcgttcctc attacgtatt
     7381 cgcgcattat gacgatggat aaatatccag aggaagccag ccgcacgctc ggtgcgtccc
     7441 ctctgcggat cctgtgggaa gtaacacttc ccatgatctt cccggcggtg ctctcgggct
     7501 ttctcatgtc gtttgtgctg tgcttcaact gcttttccat ccagtattac ctggcgccgt
     7561 tcgggacata tacgctcccg atgctcattt ataccaagat ccgcgccggc tacaacccgg
     7621 acatcaacgc tattgccacg ctgcttacca tcgccattgc ggtcgtgatc tttatcatga
     7681 accgtgtggg cttcgatgcc ggacagcttg ttgggcaggg acataaggag aaataaccat
     7741 gtctgaaatc accattcagg gcatttccaa aacctttggg gatacccgcg ttctgaacga
     7801 tatttctttc tgcgcgaagg acggcaatct tctttcgctg cttggtccct ccggctgcgg
     7861 aaaaacaacg cttctgcgca tcattgccgg gctggagtat gccgacagcg gtacgatcca
     7921 tgtcggcgag cgcgatctca cgcccattcc ggtagaaaaa cgcaacatcg gcgttgtgtt
     7981 ccagaactat gcgctcattc cgcatatgac cgttttcaac aacatcgcct acggcttgaa
     8041 gatccgcaag gttcccaaag agcagatccg ggaaaaggtg gagcagtaca tccgtcttgt
     8101 cggacttacc ggtatgaccg accgcaaggt gacacagctc tccggcggcc agcagcagcg
     8161 cgtagcgctg gctcgagctc tgatcatcga gccggacatc ctgctgctcg atgagcccct
     8221 ttctgctctt gatcgaaaga tccgcgccga gatgcagtat gagatccgcc ggatccaaca
     8281 gcaggttggc attacaacga ttttcgttac ccacgaccag gaagaggcca tgacgatgtc
     8341 cgacgaaatc ctgctcatgc gcaacggcgg catcgaacag ctctctacgc cggagaacat
     8401 ttataaccat ccggtgtcgc tctatgcctc tgactttctc ggcaaggcca acacgctcat
     8461 cggcacactg catcaggccc ctgacggctg gacggtcttc ggtgacggct gggaattccc
     8521 cgttgagcca cagaacggct ttgccgacgg ggagaaggtc tgcctggcga tccggggcga
     8581 acagacccgc attctcgatg ccgcggcgga cggtgcgacg gcggcggaga tcaccgatat
     8641 cgtgtttacc ggcgaaatct gccgcattac cgcccgcgcg ggcggnnnnn nnnnnacgct
     8701 ttctcttgcg tgcatcaacg tgcaggcgga gcactaccgc cccggtcaga cggtctacgt
     8761 tctcgccgac cgatccaaaa cgcattactt caaaaaataa ctttgccgga ggatatgtat
     8821 tatgataacg ccggaacgcg gctggaacac ctggcacccc gtttcctgct cccgtctgga
     8881 aaacctcccc gccggtcttt cgatcgtccc gggcgctttc tctgaggcgg aatcgtcgta
     8941 tacgcagttt ccgtttgata cggaaacgcg gctcttagag catgaatcct ccggccgcta
     9001 ctgccgtatg cgagtcagcc atgcctgcgc ggaattcgag ctggaatacc tgcgctttga
     9061 tgcgtggacg accctcttcc gaatgacaaa cctccgcccg ccgcgggagt ggggtatccg
     9121 gtatcacatg ctcgtttcca tcggctttga gaatggcggc gccatgtacg agacggccga
     9181 tggtcttttt ggccggcggg acaactattc tgccgccgta tcctatgccg gagaaccgcc
     9241 gtatgacgtg atccttgcag gcaccgcaga ctatgccggc ctctgcatgg cggacaaggg
     9301 ctataaatcc acggtatccg tccagaacgc cgagagcgcg tgggcgacct gccggtttgt
     9361 actcgagcag tcgccgcagg tctgcctggc agtgtctctt gcgcacgacg aggatacggc
     9421 acgccgcgcg gccgaccgcg ccgcagcgga gttctctcgc tgggaggagc tgcgcgccga
     9481 cgcgctgcgg gattatccgt gcagcaccga tggcgagcac ccgcacatga ccgaggccgt
     9541 ttcggacatc atggcctgga acgccatgca ctccccggag cttggccgcg cctacaacgc
     9601 catcgccaaa agctggaata aaaatttcgg cggttggtat ctcttcttct ccgacgcatg
     9661 ctatcagatc atgctctcct cgctttcggg tgatccggaa atggctgcgc aggatctcga
     9721 ttatgtcctc tccgccgcaa caccggacgg caatttcacc ggcatgctct cgccgtatca
     9781 gaaatgggta gaccgcacgc agccaccggt gctcggcttc tcgctctgga tgcactatct
     9841 catgagcgaa gacatcgccg ccgtggagcg cgcttacccg ctgctccgcc gtgcgcagcg
     9901 ctggtatctt tcccaccggc gggacgacgc gatcccgctc atccgtctcg gcacctccgc
     9961 caccggcgac ggctcctacc gcggcacaaa gcttgccgct aaaaacgaga ccgccatgga
    10021 caacagcccc atgtacgacg aagcgtcgtt cgataaagcc tccggtcttc ttgaaatgta
    10081 tgatgtcggc gtcagtagcc agcttgcgct cgatctcgag tgcactgctt atatggctac
    10141 actgctcggt aaggagagcg acagggagga gctcgcatcc gccgccgaag aattgcgctt
    10201 cgccatcaac gagcagctat gggacgaaga aaaaggcatc tacgccaacc gcaataccga
    10261 cggaaccttc ggcctaacga gcccgacgag tttttacccc ctggctgccg gcgtggcgga
    10321 cagcgatcgc gtggataagt gcctctctca catttttaat gaggaagagt ttttcacgcc
    10381 gtttcccctc atcgccatca atgcttcgca tccctcgggg catgaaaacc gttactggcg
    10441 tggtcggacg tgggcgccgc agccgttctg gacgtacatc ggcctgcggc gctgcggccg
    10501 cgacacggaa gcgcaccggc tggctgagcg tgccgtgtcc ggttttgaac gccattggac
    10561 gaccgagcgc cgcagctttg aaaactataa cccctttacc ggcgaaggag cggataccgt
    10621 ggattcgcag ccgttctaca gctggacggc gcttttcccg cttatgtggt cgctcgagca
    10681 gttcggcgta acgccgtggg atggcctgtt cttcggcatg ctggacggca cggctttttc
    10741 ccagaaaaac cggctgtacc gcggccgccg gtacgacgcc gtttgcgacg gcagcgagat
    10801 gacgcttttc tgcggcggcg cggagatctt ccgctccaac attcctgcgc gcttccggca
    10861 ctttgtccgg gacgatcacc gcgcgtccgt tacggtcacg tccaacgccg gcggcacggt
    10921 ttccttcccc ggcatcacgc cgcgtctcgc gcttgtaaac ggcgtgccgg ttgctgctca
    10981 aagcacgctt tccgtccccg gcggcacggt ttgcgtggag ctttattact gataaggaac
    11041 ggtaaaataa aggcaggtgc atggataata ccatgcgcct gcctttttgc tataattact
    11101 gcatcaccgt gccgtcggca agagtgatag tgtagccatt gaagatgatg gtgccgttat
    11161 ttacaagact tgtgaccgtg aagtcaccgg tcagcgtcca cacgcagccc gatccgaccg
    11221 ttacgacggc gtgattctct gccgctgcgg cgcaaaggat cgcaagattt tttcttattg
    11281 ttttcaaaag gaatgacatc cttttgggtc tctggtgtaa gcatacggcg gcgtccggaa
    11341 agcagacgga aaaaaccgtc tgccgcagaa aaattttaac cacaagttga atgacaaatg
    11401 gaaaaacgta tggtatagtg catacagaaa ttgaaattca cgaaaggaaa tgcgatcatg
    11461 aaggctattt cttccgccat cttctccatg agcgaacaac aggctgtttt cgagcaggaa
    11521 aacagctgcc gcgaagcgcg tctatggcag gagaagcgag agaaggaacc tcggatcggc
    11581 gacacggtat acccggtgta acgggactgc cgggccacac ttccttcgga gatcgtataa
    11641 gccgcctgcc tggagaaaaa ggcaggcggc tttttgcgcc tgcggatatc tgaacttgtc
    11701 aggaggtgat aaaaaatgga ctttcccgcg atcgatccgg taaagaccgg cgacaatatc
    11761 acaaagctcc gaaaggccgc aggcctttcc gtacacgatg tacaggcggc gtttggcttt
    11821 aactcgccgc aggcgatcta taaatggcaa aacggaacgg cgctgccttc tgtggataat
    11881 cttgtctgtc tcgcagcgct gctgcgcgtc cggatggatg atatcctcat cacagatcgt
    11941 tcctgaattt tcggtcggga gccgatactc ccgcttctat ggtccgctcg tctaacagga
    12001 taggacgcag ccctttcacg gctgtaatga ccggttcaaa tccggcgcgg atctccatat
    12061 atggtgcctt cgtctaatcg gacaggacgc aagcctctca agcttgtaat gccgggttca
    12121 agtcccgcag gcatcaccac cacgtcagaa gaagtcagca ccgttccgtt tccgccttgc
    12181 ggcgaaaact gtactacgct gactccttct tcctttccaa atcgaacccg ctccgccggg
    12241 ctttgatctg ggccttgatt ttataattct gtattggggt gtagccaagc ggtaaggcag
    12301 cggactttga ctccgccagt cgccggttca aatccggccg ccccagccac gccgccgcgg
    12361 accgtgctag tccgcggcga ctttttttat gccgtgcata aaaacttctc tccgccgtgt
    12421 ctcgatggcc ggagggaaaa gttgaccggc tgtgataaaa tgaaaaagaa gggaagggaa
    12481 ggaaatggac gagcggataa agcctgcggc gcactatcat ctgccgggac tttttgagtt
    12541 ttacgaattt taccgcgtgt ttctgccgct gttttatgag catcgggaat ggttttacga
    12601 ttggtgcgac atcggctcga tctacggcgc gccggcggac tgtctctggg acggcggacg
    12661 caccgggtac ggggacgatg atccgcgcga ggtgctctcg ctgctgcgcg aatacggcat
    12721 ttcggcgcgg ctcacgttca gcaattccct cctgcgggag gagcatctct cggataacaa
    12781 gtgcaccggt ctctgcgcgc ttttcaatga gagcgaaacg ccgcgaaacg gcgtcatcgt
    12841 ccattcggag ctgctgctcg cctatctccg gcggcggtat cccaagttgt atttcgtgtc
    12901 ctctacgacc aaggtgctga cggattttcg ggattttcgg aaggaactcg agcgggagga
    12961 gttttcgtat gtcgtgccgg attttcgcct gaaccggagg tttgaccggc tcggcacgct
    13021 cccgcaggcg gaaaaagaca aggtggggtt cctctgcaac gagtgctgcg attttggctg
    13081 cgccgagcgg cgggcatgct acgaagccgt cagccgccaa aacctcggcg aggatgcgcc
    13141 ggagcaccgc tgcgccgcgc ccgacggcgc gcagggctac cggttttcca aagccatgaa
    13201 aaatcccggc ttcatcggcg cggcagacat tcgggatata tatcttccca tgggcttttc
    13261 ccatttcaag atcgagggcc gcaccctcgg cagcgcagtg ctgctggagt ttctgctgca
    13321 ctacctgaca agacccgaat accatctccc ggtccgcgag gaaatctatc ttgacggcat
    13381 gctcgatctc ttttaaaaag aagcggagaa cgcggagcgc ggcgggggat cgaccgggct
    13441 gctttcccga aattttacaa agaattatga cgagggaaag gtgtgtaaat ttcttgtaaa
    13501 aaatgttctt gcacttttta cgatgttgta ctataatcac gacaaaaacc cggcatgttg
    13561 tctccggttt tgttactttt atccgaaacg ggtgtgaggt atggcatctt tcaacgctgt
    13621 tatagagcat ctgcacgaag tgaatcttgt ctccatgctg gtgcgctttg ccatcgcact
    13681 tctcggcggc ggcatcatcg gcatgtcccg cggcaagcgc cagcacgccg ccggactgcg
    13741 cacgcatctt cttgtgtgca tcggcgcggc gtccgttatg atgacgagtt tgtatcaggc
    13801 acagaatctc ggtgtgccgg gcgatgcgtt ccgtatgccg gcgcaggtcg tgagcggcgt
    13861 cggcttcctc ggcgcgggca gcatcctcgt caccggcaag cgccacgtca ccggccttac
    13921 gaccgccgcg ggcctgtggg cctcggcgtg catgggcctt gccgcgggcg cgggcttcta
    13981 tgagggtgcg ctgatgatgt gtgtgctcat ctatgttgtg ctcgtttctc tcaacaagct
    14041 cgattcgacc catgtgaagg cgtcgcgggt gatgaacgta tatgtggagc tcaagagcgg
    14101 cgtgccgctc agcgcgctgc tgcgcgcggt caaaagcatg ggcattcaag tcaacgacat
    14161 acaggatatc ggctcgcgca gcgcggaaac gcacggatac attctggagc tggagatcat
    14221 gcggccggag ctgggtcatg tagaggcggc cgaacaggtt gcaaagatcg agcaggtcga
    14281 gttctgcgag gaaatgaagg cgtaaaacgg tcacccccac tccgttgaag ggacttgttt
    14341 gaggggctgc ggctcaccgc agcgcactcg ctccgcccgc acgcttgcga tctgataagt
    14401 atttttgccg acgccacatg tcgccggcaa aaatgttttt aacctaacct tatttgctcc
    14461 tgcgggagca aactctgcga gaaaacagac cggcggcttt ccgaaaagga gagccgccgg
    14521 tctgtataat ttataatatc cagaagagcg ccgcgccgag gaacgtgccg acggcgtagc
    14581 cgagcgcgcc gcagatgaga cccggcgcag tgagcgcgtc gcagcgcagc tgctttgtga
    14641 ccgccgggat aaacgccggg ccgtacagcc ctgccgtcat cgtgacgatg gcgcagtccg
    14701 cgccgatgcg gaaaatgcgg cagagcagaa gatgcagcag cgcggagcat acggtgatgg
    14761 aggcgagcag cgcgaccgtg tgcagcagat tcccgccgag cgtcccgaga tcgagcgagc
    14821 tggcaagcgc gaacgaaaaa acgagaatga gataatgtcc cgcggcggaa ttttccttca
    14881 cattccgcac cttcggcacg aacgagagcg caagcccgag cgtcgttccc gcgatcatca
    14941 ccgccgggac gagcggatcg gtgagtgcgc cgcccttgac cttccatatc gcaaagccga
    15001 tgaccgcgcc gacgagcacg catgcgagcg agagggcgag atttcgcacg atgccgcggt
    15061 ggaagccgtt ccattcgagc gcgtccacat tttccgcgcc gccgttctca cgcatgtaag
    15121 cgccctgctt tgcgtgccgg tcgagaactt tggcgcagag aggcttcgca agcgtaagga
    15181 tgaatatgta aaagaccgtg ccgacgatca tatcggagag attgccaatg gcgatcgtgt
    15241 cgcccggcac gccgagaatg acgccgatgg cgttgaagtt cggcgagccg ccggtataca
    15301 tgcccgcggc catcgcggca agctgccgtc cccacgggaa gccggccccg agcgtccgtc
    15361 ccaaaaccac aacaacggcg ctcacggcaa gcagcagaag cgcaaacgag atgagcaccg
    15421 gctttgcgag ccgccgcaca tcggcgagat ttgtgccgaa catcaatagc ggaatggcga
    15481 gcccgatgca tacatagctg ccgatctgcc cgacat
//
LOCUS       DAWAAE010000031        15441 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1728_length_15441_cov_3.402444, whole genome shotgun sequence.
ACCESSION   DAWAAE010000031 DAWAAE010000000
VERSION     DAWAAE010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15441)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_15441_cov_3.402444"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1538..1547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10331..10340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3192 a         4663 c         4285 g         3281 t
ORIGIN      
        1 cgcttgccgc gctctatctc gccgaccgga aacggttttg tccggcgggc gaatgcggag
       61 ccgctctcac gccgcggatg ctgcgcatcg cgctgccgct cgcttcgtct gcctatgcgc
      121 ggagcgcgct caacacgttc cgccagcttc tcgtgccgcg gggtcttcgt ctttcggggc
      181 tgagcagtga gagtgcgctc gcgggctacg gcgtcatcaa cggcatggcg atgccggttt
      241 tgcttttgcc gacgtgcctg ccgctctcgg tcgcggagct gctcgtcccg gcgctcacgg
      301 agatgcaggt cgccggagag cacgtgcggg tgcgccggag cgtgcgggag atgctcggga
      361 aaacgtttct tctctcgctc ggcgcggcgg ccgcattttt cgttttcgcc cgcccgctcg
      421 ggatgctgct ctacggcagc gcggaagccg cgcgctttct ccggctgctc gcgccgatgg
      481 tgccgttcct ctataccgac atcgtgaccg acggctgtct caagggtctg ggacagatga
      541 tggcgtccat ggcgttcaac attgccgagg ccgcgctggg gcttgttctc gtgtgggccc
      601 ttctcccccg ctgggcgctc acgggctatc ttgtgacgct ctacatctgt gaggttttca
      661 acttcacact gagccttctg cggctgcggc agattataaa cggggacagt tctcattgac
      721 gaccttgtgt cagagagaac cgtccccatg gataactttg gataacttta ttcctttttc
      781 gcatcggcga gattcacgcc gatgccgttt acctccacgc cgccgtcggc ggggatgagg
      841 atgtatttcc gcccgtcgat ctcgcgcgtc tcgacgagat acccaaactc gcccggcagc
      901 gtgatcttca cctccggcgt tacgatctcg agctttttgc tctcgacgat gttgttcggg
      961 tcgagcgcgg cgtgcgtacc gtagcgcttt tcacattcgc gctcgaattt ttccgtcttt
     1021 tcaagcggca caccgctcgt gcgcagcacg ctcaccacgt cgcgcgccgt gatctccagc
     1081 gcatccgggt ctttggcttc cttgtgctct tcaatgcggc tgcgcagatt ttcgtgcacg
     1141 gcctgcacga cgtcgaaccg gcagtcggta tcgagcgccg cggcgagcgt ttcggagaac
     1201 gtctccttct gctcggcggc ggacatcggc ggcgcgacac ggaacagccc gtcgatcagc
     1261 tcgggatgga tctccgcggt atcatggctg tagtagagcg cgtcataaat gttcgcgcgg
     1321 cggtcatcga acgccgggaa cagaaagcca agcgccgggg ccgcgacggt ctggccggtg
     1381 gacgtgccgt gaaaggcgcg ttcctccgaa aaataccgca gcgccgccgt cgcatccttc
     1441 acggggcaga ccgtgcagac gaagaagcgg aacacctcgt cgctcagctc gtccttctcg
     1501 ccgtccgcgc cgcggtgcgg cacgtcgtag gcgtcgcnnn nnnnnnnccc gcgaggagaa
     1561 tgaggtagtt ttcgccctgc atatcgagcg aggagatgac acgcgaataa agctcatgcc
     1621 gcgcgttctc gtcggcgcac gcgctctcgc gcagggcctg cagcagccgg tgctcgtcgc
     1681 tcccggcgac ctgcttcgtt tcaaacggga tatcgatgag atttcgcccg agcgagccgg
     1741 agagcgtttt gcgcaggagc gtcagataca tctcctgctc ctccttgccg gacatgccga
     1801 gagacacgtc cgtttccgcg acgacctcgc gcataccgtt tacaaagcag ccgtatacat
     1861 gggcaatgcc gctccgctcc ggccggaacc ggcggcggag ctcgttgagt tccttctgat
     1921 tcattatgta cctcttaaaa tgagaaaatg gcctgcgcac cgtaggtgac gatcgagacg
     1981 atcacgccgg cgatcacaac gccgatgacg atggccggga acgcgcgctt gagccgcatc
     2041 tccatcatcg ctgcgacgag cgcgcccgtc cacgcccccg tgccgggcag cgggatggcc
     2101 acgagaatgg cgagacccca gaacgcgtac ttttccacgg cggcgcggtt tttctccgct
     2161 ttcttctcca tgcgcgtcac gagcccgtcg agcttcggca tatgcgcgcg catccacgcg
     2221 aagatcctgc ggatgaaaag gatgatgaac ggcaccggca caagattgcc gagcaccgag
     2281 gcaatgatcg ccgtccagag gttcagcccg cgcacgacgc cgaacgggat cgcgccgcgc
     2341 agctccacga ccgggaccat cgctacgaga aacgtgagta aaatgtcttt catgggcatg
     2401 acgccgtccc ccctgtcgga acggcctgat cctccgtttt attacagttc tttcgccgcg
     2461 cggcgcgtct ttttccgccg ctgcacgaat gcgctgagtt tatccagcag cttgtacatc
     2521 acattcatca ccggccaggc aagcagcgcg aacaccgcaa gcacgagcgc gctgagaaaa
     2581 tcgagcgtca caaggttgaa aagcgccttg aggaacagaa agctccccgc aaagccgagc
     2641 gcgagcacgc agatcatcgc gatgcgcagc ttgttgtacg gtttcgccgt ctggtgcacc
     2701 atcatgagtc ccacaacgcc catcacgccg gcacagatcg tgctcatggc ggtgtccggc
     2761 agatcggcgg ccagatagaa gagcatcacg ccgccgacga gcaggatatc cgtgagcgcc
     2821 gccgggagcg cgcgccgcac cacgtttttg agaaatttgc cctgcacgag cgagcggttc
     2881 ggctccatgg cgaggatgaa cgccggaacg ccgatcgtca tgcagttcac aaggccgagc
     2941 tgcgcggggc tgagcgggta cggcagagtg aagatcagac tgatgatcgc catcgcgatc
     3001 gagaacatgt tcttcacaaa ataaagcgag gcggagcgct cgatgttgtt gatgacgcgc
     3061 cgcccctcag cgacgacgtc cgggagcgag gcaaagttcg agtctgtgag cacgatctgc
     3121 gatacctgac aggcaacatc gcttcccgag gccacggcga tggagcagtc ggcttccttg
     3181 agcgcgagca cgtcgtttac gccgtcgccg gtcatggcga ccgtgtgccc ctgttttttg
     3241 agcgcaagga cgagccgccg cttcatctcc ggcgtcacgc ggccgaaaac gacgcattcg
     3301 cgcgccgcct tttcgacctg cccgtcggtc tcgagcgtgc gggcgtccac cgcccggtcc
     3361 gcgtccggga tccccgcgcg gcgggcgacc tcggaaaccg tgagcgggtt gtcgccggag
     3421 atcacgcgca cggaaacgcc ctcgcgcgca aaccacgcga acgtatccgg cgcctcggcg
     3481 cggatcttgt tggagagcaa tatgagcgag atgggcaggc gctcggcggt gagtgcttca
     3541 tcggtgagct gcccgtcgta gagcgcgagg agcagcacgc ggcagccctt cgcgctgtag
     3601 tgatcgatct tttcccgata ggcgtcaaaa tcgccgccga ggatgacctc cggcgcgccg
     3661 agaaggtagg tctcatcctc atggaacgaa acgccgccgt attttttcgc gctcgtgaac
     3721 gggagcgcgg cgatcgcgcg ctgattcacc tcgccggtga agaacttgcg catggcggcc
     3781 atggtgtcgt tatcgccgcg catcgccgcg cagtagtccg ccatgatcat gtggatatcc
     3841 tcggcgatga accggtcagg gcagagcggg acgatatcgt ccacgatcat tttgttttcc
     3901 gtcaccgtgc cggttttgtc cacgcagagc gtatcgacgc gcgcgagtgt ttcgatgcag
     3961 tccatctcgc ggcacatcgt ctttttctgc gccagacgca gcacgcccgc cacaagcgca
     4021 agactcgtga gcagatacac gccctccggg atcatgccga cgagcgcgcc taccgtggat
     4081 gtgacgcccg cggggatgct ccggccgagc cagtggactt cctttaccac gagcagcacg
     4141 cccatcggga tgatgccgat gccgatccat ttgacgaggt cgttcagcgc cttcatcata
     4201 cccttggcct gacgcttttt ctgcgctttc gcgtcgagcg tcaaacggct gacgaacgaa
     4261 tccgcgccca cggcggtgag ccgcgcgcgg cacatgccgg agacaagaaa gctgcccgag
     4321 agcagcgtat cgcccggcgc tttgcggatc tcgtccgcct cgccggtgac gagcgcctcg
     4381 ttgacaaggc acgtcccgtc caccacgacg gcatcggcgc agacctgctc gccggtcgaa
     4441 aagaggacga tatcgtcccg cacgagcgag gacacgtcca cgcgcttttc gcgcccctcg
     4501 cgcacaacgg cgcattgcgg cgcggtcaat atattcagcg catcgagcgc ctttttgctg
     4561 cgcagctcct gcacgatgcc gatcacggcg ttaataatga cgacgatgac gaacgaaagg
     4621 ttcaaaatcg acgccttcac cgccacaagg cacagcgcga gcacaagaaa cacgaggtta
     4681 aagtatgtga gaagattctc ccgcacgatc tgccccgtgg tgcgcgtggg cggttcaacc
     4741 gggcgattgt cccagcccgc ggcggcgcgc gcggcggcct cggcctcggt gagcccctcg
     4801 aagggcgcgg cctccttcac ggcgatctcc tgccggacgg cttcctcttc tgtttttctg
     4861 cggaacagct tcaaagcgcc aagccctccc ccgaaaaaag cgttatgcat tagtatacca
     4921 ttttttcgct ctttttcaat aaagaccgtc ttaaaattgc gtaaagaacg cctgccttcg
     4981 gatgacaaaa gcaggcgttc gttcatttta tcgttttact cgatgatcat gcggcgggca
     5041 cgcataagcg taaggcggtt ccgcacaagg ggcgagcagc gcaggagctt atccagatcc
     5101 gcagacgaaa caccgatatc ctccggcgtt cgttttgcgt ccagccgttc cagaacacga
     5161 aaaagctcct ccggagcagg gatcgcggcg ataatctccc gaagctgccg ccagctttcg
     5221 cccagtcgtt ccggcgtgac ctccgcaaga caatcgcatc ggttttcctc ctcgatagac
     5281 cggcaaagcc gcgcaccgaa aaagacccgc atttcttccg tcgggaacgg ggtatagcgt
     5341 tggacggcgg gtgcagcgtc cccaatttcg gcaagccgct tatattctct cgccgccagc
     5401 agcgttccga cgcccgcctt ttcgccgtgc agcgccggaa aggcaaccga aagttccgcc
     5461 ggatgcattt caatgagatg tgctatgtgg tgctccgcgc cggacgccgg gcgggaattg
     5521 cccatcatct gcatggcgat cccgctcaac aagagcgcct ttgtcacgtt ctcaaacgct
     5581 tccggctcgc cgcggagaat gccgtccacg ctttccaccg cagcgtcagc cgcagtatgc
     5641 atcaagtcgt ggatctcggg gcagaaatat tcccctgtca tcacatgcgc catccgccag
     5701 tcggcaagcg cggtatattt agcgagaata tcgccgacac cgctgcgcac cagctcgatg
     5761 ggcgctgcac ggaggacgtc ggtatcggcc gcaacaattt ccggcgctac ggcgggcaat
     5821 gtctttttat atccgtgcca cgtcatagcg gccaccgtgc tgcaaaagcc gtctacactt
     5881 gctgccgtag ggcaggaaac aaaacggagc ccgcgctcgt gcgcgcagta gcgcgcgata
     5941 tcatggatcg tgccgctgcc gacggcgatc agcaccttgg tatctccgtg aagctcggca
     6001 agcgtgcgtg ccacggcttt ttcgtcggcg tgaaggtctt tggaagggag gatgatctcc
     6061 tgctccgcat acggatggcg tcccgctgtt gctgcatagg tccgctcgcc gtaaagcgta
     6121 cagcgtttcc cctctatgcc gttttcgtgc agcgcatctt ccagcgcaag aagtacgccg
     6181 ggcgcgacaa gtaccgaacg cgtcaccatc gtgtgatctt tgccgcagcg gcataatccg
     6241 ccaaattgcc gcgcatccaa tgagatcatg tgtttttctc catttttagc gtttccactg
     6301 cgagccgttc gagcgggaag gcggctttat agcgctcaag gaacgatgtg aagcctgcgg
     6361 catccacgct gtcgggcaat accgtagaga ccccggcatc ggcaaagacc cggttatcca
     6421 gatactcttc caataattca cccttcctgc gccagagcat atatgcgcag agcagtgcca
     6481 tgccgtaagg tccgccctca cctgccgttt ccattacgga taccggtgcg ccgaccgcgg
     6541 cggcaaggat cctctgcccc accccgggcg ttttaaagaa gccgccgtgg gcgcatatgc
     6601 gctctacatg gacattctct tcgcgccgca gaatgtccag tccgatgcgg agcgtggaca
     6661 gtgccgatag aaggtgcatc cggcagaagt tcgccagtgt aaaggcgctc tccggggtac
     6721 ggacgaagag cggtcttcct tcgttcacgg cagtcacgcc ctcgccggaa aaatagttat
     6781 agctcaaaag tccgccggcg tcggcatcac cctccagcgc cttgcggaag aagatggtat
     6841 acagctcgcc gcggtcaatg ggaagtccgg cagcttcggc aagctcggaa aagaggctga
     6901 cccagccgtt gatatccgat gtgcagttgc tgcaatgcac catcgccacg ggaagccccg
     6961 tcggtgtcgt caccatatcc acctcacggt gaacgccgag agggtgatct gccacaagca
     7021 tggcaaaggc gcttgtcccg gcggagacat tgcccgtaca cgcccgaaca gaatttgtgg
     7081 cggtcatgcc ggttcctgca tcgccctcac agggtgcgac ggggatcccc gcctgaagcg
     7141 ttcctgtcgg gtcaaggaat cgcgcacccg cctcggtcag atatccggcg atctcgcccg
     7201 ccgggacagg ctccgggaga atatcagcaa gacgccacgg atactttttc gtttccggca
     7261 gcgcttcaaa ggcttccgtc atctttgcgt catagccgag cgtttcgctg tcgagcggaa
     7321 aaatgccgga ggcttcgccg agtccgacct tcttctcgcc ggtcagccgc caatggacat
     7381 agcccgccag cgtcgtgaga tagccgatgc gtgccacatg ttcttcgccg ctgagcatgg
     7441 actgataaag atgcgcgatc gtccagcgct gcgggatatg aaagcggaag agctccgtga
     7501 gcttctcgga cgcctcaccg gtaacggtgt tgcgccacgt gcgaaatggt gcgagcggca
     7561 cgccggtctt gtccatcggc agatatccgt gcatcatggc agatacgccg atggcaccga
     7621 atgtcgtcgg atcttccccg gttcttttcc ggatatcggc gcgcagatcg gcatagcagc
     7681 tctgcacgcc gccgacgata tcctccagag aatatgtcca gataccgttt tccagccggt
     7741 tttcccatac atggctgccg gaggcaacag gcgcatggtt ctcgtcgagc agaaccgctt
     7801 tgatgcgcgt tgtccccagc tcgatgccga gcaccgttcg ggaaaaattc agcaaaaagc
     7861 tcacctcagc ttccagacaa gatcttttat cagcagctcc tcctcgagct tttccggagt
     7921 gctgccgtcg gtgatatgga caaattcaat atccatgatg cgagcccagt cacgcagctg
     7981 ttcggcggtc acatcatacg agagtaccga atgatgcgct ccgcctgccg tgatccagca
     8041 cgcaagcccc gtggtcagat ccggctttgc cttccacata acgcgagcca ccggaagatt
     8101 cggcatcggc ataatgggct ttacggcctc gatgtcctgc acgatcaggc gaaaacgccc
     8161 gcccatatcg atgagcgtcg ccacgaccgc ctttcccggt tttccttcaa acacaagtct
     8221 tgccggcggc tcacggtcgc cgatgccgag agggtgtacc tcaatacgcg gtctctctgc
     8281 cgcaacgctg gggcagacct ccagcatatg cgcacccaga gaatactcct gcccctcaac
     8341 aagatggtag gtatagtcct ccataaaagc gctcgcgccg ttgccgtttt cgcccatggc
     8401 cttgaggatt gccgtaagtg cggagacctt ccagtcccct tctccgccgt agccgtagcc
     8461 ctgcgccatg agatgctgcg aggccagccc cggcagctgc cgcatgccat acagatcctg
     8521 gaacgtgttg gagaaagccg cgcatccctc gcggtccatc atgcgccgga tggcgatctc
     8581 ctcccgtgcc tgatagcgca cggtctcgat atcgtccgta gcgaaatcat aggaagcgcg
     8641 gtattcgtcc atgagcgcgt caatatccgg atccgtaacg gcgtccatct cgtgcacaag
     8701 ctcgcccacg ggccaggtat tgacctgcca gccgagcttg atctgtgctt cgaccttatc
     8761 gccctctgtg acggccacct cccgcatatt gtcgccgaag cgcatgacct tcatgttctt
     8821 cgataccgcc gcgccgaccg ccgccttcat ccaacttccc agccgcgcct gcaccggctc
     8881 gtcccgccag tatcccgcga tcaccttgcg cggcgtacgc agccgcgcgc cgatgaagcc
     8941 gtgctcgcgg tcgccgtgcg ccgcctgatt gagattcatg aaatccatgt cgatctcttc
     9001 gttcgggatc tcacgattgt actgcgtggc aaaatgacac catggcttct gcagcgaagc
     9061 gagaccattg atccacattt tcgacggaga gaacgtatga caccacgtga tgataccagc
     9121 gcattcgtcg tgatagttag cctctttgat cgcatcagcg atttccccag cgcttttggc
     9181 cgtcacttta tatacgacgg gatacggcag agctttggag agcgtctccg ccatttcctt
     9241 tgcgcgcgca tctactgttg caagcacctc cggcccatac aggcactggc tgccgacaat
     9301 aaaccagaat tcgtagtttt tcatatcatt tctgtctccc ttatgcatat tccttttaca
     9361 caaaaactgc gcatcgcaga aaaagggcat attcggcttc agggtctccc gagaaaggag
     9421 accctgttcc gtgtgtaaat cgagctaaag caaggaatct ttcataagtc tcattcgtat
     9481 tacagtttgc tgcatgaagc atcgtacact gactatcgat gggccactac cgccgtttcc
     9541 ggtcttttga cctctttagt gcagtaattc aaccgaagaa attggtaatc caagtctgag
     9601 gtacatagct gccgttcgtt ccatgcatgc agacaagcgt ccagccgaga atcaaactgc
     9661 ttacaaaagt accaagccag acattcttag tcagctggta agcttttcta ttcacgaaag
     9721 tcatgattgg gaggaacaga atcgcaccgt aagtgagcat gaaagaggag atgctctttc
     9781 atgggctgag agctgcatag tcaactataa tgctgatacc gcacagcagc cacacacccg
     9841 cgaccgccat tgcaaccgtt atgagcacgt caaccgccgg tttcttgccg gccaaggtga
     9901 catccgaaag atagttggtg gtaatgctgc tgatgaacat aaagggcagt gccaaaagag
     9961 catagtggaa aaccaactgc cactgatccg ccgtaagagt tgcaaagctt acctgccaga
    10021 tccgatagtc ctgctcggta agatactgca ccagctccaa tgttccgtat gctacagcaa
    10081 catatatcac agcagccaaa agcgtcttga gaacattcac gaagccaaca gcaatgttct
    10141 ttttagcaaa gttcaaaatg ctcttgtatc ccccggtaat gaggacgtaa ccgacggcaa
    10201 taaccaaacc aaagattgta agccaagaga ggaactgaat aggagaccag gcagtaatca
    10261 tcaacgggaa gaaactgctg gttttgaatg taaacggcgt tttcctcgcg ttagtcagat
    10321 aaatagccag nnnnnnnnnn cgtattgaga agcaccgtgc cgacacctgc gaaaatcgcg
    10381 ccaattttcc ggttcttttc cgggagcagc gtctcaccgg acacgcagat agtgctgtat
    10441 ttgggggtca tagtcagcag tttgactacc ggaatcagca gtgcgaacat ggcaagcagc
    10501 gctgcaaagt tcagaaactc tctccacgcg aaaaccatcc cgcccttgtc tgcatccgct
    10561 atggaaacat tgccatgaag agcctgattc atatagtcaa taacgagacc tgatgtctgg
    10621 ctagagaagg tttctttgga atgggtctcc ttggtgagaa gaacggtgcg cagggtacgg
    10681 ttctcaatgg cgtttgcaag tgcaggatcg gaagaaacgg aatcctcacg gaaggttcca
    10741 acgatggtgt tagcgcccgc attgtcgtcc aaacagtaat acgctccctg aaccacttca
    10801 tcgctgcttg gaatatacca ggcatctctg acatcgtcac tgctgttgaa tccgacgtaa
    10861 ccatagtcaa acatgccgtt gatgatgcac aggttaacct ggcagtttct ggtcacttcg
    10921 atgccggcga cttcaacctg ctgcttaggg ttgacatact gcgctgtgga accgatcaga
    10981 acagcagcac ggattctcgt gttataatat tctgtttcct gtgtcttcag aatttcatac
    11041 gctttgagcg catctgcgga cagggtctcc tcggcgatct cgtctgcatt gcgctcaata
    11101 tcgtcagcgg aaatttccac gccgaatcct tcatgaagta cattcagcag tctgtcgttc
    11161 agtgtgaagt agccgcagtc catcagcgca gcatatccag aacgtcggga cccctgagaa
    11221 tggcccataa cagcaatatt ctctttgtcc acaaattcca gtccgcgcat gaaattcact
    11281 gcatccaggc atcccatagg cgtgccgaaa atattgtatt ccagaattcc ctgatcgttt
    11341 tcattgtaga caggtgtttc gctggctccg ctgccgtaag cgttgatgga gaatacaaca
    11401 tagcctctat gcgccagttc ttcggcatag caacgaagca tctgatagat attgccggca
    11461 ccgggaacaa tgaccacacc gggataagaa tcttcgtcgg ttgttccgac aggatagtac
    11521 aattcgccct ctagcgctgc gccgcgggca tcaatggtga tcttttcaac tttaatacga
    11581 cctccgtcgg tcgtgagaag ctgggcacaa agactgaata aaacgatcaa tgcaataagc
    11641 gtcaaagcaa ggcgtttgca tccctttgcg gaattcaggt tgaaaaactt cttcatctcg
    11701 ttttcctcct cattatttaa ttatttgtcc ggcttagaat tgctgccctc ctttcttttt
    11761 agtcatacct aaagcctgcc gtacagcacg ttttagttgc ggacgtttca tctgccatac
    11821 gtcagaaatt tgcggtttga aaagcaacgt caacaattca cataccaagc tgtcattgcc
    11881 ctaaaaagcg acaccgttct ctgttttcaa gtatataaga atacaaaagt gtgcatttgt
    11941 caagacaagc aaaccacaat catcgtttct tacgagtcat ttgtattttt tatgtataat
    12001 taatgtgctt atttgtatcg tgatttttct ttctattgaa tttatttaat ttatgctgta
    12061 ttataaaaat acaaatgcag tcaagtaaag gaacaaattt atatgaaaca tcccgttaaa
    12121 caaaccgccg taatgactca cattatggag gacattcaaa gcggccgata cgccgacggt
    12181 agccgcatgc cgacggaggc ggagtactcc gagcttttcc gcgtcagccg tcagaccgtg
    12241 cgcaaggcgc tcgctgccct cgaagcacaa ggcgtgatcc gccgtgttca aggcagcgga
    12301 tcctatgtga accatccgtc acagcgccgc agaaagaccg ggcagatcgc cgttatctgc
    12361 acctatatca gtgaatacat ctttccatcc attctgcgcg ggatcgagca ggtcacaaag
    12421 gaaaacgact atcagcttct catgaattcc actaacaaca gcattgcctc agagcgtgcc
    12481 attctctcca agctccggga taacccgccc gacgggatca tcgtggaggg aacgaaaacc
    12541 gcgctgccga atccgaaccg ggaattctat cattcgctgg cagagaacgg agtgccgatc
    12601 gttttcatca acagctacta cccggacatt gtcggcgata acgtgatcca catcgtggcg
    12661 gatgattatt ccggcggctt taccgccacg caggcgctga tccgcaacgg tcaccggaac
    12721 atctgcggta ttttcaaaag cgatgacaca cagggcacca accgatattc tggatacatg
    12781 gacgcgatcg tacggatggg cgcatccgtg cgggataata acgtcatctg gttttccacg
    12841 gaaaccaaag agcttttgct ccgcagcatc ggtgattttc ttatgggtat tgacagatcc
    12901 tgcaccgccg tcgtctgcta caacgatcag atcgccgcta cagtgcttcg gttctttgaa
    12961 accaacgggg actatgccgt ccgcgccgtt gcaagctttg accgtgcgct ccccatcgac
    13021 agcaatatac tctcctttgt ctcctatccg cacccaaagg aaaagatggg ccgtgtcgcc
    13081 gcagagaagc tcttccgcat gatcgcagga tgccgggaag aatcccgcac gctcgcttgg
    13141 gaataagaag aaaagaggaa cggtttcgcc gcggcgaaac cgttccttta attatgctgt
    13201 tcgtttatcc cagcagccgc acgcgcgtta cgccggggat ggcgcgcatc tcctccgccg
    13261 ccgcttcgtc cagatgctcg gtcgtatcga agatcgtata ggcgatctcg ccgcgcgcct
    13321 tgttgagcat gtgctcaacg ttgacgttgc ccgcggcgat gatctcgaga aaccggttga
    13381 gcatgccggg ccggttgcgg tggatgaggc acaggcggct ctttccgagc cggtcgagcg
    13441 tcgcgttcgg gagattgacg ctgtttcgga tgttgccgtt gcggagatag tccacgatct
    13501 cgcgcgcggc catgaccgcg cacttttcct ccgcctccgg cgtcgtggcg ccgagatgcg
    13561 ggaagcacac gacatttttc tttccgacga gcttcgcgtt cgggaagtcg gtcacatagc
    13621 gcgcgacctg cccgctctca atggcggcaa ggagcgcgtc gtcgtccacg agctcgccgc
    13681 gggcaaagtt caaaatgcgc atgccgggct tcatcttcgc aaacgtttcg gcgttcaggt
    13741 agtggaacgt ctccggtgtg gagtggatgt gcatggtgag atagtcgcac gtgcggaaga
    13801 gaacgttgag atccgtgacg tgctctacct tgctcgacag gttccacgcg gaatcgaccg
    13861 agagatacgg atcgtatccg ctcacctcca tatcgagctt gagcgcggcg ttcgccacac
    13921 gcgagccgat cgcgccgagg ccgatgacgc cgaggcgctt gccggacacc tccggcccgg
    13981 cgaaggcgtt cttccccttt tccacgagcg cagggatctt atcgccctcc ggggcgaggg
    14041 attccaccca ctcgaccgcg ccgacaatgt cgcgcgaggc catggtgagc gcgcagagca
    14101 gcagctcctt gaccgaatcg gcgttcgcgc cgggggtgtt gaacaccgcg atgccctccg
    14161 cagtgcagcg ctcaatgggg atgttgtcca cgccgatacc ggcgcggccg atgcacagaa
    14221 gctccttatt gaatacggcg tcgtgcagct tggccgagcg gatgagaagc gcgtcgggcg
    14281 cgctcatctc gggactcacg gcgcaatttt ccgctttcag ctcgtcaagc cccttttccg
    14341 caatggcgtt catggttttt atattaaaca tggtgtttcg cctcaaattc cttcatagtc
    14401 tccaccagac gaaggacgcc ctcctccggc atggcgttat aaagcgacgc gcgcatcccg
    14461 cccgcaacgc ggtggccctt gagattgcgc agcccccgct cgagcgagaa ctttacgaac
    14521 tcggcgtccg taccggcgtc tcccgtgcgg aacgttacgt tcatgtcgct gcggctcgcg
    14581 cgctcggcac agccacggaa gagcgcgctc tcgtccagat agtcatagag gagcttcgcc
    14641 ttggcgctct tgcgcttttc catgcccgca acgccgccct gcttctcgag ccagtcgagc
    14701 acgagaccga gcatataaat attgtagcac gggggcgtat tgtacatgga gtccttgtcg
    14761 atcatgcgct gatagctcat catcagcggc gtaacggcgc gctcgtgccc ggcgagcgcg
    14821 cggtcgatga tcacgaccgt cagccccgcg ggggccatgt tcttctgcgc cccggcatag
    14881 acgatgccga atttcgacac gtccaccggg tgcgagagga tctccgagga catatcgcat
    14941 acgatcggca cgccgccggt ctccggcaca tacttccacg ccgtaccgaa gatcgtgttg
    15001 ttggcgcagt aataaaaata ctttgcctcc ggcgagagag aaagctcctc ctgcgccggg
    15061 atgcggtcaa agccggtagc ctcgctcgat gccgcgatgt tcacgcggca gtattttccc
    15121 gcttccttcg cggcgatatt ggcaaagttg ccggtcacgg cgtaatcggc cgtgtcgccc
    15181 tcggcggtca gattgagcgg gatcatcgaa aactggagcg acgcgccgcc ctgcaaaaac
    15241 aggatctcgt gcgtgtccgg cacggagagc gcctttttga gcttcgcttt tgtcgcggca
    15301 aatatatcca taaattcccg gctgcggtga ctcatctcca tcacggagag accgctgccg
    15361 tgatagttca gcagctcggc tgccgccgtt tgcagcacct ccgtcggtag caaggacggc
    15421 ccggcggaga aattgaatac g
//
LOCUS       DAWAAE010000032        15419 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1732_length_15419_cov_3.331229, whole genome shotgun sequence.
ACCESSION   DAWAAE010000032 DAWAAE010000000
VERSION     DAWAAE010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_15419_cov_3.331229"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4936..4945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2886 a         4802 c         4673 g         3048 t
ORIGIN      
        1 ccgacggcaa ggccaccaag atcatcatcc cctccgagat ccagggcatc gccggtcttg
       61 caaagggcgt cgtcgaatcc gtaaaggacg actgagcatc ccccttcccc cgcgaacacc
      121 gccgcagcgc cggccatccg gcgctgcggt tttccccgcc aacagccagt ttttccggtt
      181 ttgttaccgt ttttccggcg gcggccaacg cagaacgatc ccctctgctc gccgttttgt
      241 aacttttgtt gccggatttt gcgtcataac ggctaattta aacggcttta ttccggtaat
      301 aactgtgctt gattcacaaa aagctccgtt tctgttgaca ggtgggatac aatgtgttac
      361 attttggtta caaattcgag gtgatttgaa ttggcatccg ctaagaatgc gctggtgtac
      421 aagggacacc cgctccgccg caaggacaac atcctctatt acggcagcat gtccgacaag
      481 tacatcatca tgatgcagat catggacacg aaaccggtca agccggttgc cggtgcggaa
      541 agcgccggtg agctgaaggt cgccacccgc gtgagcgtcc agctgcagct caccgacccg
      601 acggtcaaga gccgcgaccg catcgtgaag aaatccgaaa aaggcagcct gtacgccgcc
      661 atggacgtgg cgagcgtgtg gctcgaacga gcgctgacga aggactaagc tccgaaacag
      721 cgcaccagca ccaggaaatg aggtatgggg atcatgaaaa actgcaagaa actgctttcc
      781 gttatcgtta cgcttcttct ggcggcgtct ctgctcacga gcagcgcgtt tgccatttcc
      841 ggcgaagcgt tcatcgcaac gccgggcgaa gtcaacctgc gtgacagcgg cagcttcaac
      901 agcaatgtta tcgacgccgc cccgcagggc gcgaccgttt atgttctcgg cgacgccggc
      961 tacggctggt acaaggtgag ctacaacggc aagaccggct acatggcttc ctactacctg
     1021 ctcttcaacg aggtcccgtc cgcggagaac gtagattccg tcccggacaa ggctccggcg
     1081 caggagacca cgcaggagac cgtgcagcag cccgcgcagg aggttccttc gcagacctac
     1141 gttgcgcctc ccgcgaccgg cgacagcaac gccaccgttc tcggctccgg catctgcttc
     1201 cgcagcggcc cgagcaccta cagcagcgtg atcgaaacgc ttgagaccgg cacgcccatc
     1261 cgcgtgaacg gcgtgtgcgg ctcctggtat gaatgcgtat ataacggcag caccggctat
     1321 gtctacggcg actatgtcgt gctcaacggc acctccaaga cctacaccat ctccgccgcg
     1381 accgtgtccg gcaatgacgg cacgatctac tccgagccgc agaccccgga cactaccgaa
     1441 aacaccacca ccgaggtgac gccgatcgag cctcccgcgc aggagcccga aaccccggct
     1501 gagactccgg ccgagccgga aacgcccgcc gcgcccagct tctcgcacaa cgcttccgcc
     1561 gcgcaggcga tcgtcgatac cgcgatgcag tatctcggcg tgccttacgt ctggggcggc
     1621 acctctcccg atgtcggctt tgactgctcc ggtctcgtgt actactccta cggtcagcac
     1681 ggctactcgc tcaaccgcgt ggcgcagagc atgtactaca acggcgtgga cgtggatctc
     1741 aacgacctgc aggccggcga catcctgctc ttcggcagca gcgtgtacaa catctggcac
     1801 gccgcgctct acatcggcgg cggcaacttc atccacgcgc cttactccgg cgccgtcgtg
     1861 agcattcagt ctctcagcag caccgccggc atgcgtctcg tcgcggcccg ccgcatcgtg
     1921 taacagctta cccccgctta tcctcgcctt cgtctgacgc ccctcaaaag tggcgggacg
     1981 atgatccgga tacggcattt ttccccgaag acttcggtct tcggggattt ttgttctctc
     2041 ggaaacggcg cagcccccga aagtcaaggc tttcgggggc tgcactttct tttatttttg
     2101 tgcggcggtc gcgcgcaggc cctgcacctt gcagggaatg ccgttgtcgg cggcgagctt
     2161 ttcgagcgtt ccggtcaggc gcggcgcggc ggcgttgagg atctccgcgg ccatgcggtc
     2221 gcgcacgctg tcggaggcgc gcagcagaac gtttgccgcc atgccgccgg agaggagcgg
     2281 cgcgagcggg ctgccgttct gcttgagccg gtcggcgact ttgggaagga tctcctttac
     2341 caggacctcg taatcgatat cggaaagttc catttcgagc tttatcattg tttttcctcc
     2401 tgtgggatat ttcccggcta ttataccaca tgcttcgccg ttcgtaaatc gccgcgctct
     2461 tgacattccg gcgcgccgtc cgttatactt tcgcgggaaa ggaagtcgat gttatggcat
     2521 accgattgat ggccgccgat tatgatctca cgctcaccgg ctccgaccgg aaggtttcgg
     2581 cgcacacgcg cgaaacgatc cgcgccgtat atgccgccgg aaagttcgta acgctcgcct
     2641 ccggacggct cggcggcagc gccgaggtcg cgcgtgagct cttccccggc gatgtgccgc
     2701 tcattctcgg aaacggcgga ctggtgcagg gctgcgcgac gggcgaaacg ctttccgaga
     2761 ccatgatgga cgaaccgacc gcgctcacga tgctccgctg gtgccgccgg agggcgagcg
     2821 ccgccatcgt ttacggccgc agcggcattt ataccgaccg cgtgaacgct gcttcggaga
     2881 tgtacgcgca gcgctcctcg agcacgccgc tgccgctgcc ctcccccgaa gaggtcgcac
     2941 acgaggggat atacaagatc ctgctgctcg gtctgccgct gacggtccgg cgcgcggcga
     3001 aaaagctgca cagcgcgccg ccgcagccgg tggactgctt tgcgtccagc ccggaagcgc
     3061 tggagatcgt accgacgggc gtcaacaagg ccgcgggtct cgcacgtgtg gcggcgatcc
     3121 tcggcatacc gcgcgaggag accatcgcca tcggcgacgg ggaaaacgac atttccatgc
     3181 tccgctgggc ggggctcggc atcgccatgg cgaacgcgcc cgagaacgtg aaagccgcgg
     3241 cggacgtcat cgcgcccgcc tgtgacgagg acggcgctgc ctgggccatg gaaaagtatc
     3301 tgctttaacc tgccgctctc acctgcgggc ggggctctgc cccgcccgtt caatgtcccc
     3361 tttccggggg cattttattt ttctgttctt tttcgcaggg cgcggcgggt gcggcggatg
     3421 tggcgctcat agtcgccgct ctccaacagc tcggcaagca caagctgctc aaagagcggc
     3481 acggtgcacg aataaaaccc gatccgctcg gcgtactgcg ccgcgagccc ttccgagagc
     3541 accatatagc ccatgcgcag cgacggcgcg accgtctgcg agaacgtgtt caggtagatc
     3601 acgtttccgc tctcggcgag cgagaacacc gtttcctccg gcttggacgc gaccgtaaat
     3661 tccgaagaga aatcgtcctc aacgatatac cgccccgact cggaagccca gcggatatac
     3721 tcgcgccgct tggccgcgga cgcggtgaca ttactcggat agctgcggta gggcgtaata
     3781 tgcagcacgg aggcgcgcgt ggcggcgagc gcgtcgctgc ggatgccgtc cgcgccgagc
     3841 ggcagaagct cgagcgaagc gccgtgcgcg cggtaaacga gcgcgatctt ttcatacgac
     3901 ggatcctccg ccgcgtagat gcgcgccgcg ccgagaagct gcacgatcag tccgtagaga
     3961 tattccgcgc cggagccgac gaagatctgc tccggcttca cgcggatgcc gcggctgcgc
     4021 gcgagatagc gcgagatcgc ctgccgcagc gcatcgcagc cgttgttcgg gcttttttcc
     4081 agaagctgct cgccgtactc gctgatgacg cggcgcatca cccgcgagag caccgaaaac
     4141 ggaaaatccg ccctctcccc cgccggaaca gccgccggtc ttaccggcgc gctcggcaca
     4201 aagccgtccg tatcgtggaa ctgcacgtaa tgcccgctgc ggcggcgcgc ttcgagatac
     4261 ccttcgtccg tcagcagcgc gtaagcgtgc tctaccgtga cgacgctcag cccctcctcc
     4321 tcggccagct gccgcttgga cggcatccgc gcgccgaagg gataatcgcc gcggacgatc
     4381 tcgccgcgca ggcgctcata caggcgcaga tacgccgggc ggtgttcgtt gtcctgtttc
     4441 atctggtatt attattttct ccttctctgg atattttaat atatccagaa cattgtaagc
     4501 tatgggtaaa aaaaaaacaa ggggagatat atccatgaca acgaaaacga aacacggcac
     4561 gcgctggctc tgcacgacgg cgctgctgat ggcaatgaac atcattatga gcatgagcgt
     4621 gttcagcgtt cccgttccgg gcggacatct ctatctgaac gacgtgatca tctgctttgc
     4681 cgcgctgatt cttgacccgc tgggagcgtt ctgcgtcggc ggtgtcggcg cgttcctcgg
     4741 cgatttcttc ttttatccga cgccgatgtt cgtctcgctc gtcacgcacg gattgcaggc
     4801 ggtcgccatt tcgctctgcg cccaccggat gatgaaggag aagcccgtga tcgcgtccct
     4861 catcggcgtg ctgctcggcg cggtcatcat ggtcgtcggc tactcgctcg gccgcgcgtt
     4921 catctacgcg acgccnnnnn nnnnnatcag atcctgcagg cgctcgtcgg cgcggcgctc
     4981 ggcatggtgc tgcgctggaa agtaaagccg ctcgcaaagc tcgccgggaa gctcggcgag
     5041 acggtgggat aaaggttgtc ttttccttag cgatatggta aaatcattgt cactcagcaa
     5101 aggagtgata gctatgaaaa gaaaagcttt tgcggcgctc gccgccgttt gtctcgtgct
     5161 ctgcctgatc ctcgccggat gcggtaccgt gagcgaggag gattatcccc cgctggagaa
     5221 ggtcgatctc tcctccatga cactcggaac ggtcagcaac agcacgtttt cgacgcagta
     5281 tcccgccgat aagtggaccg gcagcgacgg cacttccccg ctcatcatct attataacga
     5341 tacgcaggga accggacagg ccgtcaacat caacgcgcag cagtccggca cctacagcgg
     5401 gaagttcacg gaaaagtaca tgaacaagct cacggaaagc attaccgaat cgttccccaa
     5461 cattgagatc ctcaatgccg agctccgctc gatcaacggc aagagcgtga tctacaccga
     5521 aacggtcacg cgcttcaacg acgagatgct cgaccgtctt ctcgagcagg gcgtcatcac
     5581 gcaggacgac gttgacgccg ccggaggccg cgatgcgatc ctctccatcc cgcccacgga
     5641 tcagatcacg ctctacgccg tgaccggcgc ttacctctac atttacaccg gcacgtatta
     5701 cgaggagagc cagaaggccg atgtgctcga ggctctgacc gttatggcgc agacgaccgc
     5761 agaagtgaaa taactcgccg ctctccatag aaaaacggag cagtcagaaa tgactgctcc
     5821 gtttttttgc tttcattcct ccgtccagtc ccggacctgc tttttgagcc agtttgtcca
     5881 ggcctcgacg ccctcgccgg tcttggcgga gatcgggaag atctccagac gggggttgcg
     5941 ccgcagcgcg tactcgcgca ccttgtccat gtcaaaatcg aaatacggca gcacatcgat
     6001 cttgttgatg atgagcgcgt cgcacacggt gaagatgagc gggtatttga gcggcttatc
     6061 gtgtccctcc ggcacggaga ggatcattgc gtttttcacc gcgcccgtat cgaactccgc
     6121 cgggcagacg agattgccga cgttttcgag caccgcgaga tcgacgtccg ccgtgccgag
     6181 ctcggtaagt ccctgctcgg tcattccggc atcgagatgg cacatgccgc cggtgtggag
     6241 ctggatgctc ttcacgcccg tgcgggcgac ggtctcggcg tctacggagg aatcgatgtc
     6301 cgcttccata acgccaatgc gaagatcgtc cttcaagcgc tcgatcgtgc gcagaagcgt
     6361 cgtcgtcttg cccgagccgg gcgaggacat gagattgaga aggaatgttt tttcctcctt
     6421 gagctttgcg cggacgcggt cggcctcgcg gtcgttgtcg gcaaaaacgc tctccttgat
     6481 ctctatgatg cgtacactgt ccatttcagc aagcctcgat ttctttgaga ttgatctcgt
     6541 tgccctgcag gagcacggtt tcctcgctgc cgcacagcgg gcagacgcgt ccctgcggga
     6601 cggtcggata tgttcgattg cagctctcgc aaagcgtcac ggcgggaatg gtctcgatcc
     6661 taagctccgc gcccttcaaa acctccgtct gcgcaacggc ccagttccag caggatgtga
     6721 gctcgtgcgg cacaacgccg gacacctcgc caagctcgag cgtgacggag gaaacgctcg
     6781 taagaacatt ttcctccgcg atgcgctcga cggtttttat catgtggaat acgatcccaa
     6841 gctcgtgcat gctcagccct ttttgccctt gagcttgatc ttcacggcgc gcttgagcat
     6901 gtacggcagg atggccttca tcttgtcctc gctgcgcacc atggtgctgc actcgggacg
     6961 ctcgcggtgg agacggatgg cgtcaaagcc gcagcgcgtc gtgcagatgc cgcagccgat
     7021 gcacttgttc ggatcgacga cgctcgcgcc gcagccgaga cagcgggcag tctcggccct
     7081 gacctgctcc tcggtgaagc atgcgcggcc gtcctgcatc gtgcggcgca gctcctcgtt
     7141 gtagccgggg cgctggcgcg gcgtgttgtc aaagctgttg atatcgagaa gaacgttttt
     7201 cttatcgagc tcgatgaact gccggcggtt gcggccgatg gtgaggctcg tgttcggctg
     7261 cacaaagcgg tgcagggaca cggcgccctc cttgccggcg gcgatggcgt cgatggcgaa
     7321 cttctggccg gtgtaggcgt cgccgccgac gaagatgtcg ggctcggcgg tctggtaggt
     7381 cagaggatcg gccttgacgg tgccgttggg gttgaattcg accttcgttc cggcgagcag
     7441 ctcgccccag gcggcgtgct ggccgatgca gtaaaggatc gtgtccgcct cggcggtctc
     7501 agtctgggcg tcgtcgaacg tgggagcgaa gcggccctcg gcatttttga cggacaggca
     7561 cttgcggaag tggatggcct tcgcgtggcc attctcggta tcaatcgcgg tctgccccca
     7621 accggcatgg atggagatgc cgtcacgttc gcactcggtg cggtcctctt cgcccatggg
     7681 catttcgtcg tagccctcca ggcagtagag catgacgctc tcggcgccgc agcgcacggc
     7741 ggtgcgggca acgtcggagg cgatgttgcc gccgccgatg acgacgacct tgccggtgag
     7801 cttcgtatcg ggcgcaagat tgaccttctt tgtaaagtcc acgccggaca taacgccctc
     7861 ggcgtcgtcg ccgggaacgc ggagacgtcc gccgtcctgc agaccgacgg caacatagaa
     7921 ccccttgtag ccctgcgcgc gcagctcggg gatggtgatg tccctgccga catccacacc
     7981 gcagcggaat tccacgccca tctcgcgcag aatgtcgatc tcggcctcca caacgtcctt
     8041 ttcgagacgg aagttcggaa tggcgttcat cagcatgccg ccgggacggg gagatttgtc
     8101 gaacacggtc gggcggtagc ccttctcggc gaggaagtac gcgcaggaca tgcccgccgg
     8161 ccccgcgccg atgatggcga tcttctcatc aaatccgccc tcgaccttcg ggatgacctt
     8221 ttcggggatg aagcgcgtgg cagcttcgag atcctgctgg gcaatgaagc gcttgacctc
     8281 gtcgatggcg accgcctgat caacggtgcc gcgggtgcag gcgtcctcgc agcggcggtt
     8341 gcagatgcgg ccgcatacgg cggggaatgg gttttcgcgc ttgatgagct ggagcgcttc
     8401 gcggtacttg ccctgcgcgg cgagcttcag atagccctgc acggcgatgt gcgccgggca
     8461 cgccgtcttg cacggggacg taccggtatc gtagcagttg atgcggttgt tgtcgcggta
     8521 gttgatgtcc cacttatccg ggccccaggc ggtctcgtcc ggcagctcgt ggcgcgggta
     8581 ctcgatgggg ccgtccttcg tgcagagctt ctggccgagc ttcaccgcgc ccgcgggaca
     8641 cgtctcaaca cagcggccgc acgcaacgca gttttcgctc tcgaccttcg caacgtaggc
     8701 gctgcgggag aggttcggcg tgttgaaaag ctggctcgtg cgcagcgcgt tgcagacgtt
     8761 cacgttgcag ttgcagatgg cgatgatctt ctcttcgccg tcgatgttcg tgatctcatg
     8821 gacaaagccg ttctcctcgg ccttgtgcag gatctcgagc acctcgtcat aggtgatgta
     8881 atggccgcgg tcggtctcga cgacgtagcg cgccatatcg cccacggcga tgcaccagtc
     8941 ctgcggatcg tcgccgcagc cctcgccgag cttggcgcgg gacatacggc aggaacaggg
     9001 cgaagcggcg taaacgccgt cgtacttttt cagccagtgg gagatgtgct cgatggagac
     9061 ggacttgttc tcggtctcga tcgccttttc cacggggatg acatgcatgc cgatgccggc
     9121 gcctccggga ggcaccatcg gcgtgacctt ctccagcggg aggaacgtca tgcgctcaaa
     9181 gaacgccgcg acctccgggt gctcgtcgat ctgcgactgg cgcatgttga cgaactctgc
     9241 gctgcccgga acgaactgcg gcagcgtcca gcgcttctcg tggttcgggt ttttcccgtc
     9301 cagattttcc cagttgtact cgacaaggcc gatgtacgcc atctcggtga gcagcttttc
     9361 gagctcttcc tcgcttttgc cggtgagcgc ggcgatctgc gctgtcgttt tcggcttgcg
     9421 caccttcatc ttgagcgcga catccgccat ttcgtccgta acaacgcccg cgagtcccca
     9481 atactcggga tcgttgcccg tgaccttacc gatgcggtcg gtgatcatcg cgccgagctt
     9541 gaggatcttc tctctcggct cgtccgtgcg ggggatcgta aaggaatatt tgctttcatc
     9601 cacaggcatt ccggacttgg tctgcttatc catgctgact cctccacatt ttatatctct
     9661 tttgttgagt tttgcactat atcgatagca aaattatatt acacatcggc gtgttcgtca
     9721 agtatgtttc ccggctggtt tctacacaaa atgagccgtt ttcggtaatg aaaacggctc
     9781 atttttctat attttactgt ataaaagcgt tctccacgcg gatggcggca gggtcaagcg
     9841 aaggatccat gacgcggacg atctcgtcaa aggcggcgct gcgctccgta acgcagtatt
     9901 cgaccgcgcc ggtgccgccg ctttcggcga aaaggggccg cagcgcttcg gcgagcgctt
     9961 cgccgacatc gatcatccgc acgccgggca gccgcttttc gagcgccggg cgatacacgg
    10021 ggtaatgcgt gcagcccatg atgagcgcgt cgatattctt gccgtcaaag gcctccatat
    10081 accgcgagca aaccgtctcc gcgacgatat cgtccggcgc gatgccgttt tccacgagcg
    10141 gcacaaaaag cgggcaggcg cgggagacga catgtacccc ggggtctatg gcgcggatgt
    10201 gcgtttcgta tgccccgctc ttgaccgtcg cctgcgtgcc gatgacgccg acggagcgcg
    10261 ttttcgtcat gctcgcagca agccgcgccg cgctttcgat cacgccgtag atcggcaggt
    10321 caaacttttc gcgcagcgcg tcgagcgagt tggagctcac cgtgccgcag gcgatgacga
    10381 tcgcctccac gtccttgcgg agcaggaagc gcacgtcgtc ctcggcatag tgcgtgatcg
    10441 tctcgaccga gcgcccgccg tagggtacgc gggcggtgtc gccgagatat accatatcca
    10501 gctgcgggaa tgcccgccgg agctggcgca gcaccgtaag accgccgatg ccgctgtcaa
    10561 aaacaccgat tcgatgcatt gttctcacct cattgaaacg attttacacc gaaacggtga
    10621 agaatacaac cggaaatcag cccgcagcat tcccggccgc gatctcctcg ccggtgacga
    10681 tgttgcgcac ccagatgccg cggcggagaa gcacgatgcc gatgaccatt ttgataaggt
    10741 cggcgagatt cacaaagagg tacgcctcca cgagcggcag acccgtttta tggatgagca
    10801 cccacgcgat cgataccgac acgacccagg tgaacacgct gtccgaaagc atggtgatgt
    10861 acgtccggcc tccggcgcgg atggtccagt acgcggcgtt taccacggcg tggaccggca
    10921 gcatgagagc gcagatgcgc atgagcgacg cggcgagcgt catgatctcg tgggaggtgt
    10981 tgtaaagccg cggggcatac ggcgcgacga ggaacatcac aacgccgacc gccgcgctgg
    11041 ccgcgcagct gagcgcggcg agcttcgggc agtaattccg cgcgcggtca tactcctccg
    11101 cgccgagcag attgccgatg atgatgccga cggtgttgcc catcgtgaag ataacggtat
    11161 tgaagagatt gagcaccgtg gagttgatgt tcagcgcggc aatggcgtcc aacccgcggt
    11221 aggagtagca ctgcgtgagc atcgtcatgc cgagcgacca gaacatctcg ttcacgagca
    11281 gcggcatgcc cttgcggacg atctgtccgg cgagatggcc ggggatcgca aagccgcggt
    11341 acagcccgcg gataaagcgc ggcggcgttt tctttccgtg ggtgccgagc aggatcaggc
    11401 cgaactccac atagcgcgag agcaccgtgg caatggctgc gccgcggacg ccgagcatcg
    11461 ggaagccgag cttgccatag atgagcagat agttgaaaac gagattgacg aagatcgccg
    11521 cgatgctcgc cttcatcggc aggcggttgt cgccggtgac gcgcagtatg ctcacatacg
    11581 ccgtcgtaag gcagaacgcc ggcagaccga agagcatgat acgaaggtag tcctcgcctt
    11641 ccgagagcgt gagcgcgaga tcgccgccgg tgcccgcctc gtgcaggaac gcgccgatga
    11701 gcgtatccgg gatgagcgcg agcagcaccg tggcgatcac tgtaaacccg gcgcagagca
    11761 ggatattgaa gcgcagcgta tgccggacgc cgttatagtc tccgcggccg aaaaactgcg
    11821 cgccgaaaat gcccgcgccg cccgtgccgc cgaagagaca gaggttatat acgaacagaa
    11881 gctggttgac gatggacacg cccgacatcg actccgtgcc gatccgcccg accataaggt
    11941 tatcgagcag attcacaaac gtcgatacgg cgttctgcag cagcagcggc agcgcgagcg
    12001 ccgcgaccag cttgtaaaag tctttgtttc cgataaattt cttcatagat cctccggcaa
    12061 aaagtgcggt gtctctttgc gagacaccgc attttttcat ttgttcatcg tccggaggag
    12121 gccctccggc tcaggtggtt ttcttgtggt ggtactgata agaggtgtcg atgcggttgc
    12181 aggcaacgaa catctcctcg tcgagctcgc gcttcatctg gagcgcttcc tcgatgtcca
    12241 tatcctcgaa acggccattc cgctcgacga tgacgcggct cgaggcatcc gacttgagca
    12301 cgtcgatcgc gcgcacgccc atcatcgcgg cggacacacg gtcacgtccg ctcggcgcgc
    12361 cgccgcgctg gatgtagccg aggaccgtca cgcgggtctc gagtccggtc tcctcttcga
    12421 tcgtctgcgc gtactcgtgg acatggccca cgccctcggc gacgacgacg atgtggtggc
    12481 ggcggccgtg gatgcagccg cggcggatca catcgatcac atcatgctcg agatcgactt
    12541 ccttttcggg aagcagcacg gcggaagcgc cggcagccaa ggcggtgtag agcgcgagat
    12601 ggccggcgtt gcggcccatg acctctacga ccgacacgcg ctcgtggctc tgcccggtgt
    12661 cgcgcagctt gtcgatggcc tcgatcgcgg tattgcaggc ggtatcaaag ccgatggtat
    12721 actcggtgca gcccatgtcg ttatcgatcg ttgcgggaat gcccacaacg cggacgccac
    12781 ggcgggccag ctccagcgcg ccgcggtaag tgccgtcgcc gccgatggtg ataaggccgt
    12841 cgatgccaag atatttgcag acttttgcgg ctttgtcaac accggcttcc gtgcggaatt
    12901 cctcgctgcg ggcggtatag agaatggtgc cgccgcggcg ggcaatgccg tccacgtcgt
    12961 tgccggtcag ctcaaaaacg tcgccgttga tgagtccggc atagccgcgg cggatgccga
    13021 tgcaggtgat cccctcggcg ccggcggtgc gcacaatggc gcggatcgcg gggttcatgc
    13081 cgggggcgtc gccgccgctc gtcaggatgc cgattctgtg tgctcctgcc ataaattact
    13141 tcaccctttc cgaatgtttt ttctttggtt gtaatatagc ccgattgatt cgattttgca
    13201 atagatttga cccaaaaaat cgtttccaaa ttgcaatttc agcgattttt gccaatgagc
    13261 gccttataga aatccaccgt gcccggcaaa cacgccgttt cgatgttctc gttgacgcca
    13321 tgcatgccgc gcatctgctc ggggccgtag atcaccgggg caaagcggat gcagttatcg
    13381 cagatcgcct gatagttctt cgcgtccgtc gcgccggtca tgacatacgg gctccaggca
    13441 aggccgggaa acacctcggc gatcgtatcg gtcacgcgcc gccacgcctc gccgtggata
    13501 tccgcggggg cagaataatc gctcgcggag agaacttctg tctcgatacc gtacttttcc
    13561 gcgcgctttt tgataagcgc gaggctctcg tccattccct ggtgcgggat gaaccggaga
    13621 ttggcccaca cgcttgcctc ctgcggcagc acgttgcagg cgtccgaacc ggagagcatc
    13681 gtgaaggccg ccgttgtgcg cagcatggcg cccgcctgcg gcgagagcgc gggcatgacg
    13741 agcttcataa gcggcgcgaa aagccagaga ttgccgaaga gaagccgcag agcgaagggc
    13801 gcgtacgccg cgagccgggc aaacatcgcg ctcacctccg gctcgaattt tttccggaag
    13861 ggcgggtgct tttccatcct gtccacaaac gccgcaagcc gcgccacggg cgagtttttc
    13921 ggcggcgcgc tcgcatggcc gccgttggag cgcgccgtga aacgcacatc cgctttcccc
    13981 ttttcaaaca cgccaagcat ggcgaaattc ccatgaatgc cgccgatggg ctcggagatg
    14041 atgccgccgc cctcgtcgca gacgaggaac ggcttcacgc cccgccgacg cagctcgtcc
    14101 acgagcttgc cgccgccggg tccggcgaac tcctccgtac aggaggacga gaggtacacg
    14161 tcctgctcgg ggacgtaccc ttcccgcagc agctcctcgc acgcctggaa aaagcccatg
    14221 acgctgcact ttgtgtctga agcgccgcgg ccccagacct tgccgtcggc gatgtcgccg
    14281 gagaacggcg cgtgctccca cgttccctcc gccgggacaa cgtcctgatg cgacatgagc
    14341 acgagcggcc tgtcgtggtt tttgcccttc cagaagaaca ggatattccc gtcgatctcc
    14401 gttttttcca gcttttcgtg tactgtcgga tacagctccg cgagcagctt atgaaagccg
    14461 cggaactttt cgagctccgg atcgtccggg tgcgacacgg tctcatacgc taccatgcgt
    14521 gagagctttt ccgcatactc ccgctcgcgc gcggcgtccg ggtgcggcgt ataatccgag
    14581 gtttttttct tcatgcacag tgtgcggatc actgccgcaa gcagaagcag cacgagcacg
    14641 ccgagcgcgg cgagcaaaac atagcctacg actctcatgc cgccgtctcc ttccgcgagc
    14701 ggcggtaccg gaagtacgcc acgccgatgg cgatcgcgcc ggagggcaat atcataaagc
    14761 tcgttgagcc gagcagcccc ggcacgagga tgaagagcac gctcataagg acaagcggcc
    14821 agagcgcgat cttcatgttc ttccggtagt acttatacgc catcgcgccg aagagcgcgg
    14881 gaacgacatt gtcaaatgcc ggctggagcg ccgggctttg cagcacgggc tgcagcggga
    14941 tgagcagcag cacgccgagc gcaagaatga cgatcgtcac gagcgacgcc gtggcgatcg
    15001 agagcgtgga gatgatctcg ttttccggcg tgccggtctt ggcccctacg atatcgcgcg
    15061 cgttgaccgc gcacgggatt ttcatgttga tgaggttgcc tgtgatgaat gcgagatacc
    15121 cgccgcccgc gccgagcatc ggcgtataga cgagatattc cgccacgcag ctcacaagcc
    15181 acacaatgcc gatggcaagg aagcccttcc cgaacgcgcg catatccggc atggcgccga
    15241 ggatgtatcc catcagaaac ggcgcgccga gcagcagcac gagcgttacg ccgcaggcga
    15301 tgcggccata ggtatgcgtc cggcggttgt aggcctcaaa ccggacggtc ttttcctgtt
    15361 cgttcattgc attccctcct tatacgccgc gcagcagcac cgctgccgcc atacccgcg
//
LOCUS       DAWAAE010000033        15139 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1767_length_15139_cov_3.841156, whole genome shotgun sequence.
ACCESSION   DAWAAE010000033 DAWAAE010000000
VERSION     DAWAAE010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1767_length_15139_cov_3.841156"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4040..4049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2907 a         4480 c         4819 g         2923 t
ORIGIN      
        1 tggatcttct tgtaggggcg gggctctgct ccgccctata aaatgatttc cgaaatatca
       61 gcaggtaata ccgtaaatgt gcggatgcat gtgcgaatac tgcggctcct cccaccagcg
      121 cgggccgccg tcccagtctt cgagaatgaa ccgctcgccc cagtcatcga tgatgatctg
      181 gttgccgttc tcatccacgc cgttcgtgcc gccgggcatg tagtacatct caaagaaatc
      241 gcgcatctcg tagtaatacg ggttgtaccg ccagatgccg ccggacggct cgcagccgtc
      301 atagaggaag agcgtcgtgt ccggcagcgc gttggcgagc tgccgccgct gcgcgccgga
      361 aagcgggatg ctcgggtacc agagaaggtc gagctgcgtc atctgcgaga gcgtgttcca
      421 gacattgtcc gcccgcacct tcatatagct cagattgagc gccttgagcg ccgtgcactc
      481 aagcagggga gagaggtcgg tcacgttcgt ctggaacatc tcaagatacc gaagctcctt
      541 gagctgcgcg agcggcgtaa tatccgtgat cgggttttcc gcgatgatga gatacgtcat
      601 gtgcggcata tactgcagaa agctcagatc agtgatgttc atgtggccga tgtcgagccc
      661 cttgagatcg gtgcagtatt tccagcagtc gatgtcgtcg ttcgtgagat agaccttgtt
      721 gtcgccccac gcggcggcga tgaaatactg cgcatcggtg cgcagataat agtttccgaa
      781 atagaccttc cagacgatgc ggatgttctc aagtttctga ttgagcgcgt caagctgttc
      841 gtaggagaga ttcgtgtcgc acatatcgac ctgcgtgagc ttcgggagct cggcaaacgc
      901 ggcctcgagc gcggacacgt cggcgatgga atagttgttg agatcgataa actcggtgtt
      961 ccactcgaac gtctgcccct cgaccgacac cgtccagccg gcggaaagat cttcgtgctg
     1021 ttccacaaag gcaagccggt cggctaccgg cgcggtgcag cccgtgagat cgaccgagcg
     1081 gagacgcggc agcagatcga gcgccgaggc gagctgcgcg agcgttgcgg cgtcctcgcc
     1141 ggcgagagat agctccgtgg tgatgttgga aacggtctgc gagccgatct gcacgttcca
     1201 gaaaaagctg atgttggggt aagccgagac gagcgagtgc tgctcgtcaa tggtaaagac
     1261 ggcgtccggc agattcacgg tgcgaacggc cggaaaatgc gcgagctgcg agagaagctc
     1321 catggccgtc ggcgcggtgc cggcagcggt gagctccacg gcgtcggaat cgaaccactg
     1381 cccgccgacg cagagactgt agcgcatttc gcagtccgga agctccgcgc ggagcgtttc
     1441 catcgcgtcc ggctgcgtac agtggttcac ggtgagcgag cggagatttt caaaaagccg
     1501 gagattctcc cagtcattcg gcagatcctc aagcgtgagc gactccgtgc cgctctcgtg
     1561 cggctcgtcg ttgagataca cggaatagag cacgcggcag ccggggtgct tggcgcggaa
     1621 ttcgtcgagc gcttcggcgg aaagcttgtt ctcacgcaca tccagctccg tgagcgcggt
     1681 gcacctgtcg agcggggcaa gatcggtgag ccccacgccg cgcagatcga gcacagctgc
     1741 gtttaccggg atgaactgct gcccgagccg gacataccgg cgcgagagga tgacccccgc
     1801 cgcggcggcg agcacgatga gcgccgccgc gcagatgacc agcacgcggg cgacgatttt
     1861 cgatttgtcg ttcttttttt cctgcatcgg aagtcctccg ggaatcaata accccagtat
     1921 tttttgtgga cggcggcgag ctcgtcgtcg gtcatgcgct ctacggtgtt gcccttgatg
     1981 tccatataat acatgtgaaa cgcgtcgcgc atgtcgtaat aatcctcgtc atagcgccag
     2041 gtgccgccgg tggattcgtc gccgggcgag caccagacct cggtgttcgg gagcgcctcc
     2101 cggagcgcct tgacctgcgc gtcgctcatg tacgttccgg tgcaccagag ccgccgcagc
     2161 cacgtcatct gcgagagcgt ttcaaaaagc gcgtctggcg gcgtgctgat gtagcatacg
     2221 ttcagatcgc gcagcgccgt gcagccgagc agcggcgtcg ggtcctgtac ggcggtgttg
     2281 aagagctcga gccattcgag ctccttgaga ttttcgagcg gcgaaatgtc gtagacaaga
     2341 tcgtcggcca gaatgagatg gcgcaggtgc ggcatgttcg ccacgaacgg caccgcgctc
     2401 agatggcagt ggccgagatc gagcgagcgg atgctgcgga gatagtacag cccgcgcggt
     2461 ccctgctgat ggacgttcat cttgaagccc gcgtcgggat tcgtgatgaa atagtccgag
     2521 tcggacggga cgaagaacac atagatgcgg agcgtccaca cgaacgttac atccggaaac
     2581 gcctcctgca gcgcgcccat ctcctcatcg gtgagaccgc agtcggtcat atcgacacgg
     2641 cgaacgcgcg gcagagcggg gagcgcttcg gatacctccg cgccggcatc ctcgatcttt
     2701 atgccgcgca ggtcgatctc ctcatcgtcc cagcggacgg tgacgccgta aagctcgacc
     2761 ggagcgggcg tcgcggtcgg gcgcggcgtc gccgtcggcg tcggtgcggg agacgccgtc
     2821 tcatttacta tgacggcggc gggggccgct tcgttcgcgc cgcacgcgca aagaatacaa
     2881 agcgcggctg cggcaaagaa tagtacaagg cttcgtttca ttttctcacc cggtgataat
     2941 gatctggttg ccgttttcgt ccacgccgtt cgtgccgccg gccatgtagt acatgccgaa
     3001 aaagtcgcgc atttcaaagt aatgcggatg ctcgcgccag gtgctgccgg tcggctcgcc
     3061 gtgcgcggcg agatacattt cggtgtccgg cagcgccgtt tgcagctctt cgagctgttc
     3121 ggcattgaga ttgttgccgc agtaccacag ccgttcgagc tgcggcatgt tgacaagcgt
     3181 ttcaaggcat ttggcggggc ggcagtagat ataggaaatg ttcaggtcgg tgagcgcctt
     3241 gcagttttgc agcggggaga tatcctcgca ctttgtccag aacatttcaa ggtaggtgag
     3301 ctcctgcagc tcgccgatgg gcgtgatgtc gttcacgtcg ttttcgacga tgatgagata
     3361 cttgagatgc gggacgtagt ggagaaagtc caggctcgtg atgtttttat ggccgagatc
     3421 aagcgcctgc aggtcggggc agtatttgag cacttccagt tcgtagctgt agatgggaaa
     3481 ctcgttgcgc acgcgcgcgg cgatgaagtt cgtatcgtcc gtgcgcaggg agaattcgga
     3541 aaagtatacg cgccagacga accggacatt ctcatgccgg tcgttgagcg cggccatgtc
     3601 ctcgttggat atgccgcagt cgcacagcac gaccttttca agcagcggga actcgccgag
     3661 cgcttcctca atgacggccg cgccggaaag ccccatgcgt atgccgctga agtccagctc
     3721 gagagcgtcc gtcgaaacgg tcttgccgaa aagctccagc tcgtatacgg taacggcgcc
     3781 gctcgcggag cggacggctt tgatctgctc cggcgtcagg ccgcagccgg tgaggtagat
     3841 gcgctcgaga gccgggaaac ggaaaaggtt atcgcagagc gtgtccacat cctgcgcggt
     3901 gagcgtctgc ccggcgaagg agagctcctt tgccgcagag gaagcggtca cgccgagcac
     3961 ttccacatcc cagcggaagg aaaggctcgg gaacgccgcg gtgagcgcgt cctgcatttc
     4021 ataggatacc gcgccgcccn nnnnnnnnnc cgtaaaggat accctgtaaa gctcgggaag
     4081 aaagggcagc gtttccagaa gctccgcaag cggcgtatcg gcgcgcagca cggcgcgctg
     4141 cgcgtccatc ggcacggcct tgccgccgac ggtcacgaac gcggtcagat caagcgccgg
     4201 ataggcgctt cgcagcgcga gcagcgcttc ataatccgtg cagccggacg catcgatgct
     4261 tttcaaattc ggaaagaagg caaagagcgg gatgtcgtcc gccgagagcg aagagaggct
     4321 cagactttcc gcgctgctcg gacggctgcc gccggagagc ggcacatccc agagaacggt
     4381 cttaccgggg tacttcgcgg cgagcgcttc ggcttcctgc gcggagatgt cctcgccgcg
     4441 aagatcgatc acgtccgcct ttcgggcgat cagccgcccg tcggagacgc tgtacgagca
     4501 gaaaaaccat acggcggcag ccgcgagcaa gagcacgagc gccgcgatga cccaccctat
     4561 gcgggttttg gaagatctca tctcttacag atcccacgga cggagcatat aggggttgaa
     4621 gcgccgctcg tattcgagcg aggtgctgcc ctcatgaccg ggatagacct cgtaatcgcc
     4681 gggcaggtcg cgcagctttt cgagcgagtg gccgagatcg agcgagctgc tgccggggaa
     4741 gtcgtaccgg ccgcaggaca tgcggaagag cgtatcgccg gtgaagaggc agtcctcgca
     4801 gcggtaggtg atgccgccgg gggtgtggcc gggcgtttcc agcacgtcaa acgtgagcga
     4861 gccggcctcg atcacgtcgc cctcgcggac aaacacggtg tcgtctggcg gatcgagctc
     4921 aaaatcttcg ttgccgaaca tgccgttgcc gatgtcggtc gtgagatcgg cgtgggacat
     4981 gtacaccgtt gcgccggtcg cgtcgtagac ctcctccagc cccatgcagt gatcgaaatg
     5041 gccgtgcgtg agcagaatgg cgcgcacatg gaaatggcag cgctcgatat agtcgagaat
     5101 gagattggag tccgcgccgg ggtcgatgac ggcggcgtcg agcgtcgcct cgtccgcgac
     5161 gatgtagcag ttggtctgca ggtatccgac ttcgatggct ttgatgagca tggttcattc
     5221 ctcctcggta atgggtgcgc cgcggcgcag cgtcacggcg tcgatctcct caccggtggc
     5281 ggcgctgtac gcgctcagag agatctcctc cggtgaaaag tcaaaaaata catagcccgg
     5341 ctcgtgctcg agcccttctg cggtgaacgg gaaatccgcg ctgcgggaat cgtcatagta
     5401 tttgagcccg gtgccggagg ggagcgtgat atagatccgc tcgcccggct gctcatagag
     5461 aatggaggcg gtatcggctt cgggcgcatc gccgccgagc agcgctgtgc ggttatattc
     5521 gtggtcgtgg ccggagatca cgagatcgac gcccagcgcg tccatcatcg gctcaagcgt
     5581 atcgcgaaag ccgatcaccg gcgcatcgtg cccatggacg ccgttgctgc gcagaccgta
     5641 gtggacaagc agaaccgtcc agttatgctc cggcgcgctc tctgcgacgg aggccataaa
     5701 atccgtatgg ttcgggaaat accggtcgcc gatctgcacg ccgatgaaca gcacgcccgc
     5761 gcgcacaaac cagtaattgc cggtacgagc gtcctgcccg gccatcggca gccgccgccg
     5821 cacggcgtac agcgaatcat ggttgccgga aacgggggcg agcagaaagc tccgcagcat
     5881 cggtacaccc tcgaaaaaca tatcgagcga gtactccgga tagtcgagaa tgtcgcccgt
     5941 gtggacgatc atgtcgatcg gctcgtgcgc ggcagtgctc ttttcgagcg cgctctcgag
     6001 cgtcgcttcc aggatcgccg gatggtattc gtgcgctcgc gcggaataga ggtgtgtgtc
     6061 cgaaaccgcg acggcgcgaa gccggccggg ctcgttcgtg cggaaatggt atacggcgtc
     6121 cggtgtcccg ccggccgtca cgaccgcata ggcataccgc gttcccggcg caacggcgag
     6181 ctccgccgta tactgcatcg cgtcggcgga aaagcagtca atgtcgagct ccgaagcctc
     6241 ggcggccgcg gcggcgggct cggacggcag cgagccgtcc gcttcggcgg ggccgtagag
     6301 aacgcacatg ccctcggcat gccgcgcgcc ggtccagcat acgcgcaccg ccgtgtcgtc
     6361 ctgcccggga agaagatagg gcgcggcgag agagacggcc gtttccggct gcagcaccgg
     6421 cacggcggtc ggctcgggcg tcggctccgg tgtgggagag agcgtggggg aggtcgtcgg
     6481 ttctatcgcc accggttcgg atgcggcagg ctccggcacg gaggaggaac agcccgaaag
     6541 aagcagcaca gcgaggagaa gcgcgagaag acagcgtttc attttcacag ctcccttgtg
     6601 tcgagcagaa tggtgacggg accgttgttg acggattcta caagcatctc cgcgccgaac
     6661 tcgccggtct ccacacggaa cccgctctcg cggcagagcg cgaccacgcg ctcatagagc
     6721 gggatggctg tctcgggccg cgccgcgacg gtgaagccgg ggcggcgcga ctgcgcgtcc
     6781 gcccagagcg tgaactggct cacaatgagg atctccgcgc ccgcgtccgc ggggcggatg
     6841 ttcatctttc cgttttcgtc gtcaaagacg cgcagaccgc agatgcggtc ggcaatcttc
     6901 gcggcttcct tttcggtatc gtccacatgg acgccgagca gcacgagaaa ccctttgccg
     6961 atctcgccga gcacttcgcc ggtttcggcg cgggttacgc gggcgctgtt gactcttgtt
     7021 acgacggctc tcatagatcc ttcctccgtt tatccgttgc cgcggtcgat ctcgcgcacg
     7081 ccgccgagcg tgcggatcct cgccatgata agccgcagcg aggcgagatc ggggacctcg
     7141 gtggtgacat agacaagcgc gttcccctcg gggagcgagc gggcgttgag cgagcgcacc
     7201 ttggcattga gcgcgttgag cacggcggca atatccagaa tgagcccgtt gcggtcgcgc
     7261 gcgagaatgc gcagcgttgt ggtgtaaagc cccaacgtct tatcggccca cgaaacattc
     7321 agccagcggc cttcctgttc gcgcttgcac agactgttga gatagtttgg gcagtcggcg
     7381 cggtggatgg aaacgccctg cccgcgcgtg atgaacccga cgatcggatc gcccggcacc
     7441 ggcgtgcagc agcgggagaa tttgatgagg cagttgtcca tgccctcaac gagaacgccc
     7501 tgcaccgcct tggtctgctg cgcgctgtgg cgctcgacgg cttcgttgat ccgttcaagc
     7561 tcgcttttct ggctttgcgc ctgcgcgcgg gcggcgcggg cgatctcgtc cttgaagcgg
     7621 ttgaccgcgc gtgtgcaggt gatgccgccg tagccgatgg cggcgtacat atcgtcgaga
     7681 ctcgtgacgg agagcttttt gatgcatgtc tgcaaaagat cgtcgtcctc gagatacgcc
     7741 gtcgtaagtc ccgcgcggcg cagctcactt tcaaacatct ccttgccgcg gacgatgttc
     7801 tcctcgcggc actccttttt gagccactgg cggatcttgg agcgggcggc gttggactta
     7861 cagagcgtca tccagtcgcg gctcggcccc ggcgcggatt tggacgtgat gatatcgacg
     7921 atatcgccgt tttgcagcgt atagtcaaac ggcacgatgc gcccgttgac ctttgcgccg
     7981 atcatccggt tgccgacggc ggaatggatg ccgtaggcaa agtcgatcgg gcacgcgccg
     8041 gcggggaggt tcaccacgtc gccctgcggc gtgaatacga acacctcgtc gttgaacata
     8101 tccattttga gattgtgaac gaagtcctgc gcgtcggtgt cctgctgcgc ttcgagcagg
     8161 cggcggatcc aggcgaactt ctcctcatct ccggcgcgga cgcctgcgcc gccggatttg
     8221 tacttccagt gcgcggcgat gccgtattcg ctgatgcggt gcatctccca ggtgcggatc
     8281 tgcacctcga acatgatgcc ctcctcgccg aggacggtcg tatgcacgct ctggtagccg
     8341 ttgggcttgg gcgtggcaat gtagtccttg aagcggcccg gcacggggcg gaacatgtcg
     8401 tggatgtggc cgagaacatt gtagcagtcg ggaatatcgt ccacgatgac gcggaacgca
     8461 cagagatcga agatctcgtc cagaccgcgc ttctgcgtat acatcttgcg gtaaatgctg
     8521 taggtatgct tgatgcggct ctgcaccgtt gcgtggatgc tgtactccgt gagccggtcg
     8581 gtgatcttct ttttgacatc ggacatgaac tcgttgagca cgtccgcctt gctgtcgagg
     8641 taggtgatga tctcacggta gccgtccggg tcgagatatt tgagcgagag gtcctcgagc
     8701 tcccacttca ccttctgcag gccgagccgg tgggcgatgg gcgcatagat ctccatcgtt
     8761 tcgagcgact tttcgagctg cttctgcgcg accatgtatt ccatcgtgcg catattgtgc
     8821 agcctgtcgg cgatcttgat gaggatgacg cggatgtcct tcgccatcgc catgagcatt
     8881 ttgcggaggt tttccatctg ctcgtcctcg cggctcgtga actgcacgcg ggtgagcttt
     8941 gtgacgccct ccacgatatc ggcgacctcg gcgccgaact gccgggcgat gtcctcgtgg
     9001 gtgatggcgg tgtcctcaat gcagtcgtgc agcagcgccg cgacgatgct gtcctcgtcc
     9061 agccccatct ccgcgcagat ctgcgcggcg gcgacgcagt gcgtcacata cggcgtgccg
     9121 gccttgcgct tctgcccgct gtgggcgcgg tcggccagct caaacgccgc gcgcacccgg
     9181 ccgagatcga cgccggggtt atgttccaaa atggtatcgg caagctcccg ataaccctgt
     9241 tcaatgattt cttctgccat gatagatcct ttttatcctt ctttgcttcg gcgcagatcg
     9301 gtgatcacaa gctgcacgct gcgccggccg tgaaattcgt tgatctgcgg cgcgaaaacg
     9361 atgtcgcagc gctgcccggc acgcgcgccg agcgcgggca cggtctggga aaagaacacg
     9421 ccgtcatagg tctgcccgaa tttggcgagc ttcagccgca ggtgcttccc gccgccgatg
     9481 ggctgcacgg agtcgagcga aacattttcc atatagagca gcggccgcgg gtttccgttg
     9541 ccgcacggct ccagctcgtc gagcgactcg acgcatgcca tcgtcaaaag ctccggtgaa
     9601 tcgacgagga ggtcggcctc cagcgccggg agcgtccggt cgggatggga gaggtaatac
     9661 tcacgcagcc gggagcgcag ctcatccacg cagccgcggc gcaccgtgac gcccgccgcc
     9721 atcgcgtgac cgccgaagcc ctccagacaa tccgagcacg ccgaaagcgc ctcaaagaga
     9781 ttgaaggagc cggtgctgcg gcaggagccc ttgccgctct cgccgtccag acagatcatc
     9841 accgcgggga cgctgtaggc gtccgtcagg cgcgaggcga cgatgccgat cacgccggga
     9901 tgccagcctt ccgaggcgag gacgatgggc gcgtccggct gctcgccgtg cagccgctcg
     9961 acggcctcgc gccagatctc cgtttcgagc gcctgccgct cgcggttttt ggcgcacagc
    10021 tccgcggcca gcacacgcgc gcggcgcaga tctgtctctt ccagcagacg caccgcgagg
    10081 ctcggctcgc cgagccggcc ggcggcgttg atgcgcggcg cgagcgagaa gcccacggtc
    10141 gaggcggtga acggcttatc gcgcacgccc gcctcctccg ccagcgcgag aaagccgggg
    10201 cggcagtcgc ccgcggcgat cttctgcaga ccgcagcgcg tgatgaagcg gttttcgccg
    10261 agcagcggca tcacgtccgc caccgtgccg accgcgacga gatcgccgta gcgcgcgagc
    10321 attttatccg cgtcgccgtc catggcgcac acgagcttga acgccacgcc gacgcccgcg
    10381 agcgccgtgc cgacgccgct gctgtccggc tgcttgggat caacgaccgc ctccgcctcc
    10441 ggaagcgctt cgcggcattc gtgatggtcg gtcacgatca tgtccacgcc gcgctgcgcg
    10501 gcgtaggccg tttcttccag attcgtaacg ccgcagtcca ccgtcacgat gagcgtgacg
    10561 ccctcttccg ccagcgcatc gatggcggcg gtgttgaggc cgtacccatc gcggatgcgg
    10621 tcggggatgt agagctcgca ggtgagaccg cgaaagcgca ggtaatcggt gagcaggcac
    10681 gcggcggtga tgccgtccac gtcgtagtcg ccgtatacgg caacgtgctc gcccgtcgcc
    10741 acggcgcggg tgagacgctg cacggcatcc accataccgg tcagctcaag cgggttcgtg
    10801 agcgtttccg cgccgccgcg gagaaacgag cgcgccgcgg cgggatcgga aagaccccgc
    10861 agatgcagga tcgctgcgag cagcggcgta cagccggcgg caagcagcgc gcgggggacc
    10921 tccgcgccgc gctccggcac attccagttt gcatacttca ttcggtttat atctccattt
    10981 tttaattaca aaaagagaag aaacagaaag gacattttat aacactttat tctaacaaat
    11041 gtccggccaa actgcaaggg agcggggcag atttttcctt gacacggcgg aaaatcaaag
    11101 ttacacttcc gatagatatg taatccataa agtaaaaagg agacaacgct atgcaatggc
    11161 tggaatgcac cgttcccggc ggggacgatc cggaggagct ctgcaacgcg ctcgccgagc
    11221 tcggcgtcgg cgggatgagc attgaaaacg aacgggattt ctacgatttt ctggaaaaca
    11281 accgcgcctg ctgggactat gtggacgaag cgctcgagga gagcttttcc ggcgtgtcgc
    11341 gcgtgaagtt ctggctctcg tgcgatgccg agggcgagaa agtgctcgct gcggtgcgcg
    11401 cggcgggcta cagcgtcgag acgcacgtca ttgccgacga ggactgggaa aacaactgga
    11461 agcagtatta taagcccctg cccatcgggg aaaagctcgt cgtcgtcccc gcgtgggagg
    11521 aacagcccgc ggacgggcgc gtggcgctgc ggctcgatcc gggcctcatc ttcggcaccg
    11581 gcagccacgc cacgacgcgc atgtgtctgg aggccattga atcgcttgcc gcgccggaca
    11641 agcgcgtgct cgatctcggc tgcggcagcg gcattctctc catcggcgcg ctcgtgctcg
    11701 gctgcaagga agccgtgggc tgcgacattg accccaaagc gccggacgtg gcgatggaca
    11761 acgccgcgct caacggcatc ggcgcggacc ggttccgtgt gtacgccggg gacatcctcg
    11821 cggacagggg gatgcgcgcc gcgctcggga cgaattttga cattgtgctc gcgaacatcg
    11881 ttgcggatgt tatcctgccg ctcgcaccgc tctcgcgcga atttctcgca cctggcggta
    11941 cgttcgtcac ctccggcatc attgagggac ggcaggacga gatcgagcac gcgctcaaaa
    12001 aggcgggcta ttccatcgaa aagcattttt gcgaagaaga atggcattgt tttgtgtgta
    12061 gcgtgcgcga ataacgggca tactacccgt acaaaatcgt acgggaggta aatgttccat
    12121 gatgatgaac ggcagaaaat ttatgacggg tctcggtatc ggcatggtgg cgggcggcgc
    12181 gctctgcatg atgatggcgc ccaaggccaa gcgcaacgac ggcaagcgta tggtgagccg
    12241 cgcgctcaag ggtatgggcg atgtgatcga cgatgtctcc gacgcgctgt gccgctgaga
    12301 gccaaaagca gcttttgacc gccgaaacga aaaattattt ttaatttttc aaaaaaaggg
    12361 gttgcttttt ccttccggct gtgctatttt aaatgagcgt cacagatatg cgccgttagc
    12421 tcagctggat agagtgtctg gctacggacc agaaggtcag gggttcgaat cccttacggc
    12481 gtgccaaaac aatccgatcg ccattcggcg gtcggatttt tttgcttttt tacttaattc
    12541 attctgcgct gcccagccgg tccatccagc gccgccgccg gaagaccgcc ggagacacgc
    12601 cgagctgtcc gattatgatc tgatcgacca tactgaacga cgatcgcagc atcgattgca
    12661 gtgccgcggg aaccgcgagc gcgcatacgg ttttaaagaa agcgctttgt cctttcataa
    12721 cgcacctccc gtttttcctc tttattgtaa attccgggaa ggagagaagc aaaggttaag
    12781 cgcataaccg aatacaaaat aaaagcaccg cgtttgtgca gaattatcct ctgcgcggcg
    12841 cggtgctgtt tcgtactacc agcgagacgg gcagcataat ctccgtctcg acggggcggc
    12901 cttctttgag ctcacgcagc ttctccacgg cgagcccggc gcgcagtgcg ccgtcctgcc
    12961 gcaccgtggt cagcgccggg cggagcattt cgcatatggg cgtatcgtca aattccgcga
    13021 cggaaatgtc ctccggtacg cggatgccct gctcggcgag aaagccgata agctcgacgg
    13081 cgtaggcgtc cgacacggca aaaaccgccg ctgcgcgccg gaacgtttcc agatgctgcc
    13141 ggtaaaaggc ccggcgctcc gccgtttctg ccgagacctt tcccgttttg ccgtggatca
    13201 cgttggaaac ggtcgctgtg ctcaatccca attcttccgc aatgtcgcag atgcgcacgc
    13261 gctcggccat aagatcgcct cctgccggta aacaagttta taattctatt gtatgggcgc
    13321 ggcagcgata agtcgaaggt tatccgcata accggcggcg aagtttccgg cggatacaga
    13381 ggaaatcctt tttatcggac ttccgttgtg gtattcttgc agcaaaggaa ggcggcagag
    13441 ccgccgcatc ggaaaggaaa gattttcatg atcgagatcc gcgggctgta caatacggcc
    13501 ctgtgctaca cgtctgcgct ggaagagaag gccgccgagc agatccgaac ggtctgcgat
    13561 caggaggagt tcgccggatg caggatctgc attatgccgg atgtccacgc cggaaagggc
    13621 tgtaccatcg gcacgacgat gacgatttcc gacaaggtcg tgccgggcat ggtcggcgtg
    13681 gacatcggct gcgggatgga gaccgtgcgc ctggccgagc gggagatcga ctttgccgcg
    13741 ctcgacgcgc tgatccgccg ggaggtcccc agcggcctga acgtccgcgg cggggagcac
    13801 ccgttcaacg cggagatcga cctttccgag cttcgctgcg cgcatagcgt ctcgctcgac
    13861 tgggcgcggc gcagcatcgg cacgctcggc ggcggcaacc acttcatcga gatcgaccgc
    13921 gccgagaacg gcgcgctgta tcttgttgtc cactccggca gccgctatct cggcacgcag
    13981 gtctgcgcgt actatcagga gcagggacag cttgccctgc gccgcggcgc gcaggagcgg
    14041 gtcaacgcgc tgatcgccga gtaccgcgcc gcgggacggc agcgggagat ccgctcggcg
    14101 ctcaaagagc tggacggcga gcgcgtgaaa cgcatcccca aagatctcgc ctatgtggaa
    14161 ggggaactgt ttgaggatta catccacgat atgcacatca cgcagcgctt tgccgcgctc
    14221 aaccgcaagg cgatcaccga cgtgatcctg cgcggcatgg gtctgacgaa ggtcgaggaa
    14281 ttcacgaccg tccacaacta catcgatacg gaaaagatga tcctccgcaa ggggtctgtt
    14341 tccgccgagg ccggagaaaa gctcctgatc ccgatcaata tgcgcgacgg cagtctgatc
    14401 tgcatcggca aaggcaacgg ggagtggaac tgctcggcgc cccatggagc cggacgcatc
    14461 atgagccgca cgcaggccgc cgcgcagctt tccatggacg aatacgccgc cgagatggaa
    14521 ggtgtctggt catcctgcgt ttcgccggag accatcgacg aatcccccat ggcgtataag
    14581 ccgttcgatg agatcgttgc cgagatcggc cccaccgccg agatcgcgga gcatatccgc
    14641 ccggtctaca atttcaaggc cgccgagtga gccgccgctg agcttgtcaa aaatactttt
    14701 ttagttgttt ttatttcaat aactgctatg tctttgtcca gcagtgcggt cagttcatca
    14761 gtagaaagtc cttcaatgtg acccagccgc gtatagctcc acgaattgga gccgaagaat
    14821 atgacaagct gattgccgga atataaaacg atatctcccg gctgcgtcgt tatctggaca
    14881 tcgtttcttg aaaagctctg cggcagactg ccaacctgct caaagctgcc atagcgcgaa
    14941 gcagaaatgt cttatttcaa atattcctca aagaacgctt tcagctttcc aaacggaatg
    15001 acgtccatct gatcgtaaag gtcagtgtgg tttgcaccgg ggataatgag caattccttg
    15061 ttatcgccgg tcagcttgct gtacgctgtc tcactgaaat agcgcgagtg cgccttttcc
    15121 ccgtgtacca gcagcacgg
//
LOCUS       DAWAAE010000034        14602 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1853_length_14602_cov_2.787448, whole genome shotgun sequence.
ACCESSION   DAWAAE010000034 DAWAAE010000000
VERSION     DAWAAE010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_14602_cov_2.787448"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4657..4666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5476..5485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2939 a         4399 c         4212 g         3032 t
ORIGIN      
        1 gcgcatacgc cttcgccgtt tccttttcca gctgcgcgag ccggtcgccc gcgtattcca
       61 gctcgtcgag acggcgctcg gaatccgcaa gaagcgcagc gagcccttct tcgtctgtgg
      121 aatatttctt ttccagacgc cgcagctggg cgagcctttc ctccagccgg tcatattcct
      181 ccggggaaaa gtccagaagc tgctgctttt cgcgcagccg ctcggaaacg tcctccagcg
      241 cgagccgcgc agagcggatg atctcttcgg tctccgcaag atcggcgctc cacgcgccgg
      301 cacgggaaac attatactcc gcatcgccgc actgtgcaac agcgctgctg tctgaatcgt
      361 agagcgcggc aagggcggcg gaaatgtgct ccgtcagctt ttcgctgttg cggagaagat
      421 cacgccgctc cgtgagttcc gcttcctcgc cggggcggat ctccgccttg gagagctcgg
      481 cgatccggta gcgcaggctc tcttccagac gctgtttttc cgcttcgtcc atggagagcc
      541 gttcgctctc gcgaacgagg gcgcggtagg catcatacgt ctcgcgataa gccgcgaacg
      601 cttcaccgca ctcgccgaag gcatccagat aatcgagatg ccgccgctcg tcgagaagct
      661 gacggccgtc gttctgcccg tggatatcga gcagaagcgc ccccagctcg cgcagctgcg
      721 acgccgtgac gggcacgccg ttcacacggc cggtgctctt gccgtccgtc gaaatgcggc
      781 gctgcaatat gatctcgccg tcgtccgcgt cgatctcgtt atcggcacac cagcactccg
      841 cctgcgccga ttcaaaggac gccgtgacgg acgcgcgctc cgcgccggag cggacaagct
      901 cgcggctgac gcggttgccg agtacagcgt tgagcgagtc gatgatgatc gatttgcctg
      961 cgcccgtttc gccggtcagt acgctcagtc cggcagaaaa ttccacatct gcgcgctcga
     1021 tcacggcgat attttcaatg tggagtgttt tgagcattac agcatagcct cgatttcccg
     1081 acagaagtcc agcgccgtac cgacatcgcg catggcaagg aacgcggtat cgtccccggc
     1141 gagcgtcccg acaagattcg gaacatccag attgtcgagc atagacgccg cggcgttggc
     1201 gaggccgggc agtgtgtgga tgacgacgat gttctgcgcc gtatcaaagc tcacgacaca
     1261 ttcgcggaag atcttgcgca gccggtcgag ctgatcgccg gaatcctccc gcgcagggac
     1321 ggtatagcgg tagccgccat ccggggaaag ctcctttacc agacgaagct cgcggatatc
     1381 gcgcgagagc gtcgcctgcg tgcttttcac gccgcgcttg cgcagcgctt cgatcaactg
     1441 gttctgggtc tcaatgttct gctcctgaat caatgcaaga atctcttcct gtcgctttga
     1501 tttcacgggg cgttcacctc tttcatttat cgactaattt ttcaaaaaca atgtcaaaga
     1561 agctgcgatc tttaacgcgc gccataagaa gcgttttatc ggagcgcgcc acgacgagct
     1621 cgtccccgtc aaggaacgga acggccacgc cgtccacgga aagaaagacc tctttgtctc
     1681 cctgctccac ggtgcggatg gatacttcgc ggtccgcttt tagtacatac gagcgcgccg
     1741 caaggcggaa cgtgcagatc ggcgtgagaa tgatgcagtc ggcgcacggc tcgacgatcg
     1801 gcccgcccgc cgccatggag taagcggtag acccggtcgg cgtggaggcg atcattccgt
     1861 cgccggaaaa cgccgtaact tctcttccgt cgctgaatgc ttcaagcttc acaacgctca
     1921 ccgaggcacg gacgaccgct tcgttgagag cgcttccctc atagaccagc tcgcccttac
     1981 ggaagagctt gagatcgagc atcatgcgcg tctgaaccgt gtaatggccg tcggccactt
     2041 ccagtatgca gtccgtctct gtctgctcca gttcggcgag aaagcccttg ttgccgagat
     2101 tgacgccgag gatcggaacg ccggaaccgg cgagataccg ggaaagctgc aaaatcgtac
     2161 cgtcgccgcc gagagagacg acgagcctcg ctccgggcag cgccatcgga agagcagcgg
     2221 tcgggatatc gcgcggccag gtgttttcca gcccgtcgct gaggaaggga gagatgcgta
     2281 cttcatgtcc gtgcttgcgc agcgcctcgg caatggcgag cgtggaagca aggccgtggt
     2341 cgcggtgggc gttcggacag agcagaacat atccgtcttt catttttcgg agtcctttcc
     2401 atgtgccgcg tgagacgcgg cgacgagcgc gtcgatgtcc ggctccggaa tgctctccgc
     2461 accctttttg agccaggcaa gatactctat atttccctcc gggccgcgga tgggtgagaa
     2521 atccagcccc cgcacgccga agccgcattc cggcgcatag cgcagaaatt cgcgcagcac
     2581 ctctgcgtga acggcagggt cacgcaccac gccctttttc cctaccttgt ccttgcccgc
     2641 ttcaaactgc ggcttgacaa ggcataccat ctcgccctcc ggcttcaaaa gcgcgccgac
     2701 cgcgcccaga atgagacgga gcgaaatgaa gctcagatcg gcaacggcaa cgtccatctc
     2761 ctccgggatc tgttcatgcg taacatagcg gatattcgtc cgctccatgc agacgacgtg
     2821 cggatcggtg cggaggctcc aggcaagctg accgtagccg acatcgacgg cataaatctt
     2881 ggccgcgccg ctttgcagca gaacgtccgt gaatccgccg gtggaagcgc cgcagtcgat
     2941 gcacacggcg tctttcacat cgatgggaaa cacacgcagc gccttttcga gcttccatcc
     3001 gccgcggctg acatacggca gcgtttcacc ccgcatctcc acagcggcgt cctccgctac
     3061 ggaggtgccg ggctttgtct ctttctggcc gttcacatag acgctgcccg ccatgatata
     3121 cgccttggcg cgctcgcggc tctcggcgag gcctttatca aatacaagct gatcaagccg
     3181 gatctttttc atgcagtatc tcctttgctg cggcggcaat gccatccgca tccagatgca
     3241 ggcagtgctg cagctcacgg accgaagcgt gcttaataac gccgctgccg agatccagca
     3301 gccgggcgcc gcgcagcgaa atgccgtttg ccgcggcgtc tgcaagaaga agcgtaccaa
     3361 gacttccgga acggcaggct tcctccgcca taataaaccg tccggttttc cgcagcgact
     3421 cacggacggt ccccggggcg agcggcagca gccggttgag cttgatgacc tcggcgcgga
     3481 tgccgtcggc ggaaagcgtc tccgctgcgg cgagcgctgc gttgatgctg atgccgtagg
     3541 tggcgatcgt tacatcgccg ccgctgcgca gaacggtcga atcctcgcgg gagtggtcgt
     3601 cggtatatgc gccctctccc ccacggggat accggagcgc tacggggccg gatttttcac
     3661 gcacggcgat ggatagcatg gagtccagtt cgcagaagct cgccggagca tatacggtca
     3721 tattcggcac ggagccgaga tacgcgatat caaaagagcc ctgatgcgtg acgccgtcgc
     3781 gcccgacaag ccccgcgcgg tccacggcaa gcacaacatg cagccccatg agcccgatgt
     3841 tctcaatgag catatcgtaa gagcgctgga gaaaggtgga gtaaacggca aaaatgggcg
     3901 tcagcccctg ctttgccatg ccggcgcaca tcgcggcggc atgctcctcg gcaatgccga
     3961 gttcaaaata ccggttcgga aaacggcggg caaattcgga cagccccgtg ccgtgttcca
     4021 tcgccgcggt aacggcgcag accgtcggat ccttttccgc aagtgcaatc gccgatgcgc
     4081 cgaatgccgc agaaaagtcg ttggtcggaa cggtcgtgac gccggtcttc cgatcgaacg
     4141 ggttgacgcc gtggtacgtc tgcgggtccg cctccgcggg ggcatagccc tttcccttca
     4201 ccgtcaccac atgcacaacg accgggcgat gaagatcctt cgcccagcgc agcgtagttt
     4261 cgagcgcctt ttcatcgtgg ccgtccaccg gcccgatata gtaaaatccg agatcgtcaa
     4321 atataccgga cggcagaagg cgcgctttga tgcgctcctt gagccggtgc agctcttcat
     4381 aggcgtgcgg gtgctttttc atcacacggc ggtatgtccg cttgaagcgg agatatccgg
     4441 ggcgcgtgcg tgcgcggcgg agcaatctcg ccacgccgcc gacattttcg ttgatggaca
     4501 ttccgttgtc gttgagaacg accacggcag cctcgcggct cgcaccgaga tcgctcagtc
     4561 cctcaaagca caggccgccg gtcatcgcgc cgtcgcccag gacggcgaca acgtcgtaat
     4621 ttccgtgcat gagcgttcgg gctcgtgcca tccccgnnnn nnnnnntcgg aggcgtgtcc
     4681 ggcaagaaac ggatcggcgt tgctctcgca cggcttggga aagccggaaa taccgtcgag
     4741 ctggcgcagc gtatcgaagg aatctctccg accggtcaaa attttgtgga cataggactg
     4801 atgcccgaca tcgaacagga tgcggtcctt catcgggtca tagacgcggt gcagcgccac
     4861 cgtcagctcc acactgccga gattcgaggc aagatgccca ccggtgtgcg acacgttttc
     4921 cacaagaaac tcgcggatgt cccggcatag ctccggaatg tcccgttccg gtacggcacg
     4981 gagatcggca ttgctgttta ttctatataa aagttctttt tcgttctggt tcaacacaac
     5041 tgcatcccct catactctta acttaagtat aataacaaaa acaaacaccc tttacaagag
     5101 caaagggtgt tttgacaaaa aatattcaca ttttatccgg aaaatattca cttcctgcgg
     5161 tccgccagaa ggtcggcgag ccagcagaga aattccgtat ccgcaaaatg tccggcagcg
     5221 gcggcttttg ccttctccgt atggagatgt acgagccggc cgcattcctc gacgccgaga
     5281 agcgaaacga acgttgtctt tttctctgca gcgtctgaac cgatggtctt gcccagctcg
     5341 tcctccgtag agattgtgtc gagcacatcg tcgcggatct ggaaagccag accgagattc
     5401 atcgcgtact cttccgcagc cgccacctgc tccggtgtac ctccggcgca ctgcacgccc
     5461 atgacgcacg cggcgnnnnn nnnnntcgta gagctccgcc tccgtgaccg gaagtccctc
     5521 tttgctcgtg tctagatatt gtccgccgca catgccctgc tcggcggcgg caaaggccag
     5581 cgttttggcc atcgccgcgg tacgctccgg cgcagccggc gaggacaaca cggcacagta
     5641 tgccgccgac tgcagcgcgt ctccggcgag aacggcattg gtctcgccga acatcttgtg
     5701 gcaggtgggc tttccgcggc gcagatcgtc gttatccatg cacggaagat cgtcgtggat
     5761 cagcgaatag gtatgcagca tttcgatacc gcaggccgca ggcagtgccg cggcgcggtc
     5821 tccgccggag atctcgcaga acttcacgag cagcagcggg cggatgcgct ttcctccggc
     5881 aaggagagaa tagcgcatgg cgtccagaag ctccttctgc gggcagtccg ccgtgaaata
     5941 cgtctccagc gcggcgttga tctcattctg gatggcttta tattcgctga aatgatccat
     6001 ggtcattcat tctccgatgc aaaggatgtt tcgaccggtg cgccgtccgc gcccttttgc
     6061 agcttcacaa cggtctgctc ggcctcgtcg agctgctttg tacaggtgcg gatcagctcc
     6121 gcgccctcgg tatacagcga aagagactcc tcaagcggag cgtcgccctt ttcgagcgag
     6181 cggacgatct cgtcgagacg ggcaagcatc tgctcaaagg tcttcggttt tgcggttttc
     6241 cgttcagcca tggtgatcct cctgtccgtt tcgatcagct ctttttatcg gtaacgcgga
     6301 cgatggtttc gcatttcacg tcgccaacgg tgcagatgac cttggtcgtt cccgccttca
     6361 cgccggtgac gacgccattt tcgttgacag tggcgatggc ggtatcctcg atcgtccatg
     6421 tctccttgtt cgtgcttgga tcgatgttgt acatcgtgca gttgagatcg accgattgtc
     6481 cgacagtaat ggtaaagtcg gaattcggca ggccgaagca ggtgatgacg acgcgcggcg
     6541 tcttatcgtt cgggtcaggc gtcacctgcg gcgcggcagc cgctgccgcg ggcggattat
     6601 cggccacgcg gatgatgcag accgctttgg ccgtgccgga cttgcagacg atgttcgtat
     6661 agcccatctt cttaccggtg acgagaccgg tctcggatac cgtcgcaatg gcggggtctt
     6721 ccgaggacca tgtgacctcg cttccctccg gcgtaacggc ggcggtgagc tggatctcat
     6781 atccccactg accgctgagg ctgatgtcgg tcttttccac atcgccgtag cagatcttga
     6841 tcgtcggctc cgtaacggtc agtgagagcg acgcggacgc atcgccgatg gtataggtga
     6901 tcttgggatt gccgaccttc cagcaggtga cgatgccgtt ttcgtccaca ctggcagagg
     6961 acgcgtcgct ggaggtccag atgccgccgg tggagccgtc gctcgcaaca gcctgcagac
     7021 ggatgaccgt ggaggtctgg aagctcagct ccgtcacggc cttgttttca tagaatatct
     7081 cgaccttgac cggctccttc gttgcctccg gtgttgcgga aacttccgga acaggcgtcg
     7141 ccgcttcggg cgttgctgtc gaagccgccg agggagacgc gttcgggtca tactgcgttt
     7201 tgttgtcctt gtttttgaaa aagtcggaga tgccgcagcc cgtcagagaa aagcaaagag
     7261 ccgctgctgc cagagcaagc gcaaccatgc gatatagctt tttcatcatt gtacctccga
     7321 ttgatgctcc acacggcagt caagcgcgcc gtgttcaagc cgtatatgga cgctgtcgcc
     7381 gggtgcgaca gccttggcgt ctctgaggat cgcgccggac gcgtcggtcg ccacggaaaa
     7441 accgcgcgcg agcaccttaa gtgggctcat cgcctcaagc gacgatacga gccggacata
     7501 ttcctgccgt ttggcgagca ggagatgctt ttccgccgcg ttcagacggt cggaaaggcg
     7561 atccacatcc agccgccggt tctgaagaaa gcccgtcggg ctctgcagca ccggccgtgc
     7621 cgaaagctcg ttcagccgcg aggcgagagc atccagccgc ttccgctccg cctgaccgag
     7681 ccggttttcc agcgagagga gaagcgcacg gacgtcggcc tgctccggta cggcgatctc
     7741 cgcaccgttt gacggcgttg ccgcgcgtac atcggcgaca aagtcggcga tggtcacatc
     7801 gggttcatgc ccaacggcgg agatgaccgg tatctcggac gcaaaaatcg cgcgggcaac
     7861 gcgctcgtcg ttgaacgccc agagatcctc gatggagccg ccgccgcggc cggtgatgat
     7921 aagatcggcc acattccacc ggttcgcgta ccggatcgca ccggcgatct ccgcgggggc
     7981 ttccgttccc tgcacgcgca ccggcatcac aaggatcttt gtgcgcggcc agcgcctgcc
     8041 gagaatacgg atcatatcct gtacagcggc gccggccccg gaggtgatga gcgcgatctt
     8101 ctccggcatg gcgggaagcg gttttttgtg ctcctgcgca aacagcccct ccgcgagcag
     8161 ctttgccttg agctgctcat acgccgcgtg aagatcgccc aagccttccg gctgaacggc
     8221 ggtgacatag agctgatagg ctccgtcccg cggatagacg gcgacacgcc cggagcagat
     8281 caccgccatg ccgttttccg ggcggaaacg cagccgcgaa gcgtcggcgc ggaacatgac
     8341 gcaccggatc gacgcgcccg catccttgag cgtgaaataa tgatggcccg agggatagat
     8401 cttataattg gaaagctcgc cgcggacata aacgcggcgc agcgcgccgt cgctttctac
     8461 gatggcgcgg atgtgctccg tgatctccga tacggagagc ggcgaaatct ccgtcattct
     8521 tccgtcgtag gtccgttctc cgccgctgcc gcgtcttccg cgggctccgt ctccgccggc
     8581 tgctcctcca cggtctcgcc gcgcgagcgc atgaagctgc cgaggatgcc gttgataaag
     8641 cggacggtat ccggctcatc gtaccccttg gccaactcga cggcctcgtt gatggacgcc
     8701 gccgccggga tatccggcat ataaaggatc tcataaagcg caacgcgcag cacggcgaga
     8761 gccgtacgcg aaatgcgcga gagcttccag ccgtgggaga actgctcgat gagatcgtcc
     8821 agctcctcgc ggtggtcgcg cacaccggtc acaagggcgt tgatgtaagc gcgctcctcc
     8881 tccccgggga tctccgtgaa aagagcatcc tccgccggaa gggcgcggaa atagtcctcg
     8941 tcaaaaaagg cgtccggtcc gacgtcgctg ccggcactaa cggcaaagct gagctgaagc
     9001 gccagctgcc gtgcattcgt tctcgtcata gagacttacc ccttcatatc ggtcttatca
     9061 aaggcaatgc ccgtgatatg cacgttgacg acggtcttga gtccggtcat gtcctccacg
     9121 ctcacggcga cctggtcctg caccttcttg gcaacgaccg tgacgccgta gccgtagcgg
     9181 accatgatga ggatgtcaat gacggcctgt ccgttctccg cggcgatctt cacactcggc
     9241 ggcgtggtcg cgctcttgat gccgaggaac tcggcgatgt ccgcgccggg cacggacgaa
     9301 agccctgcaa cgccttcgat ctccgaaaca gcggcgttga ccatcacctg gataacgtcc
     9361 tccgaaatgc tgatatttcc tttttccgtc tggctggtga tatagttttc aggcatagct
     9421 gctaacctcc caatgtattc atgtcattgt aacacaacga ttacaaaaaa taaagaggcg
     9481 tcccaaaaaa cttttggaac gcctcgctgc cgatatagtt gaagcggctc gaaatcaggc
     9541 acgcggatgc gatttctggt acacgtcctt gagctgcttt ttgacgagct gcgaatagac
     9601 ctgcgtggag ctgatatccg catggccgag catttcctgg atcgcacgga tatccgcgcc
     9661 gttttccagc agatgcgcag caaaagaatg gcgcagtgtg tgcggcgtga tgtccttctc
     9721 gatgtgcgcc ttggcctgat aggacttgat gatcttccag aagccctgcc gggacatgcg
     9781 ctcgccgctg acattcacaa agagagaggt ctcatccttt cgcgccagca tctgcgggcg
     9841 gacgaactcc atatactcgc tcaacgcccg gatcgccgcc ggatagagcg ggataaaccg
     9901 ttccttgtcg tgactgatgc agcggatcgc gccggctgtt agattcacgt ccgtcacatt
     9961 gagcgaaatg agctcgctca cacggatgcc ggtggcatac agcaattcca gcattgcctt
    10021 gtcgcggtag cccttgaagt cggtacactc cggctgctca agcagcagct cgacctcgct
    10081 gctcgtgagg atctgcggga gcttctgctc gcatttatcc ggaaccagct tgccggtcgg
    10141 gttttcgctc acgatgcgct ggagaatgag gaaggtataa aagcatttca gcgacgcgat
    10201 ggagcgggaa acggtcgcta ccgattttcc catcccgcgc agccaggaaa tgtatccgtt
    10261 caggacctcc tcgtccgcct cggtgtatcc gacagtttcg tgtgagtcaa ggtattctcc
    10321 gaactgacgg atatcgcgga gatacgaact cagggtgttg ctggaagcct ttttcacatt
    10381 cgtaaggtag tctttgaatt tttcaaagca tacggaatta tccatagcaa cgccctcctt
    10441 cctcttttct ttctctactg actatgctgt gctttatagt agtaacggga tcaaaagctg
    10501 ccggacgaat gccgcggcag acacgagcag cgcggcgatc agaagccggt ctgcggaaac
    10561 gaacgaaaac cggacggtcg gatagccgcg gcaggttcgg tatatatcca gagaggagca
    10621 gaacgcctcc tcgccaagca aaaacaaagc cgtaagagaa aagagagccg ggagccctac
    10681 gatgagacag gctcttcccg cggggacgcc gcccgcaagc agcaccgaca cgctatagga
    10741 aagaaggaac ccccgaaaaa gaaaaaggat cgggagaagc agaaagccga ataaaacggc
    10801 ggaaagaaac accgcgatga aacacggcgc ggaatatgtc cagaggagcc ggaggtatcc
    10861 ggccgagccc gaaagagagg cgtcggcaag cgacgcgacg aactccggaa ccggcgggaa
    10921 aatgcgtgat ccggcaagcg tcccgagcga cgcgccgaaa agcatcgcca gcggcaaaac
    10981 gcggcaggcc ggccgcgcaa ggcgtaaaat gcgcggcgct gagatcgccg cgtcatccgg
    11041 tgaaacgttc atgtcgtcac ctcgcctcgc ggcaaaagaa acaacataaa acaataattt
    11101 acataatctg ttgtacgcac actataccgc agattcataa agttatcaat ccctttttcc
    11161 gcgtttttag aattttgtga attatgtcca gattttatgt caactgtatt atgttatcct
    11221 gtgcaaccgg ataccgaaaa accattgaaa atcaaggatt tttcaccgta ttttgcgatt
    11281 acgctttgta atacccccaa gatgtcggtg aaacagttcc gcttatgtca actttgttat
    11341 ttgattccta tttgtaatag cccgcgcggc tttttcgtga ttttttgtta tttttgttca
    11401 tttttatgaa atacacgccc gtcatcatcg ccgtcataac gcacagcgtt ttcaaaacgc
    11461 aaccgatatc ccaaccgttc atcggaaggc aggctccgac aagaagcaaa atgagcgccg
    11521 cggccagcgc ggaaagcggg atatacgcat accctcccct tcgcttcccg aaacagaggc
    11581 actcggacaa tagcgccgca agaccagcgc atacaagcgc agtaagctgc accgacgttt
    11641 ccggcagcac tccctcgcgg atcagcagcg ccgccggaag cgcaagcaga agcgacgctc
    11701 ctgccataac gcttcccgac cgcacggcgt ccgcagcctg ccctttccgg ttcggctgtt
    11761 gtttgaccat aaaaacaccc ccgcagataa gtacattcat acttattcct gcgggggtgt
    11821 ttttatacgc ctgtatccgg tgcgatcagt tgcccagaga ctcctcggaa tcggaggtct
    11881 ccttgttggc gcccttgccc tcattgctgg agaccgccca cttggtgatc tcgatgcgga
    11941 cgcggtcttc accggtctcg aaggtgagct tgtcgccggt gacgtggacg accttgccga
    12001 ccatgccgcc gatggtgacg atcttgtcgc cgacttcgat gctgtcacgc atctcctgcg
    12061 ctttcttctt gcgcttcttt tcggggacga tcatcaggaa gtagaacaca gcgatgatga
    12121 cgaccatcat aacgatgctc gtaatgccgc tggcgccggt agcggttccg tccgccgcag
    12181 ccatattgca gccggcaaac gcgaacatgg caaaaactac gccaagaaca gttgcaagga
    12241 tttttttcat gggttaaccc tccaatgtga attacaagta ttgattatag cggtgttttc
    12301 cgataaaagc aagaaaatta tatccgctta tccaactttt cgacataccg gtcgtggaac
    12361 gcctgatact gcccctcgtc gagcgctgcg cggatctttt ccatcaggga attatagaaa
    12421 tagagattgt gcatcacgca gaagcgaagc gccaggatct cctccgcctt catcagatgg
    12481 cggagatacg cgcggctgta cttgcggcag acggggcatt ggcactcagg atcaatgggg
    12541 cgctcgtcgc gcgcgtactt ctcgtttttg atatttatga cgccttccca ggtatagaga
    12601 tgcccgtggc ggccgttgcg ggcgggcatc acgcagtcaa agaagtccac gccgcggtag
    12661 actgcctcga tgatatttcc cggcgttccg acgcccatga gatagcgcgg cttatcctgc
    12721 ggcatatatt cctcaaccgc agaaatggta tcgtacattt ccgcggcggt ctcccctacc
    12781 gcgagtccgc cgatggcata accggggaga tcgagctccg cgatctgctg catatggcgg
    12841 acgcggagat cgtggtacac tgccccctga ttgatgcccc agagcacctg tccgggattg
    12901 acggcgttgt cctccgaggt atagcgcgtg agagcctcct tgcagcgggc gagccagcgc
    12961 accgtgcggt cacacgacgc ttcgcagtac tcccgcggtg cgggaatgcc gacgcactcg
    13021 tcaaacgcca tggcgatatc cgagccgaga ttggcctgaa tgcgcatgct ctcctcgggt
    13081 cccatgaaga tatgccgacc gtccacatgg gaattgaagt atacgccctc ttccttgatc
    13141 ttgcgcagct gcgcaaggga aaagatctgg aatccgccgg agtcggtcag gatcggtccg
    13201 tcccagcgca tgaacttatg cagcccgccc atatcgcgga tcagctcgtc gcccgggcgg
    13261 acgtggagat ggtaggtgtt ggaaagctcg acctggcagc cgatctcgcg cagatcatcc
    13321 gcggagaggc cgcccttgat cgccgcctgc gtgccgacgt tcatgaatac cggcgtctgc
    13381 accgtgccgt gcggcgtggt atattcgccc cgtcgggcgt gtccttcgag cttgattctt
    13441 ttatacattt gtactcccct tatcgttaat ggataaacat cgcgtcgccg aaggagaaga
    13501 agcggtagcg ttcctttacc gcctcctcat aagcgcacag gacgttttcg cgcccggcca
    13561 gcgcggagac gagcatgatg agcgtactct ccggcaggtg gaaattggtc acaagcgcgt
    13621 caatggcctt gaagcggtag cccggataga tgaaaatgtc cgtccagccg cttttcggct
    13681 gcaatatgcc gtcttcgccg gtaaagctct cgagcgtccg gcaggaggtt gttccgacag
    13741 agatcactcg cccgccgttt gcgtgcgtgc ggtttacgat gtccgccgtt tcctgactca
    13801 ctatacagaa ctccgcgtgc atcgggtgat cctcgatatt gtcctccttg accggacgga
    13861 atgtgccgag tccgacgtgc agcgtaacat aggcgagcga aacgccttta tcggcgatgc
    13921 gctgcagaag ctccggcgta aagtgcagcc ccgccgtcgg cgccgccgcg gaaccggggt
    13981 cgcgggaata taccgtttgg taacgctcct tgtccgtcag ctcttccttg atatacggcg
    14041 gcagcggcat tttgccgagc ttgtccaata actcaagaaa gataccgtca tagttgaaat
    14101 gcagcacctt gttgccgtcc gcttccgcac ggatgacctc tgccgtcagc tctccgttgc
    14161 cgaaggagat atgcgttccg ggctgcgttt tgcgccccgg acgggtgagg cattcccagt
    14221 ccccgccgcc gagatcgcgc agcagcacga cctccacgct gccgcccgtc ggccgcgtac
    14281 cgaagagccg cgccgggatc acgcgggaat cgttcatcac aaggcagtcg ccctcgttga
    14341 gaaagtccgg gagctcgtgg aagacgtggt gctcggttgc ccccgtgcgc ttgtccagtg
    14401 tcagaaggcg cgaggcgtcg cgccgctcga gcggcgtctg ggcgataagc tccggaggga
    14461 gatcatagta aaagtccgat tttttcaaag ctgctccatc tgccggtatg tccggctgct
    14521 gtgttttgcc gccgtgctca ccttgacggg agtacggcgg agtgttatta tactttaaaa
    14581 tattatatac atgttccgct cc
//
LOCUS       DAWAAE010000035        14498 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1873_length_14498_cov_3.233054, whole genome shotgun sequence.
ACCESSION   DAWAAE010000035 DAWAAE010000000
VERSION     DAWAAE010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1873_length_14498_cov_3.233054"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2424..2433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5969..5978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2818 a         4473 c         4413 g         2774 t
ORIGIN      
        1 gtcaagcacc tgcacttcca tgtgctggcc ggccgcgaca tgacgtggcc tccgggctga
       61 gataaccaga aaagggacgg catacgccgt ccctttttgc ttgtctcccc ggggaaacta
      121 tgctacaata ccggtattcc gacccactgg atctatagca ggaggtacat atggagaaaa
      181 acggaacggt catggactat ctgcgctggc ggggcgatct gcccttctgc cgggacggct
      241 tcaacgaggt ggacaatctg gtgctgtgca tcatttccta tatcaatttc cacaggtttg
      301 acgatctgaa gaccaccgac cccgccaagg ctgtggcgct gccggaggta gccgcccgcc
      361 tgacggagga ggacgagcag cgcggcctgt cggagctggc gtatatcccg ctgatacgtc
      421 tggcggcgga gacggagcgc ttccgcgatg tgcggatgtt tggcttcacc cacgagtggg
      481 acgaggagaa ggagatgcag ttcgatgccg tatcctacct gctgccggac gatacgctgt
      541 tcgtggcttt catgggtact gacacctcgc tggtggggtg gaaggaggac ttcaacatga
      601 gcttcctctc cgctgtgccg gcgcaggagc gcgccgccgc ctacaccgtg gagatggccg
      661 ccgcctgccg gagatgcaaa ctgcggatcg gcggtcacag caagggcggc aatctggcgg
      721 cctgggccgc catccatatc ccggaaaacc tgcaaaagag acggctgctg gccgcctaca
      781 acaacgatgg ccccggcttc tcccacgata tggtggactc cgaggcgtac cgccgggtgg
      841 cggataagct ccacacctat gtgccggaat cctccatcgt gggcgtcctg ctggagcacg
      901 cggaggatta cgccgttatc gacagcagca accgctccat catgcagcat gagccgctgt
      961 catggaacgt gctgggagcg cggttcatcc gcttgggaga gcgctctcag atgggtaagc
     1021 tcagcgatga cgtgctgcgc cagtggatcg gctctatgac cccgcaggag cgggagcagt
     1081 tctccgacgc cctgttcgat gtgctgagcc tgtccggtaa ggcccggacg ctggacgacc
     1141 tgcgcgccgg cggactggcg ggcggcgcgg cgctgctgaa gcagtacaac ggcgcgaatg
     1201 aggaggacaa gaagatcatc tccgagatat tccgccgtct ggcggtggac gtgaaggcag
     1261 agctgaagaa ggctgccgga cagagcctga agacagcgga aaagagtctg gccgacatga
     1321 agcacgccat caccggcttc gcaaagggcg acaagaccga taaatgaccg gcagcgcccc
     1381 tccgtacaga cggaggggcg ctgtgatttt ccatggttga cggttacggc ggcgcgttgt
     1441 ataataaact gatttactat ctaaaacacg ggggtatggc ctttatgtgg atcgcaaagg
     1501 attggcagga ttacgagctg ctggactgcg gcggcggtga aaagctggag cgctgggata
     1561 agcagatatt ggtgcgcccc gacccgcagg ccatctggga gacggatcac cgcgaccggc
     1621 ggtggaagac cgccaacgcc cggtactccc gctcctccac cggcggcgga cactgggaca
     1681 aaagcaagct gccggagagc tggcccatcc actataagga actgacgttt caggtcaagc
     1741 ccatgaactt caagcacacc ggactgtttc cggagcaggc cgccaactgg gatttcgcca
     1801 tggagcagat ccgcacggcg gggcgtccca tccgggtgct gaacctgttc gcctacaccg
     1861 gcggcgctac ggtggcgtgc gccaaggcag gcgccagcgt ctgccatgta gacgcggcca
     1921 agggcatggt ggcttgggcc aaggacaacg cacggctcag cggccttgcc gatgcaccca
     1981 tccgctggat cgtggatgac tgcgccaaat ttgtggagcg cgagatccgc cggggcaaga
     2041 cgtatgacgc catcatcatg gacccgccca gctacggccg cggcccctcc ggggaggtct
     2101 ggaagctgga ggagaacctg taccccttcg tccggctgtg cgcgcaggtg ctgtcggaca
     2161 accccctgtt cgttatcctc aacagctaca cgacggggct tgccccctcg gtgctgggat
     2221 atatcctgca aatgctggta gggcgcagat tcggcggcca tgtgacgtgg gacgagctgg
     2281 ggctgccctg caccgattcc ggcatggcgc tgccctgcgg cgccacgggg cgctggtgcg
     2341 ccgactgacc aagaacggag atataacgca atgtcgacca tgatcgaaac aaaagacctg
     2401 accttcacct atcccgcgcc ggannnnnnn nnnctggccg ccgtaggtat ggagaaattc
     2461 gcctgccatg cgccccacct gctgtccggc ggacagaagc agcgcgtggc cattgccggt
     2521 gtcatcgcca tggagccgga gtgcatcgtg ctggatgagg ccaccgccat gctggatccc
     2581 gccggtcggc gggaggtgct ggacaccgtc caccgcctga accgggagat gggcatcacc
     2641 gtggtgctta tcacccacca tatgtcggag gcggaggacg ccgaccgcgt catcgtcatg
     2701 aacgacggcg tcgtggccat ggacggtacg ccccggcagg tctttgcgcg ggtggaggag
     2761 ctgcacggct tcggccttgc cgcgccggac acggtggagc ttttatacga actggggcag
     2821 gcgggtttgc ccgtaacgct ggacagcctg acggtggacc agtgcgccga taccatttgc
     2881 cgcgcattgg gcggggcgga agggaagtaa ccttggaacc gatcttacag atagagcatt
     2941 tgactcacac ctattcggcg ggtacgccgt ttcagcgcag cgccgtggag gatatgaacc
     3001 tgtccgtgat ggacggtgaa ttcctcggca tcatcggcca caccggctcc ggcaagtcca
     3061 ctctgatcca gcatctcaac ggactgctca agcccacatc gggccgcatc ctgttacagg
     3121 gcaaggatat ctgggcggag cccaagaaga tccgggacgt gcggtttcag gtgggactgg
     3181 tgttccagta cccggaatac cagctgtttg aggagacggt gtataaggac atcgccttcg
     3241 gcccgaagaa catgggactg gacgaggagg acatcgaccg ccgcgtccgt tccgccgccg
     3301 ccttcgtggg cctgacggag gccatgctgg acaagtcccc ctttgagctg tccggcgggc
     3361 agaagcgccg cgtggccatt gccggcgtta tcgccatgga gccgaaggtg ctggtgctgg
     3421 acgagcctac ggcgggtctt gacccccgcg gccgggacga tattctggca cgcatacagg
     3481 actatcacag ggcgaagcac gcctccgtgg tgctggtgag ccacagcatg gaggagatcg
     3541 cccgcaacgt ggaccgcatc gtggtgctgt ccgacagcca tgtgctcatg gagggcgcgc
     3601 cccgtcaggt gtttgcccgc gcccatgagc tggagcaggt gggactggat atcccgcagg
     3661 tgacgaaggt ggcgctggct ctgcggcagc gggggctgcc ggtggacacg gcggtctaca
     3721 ccgtggaggc gctgcgcgag gcgctgctcc ggctgaaagg gggcgctgac gtatgctgaa
     3781 ggatatcacg ctggggcagt atttccccgg cgacaccctc atccacaagc tggatccccg
     3841 gacgaagctg ctgtgcgtca ttttgtacat cgtggcgctg ttcaatgcaa aaggccccct
     3901 gacctacggc atcatgattg ccgtgctggc ggtgtgcatc ctcatctccc atgtggagtg
     3961 gaaggcgctg acaaggggct tgaagccggt ctatttcatc gtggccttca ccgccatcat
     4021 gaatatgttc tttaccaacg gtacgccggt ggcggacgtg tggctggtgc gccacatcac
     4081 gtgggagggc atcatcgccg ccgtgcagat ggtgctgcgc atcgtcatgc tcatcatggg
     4141 tacgttcctc atgacctaca ccaccagccc catcgccctg accgacggac tggagcatct
     4201 gctctccccc ctgaaaaagc tgcggttccc ggtgcatgag ctggccatga tgatgtccat
     4261 cgccctgcgc tttatcccca cgctcatcga ggagacggac aagatcatgt ccgcccagaa
     4321 ggcccgcggc gcggatttcg agtcgggcaa tatcttccag cgcgccaagg cgctggtacc
     4381 cattctggtg ccgctgttca tcagcgcgtt ccgccgcgcc gacgagctgg ccaccgccat
     4441 ggagtgccgc tgctaccacg gcggcgaggg gcgcacccgc atgaaggtgc tgcgcatggg
     4501 ccgaaacgac tacctggctc tcgctttctg ggcgctgatc ctggccgggt cgatcatcct
     4561 gacgaggtac ggcatttgag aaacattgca ctccgtctcc ggtacgacgg ctccgcctat
     4621 cacggctggc agcggcaggc cggtctcccc acggtacagc agaccatgga ggacgccatt
     4681 gcaaaaatac tcggtacaag cgtccatgta acgggctgcg gccgcacgga tgcgggcgtg
     4741 cacgccctgc gctactgcgc gaacttccgc tccgactgta cgatcccgat cgagaaggtc
     4801 ccgctcgcgc tcaacgcccg tctccccggc gatatcgccg tgacggacgc ctgcgaggtg
     4861 gacgagagct tcaatgccat cggctcgtgc atccagaaag aatatatcta taagatcctg
     4921 aacagccgca ttcccgatcc gtttctcgat aagcgcgtgt gcttctatcc gtcccggctc
     4981 gacattgcgc agatgcgcgc cgcggcgaag gcctttgaag gcacgcacga ctttgccgcc
     5041 gtgcggagcg tcggcaccga aacgaaaacg accgtccgca ccgtgtactg gtgcgatgcc
     5101 gaacgcgagg gcgatctcat caccgtgcgt gtgtgcgcca acgggttttt gtacaacatg
     5161 gtccgcgcca tggtcgggac gatggtatac gcaagctacg gaaagctccg ggcggaggac
     5221 attcccgcgc ttctcgaaac gcgcgaccgc cgtctcaccg gcccgacgat gccgccgcag
     5281 ggtctttata tgcaccgcat ctggtacgac ggccccgccg gggaaatgat ggcagacact
     5341 tgagcgccat tgacgcttga tttttcggag gttttgatgc attctaatag gtataccgcc
     5401 gcgccggagc gcaagcaccg gaatctgccc cgtgtgctcg ccgcgacagc cgccatgctc
     5461 tgcatcgcag cgctgttgct cgtgctgagc gtcgtccagt cgcacacggc ggccgccgtg
     5521 cggaaccgcg acggcttccg gctccggagc ggagagaatg tcttttattc cgccgccgga
     5581 aacgggcttg ccgccgccac gacgaccggc gcgcagctct tcaacgccag cgggaaatgc
     5641 gccgcgagcg ccgactttgc aatgacggag ccgatgtgcg ccgccgggac gcagctcgcg
     5701 gtgttctggg acgccgggca aaacggcatc cacgcgctgt atccggacgg ttcggccgcc
     5761 gagagcgcga ccgaaggcgg cgtatacttt gccgatgtga acgaaaccgg gcttattacc
     5821 gtcctaaccg ataaggacgg gtatcgcggg agcgtcgtcg tatacgacac cgatctcacg
     5881 ccgctcttcc gctgggatgc ttcgagcgtc acgcccgtct ccgcgcggac gaccggaaaa
     5941 ggccttctct gcgtgaacgg cgccggagnn nnnnnnnntg gatttcggct ttctctcgga
     6001 cggctccatt gccgcggtga cggaaacggc ggtttgcttt ctctccgcgg acggcacgct
     6061 gctctccgag caccggctcg gaaacgcgca cctctccgcc ttttcgctca gcggagactt
     6121 tgccgccgtt gcggcggcga gcgggctcgg cggcggcgag acggtgatca cagcgttctc
     6181 cccctccggc gagccgctgg ggagctttac cgcccctctg gaagccgcgg ccatgtcgtc
     6241 ctgcggcagc cagcttctcg tcctcttcac cggagaggaa tccacgctct tttcctccgc
     6301 gctggaggag atcgtatcct atcagccgcc ggaggacgtc cggcaggtgt ttctctgctc
     6361 ggacagccgc gctcttttcg ccggtgcgtc cggcgttgta caaatcgatt tcaaccggtg
     6421 atctctcacc ggccgcgcct gaaaaaggag gctttgcatg aaagcggtac tgagtctttt
     6481 gtttttgtcc atactcattt tctgtgtctg gtccggctat aagaaggggc tcgtcatggg
     6541 catcttcagt ctgatcgcgt ttgttgtttc ggtatacggc gcgaatcttc tctcccagac
     6601 ctattccgga gaggtgatcg acgcgctgcg cccgttcgcc agcggctttg ttgaggtgaa
     6661 cgtcgtcgac aaaaccgtcc gccccgccat ggggatcgac agcaccaacc tttccgtcaa
     6721 ggactatttt gcgcagaatc ccggccgtga aacggaattc tgcacgctca cctacaacgg
     6781 catgggcatt tacgaatcca ccagcgagca gatggcggac gaagccgtcg cctacgcgaa
     6841 ggaaaataac gctgagctca tcgacgccgt tgtagaagtg ctctgccgcc gcatatccta
     6901 tgtcggcggg tttattctcg cattcgttat gatcctgatc ctcctcacgg tgatcgggaa
     6961 cattcccaat atcagcttcc agataccgaa ccacgagctg ctcaacgaca tcggcggcgc
     7021 ggtggccggt ctggtgcagg gcatggcgtt ctgcctcatc atcggctggg cgctgcggtt
     7081 cgtaggactt ctcgtctcgc aggagacgct ttccaacacc ttcctcgtct cctggtttat
     7141 ggatcgcacg atcttcgtac acctgctcgg aatttaacgc catttcacag aatacaggag
     7201 aacatctatg caacctactc tctgcgcccg ctgccacaag aacatggcgg tggtattcat
     7261 caaccgtatt gaaaacggcc agtcccacca ggagggactg tgtctcaagt gtgcccgtga
     7321 gctgcacatc aagcccatcg acgatatcat ttccaaaatg ggcatcaacg acgaagatct
     7381 cgacgggctt tcctccgaaa tgatggaggc catgcagaat ctctccggca gcggggacga
     7441 tcttatgaac atcgaaaacg acgacgacag ctcggacgac gacgggaaga ccgcaacgtt
     7501 cccgttcctc aaccggcttt tcggcgcgca gaacgccgcc aacgccgaaa accgcgacaa
     7561 cgctgccgag accgagcgtc cccgcgtcgg caaggacggc ggcgagcgca agccgaagcg
     7621 caagtttctc gataactact gcatctcgct caccgaccgc gcccgcgcgg gcaagctcga
     7681 ccgtatgatc ggccgcagcg aggagctcga gcgcgtcatc cagatcctca accgccgcca
     7741 gaagaacaac ccctgtctca tcggcgagcc gggcgtcggc aagacggcga tcgccgaggg
     7801 gctcgcgcag cgcatcgcct ccggcgacgt gccggcaaag ctgcgcgaca agcaggtatt
     7861 tctgctcgat ctcacctctc tcgtcgcggg aacgcagttc cgcggccagt ttgagtcgcg
     7921 catgaagggt ctcattgaag agatccgccg cgagggaaac atcatccttg tcatcgacga
     7981 ggtgcacaat ctcgtcggcg cgggcgacgc cgagggcagc atgaacgccg caaacattct
     8041 caagcccgcg ctctcccgcg gcgagattca ggtcatcggc gcgacgacgt tcaacgaata
     8101 ccgcaagcac attgaaaagg acgccgcgct tgagcgccgc ttccagcccg tgaccgtcgc
     8161 cgagccctcg ctcagcgaca gcgtggagat tctcaagggc atccgcaagt attacgagga
     8221 cttccacggc gtgaagatct ccgacgagat gtgccgcatg gccgtgacgc tctccgagcg
     8281 ctacatcacc gaccggttcc tccccgacaa ggccatcgac ctcatcgacg aggcctgctc
     8341 ggacgtgaat ctccacaaca aggacatcga gcgcagcgcc gagatcaaaa aggaatgcgc
     8401 cgatctcgac aaggagcgcg agcttctgct cagcagcacg gcgggcgacg gcgactttga
     8461 aaagcttgcc gagctgcgca gccgcgatct tcagctcaag gatgagctct ctcagatcga
     8521 agccaagggc atgccggagc tcaccgcgga caatcttgcg cacatcatcg agctctggac
     8581 gaagatcccc gcttcgaaca ttcgcgccga cgaatttgag cgtctctccg gtcttgccgg
     8641 acggctcaag gcacacatca tcggccagga cgaagccgtg aacgccgttt gcgccgctat
     8701 caagcgcagc cgcgcgggat tacagtccaa gcgcaagccc gtgagcttca tttttgtcgg
     8761 ctccaccggc gtcggcaaaa cggagctcgt caagcggctc gccgccgatc tcttcaactc
     8821 ccccgaatcg ctcatccggc tcgatatgtc cgagtttatg gaaaagttca gcgtttcgcg
     8881 catcatcggc tcgcctccgg gctacgtcgg gtatgacgag gccggacagc tcaccgagaa
     8941 gatccgccgc aagccctaca gcgtcgtgct tttcgacgag atcgaaaagg cgcacccgga
     9001 tgtgatgaac atcctgctcc agatcctcga cgacggacgc atcaccgacg cgcagggccg
     9061 cacggtcaat tttgaaaaca ccgtgatcgt tatgaccacg aacgcgggca gcgacaagcg
     9121 caccggcagc gtcggcttca atatgagtgc cgacgagcag ggcaaggaaa aggctgtcaa
     9181 ggctctcaac gacttcctcc gccccgagtt catcaaccgc gtggacgaga tcatttactt
     9241 ccaccgtctg acggaggaaa acatccgcgc gatcgcctcg cttatgcttg aggatctccg
     9301 caccgcgatg gccgaacgcg gcaccgccct cacctgggac gagagcgtca tcacctatct
     9361 tgccgaaaag ggctacagcg ctgcctacgg cgcgcgcaat ctccagcgtc tcatccagaa
     9421 ggacgtggag gacgccatcg ctaccgagat catcgatcat ctcaagggcg cggcaaaaac
     9481 cgtcgggctc accgtacagg acggaaagat cgtcgttctc gcagtgtaag ccctatatac
     9541 gataaatgcc tcacgcagga atgatcccgc gcgaggcatt ttcttcttgt atggggtttc
     9601 agtccgcgcg gcaatgcgcg ccgaggcttt ctttgcgttc cagcgctgcg cgcgtgacgc
     9661 cctccgccgt aaggagcata ttgcgaagct cgttccactc ggcaagagac gccgggcggc
     9721 gctcgcgcag agcggccttg atcttgtcga tctcgccgag ggcctgctcc atcccctctt
     9781 tcgtgcggat aacggaagca tccttctgca tggtctcacg cagatcgtcg cgcaggtcgc
     9841 ggagcggatc cgtgccgagt tccggatacg tcgaaaggac acgtccggct gccgcagcgg
     9901 tttccggcgt atcagcgtcg gccgcggcgt attccgcggc attttttccc gcgatcgcgc
     9961 cgaagacgac tgtttccgtc agcgcgctcc cggcaagacg gttcgccccg tgcagcccgc
    10021 cggtgacctc gccgcaggca tagaggcccg caacgtctgt ggagcagtcg gtgcgcagcg
    10081 gcacgccgcc catgaagaaa tgcgccaccg ggcggatgag cagcggctta tccgggttat
    10141 cgcgcgccat gcgcgcgatg ttggggcact cgcgctcgag atatgcctta tcgcagccgg
    10201 aaagatcgag ccgaacttcc ttttcggcga actcggcgcg cgcgacaacg tcgcggtttt
    10261 ccagctcctt catcggatac ttggcgcagt ccataaaccg ctcgcccttc tcattgcgga
    10321 gcaccgcgcc gtggtcgaaa aggtccggga agatatccgc ccgcttgggc gagtaaatgc
    10381 ggtaaggata aaactgcaca aactccatgc cgcgcagcgg cagaccgcag cgcgcagcca
    10441 tcgcgtagcc gtcgccggtc acgtcgcgcg tgttgctcgt tgcggcgtac acgttgcccg
    10501 cgccgccgca gcagaggatc accgttttgg ccggatatac gcgcatctcc ccgccgcaga
    10561 gcgcaaggac gccctgtgcg cggccgtttt cggtaagaat atcgcagacg gtcacgccgt
    10621 cctccacggc gatatcggga tactcgttca gcttctcccg cacggccttc gtcagaatgc
    10681 ggccctgctt gggcgagcag caggcgagat acggatattc cccgccgttt tccgaaagcg
    10741 tccggtattc gagcggcagg ccgagaccgc gcagccgctc catggccgcg gcagcgtgat
    10801 cgacaaagaa cgccgcgatc tccgcgtcct gctgtcccgc gccggaagca aggaagcggc
    10861 ggcggtaatc ctcgcgcagg ccgggcgcgt ccggcgcgaa gcggtgcacc gacatggcca
    10921 ccaccgaatt gccggagccg ccggtcttgc tcttgcagag aatgcgcacc gcagcgccgc
    10981 tctccgcggc ggcgagcgcg gaatacatac cggccagacc gccgccgatg atgagcacat
    11041 cgctgcacgg cccggtttta tacgtaaaag atgttgtttc catttttatc actccttcga
    11101 gaatagtata ctacattctg cagaaaaaga caaaccccat accttgcggt atggggtttg
    11161 cttttcgggg gctgatgatg acaaaatgtt ttctgatgaa agaaattatt cggccttgcc
    11221 ggtggcttcc ttgttccagg acttgtcaag agccttgctg agaccccagt acaggaagaa
    11281 agccacgacc atgctgacga ccgcggggat gaggaagttg cggttgacgc agatctggcc
    11341 gatgacggtg gcgatgatcc aggagacgat accgacccag ttgatggtct ggttctcctt
    11401 ggagttcttc acgcaccact gctccgcgat catcatggcc gggatgggcg gcaggaggac
    11461 cgacagagcc ttgacgacgg gcagaaggta cttcaccgcg ccgacagccg cgaagatggc
    11521 ggcgagggcg gccacgatga tggcaagcca ggcgtgcttg accttgcctt cgagcttcgt
    11581 gtcaacgaag atattctgca gggagaggga cgcggaatag atgttgttgt cctgcgtcgt
    11641 ccagacgcag aagatggcac agacgaacac caggatgccg aggccgagag ccgcggtagc
    11701 ggcgacgaag ctggagttgc cggtggcctg cgcggtcatg gcgccgacga gctcgatcat
    11761 ggcgaggccg acgtacacgg cgatgggcgc agcaagagaa acgtgcttgc cgttcttcgc
    11821 atagcgggtg acgtccggcg tgacgatgca gccgaagatc cacgcgccga cgatggacgt
    11881 ggagccggcg gcgaaggtca gctggccctc accggggaca tagttgataa ccgtgccgta
    11941 gccggtcttc gtgccgatgg caatgaagga cgcgatggcc agaatgacgg cggcgggagc
    12001 gccgagccag ctgacgacct ccagaccctt cacgccgcgg atggcggact gggcccagat
    12061 ggcaacaaca atgatgccgg tcagccacac gggcaggggc caccacttga agttggtaag
    12121 gatcatattc gcaaaggtgt ccgcgttcac ggcgatccag ttgacggcag acagagcgat
    12181 cagcacggac atgatggccg agctgcgctt gccgaggaag ttgcgggcga gaaccagcgt
    12241 ggtcttgccg ctcttcgcgc cgatgatacc gaccatgacg gcgacaaaag caaggatcgc
    12301 gttgccgacg acgatcgacc acaccgtgcc gctgaagctc atcttggagc cgaccgtggc
    12361 gccggtgacg aagttggaaa gagaaatgca gtagccgaca agaatgacca gaatggtgct
    12421 gaaggacttg cgtttgtccg cgggaacgcg accgaacgca tattcgatct cagaaccgcc
    12481 ctgctctgca gctttctttt cgggctcttt ggacatgaaa aaaactcctc tcgatctttt
    12541 tacgaaatgt gcgcctccga aagacacatt tcgttcacat tttcggatta tagcactttc
    12601 cataccaaat acaaccccta ttcgctgtca aagaccataa atcgtcatat tatccatata
    12661 ttattattaa attaacaagc ttttattgac actccgttta taagatgata tagtttattt
    12721 gaataagctt ttattttgct atatattttt taaatacaat cacaggatat aagagggaaa
    12781 tgatatgaac aaccccggcc gcatccaccg cgcgtggtgg atgctgatcg gctgttgtct
    12841 gctgcagggc ggatcgctcg gcatcgttca caactgccgc ggtattttct atgcccccgt
    12901 gatcgaagat ctcggcttcg gtatgggcgc tttcactttt tatgtgctct ttttcggcgt
    12961 ctgttcgtgt attgtcctgc cgtttgtcgg aacgctcttc cgccgcatcg attcgcgcat
    13021 tctgctcggc ggcgcgtcgc tcgtattttc cggcacggtg ttcgccatgg gctttttccg
    13081 gacgcttccc gccttttaca ttgccggagc ccttcagggc tttgccggcg cgtttctcat
    13141 gttttacccc gcgccgtaca ttctcggcaa ctggttccat aaaaaacgcg gccttgccgt
    13201 cggccttgcg gcggcgttct cggggatcgt cggggggctc ggcaatccgc tcggcaatgc
    13261 catcatccgc accttcggct ggcgggccgg ctttttctcg ttcggcgcta tttctctttt
    13321 gatgatgctg ccagtctcgg tctttctgct gcggtgctcg ccggaggacg tcggctgccg
    13381 gccttacggc gccgaggacg atgaaacgct caccggtgag agctgtccgc tcgacggcgt
    13441 tccggcctcc cgcgccaagc gggagagcgc tttctggctt ttgatccttg cgggacttct
    13501 tgcgtcgttt acctgttcat actgctcgca cctcagcccg atcggcatat acttcggcta
    13561 tggttcggcc gtcggcgcgc tgatggtctc ctgctccatg gcgggaaacg ttgtgggaaa
    13621 gctcctgctc ggccatctct acgaccggtt cggccttcgc gtctcgctcg ccgccggcac
    13681 ggcggtcaca gcggcgggct atctcctgct gctcataaat tcgcttacgg ctcgcatcgc
    13741 cggcgcagcg ctctacggct tctccatggc gacgagcacc gttatgatcg ccattgccat
    13801 ccgcgatata tacggcagcc gggactgcgg cgagcttctc tcctacagct ccatggtgtc
    13861 cgcgctcgga acctcggtca acgccgtcat cataggctat atggtggatg gcttcggccg
    13921 gcagcgcgga tacatcatca gcttctggta cggccttgcg ctgacgcttg ccatgggcgc
    13981 gctgttcatc gtatccgtcc gccgcggccg gaagatcgcc gcggaataca agaccggcgg
    14041 gaacgccgga gtctcatcga aaccataaag aagggctgga attacctttg aagcacgatt
    14101 cattgcatca gcaggcgatc cgggagatca cggagcgcct catttccacc ggaaacatgg
    14161 acgggacgct ggaaaactgc cccggcatga aggagcgcgt tgcgcgcctg atcgcgctgc
    14221 tgcgcagcgc catgtacccg catattttcg gcacatcctc ccggcggggc agcgccgcct
    14281 ccgccatcgc ggtgagctat aacctgcaga gcgccgcgga gcttctcgag cagatcctca
    14341 caacgctcca gccggagcgg accgtggaag agaccgacac gctcattctc cgctttcttg
    14401 agcagatccc ggagatcaaa gcgtgcctcg aaaccgacat tcaggccgct tacgacggcg
    14461 accccgcggc gatgagctgc gacgagatca tgctcgcc
//
LOCUS       DAWAAE010000036        14446 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1883_length_14446_cov_3.331110, whole genome shotgun sequence.
ACCESSION   DAWAAE010000036 DAWAAE010000000
VERSION     DAWAAE010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1883_length_14446_cov_3.331110"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3275..3284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13627..13636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2913 a         4555 c         4067 g         2891 t
ORIGIN      
        1 catttacgac gactacattt tccgcttcca ccccgatttt gaaggtctgg tcaagcgcta
       61 ctacgaccgc gacggccacg ccaacatcga gggcggcgac gtgctcaacc tcaccgagga
      121 cacgctcgcg atcggcatct cccagcgtac ctctccggac gccatcgagg aagcgtcccg
      181 caatctcttc gccgatcccg aagcgaagat ccgcaccgtg ctcgcgttcg atatcccgag
      241 ctgccgcgcg ttcatgcatc tcgacaccgt gttcacccag atcgactacg acaagtacac
      301 gatccatccg gcgatcctcg ggcctctcac cgtttacgag atcaccccgg gcgtgagcga
      361 tccgaccgat ctccacatcc gccgcatcga ctccaagctg gaggacattc tcgcgaagta
      421 cacccgcacc gaccacgtca agctcatcaa ctgcggcggc gacgatctca tcgcctcggc
      481 ccgtgagcag tggaacgacg gctccaacac gctgtgcatc gcgccgggca agatcgttgt
      541 ttacgagcgc aacgacgtga ccaacgccat tctccgcgac gagggcctcc aggtcctcga
      601 aatgccgtcc gccgagcttt cccgcggccg cggcggcccg cgctgcatgt ccatgccgat
      661 catccgcgcc gaataatttt cattcgctga ataaacccat tcatataaca cacattacaa
      721 tcatttccta taggagggta aattattatg ggtctcaaca ttcgcggcag aagcttcctc
      781 actcttcttg acttcacccc ggaagagatc aactacctca tcgacctcgc cgttgagttc
      841 aagcgcctga agaacaacgg tgtggagcac aagtggctct ccgacaagca gatcgtcctg
      901 ctctttgaga agacctccac ccgcacccgc tgcgcctttg aagtcggcgc gcgtgatctc
      961 ggcatgggcg tcacgttcct cgattccggc tcttcccaga tgggcaagaa ggaatctctc
     1021 gccgacaccg cgaaggtcct cggccgcatg tttgacggca tcgagtaccg tggcttcaag
     1081 cagtccgtcg tggaagatct tgcgaagtac tccggcgttc ccgtgtggaa cggcctgacc
     1141 gaccagttcc atcccacgca gatgctcgcc gacatcatga ccgcccgcga agagttcggc
     1201 gatatgcgcg gccgcaagct taccttcttc ggcgacgccc gcaacaacgt tgccaactcc
     1261 ctcatggtcg tcagcgcgaa gctcggcatg cacttctgcg cctgcggccc gaaggagctc
     1321 atgcccgagg agtggctgat cgagaagtgc caggccgttg ccgccgagac cggcgcggtg
     1381 cttgagttca ccgacgacgt cgcacagggc gcgaaggact gcgatgttct ctacaccgac
     1441 atctggctct ccatgggcga gcccgccgag ctctgggaga agcgcatcaa gctgctccgt
     1501 ccctaccagg tcaacaagga gctcatggcg atggcgaagc ccaccgcgat cttcgagcac
     1561 tgtctgccct ccttccacga ccgtgagacg accgtgggcg aggatatcta ccagaagttc
     1621 ggccttgagg cgatggaagt caccgacgac gtgttcctcg gccaccaggc ccgccagttc
     1681 caggaagccg aaaaccgtat gcacaccatc aaggctgtga tgtacgccac gctgaagtaa
     1741 gggatcgaaa caatggctga aagaattgtt gtcgctctcg ggggaaatgc tcttggcaag
     1801 acccccgagc agcagctcga acttgtccgc aacaccgccc gcccgatcgt tgatctcgtc
     1861 gagaagggct acgaagtcat catcggccac ggcaacggcc cgcaggtcgg catgatcaac
     1921 ctggcaatgg agttctccgc caacaagggc ggcggaacgc cgttcatgcc gttccccgag
     1981 tgcggcgcca tgacgcaggg ctatatcggc taccacctgc agcaggccat tcagcaggag
     2041 ctgaagagcc gcggcatgga gaaggaatgc gccaccgtcg tgacgcaggt cgtcgttgac
     2101 gagaaggacc ccggcttcca gcacccgaca aagcctgtcg gcagcttcta tacgaaggaa
     2161 gaggccgaga agatcgccga ggaaaaaggc ttcatcttcg ttgaggacgc cggacgcggc
     2221 taccgccgtg ttgtcccctc tccgatcccc aagcgcatcg tggagctgaa cgttgtcgat
     2281 cagctcgtgc gcgccggtga tatcgtcatc accgtcggcg gcggcggcat ccccgtcgtt
     2341 gagaccgagg acggtctgcg cggcgtcgcg gccgtcatcg acaaggaccg cgccagctcc
     2401 ctgctcgcgc aggacgtgcg tgccgaccgt ctcatcatcc tcaccgctgt ggacgccgtg
     2461 tgcgtcaatt acaataagcc cgaccagaag gagctctcct ccatgacgat tgccgagtgc
     2521 gagaagtata tcgccgaagg ccagttcgcc cccggaagca tgctgccgaa ggtcgagtcc
     2581 tgcatggagt tcgtgaagaa caacgaccat ggcggaaccg cgctcatcac ttccctggcc
     2641 cgtgccgccg acgctcttga aggcaagacc ggcacggtca ttacccggta agcaaatttt
     2701 tgtcccgttt cgcatctccc agcgaagtcc gaacaaaatt ttaagagagg ggttcaaatc
     2761 atgtctaaga agaccaaaag atccatcagc acatttgcga tcctgctcgc cgttatgctc
     2821 cttgtgtcga tcgccacgtg gctcgcagcg ggcaagacct acaccggtga gaacggcgag
     2881 atggccgaag tcgtcggcgc ctccgccagc gacatcttta tggggcctgt caacggcttt
     2941 gccgacgcct ctgacgttat cacgttcgta ttctgcctcg gcgcgttcct tgccatcgtg
     3001 aacgccaccg gcgctcttga aaccggcatc cacacgctcg tccgcaagct gcacggcaaa
     3061 gagctcgtgc tcatttggat tctcatgttc ctgttctccg tcggcggcac gacctacggc
     3121 atgggcgaag agaccgttgg tttctacatc ctgctcgccg ccacgatgat ggccgccggc
     3181 tttgacccga tcgtcggcgc ggccaccgtc ctgctcggcg ccggcaccgg cgttctcggc
     3241 tccaccatca acccgttcgc gaccggcgcc gccgnnnnnn nnnnctgccg cgacctccgt
     3301 cggtgtcgaa gtgaacatgg gcaccaccta tgtcatcggc atcattctct ggctctccgc
     3361 ctaccttctc tccgtgctgt tcgtcaccgc gtatgcgaag aaggtccgcg ccaacaaggg
     3421 ctccatcctc accgccgcgc agctcgacga gtgccagaag gcttacggcg gcaaggacgt
     3481 caccaccgcg aagctcaccg gccgccagaa ggccgtgctg tggctgttcg ctctctcctt
     3541 cgtcgtgatg atcctcggct tcattccctg gggcagcctg agcgaaacca tgtacaacgc
     3601 tttctcgagc tccaccactt ggctcaccgg cgcctgcctc ggcgactggt ggttctatga
     3661 cgccgccacc tggttcctgc tcatgggcat catcatcggc atcgtcggtc tcgaggacaa
     3721 gggccagctc atgaagctca tcgccaacgg cttcagcgac atggtctccg tcaacctcgt
     3781 tatcgcgatg gctcgcgcca ccagcgttat catggccgcc accggtctcg gcacctggct
     3841 ggtcgaagcc tccgtcaccg cgctcagcca gtccggcgtt tccgcgggtg tgtttgctct
     3901 gcttgactac atactccacg tcggtctcag cttccttgtt ccgagttcct ccggtctcgc
     3961 cggtctgtcc gccccgatcg tctccccgat cgtcgccggc atgggctgga gcgtagagac
     4021 ctccatcatg atcaacgttg ccgccaacgg cttcgtcaac ctcttcaccc cgacctgcgg
     4081 cttcatcatg ggcggcctcg cgctggcgcg cgtcccctac gagagctggc tgaagtgggc
     4141 gtggaaactg ctcctcacgc tgttcgtcgt cgtcggcgtc gtccttacga tcgcgatggc
     4201 ggtgctgagc tgaatttccg cttgagttat cgcatgatct aatggtaaaa taccggttgt
     4261 acgccccatg gcgtacaacc ggtattcttc aaacaagagc cgcttcgcct gctcttgttt
     4321 gagaagggct gcggctcgcc gcagcgcacc cgctttgccc gcacgtttgc gagacttttc
     4381 agtcatttgt aaaaaaggac ggtgcagatt atgcccagac gagccatgct ccaggatgcc
     4441 cgcgcctccg cgtccgaccc cgccgtgatc tgctcgttcg attacgaggc gatcgacaag
     4501 cccacccgcg cgatcatcca tcaggcggca cgctttcttt acgtaaagcg cggccgcggc
     4561 aagctcgtca ttgacggcga ggagtacgtc ctccagccca acacgctcgt cgccatcacg
     4621 ccgtggaaga tcaccgacac ggtagaagtg acggaaacgc tccagttcat caaggtcata
     4681 tacgactatt cctatctcaa ctctgtattc aagagcgttc tcggctcggg cgatgcttcc
     4741 gccgagcccc tgcgctttct caccgtggag ccggtcgcct atcttgacag cgtacaggcg
     4801 gagtatgtgg acggcatcat ggagcatctc aaggcggagc tcggcgtgga atccacgcgt
     4861 gtcaagcctc cggacaagcc gttttcgcag ctctatacgg tcaacaagct catcgatctg
     4921 atggtcgttt accgccggta tatcatgtcg caccgcggcg agctggactc cggacgggag
     4981 gagaccgtca aggaccagtc cgttttgagc tacatttatg cccattccgc ggaaaagctc
     5041 acgctctcgc aggtcgcgga ggcgttcttt ctttcggaat cggcgctctc caagcagatt
     5101 gccggcatga ccggcacgac gttctcgaag ctcctgagca gcatccgcgt ggagaaggcg
     5161 tcggactatc tcatctatac ggatctcacg ctcgacgaga ttgcggggct ctgcggcttt
     5221 gtggacgcct cgcacgtgtc caagcacttt gcgcagcgcg tcggcatcac gccgatgcaa
     5281 taccgcaaga tctattccaa ggccgtcacc aagttcaaca tctccgacaa ggcgatcgcc
     5341 tttgcgctga cggactacat ctacaaaaat tatgagaccg aacggctcac cgccgcaagc
     5401 gtcgccgccg aattcggcgt gtcggtgccg gaaatgaacc gtctgcttct ctattataac
     5461 gagatgaact ttgatacgct gctcaactcc acgcgcatca ataaagcgtg cgaaatgctc
     5521 gtatcgacgg actatctcgt gatcgacgtt gccgtcgccg tcggctacag caacatcaaa
     5581 acgttcaaca tgaattttta ccgcttcaag gaaatgacgc cgacggagtt ccgcgaccgc
     5641 ataacccttc agcgcacaga cggcagcgaa acgcacggaa agggccgcgg cgcgcgccgc
     5701 cgcacggtgc ccgtccgggc accggaaacc gcaaaggaga cgacagaatg aaatccggaa
     5761 gacagggcac cattctccga atcatttcgg agcagagcat tgaaacgcag aatcagctca
     5821 tggacgagct gaaaaagcgc ggcattgaca ccacacaggc tacgctctcg cgcgacatcc
     5881 gcgagctccg tctcgtcaag cagcgcagcg cggacggcac cagccggtac gccgcggcgg
     5941 aggtcgcgcc cgcgccggag atgagccagg ggctcaagga aaatctccgc cagaccgtga
     6001 cgggcgttta tgcctgccag aatctcgtca tcatcaagac gctgccgggg ctcgcccctg
     6061 cggtgtgcac cgccgtggac ggcatcgctc ccctgccggg gcttgtcggc tcgctttccg
     6121 gcaacgacac ggtgtttctc gccatgcgcg acaacgccgc cgccgaaacc ctcgcccgcg
     6181 aaggccgcag cctgttcatg ggctgaatcc cggataaccg accatccccg ccggaaactc
     6241 tatccggcgg ggatggtttt ttgtgtgttt ctttgcgcct gcacgttccg gccgccgtcc
     6301 accgctgcca tttgcgccgc ggtgatacaa ccgtgatttt gcggttttcc gcgtttttgc
     6361 gcgcaaacga tcccgtcagg aaattttccg gacgggatcg ttttttcttt attcgagatt
     6421 gaggcgcttg ctgaggatgg cgcgggtggc gaagaagaag ccagtgccta cgagaagatc
     6481 aaggccgatg tagatgccga tcgcggaggc catcatgccg acgccctgaa catgccagcc
     6541 ggtatcgtcc aagcagaact cccagttcaa cagcacatcg ggaaatgcat cggactggag
     6601 gagcgacatg cacccattca taataatgct gaaggcgatg ctgatgccga cataggcaag
     6661 cagcgcaaac catccgcggt ttttctttgc gagatgcccg atcgaaatgg cggtatagat
     6721 ctgcaaaatg gtgacggcag cggcgatgag catatacacc gcaaactcgg cgatcagcca
     6781 gcccgttccg gcaggcagcg ggtttacgcg cagatagcgg cccgcttcct ggaacgcctg
     6841 cgcaacgtcg ctgaagttga gcggcgtgag cacagccatc aaaagcaccc cgctcagcag
     6901 cgcgacaatg gcgctcgcca cggaaaagat catgctcgtg agcgctttgg aggcgatgtg
     6961 ctccgagacc gacacgggca gcgtgaacat gaggtaccct tcgtcgccga gaaggccctt
     7021 ataaaaccgg ttgatgatga aaacgagcat gaaaatgccc atcgccacat aaagcgccac
     7081 gagcaggccc attggcagca cggtgataat aaagcgtgcc agcttgcttt ggatatcgcc
     7141 gtcgaacgtg atccgaagcg tgagaccgtt gatcgcggca agcgccgcca gaccgatcca
     7201 gatcagcgca aacgtgcgga taccggagcg cagatcgtat ttcatcagtt taccgagcat
     7261 tgtattctcc tccattcccc atgaccggcg cgcggaacat cttgcggaac agctcgtcca
     7321 cgctgccgcc ggtgcgctcg cggatgctgt cggtgctctc gtgcaggacg acctgtccct
     7381 gctggaggaa caccgcctcg tcgagcacct gttccacatc gaggatcaga tgcgtcgaga
     7441 tgagcaccgt accgttttcg ttataattcg tcaggatcgt gcgcagaata aactcgcgcg
     7501 ccgccggatc gacgcccgcg atcggctcat cgagcagata gagccgcgcg cggcgcgaca
     7561 tgacgagcat gagctgcatc ttttcgcgcg tgcccttcga gagcgttttg atcctgctgc
     7621 ccgcttccag accgagcacg cggcacattt cctccgcctt ggcgcggtca aagtcggcat
     7681 agaagtccga gaacatatcg atcatatcgc cgacgcgcat ccagtcgggg aaatatcccc
     7741 ggtcgggcag atagcttgtg atcgcctttg tctctacgcc gggcaggaga cccgcaacgc
     7801 gcgcctcacc cgccgtcggc tgaagaaggc cgttgaggat cttaataagc gtcgtcttgc
     7861 ccgagccgtt cgggccgagc agaccgacga tcttgccgga tgtaaggctc agatcaacgc
     7921 cgcgcagcgc ctgcaccgct tcaaagctcc ggcacagccc ggtgcatttt accagttctt
     7981 cattcatcgt tctttttcct ttccgcctgc gccatatccg cggcctcacc gcaggaatag
     8041 cccagcgctc ccatttcctt gaaaaagttt tccgtgcact ccacggccag ccgccgccgc
     8101 atctcctcga gcgccccggc gttttccgtc acggtgcggc cgagcgtccg gttcgttgtg
     8161 acgaggccga gcgtttcgag ctgcgtgatc gcgcgctgca tcgtgttcgg attaacgccc
     8221 gcctccgccg ccagctcccg caccgcggga aagcgcgacc ccagcggata cgcgcccgtg
     8281 atgatccgcc gcgccagctg ctcggtgagc tgctgccaga tcggccgcga gccctgaatc
     8341 gcaaagtcca tgccagccct cctcgttgta ttgctgtatt aagcgattag tacactacta
     8401 cactcataca gttttgtcaa gccccttttt taatttttcc tgtggattcc gcgcagggaa
     8461 tgtggtaaca tgaaaatgtt caaataatct taaaattttt ttgcttttta ctgaaccgcg
     8521 tgttgggaaa aaccgtcttt ctttacagaa aggcgtagag cctctcagag agagtttgcg
     8581 tatgacagcc gaagagttta ccgtccagat catggacatg aaaaaaacgc tctaccacgt
     8641 ttcctgcggc attctccgca gcgaggctga ccgggaggac gcggtgcagg agtgcatatg
     8701 caaggcgtgg gaaaagcgcg attcgctcaa aagcgcgggc gcattccgcg catgggtgac
     8761 gcgcattctt atcaacgaat gctacgacat ctgccgccgg gggagcaaga tcgttcttct
     8821 cgacgagatg ccggaggcgg tcgcccccga gagcggcgac cgcgagctgc gcgacgccgt
     8881 gatgggtttg gatgagatat accgtatgcc gatcctctta ttttatgtag aggggttcag
     8941 tatacgggag atatcccgta ttttacagat ccccgagggt acggtcaaat cccggctgtt
     9001 tgccgggcgc ggaaagctcc gcgaagtttt gggtgaggag gtgccggtat gagggattcc
     9061 tgggacaata tgtttgaaga ggttcccgca tcgtttgagg cacggatgaa ggaaacactt
     9121 tccgcgctgg aggaaaagcc gaagatacgg cgcttccggt tcggtacggg cgctctcgtc
     9181 gcggcggcgc tgctcgtcgc ggtgctcggt ggcacggcgc tggctacgga ctttttcggt
     9241 ctgaagagtc tcaccgtcac cgacccgaac gcctcccccg ccgcggtctc cgccacggcg
     9301 gagccggtga gcgacagcac ggtcatcgcg ctgcagggcg tgccggggag cccggagttc
     9361 aaggcaaacg ccgagtggat ggacttcctc gcgtcgtacg acaccgccgc catggcgaag
     9421 gaaaccgccg cctgcctgcc ggagaagtac gatctctact ccgtctccac gcaggacatg
     9481 gccaacgagc tcgaccagat cacccgcaag tataatctgc ggctgcacac gtccgtgacg
     9541 gactttgaca gcgaggatac gctctactcc ctgctgaacg ccgaaccgtt ccttaccgac
     9601 tgcaccgccg tgagcggata tatttacgag gacggctcct tccaggccta cggcacaaac
     9661 gtttccggca agagctacga atatcagctt ggccggtacg tcaagggcgc gttctccgag
     9721 gtcacgctga acatcggcag cgccgaggat tatgaggagt ggatctacac gacctcccgc
     9781 ggcgacgagg tacagctctc gctcggcccg agccgctgcg tcgtgatgtc cgaccagccg
     9841 aaggcgttcg tggccatcaa cctgctcggc ggcacggagg gcaacagttt cttcatgccg
     9901 gagccggtca cgaaggaaga tcttgaggcg ttcgcggagc tctttgattt tgcgaagctc
     9961 ggataatacg aaatgataca gcgcaccccc gcgggaaatt tcccgcgggg gggcattttc
    10021 attattcgta cagcagcttt tgtctctggt agggctgcgg ctcgtagcgg acggagccga
    10081 tctgcggctt cggcgggccg tatttggggt tgtagccgaa aaggtttatg taccgctcga
    10141 tgtcgccgcg ctcccgctcc atggcgagca gcacctcgag cgcagcgttg gcgtcgtcca
    10201 cggcgcggtg ggagtttacg ccgtctacac catagctctc cacggcgttg cagagcttgt
    10261 gcggatagtc ccgccggtct ttgtagatcg ccatgatgtc gagaaactgc gccttttgca
    10321 gcaccccgtc gcagccgagc cggtgcagga agtaaaagag gaagcacaga tcaaactgcg
    10381 cgttgtacgc gcatacgagc ggcttttctc cggcgagcga acggacgaac gcctccgcag
    10441 ccacgtcctt ttccacgccg ttttgaaaaa gctcatcctc acggatgccg gtgagctgcg
    10501 tgatgaccgg cggcagacgg cggcccggcg agaggcggat gagataatct tcctccgtgc
    10561 gctcccccgc ccggtcgagc cgcaggaagc caagctcaat gatctcgtcg gatctgcagt
    10621 tcacgccggt cgtctccgta tcgagcacga tgacggaatc gtaggtttga aaaaggcttt
    10681 caagcattcc ttattctccc ttcgtcagca ccgcgccgac cagtacaaag tattccggcg
    10741 gtgcgtcctt tgtcccccat tggagcggcg cgcccagctt gtccgcggcg agcgcgccga
    10801 cgttcgcgcc gagcgattcg agcgaatagc ggagcttttc cggaaaccgg cagggctcct
    10861 tgtcccggcg cgcgcatcgc tcgcacacga ggcagcgccc ggcgtccagg cgaagggaac
    10921 cgcacgtttc ccgctcccgc tcttcgagca gcgcatcaaa cgctctccgg tacgggcaga
    10981 ggaacatctc cgggtcggcg agtgccgcgg ctttcgcctc ctcgctctcc gggaacacct
    11041 gcacggcgta cagcgtgacc gcggcatact gcggccagac cgccgcgggg tcaaaatcgt
    11101 agggcgggca ggaccacgtc tgcccgcagc gcgggcacgc ggcgcaatag ccgaggaatt
    11161 tttccacgtc cacatacttt tccagcatct cgctcatcgg cagcgcgccg gtgaggtgct
    11221 ccacgcgcac gccgttttct ctcttttcca tgggtatctc cctcatttca gcgattcctg
    11281 atggtttacc acacccttga acggcgtcat aagcaggatc ttgatatcaa acagcatcga
    11341 ccagttttcg atataataga gatcgtaatc cacccgcgcg cggatggagg tatcgccgcg
    11401 cagaccgttg acctgtgccc agccggtgat gccggggcgc acctgatggc gcaccatata
    11461 aagcggaatg ctgtatttaa aatgatcgac gtaccgcggg atctccggcc gcgggccgac
    11521 gagcgacata tcgcccgcaa ggacgttaaa tagctgcggc agctcatcga tggagtagcg
    11581 ccgcatgaac gccccccacg ccgtgcggcg gggatcatgg tcgcgcgtcc acgccgtgtc
    11641 cgactgcgcg ttcacgcgca tggagcggaa tttgagcata taaaagggct tcttgtcctt
    11701 gccgatacgc tcctgccgga acaggaccgg ccccggagag gaaaggcgcg tgccgatcgc
    11761 ggcgacgagc atgacgggcg atgtgagaac aatgagaaca agactcgcga gaatatcaaa
    11821 aagacgcttg acgatgtccg cgccggcatt gtcgagcggc acacgccgga cgttcaccag
    11881 cggcagaccg cacagctcct ccacatacgg gtggcgcgac atatattcat acccgatcgg
    11941 cagcagcgcg agcttcacgc cggtatcctc gcattcgcgc agtatcccgc cgagccgttc
    12001 cgcctggttt tcctccatgg cgatcaccgc ctcgtccggt gcgttcgtct caagcgccgt
    12061 gcgcagagaa tcataagagc ccagatgcgg cacgccgctg agcgccgatg cgcccacgct
    12121 gccgatgacc gtccagccgg cgtcgcgtcc cttcttcacg cggcgcagac attcgcgcgc
    12181 cgatgcgccg tcgccgatga tgacaaggcg gcgcagccca tttccggcgc ggtactgccg
    12241 gcggagcaga cggtgcgcaa taaagccgcg cgcgccggtg aacgcgatga gcagcagata
    12301 gcagagaagg atcatccagc gcgaaaagtc caccacgcgg aacacgaaga tgagatcgat
    12361 atacagcatc acgccgaacg tgcagccaag aacgatgttc ccaaattcgt ggatgaagcc
    12421 gcgctgcggc tgcggctcgt agatgccgag cagcccgaag agcacggcgt acaccggtga
    12481 gaacagcccc gcccagagca tatgatactc gaagccgccg accggatttt cgccccggaa
    12541 cagcacgaac cggataaaaa acgccgccgc catcgccgcc acagacagcg ccatatccgt
    12601 tgccgcccag aggacggtct tttttgtttc tcgtttcttc atcgcatttt ccgccgtata
    12661 ggaaagtttt gtcagttttt agacggtttg ccgcgcagcg cggttcgcct ccgcgcgctt
    12721 acgcagctat attttaccac aaatggtttg tttcttccac ggtttctgtt ataatccggt
    12781 gtagaaaata ccgtaccgga ggacgccatg aaccattccg cgccgaaaat tttatatacc
    12841 gcctccagca gcgcgcattt ggtgagcttt catctgccgt atctcgccgc gctgcgctcg
    12901 ctcggctggg atgtgaccgc cgcggcgaac ggcagcggcg aagatctcgg cgagggcgtg
    12961 cggtttctcg ccgtgccgtt tacgaagagc ttcgtatcgc cgaaaaatct ccgcgccgcc
    13021 gcgcttttgg cgcgggatat ccgtaagaac cggtacgacg tgatcgtgac gcacacctcg
    13081 ctcgccgcgt ttttcacccg tctgggcgtg atgctcgcgg ggaaaaagca tacccgcgtc
    13141 gtcaatacgg tgcacgggta tctctttgac ggcgattccg gcgcgctgcg gcgcacggtg
    13201 ctgctcgcgg cggaaaagct gacggcgggc gttacggacg acattctcgt tatgaaccgc
    13261 tgcgacgagc agatcgccaa aaagcaccgg ctctgccgcg gaaacgtata ttttattccc
    13321 ggcatgggcg ttgatttttc ccgcttcccc cccgccgcgc cggaggagcg cgccgcggca
    13381 agagcggcgt attctctgcc ggaggacgcc gtgtgtctcg tctgcgccgc ggaattctcc
    13441 aagcgcaaaa accagacgct gctccttcag acgctcgccc ggcttcccga gaactattat
    13501 ctcctgctcc ccggcgacgg ggcgctgctg caagagtgca aagccgaagc cgaaacgctc
    13561 ggcatctctt cccgctgccg gtttccggga cggatctccg aagtgcgcgg tgcgctcgcc
    13621 gcggcgnnnn nnnnnntttg agagtgactc cctctgccgc aaattccggg atctgctgga
    13681 gggctccggc gcaggctatc tcgttccgtt cggcgacgcg gcagcgtttg ccgaagccgt
    13741 gcagacgctc ggccgcgacg ccgcgctgcg cgcgcggatg ggcgccgccg caaacgagag
    13801 caccgaaaaa tacgccctcg ggcgcgtatt gcccgaggtg ctagcgcact tcacagaaaa
    13861 aatgcaaata taataaaaca acctgccgcc aaacaaacgc ttggcggcag gttgttttct
    13921 attctttccc cctataatga aatagagcgt tcactctctt ctcgtgagat agtacgcact
    13981 tgccccccat tcaatataca ttttctcctt gccaaagcgt atgtagaatt ccacttcgct
    14041 aagtctgttg ctaaagggat tgatatgaac tccacctgtt tcttccataa aaagaaatcc
    14101 attcgagtca tcgcctaaaa ctttcccgtc gtagatggct cctccataaa gattgctgtc
    14161 aacctctgcc catacatcgc cgaactgcag atagaattcg gcagtagcat tgtaacttcc
    14221 ctcttcgtaa aacactccgc tgcattctat caggttttca agttgttctc taaaagatat
    14281 tacattcatg ttttcacatc ttatatctgc aagcattgta tacgcatctt ccacagatat
    14341 tctggatgaa ttaattactt cattgtattt ttcctctgcg agggcttcct gttctgcata
    14401 tatttcatca tatttttctc ttgccgaaag aagagtagaa taatac
//
LOCUS       DAWAAE010000037        14416 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1893_length_14416_cov_3.308126, whole genome shotgun sequence.
ACCESSION   DAWAAE010000037 DAWAAE010000000
VERSION     DAWAAE010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14416)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_14416_cov_3.308126"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4207..4216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4599..4608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10354..10363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3083 a         4348 c         4189 g         2766 t
ORIGIN      
        1 cttaccgcag ctatgctgct gacgctgctg accggctgcg gcaaaagtag caccgaagtc
       61 cccgaagtat atctcgatca ggcggtgcag gaatttatgg aggatgagta tccacactgg
      121 gccgttgcat ccgatgaata cactgatttt aactatgtgg catttcatgc cccggataaa
      181 acgatgcata cggattccat gtatttcatc gtatcggtct gttttcccta cggcaaacag
      241 aattggtcag cagagcaggt ctatcagtat tccagaagca acgattactg gacagaaatg
      301 gacgaatggg aatggtcttt tgacgacgaa tacgtttcta acgattccat tatcggcaac
      361 acgttttccg ggccgctgga tgctaaagga agcaattata cggtaacagt cgatgctctc
      421 gactacaacg gcagcagcat cacctgctcc tataccatcc atgattacag ttactcctac
      481 tctcccgact gcagtggctc cggaacgttt cagctctata ccaactaccc gtacaaagac
      541 ggtctaaagc tcttccgtat tgtcaacgag aaggtgcaat acacgctctc cgtttctccc
      601 gtttacggcg taagaatccg ctccgggtac aacccttaaa aggagaacta tcatgaaaaa
      661 agcttttagc gtgtttctag cactgatgct ggtgctactg gtcgtcccct gcgaaagtgc
      721 ccatgccgtt gagtatctca ccatcgtatc ccgcgatgac ggcacatggc tctggccgct
      781 gtcggagaca gcatattatg aaagctgcag cgactgggcg ggctgcaatt ccaacggcag
      841 ctccggcata tgcccgttcc acggctatca ctgctataac tgttccgcga atcacaccac
      901 cgataacggc tacggtcaca acggtatgga cgtcggcgtc tcctacggaa cggcggtgta
      961 cgccatagcc gacggcacag cctactgtgt ggactataac catgattcca gaggctatac
     1021 gctcgtcatt gaacacgcga tcgacggcga atggtcgtat tacagctatt atcagcatct
     1081 gcagggcgtt gacaccgctc tgaacggcaa gaccgtaaaa gcgggcgaca cagtcgccta
     1141 ctccggcaat tccgccggtt acggaagcag cggcgattcc catctccatt ttaggatcgt
     1201 aatggggcct tccggcgctg gaagccgcct tgcctctgct ccgaacaata aagacaccgg
     1261 cctcagctgg ctggaaaact gcggatggat cacggagcag ggtctccaaa acggacgcat
     1321 tctgaataat cccgcggaaa atatcccgta tgtcagcaag acgtccgatt cgtttatggc
     1381 gcaccccggc tcagtgcatt atacctttga tgcgaacaag gtctccgtaa gctcttacct
     1441 cagtcagtgc aagttttatc cttcgtacat gaccgtagaa gcagtgaaca tcgaggacga
     1501 cacctacatc tggacgcttc cctgcatttc tcaaacggac gagcattccg agatcatccg
     1561 ccgtccgaaa aagggcgaga ccctcacggt gaccggcata ttccgcaact cggtcggaaa
     1621 ccactactgg tatcgcataa acgttgacgg gcaggtcggt tatatctggc cgaccgtcgt
     1681 gaagaacgga gatgcgatct acgacgatgt gacgatctcc aatgttcaaa cgccttcttc
     1741 cctgtccgtc ggcagccgct tttctctgcg cggagagata agcacgaagt acaatgtcat
     1801 ccagtatctg cacgccgaca ttatccgtac ctccgacaac acaacggtat gcagttggga
     1861 acaggaaata aacgccggtc attactccat atacaacagc gatctcgata tggcgatgct
     1921 cttcaacgag ctgcccgccg gtgaatacca gcttgagatc tgggcggata taccgagtta
     1981 ctccaccgtc aaaggaaacg acctcttcca cggcggcata tgcaaaacct ttgtgcggga
     2041 ttatttcacc gtatcataaa cgaaaaaccg gttcggagct tccgaaccgg tttttcttat
     2101 ggactttttg ccaacaatct gctattttca atgcgtaatc ccaaagcaaa ggagacgatc
     2161 atcatgcgtc agaataaagg ttggagaaca gaatacttct ctatgatcta aaaaggagaa
     2221 ccaatcgatt tatgctgaac aaagagggat tcaatgcatg ggcggacggg tacgaccgca
     2281 gcaccgcccg ctccgccgcg ggaaaggtca cgtttatcca aagccttctt ccgctgctgc
     2341 gcgagggcgg agtcatctat attggcaacg ttgcctttga tacacgcgcg gagctggagg
     2401 cgtgccgggc gcagtccgga acccgctggg acaagtacga gatctatttt gtctatgacg
     2461 agctgaaaaa agtgttcccc gccatgacgt ttgaccgtct ctccccctgt tccgggatac
     2521 tgtcgctgcg gaagtgaggc tgccatgacg tccaccgact ggatacacta tatacaggat
     2581 gcttacagca cagagccgga gcatccgtgg gcggacaagc cggagttttc cgtattccgt
     2641 catgcgagca acaaaaaatg gtttgctctt tttatgaccg taaaggcttc gctgctcggt
     2701 ctgccgggag ccgaagaagt ccccgtgctg aatctcaagg ccgatcctcg tctgattggg
     2761 ccgcagcggg aaaagcccgg gttcttccct gcctaccata tgaacaagga gcattggatc
     2821 accgccgcgc tcgacagcag cgcgccggag gacgaaataa agattctgct tgccatgagt
     2881 tatgatctga cagcaatgac gcgcgcttct cgcaaaaata ctatataacc ggaggcaagc
     2941 tgccaggaaa aaagttctcg tgatcggctg tcccgggtcg ggaaaaagca cgtctgcccg
     3001 gcggcgggac aggactgagc tgccgctcta tcctatcgat cttctccggc acaggccgga
     3061 caaaacggcc gtttccgaag tggaattcga cgcggcgctc accgtatttc attcccgcgc
     3121 cgagtgcgag gcctatgaga ttccggcggc ggaagcctga cgattccacc ttggaggacc
     3181 gatatgaacg aaacaagcca agccgaccgt ttcgccccgc tgttcgcgga gcttgaccgg
     3241 cggctcgccg gagaatcccc cgtgcgtctc gccattgagg gcgggagcgc gagcggcaaa
     3301 acgacgctcg gcaaaattct cgccgaacgg tatgactgca ctgtgctcca tatggacgac
     3361 ttttttctcc gcgctgagca gcgcaccgcc gcgcggtttg ccgagcccgg cggaaatgtg
     3421 gatcgggaac ggtttctcgc cgaggtgctg ctcccgctcc ggcgcggcga aaaggttttt
     3481 taccggcggt tcgactgcgc ttccatgcag ctgcaaaatg ccgtggagac cgcgccgaaa
     3541 aagctcgtcg ttgccgaggg ggcatactcc atgcaccccg accttgcgcc gtattacgat
     3601 ctttccgtat ttctggacat ctccccggaa ttgcagcgcg agcgcatttc ccgccgcaac
     3661 acaccggaga aagccaaccg cttttttgcc gaatggatcc cgatggagca ggcgtatttc
     3721 gccgcgttcg atattccggc gaagtgcacg ctgcgccttc ccatactgta aagaaagaag
     3781 aggcacctgc aatctcatca tcctcgactg cgccgccgga aagaccggac gggcgttgtg
     3841 ccggcgatcc tcctccacaa atcgggcatg agccgcgaat acattatcac ggattttatg
     3901 aagaccgcat ccaatccgga agcgtatctc gcgcagtttg ccggtgagcc ccccggcatc
     3961 cggctcgacg tcatcacgcc gcacgagcgg tacatggaag agctcctcgc gtggctcacg
     4021 gcgcaggaga tataaggcgc actggcaaca gcgcgttgct tgcctgcgtc ggagccttcg
     4081 ggtataattt ccccaagagg tgatcggaat atggaaacaa aagatgtgat attgcagctt
     4141 cggaaccggg aaaacctgtc gcaggaggag cttgcggaaa agctcttcgt gacgcggcag
     4201 gcggtgnnnn nnnnnnaaaa cggcgaaacg ctccccaaca cggaaacgct caagcgcctg
     4261 tcggcgctct ttgacgtctc catcaatacg cttctcggct cgccgcgccg gaacatctgc
     4321 cagtgctgcg gtatgccgct cgaggacgaa ataaccagcc gcgagccgga cggctcgttc
     4381 aacgaggaat actgcaaatg gtgctattcc ggcggcgagt tcaagtatga gagcgccgag
     4441 cagctcattg atttctgcgt ggagcatatg gcgacggagg catggcccgc ggagcaggta
     4501 cgcgcgcaca tggaagccgt cgtgccgcag ctcaggcact ggaagcacgg ataatgatta
     4561 ttacaaaaaa agcgtatggc cgcggccata cgctttttnn nnnnnnnngg cttcgctttc
     4621 gggcagaggc tcggtttttc cgtcgggcag cgtttccgcc gcttccgcag ggtcaagctc
     4681 gatcacgggc gcctgtcccg cgaggaactc ggcgcgcgat tttccgtgcg ccaccgcaac
     4741 ggtggtgaag aggccggaaa tgccgtccag caggaagccg agcgccgcgg caaacagaag
     4801 cgtatcgttc ttttcaaagg gattcacaag cagcagcaca cccgccgcac agatgcacac
     4861 gcccagcaca agcgccgccg accagccgcc ggcttcgcag cttttgatat ccagcccgtg
     4921 cttgcagtcc atgaagccgt ggtaaagcag cacaacgccc atcacaatga ccgtaaggcg
     4981 catgacctgc tccgcaaggc tctgcgtgag caggatccag acgcccgcca ccgcagcgat
     5041 gatgccggag atgagcagca gctttcgcag cagaccgacg cgctccccgg taaggtacga
     5101 ggcgatctgc aatatgcccg agaggatcat caccgcgccg aagccgtagc tgatccactt
     5161 catggcggtc gccgtccata tcagcatggc aatgccgaga gcaagacaga gaatgctcac
     5221 gccgaacgcg gcaaaagcaa aatgacggaa aaaacgtttc atggcgtctc ctttcaaatg
     5281 accgaataga tcgtggaaac aagctcgtac cgcagcggat agtgcgtcat ggtgccgaga
     5341 gcggaagcgt cggtcctttt catcagcagc acgtcggcct cgcccgcggc agccgcggca
     5401 gcgagaacgg acgcatcgcc ggcatagacc tcggcaatgt atccggtcgc ggtctcaaac
     5461 gccgggagcc acgctgtgag gatgccgctc tccaaaagct ccgtatccgc tgcgacgcgc
     5521 acacggcgcg tctcatccgt ggccgcgggg atctcttcgg aattacgctc tgtagctggg
     5581 accgtataca tcggcgtatc cagcccgtcg gggataaagg tctccgctgt gtccttccct
     5641 gcggaacgaa gccagcggct gagcgccttc gccccgtcgc tcctgccctc gcagaggaca
     5701 tacacggtct cctcccccgc ggtgaagcgc ggcgcggcgg tcggctccgg agttggctcc
     5761 gccgtcggct ccggcgtcgg agcatccgtt gtctccggcg cagcggacgg cggggcggac
     5821 ggctccgccg tatccggcgc tttggggccg cacgccgtca gcagcaggca cgccgcgagg
     5881 aaaagcggca caacatattt cttcatggct tattctcctt atcgcagccg ccggaacagc
     5941 tgccgcagtc tccgccgcag ccgcgcttcc ggtttttcca gccgcgcttc acggcgatca
     6001 ccgcccacac ggcgacgccg agcaaaataa cgatatcccc tatggacata cgatgccttt
     6061 catttcacta aataagcagt gcgatctggt agaccgccgc gccgagtccc caggcgagca
     6121 ggcactgaaa caccacaacg ccgaacgttt tccacaccga gtcaagctcc cgccggatcg
     6181 ccgcgaccgc cgccacgcag ggcgtataga gcagcgtgaa cacaagaaac gccgccgcgc
     6241 tgcgcacgga gaaaagcgtg gagagcgccg cgccgccggt gagcacgccg agcgtgctga
     6301 caacggcttc ctttgccata aagcccgcga tgagcgaggt gcatacgcgc caatcggcaa
     6361 agccgagcgg cgcgaagagc ggcgcaagaa gtccaccgag cgcggcgaga aggctctgcg
     6421 aattgtccga aacggggttg atgcgcgtgt cgaacgtctg caaaaaccag atcacaaggc
     6481 ttgccacgaa aatgacggta aaggcgcgat ggagaaagtc cttcgccttg tcccacatga
     6541 gctggagcgt tgtgcgcacc gagggcatgc ggtagttcgg cagctccatc acaaacggga
     6601 tggggttgcc gcgaaagagc gtttttccga acacgagcgc cgccagaacg ccgataagga
     6661 tgccgaaaac gtacagtccg cccatggcga gcgccttata cttcgggaaa aacgccgcga
     6721 cgaacacgga atagatgggg atcttggcgc tgcagctcat aaacggcgtg agaagcacgg
     6781 tcatgcggcg gtcacgctcg ctcgtgagcg tccgcgttgc catgaccgcc ggaacggtgc
     6841 agccgaagcc gatgagcatc ggcacaacgc tctttccgga gagaccgatg cggcgcagga
     6901 gcttgtccat cacgaacgcg acgcgcgcca tatacccggt gtcctccagc atggagagga
     6961 aaaagaacag caccacgatc accggcaaaa attccaccac attgccgacg ccgccgaaaa
     7021 gcccatcgat cacaagcgag tgtacgaccg gattgatgcc gtaagccgtg agcgccgcgt
     7081 ctgtcactcc tgccaatgcg ttgatcccca tggcaagcag gtcggacaaa aacgcgccga
     7141 tcacttcaaa cgtgagataa aacaccagca gcataatgcc gacaaagatc ggcagggcga
     7201 atatgcggtg ggtaaggacg ttatcgatgg cgcgcgaacg tttccgctcg cggctctcct
     7261 cgggtttttt caccgtttcg gcgcagaccg attcgatgaa ggaataccgc atgtcggcaa
     7321 tggcggcgtt gcggtcgagc cccgtttcgc gctccatttc cacgatgctg tgctcacaca
     7381 gctccagctc gttctcgtcg agcgcgagac gcgcgacgat atcgctattc ccctcgatca
     7441 cgttcatggc cgcccagcgg gcgctcatcc gggcgcgctc ggcgtgatcc tccaccacgt
     7501 gcgtcacggc gtggatgcag cggtggaccg gtccgggcgg gcagaagtcg tatacctccg
     7561 gcttgcgccc cgtggctgcc gtgcggcagg cgagctccgt cagatcggcg atgccttcgc
     7621 cctttgccgc gctgatcggc acgaccggga tgcccagcag ctccgagagc ttcaccgtat
     7681 cgaccgtgcc gccgttggcg cgcacctcgt ccatcatatt cagcgcgagc accatcggca
     7741 cctgcatctc gatcatctgc agcgtgagat aaaggtttcg ctcaatgttc gtggcgtcca
     7801 cgatgttgat gatgccgtcg ggccggccgt caagaatgaa atcacgcgtt acgatctctt
     7861 cggcggaata gggccgcagc gaatagatgc ctggcaggtc aacgacctcg tcgcccttcg
     7921 tgttgcggat gctgccgctt ttgctgtcca ccgtgacgcc gggaaaattg ccgacgtgct
     7981 gattgcttcc ggtgagctga ttgaaaagcg tcgttttgcc gcagttctgg tttccggcaa
     8041 gagcgaatct catgcgagct ccacctccac cttggcggcg tcctccagcc gcagcgaaag
     8101 cgtatacccg cgcagaaata cctccaccgg atcccccatc ggcgcgatgc gcgttacggt
     8161 aatgtgcgtt ttgggaatga ctcccatatc aagcagccgg agccggagag cgccctcgcc
     8221 tccgacgctt cggatgatcc ccgactgtcc cacatgcagt ttatccaacg tcatttcttt
     8281 ttcacccact ttatattgta ttctctattt tacggcatta tcgtcaaaaa tcaatatcca
     8341 atctgccggt tttatcccgc cttacacttt tgctgaaaac gtatattgat ttcctctttc
     8401 agctctgcta atatagtaca caagatacga cgatatccgt attatatatg ctggaggttt
     8461 catcatgtgc gcacaagctc tgccggaagg cgtttaccgc gaagagatcg ttttcacgcc
     8521 ggaggagacg gacagctttg gccgcgtccg gcccgacgcg ctcgcccacc gctttcagga
     8581 gatcgccgcg gtacaccacg cctcgctcgg cgtcgggcag gagattgccg tgacgaaggg
     8641 ctgcttctgg gcgctcgcgc gcacggagat gcgcatttcc cggctgcccg ccgtgggcga
     8701 gcgcgtgttt ctcgacacat gggtcgggcg gcagtcgcac gggctgttct ggcggcacta
     8761 ccggatcgcc ggtgaggacg gcgcggcgct tctctacgcc gtttcgatct gggtcatcat
     8821 ggatatcgag agccgcgcgc tcaccaaaga tcacgcctgg gccgtcgttt cgcgcgtcac
     8881 ggtcgagggg gaactgcccg cggcgttcaa gcccatcccc ttcccccgtg agctgccgga
     8941 gcgcagttcc cgccgcgtga ccgacgccga gaccgacgga aacggccatc tcaacaactg
     9001 tctgtatctc cgctggggac aggatctgct gccggaggaa tttgcacgca cgcatgcgct
     9061 gcgcagcgtc tggatcgaat atcggaaaga attgccgcgc ggcacagaat cggagttgac
     9121 gtattcgctc agcgggagta ctctgtatgt gcggggaacc gcagaaaaca aagaacgatt
     9181 tttgctgagg atggaatatg atgaaacggt ttgaaagcga ttatctggaa ggcgcgcatc
     9241 ccgatgtgct ccgggcactg atcgaaacga acggcgagca gacccccggc tacggagagg
     9301 acccccactg tgaagccgcc cgcgccctgc tgcgcgagcg ctgcgctctg ccggagggcg
     9361 aggtacagtt tctcgtcggc ggcacacagg ccaaccgcac cgtgatcgcc tcgatcctgc
     9421 gcccacatca gggcgttgtc gcggcggaca ccggccacat cgccgcgcac gagaccggcg
     9481 cgatcgaaaa ctccggccac aaggtgctga ccgtaccgaa cacggacggc aagctcgccg
     9541 ccgcgcaggt tgaggagctc tgccgcgcgc attatgccga cgcgagcttt gagcacacgg
     9601 tgcagccggg catggtgtac atctcgaacc ccacggagtt tggcaccgtg tacacgcgcg
     9661 gcgagctgga agcgctccac gccgtgtgca aagcgcgcca tctcccgctc tttatcgacg
     9721 gcgcgcgtct cggcgccgcg ctctacagcc cggtgtgcga ctatacgctc gcggacatgg
     9781 cgcatatgtg cgaagtgttc acgatcggcg gcacgaaaat gggccttctc ttcggcgagg
     9841 cggcagtgtt ctccgcgccg gagctggcta aggatttccg ttacttcatc aagcagaacg
     9901 gcggcatgct ggccaagggc cgtcttctcg gcgtgcagtt ttacgcagcc ttcaaggatg
     9961 ggctctatga ccgcatcggc gcggaggaag tgcgtcttgc gcttcgcctg cgcgacggct
    10021 tccgcgcccg cggctgggcc ttcctggtgg agtctccctc caaccagcag ttccccattc
    10081 tgccggacgc gctcattgag cggctcaagc cggagttctc gtgcagccat acgcagtctt
    10141 tccccaacgg catgagcgcc gtgcgcttct gcacgagctg ggcgacgacc gacgccgaca
    10201 tcgacgcgct gcttgacgcc gttcccatca acgcctgaac cgcataagta ttgccaaagc
    10261 cgccccgccc ggatcgattt ttcgggcggg gcagctgtcc gttagaaaag ttgcgcgata
    10321 gttggtggcg caaccggcag cggggtgtta tatnnnnnnn nnngtgttat attgacggca
    10381 aaaaggaggt catttccatg aaaatttcca ttcttatggc tgtcgtgtcg cttctgatcc
    10441 tcgccgttgg cgtttatgtg ctcgcgcttc tcatccgtgc gctgcgaaag tacatccgct
    10501 ccgagcccat gcggaaggag aaggcagcga gcgcccgatc gctcggtgag gtattgaagc
    10561 gccgccggac ggagtgcaaa atgacgcagg agtttgtggc ggaggcgctg ggcgtcagcc
    10621 ggcaggcggt ctccaagtgg gagagcggcg catccgatcc aagcacggcg aacctcattg
    10681 cgcttgcaaa gctcttcggc gcaacgccgg aggagctatt gaaagaaatt cagtaacagc
    10741 agaagagcgt ccttccatac ggctttgccg catggtggac gctcttttct cttttatgcg
    10801 gcaatattct tccctttgcg catttcgcgc ctgtaaaacg ggatgagcag cgccgtggcg
    10861 ccgatccagg caagcggcga agagaagcac acgcccgtgt agccaagcac acgcgcaagt
    10921 cccagcgcgc cgctgatgcg catcaccagc tcgacaacgc aggcgagaaa cggcgcggtg
    10981 ctgttgccca tgctctgcac cgtggatcgg taaacgacga gaagcccgag cacgacgtaa
    11041 aacggcgcga caacggtgag atacccgccg gcataggaga tgacctcctc ggtcggcgcg
    11101 tcgatgaaga gcgacacgac cgcgcgccgc gcgagcagca tcgccgcgca catgaggacg
    11161 ttgacgctct cgagctggat gagcgaggct cggacgccct cgcggatgcg gtcgaaccgg
    11221 tgcgcgccat agttctgcgc aacatagccc gaaatggcga tctggtacgc cgtattgacg
    11281 agcaccgaaa gctgatcgac ctttgtcgcg gcggtatagc ccgccaccgc cgccgttccg
    11341 agcttgttga cgcacgcctg cacaacgagc tgtccgatgc acatcacgga catctgaaag
    11401 cccatgagaa acccgacgcg cagatggcgc gccgcggcga ccctgctccc cgcgaagtcc
    11461 gcccggcggg tgcggagcac cggatacttc cgcagtccga cgatcgtgca gaggaccgcc
    11521 gagaggagct ggctgagcac tgtcgcccat gccgcgcccg caacgcccat atggaacggg
    11581 acgatgaaga gaatgtccag cccgatgttg agcagcgacg agagcacgag gaagatgagc
    11641 ggcgtgcggc tgtcgccgag cgcacggagc atataggaaa tgatattata aaaaaccgtc
    11701 gcgccggtgc cgagcagtat cacgaagaga taggcatagg cgtcggcgaa gatgtcctcc
    11761 ggcgtctgca tccagccgag cactggccgg gcgagcagac atacgagcgg cgtgagcagc
    11821 agcgtaaata ccgccgagag cgtccacgac accgcagcgc tccggcgcat gccggtctca
    11881 tcccctgcgc cgcagcactg gcccaggcag ataccgaagc cgccggtcag cccctgcacg
    11941 aagcagagga cgaaatagct gatgatgcct gtggagccga cggcggcgag cgcgtccgcg
    12001 ccgagcgttt ttccgacgat gaccgtgtcg gcgagcgtat agaaaagctg gaagaggttc
    12061 ccgccgagca ccggcagcgc ataccgcagc agcacgctga acggacggcc ttccgtcaac
    12121 tgtctttcca tgcagaaaac tccttttaat tttcgcgcag atactatact ccacgccgcc
    12181 gaaaataaaa agcggcataa tccacaagga tgcaaaaccg gctttcgccg catttgtacg
    12241 ggaaagcagg ttttgcattt ttctatgtaa acgccctgcg gcggtcgttg ttggtctgcc
    12301 gctttcggct cctccgcggt ggacagttac ctcttaagca cagcgccggg tcattcacgc
    12361 tttcccgtgc tgtggagcat ggctgcataa taccggctgt acctgaaacc gaatcgaaaa
    12421 atccggcgaa caaattgtga attcgtggga agaaaaactt ttttcgaccg ggattttttc
    12481 aggcgcattt cagcttggga tatgatatta tacccatcaa gggagggatc tccatgcgca
    12541 ttctcattgc tgaggacgag gtgagcattg cgaaagcact gcagctcatg ctcgaaaaga
    12601 ataaatactc cgtggacgtt gtccacaacg gcacggacgc gctcgactat atctgcgcgg
    12661 gcgagtacga cgcgctcgtg ctcgatatta tgatgcccgg catggacggc atttccgtgc
    12721 tgacggaggc gcggcggcgc ggcgtaacga ccccggcgct ctttctcacg gccaagagcg
    12781 aggtggaaga ccgcatcgcc gggctggacg ccggagcgga cgactatctg cccaagccct
    12841 tcgccgcggg tgaatttctc gcgcgggtgc gcgcgctcgt gcggcgcagc agcgcctatg
    12901 cgccggacgt gctctccttc ggcggcacaa cgctcgacgc gagcgcctat atcctccgcg
    12961 ccgccggggg cgaggtgcgt ttgaataaca aagaatttca gctcatggag ctgtttctgc
    13021 gccacccgcg ccagatcttc tccgcggagc ggctcatgga caagatctgg gggcttgaca
    13081 gcgacgcgga gatcgacgtt gtatggacgt acatcggctt tctgcgcaaa aagctgcggg
    13141 agatcggctc gaacgcagag atcaaaacca tccgcggcgc ggggtacacg ctggaggaaa
    13201 cgtcatgctg aaaaaaatgc agcgccgctt catcggcgcg gcaatggcgg cgttcactgc
    13261 cgtgatcctc gtgttgttct gcgtcgttaa cgctggaaac taccggagcg ttgcctcgca
    13321 gcaggacgac acgctcgcgc ggctgctcgc tcagagcgag cgcagcacga aaaatgacag
    13381 cgaaacaccg ccggagaagc ccgacgcgga aaccggcgct tcgcacccgg acggtgtttc
    13441 agtcccccgt atcgacggcg ggaagaattt ctcgccggag gtaccgtaca tgttccggta
    13501 tttctccgtg cggtacgatg ctggcggcac gcccgtgcag gtcgatcagg atttcatcgc
    13561 atccatctcg cgcgatgaag ccgaggagta tgccgccgat gtgctcacgc tttcccggac
    13621 gcgcggctat tataagggct accgctatct tgtcgctggg acagacggcg gtacgagcgt
    13681 tattttcctc aactccgagc gcgagctgca ctcgatccgc gcgcttttct ggatcaccgc
    13741 cgtcgtggcg ggcgggtgtc tggtcattgt atttttgctc gtgctcctct tttcgcggcg
    13801 cgccattgcg ccgtatatgc gcaatcttgt catgcagaag cagtttatca cgaacgccag
    13861 ccacgagctg aaaacgccgc tgaccgccat ttcgacgagc gcggacgtgc tcgccatgga
    13921 gtacgaggac gacgagtggg tgaaaaacat ccagtcgcag tccgtccggc tctcgcggct
    13981 catctccgat cttgtgacgc tctcacggct cgacgaggaa aacccgttcc cggagcgaac
    14041 agaattttcg ctcagcgatg ccgtgtggga gaccgccgag ccgtttaccg cgctcgcccg
    14101 ctcccgcggc cggagctatt cgcagaacat tgaggacggc ctcaccatga cgagcgatcg
    14161 cgccgcagtg cagcagatgg tttccattct gctggataac gccaataaat acacccctga
    14221 ggacggcagc atttcccttt cggcgcgccg cgccgggcgg aaaaacgaga tcaccgtttc
    14281 caatacctgc gagggcgcgg agagtatcga cgtgacgcgg ctcttcgacc ggttttaccg
    14341 cgcggacgaa tcgcattcga cgcgcatcgg cggcacgggc atcggtcttt cgatcgcgcg
    14401 tgccaccgcc gaagcc
//
LOCUS       DAWAAE010000038        13905 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_1972_length_13905_cov_3.236968, whole genome shotgun sequence.
ACCESSION   DAWAAE010000038 DAWAAE010000000
VERSION     DAWAAE010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13905)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_13905_cov_3.236968"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4889..4898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9199..9208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11822..11831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2488 a         4393 c         4270 g         2724 t
ORIGIN      
        1 gccaaccatc tgttagacta cactgagctg gacgacagac atgccattgt ggaggtcgcc
       61 gtcccggaac gatgggtcgg tcagaccgtc ggcgagctga acattcgccg caaatacggg
      121 atcaacctgc tgggcttaaa gaaggacgga aaaaccaatg tatccgtcac tccggacacc
      181 catctcacaa aggatgttac gattctcgcc cttggtgaat atcaaacgct gaaaaagtgc
      241 tttgctcttt aaagcaaatt gttggatgca gtcatcccgt aaggataact gcatccaaca
      301 ttcattttta aacctttatt ttttcattgc cagccgcaca aaaaaccgca cgagcggcgc
      361 ggtgcagcat acgccgatca gcacgcccat cccccacggg aaggtcggcg caagcgccgt
      421 ctgccagacg gcaatcgcgg tgaacatcac cgctgtcgaa aggcgtgtat cggcaatgag
      481 atcgcgcacg cgccggtaca ccagcgcacg gttgaacacc gtcacgctcg tgccgacatt
      541 ccatgtctgc gggaagaaac tcacggcgag catcaaagcg tcgcacagaa cgccgacgac
      601 cggcatgatc cagagcgttc cccggctgcc gtagccgtcg atctcgccgt aaaaattata
      661 gtgagacggt atctccgccg ggagttgcgc ataccgcacg gcgacataca cgatcccggc
      721 aagcagaatg gcgggggcgg caaagaacgc gatgcgctga tagagcgttt cccggcagcc
      781 gcagaaagcg tcgatctgag ctcttttcat aactgttttc ccactttcag atatccagct
      841 ccggcggctt atccagctcg tcggaaacat actttccgtt tttcttccgc tggcggacgc
      901 attcggtgag cagatactcg atctgtccgt ttaccgagcg gaaatcgtcc tccgcccacg
      961 cagcgatggc cgcatacagc ttcggggaca ggcgcagcgg cacctgcttc ttcccgcttt
     1021 ccgtttccgc catatcagta cagacttccc gaattcacga cgggctgcgc gtcgcggttg
     1081 ccgcagagca caacgagaag gtttgatacc atcgccgcct tgcgctcgtc gtcgagcgcg
     1141 acgacgttgt tctcgctcag gcgctcgagc gccatttcca ccatgccgac cgcgccgtcc
     1201 acgatcattt tgcgcgcgtc gatgatggcg gacgcctgct ggcgctgcag catgaccgcc
     1261 gcgatctcgg tcgcgtaggc aagataggtg atgcgcgctt cgatgatctc gatcccggcg
     1321 gcgtccacgc gctgctggat ctccttgcgg atgcgctcgg ctacgatctc gctcgagccg
     1381 cgcagactcc cttcgtccgc cacgccgtcg ccggtcgtat cgacgttcgg cgccacgtcg
     1441 taggggtaga tgcgcacgat gttgcgcagt gcgctgtcgc actggagaga aaggtattcc
     1501 ttgtagttat caacgtcaaa gacggccttc gccgtgtcca ccacgcgcca catgaccgcg
     1561 atgccgatct ccacggggtt gccgagacag tcgttgatct tctggcggga gttgttgagc
     1621 gtcatgatct tgagagagat cttcttattc ccggcgactt cgatcgagcc gctcgtctgt
     1681 ccggcagcga tcacgatgcc gttcttggcg ccgtccacgt cgccgctctg gctgagcttt
     1741 gtgttcgccg cggggttgac cgccgagcag aacggattca cgtaataaaa gccctcgccc
     1801 ttgagcgtgc cgatatactt accgaagagc gtgagaacga gcgcttcctg cggcttgagg
     1861 atacgcagac cgcaaagcgc gatccagccg agagaaacgt acagaacgcc gagaacaacg
     1921 aagaaaacat ggccgcgcac cgcgccgaaa atgatggcga ggaccgcggc ggcaagcagc
     1981 gccagatacc caagcagcac cgccatgccg tacttccggc cggtaatgat cttttcctgc
     2041 atgatacttc cctccataat tctatttgat atcatattga tatcatgcct tctgcgcttt
     2101 gtcaagaaaa ttctgtaaat caagaccccc ggcacggccg ggacatctcc tgacggaaaa
     2161 acccccgacg cgatcttgca tatcgcatcg ggggtcgttg tccgttgggg aatcaggggg
     2221 cgcggaaaac catgctgcca tccttatgca aagggaggct tatggtcatt cctccagcag
     2281 ccgcggcatg agcatacagg cttcctttgt gaacacgccg gttttttcgg cctccgcttc
     2341 ggtgaagcgg tgatcgttcc cggcgttcac accgctgcgg tagagcgcat acggcaccgg
     2401 cgtcggcgtg tgggtgagct tggcgagcgg cgtcgggtgg tcgggcatgc agagcgcgga
     2461 gaacgcttcc ccgctttccc gcagatattc aagcaccggg aggatgatct tctcgtcgat
     2521 ctgctcgatg gaccagatct tctcctttgc ctcgccgtga tgaccgcact cgtccggcgc
     2581 ttccacgtgg atgtacagaa aatcaaatcc gctccgcagc gcctcgaccg ccgcgcggcc
     2641 cttggcggca aagtccgtgt gatagtttcc ggtgatgccc gccacgggga tgacctccag
     2701 ccccgcgcac acgccgatgc cctgcacgag atcgaccgcg gagatgaccc cgccgcgcgc
     2761 aatgccgaat ttctcccgga acggcgtgag cgccgggcgg cgcccctcgc cccagaacca
     2821 gacgctgttc gccggatgga gcccggcttc aatgcgcttt tgattcaccg gatgcttacg
     2881 gagaatttcg cgcgagcgct ccatcagcga gagcaggagc gtgcggttct cccctttcgg
     2941 aagatggccc gccacgggct ttccggtgat atcgtgcggc ggcgtgagct ccgcgcccga
     3001 ggcggcggcg cgcagcacga gacagtggcg gtagcgaaag ccagcgtgca gctcgcagcc
     3061 cgccgcagca tagagcggcg cgatcgcggc gatgagctcg tgcgcttcct cgttggagat
     3121 atcgtcggcg ctgtagtcga tcatcacggc gttctcgatg ttgtcggcgt ccgagagcgt
     3181 cacgagattg cagcggtacg tcacatcctc cggcgcgaga gggatgccca tgctggccgc
     3241 ttcaagcggc gagcggccgg aataatagat cttcggattg tagccgaaaa cggagaggtt
     3301 cgccgtgtcg ctgcccgcgg gcatgccggg cgggacggtg cgcgtgaggc caaattcccc
     3361 ctgcgacgcg agaaagtcca tgcccgggtg ccgggcaagc tcgagcggcg tatgaccgcc
     3421 gagcggggcg aacggctcgt cggccatacc gtcgccgaga aataccacat gcttcatatc
     3481 atttcaccag ccagtttttc acggattcgc gcatttcgtc tttattgacc acggccttga
     3541 aacgctcctc ccgcgctcgc agcgcgtcga gcggcgcggg gatgggcgtt tcggcaaacg
     3601 cggagagcgc gtcgagcgcg gcaaagccgt cttccggcac accttcgccg agcgaagaga
     3661 gcatcgccgc ggcaaacttg aagggcgagg ccgtggagag cgcgatcgtg gggcgcactt
     3721 ccccgctctc gcgccggtac tgctccgccg cggagagccc ggcggcggtg tgcggatcgg
     3781 ccagatagcc ttttttctcc cacacggcgc ggatgacgcg cgccgtttcc tcctcggacg
     3841 cgaaaaaggc ggcaaagccc tcgtccgcaa ggagtcggag aagctcttcg ccgacggcgt
     3901 actttccctc gccgcgaaga gcgcgcatcc attcgctcac ccgctctcca tcctgcgcgg
     3961 caagatagag aagccgctcg agattggagg atacgagaat atccatcgag ggggacatcg
     4021 tgcgcaggaa tggtcggttt ttatcgtata cgcccgtgcg caggaagtcc gtcagcacgt
     4081 tgttgctgtt cgaggcgcac aacagctttc cgaccgggag accggagcgc ttggcaaagt
     4141 atccggcgag aatatcgccg aagttgccgg tcggcacgga gaagtccacg cgctcgcccg
     4201 gagcgacggc gcccgcgcgc agaagctggg cataggcggc aaagtaatac gcgatctgcg
     4261 gcgcgagacg gccccagttg atggagttcg cgcttgagag cacgacgccc gccctgtcga
     4321 gctcggcggc gagcggggcg tcgtcaaaat tgccgcgcac ggcgagcacg aggacgttgt
     4381 tcccgccctg cgtcgccatc tgcgcgcgct ggatgtccga aacgccgccg tcgggataaa
     4441 ataccgcgat cttcgtgccc ggcacatccg cgaagccttc gagcgcggcc ttgccggtgt
     4501 cgccgctcgt ggcgacgagg atggcgacag tgtgatcgtc cgcgcacttt ctcacgctcg
     4561 cggcgagaag gtacggcagg atctgcagcg ccacgtcctt aaaggcgcac gtcgggccgt
     4621 ggaacagctc catcgtccag agtttatccc ccagcgcgtg cagcggcacg acggcgtcat
     4681 cgtcaaaact tgcataggct tttttcgtat aatccagcag ctcttcgcgc gaaaagccgg
     4741 gcaggaaccg ctcgagcacc gcggcggaaa gctcggcata cggcagcgcg taaagcgctt
     4801 caagctcgct tgcggaaaaa accggcagct cgtccggcac atacagtccg ccgtccggcc
     4861 cccggttttc ccgcaggaca cgctgggcnn nnnnnnnnag tccccgccgg atggcctccg
     4921 ccgccgtgac cgccggcgat ttccctctgg tactgataaa tttcacgggt aagtccccct
     4981 gttgcgtaca tttgttcttc cgtcgttata ctatacgcca ttctgcggcg aaaagcaacc
     5041 gcaaaaaacg aaaagaaatt cgcaatttct gtggaacatt tcagcagttc tgtgatatac
     5101 tgtttggtat tctgcctcag gaggtttgtc cctttgcttt tggaagtttt gcgcgagacc
     5161 gtgatcgaca gtttgaagct gctgccgttt ctctttgcgg cgtatctcgc gatcgaattt
     5221 attgaagacc gcgccgagga aaagaccgtg gcgctcgtcc accgcgcggg acgctggggc
     5281 ccggtgctcg gcgcggcgct cggcgtgatc ccccagtgcg gcttttccgc cgctaccgcc
     5341 aatctctatg ccggcggcat catcacgcgc ggaacgctgc tcgcggtgtt tctctccacc
     5401 tcggacgaga tgctgccgat cctcctttcg agcagcgccc cggtctcgct cattttgaag
     5461 ctccttggct ataaagcgct cgccggtatc cttctcggcg tgaccgtcga taccgtggct
     5521 tcaaagctcg gctacgagcg gaagaaatcc atccacgaga tgtgcgagca ggagcactgc
     5581 cactgcgagg acggcatctt cagatccgcg ctgcgccata cgctcaagat cttcgcgttt
     5641 ctcttcgttg tgacgttcgt tctgaatctt ctggtggagg ctgtcgggcc ggaaacgctc
     5701 ggcgcgttcg tgctcaaccg ccccgtgatc ggcgagctgc tcggcggcat tatcggcttc
     5761 atcccgaact gcgcggccag cgttgtgctc acgacgctct atctcgaagg cgcgatgtcc
     5821 ggcggcgcga tgctctcggg ccttcttgtc ggctcgggcg tcgggcttct cgtgctgctg
     5881 cgcatgaacc gcgactggcg cgataatctc gtaacggcgg ggcttctcta cgccggcggc
     5941 gtcatcttcg gtctgctcgc ggatgtgctg cacattgtct gatcttcata tacggcaaag
     6001 cgccggaagg acaaaaatcc ttccggcgct ttactttttc gtcttatgat tttacggcgg
     6061 tgttgatcga gtcgcggaat acgacgaagc gattgaagag aaattccgta acggcgttga
     6121 cgagcatcgt cggaacgagg atcgcaaatt cattccagcc gtgctgcgcg agcgcctcgc
     6181 cccaccagat agaaagcggc gtgaacacaa gatagtaccc gagcaccttc gccatggcga
     6241 tgggcacgtt gttcgcgctg cggaacgtgt atttccggtt cgcggtgaag ttgaagatca
     6301 ccgagagcgc cagcgccacg aagtacgagg ggccgtactt cagctccgtg accgccgcgc
     6361 ccgggaacag ctttaaaaag accggcaggc ggtcaaaatg cgccgcggtg ttgagcagcg
     6421 tgaagctcac gaactggatg atgcccgcgc ccgcggtgca catcaggtat ttgagcgcca
     6481 tgagcagatt atcacgcttg gatagcgtcg gcttttcgtt tttcggcgtt tccggcatgg
     6541 ataataccgc ctcccttctg tcctcacgtt tataccagag atcggcgcgg cgcgcaagag
     6601 aaattcttcc cgcggtacga agtttcactc gatcgtaccg ccgccgagga cggtgtcgcc
     6661 gtcatagaat accgccgcct gccccggcgt cacggcgcgc tgcggcgcgt cgaacacgat
     6721 gcgtccgcgg ccgttttcct ccggatacac cgtcgcggcc tgctcccgcg cgcggtagcg
     6781 gatcttcacc gcgcagcgca ccggctctcg cggcatctcg ccggagatcc agttcacgtc
     6841 cgataccgcc gcttcgctcc ggtacagatc cttctcttcg ccgaggacga cctcgtttct
     6901 ctccggatcg atccggcaga cgtacagccg ctcgccgtgc gaaatgccga gaccgcggcg
     6961 ctggccgacc gtgtagtgaa tgatcccctt atgctcgccg agaacgcgcc cgtccgtcga
     7021 gacgaatgcg ccgggaacgc tcttctcccc cgtcaggcgc ccgatggcgg cggcatagtc
     7081 gccgtccggg gcaaagcaga tgtcctggct gtcccttttg cgcgcgttgc cgaagccctg
     7141 cgtctcggcg atggcgcgcg tctcactctt gcgcatggcc cccagcggga agagcgtgtg
     7201 cgcaagctcc cgctgcgtca tggcatagag cacatagctc tggtccttcg tctcgtcgag
     7261 cgccttttgg agaatgtatc tctcccccgc acgggcgatg cgcgcatagt ggccggtgac
     7321 gatcttttcg cagccgagct cctcggcgcg gcggaagagc gcgtcgaact tcatgcgccg
     7381 gttgcactcg atgcaggggt tcggcgtgcg ggcgcggcgg taagcctcgc agaacggcag
     7441 catgacctgc tcgcggaagc cctcggaaaa attgaaaacg cagtagcgca tcccgagccg
     7501 gtaggcaacg gcgcgggcgt cctccacatc gtcgagcgag cagcaggtgt gtctttcgga
     7561 tatcccggcg tcggtgttcg tatagagctt catcgtgcag ccgatgcact cgtacccctc
     7621 gcgctgcgcg agatacgccg cgacggagga atcaacgccg ccgctcatgg cgatgagcgc
     7681 cctcacgtct tgttctccga agcgtcctcg atgaggacgc gcatcgtccc gccgcagacc
     7741 atgccctcgt ccgcggccac gtcgttcgtc atgtcgatct cgataacgca gctctcgccc
     7801 gtgccgatga tcttccgcgc gcgctgcatc gccgcagcct cgctgcatcc gccgccaatc
     7861 gtgccgcagc ccgtgccgag cttgttgacg gccatgagcg cgccggattt caccggcgtg
     7921 gagcccgtgg acgtgagcac gagcagcagc gccttcggct cagcgttctc cgcaagatac
     7981 cggatggcgg aaagatcgga attcgtctgc tcgagcagcg tgccgggata ctctgtctcc
     8041 ggcagagcgc ggcggtgctc cacgagctgc gcgcagatgg aaacggcgat ctccgcgggc
     8101 gtcaccgcgc cgatggcaag gccgatgggc gcgtggatgg cggcaagctt ttccgcgtcg
     8161 tacccctcat cccgcagcag cctcatcagc cccgccacgc ggcggcgcga gccgatcatg
     8221 ccgagatacg acggcatcgc gccggagagg atctgccgca gacagtcggc gtcccagcgg
     8281 tggccgcgcg tgatgacgca gacatagtcg ctctctcgga gtttgagcgc cgcgatcgcc
     8341 gcggcgaagg cgtcgcacac ggtgtgcgcc gcttccggga agcgcgacgc ggcggcaaag
     8401 tccggccggt cgtccaccac ggtcacgtca aaatccagca tagccgccat gcggcagagc
     8461 ggctgcgcga tatggccgcc gccgagcagg atgagccgct cgcgcggccg gaacagtctt
     8521 gtatactcca cgccgtccac cgtccggtgc agcacggcgc tgcgcccggc ttcaagttct
     8581 tcgagtatcg ggcgaaattc ttcctgtatc atacgatcgc accttctttt gctttttgtt
     8641 ttacctacta taacactatg tttttatttt ttcaagaaat atcggaacat tccgaacgcg
     8701 gctccgtcct ataatcagac gccgacgcgc acggcgtttc aaaaatcaaa aaacaaaaag
     8761 gagtttatcc gataccatga aaaagattct ttctctcgta cttgctctcg cccttgtgct
     8821 cgcgcttgcc gcgtgcggcc cggtggcgac gctccctgcg gatggggaga ttccgaaccc
     8881 gtggcaggac tttgacacga tgaccaacgc cgaggccgcc atcggctacg gcatgaccgc
     8941 gccggagacc gtcgccggac tcgcgccgag ttccttccgc gttctggaca gtgacggtgc
     9001 caaaattctg gaagtctgct acggcggaga gagcgatgac cgcgcttccc tgcgcaaagg
     9061 caccggctcg gcggatatca gcggcgattc caccgattac gccaaaaccg agaccgtgac
     9121 ggtcggcgat atcaccatga cggaaaaggg cgacggtgag aacgtattct ctgccgtctg
     9181 gacgaaaggc gattattcnn nnnnnnnncg ccttcttctc caccgtcggc gtgagcgccg
     9241 acacgctcgc cggtctgatt gccgggatcg agtaaaaaca gcaataccat aaaaccgtac
     9301 agcgccggaa gtgacatcct tccggcgctg ttctgttgac acggcatacc cgcgcgaatt
     9361 ataattaacc atgtatcccc tatataaaaa tgtatgaccg gaggataaat tcggtgaact
     9421 tgtctgcgtg gaaagaccgg ctggccgccg gaaagtacga cgccccgctc gcagcgctct
     9481 acggcgcggc ggacgcgccg cggtcacgct acgcgcggct ttgtgaagaa tatctccgga
     9541 cgttcggcga cgcgccggac gcgcttttga tcagcgcgcc ggggcgcacg gagcttgccg
     9601 ggaaccacac cgaccaccag ctcggcatcg tgctctgcgc cgcggtcacg ctggacgccg
     9661 ccgctgccgc cgcgagcgcg gcggacaacg ccgtggagat cgtctccgac ggcttcggcc
     9721 gggtgcgcgt atcgctcgat gatctcgcgc cgcgcgaaag tgagaccggc acgaccgccg
     9781 cgctcatccg cggcgtcgcg gcgggatttg ccgcaggagg ccttcgcatc ggcggctggc
     9841 gcgcgtgcat ccactcgctc gtccccgccg gaggcgggct ttcgtcctcg gcggcgtttg
     9901 agatactcac cggcgcgatc ttctccgcgc tgtataacga agggcgcgtg gacgcgctca
     9961 cgctcgcgcg catcggtcag agcgccgagc gaaactattt cggcaagccg agcggactga
    10021 tggatcaggc cgcgagcgca ttcggcggca tcacgaagat cgattttgcc ggcggcgacc
    10081 gtccggacgt tacgcgcatt gcgtttgatt tccgcgcgca cgggtatatt ctctgcgccg
    10141 tgaacacaca ctcgcgccac gacgatctca cgccggacta tgccgccatc ccgcgcgaca
    10201 tgaccgccgt tgccagagcg ttcgggaagg acgttctgcg gcaggtggac gccgcggcgt
    10261 tcgccgcgcc ggagatgcgc gagcggctcg cgcgcgatat atcccctgtc gccgcagacc
    10321 gcgccgagca cttttttgcc gaggacgcgc gcgtggagcg catggccgcg gcgctgctgg
    10381 agggcgatat gccggagtat atccggaaca tgaacgcctc gggcgcatcg tcccgcacgc
    10441 tgctgcgcaa tgttgtcccc gcgctgcacc cggagcggac ggaaatggcc gcggcgctcg
    10501 accgcgctgc ggcgctgctc gaaggcaggg gcgcatggcg catccatggc ggcgggtttg
    10561 ccgggtgcat ccaatgcctt gtaccggcgg cagactatga agccttccgc gccgcgatgg
    10621 atagctgcta tggcgagggc gcgtgctttg agctgcgcat aagagggtgc ggcgcgtacc
    10681 gtatcccgga agaggaataa ccatgtacaa cgaccttttt tcgattggcg gtctcacggt
    10741 gcacggctac gggctctgca tcggtcttgg catcgccgcg gcgctgctgc tcatctggcg
    10801 gcgcgccgag gagcggaagt ttgacgtgca atccgtcaac accctgattt tgctcattct
    10861 cgctgccggc tttgccggag cgaagctctt tttcgtgttt gcgcattgga gcgaattttg
    10921 cgccgatccg ctcggcgcgc tcggctcgga aggctttgtc gtctacggcg gcattgtgtg
    10981 cggtctcggc gctgcgtatc tctactgccg gaagcgctct ctcccctttc tgcgctgggt
    11041 ggactgcttt attccctgcg tggcgctcgc gcagggcttc gggcgcatcg ggtgctttct
    11101 cgccgggtgc tgcttcggaa aaccgacgga gagctttctc ggcgtcactt tcccggcggg
    11161 cagtatggct ccggcgggca tcccgctctg gccggtgcag ctcttttcgg cagcgggcga
    11221 tttcctgctt gccggagcgc tgctgcttct ggaaaaaaag cgccgcggcg acgggctgct
    11281 cgccggagcg tatcttcttt tgtacagcat cggccggttt ctgatcgagt ttctgcgcgc
    11341 cgatccgcgc ggcgcggtcg cagcgctttc gacatcgcag ttcatcgcgc tcttcaccgc
    11401 cgcggcggga gccgccattc tcattttgcg aaagagaggg acaccgcatg gataacaccg
    11461 tcagcgccgg gcgcaaaagc acaacgctcg gcggcagagt atgggcctgc cttctgatct
    11521 tcggctttgt cggccagatc gcgtggatgg tggagaatgt ctatttctcc acatacattc
    11581 agaaaaacat caccgccgcg ggctgggcga caagcgctac cgtgtccgca agcgccattg
    11641 ccgcggcgct cgccacgttc ttcagcgccg catggtccga ccgtgtggga cgccgccgct
    11701 cgttcgtctg ctgggggtac atcctctggg gcgcgacgac cgccgcgttc gcgcttttcg
    11761 gcaacgggca ggtcgcgctg ccggtgggtc ttgcggtgac gatgttcgtc atcatggact
    11821 gnnnnnnnnn natcatgtcc gccatcggct cggcggcgaa cgacgcggcg ttttccgcct
    11881 gggtcacgga cgtgacggac gtcaccaacc gcggcaccgt cgatatcgtt ttgagcatca
    11941 tgccgatcct cgcgctcgtt gtgatcttcg cggggtttga cgccatgacc gtgaacggca
    12001 actggacggg attttatctc gtgctcggcg cggtcacatc cgttgccggc attctggggc
    12061 tctggctctt taaggacagt ccgaacctcc gcccgacgaa gggcgagagc tatctgcgcg
    12121 aggtcatctt cgcgttccgg tcggagaaca tccgcgcgaa caaaatgatc tacgtctgct
    12181 tcttcggcat gatgttctcc gggctcgcca tgcagatgtg gcagccgtac atgatctcgc
    12241 tcgtggaggt cacgctcggg atcgaaaatt acatcgtgcc catcgccatc gtcgtgctcg
    12301 cctcggctgt gctgtctgtc gccgccggaa aagtcatgga ccggttcggc aaggaaaaat
    12361 tctattaccc cgtcgcgctc atgcaggtgc tcggcggtgt gatcgcctat ctcatcaagt
    12421 ttgtcggaca cgccatgccg cttctctgcg tcggcgggac gctcatcatg gcgggcaatc
    12481 ttatgatggc cggtctcttc acggcctcgg cgcgcgacta tacgcccgag ggccgcgccg
    12541 gcgcagtgca gagcgtcaag atggtcatct acatcgttct gcccatggtg ctcgcaagca
    12601 ttctcgatcc tgtcatcatc cgcgccgtcg cgctcgagcc gacggcggag atcctcgcca
    12661 aatacccgag ctacgcgggg agctatctct acccgtatga gctgttcctc ggcgcggcgg
    12721 tcgccgcggt gttcattttc ctgcccgcct ccgccgtccg gcgggacgcc aagcgtctgc
    12781 gcgcgcagaa gctcaaggag atccgcgaat gacgatgctg acaaagtggg gcgcggcact
    12841 cgaccgcagc tgccccttac cggaatatcc ccgcccgcag ctgcggcggg agagctttta
    12901 taatctcaat ggcgtatggg actatgccgt cacgccgtcc ggtgacgagc cggaggtctg
    12961 ggacggcgag atcgtcgtgc cgttctcgcc ggaagcgccg ctctccggcg ctgcgcgcgg
    13021 cccggcaaag aacgaatacc tccattaccg ccgcggcttc acgctgccgg agggatttca
    13081 aaaatcgcgc gtgctgctgc atttcggcgc ggtggatcag atcgcagagg tgttcgtaaa
    13141 cggcgtttcc gccgcgaagc actccggcgg ctactggccg ttttccgcag acatcacgag
    13201 tcttctgcgc gagggggaga acattctccg cgtaacggtg cgcgacaatg ccgacgatcc
    13261 cacgttcgcc tacggcaagc agcgctataa gcgcggcggc atgtggtaca ccgcgcagag
    13321 cggcatctgg cagacggtct ggctcgagag cgtgccggag gtctatgtaa agagtctgcg
    13381 catcacgccg gagtttgacg aggcgcgcgt gcgcatcgag ctggagaccg cgctcgagag
    13441 cagcgccgcc atcacgtgcg acgtgcgcgc tgcggacggc agctttgtcg ccgggagctg
    13501 ggcaaagggc ggcgtgtgcg tcattccgct ccccggcttc cgcgcctgga cgccggacga
    13561 tccctatctc tacacgctgg acatcacctg cggcgaggat cgcgtggaga gctattttgc
    13621 catgcgcaaa ttctcgtatg tggagctgga cggccacaag gtgtttggat taaacaacgc
    13681 ccccctcttc cacaacggtc tgctcgatca gggatactac cccgacgggc ttctcacgcc
    13741 gccctcggac gaggcgatgg taaacgacat tgagaccatg aaacgctgcg gcttcaacat
    13801 gctgcgcaag cacataaaga tcgagccgct gcgctggtac tatcactgcg accggctcgg
    13861 tatgatcgta tggcaggaca tggtgtccgg cgggcagccg tacag
//
LOCUS       DAWAAE010000039        13648 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2003_length_13648_cov_3.225189, whole genome shotgun sequence.
ACCESSION   DAWAAE010000039 DAWAAE010000000
VERSION     DAWAAE010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13648)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2003_length_13648_cov_3.225189"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4197..4290
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8259..8268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8866..8875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2347 a         4040 c         4193 g         2954 t
ORIGIN      
        1 gccgcgagag gcgcggcgat gatcgcgtag atcatcgctg cgtccttcgc ggtgatatgg
       61 ccgtcgccgt ccatatccgc aaggataagc tccgcttcgg tgagttcacc cgcgccgatg
      121 gaataattgt acacaagctc ggcgtcagtc acattgaccg tgccgtcggc gttcacgtcg
      181 cccgcgagag gctgctttgc gccctgccac gcgaggatcg ggtaaccgcc gttgatgttt
      241 tcgctgtcct cggcgaagct gccgccgagc gtggcggcca gggccttgag ctcatccgcg
      301 gtcttggcgg tgccgaggcc ggtgatgccg aacttcgggc tcgcgccgga aacggtgcct
      361 tccagatagt ggctgttgac atagttcgct tccactttta tcggggacag gccgccgaca
      421 taggaggcgt tgccgcccga accgatgacg gtggaggtgc tgtagcagtt ggtgatcgtc
      481 gcgccgtcgg tgttgctgaa gtacgagccg cagataccgc cgagatagca ctcgttcgcg
      541 gagatggtgc ggatcgtgcc ggtggcgtag cagtcggtga tggccgaacc gccggccgtg
      601 tagcctgcga tgccgccgcc gtgcttactg cccgtgacgt tcacggagct ccagctcttc
      661 gtaacggagg tgccgctctc catgtagccc gcgatgccgc ctgcctgtcc ataggacttg
      721 ttggtcgtcg tcacattgcc ggtgatgccg agcttcgtga cggaagcgcc ggttttcaca
      781 taaccgaaca ggccgaccgg gtagctcgcg ctgttgatat agaggttgcg gatggtgtgg
      841 ccgtcgccgt tgaagctgcc gtagaactga ttcagcggcg tccagtcgta gcccgcgaga
      901 tcgatgtccg cggtgagcac cgccgaggca ttgctgccgt tcgcgccgaa ccacgcgagc
      961 tcggaggccg taccgatctg gtaaacgccg tcggcgtcct tcgcaggctc ggtcttcgcc
     1021 gtgccgtccc aggcgttttc gcccgccggg agaaacgccg cggtcgtgac ggtgaggatg
     1081 ccgtctttga tcttctccgc atcggcgctg ccaagcgaga aggcgccgcg cgtgacaaca
     1141 tagccgtcct tggtaagaac ataggtgtaa tcgccgtagc cgagcgtgta gcggttgttc
     1201 tcattggtct cgacggcctt gccgtccgag ccggtcagcg tgatcttgac gccctcgacc
     1261 gcattgccgg aggcatcggg aacgacgagc ttcgtatcgt aggtcacctt gcggtgcacg
     1321 gtgacggctg cgtccgcgta gtggttcata tagtaggtaa cggtcacagc gttgaagttc
     1381 gtgccgacgc cggcgtcggt catattgtaa aggaacgcaa acgggttggc cgcggagtac
     1441 atgctgccga aggtatagcc gttggtgaag ctgtacgctg ccgtctccgc gccctcggcg
     1501 aactccacgt ccgtggggat ggtcacggta acggaggcgt tgtccatctt ctgatactgg
     1561 ccgagcgtgc cctcgaactt cgtttcaccg gcgtagtacg tcggcttgaa ggtcgtcggg
     1621 ttgaagatgc cggagatctt gttcaccgcg cggaacatgc cgctgaaggt gagcttgacc
     1681 tgatcgccgg gcatgatgtc ttcctccggg tgggagacgt tctcggcgga gacggtgacc
     1741 ttcgccacgc gcacgagacg gtagctcacg ccggtatcgt cggtcatctt gatgatgacg
     1801 gtgccgccca tgccgttgcc gatctcgccg aacttagcaa gaggcacggt gtagcgtccg
     1861 tccccgcccg cttcgatggc ggcgaactcg gtgcggatgc ggttcatggc gttgtccgcg
     1921 gtgacgaagg cgtattcaac ggtcacatcg ccggtggcgg tgacggcaaa gtcgagcgcc
     1981 gggtcggcct cgttcgcgcc gtagaaccag gtgtcgtagt tgtagtccca atccatggag
     2041 cgggtcgtcg tggtgcccgg agccatgttg aagtccacat ccgcatccgc ggtgccgtgg
     2101 gccacgcccg tgccgccgac aacgatgacg ccgatgcgct gcggctgcgt cgcaggatag
     2161 taaccgccgt gcgtgccgta gctgccgtgg ttcacgtcaa cgctgtcata gtaaacggtg
     2221 atgatggagt cttccgtgcc gggggtaacg tccgcccagt tcgcggtggt gtttccgccg
     2281 ttcacggtct ggatcgaggc gtcgccggtc tcaaggttcc agtggaagtc cggctcgacc
     2341 atgatgtttc cggcgtcgga gttgatgagc tgccagtgac ggtaggcgcg cacgcgcgtc
     2401 gtgccggaaa tggcctcata gccggaggga tcgaggttca cctgcaggtc ggcctcgtcg
     2461 cgtttggccg tctgcgaacc gagccggctg aaatcgtggg agacacggtt cgtcgcggcg
     2521 ttctcatcga aggagaagct cgcgtccgtg ctctcgctca ggctcgggag ccagccggcc
     2581 tgcgtgacgt gggtatcgtc gctcagacgc cacgtgtagt tgccgttgcc tttggaaatg
     2641 gtgtactgcg catacatcgt gccgtcgtcg ttgagcttcc agctgccgtc gggctcaact
     2701 tcggtggtgt tgaagttgtt ccactggaag tacaggccga acgccgcgtt ctgcggcacg
     2761 gtgacggtca ggacgattgc cgtgttgatc gtgagggact ttgtaccggc ggagtagccg
     2821 gcgcggaacg tctggttgat ggcttgcgtg aacatatagc cgtcgatatc gggataggca
     2881 tacgcgttgt agaggcaggc gttgcccgcg gcgtagagca ccgtcgggta gtaggcgtag
     2941 ctgctcttgg tgtaaggcgt gcccattgca gcggtcgtgc ccataattgg gcagtccagt
     3001 ttgacgtggt attcgtccgc cgtgaagtac gtgttgtccg tcttctttga ggacgtgtag
     3061 aacgagttgc actggagata gatgctcgtg ccgccgcccg cgccgccgtc aacgtttttg
     3121 tcggtcggca ggtcaagcgt cataccgccg agcgcgatgt cgtatgcgcc ggtatcggcg
     3181 ttcttggcga acgcgcggta agcatagcgg cccgcggtga ggctggcata gaacatctgg
     3241 taattgttaa cgagcttgcc ctgcgcggga tcatactcgg tctcgccctc gggcagcgtg
     3301 ctcgtgaagt cgctgtagag gaacacgttc gtaatctcgt cgccgaggac ctcgttgcct
     3361 tcctcgtcag ttgtgacatt gtagagcttg ataacggggt aattgccggt atagtccttg
     3421 ccgacaaaga acgagaagtt cagcttcggc gcttcctcta cggtgagagt gagcgtccag
     3481 gtgtccttgg agaagtgctt gccgtcgtgg gaggcgtaga agcggtagat gtacgttccg
     3541 gcttcgtttt ccgtgatctg gatggtcgtc atgccgaaga gggcctcggg gaaggtctgc
     3601 tttgcgctcc aggtcgcgcc accgtcggcg gagcgctcgt agtagtagtt cttgtaggac
     3661 agcgtctggc cttctgcggg atcgaagatc ttgttggcct gcagatcgct gaggacatag
     3721 gccttgccga tctgcgcctt gtcgctggta ttggccggat aatccgcttt gcgcgtcggc
     3781 acggcgatct ccgcgagctg cttgccgcag gtatcgcact tgccgtcgcc gttcgcgtcc
     3841 gcatgctcga ccacggtctg gccgctgagc acgggggccg ggcagctgtc aacgtagctc
     3901 ttgccgagct tggaggcacc gagcccctct tcggcggtga aggcttccgc gccgctctcc
     3961 acgccggaga ccgccgccgt gccttcgaga taataggcgt tcgcggcaat gacttcgact
     4021 ttgttgctcg tgccgctctt cgtgcctccg acaagagcgc ccataagagc ttcatcggct
     4081 ttgtcgccgc tgatcttgcc ggtgctgtag cagccggtca tggtgagcgt ttgcgtccca
     4141 taggaatcac cgaagcagcc gacaagaccg ccgacatagt gtttttccgc cgcggtnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgacaagacc gccgacgccc tcaaacgtgc
     4321 tcttgtagcc gcgcagatgg acgtggccgc tgttccagca gttcgtcagc gttacgccgc
     4381 cgatgccggt attgccgacg atgccgccga cctgcccctg atagccggtg atgtccactt
     4441 cgttggagca gccgatgaag tcggtctcct gtgcgctgcc gacaagaccg ccgtgggaga
     4501 aaatatcatc gtttgtcgca gagccggtga gagtcacata gttgtggcag ttctcgacag
     4561 tggaacggcg gtgcgtggaa acgccgcacg tgcgtccggc aagaccgccg accatgcggg
     4621 tcacggccat cgccgtgccg tagaccgaga ggttcttgat cgtcacgccg tcgcactcgc
     4681 cgaagaggcc gatgtaatag gtgttgccgt tcgtatagtt cgaggcgttg cccttgagat
     4741 acaaaccggt gatcttgtgg ctctggccat caaaagtgcc gctgtatatt ctgttattcg
     4801 cgtgatcgcc gatcggcgtc cacgggttcg ccgagctgca gatgccgctg agatcgatgt
     4861 cgtccgtcag acgggcgttg agcgcggcgt tatccttcga ggcgttgacc tcgttggcaa
     4921 accactggag ctgcgcggcg ttcgagatct cataatagcc gtcttccgcg agagcgggct
     4981 tgctcgggtc ggtgacggta acggtgcatt gggccgtgta gccgccgtcc gccgtggtga
     5041 cggtaatggt cgccgtaccg gtgcccacgc cggtaacaac acccttcgtg ctgacggtgg
     5101 caacgtccgt gttactgctc ttccagctaa cacgggggtt gtccgcgttc tgcggctcca
     5161 cggtcgcggt aagcgtcgcc tcttcgcggc ggatgagcga aagctcgctc ttatccagcg
     5221 tcacgcccgt gacggcaacc gcgccgccgg gggcggtgtt atcgccatac acaaagccga
     5281 agcagttgaa gtagtcgtaa ttgctctgct cttcggcagc gaagtaccgg ctgttctcaa
     5341 caagatacgg gaccgggagc ttcagcgtct tgccgccgga ggcgttgtcc agcagttcaa
     5401 agccaggaca gtcgtagccg gcaacagcgc ctgtggtatc aagcagagcg caaaagctca
     5461 ggaacgtata tttaaagtcc gccgggtaat tgacgtatac atagtccgtg ccttccggaa
     5521 cggtcaccgt gtagagcttc ttatcgcccg gaaccttgaa atgctctaca taaatcgaac
     5581 tctcttcgat gttgtagata tactgcgtgc cctctgcgtc gagcgtgatg gaggcaaaat
     5641 cgaagttctt ataatgctcc gccgggtcga agggcttcga gctgctgcca acggtgacct
     5701 tcgccgtcgc gggagcaagg cgcgcgtcgg tcgagcagca attcgtgtcc atcgccatca
     5761 ggtaatacgt accagcgttt ttaaacgtat aggcgtagct gctgccgccg tcctgctcgg
     5821 gcaaaacagc gtccagaagc gtcatatttt cgtcgtaaac gccgacatcc atgccggtca
     5881 cggcggtgaa cgtatcgctg ccgccgtcgg agacggattt ggtgtcatac ttgagcgtgg
     5941 aaaaactgac cgtgccgccc ggcgcaacgc tgtaggcatc ctgatcgaag cgcgcgaacg
     6001 cgccgccgtc cgaccagaag ctccagttgc tgtacatggc gacgtcgatc gtgtcgccgt
     6061 ccgagaggag gatgtagtcc gccgtcgaac cccagcccgc gctcatcaga ggatagatat
     6121 ggttgcggta gtacatgaga ttctggtcgt gaccccagaa attctccatg tacatggacg
     6181 tggcgctgcc ggagagactg agagcagcgc ccgtatcgtc atacgcctta tcgccgctta
     6241 tccacgagac accggcgccg ccggcgtggt cgacgatctt cgtgctgccg ctcgtgacct
     6301 ccgccggatt cagcccgaga tagtatacgc cgatcatgta aagatacaga tgcagcgccg
     6361 tcggacgctc gacgatcttc tcatccacat acttgcccga gccgttttcc gtgtggtaac
     6421 ggtaatacgc gccaagatcc tggttggcca gatcgaagta cgggacggtc acctcaagat
     6481 gcgagatggg cgtgccgtcc ttgccctgaa caggcatgcc gtcgctcgag atcgtcacgt
     6541 acaccgtgac cgaatcggcg cgcgactcat cgtagcggta ctggctctcg tcgcccttcg
     6601 gggcctccgt gacggtaatc gtgagctgca cgctggccgt ctcgccgttg gagcacgtcg
     6661 ccgtgacagt cggagtatac gtgcccatgg cggagtttgt gaacgagaga cactgttgcc
     6721 catcgacctc ggcgatttgg gtatgctcgc cgtaattgcc gttgtccagc gtataggtca
     6781 gcgtatggcc ttcgctgtcg gtgaaatact gcgcaagctg aaccgtaagg ttctctccct
     6841 gctttgctgt aaaggttttg tccgcacccg tcgccgtgag gcatccgtcc gccgcgagcg
     6901 ccgataccgg cagcagcgag aacaccatca gcagcgcgag aagcagtgca agacctttct
     6961 tacccgtttg cattggtttt ttcccttcct ttccgatgat tccttgaact taccttttat
     7021 atttatatca agcgcagaat ctccgaggca tcttccgccg tgatcctccc gtcaccgttc
     7081 agatcggcct tcagccaggc ttcggcgttc ttcgtctcga agcgctgctg tccgcagctg
     7141 atggaataga tcctctgcgc gtcattgatt tccgtaacgc cggagccgtt cacgtccccg
     7201 tcgtagcaga ccgtatcgct gccgccgctg caccgcacgc gggaaacgcg cgccgccgca
     7261 tcgttggccg ttgtcagcgc gcaccacgcg ccgtagggtt ccgagcagag catttccgcg
     7321 ccgtccaatg tccagcggca gcgcgtgccg ggctcggccg cgaccttcac gagatacacc
     7381 gcaccgtcct cgctcccggc gtattccgtc acggatatct ccggcagggt ttcaggcgtt
     7441 tcgtatgccg catagtacgc cgcggggcgg taatagttat ccatgccgaa atcctcggca
     7501 taggagaccg tgacgccgcc gcgcatgcag tggcacatga gcacgcgccc atcgtccgca
     7561 aagcccgtca cgatgccgac gtggtcggtt tccgccgtgc cctcgccgcc gaagacgaga
     7621 tcgccgggcc gcagctcttc gcgcgttatc gcttcgcacg caagacgctg cgcgctcgtc
     7681 ccctcgccga tggtgggaag cgcctcgacc gtcccggcgg cgttgagcag cacccacgtc
     7741 acaaagccgg agcagtccag accgtcggtg aatatttcgc cgttctcgtt ctgctgcggg
     7801 aagccccaga aggggctgcg gcccaggtcg ttatacttgc cgccccagag atattcgatc
     7861 cttccctgct gcgcgagcgc gagacggacc acatcctcgc ggagcgtata taccctctcc
     7921 cccgcgccgg actcgaccca gccgtacccg cgccagagag agaggagctt gtcccactca
     7981 tccccggtgc acagcgcctg atagcgctcc tgctcttccg cggtcacgag aagcgccgat
     8041 gcgctgcgcg agagcaatat cttaaaagaa agcgcatact tcgtttttat aacgttcggc
     8101 tcgccggtct catcattgag cgtgccgtcg gtcatgtccg tttccatgag cgtacaggaa
     8161 acggcgctca tagcgccgaa caccgtgcgg agcacggaga gctcctttgc cgtgagatcg
     8221 gcctccgtca gctcgtccgc cgttttgccg gtcttcgcnn nnnnnnnncg gcgatgaacg
     8281 gcagcgcgtc catgttgtcg gtgagcgtac cgtttccgta ggcgagcgtt atctcttcga
     8341 tctctttctc ggcgctctgt tccgcttcgg tcagcacgag cgtctgcaca aggccgtcaa
     8401 ggagagcgtc cagtgcctgc gggaacggga gtccctcggt aaggacggga aaatactcgc
     8461 tcggctccac ggcggtctcg cgcgtttcga gagcttcaag ttccggttcg ggcgttgccg
     8521 tcggttccgg ggattccgtc ggttcgggcg tttccgtcgg ttcgggcgtt tccgccggtt
     8581 ccggcgtttc cgtcggctcc ggggcttccg tcggttccgg ggattccgcc gcttccgggg
     8641 cttccgtcgg ttccggggtt acctccggtt cgggcatttt ctccggcccg gacgcttctt
     8701 cggtttccgg gacgtcctcc gccgcggggg tctccttcgg tgcggatgtc ttctccggtt
     8761 tggggaacgc ttccggcctc ggcgttatct ccggcgcagg ttccgcagcc gccgtcgccg
     8821 ccggttcatc ggcgtcggcc gccagcgcag ccggcgagac tgccgnnnnn nnnnnagact
     8881 gccgcgagca tcgccgccga aagaagcagc gccagtattt tttgtaaatt tcttctcata
     8941 cgatcattcc ttaaagaaga acactcttct ggagttccgc gtggacgggc tggccgtttt
     9001 tcaccgtgat cacgatcttg ccgtaaccca gctcgcggat caggcgcagg atctcctcgt
     9061 cctgttccgt ctgctccggc ggaacggttt tcgtcatttc gtccggcatc gctgtcgtca
     9121 ttccgatctt catctccttt tttccgagcg ccgttttgac aaaaaacagc cctccatcga
     9181 taagacagag ggctgtttgt gcgcaaaagg aacgccaacg gacaccctat aggccggacg
     9241 cagttcagtt aactgaactg cccccatagg gagggtcgtt ggcggatctg caatcagtaa
     9301 aaacatacgg agagctcgcc cgctgtcttg ggttccggac tttggtaatt ctcctggctc
     9361 gcgctgcgtg aaaagttctt cttttcctcg tccggaatct accttctccc gaggctgact
     9421 gccccgaaat ggcggacttt cgtccctgcg gctctccgcc gcttcgatcc ggactcgcgc
     9481 atacagtacc gtgcgtgcag gggattcgca cccctttcct ttttcaccgc cgcctgactt
     9541 tcaaaaaagc ctttacagca aacggctacc tgtccgatag atccatattc aattctaaat
     9601 aaattatacc gggtgtcttg gaaaagtcaa ggtttggcac cggcattttt tggcaaaatt
     9661 ctggcttttt cctttcttaa tatatttatt ttaaattgct tccatatcat tttaaaaaag
     9721 atatgccccc ggctttttcc gggggcatac ggctttattc ccattgcttc cacacgtccg
     9781 ggcagtagcc ggcggtggcc tgttttccgt tgcggacaac gggcgttttc aaaagcgtgg
     9841 ggtcttccag aatgcgctcg agcttatcgt ccgcgcgggc aagatagcgc agcagcgccg
     9901 cgtccgggtg cttttcgtcg atgagatcct caagccccac ggcgtttttc acgctttgca
     9961 gctcgccgcg gctgatgccg tagcggttga gatcgatgta ctggtatttg atgcggcgct
    10021 ccttgaagta ccgctccgcc tttttcgtgt caaagcactt gcttttgccg aagatctgta
    10081 tgttcacagc gcttccccca ggaaccggtg gaggttgtgg aagaagatat cctgcacgag
    10141 cgcttcgctg aagcggtgcg cgagcatctg ctcatacagc tcttccatgc tctccacgcc
    10201 ggtgatgccc tccggcagcg actcgatgcc gtcaaaatcg gtgccgagac agagattttc
    10261 gcgcgcgtcc atgcagagga aatggtcgat gtgcgcgagc acggcgtcca tattccgccc
    10321 gaggccgaga aaggcggggc agagattcag cccgatgacg ccctcgttgt ggacgatggc
    10381 gctgatctgc gcgtcggtca gattgcgcgg atggtcgcac acggacttcg cgttcgagtg
    10441 gctgcacagc acagggcgct tcgcaatgcg catcacgtcc cagaacgttt tttccgaggt
    10501 gtgggagaga tcgaccgcga cgcccttttc ccagcaggtg ttcacgaagc ggacgccgtc
    10561 agcggtcagg cccgcgccgg tatccattgc cgaaccgcac aggcggttat ccttgttcca
    10621 gcagagcgtc accatgcgca cgccacggcg gtagccgtcg agcagccccg cgacgctgca
    10681 ccggaggaga ttggcgccct cgaccgagag aaacacggcg accatgtcgt ccttcgcggc
    10741 ggcgtcagcc tccgcgacgc tcgtgcagag cgcggcgaga tcgcggtttt ccttcagctc
    10801 acgctcaacg gtgagcatga tgcggcggaa cgccgtgggg ccgtcaaagc ccggcggcgc
    10861 ccagatcgcc atgacctgcg cggcaggggc gtagtggtgc agacgcagaa gatcgatgtg
    10921 gttccggctg ctctgacgca gggaattgaa cttcgtcaga gtgtcgcagt gagcgtcgaa
    10981 atacgggatg cagtacggca gagccatggc gatagtctcc ttttcttttt atttgatgtc
    11041 tgatcccaac cgctgcggga ttccttacag tgcctccctt gtgtaaaggg aggtggctcg
    11101 gcgtcagccg agacggaggg attgtaaact gcggtttctg caaaacgaca atccctcagt
    11161 cagcttcgct gacagctccc tttgcacaag ggagcctttc agacttccat gataatcggc
    11221 aaaatcatcg ggctgcggcg ggtctttttg aagagataac cggaaaggcc ggacttaacg
    11281 cggcttttga tgcttgccca gtcggtgatg ccgttatcct cgcagtaggc gatgctctcc
    11341 atcgccacgc ggcgcagctc gtcgatgagc ttgtcgtttt ccttttcata gacaaagccg
    11401 cggctgatga tgtccgggcc ggagatgagc tgcgcgttct ccgccgagag cgtcataacg
    11461 acgacgacca tgccctcgtc ggcaaggagc ttgcggtcgc gcagcaccac ggtgccgacg
    11521 tcgccgacac ccatgccgtc cacgagcacg cggccggacg ggacagtcgc gccgagcttg
    11581 gcggatttgg cggtcagctc aatgactctg ccgttctcgc cgatgatgat gttccggcgg
    11641 tcgtgaccca tacccacgat catatcgccg ctcttgtgga gcatgcggcg ctcgccgtgc
    11701 acggggatga agaacttcgg ctttacgagc gcgtacatca ttttgagctc ttcctggcag
    11761 gcgtggcccg atacgtgcag atcggtgccg cgctcataga tgacctccgc gcccttggcg
    11821 aagagctcgt caataacgcg ggaaatcgtc gtctcgttgc cgggaatggc gctcgcggag
    11881 atgatgacgc ggtcacccga gccgatgtcc acctgcttat gctcggaaaa cgccatgcgg
    11941 tagagcgcgg acatgctctc gccctggctg ccggtggaaa ggatgacgag cttgttgcgc
    12001 ggcacggagc cgatgcgctt catgtccaca agaatgccgt ccggtacctt catatagccg
    12061 agctccacac agagcttcac cacgttttcc atgctccgcc ccgtgatgcc gaccttgcgc
    12121 ttgtacttga cggcgagatc gatgatctgg cggatgcggt ccacgttcga ggcgaacgtc
    12181 gttacgatga tgcgcttgtc gcagtccttg aagagctggt cgaagcggtc gtgcaccttg
    12241 ctttcggaga gcgagtggcc ggggcgctcc acgttcgtgg agtccatgag cagcgcgagc
    12301 acgccctcgc gccccagctc gccgaagcgc gtgagatcgg tcatctcgcc ctgcaggggc
    12361 gtgacgtcga tcttgaagtc gccggtctgg acaacggtgc cgatcggcgt tttgatcgca
    12421 aacgccacgg aatcggcgat cgagtggttc acgtggatga actccacctt gaagcacccg
    12481 gcggagatcg tctcgcccgc ctcaacggtg acgatcttcg ttttatcgag caggccgcgc
    12541 tcctgcagct tgagctgcac gagccccgcg gtcaggcgcg tcgtatagat gggcgggttg
    12601 atccggtcca tcacatacgg cagcgcgccg atgtggtcct cgtggccgtg cgtgagaaat
    12661 acgccgcgga tgcggctctt gttcttcaca agatagctca cgtcgggaat gacaacatcc
    12721 acgccgtaca tatcgtcgtc cgggaagccc atgccgcagt ccacgactat gatgtcccgg
    12781 ccgtactcgt acgccgtgag gttcttgccg atctcgttga gaccgccaag gggaatgatc
    12841 ttcagttttt gtgccataaa ataatagtta ctcctgttag atagatttag atatgtatcc
    12901 ggacgggccg cgcccttacc ggattatgtt cgcagttggg gaggtttata cctccgttac
    12961 atttcgcggc ggccttcgag cgctttgagc atggtggcgt cgtccgcgta ttcgagattt
    13021 gatccgaccg gcacgccgaa gccgaggcgc gtgatgcgga tgccgaaggg cttcaaaagg
    13081 cgcgagaggt acatcgccgt ggcgtcgcct tcggtgtcgg ggttcgtggc gaggatgacc
    13141 tcctccaccc cgccctgcgc cacgcgctcg agaagctcgc ggatgcgcag atcgtccggc
    13201 ccgacgcggt tcatcggcga gagagcgccg tggagcacat ggtatacgcc gtggtattcc
    13261 cggctgcgct cgatgctgag cacatcgcgc ggctcggata cgacgcagat cgtcgttttg
    13321 tcccggctgt cggacgcgca caccgggcag agctcgccgt ccgtcaggtt ctggcacacc
    13381 gggcaggtgt ggacgctctt cttcgcttcg agcacagcgt tggcaaagcg gtccgcttcc
    13441 tcctgcggga gcgagagcac atgaaacgcc agccgctgcg cgcttttgcg cccgatgccg
    13501 gggagcgagg cgaagccgtc gatgagattt tccatagccg ccggaaagaa cgatgccata
    13561 atactatatt acactccttg aaaaatcagg cgttagcgtc gattgacaag ggcacacgcg
    13621 gttctgccgg gcggaaatgc cctcatac
//
LOCUS       DAWAAE010000040        13624 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2010_length_13624_cov_3.268406, whole genome shotgun sequence.
ACCESSION   DAWAAE010000040 DAWAAE010000000
VERSION     DAWAAE010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_13624_cov_3.268406"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1803..1812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5226..5236
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6601..6610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12484..12493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2692 a         4177 c         4100 g         2614 t
ORIGIN      
        1 gcacagcggc tgaatttcaa aataatcgta gaacgctgcg agccggtcaa gctcagcatc
       61 gctccggttg cgcacgacgg cgtcgaaaat ttcgcccgcc tcgcacgccg agccgataag
      121 aatgccctcg cggtactgca tcagaaggct cttcgggatg atggggcttt tcttgaagtg
      181 ctccagatgc gatttggaga tgagctcgta gagatttttc agtcctgcct tgttctgcac
      241 aagcagcgtg atgtgacgcg ggcggcgggc gtcgtttttg cgcagctccg ccacgagcgc
      301 tccggcatcc gagagcgttt tcgcgccctt gtccgcgagg cgcggacgga gggttgtgaa
      361 gatgcgggcg cacacggcgg catcgtcgct cgcgcggtgg tggttaaagt ccggcagacc
      421 cagcgcgttg gagatgatat cgagcttgaa ttttttgagc tgcttcaaaa gcgcctgcgc
      481 gatggggagc gtatcgatgt atacggggtc aaacgcaagg ccgctgcgct ttgccgcggc
      541 ggtgatgaag ccgatatcaa aggcggcatt gtgcgccacg agcggaagat cgccaacaaa
      601 gtccagaaac gcgcgcacgg cctccgcctc gcccggcgcg ccgtacacgt cgcggtcggt
      661 gatgccggtg agctgcacga tctccggcgg gatgggcatg ccgggatcga cgaacgtttg
      721 aaagctcttc gtcacaacgc cattttccac gcgcaccgcg ccgatctcgg tgatgcggtg
      781 aatgtggctg tccaggccgg tcgtttcgat atcaaagccg acaaaatcgg tatcaagcgg
      841 gagatcgctc tccccgtgga cggcgagaag gtcgtccaca tcgttgatat agtacccctc
      901 cacgccgtaa atgaccttga tgccgcactt ggcccccgcg tgccagaact ccgggaacgc
      961 ctgcgcgacg ccgtggtccg tcacggcgat ggcgcggtgg ccccagcggg ccgccgtttt
     1021 caccgcggcg gtcgggtcgg tgagcgcgtc gagcgtggag tagcgcgtgt ggagatggag
     1081 ctccacgcgc ttgtcccctt cggcggtgtc ctgccgctgc ggggcggttt ccttcgcaat
     1141 ggcggcgaca tcgatcgcca tgtcgttatc gtagcggttg aagatcacgt cgccgcgcac
     1201 cgtgagatag tcgccgacgc ggatcttatc gaggatcgac tgatcgtcgc cctggcggat
     1261 gtacttggaa acgtggatgc tgccggtata gtccgtcata tcgaagctca gcaccgcagc
     1321 gttcgttttc tgtattttcc ggctgtccgc ggcaaagacc ttgcccgtca cgctcaccgt
     1381 gccggactgc agcgtaagct cggagatatc cgttctatgg ttgtccttcg gcagcttgcc
     1441 gaagaggacc tcaccgctct ccgctttttt cggcgcgggc tttgcttcct ttttcggcgg
     1501 cgcggccttt ttctccgccg gggccgccgc gggcgtattg ccggtgatct tcacccgcgt
     1561 aaacccgtat tccgccgcaa tgcgccattc gagcatagcg atatcggagg gcgtgccggg
     1621 cgcggcaaag ccggcggcaa tgtccatcgt ccgcttctcg cgcgagacga caacgttttt
     1681 cacctttgcc agctccagcc cgccgcactt atcccgcagg gcggcgcagc agccgaacat
     1741 atccagaaat ttgacttcct gtgccatgaa ttattggttc tctctttcaa ttagttccac
     1801 gannnnnnnn nngtccgacg ctacgcgcgc caccggctcg ccgtgcttga agatgacgcc
     1861 ctcgctcttg ccgccggcga tgccgtaatc ggcgtgggac gcctcgccgg ggccgtttac
     1921 aacgcagccc atcacggcga ccgtgatgtt ccacggctta tcgcgcagcc gcgtctcgac
     1981 cgccttcgcc gtgccgatga gatcgtattc cgtccgcccg cacgaggggc aggacacgaa
     2041 ctgcacgccg gaccgcagcc ccagcgcgcg cagaagctcc ccgcccgcgt gcgcctcttc
     2101 ctcgggcgca tcggtgaggg agatgcggat cgtatcgccg atgccgtcga gcagcagagc
     2161 gccgataccc gcggcggatt tcagaatgcc gcgctccttc gtcccggcct ccgttacgcc
     2221 gatgtgcagc ggataatcgc acagcgtcga gagcaggcgg ttggcctcca cggtcatggc
     2281 aacgtcgctc gccttgacgc tgatgcagat atcctcaaag ccgcagtcct ccagaagctt
     2341 tacatgaccg agcgcgctct cgcagaggga tttcgcgcac agaccgtatt tttcgagcag
     2401 gcccttttcc accgagccgg aattcacgcc gacgcggatg gggacgcccc gctcgcggca
     2461 ggcgtccgcc accatacgca cccgctccgg agagccgatg ttgccggggt tgatgcggat
     2521 cttgtcgaag cccgcctcca ccgcggccag cgcgagccgg taatcaaaat ggatatccgc
     2581 cacgagcggc aggccggtgc cgcgccggat ctccggcagg gcgcgcgcgg cgagctggtt
     2641 gggcacggcg agacgcacga tctcgcagcc cgcggcttcc agccgccgga gctgtgcgag
     2701 cgtggcggcg acatcgtccg tatgcgtgtt cgtcatcgac tgcacgggaa tgggattccc
     2761 gccgccgatg ggcacgccgc ggacacggat ggttttcgtg atctttctgt tcatcggcga
     2821 atgatcctca caatatcgtt gtacatcaaa agcaccatca gcccgagcag gagcagcagg
     2881 ccgatgtaat ggatatatcc ttcaatgcgc gggtcgggct ttttcccggt gatcttctca
     2941 aagagcgcgc tcaaaaggag cgtcaggata tggccgccgt caagcgccgg gagcggcaga
     3001 agattcatca ccgcaaggtt cacggcgatg aacgccacga ggaacacgac gttcgcaatg
     3061 ccctccgatg tggactgcgc gctctgcccg gtctcgttga tgatatccac catgccgacg
     3121 acgccggtca tatccgacat ggacgcctgc ccggtgaaga gctgcacaag tcccgcgcgc
     3181 accatgcgca cgaagtccag acagttgtag aaggagtatt tcagcacatt tccgaccgtg
     3241 ggcgcggcga tcgtgaagtt gacgccgtat tttttcacgg tctgcccgtt cacctcatag
     3301 tccacgggca ccatgtggta gttatccagt tcgatccgct ttccgtcgcg gagtatgacg
     3361 agatcaagat ccccgtcctc gtcggcggag gcgtattgaa tgaagttgcc ggtaaagaag
     3421 atccgctcgc cgttcacgcg gcggatctcg tcgccggctt gcagcgtgtc ctcatacggg
     3481 cagccgtcgg cgaagccggc gatgacgggg ctgttgaagc cgcccgcgcc ggcatagagg
     3541 atgatgacga gcacgagacc gagcaggaaa ttcatcgccg cgcccgcgac gagaatgaga
     3601 aggcggtgcc agacgctctg gttattgaac gctctcggat cgtccgaggc ttcctcctcc
     3661 ccttccatgg cgcagaagcc gccgatggga agggcgcgga gcgaatagac cgtttccccc
     3721 ttctgcttat gcagaagctg cgggcccatg cccatggaaa actccgtcac cttcacgccg
     3781 cacgccttcg ccacaagaaa gtggcccagc tcgtggacaa atatcaggat gccaaatgcg
     3841 agaattgcaa gaacaatgta catactcagc cgatctccct gcggacaagc tcgcgcgctt
     3901 ccgcatccga tgccagaatt tcctcgagcg tgggctgcgc gataaactcc gcgcgctcca
     3961 cagccgaagc caccagacgg tagatatcat tatacccgat ccggtgtccc aaaaacgccc
     4021 ctaccgcgat ctcgttggcc gccgacatga cgcacggcgc cgtgccgcgc gctctcgcgc
     4081 actccatcgc aagacggagg cacggcagct tctccagatc gggatcttca aaatgcatgg
     4141 aaccgagcgc ggcgaaatcc atcggcttcg ccgccgactc gcgccgctcg ggatacgtga
     4201 gcgcgtactg gatgggaagc cccatgtccg gcacgccgag ctgcgcgatg accgtgccgt
     4261 cgagcagctc caccatggag tgaatggcgc tctccgggtg gatgatgact ttgatctgat
     4321 cgggcgtcac ggcgaaaaga tgcatggcct cgataaattc cagaccctta ttcatcatcg
     4381 tggcgctgtc gatgctgatc ttcgcgccca tgctccagtt cggatggcgc acggcgcgtt
     4441 cgggcgtgac gtcgcgcgtc tgctcgagtg tccagccgcg gaacggcccg ccggagccgg
     4501 tgagaaggat gcgccggagc tcgctcttat cgcgtcccgc catggactga aagatcgccg
     4561 aatgttcgga atcgaccggg aggatctccg cgccgcagcg cgccgcctcc gccatgacga
     4621 tctcgcccgc gcacacgagc gtttccttgt ttgcgagcgc gatgcggcgt ttctgatgaa
     4681 tggcggcgag cgtcgggcgg agacccacgc tgccggaaat gccggtcacg acgcagtccg
     4741 ccccttcgac cgtggcggcg gcgacaaggg attcctcccc ttcgccgatc tcgatatccg
     4801 tgtccgaaag gcgcgcccgg agctcctttg ccgcggcgga gtccatcacg gagatatact
     4861 tcgggcggaa ctgccgcgcc tgctcctcga gccggtccac atcccgccgc gccgcgagcg
     4921 cgagcacggg aataccaaga cggccggctg cctgagcagt ctgccgtccg atggagccgg
     4981 tggagcctaa aatggaaatt gctttcacca taaaatgatc catccttatg ccgaaatcgc
     5041 gggcaggagc agcatccaca gctccgtgag cggcgcgaga tagtacatac tgtcaaaccg
     5101 gtcgagcatg ccgccgtgac cggggaggat attgccgtaa tccttgatac cgtactggcg
     5161 cttgaacgcc gaaaatgtca gatcgccgag ctgcgccaca acgctgccga caagaccaaa
     5221 cgccgnnnnn nnnnnnattg gcgctcaggc cgcacgcgcg gacgatgacg ccgtacaaaa
     5281 gcgccatcgc ggctccgcac accgcgccgc cgatgccgcc ggcgacggtc ttatgcggag
     5341 agatggccgg ggcgagctgc ttcttgccga acgcacggcc gcagaagtat gcgcccgcgt
     5401 cgctcccgaa ggcgacgacg aacggcagaa acgcgcgcac gcgcccgacg atcccgtcat
     5461 gcgtgatgcg aacgagcgtg gagttgccat tcagcagctc cggaatggag atcgtcgttt
     5521 caaactgcgc gatgcgaagg agcgtagaga gcatcatcgg cagcacgccg cccgcgagca
     5581 tcgagatggc aaccatttcc atgctgatct tgcgcggcgt ggaaaaacac gccatcatct
     5641 cgcacgagag aacgaagaag aggaacaaaa gaatgatcgc cgccatgcgc agatcgagcc
     5701 cgccgaaaat ggcgatcgcc acggcggaga ggatggagga aatatacagc cgcttcggcg
     5761 cctgcccgat ggcgcagcgc ataagctcaa acgccacaaa gccgcagatg accgcaatga
     5821 tcacgccgaa cagccagatg ggcgcaaaaa acacgcccaa gattagaacg gggatgccca
     5881 cgagcgcagt caatactctt tttttcacgc tttaactcct ccgaaccgac ggtcgcggtg
     5941 ccggtactcc gcaacggctt cgtcaatgtc tttctctgag aaatccggcc agagcacatc
     6001 ggtgaagtaa agctccgagt aagcgctctc ccacatgaga aaattcgata tacgatattc
     6061 cccgcccgtg cggattatga gatcggggtc ggggataccg gcggtgtcga gatactccgc
     6121 catcgccgcc tcggtgatcg tctcgccggg atgctccgcg gcaaagcggt tcacggcgcg
     6181 gacgatctca tcacgtccgc cgtagttcag gcatacgttc gcctgaaagc agccgggcag
     6241 acgcgcagcg attgtgtcgg ttttatcgat caagccctga atggcgggcg aaagggccgt
     6301 cgtatcgccg agaattttca ggcggatccg gtccttttcc atcttttcaa tggattcgtg
     6361 caggtagttt tccaaaaggc ccatgatcgc gccgacttcc tcatccgagc gcttccagtt
     6421 ttccgtggag aaagcataca ccgtgagata gtccatgccg aggtttttgc agtacgttgc
     6481 aatgcgccgg aatgtctccg cgccgacggc atggcccgcc ttgcgcggca gcccgcgctt
     6541 cttcgcccag cggccgttgc cgtccatgat aatggcgatg tggcgggggg gcttctcccc
     6601 nnnnnnnnnn ctcggtgatc tcgccctttt tctgttcctt cttgaacttg tccatcgcgt
     6661 cgcggcggat gttgcgcacg gcgaccttgg cttcctcgcc gtacttgcgc acctgcttgg
     6721 cgagctcctt gcggcgctcc tcggtgagct gcgggaacac aagacggata acgcgcccgt
     6781 cgttctgcgg gttgatgccg agctcggagg tgaggatcgc cttttcgatg agcttgagcg
     6841 tgctgccgtc ccacggctgg atgagcagcg tgcgcgcatc ggggctggaa atggacgcga
     6901 tctggcggat gggggtggga gtgccgtagt aatcgacctc gatctgatcg agcacggcgg
     6961 cgttggcgcg gccggcgcgg acggtgtcga actgggcgga gagaacctct gccgttttga
     7021 tcatgcggtt tttgaattct ttgatttctg ccatcttgtg tatcctcccg gtgttatttt
     7081 ttcgtttttc ttttaatgga ccagcgtgcc gacggcctcg ccgcgggcgg cgcgcaggat
     7141 gttttccggt tcgcgcaggg cgaacacctt gatggggatg cggttgtcca tggcaatgca
     7201 cgtcgccgtg ctgtccatga ccgcgagacg cttttcgagc acttcctcat atgtgagaga
     7261 gtcgaagcga accgcgttgg ggtccttctt cgggtcggcg ctgtaaacgc cgtccacgtt
     7321 cttggcaagc aataccgcgt ccgcgccgat gagcgccgcg cgcagcaccg cgcccgtgtc
     7381 tgtcgtgaag aacgggctgc cgacgccgcc gccgaacacg acgaccttgc cggagcggag
     7441 atattcgtcc gccttttcca cggagaacgg ctcgccgatg tgctggatct ccatggcagt
     7501 gagcaccttg gcctccacgc cctgctgctg cagcacgtcc tgcagcgcca gacagttcat
     7561 gaccgtggcg agcatgccca tatagtccgc tctccagcgg tccatgcgtc cctcgccgtt
     7621 tttcacgccg cgccagaagt tgccgccgcc gacaacgatg ccgacctcaa cgccctgtcg
     7681 ggcgcactgc gcgacaacgc cgcagacctc ggccatgacc ttgaagtcca gccccgttcc
     7741 ggcatcgccg gagagcgctt cgccggagag cttgagcagc acgcgcttgt acgcgatctt
     7801 cttttcgtcc atgggtttct cctccctttg tacggttcag cgatattttt tgagaagctc
     7861 ttccacgtcc gaaaggaagt gtttgagcgt tttgttcggc ttgccgcggc tcgcgcggat
     7921 gagagaaagg agcgattctc cggcggcgac gagcttttga taaagcccgc tcgccttggc
     7981 gcccgaagcg gtctccgcct ttttgaccgg cacgcccttc ggacatacga ggaatttccc
     8041 ctcggcgaga tcgaacaccg tgccgctgta aggcgcgaac gccttatagc cgtgctcggt
     8101 ctccagatag ccggcaaacg cgtccgtcac ggcgtcctcg ccgtggttca cgaacacaag
     8161 cttcggcttt ttctcaaagc ccgcgagcca cgcggtgagc ccgtcgcggt cggcatggcc
     8221 gctcttgccg ggcaggaagc cgatctcgca gttgacctcg atatcctcgc cgaagagctt
     8281 tacatgcttt tcgccgtcat agagcctgcg gccgagcgtt ccctccgcct gatagccgac
     8341 gaagagcacc gtgctctcct tccgccagag attgtgcttg agatggtgac ggatgcgtcc
     8401 cgcctcgcac atgccgctcg ccgagaggat gaccttgggc tcggggtttt cgttgatggc
     8461 cttggagtcc tcgctcgaaa cggagatgcg gatgccgtcc gaccagatgg ggttgatccc
     8521 ggcgtccacg agagcgcggg tctcctcgtc gaagcattcg tggccgcact ggagatagat
     8581 cgccgtcgcc tcgtttgcca tggggctgtc cacatagacg gggaagccgt cgtgaccggt
     8641 gacgagcttt ttctcgcgga tctcgcggat atagtagagc atctcctgcg tgcgtccgac
     8701 agcgaacgac gggatgacga gattgccgcc gcggtcgagc gtgcgctgga tgtaatcggc
     8761 cagcagcttg accggcggga catggcggtc gtgcaggcgg tcgccgtagg tcgattcgat
     8821 cataacatag tccgcgtccg cgaccgtttc cggcacgttg cggatgatgg gctggttaag
     8881 attgccgata tcgccgctga acaccatctt gcgctccacg ccgttctcgg tcataaagac
     8941 ctcgatgcag gcgctgccga gcagatggca cacatcgctg aagcggacgg cgaagtgctc
     9001 gccgacctgc accagctcgt gatagctcac cgggcgcaga tgcgcgatgg cagcctcagc
     9061 gtcgtcgagc gtgtagaccg gctcatatgc cggtccgccg gagcggcggc ttttgcggtt
     9121 gcgccactcg gcctcgctct cctgaatgtg cgcgcagtca cgcagcatga tgcggcagag
     9181 attgcacgtc tcgcgtgtgg cgtaaatggg gccgtcgaac ccgtttttga aaagcagcgg
     9241 cagattgccg gagtggtcga tgtgcgcatg ggtcaaaagg acaaagtcga tctcccccgc
     9301 cgccaccggc agcttctggt tttcaaagat gtccttcccc tgctccatgc cgcagtcaac
     9361 aaggccgcgg ttattcccgc actcgatgag tgtgcagctg cccgtaactt catgggtcgc
     9421 gcccaaaaag gtaactttca tagtgaactc ccgtgtcgcg gcgcttcccc aaccctttgt
     9481 ggaagagaag ccgcctattt tcacggaaaa cattatacac gttccccgcc cgtcgcgcaa
     9541 gacttattgc atcccgttgc cgccatacgg ttttttgtag tccccgcccc gcgcgagcac
     9601 ggcgcattgg ccggtgctcc gctgcagttt ccctttgaca gcacattcgc tgtttgtggg
     9661 gaggacattt tacgaaattt ctgctatact ttctttgagg tgagccgaat ggatcaaata
     9721 aagataggaa aattcatcgc cgccctgcgg aaagaaaagg gcttgacgca ggaaaagctc
     9781 ggagaaaagc tcggcgtgac caacaagacc gtttcccgct gggagaacgg caactacatg
     9841 cccgacgtgg aaatgctgtc gctgctgtcc gaagaattcg gtgtaagcat caacgagctt
     9901 ataagcggag aacggcttgc cgcggaggat ttcaaaaaag ccgccgacag caaccttgtt
     9961 acagcgctca gcagcagtac atttacgctg aaagaaaaga tcgccttttt taggaagaaa
    10021 tggctgcatg aacatatttc gactatcgtc ctgtgcgttg tggcgtggat aggtattata
    10081 atatggaccg cgctgaaact gcgcggcagc aactcatatg cgcttttggg cgcggtcggc
    10141 ggtatgctcg ccattctttt ctatctggtg ctgttctatc tggtgctgta taaccgcatg
    10201 atggcgtatg tggaaaaacg cgcatacccg gcagcgtcgg ataaggaaac cgaaacgaaa
    10261 aacacttgat cacagcatgt tcaaaatcga tgcagcagca aacttcgagc ggctgtatga
    10321 aagaaacccg gctgccgttt ttgaacggca gccgggttcc gttatttgcg ccacgcggcg
    10381 gggatgaaca gcagaagcat cggatagagc tccagacggc caaagagcat ggagaaagcg
    10441 agcagcacct tgccgaaaga cgagaagcat gtatagttgg acatcggtcc gacgagctcc
    10501 ataccggggc cgacgttgtt aaagcagctc gccgcggcgg aaaatgttgt gatgaaatcc
    10561 agcccttcca gcgcgagcag gacaagaagc agcacaaaga gcagcgcgta cacgagcagg
    10621 aacgcggaaa cggagccgca ggtgtcctcg tcgagcggct cgccgtcgat gtggatgcat
    10681 ttgacctgct gcgggtggat ggcgcgctgc acaccccggc gggcaagctt cgcgccgatc
    10741 acgatgcgca cgatcttgat gccgccgccg gtcgaaccgg cgcacgcgcc gacgaacatc
    10801 agcacaacaa gcagcgtttt ggaaagccac ggccaaaggt tgaaatccga gatagcaaag
    10861 cccgaggtgc tcatcacgga cgcggcggtg aatatggcgt ggcggaatgc ctcgccaaag
    10921 ccgctgaaga gcggcagaat gttcacagca acggcgagcg aaacggcggc gagaatgccg
    10981 aggaataccc acatctcctc gctgcgcagc gcctgccgga actttccgat cagcaagaga
    11041 tagtagagcg tgaagtttat ggaaaagagg atcataaaaa cggtgatgac cgtctgcgaa
    11101 taggggctgt acacgctcat tccggcgttg cggatcgaaa agccgcccgt gcccgccgtg
    11161 gcaaacgccg tggtgagcgc atcgaagaac ggcatatcaa aaatgagcag cacgaacatc
    11221 aaaaccgtga ggccgatata gataagatag aggatcgtcg tgctgcggcg catccgcggc
    11281 acgagttttc ccacgttcac gcccggcgat tccgcgcgca gaagatgcag cgcgtcaccg
    11341 ctgtcaccgt tcgccatcgg gctcacggcc agcacgaaca cgagcacgcc cataccgccg
    11401 agccataccg taaagctgcg ccagtacaaa aggctcaccg gcagcgcctc cacatcggcg
    11461 atcacgctcg cgccggtcgt ggtaaagccg gaaacggtct caaagaaggc gtccacaaac
    11521 gacggcaccg cgccgctcag acaaaacggc agcgcgccga acaccgaaag aagcacccac
    11581 gaaagaccga ccgttacaaa gccctcgcgg gcgtaatact cccgccgccg ggcctttatg
    11641 cgccgcagca gcgccgcgaa cacgaggatc gccgccacag tgacggcaaa gccgacagcg
    11701 gcgctgtgct cgtgccgtgc gagcgcgatt gcgagcggcg gtatcataaa tgccgcttcc
    11761 actcccagcg tcaggccgag aatgcggcag atcatggcaa tgttcatatc cgatcacccg
    11821 cgatcggcaa aaatctgccg cagctccgcg atgtgccgct cgcgcggcgc gaccacgatg
    11881 acggtatcgc ccgcctgtaa gctgtcgccg ccgccgggaa agatcacctt tgccccgcgc
    11941 gaaatgcacg cgagcagaat tcccggctga agcgtgacag aggcaaggct ctcgccgagg
    12001 tgcggcacgt ccgccgtgac gagaaattcg agcgcctcaa cgcgcccgcc cagcagactg
    12061 tatacggaaa ggacgctctc ggcatcggtg ctctccatgg cgcggacata gcgcgagatc
    12121 tcctgcgcgc agatgtcctt cgggctgacg gtgctgcgga tgccgcagtt ctggcacacg
    12181 gcggcgtatt ccgtccgatc taccttcggg atcgcctgcg gcacgccctg catccgggcg
    12241 tacatacaga taaacacatt ttcctcgtcc agattcgtga gcgcgacaac ggcgtccatc
    12301 tgcgaaatat tctcgctgaa aaggaaatcc tgccgcgtac cgtcggcgca gaggacggag
    12361 acgtccggca aacgttccgc aagctcccgg cagcgctcgg cgttatgctc gatgagcttc
    12421 acgcgcacgc ccgcttcgcg cagcagctcc gcgagcgctt cgcccagccg tccgccgccg
    12481 acgnnnnnnn nnnatcatca cattcttcgc gtgttttttc cgcagcccca gttcgtccac
    12541 ggtgcgcgaa agggtcgccg ccggcgcggt cagataaacc tcgtccccgg cgcggatctc
    12601 aaaggagcca tccggaatga tcgcctcttc tccgcggcgc acggcgcaca caagcacgcg
    12661 gtggcgcaga atgcgcggca gctccatgag ctttttgccg cagagcttat tatccgcatc
    12721 gacctccatc gccacgatct cggcacggga tttggcaaac gattcccgcc ggagaaaagc
    12781 gggcagctgc agctggcgga agatctcgcg cgcggcataa agatccggcg tgaccgccat
    12841 ggaaaggccc agctcctccc gcaggagctg cacgccgttg aggtagttgc ggctgcggat
    12901 gcgggcgatc gtgtttgcgc agccgatttt tctcgcaagc atgcacgcga gaatgttcgc
    12961 ctcgtcgttc ggcgagacag cgatcagaag atcgctgtcc ggcacgagcg cagcatgctg
    13021 cacgtcgagc gcggcgccgt ttccctgcac ggtcataccg tcgagcgcga gagaaagccg
    13081 gtcgcacacg gcgcggctgc tgtcgatgat cgttacgtcg tgtccttcgg cggaaacctc
    13141 gcgggcgatg gcaaagccga ccttgcctcc gccgacaatc acaattttca taaaagctcc
    13201 ttcattttca aaaagcgctt cgggcgttta tatagtaagc gcagtatagc atctggcaga
    13261 gtcgatggca accgcttccc gtccgccttg acggcgattt tacgttttgc agaagttttt
    13321 ggaaaaaatt taaatttgtt cttgacagaa tgctataacg tttgctacaa tgacaaagcc
    13381 gcgaaaaacg ggagcatagc tcagctggtt agagcacatg ccttacaagc atggggtcac
    13441 aggttcgagt cctgttgttc ccaccatatc ccgtctttca aagcaagcca tttgcctcgg
    13501 tagctcagta ggtagagcag cggactgaaa atccgcgtgt cgccagttca attctggcct
    13561 gaggcaccat ttgcgggcat agctcatttg gcagagcgcc accttgccaa ggtggaggta
    13621 gcga
//
LOCUS       DAWAAE010000041        13584 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2017_length_13584_cov_3.306675, whole genome shotgun sequence.
ACCESSION   DAWAAE010000041 DAWAAE010000000
VERSION     DAWAAE010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2017_length_13584_cov_3.306675"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7494..7503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2681 a         4147 c         4302 g         2444 t
ORIGIN      
        1 gcgccaatcg tcgtgcggac gatctcctca tgcagcttcg tgagctcgcg cccgagcgca
       61 atgtcccggt cgccgagcac atcgtaaaga tcgccgagcg tttccgcgag cttgtgcggc
      121 gcttcgtaaa ggacgatcgt cctgtgctcg tgccgcagcg tttcgagatg ctccttcctt
      181 gacttgttcg atgtggagag aaagccttca aacgtgaacc gctgcgtcgg caggccggac
      241 atggcgagcg ccgtgatgac cgcgctcggc cccggggccg ccgataccgt gatgccgtgc
      301 tccccgcaca gccgcaccag ctcctcgccg gggtcggaga tcgcgggcat acccgcgtcg
      361 gagacaagcg cgcagctctc ccctccgagc agccggggga ggatgacggc ggcgctttcc
      421 accttgttgt gctcataata gctgatgagc gggcggcgga tgccgaagtg gttgaggagc
      481 tttaatgtga cgcgcgtatc ctccgcggcg atgaagtcca cgcttttgag cgtttcgatc
      541 gcgcgggggg agagatcgtt cagattgccg atgggtgttc ccaccagata caatgttccg
      601 gccataaaga ttatgcctcc cagtggcaga ttttccggta ttcttcggtt tcgcggccgt
      661 ctgcgccgcg cagcaggaca ttttcacagc gcatggcgct catcggcgcg cccttcacgg
      721 cttccatgag aaaaatgggc gcgagctttc ccggcgcgga gaaaacgtcc cgccggcgca
      781 ccggcgtgag atgggcgttg tgcagcgccg cgagaatgtc gctctcgcgc tttcgccggt
      841 ggacgagaaa aaattgcccg ccgggcttga gataccgcgc ggaggcggag cagagctccg
      901 aaacggtcgc gctttcggtg cgctgcgcgg cgcgctccgc gttgggcgat acggcgccgc
      961 gtcccgccgg aaagtacggc ggattggata cgataaaatc gagactttca ctctcaaacg
     1021 gcgcgctccg gtaatcggcg cagacggcct cgccgcgctc ttcaagcgca aaggcgcgca
     1081 gattctcccg cgcgcttgcc gcggcgctct cccggatatc cacgccggtc atgcggcggc
     1141 tttcatcgcc cagcaggaga agcaataaga gtatcccgct gccgcagccg agatctgcgc
     1201 cgtatgtccc gtgcgcgtct ttggcaaagt ccgcgagcag cagcgcgtcc gttccggctc
     1261 tctcgccggt aaaaagattc cagtccatgc aatcattata aaataaatac ggcggcatga
     1321 caagcccgaa agggcaggca taccgccgtt gtgagccgcg ggagtggcac ctcccgcagc
     1381 cgaagaagga ttactgctcc tcgatggcct cgatggggca gttgctggcg caggaaccgc
     1441 actgggcgca gacgtcctgg ttgatctcgt aatgatcagc gccttcggaa atcgcgtcgc
     1501 aggggcacac gctcgcgcag gtgccgcagg aaatgcagct gtcggtaatg acgtacattg
     1561 gtatgtacct cctctttttg ggatacccgc attgtaacat atatccctta aaatgcaacc
     1621 caaaaatttt tccctgccgg aaaaatttta aaaagcgaga aaatacgaga aaaacgaaga
     1681 aataatgtga atgagcgaga tggggaaaga caaaacgccg aatttccctc tttcggtgca
     1741 aagcgggcgt tttagagagt tgagaatttg acgtttcttg actattatac agtcaaagaa
     1801 aaggttaaag aaagtcaaag tcaaagataa cctgatcaag gaagggatgc cgctatgggg
     1861 acaagtgatc ggatcgcacg gttcattctg gacgcgctgg aaagcgcgga cggaacggcg
     1921 gagctgcagc gaagctctct ggccgaccgt ttcggctgtg tgccgagcca gatcaactat
     1981 gtgatcgcca cgcggttcag tcccgagcgc gggtacatgg tcgagtcgcg gcgcggcggc
     2041 ggcggataca tccgcatcac acgcgtgcag ccggacgcct cccagttcat catgcacacg
     2101 gtaaacgccg tgggccggga gctggataag ccgacggcgg acgcgctgct ccacaacgct
     2161 gtgaacgccg gtgcgctcga ggttcccttt gcccggctga tcgccgcggc gctcagcgag
     2221 cgggcgctcc acgccgttcc ggtcgagatc cggaagacgg tgcgctcatc catattcaaa
     2281 caaatgctgc tcaccacagc atccgacgga ttttttgaaa ggagatcatt acaatgaaat
     2341 gcgagatctg caagaagaac gaagctcagt tctattataa ggaatccatt aacggaaaga
     2401 ccacggaaaa gcacctgtgc gccgaatgcg cccataagga aggtctggac aggatcttcg
     2461 agcgcgaggc gaacagcatg ctgacctcct tcggcaagat gatggacagc ttcctgctgc
     2521 cggacgttga tttcttcggc tttggccgtc tgccgtcgat cggcgagatg ttcgagccgc
     2581 tgcggacgct ctcggagcat gagccggaag ccgaaatgca cgaggaggcc gacccgctcg
     2641 aggcaaaggc cgaggagaag ggcgaggaag ctttgggtca caagggcgag gagccgagcg
     2701 tgcgcgagga cgcctatccg ctggaggcgg aggcggagga aaacgccgag gaggcttttg
     2761 agcaacgccg cgagctgaac gctctgcgcc acaaaatgca caaggccgtc gaggaagagg
     2821 attttgaaac ggccatcgag ctgcgcgaca tgatccggaa aatggaaaac aaataaagcc
     2881 tttgcagctt tatatatcgg aacggagcgg atgcaaaatc cgctccgttt ttttaaaacc
     2941 acgcgctctg ccgctccgga tgattgagaa acggaaaatc tgtcaaccat cggcagggga
     3001 aggagacgac gaatatgaac gaccgattta ccgaaaaggc gagaaacgcc attgaaaagg
     3061 cgcgcgccgc cgccatggag ctgggccaca gctacatcgg caccgagcac cttctgctcg
     3121 gcatcgtgcg cgaaacggac gcgctcggct cgcgcgtgct gcgcgaaaac ggctttgagg
     3181 aagatcttgt gatggatctc atcgaaaagg ccgccggccg cggcgcgccg ggcatgccgg
     3241 tgcagggtct cacgccccgc tgcaggcgtg tcatcgagat cgccgtagcg gaggcaaacc
     3301 gtctgcgcca cagctatgtc ggcacggagc atctgctcat gggcatattg cgcgagccgg
     3361 agagcaccgc cgcgcacctt ttgactgccg ccggcggcga tctcaacaag atctatacgg
     3421 atgttttcac ccgcttcaca tcgagcgact accgcgccgc ggcgcgcagc ggcggcgtcg
     3481 ggcagcgcac ggcggtgcgc catgcggaaa cgaagaatct tgaccagtac agccgcgatc
     3541 tcacggagat ggcctcgcgc ggcgagctcg atcccgtgat cgggcgcgac aaggagatcg
     3601 cgcgcgtcat ccagatcctc tcgcgccggt cgaaaaacaa cccggtgctc atcggcgagc
     3661 cgggcgtcgg caagaccgcc gttgccgagg ggcttgccca gcgcgtcgcc catggcgagg
     3721 tgccggagaa tctgcgcgac aagcgcatcg tatcgctcga tctcaccggt atgctcgccg
     3781 ggacaaaata ccgcggcgac tttgaggacc ggctgaaaaa cgtcctcaag gaggtcggca
     3841 aggcgaagga cgtcgtgctc ttcatcgacg agctgcatac cgtgatcggc gcgggagcgg
     3901 cggagggcgc gatcgacgcg gcgaatatcc taaagcccgc gctctcccgc ggcgagatcc
     3961 agatcgtcgg cgcgacgacg ctctcggaat accgcaagca cattgaaaag gacgccgcct
     4021 ttgagcggcg cttccagcct gtcacggtcg ccgagccgac ggaggacgag agctttcaga
     4081 tcctgcgcgg cctgcgcgac cggtacgaag cgcaccacgg attgaagatc acggatgagg
     4141 cgctcaccgc cgcggtgaag ctctccgcgc ggtatatcag cgaccggttt ctcccggaca
     4201 aggcgatcga ccttgtggac gaggccgcat cgcgcgtgcg catggaaaat ctgaccgctc
     4261 cggacgagat gaagaccatc gaggcgaagc tcgcaacgct gtccgcccag aaggaggagg
     4321 ccgtccgcgc gcaggactat gagcttgctg cgcagctgcg cgaccgggaa cgctccgagc
     4381 gcggcgcgct cgaaaaggcg cgcggcgaat gggacgccga gcgcggcggt caccgcggcg
     4441 tcgtatccgc cgaggatatc gccgcggtcg tctccggctg gacgggcatc cccgtcacaa
     4501 gccttaccga gagcgagagc gagcggctgc tgcatatgga ggatgtgctg caccgccgcg
     4561 tgatcgggca gaacgaagcg gtcgcggcgg tggcgcgcgc catccggcgc ggcagagtcg
     4621 ggctcaagga cccgcaccgg cccgtcggga gctttctctt tctcggcccc acgggcgtcg
     4681 gcaagacgga gctttgccgc gcgctcgccg aggcgatgtt cggcgacgag aacgccatgg
     4741 tccgcgtcga tatgtccgag tatatggaaa aacacacggt atcaaagctc atcggctcgc
     4801 cgccgggcta cgtcggcttt gacgagggcg gtcagctcac cgaaaaggtg cgccgcaagc
     4861 cctacagcgt cgtgctcttc gacgagatcg aaaaggcgca cgaggacgtg ttcaacctgc
     4921 tgctgcagat catggacgac gggcggctca ccgattcgca gggacggacc gtcgatttcc
     4981 gcaataccgt gatcgtgatg acatcgaaca tcggcgcgcg agccatcacg gaccgccgca
     5041 cggcgctcgg cttttccggg ctggacggca cgccggagcg gagctatgct gagatccgcg
     5101 aggcggtgac cgccgagctc aaaaagacgt tccgtccgga atttttgaac cgcatcgacg
     5161 agatcatcgt tttccaccgg ctcaccggcg aggacatccg cgccatcgcc gggaacatgg
     5221 tgcagacggt ggcgctgcgc gctgccgcgc tcggcgtgca tctcacggta acgcccgagg
     5281 cggtcgcgag actcgccgag gccgggtacg atccggatta cggcgcgcgt cccctgcgcc
     5341 gcacggtgca gacgcagctg gaagacccgc tcgccgaagc gctgcttacg ggggagcttt
     5401 cctccggcgg cgaagcggag gcggtcgtgg acgagaccgg acacgtcgcg gtgcgcgcca
     5461 agggcacgct cacggcgtcg tgagcgatgt gccggggtag tagtgcgtta agatctcctg
     5521 ccacgtcgag ccgttttttg ccatgacgtt cgccccgtac tggctcatgc ccgcgccgtg
     5581 gccgctgccg gaaacggtaa agagaaagct gcggcccgtc cacgcgagac ggaagtttgc
     5641 cgagcgcagc gagaagatcg agcgcatttt cgtccccggt acggagacgt ccccgacacg
     5701 gacggacgaa acgcgcccgc tcggatcgac cgtccgttcg ccgatccagt ccggcggctg
     5761 ggcggagaga tcagcatccg gatatgccgc gagaaatttt tcccggaagc tctccgggga
     5821 aaactccgcg gtcgtgacga aattcggaac gtctgaggcc gtttcgggac tcgaaacgct
     5881 cacaagatac ggcacatcgc cgccccacac ggcggcgccg ctctcggtaa acccggcgct
     5941 ggaggcgtgg aagcacgcgg tgatcggctc gccgtcgtag acgagatatg ccccgtccgt
     6001 atcgcgcacg gcctcttcaa tgcgcgtttc ccagagatcg tactgcccgc cccagcgctc
     6061 gcggcgctct tcgaccgtga ggtacacctc acagcagccg ggatcgtcgc agagggcgca
     6121 ctgcgggtgc gccgccgggg gcgcgctccg gcgcgcgagc gcgtagcttc gcgcggcggc
     6181 ggcctgcgcg cgcagagcgt ccggctcaaa ggacgccggc atctccccgg cgacgacgcc
     6241 gggaaggtat tccgccatgg agagcgtttc cacctcgccg ccgcgcagca gggtgacgcc
     6301 cgtgagagca tcgaaaccgg aaaaagcgtc cgcggcactc tccggcgcgg cggacgcggc
     6361 ggggaagacc tccggcagcg cttcgccccc ggagatcccg gcggcgtgct gcaccgtgac
     6421 cggcagcagc agggaaaaga gcagcgacaa cacgctcatc agcatacagt gcttcatcct
     6481 atatacccct ctcttgacga taaaccaaaa ggagtataaa ataccattca tactccctcg
     6541 ttcccggcgc cggacaagcg gcgctcacgg gaaactctat gaggacaggg gctttcctat
     6601 gcagaaaaac gcatggacat atacgatggc ggggaccgtg ttcgccgcgg tcgccgttat
     6661 gctgcactgg cttcaggtgc agaagatctt cgatcccgaa accggccttc cgacgctcca
     6721 cgcgccgatc acgacgctgc tcgtcgtagt tctcgttctc gtcgccgcgg cgctctggtg
     6781 gctgtcgggc cgcatgaaga ccgactgcgc ccccgaagaa ccggaggacg cgctcgccct
     6841 gccgcaccgc gagaccggct ggatcctcat cgccgcggcg gcgctcgccg cgctcggctc
     6901 ggcgtacctc ttcttcaagg aggacagcac gcttatgaag gccgcggcgc tgctaggcat
     6961 cctgtccgcg cccgtgctcg ccatgatgcc gcagatcccc cgctggggcg gcttcggcgc
     7021 ggcgctcagc gtgctgccgg tgctgttttt ctgtctgtgg ctcgtggtgt tctataagga
     7081 aaacgccgcg aacccgatcc tctggtcgta cggtatggag atccttgcca ttgcgatgtg
     7141 ccttctcgcg gtgttccggc tgagcgcgta tcttttctac cgcgccaagc cgcgccgggc
     7201 gatcttcgca tgcatgctcg cgctcataac gagcctcgcc acgctcaccg acagcgtctc
     7261 tctcggcgcg cgcgccgtgc tcggcggctg gggtatcggc accgcggtca tgtgctggat
     7321 catcatcaac aacttcgttc ctcccgcgcc ggaggaagat acatattaaa aaagcccttg
     7381 tgtaaaggga aacccctccg tcaaaaactt cgtttttgcc acctcccctg ttaggggagg
     7441 caagtaagct atcggaaaac cggcttggct cccctaacag gggagctgtc agcnnnnnnn
     7501 nnnactgagg ggttcgcgca gaaaaagcgc ccgcaggaaa atgttcctgc gggcgctttt
     7561 gcgtcatttg ccttatttcg tatgcaccgt gagctgggga taggggatgt cgatgccctt
     7621 ttcatcgaag agcagcttga gagagcggtt gagcgcgcgg cgcgcggcga aaatgttctc
     7681 ctccgttgcg gagacaatga acttgagcac aacgccgctc ccggcgagct cctccacgcc
     7741 gagatactgc ggcacgtcga gaaatacgcc gcggttctct tcatacatgg cgggcagcgc
     7801 gtcggtgatc acggcctcca cggcgcgcag gtcggcgtca tagtcaatgg agatcgtgct
     7861 caccgccaca gatgtttcct gcgagcggtt ctggaaattc cggatctcgg aattgttgat
     7921 gatcttgtgg ttgccgccgg aatcctcgag cgtcgtggtg cgcacgctga tgtctacgac
     7981 cttgccgcgg aaatcgtcga gcacgaggat atcgccgacg ttgtagcgcc cttcggcgat
     8041 gaggaaaatg cccgtgagca catcctcaat gagcgactgc gcgccgaagc cgatgatgag
     8101 cgttacaaca ccgacgcttg cgagcgaggc cgttacgtcc acgccgagcg cgcgcagccc
     8161 gaagaagacc gagagcagca cggccgtata ggagagaatg ctccgcgtga gcgagcacac
     8221 cgtcctcgcg cgcgtctcct tcggctgctt taagagaagg gcgaacacgc cgtagccgat
     8281 ccagatgaag cccagatgcc ggaacatgtc ggccagcccc ggcgtgagcg ggcgcagaac
     8341 atagcccagc gcgcagagca caacggaaac gatcagcaga acgggaaatt ttttgacggg
     8401 tttcatgaag cggcctcctt atacggcgtt gtgcggacgg tctcggcaaa gtcaaagggc
     8461 tcggtctcgc cgtccggcag aaaatataca tggatggtca caagctccgc atgctcctcc
     8521 ggcagcggga gagtgacaat gcagctgtag tcgtttttct cgatggacgc ggcggaggga
     8581 tcaaagcctt caacctccgg ccagacaaaa acggtgtccg catcgagtgt ggatagatcg
     8641 tttccgacga tcgcgtcggg gatcgtgccg ccctcggcag gagtgagata aacgctcacc
     8701 gtgccgccat cggtgtcgta gccgcaggcg atcgtgaagc gctgcgcggc gggacgctcg
     8761 gcggcgagcg tttccacgcg cccgtcggcg tgcgtgatcg taacgcgcac cgacagcgtg
     8821 ctttccacgg cgctgtcggc atagaaatcc gggtgcgcct cacgcacgga ggaatacgta
     8881 tcgcgcatcg tatggtattt gccgctctca atgctgcttt ccggaatggg gtatgtgctc
     8941 acctcgtccg gaaagttggg atcaaagaat gtgatctccg cggaaacgat gtcctccggc
     9001 acggggaaac gcacctcgac ctcacaaagc gaggcgaacg agaaataccg cagcgtggga
     9061 accggcgcag aggcgggctc ttccgcgggc gtctcggtgg gggcgggcgc ggcggtcacg
     9121 accggcgcgg gttcagcgtc cggtgtcctg gcgggcgctt tcagcagcgc gagaaacggc
     9181 agcagcagcg ccgcggccgc gagcggcgca tacttcgtcc ggcggctgcg ccgaggctcg
     9241 tagggcggtt cgtattcgtg cgttgtctgg tatgtctccg gcgcggcggg ggagcgcagg
     9301 ggcttttccg agctgtattc ataaatatcg tcggcgatgg cacttcacct cccggaaaga
     9361 tatccatacg agtttatttt accattcttc cgcgccgcgc gcaacagaaa aggcgctgcc
     9421 gctttgttaa aacggcaacg gctttttccc ggtcacgccg gctgcggccg gatctctatc
     9481 gtccgtccgc cgaggttttc gtaaacgcgg tgcgaaacgg agagaacggt gcgcccctcg
     9541 gaggcgcggc gcaaggcttc gagcacgcgc gtctcggtct cggcgtcgag atttgcggtg
     9601 atctcgtcga gcagcagcac ggcgggatct gctgcggcgg cgcgggcgat ggagagaagc
     9661 tgccactcgc cctgcgagaa catgccgtcg gtgcacaccg tatcgtagcc ctccggcagc
     9721 gatgcgatgg ccgcgtcgat cccggcgagc cgggcggcgc tttttgccat ttcctccgtg
     9781 atgcgagcat cgcccagcgt gatctggtcg agcacggtgc ccggcacgcg ggagaaatgc
     9841 tgctccacgc agccgatgca cgcgcgccgc tcgctgtcgg taatatccga aacgtccacg
     9901 ccgccgatgg tgatgctgcc gctctccggc ggatagagcc cgagcagcag cttgaaaacg
     9961 gtgctctttc ccgcgcccgt ccggccgacg agcgtgatct gctcgccctg cttcacggtc
    10021 atggagaagt ccgtcaaaac gctcttttcc ccgtaaccga acgtcacatg cgagagcacg
    10081 atgtcgcccc gcacggcgct ctcccgctct tcggggatgg tacgctccgg ctgggcgaga
    10141 aaggcgtcga tacgctttac gcccgccatg gcggattgaa tggtctggat ctccatgccg
    10201 agactttcga tcggcgtgaa aatgcgggag atataatgga taacagtgac ggaggtgccg
    10261 accgacatgc cgaacagcgc caggatctcc gctttgccgg aggcggagag cagcatgacg
    10321 acgccgacca cggcggcatt caaaaggagc acgaccggcg aatagacggc gtcgtagaaa
    10381 tttgtccgct ctacggcggc atagctctcg ccgatgcgcc ggtcgtaccg gcgctccatg
    10441 tagtcctcca gagcgagcgc gcggatcgtt cggatgttgt gcagcgtttc cggcacctgc
    10501 ccggaaacgg cggcgacggc gcggcggtta tcgagctgcg ctgcgagcat ccgcttctgt
    10561 acatggcgcg tgaacacggc aaacagcggc agcaccagca gcaggatgag cgcgagaccg
    10621 gtgtttttca ccgcgatgac cgccataatg gagagaatgc ggcaggcgtc cgccgccatg
    10681 ctgatgatgc cggaggtaaa aagcgcctcc acggtgtcca catcgccgga aaagcgcgcg
    10741 gccatctcgc cggggttttg ccccgcgagc gtgccgacgg gcaaaagcgt cagcttccgg
    10801 gacatttccg agcgcagcgc gtgggtcatc ttctggccga acagcacgag caggctctcc
    10861 tgcgcgctgg agagaagacc ctccagcgcg agactgccga aatagagcag caccgcgagc
    10921 gcggagagcg gtatgccgcc ggtcagccgg tcaatgacgc gcccgagcag cagcggcggg
    10981 aggagcgacg cgccaacgct cgccgccacg cagagaagag tacctgcggt gagaagaggc
    11041 cgcgccgcgg cagcagaccg gatcgcggcg aacacgccgc tattctgctt tgtcttcatg
    11101 ctctgtccct cctgtctggc tttcgtagag ctggcggtac accggaacgg acgccatcag
    11161 cttcgcgtgt gtgccgacgg ccgtttttcc gtcctccata aaaatcacct gccgcatctg
    11221 cggaaaatgg tacagccggt gcgaaataag gaacacgacg ttatttttcg cgtagtcctg
    11281 caagttggcg aacaccgtgt cctcggtttt gcggtcaagc gcggagaacg gatcgtccag
    11341 aatgaggacg ggacgcgggt gcgcgagcgt tctcgccagc gcgagccgct gcgcctgccc
    11401 gccggaaaga cgtgtgccgc cgctgccgat catggtatcg agtccgtttt ccatggcgag
    11461 cacttcgtcc ttcagagcaa cggcggcgag atacggcatt gcgtcctgtt cgctgccgca
    11521 gaggacatta tggcgcaccg tatcggcgct gagctccggg tcgtggccga gatacccgac
    11581 ggtcgcggcg atctgccgcg gcgtgagcgc ggaaaactct tccggcccga attttgccga
    11641 gccgccgtag ggcgcttcgc agagaaacac gcgcccgaat gtggacttgc cgcaggcgac
    11701 gggaccggtg acgccgatga tgtcgccggg gcgcgccgtg atgctcaggc cggagaagac
    11761 cggctcatct ccgtaggcaa aggagagatc tttgagcgtc accgtttcgg gtgccgggat
    11821 atccagttcc gggagctcct ccggcttttt catcagcggc ttgatacgct tccaggatac
    11881 ctccgccttc tgcacggagt tgaagagctt tgcggccttg gaggatttga ccgtcagctt
    11941 cgtaaagcag gagaggaacg tagtgaacgc cgcaatgtcc caggtgttcc atccggtacc
    12001 gagaacgttt tttgcgccca gccacagtat gaacagcacg cccgcgttgg acacggccag
    12061 atacagcggc ggcagcgccg actgctgcac gctgctgcgc acggcggcgg tctcgtaatt
    12121 tttcagcgtg tcctcatacc gctccgcgcg ggcgtcctcg cagccgtaga tgcggtaggt
    12181 cacggcgttc tgcgcgcggt cgagcgtggc ggcgctgagc gcgcccgcgg cctttttata
    12241 cgccgcgccc gcgcgctgca ccggcttttt catccgcgcg gcgcatacat aggataccgg
    12301 cgtaaaaagc aaactgagca gcgcgagccg ccagtcgtac acgagcagca tcaccatata
    12361 gccgacaagc gccacgccgg tatcgaagat ctcggtggtg aatttccgca tgccctccac
    12421 gcagtcgtcc acgtcggaga tggctttggt catcagctcg cccgcgccct ccttttcgag
    12481 agacgcgcgg ctctgccgca ccagaccagc gtaaaggatg cccttcatcc ggcggttgat
    12541 gttgttggca aagcggcgga cataaaagcg cttgacaaag cgcgcggcct gcacgatgag
    12601 cgtaacaaat atgtaggcca gcaccagaac cgccatcttt gcggcggtct cgctgccgcc
    12661 aagaatgtcg gcaaggcact gggcaagccg cccctcaaac cacggcgtag caagaaggcc
    12721 gatgttgtaa gcaaggcccg aaagcgtcac aaaggccagc ggcagccact ctgcgcggaa
    12781 ataggacccg attttgtccg ggcagcacgt tttttcacgc ttcatcgtct gcttcactcc
    12841 ccgatgcctt ctgaaaatgc gggaagcccg cgcggctttc cagcttggag gctgtgtaca
    12901 gcttctggag cgccgcggca aaggcggcag cctcctcctt ggaaagcgta cttgtcagat
    12961 attcataaaa ggccgcttca atctccgcct tggaggattt caggctttcc gccttttccg
    13021 tgggatagag cagctggctg cgccggtcat tcggcgcgtc tttgcggaca agatagccct
    13081 ttgtctccaa gtttttcgtt ctgcgcgcaa tggccgcctt atcggcatgc agcagctctg
    13141 ccagcgccgc ctgcgtgcag ccgggatggt ggcgcagagc gtgaatcagg tcaatttccg
    13201 ccgtgccgac gccctcttct cgcagcgtgc gcagaaccag catttcagcc tcgcgcgcaa
    13261 tttttgtgat tttccgttcc gtaatatcca tagctgcctc ctgattggtt tcaagaagat
    13321 gtatctacat cgttgtatgt acaacatatt acggcacgct ttttgcattt gtcaatagcc
    13381 gctggaaaat cgatctgcaa aaccggcaac tgatcactgt ccgcttgccg caggcaacat
    13441 attttctgcc gcttctgcca acaatatatc ttgtcgaatg cttcataaag ggttataaca
    13501 agtgccatac aaaccttggg aggtacagcc atgaaacttg cattttttga tacgaaaccc
    13561 tacgacaagc ccggctttga cgag
//
LOCUS       DAWAAE010000042        13508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2037_length_13508_cov_3.304988, whole genome shotgun sequence.
ACCESSION   DAWAAE010000042 DAWAAE010000000
VERSION     DAWAAE010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_13508_cov_3.304988"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    493..502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4889..4898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2626 a         4196 c         3910 g         2756 t
ORIGIN      
        1 gtacgacatg tcgtcccggc ggctcggtct ttttttaagc agccagatct tccgtgtaat
       61 acgtaatgga ggaaatgccg gtgtagaccg tgttgtttgc gatcttcggc tcggcaatct
      121 cccactggtt gcccttcacg ttcgtgtgga gggagtacat accctccgcc gaggaggaga
      181 tgtccacgcg gatgtaatca cccggattga gctagcagac gacggagccc gtctcgaagt
      241 gcagtgtcac cttctcgccg ggggtatagt caccgagctg atagctcagc gacgtgatgt
      301 cctcgcggag gttatacgct acgccgtttt tcacaacgtc gatgcgcacg atgaagctcg
      361 tatcctcgca atcggaggaa accgccacat ctgcagtgag aatgcccttg atgaaggtct
      421 tttctcccac cggcgcggag acgaagctga ggatatcctg ccgggaattc ggctcggcgt
      481 cagtgccgag acnnnnnnnn nnagaccgcg gtagccgttc gagccgttgc cgccgttctc
      541 atcgctcgcg gtaaagagtg ccggattggt cgggtcatag acatacgtca gcgctccttc
      601 ggcttcgccg tcggtgccga gcgtgctgcc gccgttgaga tagagtacgt tctcggtcgt
      661 gccgttcgtg atcgtcgtat cctcgctgta ccaccgctca ccgtccacgg ggtaatacgt
      721 caccttgcca agttcgactt tcgtcggctc cgtcccctcg cgcacgctgt caaagaaatt
      781 gacgacataa tcggcgttgt aggtcggcgt cagtgaatcg ccgatattca gcggccaatc
      841 cgcatagccg gtataggtgt gaccgaactg gctcacgaca aaggcagact gcgccttctg
      901 ctcatcggag aagccgttcc agaaattcat gctgtcttca tagaaaatat cgtagaagtt
      961 ttcaaagagc agcaccggca catcgatcga agcgattgcg tcatagatat agcttcccgc
     1021 cgcttcggtg cgccaccagt cgccgtcctc cggatgcgtc aggatgcccg tccagtaatc
     1081 ctcctgctcg ccgtacagct cgaccggcca gttgatatag ggaaacgtat tgaaaagctc
     1141 cataccgccg accttctccc atgcctcggc cggattcact ccaacggggc ggtggaagcg
     1201 tccctgccag aagcccatga gaccgagctt cacaaagccg ttctggtacc atgcatcgta
     1261 gcgtgtgctc acggggcact gcatggagat cgccttgata tcgtcatggg ctgcatacag
     1321 gctcgccata gaggtgaagc ccatatagct caggccgttg cagaagatct ccccatcgta
     1381 aaagtcctgt ccgcggatcc agtcaagcac ctcgagctta tcgtccgtat cgtggatata
     1441 cggaaggaag ctctcatacg ggctgttgcc cgttccccgc gtctgcatca taacaaacgt
     1501 gtaccccgcc tcgacaaacg cggagctgtc cgacacgcac ttctcgatgt actcggcgtt
     1561 gaactcctcc gttgccgcct gatagcagtt ctgggtaatg accgtcggga actgtccctc
     1621 tttttccggc ttgacgatca gcaggcagat ctctgcgccg tcatccatcg gaacaaattc
     1681 cgtgtaagca tacccgccgg agcattcgat gtactctctt tccaccgtct cttccgccgg
     1741 agctgccgtg accgccgctg tcggctctgc cgtggcagcg ggttcgctta cggtgccgca
     1801 ggcgcacagc gagagcagca tcgccgcaca cagcagcgcg ctcaaccatt ttttcattga
     1861 cctcgtccat cctctctttt cattttcttg acacttttcc cagaaagtgg tttaatattt
     1921 tcactttctt tgtgtcatct gtacccatcg tagcgcagaa aacgggctaa aacaacccgc
     1981 aatacctctc cgttttctcg aacaggtcga ccacatttat tcggacattt tccccgattc
     2041 cgtcggaaaa ggagccacgc atgaaaaccg gagcaaaagg aaaaatatgc caagcccttc
     2101 tggagctggc tgacggaaag tccgcacagg atgtcaccgt ttcggagctt attgcgagag
     2161 ccggtatcaa ccgctctacg ttttattatc attttaccgg cacacacgag gcgctggaat
     2221 acatgatgga tgatttctgc gcgcaatacc tttccgcgct gatgataccg cgcggtgagg
     2281 ttgccaagag catttcgaaa agcagtcagg atgcgctgga aaagggtgtt tgcgattata
     2341 tccgcaaggc cgggcatgtt gttccctttt ttcttcagga accgcactac cggctgtttg
     2401 cgaaacactt cagaaaagcc tttcaggact actgccaggc gcacacgatc gttcaggtgt
     2461 tccccgacgg acacactgaa cagctcaagc gcggagtgtt ttacgactat tacgtacaca
     2521 tgagctgttt gcggcttttc gccgtgctcg aatgctgggc cgagcggaat ttcagtgaga
     2581 cggaagagga ttttgtccac atttttgaca cacttcacaa cacgtcgata tcgtttcagg
     2641 gctgaagaac tatcgccggt ctcccgcgtg ggagaccggc gatggtattc tgttatgatc
     2701 acacaatgcc ctgatgcagc atcgcgtccg cgatcttgag gaagcttgcg atgtttgcgc
     2761 cgacgacaag atttccgggc tgtccggcct cgacggaggc gtcgtaagcg gccttataga
     2821 tgccgcgcat gatgttgtgc aggcgctcgt ccacttcctc gaacgtccag ctcaggcgca
     2881 tgctgttctg ggtcatttcg agcgcggagg tcgaaacgcc gccggcgttc gccgccttgg
     2941 ctgggccgac ggggatgccc gccgcctgca gcatggcaac ggcctcgagc gtgcagggca
     3001 tgttcgcgcc ctcggtgcgg atcatggcgc cgccgtcgag gatcttcttc gcctcagcca
     3061 cgtccacctc gttctgcgtc gcgcacggga gcgccacgtc gcacggcaca tcccagatat
     3121 tgcggcagcc ctcgtgatac gtcgcgcccg gcatctcgtc ggcatagacc ttgatgcgcg
     3181 cgcgacggtt ctgcttgatg tccttgatga gatcgagctt gatgccgtcg ggatcgtgga
     3241 catagccgtt ggagtcggac atgcacacga ccttaccgcc gagctgcatg gccttttccg
     3301 cggcgtaggt cgccacgttg ccgctgccgg agacgatgac ggtcttgccc tcaaaggagg
     3361 tgttgagctg gtggtggagc acctcgtcgg tgaagtagca gatgccgtag ccggtggcct
     3421 cggtgcgcac gagcgagccg ccgtaggaga gatgcttgcc ggtgagtgtc gcagcgtcga
     3481 actggttggt gatgcgcttc atctggccga acatatagcc gatctcgcgg ctgccgacgc
     3541 cgatatcccc ggcggggccg tcggtaaact ggccgatgtg acggtaaagc tcggtgatga
     3601 acgcctggca gaagcgcatg atctcgttct cgctcttgcc gtgggggtcg aagtccgagc
     3661 cgcccttgcc gccgccgatg ggcagcgtcg taaggccgtt cttgagattc atctcaaagc
     3721 cgaggaactt catgaccgag aggttgaccg acggatggaa gcgcagaccg cccttgtagg
     3781 ggccgatggc ggagttgtac tgcacgcggt agccgcggtt gacctgcacc ttcccggcat
     3841 cgtccaccca tgtgacgcgg aacgtgatga tccggtcggg ctcgacgaag cgctcaagaa
     3901 tggcgttccg ctcccactcg gggtgcttat ccacgatgcc ctcaaggctc tcgagaaatt
     3961 cccggatgca ctggtggaac tccggggcgt tggggtcgcg cgcgagcacc tgatcgtata
     4021 cccgctggag gtaggcgttt ttgagcatgg atatgttctt cctttcggtt tttattactt
     4081 gcggcagaat gcgacgagct gctcggcgga ggtaacaccg ttcgcctcat attcctcgtt
     4141 gagccacgcg gcctcggccg cgaggaaggc atgcgccggg ttgctctcgt acttcttgag
     4201 attttccggc acggcggcga tgcgctcgcg cagcttggcg acgaccttgg gatcgtcgat
     4261 gagatcgcag agcgtcttct ccacggtcag ccccgcgttg cgcgcgagcc agaggatctc
     4321 gctcttcgag tacgtcgggt gatggtaaat gccgtcgatg gtgtgcatcg tgcagtcgat
     4381 atcgtggagg atccagtgcg tgaggctcgc cttgggctgg ccgtcgttgt acatctcgct
     4441 gaggataatg agtccgccgg gcttcacgac gcgcttcatc tccgcgagca gcgccgcgag
     4501 atcagggatg tggtgcaggg agttgccgat gccgacgacg tcgaaggtat tatcctcgaa
     4561 atccatatgg cacgcatcgc cgagaacaaa gttcaccggc ataccggcgc acttctctct
     4621 tcccttttca aacatcttgt cggagcggtc gagcgcaacg atctccttgc agctgccgaa
     4681 cccggcgtac atttccttgg cgaacgcgcc gtcgcgcgtg gcgatgtcga gcgccttctc
     4741 aatatggagc gaagaaagct cctgccgcag tttatccatg agggatccct cctgttactt
     4801 ttacttgata tttatttaaa tgcgaatgct ccggttcccg caaagggtcc ggtacatttc
     4861 attataccga tgcttctcat tttttgcann nnnnnnnnca agtacgaact taggaaattg
     4921 cgtttttcag ccgtccgcag cccccgtcct gcgtcctgcg ccatatgtgt gcattatttc
     4981 ccgcaatttt ataaatacat ccgattttct tatttatttt caaaaagagc ttgctttttt
     5041 cggatgggta tgctatatta tttttcacag agaataattc tcctttaaag ataacttcat
     5101 tgctgctttt ctccttctct tcagacagac cggagcaggc cggaatcccc cttccgcccc
     5161 gctccggtct gctttttgct tttttccgca taagatccgc aaataaaacg ttaaaaatac
     5221 gttttcctgg aacggtttcc cgcatttgtc atcttgacgc ggcgggatgg catccgatac
     5281 aatttaatgg aactcattat attgtaaaag gagaacgacg acaatgtgga tcacgacagc
     5341 cgcgcagctt gcggaaaagc tgcccatgac agccgaagag cttgccgccg tacaggcggt
     5401 ggcagaccgc taccccatgt gcatcacgcc ctattatctg gggctgatcg acccgaacga
     5461 cccggacgat ccgatccgga aaatggcggt gcccgcgccg attgaggagg aggaaggcgg
     5521 ctcgtttgat acgagcggcg agcagtccaa cacgcgcgtc cccggcctcc agcacaaata
     5581 cctgcaaacc gccatggtac tcacaacgaa ccgctgcgcc atgtactgcc gccactgctt
     5641 ccgaaagcgc ctcgtggggc tgacggacga ggagatcgcg gcgaactttg agcgcgtgac
     5701 cggatacatc cgcgagcatg aggagatcac gaacgtgctc ctctccggcg gcgattcgtt
     5761 cctcaacagc aacgagacgg tcgcgcgcta tctcgacgcg ctcacgccca tcggccatct
     5821 gaactacatc cgcttcggca cacgcacgcc cgtcacctgg ccggaacgga tcgacgaggc
     5881 gctctgcgaa acactcgcgc gctatacggc gcggaaaacg atctttgttg tgacgcagtt
     5941 caaccacccg cgggagatca cgccggaatc cgccgccgcg gtcgccgcgc tgcgccgtgc
     6001 cgggtgcatc gtgcgcaacc agaccgtgct gctgcgcggc gtgaacgacg atcccgcggt
     6061 gctcggtgag ctgcttgcct cgctcaccgc catcggatgc ctgccgtact acattttcca
     6121 gtgccgtccc gtgacaggcg tgaaaaacat gttccaggtg ccgatggagc ggggcatcga
     6181 gatcgtcgag aacgccaagc ggctcgtaag cggcatggac aaggccgtta aatactgcct
     6241 gtcccacccg gcgggcaagc tggagatcct cacgaaaacc ggcgagaacg agctgctttt
     6301 ccggttccac gaagcgaagg atcccgcccg ctgcgggcag gtgttccgtc acaggacgct
     6361 cccggacggc tgctggctgc cggacgagct gacgctcgac tgaaaatatg tcagaaacga
     6421 gaaacgccgg gacgatgtcc cggcatttct tatggttcgg cagttttttc catctcgtgc
     6481 tctattctcg ccatcagctc gctcggtcgg atgcgcagcg cttcagcgat cttaaaaaag
     6541 gtatccagcg tcggacgtcg ctcgccccgt tcaatggcac tcaggtgcgt ccggccgatt
     6601 cccgcaaagc cgctgacaac ttcctgtgaa agtccctttt cttcccgctc ctgctgtatc
     6661 acgtttccca caactaccgc atccagcatc cttacacctc ccgttcagat agagtgtaag
     6721 gatttctttc gtcttttatg aatacatatg ttgtcaatta aacgcaaatg tgttagtatt
     6781 gactcacact accaaaaaca agcaggaggg aatttcatga gaaaaagatt ttggctcttc
     6841 gcattgcttc tcgcgctgtg tctggtgctc accggctgca ggaaaacggt atccgccccg
     6901 ccggctgctc ccggcagcac tttcgccgcg gccccggcaa acgacgcccc ggcgaccgtc
     6961 gcggattttg atgcggcgct gaaagccgca ggcttcatag acgaggacga atacgatgct
     7021 ctctatctcg accgcttttc cgacgacgca gcgctgtact aacaactcac gcaggatgga
     7081 atcgtcggtc tgctcgcccc cggcagttta gatgccgaag aaatgaacag caacagcgta
     7141 aagaaatacg tctcctacca tcttgaggaa atgtacatgg acgacggcaa gctgatgttt
     7201 ccgtggggcg atgcagtctc gtttcttgta tttgactcgg atgcctctgc cgagaatatt
     7261 tttcaatctg ttgtatccgc aatgagcgcc gacgatatgc cccctgaagt ccgcacggtc
     7321 ggaagcgcga ctagggctga atacagcggc gacaggtact cggttctctt ttccttgaaa
     7381 agtaatacga ttctggcata ccaatatcac agtgacagca gcgtaatggc cggcgtgatc
     7441 cgcgacctcg gttattgatc ggagggcagc aaatatgaaa cgacgctttt cttttccggc
     7501 actcgtcctg ctcgcccttc tctcggtact gcttctgccc ggctgcggca gctcctctcg
     7561 tcaggtcccc gattccgtga ttgaacgcga caccgtcgac tgcgattacg atgccggata
     7621 ctcccgttca tggacggcgg tgcatgatta tgacagttca tcccaaaccg acaacgtcgc
     7681 catcacgctg acgctctcct ccgattgcgg aacgatgacc tgccgccgca cggtggtcta
     7741 cgcatacgac aaaagttccg gcctatggtc tgccgtccgg cgggaaagct ggtcggatcc
     7801 ggaatacgct ttcggcaaag gtctctgcgg aacatatccg ctcaccaacg gagaagaggt
     7861 gtatgaagtg aagatcacct cggtcagcgg gaactccatc cagctcgaat gctgggcgga
     7921 cgccgtgtgt cagcggggat acttttccgc cggcacctgc tatctggaag catccggcgc
     7981 atattccgcc aacgggcggt atctggaaat cccgctggaa ctgccagacg gttactacgc
     8041 aaaataccgc gtcggcgata cgaacacatc ggtcactgac cgcgtcacgc tgaacctgta
     8101 tctggacatt gccgacggta ttttcactgc ctttctctat ccgaccgtcg gcgtttcgtg
     8161 acccgtcgcc acgccgcaac acgctcaagt cccccgccgg aggccatctc cggcggggga
     8221 cttttcacac tccatccgtc ttttctgcat tttcgagctg cggcacgatg acgccgcgcg
     8281 gctctgccgc cgtgagcgtg aacgtatcgc ttttcagaaa gtgtactttc tccgcggcaa
     8341 aaagcgcggt gaatgcgtcg agcgtgccga gctcgtcaaa gccgaagccc tcgccgcggt
     8401 agtgcatcgg cacgatgcag cgcggcgcga tcttcttcgc gatggcaaac gcttcttccg
     8461 cgtccaccgt atagtatccg ccgatgggca agagcagcgc atcgcacccg tgcagcgcgg
     8521 caatggcgct ctcctccggc acgcagccgg tgtctcccat gtggacaacg cgcagcgagc
     8581 cgaagcaaaa ctgccggatc gtgttcatcc cccggcgcac gccgccgtgg tggtcgtgcg
     8641 gcacggcaaa ttcctgcacg gaaaaatcgg gcggcgcttt ctcccgggtc tgcttcacgc
     8701 acgccgcggc gctgtggtcg ccgtggccgt gcgagcagta tacggcgccg gcgctcagac
     8761 aaagcggcgc aaggccgggg acatacccgt cctcataggg gtcggtcacg atggagtatt
     8821 cccgatactc catgcaaaag cacgagtggc cgagccattg gatcttcact tcattcatgc
     8881 gtctgcatct cctttcgcgc cgcggcgagc agcgctccct tttcgttcgg cacctcttcg
     8941 tccagcacaa gatcgagcag ccgggagagc gtctccccca ccgccgcccc cggcttcagg
     9001 ccgagcgcca gaagatcgtt cccgctcacg gcgaggtcct tgacgtgcag gcagccccgc
     9061 tcggcggtga tctcatgtaa gatccgctcg tcctcgtccg caagtgcggt cagccgcgcg
     9121 gcggcttcgg gcgcgtgcgc cgcggcgtcc gcgcgccgga cgagcagcag atcgcgcata
     9181 ttcttttccc cgatcttcgc cagcaggcgg cgcagatcct ttttgccgtg gccggtgcgc
     9241 atctcgtggt tttccacaag cgtgagcacc cgctctcgcg cggcattatc gcagcgaagt
     9301 cgccgcagcg ccgcgtccgc aatgcgcgcg cttgccgccg gatggccggg gaaacggccg
     9361 aatccggcct gcgcgtcata ccggaacgtc tccggcttgc cgctgtcgtg aaagagcatc
     9421 gtaagccgga ggacgctctc gggcgcgatg gcgtccacgg cgtgcgccgt gtggccccac
     9481 acgtcccagc agtggttctg cgggcggcgc gtatcaaagc cccgcatcgg cgcaagctcc
     9541 ggaaggatca caaaaaacac ctccgggaag gcgagcagca tctcccccgc gccgcgcgcg
     9601 cagaggatgc ctttgagctc cgagaaaata cgctcggggg aaatattgcg cagcagctcc
     9661 cggttttccc gcagcgccgc ggcggttttc tcctcgatgg tcagaccgaa ccgcgccgcg
     9721 aagcgcagcg cgcgcaaaat gcgcagcgcg tcctcccgga agcgctcgcc ggggtcgccg
     9781 acgcagcgga ggacaccgtt cctgagatcc tcccgcccgc tgaaatcgtc ccgcagaccg
     9841 cgcacggggc tgtaggccat ggcgttcacc gtaaaatcgc gccgcgcgag atcggcgtgg
     9901 atgtcgcgca cgaacgaaac gcggtccggg tggcggcagt cggagtagcc gctctcggtg
     9961 cggaacgtcg tgatctccag agggccggac ggtgcgagca acgtgagcgt gccgtgcttc
    10021 agaccggtct cgatcacgcg ctctccggca aagacctttt tcattgcctc cggcggcgcg
    10081 gcggtgcaga gatcccagtc gtgcggctcg atgccgagca gtgaatcgcg cacgcatccg
    10141 cccacggcgt agctctccca cccggcgctc tccagttttt ccagcgcgtg agcggcgtcc
    10201 tccggcagtc tcatcgcggt ccctcccttt cctttttctg cagtatacca cacgccgcgc
    10261 cccgccgaaa gcccccggcg ttgacgcgcg gaaaaaaatc gggtatattg ctcatatatt
    10321 ctttctatat gagaggctct gcctatgaaa aagcaaggca tccgcactct tgccacggcg
    10381 ctgctcgcgg cgctccttct cggcgcgctc ccggcgctcg ccgccgacgg ggagggcggt
    10441 gcggaagaaa gcagcacgat cgactttctc tcctacgagg aagcggtgac gtatttcgac
    10501 tttgacaccg ccgcaagcaa ctggtgcacg aatttcggca tggatcccac cgtgtcgctc
    10561 gaggaggcaa aggccgccat tctccacggc acggaccgcg agctcgtcat ccggctcgcc
    10621 acgttcgcgc gcgacgttgg ttacggcgac gaaaagctca tcgtgacgaa tgccttccgc
    10681 ccggcgtgct atcaggaggt catcggcctg cacgactcca acgccaacac cggccccttc
    10741 cgccgcgcgc tgctctggaa cggacggagc gtcacgaatt tctggtggga cgccgagacc
    10801 gcccccggct ggccggaaga ctatgccatt gacctttccg cctacgatat ccagacgctc
    10861 gatctgcgct actactaccg cgccgcgctg cggctgtggg ataacacctg ggcgaacggc
    10921 tactacgccc gtcccggatg cagcggccac aactccggca acgcgatgga tatatcgaac
    10981 tactggctcg gggcgaattt caaaacgagc tacgccaacc ccaagaccgg cgtcgtgtac
    11041 cacatggcgg actacgggct ttataagccg ctccagccct cggcgacgac cgcgggtgaa
    11101 acgtggcaca tcacgtcctc gcaggaggtg ctcgcgctcg gcaattacga caacgccctt
    11161 ctctccggct acgagatcgt gtacgcgctc tattataacc cgtcgctgcg cggctggtcg
    11221 atggcggacg ggcgcggagt gtacatcggc gcaggcgtca cggtattgca gatcgagctc
    11281 tgccgccgcg ggctgctcga ggaaaaatac atcaccggct acttctgctc caaaaccgac
    11341 gaggccgtgc gcgccttcca gtcgcagcag ggactcgtgt cggacggcat cgcgggcagc
    11401 ggcacggtca atgccttgct cagcgaagag ccgcgcaccg gcgacacggc cgcgcccgag
    11461 ctgacggcct cgaccatcgg cgcggcgacc tccaaaggct tctcgctctt cctctccggc
    11521 acggacgaga aggggctcaa cgccttccgc gtcgatacac gcggcgaggg cgacgacgtg
    11581 tgggtcacgc gctactacaa cgcccccgcc tccggctccg gcacgctcga cgtggacatc
    11641 tggcggcagg gcaattatgc ggtgcgcgcc gccgcgtgcg acacggacgg aaacgaaagc
    11701 ccgcttgccg acgtcggcac ggtgttcatc gatacgacaa gccccgtcat ccgctcgctc
    11761 acgatcaccg acatcactga gaccggcttc tcgctgcacc accgcgtcac cgataacggc
    11821 acgctcgcgg ggctgagcgt cacgctcacg tcggatacag gggaaatgcg cagctccttc
    11881 ctcgtcaccg acggcgagag cgatttcccg tggtcggcgg aggggctgac cgagggcgtg
    11941 tggacgatct ccgtcacggc ctccgacgcc gccgggaacg agagccgcta cacgttcgac
    12001 tggaaataca ccgccgggac cgcgcgcccc ggtctcaccg tcgcccgcct cggcgcgccg
    12061 taacacaaag gtgcaaaaag ccaaaaatgt ataaaacgac ctcctctgcg gaaacactaa
    12121 cgtttgccag aggaggtttt gctgtatatg cagggaaagg tagagatttg cggcgtcaac
    12181 acctcgaagc tcgccgtgct cgacagcgcc accacgcgcc ggctgctgct gcgcgtccgg
    12241 cagggcgaca agtccgcccg cgaagagctg atcgcgggca atctgcggct cgtgctgtcc
    12301 gtcatccagc gcttttcctc ccgcggggag aacatcgacg atctctttca ggtcgggtgc
    12361 atcggtctca tcaaggccat cgacaacttc gacgccgagg cgcacgacgt gcgcttttcc
    12421 acctacggcg tgccgatgat cgccggggag atccgccggt atctgcgcga caacagcgcc
    12481 ctgcgcgtat cgcgcagcgt gcgcgatacc gcgtacaaaa ttctgcaggc gcgcgaaaag
    12541 ctcaccgccg agaccggccg cgagccgacc gaggaggaga tcgcagcctc tctcggcatt
    12601 ccccggcagg agatcgctct cgcgctcgac gcgatcagcg accccgtgag tctctccgac
    12661 ccggtgtttt ccgacggcag cgagagtctc acggtcatgg atcaggtgcg cgactcgcgc
    12721 aacacggacg agagctggat cgagtgcatg gcgctcgacg acgccatgcg aagtctgacc
    12781 gggcgggaaa agcgcatcct ctcgctgcgc ttttacgacg gcaaaacgca gatggaggtc
    12841 gcctcgatgg tcggcatatc gcaggcgcag gtctcccggc tggagaagaa cgccatccgg
    12901 cagatcaaaa aggccatttt cgtatcatag gcgcggcggt gccctggcat aggacaacgc
    12961 cccgccccca taggctctat gggggtggta catcatggag gtacggctta ccgagctgcg
    13021 ctaccgcgaa gtcatcaaca tttccgacgg cacgcgtctc ggctttgtga gcgacgcgat
    13081 cctcgatctc acatcggggc gggtgctggc gctcatcgtg ccgggcccgg cgcggttttt
    13141 cgggctgttc ggccgcgaga gcgattatat tctcccgtgg gaatccattg cccgcatcgg
    13201 tggggacatc gttctcatcg agggcgccgc ggagatccgg cgcgaccggc agccccggcg
    13261 aggtatctgg ggataaaaac aaaatatttt ttcaaaagaa cgcaaaaagg cttgcagagt
    13321 gcgcgggcat ttggtataat acccgcgcat tacgcacggg gagcggaccg gcgaccatgt
    13381 ggcgaaagcc tggtcgcgcc gggttgaggc ccccggaggt aataacaaaa taaaggagga
    13441 acccccaaca tggcagtagt atccatgaag cagctgctgg aagccggtgt ccacttcggt
    13501 caccagac
//
LOCUS       DAWAAE010000043        13474 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2052_length_13474_cov_2.981966, whole genome shotgun sequence.
ACCESSION   DAWAAE010000043 DAWAAE010000000
VERSION     DAWAAE010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_13474_cov_2.981966"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1286..1295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6158..6167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9985..9994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2735 a         4131 c         3815 g         2763 t
ORIGIN      
        1 acgcgccgcg gcgctctcct cggcgcggca ctccgcggca aagcggcgcg ccgtttccgg
       61 cgcgagcggc agggcgtttt ccttcgcgct ctcatagagc cgcacccgcg cgcgagccaa
      121 aagcacgatc tgccgcagcg cagatagaac gcccggcgcg gtgccggagc gaagctgcgg
      181 cgccctctcc ccttcgcgga ggaaaaactc ggcgtgcagc gcccgccgcc gcgtctcaca
      241 gcggcgcagc gcttcgccga aagcattctg acatgcgcgg ctcgtgcgca taagatagcg
      301 cagccgtgcc gcggcctctg cctccaaccg aatggccttg cgcagcgcat ccggtggttt
      361 tttctcctct gtcggcgcag cgggcccata tacgcgctcc aatacgcgct tcatgcgctc
      421 ggtatccata cgaccgcctc cccggtacag tctattccgc cgccgcgctt cggttcccgt
      481 cttgcaatcc gcgccaaact atgccataat aagaaataat gttatgtgtg ggaggatgtg
      541 ctcatgccga gattctttct ctccggcgga agctcgatcg ccgggggcac ggcgtttatt
      601 ctgggagagg acgccgcgca cgccaaggtg ctgcgccttc gcgtcggcga cacgatgatc
      661 gtgtgcgacg gcggcaagga ccaccactgc accgttacga agatcacgcc caacgaggtc
      721 gaggccgaga tctttgaggt cgtcccctgt cagggcgagc cgagcgtgca caccgtcatt
      781 ctcgcgggcc ttcccaaggg cagcgacaag accgactaca ttcttcagaa atgcaccgag
      841 gcaggcgcat cggagatcta tttctttgcc tccagccggt gcgtggcgcg catccagggc
      901 gacgcggaca aaaagatcgc ccgctggcag cgcatcgccg aggaggccgc caagcagtcc
      961 ggccgcggcg tgatccccgc cgtcggattt ctgcccgatc tcgcggaggc cttcaacaag
     1021 gcgaacgcca ctgcgctgcc gctctttatg tacgaaaccg gcgagcgcga aacgctgcgc
     1081 gaggccgtgg aaagtgccgg aaatatcgcc tccgccgcca ttctcacggg tccggagggc
     1141 ggctttgaac cgtttgaggc ggagcttgcc cgcattatga agctgcacat ctgctcgatg
     1201 ggcccgcgca tcttccgctg cgagaccgcg ccggtcgccg cgctctcggc gcttatgtac
     1261 gccaccggca atatgtaaaa aactcnnnnn nnnnngttct gttacaatac gttccgtcaa
     1321 gaacatttgt tgttaactca aggacatttg accaatcaga gaaagaaggc gatgccgtga
     1381 aagctgcggt tttgggatac ggtacggtcg gcgcgggcgt ttattcgatg ctgtcgtacg
     1441 cccccggcat ggaggcaggg ccggtgctcg tgcgccccgg aaaggcggtc gagagcttca
     1501 tggtgagcga cgccgcgctc attttcggcg atccgacggt cgacgccgta gtggaaacgc
     1561 tcggcgggct cgacccggca tattcctatg gtatgcgcgc gctcgaggcc gggaagcact
     1621 tcatcaccgc aaacaaggtg atggttgcat cgcacggtct ggagcttgcg gcgctcgcgc
     1681 gggaaaaggg cgtggcgttt ctcttcagcg cggcgtgcgg cggcggcgtg ccgtttctcc
     1741 acaatctcgc gctctcggcg gagaccgacc gcattgaggc cgtttccggc attttgaacg
     1801 gcacaacgaa ctacatgctc gacgccatgc agcgccgcgg cctttcctac ggcgacgcgc
     1861 tccgggaagc gcagtctctc ggctatgccg agtctgaccc gacggcggac gtctccgggc
     1921 tggacgcgct gcggaaaatg cagctcgcgt gcgcggtcgc cttcggggtg ctgccgcaaa
     1981 gcggcatgct ctgcgagggc atggagcact tcaccgccgc ggacgtggag gatatccaaa
     2041 gccgcggtct cgtctgccgt ctcatggcgc gcggcagccg cgccggggag agcgtttcgg
     2101 cgtatgtgga gccggtgctc ttcccccgtg ccgcgccgga gagctcggtt ttcacgaata
     2161 acaacatggc gcgctatacc ggcaagcgct gcggctccat cgtgctgatg gggcagggcg
     2221 caggccgctt ccccaccgct tcggcggtgc tgcgcgatct tgccgcggcg cagcagggcg
     2281 cgcgctccat gttcccggaa aactgccgcg ccgcggaagc cgataaccgc gaagcggtgc
     2341 attcctacta cattcgcacc ggcggctttg ccgcggttcg gcttccgctc cggtcggtgc
     2401 tcgcgcaggg cgaagttgtc cgcgccgtta ccgagccgat gagcgcagag aaaatgcacg
     2461 cgctcgcgca gaacatccgc aaggacggaa acgatctgtt cttcgcggcg ctggaggaag
     2521 aactatgctg aaagtatgca aattcggcgg ctcgtccgtc gcaggggcgg agcagttccg
     2581 caaggtgcgc gacattgtcc tttcggacga ttcgcgccgc gtcgtcgtca tctcggccgc
     2641 cggaaagcgc acgagcgacg atcacaagct caccgacctt ctctatctct gccacgcgca
     2701 tctgacctac ggcgtctcgt gcgccgacat tctcgccgcg atccgttccc ggttcgtctc
     2761 catccgcgac gagctgggtc tttcctaccg cgtggaggac gaattcgacg cttacgccgc
     2821 gacgctctcg cgtgacacga gtgtggacga gctcgtctcc cgcggcgaat acttcaccgc
     2881 gcggctcatg gccgaaacgc tcggctacgc cttcgtggac gccgccgact gcgtgttctt
     2941 cggctttgac ggccagtttg accgcgcccg ccccgacgcg gccatcgccg acgcgctcga
     3001 aaagaacggg aagatcgtcc tccccggctt ctacggccgc gtgccgaccg ggaagatcaa
     3061 ggtgatgaca cgcggcggca gcgacatcac cggcgcgctc gccgcagccg cggtgaacgc
     3121 ggacgtatac gaaaactgga cggacgtttc cggcatattg atggccgatc cgcgcattgt
     3181 ggagaatccc cggcccattc cccgcatcac ctacgcggag ctgcgcgaga tggcgttcat
     3241 gggcgcttcg gtgctgcacg aggaatccat tctgccggtc aaggaaaaag gcatttccct
     3301 gaacatccgc aacacgaacc ggccggacga gcccggcacg ttcatcgtgg agcacatcga
     3361 aagcgaaagc gagcttgagc gcccgatcac cggcattgcc gggcggcgca acttcacgat
     3421 cctcacggtg ctcaagcgca acatgaacgc ctccacgacg ctctgcaacg cgctggagat
     3481 cctcgaccgc taccacgcaa gtgtggagca catcacgctt gggctggaca gcttcgcgct
     3541 cgtcgtttcg tccgctgcgc tggaaaatac gctctatgat gtcatcgccg acattcagaa
     3601 gacctgccgc ccggacgatg tgcgcatgca ggacggcatt gcgctcgtcg ctgccgtggg
     3661 ccgcaaaatg cccgcccggc ccggcacctc cggcaagctc ttccaggcgc tcggcgagca
     3721 cgggctgaat atccgcacca tcgcccaggg cgcggacgag ctttcgatca tcgtgggcgt
     3781 ggaaaactgt gattttgaaa aggcgatccg cgtattgtat gacgggttca ccggctgaca
     3841 gaaaggagat cctcttatga aaaattgcaa tatcgccatt ctcggcgcga ccggcgcggt
     3901 cggccgcgag atgctcaacg tactggaaga atacgatatt cccgtttcgc gtctcaagct
     3961 cctctcgagc gcgcgcagcg cggggagcaa aattcccttc cgcggggagg acctcaccgt
     4021 ggaagaagcg acggacgcct cgttcgaggg catggacttt gtcctcggcg cggtaaagag
     4081 cggcatgtcc cgcgcgtttg ccccggccat cgtgaaaagc ggcgcggtat acatcgacaa
     4141 cagctccgcc ttccgcatgg atccggccat cccgctcgtc gtgccggaga tcaacggtga
     4201 ggacgcgaag agcaattccg gcatcattgc caatcccaac tgctccacga tcatcaccgc
     4261 gatggccgtc ggcgcgctca accgcctctc gccgatcgag agcatggtcg cgtgcacgta
     4321 tcaggccgta tccggcgccg gacagggcgg acttgaggaa ctcgagcacc agatggacgc
     4381 gctcgcccgc ggtgaaaagt gcgcggcgaa ggtattcccc acgcagatcg cgctcaacgt
     4441 catcccgttc atcggcagca tgctcgataa tctctatacc aacgaagaaa tgaaaatgca
     4501 gaacgagggg cgcaagatcc tgcatctccc cgggctgcgt gttacctgca cctgcgtgcg
     4561 cgttcccgtt atgcgctcgc actccatcgc cgtcacggtg cggacaaagg agaagatcac
     4621 cgttgccgag gccaacgaag ccatccgcgc cttccccggc tgccgtctga tcgaggacta
     4681 cgagggccgg tgctacccca ccccgctcga cacaagcggg caggacctcg tctgggtcgg
     4741 ccgcgtgcgc gaggatctca cgcaggaaaa cgctctcacg ctctggtgct gcggcgatca
     4801 gatccgcaag ggcgcggcga gcaacgccgt gcagatcctg aaataccttc tgtaattctt
     4861 tcatttatat gcagccatcc ggcagtcttc ccttgggaag gctgccggat attttttcgc
     4921 tcgttgcata caaatatgca caaagcgttt ggataatatt ccgtattcgc cgtatattat
     4981 ttataataac gcaaatattc cgaatttttt ctcattcttg tgttgactcg cgtggataag
     5041 tatgctaata ttcgcaccat cttaaataaa aatccatctc gtatcggagg catacatcca
     5101 tgaaaaagaa accatttgtc aaagtccttt ctctcgctct cgccgctatt acgcttttcg
     5161 tgctctgcgc ctgcggcaca gcagcgtcgt ctcccgccgc agggccggta tcggacgcag
     5221 ccccaaccga agcaagcgcc gcatccaacg gcgttcttga cgagatcaag gcctccggcg
     5281 agctgcatat caccctgtcc cccgattttg ccccaatgga gtttatcgat tcctccaagg
     5341 acgggcagga gcagtatgta gggctggacg ttttccttgc caagtacatc gccgactata
     5401 ttggcgtaaa gcttgtcatt gagccgatga gctttgatgc ctgccagacc gccgtgtata
     5461 ccgcttctgt tccgatgtcc gtttccggct acgtctggac cgaggagcga gccgaaagct
     5521 atgagatctc cgattattac tatgatggcg ataacaaaat tgagccggtc attctgatcc
     5581 gtaccgcgga cgcggaaaag tacacgtctt ccgaagattt taacggtgtt gacatcggcg
     5641 cacaaaacgc ctctctgcag atgcagcttg tcacttctca gcttcccgac gccaatcccg
     5701 tagccgtcgg tgatgtcggg accggcgttc tcgagctgca gaacggcagc attgaagctc
     5761 tcgccgtggc caagggcaac gccgagatca tcatggacgc caacccggat ttggtgcttt
     5821 gtccgtggga attcaccatc acggaggaga cggaagcctt tgtcattctc atcaccaagg
     5881 gcgagaccgc gctgctgaac gttgtcaacg aagcgctggc aaaagcctac agcgaaggaa
     5941 tgtacggcgc atggcacgat gaagctgttg cgcttgccaa agtcgtgaac ggtacagaag
     6001 aataaagata aatatgttta gcaaaaagtg aaaattgagg aaaagaaaat gaaaaagtat
     6061 tcaaaggttc tctcgcttgc tctcgcattt gtcatgctct tcgcgctctg cgcctgcggc
     6121 gcggcggaga ctcccgccgc ggagcccacg gcagcccnnn nnnnnnnccg ccgacgctcc
     6181 cgcatccgaa gcccccgccg agaccgaagc tcccgccgaa tcgcagggcg tgctcgatca
     6241 gatcaaggcc gacggtgttc tctacgtcac cctctccccc gactttgccc cgatggagtt
     6301 tgtcgattcc tccaaggacg gacaggagca gtacgtcggc tttgacgtga cgctcgccaa
     6361 gtacatcgcc gagtacatcg gcgtggatct tgtcattgag ccgatgagct ttgacgcctg
     6421 ccagaccgcc gtttacaccg gctccgtgcc gatgtccatt tccggctact cctggacgga
     6481 ggagcgcgcc gagaactacg agatctccga ttactactac gccggcgata acgagaccga
     6541 gcaggtcatc ctcatcaaaa agaccgacgc cgacaagtac acctccgccg aggacttctc
     6601 cggcgtggat gtcggcgcgc agaacgcctc gctccagatg aatctcctca cctcccagct
     6661 gcccgacgcg aaccccatca ccatcggcga tctcggcgtt ggcgtgctgg agctgcagaa
     6721 cggcagcatc gaggcgctgg ccgttgcgaa gggcaacgcc gaaatgatcc ttgattccaa
     6781 ccccgatctt atgatctgcc cctgggaatt tgaagttgcc gccgagtatg aggcgaacat
     6841 cattctcatc accaagggcg agaccgcgct gctgaacacc gtcaacgaag ctctcgccat
     6901 ggcctatgcc gacggactgt acggcacatg gtacgaagac gccgtcgcgc ttgccaagtc
     6961 ggagaacgcc gccgaagtaa gcgtcgaaga ctgattgcaa ataacaagct gcccgccgtg
     7021 ggggatctct ccggcgggcg gaggggaagg atccgtccat gagtttcata aagtttgaaa
     7081 acatcgtcaa gctgttcacc aaatactggg aaaagtttct ggtgaccggc gtatcgtata
     7141 cgctactgct cgccgcgatc acggtggcgt tcggcgcggc gatcgcctgc ctgctcgcgg
     7201 gaatgaagct ttccaggatc gcgccgctgc gctggctttc cacagcttac atcgagatca
     7261 tccgcggcac gccgatgctt ctgcagctct atttctttta ctttgccctg cccgcgctca
     7321 ttccggcgct caataagcag aaatttctct gcatcgccat cgcgctcgtc tgcaacagcg
     7381 ccgcctacgt ctccgaggtc atccgctcgg gcattcagtc ggtagacgcc gggcagagcg
     7441 aggcggccat gagtctcggc atgaattcgt tccagcgcac ctggtacatt ctcgtgccgc
     7501 aggcgctcaa aacgatcctt cccgcgctcg gcaacgagtt tatcatgatc gtgaaggaca
     7561 cctcgctcgc gtccacgttc tttatcggcg atctcatgac acagtatctc atcgtgcgcg
     7621 gcgcaagcta tctgcctctg gagccgctcg tgatcgtcgg cgtcatttat tttattctga
     7681 cgttcctgct cagcaagctg ttcggattct ttgaaaggag gatgagccgt gcagaccggt
     7741 gaaatgctca ttgaggtcaa agacctctgc aaacacttcc gcgacggtac ggtcaaagcg
     7801 ctcgatggtg tgagtctcac gatctgcaag ggcgacgtga tcgtcatcat cgggccctcc
     7861 ggcagcggca agtctacgtt cctgcgctcg ctcaatctgc tcgaaacgcc gacctccggc
     7921 agcgtgatcg tcgaaggaac ggatatcacg aagaagaaaa acgcgcaggg aaagaagatc
     7981 gatctcgaca ctcaccgccg gaagatgggc atggtgttcc agcacttcaa tcttttcccg
     8041 cataaaacgg tgctcgaaaa tctgaccatc tcccccattc ttgtaaaaaa agcagacaag
     8101 gccgcggcaa aggcgaaggc cgtggagctt ttggagcgcg tcggacttgc cgaccgcgcc
     8161 gacgcttacc cctcgcagct ctccggcggg cagaagcagc gcgtcgccat cgtgcgcgcg
     8221 ctcgccatgg agccggacgt gatgctcttt gacgaaccga cgagcgcgct tgaccccgaa
     8281 atggtcggtg aggtgctcga cgttatgaag cagctcgccc gcgacggcat gacgatggtc
     8341 gtcgtaacgc acgaaatggg ctttgcccgc gaggtcggca accgcgtcgt attcatggac
     8401 gaggggcgca tcgtggagca gggcacgccg gaagacatct tccagcaccc gcaaagcgcc
     8461 cgtttgcagg attttctttc caaggtgttg tgatatgaac gaaatcaaaa aaaccgcgct
     8521 gcatacgatc gacgcgcacg caaatacttt caccgcgatc tccgacgcga tctgggacga
     8581 accggagctt tcgctcaagg aattcaaggc cgccgcgctc tatacggacg cgctggaaaa
     8641 gcttggcttc acggtgcaga agaacctctg cggcattgaa acggcgtttt ccggctccta
     8701 cggctccggc catccggtga tcggcatcct cggcgagttt gacgcgctct ccggcctctc
     8761 gcagcaaagc ggcgttgccg aagcgcagag cgtaaccccc ggcggaaacg gccacggctg
     8821 cggccacaat ctgctcggcg ccggttcgct cgccgccgcc gcggcggtaa aggagtatct
     8881 cgccgcgagc ggaaagcccg gcaccgtcgt tttcttcggc tgccccggcg aggagggcgg
     8941 cgcggccaag gcgtatatgg cgcgtgaagg gctctggtac gggctcgacg ccgcgctcac
     9001 ctggcacccg tccgacacga acgaggtatc caccggcacg aacaactcct gcattcagat
     9061 gctctataaa tttcacgcca tcgccgcgca cgccgccgga gacccgcaca acgggcgcag
     9121 cgcgctcgac gccgtggagc ttatgaaaat cggcgtacag tttctgcgcg agcacatgac
     9181 cgacgactgc cgcatccact atgccatcac ggacgcgggc ggcgtgtcgc cgaacgtcgt
     9241 gcaggcgaac gcctcggtgc tctacatggt ccgcgccaac aaggtcgccg attccgtaaa
     9301 gctccagaag cgcgtggacg ccatcgccga gggcgcggcg cttatgaccg ggacaagcta
     9361 cgagcgcgta ttcatcgacg gcacggcgga aacgctgccg aacttcacgc ttgaagcgct
     9421 gctgcacgcc tcgttcgccg aaaccggcgt gccgcagtat acggcggagg agcttgcctt
     9481 tgcctcgtcg ctctgccgga cgtttccgcc gcagacgcgc gctcccggca tcggcgcaaa
     9541 gtttgacagc gaaatcgcca aagaatcccg tgcgctttca gagaatatga ccaagcccat
     9601 gaacgacttc ctcctccctc tctactccgg cgagggcttt ctccccggca gcacggacgt
     9661 cggcgacgtg agctggcaga cgccgacggc gcaggcgctt atcgccgcgt ggccggcggg
     9721 cgtgccgggc cattcgtggc aggtcgttgc ctgcgggaaa acatcccttg cacacaaggc
     9781 gatcctctgc gccggaaaaa ctctggcaac cgccgccatc gcgctcatcg acgatcccgc
     9841 gctgctgcaa aaggcccgcg aggaatacgc cgggcgagcc ggatccggct acgtctgccc
     9901 catcgagccg gacgccgtgc cgatcgcgat ctgatatccc aaagcatttc ccgacaaatg
     9961 tccgccggtt ctctgtccgc cggannnnnn nnnnacattt gtttttattc attgtcaaaa
    10021 tccacagtat attcattttt attcgtgttt ccccgttcac gattcataaa tttcataaac
    10081 cactgaattt tattcgtaaa caggcttgac tttgcgagca gccggtgtta atatccgtcc
    10141 atactaaata ataattcatt tggaggaaga aacaatgaat accgttccca agtacatgtc
    10201 caccgccgtc accgtcattc agtgcgcaac gattgttgcc ctgatcgcga tcttcgcccc
    10261 gctgttcatg taatgcactg aaggtcgtat aaaaaggcct tgcacacaaa accccgtctg
    10321 ctttcaaaga ggcagacggg gtttttcaat attctgacga gtcaatgatt cgctgtaaaa
    10381 ccggcacagc tcagtttttg agacggatca gcccgcagcg gtcagttcat cccaagtgat
    10441 aaaactgccg cacgccgtgg tttttgatcc atgattcagc aaaatgggcc gctgtgccgc
    10501 atcgcccagc agcgagaaat ctccagtaac agaatagttt ccgccggaag agtttgcctg
    10561 aatccgaatc gtgtcgttat ctatatactc ccatttcata gtggaatact tggaattgct
    10621 aaggttttca aatgaatcaa atacgattcc ctctccattc tccccaaaga ccataaactg
    10681 atttgcaatg acccatatcc cgttatcttg tgccaaaagg tacttctcga cttccgcgcg
    10741 gacctccgcg ccagcagctt cgtagtcatc ccgtctcaca tagggatatc ttttcgtgtc
    10801 ggtaacaagt tctgttcgtc ccccgagctc caaccgcaga gtaacatttt cctccacgcc
    10861 atcttccgaa acgataatcg ccatgttatt ctcatcttcg ctccgcgtgt aggtgcaggg
    10921 aacattgtca tgtgtccctt ctccggtaca ctcgaaaaca tagatgtcac cgtctgcgga
    10981 atttatccac tcatagctga acggtcggtt actggcagta aaagccagaa aatcgtattt
    11041 ttctctggcg tctctcaaat cagagatatt ctccacgctc tctttttcct catcggagag
    11101 tgcatcatac attttttccg cctcttcaat ggcttcaaaa ctaagcaaag atatttttcc
    11161 aaaagaagaa attgcctctt ccacgctttg aacctgcctg ctcttcccgc aaccgcataa
    11221 agacacgcat aaagccagta ctaaaatcgc agataatctc tttttcattc catttctcct
    11281 ccttaaaata gggaccgata ttggtcatta ttgctatatc acaaattcgg ttatcatgtc
    11341 aagaggtaag ggagggactt ggcatgattt cgtatgataa attgtggaat accatgaaga
    11401 aacgcaagat ttcacaatat gcgctcaaga aaaaatacgg catcagcccc ggacagatta
    11461 cccgcctaaa gcggaacgaa agtgtcagca cgcacacaat tgaaatgttc tgcaaaatac
    11521 tttcctgtga tatcagcgat ataatgcagt atataccgga tgaaacggac gagaaataag
    11581 gccctatttt tgttttgata agactagaac gcaaccctcc aaagcagcat tcgccggggg
    11641 ggctgcgcca tatgtgttgc cacagggagc ttttcttaac agaaaaacgc tgtgcgaact
    11701 ttcgttcgca cagcgttttc ttttctatcg gtttaccggc agacgagctc gtcgttttcg
    11761 acgtccaccg tgagcgtgct gcccgcggcg atatcgccgg agaggatggc tctcgccagc
    11821 agcgtttcca cgctgctctg gagataccgg cgcagcggac gcgcgccgta gagcggatcg
    11881 tagccgcgct cgatgataag ctccttcgcc ttgtcggtga tgcagagctt gagcgagcgg
    11941 tcggccaggc gcttcttgag cccttcgacc atgatatcga tgataccggt gaggttgtcc
    12001 ttcgtgagcg gcttgaagag gatcgtctca tcgagacggt tcaaaaactc cggccggaac
    12061 gcgcggtgca gctcacccat gacggcttcc ttcgcggaat cggtgatgtt gccctccata
    12121 tcgatgcctt cgagcagata ctggctgccg aggttcgagg taaggatgat gatcgtgttc
    12181 ttgaagtcta cggtgcggcc ctgcgaatcg gtgatgcggc cgtcgtcgag aatttgcagc
    12241 aggatgttga acacatccgg gtgcgccttt tcgatctcgt cgaacagcac aacgctgtag
    12301 ggtttgcgcc gcacggcctc ggtgagctgg ccgccctcgt cgtagccgac atatcccgga
    12361 ggcgcgccga tcagacgcga gacggagaac ttctccatat actcggtcat atcgatgcgc
    12421 acgatgttgt gctcgtcgtc aaagaggcat tcggcaaggg acttcgcaag ctcggtcttg
    12481 ccgacacccg tggggccgag gaacaggaag ctgccgatcg ggcggttcgg gtcggcgatg
    12541 ccggcgcggg agcggagaat ggcctccgtg accttctgca cagcctcgtc ctgaccgacg
    12601 acacggcggt ggagataatc atccagatgc aggagctttt cgcgctcgcc ctcgacaagg
    12661 cgcgccacgg gaatacccgt ccagcgcgcg acgatggcgg cgatttcctc ctcggtaacg
    12721 gtgtcgtgca ccatggagtt ctgcttcttc tcctccgagg ctttcacggc ctcggcgagc
    12781 tttttctcca gcgcgggcag ctcggaatat ttgagccgag ccgccgtttc atactcgtag
    12841 cgcagctgcg cgttttcgat gtccgcgtga agcttttcga tctcgccctt gagctttttg
    12901 acctcgtcca cgctgttctt ttcgctttcc cagcgcgcct tcatctcatt gaacttgtcc
    12961 ttgagattgg cgagctcttc gcgcagcttg gcgagacgat cctggctgag ctggtcgtcc
    13021 tccttcttga gcgccatttc ctcgatttcg agctgcatga tcttgcggcg gatgccgtcg
    13081 agctcctcag gcatggaatc gatctccgtg cggatctggg cgcacgcctc atccacaagg
    13141 tcaatggcct tatcggggag gaaacggtcg gtgatgtagc ggttggagag cgtcgcggca
    13201 gcgacaagcg cgttatcatg gatacgcacg ccgtgataga cctcgtagcg ctccttcaga
    13261 ccgcggagaa tggagatcgt gtcctcgacc gtcggctcat ccacctgcac gggctggaag
    13321 cggcgctcga gcgcagcgtc cttttcgata tacttgcggt actcgtcgag cgtcgtggcg
    13381 ccgatgcagt gcagctcgcc gcgggcgagc atcggcttca agaggttgcc ggcgtccatg
    13441 ctgccctcgg tcttgcccgc gccgacgatg gtgt
//
LOCUS       DAWAAE010000044        13374 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2071_length_13374_cov_3.793378, whole genome shotgun sequence.
ACCESSION   DAWAAE010000044 DAWAAE010000000
VERSION     DAWAAE010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2071_length_13374_cov_3.793378"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4192..4201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2789 a         4056 c         4170 g         2349 t
ORIGIN      
        1 gccgttcatc tcataggtgc cgtactgctc aatgagccgc cccagccgca gcacaccggc
       61 gatgagcagc aatacaccgg cggcaaagca cgcgaagaac acgccctgca gcccatgcgc
      121 cgccacgatg ccgacgagca cggcgctcat agcgccggtc ggaccggaga tctggaacga
      181 cgcgccgccg agcagcgcga tcacgatacc ggcgacgatg gccgtaacaa ggcccgccgc
      241 ggcggaagcg ccggacgaaa cgccgaacgc gagcgcgagc ggcagcgcca cggcgcacac
      301 cgtgagaccg gcgagaatgt ccttcaccag cttcgcggcg ttatagccgg aaaactctct
      361 ctttactccg gcaaaaaacg aagaaaacac gatgattcct cccccaaaaa aagacgtacc
      421 tgcccactat acgccccaaa ccgcctgttc gcaatagaaa aaataaacat tctgtacttt
      481 tttgaaaaat aatagcaaaa atgttggaca aaataggggg atcatataaa gaagatatga
      541 atgcagccat ccgccgcggg aacatacggc atgggcattt ccggcggcag cgactgccac
      601 ggcacacgca aaacgcacat ctccatgggc acgggctacg gcaatctcgc aataccttac
      661 tcggcgcttg aggggctcgg aaagctgcga tgaaaaactt cccctgtgta aatgttcccc
      721 gctttcccgg aaggaaagcg gggaacattt ttatgcgccg aagaggggca gcgcttcgat
      781 ggcgcgggcg atgcgttcgt gggtaagatg taccgctgtg ggcgcgtcac cgttcaaaag
      841 ggcggtataa agcgcgagct tcgattcata cattttctgt atgccgctgc gcttgcagtc
      901 gatcgtccag agatacacgc tctgcgggcg gaaggagtgg tataaaagcg gcatgagcac
      961 gttgccggag agcaccgcaa gttcgtggtg aaaaaggaaa acgttctccg cggcctcgtc
     1021 gctgtcgcgc gcggtgcgga tgctctccaa aaccggcgcg agcgcttcga gcgatgcggt
     1081 gtccgagcgc tcgacgacga gacgcatgca catacactcc atcgcgtcgc gcgtttcgag
     1141 cagggagcgc acctcgttct ggcgcagcgc gccgccgttg tagcgcatga tcgccacgag
     1201 cgtttccatc gtgccgcgcc gccggtaatc gcagacgtac acgccctgcc gggctttgat
     1261 ctccacaaag ccgagctttt cgagctctac cagcccggcc gttacgacgg tgcgcgaaac
     1321 gcccatcttc gccgccagct cacgcgccgg gggcagctgt tcgccgaagg caagctcgcc
     1381 ggagaggatc atgttttcga tctgctgcac gaaaagatcg gtcagcgaaa gcgcggtgat
     1441 cttgctgtag cccatagccg tttcctccgg tggatgcgcg tggaaatacc acgtttattt
     1501 atttcaaatt tacccgcaat atacgcaaaa gtcaatcgcc ggttttttgt aaaccgatga
     1561 aatctcctgg cggcgcttga cgagcgggcg gcgcggtata tattcttata agtgaaatcg
     1621 cgtggtaata ccacgaaagg gcaagcgcga aaataagtaa ggaggacaag accatgcaaa
     1681 aatacgatgc cgtagtcatc ggcgcgggca acggcggtct cgcggcggcc tgccggatgg
     1741 cgaagggcgg caaaaagacg cttcttgtcg agcggcacaa ccttccgggc ggctgcgctt
     1801 cgagcttccg ccggggacgc tttgagtttg agaccgcgct gcacgagctc tgcgaatggg
     1861 gcggcaagga aaatcccggc ggctgccgca aaacgtgtga ggagttcggg ctggatatcc
     1921 cgtggcacat ggtaccggac aacttccgcg tgatcaccac cgcctccgac ggcaagaccc
     1981 acatcgatgc gacgcttccc tgcaccgtga aggaatttgt caacgaaatg gagcggctcg
     2041 tccccggctg ccgcaagtcg atccaggatc tttttgatct cggcaccgag ttccacgaag
     2101 cgggcgacta ttcgctcagc gtcgcgggaa agaccgaccc gaaatacatg caggagcact
     2161 tccccaactt cctgcgtctc ggcagctaca cggtgaacga ggtgttccgc gccatgaaaa
     2221 tgccgaagct cgcgcaggac atcatgggga cctactgggg ctatctcggc gtggatatgg
     2281 accacctctc cttcctgcaa tacgtgaaca tgctctggct gtacgtcaac cacggcgcat
     2341 ggatcccgga taagacgagc aacgagctga cgaccggcct tctcgaatgc ttccggaaga
     2401 tgggcggcga gatatggatg aactgcaccg ccacggagat cctctttgat gagaataagg
     2461 ccgtccgcgg cgtgaaaacg acgtgcggcg aggtggaaac gcgctatatc ctctgcaaca
     2521 tgaatcagaa catggtttac gccacgatgt gtcccccgga ggtcgtgccc gagcgtatgg
     2581 tgaagctcgg caacgcgcgc acctattcgg cgcgcatgtt cgtcgtgtat ctcggactgg
     2641 acagatcggc ggaggagatc ggcattcacg actatacgat cttccttccg acctcggcgg
     2701 acagcgcgaa ggagtatgag acgggcaagc gcatggagac caactgcaac caggtcatgg
     2761 tctgctacaa cgttgtgaac ccggcgttct cgccggaggg gacgtgcgta atgtcactca
     2821 cgagcacgtt catggccgac gtctgggcgg acgtggagca ggacgactac gtaaagacca
     2881 agaccgactt tgccgaaaaa ctcatccggc tctttgaaga gcgcaccggc atcaccgtca
     2941 cgccgtacat cgaagagatc gagatcgcga cgccgtggac gatgtgcaac tatgtaaata
     3001 ctccggaggg ctcggcgtac ggctttgagc tgaaggactg ggacaatatg atgccgcgca
     3061 tgatgatgat gggcaccgag ttcccggtca aggggctgaa gttcgtcggc gcggcgtcca
     3121 tccgcggcga cggctacaac tccgcgatct tcagcggcga cacgctcgca aaaaagaccc
     3181 ttgccgaaat gaaagcagag gaggcttgag tgacatggcg gcaaactgca actataaagt
     3241 gaagctgatc ggggcgctcg acatgctccg gttcaagaat atgcccaagg tccgcgaggc
     3301 ggcgatccag gcggccccgg cgacgcctct gcccaaaacg ttcacgacga acgaaaaggc
     3361 gaaggtccgt catcccgaga tgcagcgtgt cgtcgttgcg gagatccggc ccaacggccc
     3421 ggacgtcaag acgatcgtgc tcaagtccga ggacggcgcg ccgctcgcgc cgttccgcgc
     3481 cggacagtac gtgagcgtga ccgcgcacat cggcgaaacg gtcacgaccc gcgcctactc
     3541 gctcagcggc agccccgcat gggcgaagcg cggagaatac aacatcgccg tacggcgcga
     3601 cccgaacggc tttgtttccc cgtgggtaca ggatacgtgg caggtcgggc agaagctcac
     3661 gatctccggg ccgcagggcc agctctacta tgagcgcctg cgcgacgcga agaaggtcgt
     3721 ggcgctcgcc ggcggcgtcg gcatcacgcc gttcatggcc atggcccgtg ctctgcgcga
     3781 cggtctggag gattttgatc tcaccataat ttacggctcg cgcaccgagg cgggcatcgt
     3841 ctataaaaag gaactggagg aggtcgccgc ggcgtgcgat aaggtgcacg tcgtccacgt
     3901 tctctccgac gaggaaaagg aaggctacga gcacggcttc atcaccgcgg agctcatcaa
     3961 aaaatacggc ggcgcggagt acagcatctt catgtgcggc ccgcagggga tgtacaatta
     4021 ccttgatacc cagatcgagc cgctcggcct gcgccgcaag tatgtgcgcc gcgagctttt
     4081 cggcgcgatc aaaaatccgt gggatcagcc ggattatccc gccggtattc gaggcaaaac
     4141 gtttaaaatg aagctcatcc agtgcggaaa ggaatacgag atccccgtct cnnnnnnnnn
     4201 nccgccgagc gcgccggaat ctgcgtgccg gaacgctgcc gcagcggtga gtgcggctgg
     4261 tgccgctcgc gcctgctctc cggcagcgtg tatgtccccg cgcgcaccga cggccgccgc
     4321 cgcgcagacg ttgcgtttgg gtacatccac ccctgcgcca gctttgccat gtcggatctc
     4381 gtgctggacg tgccgaacgg cacgctccgg taaagcgagc ggcaggaata ccgaaaatgt
     4441 cctcccgaaa ttctcggggg gacatttttt gcccgcgtca gctcttttgg cgcttctgcc
     4501 ggaaaagagg caaggaaacc cttgcggcca tgggtagaac gcggtatgat ataaaataaa
     4561 gtgaagccgc ggcgggaccg cggctgcgga ggaagattat gcgctattac cttggattgg
     4621 acaacggcgg cacgaacacg aaggcagccc tttttacccc ggacggcacg caggtggccg
     4681 tggagagcgt tgccaccgcc gcgctcacgc ccgcgcccgg ctttgtggag cgggatatgg
     4741 aggacatgtg gcgcgataac tgcgccgtca tccgcggcgt gctcgaaaag agcggcgtgc
     4801 gagccgaaga gatcgcgggc gtcgggctct gcggccacgg caaggggctg tatctctggg
     4861 gcaaagacgg taagagcgcg cgccgcggca tcctctcggc ggacaaccgc gcgtggaaat
     4921 acccggtcga atggcgcaag gacggcacgg aggaacgggc gttcgccctc tcctgccagc
     4981 acgtcatggc gtgccagccg gtgagcctgc tcgcatggct gcgcgataac gagccggaga
     5041 cgatgaaaaa catccgctgg gtgtttgagt gcaaggacta tgtgcgcttc cgtctgaccg
     5101 gcgaggcgcg cgccgagatc accgattatt ccggcagcgg actcatggac ctgcatacga
     5161 agagcttctc gaaggagctc ctctccctct tcggcatcga gagcgtgtgg gatgcgctgc
     5221 cgccgctctg cgcctcgctc gatatcgcgg ggcatattac gccggaggcc gccgcgctca
     5281 cgggactgct gcccggcacg ccggtcatcg gcggtatgtt cgatatcgac gcctgcgctc
     5341 tcgccgtgaa cgtcacggac gagacgaaca tctgcatgat cgccggaacg tggagcatca
     5401 acgaataccc gcgccgcgcg cccatcacgg acggctcggt gctgatgaat tccctcttct
     5461 gcctgccggg gtactacctc atcgaggaat cgagccccac ctccgcgggc aacaaccagt
     5521 ggttcatcga ccggctcatg cccgagctca aggccgaggc gaagacggcg ggaaagagca
     5581 tttacgatat tctgaacgac tgggtcgatt cgtttgcgcc cggcgatttt gtgccggtgt
     5641 tcctgccgtt tctgacggcg agcaacgtcg atccgcgcgc ccgcgcagcg ttcgtcgggc
     5701 tgaacgcctc gcactcgcgg cgggagatgg cccgcgcggt gtacgagggc atcacgttct
     5761 cgcaccggta ccatctcgac cgcctgatgc gctcgcggcc cgaccggagc gggacgatcc
     5821 gtctcgccgg aggcgcggcg cgttcaaagg tgtggacgca gatgttcgcg gacgtgatgg
     5881 atatgcccgt cgagaccgtt tccgcggagg agaccggcgc gctcggctgc tgcgtggcgg
     5941 tcgcggcggc gctcggcgat tataaagacg ccgcggatgc cgcggcgcac atgtgccgga
     6001 gcgtgagccg cgttctgccc cggccggacg ccgcgaaggt ctacgaggaa aaatacgcgc
     6061 tgtatctcaa aaccatcgag tgcctgaacg ccctctggcc ggatatgcag cggtttatgg
     6121 aggaacatca tgcttgaaaa gctgaaggaa agagtctgcg aacagaatct ccagctggta
     6181 aaatacggcc tcgtcgtgct cacatggggc aacgtctccg ccctctcgga ggacggaaaa
     6241 tacgttgtca tcaagccgag cggcgtatcg tatgacaata tgaaggccgg cgatatggtc
     6301 gtcacggacc gcatgggcaa cattgtggaa ggcgatctcc gcccgtccac cgaccttgcg
     6361 acgcatctcg cgctttacga tgccttcccc ggcatcggcg gcgcggtgca cacgcattcg
     6421 cgctacgccg tcgccgcggc gcaggcgggg ctggatctcc cctgctacgg cacgacccac
     6481 gccgactact tctacggcgc cgtcccctgt gcccgggagc ttacggcgga ggagatcgag
     6541 gaagcctacg agcgcaacac cggcctcgtc atcgccgaga cgttccgcgc tcggggcatt
     6601 gatccgctgg cagttccggc ggtgctcgtg aagagccacg gcccgttcac ctggggcaag
     6661 acgccggaaa aggccgtgga aaacgcgctc gtgctcgacg agtgcgcgca tatggcgctg
     6721 ctcacgcggc tctttgaccc ctccgccgcc cccgcggcgc agtgcattca ggacaagcac
     6781 tatttccgta agcatggcgc gaacgcctac tacggacaga aataaactac aaaagggagt
     6841 gtcgctatga tccgcaagcc ccgcatactt gttgtcggca tcttcgtcat ggacctgatc
     6901 gcctccacga agcaggcccc aaaccgcggc gagaccgtga tcggtctcaa cttccggaca
     6961 gctccgggcg gaaagggcgc gaatcaggcc gtgcagtgcg cgcgcctcgg cgcggacgtc
     7021 acgatggtcg gccgtgtcgg taacgacgcc tatggccgcg agaccgtcgc ggcggcggcc
     7081 gcctccggcg tggatgtgtc ccgcgtcgtt gtggacgccg aaaatccgac cggcgtttcc
     7141 gccatcacgc tcgagatcac cgaaaccggc gcgcacaacc gcatcgtcgt ctgccccggc
     7201 gcgaacggcg caatgaccgt ggaggagatt tcctggatca gggacgaggt aaagaattac
     7261 gatctcgtca tgctgcagtt cgagctgccg atgttcatca acgaggccgt ggcgcgctgg
     7321 gcgcacgcgg ccggcgtgcc ggtcatggtg aaccccgctc ccgccgcgcc cgtttcgccg
     7381 gagctcttcc gctgcgccac gttcttctcc ccgaacgaac acgaggccgc cgcgatggcg
     7441 gggcatccca tccgcgtgga cgatgcgggc gcgaacgagg acgatctgcg cgccgtggcg
     7501 gatattttcc gcgaaaaggg cgtggagaat ctcattgtga cgcttggcgg gaacggctcc
     7561 gttgcggtga acaaagacgg cgtcgtgcgc gtgcagagcg taaaaatgcc cgttgtggcg
     7621 gacaccacgg cggcgggcga ttcattcgtt gcgtcgttca gcacgctctg gtgtgcgggg
     7681 cttccggcgc ggcaggcgct ggcgtttgcg agccacgccg cggccatcac cgtttcgcgt
     7741 cccggcgcga tcccgagcct cccgacgctc gcggaggtaa cggcgctgct gcgcgagcgc
     7801 ggctatgagg atatggatta cgatcttttg gcacagttgg aggagaaaag caaatgaaaa
     7861 acgaaggaaa aaccgcgctc gcgcatctga ccgcgtccgc ggaaaaggaa ttctgcgcgt
     7921 ttctcgcgca gcagacgccg gaggcatacg aagacgcggt cgcgctcatt gaggaatgcc
     7981 gcgccgcggg acaccgcgtc catgtgaccg gcatcggaaa gcccggccat gtggcggggt
     8041 acggagcctc gctcctctct tccaccggca cgccgtccta cttcctccac ggcacggagg
     8101 cggtgcacgg ctcctgcggc cagctggagg aaggcgacgt tgtcattgcc atctccaact
     8161 ccggcgagac ggtggagctc aaggccacgg tgctcgccgt gaagaacaac ggctgccgcg
     8221 tcatcggcat caccggcaat gcggaaagct ggctcgcaaa ggagagcgac gcgctgctgc
     8281 tcgcgcatgt agacgaagag ggcggcccgc tcaaccgcgc gccgcgcaac tccgtgctcg
     8341 cggagatgtt cgtgctgcag gcgctctccg ccatattgca ggcggacgtc gcgcagacgc
     8401 ccgcgcagta tgtccgccgc caccccggcg gcgcgcttgg caagatccgc gataacgagc
     8461 gctgaacacg ccggaatacc gcacagtgcg ggagaaagag gaagagacat gaaaaacgtg
     8521 acatcggata aacgcaatct ctggatgttc cccctcggca cggtcggacg ggatatgatc
     8581 tacaacctgt tcaccaactt cattttcaca tatatcctgt ttacacgcca tctcaccgcc
     8641 gcgcagctcg gcgcagtgac ggcgatcatg gtcggcgcgc gtgtgttcga cgcgctcaac
     8701 gaccccgtca tgggcaacat catcgagcgc acccgctcgc gctacggcaa gttcaagccg
     8761 tggctcgtga tcggcatcct ctccacgtcc gtcgttgtgt atctcgcgtt caacacgagc
     8821 ctgcagggct ggagcttcat cgcgtttttc ggcgtgatct atttcctcta ttcgatcacc
     8881 tacacgatgc acgacatctc ctactggggc atggtgtcgg cgctcagctc cgatccgcat
     8941 gtgcgcaatt cgctcacgtc ccggacgaac ctcttcgccg gcatcggcgg cgtgatcgcc
     9001 tcgatcctca tccccatgct caccaccggc agcggcacgc tcggcggcag caccgcaacg
     9061 gcctacggcc gcgtcgcgct cgtcatctgc atcctcgcgc cgctcttcct gtgcgcgacg
     9121 atcttcggcg tgcgtgagcg ccgggattac aatacgaagg aagtcccgcc cgtcagcttc
     9181 aaaaagatct ggaataccat caccggcaat ccgcagatcc tctggatctc gctcgttttc
     9241 ctgctgcagc agatcggaaa cgacgtcgtt atgggcggtc tcggctcaac gtacgtgtac
     9301 ttcgccttcg ggtatgaggg cgggctgtat tcgctcttca acaccgtcgg tctcgcggcg
     9361 acggcgcttc ttatgatctt ctatccgatg ctcgccaagc gcttccgccg caagcagatg
     9421 atggataagc tcatgctgct ctcggtcatc ggctacgccg ttatgatcgt ctccgggctg
     9481 gcgctctccg gcggtatggt gaagttctgg atcctcaccg tgggctacat ggtagcaaac
     9541 ctcggtcagt acgggttcta tctcattatg atgatctcgc tcatcaacac cgtggagtat
     9601 aacgaatacc tccgcggcga gcgcgacgag gcgatcatca cctccctgcg cccgttcttc
     9661 acgaagcttg cctccgccat cgtcgtggcg gtcacgtcgg cgacgtatct catcttcggc
     9721 gtcacgggct acaccaaccg gatctccgct cttgagcgcg cggcctcgct cggcgagatc
     9781 gccgaagccg aaaagctcgc gcagatcgat acgatcgtct cctccatcca gccgggtcag
     9841 tcccgcgggc tgctgctgtg cttgacggta ctgccgttcg tgctcatgta cgcaagctat
     9901 gtgctctata aaaaacacta cattctcgat gaagacaaat acgccgaggt gtgcgccgag
     9961 cttgaaaagc gccgggccgg ggcaaagtaa ggaggccgcc gatatggaca acgaaaatcg
    10021 ttcctgcatc gactgcggca gtgccgcctg ccgcggcaag ggcgggcagt tcccgccgtt
    10081 ctgccggacg gagcatctgg acgaggcgct gctcgagcgc tcactcgaaa ttttaaaggg
    10141 cgaggaaaac gccttttccg ttgccgcggc agtcaacgag cgcgacggct acggcacgaa
    10201 aagccgcatc gaggaaacga tgcacttcgc gcagcttctc ggcgtgaaga agatcggcat
    10261 cgccacctgc gcggggctga tccatgaggc aaacgcgctc gcgcgcgtcc tgcgcgcgca
    10321 ggggtatgag gtcacgggca tcgcgtgcaa atgcggcacg gtgaacaaga ccgagctcgg
    10381 catcccggag cgctgtctcg aggtcggaca gaacatctgc aaccccgtct tgcaggcgct
    10441 cgtgctcaac ggggaaaaga cggagctgaa catcgcgctc ggcctgtgcg tcgggcacga
    10501 cagcatgttt tataagtatt ccgaagcgcc ggtcacgacg ctttccgcca aggaccgcct
    10561 gctctgccac gcaagcctcg ggccgctgta tctgctcgac ggctactgga aacgcctcct
    10621 gaaacccgac ccctacctca ccgcgccgga gagcaaaacc gaagaaggaa aaaccgggga
    10681 cacgtcgaac tgacgtgttc tcgttttgta cgggaaaacc cggtgatctg cgatcgacag
    10741 gcgcatgctg caacattata gatacatata tatcctttgg tctgctttag acggagagtc
    10801 acggacaatg gctccgcaga acacgtttat gaaagcggaa acggtcgcgg cggcgcttat
    10861 cggaaaggcg gcgggagacg gataaaagcc gtcacctccg cgacgtactc caacaccggc
    10921 ccggagcggc gcaaggttcg gatcgagatg cgaaaaaagg cggaaaaatg atacagaacg
    10981 attttacgat ccatatccgg cgggcggaga gcggagacga ggcgtgtctc gcgcacgtcc
    11041 aggctgaaag caggaaggca gcgttcgcgg gcatcgtccc ggcggagctc cttgcccaat
    11101 gcacgagcat cgagcgcgcc gagaaaatgt acgcgcggct tctcgccgag cagcagggaa
    11161 acggctatat tctggagctg gacggcaagc cccactgcat cgcatggtgg gacgccgcgc
    11221 gcgaagaggg tatgccgggc gctgccgagc tgcggtgcat ccactcgctg ccggaaaact
    11281 ggcgccgcgg ctgcggcagc cggatgatgg agcgcgttct cgcggacgta aaggctgccg
    11341 gatacacaaa tctcctgctc tgggtgttca cggacaacgt gcgcgcgatc cggttttacg
    11401 aagcgcaggg tttcaccttc ggcggcagaa agcagcccgc gttcagcaca acggaggaaa
    11461 tgtacgtccg agcgctgtaa agccgtattg cggcacgcgc ccgtcgcggc gcagccgcga
    11521 tttttcggct cgcaggctgc cgaacgacag gtcgctgcgc tgccgaaatg caggagatgg
    11581 tattataaag aaaagcaggg ctaagcacgt tctgccgtgg acgagcgcag ctcgccggca
    11641 tcagcagcaa agacggcgcg tatagacctt aaagaaagga acgcgaatat ggaagcaaat
    11701 cttgaaccta agaacgagct cattgcatgc tgcgggctgg actgcgaaac ctgtgacgcc
    11761 aggatcgcaa cgatcacaaa taacgatgcc ctgcgtgaaa aaccgccgcc ctgtggacga
    11821 agctcaacgc cgtagcgatc acgccggaca tgatccactg caccggctgc cgcgcggagg
    11881 gcgcaaaaac gccgttttgc gacaagctct gccccgtaca taactgcgtg cgggagaagg
    11941 ggctggatac ctgcgcggac tgcgggcaga tggatggatg ctagacactg gggcgcatcg
    12001 ccgcaaacag cccgtttgtt ttggaaaacc tgagaaagct ccgctgtgag gagccgttgg
    12061 aacagaacga tgcctgacac aatgacggta aacgatacgg aatatactgt gctccggctt
    12121 ctcggaaagg gcaagggcgg ctattcctat ctcgtcacgg acggggagcg ggagtacgtt
    12181 ctcaagcaga tccaccacga gccgtgtgag tactatacct tcggcgacaa gctgcagtcc
    12241 gagctgcgcg actatgaaac gctgcgcgct ctcggcatcc ccctgccgcg gctgctggcc
    12301 gtggacaagg cgcaggagcg tctcttgaag gaatatatcg cggggccgac cgtcgcggag
    12361 ctgcgtgaga gcgggcggat ggacgggcgc tggacggagc aggtgcgcgc catgtgcgcc
    12421 gttttatacc ccgcggggct gaacatcgac tattacccca ccaacttcgt cccgcgcggc
    12481 gaaacgctct attatatcga ctacgaatgc aacgcctata tggagcaatg ggactttgag
    12541 cactggggcg cgcagtactg gacagagccg ccgtccgcgt cgaattgaac agcttgcccg
    12601 ccgggagacc ggcgggcaaa atttttttca aaaccctatt gacaatccgg ggatgtgctg
    12661 ctatattaac agtaacgata atcgttattg attacgaaac cccacggcga aggagtaccc
    12721 catggaaagc acccggcggc aaagcaaaaa gcgcgacgcg atccgcgaga cactgctcgc
    12781 gtcgtacgat cacccgagcg tggaggagat ctaccggcgg ctcaagccgg acatccccga
    12841 tctctcgctc ggcacggtgt accgcaatct cgcgctcttc cgcgaggagg gcaaggcgat
    12901 gagcgtcgcc acggttgcgg ggcaggagcg gttcgacggc cggacgcacc cccacgcgca
    12961 tttcatctgc gagaagtgcg gccgcgtgga cgatgtggac gcggtgctgc ccgaaacgct
    13021 tgtcaccggc gaaatgcccg gcgaggtgcg ctcctgggct ctgacatttt acggcatctg
    13081 ccgctgctgc gcagcggacg ataataaaaa caacaaagag aaaaattgat ttgatggagg
    13141 atcttattat gaaaaagtgg gtttgcccgg tttgcggtta tgttcatgaa ggcgatacgc
    13201 ccccggcgga gtgcccggtg tgccatgtcc ccggcagcaa gttcacgctg caggcggagg
    13261 agaagacctg ggcggcggag cacgtcgtcg gcgtcggcaa ggtgttcggc gcggacgttc
    13321 ccgccgaggt gcaggaagag atcatcgccg gtctgcgcgc caactttgag ggcg
//
LOCUS       DAWAAE010000045        13228 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2107_length_13228_cov_3.530555, whole genome shotgun sequence.
ACCESSION   DAWAAE010000045 DAWAAE010000000
VERSION     DAWAAE010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_13228_cov_3.530555"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5706..5715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2331 a         4029 c         4187 g         2671 t
ORIGIN      
        1 gctcggaggc gtcctcggcg gggatgaagt cgcccagatg ggagtcctcc tcctcgccga
       61 tcggcgtttc gagactgacc ggctcctgcg cgatcttgag aatgtcgcgc accttctcca
      121 cggggatgtt catctccgcg gcgatctcct cggcgctcgg gtcgtggccc agctcctgca
      181 gcagctggcg ggaaacgcgg atgaccttgt tgatcgtctc gaccatatgc accggaatgc
      241 ggatcgtgcg cgcctggttg ataagaccga gattgccttc ctgaatgaga tcgaggaaca
      301 gcataccgcg gccgacataa cgcttggcga tgctcacgac gagacggagg tttgcctccg
      361 ccatgcggcg cttggcctcc tcgtcgccct gcgacatgcg cacggcgagc tcgatctctt
      421 cctcctgcgt gagcaggctg accttgccga tctccttgag atacatgcgc acggggtcgt
      481 cggtcgagta ggaatccacc atgctgtcgg cttcggcgat ctcctcttcg gtgacctcat
      541 cgatctcctc aagctcgtca agcgcgggaa ggagctcgtc atcgtcgatg ggcggcagat
      601 cctcgccgac ggtctcgatg ttcagctctt cgagcgtatc gtaaaaccgg tccatctgct
      661 cctgcgagag attcagcccg tcgatgacgt ccgcgatctc cttgagcgtc agctttccgg
      721 cctttttgcc cttttcgatc aggttggaca ggcgctcctc gcgggatttc tgcgtgtcct
      781 ccggcttttc cttcttgccg cgcgcgccgt gggtgggctc aagctcgtca tcctcgggaa
      841 cgtccgcgat ctcttccggc tcgtcggtcg cgagaatggc gtcggccagc gccgtaagag
      901 cctctccctc gctgacgggc gcggattcgg gcagcttcgc ctccggcagc ttttcttccg
      961 gcatgttctt ttctttctgc tcgctcatcg tttatcctcc aaaacttttt gtctttctgt
     1021 atttttccgc cagcgcgcgg agatcgtcct cggcgtcctc cgtgcgtccg gtaataacgg
     1081 ctatgtaatc ggatatggcc ttgtccacgt gggaaagatc ctccggcgcg ccgagcaccg
     1141 aggtgaagtg gctcatctcg tcgccggtga actgtccggc cagcaccgcc atggagatcg
     1201 tttcgccggt ctgcacgcgg cgcagaagct cgcggtagag ccgcgcgagc acgggagagg
     1261 aaaagctctc cggcggcggc agggtcttgc ccttcgccgc gcccggatcg agatacagta
     1321 tccggatcag gccttcctcg gcgcgcgccg agcgcggatc gtcgtaccgg atggagcgct
     1381 gcgcgggctg cgccgtctgc gtagggcggg cggcgtcgcg cgcttcccgc tttttcgcgt
     1441 cggcaatgcg gcggcggcgc agccgttcgg cctcctgcaa caccgcctcg cgcgatacgc
     1501 ccgccttttc cgccacgcgc gcggcgtata cctcgcgctc gaccgcgccc ggcagcgacg
     1561 ccagaagcga ggccgcgctc ttgagatagt ccacgcgccc ctcgtcggtc gtgggcgggt
     1621 ttttcgcttc cacgtcgcgc agccggtatt cgatctgtcc ggcgctcgcg tcaatgagat
     1681 tgcggaaggc gtccgcgccg tttttcttga tgaactcgtc cgggtccttc gcgcccggca
     1741 cctgcaatac gcgcaccttg agatcgagct tctcgagaat gccgatgccg cgcgaggccg
     1801 ccttttttcc cgcctcgtcg ctgtcgtagc agagaacgac ctcgttcgtg tagcgcgaaa
     1861 taagccgcgc ctgctcgggc gtgagcgacg tgccgagcga ggcgaccgcg ccgtcaaacc
     1921 ccgcctgatg cagggagacg acgtcaatgt tgccttctac gagaatgata tacccgtttt
     1981 ttgacttttt agccagattc aaaccgaaaa gattatggct tttgctgaaa acgggcgttt
     2041 cggggctgtt taagtacttc ggctcgccgt cgccgagaat gcggccggaa aagccgatca
     2101 cgctgccgcg cacatcgatg accgggaaca tgaggcggtt gcggaaggcg tcgtacacgc
     2161 cgccggactt gccgtgcttc acgagcccgg cctcgaacat ctcctgatcg ctgtacccct
     2221 tcctgcgcat cgcgttcgcg agcgattccc acgaatcggg cgcgaagccg agaccgaagc
     2281 gcgtgaccat cgcgggggaa atggcgcggc ggcggacgta gtcctgtgcc ggggcgccct
     2341 gcggcgattt gagcatatca tggaaaaacc gcgccgcgtc ccggttgagc tccaacagcc
     2401 gcgcccggcg ggacgtatcc tcgctctgcg cttcctccgg cagaggcatg ttcgcgcgcc
     2461 gcgccagaaa cgccacggca tccgggaacg agagattttc gatctccatg atgaagttga
     2521 tgacgccgcc gcccttgccg cagccgaagc agtggtagat ctgcttatcg ggcgcaacgg
     2581 agaacgaggg cgttttctcg ctgtggaacg ggcacaggcc gaagaggttc gccccgctct
     2641 tttttgtcag ccggacgtac tgactcacca cgtccacaat gtcgttgcgc tcggtcagct
     2701 cgtcgagaaa ccgttcggga aatgccatga tacgtccgcc tcctattgca ataaattttg
     2761 gttggaagcc cggcatccgg ccgggcttct ttcgtttgct gtgcagcgct tatttcacgc
     2821 tccacgccat ggggatgaat atctcgccgt actgatagat cgcgtagtcg tccgtcatgc
     2881 cggagacata gtccgtcacg gcgcgctcga gcccgtcgcg ctccataatg ccgcggaaat
     2941 cgtccggcag cttctcggga taccggcagt aatgctccca gatgccgcgc aggatgttct
     3001 cgaccttggc ctcctcgccc ttggcgacgg ggttgtggta cacgcggtca taaagaaatt
     3061 cgtggaacac gtcccagact tcctgcatat cgtccgaaat gccgacattc cccgcgctgc
     3121 tcgtccggat gatgttatcg acgagcgagt tgatgcggcg gctgttgcgc gtgccgagcc
     3181 gctttacgat gatctccggc agctcggact ccgcgacgat gccggcgcgc atcgcgtcgt
     3241 ccagatcgtg attcagatac gccacatggt cgcacagccg cacgacgacg gcctcgcggc
     3301 tctccttggg atggcccttt gtgtgggcaa gaatgccgat gcgcgtttcc tggcagagat
     3361 tgagcccccg gccgtccttt tcgatgcagt ccaccacgcg cagcgactgc tcgtaatgcc
     3421 ggaagccggg gccgtaaatt tcgttgagcg cccgctcccc ggcgtggccg aacggcgtgt
     3481 ggccgagatc gtggccgaga gcgatggcct cgcacagatc ctcgttgagc cgcagcgccc
     3541 gcccgacggt gcgcgcaagg cgcgttacct ccagcgtgtg cgtgagccgg gtgcggtaat
     3601 ggtcgccctc cggctggaga aagacctgcg ttttgtgctt gagacgccgg aacgccttcg
     3661 agtgcgtgat gcggtcggca tcgcgctgaa agcatgtgcg cacggggtcc tcttcctccg
     3721 cgcggcggcg gccgtaggaa tcggcggcgc gcacaccgaa gtcacccacc agaatttttt
     3781 ccagctcctc gcgttcctgc cgcggacaag tcatgcgctc cgcctccttg aaagcaaaaa
     3841 agtaatgcag aagacctctg cattacttat atacgacatt gcccgcgatt ttccctgctt
     3901 ttcggagaaa aattttttaa attattcttc ctcccgctcc gggcggaaaa cgctctcgcc
     3961 gagacgcacc tcggcttctc cggcggtcgc gtcgcgcagg agcgtcacaa agctctcgct
     4021 ctgcgtctcg ggcgcggaaa aaacgatctc gacctccgcg ccgaagagtg tttcctcaac
     4081 gcgcgcgccg cgctcttcca aaaggcggcg cagccgctcg taccgggcgt aggagcacgc
     4141 gaagctcccg cgccgccacg ggcgcatgac ggcggtgcct gcctcgcgca gcgcggcgcg
     4201 ggccgcggcg gagtaggcgc tcacgagccc gcccgagcca agcagcgttc cgccgaaata
     4261 gcgcgtcgtg acgcagcaga cgtccgtcag ccccgcgccc tgcagcacgg cgagcgtcgg
     4321 ctggccggat gtgccgcccg gctcgccgtc gtccgaccag cggagaatgt tcccctcgcg
     4381 caggacgtag gcgaaaacgt tgtgcgtcgc gtcggcatac gtccggcgca tttcgtcgat
     4441 gaacgccttt gcctcgttct ccgatgtcac gggctttata tggccgataa accggcttcg
     4501 tttgtcctca tattccgcct cgccgtatcc ggcggcggta cggtacggcg tcattgcttc
     4561 agctcctccg tgatgcgtgc gagcagcgtg tccacgccct cgccggtgcg cgcggaaatg
     4621 cacacgccgt cgcgcgtgtg gggaagctcc gtaagcgccg ggatcgcgtc gcacttatta
     4681 taaatgcgca gcaccggcgt ggcggacgct cccagctcgc ggatgagcgc gtcggtgacc
     4741 ttcgcctgct cctcccagtc gggcgcggaa acatcgatga cgtgcagcag gagatcggcg
     4801 tactgcaatt cttcgagcgt ggccttgaac gcctcgatga gattcgtcgg cagcttgcgg
     4861 atgaagccga cggtgtcgga gagcaggatc tcccgctgaa gctccggcag ccagagacgg
     4921 cgcgtcgtcg tgtccagcgt atcgaaaagg cggtcgttcg ccgggatgtc cgagcgcgtc
     4981 agacggttga gcaccgtgga ctttccggcg ttcgtgtatc cgacgagcgc gaccatcggc
     5041 atggcgtttt tctcgcggcg gcggcgctgc gtcgagcgga cgcggcgcac ctgttcgaga
     5101 tcggcctgca gattctggat gatccggcgg atatgccgcc ggtcggtctc gagctgcgtt
     5161 tcgccggggc cgcgcgtgcc gatgggcgcg ctcgtaccgg cctggcgctt gagatgggtc
     5221 cacatgccgg tcagccgcgg cagcagatat tcatactgcg cgagctgcac ctgcagccgg
     5281 ccctcctttg tgcgggcgcg ctgggcgaaa atatcgagaa tgagtccgct ccggtccacg
     5341 acgcgcacgc cgagctcttc ctcgatcgcc cgcgcctgcg agggcgagag ctcgttgtcg
     5401 atgactacga gatcacactc gttgttcgtt acgatctcct ttatttcctg caatttgccc
     5461 gcgccgagaa agctgcgcgg gtcgggcgac tggcgggtct gcaaaaccat ggcgaccgtg
     5521 tccgcgcccg cggtgtccac gagcgcggca agctcctcca tcgaaatttc ggtggagcgc
     5581 tcctcggcgt ccatgctcga tgcggaaagc cccgcgagca ccgcccgttc tcttttctgc
     5641 gtcaagtcct gaatgggtaa accctcccgg tcaatcgtcc gtttcatcct cgggcagctt
     5701 ctccannnnn nnnnnacgcg aagcccgtcc tcgcccatgg cgagcacctt tacgcgaagg
     5761 ccgtccgcct ccacggtatc gcccaccttg gggaacgcgc cgaacttctc gatcgtccag
     5821 ccgccggcgg tggcgctcgc ggtgtcgagc gattcctcgg tcacgtccag aagctccgca
     5881 agatcgccga tggggagatc gccgtcgatc tcatacatgc cgtccggccg ctcaacgatc
     5941 tcgtcttcga cctcgtcggt ctcgtcccag atgtcgccga caagctcttc gagcacatcc
     6001 tcgagcgtca cgatgccgag cgttccgccg tattcgtccg tgacgacggc gagatgctgc
     6061 tggtttttgc gcagctgcgc gagcacgtcc ggcagcttca cggtcttata gatatacagc
     6121 ggcttcataa ggttgctgcg gatgtccgcg tcgggatcgt cgagcagcgc cttgtaaaag
     6181 cggttgagat acagtacgcc gatgacattg tcgatgctgt cctcatacac ggggatgcgc
     6241 gaatacggca gatcctcgcc cgagagcaaa agctcgcgcc agtcgtcgtc aatatcgagc
     6301 gcctcgacgt ccacgcgcgc ggtcatcacg tccatgacgg cgatctcgga aaagtcgagc
     6361 gcgctgcgca ggagctcgct gcggtcctcg tcgatgacgc cctcgtcctc gacggtctca
     6421 atgatggact gcagctcggc ggcggcttcc tcctcggtat tttcgccgct gtgctcgtcc
     6481 ttgagcggaa gcgtaataag acggatgagc agcgaaacga ggaagatgac cggcgagagc
     6541 acgatcataa gaaagcggac gaacggcgcg accgtcatcg aaaagcggtt cgcgtttttc
     6601 tttgcggcga tcttcggcgc ggattcgcag aacacgatga tgatcgccgt catgcagagc
     6661 gtaatgagga ccgacaccca gccgttatat ttttctccca gcagcgcaat gccgagcacc
     6721 gtcacgatcg agtccgagcc gatgttgcag aggttgttgc cgatgagaat ggcggagagc
     6781 gcgttttcgt atttgtcgat cactttgcag gcgatcccgg cggcgcggtt cccctcctcg
     6841 gcggcggacg caagacgcat ccggttggcc gacgagagcg ccatttccga tgccgagaaa
     6901 aacgccgaac cgcaaagcag cgcgagcatc aaaagcacat agatccacgt cgtcacgcct
     6961 cctcgccccc ttcctgcgtc tcttcgggca gaagctggat aagcagcttg cggatgcgcc
     7021 ggtggtcaat ggcctccaca gtcaccttca gccggtgcca ggtgaagctc tcgccctgcg
     7081 cggggatcat gtcgaactgc tcatacaccc actcgccgac gggcttgtgc tcccagtcgg
     7141 tatcctcggg atcgtcgtag ccgagctcgt cgaaaaggtc ctccatgtcc atgtcggcgc
     7201 tgacctcgta ggtatcgccg ccgagcgcgc ggaattcgac ctgcgcgacg tcgtcctcgt
     7261 cccagatctc gccgaccagt tcttcgatga tgtcctcgac ggtcacgatg cccatcgtgc
     7321 cgccatagtt gtccgtgacg acggcgagag agaccttctg ccggttgagc tcttcaagca
     7381 gctcgtcaat ggacgtgctc tggtgcacga agcacggcgc gtcgagcagg ctgcgaagct
     7441 ccgggtattt cctctcacgc aggtatacgc ggacgtattt gcggatctgc agcacgccga
     7501 tgatgttgtc gatgcttccc tcgtacaccg gcaggcggct gtggcgctgc tcgcggatga
     7561 acgagaggat cttctccggc ggcgtgttca catcgagcgc cgcaacgtct acgcgcggcg
     7621 tgaacacgcg ctcgactgtt acgtccgcaa actccagcgc cgagtggacg agctccgccc
     7681 ggtcctcggg cagctcgccc tcgtcggtca tcgtttcgat gatgtcgtag agctcatcct
     7741 cggtcacggt gatctccggc tcgcccttgg cgagattcga gaagaattcg ccgatgtgcg
     7801 aaagcgcctt cgccagcggc gcgaaaatgc gcataaaaaa gcacagggac ggcgccgtcg
     7861 caagggcgca ccgttcactg tagcgcttgg cgatgctctt ggggagcatc tctccggcaa
     7921 aaaacaccgc gatcgtgcac acgatcgtgc caagcgccac ccagctcgcg ccccaggtgc
     7981 gcgtaacgag caccgttgtg agcgtagcgg tggagatgtg gacgatattc gtgccgatga
     8041 ggatcgcgga aattgcgcgg tcaaaattgt cctgtacgta aagggctctc ttcgccttct
     8101 ggtcgccctg atcgagccgc gtcttgagtc ggatgcggct gacggacgtg gcggccgtct
     8161 ctgccacggc aaaatacgcc gccatgcaga gaagaaaaac gataggtatc caagacagcc
     8221 aactgtcgcc gtccatgtgg ggtcatcaca ctccattttt atatagttat gacagaatac
     8281 catattgtgc cccgcgcgtc aagcgcttac cggacactcc gccgcttctg tctgcggaag
     8341 ccacgcgccg ggcgcacgca ccgcgcgggc cggcgcgatg tgcccgctca tgcgcgatgt
     8401 ctcgcgcggg ttgaggatcc ggatctcccc ggtcgccttg atgatggctt cggcgcactt
     8461 gctgacgccc ttcgtccatt cctcataggg gcataaagag agccggcgcg gctcttttcg
     8521 ctgccgcgcc ggcgtaaatg cgctggtggt tcagtcttcg gtactcatac cggcctgcgc
     8581 cgcaacagcc ttgtcttcga tcttgtagct gcggttcatg ccgtttttga gagcagcgaa
     8641 gaacgcttcg gagtccttgc tgtcctgttt ggtgaagaga ctcacaacga ccatgcagat
     8701 cgcggcgccg agcatgccga tcgcgccgta ccaggtgccg ccgatgttcc acacaaagta
     8761 ggttgcgagc acgagcaccg tgccgacgat ggaggaaacg atggccgcct gcttcgtcgc
     8821 cttcttccag tagatgccga gcaccagcac cgggaagagc ggcatgacga tcgcgatgga
     8881 gaacatgatg agcgtgaaga tgagctccgg gggatcgagc gcgagcagca tcgagccgat
     8941 gccgagcacc gcgatgatga tgcggttggc gttcacgccc ttcttgccgc tgagatccat
     9001 gtggaacgtg ttgcgcagca ggtcgtccgc cacgatggcc gaggcgaccg agaggaacgc
     9061 gttggaggtc gaaaggccga cggcggaagc gccgatgaag aacgcgacca tgaggatctt
     9121 cgcgagcacc gggttgaagc tcacaacgaa gttcttgacg atgtagggga tgatgagctc
     9181 ggtctcggag gtcggcagcc cctgcacgag ggcaagaccg atgagaccga tgagcatggt
     9241 gcccgtccag acgatgccct gcaggaccgg cgtcatgtag gaaaggcggc gctgcgcctt
     9301 gagatcgtcg ccgaagtagc cggagcgcac gaacacgtgc ggcagcatga tcgtccagcc
     9361 gatggcgcag gagaacggat agccgaagcg ggaggcatac ggcacccagc cgtccggtcc
     9421 ggggtaggag aagaacgagg ggacgtcatc ccagagcttc gtgaccgcct tgctcagcga
     9481 gccgccgaag ccgacgatga gcgccgagat aacgatgacc cagagcagga gaatgaagag
     9541 gagaccctgc accgtgtcgg ctgtggcggc agacttgacg ccgccgacac acacgaacac
     9601 cgtcatgata aggccaacga cgatgaccgc gagacggtag ttgatcgcgc cgtcggtagc
     9661 gtactgcgcg gcgttggcga tggccacgag caccgaggcg atgtacggga acgaagcgca
     9721 caggaagatg agcgcaagga tgatgcgcag cgcgcgggat ttgaagcggg cgtcatagaa
     9781 gtccgcggga ctggagaacg tgccgcccat ctcgcggttg atgatccaca ccttgttcat
     9841 cgtgaagtgg gcgatgatcg ggaagagggc gatgtagctc atctcgctga tccagtagcc
     9901 gagaccgccg cggtagtacg cgccgggtcc ggcgaagaat acccacgtgc tcataagcga
     9961 ggcgatgtag gtcataacga gcaccggcca tttgaccgac gcggtcagca ggctcccgcc
    10021 cgcggctttt ttcttgtcct gaacggtgac gtaaacagcc acgcccagga ggaagcacat
    10081 atagagcgca aaggcgatcc agatagtagt cggactcagc ataacttact tatcctcctt
    10141 cttttcgttt ctcagcttgt agatgcgata gaccttcggg aggaagatgt aggtgatgac
    10201 ccagatgggc accaccgtga gcgcggcgtg gatgagcgtc gctgtcggct ggttgtaggg
    10261 ggggacgccg gggatgttgt aggcgatgta gaggatcgtg gtgataatga gccagatccc
    10321 ttcggtcttg gtgatcttca tgtttctctc cttttccttg ttactcgttt ttttgcggcg
    10381 atcgcgggaa aaagggtaca gaaactccac cctgccggta ttaggcctcc ggcagggctg
    10441 gttccatcgc tattaacatt attttatcct gccggagaac ggacgggagg cgatccacac
    10501 gccctccggc aggaagctgc tgaaatcagt ctttgacgag agcggcgtgc gccttcttga
    10561 ggacctcgtc cacggccgca gccttgtcgg cgggcagcgc gtagtcggct tcctcggcca
    10621 tgagggcgta atacttctct tcggccttgt cgagcagggt cttgctgccc tcgcgctccc
    10681 aacggacgac gccgtcgcgg ttgaacatgt cggggcggtg gggctcctca aagtggcgga
    10741 aggtgtgctc gctcataagg aagctgaacg caggatcctt gtcgatgcct tcgatgagct
    10801 catcccagtc gatggtctcg tcgttgacgt caagaccggc gcgcaggcgc tgggcaatgc
    10861 ccatgatctc gttatcgatg atgagctgca ccggggagat ggtcttcgcg gtctcgagct
    10921 ggcccatgcc gccgaggacg gaggcgttgg acattgccat cgcgtggatg aggctcgtgc
    10981 gctcgatgaa gttctgcgcg tcgaggcaga ggctgtcggt gccggtgccg taggaatggg
    11041 cgcgaatgtt gtagccgcgc tcgaagaggt ccatgcagat gagacgggcg aaggtgatct
    11101 cggtgttgga ctgcagcgtg taggtgctct gcatatccat ggagaacagc agggtcgtcg
    11161 cgatgatcgg caggccgggg cagagaagct cgagcatgac gatctgggca agaacatcgg
    11221 cgcaggcgac gaacgcggtg ccctgggcgg tcacgggggt gttcgcgccg gcggtgggca
    11281 gagcgcaggg ctgcacgggc acgccgtact tcgcgctgag gtacaggatc tcggcgtcca
    11341 tgtgcttgta ggtcagcgcc gggacgctgc aggcgatcat gctgacgatc gggcgctcgc
    11401 ggagcttgtc gtagccgccg acagccgccg cggccatgtc gatgaggaac tgtacgttat
    11461 ccgcctcgta gggctggatc cagatgtgct tgccggagag cttgagagcg tcggcgagaa
    11521 cgtaaacgtc gatcgcatcg ccggggtagt actcgccgga gacggagggc agcgtaacgt
    11581 agtccagatt ctccatcttg ttggtgagat gataccactc gtgctcttcc tcgggcttga
    11641 tgtagtgggt ctcgttcttg atgttgcggt agttgggcgc gcccgtgcag gtgcgggtgt
    11701 agaaccgtcc gtcgggggag gggaacttca ggtcgtgctt tccgtcgggg gagtagagcg
    11761 tgaactcggc gggaacggcg gcgatggcct tgtcgatgag ctccttcgtg aaacgcacgt
    11821 cgcgcgcttc catatcaacg acgcagccct tctcctcgag agccttgagc atctcctcgt
    11881 ggtcgaggtg gacgccgcgc tcgatgagga tctgctcgag gcgacccttc atgtattcga
    11941 gctctttctg ggaaagaacg tcgtacttaa tacgctgatg cataatcttt atcctcctta
    12001 acttttgtgc gctcaggcgc ccttggaagc ggcgtaggtc ttcgcccact gcgcagcctc
    12061 ggccgcgtcg gcggtgaagg catccgcgcc gatcttgtcc gcccacatct gcgttacggg
    12121 agcgccgccg accatgacct tgaccttatc gcgcacaccg gcgtcggtga gctgctggat
    12181 gatgtggccc tgctcgttca tggtggtggt cagcagagcg gagaggccga tgacgtcggg
    12241 gttgtacttg ttgatggcgt cgatgaactt cgcgccgtca acgttgctgc cgaggtcgat
    12301 gacttcgagg ttggcgctgc gcatcataag cgcaacgagg ttcttgccga tgtcgtgcag
    12361 gtcgccctta acggtgccga cgacggcggt gccgagcttg gtggactcgc cggcggcgag
    12421 cagcggctgg atgacttcca cgcccatgtt catgaccttt gcggccagca ggacgttggg
    12481 aacgaacact tcgtttctgg cgaacttttc accgatctcg ttgatgccgg ggagaaggcc
    12541 gccgtcgagg atctcctggg cgccgatgcc ctcgtcgaga gcctgctgga ccttggcttt
    12601 gatggtgcgg atctgaccgg taattaccat ctgggaaatt tcattgagag tggacatagc
    12661 gtgatttcct ccgttaaaaa aatcttcttt tttacaattc ttcgtccgga agaggatcga
    12721 tcccgcaggc tttcagggcc aaattcaccg tttccaccgc gtcagcggca tattccgcat
    12781 tgtgctcgcg ggcaaaccgg ccgttgaccg gcgcgccgcc gacaatgatg tgccactgat
    12841 cgctttcctc cgcctcgcgg agagcgcgga tcgttttctt catgccggtg accgtcgtgg
    12901 tgagcattgc cgagatcgcg atgatgtggg agttgttctc ccgcgccgcg gcgacaaatt
    12961 tctccggcgg gacgttcgtc ccaagatcga cgacatggaa tccggcgctc cggagcatga
    13021 tggaaacgag cgtctttccg atggtgtgga tgtccccgct gacggtgccg agcacaacgg
    13081 tctccgaaag cgagaggacg tccgggccca cgataagcgg cgagagaagg tcgatcccgt
    13141 cctgcacggc tcgtgccgac atcatgatct gcgggatgaa tttctcgcgg cgggacagct
    13201 gttggccgag cgtgtccatg cacggcag
//
LOCUS       DAWAAE010000046        13090 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2135_length_13090_cov_3.040813, whole genome shotgun sequence.
ACCESSION   DAWAAE010000046 DAWAAE010000000
VERSION     DAWAAE010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_13090_cov_3.040813"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11264..11273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2540 a         3958 c         4015 g         2567 t
ORIGIN      
        1 gtatgcgtcc agtcgagccg ggcaagacgc tcgtccttct cgtcaatgga aatttccaga
       61 tagtcaaagc cgagctcctt taccgcgcgc agacgctcct cccaggtaag ctccggcggg
      121 agcgcctttt cgtaaaggcc gagcagatgg tttccaagca tgttcgatca ttcctttccc
      181 cataccccgt cggacgagcg cggcgcggcg gtgcttttgc ggatgtgcag ctgtgtgctg
      241 acgtggatgc tgcaggtcgt gcggatgcgc ccgcgcatct gctggtgtat gatgctgaca
      301 gcccagcgcc cgatgtcggc gcagggcacg tccacggtcg tgagcgttgg ctcgctcatc
      361 gcggagaacg gcagcccgtc aaagccgatc atggagatat cctgcggcac acggatgccg
      421 cgctcctgca tggcgcgcat ggcgccgagc gcaatgctgt cgttgttggc aagaaaagcg
      481 gtcggcagcg cgataccgcg gtcgagcgct tcgccgaacg atttgcgcgc gccgtccatc
      541 gtggggaaca cgcggcagat gagatcgtcc gagtacgaaa gcccaggctg gtgcatcgcc
      601 gcttcaaagg cgtcgcgccg ggcgttgtcg ttgctcgaag gtatggaatt ataaagaaag
      661 ccgatcttcc ggtggccgag cgaacggaga tactccaccg cctcaaatac ggcgccggca
      721 ttgtccatcg tcacggtcga aaacggcaga cgttcgagcg cgttgtccac gataacgaac
      781 ggcttctgca gcccgtcaaa gtatgagaga tcctcgcgct cgatctccgt gccgagcagg
      841 atcacgccgc cggtggtggc ggtgtcgatc agctcggcgc ggtttatggc ggaaaagtcg
      901 ctgaaggcgt tcacaagaag attgtagccg tggcggcggc attcctctcc ggcggcgtcc
      961 atgatctgcg tgacgaagcc ggggttgccg ttgacaagat aggagtgctt gctgtatttg
     1021 agaaagcata gattgctgtg ggcgtggatg cgttggggcg cgtcctccga acggatctgg
     1081 tagccgtttt cctgcagaag ccgggtgacg agcgcccgct tctcttcgcc gatgccgggc
     1141 tttccgttga gaacgaggga gaccgccgtc agcgatacgc cggcagcgtc ggcgatttcg
     1201 cgcattttca tccgtagtgc ctccttatgg ttgtttagta aaacgatgct tatgtacatc
     1261 tttattctat atgatttcac taaaaatgga aagactcttt tcacagaaaa accatataaa
     1321 aatgcggcgg taaacttgga caatgttctg agtatgttat gtgcttcgga taaaatatcc
     1381 ataagagcgg gggatagtat gcggatgata gagcggaatg acgaaaaacg acgggggttt
     1441 ttgtttattg taccgcattg acatacgaga ggaaagaacg tatcattaag atacggtaag
     1501 cagcggagaa aaggggatgt ttactaaaat ttgtctctgc tgcgtaaaaa gccaataata
     1561 ggaggaaaga acatgaaaaa agcattggca ctcgtccttg ccctggtcat ggtgtttgct
     1621 ctgtgcgcct gcggtgccgc agccgagacc ccggcaactc cggagactcc ggcagctccg
     1681 gagacaccgg cggagacgga aaccccgacc gagggcgagc tcccctgggc gggacacacg
     1741 ctgtacgttg ccaactggca gggctacaac tccgacgaag attactgcga gaaggccttt
     1801 gaggacatga cgggcgcgca ggtcgagcac gtctacttca actcctacga cgagctgatg
     1861 accaccctca tgaccggcgg caacaagacg atcgacgcgg tcgttctctc caataactac
     1921 acccagtggt tccacgacga aggcctgctt gccaacgtcg atccggcgaa gatccccaac
     1981 tacgccgaag tggccgatgt ttacaaggac ctcagcccct acgctgtgga tgacgcgggc
     2041 aacctcttcg cgttcccttg gtgcaacggc acctcttctc tcgcctacaa ccccgacaag
     2101 gtcgattttg agctcgagca ctggtccgat cttctcaatc ctgcgctgaa gggtcatgtc
     2161 atggtcctcg acggcgacgg cgacgactgg gtcatcggct gcctgctggc gggcgaggat
     2221 tccgacgata tcgagaacgt tgatatcgag aaggtccgcg ccgcgctcaa ggagctcaag
     2281 ggacagctcc tcggcttctg ggcttccaac gacgagcaga ccatcccctg gctcgcgggc
     2341 gacttctggg cgggcgagat ctggtccggc ccctacagcc agctcatcaa cgatcccagc
     2401 accaacatca agctcgtcca ccccaccgag ggcacggtcg gctacgttga ctattgggcc
     2461 attgtggaag gcaccgatga gtacgatctc gcgtgcgagt ggatcaactg gatcgaaagc
     2521 gaagagcagc agaccgctat ggctaccggc atcagcgaca agtaccccgg cgagtactac
     2581 acctatacgc cggtcaacgc caaggccatc gataatctga cgccggagca gaagatcacc
     2641 ctcgagctcg acccgatgcc cacctgcatc aagctcctgc cttacatcgc tgatcccgaa
     2701 ctgaaggacg cctggaccga cctttatcag gaattcgtcg gctaaagcgc ccggcacata
     2761 acgcatccaa cgacagaatg ccgccggaac ccctttcggc ggcattcttt tttaaaagag
     2821 gggcggtaat tgaaaatgaa aaagaagaga accaaatttc gtccgggcgc gctgtggacg
     2881 acgccgagcg tcatcctcct gctgtgtctg acgatcctgc cgcttttgat gctgctcgtg
     2941 ttcagcttta tgaaccgcaa catctttgcc ggacagccct ggccgggctg gacgctgcgc
     3001 aacatcaccc gtatgttcgg ctccgcgacg ttctggcggc tgatgggaaa atcgctcctt
     3061 acggcgggcg aggtcacgct catctgcatc gttgcggcgt accccgcggc gtgggcgatc
     3121 gcaaagatcg tgaagccgca gcaccgcagc acgcttatga tggtcgtcat tctgccgttt
     3181 ttcacatcgc agctgctgct gatctacgcg atgatgaatc ttatccagac cggcggtctt
     3241 ctgctcacgg cgctcgacgc catcggcata cacggcgcga aaacgtggct ttatacgaac
     3301 aaggccacgg tactcatact gacgtatgag tatcttccgt atatgatact gtgtctctat
     3361 tcctcgctcg agggcatcaa cgacaacgtc atccaggcat cgctgatcct cggcgcgagc
     3421 aagatgaaaa cctttacgaa cattgtattt cccatgagcg tgccggggct gctctccggc
     3481 atactgctcg tgtttgttcc ggtggccggc agctttgtcg agccgggact tgtcggcgga
     3541 gcgggcggct ccatggtcgg cagcatgatc gatacgcagt actccggttc tctgaacatg
     3601 ggctacggcg cggcactttc ctgcgtgctg ctcattattc tgagcatcat catggccgtt
     3661 acccgtattg ccgccaaccg cgcgcagaaa gcgatcggag gtgatatcgc atgacgagca
     3721 aggcctttcg ccgcacgatg atggcatgcc tgtgcatcgt gctcgcgttc atgtatatcc
     3781 cgatcatcgc catcatcggc atgtcgttca acaaaacgcc gtacggcatg ttcccgtatg
     3841 tatttacgac ccagtggtac aaaacgctct tcacgacgag caaccttctt ccggcctgta
     3901 tcctcagtct gaagttttcc ttccttgtca cgctggccgc tgtcgtgatc ggcacgctct
     3961 gctcctacgg cctgcagtac tacgccccca agtgggcggc gcggttcaac ttcgtgctcc
     4021 agctgccgat catcatcccg tggctcgtaa cgtcgatctc cctgctgctg atgttctcat
     4081 tcctcggctc ggggcgtggc atgggcacca tgttcctcgg caacctgatc gttgtgctgc
     4141 cgtacgttgt gctgctcgtc aacggccggt ttcaggatat ggaccgctcg ccggaggcgg
     4201 cggcccggct tctcggggca agcccgctgc gcgtgttttt tgatatcacg cttcctgcca
     4261 tcacgccggg cattctggcc ggcggcatta tggcaatgat catgtgtttc aacaacttcg
     4321 tgctgcagta ttacctcatc cctgtcggca ggaatacgct gccgacgctc gtgttcaccc
     4381 gcatccgcag cgagtatcag gccgatctga acgcactttc ggcgatcatc gttctgatcg
     4441 cggttatcgt tgtgatcgcg ttctcgcggc tgggcttttc cgcgggcagc ctgttcggca
     4501 tgtccggcgg cgcgaagaag aaaggagaat aaccgatggc ggatctttcg gtaaaaggat
     4561 tgaaaaagag cttcggcgac catgtcgttc tcaagggcgt cagcttcgat gtaaaaagcg
     4621 gggactttct ctcgctgctc ggcccctccg gctgcggcaa gacaacgatc ctccgcatca
     4681 tctgcggtct ggagaccgcg gacgagggcg aggtgctcgt agacggcgcg gacgtgacga
     4741 aggtacgccc ggaaaagcgc aacatcggtc ttgtgttcca gaactacgcg ctgttcccct
     4801 ccatgaacgt ggcgaaaaac gtgggctacg gcctcaagat gcacaaggtc ccccagccgg
     4861 agatcgacga gcgcgtcaac gaggcgctcg agcttgtgaa gctcggcggc tataactccc
     4921 gccgcgtgac gcagctctcc ggcggcgagc agcagcgcgt ggcactggcc cgcgcgcttg
     4981 tcaccaagcc gaacattctc ctgctggacg agccgctctc cgcgctcgac cgcaaggtgc
     5041 gcgccgagat gcagtatgag atccgcaatc tccagcggca gatcggcacg acgaccgtgt
     5101 tcgttacgca cgatcaggag gaagcgctca cgatgtccga ccagatcatc ctcctccacg
     5161 acggccggat cgagcagcag ggcgacccct tcacgatcta cagccagccg gcgtccgtgt
     5221 tcgcgtccga ctttctcggc aaggcgaacc tcctctccgg cgttctcgcg tgtgaggacg
     5281 gtgtgtggtg cgtatgcagc gaaaacgtcc gcattccggt caaccacgtc ggcggcaggg
     5341 agggcgacac cgtcaagacg gcggtgcgcg gcgagtattt cgagttctgc acgccggaga
     5401 ccgagggcgc gaacccgttc catctcgaaa agaagatctt caccggtctt tcgtggaagc
     5461 tcatcggcac gctgggtacg cagccgctcg atatttccgc gctcggcacc cacgccggaa
     5521 cgctctcgga gggcgacgat ctcttcgtcc gcatccgccc ggagaacgtg gtctactaca
     5581 acaataactg attttcccct tacgaaagga gtttctgcat ggcaaaccag tacgaagccg
     5641 cccgcgacat atacgccgcc tggggcgtgg acaccgaaga agcgctgcgg aagatggaca
     5701 ccatccccgt ttccatcaac tgctggcagc tggacgatct caccggcttt gaggattttg
     5761 atgccgcact gaccggcggc atcgccgcca cgggcaacgc ccccggcaag ccgagaagcg
     5821 tggaggaata cttcgtctct ctcgacaaaa tgctctcgct cgtcccgggt gcgaagcatc
     5881 tggcgctgca cgcggtgtat ccgctcacaa acggcgtgaa ggtgccgcgc aacgagatcc
     5941 gccccgagca tttcgccggg tgggtggact atgcccgcga aaagggcatc ggtcttgatt
     6001 ttaaccccac atacttctcc cacccgatgc tgcgcgataa ctggacgctc gcaagccccg
     6061 agaaggaagt gcgcgatttc tgggtgcagc acggcatcgc ctgccggaag atcggcgagt
     6121 atttcggccg cgagctcggc gaggtgtgca tcacgaacca ctggatcccg gacggctcga
     6181 aggacacccg cgtcgatacg ctcggcccgc gcgagcgtct cgtcgaatcg ctcgaccgca
     6241 tttttgccga gccgatcgac cggaagtata accgcgactc gatcgagggc aaggtgttcg
     6301 gcctcggcct cgagagctat accgccggtt cgcacgagtt ttacaccaac tatgccctta
     6361 cgaccggcaa ggccatggtg tgcatggata ccggccatta ccacccgacc gagagcgttg
     6421 cggcgaagct ctcgtcctac tttgtgttcg gccaggagat catgacgcat ctctcccgcg
     6481 ccgtgcgctg ggacagcgac catgtcgcca tcgtgtcgga ggataccgtc gccatcatgc
     6541 aggagatcaa gcgctgcaat gcgtttgaca aggtgcatct cggtctcgac ttctttgatg
     6601 caagcatcga ccgcgtgagc gcgtcagtta tcggcgcgcg cagcgtgaaa aaggcgctga
     6661 tgatcgccct gctcgaaccg acggacaagc tcatggaggc cgagcgcgaa ggaaacttca
     6721 cgcgccgtct cgcgctgctc gaagagttta aaatgctccc cgctggcatc gtctttgacg
     6781 agtactgccg ccggcacgac atgcccggcg cggattgggc ggcgaagctg tgatccgccg
     6841 ctttatcatc gacacggaca ccggctcgga cgacgcctgg gccatcgtcg aggcgctctg
     6901 cgccgaggac atcgcccgtg tcgaggcgat cacgaccgtg tgcggaaact ttccgctcga
     6961 tctctgcacc aagaacgcgc ttctggcgga ggacgcggcg ggaggctatc tcccgccggt
     7021 ctaccgcggt gtggagcacc cgctgctctc ccaaaagagc tttgcggcgt atcatgtcca
     7081 cggcgaggac ggtctcggcg gactcaacct gccagcaccg gcgcgcgagg cggaaaagaa
     7141 gcacgcggtg ccggctatca tcgaccttgt gcgcgaaaat cccggcgaga tcgagatcgt
     7201 taccattggc ccgctgacga acatcgctgt cgcgctcacg cttgcgcccg atctcgtgcg
     7261 gaagatcaaa aagctctgga tcctcggcgg cagcgccggg atccgcggca atctcaccga
     7321 gtgcgccgag tataacgtcg gctccgatcc cgaggcggcg gagatcgttc tgcagtccgg
     7381 cgctccggcg gtgtggatca cgctcgatac gacccgcggc agcaccgaga tcactccgga
     7441 cgaggtggaa cgtctgctcg cgtcgccgtc tccggcgggg cggttctgcg cgctcgccac
     7501 gcggcggctg cgtgaatttt ataacgagat ccgcggcggc agcgagacct acggcgttgt
     7561 ggacagtctc gccgtcacct cggcgctgta cccggagatc atgacgaagc tcgttgccgc
     7621 taagtgccgc atagagctgc acgacgcgga aaagcgcgga tatttccatt tcgatacggc
     7681 cgcgccggag gcggagcaca acgccgtgct ctgcaccgcg gtggataccg cgctcttcaa
     7741 acagcgcatg tatgcccttc tcggcgcgaa ataacaaaaa tcatgccccg tttcggtttt
     7801 cggaaacggg gcattctggg aaaggagaag atccatgaac gattttatgt attataatcc
     7861 cgtgcgcgtc cactttggcg cggacgccat gagccatctg ccggaggagc tgggcaagtt
     7921 cggcgcaaac gtcctgctca cctacggcgg cggcagcatc aagcgcaccg gcctctatga
     7981 ccgcgttctt gatgtgctgc gcgcggcggg gaagagcgtc tatgagctct ccggcatcat
     8041 gccgaacccg cgcaccgaaa aggtgtacga gggcatcgag ctttgccgga agcataagat
     8101 cgacctcatt cttgccgtcg gcggcggcag cacgatcgac tgctccaagg ccatcgccgt
     8161 gggagcaccg gcggagggcg acttctggaa aaggttttat gtaaactggg agagcgccga
     8221 aaccggtatc cctctcggcg tcattctcac cattccggcc acgggcagcg agctggacaa
     8281 gtccgctgtt atcacaaatt tcgccactgg cgagaaaaac tgctatgaca gtgagtatga
     8341 gttcccgcgc tttgccattc ttgacccgac gctcacatac agcctgccga aaaaccagat
     8401 ggtgaacggc attgtggaca ccatgtcgca cctgatggag ctttatatct ccccgccgga
     8461 cgacgattcc ctcacggaca atctcgccga ggccgccatg cgcacggaaa tcgccgcggc
     8521 gcggcgagcc atcgtggaac ccacggacta tgaggcgcgg gcgaacctta tgtggacgag
     8581 cagctttgcg ctctgcggca tgctcaataa cgggaaaaag accgactggg agagccactg
     8641 catcgagcat ccgctctccg cgctcttcga cgtggcgcac ggcgcgggtc tcgctgtcgt
     8701 acacccggcg tatctcgagt atatctgcga gagcgccgcg ccgcgccttg cccgcttcgc
     8761 ccggaacgtc tggggcatcg atcctgccgg gaagagtgag agcgagcagg cgaaagaggg
     8821 tatcgccgca ctgaagaaat tcttccatga agagctcgac gccccggcga cgctgcgcgc
     8881 tctcggcgtg ccggagagct gctttgcgcg cgtcgtggag ctcaccgatc tcagctgcta
     8941 cagctaccgg cagctcaccg cggacgatgt gatcgagatc ctgcgccgct gctattgact
     9001 gctatggaaa gagaaacgct cgaaaagctc tatgcagggc tcatcggcat ggacgccggt
     9061 atgcgtctcg gcgcgccggt ggaaaacccg ttctggacgt atgaacggct gcaaagctat
     9121 tacggcgaca tccgcggcta tctgcgcgag cagcggtatt ataccgcgga tgacgatgtg
     9181 aacggtccgc tcatctttgt gcgcgcgctt gcggacaacg ccatgcccga aacgcttgcg
     9241 ccggaaatgg taggggagac gtggctcaac tacacccgcc gcggcatggg catgttctgg
     9301 tggggcggcg aggatgtttc caccgagcac cgcgcgtata tgaacctccg ccgcggcgtg
     9361 aaggcgccgc gctccggcag catcgaggaa aacggaaaga ccgccgccga gcagatcggc
     9421 ggacagatct ttgtggacac ctgggggctc atctgccccg gcgacccggc gctcgccgcg
     9481 gatctcgccg ccgcagccgc gagcgtatcg cacggcggca gcggcgtgga cggcgcgcgg
     9541 tttatggccg cgtgcgtcgc cgcggcgtat acggcgtcct cgctggacga ggtgctggat
     9601 gccggtttgc gcttcctgcc ggagaagtgc gactaccgcc gcgtggtcga gacggtgcgc
     9661 gcctaccacc gcgcacaccc ggcggaagcg gatttccgcg cctgccgcga gatgattgcg
     9721 cgcgagttca gcgacgaaga atatcccggc ggataccata tcgtgccgaa cgccgggatc
     9781 tgcattctcg ccatgcttta cggtaagggc gagcttggcc gcagcatcga gatctccgtc
     9841 atgtgcggat acgataccga ctgcaacgcg agcaacatcg gcacgatcct cggtgtgctc
     9901 ggcggtctcg gcggcgtacc ggagcgctat cgccgtccga tcaacgatct tgtcacgctc
     9961 tcgagcgtgt ccggctatct caatctcgtc gatctttccg acaaggcaaa ggagctcggc
    10021 gcgctttcct gccggatgta tggcggcgcg ctgccggagg atatcgtcag cccaaagccg
    10081 ggcgagctgc gtatggactt tccgttcccc ggctcgacgc acgggctcga gctgtcggat
    10141 aaggcggagc acacgctgcg catcgttccg ggaaaggcgc actccggcgc atactgcgcc
    10201 gagcttatga ccgacggcaa gcgcgccgga ccggtcgatc tctcgtttaa ggcgatgctc
    10261 acgcgcccgg atctccacga ggagcgctat gatcccgtgt ttgccgcgaa ggtcaacccc
    10321 ggtcagcgcg tcggcgtctg gatgaaaagc gagcagaccg cccctgccgc ggtgacggtg
    10381 acgccgttcg tccgccgcga catgactggc gagagagtga acatgtccgc cgcggtgctt
    10441 tcggaagggg agtggacgga gatcgtgttc accgtgccgg agctcggcgg tgacgctgtg
    10501 cacgatgtcg gctggacggt cgagacgaag cccgacgccg acccctgggt catgggccgg
    10561 gtgtatgtgg acgagatcac cgttaccggc gcaatggact atgcggtaaa tttttcgctc
    10621 cagcgcgagg agttttcgca gctcacgccg ttctcgttca acgactgcgc cgggaagcgc
    10681 gagggggacg ccatgcgtct cacggcggag gcgctgccgt atatgaccgg cgcgcaggcg
    10741 ttcaccggga attactatct gcgcgatacg gcggtgagcg cgtctgtgac ggttgaaagc
    10801 ggcgagagcg cccttgtgct tctgcgcggg cagggcacac gccggtatta tgcgctcggc
    10861 ttttccgcgc cggacgagtg cgccgtgctc cggtacgagg acggtcgggt gacgaagctc
    10921 gccgccgcgc cgtttgtctg gcagccggga cgggaatacg ctctccgggc ggaggcaaag
    10981 ggcgaggtgc tcacgctttt cgtgaacgga acgccggtgc tcgaagcgag cgacagccgg
    11041 tatgcctacg gcatgcccgg cctcggtctc accgctgcgg gcgagacact ctggcgggcg
    11101 ctgcatattt ccggagaatg ctgatgctca cataaaaaaa ttccgtatgt cgtcggacat
    11161 acggaatttt tttatttatc cggcgtgtcg gtacgctgcc ggaagaccca gcgcttctgg
    11221 atctggtagc ttgccaggaa gagcacgccg tccaccggga tctnnnnnnn nnnttgatga
    11281 ccgtttcgag caggtttgtt gcatttgtga gatcctgcaa taccgaaacg agcaccgccg
    11341 acaggctcgc ctgcacaacg gcaagcacgg cataccgcca gatcgactgc cccgccgcgc
    11401 tcttatcctg aaacacgagc agccggttga ggaaaaaatt cgtcacggcg gaaacgatgc
    11461 gcgcgccgta ggtcgcgcag agctcgcgcg tgccgtccgg cagggagacg agaagcacgg
    11521 agttgagcag cgtgaacagt ccataatcca gcagaaagca gatggcgctg ctgccggaaa
    11581 agcggaggaa cgatttcaaa gcggctttac tccttcttgt ccccatggaa gatgtggcgg
    11641 aagatcagct tatagatccg ccaggaatcc ttcacggtgt tgaagtggct ggtctggttc
    11701 tcgtcgatgt aaacggtatc gatcacgacc tcgccgacgc cgagccccgc gcccttgagc
    11761 gagaggagca tctgcgtttc atattcgtac cgctcgccgc cgatggagca catcagcggc
    11821 aggtgcttgc gggcaatggc gcgcagtccg gtctgcgtgt cggtcacgcc gacgccgcag
    11881 gcgagcttca taaagctgcg cgtgatcttg ttgccgagct tgctgcgcag cgggacctgc
    11941 tccagcgaga aatcccgcac gccgagccag agcttgtccg gctcgcggca catcgcctcg
    12001 gcaacggcca tggcgtcctt ggggcggtgc tgattgtcgc cgtccacgat aacgacgccg
    12061 tcgatatccg gccggttttc cacgcaccag gcaaacgccg ttttcatcgc gcggcccttg
    12121 cctttattca cctcgtgccg cagcaccgtg acgccggggc gggaggcggc ctccgcgaag
    12181 ggctggagat gcccttcgtg gctgccgtcg ttgacgacga cgatatcgtc aaagccttcg
    12241 gccagcaggc cgtccacgac ctgaatgagc ttttcgtccg gctcgtagga gggaacgaca
    12301 gcagtaactt tcatttcgct tttaccctgc ctttatcaga gagtttcgta gttgctgatg
    12361 acaaaaccgg cgtcggtctg caggaaataa atgcgcatgg tggcatcggc atagtcgatg
    12421 gcctgcccgt tgtgcgtgca gttggcgtcg taggtcacgt ccacgctgta gcagttgtcc
    12481 gcccagatca ctacgcccga ggcggtggtg ttggaaacgt cgatgttgcc gtaggcagtg
    12541 ttccagacaa cgccgttgta ggtgtcctgc agatcggtgt aggcctgcgt gccgggcgtg
    12601 agataggcga gcgcggcgta caggttgccc catgtgccgt tgtagccgtt catatagtag
    12661 gtgagatacg ccttcacgaa gctcaccgcg cgcgtggtgt acttttcggc atccgccccg
    12721 tcaagacaga gcatgaaatc gccctcggcg gtctttgtaa cgctgcatcc cgcgggcggc
    12781 tcggcggtca gctccggctc gatcaggaga ccgtccaccg tgtacgtcgt ccacgaaacg
    12841 gggttgatga gcttgtcctt gagcggctca taggagaagt tgttctgcgg ttctccggcg
    12901 tactcgctgc cgagctcggt gccgttgcag aacaccttgc tgccgacggc gacctcgacc
    12961 gaggcggact tcgttccctc aagctcaagc tccacgtcgg atacggccca gttcacgtcg
    13021 ctgccggaga gcgttacgcg ggcgagcgtt tcgtcgccgt ccttgagaac gtaggcgggc
    13081 gcttcatcgg
//
LOCUS       DAWAAE010000047        13009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2153_length_13009_cov_3.417477, whole genome shotgun sequence.
ACCESSION   DAWAAE010000047 DAWAAE010000000
VERSION     DAWAAE010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_13009_cov_3.417477"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7029..7038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2755 a         3751 c         3729 g         2764 t
ORIGIN      
        1 atgacggtgc tgcgcaccct gcggcaggca gaccgggaga cgaaggtgct gatcctctcc
       61 gcccgcgggg aggtggagga taaggtcgag gggctggacg cgggggccaa cgactatctg
      121 gcaaagccct ttcatctcgc ggagctggag gcgcgcatcc gcagtcttac gctgcggcag
      181 tttacgcagc gggatgtgat attgacctgc ggggcgctga cctttgatac gcgctcgcgc
      241 accgccgccg tcaacgggca gaccttgacg ctcacccgca aggagacggg gattttggaa
      301 tatctgatgg tgcatcaggg ccgcccggtc agtcaggagg agctgatgga tcacgtctgg
      361 gacaacagcg tggacaattt cagcaactcc atccgggtgc atatctccgc tctgcgcaaa
      421 aagctgcgcg ccgcgctggg ctgcgacccc atccgcaacc gcatcggcga ggggtatctg
      481 atgggagggg aggacgcatg aagcggcttt ctttgcagtg gcgcatcacg ctgctgaccg
      541 tgctgctcat cggcgcggcc tgcgtttcca tgaagctgct gctctgcgcc tccgggctgc
      601 actatatgga ctccatcggc agcttcattc aggattacgg cagcgccgcc gtggaaggcg
      661 atccggcgtt tttcgatccg gagcttgccg gaatgaacga ggatgtgacc attgtcatac
      721 acggcgcgaa gacgtccttc tgcacaacga actggtatat cacggcggcg gtgacggtgc
      781 tgagcggcgt tttggcgtat ttcgtcagcg gccgcgcgct caagcctctg cgcagctttg
      841 cctcgcaggt ggagaaggtg cagctgaaca atctcgcgga tatgaagatc gacgaggacg
      901 tgctaccgga gttccggcag ttcagccgct ccttcaacca gatgctggag cggctgaaca
      961 atgcctttgc cgcgcagcgg cagtttaccg gaaacgccgc ccacgagctg cgcacgccga
     1021 agaaaggcat cgcgctcgaa cgggagggcg acggcgttat gatcgggagc gacgcgctga
     1081 tctaccgaat gcttttcaat ctgacggaaa acgccgtcaa gtacaaccgc cccgatgggt
     1141 cggtgcgcat ttccgtggca caggaggaca aaatgctgca catccgcgtg gcggataccg
     1201 gcagcggcat cccggaggaa ttccggcgga gcatcttcca gcccttcttc cgcgtagaca
     1261 aatcccgcag ccgtgaatac ggcggcgtgg gtctcggact ttcgctggtg tgggaaattg
     1321 ccggtctgca cggcggctcc gttcgggtgg aggaaagctc ggagcgcggc accgtctttg
     1381 ccgtggagct tccggcacaa taaaaaagcg caaaccggcg gcgaggagag ttcgttctcc
     1441 cgccgccggt tttctttttt gccgcggttt acggcgtttt tgtcttttct tcggtctttt
     1501 ttctgtttat gagtctgtcg cagcaaaccg tcatgccggg cagaaccaga aggatcagca
     1561 gcatggcgac aagcgcgcca atggacagcg tggtgctcac atcggagatc atcggcgagg
     1621 atacaaggat cccgagcgcg gcaagcacca gcacaaggat ggagccggag gtcatgatgg
     1681 tgtggatgga gccttcgtac gccgcgcgca gcgcgtcggt cacgtccatg gtttttcgac
     1741 tgtccatata gaaattacag aacacaatgc cgtagtcgat cgttgctccc ataagaatgc
     1801 tctgtacgat cagcagcgca agataataga tcgagccgct gtaggctccg atgaccgtta
     1861 cggtgataaa tacgccgcac tgcacgagaa gcgtgaggat cagcggaaga atcggatggc
     1921 ggaacgcgat gagcacaaca agaaatacgg agatcgcggt gatcagggag atcatccggt
     1981 attcgctgtc aaaggcgcgg tccatctcac tgaccataac ggagttgccc accagatagt
     2041 attcactgag attttcccgg cagagcgtat cgatacgggt tacatagtcc gacgtttccg
     2101 gagactcgtc cgggtaatcg gaggtgacga ccattcttgc gtaggctttg cctctcatct
     2161 gcgcaacggc gtcctccagc tccgccttgc cgtcgaggat ctcctttttt gtttctccgt
     2221 cgaaatactc tgcaaagcgc ccgtcgttca tcattttgtc gcagagcaga tcaaagagct
     2281 ccacaatcgt cattttttcc gtgggatcgg cgtcattcac gcctccgtaa aggagataaa
     2341 ggatctccgt ctcgttttcg gtcacctcgt ccgtcagaga ggaaaggagc gcggtcattt
     2401 cgccggcggt gtaagctttt tccgaaacga cggcctcaat cagcgtatga ccctgccgaa
     2461 gctcctctgc ggttttttcg tcgatctgct ttgcaagctc tgcgtcgccg agcacgtccg
     2521 ttgcggcaaa ggctacaaac gagcgcggag agatcgtcca cgagtccgta ccgccgtttt
     2581 cccccatacg gaggatatac agcttttccg tctgcgccgg atccatgccc aggaacgagg
     2641 caagtcttgc ggaggtgaac gccgttccgt ttatcgcggc gttcagaatc gcctgcaaaa
     2701 actgaatacg gccgagcgtg gaggcgtcca tcattccgct cacaagagga tcggacagag
     2761 caaagtcggt aaacgccttc agcgacattg ttttttggct gatatccctg ccgaagtaca
     2821 ggcggaatgc catcgtgatc gtatccgtgt ccgtgtcgaa cagctgtgcc agcgtcccgg
     2881 cgtcaagcgg tgtgccggaa accgcgagct gcgcgatgga atccagccgg cgaagctgta
     2941 ccgcctgctc ctcggaaaat gctccggcgt aggccgtgtt cgtcagcacg gagttgacga
     3001 gaaaagcgga gaaatcggca agagccaccg ttttctgccc tgccagcaca agcgcgaata
     3061 tctggcagac ctgcgcggta tccatcccga acacggaggc aagggaagaa gcgtccagcg
     3121 cctgcccgga aaccgcgagc tgtacgagct gattcatctg ctgcaagccg gcgttgtcgg
     3181 gcgcctgtgt cagcgcggcg gaaagaaagt ccggaagcgt cattgcctcc gtgccgctca
     3241 tcagataaag ccccgcgaca ttctccgtgg tcatccccgt cacgcccgcc atggtttccg
     3301 cgtccaacgc ctgtccggaa acggcaagat tcacaagctg ctccgtttgg gcgagctgcg
     3361 cctgctgctc ggcgggggcg ttggccttca gcagcgggag aaagtcggta agcgtcatcg
     3421 tcatggcggc cgcctccttc tggaacgcgg gatcggcgga aaagtagagg ctgtacagct
     3481 tggaaacgct ttccgcgtcc atccccagcg tctgcgccat ctggtcgggg ggcagcccct
     3541 gcccggacac ggcaagctgc accagcgcgt ccatcgtctg cgcctgcgcc ttggcggcat
     3601 cgtcaaaata tgagctgaac agcgcatcct gcagcaggct gccgcacaaa aacgaactga
     3661 attcgggcag actcatagtt tgcgcgctga cgtcgcccgc gttatcgaga ataaacacgg
     3721 tattcaccgt gctctcgtcc gccccgatca gcgcggccag atcggccgcg ctgcgcgccg
     3781 ccagaacggt attggtattc gtataggttt gcagctgccg gagggaagca attgccgagg
     3841 cgctcaccat cgagccgtag gtttcgtttg ccgccacgga attgagcaca taatccacaa
     3901 agaccggcag cgtcattgtg ccgctgtccg caacgccgtt ttggatcgta tagtagagat
     3961 agagcttctc gacacgctcc cggtctatgc cgaagagctc cgccatttcg tcaacggaaa
     4021 tcgcggtggt caggcgcgtg cggtcggcaa gcgttcggag ctgctccgcg ttgtcccgca
     4081 gcgcggcgtc aaggtgtttg ccgatggttt tgtccggaag aacggtgtcg gttataaaat
     4141 taataaagtc agacgccgtt atcacgggca ttttcccgtc ctccgcgatg aaatagagca
     4201 tacggaggag gttatcgccg atgggctccg catcgtcgct caattcggcg acggccttgc
     4261 ccatgtcctc ggcggtcatc tccttgccga gcgtgttggc atagccgaga atatcggaaa
     4321 cccgctcgtc cttttccagt tcggagatga cgctgtcgat tttcgcttcg tcctcgttgc
     4381 ggtaaaccac cgcaacggtg ttcttcttcg ggaacacatt cgccagcggg tcgtcgtttt
     4441 cctccgtgaa gacgatctgc gtatagccct gcaatataaa ggaagcgata aaaagcgcgg
     4501 caaaaacggc gggcatggcg tatctcgtct tatagctgag ccttgagaag cgcgccatcg
     4561 gaacatgcgg cgattttttt ctcgtctttt ccagcagcgg gtccattgca aggatcatcg
     4621 cggggaggac ggtgaaggcg cagagcatgc tgatgaatac gcccttggca agcacgatgc
     4681 cgagctccgg cccgagcttg aagctcaaaa acacgagcgc cagcaatccc acgaccgtgg
     4741 taagcgagct ggaggcgatg gaggaaaccg aaccggcgag cgccgttttc atggcagaaa
     4801 ccttgtcgct gtgcgtgacc ttttcctgcc ggtagcgatt catcaaaatg atggaatagt
     4861 ccatggagag cacgagctgc aaaatgggcc cgaccgttgc ggtcatctca tcgacgtacg
     4921 ggaacaggat gttcgttccc atgttgagca caacggcgac gccgatggtg acgagaaaca
     4981 gcacggggtc cagccatgaa tcggacatga ccagcaaaat gataacggca agcgtcagcg
     5041 caagaacgac gacccagagc ggaagctccg ttgccgggac atcgttattc tcatacgcca
     5101 tcgtatagcc ggggaagccc tcttcgatag cggcttcgat cgcgtgctcc tcgtcggtgt
     5161 cgtaatcgta ccggctgttc acaacgaaga gcgtgtgatt gtccttattg tattcctcgc
     5221 tgccggcttc gtacgcgacg ccgcttacgt tcggtatagc ttccagctgc gctttgattt
     5281 cgggtatctg atcggcggtc agatcatcga acatcacgcg gatcgacgcc gtctcgtcgg
     5341 actcagggaa gacgtcctcc ataatggaaa gcccctgctt catttcggag ctgtccgcaa
     5401 gatattttgt ccgatcccgg ttgatcggaa cgcgcagcgc gagaacggca caaactgccg
     5461 tcagcacaag catgacggca gtgagaacgc ggcggcggtt tataagaaac tctgcaagct
     5521 ttttcatggc gcattaccct tcttgacttt ttccgtttgt ttcattatga tgaacacatg
     5581 ttgattattc aaccgacagt tctttcggta tggaataaaa atcaacacac cgttgggaaa
     5641 tgttgagggt tcaaaaaaaa ttcattttta ggaggctgcc atggagaaaa aagaggatcg
     5701 ccgcattgcg atgacaaagc gcctgctgaa ggccgcgctg atcgagctgc tgaaggaaca
     5761 ggacatatac cacatttcca ttcgggagct ttgtgaaaga gccgacgtga accgaacgac
     5821 attttataag tattacggaa gtcagttcga tctgctgacc gatatggaaa aggatatgct
     5881 ggactttatc gcaaaagcca tcgagcgcca tgaaaccgaa ccggagaaaa tcatcaccgc
     5941 cgcctgccag tatctggagg agaatctgga atttgcccgc ctgatcatca acaacaacgt
     6001 tgacccggca ttcgcccaca agctgtttgc catggacagc ctgaaggaaa gcgcgcgcaa
     6061 aatgctctcc ggcagcaaca gtgaagcgga gcttgcgtat gtctataact ttctgacctt
     6121 cggcgccttc cgcatggtgt gcgtctggct gaacaaggag gagcgggagc cgcccgatct
     6181 ttttgccgcg tttctctgcc gggtgctgta cagcagattc tgattcttcc gcttttgaaa
     6241 gcggcccgct gtcctgcccg cacggagcga gaaaggcgcg gcgggcttta atgtgcttta
     6301 tctggggctg atggcatccc caagacctgc gcctatgtct ggacagtgct gatcggatat
     6361 tccgtcatga aaaaagagtg cggctgaccg agcccggatc ttctgccggc gggcgcgggc
     6421 tctgcgaggc tttttgaaaa aatgagccgc cgcgtcatgc tttgtatgac gcggcggctc
     6481 gttccccaac aatttaacta tatagtgccc ggcggcaaca gtcaagagaa attgcgtgat
     6541 cgccggcaat ttcattgtga tgcacaacgc gggttccgct ttttctccga tcccgtcact
     6601 tgtttccatc ggcggtctct ggtatcataa aagtacaaaa aggaggctaa atgatgcccc
     6661 ccggaccgct ggagtctggg gaaaatgaca ccgaaaaacg cgtgaaagga tctccgacag
     6721 catccgccgt atgcggaaag cctccggcga agcggggcag aaaagcgaag aagggagaag
     6781 atttgcacgg ggaagcagcg gtgtcatgaa tttgaagaaa ggaggtaacc aggatgttag
     6841 atattaagag tgaaagatag cccttgactg cgaatcttct gtaacgcagt caggggctat
     6901 cttttttttt gcgctgaacc gtgagtctgc gccgatgcgg aaaaatcacg ggaaggctgc
     6961 cgataccgga tgcacggtct ctcaccgcgg aacgcgggaa tacaccagcg cgccggtcac
     7021 gttaacgcnn nnnnnnnntt tccgacaggg cgttgaatgt ctcagggttt tttacataca
     7081 agcgatacgt tcccgccgga atttcctcaa tcagcagcag ggatgagccc ccttgctttt
     7141 cctgaaaata cgccgtgccg tcctcggaac ggataccgta ttccaatgcc tgcccgtttc
     7201 tcgcccatgt gatggagagc cataaggacg agtcgttttc ctccataacg atctcaaacg
     7261 gggatacgat ctccccaccc ggggcaagat cggaaaacga aatgccgtcc tgccattcca
     7321 catcgtccga aacgttttcc cgctccctga cgtttccgcc gctctcatag gcgcggggat
     7381 tgaccggctc ttgcgcaata cggatgcgat agaccgtatg gatccgcccc ggatcggttt
     7441 caaggattct gccgtcgtat gtgatctcga tcacgtctcc cacctgcagc gcgggggacg
     7501 gctccatgtt ctccatgggg acctccagca gagccgcgct gttgatcgcc cagccggctt
     7561 ccggttttac aaggaaatac ccgtcgcgga tctcaaggat ctccgcttca aaggtgatgc
     7621 tgtccgggga attttcgtcg gaactacggc cgcgaatctc cgcattttcc actgtcaaat
     7681 atgcggtaat gtcctcatcg gtcgtgttct gcgctttcac gcccaccctg agccagattc
     7741 tcttatccgc gtcaaattcc gcgggcattc cttgggtgag ataaaccgcc gtatagccgg
     7801 tctccatggt ttcctccaca ggggttaaaa ggacttccgt gtcccccagc ccgccgttga
     7861 gccatattct gaccgtattt tttgtcgcac gaaactcttc ctcggagtat acgaatcttc
     7921 cttgggttcc cgccggaata gtgattcgga acgtatatcg ttccggcttc ggccttgtca
     7981 ggaggcagac cgcagtaatt ccgcagacga tgacgatcaa caggattccc cgcaaaacgg
     8041 gctttttgcc gcttgtcatg gctttcacac actccttttt ctttttttgg gataaacaag
     8101 cgtcgattaa caagggcacg cacgtcggac agccagcctt ttcggtgctt tttgcccttt
     8161 tgtccttggc gggcgacagc ccgcctgcgt ccaaaagaac aaaaatccct cgaaaattca
     8221 aggctgtccg atgcgaagca gttctgccgg aaagctccgc acgctttccc gcatcaggtt
     8281 tttgtcgtat agggatccag aaacggcgac ggggatttct tatatgtggc aaggctcacg
     8341 atgaccagcg ccgcagcgca ggcgatggcg cccggtaccg tcgcgccgag accgaagggc
     8401 agcccggcga gcttccagcc gatgcagacc gtgaagcccg cgatcatgcc ggagaggatc
     8461 cccgccgtag tcgccttctt ccagtagagc gcggcgaacg cgggaagccc cgccgccgcg
     8521 gcgtagaagc tccaggcaaa catgagtatc tgataggcgt tcttcatgta cagcgcgatc
     8581 gccagcgcgc ccagcggcat gatgacggca aagacacggg tcaggatcag ttcctttttc
     8641 tcggacatgt ccggatcgaa cgttttatag acatcgtgca cgcagctttg cacggatacg
     8701 atcagatagc tgtccgccgt ggacatgatg acggagagta tcccggcgag agcaagcccc
     8761 gtgagaccta caggcaggat ctcaatggca agcgccggaa cgacggcgtc ggtggttccg
     8821 tacatggcga tcttttccgc gccgatgagc tggtgcgcga ccacgcccat gaaccacacg
     8881 agagggatcg taagcccgta tgccacggtc ccgagaaaca gcccgttctt tgccgccttc
     8941 tggcttttcg ccgcaaaggc gcgctgccac atctccgcgc ccgcgagcgt gaacacgaag
     9001 taggtgatga tatcccctag gatcgtgccg tcgacatagg gcgtcgccag ctccggcgcg
     9061 agattgcgga aaaaggcggc cggcccgccg agctttacaa gagaggcgcc ggggatgagg
     9121 atgtacacga acaaaatcag catgacgaac tgaaacacgt ccgtgtagat cacgccgaac
     9181 agaccggagg tggcggtgta aatgatgaac accgcgcagg cgattaaagc gcccatctca
     9241 taggagacgc cgatctcccc gccgagcatg ttgatgatgg tcgccgtcgc cgtgacctgg
     9301 gccgcgacgg tccccatcat ggtgaaggcg atcagcgcgc cgagcgtgac cttggtggct
     9361 ttgccgaagc gccgttcaaa aaggtcgggg atggaggtga tgttaaagct cgtgccgacg
     9421 gcggatatac gtccggcaaa gccggaaaaa atcagcatcc ccagaagata gggaaccgcc
     9481 gtcataatgg ccttgaagcc gctggaatag gcgacgcccg cccgtcccat cagaccgctg
     9541 ccgccgatga tggtggcgca gacggtcgcc atgagcacca gtgggccgaa ggaccgtccg
     9601 ccgagataat agtcatccgt gttcttgatt tttttgacga caacaaggcc gatcacgatc
     9661 attgcaataa gatatgcgcc gataatggca aggtcgattg cgtgcagttg actatggttc
     9721 attctgcgta ctcctttcct gaccggcgct cgcgccggtt tttattctgt gttgcggaag
     9781 aagcgggaaa ctgcctcccg cgcgttgtcc gtgccgacaa aaatgacctc gtcgccgccg
     9841 tacagctccg cgtcggggcc gggggacacg atacgggagg tgccgcgccg gatggcgacg
     9901 atcgtcgcgc cggtcgcctg ccagaaatga agattgctga gatttttgcc gatgccgctc
     9961 cagttcttcg gaatgcggca gacatagttg ggcagggtat cgtccgcctg tgccgggaac
    10021 gaggcgtagt caaggatcct gcggcacagc tccgcaagct gccttgaaac gcgctcctgc
    10081 tgctccagaa gctccttcat ctgctgacgc ttggcaaaaa cgtcgatttt agccccggca
    10141 ttgtgcagat agcgtctggc attgtccgca gaaagcacat acacgccgct cttctcccgc
    10201 acgtctacaa cgcgcatatc gctcagcagc cgcacggcct tccgaattgt ctccggcgaa
    10261 acgttgtatt cagaggacag cttggagcgc ccggaaattt ttgccccctc cggaacgtct
    10321 ccgcccgcga tccgtccggc aatggagagc gcgatctgca tatattgggg aagcgagtca
    10381 ttttccatcg tttctccctc cctttcatcg gatttctgta cgtatagcat aacatctgac
    10441 aactctttac gcaatacaag tttttagttt tttctctgta caggggtaga attctccgcc
    10501 cgattttgat atgattttta tataaaaaga cagtttccat tcaggaacgt aaacaagcgg
    10561 acgaaaggag agtttccatg gagcgtatca taaccatcgg ccgcgaatac ggcagcggcg
    10621 gccgcgaaat tggccggcgg cttgcggaaa agctgcagtt cgcctttttt gactaccaga
    10681 tcgtgacgga gctggcaaaa cgaaccgcct tcgctgagga gtacatcctg agcatccagg
    10741 aaaagcgccc gtatcccgtg ctccccgttc ccaccgtccg catgtgggcg ccgccgaacc
    10801 cgatgctgga tcagcatctt gaggtttttc tgaaggagag tgaggtcatc cgcgacatgg
    10861 ccgacaaggc tccctgcgtg atcgtcggcc gctgcgccga ctatgttctg cggcaccgga
    10921 atcccctccg gctgttcttt tacgccgaca tcgccgaccg gatggagcgc tgccggaaaa
    10981 gaaacgagga gcagaagtcc ttgaaccatg acgtgctccg caagataatc acgcagacgg
    11041 acaaaaaccg ttcgcgctac tatgagtttc tcagcggtca gaagtggggc gcgaaggaaa
    11101 actacgatct ttgcatcaac acctcctccg ctgatacgga aaagattatc caatgcgtga
    11161 tctcgcttct gtgaagcgtc gattgacatg cgatcgcggt ggggagcttt cccaaacgca
    11221 gcaaaagcgc agcccagatc ctccgcggtt tgcatctgca aagggtctgg gctgcgcttt
    11281 tttcggctcg ccgccgggcg acgggctccc tgcccgcttt ctatcctgcg gcctgcatca
    11341 gcacacacgc cgtcgtactt tgcgacataa acggcagaca gaggctgttt tcaccggcgc
    11401 cgatcagcag ctcctcgccg ctcgggctga tcaggagcgg ataggtttcg cacagatagt
    11461 acggtccgtc cgtgacgccg tagctgacgc cgccgacgag agacatgctg accgtcacat
    11521 agctcggccc atcgagaacc gtttccatcg tccagaagcc gctccacatc tcttcaaaat
    11581 catccgccgc ctcatacgtc cagttcagct caaccgcgcc gcccgtttca cccccgggat
    11641 agaagcgcag cgagaaatcg gcgtaagcgc cggtctcccc cgccatcgta tgcgtatacc
    11701 atcccgtgcc cgtgctctgt gatcccgcca gaccaagcgc cgttatgccc gaccagccgt
    11761 cggcgagcca cggggctaaa tactgcggtg cgttctgcca agcatattcc gtaaagtcaa
    11821 acaatacatt gtctccggct tccgataggc acggccggat ctgaccgcag cttatcgcgc
    11881 tgtcatagct cgccgcatcc actgccggat accattcaca ggtatttccg ctgttgtcgg
    11941 tgataaggac ctgtgtgtca gacacatatg ccatgccgtc aagattggca aagagcagca
    12001 ccggctcgcc tgtttccgag cggtacagaa cctcggagat ttcgttgatc tgtttttcat
    12061 cgttccacag catacggttg atcgaaaccg ttgcgttttc atcgaccgga acgatgcagt
    12121 aaagatgtcc cgcgccgcct gcgatgtggt tctcgtcgat ctccgcaata aagggatacc
    12181 ggcccagcat cgcctcgttc gtttcccgga gccatgcggg aaagcccgcc gcaaagccct
    12241 cttcaaagag ccctccgacg cagccgaggt acgcagcgcc gaacatcgtt gccggaaagt
    12301 cgatcctgtc gcgcagaaat ccaagcgcgg tctctgcctc cggcgaaaag gcctgccgga
    12361 ccggctgcgc gctccaatcc tcaccgcgcg gcgcaggctc ctccggcagc ttatccggcg
    12421 ttcttccgcc ggtctttgtt tgcggcacat cgccggcggc gttgtttatg gctttctccg
    12481 gttcggtttg ccgctcctgc gcgcatgcgc agagagtcag cagcaaaaca agcgtcatca
    12541 acagggcgat cgtttgcttg caggaaattc tcatagccgc tcccttccgt taattccgct
    12601 ccggcgggaa agcaatcttc cgtttctccg cgctgcaatg ctttttcgcc ctcgctctgc
    12661 cggccatatg aactgcggcg cacccgccgc cccgaccgca ggcggcaaga gcgagaaggc
    12721 tcaggcggag acgtattgcg gcgaatgtcc ggttcatttt tcgcccgcgt ttttcatgaa
    12781 tagccgcata gctcccgccc cgaatggggc gggagcgtgc ttttgctcgg tgtcagccat
    12841 cccccctgat atgagggcgg cggcatggag ctttgtcccg cggtctgcgg tgtacggaat
    12901 tatttaatcg tcatgttgtt gagaatgatg tcgggcatcg ttcccgggac gcactccaat
    12961 ttcgtcagtc cgatggacag tgcgcggccg tcgtcgatct gcgcgacat
//
LOCUS       DAWAAE010000048        12957 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2164_length_12957_cov_2.949233, whole genome shotgun sequence.
ACCESSION   DAWAAE010000048 DAWAAE010000000
VERSION     DAWAAE010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_12957_cov_2.949233"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1571..1580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2530 a         3995 c         3936 g         2486 t
ORIGIN      
        1 agataaactg tttatgtcat tcaggctcat tctgaatgcc ctccttttgc tttttaagtg
       61 cagttggcag accgcacgct tattctaaag caaaaggagt ttttttgtct ccctcatcgc
      121 caaaggctct tttgaaccac gcgtctaaca cgtggttttc ggatatacag caaaggcaga
      181 cgtttttcat cgtctgcctt tgccttttaa taattgtttc aattacagca tggtctcgtc
      241 gtacatcgct ctcgcgccgc cgatgaattt ggggcgcgtg cgcgccgcga cgaccatcgc
      301 cgcgccgatc gtgtggtttt ccagccgcac cggcaccgcg acctttttga ggtgcatgcc
      361 gatgagcgtg aggccgatat cgagcccggc gtccgccttg atctcctcaa gcgcgacggg
      421 ttctttgagc gcggcgtaca cctgcgtggc aagcgagccg cccgccttcg gctgcgggac
      481 aacgttcacg atctccgcgc ccggcaccgc cgcgcgctcg gtgatgatgg cgcggttcaa
      541 gtgctcgcag cactgcaccg cgaggaaaat accgcggctt cccgtcacgt caagaagcgc
      601 gcgggcgatc tcccccgccg tttcgacgct ggagtttgtg ccgatcttcg agccgacgac
      661 ctcgctcgtg gagcagccca ccacaaggat gcttcccggc tgcagatgcg ccctttcgca
      721 gatctcggca gcggccgccg ccgcctgttc atataccgtc atattttccc tcctttacgc
      781 ctgtttgata acacggctct tttccagcgc cttcacaagc agcacgccga ggataagtcc
      841 cgccgcgccc tgcacgccgt tggccggaat gttcaccgcc gacggcacga agccgtacag
      901 gcagccttca aaaagatagt agccgagcac catccacagc tccgccgccg cgccgctcaa
      961 aatgcgcgcc gtcaggctgc cggtcttcgc gctgagcgcc ttgaagccgt aaaaggcgat
     1021 gagcgccatg agccccttga tggcaaacgt tgcgggcgca taggcggcat accccgaaaa
     1081 gaggtccgcc agccctgagc cgagccccgc ggcgagaaag ccgtagccgg gcgcgagcag
     1141 ccacccggcg gcgagcacca cgcagtcgcc gagattcaga tacccgtcca tcggtgaggg
     1201 gatcttgatg accatcgtcg ccacacacgc gagcgcggcg aaaagcgccg ccgttacgat
     1261 ctgttttgtt ttaccgttca atggaatcga cctcccggaa tcattggttt ctatgcccag
     1321 tgtataggca aatctggtta tacgcaatta ccaaatagtg ataattttat ataaccagat
     1381 tgggaggcca atattttgtt tcagcggacg agcgccgcgg cgaagtcgtt cacgttcgta
     1441 cccgtggggc cggtgatgat gagatcgccg gtgtcgcgca gcgcgtggta ggcgtcgtta
     1501 ttttggagca cgtccgccac ggtctttcct gccgcggcaa ggcgctcgac cgtgccgccg
     1561 tccgcgaggc nnnnnnnnnn ctcctggttg cgcccgccct tgccgttccc ggtgagccgg
     1621 acgaccgttt cgccgccgag gataaaggcc cgcggcgtgc cgcaggacgc tttctcccgc
     1681 gcgagcgtgc cgaggcgcgc gccgacctca cgggcgacgc cctgctcaca gtcggtaaga
     1741 atttccggcg tatacccaag atcgcggcag gcttttgccg cggcggcgca cagttcgcgg
     1801 acgctgccgg tgacgcgcac cgttacgttg tccgccgttt tcggcgtctc ctgcgcgagg
     1861 agtccccgcg cggtatcgct cagccggaga gcgtactttt ccgcggcggc gagcgcatcc
     1921 gcgcacgtgg acgagtccgg cgataccggc cccgaggcga tcatatccac cgggtcgccg
     1981 acgatgtcgc tcaatatcac ggcgaacaca tgtgccggag cgcaatgacg cgcgaaccgg
     2041 ccgcccttca ccgccgagag gcgtttgcgg atcgtgttga tctccacgat gtccgcgccg
     2101 caggcgagaa gctgcgcggt gatgtcctgc aattccgcgc ccggaacgag cgggctttca
     2161 aaaagcgcgc tgccccctcc ggagagaaga aagagcaccg tgtcgtccgg cgtgagattt
     2221 tcggtgagcg caagcgcctc gcgcgtggcg gagaacgagt tttcgtccgg cacggggtgg
     2281 cccgcctcgc ggcagacaat gccggggatc gtcccctgcg catggccgta tttcgtgatg
     2341 acgatgccgg tatcgatccg ctcgccgagc gccgacactg ccgccgacgc catgcgccac
     2401 gccgcctttc ctgcagcaac gaggatcacg cgccccggaa agtccgcccc gtcgagcgcg
     2461 cgccggacgg cttcatccgg cagcacggct tcgattgcgc gggtatagat atacttcgcg
     2521 tcctcgcgca gcgcacccat gagccgatca catcctttgg aagtgctttt gcaaaaagta
     2581 tagcgccgtt ttcgtcgttt gtaaacaaat ccggccgcaa aaatgtgcgt tgacaaaacc
     2641 cgccgcaggg catagagtgg atttgaattt aaaaagccgt aaggaggaca cgcacttgaa
     2701 gctcaacgcc agaaaagacg taccggtcaa cgagacatgg gatctctccc tgattttcgc
     2761 agcggaagcc gattttgagg ctgcggtcga aaaagcaaag gcgcttgccg acacgctgga
     2821 gaaaacgtac aaaaacgctc tcaccacgcc ggagagcatc gccggatgcc ttgcgctcta
     2881 tgaggagctg gagatcctgc tctaccagac gacgagctac acgagcctcg ccgtttccgt
     2941 agactatacc gataccgagg cgcagaagaa ggacgcgaag atgaccgcgc tcgccgccga
     3001 gatcggaagc cgcctctcgt tcatcgagag cgagatcgcc gacgcgcccg aagagctcat
     3061 ccgcgccgcc atggacaaga ccgggcgcgc gaagcattat cttgcggaga tcctccgcga
     3121 gaagccgcac cgtctcagcg ccgagaccga aaaggtcctc gccgcactca gtccggtatt
     3181 caacgcgccc tacgacattt accacatgac gaagctcgcc gacatgaagt ttggctcctt
     3241 caccgtgaac ggcagggaat acccgctcgg ctactcgctc ttcgaggacg agtatgagta
     3301 cgaggccgac acggacgtgc gccgcgcggc gttccgcgcg ttctccgata agctccggga
     3361 gtacgagaac acgaccgccg ccacctacaa cacctatctc acgcagcagc gcattatggc
     3421 caagcagcgc ggctttgccg acatgtttga agccgacctc ttcaccgacc acgtcacgcg
     3481 cgagatgtac gaccggcaga tcgacctcat caccgaaaag ctcgcgcccg cgcaagtacg
     3541 cccgcctcgt cgggaagatg aacaagctcg accgcgtgac ctttgccgat ctcaagctgc
     3601 cgctcgacgc ggagtttgac ccgcgcgtca cgatcgggga atcgcgcgaa tacgtccgca
     3661 gcgcgctttc cgttctcggg caggactacg ccgacatggt ggacgaggcg tacgataagc
     3721 gctggatcga ctttgcccgc aacgtcggca aggagacggg cggcttctgc tcctcgccct
     3781 acggctgcaa ttcctacatt ctgctcagct ggaacaaccg catggcagac gtgttcacga
     3841 tcgcgcacga gctcggccat gccggacact tccggctgtg caacggcgcg cagtcgctct
     3901 tcgatacgaa cgtctccggc tatctcattg aagcgccctc cacgatgaac gagctgctgc
     3961 tcgcgcagga tctcctgcac aagaacaccg acaagcgctt ccgccgctgg gtgctctcct
     4021 cgctcatcgg gcatacctac taccacaact tcgtcacgca cctgcgcgag gcatggtacc
     4081 agcgcgaggc aatgaacatc atcgagcagg gcggcgcggt gaacgccgaa acgctctccg
     4141 ccattttccg caagaatctc gaaacgttct ggggcgatgc cgtggagctg accgagggct
     4201 gcgagctcac ctggatgcgc cagccgcact attacatggg gctctattcc tacacctaca
     4261 gcgccggtct cacgctcgca acgcaggccg cgctcaacat cgccgccgag ggtgagagcg
     4321 ccgtggcgcg ctggcgcgcg atgctcgaag ccggcagcac gcgcgatccg ctcggtctcg
     4381 ccgccattgc gggcatcgac ctctccacgc cggacgcgct tgagcacacg atcgcctaca
     4441 tctccgatat catcgacgag atcgccgcgc tgacggagga gatcgacggc atcacgctgg
     4501 attgaaaaat ttttcaaaaa acgggcgctg actttatcgc atttatgtgc taaagtttgg
     4561 ccatcaaacc gaagagcaag ataagcagcc cgccaatgag acgtctttca gagagccgcc
     4621 gatggtgtga atgcggtaag aagtttccgc gggcgaatgg ccttgcgagg gcaaacagaa
     4681 agggagcgta tcccgagtat gggcgacgga gttggctgtc cgttatccta aggccggcgc
     4741 atgaagtgtg cgtttaagtg ggcgcgaaag cgtcaagccg ggtggtaccg caggagttta
     4801 tccaagctct tgtccctgca tttgcagaga caagagcttt ttctttttcc tgtctctgct
     4861 ccaaaaagaa aactgacaga aaaggagcaa aacaccatgg aaaaccggaa catcccctac
     4921 aagatctatc tcgacgaagc cgatatgccg caggcatggt acaatctccg cgcggacatg
     4981 aaacataagc ccgcgccgct gctcaatccc ggcacaggct acttcgacat gaccgcctat
     5041 caggcgttca acgacggcaa gatgaccgac tatatcccca ccgatgcaga gcttgcgcag
     5101 agcatcgcca cgctcccgaa agtccccggc acggaataag aaaacgatat gacaacagca
     5161 gccgcggcta taaaagccga ggctgctgtt gtttcatggg tttttcagat tgacacagcc
     5221 gtttttgcac cggaagccgc ttcgggatcg ctcagcgtca tagcgttttg gaatagccgg
     5281 atctcgtcaa ggtatatgtt taccgtctat cgcgctttcg ctttttcgat ttcttttttc
     5341 cgaaacgtcg agacagcatc aggtatgaca aaaagcctac ttccgcggcc agcaaggcga
     5401 tcagccacca gccgactttt agatcaaaga agatcatcaa gctcacaacg ggaagcagag
     5461 aaagaattat tccccaagta tacgaatcgt cctgcatggt cggtcgggga tcgttcatgg
     5521 caatgggtgc catagcggca aaaagaatcg tcatcaagat atactgacca aagacttcgc
     5581 ggggtaagga tcgctgtgga ataagggcaa cggcagaatt cagcgcgcag aagccaaaac
     5641 cggcgcagat aatatacttg ttctgtattc ttttcatatc ggaacgtctc cccaaggaag
     5701 cctctattgc tttttaaata acagtatatt ccaaaatgaa tttttcgctt cagcgctcat
     5761 cccctcatac acaatattac tatccaaaga tgttgaaatc aacataaagc cgtgtgaaat
     5821 cacaacgaaa aaatgtaatt cggcaaacaa ttctctaaaa aattgcaaaa aggaacgcct
     5881 tttgctttcg caaaaggcgt tcctttttgg cggagaagcc gggatttgaa cccgggctcg
     5941 gctcatcacc gactactccc ttagcagggg agccccttcg gccacttggg tacttctcca
     6001 aaccgctcga gtatcatatc acagggtatg cggccttgtc aatgcaaaaa atcagtccag
     6061 cacggatttt acggcggaaa gcacgtccgc ggcgacctcc tcgggcgtgc gggcagcgtt
     6121 cacgatgcag atcctgtccc gatgctccag ccgccggaag atctcataat accgccggcg
     6181 cacggcgagc agggaatttt cgttctcgta gatctcgcgg taagaccgcc cctgctccat
     6241 gcggcgcagg cactcctcgt cgtccaaatc aagaaacacg cagagatcgg gctttcggat
     6301 ctccgggcag tcgatattga tacggaacac ccattccggg tcggtcacgg tgccctggta
     6361 ggcgagcgag gaatagtaat accggtcgca gaggacaacg cgcccctccg agaggagctt
     6421 tccgatgccg ttgatggggt ttacgttgtg cgccacgcgg tcggccatga agagcgccgc
     6481 aatctccgcg ccggtgcgcg gcgtgaagcc ggagagagcg tcgcgcgcga gcccgccggt
     6541 cgtgctcgcc gtcggctccg cggtctccgc cacggcatag ccgagcgcgc gcaggttttg
     6601 ggcaagcgcc gtcagctgcg tggttttccc cgagccgtcc agcccttcga tgacgataaa
     6661 ttttccgttc atggcagttc ctcccgccgc gaggcgcgat ttgttcgcag atattatagc
     6721 aaaactcttt tctcgcggca agaaaaatga tataatatat tttgtattat tgtctttatg
     6781 gagcgaatgc atgacagaaa ccgctttttt acaatacacc gggtatgtgc tcgcgttttt
     6841 cgctgtttgg atcgtggcac gctgcgcgcg ctcgatgctg agcgaaaggt atgtgccgga
     6901 ggtatggggc tatctggagc tgccggacgg cacatcgcgc gcgatcgagc actgggagtg
     6961 cattctcggc cgggcgaagg cctcggacat cgtgctcacc ggcgagggga tcgagcgcag
     7021 ccacgccgcc atccagcgct ccggcgcggg cgagtggacg ctctccgcgc tcgacggcgg
     7081 caggggcgtc tggctgaacg gcaaggccgt cacgggcgac gcgccgctcc gcggcggcga
     7141 tacgatcaag cttggcgaaa tggagctgca ctttctcgcg ctggacgacg cgcagcgcgc
     7201 cacattgcag cgcaagcgcc ccctgaccgg gcacaaggtg cagccggcgg tgacgcttct
     7261 tctgctcgta tgcttccagc tcgtgctgct cttccagttt ttcttcacga cggacggcgc
     7321 ggcgctcgcc tacatcgttc tctcgttcgg actgctcatt gcgatggaat ggttctgcta
     7381 ttttgtgatg cgcagcattt ccgtgcgcgg gtttgaaccg gagacgctcg cgtttttcct
     7441 cacaagcgtc ggcttttccg tcgccgggtc ctccgcgccg gacggtatgg ggcggcactg
     7501 tctcctgttt ctcgcggcgc tcgcgctttt ctttgcgctt ggcgtgtggc tgcgcgatct
     7561 gcaccgggtg cgcgcgatgc gttggccgat ggcgtttgcc gcgctcggct ttctcgcgct
     7621 caatctgctg ctgagcgaaa cgatctgggg cgcaaaaaac tggctgagca ttgccgggca
     7681 gacgctccag ccctcggaat ttgtcaagat cgcttacgtc tacgcgggcg cggcgacgct
     7741 cgaccgtctg ttccggcggc gcaaccttat cctgtttatc gccttctccg ccgtgtgcgt
     7801 gggcgcgctg gcgctcatgg gcgatttcgg cacggcgctc gttttcttcg agtgctttct
     7861 cgttatatcc ttcatgcgct ccgggagctt tgccacgctc tttctcgcca tcggcggcgc
     7921 ggcgctcgcg gtgatgctcg ttctgaccgt gaagccgtat gtggcgcagc ggtttgccac
     7981 ctggggccat gcgtgggagg atcctctcaa caccggcttt cagcaggtgc gcaccatgtc
     8041 cgcgacggcc gccggcggtc tctttggccg cggcgcggga gcgggctggc tcaaaaacat
     8101 attcgccgcg aacaccgaca tggtgttcgg cgtcgtgtgc gaagagctgg ggctcatcgt
     8161 cgcgctgtgc tgcgtgctct cgctgctgct gctcgcggtg ttctccgtgc gcagcgcggc
     8221 ggcggggcgc tcttcctatt acgtcatcgc ctcctgcgcc acggtcacga tcttcatggc
     8281 gcagctcgcg ctcaatgtgt tcggctcgct cgatctgctg ccgttcacgg gcgtgacgtt
     8341 cccgttcgta tcgaaaggcg ggtcgagcct catctcctgc tggggcatgc tcgcgtatat
     8401 caaggccgga gacacgcgct ccggcggcag ctttgcgctg cgcacgagct ttgaaaacgc
     8461 ccgccgcggc aaaaagaaaa aggaggcggt gaaatgaaac gcattgaaca ccgctccgcc
     8521 atcgcgctgc tgcttgcggc ggcgctggcg ctcggcatgg gcttttttgt cgtccggctg
     8581 gctctctgcg gcggcgactg ggcatcttac agcgccaatc agcacattta ccaaaacggc
     8641 gtgctcaaaa gcagcacatt gaccgaccgc aacggtgtca ttcttgcgca gaccgaaaac
     8701 ggtgcgtggc gctacgcgga cgatcctgcc gtgcggctct cctccgtcca cgccgtcggc
     8761 gactaccgcg gatacatcag cggcgcgatc tccgcgtttg ccgatgagct tgcgggctac
     8821 tcgtttgttt acggcacgcc cggcggcggc acagaagtcg cgctctcgct cgatacgaga
     8881 ttgcagaccg ccgcgtggga cgcgctcgcg ggacgccgcg gcgcggtgct cgtgatgaac
     8941 tacgagaccg gcgagatttt aacgatggtc tcctcccctt cctacgatcc gaacggcgag
     9001 gccgacacga gcatcgacgg tcttttcatc aaccggtgca cgctcggcac gttcacgccg
     9061 ggctcggtat tcaagctcgt gacgctcatc gccgcggtgg agaacctccc cgatctcacc
     9121 gaacagacgt tcacctgcaa tcagtatctc gatctccccg gcgggacggt cgtatgcacg
     9181 ggctggcacg gcccgcagac gatcgagcag gcgctcgcaa attcctgcaa ctgcgcgttt
     9241 ggggagcttt cgcttgagct tggcagcgag acgatcgtga aaacggcgca gcggctcggc
     9301 gtggccgggt ctctcacgct cgacggcgcg gccacggcct ccggcaagat cgagaccgcg
     9361 gagcccggca gcagcgacga ggcgtggctc ggcatcgggc agtacaccga tcttgtgacg
     9421 ccgtattcga tgctgcggct gtgcgccgcc attgcaaacg gcggcaccgc cgttacgccg
     9481 acgctcgcct atggcggcgt gggcggcggc gcggtgctca tggacgcgga gacggcggag
     9541 tatgccggcg agtgcatgag ctacgacgtg gcatacggct atggccgcga ccggtttccg
     9601 gggctggatc tctgtgccaa gagcggcacg gcggagatcg gcgacggcac gacgcacgcc
     9661 tggttcgtcg ggtatctgcg caccggcgcg ccggtggcgt ttgcggtgat gctcgagcac
     9721 ggcggcggcg gactgctgca ggccgggggc gtcgccaatt ccgtattaca gactgtaatg
     9781 gagtattatt ccgatgattg acatttctgt ccgggacctc acgaaggcgt ttgaggtcaa
     9841 caacaacatt ctcgacgggc tgacgtttca ggtacagcac ggcgagcgcg tcggcattct
     9901 cggccgtaac ggcacgggga aaacgacgct tttccgcttg atggtcggcg agctcaccga
     9961 ggacaagggc gagatcgaca ttgcccgcgg caagcggctg gggcttgttt cgcagatccc
    10021 gcgctatccg gcgggctaca ccgtggagga cgtgcttgcc acagcggagg agcggctcaa
    10081 cgcgatgcag gcgcggatgc gaaagcttga agagctcatg gcggtatcaa gcgaccgcgc
    10141 gctgctctcg gaatacgacg agcttctctc gcgctttgaa atgctcggcg ggtacgacgc
    10201 cgaggtgcgc cgcagcaagg gtcttgacat cccccctgcc atgcgcgggc agcttttcga
    10261 ctcgctctcc ggcggcgaga aaacgcgcgt caacttagcg cgcctcattc tggaggacac
    10321 cgacattctc ctgctcgacg agccgacgaa ccatctcgac atgcacgcga ccgagtggct
    10381 ggaggactat ctgctccact tcaaggggac ggtgcttgcc atatcgcacg accgctggtt
    10441 tctcgacaag gtcgcgcagc ggatcattga gatcgtcaac ggcaaggcgg agttttataa
    10501 cggcaactat acgttctatg ccgaggaaaa gcagcgccgg tacgaggagc tgcgcaggca
    10561 atatgagaaa aaccagaagg agatcgccca tctgcagaag gcggcggacg atctgcatct
    10621 gtgggcgttc atgggcgcgg acaagctgca caagcgcgcg ttctccatgg aaaagcgcat
    10681 cgcgcggctc cagaccgccg aaaagccgcg cgaggacaaa aagctctccg tccgcttccg
    10741 gcagaaggaa tttttcggcg acgaggtgct tgtgctcgag gacgtggcga agtcctacgg
    10801 cgagaagaag ctcttctccg gcatcgacga gctcatagag ccgggcgagc gcatcgcgct
    10861 catcggcgac aacggcacgg gcaagtccac gctgctccgc tgcatcaccg gtgaggaaac
    10921 gccagaccgc ggctggatcc atctcggccc cgcggtaaag agcgcgtacc tgccgcagct
    10981 cgtgcggttt gacgatccct tgcgcaacat ggtggacacg atgctctggg aggcgaagtg
    11041 cgatccgcag acggcgcgaa actgtctcgc ggcgttcggc ttccggggcg aggatgtgtt
    11101 caagaccgtt tcggtgctct ccggcggcga gcagagccgt ctgcggctct gcattctcat
    11161 gcgggacgac atcaactttc tcatgctcga cgagccgacg aaccatctcg atcttctctc
    11221 gcgcgagtgg atggaggatg cgctcatgga ctacagcgag gcgctgctct tcgtgagcca
    11281 cgaccgctgg ttcatcgaga agtttgccac gcgcatctgg tgtctgcacg acgggcagat
    11341 cgaggactac cgcggcggct ttgccgagtg gcgcgaatat aaagcgcggc aggaagcgct
    11401 ccagccgccg gcgaaggccg caaagagcgc ggcgcgcgag gagaagccca agaaaaagac
    11461 cgagccgaac cgcgaccgcc gccgccagaa gatcgagcgc gacattgagc gcgccgaggc
    11521 gtcgctgcgc gatatcgagg ccgaaatgga gaaaaacgcc tccgattacc agaagctgct
    11581 cgagctcggc gcgcagaaag acgccgccga ggaatcgctc gggacgcttt acgccgagtg
    11641 ggaaacgctc gcggaataat ctacgaaggg agagaagaac gtgccggagc tgtctacgga
    11701 tgtccgttac ataaaaggcg tcggcgagca gagagcaagg gctctcgcgc gtctcggcgt
    11761 gcgcacgctc ggcgatcttc tcaactattt tccgcgcgcg tacgaggacc gcaccgccgt
    11821 gcggcccatc gccgagcttg ccttggacga aacggcgtgc gtgcgcgcca tggtcgcagc
    11881 ggagccgcgc ctcacgcgcg tccggcgcgg gctcgatctc gtgaagctgc gcatcgtgga
    11941 cgagagcggc agcgcggatg tgacattctt caaccagagc tatgtccgcg accagcttgt
    12001 tccgggcgaa agctatgtct tttacggcaa agcgggcggg aatctcctgc gcaagacgtt
    12061 cacgaacccc gtttttgagc gcgaggaccg cgcgggcacc gtcacggggc ggatactgcc
    12121 gctctaccgg ctgacgcacg ggatatcgaa taagctcgtc gtgagcgccg tggaatgcgc
    12181 gctcaatgcc tgcggcgata tgctgccgga gcttctgccc gcagagatca ccgaacagta
    12241 tgcattgccg aaggtcggct ttgcgtaccg gaacgtgcat tttcctccgg attgggaaac
    12301 gctcgctctg gcgcggcggc ggctgacatt tgaagaattt ttcgttctct cctgcgccac
    12361 gcagcggctg cgcgcgcagc gcgagagtgt gcccggcgag cgtatcccga cgccggacgt
    12421 taaagagttt tatgccgctc tccccttttc accgacgaga gcgcagcggc gcgccgcgga
    12481 cgaggcgctg cgcgatatgg cgtccggcaa gcggatgtcg cgccttttgc agggcgatgt
    12541 cggcagcggc aaaacgcttg tcgcggcggc gcttttgtgg gcggcacagc gggccggata
    12601 ccagagcgct ttcatggcgc cgacggagat tctggcgcag cagcatgtaa aaaccctgca
    12661 gggctttctc gcgccgttcg gcatccgcgt atgccttctg accggctcga tgaaggcaaa
    12721 ggaaaaaagc gcggtcaaag ccgcgctgga ggagggcgtg tgcgatgtcg ccgtcggcac
    12781 gcacgccctg ctcacggagg acgtgcgctt tcacaatctc ggtcttgccg tcacggacga
    12841 gcagcaccgc ttcggcgtgg aacagcgcgg cgcgctcggc gaaaagggcg cgcagccgca
    12901 cattctcgtc atgtccgcca cgcccatccc gcgcacgctc gcgctgatga tctacgg
//
LOCUS       DAWAAE010000049        12741 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2216_length_12741_cov_3.313180, whole genome shotgun sequence.
ACCESSION   DAWAAE010000049 DAWAAE010000000
VERSION     DAWAAE010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_12741_cov_3.313180"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1711..1720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2369 a         3758 c         3927 g         2677 t
ORIGIN      
        1 aactggattt acgcagaaat gccacacgct gtagctttat tataacggat tcggatggga
       61 atttcaaatg ctttttgaat gactttggtc tctcctgatc ttggggcaat tacaatgctg
      121 ccggataacg caaaaaatcg gcacctgcct gaaagcaggt gccgatttca gttaccctat
      181 tatgcgcttg gatttttggc ataagtttgg cgtaaatccg ctttggcaca ctcaaaaatt
      241 catgcaatta caaggctttg cggtgtggct tagtacatac cgcccatgtc cggcgcggcg
      301 ggagccggcg cggggggttc ggggatgctc acgaccatcg cctcggtggt gaggacggtg
      361 gaggcgacgg aggcagcgtt ctccagcgcg ctgcggcaga ccttggtcgg atcgacgata
      421 ccggcggcga tcatatcgca gtactcttcc ttcgcggcgt caaagccgta gccggggatc
      481 tcggagccgc ggacctgatt gaggatcacg gcgccctcga gaccggcgtt cgcggcgatc
      541 tgcatcagcg gagcctgcag agccttcgca acgatggcag cgcccgtctt ctcgtcgccc
      601 tcgagctcgg tgacgacctt ctcggcagcg atgccggcct gcacgtaagc cgtgccgccg
      661 ccggcgacga tgccttcctc aacggcggcc ttcgtcgcgt tgagagcgtc ttcgacgcgg
      721 agcttcttct ccttcatttc ggcctcggta gccgcgccga ccttgatgac ggcaacgccg
      781 ccgctcagac gggccagacg ctccatgagc ttctcacggt cgtagtcgga ggtggaaact
      841 tcgtactggt tcttgatctg cgccacgcgg tcggcgatgg cttccttcgt gccggcgccg
      901 ccgacgatga tggtgttctc cttggagacc ttgacctggc gcgcacggcc gagcatatcg
      961 aacgtggcgt ccttgagctc catgccgtag tcgccggaga cgacctggcc gccggtcaga
     1021 acggcgatat cctgcatggt ctccttgcgg cggtcgccga aggccggggc cttgacgcac
     1081 acgcaggtga aggtgccgcg cagcttgttg agaaggatgg tggcgagcgc ttcgccctca
     1141 acctcgtcgg cgatgatgac aagcttcttg ccggccttca cgatctgctc gagcaggggc
     1201 aggatctcct gaatgttgga gatcttctta tcggtgatga ggatgtaggc gtcatcgatg
     1261 acggcttcca tcttctcgga atcggtgacc atgtaggggg agacgtagcc gcggtcgaac
     1321 tgcatgccct cgacgacctc ggaataggtc tgcgcggtct tggactcttc cacggtgatg
     1381 acgccgttct gaccgacctt ctccatagcc tcggcgatca gggtgccgat ctgctcgtcg
     1441 ccggaggaaa tggtgccgac gcgggcgatg tccttcgtgc cgctcacggg ctgggacgcg
     1501 gtcttgatct cactgacggc ggcctcggtc gccttggcga tgccgcggcg gaggaccatc
     1561 gggttggcgc cggcggcgag gttcttcaga ccctcgcgga tgatggcctg ggcgagaacg
     1621 gtcgcgctcg tggtgccgtc gccggcgacg tcgttcgtct tggtggcgac ttcgcggatg
     1681 agctgcgcgc ccatgttctc ggcgggatcg nnnnnnnnnn gatcggcgag ctcgatctcc
     1741 ttggcgatgg tcacgccgtc gttcgtgatg agcggggtgc cgaatttctt atcgagaaca
     1801 acgttgcggc ccttgggtcc gagcgtgatc ttcacggtgt cggcgagctt gtcaacgccg
     1861 gtctgcagag ccttgcgggc ttccgcgcca taaaccagat tcttagccat aatatattcg
     1921 tcctcctgaa attactctac gatcgccaga atgtcgctct gacggacgat ggtgtattcc
     1981 tcgccgtcga gcttgacgct ggtcccgctg tacttgccgg cgatgacctt gtcgcccgcc
     2041 ttgacttcca tcttcacttc gttgccgtcc accattccgc cggggccgac gacgatgact
     2101 tcgtaaactt ccggcttctc ctgcgccgcg ctcgtgagga tgatgccgcc tttggtcttt
     2161 tcctcggctt ccgtccgctt gaggacaacg cgatccgcca acggtttgag attcatggtt
     2221 tctgcctcct atgatcattt taatttttat tttgttgatt tagcactcaa tgactccgag
     2281 tgctaaccca tgtctataaa atagcacacg ccctctgcaa atgcaagagg gaatgtgctg
     2341 ttttaaaaaa taattcacat attacgggaa acgggatatc acgggatatt aaaggtacat
     2401 ataaagctgg caggggatca tgccgttata aagtctccgc cgcttgtcgg cgcgcttgcc
     2461 gtagacattt tcaaaattct caagcgagga gatcacgtag gtcttccagt tttccgtttc
     2521 gcgcatcgtt ttgccgaacg cgcgcatgag ctcgaccgcg gcctcctttt cgagcaggcg
     2581 ctcgccgtag ggcgggttgg tgcagagcgt gccgttctcc gttttgcggg agaaatctct
     2641 tgcgtcggcc acggagaagt caatgtactt ctccaccccg gcgcggcggg cgttctcgcg
     2701 cgcgagagcg acggcgcgcg ggtcaatgtc ggaggcgaag atgtgatagt cgccggaata
     2761 ttcgcggctg cgggcaagat tccgctccgc ctgccatatg ccgcgcggca ggcagcgcca
     2821 cgtttcggcg gcaaactcgc ggttcagccc cggcgcgcgg ttgagcgccg cgagcgcggc
     2881 ttcaatggcg atcgtgccgc tgccgcagaa ggggtcgcag aaatcaccgc ggccgcggta
     2941 gcgcgcaatg tccacgatgg cggcggcgag cgtttcacgc agcggagccg cgacctgcgc
     3001 cgggcggtag ccgcgcttat ggagccccgt gccgctcgta tcaatataga gcgcggcctc
     3061 gtccttcata atggcgaact ggatggggaa gctctcgccc tcttcggaga accacgagag
     3121 accgtacacg ctcttgagcc gttcgacaac ggcctttttc acgattttct ggcagtccgg
     3181 cacggagtgg agcgcgctct caagcgccca gcccttgacg gggaacgcgc cgtccgcggg
     3241 gatgaaagcc tcccacggca gcgcgcgcac gccctcgaag agctcgtcga aggtgcgcgc
     3301 cgggaaccgg ccgaggacga tcagaacgcg ctcggcaaag cgcgagcgga tgttcgcgcg
     3361 cgcgacggcg ctctcgtcgc cggtgaagta tacgcgcccg ttttccggca caacattttc
     3421 catatccata tggcgcagct cgttgccgag cggaccttcc agcccgaaca gacacggcgc
     3481 gcagagagtg agtttcatgt tatttcctcc gatgtgatct ctattcggaa agatagtacc
     3541 ctgtttcgct ccaaattgca agcaatataa aaaccccgcc gtttccggcg gggcaaaaag
     3601 agagaatgag agagggtagg tatttaaagg gtaggtattt aaaatgtatg ggtcacttgc
     3661 ccgaggcggc ttcgatctcg gatttgacct gctcggcggt catcacacgg ccgaacattt
     3721 ccgcccagag atcaaacatg gcggtgttcc agtcgttcgc cgtgcggccg cagtggtcgt
     3781 aggagctgat gccgtcggcc acggcgacaa cgttgtagtc cttatgatag gcctcgcgga
     3841 tcgtgctgtc cacgcaccag ttcgtcaccg tgccgcagat gataagcgtg cggatgcgct
     3901 ttttcttcag ccacgtttcg agctccgtgt gggagaagca ggagtaggtg tacttcggaa
     3961 atacggtgtc gccttcgacg ggcgcaagcg ccggatcgat ctggcactcc ggggagccgg
     4021 gcttgaactt gacgtcgccg tcctcggtga gaaggccgcg ctgcttattg cggaaccagt
     4081 aatgatcgtt gtggaaatcc acatcctgcg cgaagatgga cataaacacg ggaatgccct
     4141 tttcgttgca gaactttttg agatcggcga tggtgggcag ctgctcggcg gactgcggcg
     4201 tgtagagcgg accgtcgggc tgggtgaagc cggtctgcat atcaatgatc agcagggcgg
     4261 tctcttccgg gtggatcgtg aaaggagcgt cccactccca atatctggcc atagtatttt
     4321 tcctccattt tgtatgggtt tacttaaatc aaataaggac gtgcgttcac tcctgctgca
     4381 gcaggttttc gaggatcgtt tcgcacgggt aaaagtcaaa gttgtagttg atcgcgagaa
     4441 gctgcattcc ctgccggtgc tccgcctcgg tcgtggagtc tacgccatcg tccgcaacag
     4501 cgacgcggaa gccgcggtaa aacgcttcga tcgccgtctg cgccacgcac acgtccgagc
     4561 gccagcccgt catgatgaca gtgttgatct gcatctggcg gagctttttt tcgagcgtcg
     4621 tgttgacgaa gccgttgaac cggtttttgt agacaacgat gtcagccgga tcgggcttta
     4681 cctcgtcgat gatctgcacg ccataggagc cggccatcat atgattgttc cagagctgca
     4741 gctccggatc gttcttataa tgccggtcgc agacgtaaat gaccggaacg cccatctgcc
     4801 gcgcgcgggc aaagagcttt ttgagctgcg gaagaatgtg atggatgcgc ccgcaggcaa
     4861 gcgtgccgcc ttcgagaatg tcgttctgaa gatcgatcgc gacgatggcc ggacggcaca
     4921 catccggcgc ggcgggaagc gccgcggcat cgatatcctc cgacaggaga gactgcaaga
     4981 gcatctgcgc gctcttatac tcttccagta tgcggtccac ctccgcctgc tggcggatca
     5041 tggctttctg cacttcttcg gtggatgcgt cgagcagcgc ggcgatttcc gtcaggggga
     5101 atttcatctc gcggaaataa cggatctgct gcagacgcgc gatgtccgat tctgaataca
     5161 tccggtatcc ggcatcggtg cgctccggct tcggcagaag gccgcggctt tcgtagtgcc
     5221 ggatagcttt cacgctcagt ccggtgaggg aagccgcttt ttttatcgtt atcatgcagc
     5281 gcgctccttt ccgtgccggt gggatatttc aaacgggagc atcccgaagg atgctcccgc
     5341 gggaggcatg gcccgccgtg gggccaatgg atttactgct tcttgccgac cttgttgaag
     5401 atgaccttca cgaggaaagc cacgacgacg cagttaatgg agggcaggag aacggggatg
     5461 aaggtgacag caacgccgac gatccaggac acgaagccac cgacatcagc gcggggcagg
     5521 gaatccagat cggtgtagcc gtggttgtac ttggtgagga agtcagcgaa cacgatgccg
     5581 acgatgggag gcacggtgct ggagaggaag ttgatgtagc tgccgaacag gttgtagaaa
     5641 ccaacgcagc cgaggatggt gccgacaaga ccgaacacga gggtcagctt gttgcgggga
     5701 accttgatga cggagctgag gctcatagag ccggagtaga cgcagccctg agcggtggac
     5761 cagatgttga ggaccatgac gatgaacgcc ggggccagaa gaccctgcag cgcgagaacg
     5821 cccatgatgt cgggatcgcc gtaaacaacg gagccgatgg cgccgaagaa caccatgaag
     5881 gagttaccga ccatgatcgt gatgatcata acaatgatgg aggtctttgc gttcttggca
     5941 aagcgcatga tgtcgggcgt gaacataacg gagccgacaa cgtaggagcc gacgcccaga
     6001 gcaacggcct tcgcgaaggt gatggagtct tccttgacaa gagcattgat gccggcaacg
     6061 ccgccgacgt cgttgaacgc gcggatgatg gagatgaagc cgaagatcag gacgatgggc
     6121 acggcgatgt tggagagcat gttcatccac ttggtgccct tggaggcggt gatggtcatg
     6181 ccgataccgg cgaggatgca gatgaggatc gtgggcatcc aggagaagag acggtgcgcg
     6241 gagtcgccga tgaggtaagc gtcgatggag aaccagccga tggtggtgat agccacgatc
     6301 agcaccggca cccatgtgcc gacctggccg aagctgtagc gggccatgag accgaagttc
     6361 aggccgctct tagcggcgat gacgcccatg atgcagctca gaacggtgag gatggcgctg
     6421 gataccagaa cggcccagac catgctgttg aaggagaggc tgacgccgat gacgctgcca
     6481 gcctgcatgc tcgtaacgac gaacacgtag ccaagggaaa tgatgagcac ggaaaggaag
     6541 gatttgcgag cggtcgaagg aacggcagtc ttactgtatt cgtccgcggt ggtctctaca
     6601 gtaacattct ttttttcttc cattgcgaag gtaacccctt tcgtttcgtt cagctgtctt
     6661 tcagcgctga aagacgcggg cccgggttcc cgttaatcaa cgcttaccga ctgtctgatt
     6721 gcatcatagg cgagatgtta acaaaatgca aacacgaaac cttcccctta gggtatactt
     6781 taccataaac ctcggagcgc cgccatagac aaaagataga aaaagggccc ggtttttccg
     6841 tctgcggctt gcgtttttcc ggaaattctc tataatataa gggctatgta tattttttta
     6901 cgaaacaatg attatcgtta cgccgccgag cagatgctgc tcatgctctt ccccgaagag
     6961 cggccggtgt acccaagttc cgatctcgga ctccgggagg agaatgctgt cgagctggag
     7021 ctgaagccag gcaagacgta cacaacggct gtgtgccggc tccggtacga ccggcgcacc
     7081 gcgcaagatc atgtccgcgc ccgcaccgac ggcatccgcg ccggggagga gagagcccgc
     7141 atcgagcagc gcatcttaaa gctcgctttt taccgcgccg cgctggacgt cggcgtgccg
     7201 aagccggagt ggggctgcct taccggtgtg cgtccggcaa aattcctcgc ggggctcatg
     7261 cagaaggacg gcctgaccga gaccgccgcc gtgcggatgc tgaccgaaac gttcggtgtg
     7321 tcgaaagagc gtgcgtctct cgctctcgcc gccgagcgcg ccgcggcacg cgccaaagcg
     7381 gtgctcgcgc cgcaggacgt gtgcctgtac atcggcatcc cgttctgccc gacgcgctgc
     7441 gcctattgca gctttgtgag cgtggacgcg ccgaagattt tgaagagcat cccggattat
     7501 ctcaacgcgc tcgagcagga gatgcgctcg actgccgcgg cggtaaaggc tgccgggcgg
     7561 cgcgttgttg ctgtatacat cggcggcggc acgcccacga cgctctcggc gccggagctc
     7621 gaccggctgc ttgcggacac gaaggcgtgc tttgatcttt ccgcctgccg ggagttcacc
     7681 gtggaggccg gacggcccga caccgtgacg gaggaaaagc tcgcggtgct cgtttcgcac
     7741 ggcgtaaacc gcgtgagcgt caacccgcag acgatgtcgg acgcggtact tgaggagatc
     7801 gggcggcacc acaccgctgc gcaggtgcgc gaggcggtgg cgatggtgaa aaagttcccg
     7861 cagctcgcct tcaacatgga tctcatcgcg ggtctccccg gcgatacgcc ggagagcttt
     7921 tccgacaccg tgcgcgaggt gatcgcgctg ggcgcggaga atatcacggt gcacacgctc
     7981 gcgctcaaaa agggctcgcg attcctcacc gagggtacgg cgctgccgga cgggaaggcc
     8041 gtcggcgaaa tgctcggcga tgcgcgtgaa aagctcgaag cggcggggta cgcgccgtat
     8101 tatttttacc ggcagaagtt catgtccggc agctttgaaa acgtcggctg gacgaagccg
     8161 ggatacgaaa atctctacaa catatgcatt atggaggagc tctgcggcat tctcgccgtg
     8221 ggcgcgggcg gctctacgaa gctcacggcg cccggcggcg tcgtaaagcg tctcatggcg
     8281 ccgaagtatc cgcgcgaata catggaccgc atcgcgcgca cgtgcgccga aaaggacgag
     8341 atcgtccgct tttataattc cgaaccgaaa gggacaaact gaaaatggcc tactatcttt
     8401 ccgagatcaa tgcccggtgc cgcgccgacg ccgccgggtt catcgccgag tgcgacagca
     8461 tttacgccga ccggctggag ctcgccgccg accgcatcat ggagaacatg gaaaaaagcc
     8521 cgatcgtcct gctctccggg ccctccggca gcggcaagac gacgaccgcg ctgaagatct
     8581 gcgaggtgct tgagcggcgc ggcgtgcgca cgcacagcat ctcgatggac aactatttca
     8641 agagcgtctc cccggagacg acgccgcgca cgcccgaggg aaagtacgat ctcgaatcgc
     8701 ccctgtgcat ggatatggag cttttgaacg agcacttctc cgtcctttcc gaaggcgggc
     8761 gcatccttgt gccgcgctac gactttgtgc accagtgccg cgatctggag ccgagcgctt
     8821 cgctgcgtct gaagaaaaac gaggtcgccg tgttcgaggg catccacgcg ctcaacgact
     8881 cgatcacgct cgtccacccc gaggcgttca agctctatat cagcgcgcgc agcaacattt
     8941 tggacgatga cggcgcggtc gtattcaagg gcacatggat gcgccttatg cgccgcaccg
     9001 tgcgcgacta tcttttccgc ggcaccgccg cgcaggagac gcttgcgcac tgggccaaca
     9061 tccgccgcgg cgagaagctg tatatctcgc ccttcaagga caaggcgaat ctccagttcg
     9121 actcgtcgtt cgcctacgag gtgccggtgc tcaacaacac cgccaccggc cttttcaacg
     9181 ccatgccctc cgacacgccg cgctacagcg agctgcagag catcctcccg gcgttcgagc
     9241 gctttgagga cgtctcgccg gagcttcttg caaaggactc gctgctgcgt gaattcatcg
     9301 gcggcggaaa gtattcgtac tgaccgtttc cccggcccgc gccggaacac aaaagagaga
     9361 aaggacgcat accgcccatg ccgcagttcc gcgatccgaa cgaaaaacgg gattttatgc
     9421 tcaatacgcc cattcccagg ctcgtgccga aaatggcgct gccgtccatc gcgggaatgc
     9481 tcgtcacgag cgcctataac cttgccgaca cgctcttcgt aagtcagctc ggcacggacg
     9541 ccacgggcgc ggtcggtgtg aacggctcga tcgatctcat cattatgatg gcgggctcgc
     9601 tcctcgccgt gggcgcggcg agccttacgt cgcgtctgct cggcgcgaag caggacgagc
     9661 gcgcccgcga ggtgctctcc acatgcttct tcctcgcgct cgcgcttggg acgcttgtac
     9721 tgatcctcgg ctttacgtat cagaataaga tcctgctcct gctcggcgcg aacgaggaga
     9781 tcctgccgta ttcggagcag tactgccggt atatcctgct tgccgcgccg tttatggcga
     9841 caagctttgt gctcaaccaa tgtctgcgcg ccgagggcag cgctgttttc tccatgatcg
     9901 gcatggtggc gggcgcggtg ctcaatgtgg ggctcgatcc gctcttcatc ttcacgttcg
     9961 gctggggcgt cgcgggcgcg agcgctgcaa cggccatatc caaggtcgtc agctggtgca
    10021 tccttatgac gccgtatttc cgcaaaaaga cgctgctcac gatccgcttc cggcagttcc
    10081 gcccgcgctg gagcgatgcc aaagaggtct gcgccatggg cagcgcgagc tttttccgca
    10141 acggtctcgg cacgctcgcc gggatactgc tcaaccgcat cgccgtgggc tacggcacga
    10201 gcgcgctcgc ggcgatcaat gtggcgaacc gcatcaccat gtttatgacg agcgcctgtc
    10261 tcggcttcgg ccagggcttc cagcccgtgg cgggcttctc gtggggtgcg aagcggtttg
    10321 accgcgtgcg ggaatcgttc cggttttcca tgatcgcatg cgtcgccggc atttccgctg
    10381 tggcactcgt tgtcgggatc ttcgcccggc ccatcctgct gctttttacg gaggacgatg
    10441 ccgagcttgt gcgcatcggc gtgttctctc tgcgcatcca gtgccttgcc atgccgttcc
    10501 acggcttcgg catcatcgtg aatatgctct gctccggcat cgggcgggcg ctgccgtcgc
    10561 tgctgctggg gctggcgcgg cagggtatct gcttcttccc gatattgcct cttatggtcc
    10621 ggctctgggg cgtgtggggc atcgcgtcgg tgcagggcgc ggcggacatg ctcgtgatgc
    10681 tgctcgccgt gccgatcgcc gtgcgcgtct cgcgcgacat tcggcggcgg gaggccgagc
    10741 aggggggaga aatatccccg gtgtaaggtt tttcgcgcga atggatgttt atgcttgact
    10801 ttttcctttg gcgcggtata ctttttagca gaaactcaat ggtagaggcg cgatcaacat
    10861 cagtacgctc ttgtcatccg gcagacaccg gggagcggaa aggggcgatc gccgaagggt
    10921 ctcaccgctg tctggcggtg gatgcctggg acacggaaga acattccgcg gactgtcact
    10981 tcggtggaga gctgtcattg ctgcgaattt agttttttta ttactgattt tgtatgcaca
    11041 gccgtgatcc ttccaccggg aatcacggct ttttgcatgc gagggaggtt tttctcatgt
    11101 tttacgctac catctgggcg ctgctgccgc cggtcgtcgc catcgctctg gcgctgatca
    11161 ccaaggaggt ctattcctcg ctcttcctcg gcatcgtcgt gggcgcggcg ctcgtcgccg
    11221 ggttcgcgcc ggtaaaaacg ctggacgtta tcatcaacga cggcttcatc gccggcgtgg
    11281 cggacgcctg gaacgtcggc attttcctgt tcctcatcct gctcggcatt ctcgttgcgc
    11341 ttatgaaccg cacgggcggc tcggcggcgt tcgggcgctg ggcggaaaag cacgtgcaca
    11401 cccgcgccgg cgcgatgctc gctacggtcc ttctcggcgt actgatcttc gtggacgact
    11461 attttaactg cctcaccgtc ggcagcgtca tgcgtcccgt gacggacggc caccgcatct
    11521 cccgcgccaa gctcgcctat ctcatcgacg cgaccgccgc gcccgtgtgc atgatcgcgc
    11581 cgatctcctc gtgggcggcg gcggtctccg gcatcgtgga cgagagcgtc atgtccggca
    11641 cggagctgtt cgtccgtgcc atcccctgga actactacag cctctttacg cttgttttcg
    11701 ttgtcggcct ctcgctcatg cagtttgact atggcccgat gaagctgcac gaaatgaacg
    11761 cgcggctcaa cggcgatctc ttcacctccg gcgagcggcg cagcgatgag gcggatatcg
    11821 tcacttcgcc gaagggacgg gtcgtcgatc ttgtgctgcc ggtgctcgtg ctcattgcct
    11881 gctgcgtcac cggcatgctg tatgtcggcg gattcttctc cggcacgtcg tttgccgcgg
    11941 ccttcgccgg aacagacgcc tccgtcggac tgccctgggg cacgctcatc gcgctcgtgt
    12001 tcaccgtgat ctactacatc tgccgcggtc ttttgagctt caaggaagcc atggaatgca
    12061 ccaccaaggg cttcatcgcc atggttccgg cactgctcat cctcacattc gccgtaacgc
    12121 tcaagaacat gaccggcatg ctcggcgcgg cggaatacgt gttcggcgtg atgcagggcg
    12181 cgtcccggag cctttataac ctgctcccgg cgatcatatt ccttgtcggc tgcggccttg
    12241 cgttctccac cggcacgagc tggggtacgt tcggcatcct gatcccgatc gtgacggacg
    12301 tattccccgc ggacagccag cttctcatca tcggcgtttc cgcgtgcctt gcgggcgccg
    12361 tcatgggcga ccactgctcg ccgatctccg atacgacgat catggcctcc gccggtgcgc
    12421 agtgcgacca tctcaaccat gtttccacgc agatccccta cgttctcacc gtcgccgcga
    12481 tctcgttcgt gagctacgtc atcgccggat tcgtgcagaa cgtgtacatc tgcctcgcca
    12541 tcggcgtcgc gctcacggtc gccacgctct ttgtgctgcg cgccgcaacg tcccgccgcg
    12601 agacagtaaa agcttgaaca tccaataacg agcctcgcag agtttgcgcc cgcaggcgca
    12661 aaaagaattc aaaacatttt tgccggcgac acgcgcgtcg acaaaaatac ttttcggctc
    12721 gcaaacgtgc gagcgaagcg a
//
LOCUS       DAWAAE010000050        12663 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2233_length_12663_cov_3.278871, whole genome shotgun sequence.
ACCESSION   DAWAAE010000050 DAWAAE010000000
VERSION     DAWAAE010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12663)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_12663_cov_3.278871"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6882..6891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2579 a         3695 c         3609 g         2770 t
ORIGIN      
        1 cttattcgag cgagagagtg cagtcatgcc ccgtgtcggt gcgggcgatg agcgcggtga
       61 gcttttcaaa gagcgtttcg ccctcgcccc ggcgcgcttc ggcgagcagc gcctccatgc
      121 gcgggcggtt attcgggtgg cagcggcgca ggagcttttc atagtagatc accgtggcgc
      181 cgtagccgaa gtcctcgcgg ctcttttcgg agttgagcca gcggatgtgc agccgcgcgc
      241 ccgccggaat cttgccgatg ccgccgccga gcaggtcgtt gagcgcgtca aggctgtgca
      301 cagcctcgtc cggtcctacg tcaaagagac ggttgaactc gcggaacagg ccgtccatcg
      361 tggagaagtt cgccccgtcg atcacaaggg tgcggcggcg gccgtcgctc ggttcggccg
      421 ctgcagctgc cgcggtactc cggcctgcgc ccttgccgcc ggatttcttc gctttgagcc
      481 tatcgatata gtcccggaag tcggcggtat agaagctctc cggaatgcgg ttggagatcg
      541 cgtatccggc aaagccgccg atcgagccgg tgatcacgcc gccgagcacg tcggtagcgt
      601 agtgcacgct gagataaatg cgtgaaacgc ccatggcgag cgcgaacagc agcgccgtcc
      661 agctgacctt cttgttgccg cggaggaaca ccgcaagcga ggccgccatt gccgcggtca
      721 tatggcccga ggggaagctc ttgtcgcttt cggtatgctt gccgagcatt tcccaatact
      781 gctggaaaat gctgccgtcg tgcgtatacg ggcggggccg ggcgacgagc ggtttaaagc
      841 agacgtttgt taccaaagcg ccgatggcaa gcgcgagcag catcgttgtg ccgtaccgcc
      901 gcgttttgcg gaaaagcatc agcgcgatcg agagcaggat gaggaagata ccgcccttgc
      961 cgaggagcga aatgaacgag aggaacgggg tgaagaacca cccggcggcg acgtagagct
     1021 gatggatcgc ggttgcgacc gcttcgtcaa agcccgcgaa ggccgtattc agccacagcg
     1081 ccagagccgt gagttgcata agccggactc ctttttcttt taaataatcg catggatatc
     1141 tttggtatta taaccgagcc gcgccggaat tgcaagtcag ccgacgacac gcccaaatac
     1201 gcgcagctcg tccccctccg ccacacggat gggggcatac cgcgggttga gcggcacaag
     1261 ctccacgcgc cccggaacgc gctcgaagcg cttgcagtac cccgcgccgt tatagaggaa
     1321 cacgccgatc tcgccggagc ggagcgtttc ctggcgatgc acccagacga tctgcccgtg
     1381 aacaaagcgc ggctccatgc tgtccccggc aatgcgcacg ccgaagtccg cgttcggcga
     1441 tacctcgtcg ccgacttcga cggtgtcgta atccgtcccg tcgagcagct cgcccgtacc
     1501 ggcggatacc gccagccggt agagcggcag cgtccgcacg gcgcgccgct cctccgcggg
     1561 cgcaaacgcc gcaacgacat cccggatatg atagagacgg cagagctcta caagctgacg
     1621 ggcgctcggc agcgttgtgc cgcgctccca cttgctcact gcctgcgtcc gcaccgggaa
     1681 gcccgcctcg gtgatatatt ttgccacctc cgtctggttg tatcccgcgg cgcgccgcgc
     1741 cgacgccaga cgcgcgccga agctgttgtc gtccatgggc gttctccttc ctgtgggtct
     1801 gagatcttct atctacaata tatagggtac tgccaaaaaa cgcaagcctt tttctcttat
     1861 aaagcgaaaa attaccgctt gatttcgctt ataaagcgaa ttataatgca attaatcgct
     1921 tgtaaggaga atttttcggt atggacgcgc tgaatatgcc tctggagatg atcgccgtat
     1981 gctcgccgga cggggagctt tccccggcgc ggttccggct ggaaacggaa agcggcgagc
     2041 gcgtcacggt gcgcatccgt cagatccgcc gccgggagga gatccggctc atgggcgtgg
     2101 aatcgatccg gttcgactgt ctggcgctgc tgggcggaca gctgcggcgc tttgagctgc
     2161 gctacggcgt gcgcgcgcac cgctggacgc tctggcagtt tctcgaccgg gatgttcccg
     2221 cgagccgata gataatctac acatgtgaca aattcaaaaa tctgttgcag ctttttagcg
     2281 gcgttccggg ttgcaaagcg gaaaaattgc tatataatgt ggtatattgt aaattcttcc
     2341 gttatggggt ggttctcatt cgctgggacg ttttcttcct gctctgggca gggatgatgg
     2401 tttttcatct ggtcacatat ttcggctcgc agtatcttga gggtgagccg cacaccgtgg
     2461 gcggcgcgct cgacgagcgc attccgttcc atcccggatg gatctatatt tacagctcct
     2521 ggttcgtgct gctgctcggc gtgccggcgc tgctcaacat cgttgcgccg gagctctgtc
     2581 tcaaatacat gctcgcgcac agcattgacc agcttttgag caacatcatt tatctgatct
     2641 acccgacgac gtttgaacgc cccgtaccgc cgaagaaggg gctgaccggc ttcgtcatcg
     2701 gtacggtgta cggggcaaac caccgctttc tcaactgcgc gccgagcgtg cactgctcgg
     2761 tgtcgttcct cttcgcattt gccgcgctcg ccgcgcccat gctgccgctg tggctgcggc
     2821 tggtgatcgt tgtgtggtcg cttgcgatcg tcgcatcgac gatgctcgtc aagcagcaca
     2881 tggtcatcga cgccgtgacc gcgctgccgg ccgccgcagt ctgctggctg ctcgcagggc
     2941 tggtgaactt ctcttctctt gccgcaaagt tttcttaaat aaaatcgtcc gccgcattcc
     3001 gatggaatgc ggcggatatt tttcgttttt cctttatttc agggcgcggc gcagcgcgtc
     3061 ggcgacggtc tgcaccgtct cacgcgtgag atacggatgc atcgggagac tcagcaccgt
     3121 ggcgcacagc tcctccgtca ccgggcagag aatgtactgc ttcaagccgt cgaacgcctg
     3181 ctgggtgtgc atcggcttcg ggtaatacac catggtgggg atgccctgcg ctttaagctc
     3241 cgattgcacg cggtcgcgct cctcccggct gcgcagccgg agcgtatact gcgcccagct
     3301 cgaataccag ccctccggaa cgaccggcag gatcgccggg ataccggcga gcgcttcgtt
     3361 gtacatggcc gctgccgcgt tcacgtccgc gagctcgtac ttttcaaggg cgtccatctt
     3421 cacgagcagg atcgccgcct ggatcgtgtc gagacgggag ttcatgccga tgcggacgtt
     3481 gtcgtattta aaagagccct tgccgtggac gcagagggag cggatcatgg ccgccgtttc
     3541 gtcggagtcg gtgaacaccg caccgccgtc gccgtagcag ccgaggggct tcgcggggaa
     3601 gaaggacgtg catgccgcgt cgccgaaagc gccgttgcgc ttgccgtgca gcatgccgcc
     3661 gaagccctgc gccgcgtcct ccaggaagag caggccgtgc tttttgcaca cggcggcgac
     3721 cgcctcatgg tctgcgggca ggccgaagag atcgacgtcc accacggcct tcggcgtgaa
     3781 ctttccttcc gcaataacgg cttcgatggc cgcctcaaga cttttcgcgt ccgcgttgaa
     3841 cgtatcggcg cagacatcat agaataccgg cgtgccgccc acggaggaaa cgatctcgcc
     3901 cgaggcgaag aacgtgaagt ccggcacgaa cactgcgtcg ccggggccga cgccccacgc
     3961 catcatgcac agctccagcg cgtcggtgcc gttgccgcag gtgatgcagt gcttccgccc
     4021 gacgtaggcg gcaagacgct cctcaagcgt tttcacctgc tcgccgctga tgaaatgcgc
     4081 gccggacgcc acggcgatca tggccgcgtc catctccggc ttcaaaacct catactgctt
     4141 ttgcagatca cgaaattgca ttagtccttt acctccgtaa gtccgttttc gccctcgcgg
     4201 tatttccggc cgcatttggg gcagttcagt ccatttttca aaagctcgcc gcattcacac
     4261 gcccagccgc agcgccgcgc cggaacgccg agcatcagcg cgtggtccgg cacattgtcc
     4321 gtaacgaccg cgcctgcgcc gatgagcgcc cagcgcccga cggtgtgacc gcagacgatc
     4381 gtggcgtttg cgccgatgga cgcaccctcg cggatgagcg tctttttata acccgccgcg
     4441 cccttgggat acttcgcgcg cggcgtgaga tcgttcgtaa atacgcagct cgggccgcag
     4501 aacacatagt cctccagctc cacgccctcg tacaccgaga cgttgttctg gagcttgcag
     4561 ccatcaccga tgcggacatt attcgatacg ttcacattct ggccgagcga gcaatgtctg
     4621 ccaatgaaag cgcccttctg gatgtgacag aaatgccaga tgcgcgtccc ttcgccgatc
     4681 gtgacaccct cgtccaccac gctggacgga tgtacatagt attcgctcac cggtcaaacc
     4741 tcccaacata gtccatcgtc gagccgcgag agagcgggaa gcgcacgggc tttccgctcg
     4801 cggcggattc gtatatgccg agcacgagtt cgagcgcctt tcgcccctcg ataccggagc
     4861 agaccggctc gcggtcgtgt tcgatcgcgt cgatcatgtc gcggtagagc ggcgtgtggc
     4921 ccgtacccat gtccgcaacg gcgtattttt cgatcgcccc cggaagggaa tccgtgccgt
     4981 tttcaaactg ccagcgttcg acctgatcga gatacttgcc ggatacggac accgtgccgg
     5041 tctcgccgaa cacggcaagc gcatcgctga ggttattggg atatacgttc accgtgccct
     5101 ccacggtcgc ataggcgcca ttcttaaagc gaatgagcgc gagacccata tcctcggcct
     5161 cgatgtacgg atgcgtcaga cggtcggtgt aggcgaatac ctcgtccgcg tcgccgaaca
     5221 tccattgcag aaggtcggta tagtggatgc actggttcat aagcgtgccg ccgtcgttct
     5281 tccacgtgcc gcgccatttt ccggcgtcaa agtacgcttt gttgcggttg cggcggatct
     5341 gcgccgcgcc gtgcagaagg cggccgaagc tgccgtccgc gacttccgcg tgcagcgcgc
     5401 gcattgacgg gcagaaccgg agctgatggt tcacacagag cttcacgccg tttttcgcgg
     5461 ccgtttcgat catcgcgtcg gcgtcctcga gcgagagcgc gaccggcttt tccacgatca
     5521 cgttcgtccc ggctttgaga caatcgcaga cgatctgccg cttgatgccg ctgccgagag
     5581 cgaccgccgt gagagagggg cgctgctcgg agagcatggc gcggtagtcg gcgtagagct
     5641 tcacgccgga gcgcttctcc tccgggatca gcgcgagcgc cttttctcgg ttttcctcca
     5701 cgagatcgca gagcgcgacg atctccagcc cggtttccag cgcggcgcgg atgtggttgg
     5761 cgcagatcat cccgcagccg atgagagcat atttcatagc tttgtctccc gcagaatatc
     5821 gcagattttt tccgccgcgt ggccgtcgcc gaacggctgg taggccgggt cgatgtgctg
     5881 ctcggcggag agctttttca gaatatcgtt tttgtccggc cgggcgagct gattgcggtt
     5941 atcgaccatt gtctccggcc acaccacatg atccataacg agcaggcact gcacaccggc
     6001 atagaatgct tcgcactgca atccgccgga gtcggtaacg atttttttcg catgctgcgt
     6061 gagatgcacc gaatcggaat acccaactgg ctgcgtaaga atgatgtttt taaagccgtg
     6121 ccgctcgcgc agacgcagcg cacgggcatg gttgcgcgga tgcactgcgt agatcgtcgg
     6181 cgcatcaagg ctgtccatgg cggagaatat ctcagtgagc gcttcgtcgc tgccggtgtt
     6241 ttcctgccgg tggcaggtca tgaagtaata ctccttcggc acgctcacgc ggttgccgtc
     6301 aaagtccaga atgttggggt gctcccacgg ctggttggca aaatgcagaa acgaatcgta
     6361 catgatgttg cccacgaggt gcgccttggc ggcgagattt tccttccgga gattttccat
     6421 gcaggactcc gtcgccgcga aaagaaggcg ggagcaatgg tcggtgcaga ttcgattgat
     6481 ctcttccgga ttatcgttcg tgccgagacg gttgcccgct tccacatgca gcacggggat
     6541 catcagcttc accgccgcga gcgccgcggc cagcgtggag ttcgtgtcgc cgtacacaag
     6601 aagcgcatcc ggcttttcgg cgagcaggac cttttcgatc tcgatgagca tgcgtccggt
     6661 catttcgccg tgcgttccgc cggctatgcc aagattatag tccggccggg ggattcccat
     6721 ctcctgaaag aagatgtccg acatattggc gtcatagtgc tgaccggtgt ggacaagaat
     6781 ctcccggtgt tcgcgccgga gagcacgaga accggccgca gctttgataa actgcggccg
     6841 ggctccgacg acggtaacga tcttcatttt agagcacttc gnnnnnnnnn nagcacctcg
     6901 atgttgccgc gctcggtgac ggccttcatc gcgttcttgg tgtcgaaaac aaattttgcg
     6961 tttttctgca cgaaggcata gtccacgttc gtgtgcgcgg tcgtgaccat gacgaggtcg
     7021 gcctcgcgca gcgcttcttc cgtgagcgcc gggatgccgg tcatgctcat gcccttccag
     7081 cggaaggacg gcacccacgg gtcgtagtac gcgatctctg cgccgcgctt ttgaagctcc
     7141 ttgatgacgt tgatcgccgg gctctcgcgg aagtcggcaa tattctgctt gtaggcgacg
     7201 cccagcacaa ggatcttcgc gccgttcatc gactttttga aggtgttgag gatgcgggcg
     7261 gcgcggtcca cgcagtactc gggcatgcgg tcgttgacca tcatggacga ttcgatcatc
     7321 gaggtgtgga agccgtattc gcgcgccttc cagctcagat aatagggatc gagcgggatg
     7381 cagtggccgc ccagaccggg gccgggatag aacgcctgga agccgtaggg cttgctcttg
     7441 gccgcgtcga tcacttccca gatattgatg cccatccggt cgcagagaat ggcaagctcg
     7501 ttgatgaggc cgatgttcac gttgcggtag gtgttctcga ggatcttctc catctcggcg
     7561 atggcgggag aggagacttt atagaccggc gcttcaagga cggtctcata catggccgcg
     7621 gcgatctcgg tgcacttggg cgtgcagccg ccgacgacct tgggggtgtt cttcgtttta
     7681 tagatcaggt tgcccggatc gacgcgctcg ggcgagaagg caagataaaa gtcctcgccg
     7741 catttgagac cggacttttc gaggatgggc ttgcagagct cctcggtcgt gccgggatac
     7801 gtcgtggatt cgagcacgac gagcatatcc ttatggacgt acggcatgat gctctccgtg
     7861 gaggagcgga cgtagctgat gtccggctgc tggtgagcgt cgagcggcgt cggcacgcag
     7921 atgcacacgc agtcgcactt cgccgcgtcg gcaaagttgg ccgtggcgac gagctgtccg
     7981 gctttcacca gcgcctccag atcctcgttc accacatcgc cgatatagtt tttgccggcg
     8041 ttgaccatgt ccaccttttc cttctgcaca tcaaagccgt agaccttgaa gccggccttc
     8101 gccttttcca ccgcaagcgg caggccgacg tagccaaggc cgataacgcc gagagtagct
     8161 tctctggcaa gcagtttttc cttgagtccc atatgtattt cctcgattcc gttacgaagt
     8221 tgataagcta tccttaggat aatatcacgg ctgatccgtt ttttcaagaa tcaacgtgta
     8281 ttgcgccgaa aaaacgtttt acgggtattg cggtgtacaa cctccggcgg aacaattctg
     8341 cgctccggcc attccgccgc gccgttcggt tcatccgtgg gaaagaagct tcttcctgta
     8401 ttctttcatt cgctccatat ggagcagctc gcccatttcc ttttgagccg ccgcactgtg
     8461 ctcgccgccg ggagcaaatg tcagctcacc gaacacgggt ttgccgccga tatcgtagag
     8521 atccacccgg acaaacggaa acggtgcgct cagttttcgg gcaatgtcca gcatttcatc
     8581 gtaacaagac ggcttcggcg cttcggcata tcgaaattca ttgcaaatat cctgccggtc
     8641 attccagtca acatccttgt atacctctat cggcgtttct ccggaaaatc gatccgaaat
     8701 ccagagaaca taccgcggct ccccgtgaaa acagtaaaac ttataatcgc tggggaatcc
     8761 gcccccgtcc gccgtcatga gatgctcaca aatataatgc tgattttctt tcgccgcaaa
     8821 aaggatgccc tcctggcgga aagtaatccg ttgtttttcc cagcgtcgga gtctttcctt
     8881 tgcctgttcc ggatcaaccg gctctccgtg tgtgcggaat acgtggttac tccagccgct
     8941 ggtcgctttg atcgcgcact gcgccggaag cgagtcccat gggatgtcat tcatgtcgtc
     9001 ccacacaccg tatatcgggt tgagcgtgta ttcacagccc tgctgcctaa caaaatcgcg
     9061 cacacggtat ttatcgtagc attgctgtac caacgggctt ttattgtaaa tatagtactg
     9121 gagccagagc agcttttcat tgaacagctt tggatggctc agatcgatcg ggttttccgg
     9181 tatataatga ccgtatttca cgcgcaggcg aatgtgcagc ttgggaaaca gcggcgccgt
     9241 caggagcaga atagcgtttt ctctcactgc gcggttcgta agcgttttgc ggaaaaacgc
     9301 aaatcccttc gacactttag ataaaaagcc acggatgcgg ttcattgttc gttatccttt
     9361 cttccgtcag aaaccttccg cttgaccgag ccaagcagtt tgtacatctt gtcccacaaa
     9421 aaaagaaacg gcgggctttt gcggaacaga ataaaccaca caccgttcgg aacgataagg
     9481 cagatcgcca ggcggatcaa gcagtggagc acattgtatt ctgaaaacgg cgcgcaaagc
     9541 agcgacacaa gcgcgcaggt agcggcaatc agcgccaaat atccgatata tgaaagatag
     9601 tacggtatcg agcttttttt gaatactttt ttatatatca gatatgggtc aaacgagatc
     9661 atgatcagga tgcttgccgt tgttccaagc agcacacccg taacgcccag accgcaggga
     9721 cccatgagag catacgaaat aaacacattg aagattgcgg agaagaggtg gcggtatttg
     9781 acctcccagt ggaggccgca ggcaacgcgg tatttgtata ccgcaccgtt cagcccatcc
     9841 agcagataat tgataacaat cagagcgact gccgcgtccg gcagaagcca tttggtatcg
     9901 tgcagccata cgccggcaat gaacacattg taaaggtgcc agaagcagat cgccgtgaat
     9961 ccgtacagcc agaaatacgt aaattgcagc acgcgaaaaa ggtgttcctt tttttccgta
    10021 tcctctccgg cgcatacatc gccgatcccg gcggcaatgg agccgaaaag cgtatccatc
    10081 aatcgggaaa tgtatttttt taatgtaaga tagttcccgt agacgcccac ggtgccgacg
    10141 ctgataaatg cggatatgag aatgttatcc gccgaggtca gcgcaatgcc gcacaggttg
    10201 gacacagtca acccggtgac gtttttgaat atatgctttt tggtttcccg gtcaagaggc
    10261 gccgcccccg tttcgcgcag ctctgggtac attttgtccg cctgatactt gagcgcataa
    10321 tttgacgcta tgccaagcag gagccccgat accgtgtatg cataaaacgc cgtcaccggg
    10381 tctttgcgaa gcgcaagaag cagcacgatt tgaagtgcca ttgcggctat ggacgtgccg
    10441 gccgtaacaa gcgcgttgac atagtttttc tgcatacagt acagcaggtt ctgcttatac
    10501 gaaaaaagcc agtaggaagc ggcgttgttc acaaggaaca gaacatagat cactcgaaca
    10561 ttcacgagac ccgtggtttc cttcatgaga tacggaagga acggaagcag cacaagaccg
    10621 gcaagcaaga tgaacgaccc gatgaaaaaa taggcttttt tatagaaaag catcagcgag
    10681 cggatctttt cgctgtcctt atccgcaata ggcttataca gctcatacgt aatggccgcg
    10741 ccgatgccaa gttccgaaaa ggacagcacc gagaggatgc tgctgaaaag gccgtttacg
    10801 ccgagatact cctctcccag cacgtagatg aataccgtgc gggacgcgaa ggagagcagc
    10861 atactgacga gctgtccggc aaagcctgtt atgaaattgc ggaatgcatt gattgttcgc
    10921 atgatccata ttgctgcggg gcagcttcct tataaaagat atttccgata agcgtccaaa
    10981 aggacggtct tttcccgttc ccagcagagc tcccgcttgg cacggagaag gttctgtttg
    11041 caggagttgt aaaactcgcg gtctgtacgc atccgttcca gacactgcgc aaggtcatcc
    11101 acgttttctg gttcgcagat aagccctatg ccgtactcgt ctacaagccg gtgcatctcc
    11161 gggagatcgg aacaaattac cggcgtgaga ctttgcacat tctcaaagaa tttgttggga
    11221 agggtatagt aataacttaa acacaggttt tcgattggca ttataccaac atccatcgcg
    11281 ccggcatatt tccagatctc attgaccggt accggtggga gaaagcggag ccgctccgcg
    11341 gcaccgcact tcgcggcgat ctcttcgagc gtccgccggt attcatcgtc ggaatttccc
    11401 atcaaaatga gcgccgcgga cgggatcttt gccacagcgc gtatacccgt ctcgatgccg
    11461 ttgccggagg aaaggccgcc gtggaacata gcaagaaagg tcacgccctg cggggcaaat
    11521 tcccgcagca cggcctgccg gtgccgttct atttccgctt catccagctc ccagcaatag
    11581 ggtgtcgaac ggataacgac tgggcgctgt ttcaaagcgt ggattttctg aacctcatcg
    11641 gcgatgctgt catttaccat gatggaaaaa gcgcagcgcc gcataagaaa gcgctccttc
    11701 cggcatcgtt tcgcacgatc cttttccttc cgaacgccgc gggcgtaaag atcaaactca
    11761 tgggagtcgt agatcagctt cggacggcgg aggaaaaacc gcgtgactat atacgccacc
    11821 tcaagattcg gaagattatg gcagcttatg acgtccgggt ggttggcatg aagcactcgg
    11881 ccaagccgga tccattcgcc gaagatatgc ttccatttgg agagaataga atgttttccc
    11941 ttcagcggtc tcattttaaa cggcaccagt tcaacaccgg caagctcggc ttccggtacg
    12001 tagtccggtt cataataaat cgccacgacc cggtagcccg cttcctgcgc tgctgccatc
    12061 tccctcttat tccggctggc ggatgaaagc ggaagtcccg gcatcagttt aatataggtt
    12121 ttcaacactg cacctcctca gcggcagatg agccgcttat attccgccgt cagctgttcg
    12181 cagacagcat tctccgagaa attgttccgg caaaaccgcc ggatcgtctc cgggtcgtaa
    12241 tcgtcatacc gctcgatcat agtttccatc gcgtatgcaa gccccgcggg atcttccgtc
    12301 tccacggcaa ggccggtctc gggccgtaca agagttttcc acgcgccggt ttttgtcatg
    12361 ataataggaa gtccgcagcc catggcctcc acaaacacaa tgccgaacgt ctcataccgg
    12421 ctcggcagga caaacgcctg actgtccttc agacggcggg cgcattcctc acgcgagcag
    12481 cttccggcaa actccaccgc gccatcaaga gcaagcgcct ttgcctgcgc acggagattc
    12541 tccatctcct ccccgtcgcc gcagatcgta agcattgcct ccgaatgctt ttttcgcacc
    12601 tcggcaaaag cggccagcag cacatccatt ccctttttgc ggtcgagcag acaaacggaa
    12661 ata
//
LOCUS       DAWAAE010000051        12606 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2249_length_12606_cov_3.072584, whole genome shotgun sequence.
ACCESSION   DAWAAE010000051 DAWAAE010000000
VERSION     DAWAAE010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_12606_cov_3.072584"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3131..3140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2691 a         3638 c         3751 g         2516 t
ORIGIN      
        1 gatcaagttc gtgccggacc gcttcgccaa gacgtatctc aactggatga acagcgtgcg
       61 cgactggtgc atctcccgcc agctctggtg gggccatcag atccccgtgt ggtactgcgg
      121 cgactgcggc catatgaccg tctcccgcac cgacgcctgc gagtgcgagg cgtgccactc
      181 caagaacatc cgccgcgaga gcgacgtgct cgacacctgg ttttccagcg cgctctggcc
      241 gttctccacg ctcgtcacgg gctacgacat catcttcttc tgggtcgcgc gcatgatctt
      301 ctccggctgc gagcatatga agaagatccc gttccacacg gtgctcatcc acggcctgat
      361 ccgcgacccg cagggcaaga aaatgtccaa gtccgccggt aacggcgtcg atcctatcga
      421 ggtcatcaac acctacggcg cggacgcgct ccgcttcaac atcatcaccg gcaactcgcc
      481 cggaaacgac atgcgcttct tcccggagcg ctgcgaggcc atgcgcaact ttgccaacaa
      541 gctctggaac gcttcccgct tcgtgatgat gaatcttacg atcgacaaaa acgagctgcc
      601 cgaaacgctc gagctcgagg acaagtggat cctctcccgg ttcaacgagc tggcccgcga
      661 ggtcggcgag aatctcgata agtacgagct cggcatcgcc gcgcagaaga tctatgactt
      721 catctgggac agcttctgcg actggtacat cgagctcacc aaggcccgtc ttacaggcga
      781 ggacgagacg gcccgcgtgc aggcgcagaa ggtgctgctg tatgtgctca ccgaaacgct
      841 caagctgctc catccgttca tgccgttcat caccgaggag atctatcagg cgctgccgca
      901 cgagggcgac gcgctcatgg tgcagccgta ccccgtttat cgcgaggata tggctttccc
      961 ggcggagagc gcgcgctttg aaaaggtcat ggaggccatc cgcgccatcc gcagccgccg
     1021 cagcgagatg aacgtcccgc cgtccaagcg cccgcacctc tatattgtca ccgaggaacg
     1081 cgaagccttt gagaatggcc gcgattatct ctgccgtctg gcgtatgccg gcgaagtgat
     1141 cgtttcggac gatgtccccg ccgacgccga caaaatggta agcatcgtga cgaagaatgc
     1201 gcgctgcttc ctgcctatga gcgagcttgt cgatcttgac aaggagcgcg agcggctgga
     1261 aaaggagctg gcaaagaacc gcggcttcct cgaaaaccag cgccgcaagc tctcgaatga
     1321 gagcttcgtc tcccgcgctc ccgccaacgt cgtcgccacg gagcgcgagc gtgccgaaaa
     1381 gctcgaagcg cttattgcca atctcgagga atcgctccgt cagctcggct gatgcgcata
     1441 taattagatt gacaaaggtg tcccgaaacg ctacaatgac gcttcgggac attttttaag
     1501 ggttccgaaa actttttgcg gcttttgcac gtctaataaa cagaacagaa aaccggaact
     1561 attcgacctg cgccgcaagg cgcggggaag gagacttcat tatgcgtaaa cttatttcaa
     1621 agcgactctt tgccgttttg atggcggcgc ttttgttcgc cgcgctcggc acgacggcat
     1681 tggcggacga cgcccccgtc atcaataccg acgtacccgc ggcgctcacc gtgatcgaag
     1741 ggcagacgct cacgctcacc gtagaggcaa cgggcgggga gctgtcctac cagtggtacc
     1801 gcgtccaccc ggagggcgat acgcccgtgg agggacagac ctcgaatact taccagatcg
     1861 cttccgtgga cgcctcgatc aacggcgcgg cgttttactg ctttgtgcag aacgtggcgg
     1921 gaaataccca gtcttccgtc tgcaccgtga cggtgctctc caaacccgtt ctcacgcagg
     1981 atgtgagcac caccagccag acgctgaccg agggcgacac catcgcgctc accgccggag
     2041 cgaccggcgc ggcgggtata cagtggtact ataagaaggg cgacgaagcc cccaagcccg
     2101 ttgacggcgc gacgacggcc tctctctccg tcgcggccac gatggagtat aacggcgcgg
     2161 atatctactg ccagttcgta aacgatgccg gcgccgtcac aacgtccttc tgccgcatca
     2221 ccgtgaatgc caagcccgca gcgcccgtaa cgacgaaaaa cccgaccggc gagaccgtca
     2281 ccgagggcgg ccgcgcgatc ttcatcgccc gcgccgacgg cgcgacgagc tacctctggc
     2341 gcatcatcag cgccgacggc acgcaggtct atgactatac cgccgcgcgt accgcgttcc
     2401 cgacactgag catctccggc ggcgacagcg aaacgctcac gctcaccaac atcccctatg
     2461 atatgaacgg ctggaaggtc gcctgcctct tcaagagcga gggcggcgag acgctctccg
     2521 gcacggcgag cgtcaccgtg acgaaaaagg cggcgtctct cagcatcatc acgcagcctg
     2581 tcggcggcac gatggccgta gatgagaacc cgaacttcat tctctccatt caggcaacat
     2641 ccagcgacgg cggcacgatt acctaccagt ggtacagcgc cgcgacgaac agcaccgccg
     2701 ccatgaaggc catccccggc gcgacgaatt ccagctataa gcccgaacag accgagggaa
     2761 cgacgtatta ccgtgtaagc gttcagctca ccggcaacgg tacgacctcc gatccctatt
     2821 actccaacac cgtccctgtg acgttcaccg ccaccaagac gcacacgcac agcttctcga
     2881 gcgtctggga gcataacgat ctctcccact ggcaccagtg cacctgcggc gagcacggcg
     2941 acgaagcgtt ccatacctac acctggacca tactgaaagc ccccaccgcg accgaggacg
     3001 gcgagcagaa gggcgtctgc tccgtctgcg gctatgagac catccagccc atccccgccg
     3061 gtacggacac cagtgaggaa gagacgccgg caaagcccgc caaaaagacg aatacggtgc
     3121 ttctcgtcat nnnnnnnnnn ccgtggcggt catcctcgtc cgcaaggtgc ttcttcagaa
     3181 ggacgaagag gacagcggag acgatgacga tgggaacgaa taattcctat aattcctaag
     3241 gcacaaagga tagcttttcg tgaatcgttt gtacggcgtg cgaaaaagaa gtgccggaaa
     3301 gaataaaagc aaagccctcc gattaactcg gagggcttat ctattttatt gacagctgcg
     3361 cactacatct tcgagaatgt cttccataac ggttgtccaa ttggagcgta ccggctctgt
     3421 ttcggacgag gcgccttcac atagatcgag cagagagccg tgagaagtgc ttctggaaac
     3481 attcataaag cggtaatttc cttcatcgga aaacgtgatc acgaaatctc cgttgtttcc
     3541 ttcaacgatg cgattgaaga attaaccgat cagaccgcgc tttttgttgc cggatggaaa
     3601 ttcactgata agtatacaat ccgtaacatt cagtccgatg aagtaagtaa aggaatccat
     3661 agacatttgc tcgtgcgaga taaagcagcg tatacccgct tgctcggctc ttgagagaat
     3721 gcgattttca aaatcctcct gagaggaaac ctttagaccg tttggaaacc gaaatgtttt
     3781 ccagagatac aacgcgcttc cgttaacagg catagtaatt ctcctttttc tttttacata
     3841 tattgcaagg ccagtttctt tgtataggcg cgatagagaa aatgcgcctc ctcgcgcttg
     3901 ccttgctcaa tcatcgaaac gcagggggcg acgagatccc ggtaaatcgt ttcaaaaatg
     3961 tccgttctgt ccggcctttg ctggatcgac gcaacgatcc gcggagcgac ggcgtaatat
     4021 tccgcaatct ccgcttcgcc ctccggctgg gaacggagat atccgtcacg aaagctgcgc
     4081 agcaccgtca gctcgtggca gtcgtccggc agaccgcgtg cctcggtgca ggctgatgtc
     4141 aggaagcagc cgccggagtc attgcccttg tagatggggc agtcgtcgta atcgtagtta
     4201 cggcagtatt tgtcgtatgt gtcctcgttg acgtcccttc cgctcttccg gcaggcgtaa
     4261 tggtgattgt accagtaaaa tggacagctc atgcgtttcc tcccctttgt ttcgtttctc
     4321 tgaaaagtgg aaatattgcc tctgcaggat gattatactg caatttgcag taaaagtcaa
     4381 tacaacaaaa tgcagtacgc tattctttct cctgtcagga ggtggtagca tgtacttccg
     4441 tttgcgcgat ctgcgggagg accacgatat gaagcagaaa gatctggcgg cgtatctgaa
     4501 ctgctcgcag caggtatata gcaactatga gctcggccag cgtgacgtac cgacggatat
     4561 cctcatccgc ctgtcacagc tctatcatgt gtccgtggat tatatcctcg gtctgaccga
     4621 taacccgaaa agaaaccagt aaaaaaaccc gccctccgat ccgcggatcg gagggcgggt
     4681 ttacgtttgt tggattgttt cttagaacgc cagcagcgcc atggagagga tggcagcggt
     4741 cagcaggtag atcggcaggc cgcgccacgc cttcggaacg tagcgttcgc ggatcttgct
     4801 gcggacggcg gcaaacgccg cgagagcgag ccagaagccg agacccgcgc cgagcgccgt
     4861 gaggatgctc tcaccaaagg agagcgcatc ggcggcgttg ttcaccgcga gaccgagcac
     4921 ggcgctgttg agcgcgatga cggggaagta gacgccgagg tcttttttga aggcggcctt
     4981 tgcaacggca ttcattgtat agacaacgat gagcacgatc gcggcaaaga cgagcgtctg
     5041 cagatagccg agagacagcg tgtccagaac gtaggtctgc acaagaccgg cgatcagcgc
     5101 cgtgagcacc atggcgacgg ttacagacac gccgagcacg gtgatcttct tctcctgcgc
     5161 ggaccagccg agcaggggag ccgcgccgag gaatttctcc agcgcatagt tgttggcgag
     5221 catcgcgccg agcacgatac cgacaatggc tttgaacgtc attactcgtt acctccttcc
     5281 gctgtaccgg cggcgacacc ggcgaagccg ggctcttcct ccgcagcgca cgcggcgggg
     5341 aagagggcgt tgacgatcgc ggcgacgatg gcatacacca ggaagccgcc catgggcttc
     5401 tggagcgaag ccacggcgtg cgccttgagg aaggcgatct cctgtcccca gacggtgccg
     5461 agaccgaata gttcgcgcag cagcgcgaca acggcgatca ccgcgaggaa ctccagcccg
     5521 gtcacaacga cctttccgac acagagaccg gaggcgctgc cggtgctgcg gctgaacacc
     5581 agcaggttga cggcagccac ggcgagatac acgccgagca tcttgtaaac ggacggcagc
     5641 accgcgtgca gcacgagctg cgcagcggag gtgaacagcg ccgccgtgag gatcgtcgcg
     5701 gcgatcaccg cgcgctccgg gagaatgcgg cgcagcagcg ccatcacgag cgcagtgagc
     5761 agcatcacgg cgagaaccgt aatgcccatg gcagcggcac cccggagcgt tccggtcgcg
     5821 cccagcgcgg ggcacgcgcc gagaaacagc acccacatcg ggttaacggt cattttcttc
     5881 tcattcattg ccaagtcctc cgttctcttt cagagtggca aaggcgtcga aaacgtcgtg
     5941 cgtagcggtc ttgacggcgt tcatcgtcat cgttgcgccg gagatgaacg ctacatcgtc
     6001 cgtcatggtc tcggcggtca aaccgacgaa gccttccttg tagctctgcg gatcgagtgt
     6061 gtagttggag aagtagtcct tttccagaat gaactccttg gcgttcatca tcacgatctc
     6121 gccgtcagcg ttgagaacat agtagacggc catcgggatg ttgctgtagc cgtagggacg
     6181 ggaaacgaag gcatagagcg tttcgccgtt ctccgtcacg gtgtaggcgg cggtcacggt
     6241 gttgtatacg ccgtcgagac cgagcaccgc gtaatccgcg tccttggagt agtaagcgct
     6301 gagcttggag atatcgtcgt ccgtgaagag atcgccgttg tcgcgcgcat acgcggcggc
     6361 ggtctcggca agatcggcgt aggaatcggt cacaacgctg ccttcaacgt catagacgca
     6421 gacactgccg gaggtgttcg cgatggcaag cacgcccgtg ccgtccacgt tcgcaataaa
     6481 cgcgaagccc gcgccgttcg gagccttgta agcggcggtc acaccgtcga gaccggtctc
     6541 ggtctcctcg ccctgcatgg cgccttcgct gttcatcatg ccggggaaca cggtcgcgat
     6601 cagctcggtg agaagctgtg cgtcgccctt gacgcccgcg ctgacgagtt cattcgccgt
     6661 gagagcggtg aagccgtcga gaatggcgtc atggaacgcc ttggaggagt acgtcacgcc
     6721 cgcgacgaga ctcacgtccg cgagagtgga atcctgtccg atataggtct gcggatacgt
     6781 atccacaccc atgtccttcg tgtcggggta ggatagcacc tgcgtgccga cgatcttacc
     6841 ctcggtatcc acggccatgg agatctcgat cggatcgtgg gtgtagccct gcgtggtgga
     6901 gagaaggatc gcgtagccga gaccggaggt ctcggtatag atgctctgca cggtttccgg
     6961 aacgtcggta agttcgcttc cggcggaacc gtcgctgctg tagagaagat caaagccctg
     7021 cgcgccgggg aaaatatcca tgagcggccc gagcgcctgg gaagcctcgc tcagcccgat
     7081 gcgcggcccg gcccagacat tgagaccggt gagcagcaga gcgaacacga gaaagagaac
     7141 gatcagcgtc agagccgagc ggagaaagct gcctttattt tgcatttttc ggccctccca
     7201 atccgagcgg cgtagccgcc gtccattttt cgatcatcgg agcaagaatg ttcatcacga
     7261 ggatggagag cgacacgccc tccggatacg cgcagtattc gcggatgacg aacgtcagaa
     7321 tgccgcagcc gagcgcgaag aacgccttgc ccttgttgtt gatcggcgag gtctggtagt
     7381 cggtcgccat gaagatcgcg ccgaggaaca gaccgcccgc catgatctca tagagcgcgg
     7441 taacggcgct gccggtcgtg acgaagtaga gcacgaacac gctgccgacg aagatcgccg
     7501 ggacatacca gcggatcacg cggcggatga cgagatacgc aaagccgatg agcagcgcga
     7561 taacgcacgt ttcgcctact gcgccgccgc gcacgccgag gaacatatcc agcagggagg
     7621 gaagaccctc gccgccggtg gagagaactt cgagcggggt ggcgctcgtt acgagatcaa
     7681 cgcccttcgg catcgcgccg cccatgacct gcgtgaacga gacgagcatg aacacgcgcg
     7741 cggtgatggc ggggttcgca aagttgtggc cgagaccgcc gaacagacac ttgacgaaca
     7801 cgatggcgaa cacgctgccg agtacacact gccagaccgg cacgtttgtg ctgagattga
     7861 gaccaagcag cagacccgtc acaacggcgc tgagatcgcc gatggactgc ttgcggcggg
     7921 tgattgcgtt gaagagaaat tcggaaacga cggcggcgat cacgcacgag aggatcggca
     7981 caagagccgg ggcgccgaaa atcacgatcg tggcagccgt ggcgggcagc agagcgatga
     8041 tcacgtcaag catgatgcgc tgcgtgctcg cgccgcagtg ggtaaacggg gaagaggggg
     8101 tttcaaaccg cttatccatc actttttccc tccttccgca tgatcggctt tgtatttttt
     8161 gagatatttc tttgccgcgc ggttgttctc cgcgagcggg cggtgcgccg ggcatacgta
     8221 gctgcaactg ccgcactcga tgcagaagtt cacatggtcg cggtcaagga gaagcgcgcg
     8281 cttatcgccg ttctcttcct caagatcgtc cgagaacgcg gtggggttga gattcatcgg
     8341 gcagtgggac acacaccggc cgcagtggat gcacggcgtc ggctcgtgga taacgctgtc
     8401 cgcttcggag aagaccagca cgctgccggt cgtcttgagt accgggtagt cgagcgaggg
     8461 gacggcgtga cccatcatcg gcccaccgtt cacaacacgg cccggcttgc atttgagccc
     8521 gccggcggcc tcgaccagat agcggtacgg cgtgccgatc gggacgagca ggttcttcgg
     8581 ctccttgatg gccgagccgt caacggtgac gacgcggtcg acgagcggca tgccggtgtc
     8641 catgaacttt gcgaccttgg cgagcgtcga gacgttgatg atgataacgc cgagcgttgc
     8701 aaaacgctga ccggcgtaaa cgaccttgcc gagcgcgtta taaaggatca tctgcttcgc
     8761 gccctgcgga taggagctcg gcagctcaac gatgctcatc gtggggtcgt cggcaaaaag
     8821 ctccttcatc gcggcaatgg cctgcggctt gttgttttca atggcgaaaa caaagttgtc
     8881 gggcttgacg tatttcttga ggagctcgat accgcggcgg atatcctcgc cgtggtcgag
     8941 catcatgcgg ttgtcgctcg tgacgtacgg ctcgcactcc gcaccgttga tgagcatggt
     9001 cttgacgttg tggcgcagcg ccgcgtcaaa cttccagtca acggggaacg ccgcaccgcc
     9061 gagaccgaca atgccgctct cgtgcagatc ttccacgaac gcggcgagat cctccgccgt
     9121 gcgcggggca agcgtgggat cgacttccat ttttccgtcg ttctcaatgc ggatgaccgg
     9181 cagatcgttg ccgaagagct ccggcatcgg atcgagcgag agaacggtgc cggaaacggt
     9241 cgcatgaacc tcacaggtga tgctgccctc gccgcgggcg agcttctgac cgacacggac
     9301 atagtcgccg acgctgacga ccggcacggc gggggtgccc atgtgcatgt tcatggggag
     9361 aagcacttct ttcggcgggg gaagggtttc ggccttcatt ccggcggtgt tcttgtgatg
     9421 gggaacgtga accgcctgaa ggcgcttacg gaatgccatc agaatccaac ctttccattc
     9481 atttcttctt tgctgtaatg cagtaatttt agctttgcga ttcgcgggac gtatcgtccg
     9541 gaacgagaat ggcctccata ccggaggtca tatgcagctc gccgtccggg agcacccaga
     9601 gcgcttccac atcgccgagc ttttcgcaga gcgcctcgcc gtcggcaagg ggcatgcaga
     9661 aaagcgcggt ggagagcgca tcggcaagac cgctgttttt cgtcacgacc gtgacggagg
     9721 tgaagagcgt ggagggatag agcgtatcct tatcgatgat gtggtgatac ctcacgccgt
     9781 ccacggtgaa ataccgttca taaatgccgg aggtgacgca ggaggtgtcg gcgagcagga
     9841 gctttgcgca gtaattcgag tcagggttct tcgggtcgcg cacgccggtc ttccagccgg
     9901 tgctgtcggg ccgcgtgccg atgatgcgga tgttgccgcc gacgttaagc acatagctct
     9961 cgcagccgag agtttcggcc ttctgcgccg ccatttcggt ggcgtagccc ttgccgagcg
    10021 cgccgacgtc gatggaggca agcgggtccg agatacggac ggtgttgttt tcctcgtcca
    10081 gctccagaag agagatgtcc gtgtgcttcg ctgcctcggc gagatcttct tccgcgggaa
    10141 cataggcgtt cttcggatct tccgacgcgg cttcacgcgc gttgtgccag agcgtgagga
    10201 cacttcccat catcacgttc atctctccgc cggtcagctc atagagctcc ttggcataga
    10261 gcagaaactc catcagccgc gcatccaccg ttacagcttc tccgccggcc attttgttta
    10321 ccgtacagag gttgtttatg ccggaatact cgtggtagat atcaaaaagc tggtgatatt
    10381 ccccgagtat atcagcaagt tcggcagaac agtccgaaaa ccgttctgcc gaatcgcctg
    10441 catagctgta aatataggtc accgtgtcaa agtaggtgaa atagcttttc ccctgcgcct
    10501 tgacaggttc ggcggaagct gaacagccgg agagaagaac aagagcgagc agagcgccgc
    10561 agagaagcgc ggcgaccgcg cgggtattct ttttccggtt catttgcttc gcggaaatta
    10621 ccgggcgttg ttcgctgccg tggcgatagt cgcgcgcatg ccggtgatat ccatcgtgca
    10681 ggaggcaaac agagtctcat cgacggtcac gtcatggccg ttgtgctcgg tggtctccat
    10741 ggcgctgacc tcatcggcgg tcatgccgac ggtgtagtca gcaaaagcct tggcctgggc
    10801 gtcccactcg gcaatggcgt tgccgtactt gaccatgttg tagtcgtcac cgagctcacg
    10861 cttggtagcg gtgtagtcct tgctgacgat ctcgccctcg gcgctcacgg tgatcttggg
    10921 ctggatggcg tcggtcagag cggcgagaat cttgccgtcg gagccgacag cagcggcgcc
    10981 gaactcggtg tacatcttga ccatggcgtt gccgtcttcg gaatcagcag cggacacgga
    11041 ctcggtggag ttggagatgg cggacacgcc cagcgtgaag gagccgggga cggcggtgaa
    11101 ggtggagccc ttctcgtcgt tgcaggcctt cacaatggcc tcctggaagg cctcgaggct
    11161 gatggagcag gaagcgaaca gctcttcgtt aacggcgacg ttgtagccct cttcgttgac
    11221 gcgggtctcg atgttcttga cctcgtcagc agtcttgccg acgacccact tctcaaggtt
    11281 ctcgacctgc tcaaaccact cgagcgtggc gtcggagtac ttgaccatgt tgtagtcata
    11341 cttcagatcg cgcttggtgg tgtaggtcgc ggcggtatcg acctggccgc cggtgacgtc
    11401 catcttgttc tgcgcgcagt cgatgcggca gagcacgatc ttgccttcgc tgtcggtgac
    11461 gaccgtggtg acggtggcgt cgacctgagc tttattggtg tcggagctgt caaggctgac
    11521 gctcacgccc atgccaagct tgtactcggc ttcggcggtc tcggcggcag gagcttcggt
    11581 agcttccgcg gtgggatcgg tggcgggggc agcgggctgg ctgccgcagg cagtcagcgt
    11641 gagcagcatg cacgccagaa ggagaacgga gattgctttt ttcattgtgt tcgacctctt
    11701 attcttaaat tttttgctta cgattccaaa cggaaaagcg aattatgttg attaatggta
    11761 cactgcgtta cgctatcccg cttttgatga caatataaca cagagcgcgt ttatagtcaa
    11821 taaaaaatca tacaagatag aggaaacaat ttaacctttt cggtaataaa ctgcacatat
    11881 atcgaacgct tgcgctaaat tgcaacaatt ctgtgaattt tccgcttgcg ccagtcgttt
    11941 aaaatatggt ataatctctt tagaatattc aataatggag aacattccgg ccggtttttg
    12001 ggtgcggaat gtttggcgat tgcattttgg aggtgcgaaa tgttttccat cggagagaag
    12061 gtaatgtacg gcggaacggg cgtttgcgtg gtggaggaga tcacatcggt gaaattctca
    12121 gcctctcagc cgggaaaacc gtattatctt ctgcggccga tgtaccagac cggcaccatc
    12181 cagaccccgg cggacagcgg caaggtcccc atccgcccgg tgatgaaccg cagcgaggcc
    12241 gaagccgtca tcgatgcaat gccgaccgtt ccggcggaga tctgcacgga gaagaatctc
    12301 agcgcgctgc gcaattatta ccagaagcag atcacctcgt tcgacagcat ggatcttatc
    12361 cgtctggcaa aatccatccg cgcgaagaag atagaagccg aaaagcgtca gaagcgcatc
    12421 ggcagcacgg acgaaaaata tctccgccgc gcggaggagc ttctcttcgg cgagcttgcc
    12481 gtcgcgctgg agatctcgat ggacgacgtt ccggcgttca tagaacagcg gctcggctcg
    12541 ctggctgccg cgctggagta actacatatt ataaaaagta atcatcgtct ccgaagagcc
    12601 ttttct
//
LOCUS       DAWAAE010000052        12380 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2291_length_12380_cov_2.734037, whole genome shotgun sequence.
ACCESSION   DAWAAE010000052 DAWAAE010000000
VERSION     DAWAAE010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12380
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_12380_cov_2.734037"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2459 a         3690 c         3492 g         2739 t
ORIGIN      
        1 agcatacgat ggacaaaggc gagctgaccg agatttttga taaatactgc aaaaagctgc
       61 ggatcgttcc ggcgtgggac gtgaagctgg aatttgtgga ggacccgtcc tggcgcaaga
      121 ccggcgattt caaggtggac tgcgaggata aaaaggccgt tttcctgctg aatgcggtca
      181 atccaaagca ggagaatctg gaagagatca tcgtgcacga gctgatgcat ctcaaaatgt
      241 acccgctcga tcaggtgacg gaatctctta tcgcaagcac ttttgaagag ggtactccgg
      301 cgagcaattt tgcttaccgc cagtttttca gcacgctgga gcagacggtg gaggagctgt
      361 cgaagtgctt ccttctggaa tttggcgacg atcgagagtt ctcctttggc cggtgcaagg
      421 acatgaagtc cttcaacgag ctgtacgacg gacttaaagc catcgattga caaacgccgt
      481 gcgctctttc cgaacgcggg acaatttttg cagcggttac aggataatat cgcaaatata
      541 agttaaaatt ttaattaagg agtatttttt gaaacttatc accttatcac cggagtgtgt
      601 ggcctgaccg ggaggtctgg caaatctaca cacgccattc ctcccgaaga ggctgaaaca
      661 catcttccgg aggagttatt atgaaccgcg aaaacaaacg cagatccttt gaaaaaggtt
      721 actataagac ccacgcctgc accaacgctt tcacctgcaa ggtatgcggc aggctctgca
      781 caccggagaa cgccggcagc gatcaccgca ccattgtcca aactgtctga gcagcctgca
      841 tgtggacatc gagcccggcg accgggaaac cgactgcggc agcatcatgg agccgatcgc
      901 cgtatgggtg cgcaagggcg gcgagtgggc aatcatccat cgctgccgcc gctgcggcgc
      961 gctcagttcc aaccgcgtgg cggcgaacga caaccctatg aagctgatga gcatcgccat
     1021 gcgcccgctt tgcgagccgc cattcccgct tgagcgtatc gaggagatga ccgcgctcat
     1081 gggcggcgac gggagtttaa agtaaccgaa aaagacacag cagcgcgggg atatgccgca
     1141 cggtatcgcc ctgcgctctt ttcaaatcaa aaaagcaaaa cagcaagaac tgtcgagacg
     1201 ggagatcgtc cgcttcctcc cgctgacaca gggtattgcc atgatgaaga acgcctttct
     1261 cggcatcaat gcggaaaacg tcctgctgcc ggtgcgcgtg atacccggtc tcacagcgct
     1321 ttgcaccgtt ctttccgtcc gctttttccg ccgggaataa cgttttgatc ccgaccgcat
     1381 aacgccgttt tctttgacag atcggaaaga aatctttata atttacaata gcgctgcatc
     1441 aatgaatatt caattcatac ggaggataac gctatgggaa tgtcgtttac aacactgagt
     1501 ctgtacggtg cggagcgctc cgctctcgaa gcggcgcttg cgccgggcga tctgctccgc
     1561 gaccggaatc tgccgtggct gacccttgtg ccggcgcagg aggaagaggg cgatttcccg
     1621 ctgcggctgg aaaaggccgc ccgccgtctg acgaagggct ccgacgcggt tgcactgctc
     1681 tttttttatt acgacgatga cgcttttgtc tgccgactgt atcgaaacgg tcggcgggcc
     1741 gcctcctgtc acagcgacga atcctgggca aagctgggaa aacagctcga cgagctgttc
     1801 ggcgacacat ccgcctccaa agcgttccgc tatgcttcgc gctgcaactc tctggaagag
     1861 aagctcgcgc tgctggagga gaccgtcggc gcggcactgt acgatgtgcc ggaagaggac
     1921 gcgccgcgcg tcgttccgcg ctgcgatgcc acgctgcgcg cggtaaaggc gcgggagtcg
     1981 atgctgcgga agcggcccaa gcagttcgtt ctcacggagc tcgcggaaga aagctggccg
     2041 gaggaaatga agatcatgca gacagtcttc cggctgctgc ggccgcaatg gaggcactgt
     2101 gagctcagca cgctgctgta tcaccccgac ccgcggccat acatgatgcc gggcgacacg
     2161 gggctctttg cctaccccta tatagatttt aagccgccct ataacgtaga tcagtcatgc
     2221 gagccgtttc cggaatttct ttttttgtac aacgcagaaa ccggagcgca gcagacactg
     2281 gggccgtttg ccaatcatgt ccggcgcatt atccggcgaa caaaaagcgg cgatcccgta
     2341 atgctttccg acggtctgcc gaaagtgtat cgggtgagaa gccgtgagct tgggcttccc
     2401 gttctgcccg cagtcacttg tttgggggaa gacggcacgg tgcggtggag cttcagcacg
     2461 gaggtcagta tcggttcctt tcatgccgcc cctgacgggg tcatcacgct tttcggaagg
     2521 gccgccaacg acagcgcggc gataatacag atcgacggcg agaccggaga gctgcttcgc
     2581 acaaggcatt ttcctcccga ggagggtatg cgcgaaatga tctatgccga gcccatcggc
     2641 gctctggtct gccgcagcga cgcctccgga gagcttgtcg ttctggacga agcgctcgaa
     2701 gaagtgcggc gcatgccgga cagcggcttt tcctgtgttt ccgaatttca cgggaattgc
     2761 ctttgggatt tctactatag tccctcgtcg atacgttttc tcgatctttc caacggttcc
     2821 gtgcacagca cgccgctgga gataccggat tttccgacca acgttctgcc ggacgggcgc
     2881 atactcggcg tgaacggaaa gcaggatcgg ctcaccgtgt tcgaccaaaa cggcaccatg
     2941 gtctcccgct gctccgttcc cggaaggctc tgcttttctt tcatctccgg cggagaggtc
     3001 tttctggcgg aaatgcgcgg gctggataaa tacggatggc tctgcgacga gctgttcgac
     3061 gaggcctcgg ctcatgagtg gcggcttgac cccgcgccga aaaaataaac atattccccg
     3121 cgcacgtcgg gcgaaccgcc gtacgcgggg ataaaccgtt ttcgggcatt gcttgacagt
     3181 ctgccggtcg gcaagtatac tccggggcga tttttttatg caaggacgga cggaaatgaa
     3241 aaagaaatcc gttctctttt gtctgctggc agtgctcacc gccgggcttt ccgtattcct
     3301 ccttgtgcgc ggcaaccggg gctccggcgc ctttcgcggc tcgttcgtga agaattccga
     3361 ttcctatttg ctaaatgcgg agcgcatgac cgccacagac acccatacac ttgcgctgca
     3421 aaagggtgat gtgctgcaga tccattttgc cgtggaaaaa ggctctctca agctgacgat
     3481 caccgcgccg gacggcacgg agctgtacgc aggcaacggc acggaggcgg aagatttcac
     3541 ggtgaacatc ccggcggacg gcgtctacat catctttgtc gatggccggc aggcagcggg
     3601 aacgctgcat atccagagaa ctgccgagta aatcttttca gccgagctct gttatggtaa
     3661 tcgcgcttcc atgacggcga aagactgttt ttcgcggaaa aacgtatttg caaacggcgg
     3721 gtcgtgcttt ataatggcag acggaaagtg aggtcatctc catgctgctc ggttctcttg
     3781 tcaatggcgc cgccattctc gccggttctg ccgtaggact gtttctccgc tttattctcc
     3841 gccgttcctc ccgccgaaac gagagcgaaa acgggatcgg agatcgtttg cagaagatcg
     3901 tcatgcaggg gctcgcgctg tgcacaatgt atatcggcat cagcggctcg ctcaaggggc
     3961 agaatacgct ccttgcgatc ctatcgctcg tgatcggcgc cgtgatcgga gaaattctcg
     4021 atctggacgg ggcaatgaat cgcgccggga actgggtgca gaaaaaagtg acgcaccccg
     4081 gcgaggaggg cgcagcctcg accgtggcag agggtttcgt taccgcatcg cttctcttcg
     4141 gcgtcggcgc gatgtcgatc gtcggctcgc tcaacagcgg gctgctgggc gaccacacca
     4201 tgctctttgc caaatcgctt ctcgacggcg tttcctccgt cgtgttcagc gtatccttcg
     4261 gcatcggcgt gcctctctcg gccattgccg tgtttgtgta tgaggcgctc atcacactcg
     4321 ctgccagcgt cctcgctccc ctgctcagcg acgccgtgat cgccgaaatg acctgcgtcg
     4381 gctcactgat gatcatcggt ctcgcgctca acatgctggg gcttacgaaa atcaaggtaa
     4441 cgaacttcat ccccgccgtt tttcttccga tcctgctttg ccggtttatg tgacggacgc
     4501 tcttgtatat gaagtgcccc caaaaaatta gacataaaat aggaagaaaa attatccaag
     4561 ccaccgaaag ggcttgcgat tccgcaaacc attgatacca ctatgatttt ttacttgcct
     4621 gtaacttgca acttggtatc aacttgcaac caacttgcga ctatcttaaa actaaatggc
     4681 atagaaaaag caccgtgcgg atgcatggtg ctttgtgatt tatactgctg tggactttaa
     4741 ctgctcctta tatctttcat agcttacctt ggcttctatc ggagcgtctt ccttgactgt
     4801 cggaatatgg ttcttcagct cccaccaatc gcgattgctc atccaataca tatccaattt
     4861 tttcattttt caccatttcc ttgcggatgc tttgtgcttc aatttttata taattctctt
     4921 ttctgctgca aatagtgttc aaagctcttt tttgcttctt ctggcgcatc ctctttcagc
     4981 ttgggaacaa aaccgtcata aaaataccag tctttatttg acttccaata tacatcaaat
     5041 ttcttcatag aatcagcccc ttaacagcct gatgaactct gtgacaagct ccgaatcctc
     5101 cgtctggctc tttatgataa cttcaggaat gatttctttg aacttcgttg cttttacagc
     5161 attcttaaaa gcatcacgtt caatttcacc gctgtactca accgcatatt ctgaaattgc
     5221 agtttccaga tacttaatac ttcaccgtct gaaagtgttt tttcgtatac attatagtat
     5281 gcgctcttgg cagtttcaag cctcttgttc ccaaaacaga tgcaaaatat ccttcgtcat
     5341 caaccggacg gcgatataat cgccgtcgaa gaaaacgcgt tccgtttgaa acttcagtcg
     5401 gatcggcttt cgctccggca ttggggacag gagctgcgcg cggagctttt ccagcgcctt
     5461 ccgccgtttg gcgagcacgc ttttgccgct cttctcccat tcgtccagcc caaagccgga
     5521 atcgatcatg gaaacagcgg cggttttgac cgcctcggtg agtatcccct ttttccacat
     5581 gaagtccgcc agcgaataga tataattgca ccactctatc gtgtcggact catcgccgac
     5641 ggaagtacgc acatactcgt cgagccggcg gacagcctcc tcggggtcgt accggtaaaa
     5701 ggcgcactgg tactcgaatt tcaggtcctc cgccgtgtcg tttctccaga cgcctgcgct
     5761 ccaggaaccc atgcgttcac tcccctttca gcgtgatcca gtaacgctcc agattttcat
     5821 tctcgttcgg ctcgtgctcg gtgctctcgt atacgccgcc cgcgcgcagg atcgtccggc
     5881 ggctcgcctc gttcgtatcg aggcaggtga tgagcacttt tcggagtccc atcgagcggc
     5941 agtaatccag tccctgcgcg agcatccatg aggcgtaccc tttccgccgc tcgtccgggc
     6001 gcaccgaata accgatgtgc ccgccgtagt gcaggagaaa gtcgttgagc gcgagacgca
     6061 cctgcagcat gccgacgatg cgcccgtcgc tcacacgctc gcagacgaac tgcgtgctct
     6121 gcaccttccc ctccgggacg gtggcggggt cggcgtactg cgcgttgatg gtcagccact
     6181 ccatcggatc gggcgtgcgg cgcatggaac cggtgccgtc catggagctt ccggcatcga
     6241 gaaacgcctg ccggtaggaa atatattccg cgacgtcctc cgccgtcggg agacggaatt
     6301 tcagcttttc gttcatggct cttccctctt ttcctctttt gtaaaaatcg tccgcgcggc
     6361 gaacgccttt accagcgctt cgtaccgcgg catgtccaca aggcagctca gcccatggcc
     6421 cgcgccggga aacgtgtgaa attcgatctt ctccggattt gccgcggcaa tggcgcggga
     6481 catatcgcac ggcacgaacc ggtcgtcctc gccgtgaatg agcaggatcg gtacccttgc
     6541 gctcttcacg gcggagagcg cgtccgcctg tgtgagatcg gcatggccga agagcttcgc
     6601 gctcaggcgc acaaagggat acagcagttc catgggaacg accgaatcgc gcccgacaga
     6661 gcagatgata tccttcgcgc tcgtatacgg gcagtcggcg atcacaccgc ggacgttttc
     6721 cggcagaccg agcgccgacg ccatgagcac cgtcgctgcg cccatggaaa tgccgccgag
     6781 caaaatgggg ataccgccga accgatcggc tgcccagcgc gtccagagaa ggacatcata
     6841 tttctccatc gcgccgaagg tgatcgtgtg cccggcgctg ccgcactgtc cgcgttcttc
     6901 aatgagcagc agcgcgcagc ccatagagcg gtagatctcc gccccgacgc tgaaatcgcg
     6961 cgagggcgtg ccgcggtagc cgtggcacgc gatcacgagc ggcgcgcccg gctttgccgg
     7021 ataataccgc cccgtgagcc gcagcccgtc ggccgaggtg atgtacacgc gctcgcacgg
     7081 cagcgcgttg agcgcgtcga tggcgcggga tatctcttcc cagtagacct gcatctgcgg
     7141 gtttttcgat atgtgatggt cgtcgttctg cgcgccgcgg ggcgaataaa atgcgtagcg
     7201 gtacagccca tacaatccgc cgagcgcggc aagcgccgaa gcggacgcgg cgcagagcgc
     7261 ctttttccgt tggatcacaa tcagcccttc tttcctgcgc attataccat aaatcgtccg
     7321 cagtacaatt aacattttcg tacgcgcttt attgacgact tcgcgttatt gcgttataat
     7381 tgttgcgcaa tattcgttgc acaataatta taatacaaag aaagaatgac cgaccttgcc
     7441 gccgaaagcc aaatttaccc gtcaggagat cgtggaggcc gccgtggata ttgcccgccg
     7501 cgccccgctc gaagccgtta ctgcgcagga gcttgcgagc gttctcggca cctccacgcg
     7561 cccggtgttc acccatttcc gcacgctgga ggaagtccgt gccgccgtag tggaggaagc
     7621 cgggaaaatc tacggacgat acgtggagcg cggcctttcg atgaacccgc cgttcaaggg
     7681 ctacggcatg gagaccatcc gctttgcgcg cgaggagccg aacctcttcc ggctgctctt
     7741 tctgcgccgg agagacaccg ccgagttccc gccgacggaa aaccacagcg ccgatatccg
     7801 ccgcgccatc cgggagacct tctcgctcac cgacgcgcag gccgacgagc tgcatcggca
     7861 tctctggatc tatgttcacg ggctctgcac gctgatcgtc acgggcgtga acgacatacc
     7921 ggacacggag gcggcccgtc tgctcggcga ggtgtgccgg gcgctgctca ttgcgcagcg
     7981 cgtgccgcgc gacgcgcgga ccgccatcgt tccgggcaag aaggttgacg ccggggcgct
     8041 cggcgagtat atcgccgcgc cggataagaa ataaaaagaa acgagaacag gagaactgtc
     8101 atgctgaaaa tcgaacacct taccaaggcc ttcggcgata agctcgccgt ggacgatctt
     8161 tctctgcaca tcgccccggg cgaaatttac ggtttcatcg gtcacaacgg cgcgggcaaa
     8221 accacgacgc tgcgctccgt tgcgggcatc cagcagttcg acaaaggcga gatctatatt
     8281 gccggacatt ccgtgcgcac cgagccgctc gagtgcaagc gcgctctcgc ctacatcccc
     8341 gataaccccg atctttacga ctttatgagc ggcatcaagt acctcaattt tatcgccgac
     8401 attttcggcg tcggcgccga cgagcgcgcc gagcgtatcc gccgccgggc cgcggagttt
     8461 gcgctgacgg acgatttggc gcagcccatc gccgcctatt cccacggcat gaagcagaag
     8521 ctcgcgatca tctccgcatg gctgcacgag ccgaagctca ttctcatgga cgaaccgttc
     8581 gtcggtcttg acccgaaggc cgcgcacatt ctcaagggca tgatgcgcga gcactgcgac
     8641 gcaggcggcg cgatcttctt ctccacgcat gtgctcgaag ttgcggaaaa gctctgcgac
     8701 aaggtcgcca tcatccgccg cggcaagctc gtgcgctccg gcacgatgga tgaggtgcgc
     8761 ggcgacgact cgctcgaaga ggtgttcctt gagctggagg aagacgtgaa atgacaaaaa
     8821 atctcctccg caagcagctc gccgagatct tccgcacgta tttgtacgat gcgaagaaga
     8881 acaaggcgcg ctccaaaggc acggtcatcg cttactttgt cctgtttgcg ctcctcatgg
     8941 tcgggctgct cggcggcatg ttcacttttt tggcattcac gctgcgaagc acgatcgtaa
     9001 gcggctacgg atggttttac tatctcatct tcgcgctcgc ggccgtatgc atcggcgcgt
     9061 tcggcagcgt gttcaacacg ttctccgggc tgtatctgag ccgcgataac gatctgctgc
     9121 tctccatgcc catcccggtg cattcgatca tggcgtcccg gctgctcacc gtgtttctca
     9181 tggggcttat gtattccggc gtcgtttcga tcccggcggc gatcgtatat ctcgctacgg
     9241 cggaattctc ggtgagcgct cttctcggcg cggtgctgtt tgtgaccctc attgccgtat
     9301 tcgtgctcgt gctctcgtgt ctgctcggct acggcgtggc ccgtctctcg ctcaagctca
     9361 aaaacaagag ctttatgacg gtcattttcg cgctgctctt cattgcgatc tactattttg
     9421 cctacttcaa ggcgggcagc tttatcggcg agatcgtggc gaatatagcg ctctacggcg
     9481 aggatctgca cgccgcagcg cctctcgttt tcggcattgg ccgggcgttt gagggtgatc
     9541 ttctccccct ccttctcgta acgctcgccg tagccgcgct ctttgcgctc acatggtaca
     9601 tcctctcgcg cagctttctc aagatcgcca ccgcgaccgg caagaccgac cggaaggtct
     9661 accgcgagac gcgcgcgaag agaaagagcg ccttttccgc catgctcgga aaagagttcg
     9721 gccgcttcac cggcagcgcg aattatatgc tgaactgcgg cctgggtacg cttctgctgc
     9781 ccatttcggg cgttttgctg cttctccgcg gcggcgttat cgccggaacg ctcgacagcg
     9841 tgttcgagat ggacggcgcc atgccggtgc tgctcacggc ggcggtgtgt ctcgtctgct
     9901 cgatgaacga tatggccgtg cccagcgtgt cgctcgaagg caagacgctg tggatctccc
     9961 ggtcgctgcc cgtcgacgca tggacggcgc tgcgcgccaa atgcggcgtg cagctcctcc
    10021 tcaccgcgcc gtgcgcggtc tttgccgccg tgtgctcggc gatcgctctt cgcgccggat
    10081 ttgccgccgg acttctcatg gcgctctgct gcgcggcgtt cgtttttttc tcgacgtatt
    10141 tcgcgctgta catcggtctg cacaacgtga atcttgtctg gacgaatgag atcaacgtca
    10201 tcaagcaggg ttcgcagatc atccttgccc tgctcgccgg gtgggccgcg ccggtgatcc
    10261 ttgtcttgcc gtatatgctc gtgctgcacg gcaaaatttc cggcgaggca tatatcgcgc
    10321 tgctcacgct tgtgctcgcg ctcgctgcgg cgattttccg ccgctggctg cgcacaaagg
    10381 gcgctgagcg gtttgaaaat ctctgataca tagacatacg aaaagcccgc cggaccatgc
    10441 attggcatgg tcggcgggct ttttcgcaaa atatttacag ctttaccggg cagcggtagc
    10501 gcggcttgcc aaggctcttt ttgccgtatt cgatcgcctc ggccgggcag cgggtaatgc
    10561 atgccatgca gtgcgtgcag ttatcctccc aaaccggatg gccgtcgtgc agcgtgatgg
    10621 cgcatgtcgg gcagagcttc gcgcacaggc cgcagccggt gcatttttca ttcacggtga
    10681 actgcttggc gtggacgagg agcggataga acgctttgtt cacgacgccg cttttgagct
    10741 tgtcggcagc gctcaccttc ggctgcggga acggctttct ctcgcggatg agctccgcca
    10801 gccggtcaag catgggatcc gccgcggcaa cgatcccgcg cgccttggcc tcgtccggca
    10861 cgtcaaacat cacaatatag ttttccggca tgatgacacg ggcgcagccg cggaacgtga
    10921 gatgcttctc cgcgcagagc tcggcggcgt atttccccgc ttcgccgatc tctccgccgc
    10981 aggtcataac aaaatagatc tccttgctgc cgcggagcag cgtgcggcgc agatggtcgc
    11041 gcacgatgtg cggcagctgc caggcatacg tcggcgtgca gacgatccac ggcgtttcgg
    11101 attcgagcgg cgacgtatcg ttgtgccgga tgcgctccat gagcggcatt gtcttctcgc
    11161 cgagcgcggc ggcgagcttc tttgcggcgt attcgctgtt tcccgtaccg gtaaaataaa
    11221 ggatcatggc gttactcccc tttctccggc gaaagccgga ggaatattaa gtacagtata
    11281 tcacgcgctt tgccgcgcgg caaggtcatt ccgaaagatc gatccccgcg gccttcgccg
    11341 cgagcgtata gagcaccggc tcaaaccgca cgccgtacca gtgctccggc tcggtgcgcc
    11401 ggatgcacgc gaggacgaac tccgccgcgg cctccgccgc cgggaacgct tcatacccgc
    11461 ggcacaaaag tcccgtgaac gccgaggcga agagatcgcc cgtgccgtgg caagtccgcg
    11521 caaggcgcgc atggcggtag taaccgcgaa accccttccc gctcactacg acgccggtcg
    11581 tctccggctc gtagctcacg ccggtgagca cgaccgtgcc gacgcccttt tcatgcagcg
    11641 cggcggtgag ctcttcaata tacgcctcgt catattcttt gcggtactcc atgccggtga
    11701 gcagcgccgc ctccgtgata ttcggaagca ggatgtccgc agagaacgcc agcgtcttca
    11761 ttttctccac gaaggcggca tcaaaccccg catagagctt tccgttatcg gccatggcgg
    11821 gatcaacgat gcgcgcgccg cccggcacga gcagcgtgtc catgatgtgc cggacatggg
    11881 tgatctgctc gccgctgccg agatagccgg tgtacaccgc gtcgaagcgg aacccctctt
    11941 tctgccaatg ctgcgcaatg gcgggcatgt ccgccgtcag gtcgcggaac gtatagcccg
    12001 cgaagcctcc gccggtgtgc gtggagagta ctgccgaggg cagcaccgcc gcttccacgc
    12061 cgcacgccga caaaaccggc agcgccaccg tgagagagca ctggcccaca caggagatgt
    12121 cctgcaccgt taagacccgt ttgttcatac cgtttccttt cgttttttga aaagccaaaa
    12181 ggctccccgc ttatgcgggg agcttgagcg tgtcaaaaaa gtatttttga cacgctctca
    12241 aacgagaccc actgcgttgg gctctcgttt gaaggggctg cggctcgccg cagcgcactc
    12301 gctgcgctcg cacgtttgcg agccgagaag tatttttgcc gacgtacagg tcgccggcaa
    12361 aaatgattta aaccccattt
//
LOCUS       DAWAAE010000053        12080 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2357_length_12080_cov_3.264449, whole genome shotgun sequence.
ACCESSION   DAWAAE010000053 DAWAAE010000000
VERSION     DAWAAE010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_12080_cov_3.264449"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5209..5218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2558 a         3625 c         3519 g         2368 t
ORIGIN      
        1 tgcggagcag ccggagcgcg gaggttttca taaaacacgg tctcggtgaa cgccacatag
       61 ggcttcacaa agatcgacac accggggatc gtcgcaagga tctgccagcc gaggaagctc
      121 agctgcagca cgaaaagctc ccatttatgc ccgcgcatca tgagacgact ctcccgcatg
      181 cagcgtaccg gcgaccagtc gggatgatcg acctgcagaa acgacgccat ggcataggca
      241 tacgacagga tgaggccggg gacaaggagc gccgcggatg cgaccataac gagcgcatcg
      301 atcagcagcg tgagcgcgat ggcgcggccg gtgatggcga aaccgtcgag cagattgccg
      361 aagccggatg cctctccgcg cacgacacgg agctgatagg cgatatagcc ataagagagg
      421 acaatatcga cgagctgaaa gaggatcaga atcgcagtcg ggacgagctc cgcgttagcg
      481 aagtcgatac ggatcatgtt ctggtaatcg agcttctgga ctgcgttgag atcgatgagg
      541 atcggctgac cgccgatctt ctgcgccaga tagttgacga tccagatgaa tgccaacagc
      601 gccagcgtca cgaggcaggg atacggacgc ggcggatttg taaggatcag gctgcgggcg
      661 cgcagcttca tttctcctcg cgtcatgcct tatcctttcc ctcggcaaca aggcgttcgc
      721 cgatcttgta cacatcgccc gcaccgacgg taaggataat gtcgccgggg ctggccgttg
      781 cgcgcagcgt cttttcaagc tctgcgttat ccgcaaagaa gatgccgttc ggaagctcgc
      841 gcgcgatgtc cgcggacgag atgccgatgg tgttcttttc acgcgctgca tagatctccg
      901 cgagatagca cacgtccggg cggcggagct gttcaacaaa atcgctgaag agcgcctgcg
      961 tgcgggagta tgtatgcggc tggaacacaa cgatcgtgcg cttgtagccg agcgtttcaa
     1021 tggcgtcaag cagcgccttg agctcgctcg gatggtgggc gtaatcgtcg taaacatccg
     1081 caccgttgta cttgcccttg aactcaaacc ggcggttcgc gccggagaac ccggcgagac
     1141 cgtagcggat ggccgcaggg ctgcacccga gacagagcgc cgccgcagaa gccgcgagcg
     1201 cattcttcac attgtggata ccgggaacac ggaggcttac atgcgtgaac acctcttcgc
     1261 agtaaatgac gtcaaactcc gtctctgcgc cgtgctgcac gatattggcg gcgtagacat
     1321 ccgccccctt gttcaggccg aacgtgaaga tatctcggcc aagcggcgcg agagcgtcca
     1381 tggtattttt atcctcggcg ttcgcaatga tgtatccgtt ttccggcacg agcgaggcga
     1441 acttccggaa cgacgctttc acatcctcga gatccttgaa gaagtcgaga tggtccgcct
     1501 cgatgttcag gatgaccgcc acggtgggcg aaaacgcgtg gaaggaattg taatattcgc
     1561 acgcctccat cacgatcgtg tcgcccgcgc cgacacggtg gccggcgtgc agcagcggca
     1621 gcgttccgcc gatcataacg gtcgggtcgc gctcggcggc catgaggatg tgcgtgcaca
     1681 tggaggtcgt cgtcgtcttg ccgtgcgtgc cggagatgca gagcgagttg cggtaatcct
     1741 tcatgatcgc gccccacgcc tgcgtgcgct caaacaccgg aagcccgcgg cggtgcgcct
     1801 caacgatctc cgggttttcg tcgtgcacgg cggcggtgcg gacgacaaat tcggcgtccg
     1861 ccgggatgtt ctccgcgcga tgcccgatga acacgggaat gccgagcgag cgcagatgcg
     1921 ccacgttcgc cccctcgttc atgtccgaac cggtgatgac aaggcccgct tccgcgagga
     1981 cctccgccag aggcgacatg gatacgccgc cgacgccgat cagatgaccg cggcgtcccg
     2041 cccagtaata ttccgaaaac gatggcatgg acatactctc ctaaccaagt tcaaattgcc
     2101 tgaaaaggga ttatactata tctccaacga aaaaacaagt tattcccaat ctttggcacg
     2161 ctccacggcc ttatgccagc ggcggcagag ttcctgcgcg ctttccgcgt ccattttcgg
     2221 gcggaaaatg tgcgccgatt cgcggatgcg gacgatatca ccgacattcg accacactcc
     2281 ggcgtgcagc ccggcgagga acgccgcgcc gagcgccgtt gtctccacca taacgggccg
     2341 gtcgatggga cagcggagga cgtccgcctg aaactgcatg agaaagcggt tgacggaagc
     2401 gccgccgtcc acgcgcagga tctgcatggg cgcgccggcg tccttttcca ttgcgtcgat
     2461 gagatcgcgc acctgatagg cgatgccttc aagtcccgcg cggacaacgt gcgcgcgcgt
     2521 cgtcccgcgg gtgataccga cgagcgtgcc gcgcgcgtac atatcccagt acggcgcgcc
     2581 gaggccggtg aacgccgaga caagatacac gccgccgttg tccggcacgg actcggcaag
     2641 cgcctgcgtt tcctccgccg aggaaatgag atggagctca tcgcgcatcc actgcacggt
     2701 gctgccggca ttgaatacgc tgccctcgag acagtaattc gtctcgccgc ggatgctcca
     2761 cgccacgcac gagagaagcc cgcttcggct caccgggacg gtcgggccgg tgttcatcat
     2821 ggcaaagcag ccggtgccgt aggtgttttt cgcctcgccc ggatggatgc agccctgccc
     2881 gagcagcgcc gcctgctggt cgcccgccgc accgcagatc ggcgtgccgg agagctcctc
     2941 gagccccggc atatgcgagt cgatcgtgcc gtactcctcg gagttggaca ccggcttcgg
     3001 gagcatgcac atcgggattc ggagcgcttc gcagagctcg gtgtcccatt gcagcgtatg
     3061 tatattgaag agcatcgtgc gggaagcgtt gctgtaatcg gtcacatgga cacgcccgcc
     3121 ggtcagattc cagatgagcc aggtgtccac cgtgccgaag agcagctcgc cgcgctcggc
     3181 gcgcgctctc gcgccgggga cgttatcgag gatccagcgc agcttggtgc cggagaaata
     3241 ggcgtcgatc agaaggccgg tcttttcgcg gatcttctct tcccagccat cggcgcgcag
     3301 ctcctcgcac agaggcgccg tccggcggca ctgccagacg attgcgttat acacggggcg
     3361 gcccgtttcc ttttcccaga cgatcgtcgt ctcgcgctgg ttggtgatgc cgacggcggc
     3421 gatgtccgcg gcggaaaggc cgctcgtgcg gaatgcctcc gagagcgatt caagctgcgt
     3481 atagaggatc gtcatcggat cgtgctccac ccagccgggc tgcggataga tctgcgcgaa
     3541 ggggtgctgc gagagcgaca cgaccttccc gttcacatca aaaataatgg cgcgggagct
     3601 cgtcgtcccc tggtcaaggg cgataacgtg ctggctcatg ggataacctc cttgattttc
     3661 gctgggaatt gtatataata tattttataa tataggcatt atagcacaag aaaggatctt
     3721 tggctatggc acgtatgtcg gaatttacat ttttatcttc ggacgggcag acggcgctct
     3781 ccgcgcgtga atatctcccg gagggagacc ccgtcggcat cgttcagatc gtgcacggca
     3841 tcgccgagca tatcgcccgg tacgacgtgt ttgccgcgtt tctcgccgaa cacggctaca
     3901 tcgtggtgct gcacgaccag ctcggccacg gaaagagcgc gtcggacgag gcgcatctcg
     3961 gctttttcag cgaggaaaac ggctgggaaa aggcggtgca ggatatccgc tcgctgcacg
     4021 acaagacggc ggcgaagtat cccggaaagc cgtatttcat tttcggtcac agcatgggct
     4081 cgttcctgac ccggacatac ctcatccgct tccgcacggg gctggacggc gcggtcatca
     4141 gcggcaccgg acaccagagc cccgctctcg tatcggcagg caaggtaatg agcgccatcg
     4201 atattcgccg gaacgggtca atgcacaaga gcgattttct caacaagatg gcgttcggca
     4261 cctataacga caagtttgaa aatccccgca cctcgagtga ctggctcagc cgcgacgaag
     4321 ccgccgtgga cctctataac gaagacccgc tctgcggctt tgttccgacg gcggggctgc
     4381 tgcacgacat gatgaacggc atcgcgttcg tcacccagcc gaagaacatc gcccggatga
     4441 aaaaggatct gccgatcctg ttcgtctccg gcgactgcga ccccgtcggc gagcagggac
     4501 gcggcgtgat ccgcgcctac aaatgcttcc tcaaggccgg catgaaggat gtcaccatga
     4561 agctctatca cggcggaagg cacgaaatgc tcaacgaaac gaaccgcgcc gaggtatacg
     4621 aggacgtttt gagctggctc aacagcaaga cgggaaaata atgccggatc gtcccggcac
     4681 atagggactt gccgcgccgg aatacaatga accatccgtt aagggaggtt tgctgtatgc
     4741 tggcaagtgc gtttctactg ctgctcaatg gcgcgtttct ctcgctgcgg ctcggtgccg
     4801 gttccggctc gtttccgcgg ccgctgcctg ccaaggagga gcggcgctgc gtggagcgct
     4861 ggatgcaggg cgatctcgaa gcgcggaaca cgctcatcga gcataatctg cgtcttgtag
     4921 cacacatcat caagaaatac tatacgagcg agagcgagca ggacgatctc atctccatcg
     4981 gaacggtcgg tctcatcaag ggcgtttcga cgtatcgccc ggacaaaggc gtgcgccttg
     5041 cgacctacgc ctcccggtgc atcgagaacg aagtgctcat gcacttccgc ggcagccgga
     5101 aaagcgcggg cgatctctcg ctctccgact ccatcgacac ggattccgac ggcaattccc
     5161 tgtcactgat cgacgttctg gccgacgagg aggatatgtc cgagcgcgnn nnnnnnnngt
     5221 cagcatctgt cgcagctgat cgacaacgtg ctcgacgagc gcgaggccga gatcctccgc
     5281 cttcgctacg ggctgaacgg agacgccgcg ctcacgcagc gcgagaccgc caggcgctgc
     5341 ggcatttccc gctcctatgt atcgcggctg gagaaaaagg cgctcgaaaa actcaaaaga
     5401 aaactggaag aggaataata tgaacacgcg aaagacgatc gggatcattc tcatggtcct
     5461 tctgctgata ctttgcgcgg tgctgatggt tctgcgtttc agcgcagcca aggcgtccga
     5521 cgccgcagca ctccgggagt ccatggcaac gcccgtaccg accgcaacgc cggatgttac
     5581 cccggagccg agcgctacgc cggaacctac agtgacgccg gagccgaccg ctacgcccga
     5641 gccgacaccg gacccgaact cccctgccgg ccgcgccgcg gcgctcggtc tgccagagcc
     5701 gccggacatt gatatcaaca gctgggagtt tatcctcgca aacgccgata actccatcgc
     5761 cgagtacgcc ccgccggagc ttgtgacgct cgaagggcag ctcgtggaca gccgtattgc
     5821 cgatgcgctc gccgcgatgg cagcggacac ccgcgcgcag ggtctctccg tctatctctc
     5881 ctccggctac cggagttatg cagatcaggc cgcgaacttc ctccgcgtct gccggaacaa
     5941 cggcgtatcc gacggcaagg acagtcaggg ctactatatc accatgcccg ccggtcacag
     6001 cgagcaccag accggccttt gctgcgacat cacggatatc tattatccga cgaaaaaccg
     6061 ctccattgag aataccgcca tgttccagta catgaacgcg cattgtcagg agttcggctt
     6121 catcctgcgc ttccccgacg gcatggagcc gatcacaggc gtaatgtatg agccgttcca
     6181 tttccgctat gtcggcgtag aggccgcgaa gtatattaca gagaacaata tctgctttga
     6241 aaccttcgtt tcgctctacc gtgacatcgg caccgcggaa aatccgcagg aatcgtaaaa
     6301 aaagacttgc atttgctcac accttgtggt agtataacaa agctgttaga aatccgggtg
     6361 tggcgaagtt tggtatcgcg cctgaatggg gttcaggagg ccgctggttc aagtccagtc
     6421 actcggacca tatcgagtgt tcttaaggga tctttcctta agaacactcg atttttttgc
     6481 aataagccaa agcgcgggcc ggatgcccgc gctttggctt tttttgaaaa atcctgccca
     6541 aatcttacaa aaggattgct atcttccgcc ggactgacta aaatagacgg gaacactgca
     6601 aagggagcat ttaccatgga tcaggataag ataaatattc tgcgcatctt tcagctggac
     6661 ggaacgcctg tctcctgcga agactacggc agcgggcatg tgaacgtcac atttctcgtt
     6721 gtgaccgaca ccggacgccg gtatattctg caaaaaatca atcaccacgc ctttcgcaat
     6781 gtacaggcgc tttcggaaaa cattgcggcg gtgacggatt ttctgcgctc caagtccagc
     6841 gatccgcgca gcgtcatgtc cctcatccgg acaaaggacg gtgcgcccca cacccggtat
     6901 ggtgacgaat actggcgcgt atacgattat gtggacggga cgatctgcct tcagcagccg
     6961 gagacggacg aggatttcta tcaggccgcc gtcggtttcg gtacgtttgc gcagctgctc
     7021 tccgacttcc cggcggaaaa gctgcacgag accatcccga attttcacaa cacgcccgac
     7081 cgctgccgcc ttttccgcga ggcgatcgag cgcgacgcgg tgggccgcgc cgcggccgtt
     7141 cagccggaga tcgattttgc tcttgcaagg gaaacggaga tgtccgcgct ccagaacctt
     7201 ctttctgccg gagcgatccc gctccgcgtt gcgcacaacg atacgaagct caacaacgtt
     7261 cttctggacg cgaagacacg aaaggcgctc tgcgttgtgg atctggacac tccaaggtgg
     7321 aaatgagtct ggagcgcttc cgcgttttca cgcgcggata tgtccgtgcc tgcccgaatc
     7381 tgaccgccag ggagctggag ctccttccgt tgggggcgaa aacaataacg ttggagtgcg
     7441 gcatgcggtt tctgaccgac catatcgacg gcgatctgta ctttgccgtc caccgcgaag
     7501 ggcagaatct tgaccgtgcg cgggcgcagt tcaagctcgc ggcggatatg gaaaagaaat
     7561 gggatgatat gcagcgtatc gtcgccgagg aaacgaaggc gtaaattcaa acataccacc
     7621 ggagatcaac gttcacagga ggatgacatc atgcttttta ccaatgccaa tctatttctt
     7681 gacgggtgct ttcagcgcgg cgcgttccgc gtggaaaacg ggcgttttgc ggaaattctc
     7741 accgatgccg ccggagacgg gatcgacctg catgggcagt atgtgatccc cggtctggtg
     7801 gatatccaca gccacggtaa ttccggcgcg gacttttccg acggcgacta cgacggtctt
     7861 gtcaaaatgg caaagtatct cgccgcgaac ggcgtcacga gctttgcccc cgcgtccatg
     7921 acgctgccct acgacgtgct cgaagccgcg tacaaaactg ccgctgcgct caaaaaagcc
     7981 gcgcccgagg gctgcgcacg gctcatgggc atcaacatgg aagggccgtt cttctccgag
     8041 aagaaaaagg gcgcgcagaa cgcaagctat ctccgtctgc cggactacgc cgcgtttgaa
     8101 cgcctgtata atgcctccga aggtctgctc cgcatcgcgg acattgccgc ggagctgccc
     8161 ggcgcggtcg agttcacgga aaaagcctcg aagctctgca cggtgtccat cgcgcacacg
     8221 gactgcacct atgaggaagc cagcgccgtt ttcgatgccg gtgcttccca tctgacgcat
     8281 ctttataacg ccatgcccgg catccaccac cgcaagcccg gccccatcgg ggcggggtcc
     8341 gagcgcgtaa acgtcgttgc ggagctcatc agcgacggat accacatcca ccccagcgcc
     8401 gtgcggatgg cgttcaggct cttccccggc cgcatctgcc tcatttccga cgcgctgcgc
     8461 tgctgcggca tgccggacgg gcagtatacg ctcggcgggc aggacgtttt cctcaagggc
     8521 ggcgttgcaa agctcgccga cgggacgatc gccgggtccg ccgccaacct ttatgaatgc
     8581 atgcagcgcg ccgtatcctt cggtatcccg gcggaacagg cgatcctgag cgcaacgatc
     8641 atccccgcgc gcgagatcgg tcaggatgcg gagatcggct ccatcgccgt cggaaagctc
     8701 gccgactttg ttgtgtgcga cgccgcgctg aaccggagcg ccgtatatct cggcggacag
     8761 gcgctatgaa aagctatacg atagcaaaag catccggcgc gcccggctgg gacgcgatcg
     8821 atccgctgcg cgcggactgc gtgctgtggg agccggactg cggcgtccgc atggagcaga
     8881 agctctgcta cgacgattcc gcgctctatg ttttccagcg cgcatgggaa tcggacatcc
     8941 gagcggagtg cagcgccccg ctcagccccg tccatgagga cagctgcatg gaatttttct
     9001 tctcgctcac ggacgatggg cgttatgtaa acttcgagat caaccccaac gcctgcatgg
     9061 agctcggttt cggcccgaac cggcgcgagc gcgtccggct ctgccacaag tccgaacggg
     9121 aaacgttccg tcccgtctgt acccggacgc cggacggctg gacggcggaa taccgcatcc
     9181 cgctctcatt cctgcgcatc ctctatccgg agttttccct gcgctccggc gttttcttcc
     9241 gtgccaactg ctacaaatgc ggcgacaaaa ccgtgcatcc gcactttctc gcgtggaacg
     9301 ccgtggaaac ggcagcgccc gatttccacc ggccggagtt tttcggaaaa atgatcctgg
     9361 cgtaacaaaa aatccttcgg agcgtctgcc ccgaaggatt tttttattgc tctgctttgc
     9421 aaaattcgag agaaatatag taggcatagt cgtttaccgg cgacacgctt ccggctgcga
     9481 actcctgaac tgctctgacc tgaaggtcat tttcgctgta tatcagggca aggagcggct
     9541 gctccgcctc aaacgttatg ggcgtttcgt cctcaataga caccccggcc ctggcgaaat
     9601 actttcgatt ttcctcgccc tccagtatgg gaaagctcgt cgaaagtttt gacccaccgt
     9661 cggcgaggat gagcttcacc tggaaatttt caaaatccgg gacgatgagg atcgtcccct
     9721 ccttcggcga accgatgtcc cggtagctga gctcccagtt ctctttgctg acctgtttcc
     9781 cgaaatgata ctccgtcaga tagatacgca gggagagaaa atcgtccgag gctgtatagc
     9841 ggtacatata ggctccgtcc agaccggcga ggagcttgag cgtctgcatt tccgtgcttt
     9901 ctttttccac ggggcggatc acgttctctg gccggttcgt ccggatcagg tacgccccga
     9961 tgatggagag cgccgcagcg gaaacgatga gcagcgcagc gaccatgcat ttgagccgct
    10021 tttgccgccg ctttccgtcc gccgccgtct gaaggatatt ctcctctgcc cggcgcggca
    10081 gatcgttttc cgcgatatca tcgcccgaaa ggaattcgtt gatgccgatg cccagcgccg
    10141 cgcacagctc cgtatagagc gacacgtccg gcagacatac cccgcgctcc cacttggaga
    10201 ccgatttatc gctcacgccg agcttttcgg cgagctgctt ctgcgtgagg ccgagcgcct
    10261 ttcttttgcc cgcgatgtat tgtccgatcc tgatgagatc cataaaaccg ttctcctttg
    10321 tgttgatcgg tttctttata gcagaaccgc ggtgaaaaga acaccccctg aaagtagaat
    10381 cgtatctttt cacggcgaaa tgcgccgcgc ggcgggcgtt tttttcttta caaaccgaca
    10441 aaatggatgc tatattgagt ttatacgatc tccggcaaaa acgtatcgga agaaggaaga
    10501 aagaatgaaa aagacgatca aaaaactggc tctgatactt ctcaccgccg ctcttatgct
    10561 gactgtgacc ggctgcggcg ccaatgacta ccagacagcc gtgcagctca tgggcagcgg
    10621 cgacgcggcc gcggcctccg cggcgttcaa agcgctcggt gattacaagg acagcgcggc
    10681 gctcgcctcg gcgtgcgact actcgatcgc gacggacgcc taccttgccg aagactacga
    10741 gcaggcccgc gcgcttttcg ccgcgctcgg cgactataaa gagagcgctt cgctcgttac
    10801 cgcatgcgac tatgcgatcg cgcagaacac ctatgacgcc ggagagtacg cgcacgccgc
    10861 ggagctcttc actgccctcg gcgattataa aaacagcgcc gctctcgccg cgcaggccgg
    10921 cgacagagca tttgccgaaa agctgctcgg aagctgggta tcgaacgaaa tggatgtatc
    10981 gtcgatcttc atcgactccc tatacgacgc cattgatgac gacgaatcct ccaaagcgct
    11041 tctggactgc atggagctcg gcgctcttcc cctgaagtac accatcgagt tcaccggcga
    11101 gggtacattc cttctggcgg ctgacagcga atctgccgcc gcgatgatcg acacgttcta
    11161 tacagctttt acggacggtc tcaccgccta tctggaaaaa gagatcgagc aggacgctgc
    11221 gaacaacggc tataccgtgg aaggacttat gcagacttac ggctgcacaa cgacgcgcga
    11281 actcattgat gccatgctgg aaatgcctct ggaagacttt atggccagcc tgcttcccaa
    11341 ggagactttg aaggagcttc tcgacagcgg caccgtcaac ggcgtgtatg ccgtgaaaaa
    11401 cggtgagatc gttctgacca tcggcaagac gcagagctcc gccgtctatg acgaggccgc
    11461 cggtacgctc tccgtcgttg acgaggacat tgccggtact gcgatcgttt tctcccgcgc
    11521 ataaggcata catcacaacg ccgcggccgc actttcccgc gcggaacgcc gcggaaacgg
    11581 cagcgcccga tttccaccgg ccggagtttt tcggaaaaat gatattggcg cagcaaaaaa
    11641 tccttcggag catctgttcc gaaggatttt tttggtctgc tttgcaaagt acatcagggc
    11701 aaggagcggc tgctccgccg caaacgttat cgacggcgca tcaaaaacag gattttgtac
    11761 aaatgcgcac ttgaaattct ccgcatcgtc tggtaaaatt accgtgtctg agcattaaaa
    11821 cggaatcgag gcgatcaatc agaacaaaga aggaggcttt tacacatgaa gggaaagaaa
    11881 atcgtatcaa cgctccttgc gctgctgctt ctcgccagtc tgcccgtgtc ggcgcacgcg
    11941 gcgacctggg acatcggcaa gggcgacatc accgtaaacg ccgagagcgg cgggcagacg
    12001 gtcacgcagg gaggcggagc cgctataccg gacagcgcgc ccgtcattac gggaacctct
    12061 aaggaaaaca acgtcaccat
//
LOCUS       DAWAAE010000054        11842 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2424_length_11842_cov_3.033002, whole genome shotgun sequence.
ACCESSION   DAWAAE010000054 DAWAAE010000000
VERSION     DAWAAE010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_11842_cov_3.033002"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2304..2313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7975..7984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8709..8718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2426 a         3500 c         3487 g         2399 t
ORIGIN      
        1 gagcggcact gtgatcgggc cgtaatgcag cagaacttcg ccgcagtgac acactccgcc
       61 gaaaatttgt ggtgcgcatt ttctctccgt tgtggtacaa taggcgcatc gaaacaaaac
      121 ggagaacacc atgacgagga tactttttat ttgccacggc aacatctgcc gcagcccgat
      181 ggcggaatat gtgatgaagg atcttgtatc aaaggccggg cgggcagacg agtttttcat
      241 cgcctccgcg gcgacgagcc gtgaggagct cggaaacggc gtttaccccc ggcacggcaa
      301 aagcttgccg agcacggcat cccatgtgcc ggtcacgccg cacggcagat cacgtcgaag
      361 gattatgtta actttgatct cctcatcggc atggatgagg agaacatccg gaacatgcgg
      421 cgcgtcttct ccggcgatcc ggagggcaaa gtctctctcc tgctcgacca cgccgggcgc
      481 ggcgggaacg tcgccgatcc gtggtacacg ggcgattttg aagcgacctg gcgtgacgtg
      541 aacgacggct gccgcgcact gctccgcgag ctgagttgaa tattatgtct acttaattat
      601 gtttatttaa ttatggcaac cgactgaaaa gcgttctgcg cagcaagaga caagaaaggt
      661 gcattttcgg ttctttccat tcccggacaa tgcgcgtcta tccctggctc aaggaaaccg
      721 gcacgatctt cagccgccaa atccgtggac gctctgcccc cgctccccgc cgggacgaag
      781 tgccgcgtgg aagttgtaat gaaccgaaag gagtaagata cacatgatca aattcggtac
      841 cggcgggtgg cgagccatca tcgccgacgg cttcacaaag gagaacatcc gcctgctcac
      901 cgcagggctg tgcgcgctca tggaaaaaga gggccacgcc ggggcggaga tctgcgtcgg
      961 atatgaccgc cgctttctct ccaaggaatc tgcgcactgg gcgtgcgagg tgctcgcggg
     1021 ctacggcttc cgcccgtacg ttatcaaccg ctcgagcccg acgccgctca tcatgttcac
     1081 cgtgaagcgc cggaacatgc cctacggcat ggccgtgacg gcgagccaca accccgccat
     1141 ttataacggc atcaaggtat tcaccgaagg cgggcgcgat gccgacgcgg aagtaacgcg
     1201 ccgcattgag gccgagatcg aaaagctctc ccccgccgat gtgaagagtg tggattacga
     1261 caccgcgctc gccgagggaa agatcgttga gggcaacccg atgaacgagt atctcgacag
     1321 catcctctcc gccgtgaatg tggaggcgat caagaaagcc tcgctgcgca tcggctttga
     1381 cccgatgtac ggcgtgagct ggtcaagtct gagcatgctg ctcacaacgt gccggtgcga
     1441 tctggaggtc atccacgacc gtcacgatac gctattcggc ggcaagctgc ccgcgccgaa
     1501 cgtcgatacg ctcaaaccgc tcgccgcgct cgtgctcgac cggcactgtg atctcggcat
     1561 tgcgaccgac ggcgacgccg accgtctcgg cgtcatcgat aacgaagggc agttcatcca
     1621 cccgaacaag ctgcttgtgc tgctctacta ctatcttgtg aagtaccgcg gctgggaagg
     1681 gccgtgcgta cgcaacaact ccacctcctg cctgctcgac cgcgtcgccg cgggactggg
     1741 gcagaagtgc tacgaggtgc ctgtcggctt caagtgggtc tccgcgaaga tgagcgagac
     1801 cggcgcgatc atcggcggcg agtcgtccgg cggcatggcc gtgcgcggcc acatctccgg
     1861 taaggacggc atttacgccg cggcgctgct cgtggaaatg ctcgccgtta cgggaaaatc
     1921 cgtctccgag ctcttcgccg agatcaccga gcagtacggc aacccctgct gggccgaggc
     1981 cgacttccgc atgacgccgg agcgcaaggc cgagctccag gacattctct ttgtggaaaa
     2041 gaaagtgccg gaattcggta cggctgtcga tcacatcagc cgggaagatg gctgcaaggt
     2101 gtacttcaag gacggaagct gggtcatctg ccgtttctcc ggcacggagc cgctgctccg
     2161 catggctgcc gaagccgatg tgcaggaagc tgccgacgga tatatcaagg cgttccgcga
     2221 tatgctggaa ctctgaaaac gtacaaagcc cttgggaata cttgcgtatt cccaagggct
     2281 tcagcaattt ttttacatca gttnnnnnnn nnnccggggc ggcaggcatc ggcgtaggcg
     2341 ctctccgcga ggaaaggcag gtcctcttcc ttcagcgctt ccagcttttc ggggataccg
     2401 acatccttgg agagcttctt cacggcatcg accgcggcct tgcggtactc ggcctgggtc
     2461 atcgaatcga caccctccac gcccatggcg cgcgcgatct cgcggtactt ctcgccggtg
     2521 cagtcggcgt tgtactccat gacgatcggc agcatcatcg cgcaggcgac gccgtgcggc
     2581 gtgtcataca cagcgccgag ggtatgcgcc atggagtgcg cgatgccgag accgacgttc
     2641 gagaagccca taccggcaat atactggccg agagccatgc cctcgcgacc ttccttcgtg
     2701 cccttaacgg cgtcgcgcag gtgagcggag atgagcttga tggcttcaag atggaacatg
     2761 tcggtcatct cccacgcggc cttcgtggtg tagccctcga tggcgtgggt cagcgcgtcc
     2821 atgccggtgg aggcggtgag acccgcgggc atggagctca tcatctccgg atcaacgatc
     2881 gcaacgatgg gcatatcgtg aggatcgacg catacgaact tgcgcttctt ctccacgtcc
     2941 gtgataacgt agttgatggt gacctcggcg gcggtgccgg cggtcgtcgg gatggcgatg
     3001 atcggcacgc agggcttctt tgtgtccgcc acgccctcca gagagcgcac gtcggcgaat
     3061 tcggggttgt tgataatgat gccgatggcc ttggaggtat ccatgggcga gccgcctccg
     3121 atggcgatca ggtaatccgc accgacggcc ttgaacgcgg cgacgccgtc ggtcacattc
     3181 gcaatggtgg gattgggctt tatatcggag aatatctcat agctcagtcc ggcggcatcc
     3241 agaacatcgg tcacgcgggc gataacgccg tgacgcagaa gagtggggcc gcagcagata
     3301 agcgcctttt tcaggccgcg ggacttgact tcggcgggca gctcctggat ggcgcctgca
     3361 ccgtgatatg aggtctggtt gagaatgatt ctgtttgcca tagtaatgtt tctcctttcg
     3421 tgatatgaaa agcaacctat tactcactat gcccttgtta atcaacactt tcgagcacaa
     3481 ccacatttta gcgtcccata taccgcttgt caacacaaat actcgccaaa aatcacctca
     3541 ttttgcagaa atgcatcggc aaactggcga attttcgatc cattttattt catgcgcaca
     3601 ggtgttccgc accgcgtgct tcttccccat cgtcatttcg ccgcagagca gcttccctat
     3661 gagatcaagc cgaacggcaa aataatcgtc gagtggctgg ccatccggcg cgttatctat
     3721 ttgcgatctt ctcttccgtc attatgtaaa ctttatgagc ataaaaatct gcgcagagaa
     3781 tggtttttct gcacagattt ttatattatg ttaataaaat tatgtttact gtattatgtt
     3841 aattacagga taaatccccc gtccacggtg agcacctgtc cggtgacgaa gcttgcgtcg
     3901 tcgctcgcga ggtaggaaac tgcggcggcg atgtcctccg gccgtccgag acggcgcagc
     3961 ggcgtttcct cggcaagctg cggcagcacc tcgctcggca gggcatccag catgtcggtt
     4021 tttattacgc cgggcgcaac gcagttgacg cggatgtgcg tgggcgcaag ctcggcggcc
     4081 agtgagcgcg tgagcgcgat gagcgcggct tttgtggcgg agtacgtcac ctcgcagctc
     4141 gctccgcgca gcccccacat ggacgatacg ttcacgatgc agcccgcgtg ctcacggagc
     4201 atcggcggca ggacggcctg gatggtgtgg aacgcgccgt cgatgttcac gccgaaatag
     4261 cgccgccaca tctcctccgt cacatcctga aaaagcgcct gccccgcgac gcccgcgttg
     4321 ttcacaagaa ggctcacatg gccgaacgca tcctccacgc ggcctaccat gtcctccacc
     4381 tgcgcgcggt cggcaacgtc cgcccgaaac gcggcggcgc gcagtccctc ggcggtgagc
     4441 tccgccacga gcgagtcggc gcagtcctgc cggacgtgat agtttacggc aacggtatag
     4501 ccctcatggg caagacggcg cgccgtcgcg cgaccgatgc cgcggctcgc gccggtgata
     4561 agtgcgatct tttccatttt gtttcctccc tgtgatatgc gtttattgta ccgcagcaaa
     4621 gagcggaata caagtcactg ctcgctctcc gtctcgtatt ccccggtctc cttctcgccg
     4681 aattcccgcg ccgtttccgc aacaacggcc tcgtacccgg tgagcgcggc gaacgaggaa
     4741 aactgcgccg ccctgtcgcg ccgcggcatg ggcgggtgca catcggaaac atggtgcggc
     4801 agggtgatga tatcatcata gtcgcggcat tcctcgcgct tcatgccctg tgtcctccga
     4861 tctgtttatt ccgctcgcgc gtcgtcgcgc catcttcgag attcatgccc tttagtatgg
     4921 cgttgctgcc gtagcgccgc tggatggcaa gaatggcttc ctgccgccgg cgttcgcgct
     4981 cgcgcgcctt tggatccgca ccgggcgcgc tccccttctc ctccgcggca gaaaagagat
     5041 ccagctgctc accgctctcg accgcgcggg ggatcttatc ctccggcagg acgcggttcg
     5101 ccgcgaccgt cacacggcgg acaagaaggt ttttgtccgt aatgcggtcg tagagcgcca
     5161 tgaccgcgtc cgtgatctcg cgcgtggagg acgagtagcc tgcgagattc gccgttccgt
     5221 ggccatgctt cggcacacgg cggccatagc ggtctacatg cgtttcaccg gcgtactccg
     5281 cggcgcgggc gggatcggag agattttcga tgtcgtaccc gatcgtgagc gtgagctgat
     5341 cggtacacag gcgctttttc acaagatcga gcacgagaag gtcggtcatc tcgcgcacga
     5401 tgagctttgc cttgtgccag tcgtacggct cctgcagcac ctgcccggag ctgatgctgt
     5461 tggagtccgg ccggtaggcg cggatatccg cgatcgtgca cggctcccag ccccaggcgt
     5521 ggtcaatgag aagctccgcc tgcacgccga acgttttata gagcagctcc tcgttataga
     5581 aatccccgct cttcccgagc gagcagcggg caatatcgcc catcgtgtgc attcccagcg
     5641 cttccagccg ccgcaccgtg cccgcaccga cacgccagaa atccgtgaga ggccggtggc
     5701 cccagagatt gcggcggtag ctcatttcgt caagctccgc aatgcgcacg ccgtcacggt
     5761 ccggcgggat gtgcttggct tcaatgtcca ttgcgatctt gcagagatag agattcgtcc
     5821 cgatgcccgc cgtggcggtg atgccggtcg tatgcagcac ctcgcgcagg atcttccggc
     5881 agagatcgtg cgccgacatg cggtacgtgg gcagataatc ggtcacatcc ataaacacct
     5941 cgtcaatgga gtagacgtgg atatcctcgg gcgctacgta gtgcagataa atgttgtaga
     6001 ttccggcgct gtagcgcatg tagtgcgcca tgtgcggcgg cgctacgaca taatccagct
     6061 ccagcgcggg gtttgccgca agctccggcg cgctgcacga ttttccggtg aacgcatggc
     6121 ccggcgcggc ggcgcggcgc gcggcgttga tcttccgcac cgcctccacg acctcaaaaa
     6181 gccgggcgcg gcccggaatg ccgtaggcct tgagcgccgg agacaccgca aggcagatcg
     6241 tcttttccgt gcgggtcggg tccgccacaa caagattcgc cgtcatcggg tcaaggccgc
     6301 gctccatgca ctccaccgag gcatagaacg acttgagatc gatggcaaga tatgtccgct
     6361 ctcccatggc gcacctcctt cgcggttggc ttttctctat tatattagct tattgagcga
     6421 atttcaagag gaaggttttg cggcagcatt gtgcatttat gcaaaactcc ccgacgccag
     6481 aggcgtcggg gagaaaagat gtaatcggat tatcccttcg gcagaaaatc cagccggacg
     6541 atctgcattt cggcatagga aaagccgcct ttcggcagcc ttttccctat agagcatcgt
     6601 gctgtgatca tagcgtatgc ccgtaaaaaa gcgacagctt ttgccggaaa actcaaagaa
     6661 gtccgagcag aagctccgcg acgaacgcca cgccgcagca cgaggccgcc acggtgatca
     6721 gtccgccgcc gcgccaggcg ataagaatcc cggccacgag cgccgcagcg ccggcgacgg
     6781 gctgctccgt tgcctgcacg atggcgggga acgtcatcac cgcgagcgtc acatacggca
     6841 catagtacag gaacgagcgg aggaaccggc tgcggatggg ctttcggatg agcgtgagcg
     6901 gcagaacgcg gacaaggtat gtaacgaccg ccgccgtcag gatggagagg agcgtttttg
     6961 tcatgccgcc gcctcctctt ccgccgggcg cgggaacaaa acagccgcaa gcgccgaaat
     7021 gacgaccgtg agaatgatcg tccggttgcc ggaggaaaga tcccgcagcg ccggcagaaa
     7081 ggcgaacgcc gcgctcgcgg caaaggccga gagcacgcag ccgagcacca cgcggttttc
     7141 cttcgccggg ggaacgatga tggcgaggaa catgccgtaa agcgccacgc tcagcgcgct
     7201 cacgatgcgt gccgggagaa ggttgcccat gaagatgccg agcgccgttc cagccgccca
     7261 catggggatg gaggcggtca tcgcgccgta gaaataccgc ggctccacgc agcccggccg
     7321 ggcgatcgtg atgccgaaga gctcgtcggt cacatcaaag ccaatgaaaa accggtggta
     7381 aaacggcata tcggggctca tgcgctggct gagcgcgcag ctcataagga gataccgcgc
     7441 gttgataatg agcgttgcaa gagcgatctc cacacaggca gcgccgctgc cgatgagcgt
     7501 gaagagggcg tactcacccg ccgaggcgtt gcagagaagg ctggagagca gcccctgcgt
     7561 tacggtgagt ccggcattgc gcgcggcgat gccgagggaa aacgacacgg caaaatagcc
     7621 gagaccgatg ggaaagccgt cgcgcaggcc ttcaaaatag acgctgcggc ggctgttgga
     7681 atgcgtttgc ttcatagcgg cttctttctt aaggtaatag ggatacaaag agaacttgta
     7741 ccacatttgc cccgctctgg caaggacttt tccgaaagaa gcgaagaaaa tccatttctg
     7801 cttattcgtt ccggtctttg atctgccggt accggcagaa gaagcgggca aagaaataga
     7861 tatacagccc cacaattatg ccgagcagcg cggcgagaac gccttctccc ttctatatat
     7921 tgccgcgatg ctatttgtca accccttttc gcaccttgac agggtgtgta taatnnnnnn
     7981 nnnngtataa taattttata aagaaagagg agtggatacc attgaacagc ccccggcaga
     8041 agagcgagcg cacgctcggc attctgtgca ttctatgcgc ggcattctgt ttttcactga
     8101 tgtcgctttt tgtccggctc gccggaaaca ttcccacgat gcagaagatc ttcttccgga
     8161 atctggtggc ggcgatcgcc gcattcgtga tcctcgcgcg cgaggggcag gggttccgca
     8221 tccataaggg ctgtctcggc ggccatctgg cacggagcat caccggtatg ctcggcatgg
     8281 tcgccaattt ctgggccatc gatcacatcg gcatggccga cgccaatatg ctcaacaagc
     8341 tctcgccgtt tttcgccatc ctcttttcca tattcattct gcgcgagtac ccgaagaagg
     8401 tggacattct cagcgttctg gcggcgttta tcggcgcggt attcgtcgtc cgtccctcgg
     8461 cgggcattgc gagtctcccc tcgctcgccg gcgtgctggg cggtctcggc gcgggcgccg
     8521 cgtacacgtt cgtccggcag atgagccgca agggcgagcg cgggccggag atcgtgatgt
     8581 ttttctccgc gttcacctgc gtctgcgcca tcccgttctt cgctttcaac tttcagccga
     8641 tgagcgcgaa gcagtggctc tttctgctgc tggccggctg cgccgcggcc ggaggacagc
     8701 tttccattnn nnnnnnnnct ttccattacc gccgcgtacc gccacgcccc ggcgaaggag
     8761 atctccgtgt ttgattattc gcagatcgtc tatgcggcgc tctggggctt tctcatcttc
     8821 ggcgagatcc cggacgcgct gagctttgtc ggctatgcga tcatcatcgg cgcggcggtg
     8881 ttccgctgca ttttcaatct gcgcgcggac aaaaaggccg tgcagagcgc cgcagctgaa
     8941 taagaaacca tatgcaaaac cgcccgaacg aactgtttcg ttcgggcggt cgtttattct
     9001 gtttacagcc agctcaacac tgtattgcag aggtacttcc ccttgatata cttgccaagc
     9061 tcacgcgctt tggcgtaagc gctcgcggct gcggcatggg aaaaggcgcg cacctcttcc
     9121 ggcgaagcgc ggcgctcgta cagcttctcc cgctcttcaa gaaatccctg ctgcatctcg
     9181 tcaaaaatgg ggatcacaag gtcattggcg atctcatcgt accggcggta gcaaagctcg
     9241 ccgatttcct gaaacgccca ccacgcggag ccgagatcgt actcgccgca ctcgccccag
     9301 gtgtaagcct ccggcaggcc ttcgcccgcc gtgaataccg gaacatagac cgcgagcttg
     9361 gctgccgcgc aggcgaccca catgcatccg gctgccggcg atgtatccgg ccgcagatca
     9421 tacaccatgg aaacgaccgt tccgccctgt cctacgcttg tgcttgttgt catccaaagc
     9481 gggttatgat gcacgcccgc ttccggtgca agggataggt caaaccgcgt gccctccatc
     9541 gtgcttgtga gagcatgcat cacgtcgcgc gccgtgagtt tgcgctgcgg tttggcacaa
     9601 taggccttcg caaagggatt ggaatccacg ttacagagcg tatgcagaca atcccagcgg
     9661 cgcatacggt tcaccggcca atccgcgccg ccgtaggtgt cgtttgcaag cgggcccgtg
     9721 ccgtatatct tcgtgaagtt gaacggctct ccctccttcc aaagaccgtt ttcaagagca
     9781 taggaacgga ggtttttaga gcaaatgaag ttcttcgtat cggagaaatc gatctcctgc
     9841 gtgccgaagc agttgggacg gcactcaacc tcgtcatcgg caacgcgctt ggccacccat
     9901 tttccgccgg gaacgagttc caggaaaaat ccttcttccg gatcggccac ggccatgcag
     9961 accgcgcccg ccgcgccgtc gatggaggaa acggtgtagc cgtattcctc gatcagttcg
    10021 ccgatcattt caatggcctg acgcgccgtg cggccgcgtt ccaggagcag aagcgtgatg
    10081 tcgtcggctt ccagaatgtc tttttcggtc tggcagttgc ggaattcata tacgcccatc
    10141 ataccgacag acacctgatt ctcgttcatg ccgtccgccc aggagatggg gcgcgtgccg
    10201 gtctcatacg cgcacgccgt ataggtgtag gcatacgttt ccgccgcctg cgggatatga
    10261 tggcccttca cggcaaggaa atattcgtcc ggtgtatgga cgatgtgcgg cttaaaatgt
    10321 gtgtggccgg ggcagccggg ccagtcgtcg tttgctccgg cgagcactgc gccgctcact
    10381 gtcgtatttc tgccggcaat aagaccaaag cacataatca attctccttt tctgccgttt
    10441 ccggcttata ggtcgctttc gcggcgataa agtaaatgat gcccgtggca aggaagctga
    10501 agagcacctg tccgatggaa tcgcacacgc ccggaaggag ctttgccgcg aaatagatcg
    10561 ccgcaccggc aacccacgag ataacgcccg tccagttgat gccgttttcg taccagtagg
    10621 ggccgttttt gttgttgaac tgcgtgatgt cgtacttctg gccgcggacg agataatagt
    10681 ccaccacaag caccgcaatg cacggcggga acactgcgcc aagatagcca aggaaaacct
    10741 ggaacgtatc caggaacgag ccaagcacga gcggcgtgag cgcaagcagc atcgagagaa
    10801 tgccgagccc gatcatcgag cccttgggcg gcagcttttc gctgatattc gccgtggaga
    10861 agccgccgga atagatgttg atcatgttga ccgcgatggt ggagatgacg atgagcagat
    10921 ttgctatact gcccatgccg agactgctgc agatgtagga aagatcggcg gagtacgggt
    10981 caaagagacc gccattcgtc acggcaacgg caatggcgcc cgtcgcgccg ataagcacga
    11041 accagaggaa gccgagcgtc gcaccgatca tcggcgcaac cgtcgcagac tttttgctcg
    11101 tggtatagcg cgtatagtcc gccgcgcaca tgacccacgc aaagccgttg gcaaggatga
    11161 tgtcaaggcc gccgaacgaa agcgaccagc cgctgttggc cggattatcc gtgatcgacc
    11221 atttccagat atccgagaac gacacgagag agaatatccg gataccgatc cacacgctga
    11281 gaattgcaag acacaccact gcaacaatct gcgcttttcc gatgtatttg gcgccgtata
    11341 cggaaacgaa cgtcgtaata agcagaataa taaagaccga aataataagc ataatcggcg
    11401 agtgaggatc gccgccgaag atgctgcaaa gaagagtcgc aagcggcgtg gcggcaatgt
    11461 aggtgttgac accgcaccag ccgacgaact gcagcgcgtt gatgaacgag gggagcttgc
    11521 tgcccgcgat gccgaaggac acgcggcaca ggcccatatt tgtggaggct accttgtaac
    11581 cgatgatacc gacaagagcg agaataaggt aaacgagcgg gtttccgatg atcgccgaga
    11641 gaagcgcacc tcccagaccc agcgcaccga ggatcgtgcc catgtaccag gtcgtcgggt
    11701 ttgcgttggc gcagatccag gtggaggtca tctcctttgc gccgtaatag cgtgcctcca
    11761 tgggaacata ttcgtcaata gcatagtcgc gaatttccac ttgctgtgta ttggtttctt
    11821 ccgccctgat cggttctcct tt
//
LOCUS       DAWAAE010000055        11731 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2450_length_11731_cov_3.214714, whole genome shotgun sequence.
ACCESSION   DAWAAE010000055 DAWAAE010000000
VERSION     DAWAAE010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2450_length_11731_cov_3.214714"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2631..2640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10469..10478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2460 a         3366 c         3611 g         2274 t
ORIGIN      
        1 cggtagtccg gcagcgcgac gcgcacgccg agcgcgcggg caagctctcc tgccagagcg
       61 aaatggtgcg gcgctgcgtt gaacacgaac ccgccgccgt ggagaaagta cacgcacggc
      121 gtctcgccct cgcagcccgc cggagcataa agaagcatac ggaggcagct cccatcggac
      181 cgcggcacgc tcagacgcga tacgcgcacc ccgccgccgc tttgctgcgc ggcgtataag
      241 agcttcatcg gcccatggga caggcgcacg aaccacggcg agagcgtgga cttcatatgc
      301 gcatagcgcg caaggtcggg atgataggta tagtccggca tggtttttct ccctttaaaa
      361 aagcgcccgc cgcacggcag aaagatcgtg cggcgggcgg tttttatgtt tctttcggct
      421 tttccggctc cttgcgcagc agcatgagca gcacaaagag gatgcagcag acgttgatgg
      481 cgatatcggc gatgttgaac accgcaaaat gcatgaactc cagctcgaac atatcgacga
      541 cgtagccgta gagcgcgcgg tcgatgccgt tgccgatcgc gccggccatg gcgagcaccg
      601 caagccagcg ctcccacggc gcggtgagct tatggcgcac gagcgcccac acaacgatga
      661 cgcagaacgc cgcgagcagc gcaaggaaca gccaccgtcc gcccgagaac atgccgaagg
      721 cgacgccgct gttgcggatg tgtgtgagat ggacaacgcc gggaatcagc gcgcgctgct
      781 gcgcggccga ggcgttgagc ggaatgttcc gcacgaccca tgccttgagc gcctgatccg
      841 ccgcgatgat cgccagcgag gcgagaacat acccgatcac gttttttttc ttagctgtca
      901 taccggctcc ttctgataat aaaagcaaag agccgcccgg catcgggcag ctcttcggga
      961 aataacttac ttcttatcga cagcttcctt gagagccttg ccggccttga actggggaac
     1021 gcgggaagcg gggatctcga tctccttctt ggtcttgggg ttgcggccca tgcggacgcc
     1081 gcgctccttg acatcaaaag cgccgaagcc gacgagctgc accttctcgc cctggatgag
     1141 ggtctcggtg atggtctcca gagaagcgtt gacggcgagc tcgctgtcct tcttggaaag
     1201 gccggtcttt tcggcgacct gagcaataag ttctgttttg ttcatggtta tcctcctcat
     1261 ttgtttacaa cctgcggtca gagccgcggc aaccgccgcg cgtccggccg cagtgacctt
     1321 tttctcagcg caaaaaaagc gtcttttttg tgcgcccgaa aaccctatca tattttttgc
     1381 cataacgcaa gaggaaattc ggcaaacgac cggttttgcg tatctttttg gcgaatatta
     1441 cccgtttccc cggcaaaata ttttttcagc gatctttctt tttgagatgc gctccgccgc
     1501 ggctcgcgct ccggcgcgtg ctgccggagg aaggacggcg cggcgtctcg tcgagcgcgt
     1561 gcagatgcag gatgcgcccg agcttttccc cgtgcgggat gagcgccata tcctccatcg
     1621 tgacggcgcg ggtaaggccg atcatcacga cgtacacgac caccgccgcg gcaatggcag
     1681 cgatcatcgc gcccgccatg gcgagccgct cgctcatgcc gcgcagaggg atgcgcgcga
     1741 gcccgtacac cgcccacgcc gcagcgccca tcgcaaggct cgaaaggagc ggtgcgccga
     1801 gcatgcgccc gagacgcagc cggctgccga gcgcccggca gaggaaaata taatccacga
     1861 tgcagatgat cgcaaagcag cagatgctgc cgaccggcgc gccctggatg ttgatggctg
     1921 ccgtgccgac gagcacccag ttgaccgcga tcttcacaac gccgcccacg gcggtgctga
     1981 cgatggggag acgttccttc ccgctcgcct gcaggatggc gttgcacatc atggacatgc
     2041 agacaaagaa cgatgcgacc gcgagcagct tcaaaaggcc cggcccggcc gggttgctgc
     2101 cccagtagat cacgcggaac accgggtcgg cgaggacgaa gagccccacc gccatcggca
     2161 tcgtgacgag cgcggagatg cgcagcgacg attccgccag atggcccgcc gcggcgcggc
     2221 tgccgcgcgt gagatgcccg acgattgccg ggacgatgct cagcgtgagc ggtgtgatga
     2281 agtacgacgg caggttatag aacgtctgcg ccttgccgta tacgccgtac agctcgcggg
     2341 cgcttttctc cacgaacccg gcggcggact gcagccggtt gaggcagagc gcgttgtcga
     2401 gcaggttgat gagcgagagc accgaggagc ctatggcgat cggaatgccg atgcgcagaa
     2461 atgaccggac gatcgtatcg cggctgtcgg ggatatccgc cgtgccgtag ggccgcgggg
     2521 ggtaattacg gcgcttgtag agcatcatat agatgagcgc tccgaccgcg cccgccgtaa
     2581 cgccgaaaat cgcgcccgcc gaggcgagcg gcaggggctt gtgcgcgcgg nnnnnnnnnn
     2641 cgcatcgcgc accacgcagc cggtcggctt gtgcgcgcgg atgagaagga aggcgagcgt
     2701 cagaccgacg acgagcttgc cgacttcctc gatcacctgt ccgaacgccg tggggatcat
     2761 gttcccccgg ccctgacagt agccgcggta ggcggacgta aggcacacga tcacgaccgc
     2821 gggcgcaagc gcgagaatgc tcggcgcggc gtccgcattg cccatcatca cggcaagctg
     2881 ccggttgaag aggagcatca tcagcgtgaa gaacccgccg atggccgcga gcgtcccggc
     2941 ggcggtgcgg aaaatgcgct gcacctgggc ggggcggtcg agcgtatccg cctccgagat
     3001 catgcgcgag agcgccaccg ggaacccggc ggtcgccagc gtgaggaaaa agctgtagat
     3061 gttgtacgcc accataaagt gggcgaagcc ctcgtcgcca agcagcttca tgctgccgag
     3121 cgggagttta tagaagaacg ccgcgatctt gacgatgacc gtcgttccga ccatgatcgc
     3181 cgcgccgtgg agataatttt gctttttggc ttccgacaag gcagtccctc cctttgcggt
     3241 ggtgtgccgc acgattccgc atgattaagg atatgcggta agtttatatt ttacaccaac
     3301 acgcgggaaa aggcaagcat ggcaagcctt ttcccgcata aagctgtgag agaagcgcag
     3361 gtcagtcgag atagggctgc gtcatgtcca cggcgaagag aatgctcacg ccgtccgcgc
     3421 cggtgcgcag ggtggtcttg tccgcggcga aggcggcgtt gtgcagatag tggacggcga
     3481 tcttgctgag ctccgcttcc ggcggcacgg tgagccggac ggtgttcacg aacgcttcgg
     3541 aattttccgt gcagcgcacg agtgcgccgg gcgtctgccg cgcggcggca ttggcggcgc
     3601 cgaggacgcg ggcatagcgg ggctggttga tttcgttgct ttctttcatt gtgtttttct
     3661 cctttcgctt cgcacggttg tgtttccgtg tacactttat atggctgcga gcggggagaa
     3721 ttgcaaccgc gccgcaaaat tttttgcaaa acgagccgcc ctgccaagca ggacggctcg
     3781 ttccggatac ccggttttca gtttatgcgg cagccgcact gaccgcagaa gacggtgccg
     3841 ggctttaccg gcgcgccgca ctgcgggcag acaacggcgg cggggagctc cggcgtgctg
     3901 ccggtgttcc catcggcgcc gtagccgcag ttatagcaga aacgcgcgcc gggagaaagc
     3961 tcaacgccgc accgcgggca gactctgcgg tgcgtgatct cctcatagaa ggcggcctgc
     4021 accagaccga ggaacagcgg cccaaacaga gaggcggcaa tcgtgaacac cacgctgaga
     4081 atgccgaaga ggacccctat atacgggatc gtggccagca gcgcaagcag gatctccgcc
     4141 gcggcgataa cgacgacata gagagcgtcc gcgccgaaca tcctggcctt gtagccgcgc
     4201 gttttctcgc ggctgacctt gatggcctcg gtgatgggca catcgggctc gtgcatgaga
     4261 atgtaaggcg tgaggcggta ttcgtaagta cggatgatgc cgaacacgat gccggccacc
     4321 gggatgagcg accacaggaa gatccagaga ctggcccagc ccatgccgca gagaacgcgc
     4381 ttgacggtct tccagtcttt gaaggtatca aagagctgcg tgacggcgat ctcctcgccg
     4441 cggtagccgc gcagataaat gagcgtcatc gccgtctgca gaagcatcga cacggcaatg
     4501 gaggccgcgg gtatcacgcc cagcagcgga acggaaagag cgctcagcag catggcgagc
     4561 agcgaaatgc cccagagcag aaacggcttt ttccgcagaa cggcaaaggc acgcgaatag
     4621 attgttttaa tggacaccat ttgacaattc ctcccccgaa atgtttgtaa gctcagtgta
     4681 tcatccggcc cgggagaatg caatacggga aacaggaaga agaaccggca aaagggaaaa
     4741 tgaactgcac cccattcctt agcaacaagt attttaccat acttgtctaa ctaatggggt
     4801 gcagttcaga tgtccgcggc gtctttctta tactctgagg cgctacacgc ggaaaccgca
     4861 cattcggatc gatccaaatg cgcggtttcc gcttatataa cttgtgtcgg acagcagaaa
     4921 gatcagacgt tctggctttc cttggatgcg tggcgcttgt tgacgagctg cgtgatgaag
     4981 tacaccggca cgccgatagc gcagaggatc acggagagaa gcattttctt cggcgcgact
     5041 tgagcggcaa acacgatgca gaggatgatc gcaataacaa gcgtgtaggg gtaacccttc
     5101 atcttgaagt tgcgctgcag gtcaggtctc ttcttgcgca gaacggcaag agaagcggcg
     5161 acaaaaacat ccatgatacg cgcgataaac acgcacatgc cgagcagcac gccgaagctg
     5221 ccgagccaga gaagaatcgt ggcaaacacg gcgtagcaga tgattgcaac atacggcgta
     5281 ccgtatttcg aatgaacctt cgccacagga taggggtaca gaccgtcacg cgccagagag
     5341 aaagcgacgc gcggcgtgga cagaagcata ccgttgatcg aggcaaggat agaggcaatg
     5401 gcgcacaccg cgatgatcgc accgccgaca ttgccgaaga tgacggacgc ggcagaggca
     5461 accgccttgg tgctctcggc aaggccggta acgccgaggg ccttgatgca gacataggtg
     5521 agcaggatgt acaccgtgac gatgctcaca agaccgatga tgatggcgcg cggaagattt
     5581 ttgtgcgggt tcttgatctc ctcactcatg tagcaaagct ggttataacc gccaaaggca
     5641 aagaatgcag ggactgccgc agcgattgca ccgccccagc cgacggattc ggaaacgacg
     5701 ggctcgaagc tctggctgcc tttgagcagg aacagaccgc cgacgatcac aacggaaagg
     5761 ccaagcagct ttgccacggt gagaccactg gtgatgccag agccggattt gacgccgaag
     5821 atgttgaaga gagccgcaca cacgatgatc accgtaccga cgatcttgca ggtgaccggt
     5881 gaaagctgga agaaataccc gatgtagttc gtgatcgcca tggcgtagag cgcaatgacg
     5941 gagacctcgt tgaccatttt gccccagcca tagaaatagg caaattttgg gccaaaggct
     6001 tcgcgcaggt agatataacc gccgcccgcc tgcggcatgg ccgggccgag ctccatatag
     6061 accatcgcca tgaggaacac gcagagaccc gcgagaatcc acgcaaggaa gatcgtgccg
     6121 ctgccctgcg cggcgagcat gatttcgttc gggacggtga aaatgccgga gccgatcacg
     6181 cttgtgatca tgatcataac gcaagggaac aacgtaagcg aacgcttgag ttctttgttg
     6241 ttatccatta tctttctctc cttcgataca gtttatcgga tggtttcccc tgtggctgtc
     6301 tggatggcat agtccgagag atcctggcca aagcccggca gatcgggaac ttcaaaatag
     6361 ccgtccttcg gctggtagtt gtgcttgcct aggcaagtga tgccgggtag cgtattgccg
     6421 acatggtgct cgtggatgac aaagttcggg atcgccgctt caaactggag cgagaccgcc
     6481 acggagatcg ggctggagca gacatgggtc tggacgccga cgtcgtaaag atgcgcgtgg
     6541 tcgcagatct ttttgcactc ggtgatgccg ccgcagttgc cgatgtccgg ctgcgccacg
     6601 gaaatcgcgc ccgactcaat caacggacgg aagccccaac gggtaaacgt gcgctcgccc
     6661 gtggcgagag gaatgctcgt gtttgcggcg atcttctcaa agaccttgct gttgagcggc
     6721 gtcgcaggct cctcgaggaa catgggacga tatttctccg ccatgtgagc aaactggatg
     6781 gagctgaccg cgtcggtcat ggcatggttc tccagaagaa tatccccgtc cggccccatg
     6841 agctcgcgga gccgtgcgat gcgctcttcc gctgtgttca tgatttccga gctgagaaag
     6901 ccagtcgttt ccagatagtt cacgttgggg ccgtgcttac gctgctcgaa gaagttgacc
     6961 ttgactgccg tgtaaccttc gtccagcgcg gctttgcaag cgtcttcata cttttgcaga
     7021 ccgccgtcgc ggttgtaggc cgggatgccc cagccgaact ggagctggct ggcgtaggtg
     7081 cgcaacttgc tgcgctgctt gccgccgaga agctcatata cgggcgcatc atagtgcttg
     7141 ccccggatgt cccagagcgc ggtatcaatt gcgctgaccg ccgccatgat gatggcacca
     7201 ttgccctgcg cccagaaagt atctttgtag agcttttccc agatcacctc gtggtgcagc
     7261 gggttcatgc cgatgatacg ggtcgcgtaa tccttgagaa gctcatacgc tgccgtggcg
     7321 ccggagacta tagccacacc cgcttcgccg agaccgtata ttccctcatc cgtgttgatg
     7381 cggcagatga tggggcgaac cggtcccgct ccctccgttt tcagtcgcag cagatctacg
     7441 ctcgtaattc tcatagcgtt atatcatcct tcctttccgt cggggtttac cgacataata
     7501 cataatacgt attacatatt atgtctggat tatagcctgt ccctgcggaa aggtcaatac
     7561 acgcatttca caaaaaggca cggcggattt gtacagattc cataggcgca taacgagggg
     7621 aaaagagctt gccattttgc aattgtctgt tataataaag aatattatgt aaaaataatc
     7681 aatgcaggcg tgataaaatg ctgaatgaat caaaacagac ctataaagcc gaaagcgtat
     7741 ctgagcacgt atacggtttg ctgtatgaaa agatcctctc cggcgagtgg ccggtagaca
     7801 gcaaaattcc caacgagttt gacctttgcg atgcttttgg cgctagccgg atctcggtgc
     7861 gaagcgcgat ccaacgtctg atctccatgg gaatgctgga agtgaagcgc ggcgacggga
     7921 catatgtaaa atctttctct ctgcaaaact atctgcagca ggcggtcccg ttcatcgtac
     7981 agagcgcaga gcggcaggag gttgcgcagt tccgcgaggc actggaggcg caggcgtgtc
     8041 gcctggcgat cgagattcat aacgacgcgg agatcgacga gctcgagcgg ctgttccatc
     8101 gaagcaacga agcctttctc gcacacgacg ttgcggaata cgtcgtgcgg gatatggatt
     8161 accaccgcca tctctgcaaa atgtcgcaca accatattct cgtcacgatg tgggatgctt
     8221 ttgtaaagcc gatgagtctt tccatcaagg agaacgtctc gcccctgctg cagtttgacg
     8281 cctatgtgga tgaccatcat ctgcatatcg cacaggcgat ccgcgtaggg aacatcggcg
     8341 aagctctccg gttgctgcac atcgttctgt acggcctggc ataagaatcg attaacaaaa
     8401 caggtgcggc gtatcccttg ggatgcgccg cacctgtttt gttaaaaagg caaccaccga
     8461 ctgtgtcggc ggccttgact gcacaagaaa acttttggat ttctctccct ggatatccgc
     8521 ccaaaagcgc aaaaagagcc gccgcggaca caatgtccgc ggcggcgtgg tgacccagcg
     8581 gggattcgaa ccccggaccc actgcttaaa aggcagttgc tctgcctgct gagctactga
     8641 gtcagatcgg cgggccggcg atgccagccc gcattggctg ggatggcgcg gctcgaacgc
     8701 gcgaatgcag gagtcaaagt cctgtgcctt accacttggc gacatcccaa agtggaaaag
     8761 acgcggagga ccgggagtta ttcccgatcc cccgatcaat ggggtgggat aaggggctcg
     8821 aaccctcgac acccggaacc acaatccggt gctctgccaa ctgagctaat cccaccatat
     8881 acatttcaga gtttttgttt tgacgctggc gcgccaggag ggactcgaac ccccggccta
     8941 ctgcttagaa ggcagttgct ctatccggct gagctactgg cgcacattca agaaccctcg
     9001 gaaaatggag cgggtgaagg gaatcggacc ctcgtaccca gcttggaagg ctggtattct
     9061 accattgaat tacacccgca agctatgaga gcataccatt atccgcgccg gatgtcaaga
     9121 gttcctgaaa gaaaaacttt gaaaatccgg cgcggcatgg aaaaattttt atccgatcag
     9181 ccgtgataga gcttgtgcgt ttcgtatttc ggctcgcagg taaggttgat cttgagcttg
     9241 gagagcgtgt tctcatcggc gcgggcgaga atgacggtcg cgtgcgcctc gctcccggcg
     9301 agatcgttga gcgcctccat cgcccgcgag gcgaccggat cgctgacggc gcacaccgcg
     9361 agcgcgatga gcgcttcgtt cacgtgcagg cgcgggttct tattgccgag atgctccacc
     9421 ttgagatgct gcagcggccg gatgatctcc ggggagagga gcagcacgtc gtccgggata
     9481 ccggcgagcg ttttgagagc gttcagaatg cacgccgagc acgcgccgag cagcgagctt
     9541 gtcttgccgg tgacgatgcg cccgtcgggc agctccatcg ccatggcggg cttgtcgccg
     9601 ttgagctccg ccttttcgag cgccgggacg accggcgggc ggtccttgca cgtgatgccg
     9661 agccggttca tcaaaagctc gagcttcatg agagcgttct ggctggccat gcccttgcgc
     9721 acctcgcacg ccgtgtggta ccagcggcgg atgatctcct gctttgaggc ctcacagcac
     9781 gccgcatcgt cgcagaggca gtacccggcc atattcacgc ccatgtccgt ggggctcttg
     9841 tacgggctct cgccgagaat gcgcgtcaat atcgcgctga gcacggggaa gatctctaca
     9901 tcgcggttgt agttgaccgc cttctcgccg taagcctcga ggtggaacgg gtcgatcatg
     9961 ttcacatcgt ccagatcggc ggttgccgct tcgtaggcaa ggttgaccgg gtggttgagc
    10021 ggaagattcc agatcgggaa cgtctcaaac ttcgcatacc cggcgcgctc gccgcgctgg
    10081 tgatgatgat agagctggga aagacatgtc gccattttgc cgctgccggg gccgggcgcg
    10141 gtcacgacga cgagcgggcg ggtcgtttcg ataaaatcgc tcttgccgaa gccgtcgggc
    10201 gatacgatca gcgggaggtt ggcggggtag tcggggatga tgtaatggcg gtagaccggc
    10261 acattgagcg cttcgagccg cttctggaac tgggacaccg tgtcctgttc acggcagtgc
    10321 gtgatgcaca cgccgccgac gtacagcccg caggcgcgga acgcgtcgat aaggcgcagc
    10381 gcctccatgt cgtaggtgat gccgagatcg ccgcggcgct tgttgcgctc gatatcgtct
    10441 gcggagattg ccacgatgac ctcgctttnn nnnnnnnnct ccatgagcat gcggatcttg
    10501 ctgtccggcc ggaagcccgg cagaacgcgc gaggcgtgga aatcgtcaaa gagcttgccg
    10561 ccgaactcca gatagagctt gccgccgaac ttattgatgc gctggcggat gcgctccgac
    10621 tggagccgga catactggtc gttgtcaaag ccgatctttc tcatatcttt ctccctctat
    10681 tttactcgcc gtaatgcacg tcgccgttga gatgcgagag tgccgtgagg tattttacca
    10741 tgcagtgaac ggtgctgctc cggatgacat agtgcgtgtc tccggcgctg atgtaaaggt
    10801 cctgcgggcc catgagcgat acgccggtga tctcgcttac cgggatctcc gccttcggca
    10861 gctccagtgt gccgcggaaa aggcgcatat cgccgcggcc gagcagcttt gtgccgctgc
    10921 cggacgcgac cgtatcgacg cgcacgtcgg tgtcggtgaa aatgggctta tcctccgcct
    10981 cgtcgcacag gcggacgatc tcgcccttct gccagcgcgt ccagtcgcgg aggttatcga
    11041 agggaatatc ctccccgacg agaaagcccg tcggcagata acggaacgaa aagccgcact
    11101 tccagcaccg gacggtatcg tcccggctct ggagcgtatg catcctgccg cacttcgggc
    11161 agaggaagag cagccgctcc aggtgctcgg cgagggcctt tccctcaaag cgcacggggt
    11221 ttttccgctg gcgctcgtag gcgtcctcgt gaagatcgga aacgatgtgc tcgttgatct
    11281 cctgcgggct cattgcgcgc agctcctcgg gggagtatac gcgcacggcc tcgccgtgca
    11341 tcttcccgcg gcggatggag tttcccgccc agcgggggct tgcgaagtat ccgcccgtga
    11401 gcttgtaggt gacgagcgac gcgccgctcg agcgcgcgag cttgccgatc gaggggagaa
    11461 actccgccgt cacgccgtcc caggtgcggt tgccctccgg gaaaacgcac acgttcatcc
    11521 cgcggcggag atagcgcatc atcgtgagaa cggtgctcgc ggcgctgccg ctcttctggc
    11581 gggggatggg ggactggagc catcccacga gcttgccgcc gagtcccgcg cggagcagat
    11641 gctcgcttgt gacaaaatag gtctgctccg ggaaactgca cgccacgagg atggggtccc
    11701 agttcgtgac atggttgctc acgagcagat a
//
LOCUS       DAWAAE010000056        11643 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2475_length_11643_cov_3.297894, whole genome shotgun sequence.
ACCESSION   DAWAAE010000056 DAWAAE010000000
VERSION     DAWAAE010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_11643_cov_3.297894"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2105 a         3676 c         3681 g         2181 t
ORIGIN      
        1 cccgtaaatt tatagacgag caggcacacg agcgtgagcg ctgtggagat cgtgtcgttg
       61 agcgcgtccg cgccggacat ctccatggcc gtcgagccga tcttccgccc gatccgccgg
      121 tagaataccg acatataaat tttcacgagg atggagagcg caagcaccgc gaccgccgcc
      181 cacgtgaact ccatcgcctc cgggtgcagc agcttgccgg cagcgccgcg cagcgcgtcg
      241 atcccggcat agaggatgag ccccgcgatg acgagcccca tcacatactc aatccgcccg
      301 tggccgaacg gatgctcgag atcgggcttt ttccccgaca ggcggaagcc gacgagcgtg
      361 agcgcgccac tgccgacgtc ggagaggttg ttcattgcgt ccgccgtgat cgagaccgac
      421 ccggcgagcg cgccgacgac gaatttggcc gccgccagca ggatattgag cgtgatggcg
      481 accgccgagc aaagcatgcc gtaagcggtg cggacgcggg gatcgtccgt acggcggctg
      541 tttcggatga acagccgcgc aagaagctcg atcaaagcgc gcttcctcct ttgttatttc
      601 gatgcgccga cggtcgggtc aaggatccac gagccgccgc cgaacgtcca tgtcatctgc
      661 ggcacgaagc gcagcgtact gtcgatcttc ttttcggtga agagcggcgc gtcgtcgctg
      721 taggtttcgg tctcgatgac gagccggtcg tagaccttga ggaaattatc catcgactgg
      781 ccgttgtcgg taccggtcgt gagtacgtcg tgcggcacac gcgcgctcag atggacgttt
      841 ttgtccttca tcgtcttgtc gatcgcttcg cgcacggcga tggagaaatt catgcagaac
      901 gcctcgcggt tgagactgga ggcaatttcg cgcgtgtaca tgacctcggc gaacggatgc
      961 tccacacggg agaggatgat ctcatccacg cccatcgcca tgagatcggt gcagagctct
     1021 tcgatatacg tgcgcacatc gcggttccag agatccaccc agccgccgga ctcgtccaca
     1081 tagggcgcgc cggtgcggtc gcgcagcgca acgtccgggt tggccgaggc gagcagcgtg
     1141 tccacggcgc agcagatctc cgccgtgatg gtccatccct gcgccttgag ctcgctcaga
     1201 tacgccgtcg ggtcccaggt gctgttcgcg gcgttggacg acgccgtcgc cacgccggac
     1261 atccatgcga gcttgccgct ttcgtccttc atttccagca caaggccctt cacgccgctg
     1321 cgttcggctt ccttgaccgc cgtttccagc ccgttggcat tcaccttgga gaacgggaca
     1381 tagtatccgg tcagatactg cagccccgcg ctgcttgcga gcgaaacgtt ggtataatcc
     1441 ggctcggcga cctgaatggc ggccgaagcg tcgccggtat agggcacggg ggcggaaatg
     1501 ccctccacgg tgtaaccctt cccatcctcc tgcagcaccg gcacgaccat ctccacgccg
     1561 tcgcggtggt agacaacgta ttccggcagc agcgagaacg cgagcagcag cccaaggagc
     1621 acgagcacga ggatcgtgag cagcaccgga gagccgctgc gccccgcgcc gcggcggccg
     1681 cggtatacgc ggtggccctt aacgatcatg gttttcgagc gcggagatca tattcacgcg
     1741 gtcagcatgg cgtccgccga gaaactccgt cgtgagaaag agatcgacca tgcgcttggc
     1801 aagctcgggg cccgtgacac gcccgccgag cgcgcacatg ttcgcgttgt tgtgcgcgcg
     1861 gcacatctcc gcgctgaagc agtcggacag cagcgcgcag cggatgccgg ggatcttgtt
     1921 cgccgcgagc gacatgccga tgcccgtgcc gcagacaagg ataccctttt cgtacttccc
     1981 ttccgcaacg ccgcgcgcgg cgcgctcggc gtattcgggg tagttgcagc tcgcttcgga
     2041 ataggtgccg atatcctcaa actcgacgcc cttttccttc aggtgcgcca tcagctcctg
     2101 cttgagggcg tagccgccgt ggtcagatgc aataacgatc atgatgttta tctcctttgt
     2161 ttattccagc gatagaaatg tgggctttct ctgttcaaac accgtgtagt accgaaagcc
     2221 gatcgtccgc agcagctccg cgccctcgcg gatgccgacg gaggcgtcct ccggcgtgtg
     2281 ggcgtcgctg ccgagcgtga ggatctcgcc gccgagatcg tgatagagtg cgagcgccga
     2341 ggcgttcggg tagctcgtgt ggccggtgcg caggccggat acgttgatct caatgccgcg
     2401 tccctgcgcg atgagcgctt cgtataccgc ggcgagcttg tcgcggtatg cctccgccgt
     2461 gatctcggcg gaaaagccgt cgcggtgcat ataccgggaa gtatacccga tgtgcgccat
     2521 ggtgtcaaag tacggaaact ccgtcagctc caggagctcc gcgagatact cccggttgag
     2581 ctgcgcgcac tgctcctcgg acgtgtacgg atactcatag aaatccggct ttccgcgcag
     2641 attgtgcagc gagccgagaa caaagtccag ccccggttcg agcgcggctt ccttcgccgc
     2701 ggcagggtca tggttcgcct cgccgagctc aatgccgcag cggacggtga taccctccac
     2761 agggtttgcc cgcacatccg cccatgccgc gcgcaggatg tccttccgcg cctcccagcg
     2821 cagatcgccg agattgccga agtccatgtc gatatggtcg gtgaagcaca cggcgctcat
     2881 gccgtggtct cgcgccgccg ctgccatttc gcgcagcggc gtgtgcgaat ccggcgagca
     2941 ttggcaatga atgtgctgat cggtatagat catttttctt tctccttttc cggcgcgcga
     3001 agcgcgcggg caagcgcggc gcgtcccgcc gcgagccggt cgttccatat ggctgcgtcc
     3061 ggcgtttccg ccgtaagcgc ctcttcgagc gcggcggcga tatctccgca ttcttccacg
     3121 gtgcggtacg gtatgccgca ttccttcgca aacgcggcga tgcgtccgtc accgtcgatg
     3181 ccgaacgccg gtgttccggc aagatacgag agtgtgagcg cgtgcagccg catcgatacc
     3241 gtgagccgtg ccgaggcgaa aaggcgcgtg agcgccgcgg tatcctccgg cgcggcggcg
     3301 gcttccccgc cggtctccga gaccagcgcg cggcagagcg gcagatcctg ccggacgttc
     3361 atggcgatat accgcagctt ccagccgcgc tttgcggcga tctccgcggc ggcgcgggca
     3421 tattccgccg gaaacggcgg cgcgccgcgg ccccgccggg ggcagacgaa aaggacgttc
     3481 tcccgcggcg cggcggacac ggatgtttct gccgcgccgt ccgcgccgag aatgagctcc
     3541 ggagcgttta tgccgtgctt ccttgccgcg gcaaggcttt ccctctcccg aaagacgatc
     3601 gtatcggcgc attcgttcag cgccgccgtg catttggccc agccgcgcgg cgagagcggg
     3661 ccaagccccg cgccgtacag gagcgtgcgg cagccgcttt gctttccctt tcgcagcagc
     3721 gcgagataat agacaagcga ccggcgggac gtcgcgtcct gcagcagatt cccgccgccg
     3781 aagacgagcg cgcgggcgct cttcattctt cggcggatcg ccggaacgtt cagataatac
     3841 aaactgtcaa catcaaaccg gcgcgccgtt tcctttggcc ggtgcgagag caccgtgacc
     3901 gcgcattccg gcgcggcggc gcgcccatcg cgcagaagat tttcgagcat cagctcatcg
     3961 ccgcggttgc cgcatccgta ggctccggcg atcacgagct cccgccgggg cgagagaaca
     4021 gcgcggcaga tctctttttc gcgcagcgcc atggcggaga gggaaaattt ctttgccgtc
     4081 cggtcgcgga gcgctgtgcc gagcttttcg cgcagcgccg gatcgcgcga gagcatcaaa
     4141 atatccgatg caagcgcggc ggtatcgccg ggggcgatga gaaggccgtg aacgccgttt
     4201 tccacaagct ccggcacgcc gccgacagcg gtcgcaatga ccggcacgcg gtattttgcc
     4261 gcgtcggtga gcgcgtaggg gaacgtctcc gaaagggacg tgagaaggca gatatccagg
     4321 ctcgcgtaga gagcttccgt atcatcgagc cagccggtga aatggacgcg gtcggaaacg
     4381 ccgagctctt ttgccagcgt gcggagcatt tcctcctccg cgcccgctcc ggcgagaagg
     4441 agacggagct gcggctcctc ctttgcagca gcgtcaaagc cgcgcagcat cgtggaaagg
     4501 tcttttaccg catcaaaccg cgcggcagtt cccgcgagaa tatcgcccgg cgcggcgggg
     4561 atgccgtgca cggcaagaaa atcctcccgg cgcatttttg agcgcggcgc atccatatcc
     4621 gcgccgttat aaacggaaaa gagcctcccg cgccggaaat tccgggcggc atatttctcg
     4681 cgcattgcgc ccgacacgca cacgagtgcg tccattttcc gaagcgccag agcgttgagc
     4741 gtgccgtaaa caagccgggc agcgggccgg tgcagatagt cgagccggtg gtcgctgtgg
     4801 accgtcgata cgaccggcac gccgagagac gggcggagca gcgccgccgt cagattggcg
     4861 cggctgccgt ggcagcggac gagatcgaac ccgccggagc ggatgtatcg cttcgccgcg
     4921 cgaagctgcg caaaaaaacc gccggtgagc gtttcgcagg ggatgccgcg ctcgcgcgcg
     4981 tcgtcggcca gcacgccgcc gcccagacac agaagctgcg cctggccggt tttgttcagc
     5041 tcgccgagca gccggagcac atgggtgcgc gcgcccccgg cgtcgccgcc gctgatgaga
     5101 tgtacggttt tcacggcggt cctcccatga ccgcggacaa attccgcagg ctgttatctt
     5161 gccccgcagg ggcggactgt ggtatactta cattgacttg atattatacc ccatttccga
     5221 aggagtggca atacatgacc caaatattcc ttgtccgcca cggacagtcc gagggtaatc
     5281 tcttccgccg cgtacagggg cagaccgatg tggagctcac cgccgacggc aaggcgcagc
     5341 ttccgtatct cacgaagcgc tttgccgaaa cgccgctcgc cgccgtatat accagccctc
     5401 tccaccgcgc ggaggagacc gccgccgcca tcgttggcgg gcgggacatc cccgtttttg
     5461 cggacaagcg actcattgaa atgtgcttcg gcgcgtggga aatgcggccc tggggcgagg
     5521 tgaatctcga atcgccggag ctaaagcgcg cgtttctcca cgatcccgac cgctggcatg
     5581 tgccgggcag cgaggcgcac accgcggtgc aaaagcgcat gctagccgcc atgacggaca
     5641 ttgcccgcgc gaacgacgga aaaaccgtcg ccgtcgcgtg ccacggcatg gcgatccgcg
     5701 cttttttggc gcgcattctc ggcgtgccga gcgagcgcat cttcgaggac gtcccgctcg
     5761 tgaacaacac gtccgtcacg ctgctgcgct ttgaaaacga cacgttctcc gtcctctatg
     5821 tgaatgacac gacgcatctc cccgcgccgc cgtatacgcc cttccgggaa aacggaaagg
     5881 cgggcggccc gcgctgctat gatctgcgct accgcccgtt cgatctcgat gccgggcagg
     5941 agcgctacat tgcctgctac cgcgacgcct ggcgcggcgc gcacggcacg accgaggggt
     6001 tcaatgcctc cgcgtgctgg ctcagtgccc agctccacag cgccgaagcg ccggggtgca
     6061 tcaccgaggc gctggttgaa aatgaatttg ccgggatcct cacgctcgac gagcgccggg
     6121 gccgcgagcg cggcatcggc tggatcgcgt ttttgtacgt tgtgccggag ctgcgcggcc
     6181 accactgcgg cattcagctc gtcggcaccg cggcgatgcg cttccgccag cttgggcggc
     6241 gggccatgcg tctcaccgtc gcgccggaaa accccgcgct gggcttttac gaaaaggcgg
     6301 gctttgtgcg cgtcggcacg gaggcgggcg ctctcggcga cctcttggtg atggaatgga
     6361 ctttgtaacg catttttacg ataatcccgc ttaccctaac aatacctaca ctgttgcgca
     6421 tccgacgttt gacgatcatt cccgcatcat cgccgtatcg gacatccacg ggaatcttcc
     6481 gtattttcgc ggtcttgtgg aaaagctcca tctgcgcgcg gacgattcgc tcgtgctgct
     6541 cggcgatctg gtggaaaagg ggccggagag tctcgccacg ctccggtacg ccatggcgct
     6601 gcgggaaaag tgccgggtgt acccggtgct cggcaactgc gatttctggc atctctgggt
     6661 ggacgggtgc gacatggagt gggacgtgcg gacgctcgcc catctcctgc gccagaaggc
     6721 gacggcgcgc agcggcctca ttctcgaaat gtgcgccgag ctcggcgaaa cgctctccgc
     6781 ggacaccgat ctcgccgcgc tcaagccgcg gctgcgcgag gcgttcgcgc cggagtttga
     6841 ctatctccgg gccatgccgt tcgcgctcga gagcgacaaa tacatcttcg tccacggcgg
     6901 cattccccac ggcgaaacgc tcgagagcgc cgggccgtgg cgctgcatga agatcaacag
     6961 cttttacgcc gcccgcccgc actttaaaaa atgggtcatc accggccaca cgccggtgtg
     7021 tctctatggg acaaacacca tctccgccgt gccggtcgtc gatccggcgt gccgcgttgc
     7081 gagcatcgac ggcgggtgcg tcctcaagga cgacgggcag ctcaacgcgc tcattctccg
     7141 ccgcgggaaa ttcacgagcg agtggtacga cccgttcccg ctcggccgcg cgctcgacgc
     7201 gcagaaaaaa agcgcgcgct cggcctacat ccgctggggc gataacgccg tggagcccat
     7261 tgagctcggg cgcgaatggt gccgcatccg gcacatccgc acggggtacg tcatggacgt
     7321 gccgacggac tttctctatg aatccaaagg cgtgctgcgc gtgaacgacg tgaccgacta
     7381 ccgccccgcc atcgcgccgg gcgaaactct cagcatcgtg cgcgagaccg accgcggata
     7441 ctggatcaag aaaaacggcg tttccggctg gtattccggg cgcatcgagc gcctgtaagc
     7501 cctatacgaa aggagtctat atgtatcttt ccgattatct tgtatttatt ctcgccctgc
     7561 tcgcgtgcat ggtgttttcg atgatcgcag gcggcaaggt gaagagcgcg tactcggcgt
     7621 acagccgcgt gccctgccgc agcgggcttt ccggccgcga gactgccgag cggctgctgc
     7681 gcatgaacgg cgtatatgac atcgctctcg gccgcgtttc cggcgagctg accgaccact
     7741 atgccccgaa ccagagcatc gtaaatctct cgacgggcac cgcggacagc cgctccgtcg
     7801 ccgccgcagc ggtcgccgcg catgagatcg gccatgtgat gcagaacaag gagggctacg
     7861 gcccctaccg cgtgcgcacg gcgctcgtgc cggtcgtgaa tttcggctcg cggctcgcca
     7921 tgccgctcgt gctcatcggc atcctgctcg acacctacgt ccgcacggcg aaccccgaca
     7981 cgggcttcta tgtcgccatg gccggcgtcg tgctctacgg cggatcgttc ctcttctcgc
     8041 tcgtcacgct gcccgtggag ctggacgcct cccgccgcgc gggggaaatg ctcctcgccg
     8101 cgggcgtgat cgcggaggac gagctgccgg cggccaaaaa agtcctctcc gccgcggcgc
     8161 tcacctatct cgcgtcgctg cttacctcgt tcgtgtattt cctccgcttt cttctgtacg
     8221 tgctctccgt tttcggcaag cgcgaccggc agtgatccaa acagcagatc gcccctcttc
     8281 caaacggaag aggggcggtt tttttgcccg cggtttcgtt ccgggcacag tttcgatctt
     8341 ctgtaggggc ggggctctgc tccgcccgtc gagagtaggg gcggggctct gctccgcccg
     8401 ccgaaacatg tctgccctcg tcagaccgaa ccaacaaaaa gagcctccct tgtataaaga
     8461 gaggctcttt ccttatgcat tctcgtcgag atgccgctgg atgcggccta cgatgagatc
     8521 aagcgccacg gggtttttgc cgccctgcgg cacgatgatg tcggcgtttt tcttcgacgg
     8581 ctccacatac ttttcgtgca tgggcttgac ggtgttctgc cattgctcca caacgctgcg
     8641 caccgtgcgg ccgcgctttt cggtgtcgcg cagcacacgt cgggcaagac gcacgtcggc
     8701 gtcggcatcc acgaacacgc ggatatccat gagatcggag agtgcaggat cagcgaagat
     8761 cagtatgccc tcgaccagga tgaccgggcg cggctcgatc tcgatggtct cgccgctgcg
     8821 gttatgcacc ttgaagtcat acactgggca gtggaccgag cgtccctgct tcagaaggcg
     8881 cagatgctcg atcatcattt cggtctcgaa cgcctcaggg cagtcgtagt tgagtctggt
     8941 gcgttcctcg taggtgagat cgtcgtgcgc gcggtagtaa ttgtcgtggt ggacgataca
     9001 caccttgtcg ccgaagcgcc ggatgagatt ggtcgtgagc gtcgtctttc cgccgccgga
     9061 gccgccggca atgccgacga ccagaatgtc cggcagcgcg gcgcgctttt cgttatccgc
     9121 catcagcgcg tacccttgtc gtaggggatg ccctcggcct tcggggcgcg ggagctcttg
     9181 gaggtgaacg cgagcacgat gagcgtgatg agatacggca gcagccggta gaagtgcccg
     9241 ttcaggccga tggtgttcag ccagtaaatg ccgtcgccgt tgatgtccag cgtggagtac
     9301 gacgccgcga tgcacttgaa gaggccgaac agcagcgcgc cgcccgcaac gttcagcggc
     9361 ttccagttgc cgaagatcat aaccgcgagc gcgaggaagc cgaagcccgc cacgtcgccg
     9421 ttcgagctgc agttggccgt cgtcatggca taaacaaagc cgcccatgcc cgcgagcgcg
     9481 ccggagatcg tcacaccggc gtagcgcatt ttcaccacgt tgatgccgag cgatgccgcc
     9541 gcctgcgggt tttcgccgca cgcgcgcagc cggagaccaa agcgcgtttt atagagaatg
     9601 acggagagga tgacgaagat cagaatgcag atatacgttg caaaatagac cttgtgcaga
     9661 aacgtctcgc cgaggaagcc gatatccgcg ccgcggtcaa agccgagcgt cgttttcttg
     9721 atcataaacc agctcgcgct gtcgccggag agcatctgca gcgtgttctg gttggcgatg
     9781 acgcggatga ggaacagcac cagcgcggga gcaagcatgt tcagcgccgt gccgccgatg
     9841 gtctggtcgg ccttgaggtt gatcgccgcg aacgccagca gcagcgagaa caccgcgccg
     9901 agcgccgccg caacgagcat cgtcaggaac atatagccct gcagcgccat ccagtcgccc
     9961 gcggccttgg ccgcgtcaaa gcagccggtg gcctgcagaa tgcgcacgaa cagtacgccg
    10021 atgaacgcgc cgaagatcat aatgccttca agcgcgaggt tgatgatgcc gctgcgctcg
    10081 gcaaaaatgc ccgcgagcgc cacgatcatc agggggatcg cataaatgag tgtctgccga
    10141 atgagaaaag acattactcc tgcgcctcct ttccttccgc cgccgggagg actccggcgc
    10201 cggtctcgtc ctgcggtact gccggttccg ccgtcgattc agcggtctcc gtgtgtgccg
    10261 gttttttctt tttccgtccg ctgatccaca tcttgatgac cagcgagaac gccgccatat
    10321 acacgatcac ggcgatgata acgtcggtga tgtattcgtt gtaggcggtc aggttcgtga
    10381 tctgcatacc ggcgatatcc agaatggaca taaagcagcc ggtgaagatc acggcgatgg
    10441 ggttgttcac ggcgagcagc gccacggcga tgccgttgaa gcccgcggcg ggaagcgtct
    10501 ggtaggtgtt ccagtagaac tccgtgttgc ccgcaaggta gtagagggaa ccggcggcgc
    10561 cggcgagcgc gcccgcgatc gccatggaga gaacgatgtt gcgcttgtcg tggataccgg
    10621 cgtagcgcgc cgcgtggcgg ttggcgccgc aggcgcgcag ctcgtagccg aatgtcgttt
    10681 ttgtgagaac gatgtaaatg acgatcgcca gagcaatggc gatcagaata ccgccgctga
    10741 cctgcgagcc ggggaagagc ttgtcgagtc cgaacttgga ggtggcaacg ttgttgaacg
    10801 tcgtcttgta aatatagccg gacttcgtgt tctccaccat gtttttgaga ttgctgatat
    10861 caaagagcca tgtgacgatg ttcgccgcga tccagttggt cataatgcag gcgatgacct
    10921 cgttgatgtt cagcagcgct ttgagcaggc cggggatcga gccccagagc gcgcccgccg
    10981 ccatgccgcc gaggaacgcc agcagccaga tgatcacggg ggaaacctgc tcggacggaa
    11041 tgctcagcgc aatgaagagc gtggcgcacg ttccggcgag atactgtccc ggcgcgccga
    11101 tgttgaaaag accggtcttg aacgccgtcg ccaccgagag tcccgtgagg atcagcggca
    11161 tggcgcggaa gagcatgttg cccatgctcg cggggttaaa gccgaacgtc agtgcgccgg
    11221 tggcgtcacg cccggtgctg aaaaggccga agaatacgag ccggatgccc tcccatgcgc
    11281 tctggaagcc gagggacttg ttcgtgcagc cgacgatcag aatgagcagc gtaccggcca
    11341 gcaggccgat gaggatggat agcagtgacg cgatgaccga gcgcacgctg tcctttttcc
    11401 agaaggaggt tttttcttcg ttcatttctt tccctccgta tcctgccgct tcgcgccggc
    11461 catataaagg ccgagctctt ccacggaaac tttcttcgga tcgaattcgc cgacgatctt
    11521 gccttcatac atcacgagga tgcggtcgga gaggttcatg acctcgtcaa gctcgagcga
    11581 tacgagcagg acggctttcc cctcgtcgcg ctcgtggacg agctggcggt ggatgtattc
    11641 gat
//
LOCUS       DAWAAE010000057        11610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2487_length_11610_cov_3.425963, whole genome shotgun sequence.
ACCESSION   DAWAAE010000057 DAWAAE010000000
VERSION     DAWAAE010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_11610_cov_3.425963"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3328..3337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2306 a         3405 c         3445 g         2444 t
ORIGIN      
        1 cagttataat cgcaggaaaa atccgggctc gcaatgtact taccaagttc tgtacacaga
       61 tgaaaatgtc ttgtttggac gcagaattca caaataggct catccatgct cgttaaggtc
      121 tctgtcggtc ttccgcagaa tatgcatttc atttctgttc ctccttttct tccttacagt
      181 ataccctaat tttcgggtat attcaacctt tttttcctaa ctataatttg attagtgaat
      241 taattatggt gggaggatat gcggttgatc acctttgacc ccttatggga gacaatgagg
      301 aaaaaaggcg taacgacata cacgctaata accaggtatt ctttcagcaa gggaacactg
      361 gacgccttaa aacacaaccg cagtattacg cttgccacgc tcaacgacat ctgttctatt
      421 cttgactgta ccgtagaaga tgtcatttta tatattccgg ataaaaaaac cgagatctga
      481 accaacgccg aaaaagctcc ccgccgccaa gcgaacgttt ggcagcgggg agcttacata
      541 ttttctttta ttctttattt tccgcctttg ctttaatgcg ccggaggggg gtggccggga
      601 gcttcagacg gctgaggctc cagttccgct cggcggcata gcccttggcc atggcttcga
      661 gcgtctcgtt gaccattttg tgcaggctcg gctcctggtg cacggcgcgg gcaaagcgca
      721 gcgtttcacg cagcatccgc gcagagagat cgctgagcgt tacggcgccg gtggccgtga
      781 gcgtgccgaa aaactcctcg atgaattcct gccgctcggc catgctcatg ccgacgagaa
      841 cttcctccat cgcgcggtcg aacgcccgcg ccgacggcga caggttttcg cagcggacaa
      901 agctcgtggg cgtcgtctcc cacgtgaagc cgtcgtgcgc gccgagcaat gcggcggtgc
      961 ttttcacgac cgtgtagtcg cagtcattcc agaggagcgt gccgaccatg gcgttctggg
     1021 agaggatcgt tttgatgcgc ggcttgatgc gccggtagcc ctcggtctgt aaaattttct
     1081 cctgaaagcc ggggccgtcg ttattgtaga tgttcccgat gcgctcctgc acgctctcgg
     1141 gcaccatggc ggcagcgtac atggcgagat tgccgccctt ggaatggccg ccgacggcaa
     1201 tttttcccgg atagaccgag gccgcccatg tgagatactc gaccgcatcg cgctgcgcgg
     1261 ggatctcgtc catgacggtg agcaggcagt cctccttcca gccgacgatc gtatcgtccg
     1321 tgccgcggaa ggtcacatag tgctgtccgc ccggcagacg cagcgtgagc gcggagaact
     1381 gctcggtctt tttctcatcg tacacctggc ggaaaccggt gagcatcacc tcgcggaagc
     1441 gcaccgtttc cggcaggcgc ttgaccatcg gggcaatata cggtgagctg agcgcgccga
     1501 gcttttccgc gccgcccgcg gcaaaatatg cctctaccgc ctgcggcagc gggacgtaca
     1561 actcgttttc cggcacgatg ccggtaaaat cgagcgtgcc gatcttgcag tggatatagt
     1621 tatccacctc gttcggcggc gacaccgaaa acggcacatc gccgcgccag gtaaggtaat
     1681 cgagaatgtt cggcatagtg aaaccctctt tacaatccaa acagtattct tacgccggga
     1741 atcttccgga tgaccataca tgttataaca ccgagcgcga aatccgccgc agcgatgagc
     1801 gggagcagcg ccagcggcat gccgccgaag atctttcccg ccgcagcgaa cagcacgacc
     1861 ttataaaacg ggtcgagcag catgatctgg taggacattt ttccggtctg cgccagcgcc
     1921 cggcaatttt ccggaagggc gtttgccagc gccagcagca gtgccatcag cgcataggac
     1981 gtgacaagca gccgcagata cgcatcgatc ttcagctgca acgcaaagca gagcgcgctc
     2041 gccgccgtga tcgcggcggc aaggcacagc gcagggatac ggcgccggtc gaaaaattct
     2101 ttcagctttg gataataata ccgcagcacc atgccggaca ggaagaacgg aaggtgcttt
     2161 acggtccggc tgatctggaa gacctccgtc agaagagaaa gcccttttat ggagcagacg
     2221 gcagagaacg caccggcggc gatcatgccg atgatcgctt ttcgcggagc agtcttttca
     2281 tccttttccc agaaaagcgg cgcgatcaga agcatgagga agatcgcgta ggcaaaccag
     2341 taggcgcggc cgatgacgaa tgcatcgcaa agctggccgc cgatcgtgtc cgcgtgcgcg
     2401 aagtgccgga cgatcgcagt gctgaaaaat accttcaaaa acgcgaaaaa ccagaacggg
     2461 acgaggacac ggagcacctt tttcttgtag tacgggcgca gcggctgccg gtggcacaaa
     2521 aagccggaga tcgtgaaaaa gagcggcaca tgcacatgat agatcaggtc ctctgcgatc
     2581 ccggcgaaga gcggcagagg ctgccaggcg gtgtaatagg aaaggcagtg gcccagcacg
     2641 acaagaaacg ccgcaaacgc tttcagatag tccagattgg cacttgtccg tttatcaaag
     2701 tgcatggcgc tcctcccctc ctccgttatc gactcgattc gcacctgtaa acatatcaca
     2761 gttttcgccg tctggtcaag agggcagcag ccggtatctc tcgcggctgc cgatcagtcc
     2821 acccgtgtat agatcatgtc tccaatcgga ttgttgtctg tggccataat aataagacta
     2881 ttctccgtta tctttacggg gaacacgttt tctttgccat catgatacag cgtcaccgtt
     2941 tctccgtcat aggaataggt tccatttcct ccctgcacgc tcagttttaa tccgatcgga
     3001 ttctgcgcat agttttcata cgttccgtcc tctttaaaag aatagactaa ggtaatgcca
     3061 acaatgctcg atttccatgt tccgacgata gcaaggagtt tcagtttctc cagtctttcc
     3121 tccgcttctc tcagaatatc cgaattggac accatgcctt tttcttcttc cgagagcgct
     3181 tcgtactttt cacggacgtt ctggatctgc tcctttttct cctctgtcac ctcgccaatg
     3241 tctgcgatct cctgctcgac tgcgttgatc tccgccagac gcctcagttc ttccaatctt
     3301 ttttcggcct cctgcagcgt ttcgtagnnn nnnnnnnacc gctcttactt ctttgctctt
     3361 cccgcagccc gcaagaaggg aaactgccag aagaagcgct aaagcactgc tgatcctctt
     3421 tttcatttgt gtttctcctt tcacttagtc ttttcaatat accctgattt tcgggtatat
     3481 tcaacccttt tttctaaatt ataatttgtg tagggagttg ttttttgcag gaggaaatgc
     3541 ggttgatcac ctttgacccc ttatgggaaa caatgaaaaa gaagggcgtg acaacataca
     3601 cgctgataac caaatactcc ttcagcaagg ggacgctgga tgccatgaag cacaaccgga
     3661 gcattactct taccacgctc aacgacatat gctcgatcct cggctgctca gtggaggacg
     3721 tcattttata tattccggac gagaaatagc ggatattgaa tttcaaaatt caatacccgc
     3781 tatttctttc attaagcaag aaaaggagct gggatttctc ccagctcctt ttttgcgatt
     3841 tggaattatt cgtacagcgc cttgagcttg agcaggtgct cgtagcgggc cttggcgttc
     3901 tcttcgttct tctcgaagag ctcggtggcg cgctcgggga aggcggtggc gagacgcgcg
     3961 tagcgggcct cgttcttcag gaagtcctga tagccgccct tgggctcctt gctgtcgagc
     4021 gagaagggct tctccgccgc cgggttgtac cggaagaggt tccagtaacc gcagtcaacg
     4081 gccttcttca tctcaagctg gcagttggcc atgccgccct tgatggagtg catctcgcag
     4141 ggggagtagc cgatgatgag ggacgggccg tggtaggcct cggcctcctg gatagccgtc
     4201 atggcgatct cggcgagaga cttgctcttg atctccttgc cggccgccgc aaactgcgcg
     4261 acctggccga tgttggaagc cttggaggcc tgtccgccgg tgttggagta cacctcggtg
     4321 ttgaacacga acacgttgac gtcttcgccg gaggccagga cgtggtccag accgccgaag
     4381 ccgatgtcga acgcccagcc gtcgccgccg aagatccaga cggacttctt ggcgaggtag
     4441 tccttgccct cgaggacctt gcgggcgagc ttgcaggcgt cgcactcgca ggtgggcacg
     4501 ctcgggttgg cgttgatggc ggcgacgagc ttctcagccg cggcggagtt cttctcgccg
     4561 tcgttgacgg tgtcgaggaa ttccttgccg gcagcggcga tgtcctcatc gacgtaaggc
     4621 acggcgatca gttccttgac ggcctcgatg tacttctcgc gaagcacctt ctggccaagg
     4681 tacatgccgt agccgttctc ggcgttgtcc tcaaagaggg agttcgccca ggcaacgccc
     4741 ttgccgttct tatccatgca gtacggagaa gtggcagccg gtccgcccca gatggaggag
     4801 cagcccgtgg cgttggatac gtacatacgg ttgccgaaga gctgggtgac gaggcgggcg
     4861 tagctcgtct cggcgcagcc ggcgcaggag ccggagaact ccagatacgg cttgaggtac
     4921 tgggacttga tgacgttgtc ggtgccggcg acgtcggact tgacggtgac gtccttgacc
     4981 atgtagtcaa agaccttctg ctcgccgagc tggctctcct ggccgaccat cttgagggac
     5041 ttggtcgggc agacgctcac gcacacggag cagcccatgc agtcgagcgg gctgacggcc
     5101 agggcgtact tgtacttgcc cttggacatc ttgtgatcga ccatcttggt gccttcggga
     5161 gccgcggcgg cttcctcggc gttgagagtg tagggacgga tggtggcgtg cgggcagacg
     5221 taagcgcact ggttgcactg cgcgcaggtc tcggcagtcc actcgggaac ggtgacggaa
     5281 acgccgcgct tctcgtacgc agcggcgccc tgctggaagg ttccgtcggc gtaatcgacg
     5341 aaagcggaga ccggcagaga gtcgccgttc atgcggccca cgggctccat gatgttgcgg
     5401 accatcgcaa cggtcttttc ggggccttcg agatgctcaa cgtgagcttt ctcggtggcg
     5461 ttcgcccagt cggccggaac atcgatcttc acgagcgcgc cggcgccggc gtcgatggcg
     5521 tcccagttct tctggacaac gtccatgccc ttcttcatgt aggagtgctc ggcggcatcc
     5581 ttcatgtact tgacggcctc atccttgggc agcacgttcg ccagagcgaa gaacgcggcc
     5641 tgcaggatgg tgttggtgcg cttgcccatg ccgaccttga cggcgaggtc aatggcgttg
     5701 atggtgtaaa gctgcacgtt gttcttggcg atgtagcgct tcgcgtcggc gtggaggtgc
     5761 ttgttgagct cctcgaaatc ccactggcag ttgatgagga agatgccgcc gggcttcacg
     5821 tcctgcacca tcgggaagtt cttcacgatg taggacgggt tgtggcaggc gacgaagtcg
     5881 gccttgttga tgtagtacgg gctcttgatg ggcttgtcgc cgaagcgcag atggctgatg
     5941 gtgacgccgc cggtcttctt ggagtcatac tggaagtagg cctggacgta cttgtcggtg
     6001 tggtcgccga tgatcttgat ggagttcttg ttcgcgccga cggtaccgtc gccgccgaga
     6061 ccccagaact tgcactcgat ggtgccttcc gcggcggtgt tgggggtctc ctcacgggac
     6121 ttctcggtga gggagaggtg ggtaacgtcg tccacgatgc cgacggtaaa ctggctcttc
     6181 ggctcgtcct tcttgagctc gtcgtaaacg gcgaacacgg acgcgggagg cgtgtccttg
     6241 gaggagaggc cgtagcggcc gccgatgatc ttggcctgac ggccggaggt ggcgaacgcg
     6301 gtcacgacgt ccatgaacag aggctcgccc tgcgcgccgg gctccttggt gcggtcaagg
     6361 acggcgatct tcttggcggt ctcggggacg gcggcgacga gcttgtcggc gcggaacggg
     6421 cggaacaggc ggaccttgac aaggccgacc ttctcgccgt gagcggtcag gtagtcgatg
     6481 acttcctcgg ccacgtcgca gatggagccc atcgcaacga tgacgcggtc ggcatcggga
     6541 gcgccgtagt agttgaagag ctggtagtcg gtgccgagct tggcattgac cttcgccatg
     6601 ttctgctcaa cgatctcggg cagggcgttg tagtacttgt tggaagcttc gcggttctgg
     6661 aagaagatat cgccgttctc gtgggagccg cgcatcgtcg gatgctcggg gttgagagcg
     6721 cgctcgcgga acgccttgac ggcatcccag tcgagcatgt cggccagatc atcgtaatcc
     6781 cagacggcga ccttctggat ctcgtgagag gtgcggaagc cgtcgaagaa gttgatgaac
     6841 gggacgcggc cctggatggc ggacaggtgc gccaccgggg agaggtccat gatctcctga
     6901 atgttggtct ccgccagcat ggcgaagccg gtctgacggc aggcgtacac gtcgccgtgg
     6961 tcgccgaaaa tgctcagcgc gtgggacgcg accgcacggg cggaaacgtg gaacacgcag
     7021 ggcagcagct cgccggcgat cttgtacatg ttggggatca tcagcagcag gccctgagag
     7081 gcggtgtagg tggtggtcag cgcaccggcg ttgagagagc cgtgcacgct gccggcggcg
     7141 ccggcttcgg actcaagctc cgcaaccttg acggtggtgc cgaacacatt cttccgaccc
     7201 tcggcagacc actgatccac gtagtccgcc atggggctgg acggtgtgat ggggtagatt
     7261 gccgcgactt cggtaaatgc ataggacacg tgggcggcag cggtattgcc gtccatggac
     7321 ttaagctttc ttaccattcg attcatctcc tataattttt tgctcctgtt ccggaaatac
     7381 aacagggctt tccgaaacgt ctttgcgaac agagtcattt taccacgccg gcccgccgat
     7441 gtaaagagtc tgcgaagggg ttttttctct caattttgcc tgaatcgcac aggatttccc
     7501 ccgatttttg gtatgttttt cacgttctcg gcgacaagaa agtccttgta cttttcgaca
     7561 atctgtttta taattataaa tcgatttgtg gaaaggaaga cttgccatgg taaaacttga
     7621 caacgaaaga ggctccatct gcatcagctc cgaagtgttc acttggctgg caggggatgc
     7681 ggcgacgcgc tgcttcggcg tcaaaggcat ggccggacgc gccaaggaaa gcggtctggt
     7741 ccagctcctc cgtcgggagt ctatgaccaa gggcgtctcc gtcacgccca acgacgacgg
     7801 aagcattacg atcgaactcc acatcgtggt cgatcacggt gtgaacattg ccgcgctctg
     7861 cagcagcatt atgaacgaag tcagctacaa ggtctcctcc gctacggatg tttccgtccg
     7921 tcaggtggac gtctatgtag attccatgac gatggactga ttagccaagg aggaagcccc
     7981 gcagtgagaa atacaatcag cggtcaggta ttcgcggaaa tgatcttctg cgcctcggcc
     8041 gcgctggatg agaatcgcca gcaactcaac gagcttaacg ttttccccgt gccggacggc
     8101 gacaccggca cgaatatgtc tctgaccatg tccgccgcct cggccgctct gcacggccgt
     8161 acgccctccg ccgtcggcga cgccgccgat accgccgcct ccgcgctgct gcgcggcgcg
     8221 cgcggcaact ccggcgttat cctctccctg ctgttccgcg ggctcgccaa gagtctgcgc
     8281 ggcaaggccg agtgcagcgc cgcggactgg gccaaggcga tgtgcgacgg cgttgacgcc
     8341 gcctacaaag ccgttatgaa gcctgccgaa ggcacggtgc tcaccgtgtc ccgctgcgcc
     8401 tcggaagccg ccgttgagcg gagcaaggcc ggcgtgaccg atatcgaaga gctcctcacc
     8461 gacgctctcg ccgtggcgcg cgaaacgctc gccaagaccg tggacatgaa ccccgtgctc
     8521 aaaaaggcgg gtgtcgtgga tgccggcggt cagggctacg tcgtcattct caacgcgatg
     8581 ctgctctgcc tgcagggtaa gttcgtcgcc aaggctcccg cgacggagac caagacgagc
     8641 gcggaattct ccgcgatcaa cgacgaggag atcacgttca cctactgcac cgaattcatc
     8701 gtcgcgcgtg aaaaaaagcg caatccggag ctgctgcgca gctttctcga gagtctcggc
     8761 gactcgctcg tgctcgtgga ggacgacgat atcatcaagg tccacgtcca cacggacgcg
     8821 ccgggccgcg cgctgactga agcgctgcgc tacggcccgc tccagaccgt gaagatcgag
     8881 aacatgcgca accagcacac ctctctcgcc gacaaggaga tcgccgccgc gcctgccgcc
     8941 cccgcccagc cggaggccga ggccgagccg gagatcgccc ccgccgagaa ggaagtcggc
     9001 ttcgttgccg tgtgcgccgg agacggcatg gccgatctct tccgctcgct cggcgccgac
     9061 cggatcgtga ccggtggcca gacgatgaac ccctccacgg acgacattct ccgcgaggtg
     9121 aacaagacgc ccgcctccac cgtgttcgtg ttcccgaaca acaagaacat catcatggcg
     9181 gcggagcagt gcatcccgct cacggaaaag accgtgcgcg tcatcccgac ccgcaccgtg
     9241 ccgcagggcg tcgccgcgct ggcctacatg gatcccgacc agtcgcccga cgatctggag
     9301 gaatccttta aggagagcgc cgcgcgcgtt cacaccgcgc tcgtgaccta tgccgcgcgc
     9361 gattcggact acgacggcca catcatccac gcgggcgagt atctcgcgct gctcgacgga
     9421 aagctgctcg gcagctacgc cagtctcccg acgctcctca aggagctcaa ctgggcgttc
     9481 ggcgagctcg agccggaatt tctcaccgtg tactacggcc aggacgtgac cgagaccgat
     9541 gccaacggcg tcgccgagag tctcacctcc tgcttcccgg aggcggacgt ctcccttgtg
     9601 agcggcggtc agccggtata ttattatatg atcgcggcgg agtgaacgtt tttccctccc
     9661 cttccgtctt ataaaaagag acccgctccc gcgggtctct ttttccaaag aaagaaaagg
     9721 ataaaagacc tatggcaaaa cgctctgacc attccggcgg cacggcgctc aagacgcgcc
     9781 cgcgcgcggg gcacatcgtt ctctgggccg tgctcgcggc gctgcttctc ggcgcggtga
     9841 tcggcgtcgt ttcgctgctc ggccgcccgt ccggcacgga aacgccggag aataccgcag
     9901 cacccactgc cgcgccggac acctccggca cgccttcgga aacgccgctg cccgaagaac
     9961 tgcgcgaagc gcctctctcc cccgaagcgg agattgccga gcgcgccgcg gtgcgctcga
    10021 acggctttcc gatgctcgcc tgggcgccgg acggcttttc ggccgacagc cggggccgga
    10081 tacagtattc cggcgggaaa tatctcaccg gcatcgatgt ttccgagcac cagtacgaca
    10141 ttgactggga aaaggtcgcc gccgacggca ttgactttgc gatgatccgc gtcggctacc
    10201 gcggctcgac cgccggccag atctatacgg acgaatactt tgaaaagaac atcgccgggg
    10261 cgaccgccgc cgggctgaag gtcggcgttt acttctattc gcaggcgatc aacgccgagg
    10321 aagccgccga ggaagccgcc ttcgtgctca acgcgatccg cccgtacaag gtcacgttcc
    10381 cggtcgtgtt cgactgggag atcgtcggcg ggagcgacgc gcgcacatat accgtctcgc
    10441 gcgcggtgct ctgcgagagc gccaaaacgt tctgccgcgc cgtcgaggac gccgggtatg
    10501 acgcgatgat ctatttcacg cagtatctcg gctaccggaa atacatactg cgccagctct
    10561 ccgactacga attctggtac gccgaatacg aggcgaagcc gcgcattgta ttcaacttcg
    10621 acatgtggca gtattcgagc accggctcgg tgaacggcat cgacggcgac gtcgatctga
    10681 acatctattt ccggaaaaac tgaatgtttc ctccctccgc ccgcggaaaa gtgcgggacg
    10741 gagggagttt tttcttgaca ggcggcagcg ccgcgctata atagttgtac atacaacgat
    10801 gtacctgcaa ctttttattc catttattct attctccgga ggcgtctatg gacattacgg
    10861 aacggaaaat caccaagatc gcgcgcgagg cggaaaagct ggtgctgctc tcgctgcgcg
    10921 aggagggcgt cggcacggcg gagatcgatc tgatccacgc gctgcgccac catcccggct
    10981 gcacgcaggc agcgctcgcc gagcttttgc acgcggacaa ggccgccatt gcgcggcgga
    11041 cgaaaaacct tgaggcgaag gggtatcttg tccgccggga cgcgcccaac gaccggcgca
    11101 gccagctttt gtatcccacg gaaaaggcgg agagtctgaa gtcctccaaa gcggagatcg
    11161 aagcggcgtt ttatgaatac ctgacaagcg cgctcacaaa ggacgaggcc gccgcgttcg
    11221 ccgcatcgct ccataagctt tacacggtct ccaagaccga gagccgcgcg ggattcccgc
    11281 atttccgaag cgctgcaaag aaggcgtccg ccgatgaaga ataagaataa gagcttccgc
    11341 gcggacgcga tcggctccta tttccggctg gaatggctgc cgctgctctt cgtgacgctc
    11401 tcggggctcg tatacaacgt cggccttctg gccgggccgt ggtacgaggg ccggctcgcc
    11461 cagtgtctcg ccgatatcct cggcgggaat gcgagtgccg cgaacatgac tgcgctcgtg
    11521 ctcggctata ttctcgtcac gcttctcgtg cagacagcgc ggtttatcaa gcgcttttat
    11581 gtccgccgct ttgccaacaa catcaaccgc
//
LOCUS       DAWAAE010000058        11216 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2598_length_11216_cov_3.394051, whole genome shotgun sequence.
ACCESSION   DAWAAE010000058 DAWAAE010000000
VERSION     DAWAAE010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_11216_cov_3.394051"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4273..4282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6367..6376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10520..10529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10845..10854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2087 a         3537 c         3345 g         2207 t
ORIGIN      
        1 ggcgcgggca agccgtggga cctctcgcgc aaggagggcg gtctttatat tctgcccggc
       61 acggtgttcg gcttccacga agagaacgcc cgttttctct tccgcgatct gctgcacaat
      121 ctggaatatc tccgtcaggg cgacggcgac tatatcctcg tcagctccgg cacgctcgtg
      181 gcgaatatcg actatcagcc gatgatcacc aagcacgagc tggagggcaa gcccgtcacg
      241 ctgctctata agaaaatgca gccgggtgag aagcgcccgg ggcgctatct cacgctcgac
      301 agcgacaccg tcgccggcat ccggcgcgcg gacgagggcg aaaacctcct gcttgactgc
      361 ttcatcgtcg ataagtcgtt cctgctccgc atggcgcgcg actatgccgc gctcgactat
      421 atggattttc ttgacattct cgcccacgtt ctgccggagg tcgccgtcgg ctgctgcgct
      481 tttgacggct atgtcgcctt catggacggc atggaggact atttccgtgc cagtcttgat
      541 ctgctcgaca gctccgtccg ccgcgagctc ttccagtccg accggcccat ctgcaccaag
      601 gcgcacgaca cgccgcccgc gctctatgcc cccggctccg cggtgaaaaa ctccgttatg
      661 accgccggca gcatcattga gggcagcgtg gagaacagcg tgtgcttccg caacgtccgc
      721 atcgaaaagg gcgcggtcgt aaaaaactgc gtgctgatgg aaaagtgcgt cgtcaaggcg
      781 ggcgcgcggc tcgaaaacgt cgtgtgcgat aaaaacgtca ccgtttccga ggacaagatc
      841 gtctgcggca cggcggagca tccctgcgtg ctggcgaaag gcggcgttgt atgaaggaaa
      901 agctgcgcgt tctctttgcc gcgtttgaag ccgctccctt tatgaagacc ggcggtctcg
      961 gcgacgtggc ggcgtctctc cccggcgcgg tgcagtgccc gacgatcgag atgcgcgtcg
     1021 tgctgccgaa gctccggcag attcccgccg agtaccagaa gaagatgaag ttcctctgct
     1081 cgttctatgt cccgctcggc tggcgcagcc agtactgcgg gctcttcgag ctgcggcgcg
     1141 gcggcgtcac ttggtatttc ctcgataacg agtattactt cgggcgcgac ggcgcttacg
     1201 gctactttga cgacggcgag cgcatggcgt tcttcgccaa ggccatctgc gaatgctgcc
     1261 ggtatctgcc ggactttttc ccgaacattc tccactgcaa cgactggcac acggcgctcg
     1321 cgccggtgtt tctgcgcgag cagtaccgct acgcccccgg ctacagcgac atccgcaccg
     1381 tgttcacgat ccacaatctc aaatttcagg ggcagtataa cccccacgtc ctcagcgaca
     1441 ttctcggtct ggacggcgtg gccgcggcgc gcaatcagct tctgcgggac gacggaaagt
     1501 gcgtcaacta tcttgccggg gctgtcagct actccgaccg cgtcacgacc gtcagcccga
     1561 cgtatgccaa ggagatctgc acgccgtatt tcggcgaggg actgcaggat ctcttcctgc
     1621 ggcggcacga cattctctcc ggcatcttaa acggcatcga caccgtttcc tacgatcccg
     1681 cgaaggacaa ggcgctcgtg caaaactata ccgaccccga cggcaaagca gcgaacaaga
     1741 aggcgctgca ggaggagctc ggcctcaccg tcgatagtga agttcccctc tgcgtgtgcg
     1801 tcagccgttt gacggagcag aagggcttcg atcttctcaa cgccgtggcg gaggaaatgc
     1861 tctcctgcgc gcagatcgcc gtgctcggca cgggcgaccg ggcgtatgag gactactacc
     1921 gcgcgctgga ggcccggcac cccggccggt tcagcgccca gatccgcttt tccgagccgc
     1981 tttcccgccg gatgtacgcc ggagcggaca ttttcctcat gccctcgcgc tttgagccgt
     2041 gcggcctgag ccagatgatc tccatgcgct acggcaccgt gcccgtcgtg cggcagaccg
     2101 gcggacttgc cgacagcgtt gagccctaca accgctacac cggcgagggc accggtttcg
     2161 gtttcctgaa tttcaacgcc cacgagctgc tcttcaccac acaggaggcg tgccgcctgt
     2221 acaccgaaag ccccgtcgcg tggggtaatc tcgccgcgca gggcatggcg aaggatttct
     2281 cctggcgcgc gagcgcgctc gaataccgca agctgtaccg aggtcttttc acatgatcgt
     2341 acacgaatcc agaaacagcg ccttccgccg tccgttcggc gccgccgaga ccggcacgga
     2401 ggtatatctc gccgcggaaa aggcgggcgc ggcggacatg acgctccggc tgcacccgtt
     2461 cacgggcgag gatacactcc tccccatggc atccgagggc ggcgggcggt tttccgtcac
     2521 ggtgacgctg cccgaaacgc cgtgcgtgct ctggtactcg ttccgtaccg gagacgacga
     2581 gacgtgcgcc tggcagatca ccgtatacaa aaaagccgcc gtccccgact ggtggaagga
     2641 cggcgtcgtc tatcagatct ttccggaccg ctttgcccgt gagcgcggct ggacgccgcg
     2701 cgagccggag gcgcgccgcg gcacgcaccg ctttctcgtg cgcgactggg acacgccggt
     2761 gttctatccc cgcgcggaaa acggggacgt atcctcgtgg ccgttctggg gcggaacgct
     2821 ccggggcatt gaggaaaagc tgccgtatct cgcctcgctc ggcgtcacgg tcctgtacct
     2881 gaacccgatc tttgaggcgg caagcaacca ccggtacgac acggcggact atacccgcgt
     2941 cgatccgctg ctcggcagga aaaaggattt tgaatcgctc tgcgaagccg cacggaagcg
     3001 cggcatccgc atcgtgctcg acggcgtatt caaccacacc ggcgcggaca gcgtgtactt
     3061 tgacaagttc ggaaactacg gcacggcgga aaaataccgc tcctggttcc gcttcggcga
     3121 tcagtaccgc cacggctacg aatgctggtg ggacgtgccc gatctcccga acgtggagga
     3181 gaacgatccc tcgttccggg aatttctctg cggcgaggac ggcgtggtgc gccgctggct
     3241 gcggcgcggc gcttccggct ggcggctgga cgttgccgac gagctgccgg acagcctcat
     3301 ccgcgacatc cgcgcggcga tgaaggaaga gaaagaagat tctctccttc tcggcgaggt
     3361 ctgggaggac gcctccaaca aggtgagcta cggcgcgctg cgcgagtatt tcctcggcag
     3421 cgagctggac gcgacgatgc actatccgtt ccgcaccgca gtactcgact tcctgcaggg
     3481 caagatccgt gcgcaggccg cctgcgacgc gttctggacg atccaggagc attatccgaa
     3541 ggagaatctc tatgccgcgc tcaatctcat cggcagtcac gaccgcgccc gcgtgctcac
     3601 ggtgctcggc ggcgatgtga acgcgctgaa aatggcgatg ttcctccagt ttgctctgcc
     3661 gggcgtgccg agcatttact acggcgacga ggccggcatg accggcggca cggaccccta
     3721 caaccgcggg agcttcccgt ggggcaaggg caacgccccg ctcacggatt ttgtccggag
     3781 tctcaccgcc atgtatcagg aaacgccgct gctgcggcgg ggtgagtgcg aaatggtgtt
     3841 cttcggcgag aacgtgctcg gctgccgccg gtttgacgaa agcggctccg tgctcgcgct
     3901 tgtcaaccgc ggagaagaag ccgttgaatg cttcggcgtc accgtaccgg gaagggggta
     3961 tattctggaa tgaagcacta catcatttcc aaattccgcc ccggaacgcc gtggcgggag
     4021 catctgccgg agatcgaggc gatcttcgcc cggacgctca cgatcgaggg cgtccacggc
     4081 gtggacatat tcccctcctg ctccgagcgg ccgaaccggt ttgatcttat gatctgcatg
     4141 gacatggacg ccgccgcgct cccgctctac gacgcgagcg agccgcaccg cacttggaaa
     4201 gaaacattcg gccccctgct cgaaggcaag accatttttg accacgaata agcagcaaaa
     4261 agctccctca tcnnnnnnnn nngctttttg ctgcttatat actttctttc tttttgactc
     4321 tgaagagttt tccgtagtcg aaactccagc tctccttttt gaggccgaac aggaaccacg
     4381 caaaatactt cacaaacatc acgatgaccg gcgtgaggag aaggatgccg aggagctggc
     4441 agaggctcac gatatatacg ttcgtcacgc cgatgtaccc catcgctttt tttaccagca
     4501 gctccggaag attatcgaag aggtagaaga gcatcgagtt cacgccgata taggagagaa
     4561 ccccttttct cttcacgttt ttcagcgcgc cgtacagcgc gagacacagg gcaaagccgc
     4621 cgatcatcac cggcacaaag ggtgtcagcc gtatcacgcg gtaatcgatg caatatttct
     4681 ccggggaatc cgaaaacagg agcgtcagcc cggcggcagc ggcaatacca acagctgcga
     4741 gaatcagtgt ctttttcccg gtgatctcca cgttcttttc ccggacgtac tgccccagat
     4801 acatgaagag cacgcatggg aatgccacat cgatatacca cggcagggag aaaccggtaa
     4861 atttgttgag atacgcaaac ccggcggacg cagcgagaac aacgggaaat tcgatcctcc
     4921 ggtcgtgaac gtactttcgc agcgcataaa agaggacgct cgccacgaac gccgccgtca
     4981 ggaaccactt cggcgcgacg agatcggtcc cccgcagctg aacgagcagg ccgagaatgt
     5041 tgtcccggca gagcacccag tccgcaccgc cgcgcagcag cggcacaaca atgccgagac
     5101 agagcacatg atagaggatg ccgtaagcga aaaacggcac catgagctgc cggaagcgct
     5161 ttttcacaaa ggcgccgaac gttccggcgg tgctgctgta gtagcccatg cagaagaaaa
     5221 acatcggcat gtggaacagg taaatgaaga tgcgcgccgc ctgcggtatc gcgtccgaat
     5281 ggccgaggaa caccagaagg atgcatatcc ccttggcgta atccgcccac aactctctct
     5341 ttttctctgc cataggttcc tcccttaata aaaagctttt cctgaatgtt ccgccggctt
     5401 catcgtggca ggatactttt cgcttttatc cgaagtattt ggcggcatcc tcggcggaga
     5461 cgccgaagaa ggcgatggcc tcgttctgca tccgcgcggc gcggccggag acgaagccgc
     5521 ggagattcgc catctgcgtg gcaaacaacg tggacgactt gccccagcgg gcgagatcgc
     5581 gcgcgctctc cggctcgacg atcgcgcgca gctcgtcaag cgttttctgc gcgttctcgt
     5641 ccgagaacgg cccgcgcagc atttcgccga gacgcgtcag gaacatatcg cggaactctt
     5701 cgttgtaacg cagcgccgag ggaatgatgc cgtggagctg gaagttgttg aagccgacgc
     5761 tcatcgagtc catgcccatc atcgtgagat ccatgtccgc gagaccgtaa atgtacttcc
     5821 cgttttcgta ctcggggctc gcgtagaagc ggacgttttc gtatacgtcg atatcgcccg
     5881 accagcactc gagaatcagc cagtcgatca tctccgggat gtccacatgc tcctggatgt
     5941 acgcccagcc ctccggcgtg ctcagcgagt tgtagcgcgc gtagttcaag atctcgctcc
     6001 acgcggtctg accctcaaac tcgcccgtct gcatgtcgac ggtgttggcg tccacgccct
     6061 tgtgcgaggc gaagtattcc gcgctgtggt gctcgcggat agcgtaaatg ccccagtact
     6121 cgccgttgag gaagagcgaa acataccggt aattctgcgc gggcacatcc gtatagtcca
     6181 tggcgatgcg ggcgaagaac tcgtcgcgca tgtaggaggt gtaggtgtcc tccacgctcg
     6241 cgcgcagcag cagcgaggaa aagtccgtca cgacgccgtc gccgaacacg tcataattga
     6301 gatccccgcc gtaccggccg cggaacttga gcgtgaacga tttcttttcg ctcacgcggc
     6361 ggctcgnnnn nnnnnncgtg cggccgtgca tgcggatgcc gcagtcgatg gagaactcgc
     6421 cgccgtcctc gtacatggcg acggtcgcct cgcgctccca cttctcgctc cacgcctgct
     6481 cgtggttgga gtaaatgccc tgcgcgccgg tgagattgtc cgggtcggtc acgagactga
     6541 cgacaggcag cgtgctgttc tcgcgcagga agtacgacca tgtcgccgct ttgcccggca
     6601 gcgcgtccgc cgggaagctc gcggcgcgga cgatcgtcgt ttccgtgatc gtgatgggcg
     6661 cggtataggc cggatcgtcc gccgtcgggt cgctgccgtc gagcgtatag tgcacgttct
     6721 cgccggtgat cgtcagcgta aggctctcgg cgtcgtcgta cacgcccgcg gcgatatcga
     6781 ccgtgggctt tgccgccacc atgcggcgcc ccgtgccgtt gtccgcgccg cggctgctct
     6841 gtgcaaaata gaaatacccg tcctccccgt ttagacggcc gtagctgccg ccggccggga
     6901 tatcgcgcag gaacgcccag tcgcagagcg cgccggaagc atcgaagaga tagagctcgt
     6961 cgcgctgcgc gctcagcccc acgttttcgc actcgaacac atagctttca ccgggcgcaa
     7021 gcgtcacggc gggcagcgtg tcgagcgccg gttccttgaa tttatccgag atcgtgtatc
     7081 cggaaagatc gatcgtctcc cccgacacgt ttttgagctc cacccagtcg ggcgaacccg
     7141 cctcgtacac gacgatctcc cacagcagaa gcggcgaggt gcgcgtgtcg gcatcgcaga
     7201 atccggcgta gccttcggcg gtattttcaa gccccggcgt cgggaagtcg gtcacggcga
     7261 agctgccgtc cggctgcatc gcgagcgagg acccctccgg cacggcctcg atcttcacgc
     7321 tgcggatctc cgcgccgttt ccggcggaga gaatgaccgt gtcgccgtcg gtgagcgaga
     7381 agctcgtgtg caactcgccc tcggtgcggt tcttgccgga ggcgaagatc accgcatact
     7441 cgcccggcac aagcgtcagc gacgggatct tccattcgtc cggtacgtcc ggatcgtccg
     7501 agagatacca gcccgcaaga tcgacgctcg tctcgccgag gtttgcgatc tcgatccagt
     7561 cggagaggtc gccgtcgctg tcggcgagcg taccgcggtt ttgcgccatg acctcggtga
     7621 tgtaaatttc gtcgttttcg tagccgccgg aacgcagcca cgcctcatac cccgcgtcgt
     7681 cgttggaata tcccggcgtc gggcggtgag acaccgtcca ttcgccgttt tccttggcat
     7741 aggccgtgtt cttctccagc atcggcagtt ccacgctctc gctctccgcg ccggagggcg
     7801 ctgtgagcac gaccgtttcc ccgcccgcgc tcgccagcgc aaaggacgca tattccgcgc
     7861 cctccacgcc gggggcgcac catacgatct tatattcgcc cgcgccgagc atttcgtccg
     7921 ggaaggcgta ctttgttttg ttcgtgcggt ccgagagctt gtagccggag agagataccg
     7981 gcgaatccga ggggttatgt acctcgatcc agtcgtagaa attcccgtcg ggcgcggcat
     8041 agagcgtgtt tgaggactgg atctcgctga gctcaagcgt gccgccgctg cgctgcgcga
     8101 gcgcctcctg ccacgccttt gcgcccgcct cgctgttcgg gtagccgggc gtcggcgtgt
     8161 cggcgggaat gagcgcgccg tcgctgccgc ggacaagcga ctgcccgctc cggcacgcag
     8221 cggtcacggc gctgtcgagc accgtgcggt tttcgttcat aaggcacacc gtctccccgc
     8281 ccgccttgcg cagggcaaag ggcgcatagt cgccctcctc gtccggcgag caccacacga
     8341 cgagataccc ccgcgccggg acgaccgtcc ccgccgggaa gcagtatttg cctttgcccg
     8401 tctcgtcaga gagatagtat ccgccgatgg agaagtcctt cgaggacgtg ttttcgatct
     8461 cgatccagtc gcagaggaca cccttgccgt tcgggcaggt cttattatcg gtcatgacct
     8521 cgctgatgtg gacgctgtag ccgccgtccc acgccgggcg ctggttatgg cgcacaatgg
     8581 ccgtgaccgt gaatgccgcc gcggcgagta ttgccagtac gatcagccag aggacgatct
     8641 tctttttttc ttttgcattt gtcatggtat tccctccgct cattccttca gccgggcgtc
     8701 cgccgccgcg ccgcgcagcc ccgtgaggca catgcggctg gcgatatcgg aaatctcctc
     8761 gatggactgc cggcgctcgg aattgaacca gctctgatac accgccacca tgccggaaac
     8821 ggcgtagtcc atcatcaccg agatacgctc cggcggcaga tctatgcggc tttcaaagcg
     8881 ttcctgcgcc ttctgctgga gcgtcagccg gatcttatgt gttaaccccg cgttccggcc
     8941 ggatcggagc agcgcgccgt aaaaatccat atcgctgttg atgatctccg tgagcttcat
     9001 gaacaccgcg tacgggttct cgacaaagtc gatctcctgc atcgccgtat caaacgtgcg
     9061 gatgacctcg ttttccagct cgtctacaag ctcgtataca ccgcggtagt tgttgtaaaa
     9121 cgtcttacgg ttgattatcg cgcgcgaggc gatatccgtt acggtgatat cgtccatatc
     9181 cttttcggtc aaaagctccg cgaacgccgt gtggatcgcc cgcttcgttt ttatgacgcg
     9241 aagatcgctc ttccggtttg ccggttccat cgtacgccct ccttttcggg aacaggatcg
     9301 gattccacat cttttttacc aaatgtgtag taatcctcat cgggtgtttt ctctgggact
     9361 tccgccacgg tttcgcgccg gcgtgtccat tgccgcgccg cggaggtctc cttattataa
     9421 tacacagacg ttacataaag caagcagagg agagaaaacc gtgaaagttc tgattttgag
     9481 ctgcgacacg ggcgagggcc acaacagtgc cgcagctgcc gtcgccgggg cgctgacgca
     9541 aaggggcatc gaaagccatg tctttgaccc actcgtgctc gcgggaaagt acgccgagcg
     9601 cttcgtatcc ggcgcatata cgacaattat gaaaaaagcg ccgtcggcgt tcaacgcgct
     9661 ctaccgcgcc ggggatattt acagctcgac cggcatcacc tcgccggtct accacgcaaa
     9721 cgcccggtac gccgcaaatc tgcgcgcatt cattgaaacg aacgggtatg accgcgtggt
     9781 atgcacgcac ctctacccga tggagacgct gaccgccatc cggaaatcgg gagatttcac
     9841 cgtgccgtgc tacggcgtgc tgacggacta cacctgcatc cccttcctcg ctgagaccga
     9901 tctggacggc tacttcatcg cgcacgagga tctgcgcgcc gagcttgtgg aaaagggcat
     9961 tccgtcggat cgcatcatcg tcaccggcat cccggtggac gaaaagttcg cgcatcacat
    10021 ggaaaaggcc gcggcgcgca actatcttgc cataccgcaa aatcaggatg tgttcctcat
    10081 tatgagcggc ggcatcggcg tcgggaacgc gggcgcgatc tgcgacgaga tcctccggcg
    10141 gcacagcgga gcgttcacgg tctatatcct cgtcgggcgc aacagcgatc tcaagcagac
    10201 gctcgaggag aaatacgccg gaaacgagca cgtccgcatc gtcacgttca cgaaaaaggt
    10261 gaatgtatac atgaacgccg ccgatgtgat gatctccaag cccggcggac ttacaagcac
    10321 cgaggcggcg gtggcgcagg tcccgctcgt gcagcttctc gtatataccg cgtgcgaagc
    10381 gccgaacatc gcgttcttct cctcccacgg tctttcggag cgcgcagaaa acgccgccga
    10441 cgccgcagag gccatcggta tcggtcttca ggccttctgc attccgggca gcgttgccga
    10501 ccagcgcaag gtgggcttgn nnnnnnnnna aggcgagagc cgaagccatg cgcgaggcgc
    10561 agcgaaacac cattgcgccc gacgccgccg ataaaattgc ggaaaggatc gtgctgtcat
    10621 gaccggaaac gaagctcttc tcacctttgc tctgatcgcc gggggctttc tgctcggcgg
    10681 cgtgatgttc agccgcgcac tgccgaagct tttgacgggc aaggatgtgt gcgcgctgcg
    10741 cgccgacaaa aatccgggcg ctgcgaacgt gttcgccgtg tgcggcgtgc cgatgggtct
    10801 tttgtgtctc gcgctcgata tgctcaaggc gtatctcccc gtgtnnnnnn nnnnacacgc
    10861 agtcggtctg gtttgcgctc gcgctcgccg cgccggtgct cggccatgcc gtcggcgtat
    10921 ttaacggctt tcacggcggc aaatgcatcg cttcggcctt cggcgcgatg ctcgcgctcg
    10981 cgctcacggg cgtttctctc agcgggcttc tccttctcgc ggggctttac atcttcttct
    11041 ccgtcgtcgt gaagatccgt ccgcaccggc tgcgcagcat actcgtgttc gcgctctacg
    11101 gcgtgagcgc cgcggtattc tattcgctcc acggcgaggg cgcgctcgca ctcggctgcg
    11161 gtatgatcgc cgtgaccgga gtgctgcgtc acacgcggcg cttctgcgtc gccgag
//
LOCUS       DAWAAE010000059        11132 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2623_length_11132_cov_3.054076, whole genome shotgun sequence.
ACCESSION   DAWAAE010000059 DAWAAE010000000
VERSION     DAWAAE010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_11132_cov_3.054076"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2134 a         3294 c         3256 g         2448 t
ORIGIN      
        1 aaaggctgcg gctcgccgca gcgcactcgc ttacgctcgc acgtttgcga gccgataggt
       61 attttctccg acgcacatgt cgccggagaa aatggttgga atctttttgc gcctgcgggc
      121 gcaaactctg cgaggctttt tcgacagtct gaggggagaa ccgctgcgcg gttctccctt
      181 ttggtattcg attggagaat tactcggcgt cggcgagagc cttggcttcc tcgatgacct
      241 tctgctggac gttgccgggg gcctcttcgt agcgcacgaa cttgcaggtg aaggagccgc
      301 ggccctgcgt catggagcgc aggtcgatcg tgtaggagct catctcggcc atggggactt
      361 cggcctcaac gatctgcatg ccgtcctcgg cggtcatgcc gagcaccgtg ccgcggcgct
      421 tggaggagat atcggacatg atatcgccga ggttcgcgtc gggaatggtg accttcagca
      481 ggccgatggg ctcgagaatg gtcggcttcg cgcgggggat gccgtccttg taggcgagct
      541 gcgccgccgt cttgaacgcc atttcgttgg agtcaacggg gtggtaggag ccgtcgtaca
      601 gcgtggcctt gaggttgacg agcgggtagc cggcgaggac acccttctgc acggcgtcgc
      661 gcaggccctt ttcgaccgcg gggaagaagt tcttgggtac gctgccgccg aacacttcct
      721 cggcgaagat catttcctca gactcctcct ggggctcgaa gcggatccag acatcgccga
      781 actgaccgct gccgccggtc tgcttcttgt ggcggccgtg ggcttcgacc ttcgccttga
      841 tcttctcgcg gtaaggcacg cgggcgggct tgagctcgct ctcgacaccg aaacggctct
      901 tcagcttggc gcagaggacg tcgagctgga tatcgccggc gccggagaga accatctggt
      961 gggtctcggc gttgttgacg accgtgaagg agatgtcctc ctcgttcaga cgggcgagac
     1021 cgttggcgat cttgtcgtcc tggcccttcg tcttggggga gatgcacatg gagtagcagg
     1081 gctccgcgat ctcgatggcg ggaagctcga tctcggcctt cgggtcgcac acggtgtcgc
     1141 ctgtcttcca ttccatcttg ccgatggcga cgatgtcgcc gcagcaggcg tccttgacct
     1201 cggtggtctt cttgccgcac atggtgtaca gacggccgag cttatcggtg ttgccggtgc
     1261 gcgaattgcg cagagacata tcggcggtca gcttgccgct gacgagcttg atgaagttgt
     1321 acttgccgaa ctggtcggac acggtcttga acacgagacc ggcggtcggg ccgttgggat
     1381 cgatgccctc gacctcgtcg ggcgcgggga catagtccag aatggcctgc agcagagcct
     1441 gcgtgccgac gccggtcgta ccgacgccgc agaacacggg aacgacgctg cgatccttca
     1501 gaccggcctt gatgccggtg acaagctctt cctcggagag ggtcatttcc tcgaaatact
     1561 tctccatgag ctcctcggta gcgcccgcgg cggtctccat aagagcctcg cgcagctcct
     1621 cggccttgcc ggcgtcggcg gcggggacgg cgaccttcgc ggtcttgttg ccgttggtct
     1681 catacgcgat gttgtgcacg agatcgatga cgccggtacc gtcggaggcg gggatcgtca
     1741 cggggcagac gatgctgccg tacttggcct gcagcgcttc gagcacgccg aagtaatcac
     1801 cgtgctcctc atcgagcttg gagacgtaga aggcgaccgg cttgccggcg gccttgagca
     1861 gcttccagtt gcgctccgcg ccgacggaaa ggccgtcctt cgcggtgcag aagatgatgc
     1921 ccgcctcgac agcgcgcagc gcggcggcga cttcaccggc gaagtcctgg aagcccgggc
     1981 agtcgatcac gttgatcttc ttgccgccga aggaaatggg agcgatagcc gcggagatcg
     2041 ttacctgacg cttgatctct tccgcgtcat agtcacagac ggtgttgccg tcgttgacct
     2101 tgccctgacg gtcgatggca ccggtcaggt agagcatgtt ctcggtaagg gtggtcttgc
     2161 cgctgccgcc gtgaccgagc aggcagacat tgcggatgtc cttggttgtg tagctcatgg
     2221 gttgtcctct ccttataatt atcttaaaaa actttaattc attgtcctgt gaatcggcat
     2281 tgtcattctt ccgaaaaaac aggggagcgc cacgaggata aattatacac tatgacagtt
     2341 tttttggcaa taattatttt tgcagcagcg tccgcagcgc cagcggcacg atgatccacg
     2401 cgagcatata gagcagcaca cgccccgcgc cgatcgccgt ccacagtccc tgccacgccg
     2461 ggaccaccgc aaggatcgcg ccgagcgcgg cgcagatgac gcagcaccac gcgcacgcgc
     2521 ggccgtagga atggagcttc gtgccgcatt cggcaaagtc cacgagtgtg tcgagaccgt
     2581 cctgcgccat cacaccggcg gcagggctgc ccgccgaggc ggggatgccg gaaatgcggt
     2641 accgctcggc ggaagacggg aactggaagc cctcggcgga tacgccgaac gttttctgca
     2701 gaagaagagg gtcaagatta aagtcgcgca cggcgaagat cggcttgcga tgctcgcggc
     2761 gcagggcggc caaggcgcgc tgcacgctcg ccatcgggcg gtagcgcagc gggaacaccg
     2821 ccgccagctc gccggatacg gcggtataga gcgcgttttc ctccttgatc ttgtcgggga
     2881 tcttcacgcc gctcagatgc atatacgcga gcgtgccgat gcggacctcc tcgccctgca
     2941 ccataccctt cgcgccgccc tcgccgacgg agaaatcctc cacggtctgc atggcggcgt
     3001 ttttccggcg catcagctcg gcaaagggcg cggcaagacc cgtccctgcc gcatcgagca
     3061 tgctgccggt gatgccgagc acctgcgcgg cgcggctctt gtccataaag cgcacgcccg
     3121 tgatctcgat ggcgtcctcg gggaaaatgt cggtatcggt gagcacgagc cggcgcgacc
     3181 ggccgacatc gcgcgcgccg gaccatccgg ccagagcgct gccgcgcatc atgagatggc
     3241 gcgccgtgcg ggagaatagg atcgggaacg agagcgtcca gttgaacgaa acgcaaagcg
     3301 ccgtcataag cgagaagaga tggaaaaacg ccttcatatc ctgcgcgccg agcgagagcg
     3361 ccagcgccag cgcgagcgag ccgacagcca tcggcacaaa cgccgccatg gcgagcgctt
     3421 ccgcgccgct cggctcctcg ctgcggcgga caagaccgtc cgacgagcgg cgtgacttga
     3481 tgaggatgcg ctccttcttc cccggcagca cctcggaggt gacggcatag gcgtccttgg
     3541 cgtgatgcag ggcaaggaag gaatcggcgt acgcccggca gctgtaccac gcgccgcgca
     3601 gcgcgaacat gaccgccgcc gcggggatca ccgcgaaggt gacgccgtgc gagccgtcgc
     3661 cgctcgcggc gatatagatc acgtccgccg ccgcggcgat cgcggcgaga acgatcatgg
     3721 attccgcccc ggcgcggccg cggatgagcc ccatgacgcc cgccgtcaca acgtccagcc
     3781 cgatgagcat caccgagagc atcccgatga gacacacaag cgacgtcgct ctcgcgttcg
     3841 tgccgagact gccgggcagc ggcaggccga agtcatacga aagagtgatc cacgcgagca
     3901 gcacgcagac tgccgccgcc gcaaagaagc gcaggcgcaa actcggcatc tgcgcggcat
     3961 aatgcttcgc cgcgcgctgc ggcggcattt caagcgttac gtcctccggc tccgggtcgg
     4021 gctgggagag atcgttcgcc cggcggatcg acgccgcggc gagaagcccc acgagcttgc
     4081 cccaaggccc cggcgcgcgg ccccgcccgc ggcgggatac gctgccaagg ccctttttct
     4141 ttttgctttt tttctccggc tcgtccggct cttcgccttc ttccgaggct tccggctcgt
     4201 ccggcgcttc aaggggcggg gcataaaagt ccgcgtcgtc ctcaaagccc tcgtcgtata
     4261 tttcgccctc ggcggcctct tccggctccg gcgcgtcctc atagtaccgc ccgtccggtt
     4321 ccccggtctc ctccggctcg tccggcgcgg tgtccggctc ggtctcatac tccggcgcgc
     4381 cggtatcaaa ttcttcgtat tcggggtatt ccgcttcttc gtccggatat tcggcctcat
     4441 actccgtctc tgccgggtaa tccgcctgtt cggaggaata gccctccgtc tccggccggt
     4501 attccgtttc cgccgtttcc ggcgcggcgg tctcctccgg cgccttgtcc cggcactcct
     4561 cgagaatgct ctcgagcgag aagcccggca cgccgaagct ccggggaacg ccgcctgtat
     4621 cgcgttttgc catagctccc tccgtctcag gaatctctgc gctggcggac gacctcatac
     4681 ataaggatcg ccgccgccgc ggaggcgttg agcgatgtga tcttgccgag cagggggatg
     4741 cccacggtga agtcgcactg ctccgaaacg aggcggccca tgccgaagcc ctccgacccg
     4801 atgacgagcg ccgcggggcc ggtcattttc gtctgccaca gatcggcggg agcgtccgcc
     4861 gccgtgccgt agacccagag gccctttttc ttcagctcac cgagcgccgc ggggatgttc
     4921 gtcacgcgcg agacgagcat atgctccgcc gcgcccgcgc tcgccttgtc caccacggcg
     4981 gtgagcccgg cggcgcgccg cttggggata atgacgccgt ggacgcccgc gcactcggcg
     5041 gtgcgcagaa tcgcgccgag attgtgcgga tcggcgatct cgtcgcacac gacgatgaac
     5101 ggatcctcgc cccgctcggc ggcacgctcc agaatatcct cgatggaggc gtactcgcgc
     5161 attgccgcct gcgcgatgat gccctggtgc gcgcccgtgg cgctgagaga atcgagcttg
     5221 cgccggtcgc actcggtgac gacgatgccg ttttcccgcg ccttggcgag aatgcgcgca
     5281 agggtgcggt cggtgtcgcc gcgggcaacg aaaattttat cgatcgtccg cccggcgcgg
     5341 atcgcctcgg caacggcgtt gcgcccctcg atcaggtctt ccctgtcctg acggatctcc
     5401 tgttcacggt tttccatatc gttaaattcc ttctttatat tttcatatta acaatgcgca
     5461 taggtatacg cataggtata gataatatat tttaccacac gatcccgcca tctgcaacgg
     5521 aaagcgccac ttttgctttt tcttttttct gccgtcttga cctttccgtc cggaatcggt
     5581 atgctgagag caagaacaag cgaagggcgg tgagcgcatg ctctgggaca ccatccccgt
     5641 gccgaagggc acggacgccg attttgccgt tgcggtcacg gacgattcgc tcgcgccgtg
     5701 gatcaaaaag ggcggcacgg cgtatctccg gcggcgcacc gagctttacg acggcgacat
     5761 cgggcttttt gaaacggacg gagggatcgt cttccggcag ttctgcgccg acagccgcgg
     5821 cacagtgtat ctcttcgccg tgaaccgcgc ccacgccgaa gacgaccgca tgatcccgcc
     5881 ggacaaagcg gcagagctcg tctgctacgg ccgtctggag ctaaaaaagc ccatcccgct
     5941 cccgatggat tgagtatatg ataggaaaac cgcagcggct ccaaaattgg aaccgctgcg
     6001 gttactttta atcgtcctgc ttcatgctca tatcccgaaa aagaacccga agataatcac
     6061 gacggccata taaaactcgg tcgataaaaa catctcttcc gttcacacga tagaaaacca
     6121 tatagttccc gctcacaaga aaccgatagt ccccgccgac gtcggcgacg gaggacagca
     6181 gagcgcccat ttccgcaaaa tctttcaagc gttcaaccgc gtccagaatg cgatccacca
     6241 cgcgctccgc cgccacacgg ttttgaagct ccgacactat ataccacacg gttttgaagc
     6301 tccgacacta tatagtccca tatatcatcc agatcacggc gggactccgc tgaataatgg
     6361 atgttattct tcattcgctc tggcgcggaa gtgcgctctc atgtcctccg gggtcagata
     6421 gccttcggtc tcaccggatc ggcggccctt tgcgagctca ctcataagct tcagcgtggc
     6481 ctgcgctttt tcgaaatcct gaatgtccac aatggcgtac cggccgcggc cgttcttcgt
     6541 caggaaaacg ggcgcacccg ccgctacatc ctgcaatact tccgtgtaat tccgcagatc
     6601 ggagatcggc ttgatgttcg gcatagtgat caactccttt gcgaatagta tacgccgttc
     6661 tgctgtaaaa ttcaacgata ttttttacag cattttatac taccgcgatc acacatagcg
     6721 cgtttttgct taaatcactg caatttcagg aacccctgca gaaagacgtt agggatctga
     6781 aaccgctttt cggtatccgg aaatgccacg gtgatatgcc ggaatttgat gtcgtcggga
     6841 tcgatcgcgg tgatcgtgcc gacgccgaag gctttgtgca gcactgccgt gccgacggag
     6901 agagaggata cgtcaaagga cggcttgggc ggcgcggcgg ggacagcggg agccggcgcc
     6961 ggttttgccg cggcagacga aacggccgca gccgccttcg gcgcgcggtt cgccgcggcc
     7021 gccggatgga tgacgaccac ctcgccgttg cgcccgccgc gcttctttgc cggtttttcg
     7081 ggaacaggcg gctcttcccc gtcctcctct tcctcggcga cgaaggcgaa cgggttttcc
     7141 ggcatatcgc cgtcatcggc cggaggcgct tccatggagg actgctggtc ggcaaacatt
     7201 tcgtcaaagt ctaccactgt gctgaaaagg ccgacctttt ccccggcatg gcggttgatg
     7261 ccggtatagc tgagactgga atcctcatag cgcttgaatt tgtacaccgc gtcgttcata
     7321 agcgattcat tgaagacgcc gagcgacatg agaagctcaa aatctctggt atccagaccg
     7381 gtgacctttt tgaaataatc cggctccaga ttggtgataa cgtcgatcat ggtctgctca
     7441 cggtagtcgg tgagatacat gaagatcggg atacgggtag cgaacttgat gagcttttcc
     7501 tggatctgtt tgcggagggt cttatattcc ttttcctcgt cggtgatctt tttcttctcc
     7561 gccgggttct cggcggtgcc ttcttttctg gccttcttga ccttttcaga cttattgatg
     7621 atggtctcaa tgtcctgatt caggctgcgg aagccctcga tcttcatgag ggctgccata
     7681 gcctcctgat tggccataag ccgggaaagc gtgacattgt ccacattgac gagaagcgcg
     7741 ctctcccagc ggcgggcgag cagcgtggcg gaggtgccgg acatggcgta atcgaggatc
     7801 tccgaggcgc tgatctggcg catggagctg ccgtcgtagg caatgaccgg aaggaagttg
     7861 ataaattcct ccaccttttt ttcggggctg acttttccgt tgggattcag ccggcagctg
     7921 tagtcggaga tctgcttgag cgcccggtcg agagcgaagt caaatacata gcactcccgc
     7981 ttcatgatct cgccgtcgat ctcccacggg ctctgcacgc ggaacgccgc ctgaaaatag
     8041 gtttccgggc ttttgagatt gcgcagcatg aagatccccg tccacggctt cacggtaacg
     8101 ccggtcgtca gcttgccgca ggtgagcgtg atggtcttgc tctccagcgg gtcggccatg
     8161 gacttctgca ccggggcaag agcggaaagc ccgatgcctg cggaagctcc ggcgcacata
     8221 ttgacggtgt aatcgtgata aaaggcgttc tgccgctgcg cgagcagatt tcgcattgcc
     8281 tcgcaggaag aaatgctcgg caaaaaccag acggtgtgcg aaagaacgtt gagaagccgc
     8341 gcatcggaaa acggcatggc ggggcgccgc gcgccgagct tcagctcgtc caccgccgtt
     8401 tcgagatacg cgccgcggat gagatcgagc catttctgca cataatcctc gtagacgaac
     8461 cgggcgtttt tgcctttccc ctccgctttg aagaatacgt tcagatcaaa ctcgttgaac
     8521 tcgccctcct cggcgatctg gcggatgctg tcgggaattt tgtaggtcat catgaccatg
     8581 cgggggagcg aagcataggg attcttttcg ccgacccagt tttccttggc gcgctgttcg
     8641 tcggagtagg tccagttata gatctgctcc tcgatgaatt cgccggaatt gagcgcgcgg
     8701 aacggcgtgc cggaaagata gagatagtac cgcgtggtga tggggagcca cgtttcgtcg
     8761 caggcgttgt tgttatcaaa gccctcgccc tcatccacgg agtcctcgtc ctcgccgtca
     8821 aagagctttt tggcgctgtc cttccatgcg ccgaaatgat actcgtcaaa aatgacaagg
     8881 tcccagttga tggtatgtac ccactcgttg tgcgctttga tgccgccgag cttgttcagg
     8941 ccgagaaaat cctgaaagga gccgaagcag acgatcgggc ggctcttatc cgcgcgttcg
     9001 tactgctggt cgatggtcgg ctcggcggga ttctgcggcc gggcgatgaa ctgccagccc
     9061 tcaaaatcca catgcgtgag aaggtcttcc ttccacgcgc tctgcaccgc gggcttgaac
     9121 gtgaggatca gcacacgggt aaagcccatt ttctttgcca gctcatacga ggcgaacgtc
     9181 ttgccaaagc gcattttcgc gttccagaga aaccggggaa tctgcgccgt cgcctccccg
     9241 tcggtggagc ggaaatatgc catcgtcttc gcgacggcgt ccgcctgctc cgggcgcatg
     9301 ggaaaatccc gtgtgcggtt ctcgctgttc tccgtccggt ttttcaccgc catccacgcg
     9361 gcgcggacat cgtccgcggt gcagcggaac cattcgccgt ccacgtgccg gatgccgtgg
     9421 cgcacaagat accggtgaat gtcgtggtcg gtaaagcagc cgccgtcaca gtacatggcg
     9481 gactccttga acacgatctt atagggcacg ccgccggggc ggagcgtcgg gtactgctgc
     9541 gccacgcgcc gctccacatc gctctttgtg tagccgacct tcagcaggcc cttatagttg
     9601 gggttgttct cctcgtaggc gtagatcatc ggatgagcat ccggccgctg ggggaaaaac
     9661 tccgcattac tcatcgccgt caccgcccaa ctccatcggc tttatcatgg actcgataaa
     9721 agcgatctcc tcgtcggtaa ggccgtattt ggcgtagagt tcttcatctg tccatggttt
     9781 ggagaaatct tgaaggggga caaactgata gacgcctcga ggcccattct gcgtcttttt
     9841 cttaatactt acaagatatc ggaaaaaacg tgtttttata tattctaata tattaaggca
     9901 ttcttccttg ctgaaacgat gagattcatc atagccaatc actaaaaagg tttgtgaaca
     9961 tacgcttcca ggctcaccat aaaacggttt gcctaaaacc tgttcgtcga atccgctgcc
    10021 accagcagca gggataaata ctttatggaa cttttgcact tctttgtttt ttggaatctg
    10081 agattcagat atccatctgt aagttctctc cctgtctatg ttcaggtaat acttgacaca
    10141 gtagacatta tcttttattg gtttatagcc tgtccagttg gaagtaagaa ggatgctgtt
    10201 atcgaagaag tgctttgcgc ttaccagtcc ggaaaaattg gtttccgagt ttttataata
    10261 tgctccttcg atgccttcaa tcttttgtat aagtgaatat gcacgcgggt ctctaataac
    10321 aatcccagag ttcatcgagt ctaggtaatc taatcttctt tttatacttt ggcttgacgc
    10381 ttcatgaaag aaataatcac acataccaat gtaatctcta tcccaaagaa agtaactgac
    10441 gccacctttt attgaaacac cgggaaaaca atctgaggcg ttctcaaaat cgtggataac
    10501 tcgcattttg ttgcttttta tcatcaagtc cacccacgcg tcagggatgc cctgcgccgt
    10561 cctagtcatc cacctgcttg gtgtaatcat gcacaaatac cttggattga gcttaatcgc
    10621 ctgcgaaata aatagattgt aaatcgattt ggcatttgca ctattgccgc cttcaatgcc
    10681 gaaactcatc tgatatggag gatttcctat aatcacatca aacttcatct tgaaaatttc
    10741 ctcaggtctg gtcgtgtgga tgaattcgta tgcgtggctt tcgagcgaag gatcgcggtc
    10801 atattccccc tgcgacgctc cgcagaatac gcatttcttg ttctgccagc ggtgctgtat
    10861 gcgtttgtac cgtatattcc cgctcgggtc attgaaccgg gaaacggagt atttgctgtt
    10921 ggggtatttg gagcagtaga cgctccggcg ggacaaaagg ctcgtaagct ccgtaatggc
    10981 gatgccgaag agctgtttat ggaaaatatg atccagcgtg ctttgcagct tttcctgaaa
    11041 ttcttcctct tcggatgtca gcggttcgcc gaagctcttt tttgtatcta tggtcttgac
    11101 ccgctgcttc cagccgggca gcagtccgtc ga
//
LOCUS       DAWAAE010000060        11055 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2648_length_11055_cov_3.097455, whole genome shotgun sequence.
ACCESSION   DAWAAE010000060 DAWAAE010000000
VERSION     DAWAAE010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_11055_cov_3.097455"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3401..3410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4584..4593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2404 a         3452 c         3211 g         1968 t
ORIGIN      
        1 cagcagccgc tcggcagcac ggacagtgcg tttgagatcc gcttcgagca cgtcatgtat
       61 caggataagg acggtcaggg cggctcaaag tacccggtcg atccgccgtc ggtgacgatc
      121 gctcttacga tcgcgccgtc gatccccggc gacgtggacg ccgacggcaa tatcaccgcc
      181 gaggatgccg cgaaagtggc cgagcacgtg cgcaataccg aaagcaagaa tgccggcggc
      241 ttcacgctga cggagacgga gatcaaatac gcggacgtga acggcgacgg gaaagtgacg
      301 atggaagacg ccgaactcat ctggcagtac agccgtggcg agatcaccga attcccgaaa
      361 aaggaaacga ccgaaacaac gaaaaccgaa taaaaccaca aagcccggcc atccgaagat
      421 ggtcgggctt ttcaaatgcc tcccttgtgt aaagggaggt ggcaaaaccg caggttttga
      481 cggagaaatt gcagagcaca gagtgttccg aaagagaaca atccctcagt tagcctgacg
      541 gctgacagct ccctttgcac aagggggcct ctttttacag attaagcaaa tcttaaagaa
      601 atcccatctt tattattaag cgtgggaatg ctatgctgta gctgtaaacg agagagggga
      661 agagccatgt acaaaatact catctgcgac gacgagcgcg atatccggaa cgcgctgcgc
      721 atctatctta cgggcgaggg ctacgccgtc tgtgaggccg acagcggctt agcggcgctc
      781 gagctcatgc agcgcgagga agtacagctc gtgctcatgg acatcatgat gcccggcatg
      841 gacggcatga cggcgacggc aaagctgcgc gagcagagca acgtcccggt catcctcatc
      901 accgccaaga gcgaggacaa cgacaagatc ctcggtctca ccgtcggcgc ggacgattat
      961 atcaccaagc ccttcaaccc tcttgaggtc cttgcccgcg tcaaggcgca gctccggcgc
     1021 tatgcccagc tcggcggcat gccggagaaa agcaccgcgc tcacgctcgg cggcatcgcc
     1081 atggacgatg agcgcaagag cgtcacgctc gacggcgagg aggtaacgct taccccgacg
     1141 gagtacgaca ttctccgtct cctgctcgaa aaccccggca aggtgttttc gagcgccgat
     1201 atctaccggc gcgtatggaa ggacgaaccg atttccgcgg agggaacggt gccggtgcat
     1261 atccgccatc tgcgtgaaaa gctggaaatt gacccggcga acccccgcta tctgaaggta
     1321 gtctggggca aaggctataa gctggaggaa atgaaaaaat gaaaaagctt acgcatagtg
     1381 cggcggcacg cgccgtcgcc tttatcctgt gtctgctctg cggccttacg gcgctgttct
     1441 gcgccgcagg atatttcatg acccgcatcg accgcgaccg cacgctgcct gagccggaca
     1501 gcgtatcggt gtataagaac ggcatccgcg ttctgctcaa caacgcgctg caggaggaac
     1561 ggtataccgg cgaatacccc gagcttccct ccggctactc ctatgtagca acggatacgt
     1621 ccaccggcaa ggtgctgtac gacggcagaa cgaaaaacgc cgtgccgctc ggcatttccg
     1681 gcggctgttc cagcggagac agatggatcg acgtgcaggc gtatctcgaa ccgaagcaga
     1741 cgcccggcga ggagctgacg ctgctccgca cctccgtcgg cacgctgctg cgctatcaga
     1801 atgcctatct ccccgtgggc atcatcacgg cggtgctgtg tctcgcgctc ctcgtcttcc
     1861 tcatggcgtc catgggcaaa acgccggacg gcgtgaagct gcgcggcatc caccgcgtgc
     1921 cgctcgatct ctttctcgcc gcggcgctgg cggcggtggc cgtcccggca aacttctatt
     1981 cgggaattaa cctgctctat aacccgctct gggtatggat gatgccggtt acgctcatgg
     2041 cgctctggat cggctttgtc gtgctgctca cgctctgcac gatctcggcg cggctccgcg
     2101 ccgggaagtg gtggagaaac agcatcatct atatcgtctg ccgctttgta taccgcgccg
     2161 tgcgcggctt tgtccgcgcg ctgcccatcg cgtggaaggg gatccttgtt tacgccgttg
     2221 ttgtgctggt caatgttctt ggcatgatac tcgctttcgg cgcgggcagc tttttcgtct
     2281 tccttctgac gcttgtgctg gacgccgcgg ggctgtactt tgtcatccgt atcatgcgcc
     2341 agctcaaggc gctgcagacg gcggcgcaga agctcgccgc gggcgatctc acctacacgg
     2401 tggatacgga gaaaatgtac ccggtgctca aggagcacgg cgacgatctc aacgccgtga
     2461 gcgtcggcat gagccgcgcc gtgaacgagc gcatgaagtc cgagcgattc aagaccgagc
     2521 tcatcacgaa tgtcagccac gatctcaaaa cgccgctcac gagcattgtg agctatgtcg
     2581 accttctcaa aaaagaaccg atcgaaagcg aacaggcgca ggaatacatc gaggttttag
     2641 accgccagag ccagaagctc aaaaagctca cgaccgatct cgtggacgca agcaaggcga
     2701 gcagcggcgc gctgccggtg aatctcgaaa agatcgatct cggcgagctg ctgcgccaga
     2761 gcgcggggga gtataccgaa aagttcgccg cggcaaatat cacgccggtg ctgaatgtgc
     2821 cggaggcgga aacgtatgtc acggcggacg ggcggctttt atggcgcgtg ctggataatc
     2881 tgctgggcaa cgcggtaaaa tacgcgcaga gcggcacgcg gctctatctg gagctgacgc
     2941 cgggcgagac ggaaacggtg ctcacgctca aaaatatttc ccgcgagccg ctgaacatcc
     3001 cggcggagga gctgatggag cggttcgtcc gcggcgacgg ctcgcgccat acggacggca
     3061 gcggactggg actttccatt gccaattctc tcatggaact catgggcgga aagctcacgc
     3121 tgacgctcga cggcgatctt ttcaaagccg ctcttgtgtt tccgcagaaa accgtataat
     3181 atttttgccg gacggggaac caaacgaccc cgtccggcgt ctatatttcc gaacacccaa
     3241 aggtgcgagg aaaggaaaga gcttatgaaa atgaaacgat ttgctgcggc gcttctggcg
     3301 cttattctgt gcgcggcgtt tatgacggcc tgctcgccga agccggagca gcccgccgca
     3361 acatcaggca atgcgtcgtc cggcaacgca gccgccgaac nnnnnnnnnn gcctttacga
     3421 cgcgctgtgc gaccttttca acaactatca tgaaggcacc gccggctgct cgctcacggg
     3481 agcgcgcatt gctgccacga tcatggactt ctcgctcacg aacggcatgg acgccgtacg
     3541 cagcggcgcg gtcgcctttg atctcggcga ggagacggag ttcggcgaaa agcttaccga
     3601 taagctcacc gcgatgtatg agaccgccat gggcctttac ggcgagagcg ggaaaaacct
     3661 ccttgccgac ggcggctata cgccggtgca ttacccctac agcgccaagg acgtgcgcga
     3721 tacctatacc gcgatcttcg ctgagcgcgg ctgcgaagcc cccgccgtgg tgcgtatcta
     3781 ccgcagcgac gcgaacgccg agcacttcct cgccttcggc accgcgctcg acggcacgga
     3841 gatcaccgcc gaaacgctca acggcgcgat ggacggcctt gtctttgaaa acggcgcggc
     3901 gttcaacacc gttaccgcag acaaggacgg ccacatcaaa gccgacctca acgacgcttt
     3961 cgccgcgcag gtgcgctctc tcggcacgag cggagagtac ttcctcgtcg gcggcatcgt
     4021 caacacgctg ctcgacgcct ttgacggcac ggacgtcacg ctcaccgtga acggtgcgcc
     4081 gctcgagagc ggacacaata tttacgacta cgcgctcacg cgttatgagg agtaactata
     4141 actgtatgag catgaaaaag aaaggcctgt ccctgctgct ctgcgcggcg ctttcccttt
     4201 cactcgcggc gtgcagcaca aagccggaaa ctccggcaca gcccacggcg gaacccacgg
     4261 cggtctccgt gaccgccgcg ccggagacta ccgccgcgcc cacggagacg ccggcggccg
     4321 aaaaccgcaa tgtgccggtg atttccgtcc ggtgcgagaa cgaggatttt tataccgcgg
     4381 acaatgcggc gcttcttctc gtctatgcct gcgccgagcc gagcgtttcc atgaacggaa
     4441 acgacgcggc cgctgccgcg atcaacgaag cgctgcataa gcagtatacg gattttgccg
     4501 tcggtgccga atcgggcagc gaatacagca tttccggcaa ggaaaattat ctggccgcgg
     4561 caaaggaaga actcgcccgg cagnnnnnnn nnncccggca gacggaaaac ggcgatgcct
     4621 cgggcttttc ttcctacagc cttatgcgcc agacaaacgt ccgctacaat gcccggtacc
     4681 tgctgagcat cacctacgat gatacgtcgt atctcggcgg cgcgcacggc tacaccggcc
     4741 gctgcggtca caccttcgat atccgcaccg gccgggagct gacgctcgcg gatctcacgg
     4801 ataactacga cgctttcctc tccgccgccg tcgatcagct caaggatatc atcagctacg
     4861 gcgcagaata cgccgcctac ggtctgaacg atggctacga ggatcagctt gccggtctct
     4921 tccgcgacgg caactggtac tttaacgacg agggccttgt ccttatggcg aacccctatg
     4981 agcttgcaag ctacgctgcg ggtctcattg agttcacgct gccgtacgcc tggctcgcct
     5041 atcagatcaa ggcggattat ctccccacgg agagcacggc ggacggctcg ctcaccgctg
     5101 agctgagcga gagcgccgat gcagccggga acgcaacgta tgtctacgac accggtacgg
     5161 gcggtctcgg cgcgtgcgtc acgttcaccg cctccggcac ggtcgaagac gttgagctca
     5221 acgccgtgac gtatctggaa tactccaaca cctaccgcac ggacggcacg ctctggtacg
     5281 ccgggcgtct cgccgacggc gagagcgtct gcgtgcagac gtggatcccg gacgtgatgc
     5341 cgaatctcgc catctcctgg cgcgacgccg atggcgagca cgtaaagtat atctcccaga
     5401 gcgggatgga cggctctctc atcctcatgg acggagagca gtatgccgag cgtccgctga
     5461 acatctccgg caagagcgtc tatcattacg atatcaacgg cgactatgcc gccgaggaga
     5521 tcaccgtgac ggcgaaggat gccggcggca tcaacgagta cacgatcgcc gtgaacggca
     5581 cggtgctcga ccgcaccaat atgcccgtgg gcgaccggta cgacctctgg atctgcgatc
     5641 tggacggcga cggcatctgc gagcttctgt ttgccgccga ccccggcagc gacgattacc
     5701 gcacctgcgg ctggcacggc gacacgctcg agcccatcca atttaccggc gacgcgcgct
     5761 acggcgcgga cccgaacggc attaccggca gtctcgacgg acgcgtcgtg ttcagcggcg
     5821 gcatcccgat gctcgaggcg tggtactatc agctcggcac ctactgcgcg gtcatcggta
     5881 tgaacggaac gtccgacggc gtcgtaacgc tcgatccctc ttttaaatgg aactaccgcg
     5941 gcagctgcta ctatctgacc gtggcaaaga tcctgccggt gtatctcgat gagggcggca
     6001 ccgccgtgct gacgccgggc gaaaagctcc tgcttaccgg cacggacgga cagaacacct
     6061 tcttccgcac cgatgacggc cgcaccggat cgatccggcc ggaatacgat ggggaaggcg
     6121 gctggaccat caacggcgag agcgaagaga atttctttga gatgcttccc tatgtcggct
     6181 gacgccttca aacgaacccc gtctgtcaga caaaatgacc ggaacacaga aaagaacctg
     6241 tgttccggcc ttttttataa aaaccggttg cagagccggg gaaaaacggt tataatgcag
     6301 cgcagggcct tggaatcgtt tccacggcac tattccatca ccgaaaggac agagaaccat
     6361 gatttattct ccgcaggatc ttctgggcac cacggaatct ctgctgaaat atgacgagga
     6421 cgagcgcctg agcgaggcct cgccccagca cctccacaac gctctcgggc gggcggtaat
     6481 gatcgcggtg ggggacaact gggccaaatc ccgcgcccgc cgcgccggaa agcgcaaggc
     6541 gtattatctc tccgccgagt atcttgtcgg gcggctcgta tacaataacc ttttcaatct
     6601 cggcattctc gacgaaatgc gccgggtgtt tgccgagcgc ggcatcgatc tcgcctgtct
     6661 tgaggagatc gaggacgcgg ctctcggcaa cggcggcctg ggccgtcttg ccgcgtgcta
     6721 tatggacagc gccgccacct gcggcgtgcc gctgagcggc tacggcctgc gctaccgctt
     6781 cggtcttttc aagcagagct ttgaaaacgg cgcgcagaag gaatccgccg acgactggga
     6841 aaagcacggc gatccctgga gcttccgccg cgagaagcac accgtccgcg tccgcttcgc
     6901 cgaccagacg gtgctcgctg tgccgtacga tacgccggtc atcggctatc ataccgacaa
     6961 cgtcgcaacg ctccgtctct ggcagtgcga ggccgagcag gagctcgact ttgacgcgtt
     7021 caactcgcag gattacgagc gcgcgctcac cgagaaaaac cgcgccgagg acatcacccg
     7081 cgtcctctac ccgaacgaca gcaccgtcga gggcaagcgt ctgcgcatca agcagcagta
     7141 cttcctctcg agcgcgtcgc tgcaggacat cctgcgcacc tacaaatcct ccggcaacgg
     7201 cgatcttcag cgcctcggcg agttcatcgc cgtgcagctc aacgataccc atcccgccat
     7261 gtccatcccc gagctcatcc gtctgctcat ggccgagggc atcgactttg accgcgcctt
     7321 tgctatcacc cgcagcgttt tctcctacac gaaccacacc gtcatgggcg aagcgctcga
     7381 gcgctgggat atggatcttc tgcgcagtgt tgtgccggag atctgcgaca ttctcctgaa
     7441 gatcgaagag cgcctgcagc aggagttccc cggcgcgaat ctccatgtcg tctgcgataa
     7501 tcaggcgcgc atggctgatc tttcggtgta tatgtcccgc aaggtgaacg gcgttgcgga
     7561 gatccacagc cggatcctcc gggacgatct cttccgcgcg tggaacgaga aataccccgg
     7621 ccgcatcgtg aacgttacga acggcatcac gccgcgccgc tggctcggtc tctgcaaccc
     7681 ggagctcacc gagctcatcc gcgccgagat cggcgagggc ttcctgcgcg atctcgaccg
     7741 catcggctat ctgcgcgagc acgtgagcga cgagctcgcc atgctcttca acgccgtcaa
     7801 gctgcagaag aagagccagc tcgccgcgca catccgcgcc gcggagggcg tggacattcc
     7861 cgaggagttc gtgttcgacg tgcaggtcaa gcgcgcgcac gaatataagc gccagctgat
     7921 gaacgctctc tgccttgccg acttctattt tgagatcaaa aacggcggtc ttgccgatat
     7981 gccgcccacg gcgttcattt tcggcgcgaa ggccgcgccc ggctaccgcc gcgcgaaggc
     8041 cgtcatccgc tatatcaacc gtcttgccga gcttgtgaac aacgaccccg acgtgaacgg
     8101 caaaatgcgc atcgtgttcg tgcagaacta caactgctcc tacgccgaaa agatcatccc
     8161 cgcggcggac atctccgagc agatctcccc ggcaggcacg gaggcctccg gcacgggcaa
     8221 catgaagctc atgctcaacg gcgctgtgac gctcggcacg atggacggcg cgaacgtcga
     8281 gatcgtccgg caggccgggc tggagaacaa ctacatcttc ggcggcaccg tcgaggagaa
     8341 caacgccgtg cgcgatacct acgaccccga ggcgctctac gattccgacg agcgcatccg
     8401 ccgcgcgatg gatacgcttg tggacggcac cgtgccgacc gacgacgatc agaaggagct
     8461 tttcgacgcc atactcaagg gcgcgagctg gcaccgtccg gacaactatt tcgttctgcg
     8521 cgattttgaa agctaccgtg aagcccgcct tcacgcgctc acggattgga aggatcgcgt
     8581 atccttcggc aaaaagtgcc tttacaacat cgccgcggcg ggcatgttct cgtccgaccg
     8641 cgcgatccgc cagtacgcgg aaaccatctg ggagctgcac tgaaccggca tacaagatca
     8701 aaaatgtcct ctgcgcaaat tggtgcgcag aggacatttt cattttcata tgctcgtttg
     8761 aacggtcatt ccgtttcgtc cggcagaaag ggggcgaacg gcttttgcat gagccagaga
     8821 tcggtgtagc acgtcacggc cgagagcagc acgagccaga gaacgctcac gccgtaaaac
     8881 gccgccgggg atacggcaaa gaggatcacc ggcagcagat ggatcaccgc catgagaagc
     8941 gtccgcggca gatacccgac gccgagaagc accgcgtttt ttattgtgtt ccagagcgta
     9001 ttttcaaagg tcgccgtgag cgggaacgcc cagatgaaca ccatcagcca cagcgcaaag
     9061 cagacgcccg gcagcacgag ccagaacagc tgctccggga aaagcgcgcg gagaatgtat
     9121 acgtccgcgc cgaggagagc gagcgccgca agcagaatga gccagagcag cgtggatttt
     9181 ttgaaattat cgcggaacgc gcggaaaaag gccgcgctgt tccaatgccc cgattcgcgg
     9241 atcatgttcc gcaccgccgt gcagagcgcc gcggtcgaag cgccgagcgt gaaaaccggc
     9301 agcgagcaca cgacccacag aatgttcagc cacgcgagct ccgccgcctg cgtgagaaag
     9361 cgcaggatgg gggaatccgg atcaaagatt tttttcatat tgccacctcc gggagaataa
     9421 gcctattata caggaacaaa tgaccgcatg acaagaccaa ccgcacaaaa agttgacgag
     9481 gtttttgtgt aaatgacata aagttaagcg ttttcgccga aacacacttg caatatttct
     9541 acaaagagga tagaatacag acaagcttgg ttggaatcgt ttccaattca gagctttact
     9601 tacggaaacc cattaaaaat taggaggaaa caaacaatga agaagcttct cgctgttctt
     9661 ctggctctgg tcatggttct gagcctcgtc gcctgcggcg ctgccccggc ggagaccccg
     9721 aacaccccgg ctgagcccga aacccccgcg acggaagagc ccgccgcgga acccgaaacc
     9781 ccggccgcga ccggcagcgt gtactacctc aacttcaagc ccgagtctga cgcggcctgg
     9841 caggagattg ccaagaccta caccgagcag accggcgttc ccgtgaaggt cgtcaccgcc
     9901 gcttccggcc agtacgacac caccctgacc gccgagctcg acaaggacgc cgcccccacc
     9961 atgttccagg tcggcaacct cggcgctgtc aacagctacg gcgagttctg ctacaatctc
    10021 gaaggcaccg acgtgtacaa cgagatgacc accaccgact tcaacctgaa gaacgaagcc
    10081 ggcgagaccg tttccatcgg ctactgctac gaagccttcg gcatcatcgt gaacaaggcc
    10141 ctgctcgaga aggccggcca ctccgtcgat gagatcacca acttcgagtc cctgaaggct
    10201 gttgcggacg acatccacgc ccgcgctgac gagctcggct tcgacgcttt cacgtccgcc
    10261 ggcctcgacg gttcctcctc ctggcgtttc tccggccacc tggccaacat gcctctgtac
    10321 tacgagttcc gcgatgacgg cgtgacggag cagcccgcca ccatcaaggg cacctacctc
    10381 gagaactaca agaacatctg ggatctgtac atcaccgata ccgcgaccac cggcgctgcc
    10441 ctcctgtccg ccaccggcga tcaggccgag gctgaattcg gcgaaggcaa ggctgcgttc
    10501 tatcagaacg gcacttggga gtacagcaac ctgacttctg ccgacaagtt cggcatgaac
    10561 ccggacgacc tcaccatgat ccccatctac tgcggtgtgg aaggcgagga gaaggctggt
    10621 ctctgctgcg gcaccgagaa ctgctgggcg atcaacaaga acgcctctcc ggaagacatc
    10681 caggccactc tggacttcat gtactgggtc gtcacctctg aggaaggcac caccatgctg
    10741 gccgagcagt tcggcccctg cccgttcaag aacgccaaga ccccggagaa cgtgttcttc
    10801 gcggctgcca acgagtacgt tgccaacggc aactacaccg tgacctgggc gttcaactgg
    10861 actcctgccg ttgacgattg gcgcgctgcc gtcgtcgatg ctctgagcca gtacaccacc
    10921 ggcaccggcg actggtccac cgttgagacc gcgttcgtcc agggctgggc tacccagtac
    10981 gccaacgagc actaagtcct ctgcggatat ccgctgagat aaattgacac acaatacagg
    11041 cggggcgggc gatca
//
LOCUS       DAWAAE010000061        10906 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2708_length_10906_cov_2.874113, whole genome shotgun sequence.
ACCESSION   DAWAAE010000061 DAWAAE010000000
VERSION     DAWAAE010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_10906_cov_2.874113"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2312 a         3197 c         3052 g         2345 t
ORIGIN      
        1 gtactatgcg gtgtttctga ttctggaaaa ggttttcctt ctcaaaaagc tcgaaaaagt
       61 gccggtactg gcgcacatct acgcgctgct cgttgccgtc gtcgggtgga tgctcttcca
      121 gctcaactcc gtcggcgagg tcgtgcacta ttggggggcg atgttcggcg caagaggcgc
      181 tgcggccacg gcagccgacg cattttacgc gcgaagctat gtcgtcattc tttttatcgc
      241 agccatcgca gcgacacccg ttccccaaaa gctctttcat cgcattccgc agagagtaca
      301 aaatgtactc acgccggtgc ttgtcgcgct ttgtctggtg atctctacgg catatctcgt
      361 ggacgcgacg tacaatccgt tcctgtactt ccggttctga cggaaggagg ccaaaattga
      421 tgtcaaaaac gaccaatcga attattaccg ccctcttttc cgtgtttatc ggcggatttt
      481 ttctgctgaa tcttcttctg ccggacaagg acttttccga aaaagaaaac cggcagctgc
      541 agacgctgcc gaagttcagc ttctcttccc ttttctccgg ctcttttgcc actcgttttg
      601 aatcttattg cagcgaccag tttgccggac gggatacatg gatcgaaaca aaagcgggcg
      661 cagagcttgc gcagggaaag cagcagaata acggcgtgtt tctctgtgac ggcggccgtc
      721 tgatcgagcc gttcgacgcg ccggaggtag acgtactcgc gcgcgccgtg caggcagcgg
      781 atttactcac ggacaccggt gttccggtct cgctcgcctt gatccccacg acggcacagc
      841 tttatgcgga catcctgccg gacggtgctc cgaacgacga tcagcaagcg gtgatcaata
      901 gcgcctatgc gcagacggct ctggaaacga tcgatctttc ctccaccctc atggcacaca
      961 gcagcgaata cattttctat cgcaccgacc accactggac gtccctcggc gcctattatg
     1021 ccgccaatgc cctgcgttcc tcctgggggt tgccggagat cgataaagat acactcacac
     1081 aggaaaccgt ctccgaggac ttctgcggaa cactctattc ctcctccggc tttttctgga
     1141 tcgctccgga cgagatggaa acgctgaccg cagcgccgga gaattcgtcc gttacccgat
     1201 acgaaacaaa cgggaccgaa gagacgctgc cgctctacaa ttatgacaag cttacgatca
     1261 aggacaaata taccttcttc ctcggcggga acattccccg cgccgttgta gatacaggag
     1321 cggacaatgc cccttccctg ctcattctgc gcgattccta cgccgattcg ctcgtgccgt
     1381 tccttacaga tgccttttcc gagatccact tgatcgatct ccggtattat accggctccg
     1441 tcaaggaata tattgcagaa aatgccatcg accgggtgct tttgctctac agtaccgaca
     1501 acttctgctc cgattccagc ttccgaattt tttaaagtca attcatatga aaaatgcggc
     1561 agcgatccca tcgagatcgc tgccgcattg catcttttag tcggtcaaac cgcgccgaag
     1621 aaaagctgcc cacacgggag tacatattca aggaactgcg cgtagagccg cggcatcggg
     1681 agccccgagg ctgcgggata catgaggaca aaatacgcag cggcgacgat gagccagatc
     1741 cacttgacct ttccggacac ttccccactc tcttccatgt gctgcaaaaa cagaatggcg
     1801 gcaagcacga caaacgggaa cgtagtaaag aaatgatact ggaacgtgca gcgcggcacg
     1861 agcgtccacg gcagatagtt tgccgcgatc gcaatgagca ggatatatcc ggcctgcttc
     1921 cagtgtgcag actctttctt tgtccgacgg aacaatgctt ctacggccag cagaatggct
     1981 ccgatagcgg agacccacca cacggccggg ctgccggttg aagagatgct ggaaacgcgg
     2041 ttttctccaa gcgaccggaa gtaaaaccac acggagcggg atgtgaacgg ccattcatac
     2101 catgccgact gacaggtgtg cgttgcagtc agatcgtgat ggtaatgata catggactcc
     2161 tgctgctgca gcacaagcgc tatgctgccg acgccgttcg gctttgttgc ttcatagcgg
     2221 aaaaacggaa tatacgaaag atagtagata agcgccggga tcgcgaggaa gaacacgcag
     2281 cagaaaccga ccgtcgccca tgtcctgcgc cagaactctt tgcgcgccgc agtatccttc
     2341 cggcaggcaa taccggtgcg tatcatgtgc gcgaagaaca tcacggcaag cccggctccg
     2401 gcgtacagac ctgtccattt ggatgccacg ccgagaccga agcttacgcc gcacgcgccg
     2461 agcggaagaa agagcttttt gagcggctct ttcatgtaat ccatctggat atagtctgtc
     2521 atgaacagaa ccatgagcag aatgaaaaac aggatgaaca catcgaccgt ggcaatacgc
     2581 gtctgtgtaa agtgcatcgt gtccagcgca aggagcgccg cggccagaaa cgccagatct
     2641 ctccggcgga acagccgttt tgccagcgta aaaaacaccg gcagcattgc cgcgccgaaa
     2701 agcgccggaa cgacgcgcca cccgaacggc ttcatgccga agagcacaac gccgagcatg
     2761 atgaggatct tgccgagcgg cggatgcgtc cattcgtata cgctcatcgt atggagaaac
     2821 tcatacgccg tgcgggcatg gtagatctcg tcaaagtaca tgccgttcag atagcttggc
     2881 tcatccggta ctgtgtccgg ctcatcgaga agcgccgcgc cctccgttgt tccggtggcg
     2941 atcacagccg gaaggcggtt gccggcggca tcgaagaagg cgatctcgtt gagagagacc
     3001 tttcccgccg tgcattgcag tgttatatac cgcgtgtgca tggcggcggt ctgcgtgcgc
     3061 caggtaaaca tggtcgcgta tttctgcgta tactccgccg tggagccgtc gtcgccggtg
     3121 aacaccgccg agccctcggc aatgttcccg ttcgtccata tttcggcaac atcatattcc
     3181 gcaccgagat cgatgcgtac tgccgcaccg ctctgcgctg tccacacgct cgtcgggaac
     3241 gagagcgtgc ccagattcag aagcgtgaat accatataaa tgagcgtcag cgcccacatc
     3301 aggcgcttat ctccacggtc caagcaggta ctgcgctcct tggaagaaaa aatttcctgc
     3361 acggctcgtc acctccgatc gttctgcccg aaaccgcgtt ccagacagac cttggctgta
     3421 tagaaaaacg tcaatacttc aaaaagcgag catacgccga tcacgatctg gtgctgctgc
     3481 gtgccgcgga cggctgctga ggcagcgttg gatacaacgt acatcgccgt tgtctcgttg
     3541 agcgcaagaa caacgctcaa aagcatggac gaaacgagaa gcctattatc ccgtgtggca
     3601 gcataagcga atgccagcag agccatcacg gggaacacat accgctcatg catgtagtgg
     3661 ccaaacgtga agatcatgaa catacagaac gccgcaggga tcagcagccg ctcgggacga
     3721 gcacaaagcg tctctccgcc ttctctccgg cgcgaaacga cctgcatcgc aacggagagt
     3781 acgacagcaa gcacgatcat gaccatgccg aaggctttgt acgggatccc cggcacaagg
     3841 gaaagctcgg cggatttcca gttcccgcca cagagcgcaa ggaaattgta tgcctccacg
     3901 ctcgcataat cgtatccgcc cgcggaattc aaaaaccgac cgacgaccca gaaaaagccc
     3961 tgatcgcccc ggaacggaag cgaaacggcg aagatcacga gaagcgccgc cgcaacgccg
     4021 ccggccgtgc gcagagcatc ctcctttttc cggatggaaa ggagatactc cgccgcaagc
     4081 acgggaccat agataagcgc ctgccacttt gtcataatag caaggccgta gatcgctccg
     4141 gcggaaatgc ggcgtccctc gaaaagttcg gaaaagctca aaaggagaag cagcgttaaa
     4201 atcgagtcga tctgacccca ggcgccggag agaatgaccg cggcggggtt aaagaccgcc
     4261 aggccgccga gatacagaca cgcgctttca gaaaagcccc gcttgcgggc aaagcgcatg
     4321 agaaggatcg cgatgccgac atccgcagcg taggccggga gcatataggc aaacaccgcc
     4381 gcagcagatc cggaaggaat acggaaaagg tccagcagcc aagtgacagc ggcgagaacg
     4441 agcatatatc ccggcggata atcgtaccac tcgtggccgg gtgcggtata aaactgtgcg
     4501 gtgccgttct gcacgatatc gcgtccccat gcctgccagc aggttatatc gtaatcatgg
     4561 ccgccgaaca aaagcgtcag aaccagacga agcgccgcgc cggtcaggac aaggaggatg
     4621 aagcagcgtt ttactgtttt cggatccgcg gaaagcgaat aaaaacgctc acgattccgg
     4681 taaaagcccc agaggaggac acacgcgagg acgatggcgc agacgataaa cagatgcaga
     4741 tacgaccgga gacggatcgc acgggcctcg tccgattcgt tgacggattt tacggccggc
     4801 tgcgccgctt catcgagcga gaccgccgac gctatctcct ccggatcgtt ggtttgggca
     4861 agctccgctg cacgaaaccg cgccgtaccg gcggatgtct cactgtaaga accgagccgg
     4921 agggcgaaat tgatatagct ctgctttgcg ccggtacgga caatcagtgt ttcgcgcgtc
     4981 cagtcaaagc tgccggtacg tccggcgctg acggcataag agccgtcaag cgtgtaatta
     5041 tcgaccgaaa gacacacacc acgcccgccg tggacgccct cggcggagat ctccgccgaa
     5101 aggcggtata ccgtattcgg ctcgacttgg accatttgac agacgcgcac atcgttttcc
     5161 gcagcggaag caatgacaag tgcgccattc tgcgtataaa cggaataatt ctccgcttcg
     5221 tatgccacgg tatcccaccc ctccggaagc tccggcaaag agaaatccgc attggataaa
     5281 agcgagccgt cggcaaaggc ggcgctttgc agcaccacgc agaggatcaa tagaaatatt
     5341 agagaaagaa tcggttttcg ccgcttcatg gaatggcctc ccgaaaggac tgtttcctgg
     5401 attattgatt aattgcattt taacacagag cgtccgctat tgcaaacggg tcataattcg
     5461 ctataatagg attatatcca caagaaaaag gatgacaacg gtatgcttgg aattttatgg
     5521 tttttcctgt ggcaaagcgc agggatcgcc gtttcgtatt atctgtttcc cgaaaagagg
     5581 atcgctgtcc ggctctggct cggcagcgtg atcggaacgc ttctctccat gtgggcgccg
     5641 atcccgtttg cgttcctccg gggcttcggc atcacggcgc acatcgccgc ggcaatcttt
     5701 gccttggcgc tgcacgcgct gtggttcgcg cggcggggga aaaccgttcc ctgcgcggag
     5761 gatgccgcga tagaccgccc tctcccctat ctgcttgttc cctttatggt acttgcgatc
     5821 tatctcgtag gctcgcatac gctgcaaaac gtggacggag cgctctatac cggacagtgc
     5881 acctacggcg atatggcgat gcatttaagc ttcatcacat ccatcgccgg gcagcagacg
     5941 ttcccgccgg agtataacct gcttcccgga acgccggtga gctatccgtt cctctgtgac
     6001 agcgtctcct caagcctgta ccttctcgga acgccgcttc gctgggcgta tatgatcccg
     6061 atgttctttg cctttgctca ggtgttctgc ggcatatggt ttcttgcccg ggaattttgc
     6121 cagagaaagt acgcgccggt gctcgctttt cttctcttct tcctcaacgg cgggctcggc
     6181 atgatctatt ttctcaacgg tgagtattcc ctgcatgatc tcttcaccgc gttttacaaa
     6241 acgcctacga atctcaccga aaagggcatg cgctgggtca acgtcattgc ggacatgctg
     6301 ctgccgcagc gggcgacgct cttcggctgg gcagcgctgt tcgctgtttt gtacctgctt
     6361 ttccgggcgg tatttcgcgg cgatacgcgg ctgtatctcc cggcgggcat tctcggcggg
     6421 cttttgccga tggtccacac gcattcctac tttgcccttg gcctgatggc ggcatgctgg
     6481 ctcgtgtatt ccgctgtgcg tgaccggctt tcccgcgaat ggtttctccg ttggctccgg
     6541 tttgggcttc cggcggtact gctggcgttg ccgcagcttt ttctctggac atttccttcc
     6601 gtcggcggaa atgaacactt cgtccgtgtc gtgatcgact gggtgaataa cggaaaagag
     6661 ccctggctct ggttctggat caaaaatgtc gggctcatat tcgttctgac gccgtttgct
     6721 ttctttgctg tttctaagga acagcgcgcg gcgttctccg gcgcggtatt tattttcgtt
     6781 gtgtgcgagc tgctcgtatt tcagccgaac gaatacgata acaacaagct cctgtacgtt
     6841 gcctatgcat tcggatgctt tgtgtgcgcc gacgcgctgg ccggttggct cggacggctc
     6901 cggagcccgg cagcgcaggg cgtgcttctc gctctcacac tctttatcag caccaacgcc
     6961 gccgtgttta cccttggacg tgaggttgcc tccggtatcc cgaaatacgg ttatgagctt
     7021 ttctcccgtg atgaggccgc cgcggcggag tacattattg agaacactga gccggacgct
     7081 cttttcctga caagggataa ccacgataac accgtagcaa ccctcaccgg gcggaacatc
     7141 gtctgcggaa gcggctccta tctctacttc cacggcctga attatcaggg gcagcagcgg
     7201 cttgccgaac agatgctcac taacgcggag gtgtttgaag caaaccgcga aagcgaagga
     7261 ctggactatg tgtacatcgg ctaccatgag cgcgcgctca ccggtgttat caccgattat
     7321 ctgacggaaa attattccat tgcgttctct gccggagcga tcacgatcta tgatctccac
     7381 gcggacgcga tagactgaac ggccgtccgg cagcaatgca tttatatctt tttcgcatat
     7441 acgaaaaccg cccgcggtac ctgccgcggg cggtttgaca tatttcagag aacgaagctc
     7501 tccagctgtg ccttcgacac aaagccgacg gaggttttga cggcttcgcc gtttttaaag
     7561 aggatgagcg tcgggatgct cgatacgccg aagcgcatgg caagatccat ttcctcatcc
     7621 acattgacct tgccgacctt cacttcggca tgctcgtccg caaactcgtg cagcacgggt
     7681 gcaagcattt tgcagggtcc gcaccagctc gcccagaagt ccacgaggac aggtttatcg
     7741 gcgtgcagaa cttcttcttc aaagttggct tttgtaatgg taatttccga catggtagat
     7801 cgctcctttc gatgaagtat taatagtttt cggcgtggat ctcaaaatag ctctgcggat
     7861 gggcgcacgt tgggcagatc tccggcgcgc tcgtgccgac aacaatgtgt ccgcagttgc
     7921 ggcattccca gacctttacc tcgctcttgg caaacacttc ggcagcttcc acgttgtgca
     7981 gcagtgcgcg gtagcgctcc tcatggtgct tttcgatcgc gccgacaaga cggaatttct
     8041 gcgcaagctc gcggaagccc tcttcctcgg cggttttggc gaagccgtcg tacatatccg
     8101 tccactcgta gttttcgccc tcggcggcgt tcagaagatt ctccgccgta tcgccgatgc
     8161 cgttgagctc cttgaaccag agcttcgcat gctccttttc gttgtccgcg gttttcagga
     8221 agagagcggc gatctgctca tagccctcct tctttgcttt gctggcgaaa aacgtatact
     8281 tgttgcgggc ttccgattcc ccggagaatg cggcgcggag gttctgctcg gtctttgtcc
     8341 cggaatactt gttcttttcc gacatttttt ctactccttt acaaagcgta ttttccgttt
     8401 cgtagtatgc ccagatatgg ccaaagaacc gccgaaaggc agttctttgg ctgatttttt
     8461 tattccgctt ttttcagctc gcaaagcgcc tcggcaatgc gcgcagcgca gcgcgcgtta
     8521 ttcagcgcaa gctgcacgtt cgtttcaaag ctctcgccgc cggtaagctc cttgaccttg
     8581 gcaagcagga acggcgtgat gttcttaccg tggacattcg cctcgtccgc ggcctgcagc
     8641 gccacgttga tgatgcgctc catatattca tgatcgagcg cgtattcctc cggcacgggg
     8701 ttgccgacga ggatgccggc ggtgaagccg atgccgcgct gcgcatggat gatcgaggcg
     8761 atctccgctt cgctctgcgc cgcgtagtcc acgccgaagc cgctcttgcg gcagaagaac
     8821 gccggaaaat cggcggtctg gttgccgatg acgggaacgc ccatcgtctc aagatactcg
     8881 agcgtgcgtc cgatgtcgag gatctgcttg gcgccggcgc agacgaccgt aacaggcgtg
     8941 tgagcaagct cctgcaggtc ggcggaaata tccatggaat cctgaccgcc gcggtgcaca
     9001 ccgccgatgc cgccggtagc aaacacgggg atgcccgcca tggccgcgat gatcatcgta
     9061 gaagcgacgg tcgtcgcgcc ggtgcggccg agagcaacat acaccgccac gtcgcggcgg
     9121 ctgactttgc cgatgttctc ggcgcggcac atgatctcca gctcctccgc cgtgagaccg
     9181 accttgaggc ggcccttgac gatcgcgcag gtcgccggaa tgccgccgag cgagcggatg
     9241 acctcttcca cgcggtgcgc aaaatccaga ttttccggat acggcatgcc gtggctgagg
     9301 atcgtgctct cgagcgcgac aacgggctta ttctcatcca gcgcctgctg gatctcggga
     9361 agaacgtcca gatacttttc aaagctgttc ataattcatt ttcctccaga attttattga
     9421 cgagctccgc gctcatcccc ggactgacgg taccggcgct ggtgacggcg atgatgcccg
     9481 ccgcgagagc ccagcgagcc gcccgttcgg ggggaaagcg gtgcaggtaa gcatataaaa
     9541 gtccggccat aaaggcatct cccgcgccgg tggcatccac catctggctc acgggacgga
     9601 gcgcggcata gaattttgcg ccgctgcggt cggcccagta gcagccgcgc tgtcccatgc
     9661 tgacgacgat gcagtgcgta ccgcgctcga gaagcacatc cgcagcgcgc tccatatcgg
     9721 cgtcgccggt gatctcgcag ccggtcatag ccgcgagctc cagcacattg ggcttcaggc
     9781 agtggagctt tccggcaaac ggggcaagac gacgggcata cgttgtggaa accgggtcgg
     9841 aaaaaatcgg cacgcccgcg agcgcgtcgg aggtgatcca ttccagagcc tccggcgtca
     9901 ggcaggggtc acagacgatc gcgtccgctt tccgcagcgc atccttttgc ccttccagcc
     9961 agtcgggggt gatgcgctcc agaatacgca tatcggacat gccgatgaac atgtcgccct
    10021 cggggtcgat gagcgccata tagctggagc tgccgcagtc cgggcagcgc agcatgtgcg
    10081 acatatccat gccgaccgcc tcgccggagg cgaggatctc gcggccgtaa agatcgttgc
    10141 cgatggcgga gaacagagcc gacctctcgc ccatgcggga gagattttcg agaatattcc
    10201 gcgtgacgcc gccgggcgac gtgtggagaa agccggggtt ggaatcatgc aggatcagcg
    10261 cggcacggct gcggccgtgc agatccatgt tggccgcgcc gatgcccgcg gtaaagcgtt
    10321 tttctgccat tatttcaaaa tcactcgctt gaagttcttc ttgccgcggc gcaggacaac
    10381 gcccttgcgc agttcctcac cgtcaaacgc tttggcgatg tcggtgacct tttcctctcc
    10441 gcacaggacg ccgccctgct ggatgttgcg ccgcgcgtcg ctgcgggaag cgcagagacc
    10501 gctcagcacg aggaggctca taatgtcggc cttgccgtct acaaggtcat tctcggaaat
    10561 gacggcttcc ggcacatgct cggtgctgcc gccgccgaaa atggcgcgcg ctttttcctg
    10621 cgcggcgccg gcctcgtcct caccgtggac cattttggta agctcgtagg ccagagtttc
    10681 cttggcggtg ttgagctgcg cgtccttcca cgtcgccatt tcgtcgatct gctcgagcgg
    10741 gaggaacgtg agcatacgga tgcacttcat aacgtccgcg tcgtccacgt tgcgccagta
    10801 ctggtagaaa tcgaacggag aggtcttgtt cgcgtcaagc cagacggcgt tgcccgccgt
    10861 tttgcccatc ttcttgccct gcgagtcggt caggagcgta atggtc
//
LOCUS       DAWAAE010000062        10795 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2744_length_10795_cov_2.972160, whole genome shotgun sequence.
ACCESSION   DAWAAE010000062 DAWAAE010000000
VERSION     DAWAAE010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_10795_cov_2.972160"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3646..3655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5283..5292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6752..6761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8472..8481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2499 a         2823 c         3186 g         2247 t
ORIGIN      
        1 aaaaatgctt tgaactttat ttgcgcctgc gggcgcaaac tctgcgaggc ttttttgaca
       61 gtctggcgcg gcgggagcct tcccgccgcg tttttatgaa atgaaaggat agtaaatgga
      121 cgaacgtttc cgccgcgccg gggcaatcct cggcgacgcg gcgatgaaaa aactctccga
      181 aagccgcgta gcggtgctcg ggctcggcgg cgtcggctcg tggtgcgccg aggcgctctg
      241 ccggagtggg gtgggaacgc tgtatcttct cgatcaggac accgtgtcac tgagcaacgt
      301 gaaccggcag cttttcgcgc tcacgtccac gcttgacctg ccgaaagcgg aagcggcggc
      361 ggcgcggctg cgcgatatcg ccccggaaag caggatcgtc ccgatggtct accggtattc
      421 gcccgagacg cgcgatacgc tgcttgatct gaagccggac ttcatcgtgg actgtatcga
      481 tctcgtatcc tgcaaggtcg atcttgtggt gagctgcgcc gagcgcggca tccggctcgt
      541 cgcggcgctc ggcaccggca ataagaaaga cgcatccctt ctccggctga ccgacctcgc
      601 caaaaccgag ggatgcccgc ttgcgcgcgt cgtgcggcgc gagctgcgcc gccggggcat
      661 cacgcacctg ccggtcgtat attcgcccga ggaggcgtct gagccggata ccggtgaaac
      721 gccgccgccg ggccgccgca gcgtgccggg cagcctcgtg tgggtccccg cctccgcagg
      781 gttgctgctc tgccagtatg ttgtcacgga gctcataaaa taaaaaatcg tctctgaaaa
      841 accagagacg attttttcat ccgttatagc ggttcatcgc gcggtcgatg ccctgctgca
      901 cgcagcattc gatggcatcg gcggcgcgct cttcggcctt ttcgagcgct gcggcatctt
      961 cgccgcgggg aacgcccatc acccagtcgg ccatgtcgta atcagggtgc ggcggggagc
     1021 cgacgccgac acgcagccgg gggaagtttt ccgtgccgag atggagaatg atgtttttga
     1081 gcccgttgtg ccctccggcg ctgccggagg cgcgcatacg aagccgcccc ggtacgagcg
     1141 cgacctcatc ggaaacaacg agaacacgct ccggagggat cttgaaataa tccgcaagcg
     1201 ggcgcaccgc ctcgccggag agattcataa acgtctgcgg ctttacgagc agcacatcgt
     1261 gcccgccgat gttcgcgcgg gccgtgagcg cgcgcgaacg gaggcggttg atcgaaacac
     1321 cgcagcgctt ggcgagaatg tcgccgacgg caaagccgac gttgtggcgt gtgccgttat
     1381 accgcgcccc ggcgttgccg agaaagacga tcatccattc ggcgccgccg gaggaagaac
     1441 gaaacagcat agggatatct tactcgaaca gcggggaaat ggaaacttcc tcgtaaatgc
     1501 ggcggatggc ttcggcgaag agacggtcca cggagacata gttgatcttg tcgagacgcg
     1561 gctgatcgac ggggtaggga atcgtgtcgc agagcttgag ctccttgatg gggctcgcct
     1621 caatgcgttc aagcgccggg gcggagagaa cgccgtgcgt ggcgcaggca tagatctcct
     1681 tcgcgccgcc gatctcgatg agcgccttgg cagcgccgca gagggaaccg gcggtatcga
     1741 ccatgtcgtc aagcagaatg acggtcttgc cttccacgtt gccgatgatg ttcatgacct
     1801 cggatttatt ggcgacctcg cggcgcttat cgacgatggc aaggttcatg ttgagcttca
     1861 tggcaaactg gcgggcgcgg gccacgctgc ccacgtcggg ggagacgacc atcacgtcgg
     1921 ggttgccctt gccgaacttc tgcagatagt aatcggcgaa gaggggagcg ccgcgcaggt
     1981 tgtccacggg gatatcgaag aagccctgga tctggttggc gtgcaggtcc atggtaagta
     2041 cgcggtcggc gccggcggcc tcgatgatgt tcgcaacgag ctttgcggag atggggtcgc
     2101 ggctcttggc cttgcggtcc tgacgggcgt agccgaagta cgggatgaca gcggtgatac
     2161 gaccggcgct ggcgcggtgc atcgcgtcga tcatgacgag cagctccatc aggttgtcgt
     2221 tcacgggctt gcaggtagac tgcacgatga acacatccgc gccgcggacg ggctcaagga
     2281 tggaaacgga gcattcgccg tcggcgaagc gcttaatgtc ggccttgccg aggggcttgt
     2341 agagatggtt gcagatgctt tccgccagag cgggattgga gtttccggag aataccttga
     2401 cttctttgcc gtgtgaaatc aaaacaaatc acctttctgt ctttaatttt cgtggcgtac
     2461 ccggtgcggg cacatatttt cacaataagt ataccacatt ctccgaggaa agaaagcgga
     2521 aagttcaact tttcgagcct ttacatttgt ggtttttttg tatataatat cttagctaat
     2581 ttgatttgga atgcaaaggg aatttttgtc atgtcaatgt tggaatttga agagtataaa
     2641 ggcaagctca acacgctcaa gcctgcgctg gacgatctgc aggacgcgct ccatctggaa
     2701 gaggcgaagc gcgagatcgc cgagctgcag gaggagagcg cgcaggacgg cttctggaac
     2761 aacgtctccc actcgcagca ggtgcagcag cgcatcaagc agctgcagac caagtgcgaa
     2821 aagtacgaca agctctgctc gtcctgggac gatcttttca ccatgtgtga aatggcgctc
     2881 gaagaggacg atgcctccat gcttgaagag ctgaaagcgg agtacgatac gctctcggaa
     2941 aagctggaag aaatgcgtct ttccacgctg ctcaccggcg agtacgacgc gaacaacgcg
     3001 atcctgacgt tccacgccgg cgcgggcggc accgaagcgc aggactggac gcagatgctc
     3061 taccgcatgt acaccatgtg gaccgaccgc cacggctata agtatacgct gctcgactat
     3121 caggacggcg aagaggccgg catcaagtcc gccaccatca agatcgaggg cgacaacgcc
     3181 tacggctatc tcaaaagcga aaacggcgtc caccgccttg tgcgcatctc gccgtacgac
     3241 gcctccggcc gccgccacac gagctttgcc gccgtggagg tcatgcccga gatcgccgag
     3301 gacaacagcg agatcgagct gcgcgacgac gagattaaaa tggacgtgta ccgctcctcc
     3361 ggcgcgggcg gccagaaggt caacaagacc tcctccgctg tgcgtctgac ccacctgccg
     3421 acgggcatcg tcgtctcctc gcaggtcgag cgcagccact tccagaatct cgaaaactgt
     3481 aagtccatgc tccgtgccaa gctcgccgag atcaaggagc gtgagcatct cgaaaagatc
     3541 tcggacatca agggcgtgca gcagaagatc gagtggggca gccagatccg ctcctacgtg
     3601 ttcatgccgt acacgctcgc caaggacacg cgcactggct acgaannnnn nnnnngttat
     3661 ggacggcgac atcgacggct ttatcaacgc ctatctcgca atgaaggccg aaaaataaat
     3721 gcccttcgac cgcccgcggc gtcatgctgc gggcggtcgt atgcttttgg gaggaaaaac
     3781 gatgatctgg tatcgtatcg gagagtctct tgtacgtacg gaatccgcgc cttgtgcgga
     3841 tgtcccggcg cttgtgctgc ttacggcgga ggagctggag aaacgcccgg tccttccggg
     3901 gctggaaaaa gttctggagc atacgccatc ggtgcgcggt gcgcggacct gccgtgccga
     3961 agtgcgcccc ggctgtctgt ccgggacgct tgtgctcccg cgtaagggca aagacggtgt
     4021 tcgcgccgca ttcggtctgc tagttacgca aagccgcatc gttctcacgg atgattccgg
     4081 ctcgcttccc ccgctcctgc accgcattgc gcaggaaaaa cgatggacaa agggaacggt
     4141 cgggcaggca ctctatgatt tttttgaaca gctcattgcc catgatctgc atcgtttgga
     4201 agagctggaa gagcaggcgg aagccttgga ggatcaggtg ctcgccgatg gcgtcgaatc
     4261 tctcagcggc ccgatgaatg cgctgcgaaa ggagacaatg gcgctctttc gctattattc
     4321 gcagctcgac gatgtggcgt gcgagttgtg tgaaaacgag aacgggttct tttcggaaac
     4381 ggaaacagaa cgttttcacc tttttgagga gcgtgttgcc cgcctgcggg agaacgcaca
     4441 gctcctgtgt gaatactgcc ggcagatcca aagcgcgttt caggcgcaga tcgatatccg
     4501 gcagaaccgt gtgatgcaga tcctcaccat tgtcacgacg atctttcttc ccctcacgct
     4561 gctcaccggc tggtacggca tgaactttgt caatatgccg gagctgagct ggaaatacgg
     4621 ctatctcatg gtaggcgtca tcagcgcggc ggttttggtt ctcggtcttt ggatctgtaa
     4681 gaaaaagaaa ttctggtaaa gcagagagca agccggcggc cggcttttat cggctgccgg
     4741 tttgctgttg gcaaaatagt acatccataa tataacatat gttatacaac ggacgccgca
     4801 aggagaaaat agtatgctgc ccggcgttaa attccccggc gaggagatcg tccgcgccgc
     4861 gcttgcgctc gtccgcgagc aggggggata cgctccgtgt ccgcgctgtg cattcttacg
     4921 cacggcgtgc tgaacgcgct gagcgtgttc gctccggaac cggactacaa aacgcggtgc
     4981 gttatctttc ggatccccat cgttctcgcg gctgcgtacg cggcatatct cctgcggcgt
     5041 ctgccgccgt tgacaagcgg ggaaagctgt gtcagaatgg aaaaaaacac cgagggggat
     5101 taagccttga acgctctcga gctcgatacc gtattgctct caccggaccg gacgtgtctg
     5161 gaaattctgg atcagacgct gctgcccggc gaggtgaaaa cgctgcatct ttcggatatg
     5221 cgcgatatct gggaggcgat ctattcactg cgtgtgcgcg gcgcaccggc catcggcgtg
     5281 tgnnnnnnnn nncctgagcc agctctacca cgagcacaag cgcggcgtgt gcgcgggcta
     5341 tgcgctcgcg ctggccgcct cgcagatcga aacggaggat aaggatatct ttttcgcgcg
     5401 cctgcgtgaa acgaaggaat acctcgcctc cgcccgcccg acggcggtga atctcttctg
     5461 ggcgctcgac cggatgtggc agacggcgga ggcgcacgcc ggtgagagca ttccggccat
     5521 ccgcgagact ctttttgccg aggcgcagcg catccgcgat gaggacgttg ccatcagccg
     5581 ctccatcggc gagctgggct ttgctcttct gcaccacggg gacggcattc tcacgcactg
     5641 caacgccgga acgctcgcca ccgcgaaata cggcacggcg accgcgccga tgtatattgc
     5701 gcttgagcac ggctggaatg atcttcgcgt ctactgcgac gaaacgcgcc cgctattgca
     5761 gggcgcgcgg ctcacggcgc tcgagatgca cgctgccgga ttggatacga cgctcctttg
     5821 cgataacgcc gcgtcgagcc tgatgcagac ggggaaagtt aacataatat ttgtcggctg
     5881 cgaccgcgtg gcgcggaacg gcgacgccgc gaacaagatc ggcacctccg ccgtggcgat
     5941 attggcgaag cattacggca tcccgtttta tgtctgcgcg ccgagctcca cgatcgatat
     6001 gtcgctcgcg tccggcgcgg agatccccat tgagcagcgc gccgcggagg aggtcacgga
     6061 gatgtggtac aaaaagcgca tggcgccgga gggcgtcggc gtgtataacc ccgcgttcga
     6121 cgtgaccgac cattcgctca tcaccggcat catcaccgag cgcggcatct gcaccgcgcc
     6181 ctttgaggac gccttccgcg ctctcgggtt ttaagcggag gatgctttta tgacgggacc
     6241 cgatccaaac agcacctatc ccaacgagaa catccgtcag gtcgtgtaca taaaaaacgt
     6301 catttcccgg ccgaatatcc ttgtcgggga ttacacgtat tacgacgaca tagacggcgc
     6361 ggaaaagttt gaggaacacg tcacgcacca ttatgatttc ctcgacgaca ggctcatcat
     6421 cgggaaattc tgcgctatcg cccgcggcat tgagtttatt atgaacggcg cgaaccaccg
     6481 catgaacagc gtaacgacct accccttcaa catcatgggc ggcggctggg agaagtttgc
     6541 cccaaagctc gatgagctgc cgctgaaagg cgacacggtc gtcgggaacg acgtgtggat
     6601 cgggcagaac gtaacggtgc tgccgggcgt acacatcggc gacggcgcta tcatcgccgc
     6661 caactccgtt gtggcgaagg acgttccgcc gtactgcatc gcgggcggca atccctgccg
     6721 ggtcattcgc cgccggttcg acgaagagct cnnnnnnnnn ncggcgtatc ttctggagct
     6781 ccaatggtgg gattgggacg cggaaaaaat cttccgcaac atggaagcac tctgcagcgg
     6841 cgatcttgag aagatccgga atataaaaga gtaaaagaaa gcaagcaggc cccggacatt
     6901 tcgatgtccg gggcctgctt tgctttgacg ggaaactgga agggcaatac atgcaagtgt
     6961 ctgctttact caatttcctg ctccagatcg gtaaacaggc ttgacgcaaa aaaatcctgg
     7021 atcgtgatgc ctaagccatc gcatagcttt ttgatcgtcg aaatcgttgc gctgttgttg
     7081 cgcccgctga cgatgttatt gacggtcgat tgcgtgacgc cgctcatgtt gcacagttta
     7141 ttgatggaaa tgtcccgttg accacacagc tcgatgatcc ggacccgaac cgcttctcca
     7201 atgttcatgt acatgccccc tttacggaag agcatatcca gaagtacttg aaaagattaa
     7261 ctcttttggg ttataattat ctcaaatgag ttaattctaa aacatggact acggcgaaaa
     7321 aatgtgtaag gagtgcatcg agaatgaaag aaaacgaaag gctgctattg ttttatgagg
     7381 aatgcgtgaa aaaaggctat tccgacatga cagacccgca gcaggcgctg aaagcaaagg
     7441 ttatcgccac tgacagaaag ctgcggtacg gaaactccat agagcagttc ttccagaaag
     7501 cgaaagcggc gtataaagaa cgtgaggcag aacggattgc agagaagcag aggatcgcgg
     7561 aggaaacgcg gcgctgtgcg gtgccgggag aagagctggt aaagctccat agcgccccgg
     7621 aggtgatttc cgtttttaaa cgaccagatg gaagcttcta ttgcacgttc gataagaatc
     7681 cggaaagaaa aatagagggc aaaccggaat ttcgtgttgt gcccagttct acggtttcct
     7741 atgagtatca tccgtcggag acgagaatta ctatggcatc gaaaggcggt tttaccgacg
     7801 gacaggtgtg ggaaacggat ccgtgggttt cgagaaaaca tgaacatacc ggaatggcgt
     7861 ttttagagat aactgttaac gaagaatcgt gggaagtata ccaaatcaca ttatcgcctc
     7921 ttgttaagga atcttttaag agatatccct gttatgacag ttccttaaaa cagaagttaa
     7981 aatctgggga aatccaatgc catgtaaatg tcagagattc tatgtattat tcggggttca
     8041 agcaggcgtt aggccagaat gatatgtacg ctctcgaatc gagtgcatct ttcgttatgg
     8101 atgaggaaag gttgccgatt ggccaaataa cggtaattaa aaagctgctt tgcaagatac
     8161 tcgatggccc atggccggag aatgacgaac aggcctatgt gcgggggata aagctgatag
     8221 aatccgataa gtcatcggaa attctcgagg ggataaacct tctgcaggag atcggtgaaa
     8281 accaccctcg aaagagaagc gccgaggagc tgattgaacg aaataaagat agatatgaag
     8341 ctgtattaca gagggaaaaa gaggcagcga taatcagaaa ggaaaacagg cagcgtaaaa
     8401 gatacaaggg gtttgttata gtttcatcca tcgcatgcgt cgctattata cttgtaggaa
     8461 ttatcacgca gnnnnnnnnn naaaaaagca ataagcttga ttgacgccgg agagtatgat
     8521 gcagcttatg caatactgga ggaaattgga aatacggagg cgatagttgc gaacaagtat
     8581 aaccgtgcaa tggaatgcat agacgcacaa aattatgaaa cggcatatat gcttttggat
     8641 gggctggatt accaagacag cgcccaaaag cggaaagaaa tcaaatcgca gtatcatagc
     8701 gcggttctta tgaagacaga tgtgggcgga actgttttct tcggcactta tgagcaggac
     8761 aacaatgcat ctaacggaaa agaagatgtt gaatggttag tacttgaaaa aaaggataac
     8821 cgccttttgg ttgttagcca gtacggtttg gatagtcaaa cgtataacac tgggaaaaaa
     8881 ggagccacat gggaagcctg cgcacttcgg caatggctca ataacaactt ctttaattca
     8941 gctttttctg acgatgaaaa agcaatgatt ccagaagtaa ccgtattggt ggacgaaaat
     9001 ccaaactatg atacagatat cgatactgat acgcaggata gagtattttt acttggtatt
     9061 tccgaagtaa atatgtactt tcattcagac aacgagagag aatgcaaacc tacaaagtac
     9121 gctctatcaa aagacgtgag agtatatgac agcggaaact gtgcatggtg gttgcgaaca
     9181 cctggcaatc cagattctgc ggcaatggtt catggagacg gttcgatttg ttgtcttggt
     9241 ggcagtgatg ttatagttgg cgccagcgtt ggcagcaatc tggccgtccg acccgccatg
     9301 tggatcaatt tgagtaatta aaaaggctcc cttgtgcaaa aggagttttc agcgaagctg
     9361 actgagggat cgtctcaaac tccgcgtaga ccgaccgtcc ctctgttacg gcgttatgcg
     9421 ccgtaaagcc gtcccttacg caaggaactt tataaaacgt aaaagcagca gctcgtcgga
     9481 gctgctgctt ttgcatagtt aatggatcac tcgctcagcg gcgtgatgcc gcagctggtg
     9541 aggaatgtat ccgaaagggg agagccgaac cagttataga gccgccgggc gggactgtcc
     9601 tccggctcgt cgctgcgaat gacgacatag cccttcgtgc cgagcgggta atcgccggtg
     9661 cggacattct ccggcgtggc gctgtagccg ttcagcgaga aggcgagcag cgcttcgttg
     9721 gaatagacgt cgttcagata cgtcataatg ctgtacccga tggcgtaagg atcgtcgaga
     9781 caggcggaga cgagcgtgtt catctcgtcg agccgctcaa agccgagcgc ctcgtaatcc
     9841 ggaacatcct cgcctttgaa caccatcttt tcaaaaagcc gctggctgcc ggactgatcg
     9901 tcgcggtaaa gcacgcggat cgcgtgatcc gcgccgccga gctcgctcca gttggtgatc
     9961 tcgccgcgga aaatggcgcg tacctgatcg agcgtcaggt cggtgacgcc gcatgcgctg
    10021 ttcccgatga acaccagccc gtcgccgccg tagagcttca tttcgacctc cacgccccgc
    10081 tcggcgagat aggccttttc ttcatcggtg ggcgcggcga gcagggcgat gtccgccttc
    10141 ttatcggcaa tgttcagcca ggcgccgtgc gttgtggaac agagcggctc cgcgccgccg
    10201 tagccggtgt caagacagaa gaagcggtag atgttctgca ccaacggctt tctcgctgta
    10261 gaggagtcca taaccgggaa atcgccgtcg gcaaacagtt cgctgtacag accgtttaga
    10321 tcgacaatgt acagtttggc cgattccata tcaaggacaa tgtacgttgt tgtgtttgtc
    10381 cagccgtaat cgccggcctc aacactggcg ctgcacgaga tcagaacctt gccgtcgcgt
    10441 gcagaggcga cataacgggt ggtttcggcc tgcgggagat ccagcgtcag tcgctcgctg
    10501 ccgcagacaa gattgagcgc aaagtttgtc tgatagtttc cttcgacgag gtaggtgtcg
    10561 ttgtaggcgt gtagaatgtt gtcgttcaca gtaaaatatc cgctcaaaat actgattgca
    10621 ccattgacag ggtcgacgcg catctgaaca ccgcggctgc tgtcactgat gaagtagtta
    10681 tccgaggagt tgtaagacgg atagcagtac gggtccaaaa tgtctgtcca cagcttttcc
    10741 aaagaaggaa gtacatagcc ggtgatgcgg caggtatctc cggagtaatc actgc
//
LOCUS       DAWAAE010000063        10695 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2773_length_10695_cov_3.508553, whole genome shotgun sequence.
ACCESSION   DAWAAE010000063 DAWAAE010000000
VERSION     DAWAAE010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_10695_cov_3.508553"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1118..1127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6301..6310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7412..7421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2134 a         3213 c         3361 g         1957 t
ORIGIN      
        1 cgcgtggtgc ccgtactgcg gcacggtgat ccggcagcag gcacaaaact gggccatcag
       61 cgccattcgc ggcatccggc aggagaccgt ataaaaccgc cgcggcacag accgcgaaag
      121 tgaggggaaa accatgcttg acgttgccgc gctgggcgag ctgctgatcg actttacgtg
      181 cctttcgacc gacgaaaacg gctatccgac catggcggcg catcccggcg gcgctccggc
      241 aaactttctc gcggcgctgg caaagtacgg cgcaaagacc gcgatgctcg gcaaagtggg
      301 ggacgatacc tttgggcatc tcctgctcga tacgctgcgg cgcgcaggca ttgagacgcg
      361 cggcatgatc gccgcgcccg atgtgttcac aacgctggcc ttcgtcacgc tggacgcgcg
      421 gggcgagcgc gagttcgcct ttgcccggaa gcccggcgcg gacacctgcc tctcgttccc
      481 ggagatcgat ctttctctca tcgatgaggc gagggtcttt cacttcggct cgctgtcgct
      541 gaccgatgag ccgtcacgct ccgccacgcg gcaggccgtc gcctacgcga gagcgaaggg
      601 gaagctcgtc acctatgacc cgaatctgcg tccgccgctc tggaagaatc tcgcggaggc
      661 aaaagagcag ctcctctggg ggctggggca ggcggatgtg gtgaagatca gcgaggaaga
      721 ggtgcgtttc cttttcgggc tggagccgga agcgggcgcg gagcatatct gccatacctt
      781 ccccgtgaag ctggtgttcg tcacctgcgg cgcggacgga tgctatttca aaaactcgcg
      841 cgccgcgggc cgcgaaccgg cgctgccggg gctgaggatc accgatacca ccggcgcggg
      901 cgatattttc ggcgggagcg cggtatggaa gctgctgcga tatggcgtcc cgccggaaga
      961 gctgggtgag agcgcgctgc gagatatcgt ctcctttgcc tgcgctgccg cgggactttc
     1021 cacgacaagg ccgggcggca tttcgagcat cccggagcat gacgaagtcc tgcgggagct
     1081 gaaccgataa aattgaacca gagaatcaaa aggcattnnn nnnnnnncta acgcgtggtt
     1141 ttcgggtata caagaagacc accggcttgc gccggtggtc ttgccttccg aggagcggat
     1201 cagcagtagt actccatcgg gtaggtgagg tattcgaccg gctcgagctg gtcgcaggtg
     1261 acgtatccgg cgggagcgcg gagcagggac gggatgcggt tgacgaccgt ggcgcaggtg
     1321 tgctcgacgg tcgcgggctt cacgacatgg aactcggtct tgggctcgcc gaagatctcc
     1381 caatcgcaca tatcgccgtc gtccgggccg tagaccttgc cgatggtttc ttcctcgacg
     1441 gtgatgccct gcatggtctc gatggtgacg accgcggaca tgccgatggc gtggccggcc
     1501 ttgatgtcct tgccgagcgt ttcgctgtaa atgtccttgt cgatgatgca gggaacatgc
     1561 ttctgggaaa tgctcttgat ggtgagaccg agcttattgc agatggcttc ggaggcgttc
     1621 caggcgtagg acggctcaaa gtcggtcggg tgagcgatct tcgcttcaaa ctcttccggc
     1681 gtcaggccgc agccgtgggc ctcggcgagc gcgatgccgt actcgtccac gttgtagctg
     1741 taagcaccct tgatcttcgt gatgttgtgg cagcccgccg caacgagacc gaccatgttg
     1801 atccagaaga tatcctgcat gccgctgccg gtgatggtgc agccgttttc cttcgcaaga
     1861 gcgtcaagct tgttgatcat ggccgcagag gtcgtccagg ggtaaatggc ctcttcgcag
     1921 gtcgtgatga cgttgatgcc gcgggtaacg cactgctcga tggcttcgta aatgtcgccg
     1981 atgaagctgc gggtcgtgat gatggcgacg tcggcgtcgc actcgtcgag gaccttctcc
     2041 gcgttatcgg aaatgatgac gcccgtcttg acgccgagct ccgcccagtc gccgatgtcc
     2101 tggccgacgg acgcgccgtg gccgatgccg ccgacgatct gcgcgccatg ctcatacgca
     2161 tagcgaaggg tgtacttgct catctttccg caaccatact gcacaacttt aattttatcc
     2221 attataatag ctcctttctg tgctataaaa tactataatg gagtatacag tctgttgctg
     2281 gtagagagtc aagagttttg ttaattaatt ttttgtgaag atcacgggga cctgcagacg
     2341 caggaacatg ttgcgcttct tattatcgaa aagattcagc tccgtcatct cctcacagat
     2401 atagtcgccg ttcaaaatat agtgattggc gcggcagtat gcaagaaggc gctccgcgcc
     2461 ctcggcctcc tcgtcgaagt catcgagata ggcgcaggca aacatgccgc tttcgatgac
     2521 ctcgatgctg tcaccgtaat cgcgcaggtg cttgtcgccg aagatgaaga tcttgtccgc
     2581 gatgagcgcg ccgcgcgcga aatcgtcctg tttcacgctc gttccgagac tgtacgacaa
     2641 aagctgcggc agattgcgcc ccatcagctc ccggcgcagc gcgaggacgg ccttttcgta
     2701 ggcgacgatg ccctgctcgt agaagttttc ggcgcacgga atgtacagaa tgtgacgctg
     2761 atcgatgaat tccagcgaga ttgttccgct gaccggcgac ttccggtagc gctcgatgga
     2821 ctggatcgtc cgctccacgg cctcgtgcat cgccttgaga tcgcgcatct gctggtggat
     2881 ctggttgttt ttttcaatga gcttctgctc gatgagcatg atgtcctctt tctcgagcag
     2941 cgtctggata tcggtgaggc tcatgttgag attccgcatg taccggatga tatcgagccg
     3001 cgaggtctgg cggatgtcgt agtagcggta attcgtctcc tcgtcccggt agctcggctg
     3061 caaaagtccc atccggtcgt agagtctgag cgtggcgatc gaaacgcggt tcgcctccgc
     3121 catttcgccg atagacagca gatgcgcgcg tttttcgtgg tccattttcg tccctccgtg
     3181 tggcaaaaaa ctctaatcct gctgtagact tttgtacccg aaaacggcgc gtttgtaaag
     3241 cccctttttc tctttcaaca aagaaaaacc ggagccggag agatcgtctc tccggctccg
     3301 gcgtataaat aatactatgt atcagggtgc gtccccggcg aggacggcga gctcggcgcg
     3361 cagcgcgtcg taaaactttg ccatatggct gccgtcggcg agtgcgtggt ggacgagcag
     3421 cgtcaccggc atttgcaggc ggccctcggc gtctggcaca aacgcgcccc aggtgatacg
     3481 cgggttggat tcctcgccgc ccgccacggg ctggatgagc gcgctgtaat gcagccacgg
     3541 gagcgtggag ataaaataca tgctctccac gttcgcgtcc tcgcgcagcg agccgtccag
     3601 acggcggcgc tgccgctcgg tctcggcata ggcgatgtat tccgcaaagg gcttatcgtg
     3661 gtgcagcgtg cagtagcagt atgtcccgtc cgcgcaaagc tccgtgtgcg acgtcggaca
     3721 ggtatcgtat tcgatgatga cgccgtcatg gatgcgccgc cgcagctccg gcacgcggtc
     3781 ggcggccttc gccgcggcgt ggagaaacgt gagataaaac gaatacccgc gcgccttgca
     3841 gaaggatacg atatccgtta catcaatatc gaccgtcacg ccggcatagg ggtacggcat
     3901 cgagaggaaa taccggaagt gctcgatgcg ctcataatgc tccatgtcca gataacgaaa
     3961 gctcatacct tctccttccg cgcccgaagg cgcagcagcg cgcgtccggc gggcttttcc
     4021 acaagatacg tgagcgccgc ggagagcagg aacgcggcgg caaagcagag cagcgtgtat
     4081 ttcgtctgcc aggcgcgccc ctcgctcatc tgcgggaact cggaaacata cgccgggatg
     4141 tgaaactcct tgagcttcac ggcgagaaac tggtgccaga tataaaaatt ataggatatg
     4201 gcgctcaaaa accggacggc gcggtttccg aaaatacgat ccagacgctc cggcgcgcgg
     4261 ccggaaaaat agagaaacgc cgcgccgcac acgccgagcg gcagccgcca gagaagctgc
     4321 gccattttga tctccgcgtc ccccgccgcg gtctgccgcc agaggacggc aaacgcgccg
     4381 aagagcgcga gaagcgcgag cgggaagaac gcccaccggc gcggcgcgtc cgcacgcttt
     4441 tcaagaaggt gcgccgcgag catgccgagg gcatagagat cgagcataca gggaagctga
     4501 ttcagccaga gcgacagctc gtgcgcgcgc agcgcaacga gagcgcggct cacgagcgcc
     4561 gcgagcgtca taagcgagaa tgtgagcgcc ggacgctttc cgaacgcgcg ggcgagcagc
     4621 gggaagagga gataaaactg catctccacc gcaacggtcc agagcgcggc gttgaggctc
     4681 gtccagtaat acgtgtccat cgagaacgtg tgcgtgaacg tgagatgcgt gagaagatcg
     4741 cgccagagcg gagcgctccc gacggcgcgg ccgcgcgcga gcgcaaacgc cgccgcaacg
     4801 agcaccgcga gcagatagca cggcagtatc cggcagagcc gccggtaata aaaccccttc
     4861 acgtccgcga tccgtccgcc cgcgcggacg agcggacggt agagcagaaa gccggtgagc
     4921 agcagcgtga gatccacgag catgtacccg cgccgcacga tgcgctgaag atcgaaatag
     4981 tgtccggcga tccggaacga cggatcgagc cacgactgct gccagatatg gaaccacgcc
     5041 acggtgaaga tgcaaagaat gcggagaacg tccgccgcgg cgaagtaccg gcggccggcg
     5101 tttacaggga gagaatctcg catttgaact ccgttccttt cacggtgatc atgccgtagg
     5161 tctgccggcc catgccggcc gagccggggt taaaaagcgt tacatcgccc atgcggagaa
     5221 agtccgcctc gtgcgtgtgg ccgaagagcg cgagctgcgc gcccgaaaag tgcgcggcgt
     5281 tcgcaaggct ctcgtacccg agttttacgt tgtaccggtg gccgtgggtc ataaagatgc
     5341 gcacgccgtc ggcgttgaag agaaggttct cctccgcgtc gctgcgggaa taggcgtcgc
     5401 agttgccgcg cacataccgc aggtcgagcg acgggaaata cgccgcgatc gcctccgcgt
     5461 cgcgcacata gtcgccgaga tggagcacca tatcgggctt ttgctcctca atggcggcga
     5521 gcatcggctc cttcacgccg tgggagtcgg aaaaaacggc aatacgcata aagcactctc
     5581 tttctccccg ctgcatatac tccttacagg gggtggaacg gatgatggat atggaaaagc
     5641 tgggcgcgga gctgctctcc gggcggcagg gcggcgcgat ccgcgatatg gccaactcgc
     5701 ccgaggcgaa aaagctcggc gcggcgatcg atcctgccgc ggcggagcgg gcagtgcgca
     5761 gcggcgacgc cgcgcagctc aaggcgctcc tcacgcagat cctctcgacg aacgagggca
     5821 aagccctggc cgaaaagctc tcgcgcttcg aaaacgggaa atgagcgagc tggaagagcg
     5881 gctcaacgcc gtgctctcca atcccgagga gatgagccgc atcgcggcca tggcgcagaa
     5941 gctcatggga gagcttccct cagagagcgc cgcggcgcct tctgcgccgg aagcggcggg
     6001 cggcgggctg ctcggcagtc tcgccgggag cctgccgggg cttttgtccc gcctcacgtc
     6061 gggacagagc ggaaacggga aaaaacaatt ggccgacgcg ctcgcgccgt atctgagcgc
     6121 ggaacgggcg cgccgtctct cccgggcgct gcgcattgcg ggcgcggcgc gcatcgcggg
     6181 ggcggccttt tcggaatggg ggcgcggcga tggcctataa ccgctataac ggcagcaccg
     6241 gcgagcgcac gcgcgtggaa gagcctttgc cgccggaaaa ggcgcagagc ggctcgccgc
     6301 nnnnnnnnnn cgcgagagcg cgccggagcg tcccccaccg ccgccggaga acgaaaaaac
     6361 gccgccctcg ccgctgctgt ctcttcgcag cgggctggac ggcatcttcg agcggctcga
     6421 cccaaagcgg ctggagaccg aggaccttct ggtgctggcg atcttgtggc tgctctaccg
     6481 ggagagcggc gattgggaaa tgctcatcgc gcttggcgcg tactattttg atctgtaggg
     6541 acggctcacg gtttcgtgct gccgtcctct tttttgccct tctccggcgc cttcgccgaa
     6601 gggttggacg ggaaatgatg acccgcgaaa acatacagcc atatgccgag cgcgagcgcc
     6661 accgcaccga gcagcagcca gaagcgcatc gcggcgttcg ccgcgagcag caccgccgcc
     6721 gcgccgagaa tgccgctggc ggcatagagc acggcgaccg cctgcttctg cgtgagtccc
     6781 agcgcgagca gccggtggtg gatgtggccg cggtcggcgt ggaacgggct ctcgccgcgg
     6841 ctcacgcgcc ggacaacggc gaacacggtg tccgtaagcg gcagcgcgag cgtgagcacc
     6901 ggcaccgcga acgcgacaag cgtgtacatt ttaaaaaggc cgagcaccga cgccgtggag
     6961 agaacgtagc ccaaaagcag cgagccggag tcgcccataa agatcttggc gggattgatg
     7021 ttgaacggga gaaacccgag acacgcgccg ccgagcgcgg cgacgagcac ggagatctcc
     7081 gtcgcctgcg ggagaaagag cgacacggcg agcatcgagg cgcacccgat gccgcacacg
     7141 cccgcggcga gcccgtcgag cccgtcgatg agatttaagg cgttcgtcat gccgacgatc
     7201 cagagaagcg tcaccggcac gctcagtgcg ccgagcgcga tcgcctcggc ggaggcgtcg
     7261 ccggggttcg tgagaatgcg gatcacaacg ccgtgccgga tggcgacgat tgcggcgatg
     7321 atctgcaaaa cgagcttgac ccaggggcgg agcgaaacga gatcgtccac cgcgccggtc
     7381 acgacaatga tgacgctgcc gagcaatata cnnnnnnnnn ngatgattgt ggacgcgccg
     7441 ggactcgccc ggcatgtcca tcgcgccgat cttgcaggca aattttcgca cggcgggtgt
     7501 ggcaagcaga cacaccgccg ccgcggcgag cagcgcgagc agcacacgca cccgcagccg
     7561 cagagccaga gcaggaagca ttcccttccg cctttctttt tttcagacac cgccgcgcag
     7621 cgcggcggtg cctgaaattt actgaaaatt agatttggac gaagccgatc ttcttataga
     7681 ccttgcgcag cgtgcggttg gcggcacagg ccgcccgctc cgcgccgtcg cgcaggatat
     7741 cttcgagata cgccttgtcc ttcatgagcc gttcggcctc ctcgcggatg gggcgcagcg
     7801 tttccaccac ggcctcgccc acggcgggct tgaacgcacc atagcccatg ccgtcaaatt
     7861 cggcctcgat ctcgtccatc gtcttacctg tacagacgct gtagatctgc ataaggttcg
     7921 ttacgcccag cttttcgggg caggcataca cgcagtggcc ggtctcagag tccgtcacgg
     7981 cgcgcttgaa gcagcgcata atgtcgtccg gggactgcat gatatagatg cacccgccgg
     8041 ggtccttgtc tgacttggac attttgttct ccgggctcgt gagactcatg atacgcgcgc
     8101 ccgtgggcgg gatatagggc tcggggatgg tgaacacgtc gccgtaaatg ttattgaagc
     8161 gcatggcgat atcgcggcag atctccacat gctgcttctg gtcaccgccg acggggacaa
     8221 ggtcggtctg gtagagcagg atgtccgcgg ccatgagcgc ggggtaggtg aagagaccgg
     8281 cgttgatatt atccgcattt ttggaagact tatccttgaa ctgcgtcatg cgggagagct
     8341 cgccgaacat ggtgtagcag tcaagcaccc agccgagctg cgcgtgcgcg gggacgtggc
     8401 tctggatgaa cagagtgttc ttcttcgggt caagaccgca ggcgatgtac tgcgcgagct
     8461 gcgaaagcgc gcggcggcgc agatcggccg gcacctgccg gacggtgatg gcgtgcatgt
     8521 ctacgatgca gtagtagcat tcatattcat cggagagcgc cacccagttt ttgatcgccc
     8581 ccatatacga gccgagcgta agatcgccgg agggctggat gccggagaat atgatttttt
     8641 tgcgggcagc ttcttccatg atgattcttt cctttcataa gcattttaaa gatgcggaac
     8701 tatggtttcc gtacttttta attatggcat tataacacgc cggttttggc ttgacaatac
     8761 agagcctatc caataagatg ggaaaatgac aaaagacctt tgggaggttt atccgtatgg
     8821 acaatacatg gtttgagaaa atggaagagc gcatcccgcg cggcgaggta cgcacccgct
     8881 tcgcgccgag ccccacgggg tatatgcacg tgggcaatct gcgcacggcg ctctacacct
     8941 ggtgcatcgc ccgccacgcg cacggcaagt tcctgctccg cattgaggac accgaccagt
     9001 cccgccaggt agagggcgcg gtcgaggtca tttataaaac gctgcgcgac tgcggtcttg
     9061 accacgacga aggccccgat gtcggcggtc cggtcggccc gtatgtgcag acggagcggc
     9121 gcgacaccta tcttccctac gccaagcgcc tcgtggagcg cggccacgcc tattactgct
     9181 tctgcgacaa gaccgaatcc gaggaggatt ccggcgactt cacccgcaag gacgacccct
     9241 gccgcgatct ttccgcggaa gaggtgcagc gccgtcttga cgccggtatg ccgtgggtca
     9301 tccgccagaa gatcccccac gagggcgaaa cgacgttcca cgacgagatc ttcggcgaca
     9361 tcactgcgcc gaacgccgat ctcgacgatc aggtgctcat caagcgcgac gggctcccga
     9421 cctacaactt tgcaaacgtc attgacgatc atctcatggg catcacccac gtcgtgcgcg
     9481 gcagcgagta tctctccagc gcgccgaagt acaaccttct gtacgagggt ctcggctggg
     9541 acgtgccgag ctacgtccac tgctcgcccg tcatgcgcga cgcgcacaac aaaatgtcca
     9601 agcgccacgg cgacccctcg tacgaggatc ttgtcgcgca gggcttcctc accgaggcga
     9661 tcgtgaacta cgtcgcgctc ctcggctggg cgcccggcgg cgagcgcgag atcttttcga
     9721 tcaacgagct cgcggaggcg ttcgatatgt cccgcatctc gaagtccccg gccattttcg
     9781 atatcgaaaa gctcacgtac ttcaacagcg agtacatcaa ggccatgtcc cccgaggcgt
     9841 ttgcacgcgc cgccgagccg tggatccgca aggccgtcaa gaccgaaagc tgcgatcccg
     9901 ccctcatcgc cgccattttg cagcagcgca ccgaaaagct ctcggacatt cccgaaaagc
     9961 tcggcttctt cgatacgctg cccgagtacg acaccgcgct ctacgtccac aagaagtcca
    10021 agtgcgacga gaccgtcgcg ctcgatatgc tcaaaaagat gcgcccgcgt ctcgcggcca
    10081 tcaccgactg gacggaggag gccgtgcacg actgtctgat cggcgcggcg gcggaattcg
    10141 gctgcaaaaa cgctctcgtc atgtggccgg tgcgcatcgc cgtttccggc gaggccgtga
    10201 cgcccggcgg cgccgtggag atctgccaca tcctcggccg gaccgaaacg ctcgcgcgca
    10261 tcgacaaggg catcgaaaag ctggaaaaat gacaaaataa ggtcgaagaa attcggcaaa
    10321 acagcgcctc ttttcgccat actaagcgaa aagaggtgct gtattattat gaagaaacga
    10381 acgaccatac gaattatctc cttcctcgcc gccgcgaccg tcgcgctcgg cgcggcgagc
    10441 gtcattttct accgccgcgc cgtgaccgcc gagcgggagg agcgccaccg cggggaatac
    10501 gctttttccg agctgtgcgc cgcggcagac ggcctttccg cggcgctcga aaaatcacag
    10561 tacgccgtga gcccggccat caccgcctcg ctctgcgcgg aggtctattc ccgtgcgcag
    10621 acggcaagcg cggcactctc atcgctctcc gtcccgctca tggagctgga gaatacggcg
    10681 tcctttttcg ccaga
//
LOCUS       DAWAAE010000064        10689 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2774_length_10689_cov_3.492289, whole genome shotgun sequence.
ACCESSION   DAWAAE010000064 DAWAAE010000000
VERSION     DAWAAE010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2774_length_10689_cov_3.492289"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3546..3555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7840..7849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8423..8432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2179 a         3233 c         3003 g         2244 t
ORIGIN      
        1 cccgctttct gatggagatc gccgcggggc gctcgctcga gagcctgctc gaagggcttt
       61 acgagccggt cgagagccgt gagttccggc tgaagatcct tgcggaggac ttccgcgccg
      121 cgggcacagc ggtgcttgat tctcttgcgg agtacgcgaa ggagcagccc ggctggagca
      181 tcgcgccgga caactgcgag ggcgtgcgcg taaatctcga taaagcgcac ggcgacggct
      241 ggttcctcct gcgcctttcg ctgcacgatc cgatcctgcc gctcaatatc gagagcgaca
      301 gggagcacgg cgcgcgcacg atcgcccggc agctcgctcc gttccttgcg cagcaggtca
      361 atatcgacgc ttcctccgcg ctggaattta cattgtaaag ccgtgtaaag gatgtttgtg
      421 tttcccgcgt gaacaaacta cacaaagtgc tacaaaaatg cgctaatgtt ctattgcatt
      481 ttcccgatga agaggctata atgtaaggca caaattcccg tacaggggat taattttagg
      541 aggaagataa cccatgaaga aagcacttgc tctcattctg gctctggtca tggtcctctc
      601 gctggctgcc tgcggcaccc aggccgagac tccggccacc gagccgacgg aagcccctgc
      661 ggaagcgcct gccgagcagc ccgttgagtc cgaaaccccg aaggattact ccggctacac
      721 catccgcatc tactccaact ccaactccac cgagcgcacc acgtggctcg tgaacgaggc
      781 gaaggaagcc ggcttcacca tcagcattga cgacaacagc gtcatctccg gcgacacggc
      841 cgccatccag gctgccaacg agaagaagga cggcgacatc ctcttcggtc tgaacgagac
      901 ccgctggggc caggtcgtgg acggcgtcta cgagaacctg tccctcgttg actggactcc
      961 gtcctgggcg gaagaagtcg gcgaatacaa gtatgacggc caggcctacg gcctcgtcat
     1021 ccagaacgtc ctcatgctct accgtaccga cgagctcggc accaacggcg aagagctgca
     1081 ctttgcgcac tgggccgacg tcgttgacag cggctacacc tggtaccgcc agaacaaggt
     1141 cggcggcacc accaacgcca acatcaactc cgccatgctc taccccttca ccgatcccgc
     1201 ttctcccgcc ggcggcatct ccgtcgatgg ctggaagacc ctgtggaagt actgcgccga
     1261 gggcaagttc ggcggcgacg actggaagta cggctttgat cctctgaaca agggcgacgt
     1321 tgccgtttcc tcgttctact cctcttctct ctacggcaag ctggacgccg cggcggaagg
     1381 ctccgagcac cccatcaccg actgctggaa gctcgttgac atcgacgacg gcacctacta
     1441 catcgccgag tacatcggca tcctgaacaa ggaaggccgc accgaggaag agaccgaagc
     1501 cgtcaaggcg ttcgccgagt ggttcggctc tgccgagacc caggctgcct gggccgaaga
     1561 gtttgactcc ttcccctgca accagggtgc tgttgagctg gtctacggct ccgacgtccc
     1621 cgagatctac ctcatcaaga acttcgccct cacgaccgtg gaaggcaccg acatgaccta
     1681 cgccgcttac gtcgctgccc acagctccga gtggacgaac atcatgacca acctcggctt
     1741 ctactgggcc gatgccagca acgctcctgc cgagcccgat tgggacaatc tcgactgggc
     1801 gaccctgacg cagaaggctg agtaattctc cgtcaccttg aaatcctgta tggcgagccc
     1861 ccgcggctcg ccatacttcg tattttccga aaatatccgc caaccaccca cagtccctgc
     1921 aaaagagagg aaggaatgcc actgtgatcc ggctcaataa catcgtcgtc aaattcggcg
     1981 acttcaccgc gctgcacgac atcaacgtcc aggtaaaaga gggcgaattt ttcacgttcc
     2041 tcggcccgtc cggctgcggc aaaacgacaa cgctccgcac cattaccggc ttcatcgagc
     2101 cggcggaggg ccaggtgttc gtccgcgata aggacatcac ccatgtgccc atcgaggacc
     2161 ggaatatcgg catcgtgttc cagagctacg cgctcttccc gacgatgacc gtgtacgaca
     2221 acatcgcgtt cggtctgcgc gtcaagaaaa tgaagaaggc cgaggtggac gaaaaagtcc
     2281 gcgctatcgc caaaaaggtc gatctttcgg acgagcagct caggaaagcc gtgtcgcagc
     2341 tctccggcgg ccagcagcag cgcgtcgcca tcgcccgcgc gctcgtcacc gagccggcga
     2401 tcatctgtct ggacgagccg ctctccaacc tggacgccaa gctccgcgtg cagctccgtc
     2461 tggagctcaa gaagatgcag aaggatttcg gcatcacgag catctacgtc acccacgatc
     2521 aggaggaggc ccttacgctt tccgaccgca tcgcggtgtt caacaagggc gagatcgagc
     2581 agatcggcac gccgaacgaa atttacaatc actccgccac ggaattcgtg tgcaacttca
     2641 tcggcgacat caaccgtctc tcggacgata tcgtggaaaa gctcaacgcc aaaggcgcgc
     2701 agcttgaccc gacacaccac cactacatcc gtctcgagcg tgtccatgtg aacgacgctc
     2761 agcacgcgga atccggcgcc gtttcgcttt ccggcaccgt ggaatcgaga gagtattacg
     2821 gtctgtatat aaagtattac atcaacgtcg gcagccagac gctcaaggtc atcgagcgca
     2881 acgacggcgt gcatctgcac gataccggcg acgccgtgac cgtcaccgtc caccccgacg
     2941 atctcatgag ctacgctccc aacggggagg aggccgagaa atgacactgc ggccgaagcc
     3001 cttccagccg atcaaaatgc tgcagatcat cggcggcgtt ttcttcgcgt atctcttctt
     3061 cgggtttatg ctgctgccgt gcctgaacac gctcaccagc attttcaccg tcaagaacgc
     3121 cgccggggaa accgatcccc tcgcggtcat ccgtttcttc ctcgccgggt ccatgtccca
     3181 gtatgtgtgg aactcgctca agctggcgct gtgccttgtc atcacggtca acgtcgtcgg
     3241 catttccatc gtgctgctga ccgagtactt tgacatcaag ggcgcaaaga tcctccgtct
     3301 cggctatatg acgacgctca tctactccgg cgtcgcgctc gtcacgggct acatgttcct
     3361 gtacgccagc gacggcatca tcaccacgca gctcaaggac gcgttcccga acatcaatgt
     3421 caactggttc accggcttca acgcggtgct cttcacgatg acgttcgcct gcacgagcaa
     3481 ccacgcgctc ttcctgcgca acgccatccg cggcatcgac tacaacaccg tggaggccgc
     3541 gcgcannnnn nnnnncccgc aacatgggcg cgaagccctt caaggtgctc tggaaggtcg
     3601 tattcccgac gcttctgccg acgctcttct cgctcacggt catgacgttt atcacgggcc
     3661 tgtgcgccat gagcgcgccg acgctgctcg gctatgactc catcaacccc gagatcgtcc
     3721 gtctggccgg ttcgtccgtg gcggacgagg cgttcccgca ggcgcgcgcg gcgctgcttt
     3781 caatcatcct cgcgtgcttc acgatcctcc tgctcaccgt tctctcgtcc tatgagcgca
     3841 aggggcatta cctctctgtt tccaagacga aggcgaagct ccagaagcag aagatcacga
     3901 accccgtggc gaacgtgctc gcgcatatct acgcctacgt cctgttcatc atctacatga
     3961 cgccggtcgt gatgatcgtc atcttctcgt tccagacgtt cagcgccgtg cgtatgcgca
     4021 agcttgatct ttcgcagtgg acgctcgtga actacttcgg caagcaggac tattcgtatc
     4081 tcacgtcccg cggcacctac aagatccgcg agggctcgat ctccggcgtg ttctccaacg
     4141 aggcgacgct cggcggcatc cagatgagct ttgtcatgtc cgtcatcgcc gcggcgctcg
     4201 cgtgcgtgat cgtcgttgtg gcctgcaact acatcttcaa gcacaagaag accggcacga
     4261 tcctggagta cgcgctgctc ttcccgtggc tgctgccgac gatcctcatc tgctattctt
     4321 accgcacgtt cttcaacagc ggcgacgtct ggtatgtgtt cggccagaac ctgtactatg
     4381 ccgaccgcgt gaggatattg atcgttatcg cgtatacggt agtaaagctt ccgttctcgc
     4441 tgcgcatgat caaagcgtcg ttctacgcga tcgacgagga gctggaggac gccgcgcgga
     4501 atctcggctc cgggccggtg cggacgttcc tcaaggtaaa gctcccgatc atcctgccga
     4561 gcgtgcttgc ggtgttcgcg ctgaacttca acgcgctctt taccgagtac gatatgtcgg
     4621 ccacatttgc gagtacctac ggcacaacgt acgccatggt catccagagc atgtgccgcg
     4681 aggagggcct gtacggctac aatgtcaacg cctctggccg ccggtgcgcc tccacggtgt
     4741 tcatcatgct cgtgtcgggc ctcattctgt atctcgtata cggcgtgggc gcgcgcgatc
     4801 ttggcgagcg gctcgaagcg cgtgaccgcc gccggaaacg catggatcgg ataaaagccg
     4861 tattccgcaa aaaacagccg gcggaggcgg caagccccca atgatcaaaa acatcctctt
     4921 tgatatgggc aacgtgctca tccggttcga gccgaagcag tatgtgaaaa acgccggtct
     4981 gtccgagagc gaccagcagc tcctgctgcg tgagctttac ggcagcatcg actgggtgcg
     5041 gctcgaccgc ggcacgctta ccgaagcgga agtcatcgac cggtgtgttt cgcgtgtcgg
     5101 cgagcatctc cgcggcgcgg tcgaaaagct cgtcggccac tgggaccggc cggagctgcc
     5161 cgtcgagggt atgaaagcgc tcacggacga gctttatgaa aaggggtacg ggctgtacct
     5221 tcttacgaac gcgggcccgc gccagcggga atactggccg cggtttcagg cgggcgagca
     5281 cttcccggag gagcgcgtgt accgctcggc ggacgtgcat ctcctcaagc ccgatccggc
     5341 gttctttgaa ggcgcgcttg aaaagttcgg tctggaccgc acggagtgcg tgttcattga
     5401 cgactcggcc gcgaatgccg agggcgcgca gcgtgtcggg ctcgacgcca tcgtcttctt
     5461 cggcgacgcc gcccgcctgc gcagagaatt gcgcgagcgc ggcgtggatg taagcgaata
     5521 aaagagagat gcgggagctg cggctcccgc atctttctat aattgacgga gcggccggca
     5581 aaagcccctc cgtcagcctt gcggctgccg gccccctgtt agggggccaa gtcgatttgc
     5641 ggaaaacggt cttgcctccc caaacatggg aggcggcaaa accgaaggtt ttgacggaga
     5701 ggtttatccc atcccctcgc ggagtggatt atagttagaa taacccgacg gcaggagggc
     5761 ttaaataaaa tatcgcataa ttcggtttat tctcaatatt cgattcgtca aaacgccaaa
     5821 aatccggcaa aaatctttgc cggattttta ctttgcctta tagccgctgc atgtcatatt
     5881 ctgttattat agaataacga gagagaggaa actttatgaa tcgctacgcg gtcgccgccg
     5941 tgctgtccgg cgcatttttc gggctgaccg gtctctttac ccgcacgctg accggcgcgg
     6001 gactgagcac gatgggcatt ctttccatcc gctgttccgt ggccgcgctc tgcttcttct
     6061 gcaccgctct cggcgatatc cgccagctca aaatgcacaa gaaggacttc tggctcttcg
     6121 ccgctgtcgg cattctcggg cagggcatgt tctcgttctg ctattataac gcgatcaaca
     6181 tgatgtccat ctccacggcg tgcatcctca tgtatctctc gccggtgttt gtcaccatca
     6241 tggcgcactt tgtgttcaag gacggcattt cccgccgcac ggtgttggcg atcattctct
     6301 gcatcgccgg gtgcgcgtgt gtgtccggct tcggcggcac ggtgacgctg ctcggtctca
     6361 tctgcgggct ggggtccgcg atcgtgttcg cgctcatcaa tattctcacg cgcgctctcc
     6421 ttggccgcgg ctataccggc aaggctgtca acttctggat ctgcgcgttt gccgctctgt
     6481 tcggcatcgt tatggatctg ctgctcttcc gtgaagggtt ttcccgcccg tttgcggtgc
     6541 tcttctccgg ctggaagatc ttccttgtgg gcatcaccat ggcgctcacg acgggctttc
     6601 tggcctaccg gttcttctcg ctggcgctgc acggctgcaa gagcggcacg gtgtccattc
     6661 tggcgagcag cgagcccgtg gcggaaacgc tcgtgagcgt attcgtgttc cgcgagccgt
     6721 tcggcgcgct ctgcgttgtc ggcatcgtgc tcgtgctcgc gggcatcgtt ttgcagaata
     6781 ccgcatccaa aacagaagcc tccgcgcagt aatttatccg tttcccgaaa gaaggggtcg
     6841 ttatgagttc ccggttttat gacatgtcct atccgcagta cacggtgcac gacgtttcgg
     6901 cgctgaccgg tctcacgccg cagaccatcc ggtactacga cagcattggc gtcgttgtgc
     6961 cgcagcgcgg cgcgcagggc cagcggctgt attcgtatta cgatgtgctc aatcttgtcc
     7021 ggcgcagtct ttataaggcg cagggcttct ccctgcagga aaccgagcag gtgctctccg
     7081 gcgcgatcac gccgcggctc ggcgcgctgc tggaggaaaa acgcgccgat atcgaggaaa
     7141 agcagcgcgc gctccggctc gcgcagttca atcttgaccg tctgtccgcg cagctcgacc
     7201 ggctcggcgt ctgccgcggg cgggtgatgt atctcgagcg cccggcgtgc tggcatgtac
     7261 cctacagccg cgacgggcac atctgctttg aagaggcgag ccgcgcagcg cactccagtt
     7321 gcggcagcgc gttcgcgttt tcgttctcgc tgcccgccgt gggcgagacg gacgactgcg
     7381 tggactggga cgtgacgatg ccctcgccgt ttgccgagga gctggggttc acggatctgc
     7441 cgggcgcggt gtttgtcccg gcgcggtact gcctgtacac cgtggtcgaa acgccgggta
     7501 tggacttcct tcgccgcgag gcgctccggc cgctgtacga ttttgccgcg cagcaaaatc
     7561 tcacgccgga ggggacggtc tatggccgcg acatcgtaaa cgccgcttcc gacggcgcgg
     7621 tgcttcgcta ttatgaagtg tggctgcccg tccgtgaaac gcagggctaa agccgctata
     7681 acgctgcgat taccggaaag cctgcgtttt ccggcgatcg cggcggtttt tgcttttcgg
     7741 atgccgtctt ttcgcacaaa aagccccccg aaaatcggga gaaaaaacta tttgcctgtc
     7801 cagcggcaaa atggtttatg ataaaaccat cggaaagcan nnnnnnnnnc cggagcgtta
     7861 tgaactccgg cgccggtttt tatatgcagg gaaggagctg tcagcgcttg cgcagagcga
     7921 gcggcgagcg cttccacttc tgcgggcggg gaagatgctc gagctgcgcg cggagaggtt
     7981 ccggtgcata ttgcagcacg agatcaaaga gctccgggtc atactgaaag aagaccatgg
     8041 ttttgacata acggttcgta ttttgaatga atcgccgccg ttcgttgacg gacaagtttc
     8101 ggctgctcag gtagacccaa taagtatcta cggcatttcc gacagaaccg acatccacgt
     8161 ccgccatacc gcaacaggga aggtcatccc aggtatagca atactttttc cggccgacat
     8221 acattatgca cccgctgcga tcaacagaaa tttttccgcc ggagaacgta aaaaccaata
     8281 ccttgagcat ccagattgtg ccgaaagcat gaaccccaat aataatgtaa tcgctaaagg
     8341 acggcccgtc ttcatttata atttttgtat ataagaaagt taaaaataga aagataacga
     8401 acaatgcgac aagcaggttg aannnnnnnn nnaaatagaa ttttgtaata ggaaattttt
     8461 tcattggcac ctcgccttcg acagtgctgt cgcaccggca tctcaatcaa tggggcgtga
     8521 aggtttctgc atcggtcggg gtgttccgga ggatatgcag tccttgcgga agcggaaaaa
     8581 cgaaagcaga aaatgcacac acaaaatcgc actgccaagc agatacggaa gaaaggccgg
     8641 tgttccgaaa ggatcggatg caaggccaag gaggaaaaag gccgcggagc ccacagcgtt
     8701 gcggagaagc cttttgagca gacgataatt gcgatattgg taataatacg ctgcgagcgg
     8761 ggcctgacag tcgacagctt tgccgtctac cgccagcacg aggctcggac ccgtaaaaga
     8821 cattccctca tacggaatcc gttttcccat tttccgctca aactcataaa acacccaact
     8881 gtcgctgttg gaaccgattt ccggagacat tagaaaatac cgaacatctc cgggctcgcc
     8941 ctgctcaaac cagtagttgg ttccagacaa ctgccgcagg aaaaacccct tttgtgccat
     9001 atcgtgcagc cagttttccg ttcggatggt ctgcgccagc gtcgatctgc ggcggacgga
     9061 tatcatcggt ttctgcataa ttctccctcc gctttttgaa acatgattat tggccaatcc
     9121 ttcgggcacg gcgaagtacc tcccttggtg attccggaag gacaatcaaa ggcgctcatt
     9181 cagccggaca acagaaaaaa gctccggctg cttcagtttg gggagctgat cgcggaagag
     9241 ctgcagcaat ccgtattcgg tatttatgta aaagcagcaa ttgtgatcgc tgaattctcc
     9301 gccgtcatcc agctccatgt atacctctgc gagaacatgt cctagccggt cttttcgcag
     9361 aaagcgcagg gacatacagg cggtggagtt gtcgcccctt tccttgttcg cccaacagca
     9421 ttcttcgacc tcgccgctca aaaatcgcgt tatttggcgg aacagctcgt cgatcaggga
     9481 atccgtcatg tatacggctg ttttcgcggt gatgaccggc gaagaacctg taatttcaaa
     9541 ttcggcgaaa tccgtgtcct gccatatctt ctgtatgctc aaataatcgt ccatcaggat
     9601 ttccccccct atggtttcaa aatgctccat caaagcccct catccgccgc cggaggaatt
     9661 cttcctatct tcctccgaaa aaaggctcca tcgtacgatg gagccttttt gattcagcag
     9721 agcacgctgc cggtggggat ggaatcatca agcatgacga gatgcagctc ttccttgccg
     9781 ttcaccatac gcacggcgga gagcagcata ccgcaggact cgagccccat catcttgcgc
     9841 ggcggcaggt tgacgatcgc ggcgaccgtc ttgccgacga gctcttccgg cttatagtgc
     9901 atggcgatgc cggagaggat ctggcgcgtt gtgcccgagc cgtcgtccag ctcaaagcgc
     9961 agcagcttct gcgacttttt caccgcctcg cacttgagca cacggcagac gcggaagtcg
    10021 cacttttcaa aatcgtcgaa caccacagtc ggcttgaacg gctcgatggg atccttcttc
    10081 ggcgccttga gcgcttcgag cgcggcgagc tccttttcca tgtcgatgcg cgggaagagg
    10141 ttttcgccct tcgtgacgga caccgcggcg gggagaacgc catacttctc cgcggcttcc
    10201 cagctcatgg cggtctcatc cgcgccgatc tgcgcggcga gcttctccat gctctcgggc
    10261 atgaagggct tgaggagcgt gccgcacacg cgcagcgttt cgagcagatt gtagagcacg
    10321 cgcgcgagac gcggcttctt tgcttcgtcc ttcgcaagca cccagggggc ggtctcgtcg
    10381 atgtacttgt tggcgcgggc gatcacccgg aacacctccg cgagagcgtt ctgcatctgg
    10441 aatttctcca tctgcgcggc gtacttgccg cgaagctcgc cgagcatcca gagaagctcc
    10501 gcgtcctcga cgccttcctc ctgcgtctcc ggcagcgtgc cgccgaagta tttgccggcc
    10561 atggccgtcg tgcgggagag aaggttgccg agatcgtttg caagatcggt attgatggtg
    10621 gagatcaaaa gttcgttgga gaagttgccg tccgagccga aggggaaggt gcgcagcagg
    10681 aagaagcgc
//
LOCUS       DAWAAE010000065        10518 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2851_length_10518_cov_3.420529, whole genome shotgun sequence.
ACCESSION   DAWAAE010000065 DAWAAE010000000
VERSION     DAWAAE010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_10518_cov_3.420529"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1722 a         3213 c         3290 g         2293 t
ORIGIN      
        1 gcccttgccg atgatcacac cgggacggga gcagtggatg aagatgcgga cctgggcgct
       61 gtcgcgctcg atctcaatgg tgggcacgcc ggcggcgtac agtctcttct tgaggtactt
      121 gcggatcttg tcgtcctcaa cgatcagatc gccgaccttg tcgggacggg cgtaccaacg
      181 ggaatcccag tccttgatga cgccgacgcg gaggccgtgc ggattaactt tctgtcccat
      241 aaatcaagcc tccttttccg ccacaacaac ggtgatgtgg ctggttctct tcaggatacg
      301 cgcgtagcgg ccacgggcgc tggcgcggcc gcgcttcatg gtcgggccgc cggtgaccat
      361 aaccttggac acatacagct tggagggatc catctcgtgg ttgttctcgg cgttcgcgca
      421 ggcgctgcac aggaccttgt acagcagggc ggcgccggag ttcggggtgt tctgcaggat
      481 gcccttggca agctcgacgt ccttgtcgcg gatcagatcg cagacgatct tgaccttgcg
      541 gggagaaata cgcgcgaatt tgtgttcagc tcttgcttcc atcagtatct ctcctcctta
      601 ctttccggca gtcttgctgc cggcgtggcc gcggaacgtg cgggtaggca cgaactcgcc
      661 cagcttgtga ccgaccatgt cctcggtgac gtacacgctg acgtgacggc ggccgtcatg
      721 cacagcgaag gtgtgaccga tgaaggacgg gaagatcgta gagctgcggg accaggtctt
      781 gaggaccttt ttctcgccgg ctttgttcag ttcatcgact ctcttgagca gctcaggagc
      841 tgcgaagggg cctttcttaa tgcttctgct catatttcct tcttccctcc ttacttagcg
      901 ttgcggcgct tgacaatcat cttgtcggtc gggttcttct tcttgcgggt cttgtaaccc
      961 agagtcggct tgccccacgg ggtgacaggg ccgggacggc cgaccgggga cttgccttcg
     1021 ccgccgccgt gcgggtggtc gcacgggttc atgaccgagc cgcggacttt cggacgccag
     1081 cccatgtgac ggacacggcc ggctttgccg agagagacgt tggcgtggtc gatgttgccg
     1141 acctggccga tggtggcgta gcagttcatg cggatcttgc gcatttcgcc ggacggcata
     1201 cggacggtcg cgagagcgcc ctccttcgcc atgagctgcg cggcgttgcc cgcggagcgg
     1261 acgagctgcg cacccttgcc cgggtacatt tcgatgttgt ggataacggt acccacgggg
     1321 atgttcgcca gaggcagagc gttgcccggc ttgatgtcgg cgccctcgcc ggcaacgatc
     1381 acatcaccgg cggcgagacc gaccggagcg aggatgtagc ggcgctcgct gtcgggatac
     1441 tcgaccagcg cgatgtaggc gctgcggttg gggtcgtact ccaggcggag gacctgcgcc
     1501 tcgacgttgc gcttgtcgcg cttaaagtcg atgatgcggt acttctgccg gttgccgccg
     1561 ccgtgatgac ggacggtgat gcggccgtag ttgttgcggc cggcgttctt cttttgacct
     1621 cgaccaggga cttctccggg gcggcatgct tgtcgacccc atcaaagccg gaaacggtca
     1681 tgtgccggcg ggcgggggtg ataggacgat aggttttaat agacattctc tttccctccc
     1741 ttaggccatg ccttcgaaga tctcaatgtt cttggaatct tcgctcagct tgacaacggc
     1801 tttcttccag gtcttcgcaa agccggcggg gtaacggccg gtgcgcttct gcttgccgtg
     1861 gacggtcgtg gtggtcacct tgacgacggt gacgccgaag atctcttcca cagccttctt
     1921 cacttcgatc ttggtggcgt ccttcgccac ttcaaaagcg aacttcttga tgtccaggtc
     1981 ttccatggac tgctcggtga tgaccgggcg gatgatcaca tcataaacgc tcttcatcag
     2041 cagtacacct cctcgatctt ggcaagggca gccttgtcga ccacgagaga gcgggccttc
     2101 ataatgtcgt aggtggagag aacggtggcg atcgtcgtct caacgccggg gatgttgcgg
     2161 gcggacttga tgacgttctc gttcacggtc tcggtgacga tcatggcgtt ctcggcgccg
     2221 agcttctcga gcagagaaac gcaggccttc gtcttgatct cgtcgaagct gagaccgtcc
     2281 accacgaaga tcttgctctc ggccgccttg acggacagag cgctcttcag ggcgatacgc
     2341 ttttccttgc ggttcatggt gtagcggtag ctacgcggct tcggggcaaa ggcgacgccg
     2401 ccgtgcgtcc acacagggct gcccttgtaa ccggcgcggg cgcggccggt gcccttctgg
     2461 cgccagggct tggcggtggt gtaggacacc tcgcccttgg tcagagcgct ctgcgtgccc
     2521 tgacggcagt tggccaggtg gttcttgatc gccgcatgaa ggacgatctc gttgggctcg
     2581 atgccgaaaa cggcttcgga gagctcaacc tcgccgacct gctcgccggc catgttgtaa
     2641 agattcgctt taggcataaa tcagactctc ctctctttag cggttgcggg cggaagcctt
     2701 ctgcgggttg gtacgcgcgg aagccttctg cgggttgaca ctcgtgctga cgctggcctg
     2761 cttgacgatg agcgtcttgg cggtgttctt gatgaacacg atgccgccct tcgggccggg
     2821 gatggcgccg cggatgacga tcatgttcag ttcgggatcg accttgacga cgtcaaggtt
     2881 ctgcaccgtg acctgctcgg cgcccatgtg accggcgccg atcttgccgg gaaggatgcg
     2941 gctgggatcc gtggaggagc ccatggagcc ggcatgacgg tggaccggac cgccgccgtg
     3001 ggtcatcggg gtgcgctggg cgccccagcg cttgatgacg ccggcgtagc ccttgccctt
     3061 gctgatgccg gtgacgtcga ccttgtcgcc ctctttgaag gcgtcagcct tgacaacgtc
     3121 gccgacgttc atctcgttgg cattctcgag gcggaactcg tgcagcacgc gcagcgcgcc
     3181 gacgccggcc ttgtcgagat gacccttttc gggtttgttg agcttgcgct cggggatttc
     3241 ctcgaagccg agctggacgg aagcatagcc ttccttttcc gcggacttct tctggaccac
     3301 tttgcacggg ccggcttcga taacggtcac ggggacgacc ttgccggtct cgtcgaagat
     3361 ctgcgtcatg ccgaccttct tgccgatgat ggcctttttc attggattgc tcctttcagg
     3421 ttattggtca ggtcgcatgg atcatatcgt ccggaggctc ctgacttgac cctgccttcg
     3481 taaggcgcgg ggttcaatac agattttgaa cgcgggggta atgccgggga ataaatataa
     3541 ggtgtatctc agagcttgat ctcgatctcg acaccggcgg gaagctccag ggacatgagc
     3601 gcttccaccg tcttctgggt gggggcgatg atgtcgatca ggcgcttgtg ggtgcggcgc
     3661 tcgaactgct cacggctgtc cttatacttg tgaacggcgc gaaggatcgt aatgacctct
     3721 ttctgcgtcg gcagggggat ggggccggaa accttcgcgt cggtacgctt ggcggtctcg
     3781 acgatggtct cggcggcctt gtcgatcagc tgatgatcgt aggccttgag gcggatgcgg
     3841 atcattttct ttgctgccat gtttgttcct ccaaaacttt cgtacttagg tggtggctgc
     3901 cacccagtgg tacggtgaga agttttcatc caccgcgccg tgcgtatcag acccgaaccg
     3961 aaaatagaac cggctttctg atggccggtt tttcttcccg ttccggcgat atacgcctct
     4021 tgctggccgg ggaaacgata aactctcagc cgcccgattt acggacatac tccacggaag
     4081 ttaccaccgt caaacgatgc aatctcccgc ttcaccgcat aatacgccct gcttcttcgc
     4141 acaggcgctt tgatagaata acaaaatccc ctcttaatgt caagagggga tagagaaaaa
     4201 ttcaaagttt ttttggggat ttctccgaat tttccgaata cggaaaattc ggctcagccg
     4261 ccgtcggtgc ggacgctgaa atgcatgaaa tcgcggcttg actgccacgt tccgccccag
     4321 ccccagccgt aggcggcgaa tgccctcaca acgctcccac ccgcggcgat cgagtacggg
     4381 ctcggctcgg cgagcgtacc ggcccactcg ctcttttcgg tgccgagcgg ccaccagccc
     4441 tgccctaccg tgatcttcac cgcgccgtcc acgcggtccg ccgcgcagtt cgcgtcggga
     4501 ttgatatcga ccgccgcgcc ccaggcgtgg cgcatggtat ccgtgcagcg cagtccgccg
     4561 acggaggcga tggggaattt ctcctcgtcc tcaaagatgc gctggaaaat ttcgtacaca
     4621 tccgcggcga ggtatttatg taccgtgagc ttgagcgcag cctgcgtttt cgtcccgtcc
     4681 ggctggagct gccatacgtt cacggtgaca tcgcgcatat acggcgcggc ttcctcggcg
     4741 ctcgcaaagt acgttttgtt ctcgtcgccg aagaagagct tgttaaaagc gccgcgcggc
     4801 tcgttgttgt tgagcatata accgttctcg cgcgcccaga tcataaacgg ctgcaggccg
     4861 ttcggcgcgt cggcaagacg gtaggggttg ttgtcggata gatcgaccgg ggcgcggcgg
     4921 gccggatcga gcaggcgcac catgacggcg ctcgcctgcg cgcgggtgag cttccgctcg
     4981 ccgcagaagg agccgtcctc cgctccggcg gtgtttttca tgccgttgat gatgcccttg
     5041 gcgtacacct gcgccaccgc gccgcggtac gcctccggaa tatacgccca gtccgggatc
     5101 ctgcgcgccg cgccgttcgt cacggcctcg gtctccccgc gcaccttggc gaggaagttt
     5161 cggatcatca cggccatttc gtagcgcgtg gtctttgcct gcagagcgtc gaacgtgcag
     5221 gggatgtcca gcccctcgag cacgccgttt tcggaaagca tcgcccagta cgcctccgcc
     5281 cagtgcttgc cgggcagctc gcttttgtac gtcagcgatg cgctcgtggc gagcattttg
     5341 atgaactcgc tccggcgcag atagcccgtc gggcggaacg tgccgtcctc aaagccgttg
     5401 acgatgccct tttcggtgca gaaatcgacg tactccgtgt accattcatc cgccggaacg
     5461 tccgggaacg cggcaaacgc cggagcggtg agcgccggca cgagcaggca cgcgcacaga
     5521 agcgccgcaa gaattctctt tctcatatct ggtcctccgc ggtgttcttt ttcgatgcga
     5581 tcagaagtcg aagaaaaacg gcatcatctc cgagagcttg tggctcacga agctcccgtc
     5641 gcccttggcg cagagcacgg tcatatgctt ggaaaactcc tgcagaaact ggcggcacga
     5701 gccgcaggga taacaatatt ctttgctgtt gccccagatg gcgatgcgga caaagtcgcg
     5761 gtggccgctg ctcacggctt tcaccgccgc ggtgcgctcg gcgcagatcg tgtcgccgta
     5821 ggcggcgttc tccacgttgc agccggtgaa gatcgtgccg tcgctgcact cgagcgccgc
     5881 gcccacgggg aatttcgagt acggcacata cgcatattcg gcggccttct tcgccatgtc
     5941 catcagttcc tgatcggtca tatccgtttg ctcctttttc cgccggcggt cagccgatat
     6001 tgactgtcca ggcggtaata ttattgaata cgtcgtactg gttgggcgac atgccggaga
     6061 tcacgccgag atgggagata acctcgcgcg tctcaaagca caccggcaga atcaccgtgg
     6121 tcgtcgtgag gagctgcagc cagtcgttta cggcctgcgc gcggtcgtcg tcctttgcgc
     6181 cgagataggc ctcggtgagc tcctccagct cgctgttttc ccagccgccg tagttgaggg
     6241 tgttgtcatc ctcccagtcg ccggagatca gcgtgagaat gttccagtcg ccggtcagcg
     6301 cgacctcggc gagatacata tccataacga tctcgggctc ctcctgatcg aagtcgatgt
     6361 atccggagcg cagcgcggcg agatagtcct tccatgtaag ctcgcgcagg ttgacgccga
     6421 tgccgatggc ttccagatcc tcggcgatcc ggcgcgcctc ctgcaccttg gcggcgttgt
     6481 cggcgcagac ggcaaagttg agctcaatgt ccaccttcat gccggagagc gcaaattcga
     6541 gcatgccgtc cgcgtccaga tcgcggcagc cgccgcgctc gagctcggtg aggcagcgcg
     6601 agggatcgta ggaaaactgc gaggcgatcg aggtatcgta cagcgcgctg ttcgggtgga
     6661 tggggagcac cgtcgccacg gcgttgccgt cgagcgtgtc cgatacgatc ttgtcgcggt
     6721 tcacgatcca gttcatcgcg tagcggtagg cgtcgtactg gaagaagcgg ctctggctgt
     6781 tgaagccgat gaagtggaga ttcgtggtgg tgatggtgcg cttttcgttt ttgccgccgt
     6841 agcccatgtt gtaaatgccg gtcgggtcgt tcacgacgag atcgacgaga ccgctttcaa
     6901 actcggtgat gagctcctcc atgccgcggt aggcgcgcag ctgcacctcg tcgagcggga
     6961 gactctcgct gttttcatac ccgtcaaact tcacgagtgc cgtgcggtct tcattataca
     7021 tatacgggcc cgatccgacg gggatctcgt cgccgacgga gccggacttg atgaccggga
     7081 tgctcatgcg ctgcgggagc tgcgcgttgt ccgcgccttc cacggcgaga tacacctcgc
     7141 cgttgtacgc gctcgaatag acgcgcccga cctgctgctg cagaggcttg tgtctctcgc
     7201 tcgtgaagat gcaggacacg gaatacgcca cgtcctgcgc ggtgagcgtg gagccgtcgt
     7261 gcatctggat gccctccctc accttgagca cccacacgcc cgcactgtct tcgttcttgg
     7321 agtaatacca gtccgaaagg atgcgcgacg tcacgttgaa gttctcatcg acctcgaaaa
     7381 gccggtcgta cacgaggcag tccacgagct gattgagcgt gctctgcgtc ttgacggggt
     7441 tgaggctctc ctcgtagtcg agtgagagcg agaacacatc gtccgcggcg ccggcgaagg
     7501 tgagcgtctg gccggagacg gtcgcggcgc tctcgtccag cgcgggatcg ggcgttgcct
     7561 ccggcccggc ggcggtctcg ccgcacgcgg caagcgagaa gagcgtcgcc gcggcgagca
     7621 gcagagcaaa gatctttctg tatttcataa tgcgatacct caaagcatga taaccgacgc
     7681 catcgtgccg cggcggccct tcgtggcgcg gtgcgaccag tacacgtcct gcatgcacat
     7741 cgtgcagtcc ggcgacacat cgatgtgctc cggtgcaagg ccgagctgca gaagtctccg
     7801 gcggttcgtt tccttgagat cgacgaggaa ttttccctcg acgccctctt ccggcgggca
     7861 gaggccctcg gcatcgctcc cgagcagagc gcgcaccgcc tccggcactt ccgggccggt
     7921 ctcaaagcag cagcgcgata tggccgggcc gatcgcggcg cggatgttct ccgctctcgc
     7981 gcccatgtcc tccatgcggc ggacaaccga gcccatcata tcggcgaccg ttcccttcca
     8041 gccgctgtgc gcggcggcaa cgatgcccgc cttcgggtcg cggaacagca ccggcacgca
     8101 gtccgcggtc cacacgcaga gcggcacgtc cttccggtcg gtgatgagcc cgtcgcagtc
     8161 ctcgggcggg tgcggcagcg gcggcatagc gcgctcggct tccgtcacat agcgcagcac
     8221 ggtgccgtgc acctgccgtg tgaagcaaaa gccctcggcg ggaaactccg ccgccgtccg
     8281 gaagcgctgc cagagtgcgc gcacgcgctc ctgccggctc tcgctctccc actcgggatc
     8341 gggctttgag ctgaacggga aatccccgac gtctccgagc cgccgcgaga agaggtgctt
     8401 cgtgccgagc acgtccgacg tgacccactg gatattgtct ttttcgcaga gtgaaaatgc
     8461 catagctgat tcctttacgg gggatgctga gcagcttgcc ccacccccat atttttaaat
     8521 agtatcctta ctgattcttg ccgcagcagt ccttgtactt catcggcaga ccgttcgggc
     8581 ggagcttccc gcacgggcag gggtcgttcc ggccgggctt tttgaccttt ttgaccggct
     8641 gcttgcgcac ggaggcgtcg cccccggcgt tcgccacggc gtttttcgtc acgctcttgc
     8701 gctcaacgcg ctcttccttg cggacgcgga cggtatagat acggcgcacg acctcggagc
     8761 ggatgccgga gatcatcgcc tcgaacatat cgaagccttc ctttttataa gcatcgatgg
     8821 gcttgatgtt gccgtagccg cgcagaccga tgccgcggcg gaggttgtcc atcgcgtcga
     8881 tatggtccat ccagtactcg tctacgacgc gcagcatgac gacgcgctcc agctcgcgca
     8941 tgagcggcgt gccgtccggc atggggccga aggcctcctc gcgtctggcg tatacgtccg
     9001 cggcgatggc cgcgagcgtt tcgttgagct cttccaccgt catgccgtcg tggtaaacga
     9061 tctcgcccgg ccgtacaaac agacgggtga gcggcacgac gcgttcggcc agatcgtccg
     9121 cgttctccgg cgggatgttg ccgaccgtct cggcgatcgt gtcgcgcacc atgccctgga
     9181 tattctctcg gatatcctcg ccgtcgagca ccttgcggcg ctgctcataa ataaggttgc
     9241 gctgcacgtt catcacgtcg tcaaactcga gcgtgctctt tcgcgcctgg aagttgcggc
     9301 tctcgacggt cttctgcgcc tgctcgagcg cgccggtgag cattttgtgc tcgatgggcg
     9361 tgtcctcatc gacgccgagg ctctcaaaca cgctgatgag ccgctcggag ccgtaaagac
     9421 gcatgacgtc gtccgtcagc gccaggaaaa accggctctc gccggggtcg ccctgacggc
     9481 cgcttcggcc gcgcagctgg ttatcgatac ggcgcgactc gtgtcgctcg gtgccgagga
     9541 tgtacaatcc gcccgcggcg cgcacctttt cggcctccgc gcccgtcacg gccttgtgct
     9601 cggccatctt ttcgcggaag agcgcgcgcg cttcgaggat cgtttcgtcc tcggtctcgg
     9661 cgtagccggt cgcctcgctg atcgtgtcct cgtcgtatcc ggcgcggcgg agatcggact
     9721 tggccagata ctccgggttg ccgccgagca tgatgtccgt gccgcggccg gccatgttcg
     9781 tggcgatcgt cacggcgccg agcttgcccg cctgcgcgac gatctccgct tccttctcgt
     9841 ggtgcttggc gttgagcacg ctgtgcggca cgccctgttt gcggagcagg gacgagatgt
     9901 attcgctctt ctcgatggag atcgtgccga cgagcacggg ctggcccttg ccatagcacg
     9961 ccttgatctg ctcgatgatg gcgcggtcct tgcccgcctg attcttgtat acaacgtcat
    10021 tccggtcgat gcggataacg ggcttgttcg tcggaatctc gacgatgtcg agcgcgtaga
    10081 tcgactggaa ctcctcctgc tcggtgagcg cggtgccggt catgccggag agcttcccgt
    10141 acagacggaa atagttctgg aacgtgatcg tggcaagggt cttgttctcc ttctgcacgt
    10201 ccacgtgctc cttggcctcg atggcctgat gcaggccctc ggagtaccgg cggccgagca
    10261 tgagacggcc ggtgaactca tcgacgatga tgatctcgcc gtccttgacg acgtagtcga
    10321 tgtcgcgccg catgacgccg tgcgccttga gcgcctggtt gatgtggtgc gagagcgtgg
    10381 agttttccat gtcggcgaga ttttcgagat tgaacgccgc ctccgccttg gcaatgccgc
    10441 gggcggtgag cgtggcggtg cgcgcctttt catcgacgat gtagtcggcg tcgatatcgt
    10501 cagactcctc ttccttct
//
LOCUS       DAWAAE010000066        10446 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2874_length_10446_cov_3.384756, whole genome shotgun sequence.
ACCESSION   DAWAAE010000066 DAWAAE010000000
VERSION     DAWAAE010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_10446_cov_3.384756"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1451..1460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5951..5960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9011..9020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1979 a         3073 c         3256 g         2108 t
ORIGIN      
        1 aatttttaca agggtggagt ctatctgttt tcgataggct tttgcggcgc tgccgttgcc
       61 cttgtatttt tgcgcccatt tttacgttct tattatgatc tggaggtcta tttacccatg
      121 aaaagaataa ttcccctgtg ccttgcgttg gtgatgaccg ttggcctgct gacaggctgc
      181 ggcaagcaga atgagccggc ggcttcggat gaaacgagac tgcgcgttgt caccacgatt
      241 ttccccgaat acgattgggt cagagaaatt ctcggtgaca aggcggataa cgccgaagtg
      301 accatgctgc tggataatgg cgttgacctg cacagctatc agcccaccgc ggatgatatt
      361 gtcaaaatct ccgagtgcga cctgttcatc tatgttggcg gcgagtcgga tgactgggtg
      421 gacgatgctc tgaaaaatgc cgccaacaag aacaggaagg tcatcaacct gctggaagct
      481 cttggcgaca gcgttaaaac cgaagaagtg gtcgagggta tgcaggaaga ggaacacgac
      541 cacgaccatg accacgaaga taccgatgag catgacgatg caaaagaaca cgacaacgag
      601 gaagaagcgg agtacgacga gcatgtctgg ctctctctga aaaacgccca aacgctttgc
      661 agcgcgattt cctccgtgct gcagcagatc gaccccgaca ataaggatac ctatgctgcg
      721 aatgcttccg cttatatcaa aaagctgtcc gctcttgacg cggactatca ggctgccgtg
      781 aatgctgcgg cctgtaagac cgtcctgttc ggcgaccgct tcccgttccg ctatctggcg
      841 gaggattacg gcttgcgcta ctatgccgcc tttgcgggct gctccgccga gagtgaagcc
      901 agctttgaga ccgtttcgtt ccttgccgga aaggtggacg agctgaacct gccctgcgtg
      961 ctgaccattg agggcgtaca gcacaagatt gccgaaacca ttgtccggaa cacggctgcg
     1021 aaaaatcaaa aggtgctgac gatggattcc atgcagtcca ccacttccaa agatgcggca
     1081 aacggcacga cctatctctc cgttatggag cggaaccttt ccgtgctgaa ggaagcactg
     1141 ggttaaggag gcaggataat ggctcagctc atttgtcaag atctctgcgt cggctacgat
     1201 ggaaaggctg ttctgcaaga cctcagtttt gcggcctttt ccggcgatta cctctgtatc
     1261 gtaggagaaa acggctccgg caagagcaca cttatgaaaa cgatcctcgg cttgcagcag
     1321 cctgtccgcg gcaggatttt gacgctcgac gggctgagaa aaaatgagat cggttatctg
     1381 ccgcagcaaa cgcaggtgca aaaagacttc ccggcatccg tgagagagat cgttctctcc
     1441 gggtgtcagg nnnnnnnnnn ggtgtcaggg acgctgcggc agccgtccct tttacaacaa
     1501 agaagaaaag cagcttgcgg cggatgcgat ggaaaaaatg cagatcgcgc agcttgcaaa
     1561 gcggtgctac cgggagcttt ccggcgggca gcagcagcgg gtgctgcttg cccgtgcgct
     1621 gtgcgccaca cggaaaatgc tgcttctgga cgagcctgtc tccgggcttg atccaaaagt
     1681 tacggcggag atgtatgcgc ttatcgaaaa gctcaaccgg gaggacggca tcaccgtgat
     1741 tatgatctcc cacgacattg ccgcagcagt caaatacgcc agccacattc tgcatatcgg
     1801 cgacgccgtt ttcttcggaa caaagacgga gtatctccaa agcccacagg gacggctgtt
     1861 tgccgcggag aaaggcggcg acagacaatg accgctatta tggaaaaact ggccagctat
     1921 tgggcatacc cgtttgtccg gtatgcattt gttgtcggcg tgctgatcgc cctgtgctcg
     1981 tcgctgctgg gcgtgacgct ggtgctgaag cgcttttcct tcatcggcga cgggctttcc
     2041 catgtggcgt tcggcgcaat ggccatcgct gccgtgctgc agctcaccaa cgaaatgccg
     2101 ctggtgatgg ctgttacggt cctcagcgcc gtgctgcttc tgcgtaccgg acagaacacg
     2161 aagatcaagg gcgatgcagc catcgccatg atctcggtag gggcgctggc tgtcggatat
     2221 ctgctgatga acatcttttc cacctcgtcc aacctctcgg gcgatgtgtg cagtacgctg
     2281 ttcggctcta cctccatcct cactctgtct ctgacggagg tgtggctttg cgcagggatg
     2341 tcgatatcgg tggtggcggt gtttatcctg ttttacaaca aaatcttcgc tgtgaccttt
     2401 gatgagaact tcgcaagggc gaccggcacg aaagcaggac tttacaatct gctgatcgct
     2461 gttgttacgg ctgtcatcat tgtgcttgcg atgaatcttg tcggctcatt gctgatctcc
     2521 gcactggtga tttttcccgc gctgtccgct atgcgcgtat tcaagagctt ccgcggtgtg
     2581 accatctgcg cggcgattct gtcggtcgtg tgttcgctga caggtatttt ggtatcgatc
     2641 cttgccggta cgccggtagg ctcgaccatt gtcgcggttc agatcattgc tttcgctctg
     2701 tcctggctgg caggcacggt cgttggaggt gtgagaaaat gaaaaaacgg ctctgcgtat
     2761 tgctgatcgt cataacgctc tgctctcttg cggcctgcgg cgcggcggct gcgtctgcac
     2821 cggagcagac gacgcctgtg ctcccggcgg aaacgccgct gccgacaaga gagccgacgc
     2881 ctctcccaac ggcggagcct acaccgcagc cgctttcgga gacggagacc ggcgaactgg
     2941 atctgacagg catgagcggc accatgctct atacgatgat ctataatatg atgaagcagc
     3001 cggacgacta tcttggccgg acgatccgcg tcaaagggca gttttccgct tatgtggatg
     3061 agaagagcgg gaaaagctat tatgcctgct acattgccga cgctgccgga tgctgcgcgc
     3121 aggggctgga atttcttccg gccgacgcgc tttcctatcc ggacgatttt cccgagccgg
     3181 ggagggatat cagcgtgagg ggtgagcttg ccttgatgca ggaagagaac ggctttcgat
     3241 atcttgtgct gaaggatgcg tcctttaccg taacataaac ataaaatcac agatgcagac
     3301 gcaaagagcc ggtatcccgg aaaaggggat accggctctt tgccagcaat ggcttgaaat
     3361 ttctcgttaa gggtcgtttt tccaaaggac tagatccccc agaacgatct tctgaatacc
     3421 gtctatgttg atcgcgctgc cccattcgca atggagctcc accgcgccgc ttgtgtacgg
     3481 agcgctcagc gcgccgccgg tggagaaggc cgagccgtcg gagaagaaga gctgcgattc
     3541 gatgcatacc ttgtcctcca gcatggacca gacttccgct tccgccggat cgccgttcgg
     3601 cgaatggatg gcctcggctc cctcgtagcg caccttccac accgcgccga acggcgtcag
     3661 ctcgagcccg gaaatctcga tcgacttgcc cgacgccgcg tcggtgtaga cggcgtgtcc
     3721 aaagtcaaaa taccgggtct gctcgtccac cggcgtaaag gacaccggcc cgaaggtctg
     3781 catctcgata tccttgtcgg acatatgcac aagaagcggg aagctgcccg tgccgagcgc
     3841 cgctgcggcc ttttcgagga acatgtcctc cacgatgcac tcaagcgtca gcgtctgcgt
     3901 ctctctgtcg taggcgtcgt attgcagcac ggtgtcgcgg atcgcttcct gtccgtgcag
     3961 cgtgatgccg gacggggtgc ggggcgaggc atagccgccg agatcggtgc cgtcgatgct
     4021 ccaggaaaag ttcagcgctc ccggatatcc ggggtaatcg gcgatgcatt tctcgcttac
     4081 cggcgagata ttcacataga cgcgctgaaa ttctccggcg tttacggcgg agagcaggac
     4141 aagctcactt tccgatcctt cctcttccgg aacgtcggag accgtgctgt tggctgcctc
     4201 cgggacgggg tattcggcat agctgctcac gttgttttct tcgatcttca gatcggcctt
     4261 gatctcctcc tgccgggcgg cgtgcgtgct ccatgccgcg taagccgtga tccccagcgc
     4321 aaggatcagc gccgcggcga gcacgatgct gagaaaacgt ttctttttca tgcgtacgat
     4381 cctttccggg ccttcctgca tcgtcccggg agcatagacc gcggcttcac tgacaaaccg
     4441 gtcgtccaga ccgcccagcg cctgagtgat ggtttcccgt ttcatacaag caatccctcc
     4501 ctgataaggt agtgtcggag tttttcccgt aggcggtaaa ggcggacagc cacgctgttg
     4561 gcggtggagc cgatcatggc ggcgatatcc tgcatcgaat cggagtacca gtaccggcgc
     4621 acgaaaagga accggtcggt atagctcagc gacgagagaa atgcattgat cgcctcggag
     4681 agttccttcg catcgtacgc ctcctccacg ctgccgccgg cggcgagaca gccttccagc
     4741 tcatcgagcg cggcgtcgta gcggctgttt cgctttttcg cggtgttggc gtggtacttc
     4801 gccgcggcaa gattgcgcgc gatcttgcat acgaacgtcc gcagcggatc gggcttctgc
     4861 ggcgggatgg cattccaaac ggcgagatag gtgtcgttga ggcactcctc cgcgtccctt
     4921 tcgctgccga gaatgttccg ggccacgcgc gccgcggcgc tgccgtgcgc tttggcaagc
     4981 tctgcgatcg cctgctccga gcgctcaaaa aacaggcgga tgatctcctc atcggtcatg
     5041 ggctgtcacc tcctttcgcc ttcacccata tactatggat cgacacccag tcattaacag
     5101 aaaaaagaaa tatttaagca aatatgcgaa atgcgccccg agcggggcgc atttcatatt
     5161 atggattctt tagagcgtgc aggcatagac gaacgccacg agtgtgagca gcacgccgga
     5221 gacgatcgag ccggaccaga gaacataccg aagatcggcg ccctccagcg gcttgcgctt
     5281 cggtctcacc ggcgcggcgg ggcgctgcgg cgctgcggcg tacggcggcg cgggatagct
     5341 ctccggcgcg ggaatgccgc ttccgtaaaa ggcggtctca tattccggct gcggggcgcg
     5401 cggcatctcc ggctgcggcg gggcataggg tgcctccggc gcgggggcgg agccctgcgg
     5461 cgggtagggc ggatagacgg gctgcggcgc gtaatatgcg ttcgggggat aggcggcgtt
     5521 ataccgcgga tcgtacgccg gagcagcgta aggatcgtag tacggcgcgc cgtaggcggg
     5581 cgctgcatag ccctgcggcg gatagggcac ggcggcgtaa ggatcgtacc cggacggggg
     5641 atagggcgct gcatagccgc cgtaggggtc atagccctgc tgcggcgcgt agctctgcgg
     5701 tatatacgcc ggagcgctgc ggcgctcaaa cggcatatcg ccgccgaccg tttcgtatac
     5761 cggcacatcc cgcggcggtt tcttcggctt ttcgggcgcg gtgaacgggt tttcccattc
     5821 cggctgcgcc atggcctcgg gagcgtatgt acgcgcggcg gcctcgtcgc gggggatgcg
     5881 gtcctgcatc tcttccgcgg ggcgggcaaa gcgctgccgg atgctgccca tgacctccgc
     5941 cgccgtgggg nnnnnnnnnn gcttcggcgg ggtgaattcc tgctttcgca gcgcctgcat
     6001 catttccgcc tccgagccga aggcacggct ggggtcggca ctcgcggcgc gatccccgcc
     6061 ggaaaatttc tttcggatat cggcaaaatt catcggcgcg cctccacgat ctgccgggcg
     6121 cgctcgccgt cgtgggcgat ctgcgcgcca agctcgtcga gatcggcaaa acgccgctcc
     6181 ggacgcagaa aatcaaagaa ctcaagcgca agtgtgcggc catagagatt cccgtcaaac
     6241 ccgagcagat ggctctctac ggtgacgacc ggctcgctgc tcacggtggg gcggacgccg
     6301 atgttcgtaa cggcgggaaa ctcgccctcc ggcaaaaggg cgcgcgcggc ataaacgccg
     6361 tgcggcggaa cgagcacgcc gggcgcgaac tgcatgttga ccgtcggcgc gccgagatga
     6421 tggccgagcc ggtggccgtg gcggacgacg tccgtgagga aatgcggatg ccccatgaaa
     6481 tactcggcgc gaagcatgtc cccctcacgc agaagcgagc ggatgtaggt ggagctgacg
     6541 gtgatgccgt caatctcgac cttgtcgacc acatcgcatg taaggccgtt tgccgcgcag
     6601 taatcgcgca ggcgcccggt atttccctcg ccgcgccagc cgaagcggaa atcgtagccg
     6661 acgacaaagc cgacgacgcc gagctctgcc tgaatgctgc gcagaaactc ctcccaaggc
     6721 atatgcatcg tctcctccgt gaagcggaaa aactccacgc ggtcgatgcc gaacagccgt
     6781 cggatgagat tcgcgcggtc aggcgcgctg ttgatgagcg gcacgggcgc accctgcacg
     6841 agcgtgtcag ggtgcgtgtc gaaggtcata acggcacctt cgacgccgag cgcttcggcg
     6901 cgctcgatcg tgcggttcaa aagcgccgcg tggccgcggt gtacgccgtc aaaaaatcca
     6961 agagcgagaa cgcattttct ctctgccatg aatgatcaaa cctcaaaaaa actttttacc
     7021 gtaacggcct cgccgtttgc gacgcgcccg atggcgagga attcgccgtt ttcggaataa
     7081 aagcgcgtct ctccgtccga cgcgtcggtc cggaacggat ttccgcagcg gcagcgcttt
     7141 tcctgcgcgg cggtgagcgt cagagacgcc gccgcggaaa agagcgtgtc cgccgggaga
     7201 agcgtatcgc ggtcggcgcg cgtgagctcg ccgatgcgcc gggcattttc cacggaaaag
     7261 cccccggagc gcgtgcgccg cagcgccgcc atgcacccgc cgcagccgag atactcaccg
     7321 agatcgtggc agagcgtgcg gatgtaggtg cccttcgagc agcggatacg gagcatgtga
     7381 tcgccgtttt cctgcccgag atagtcgatg gaatgaatgg ttatgcgcct tgccttacgc
     7441 tccacctccc cgccgcggcg ggcgatgtcg tagagcttcc ggccgttaat tttaatggcg
     7501 ctgtacatcg gcgggatctg ttcgatctcg ccggtgaacc ggggaagagc ggcgcggaca
     7561 tcctcttccg agggtaagac gtccgacaag cgcagaaggt tgcctgtaat atcctgggta
     7621 tcggtaacga tgccgggccg gaacgcagcg atatattcct tcgtttccgc ctcggcaaac
     7681 gccgccgcgc gcgtggcgcg gccgacgagc acgacaagaa gccccgtagc cagcggatcg
     7741 agcgtaccgg cgtggccgat gcgacgctcg cccagaatgc cccggagctt ggaaacgcag
     7801 tcctggcttg tccagccctc gtccttatcg agaagcagaa taccgtttac gcccatactt
     7861 cctccacggc gcggcgcacc gcgtccgccg tttcgtccgg cgggagatgc agctcgcacc
     7921 cggacgccat tttgtgtccg ccgccgccga accgggcgca gacctgcgag gcatccacgc
     7981 cgccgtccgt gcgcagggat accttgctgc gcggcgggtc aacggagagc tcgcgcaccg
     8041 ttatggccac acggttgccc cggacctgtc cggcgagcga ggcgagatcg ctgcagtcgt
     8101 cctcggtaac accggcgcgg cgcagcatgt caagcgtaat gacggcaata ttgatcgcgt
     8161 gatcgcggta ggagcggagc gaggagcaca ccatgccctc gagcgcgagc cgcgccgggg
     8221 acttggtgcg gaaaaagatc ttgttgagca gcggcagatc cgccccggcg tcgcaaaggc
     8281 gcgcggcggc gcggaacgtg gcgctgttgg tgttcgcgta gcagaagcag cccgtgtcgg
     8341 tggagacggc gatatagaga aggtcggcct ccgttttgtc cggcccgccg accagcgcat
     8401 cgatgagcgc gagaacaagc tcgccgcagg cggcgctgtg cgtccagata acggcgttct
     8461 ccggctgctc gccgcggggg acatggtggt cgagcttgag ggagatcttc ccttcaaacc
     8521 cgagggcaag gagcttgttc tccgccacat ccacgctcac gcacgtctcg gggacgaacc
     8581 cctccggcac atagtacggc gaaacgaagg ggacgaacat ctccgtgatc tcggggtttt
     8641 tataaagatg cgccgttttt cccaaacggc gcagtgcgtg gcacagcgca gaggcgcttc
     8701 cgagagtgtc cccgtccggc ctctgatgcg tcaccagaag ataattgtcg tgcgcgcgaa
     8761 gatacgcggc gcattccaga agttccatgg ttacccgttt tccccgtctc tgggcgaagc
     8821 gccgtccaga tggcggatga tgtcgttgat gtgagcgccg tgctcgatgg agtgatcgag
     8881 ctcaaagatc agctccggcg tataccgcag cgagagcgag gtgcccagct cgtgccgcag
     8941 ccagccgctg cacgatttga gacccttttt gagctctttt tcgttcacgt cgccgagcac
     9001 gctcagatac nnnnnnnnnn ctcagataca cggtgcagta gcgcagatcg cccgtcgttt
     9061 cggtgcggac gatgctcagc agatcgccct gctgcacgcg cggatccttc acctcgcgca
     9121 ggagcgaggc gagtgtgcgc cggatatcgt cgttaatgcg ctggatgtga ttggacggca
     9181 taatttaccc ttcctttctt tacagctgct cgttgacgaa gcactcgatg acgtcgcctt
     9241 ccttgatgtc gttgaacttt tcgacctgca tgccgcattc gtaaccggcg gagacttcct
     9301 tgacgtcgtc cttgaagcgg cgcagcgaag caagctcgcc ctcgaaaatg acgatgttgt
     9361 cgcggttgac gcgcacgctg cagttgcgct ggatcttgcc gtcctgcaca tagcagccgc
     9421 acacggtgcc gaccttggac acgcggaacg tctggcggac ctcggcgtgg ccgatgatga
     9481 cttcgtggaa cttcggcgcg agcatgccct tcatggcggc ctcgacgtcg ttgatgcagt
     9541 cataaatgac gcggtacatg cgcagctcga ccttggagcg ctcggcgctg tcgcgcgcgg
     9601 cgttgtccgg acggacgttg aagccgacga tgacggcgtt gctcgtcgag gcgagcatga
     9661 cgtccgactc gttgatcgcg ccgacgccgg aatggatgac gcgcacgcgc acttcctcgt
     9721 tggagagctt ttcgagcgag gtcttgacgg cctcggcgct gccctgcacg tccgccttga
     9781 cgatgatgtt gaagtccttg agatcgccct cctggatgcg ggcgaacaga tcctcgagcg
     9841 agaccttctt gcccgcgccc gcggcagcgg cgctgcgctg cgcggcaagg cgctcctcgg
     9901 cgagagcgcg cgccatgcgc tcgtcggcga cggcgttgaa cacgtcgccc gcggacggca
     9961 cttcggacat accggcgatc tccaccggga cggaggggcc ggcatcggtg atgcgctcgc
    10021 cgcggtcgtt cgtcatcacg cgcacacggc cgacggcggt accggcaatg atgatgtcgc
    10081 cggtgtggag cgtgccgttc tgcacgagaa cggtcatgat cgggccgcgg cccttgtcga
    10141 gacgcgcttc cacgaccgtg ccgcgcgccg gacggttcgg gttggccttg agctccatga
    10201 tctcggcctg cagcacgaga ttttccagaa gctccttgac gcccgcgccg gtctttgcgg
    10261 agatcgggca gacgatcgta tcgccgcccc actcttcggg gacgagatcg tatttggtga
    10321 gatcctgctt gatgcggtcg gggttcgcgc cggggagatc catcttgttg atcgccacga
    10381 cgatcggcgt accggcggcc ttggcgtggt tgatggactc gatcgtctgc ggcatgatgc
    10441 cgtcat
//
LOCUS       DAWAAE010000067        10433 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2880_length_10433_cov_3.168433, whole genome shotgun sequence.
ACCESSION   DAWAAE010000067 DAWAAE010000000
VERSION     DAWAAE010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_10433_cov_3.168433"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    368..377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2973..2982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3708..3717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1938 a         3108 c         3233 g         2124 t
ORIGIN      
        1 ggtgatggag taggtcggga tcgggtactg gaagccgcgg ccgttggcgt cgccctcggt
       61 catgatctcg atgaacgcct tgttcaccat gtccatttcc ttctggcagt cgccgtaggt
      121 gaagtcctgc tccttgccgc cgacgatcgc gggcagatcg cgcaggtcgg ccggaacggt
      181 ccagtcgagc gtgatgttgg agaacggcgc ctgcgtgccc cagcgggacg gcgtgttcac
      241 gccgaagatg aacgactgga tgcactgctt gacttccttc tgggagagct tgtccacgcg
      301 cacgaacggc gcgagatacg tgtcgaacga ggagaacgcc tgcgcgcccg cccattcgtt
      361 ctgcatgnnn nnnnnnnctg ctgaatgagc tgcttcaggc tccagccggc gcagtagccg
      421 gtgagcatgc tcagatcgtg cagatgcaga tcgccgttgc ggtgggcgtt ggcgatctct
      481 tcgtcgtaca cctcgctcag ccagtagttg gcggtgatcg cgccggagtt cgagaggatg
      541 agaccgccga cggagtaggt gacggtggag ttttccttca cgcgccagtc gttgatcttg
      601 agatagttat caacggtctt cttatagtcg agcaccgtgt cgcttacggc gcggagattc
      661 tcgtgctgct tgcggtagag gatgtacgcc ttggcaacgt tctcgtagcc gccgcgggag
      721 aggacgctct caacgctgtc ctggatatcc tcgatgtgga tgagctcgtc ggtgatcttc
      781 ggctggaaat ccgccgtgac cataacggcc agaaaatcga tgacgctctg gttataattg
      841 gtttcggtgg cgtcgaacgc ctttttcatc gcgttggcga tcttgctgat ctcaaagtca
      901 acgatcctgc cgtcgcgctt cacaacccgg tacatagtct tcctccatat atttctataa
      961 ttttaatcaa agcaacgcgc gccgcatgtc ctgtccgccg cgcgccatta tcatatcatc
     1021 gagtcttgac ggtgtcaata tatagtgtct gattttcatt atttgtttct tttgccacta
     1081 aatatagtgc cgccgcccca aaaacggcgg ggcggcggca tatattgcgg agacggggat
     1141 cagtccatgc cgcggatctc ggcgttcggt atggcgggca gcagtatcgc ccggaacgac
     1201 tccatctcct cgcgcgtgaa ggcggcgcag ccgccggaga tcacgtcgcc ggaatccttg
     1261 aatgcctgaa ggaaatactt ctcgttcccc ggcagccacg cggcgatatc ggcaaaatcc
     1321 tccgccgagt gcaggccgcg cacgaccgtc gtgcggaatt caaaatcggt atcgccgtcc
     1381 atgagcatcc gggcgcactc ctccacggcg gccaggccgg gacgcagcgt gccggtaacg
     1441 gcggaatagt tctcccgtcc ggctttgatg tccatggcga catagtcggc gagcttttcg
     1501 ctcaaaatgg cgcggagcat ggcggggttc gtgccgttcg tatcgagctt cacggcaaaa
     1561 ccgagagctt tgatcttccg cagaaactcc ggcagatcgc gctgcagcgt cggctcgccg
     1621 ccggtgacgg caacgccgtc gagcagcccc ttccggtggg cgaggaacga gaagatctcc
     1681 tcctccggga tcaccggctg gctctcgggc tcgagcacga gcgaggcgtt gtggcagaag
     1741 gggcagcgcc acgggcagtg cgcggtgaac accgtgcagg cggtatgacc cggatagtcg
     1801 agcagcgtta atttctgcaa accgtgaatg accatatctt gccctccttt tgccgaacga
     1861 tacaatatca tgtatgagcc cggattgcaa ttggcaaata cggcgaaaaa acgcgccgcg
     1921 ccgcgcttga cagaaagcgc gcgccttttt atactcaaag agaacaaaac gggcgaaatg
     1981 gagaagaatt atgagtgcgt tttttctgac cggcgcggtg cagaccggca aaagcacggc
     2041 catccgccgc tttttctgtg caaattctgc gctccggcgg ggcggttttc tcaccgtgag
     2101 catcccgacg gggaccggat ttgacgtttt catcgtcccg cctgtgtgga cggagtgcga
     2161 tctcacgccg gacgccctcg tcgggcggcg cggcggcgct tacgaaaagc atccggagca
     2221 ttttgacggc cgcggctgtg cgctgctcgc ggagcgggaa tgcgacgtta tcctcatgga
     2281 cgagcttggg cgcatggagc tggacgcgca cgcattccgc gccgcggtgc tcgcggcgat
     2341 acattccggc acaccggtgc tcggcgtcat caagccggag cataatgcgt ttctggacga
     2401 ggtgcgcgcg cagagcggcg tggagctttt cccgcttacg atagaaaacc gcgaggaagc
     2461 ccctgcggcg atcgcggcgt ggtatgcgcg tgcttcgcgc gaaaagcggg cataatgcgg
     2521 caggggggat gagcttggag aaaaagtata cactgtggga cgacctctgc cggatcgtgc
     2581 agagcgtctt tcttgccgcg gagatcacgg ttctcgccga cctgctgttc gccgcggggg
     2641 agaatcctct gccgcgcgcg gcgttctggg gactgtttct tgccgcggcg gcggcgctct
     2701 cgctctggcg ggggttcacg cgaaaggggc ggcgcatcgt attcctttcc atagcgggcg
     2761 cggccgtcct ctcggcgctc gcgctcttcg ccgcgtggag cgccgcggcg ccgaaaacgg
     2821 cgtacgaagc gccggagacg gagccgaaag cgatcttttc cgaaaagcgc gtgctcgccg
     2881 tcgtgccgca cgaggacgac gatctcaatc tcctgagcgg agtgacgggg cagttcaccg
     2941 acgccggaag caaggtgtac gttgtgttcg tcnnnnnnnn nnacggcgtg ccggaggaga
     3001 atatcatctt tctcggctac ggcgacagca tcccggacga cgggatacac atttataatg
     3061 cagcaccaaa cgccgtgacg ccgtcgctga gcgggcggac ggaaacgcac gccgcgccga
     3121 accacgaagc gtaccgcgaa gggacgccgt atacgcgcga aaacctgctc ggcgatctgc
     3181 gcagcgtgat cgaggagatc cgcgccgacg tgatcttctg cgtggattat gatgagaaca
     3241 tcgaccaccg cgcggtgacg atgcttttcg atgaagcgct cggcgaaata ctgaccgctg
     3301 cgccggacta cgacccgctc gtgctcaagg gctttaccta ctccacggcg ttccacgccc
     3361 cggcggactt ttacgattcc gtgaatctgc tctccacggt aaaccctgac ggggagcgca
     3421 tggaaaacgg cgtggatcgt tgggacgcgc gcgtgcgcct gccgatggac ggccgcgctc
     3481 tttcgcgctc gatcacggaa tgccggagct ttgccgtttc aagagaatac gaaagccaga
     3541 tgctctggcg cattgcgccg cgcatcatca acggcgataa ggtgttctgg cagcggtgca
     3601 ccggctcgct tctgtacggc gcggcggtgc gcgtatcgtc cggcagcggc gcggagctta
     3661 cggacttccg gctgctccac tcggaggacc ttgccggaag ggagctgnnn nnnnnnngcg
     3721 ccgtgtggac gccggagagc ggtgacaccg cgcgcgaggc ggaattttcg tttctcgccg
     3781 cggacgtgac ggaggtgcgg ctctacgata acccctcgcc ggaggataac gtcctcgccg
     3841 cggagatcgt gttcccgagc gggaaccggt acgccgtcgg cgcgctcgac cccgccggta
     3901 cgttggtgcc ggtggacgag ccggactgcg aaggcttcac cgtccggctg ctggaaacgg
     3961 agggcgagca cgcggggctt acggaagcgg aggcgtattc cggcgagcac gatgtaatgc
     4021 cgccgcttat aaagctcaca gacggggacg gaaatttcat ctacgactac cggctctccc
     4081 gcggggagac ggaggcggtg ctctcgctct acgccctgag cgcgagcgat gatttgaccg
     4141 gctataccgt gacctgcgag ggcgagggct gcgccgcggc ggtccgggac ggcgcgcttt
     4201 ccgtgacctg cccgcgcgga aagagctgta ccgtcacggt gacggacgaa acgggtacgc
     4261 tttcggacag cgtatacgtt gcgcacgaga ccgcttccat tcgctttgtc agcgcgatag
     4321 agagctattg cgcgaacggc atcccgaaaa cgaacctcta ttctctcgcc gtgcattact
     4381 acaaacactt catcctcggc tgggaataaa aaatctcccg gcggacacgt cgtccgccgg
     4441 gagattctgc attatagagc ggggtttgtt tgattcagta gaacgtcgca acttcctgct
     4501 ccggcacgtc gcacggctcg atggacacca tgtgcagcac cgggccgggg ccttcgtaca
     4561 cgtccggcag agcgcggatg tcgatgcggg cggtgagctt gtaccaggca aggtttttga
     4621 tctcttccgt atccgcggct tccgcggcga gcgggatgaa gcggatatcg ttcgcgcagc
     4681 aggtcatcac gtcgcgcccg aagtagaagt atcctttctt cagactgcgg gatttcgtga
     4741 tcgccttgcc cttgagagag acggtcttgt tccggtattt ttccggctga tccatgatat
     4801 cctgcagaaa gatcgcatag tccgcgtcgc cgatctcgca gacgggggcg ttgagatcgt
     4861 aggggagggg gagctcaatg tcgtcgtagc tgatcttgcc gtccggcgtt tcaaaggcaa
     4921 tgtcgcaccg gcggttgatg gcgcggatga tcttgtgcgc ctgcattttg agatcctcgt
     4981 cgccctcggg gaagcggttg agcaccgcca gctcgcagct ctggagcttg tccaccatga
     5041 gctggcgcat gttgttgttg tagttgagga acgtgcgcgc gtcgatgaac agaaattcct
     5101 gcgccacgat ccactccttc ggcatgttcg tatagagcgt gtcgagcgtc cacatgccgt
     5161 tgtactccac catgacgtag gagcagcggt gctttttcaa aagcgccgcg agattttcgg
     5221 tcgtaagatc gctctcgctc tcgatcgtct cggtgaacac ggtcggcgcg gcgaacgctg
     5281 agggatcgta ttcctctacg ccctcctcgc acacgagaag gaggatgcgg tcgccgttat
     5341 taaagctcgc gtcctcgagc gtctgctggg cgaacttcgt cttgccggac tccagaaagc
     5401 cggtgaagag atatacgggg acttgttttg ccatggctta ttctcccttt cagcggaaga
     5461 gctcggcaac ggcgtcctcg tgcagctcgt gaccgatgac gcacagccgc cccgtcacac
     5521 cggcgctgcc gcggcggatg ttgatctcgc ccggcgtgta gtcaaagtgg atccactcgc
     5581 cctcgctcgc ggcgacgatg cccttcgcgc gcagcacggc gccgtatttg gcctcgtcct
     5641 caagcgcggc gagcgcagcg cgcagaccgt cctcggtgaa cttcttcgcc gtttccatgc
     5701 cccaggacgt gaacacctcg tccgcgtcgt ggccgtggtg atggtgatga tggccgcagc
     5761 cgcactcgtg atcgtggtcg tgctcgttgt gatcgtgatg gtggtgctca tggtcgtggc
     5821 cgcagccgca ctcgtgctcg tggtcgtgct catcgtggtc gtggtgatga tgctcgtgct
     5881 catgcccgtc gtcgtcatcg tcatcgtcgt gatggcagcc gcaggagcag ttgccgtttt
     5941 catcgaaatg atgatggtgc tcgtggtcgt gatgatggtg atgctcgtgc tcatcctcgt
     6001 cctcttcgtg ctcgcgctca atgctttcaa gcgcggcggt gagcgtgtcg cgcttttcca
     6061 tcgcgtcgag gatctgcgcg ccggagagct catcccacgg cgtcgtgatg atgacggcgc
     6121 cgggattctt ctcgcgcagc agcgcgacgc actcggcgag cttttcctcc ttgatgcccg
     6181 cggtgcggga gaggatgatg cacccggcgg attcgacctg attgttgaaa aattcgccga
     6241 agttgcgcat atacattttg catttgcccg cgtctacgac ggtgcagagc gcgttgatcg
     6301 taagctcgcc gtccgacgcc tgctccacgg cggtcacgat gtcggagagc ttgccgacgc
     6361 ccgagggctc gatgaggatg cggtcggggg cgtagcgctc gcagacctcg tgcagagcgg
     6421 cgcggaaatc acccgtaagg ctgcagcaga tgcagccgga gttcatctcc gtcacctcaa
     6481 tgccggcgtc cttcatgaag ccgccgtcca cgccaatctc gccgaactcg ttctcgatga
     6541 ggacgagctt ttcgcccgcg taggcttccg cgatcagctt tttgatgagc gtggtcttgc
     6601 ccgcgccgag gaaaccggaa aatacatcaa cttttgtcat acatataacc tctttctgct
     6661 ggaagcagcc tgtttttcca atagaatatt ataacacgtt cgtcaagggg gtatttctgc
     6721 cattgcctga ctttttacgg aaaaatgaat tgtaataata taaatgtatg ctataatgaa
     6781 taaatgtccg ttttactcca ttccactctt ctatttattt ccgatccact cagcaaattt
     6841 ttgagaggta gacaaattgg aacagaaaaa gaagtccgcc cgcacgatgc gcgtgctcgg
     6901 cacgctgctt gagggtagcg gaaaatactt cgccgtgtgc atccttctgg gactgctgct
     6961 cacggtggtt gaaatgatcg cgccgcaggt catccgcgta acggtggact cggtgatcga
     7021 ctcgcagccg atggatctgc ccgcgtgggc gctccggtgg gtggagtcgc tcggcggcgt
     7081 ggcgtttctg cgcgcgaaca tgtggttcat cgcgctcatt ctcgcgcttg cgggtctcgt
     7141 cgcggcgctg ctgcgctatg gcgcgaacat gttcaacgcc aaagcgggcg aaacactcgt
     7201 caagaccgcg cgcgacaggc tcttccacca cattgaatgc ctgccgtgga agtggcacgc
     7261 cgagcatccg accggcgata tcatccagcg ctgcacgagc gacgtcgagc gcattaaaaa
     7321 ctttttccag gagcagttca tctccgtttt ccagatcatc gtgcgcatta tccttgcgct
     7381 gacgcttatg acgctgatga acgcgaagct ctccatcgtc gcgtgggtca tcgcgccgat
     7441 catcgtgctc tactccgtgc tcttccataa caagatccgc gcgcgcttca ccgagtgcga
     7501 cgagaacgag ggcgttctct ccacgatcgc gcaggaaaac ctcacgggcg tgcgcgtcgt
     7561 gcgcgcgttc gggcgcgaaa agtttgagcg cgaccggttc gaggcgcaga acaacaagta
     7621 taccgagtgc tggatcagcc tgacccggac gctctcggat ttctgggcgg tgggcgattt
     7681 tacgagctgc ctgcagatcc tgctcgtggt cgtgctcggc acggtctcgt gcgtgaacgg
     7741 caatctgacg gcgggcgaat ttgtgtcgtt ctcgctttat aacacgatgc tcatcggccc
     7801 tgtgcgccgt ctcggccgca tcatttccga aatgagcaag gcgggcgtct ccatcgatcg
     7861 tctcggcgag gttttggaag ccgagaccga gcaggacgcg cccaacgccg ccgaagcgcc
     7921 gatggacaag gacatcacct ttgaaaacgt cagcttccat tacggcgagg acggcccgga
     7981 cgtgctcgaa aacgtcagct tcaccgtacc tgccggaaag agcttcggca ttctcggcgg
     8041 caccggctcc ggcaagagca gcctgctgct tctgctctgc cgtctgtacg cgcccacgga
     8101 gggcaagatc accgtcggcg gcgtggacat tgccgatatg cccgcgcgtt atgtgcgcga
     8161 gaacgtcggc gtagtgctgc aggagccgtt cctcttctcc cgctcggtgc aggagaacat
     8221 cggcatctgc ggcgcgagcg acgcggagat ccgtgccgcc gccgtcaccg cctgcgtgga
     8281 cggggatatc acccgttttt cgcagggcta cgacaccatg gtcggcgagc gcggcgtgtc
     8341 gctctccggc ggacagaagc agcgcgtggc catcgcccgc atgctgacaa agaaaacgcc
     8401 ggtcatcgtt tttgacgatt cgctctccgc cgtcgatacg gagacggacg agaagatccg
     8461 ctcggcgctc gaccgtgatc tcggcggcgc gacgacgatc atcatctccc accgcatcac
     8521 aacgctgctc gactgcgaca acattctcgt gctcgaaaag ggccgcgtaa gtcagctcgg
     8581 ctccccgaag gagctgctgg cccagccggg cattttccgc aagatctacg acatgcagat
     8641 gtccatcgga gaggaggaag gagcatgaac gagcagaaaa agaccaaagg cgcaaaatgg
     8701 ttcggcatcc ccaacctcgt cccgtatatg agaccctacg gcaagctcat cttctggatg
     8761 gtactcatgg cgttttacgg cagtgccatg gacgcggtca tcccgctctt ccagcagtac
     8821 gccatcgatc attacatcgc cgactcgacc cttgcggggc tcggctggtt catcggcgcg
     8881 tatgtcgtcg tcatcctcac gcagacggcc tccaactata tctccgcctt cggcgcgtgc
     8941 aagctggagc tgtccgtcgg ccgcgatctc aagcgcgaga gcttcaacca tctgcagacg
     9001 ctctcgttct cctacttcaa ccagaacagt gtcggctatg tccacgcgcg cgtcatgtcc
     9061 gataccgacc gcatcgcgag cacgctcgcc tggagcatgg ccgagggcgt gtggcattcg
     9121 gcgtatctcg tgtgcgccat cgttatgatg ctcatcctca actggaaaat ggcgctgtgc
     9181 gtgctcgtga tcgtgccgat cgtggcgatc tcgtccatgt acttccagaa gaagctcgtg
     9241 ttcttccacc ggcaggtgcg cgagaaaaac tcccgcatca ccggcgcctt caacgagggc
     9301 atcaccggcg cgacgacgac gaaaacgctc gtcgttgagg acaagatgga gaaggaattc
     9361 gatgagctga ccgccgacat gaaaaaggtc agcgtccgcg cgatgcactt ccgcgcgatc
     9421 ttcatttcca caacaacgct cgcggcctct ctcgcgctcg cgctcgtcct ctggcgcggc
     9481 ggcatcgtca cgcgcgaggg cgtgatgctc atcggcacgc tcaccgtgtt tatgaactat
     9541 gcgcagggca tgatggagcc gatccagtgg ctcgtgcagg cgctctccag ccttgtgaac
     9601 gtgcaggtca acgtcgagcg cttcaccacg ctcatggaga cgaagtccga tgtgcaggat
     9661 acgccggagg tcgtggagaa atacggcgac acgttcagcc ccaaaaagga aaactgggag
     9721 gagctgcacg gcgatatcga gttcaaagat gtgaccttcc gctaccccga cggcgacgag
     9781 aacattctcg agcacttcaa cctgaaagtg ccgcagggaa cgaatgtcgc catcgtcggc
     9841 gagaccggcg cgggcaaatc cacgctcgtg aatctcgtct gccggttcta tgagccgacg
     9901 gaggggcagg tgctcatcga cgggcgcgac gcgcgggagc gcagccagct ctggctccac
     9961 tcaaacatcg gctatgtgct ccagacgccg cacctcttct ccggcacggt gctcgaaaac
    10021 ctccgctacg gccgcgagaa cgcgactatg ggggagatcg aagcggcggt cagggccgtc
    10081 tccgccgaag ggatcatcga gagactgccg gacggctatc agagcgacgt cggcgagggc
    10141 ggcaacagcc tatcgaccgg cgagaagcag cttctgtcct tcgcccgcgc gctgcttgcc
    10201 gatccgcgca tcttcgtgct cgacgaggcg acgtcctccg tcgatacggt cacggagcgg
    10261 ctcatccagg acgccatcga gaaggtcatg gacgggcgca cgtcgtttgt gatcgcgcac
    10321 cgcctctcca cgatccgccg cgcggacgtc atcctcgtgg tgaacgccgg ccggatcgtc
    10381 gagcgcggca gccacgccga gctcatggcg aaaaaaggcg tgtactacgg cct
//
LOCUS       DAWAAE010000068        10347 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2909_length_10347_cov_2.806549, whole genome shotgun sequence.
ACCESSION   DAWAAE010000068 DAWAAE010000000
VERSION     DAWAAE010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2909_length_10347_cov_2.806549"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2267..2324
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1967 a         3155 c         3140 g         2027 t
ORIGIN      
        1 gccactggga aaccgggaag gcgggcgagc gaggaaacgg agtcaggata tttcaccgga
       61 tggtatgttt ccgattaaaa cgctttgata ttctgaaaat accgtcagag cagcctttgg
      121 gctgctctgc ttttattttt cgggagctgc gtctctggaa gttcgttttg cgagattccc
      181 ccatgaatat tttcaggagg gcatggttat gcaaaaaacg ttcttccgag cccgcgcagc
      241 ggccgggctc atctctccgg acgcacacac ggaggggcaa tcgtcatgaa aacgggaata
      301 ggaatgtttt acgccgtggg cgtcggcccc ggcgacccgg cgtggatgac acgccaggcg
      361 tgtgatgttc tgggcttctg ccccgtcatt gccgcgccga gatccgcttc cggggaaatg
      421 ccggcgctgg aggtagcccg cggcgccgtc gatctgagcc ggaagtcgat cgtgccgctg
      481 aaatttactg cggcacgggc acggtccgcg cgaaacgccg agtatgtccg cgcagcgagc
      541 agcatcgcgc gcgtgctgga cgcgggctta agcgtcgcca tggtgaatct cggcgatgtt
      601 tcgatctgcg ccacggcgta cgacgtgctt gacgaactgc gccgccgggg gtacgccacg
      661 cagatgatcc ccggcatcac aagcttctgt gccgtggctg caacgctcgg caccagtctc
      721 accggcaggg actcgccgct gcatatctat ccggatgaaa cagggaatct ggacatggcg
      781 ctggagcttt ccggcacaaa ggtgctgatg aaatccggca aggccatcgg cgagaccgcc
      841 gcggcgctct cgtgccgcgg tcttgcaaag gaggcctcca tggtcgccga ctgcggtctt
      901 ccctcgcagc gcatcttcag cgagatgacc gatctgccgg agaacatcgg cgtctcttcc
      961 acggtgatcg tgccggaagg acacagagcc gcgcggaaaa agtgaataca aaatgcgccg
     1021 gaacgggctt ttctgttccg gcgcgttggt ttttatgctt tttttccgct gcggttcttt
     1081 tcccagagca ccgcaaggcc gatgagcagc agatccggct cgtaccggat ttcccggcgg
     1141 cagaacggcg tgacgcaccc ggcgagaccg ccggtggcga tgacggcggc gggagcgccg
     1201 agctccgttt ccatacgctc gatcatcccg tcgagcaggg cagccgagcc gtagatcgcg
     1261 ccggactgca tgcaggcgac ggtgttcgtc ccgatgcact ttgcgggcgc gtcgagcgag
     1321 acacgcggca gctgcgacgt attgccggag agcgattcca tcgacaggcg aagccccggc
     1381 acgatcgcgc cgccggcaaa gcggccctgc gcgtctacgg cggagatggt cgtggcggtg
     1441 cccatgtcga tgacgatgag cggcgggcgg tgcagatgca gcgccgcgac cgcgccgacg
     1501 acgagatccg ccccgatctg gccgggatcg tcgagccgga tgtccagccc ggttttgaga
     1561 cccttgccga caacgagcgt atccagcccc gtgaggagct ttaccgcccg gcacagcgtg
     1621 gacgtgagcg gccagacgac gtttgagagg atcgcgccct caaaactctc cggctgaaag
     1681 cccgcaaacg cgaggacatt tttcattccg acggcgtact cgtagtcggt cttattcacg
     1741 tccgtcgcca tgcgcgccac ggcgcggatc ttccggtttt cgatgcagcc gagaacgatg
     1801 ttcgtgttgc cggtgtcgat ggcaaggatc atggcttcag ttttcctttc tcccgatgcg
     1861 gatcagccgc agaatgacgg ggctgagaac aatgttgatg ccaagctcgg caaggaagtt
     1921 gacgccgacg agaccgatga tcataaaccg gcccacgtta tcgccgaagc cgaggttctg
     1981 cgccatatcg gcaggaagaa gatccggcag ccgagcagaa atacgccggt gttcacgacc
     2041 gggcagacga tggccgcggt gacggcggca agcgtgcggt ttttctgagc gagccacgca
     2101 tacacgagcc ccgccagacc gccgcaggca atgcccttta cgagcaccgt gagcagcgtt
     2161 cccggcacgc tgaaggcgaa aaacgccgcg gcgtcgcccg agagcagcac caccatgccg
     2221 aaaacaaagc cgagccacag ccccgccgcg ctgccgtata ccgccgnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgatc agcgccgtga
     2341 aaagcgccag acccgccaga cgccgggtct tttcctgaga gtttgcgttc atattcaatt
     2401 cctcctgctg ttgctccctt agtatgtgcc gtgcggcacg ggatgcagcg catggcatat
     2461 tataaaagca aagcccctct gcgtcaagca gagggggata ttttataaag aaaggtctct
     2521 tataccaatt gcataataat cttcttccgt aaaagcggaa atgggtttct ccatttctat
     2581 ggaatacctt cgcatggcag taaactcttt ttctccctgc ggattcggcg gcagcgtcca
     2641 cggaagcgca acatacccac cggcacgctc ggcagcctca tcaagctccc gcatttcctg
     2701 ccacttgcgt tcgtccactg tcaaccctcc tcaaatctca ggggatttag tcatttaaaa
     2761 gtgctccgtg atctccatgg ttccggcttt gggatcaaac agaaagaccg ttctgtggtt
     2821 cggctttgaa tttaaatccc cgcccatcca gattttcggt accccggacg ggtagtactc
     2881 cagcgggcgc ggggtccatt cgtctattgc aatctcgtac atattgttcg ccaaactgtc
     2941 gaaagaccag catatgtggt ccgcattttc ccgaacccat ttttcgcccc acagctcaat
     3001 atatttttct ttggcccgcc ttattgcttc ctttctggtc atactgtttc atctcccttt
     3061 aaagtccgct tttttgaggc agagcacctc gtagcgatca ccatcatact ttcggatgcc
     3121 aagacggtca agagccgcca ttgcaaggtc tttcacaagg aatgtattcc agaaacttgt
     3181 ctcgtcctct gcggctttca tcagcccgct ttctatctcg gcattcgttt cgccgcgttc
     3241 agcgatttcc atgattgccg aagctttgac aagaaagttt tcatggctca gcagaggtac
     3301 cagctcatcc gtttgcatct gtggaatgtg atccgtcaaa cagccgcctt tcatgcttct
     3361 ttctcctaaa attctttatt ttaagataga tgctccatga tatcgctgcc ggtgtccgcg
     3421 ccgacgagcg agcggacaag accaaaccgg cggcagtcgt agcatggcct ccgaagcaac
     3481 ttcaaacgcc gttttgccgc cgaagctctc cagcggcgtt tccacgtcga ggatgatcgg
     3541 gttttcgtcg gcaaagtgga gatacagctt cggcgtatcc catacgccgt actgcgcggc
     3601 gctctcggca tcgtacgaag catccgcggc gtacacgacg gcctgttcaa gcgcgagcgc
     3661 gttgagccgg tgcgcgccgt ggccgtattc gccctcgcgg tcgtgcccga cggcgacgag
     3721 cggcttgaac cgccgcagca gtcccacctg ataggcgatg acgttttcct cgccgaagct
     3781 ctgctgcgcc gcctcaagcg aaagaacgta atagtccggg aacggcccga tgaccggata
     3841 gttccggatg cccatcgtcc agagcgcgtc cagaagctcc tgcgggcgcg gctgccagtc
     3901 gtaatgattc acaagatacg ccacctgcac cgcgagcccc cggtccacgc actgcgcggc
     3961 gagcgcgccg aagaagataa cgtcatcgtc cgcgtggctc gggaggagca gcacatccgc
     4021 ctgctcgcac ggtggctgcc agacctgtac gtccgccggg gccgcgccgg tcgtaaagag
     4081 gcgcacctcg cagagcggcg agaggccgga aaactcgata tcaagctccg tcaccgcctc
     4141 cggcagcgcg agatactcgt gcagaaagcc gtactcgccg ccggggacgt cccccgcggc
     4201 ggtatggacc gtgaatgctt cgcacggggt ataccagtaa agatagagcg cggcaatggg
     4261 cgtttcggag gaaatgcgca gcgtgttccc ggcgcggtag tcgatgcggg aggtgcggtc
     4321 gccgtcgcgg aggctcggcg tgtagccgcc gatgctgtcc gagagggaga cgccgtccgt
     4381 gatctccgcc gcgaccggtt cgggcgtggg ttccggcgtt gcggtcggtt cgggcgtggg
     4441 cgtgggcgtc ggctccggcg tcgctgtggg cgcatccgtc ggcgcgggag tagccgtcgg
     4501 ctttggctgc gccggtgcgc cgcaggcggt gagaagcgcg cagagcagcg cgaatatcag
     4561 aagaagagaa atacgttttt tcgtggcggg tgtcctttct cccgcggccg gcgcggggtg
     4621 ggtttgggag tattatagga cgttttgagg caaagcgtca aggcacggtt ttcaaacgct
     4681 cccttgtgca aagggagctg ctgagcgaag cgaagctgag ggattgtagt cttcagaaag
     4741 cgacaatccc tccgtctttt gcctgcggca aaatccacct ccctttacac aagggaggca
     4801 ttaaaacggg aaagccccga agcataattt gctccggggc tctgtcactt atgattatca
     4861 gcccgcgttg ccggacgagg cgttgccgta ggaggcatag ttgccggcgg aggcataata
     4921 ggcgttgccg ccggaagcgt tgcccgccga ggcggtcttg gggccgcagc ccgcaagcgt
     4981 gagcagcagc gccgcggcgc tgagaacgat ggcaaggatc tttttcatgg atggtactcc
     5041 ttttcttttt gtcaatggtt gttattcttc gatatatcga tgcgcccggc gcggagcagg
     5101 aggagagata ccgcgccggg ttgcgcagca ggaaaattat gcaatggccg ctcctgcggc
     5161 aatggcggcg aagagcgtgt ccacttcccc gctctcccac ccggcggtga gaagccgccg
     5221 gtcgcgcaca agaccgagct cctgcccgga ggtgagcgct tccgccgcag tccgcaccgt
     5281 ttcaaggcgg gttttcagcg ccgcgtcgtt ttcgccgtag cgcgcggcgt aggccgcgcc
     5341 ggcggcgcgc gccgaggctg tgtcgcgtcc gtccgcgaga tactggccga ggacggattc
     5401 cgcccagcgg tacgcgccga ccgcgccgga ggcggggtca taatcctgcg caagccgctc
     5461 gagaatgccg ccgataccgg cgagcggttc tgtctcttcc ggcgcaggcg aaatctccgg
     5521 caacgatccc ggcgtaactg ccggggtctc cgtcggcttc gacgtggctg cggcggcgcg
     5581 cgattcaagc ggcagacccg ccgctgccat cgctcccgcc aggaacacca gtgtcaatat
     5641 aaggatgaca actctttttt tcattgtctt ctcctttcgg gaagcattta tccggcgagg
     5701 ccgttcacgt ccgtactgcc gtttaattcc acattgttca gcacattgtt cagctctccg
     5761 agagtctgca ccgtgacgcc gtgagagcgc agccacgcgt tcgtgcgcgc ggaggccgta
     5821 aacggacgga cgctgtaata attgtagaca tattcgccgc tgctgttggt gtggacgccg
     5881 gtggcggtga tgtagatcgg ttcgatcgtc cgatagaaat cctcgtcctg aatgatcttg
     5941 acgccgccaa tattgccgtc gcgcagatcg gggacgatac aggtgtcgta aagctcccag
     6001 aattcgtccg ttgtgagcgt gagccccagc tgcgtcggcg agtttgacac ataccactga
     6061 atgctcgcag aaaatacggc agtctcgctc cggtcgcacg ggaagagcgc ccgtccctcc
     6121 atcagctcgc gggagttgag aagctcttcg tactgccgga tgttccggct gattgtgtcg
     6181 ttctccgcgg ggaagccgac ggtaacgttg ccgccggtca catacggggg gtacttatcc
     6241 gcagcatagg tatacacgcg gcgcgcgagg agctttccgt ccgtgccgta atactcgatc
     6301 gtcagcggga aagtgttgat tccgttctga ttttcgtaag cttttttgtt tgcgataatg
     6361 tccttttgca gagagaggag cttttcgatg ttctccggct gtacgatgct cctttcattg
     6421 tattcgcccc agccgttcac cgtggcgtac ccgatctcat ccacatcggg ctgatagcgc
     6481 tcaacgccga acacatcgag ctcggtgacg acgacgagcg cgcagagcag cgcgcagaaa
     6541 acgccgagac ccttccagtg ccggtcaaac acgcgcagcg ttttgcgcat gagcgcctcc
     6601 gcgccgaacc agccgaccgc cgcgccgaag agcatggcaa ggagcaaaat tgctactctc
     6661 gccggagtgc cgtcgttccc ggcggcatag gagccgacat tgaatacagt tttgaggatc
     6721 agcagcgcca tgccgagcgc gcccacggcg gcaagccccc agcggaaaat ggggcgcagc
     6781 accggaacgg cgacgacctc gcccgcggcc tccatccgcc ggcggcggaa gaagatcaca
     6841 gcgagccccg cgaggatcac gcccgccgcg gcatagatgg ccgtgagggg gagcgcatac
     6901 gaatagcttc ccacatagtt gttgaaaaag taatagagcg gcgagaggat cgcgagctcc
     6961 cagccggtgc ctctcacgcc gaagagcaaa aactccgcta tgtaccgggt cacgacctcg
     7021 atcccgacaa aaccgtagag cgcgataaca tagagcgccg ggagcaccgc cgcgtttccg
     7081 gtgaacaccg tgcagaacga cgcgagaccg aagaacgcta tgttcatcag cgccgctgct
     7141 gccgccgagc acagaaggta cggagtgcac ggcgagcccg cgccgccgta gagaagcaaa
     7201 ctcacgagca ttgcgaaaat gcacggcagg atgagaagcg tcagcccggc gagcaaactt
     7261 gaaatgagaa gcgcctcgcg cgtcacgggc agcgccgata tgtatgctgc ggaggtcttg
     7321 cggaacatcc agccgtagac agcgaccgcc atcaccaccg ccgcggccgc cgcggcaacg
     7381 atctcccggc gcgtcaggtt gctgaggatg ctgtagctca catactcgct catttccgta
     7441 aactcggaat tgaaatactg cccgcacatg agcgtaagaa agcagagcac tacggcatag
     7501 gccgcaccga tgagccagaa gcgggagagc gcccgtttgt atatcgcctt attaaagaac
     7561 gttctttcgg acggcatcgt ccacacctcc cagctcgtaa atgaagattt cttccagcgt
     7621 cagcggcacg gaatccatga acagcgggtg cagcttttcg agccacgcga gattgtcctc
     7681 ttccctgccg cgcagtatga gcgtctgcag cttgccggtc gaggagcggt ggagaatgtc
     7741 cagtccctcc tccggcagcg ccgcgccgtc cggcagggcg agcagcacct tggtcgtcgt
     7801 ctcctgcaat tcggaaacgc tcttttccag caggcagcgg ccgcgctgca tgatgccgac
     7861 gtggtcgcac acgtcctcca gctcgcggag gttgtgcgac gatacgagca ccgtcatgcc
     7921 ctcctccgcc acatcctcca tgacaagaga ccacacctgc cgccgcatca cggggtcgag
     7981 gccgtccacc ggctcatcga gcacgagtac ctccggccgc gccgcaagcg cgatccagaa
     8041 ggccgactgc ttctgcatgc cgcgcgaaag gcggcggatg gggcgcgtat cgtcaagcgc
     8101 gaagatctcg cgcagctttt cgtagcgttc ggtgtcgaag cggggataga gcgcgcgcgt
     8161 aaatttcata agatcgcgcg tcgtgctcgt ggaggtgaaa tacggctcgt ccgggatgca
     8221 gagcatccgc gccttgaccg aggggttttc aaataccggt tccccatcga gcagcaccga
     8281 gcccgcgtcc tgccgcagca cgcccatcat gtgacggatg gcggtcgtct tgcccgcgcc
     8341 gttcgggccg atgagaccgt agaccgagcc ggacggcacc gtgagattca gcgcgtcgag
     8401 cgctttgaag ccttcgtaga tctttgtaac gtttttcagt tcaatgctcg ccatggccgt
     8461 cgcctccgat cttttctatg atctcttcct cgctcacgcc gagccagcga agctcgcgca
     8521 ccgtttcctc cagccgggcg agaagctcct cgcgccgcgg gtcgctctgc ggggagaact
     8581 gggcgcagaa gctgcctttg ccggggacgc agacgatgta tccctcgctc tccagctcgt
     8641 ggtaggcacg ttggatcgtg ttcgggttga tggcgagcga taccgcaagc tcgcgcaccg
     8701 aggggagctt ttcccccggc tgcatcgcgc cggtgacgac aagccgccgc agatcgtcct
     8761 tgacctgctc atagatcggg cgcccgtccc ggtaattgat cgtgaccatg ccgccacctc
     8821 cttccgtgta tcaactgtac tacgaaactt aacacagtta atacagtttg tcaagcccaa
     8881 aaaacaaaaa gcctcccctg tgtaagggga ggcgacgcgg cgaagccgcg gcagaggggt
     8941 tgtctttttc cggcggctac aacccctcag tcagcctgac ggctaatagg ggaggtggca
     9001 aaaccggagg ttttgacgga agggtttctc cgccggaaga aggcaatccc tcagtcagct
     9061 tcgctgacag ctccctttgc acaagggagc cttattcagc cggatacttt atcgagttcc
     9121 ctctcgcagt cgggcatttt cctgactttc ggatcacgga agagcttgcc gcgcatcgtt
     9181 accacggaca catggcgcag cgcctcaagg ttttcgagcg gatttcccgc cgtgacgatg
     9241 agatcggcgc tcttgccggg cgtgagcgag ccggtgaggc cgtcgatccc ggcgatcttc
     9301 gcgttgccga gcgtcgcggt gtgcagcgca aaggcgttgg atacgccgca gaacgtgtgg
     9361 aaatagcgca cctcgcgcca catgtcgtag tgcgtgatgt acgggcagcc cgtatcggtg
     9421 ccgagaccga cggggatacc ggccgccaga cacgtcttgg cgcattcgat gatgccctcg
     9481 agcacgacct cgccgttata ctgctccatt tcggtcgctt tgctgacgct gcggtcaaag
     9541 cgggcatacg gcaccgcggc ggagagcgtt gctacctgtg ccgcgccgcg ctcgcggaaa
     9601 aggcgcagaa tttcctcgtc cggcttcgcg ccgtgctcga tcgtatccac gccgttttcg
     9661 agcgccacgc gcacgccctc gggactttcg acatgcgccg caacgggcag gccgagcctg
     9721 tgcgcctcgc cgcacgccgc acggacgtac tccggcggca ttttcagctc gcccggctcg
     9781 ccctttttct ttgcatccag cacgccgccg gtgatcatga gcttgatgag atcgggtttt
     9841 ccctgcgcga tttttcgcac gtcctccgcg gcctgcgcgg cgctcgtcgc ctcgtaagcg
     9901 agcgagcccg ccatatgccc gccggggacg gaaacggcca tgtccgcggc gagaatgcgg
     9961 ggcccgtcgc acttacccgc ggcgatgcgg ccgcgcagac gcgtatcgat gtccgcgatg
    10021 ccgccgacgg tgcggatcgt cgtaacgccg ctcaaaagct gcgttttggc gtagccctcg
    10081 cacatggcga gcgcaatgcg ccgcgtgagc gccatggacg tcatgaattt tacgagcttg
    10141 cggggattgc cctgcttttt tgggctgccg ctcgcgggca gatgaacgtg caggttgatg
    10201 aggcccggca tgagatatcc gcctttcaaa tcgacgatct caaagctggt gagatccgtc
    10261 ccctcaggga cgatatcgag gatcttctcg ccgtccgtgc gcagcaccat gccgcgctgc
    10321 ggctgcatat ccgtgctgcc gtcgagc
//
LOCUS       DAWAAE010000069        10260 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2940_length_10260_cov_2.855855, whole genome shotgun sequence.
ACCESSION   DAWAAE010000069 DAWAAE010000000
VERSION     DAWAAE010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_10260_cov_2.855855"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    526..535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2122 a         2997 c         2931 g         2200 t
ORIGIN      
        1 atagcgccgc ggacctgctg gtcagcgtga cgggagtcaa cgcccagctt cacgtgcagc
       61 tcgacggtct cgtcgaactt cgccttggcg gtcttggtga caagctcgaa agcctcagcg
      121 ggctcatact gggcgctttt atcgatgagc ttcgtgctct ctttataatt tttacctctg
      181 aacacttcat tatcctccta aaatgtggtt tttcggaagg ttacttcctc ccacgtttgc
      241 cagctcagtc gccgacgacg acgcccatgg agcggcaggt accggcgatc atgctgacgg
      301 cggactcgat gtcggagcag ttgagatcgc gcatcttctg ctcggcgatc ttgcggacat
      361 cttccttact gatggtggcg accttgtcac gctgaggcac gccggaggcg gtctcgatgc
      421 cgcaggcctt cttgatcagc agagccgcgg ggggcgtctt ggtgatgaag gtgaagctgc
      481 ggtcggcgta aacggtgatg acgaccggga tcataagacc catatnnnnn nnnnnccctt
      541 ggtacgctcg ttaaagtcct tggtgaactg ggaaatgttc acgccgtact gaccgagagc
      601 cgggcccaca gggggagccg gagtcgcttt gccggcggga acctggaatc tgacatagcc
      661 aactattttc tgtgccataa ttaagcacct ccagaattat tcttctttta ccggttcgac
      721 ctggtcgagt tccagatcga tcggcgtctc cctgccgaac atggagacga tgaggcggac
      781 gctgttcccc tcggggtcga tctcgtccac cgtgccgagg aaaccttcca gcgggccgtc
      841 gatgaccttg acggtatcgc caagctcaaa gcccacggaa atctctctgc gctccacacc
      901 gagatcgagg atctcctgct cggtcagagg aacgggtttt ccgtcggagc cgacgaagcc
      961 ggtgcagccg cggctgttgc gcacgagatg ccagctttcg tccgtgagca ccatcttcac
     1021 cagcacgtag ccggggaata ccttgcgctc aacttcctta tcaccgccgt cggtatgctc
     1081 aacgaccgtc tccagaggga tgttgacctc ctgaatgaga tcctgcatct ggcggttttc
     1141 cgccgccttc atgatggaag cggctaccgt gttctcataa ccggagtatg tgtggaccac
     1201 ataccatttt gcgttctctg ccatccggct tacaccaatg tgatcagcgc ttcgacagcg
     1261 gccttggcca gagagtcaaa gccccagagc acgagagcgg cggcggccat aacgaccagc
     1321 gcgaccagcg tgttcttgcc gaactgcttg ggggtgggcc agacgacttt tttgagctcg
     1381 ctcttcattt cgcggaacca cttggcaacg cgcttgaaaa agccgagctt cttgtccgtc
     1441 cgcttgacgg ctgcgccctt cgtctgaggg gcgacgggtt tcttttcttc gttagccatt
     1501 ggatgcatcc tttctcacgt ctgtcattac ttcgtttcga cgtgcttggt gtgcttgcgg
     1561 cagaaggggc aatacttatt catctcgagc ttttcgggag aatttttctt gttcttcatg
     1621 gtgttgtagt tccgctgctt gcactcgctg catctcaggg tgatcttgat tcgcgaacct
     1681 gctttcgcca ttatttcggc acctccttgt aaaatttacg cagcgctttc cgcgcgcgta
     1741 tgcctccctg tatgggccga aagagcataa aaaataaacc tctcgaccga caggtagggt
     1801 taatataaca caaactgcct gtccggtcaa gggaattcaa cgaaaaaatc tcggtttttt
     1861 ggcgtatttt ctctgccgcg ccaaggaaaa acgccccggc gttttccgcc ggggcgcagg
     1921 aaagggacag ggttattttt cgccgtcttt cggggtttct tctgcgggag caacgctctt
     1981 cgcggcggct ttggccgcgg ccttctgcgc gcgcttttcg gctttcgcgg cacgctttgc
     2041 cgcccgcttt gcggcacgct ccggacggtt ctttgcgcgg cggcggatga gccacacgat
     2101 cagcacaacg actgccgcga gcggcacgag ccacgggagg ctcgtcacga accagaggaa
     2161 cagatcctgg aagaactcgc ccacgtcctt gagcgattcg gaaaagccgc gggtcatgcg
     2221 ctcccaatag gtgagcgtga tcgtcggttc gggcgtatac ttggtcacct cggaaacgta
     2281 gagcgacacg gtggagtaat tcacctgatg atcgtagtag cggagctgac cggtgagcga
     2341 gtcgatctca tactgcacgt cggtgagctg ctgctgaatg gcgagcatat cctcgaccga
     2401 tttggcaacg gcaagcatct caagaagccg ggtctcctgt gttttatagg cttcaaggcg
     2461 gctctgcacg tcgtaatact ccatcgtcac gttctgcatg tatgtgttgc agtagggaac
     2521 gttgccgaat tcggagagag agccggtaag cacggagaac ttgtcgctcg gcacacggat
     2581 gaggaaggac gcatagcgcc cgcccgtatt gccgcgggag atgctatagt agttgttgcc
     2641 ggaaatggtc gagctctcca taaagccgcc cagctctttg acaagtgcgc ggatctcctc
     2701 gatcgaatgc tcaaactcgg tcgtttcgag cgtcgcgtcg gcgctgtaga tgattttctc
     2761 gctcatgtcg gcgatatcgg ctgccgagtc tctgacatct tcctcataga agccgccgtc
     2821 ctcggcggca agattgccga aacctccggg ggcgctggcg ggcgacggca tcgactccga
     2881 ttccatcggc gtgtccggcc ttggctcctc catgggggcg ctgctgattc tttcgctgta
     2941 gcaagaatcg gtcatgctcg ccgttttcgc tccgcatccg gcgagaccga gtgcaagcac
     3001 aagagcgagc gcaagcgcaa ggagcttcgt tgtccttttc atcgggtgta cctccttcaa
     3061 aagtttattt aatgcttcta atactaagac ggcggcactg gaaaaaaagt tccggcatac
     3121 aggaaaaaga gaggaaaaat cctctcttac tcccgatata ggcgcggaat gctctccttg
     3181 agcatggtga tgacctcgca gggatgcccg ccgtacagcg tagcatagtt gccgacggac
     3241 gggacatcgg ggccggcgcc gaaaacggtc acctcgtcac cgagctctgc gtccggaatg
     3301 tccgtgagat cgagcgcggc gaagctcatc gagagcttgc cgatcacatc ggcgcgctgt
     3361 ccccgaacgg acatccatgc gcccgcgcac agcgagcgga tcagcccgtc gccgtagccg
     3421 acacccgcga tgccgacggt tatatcccgc ggcgcgacgt aagtcatcca gtagccgata
     3481 ctcgtcccct ttggcacgcg ctttatttgc agcagacggg tcttgaagtg cgcgatgctg
     3541 cgcacgccga gggcatcgta tccggcctgg atgccgagca gcgccgaggt gagacggaca
     3601 tagtccatcc gatactccgg gtgggagagg aagagaagac tgctctcaca gtgcattttc
     3661 agccctaacc catcaaaccg gcggccgaag caccggaaca tttcgagctg ttccacgttg
     3721 cggtagtcca tttccggctc catcatgccg gagatgtgcg tcatcagcgc ctcgttcgtc
     3781 agatggcgga gcgagagaat ggagcgcacc tcgcgctcgg cctcatccgc ccgcccgggg
     3841 acgggaatgc caagccgcga taggccgacg tcgagcttta tgtgcgtgcg cagcacgccc
     3901 cgggcgtttt ctgcctgcgc gtccatgagg cgcgcgtgca cggcgtcaac gacggggacg
     3961 atgatatcgt tttccatggc ctccggcacg cagagcgggt cgatcgggcc gagcagcagg
     4021 atcggcgctg tgatccccgc gcggcgcagc tccaacgctt cggcaagatc ggcggtggag
     4081 agcatccggc agccgcagcg ctcaagctcc tgcgcgatgt gtaccgcgcc cagcccgtag
     4141 gcgttggcct tgacaacggc gatgggcatg catccgtcgc tgcacatgga ttctatgatg
     4201 cggtaattct gccgcagcgc ggcaaggtcg atctccagcc agcagcggct ttccggagtg
     4261 atgatgggct tcatgtttcg tatcccttac cctataaata ttaaaatata aatttatata
     4321 aaacaacgtg gttatgtttc tttacaggaa attatccgcg cacggtgtca caccgtcgtc
     4381 ctctggagca tcaccgtcgg cgatgcgttc ggacggggcg ccggcggcca tctcttcggc
     4441 gagctttcgc tcgagatagc cgttgtaagc attcatgaat tcgagcgcgg tgtccagtat
     4501 ggcggggtcg agcgagtcga aaaattcgcg gtcgcgctgc tcccagttca agtggcgcag
     4561 tcggtgctgg cggaacagct cgcggccgta aggcgttaac ttatagtaga tctccttctg
     4621 gttgcccgga agctggtagg aaatggcggc gtctttggcg attagcttgc gaagaatttt
     4681 ggaaacggcg ctgcgcgtga gttccatttc atcggcgatg cgcgctgcgt tggggtgctt
     4741 catgcgcccg atgcagtcga tgcagttgag ctccgagttg ccgtacccgc tgagaatctc
     4801 ccgcgagtgc agcttgctca tgatatcggc agagttcgca tagcgtcgga attgttcaag
     4861 gatcaattcg ctttttgtca tggcggcctc ctgtggtaaa cctatatatt tgatattatg
     4921 gtatcatctt tttttcaccg tttcaatagc tgcggagaaa attgtagaat gtgtccttga
     4981 tgatacaatg tggaaacaaa aaattccgga tattagcgga cgctaatcac aacacgaagc
     5041 ttaaaaacgt aataatatta taatatatgt tgaaaaacag acatgccaat aaaatgaaac
     5101 ccaaaatcat gccgcgatac ggattcaaaa ataatgttaa ctaggacaca aaagtattga
     5161 caaaatacga agaaacgcct atagtacgac ccatagagat cggggaaacc gatacggtgt
     5221 ccccgctgta ccgaaaggag atacggcaat gcccaagacc cctatcacag caaagttcat
     5281 ccgcgtgccc gtggaaggcg ggcgtcttcc ggtagcgccc gcatttgacg attttctgga
     5341 cgcaaaacac atggtcgtcg tagagctcgg catgcagaag ggccatctgc tgccggagta
     5401 ttttgatgcg gagaagagcg caaagatggt cgaggagatc aacgccttca acgccgcctg
     5461 tcgcaaggcc ggcattccca ttgtgcatgc cggatacaag ttccgcaagg gcgggctcga
     5521 tctggtgaac gcgcagtacc cgcgcatcac gccgctgtcc ggcaaaaagc ctttccccaa
     5581 ccctgccatg gaggaagaca ccgatctctg gcagtttgcc accgaggtcg ccgagggaga
     5641 ccacaagctc ctctccgcca agcgccacag cgcctttgag gggacggacc ttgaattcct
     5701 tctcaaaatc ctcggctgca aaaccatcgt cttcgtgggc gctgggctcg actgcatcgg
     5761 tatgggcacg ggcttttacg gcatgatcaa ggacttcaag tgcctcgttg tcgaagaccg
     5821 gttctacccc tattttgaag atctgggcga agagtgcgcc aaggcctgca cgatgttcat
     5881 cggactggtc gtgcgcgccg atgagctgac tgccgaaatt caggcgcagg gaaaggagta
     5941 acgcgcatgc ctcgtactga tatgaagatc aacatcaacg gccaaatggt cgcgctgccc
     6001 gagggctttg acgattacat cgactggtcc cgtgcagcaa tcgtcgagct ggacatgcac
     6061 ggcggccatc tcgatcccga gccggactgc cccgcgccga gcgcccgcgg ccgcgacatc
     6121 atcgacgacg tcaataagtt caatcaggcc tgccgcgatc tcggcattcc cgtcgtacac
     6181 atcgtaaaca cctaccgccg cgagatcgac gagggaaagg aatctgcctg gcgccgcatc
     6241 actgagggac attactctct gctcggcgag aagtacgtcg ccatgacgaa gaacttctgc
     6301 aatctggcgc tggaggacac gaagtggagc acgctcgttg tggagaacaa gcccggcgac
     6361 gttgtcgtga acactaaaaa gcgcgtcact gcgttcgaga cgaccgatct cgatcttgtc
     6421 atgcgtgctc tcggcaagga cgttatggtc atcaccggca tcatgacgaa ctgttgcgat
     6481 ctctgcacgg cctactacgc gggaagcaag gactacaagg tcatcctctg cttcgacatg
     6541 acccgcggct cgaacccgag cgacgaagct gccgcaggac tcatgttctc ccgctacatc
     6601 ggcctgaaca ccaactccga agaactgctt cgcgagtggc gcgcgcagaa ggcgtaaaaa
     6661 ggcgcattga gccgaaccgg ctttttgtga atagctgcgg tcaattatgg taaaagaagg
     6721 ggctccgccg cctccggcgg agccatagaa attcgggata atccgcttgt cccgcgaaaa
     6781 agaaagaaaa gagaggttct gttccatgag ttcgaccaaa cagcccagag agaagaaaaa
     6841 ggcttctctc ggtttctcca tcctcacggt ccttgttctc atcgcgttta ttactctgat
     6901 gctcacggtc ggcaaggcgc ccatcgtcgt tgtgatgatc cttgcctggc tagttcttgt
     6961 cccgttcgct ctctatctcg gctacagcat gcatgagacc gagaaattcg ctctcgatct
     7021 catcaagccc gccatcggcg tcattgctct gctgatcgcc gtcggcggca tggtctccat
     7081 ctggctctgc gccggtaccg tccccgccct gatttactgg ggactgaaga tcatctcccc
     7141 gggcgcgttc cttctcgttg cgttcatcct tacatccatc gttgcgctgc ccaccggcac
     7201 gtcgtggggc accgtcgcta ccatcggcgt cgccatgatg ggcgttggtc tcggcctcgg
     7261 ccttcccgcc ggcgttgtgg ccggcgcggt catctccggc gcttacttcg gcaacggctt
     7321 ctcgcccgta tccgatgcac ccctgatgca gtcttccgtg ctcggcatcg acatctggga
     7381 ccacatcaag tttatggctc ccaccactgg catcgcgtgg ctcatctctg cggttctgta
     7441 cggcattctc ggcgcgcgct atgccggaac gtccgtggat ctcgcgagcg tcaacgacat
     7501 gcttgccagc ctcgaatcgc tcttccacat caacgttgtg acgctcatcc ccatggcggt
     7561 cgttctcgtc atgatgatca tgcgctattc cgcgctgatg agtatcctct gcggcagcgt
     7621 cgtcggcatt tttgtcgccg tgttcatgca gggcttctcc gtggccgagg tcaccggctt
     7681 catgaagagc ggcttctccg tcacctgcga taacgccctc atctcctcgc tcctcaatcg
     7741 cggcggcttc acgagcatgt atgagctcgt tgccatcatc gtcggctcgc tcggcatcgg
     7801 cggcatcctc aagggtacgg gcgtgctcaa tgtcatcatc gaatcgctga gcaagcacat
     7861 caagagcctc gccggtctca ccatcaccac gttcgtcgct tcagtcatca ccaacgcgat
     7921 ggtcggcact tactacttct gcatgacctt cgtcggcacg ctcatgcccg agctctacaa
     7981 gaagcagggc tacaagccgg aaaaccccgg ccgcatcatc aacaacgtcg ccaactgcct
     8041 cgttgtgttc ctgccgtgga acatcggcgc gcagtacatg tcctcgcaga ccggcgttgc
     8101 catcaaggac tttgctcctt tcgccttctt cccgatcctc atcatgctcg tcgatctcat
     8161 cttcggtatc ttcggcatcg gcgcggccaa atacacgccg gaggagatgg cacagttcac
     8221 cgcggaggaa gcggcggtaa aagcagaata aaaccattgc agtctttccc gttccataac
     8281 tgaccgccgg agaggtgggt ttatcccccg cctctcccat tctgccgtga tgggagactg
     8341 tcccagcatc ttgagtttat atattaagga ggtatcgctg tgaagatcac cggaatcaag
     8401 acctattggc tcaaagtccc ctacgataaa ccctttgaac cgacgtggta ccccggaaag
     8461 cgcgaaacgc accagcacat cctgctcgta cgtgtcatga cggacgaagg gatcgagggc
     8521 tacggcagcg gcgaatttcc cttcggcctg acgccgacat atatgacgtt tattcaggac
     8581 atgattgccc cctggctcat tggacaggat ccgctgatga tcgaaaagct ggccacccgt
     8641 ctcaagggcg acggacggct cgccccgcgt ccgtggatcg tggagaacgc cctctgggac
     8701 atcctcggta agaaggcagg cctgccggcg tataaagtca tgggcggcta tcgtgaccat
     8761 atcccggcct acgccgccat gtgcgagctg cgcagcgacg aggaacggca ggaggacgcg
     8821 ctccgcctgt tcgacgaggg cttccgcgcc gtgaagctgc gcctgttcat gccgacgata
     8881 aaggaagata tccacatcgt ggagaatatc cgtaaggccg tcggcgataa gatgaccatc
     8941 atctgcgatg ccaatcaggg ccccgtgcgc gaccgaaact ttgagggcgg gcgcgtcccg
     9001 ttctggacgt atcagcgcgc actcgaaacg gcaaaggagc tgcacgatct cggcgtgctg
     9061 tggcttgagg agccgctgga ccactacgat atccacggca tccgccgtct cactgaggag
     9121 acggagatag ccatcgccgg cggcgagatc atgaacggca ttcaggacat cacgacgctc
     9181 atcgacaacg agtgctacga tatctacatg ccgaacatca cgctctgctg cggtatcagc
     9241 catgtgcgca agatcgccgc gatggccgag cggcggggca ataagctcgt caacatccac
     9301 ggctgggtcc ccggcacggg cgtagccgcg gcactcgcga ccatttgctc ttacccgaac
     9361 gccacatggc tggaagtgcc gcacgatcct cccgccatca cccctgacag ctttcagggc
     9421 atcgtgaagg aaccgctccg catcaacaag gcggacggct gcctgtactg cccggataag
     9481 cctggcttcg gcgtggagct ggatgaggac aaggtgaaag cctacacagt tttggaaaaa
     9541 taaccggaag tggaggcgtc ccgccggacg ggaacgccgg aacaaccatg gttttctttt
     9601 caataaatcc aatgaaaaaa ggaaaacgaa aatggttacc gtaaacttta ttcttaacgg
     9661 caagccggtc agcgcggaag cggacgggct cacgctgctc aaatatctcc gcggcgtcgc
     9721 gtgtctgaag ggcgcaaagg aaggctgctc caccggtcac tgcggcgcgt gtaccgttct
     9781 cctggacgga aagcctgtcc gctcatgcgt tacccgcatg gacaagctgg aaggaaagtc
     9841 cgtcgtcacg attgaagcac tgcacggcgc cgatggcggc ctgcacccga tccagcaggc
     9901 gtttctcgat atcggcgcgg tgcagtgcgg tttctgcacg ccgggcatgg tgctcgcgac
     9961 gaaggcgctg ctagatgtaa atccggaccc gacggacgag gagatttgcg aagcgctgaa
    10021 aaacaactac tgccgctgca ccggctatgt gaagatcatt gaggcggtgc atctggcggc
    10081 ggcgcgcctg cgcggggaag acccgtccgt ggatgatatg cggacgtggg aaaatctcga
    10141 gatcgtaaaa tacgagggcg aggacgacgt gacggcgcag ggcctcaccg gccgccacat
    10201 cggccgctgc gccatcgaca tcgacggtcc ggccaaggtg acgggcgcgc tgccctttac
//
LOCUS       DAWAAE010000070        10158 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_2970_length_10158_cov_2.838860, whole genome shotgun sequence.
ACCESSION   DAWAAE010000070 DAWAAE010000000
VERSION     DAWAAE010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2970_length_10158_cov_2.838860"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2335..2404
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4961..4970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7987..7996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2117 a         2852 c         2970 g         2129 t
ORIGIN      
        1 cagcgggaac gttcattcag ggcgcgtcgc ttgagctttc gtgcgacggc ccggtgtgcg
       61 agccgtatct cggctttttg cagggcgggc ttacgtatga atccggcaag ctcggcgtgc
      121 tgagcgcctt gtctgaaatg cttgcctgac gcatatactt ttgggcataa gggggtgtgt
      181 tcccgtatgc cgtttccgcg aaaaatcaca ccgcagcaga cgaccgcact gctgctcctt
      241 gcgctcaccg gcgcgctgat cgcgtttctg atccccgccg tgcgctattt tcggacgctt
      301 accggcgcta tggcggtatc caatgcatcg gatctcatca cgcagaccgt gagcaacatt
      361 gtggagaacg aaatgcgcct gctcgggccg gacggcggaa aattcgtatc ctttgaaaag
      421 gacggcgacg ggcagatcag cgctattgta accgatacgt cgcgggtgaa tatcctctcc
      481 gccgagcttc tcaacgccat cgtggaggct tccgaccggg gagatcttga tctttcaatc
      541 cccttcggga acctgcttgg catcagcttt ttccttggcc gcgggccgac cgtaccggtc
      601 aagatcacga tgctctcatc ttcacgggtg gatttccgca atgtgctgac ggatgccggc
      661 atcaaccaga cgaaacacca gcttcttttg gaggtttgtg ttgatgcaga cgtcctgctg
      721 ccgtgggaga tccgctctac gcagatcgtc acggaagtgc tggttgccga aacgatcatc
      781 gtcggacgtg ttccgcaaac ctatttctat acgggagaga catgatggac tatttacagg
      841 agatcgccga gctccgcgaa gagctgcgcc gccacagcat agcctattac gacaaggatg
      901 cccccacgat ttcggacttt gagtatgacg cgatgatgcg ccgtctggaa aatctggaag
      961 cggagcaccc ggagaccgtt acgccggact cgccgacgca gcacgtcggc ggtcaccgtt
     1021 cggagaaatt ctcgcccgtg caccacgccg ttccgcttga atcgctcagc gacgtttttt
     1081 cctatgagga gctgaccgac ttcatccgca agacgaacga aacgctcggc ggcgaaccgc
     1141 tctatacggt cgagccgaaa atcgacgggc tttctatggc gctggaatac cggaacggcg
     1201 tcttctatca gggcgcaacg cgcggcgacg gcgttacggg cgaggacgta acggagaatc
     1261 ttcgcgtcct gcgcaatata ccgctcaagc tggaaaacgc gcccgagcgg ctgatcgtcc
     1321 ggggcgaggt gtatatggct cgggaggtct ttcttgagca taaccgccgc cgcgagatcg
     1381 agggcgaaac gctcctcgcc aatccgcgca acgccgcggc cggatccatg cgtcagctgg
     1441 acccgaaggt gaccaaagaa cgccagctcg acatcatcgt tttcaatgtc cagctcacct
     1501 ccggcgaaca gccgaaaaca cacgcggaat cgctggatat gctggaaaag ctcggatttt
     1561 atacggtgcc gcataaggtg ttccgcaccg tggaggagtg ctgcgaggag gttcggcgca
     1621 tcggcgatac gcgcgagagc tatccctttg atattgacgg cgctgtcatc aagatcaacg
     1681 accttgcgca gcgcgaaatt ctcggcagca ccgccaaggc gccgcgctgg gcggtggcgt
     1741 ataaataccc gccggaggag aagccctctg tagtgcgcga cattgtcgtg caggtcggcc
     1801 ggaccggcgt tttgacgccg aaagccattg tggagccggt gcgtcttgcc ggtacgaccg
     1861 tcacggcggc gacgctccac aatcaggatt ttatcgataa gctcgatgtt cgcatcggcg
     1921 atacggtcat cgtgcgaaag gcaggggaga tcattccgga agtcctccgc gttgatctca
     1981 cgaagcggcc ggagggaacc gtaccgttcc gcctgccgga tacctgcccg gagtgcggct
     2041 cgccggtgga gcgcgacccg gacggcgcag cggtgcgctg tatgggcgtg gaatgtccgg
     2101 cgcagaggct ccgcaatctc gtccatttcg cttcccgcga ggcaatggac attgaagggc
     2161 tcggcttctc gcttgccgag gcgctggtga acagcggcat ggtgaaaacc ccggcagatc
     2221 tctaccggct cgatgcacag accgttgcga cgctcgaccg catggggaaa aagtccgcgg
     2281 aaaacctcat aaacgagatc gaaaagagca agcagcggga tctttcccgc cttcnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnngtgctt gcgcgcacgt tcggttccat ggacgccatc gaaaatgcca cgccgctgga
     2461 gctgactgcc gtggacgata tcggccctat caccgccgag agcgttgtct cctgggcgca
     2521 gaacccgcag acgcagcatc agctccgcat gctgcgcgag gcaggcgtca acatgctcag
     2581 ccgcgaggag cgccgcgaca accgcttcct tggcaaaacg ttcgttctta ccggcacgct
     2641 cacgaagtat acgcgcgacg aggcgagcgc catcgttgaa tcctttggcg gcaaggcgtc
     2701 atcctcggtc tccaaaaaga cctcgtacgt cctcgcgggg gaggccgccg ggagcaagct
     2761 ggacaaggcg cgtacgctcg gcatccccgt catcacggag gatgagtttg aagagatgat
     2821 ccgctgaaac ataacagcag ccccttttca tggagagaaa agtatgagca cgagaaaaga
     2881 aatgataacc gagttctacg accggagcga cgagaacggc cggctggaga aaagcaggca
     2941 cggtcagctc gagtacgcga tcacgatggc ctacatccac cgctatttga accccggagc
     3001 cagggttttg gaggtcggcg cggggactgg tcggtattcc attgcgttgg ctaaagaagg
     3061 tttcgatgtg accgcggtgg aatttgttga aagcaacctt gccgctctta gagaaaatag
     3121 ctgcggagtt gatttgatcc gttcctatca aggcgatgct acggatcttt gtctgtttga
     3181 cgatgactcc tttgatatga cccttgttct cggccccatg tatcatctgt ttgctccgga
     3241 agacgtgaac cgggcaattg acgaggcgat ccgggtcacg aagccgggcg gcacgctcca
     3301 tttcgcgttt ctttcgatat acgcccttat gtactccaat gccctctacg gcagctgggc
     3361 gccttatcag gaagaaaact tcacgaagga tttcaaggta aggcactttc aggaacagct
     3421 gttcaccggt tacgatgtat acgagtttga gcgcctgttc gatgaaaaac cggtagagcg
     3481 cgtcacaacc gctgccatgg acggtccgct ggaggcgctg gagaaacgtc cggatttctt
     3541 tatttcggac gagaattttg acgcgtttgt taagtggcat cttgcgtttg ccgaaaagcg
     3601 ggagcttttg ggcgggtcca accatctgct gtatatctgc caaaagacga agcgataacg
     3661 atgcataacc tccgcatgag aactcatgcg gaggcttgcc gtctttgctt caggcgcagt
     3721 ttgtcctgct gcttatttta aaactttaag actcttctca tatttttctt ataattgttt
     3781 tgtatagtat tatctgaaaa tattgctttt ttccgcaaag tcggataatc tgaaagcgac
     3841 tttaaccgag cctgggggac ctcttttgga gaacaacatt ctgaaaaaaa tatcgcggca
     3901 gatcgtggat aagcgcgtga tcatcatgat cgcgtttctg gccgcgtgcg tttactgcgc
     3961 gctttccatc agccgcgtgc atgtgaataa cgatataacc tcgttccttc cgccggatac
     4021 cgatacccgc cgcgggctca cgatcatgga gaacgagttc acgacgtatg caagcgccaa
     4081 cgttatgctt gccaatacga cctatgagcg cgcgagtgcc gctgcggaga agatcgagac
     4141 gctcgatcat gtgaccggcg tgacgtttga taattctccg gcgcattttg tggattccgc
     4201 agcgctgctc acgatctcct ttgacggcac aagcgacgat gaaaacgtca ttgcggcgat
     4261 gaatgagatc cgttcgctca cagcaggctt tgatacatat acatcctcgg acattggcgc
     4321 ggacctcctt ggggagatcg cgcagcagat ggttggcgtc gtggcgctgg cggcggtggt
     4381 catcatggcg gtgctgctct ttacgtcacg cagctatttc gaggtcgtca tttttcttat
     4441 cgtcttttct gttgcggcgc tgctcaatat gggcacaaac ttctggctgg gcgagatatc
     4501 ctccatcacc aattccattg ccgttatttt gcagctcgcg ctcgccatcg actatgccat
     4561 tattttctcc catcgctatc aggatgagat cgaccgcttc ccaacggaac gcgaggcgct
     4621 gatcgaagcg ctttccaaat ccatcgttga aatttcctcc tcgagcctta cgacgatctc
     4681 cggtcttgtt gcgctgatgc tcatgcagtt ccggctgggc tatgacctcg gtctggtgct
     4741 ttccaagggc atactttgca gccttatcac ggtatttctt ctgatgccgg gactgatcgc
     4801 ctcgtttttc cgcccgctgc gcagaacgac gcacaagtcc catgtgccgg atatcaccgg
     4861 ctggggacgg ttcctcatga aaacacgctt cggctttgtc gcggtgtttg ttattatcct
     4921 tccgcttgcc atctggtgct cgagccggac ggaatacgcg nnnnnnnnnn gaatacgcgt
     4981 tcaacgacgc gggcatcgac gaactcaaat actccgagag ccgcgcggcg gcgcggaaga
     5041 tcaccggcac ctttgcgaac gacacgacga tcgccgtgct cgttccgagc ggaaactacg
     5101 aagcggaaaa gcagttcctg cgcgacgccg cggagctcga caatgtgaag accgtcaccg
     5161 ggcttgcgaa tatcgagatc gaggacggga aagtgttgac ggattcctat acgccgcgca
     5221 tgttctccat gctgctgaac accgattttg aagaagcgga aatgctctat gccgcctacg
     5281 gcgtggagaa cgggcagtat cagccgatct tcggcaatgc cgagacctat tccgtgccgc
     5341 tcatcgatat gtttcttttc ctctttgaaa agatcgatca gggcatcgtg acactcgacg
     5401 ccgattcgca ggagacgctg gacagcctcc gcggtgagct ggaacgcggc gaagcgcagc
     5461 ttcgcggcga aaactgggac cgtatggtca tcacggcgga cgtacccatc gagggtgacg
     5521 agagcgtcgc gctcgtaaac gcactgcgct cgctggccga cggacgctac ggagaaggag
     5581 ggtgcctcgt catcggcgac gtgacgagcg ccaaggagct ggcagacacc ttcaacggcg
     5641 attcgctgct catcaatctg ctcaccatcg cctttgtttt catcatcctg atttttacct
     5701 tccgctccgt tgtgggtgcg gcgctcctcg tgttcgtcat tcagggcagt atctggatca
     5761 actttacatt tccgtacctg acacataccc gcgcgtcatt cgtcacgaat atgatcgtct
     5821 ccgccattca gatgggtgcg acgatcgact atgccattgt tatcatgagc cggtatctgg
     5881 atctcaaaaa gctccatacg ccgcaggagg cgatggcgca ggcggtcaac gagagctttc
     5941 cgaccgttat gacctccggc accatcatga ccgttgcagg cattctgatc gcataccgcg
     6001 tgtcggacgt atacatcggc cacatcggtc tcgccgttgg ccgcggcgcg cttatatcgg
     6061 tcatcctcgt tttgagcgtt ctgccgcagc tcatcgtgct gctcgacaag gtcattgaaa
     6121 aaacaacatt tcggaaaaaa acgacgacgg gaggtgccgt ggaatgaaac gatggatcgc
     6181 agcgctgctg agcacggtgc tgcttctttc cgtgtccggc gcggcgctcg ccgccgaccc
     6241 ggataccgtt gagatatcct ctgccgagga tctcgcggag ctttccgcgc tgtgcacgtc
     6301 ggacgcatgg tcggaaggga agacgatcgt gctcacgcgc gacatcagtc tcacaggctg
     6361 cgatttttcg cccatcccgc tgctggccgg aacgtttgac ggccgcggcc acagcatact
     6421 cggcatatcc cttgacggcg acgcctcaac acagggactt ttccgtatgg tgctgaaaac
     6481 cggcgtcgtg aaaaatctca cggtctccgg cacgctccac gcaaccggaa acggcgaaaa
     6541 catcggcgct atcgccgggg tgaactacgg agtgatcgaa aattgccgct ttgacgggga
     6601 tattctcgcg caggctgcgg cgggcggcat tgtcgggctg aaccaagagg gcgctctcgt
     6661 gagcgagtgt aaaacttccg gctccatttc ggcctaccac cgcgccggcg gcatcgcggg
     6721 cgagaaccgc ggcgtgctca gcgagtgcga aaaccgcatg agcgttaaca cggattatat
     6781 cgcagtcgag aagcccgacc agaaaaaatc gtttgatatt tccacgctta ccctgaacga
     6841 ggaaaccatc attgatatca cggatctcgg cggtatcgcc gggctgaata cttccgttat
     6901 caagtactgc gacaactacg gcgacgtggg atacccccac acgggttata acgtcggcgg
     6961 cattgccgga cgccagagcg gccgcgttct tggctgcaca aacgccggag aggtcactgc
     7021 acgcaaggac gtcggcggca tcgtcgggca ggtggagccg tatacgtcgt ggaacgttac
     7081 cggcacaggt ctcggcgaag tgcagtccca gctttaccgg ctggagagtc tgctgcgcac
     7141 tacgctcggc gatttcggcg attcacaggc cgaagcgcgt gcgctgatgc agcaaatcct
     7201 tgagctgctc ggaaactgca gcgatatcat cggcggaatg tatcccgaca ttccatggcc
     7261 gacacccggc ggcgacggcg gcagcgatac cggagatgcc ggcgggggga gcgacaacgg
     7321 aaacgccggc agcggcgatg acaccaccgg cggatggatc gatccgggca gcggctcgat
     7381 ggacgatctc tccgataatc ttcagcagat cgtacgtctt ctcggccaga tgacggacgt
     7441 ctttacgagc gacgccgtga tcgaggatat gcagaacgtc ctctcccagc tcatgaacgt
     7501 ctcttcggcg atcatgagca tggcgtacag tctcggcaat gcctcggttc agcttgagga
     7561 catttccgac accgacgatg acgatgacga tgaagcgctc tgcctcatcg cgcagtgcgc
     7621 caatacggcg tccatcgctg ccgatacgaa tgtcggcggc atcgcgggaa acgtgtcgct
     7681 cgatatctcc tttgaccgag aggatcaact caacatttcc tctacgctga tcggcagcgg
     7741 gaaatatgag atttttgcgc gcatttcgtc ctgcgaaaac agcgcttccg tcgcggcctc
     7801 caagtcctgt gccggcggta tcgccggacg aatggattac ggtctcgcgg tcggctgctc
     7861 cgcgctcggc gaggtgacaa cggcggagga gtatgccggt ggcatcgtcg gacacagctc
     7921 tgccgccgtg aggaattgcc gcgcgcgcgt caatctctcg ggcaaacggt atgtcggcgg
     7981 cattgcnnnn nnnnnncttt ggcagcgtca cgagcgattc cggcgactat gtcggcggca
     8041 ttgccgggct cggcaaggac atctcctcgt gcagcgtcat gccgcacttt gagaatcgcg
     8101 cggagctctg cggctccgtg gcgggctatg ccgacggtac gatcgccgaa aatctctatt
     8161 ccgactccac ggtcggcggt gtggacggct tcagcttcac cggtcagtcg gactatatgg
     8221 actatgagga ttttgcggcc cttccggaca cgccggactt tttccgcagc atcggtgtga
     8281 cgtttgtaaa ggacggcgtg acggtggaga cggtcgaagt gcccttcggc ggcaggatcg
     8341 cctcggtccc ctcggtcgcg gacgaggacg gaatgtactg gcagtggaac gactttgatc
     8401 ccaacgaggc cgtatactac agcaggaccg tcgaggggga gtatatccgg ccggtcacaa
     8461 cgatctcgac cggcgaggac gagccgctgt tccttgcgga gggcactttc cgcgacgggc
     8521 agacgcttct tgctgtgccc tttgtgccgg atgcggaaac gctcggcatc gatgcggcgt
     8581 ccattctcgc ggcatacacg gtgcgcgttt ccggctattc cgagcccctc accgtgcgca
     8641 tgcttacctc ggcgtccggg agtctctata cactctcgga aggcacgctc acgccgcttt
     8701 cgttcacccg agacggcagc tatattgttt tccggctgga caacggcgca tcgatcgtgt
     8761 accttgcgca ggaaacctcc cgtaccggct ggatcattgg cggcattgcg ggcggcgctg
     8821 cagccgccgc ggcggtcgtc ctgattatcg tgcgaaaaag aagaaaaaag acttaacaag
     8881 ttcttgacga attcttgatt tccctgtatg ctttatatgt attctctccg gcggtacgtc
     8941 cgcacaatcc gccggggaga cgtattttgt aaagcagaaa ggtgaaatga gagtggaatt
     9001 acttcattgg gcggagagtg tattaaagta ccttgtgcag ttcgggatca ttcttctgga
     9061 gggaacggga gcggtcattc tcctgttttc cggcgcgaag agcgttgtcc gactttgtaa
     9121 aaatcagccg catgcacggc tctccatggc gcagggcatt gcgctcgcgc tgtgcttcaa
     9181 gctcggcggc gaggtgctgc gcacggtgat cgtgcgcgag tggagcgaac ttctcgtgct
     9241 cggtgcgatc attttcctcc acgcggctat gagcatcctc atccattggg agatcaagat
     9301 ggaggagaag cgcctgtccg ccatgagcga ccttgccgac tgatacatca aattaaagaa
     9361 gcttcgtggt tataaaaatc acgaagcttt tttgtagctt tatttttgtg atgcacaaaa
     9421 gcaggtcttt gtccgaacag gctcttgaaa ttctccgcat tgtcaggtag aattattgtg
     9481 tataagcact aaaaggaatc gaggcgatca atcagaacaa caaaggaggc ttttacacat
     9541 gaagaaaagg aaaatcgtat caacgctcct tgcgctgctg cttctcgcca gcctgcccgt
     9601 gtcggcgctg gcggcggagt gggacatcag caagggcgac atcaccgtaa acgccggaag
     9661 cggcgggcag acggtcaccc aggggtcgca ggtcgatgtt ccggacagcg cccccgtcat
     9721 tacgggaagc tccacggaaa acaccctcgc catcaacgcg gagaaggatc agaccgcaaa
     9781 tgtcaccctc tccgatgtga acatcgacgt gagcagtgag ggcaaggccg ccgttaccac
     9841 caccggcgag ggcaatgtca gcatcgagct gaacggcgac aacacgctca agagcggtaa
     9901 gggtcatgcc ggccttgaaa agaaaaacgg cggcaagctg accattgcgg acgaggacaa
     9961 aaacggcaag ctgaccgccg agggcggcaa atacggcgcg ggcataggcg gcggcgatca
    10021 gggagctggc agcggcatca ccatcaccgg cggcgagatc aaggccaccg gcggccaata
    10081 cggtgccggc atcggcggcg gcaagggaga tggcagcgac atcaccatct ccggcggcga
    10141 ggtcaatgcc agcggcgg
//
LOCUS       DAWAAE010000071        10043 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3020_length_10043_cov_3.065178, whole genome shotgun sequence.
ACCESSION   DAWAAE010000071 DAWAAE010000000
VERSION     DAWAAE010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3020_length_10043_cov_3.065178"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4629..4638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1969 a         2988 c         2904 g         2172 t
ORIGIN      
        1 tcttccggat caggacgcga agccctacgg ccggagaggg gtgctctccc gcgcgcgctt
       61 ccggcgcgag atggcggaca cggtgcggct tctttataac tgcccgtgca tcgcgctctg
      121 gacggtgttc aacgagggct ggggtcagtt tgacgcgctg gacatggcaa acgagctcaa
      181 aaagctcgat cccacgcgct ttatcgacca cgcgagcggc tggcaggatc agggctatga
      241 ggaaatgaag agccgccaca tctatttccg tcccgtgaag ctcaaaaacg atgggcgcgc
      301 gcttgcgctc acggagtacg gcggatacat tctcgccgtg gacggccatc gctggacgga
      361 caaggtgttc ggctaccgca tctgccgcga cagcgcggcg ctcgcgcacg actacgaaaa
      421 gctcatgctc gggcaggtgc tgccgcacat ccgaaaggaa gggctgaccg cggcggtata
      481 tacgcagatc accgacgtgg aggaagagct caacggtctc atgacctacg accgcgaggt
      541 gctgaaaatt ccggaggaga cactccggga gatcaatgcc gcgctgcggt ttgatacata
      601 atacggcttt ggagggcagc actggaatga acgaaatcaa aacggaaaag aaaaacctct
      661 atctgcgatt ttcggactgg atggacggcg cgctctggca ccgggcgctt gtccttctcg
      721 ccctctcggc tgccgtgata acggttttct ccgtatacac cacgccgatc agcccctatt
      781 acggcaatga cagcgcgttt ttcctcctga tgggaaaagg tgttacacag gggaagatcc
      841 cctatctgga tctgtatgac cagaaaggtc cgatgatctt ctatgtgaat gcccttggct
      901 atctgctcac cggcgaccgg tacggcattt tcctcatgca gatcgtcaat ctgacggcgg
      961 cgtgcctgat cgtataccgg cttgcgcggt tctggctgcg cagcggcgct tcgctcgccg
     1021 tggtcgcggc gtatcttttc gtctatgtcg gcaccgtgca ggacgggaac atgacggagg
     1081 aatggtcgca gctcttcttg ctgctgccga tgtatctctc gctgcggttt ctcaaaagcg
     1141 gcgcgagcgt gagcgagcat cccaaatggt acagtttcgt atacggcgta tgcttcggcg
     1201 tgctgttgat gttccgcgtc acgaatgccg cgccggtcgg cgggctgatc ctcgcataca
     1261 tcatcctgtt ctgccgggaa aagaaggtcg ggcagctttt ccttcatgcg gcgatcgttc
     1321 tgttcggcac ggcgctcgtc gttgttccgt tctgtctgta ttttctgcgg gtgggcgcgt
     1381 ttgatctgtt catttacgcg agcatcacgc acaacttctt ctacgccacc ggcggagcga
     1441 ccgcaagaga cgccgtcggc tggctctcca tcatcggaag cgttcttttc gttccgtgct
     1501 ttttcgccgc ttaccggccg ctcacgcgct gcggtacgct cgacgggcgg acgtatacgc
     1561 tcatcggctg ctatgccgcc gtcggcgcgg tcacgatggc gttcggcttc acctaccgtc
     1621 attattttct caatctcgcg cctgcggtcg ttgtcattct cgccgtcggt atcgactggg
     1681 tgcagcgtac cgcgccggag aaccggcgca aagcccgcag tcttctcgcc gcggcgctgc
     1741 tgctgtgcgt cgttcccttc gccccgcagg tcgtccggca gtgcggcaag gtggtctatt
     1801 tcacctgtat gcacaagctc gaccgtttcg ccagcaaccg attggagctt gccgacgcct
     1861 ttcccacaga ccgcgacagc gtgtggggtt acgatgtgct cgcgcaggac tatctctatg
     1921 ccgatatcat cccctgcttc cggtactttg ccatccagcg atggatggag gattccgatc
     1981 cgcagatcgc gcccgagatc gacgaaatgc tggcgaccga tccgcccgtc tgggtcttca
     2041 tctatcagga agataccgaa atggcagaga agcttctctc gctcggctat aaggcagagc
     2101 cgcgttcggt gtttctgatc ttccacagag ctgcataaaa accgtccgcc ggtgcttttc
     2161 aggcatcggc ggacggtttt tcgtatgcgg cagagccgct cttttcttta ttctttcttt
     2221 tcttcgtcac ggaagagata tacgcgggtc tcatacggct gcgtgacaaa gccgttgttc
     2281 tcgatatagt ccgtgtggct cttcaaaagg agctttccct tccgcagatc gtacccgcgg
     2341 ggtgccttga acttcacggg attcaccgag tacgagcaga tgacgagaag ccgttcgcgc
     2401 ttggcccggc gttcgtacac aaagagcctg ttgctgagcg gatagtactg acggaacgtg
     2461 ccgtagcgca cgacaggcag ctcgtgccgg agacggagcg cctcgcggta gaagttcagc
     2521 agcgagtcgg ggtcatcctc ctgcgcggca acgttgatct tcgggtagtt ctcgttgacg
     2581 gcaaaccacg gcgtgccggt cgtaaacccg gcgttcggcg cgtcgctcca ctgcacgggc
     2641 gtgcgcgcgc tgtcgcgcga gccccagtag atacgctgca tgcgcttctt ttcggatttg
     2701 tgcgtggcga agtggtggta ctggttgatg gtctgcacgt cctcgtagcc gtcgatcgtc
     2761 tcgggataga tgttcgtcat gccgatctcc tgtccctgat agacgatggg cgtgccctcc
     2821 tggaagagat acgacgccgc gagcgccttt ccgctctcgg tgttgtactt ttcgctgccg
     2881 tagcggctga tgacgcgggg atggtcgtgg ttttcaatat acagaagatt ccagcccttc
     2941 ccgcgcagcg ccttctgcca gtgctgccag atggtcttga gctttacgag gcagaacggg
     3001 tgatgggaat agtcctgcag gaagcagtcg ccctccatgc actggaacgg gatcatcatg
     3061 tcaagctcgg ggtgcttgcc gccgatgaag tcgagtgcgc gcttcgtcgt gatgagcggg
     3121 gcctcgccga tggtcacgca gtcgtaatcg tcgagcacgg tgcggaactc gttgagatat
     3181 tcgtgcaggt gcgggccgtt gttatactgg aaaatgccgc gcgccatggg catataaaac
     3241 gacgcgtccg gcagtccctt gggcttggag atgtaggtga tgacatcctc gcggaagccg
     3301 tccacgccca tatccagcca gaagcgcagg attttcttta cctcggcgcg gacgcggggg
     3361 ttgtccatat tcaggtcggg ctgcttgatg gcaaaaatat ggagatagta cgccttgcgc
     3421 acctcgtcgt actgccacgc cttgccctcg aacatgctgt cccagttgtt ggggagcgtg
     3481 ccgtcctttt tcggcttgcg ccagatgtaa taatccttat acggctcttc cccgcggcgg
     3541 ctcgcctgga accacgggtg ctcgtcgctc gtgtggttta cgacaagatc catgatgacc
     3601 ctgatgccgc gctcgtgcgc gccgtcgagt acctttttga acgccgccat gccgccgtat
     3661 tcggcggaga tgtccatata gtccgccacg tcgtagccaa agtcggcgtt cggcgagggg
     3721 tagagcggcg aaaaccagat ggcgtccaca tgcaggctct taagatagtc cagcttttca
     3781 tacacgccca tcagatcgcc cacgccgtcg ccgtcagcgt ccttgaagct gcgcggccag
     3841 atctggtaga tgaccgcgtc tttataccag aaagtttttt ccatgtctat gccctttccg
     3901 ccgctcaccg cagcttttcc tcgcagaaaa tgcgcatatt ttcgatccgg acggtctccc
     3961 cgtccggcag ctcgaccgtg ccggagagcg ttccgaaata ctgccgcgtc tcggagtgca
     4021 gcagcatcgc gcgccgccgg acgcggtatt cgcgccgcac cgcaaattcc agatgcagcc
     4081 gccgggtgct gtcggaaacg taccacggtc gcagcgggtc ggacgcgctg cgcttcatgt
     4141 atacgcgccc gagcttgcag agcgtgccgt cataaaagag cgcgttttcg tccgcgctgt
     4201 ccacgccgtc ggagagggca agggagatca catgcccgtc gacgcacgcg ctgccgaacg
     4261 cgcggagata cacatcgccg cgcggcatca cggcgcgcac gctctcggag aagagaaacg
     4321 tctgcgcgtc gagcggcaga tcgcggttgt gcatccgaag atacccggaa agaccgggga
     4381 agcaccggaa gccccggtaa taaaactgct ttccggcaag cgcctcggcg gtataaatgc
     4441 cgtcgccgtc gttcgcgcag gagagatcgc cctcgaaccg ctccgagcgg aactgaatgc
     4501 ggcgtacgcc gccgtaatgc gtgagcagga gatacagatc gtcgttttca aacttgacgg
     4561 tgtgttcctc gccgcaggag aaatccagat cgaacgcatc gcccggaaac gcctgcacgg
     4621 tgccggagnn nnnnnnnngt gccggaggca taccgctcgt ccgtttcgag atcggtgagg
     4681 cgcacctcgg caatgccgcc ggagcgcgta tgcccgtagc tgatctccag cgcgaaggac
     4741 gccccggtga caaaatagcg ttcccattcc gagagaccgc cgccggcggc ttccttgtta
     4801 tagataaaat cgtcctgtgc cgaccagccg ggacgggcga gctttccctt ttcgttcagt
     4861 agctccgccg gtgcgcggta ttcttccatg tcagacctcc atgatatgca gcgcggcgat
     4921 ctccgggtct tggatgcgcc gcatgacggc gtaagcgtgg ggctcgagga aatcccagcc
     4981 gccgtccgtc aggccctgcg cgcgcagctc ttcgatcact gcggcgctca cgcgctcgat
     5041 cgtgtcctcg tccggctcgc gcagcagcgc ttcgagcgtc gggatgagtt ccgagagctt
     5101 cggcagctct ttcgcggcgc gaaacgccca tttataaaac ggcatatgcc gctcattcag
     5161 caggaaaagc atggcgagcg tatggtttac aaactcgcag cacgcgagcc gcgccgcggc
     5221 ctcttccccg tggcggcggc agcgggagac gttgtactgc ccggactgcg ccatgagcac
     5281 ggcgtgtccc gcgatctttt tgactcgcac gtcctccggc atgccggtgc gcagcgtttc
     5341 gcggatgccc gtcatgtcgc cggagccatc gtagaatatc tccccggcgc acgcggcggc
     5401 gagagcatag gacgggatcg ccatccattc gcgccacgtt tccggcgtat ggaaaaggcc
     5461 cgtctgctcc agaaggaact gctcggtcgt cttaacgccg tatcgcccgt ttccctgacg
     5521 gctgcggtgc tcggtcttca cgccctgaaa ctcccgcggc aggcggtcgt agagcgcgga
     5581 gagaaggaag ccgtgcgttt cggcgtcctc cggcgcgaga aagagcatgc accccgcggc
     5641 gtagccgtgg tcgtgggaga gctcgtcatc gtaccctaag cagtccgaac cgccgccgca
     5701 gagccccgca gcaagccgcg gcagcagcac ggggcaggtt tcccggatcg ccgggcgcac
     5761 ggcgtcctcc cagaaggcgc gggagagctc aagtcccttc atagatgata tccgcccttt
     5821 cccggagatc gcgcgccgcg tccgcgcgcc caagcgcttc gagcgccggg gcgatcttcc
     5881 gcgccgtgtg cgcgtagtag tattcccaga cggcgtccgg gctgtccatg cgctccatcg
     5941 ccttatcgag acacgcggca atacgcgcat cgttctcatc ctgcgcggca taatgctgcg
     6001 caagattcac atacgtcacc gcgagatcga gatcgccggg acgctttttc tggttttcca
     6061 gcgcgcggag catgtaccgc tccgcctcgt ccgggcgatg cagcgcatcg agcgccgcgg
     6121 ccatgttgtt ataaagcgcc gcaaggcgcg gatgatctgc gccgagcgaa acggcataca
     6181 ggctctccgc ctcccggaag agcggcagag cgcgctgtgc ctcgccgaag gcggcgagcg
     6241 ccgtggccgc gtttataagg atcgtcccgc gcgcggcagc gccggggcgc acctcactga
     6301 gcagctccgt gcaccggccg agcaccgggt aaaatttctc cgcatcagcg cgctggcggt
     6361 aaaagcccat cagctcgctg cacagggaaa gcgcggtgcg cctgtcgccg gtctcctccg
     6421 cttcggtgag cgcggcaagg aatatgcgct ccgcgccgtc ccagtcgccg cggcccatgc
     6481 agtcgtccgc tctcgcggct atactgtcca ttgtttcttt catacggcga ttataccttg
     6541 aattcccggc ggttgcaagg tataatacgt atgttcatca tttcacgaaa agaggtttct
     6601 acctatgaaa aagactgtta tgcgctccta cgcgaagctc atcgtgcgcg tgggtgcgaa
     6661 cgtccagaag ggtcaggagg tgcgcgtttt cgcgtctctc gaccagccgg agttcatcaa
     6721 gatgctcgcc gaagagtgct acaaggccgg cgcgagccgc gtaaccgttg actggaatta
     6781 ccccgagctt acgaagctcg ccgcgcgcta catgaagctg cgcgacctct ccgagacccg
     6841 cgagtgggaa aaggcgcgta tgcaggacat ggtggaccat ctgccggtgc gcatcttcat
     6901 cgagtccgaa gatcccgacg gactgcgcgg catcaacccg aagtacttca aggcgtttgc
     6961 cgcgcggatc aaaatctcca agccctaccg cgacgccatc gacaacaagc accagtggtg
     7021 catcgccgcc gtgccgggtg aggcgtgggc gaagaaggtc caccccgaac tcagcaagcg
     7081 cgccgccgtg gagcagctct ggaaggacat tctctacacc gcccgcgccg acggcgagga
     7141 ccccatcgcc gattgggaag agcacaaccg cgatctcaag gcgcgcagca aatatctgaa
     7201 cgatctccat ctccgcgagc tgcgctacca ttccgcgaac ggcacggatt tcaaggtcgg
     7261 cctcatcccg acggccgagt tccacgccgg acgcgatacg acgatgcagg gcgttgtcta
     7321 cgatcccaac atgccgaccg aggaggtctt cacctctccc gaccgccgca ctgccgaggg
     7381 catcgtttac gccacgaagc cgctctccta tcagggccag ctcatcgaga atttctccgt
     7441 ccgctttgaa aagggccgcg ccgtggaggt caaggccgaa aagggacagg atgttctcga
     7501 gcagatcatc agcatggacg agggctgcca ctatcttggc gagtgcgcgc tcgtgccgaa
     7561 ggagtccccg atccatcagt ccggcctgct cttctacaac acgctctttg acgagaacgc
     7621 cgcctgccat ctggcgctcg gcttcggctt tgacgagtgc gtaaagggct ttgagaagat
     7681 gaccaaggaa gagctctacg agatcggcgt caatgacgcc ggcaaccaca ccgactttat
     7741 gatcggcagc gacgatctct ccatcgacgg cgtggacgag cacggcaacg tgcatcccat
     7801 cttccgcaac ggcacctggg cgttctaaaa tgcctccctc tttgagggag gtggctcgcc
     7861 gaaggcgaga cggaaggagg ggctttttct atgtccctca attattccgg cgatttgatt
     7921 cttagagcaa aagagctgcg aaaaactgcg accccgcagg aacggcattt atggtatgac
     7981 tttctttccc gttatccggt gcggtttcag cggcaaaaaa ccgtaggccg atatattgtt
     8041 gacttctatt gccacagagc acgtcttgtc gtagagttag acggcagcca gcattatgag
     8101 gagcaaggca tcgccagaga tgcagaacga acgacgtatt tgatgcagca agggcttttt
     8161 gtcctgcgtt tttccaatgc cgagatcaat tgcaagtttc attttgtctg tgagcaaatc
     8221 gaccgaacag ttaaagaacg gctcgcattt taattacctc cccctttgag ggaggtggct
     8281 cggcgttagc caagacggaa ggagttcact ccctcagtca gctgacgctg acagctccct
     8341 ctgagaggga gccaatacgt ccatccaaaa accggcagaa gatctgccgg tttttgcaat
     8401 ttctgaaacg atatggtatt atacaacaca agtcagaaaa gaatagcggc tgtctcaagc
     8461 ccgtgaaggg tatcggtatg gagccgctgg gaggttcttg tatggtacag tttcagaagg
     8521 acgccgcgct cggcggcatc ccgttcggcg tgctggaggt cgtttatccg gacaaggcgc
     8581 tctggcgcac ggaggacttc cgcataatgg tcgaggagca tctgcgtctg ctgcgggaga
     8641 aatacgccga ctatgaccgc aaggcgcttt ttggcgaaaa cgtctatttc cggtttttca
     8701 ggaaattcaa aaagacatac ccggtcatga tgcagcttga atccttcctc ctgaaaggcc
     8761 gcccgttccc gatcgacaac agcgtgaccg ccatcccgtt tctcgcggag ctcgaaacgc
     8821 agatcctctc cggcacgcac gacgtggact ttgtccgcgg ggctgtccgc ctgtttgccg
     8881 gaacggaaaa agcgccgttt cccggcatgc ggggtgagga ggtacatacc taccccggcg
     8941 acttctgcgc gcgggacgat gagggcatta ttttcagcat gatcgccgga gcggacgcac
     9001 gcacctgcgt caaggagacg agccggcatg tgttctaccc cgtgttcggc gtggccggac
     9061 agccggaggc ggagatatcc gccgtgctcg accggctgga agaatacgtc cgtgttctcg
     9121 cgtcggaggc agagaccgcg cggcagcttc tgggaaaata aaaagatgag ctgacaccgt
     9181 acagcgcgcc gttttatcca ataatacgca aagcagcacc accccgcggg gtggtgctgc
     9241 tttgccaatt atgcgggttt tctcttttat tcgctataaa gctcaatact ggtagaatga
     9301 ccatttacgg catatatagc cacagttccg ataatagcgg caaaaatatt gcccgttacc
     9361 attacagtat cgccggggtt taggttaagt acatctgctg cgctttcagc ttccacttcc
     9421 cagccgcctt ccaattcaac atatgcttcc atcgaatacc cattcacagt gcaagacccg
     9481 tgaacagctt taaccgtgct tattattctg attggcgcgc caaagtacaa tgctttaaat
     9541 tttacaccgt tgcttttttg aatatcattt aactccttcg cagtaaggtt ttctatttca
     9601 ccttcattat ttacaatctc aatcaatgtt tggtattttg tactctcagc aaggctctca
     9661 tattgactga ctggaatata cgtcatctcc ccgtcttctc ccttgagtaa tatcccgtct
     9721 tcttccgttc tgtctaatgt gaaatgagct tttatataaa cattgtcatc ttctatttcc
     9781 catgtaatag gaatatccat tgaggcagcc cccgcgctca gcgttccggt gccatcgtca
     9841 aacagtgaga ggctttctga cccatctgat tctcgccaag gctctttgat aaggatgtcc
     9901 atattctctt tctccgcgtc agatttcatc tgaggctcag cgctattgct tccgcatgca
     9961 cataaagaaa tgcatacggt caatgtcaaa aacattgcaa caaaccgttt cattttttat
    10021 cctacctttg taaatattag cac
//
LOCUS       DAWAAE010000072         9711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3152_length_9711_cov_2.652030, whole genome shotgun sequence.
ACCESSION   DAWAAE010000072 DAWAAE010000000
VERSION     DAWAAE010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_9711_cov_2.652030"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2056 a         2744 c         2997 g         1914 t
ORIGIN      
        1 acaccaacgc cttcagcgtt gacgccgtgg aggagatcct ctcctggggg atcaaaaacg
       61 ggtattcctt ccgcgcgctg gatctcacca gcccggcggt gcatcattct gtagccaatt
      121 aggaggcagg agaacatgac cctttttgtt gcctttgaga tcctcggcac gatcgccttt
      181 gccgtttccg gcgcggtcgt tgccatatcg aagaagatgg acattttcgg cgtggcgatc
      241 ctcggcatga cgacagcggt cggcggcggt atactgcgcg atctcattct cggtatcacg
      301 ccgcccgcgg cgttccagtc gccggggtac gcgctcacgt cgatcgccgt ttccatgctt
      361 gttttcctgc ccccggtgcg gcggctgctg cactggaacg agaagcttta cgacacggcg
      421 cttctgctct ttgacgccat cggtctcggc atattcaccg tgatcggcgt gcagacagcg
      481 tacatagcta cgggcgagct gaacgcgttc cttgccattt tcgtcggcgt tgtcaccggc
      541 tgcggcggcg gcgtgatgcg cgatgtgttc gccggatcac ctccgtatat ttttgtcaag
      601 catttttacg cgaccgcgtc cctcctcggc gccgtggcgt gcctcctgct ctggaacatc
      661 gggcagatgc ccgccatgct catcggcgcc gccgttacgc tcacgctgcg cctgcttgcg
      721 gcgcattacc gttggagcct gccgaaggcg aaataagata gaggggagaa ggctccatgg
      781 tactgcatga actgctcggc ctgccggagc gggagaagca cgtcgtttct ctctgcggcg
      841 gcggcggaaa gacgacactg atgtacgcgc ttgcgcgtga aacgaaagcc attgtgccga
      901 cggcgctttt taccacaacg cacatcatga cgccgtctga tgcggacgtt gttctctccg
      961 tcccgttttc ggcggaggaa tgccgcgcgg cgtggcgcga agggaagatc gcctcgtccg
     1021 ggacatttct ttcggaagag cggaaattcg gcgcgccggg ggaggatgcg atgcgctttc
     1081 tctgccgcga ggcgtccgcc gttatcattg aggcagacgg cgcgcgccgt ctgccagtga
     1141 aattcccggc ggcgcgggag ccggtgatcc gcccggaaac gacgcatacc atcgttgtgg
     1201 cagggctctc cgcgctcgga aaaaagccgg aggagatcgt ccaccggtac gcgctggcaa
     1261 agaacaccgt ggatctttcc ggggattccc tcaccgaaaa acagatggcg gagcttctct
     1321 gggcagggta cggacgtttt gcaccgatct tctttatcaa tcaggcggac accagcgatc
     1381 ttgcgcagcg cggcgagcat atctgcgcca tcctccgtga tttcggcgcg cgccgcgcag
     1441 tcacagcctc gctccacgca cttagaaaat cgtacgaaag gggatgaccg gcatgaaacc
     1501 gatgaaacga ttctactacc gggcctacca gaccgtattt gacctcggtt cgcgcgtttt
     1561 accctggcgc aagccggaga tcgtcagcgg cgcgggcagc acggcgagga tcccggagct
     1621 gctgcgcagc tgcggcgtga aaaaggtgat gatcgtctcc ggcaaacata cgggcgcggc
     1681 gctcgtcccg ccgatccgcg cgcagatcga agcggcaggc atttccgccg tccactttga
     1741 cgccgtgcgc gccaatccca cgatctccat catcgagcag gtgcgggaac ggtatctcgc
     1801 cgagggctgc gacggcttcc ttgccgtggg cggcggctcg ccgatggaca cggccaaggc
     1861 tgccgcggcg cgcatcgtgc gccccaaccg taccgtggag cagatgacgg gacttttcaa
     1921 ggtcatgcac gctctcccgc cgttcatcgc tgtgccgaca acggccggtt ccggctcgga
     1981 gacgacgatc gccgccgtta tcaccaacga aaaaacgcac cacaagcacg ccattatgga
     2041 tctctgcctc gtgccgcgct tcgctgtgct cgacgccgtg ctcaccgagg accttcctcc
     2101 ggcgacaacg gcacagaccg gcatggacgc gctcacgcac gcggtggagg catacgtcag
     2161 cgtccacagc acaacgaagg agaccgaccg ctgcgcggaa gaggcgacag cggcgatctt
     2221 ccggtatctg gaacgggcat atgtgaacgg acacgatctt gaagcgcggc aggaaatgct
     2281 gcttgcttcg ttcaaggcag gcgtcgcctt cacccgcgcg ggcgtcggaa acgtccacgc
     2341 gattgcccat acgctcggcg ggctgtataa tacggcgcac ggactggcaa acgctgtcat
     2401 tctccccatc gttttggagg attacggcac agcggcggag gaaaagctcg cccgccttgc
     2461 cgagatcacc ggaacgaaaa cgaccggcac cgcgcatgag aagagcgcgg cgttcatcgc
     2521 cgcaatccgc gccatgaacg tgcgcatggg cattcccggg catctggacg tgatccgcaa
     2581 ggacgatatc ccgcagatgg tcgcctgggc gctgcgcgag gcaaacccga catatcccgt
     2641 tccggttttg tacgacgagg cgcactgcgc cgaggtcatc cgccgggttc gcggataaga
     2701 tgacgaaaag cgccggaacg aacaaagttc cggcgctttc gcgcataata aaaggttact
     2761 ggagcaggct ccggtagctg tcgcacgtcg agagattgtc gggcggataa aattcctcca
     2821 ccgggaagcg gatgcgcccg aagagcgtgc acggatcgtc gtccgtcgtg ccgacgcact
     2881 cgttttccgg gaagcagtag ttgtagaccg ggatgacgag ctggctgtcg cgctcgagcc
     2941 ggatgagaga aaactggccg agcgtcgcgt accagagccg gttggagtcg gtgagcacaa
     3001 gctcctccgg aaacgcggcg agaatgttgg ccggcacctc cgggatgcgg tcgagcggcg
     3061 tggcgcagtc cttctcagtg atctgcatcg aaagcgcgat cggatcgacc gcctccacaa
     3121 cgccgacggg ctgatcggcc tgcgtggtga tctcgccgtc agaggtgtag accttgacgc
     3181 tcccctcgct gccgaagaga atgacgcgtt tattgaatac acagagaccg gtgacggttt
     3241 ttgcgttcgg gacgcattcg ccggtgacac ggtaaaagta ggtgacgtct accgtatagt
     3301 acccgcggtt gaacgtgatc tcctcgacat ttaccccggc atagagcagc tcggcgcttt
     3361 tcgggcggat gctgaaggcg ttttctatgt agccctgcga cgtccgcgtc ggaaacaccc
     3421 gcagatcctc tacgcagtcc ttatcccggc agctggagta tattttcttc gtatggatac
     3481 aaacagcctc gcgaacgcat gcgctgctgt ccaccggacc gggtacgacc ctgtctgcca
     3541 tatcggaatt cctcctttaa caaagttcag tacagtctat gcgcgcgccg ccccaatgtg
     3601 ccgattgacg ccctttggaa aaagtgataa acttgctata tatataaata tgaggagaga
     3661 ttccgatgga aggatatcag aactttcagg aaaagctgga tataaagatt ctcattctgt
     3721 tcattctgcg ccgtctgccg gatgccgtga tgcgggaaac gctcacggat ctcgcccttt
     3781 ccgagggcaa cgcggggtat tttgaatact ccgagtgtct cgcggagctg gtcgattccc
     3841 atcaggtgga gcaggaagga aacctctaca agatttccga gcgcggcgcg cgaaactgcg
     3901 agatcgtgga gagcagtctg ccgatgacgg tccgccagaa aatggaaaaa aatataaaac
     3961 cggtcgcgga tgagctgcgc cgccgcgcga tgatcaccgc ctcgcacgaa acggcggaca
     4021 acgtctgccg cgtacatctt gctctttcgg acggcatggg cgagatattg aacctgcaaa
     4081 tgctctgcgg cggggaagag caggcggaga agctggaaga gaatttccgg aaaaacgcgg
     4141 agaaatacta tacgcagatc atggagctcc tgtcgcgtga tgaaaaagta taagattttt
     4201 gttatcaatc ccggctcgac ctcgacgaag ctggcgcttt tcgagaacga acataaaatt
     4261 ctggaaacca atgtgtttca cgattcctcg gtgctgctgc aattcccgac ggtaaacgcg
     4321 cagctcccgt accgcaaaaa gctcatcatg gactttctga gcgagaacca tatcgacctt
     4381 tcggacgtgg acgccatcgt cggccgcggc ggcagctgtt acccggtggc gagcggcgta
     4441 tacgttatcg atgataaact catcgaggac accgccgccg ggaagggcgg catggagcat
     4501 ccgtccaatc tcggcgtgca gcttgcgcgc gagctgcaga aggagttcgg cggccggatc
     4561 ttcatggtcg atccgccgtg cgtagacgag ctgtgcgata tggcgcgcat gaccggcatc
     4621 gacggcgtgc cgcgccacac caatatgcac gcgctcaacc ttcgcggcgt tgcgatgcac
     4681 cacgcaaagc atgttctcgg aaaaaagtac gaggactgct gctttatcgt atgccacatc
     4741 gacggcggca tttccgtatc ggcgcaccgg tacggacgca tcatcgactg caacgatgcc
     4801 gccgggggag acggccccta tacgccgacg cgcatcggct cggtctccgt ggcggacatt
     4861 ctcgaatacg tccgcgataa ggacatcgac gaggtcaaaa agctttgcac ccgcacgggc
     4921 ggctttgtga gccactttgg cacctccagc tccgacgccg tacataaaat ggccgaagag
     4981 ggggacaaaa aagccatccg cgtctgggac ggcatgaaat accagatcgt caagtacatc
     5041 ggcgcgatgg cggccgtgct cggcggcagg gtggacggca tcctccttac cggcggcctt
     5101 ctccgctttg aagacatcac gcagaccatc cgcgagcagt gtgagtggat cgcgccggtg
     5161 agcacctatc ccggcgaggt cgagcacgag gcgctcgccg ccggagcgct gcgcgtcctg
     5221 cgcggcgagg aagagcccaa gcactacacc ggccgccccg tctgggacgg ctggcacgat
     5281 gatgactgac gggcaaacca aagagccgca gcggttgaac tgctgtggct gatactgtgg
     5341 cagtgcatgg tatggcatgg catgaaaaga caaatcggaa tttacggagg taagtggtat
     5401 ggaggaacaa atccttaatt atttggatat gtcagaagaa caaaaagaaa aatctttgag
     5461 agatttaaaa agcataggct tttatcctgc atatggaaaa gaaaagacga tgaaaaagat
     5521 tatggacaaa tccatcgctg gagagatgcc gcaattctat tttgttttca gaaaagaaca
     5581 gttgataggt tatttgttta taattggtga tactcaaaaa tatcgagcct ttccgtggtt
     5641 ggctattagc aatttagacg aacttccgat gagagttacg aaggctttaa tggaaataca
     5701 gatagaggca tggataaaca taggcgatga aattatggcg gattttctcc gaagacaatt
     5761 aactaattac gaacatggaa taggacatcg acctgaaaat ttatgcagat aacttccagt
     5821 ttgttgagca tgaaaaaagc gccggagctt gagctccggc gcttttggca tattaaggaa
     5881 aatcagaaaa tcgcgacgac agcgccgttg taggtctcgg cgatgaagtt cttcacggtc
     5941 tcggactgga gagccttgac gagagcgacc gtcttggcgc tgtccttgtc ggcctcgcgg
     6001 acggcaacga cattggcgta ggtctgggcg gcgtcgccgg aggcgtcctc gatggcgaga
     6061 gcgtcggcaa ccttcagacc ggcggccagc gcgtagttgc cgttgataac ggcgagcgtg
     6121 ccggcgtcac tgttggcaag ctgggtcggg accatgtcgg cctgcacggg aacaacgttg
     6181 tagcccttcg cgtcaacgat atcgagatgg ctgacgccct tttccgcgga ggcatcctcc
     6241 ggcagggtga tgagaccttc ctgagcgagg aggagcagcg cgcgggtctc gttgctgtcg
     6301 tcggcgggga tcagaatggt agcgccctcg gcgacttcat cgagagagga aacgccgttg
     6361 ccgaaaagac cgaacggctc atagtggatg agaccggcgg agacgaggtg cgtgccgttg
     6421 ctggcgttga agctgttcag gtacggggtg tgctggaagt agttggcgtc aagctcgccg
     6481 tcttcaagag cggtgttggg caggacgtaa tcatcgaaaa caacgatctt gagatcgtag
     6541 ccttcctcgg cgaggatggg cttgatctgc tcaaggatct cggcgtgcgg ggtggaggaa
     6601 gcgccgacgg tgatggtctc cttcacggca ggctcttcgg caggggtctc ggccggagtc
     6661 tcggaggcgg gagcctcggt agcggcgggg gcttccgtgg cggacggggt ggaggcggtg
     6721 ccgcaggcgg ccagcgcgag cagcagcgcg agagcgagga caacagaaag aatctttttc
     6781 attttgattt ttctcctttt ctttggattc ggtgggtgaa actttttata tcatcaggcg
     6841 gaggtgcgtt tgtccgtctt gcggacaatg tacattccga cttcctgaat gacctgaaca
     6901 atgatgatgc acagcagcgc ggcgaaccag atgatatcct gattgaagcg ctggtgaccg
     6961 taaacaatgg cgatctggcc gagaccggtg ccgccgatcg tggcggccat ggcggaatag
     7021 ccgagcaccg tgaccatcga gatgaccgcg cccgtgatga gcgagggctt cgcttccggg
     7081 acgagcacct tggtgatgat ctggaatcgc ggcgtgccca tcgcttcggc ggcttcgatc
     7141 acgcccttat cgacctcctt gatggaggac tcgaccatgc gcgccacgta cggcgcggca
     7201 gcaatcacga gcgttacgat cacggccttg ttgccgagcg atgtgccgac gatgaacttt
     7261 gccaccggca gcatggccac catgagaatg atgaacggca cgctgcggaa ggcgttgatg
     7321 agaatgctga gaattttatt gagcaccggc atcggatgga tgccgtcctt atcgctcacg
     7381 caaaggagca gacccagcgg caggccgaga acataggcaa acaccgtgga gatcacggta
     7441 agatagatcg tctgccacac gccgagcaga aacagattct gctgccagag ctttacaaac
     7501 atatcggaga acatcccatc acacctcctc aaaggagacg ccgtcctgct tcagccacgc
     7561 gcgggcgcgc tcggcgctcg cttcgtcggc gggaagctcc aggatcgtct gcccataaat
     7621 tttgctgtcg atctcgcgaa tgtccgcgga tacgatgttg accggggcct ggcactccat
     7681 aatgagactg gcgacgacgg ggtgccgcgt cgtttcgccg ttgaaggcga ggcggattcg
     7741 ttttccgccg ggggtgaata ccggggcgat gctgacaccg gggaggatca gctcgcgggc
     7801 gatcttggac tgcggattgg tgaacacctg gctcaccgcg ccgagctcgg cgatctggcc
     7861 acggtcgatg acggccacgc ggtcgcagat ctggtcaatg acgcgcatct cgtgcgtgat
     7921 gacgatcacg gtgacgccca tcgtgcggtt gatctccttg agaagagaga ggatcgactg
     7981 cgttgtcgtg gggtcaagcg cgctcgtcgc ctcgtcgcag agaaggtagc ggggtttcgt
     8041 tgccagcgcg cgggcgatgg ctacgcgctg cttctgtccg ccggagagct gcgagggata
     8101 cgccttggcg cggtcggcga gaccgacgac ggaaagaagc tccatagcgc gctccacaga
     8161 ctcctttttc ttccatccgg cgatctccag cgggtagcac acgttctgga gaacggtgcg
     8221 ctgcgcgaga agattgaagc cctggaagat catcccgatc gagcggcgga tgagcagcag
     8281 ctccttctgg gggagcttgg tgagatcctt gccgtctacg atcacggagc cggaggtcgg
     8341 acgctccagc aggttgatgc accggacgag cgtggatttg cccgcgccgg aaagaccgat
     8401 gataccgaat atttctccgt cctcgatcgt gagggaaatg tcatgcacgg cgtggatctg
     8461 cccttcgccc tcgccgaagt ccttggtcac attctgaagc tcaatcaaaa gcgaaccctc
     8521 ctaacaaaca aaaaagcagg ctgccatttt ggcatcccgc ttcacaaaac tgcactccgg
     8581 atttcccgga ctgcgccttt ttatccatga aaagcagtca gcaaatggct atcgaaacgc
     8641 gcagcaaagc tgcgcatgca catcatcatg ttcatgcaca tctcggtcat catgctggcg
     8701 aacgtacggc gcatcggctg agctccttcc ctcggtaatc aagttgtctg cattatatcc
     8761 ctacggtttt gacctgtcaa tacactttaa caccgcaaag acagaatcgg cgaaaaaaca
     8821 caaaaccctc tcccgttcgg gaagagggtt tgtgaataat cattcaggat ttatactcaa
     8881 acgtcatgcc gcaaatggaa atggtgtcgc cgtcgcggat gccgagcgcc tccatgcgtt
     8941 tgtagatccc ggcgtcacgc agcgacttgt cgaaatacat ccggctctcg tagtcggtga
     9001 agttgacggt cgaaacgagg cggtccatcc acgggccgga gacgtaccag acgccgtcgt
     9061 cctcttcgat ctccacatcc tcgggtgagc cgagatccgg cgcgggcgga acgtagtccg
     9121 gctcgtacac cgcgatcggg gggagcgtgg agagcatggc agcagcgcgc tgcataagct
     9181 ccttcgttcc ggcggtcgtg gcggcggaga gctcaaagaa ctcgtagccc tgcgcctcga
     9241 cgtgcttttt cagccgttcg atcggctcgc ggtcgtcccc gagcaggtcg cacttgttcg
     9301 ccgcgacgat ctgcggacgc gacgcgagcg caggggagta gtccgcaagc tcgcggttga
     9361 tcgtctcaaa atcctcgatg gggtcgcgcc cctcgctgcc ggatacgtcc acaacgtgaa
     9421 tgagcagacg gcagcggtcg atgtggcgga gaaaatcgtg gccgagaccc gcgccctcgc
     9481 ttgcgccctc gatgatgccg ggaatgtccg ccagcacgaa ggaagcgccc tcggccacgt
     9541 aaacgacgcc gagattgggg gagagggtcg tgaagtgata gtccgcgatc ttcggccgcg
     9601 cgcgggaaac gctcgcgagc agcgtggatt tgccgacatt cgggaagccg acaagaccga
     9661 cgtccgcgag cagcttgagt tccagaatga gctcgtggct ctcgccgggc a
//
LOCUS       DAWAAE010000073         9662 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3181_length_9662_cov_3.110024, whole genome shotgun sequence.
ACCESSION   DAWAAE010000073 DAWAAE010000000
VERSION     DAWAAE010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_9662_cov_3.110024"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2825..2834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3411..3420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1923 a         2944 c         2555 g         2220 t
ORIGIN      
        1 gtgccgcagt acgggcaccg cgcgctcgcg ttcacgtcga gcggcgcgcc gcagcacagc
       61 aaacagagca agaactttct tcaaagcggc atcctatccc ccatcttttt ctatgcggga
      121 tatacgcttt caattatata ttttgcgccc gcgcatggca agagcgattt ccctttccgc
      181 cgcatgcttg agcacaaagc agccccgccc ggtacgcgcc cggacggggc tgctcaatct
      241 gccatagaag cctcgcagag tttgcgcccg caggcgcaaa taaagtttta accattttct
      301 ccggcgacat gtgcgtcgga gaaaatactt ttcggctcgc aaacgtgcga gcgtaagcga
      361 gtgcgctgcg gcgagccgca gccccttcaa acgagagccc aacgaagtgg gtctcgtttg
      421 acaagcttac atttttccct gcttgcgccg aagggagagg cagtcggcga tcatggcgat
      481 aaactcgctg ttcgtcggtt tgcccttaat gccggaaacg gtatagccaa aaaacttttg
      541 cagcgtttcc acatcaccgc ggtcccacgc gacctcgatc gcgtggcgga tggcacgctc
      601 tacgcggctc ggcgtcgtgg aaaacttctt cgcgacctcc ggatagagca ccttcgtcac
      661 agcgttgatg gcgtccatgt cgttgatcgt gaggatgatc gcctcgcgca gatactgata
      721 ccccttaata tgcgccggaa cgccgatctc gtggatgatg tcggtaacga cgctctcgag
      781 cgaggcgttg tccgccttcg cctccggcgc gatcgctgcg gagcttcttg ctccggtgac
      841 agacagcggc tttttgcccg tctgcgcgca ctgccgcaga cgctgtatca gctccggcgt
      901 gtcgcacggc ttggagagga agtatgccgc gccgagctcc gcgcactcgg caacgacatg
      961 tgcgttgaca aacccggtaa gtacgaccac cggcggcgcg gagtccttct cggcgagctt
     1021 gcgcagcacg cccagtccgt ccagccgcgg ctgcaccagc tccagcagca caacgtccgg
     1081 gcgcagcgct tcgatcttgg caagcgcatc cgaaccgtcg gaggatgtcc ccgccgcttc
     1141 catatccccc tcctgtgaaa gcaggctctt catcaacagc tgaaaatcct cgttcgggtc
     1201 ggcgatcagc acgcgtatct tggtttccat aatattgcct ccataattat gaactgcaag
     1261 cagtttgctg atgttttttt atgcttggct acattgcttg actaatttag cacaaaacgg
     1321 cggcgcgtcg caggaaaaac ttgtcgaaac tgtcttaata tttgttcgcc gccttcccga
     1381 cgatttcgac aagcggttgc aatgatttac ataatatctt atcaaaatat gcagtgtccc
     1441 gtcgaatctc cacgcctgtt tttcgccggt attccactct gcgcagtttc tctgccggaa
     1501 attccgtgtc tttcgccgca gcggtcccga gccgggcgcg ggatcttctt ccgggaaggc
     1561 gcgccggatc gtgctatatg agaaaaaacc ggccggcggc gtggctcttc aaaggctttc
     1621 cgatggaagg gttttcccat ggacggctac cggaaaaaca aaatggcgtc gctccgcaca
     1681 gcaaaacgca tacaatgatg caaggcccgc accaaaacgg tgcgggcctt ttttacgccg
     1741 tgcagttacc gacgtatcag ttcgtagata tagatctttg attttggatc gtatccgtag
     1801 cagtaaaagc tgctgccggc cgttgtgaga atataattcc ccacgacatc ggccacatcg
     1861 cctttgtgcg gcagcttcgc aacgatcttc agcgtcgccg gctccaccac atagatccgg
     1921 tgctccccga ggatgagcag ctcacccgtc gggagaaacg cctccggata cgcccccgcc
     1981 gggagtgcca tagtccttcc gcggtactcc ggcggacatt ccccggaaaa ggtcactcga
     2041 accggcgttc cgcattgcag gacgaaccgc gtatcgggac agcgcggtat gcgtccctgc
     2101 gcaaaggtct cccagtcgga aggttccagc gcgccgtgcg caaaatgcac cggcgtcaga
     2161 atgcgatgcc gctcgtcctc gtccggatca ccggcgtagc cgtgcagaaa gaagtgcggt
     2221 ttgaccgtga tacgctcgtc ctgaaatcta tagaaaaaca gttttcccgg atactcggcc
     2281 gcaagccgca gcgccatcgg ggcgcttttg ccggtcagaa gcttttcctc ttcgcggtac
     2341 gcgcggtaca ggcgctcgct ccgctcgcgg cagagtgcct ccggctcatc cagcatttcc
     2401 ggaacgaacg taagacagac gccaaaatac tcctccagca gcgcgatctt gcgttccgta
     2461 tccgcgcagc tgaggctatc ggagaggcgc cgttttcccc cgctttcgta cccgacgagc
     2521 gccggaatgc cgtacagtcc tttgttcgcg gaaaattcat cgtccgagta ctttgccgcg
     2581 accttcccgc cgcggaaaaa cacgaaccat atcgcctcgc tgtcaaatac gttgaaatac
     2641 aggaccggcg catccgtcgc gcgggaaatg cgccgcgcgg cctcgtacgt ccgttccggg
     2701 tccgtctcgg aaaagtcgtt cacacaggtg aaccaccccg cagaaaatga gcgaaaggaa
     2761 agctccgcat cctccggagc cgccgttccg aaaacatgaa tgccggtcag agacatgccc
     2821 atagnnnnnn nnnngccgca ccgccttttt cttttatcct atcataaaag gctgcgcgcg
     2881 caaaggaaaa agacggtccg cagaccgtct ttttccttac gaagccgctt cgagcatatt
     2941 ctccatggag atgccgtatc ctttggtggg gtcgttcacg agcacatgcg tgaccgcgcc
     3001 gacgattttt ccgttttgca ggatgggtga gccgctcatc ccctgcacga tgccgccggt
     3061 cacggcgaga agatcgccgt ccgtgatcgt gagcgtgagc ttcccgccgt cgccgctgcg
     3121 cgcgatgcgc gtgatcacgg cgtcatacga tcgcacgccg ccggtactga cggtggagag
     3181 gatcgtcgcc tcgccgagac ggatctcgct ctccgcggcg accggcatgg ccggaagcgt
     3241 gccgagcggc gtccggctcg tgccgaaaat gccgcggacc gtgttctcct cgatcgagcc
     3301 gatcggcgag gcgctgctcg cgccgccgca cagctctccc ggcgcgccgc gctcgcccat
     3361 aacgacgccg gtgacgtccg cccggtagat ctccccgccg gaggcgctca nnnnnnnnnn
     3421 tcatcagctc gccggtctcc ggcagaccga cgccgtggcc gagcgcgccg tactccccgg
     3481 tagcgggatc gtaaaatgtc acggtaccga gcccgcgcac cgcgtcgcgc agccagagcc
     3541 cgatctgata cgttccgccg ctgccgagct tcggcgtaac ggcaaatgtt ttcgtctcgc
     3601 cgccgcgctc gacggagagc gtcacgccct cgcccgaaaa ggccgccgcg cggcgcagaa
     3661 agtcctcggc gcccgtcacg cgctcaccgt ttaccgcaac gatcttatcg cccgcctgca
     3721 cgcccgcttc tcccgccggg cagcacgcgc cgccctccga ggcgatatcc gcgagcgcgg
     3781 acaccaccac gccgtcgcac cgcagcgata tcgccaccgc ctcgccgacc ggcacgagcg
     3841 cgtcgcccgg ctccgcggcg agcgccgcgg tcgtcagcgc cagtaccgcg gcaagcaggg
     3901 cgaatacata ttttatcttt ctcataggac tgccaccctt atccttccca tgcttttgca
     3961 tgtttcgcgc cgcaagcagc ttctttgcgg cggcctattc agtatgtctg tcgagaagga
     4021 aaagaaccgc gaaaaatttg tcctctgttt ccatcgactg tttcacattt tctgttttta
     4081 ctatggtatc cttccgtttt tctctcatgc ctgccaaaat tttttcatcg tgaggaaatg
     4141 tcatgcagat tttgaaaacc ggctccaccg gcgcggaggt cgcgcttttg cagctgtcgc
     4201 tccgccgcgc acagtacggc gctcccgcgc ccgacggcgt tttcggcggc gcgacaaaaa
     4261 gcgctctgca ggccttccag cgctcgcacg gcctgacgcc ggacggcata ttcggcgcgg
     4321 agaccgagcg tgcgctcgcg ccatggcttc gcgggtacgc cgtgcacacg gtgcggcccg
     4381 gcgatacgct cttctcgctc gccgagcggc acgacgcctc gctcggcgcg atcgaaacgg
     4441 caaacccgtc gcttgacccg ttcgcgctgc ggcccgggca gcggatcacc gtaccgctgc
     4501 cgttttccgt cgtgccgacg gatatcccgt ggtgcagcgc gctcatggac tacgccgtgg
     4561 acgggctgac gcaccggtat ccgcagcttc gcgccgagag catcggacgc agcgccctct
     4621 cccgcccgat ctgggcactc acggcaggcg acgggctgcg ccgcgtgctc tacagcgccg
     4681 cgcaccacgc gaacgagtgg atcacaacgc cgcttttgat gaagtatctt gaaacgctgc
     4741 tccgcgcggc cgccgcgggc gagaccgtgt tcggctaccc ggcggaggac atcctcttcc
     4801 gcgccgcact cacgctcgtg ccgctcgtcg atccggacgg tgtcgatctc gtgacgggcg
     4861 ctctgccgga gggcgaagcg aaggagcgca ccgcggccat cgccgcagag ttccccgcga
     4921 tcccctatcc ggacggctgg aaggcgaaca tcgcgggcat cgatctcaat ttgcagtacc
     4981 ccgccgggtg ggacaccgcc cgcgcgatca agttcgcgca gggctacgac cgccccgcgc
     5041 cgcgcgactt tgtcggcgaa gcgccgctca cagcgccgga ggccgccgcg cttgccgcct
     5101 ttaccgaagc gctcgacccc gcgctcgtac tggcgtacca cacgcagggc aatgtcatct
     5161 actggaagta cttaaatttt gagccggagg gcagccgcgc gctcgccgag cggttcgccg
     5221 ccgtgagcgg ctacgtctgc gaggacacgc cctacgcctc cggctttgcc ggatataagg
     5281 actggttcat tcagaatttt gaccgccccg gctacacgat cgaatgcggc ctcggcgaaa
     5341 acccgctccc gctctcacag tttgaaagta tctggaacgc caacctcggc atcctcaccc
     5401 tcgccgcgct ggggatatag acaaagaggg cgtgccgcag cgaattgcgg cacgccctct
     5461 tttatgcaat ttctttgtac cgcgtaaaaa cgtactcata gactttctcg cggtattccg
     5521 taatgtcgtc gttattatag cattccggca gcgtctccca gagaacatcg cggatcagcg
     5581 tatcgacaac cgcccgcgtt tcctgcttat ccgtccaatg atccagttca gagattttcg
     5641 tcttgatttt tcggagaaga tccgacgccg ccgtcttgag ctttttaata tcgtttttac
     5701 tcaaatcatc acggaacagc atgtcgtaca acgaaagctc ttcgtcactc gcgaagccct
     5761 cccgcgcgta gcgctgctcc tcccggctca tacgatcggc gagatccatc agatccagga
     5821 aggtcttttc gatcgtcgct ctgtcctgct cgccgttgta gctctggata atctcacggt
     5881 atcgttcgta gtaatcgatg cgcttcggat tggcaaacac cattttgtct agttgccgcc
     5941 ggatcgcctc atccagatcg cgcatgagca ggttcttccg ccgggttttg gcaaactcgc
     6001 ggcggagcag atcaaagtcg atggcgctga tgtcgaaacg cggcggcgcg gttatggcgt
     6061 ttggctgctg cggctcaatt tccacataat cgttaatgat ggcgttgatc tccaccatta
     6121 agtcggtcgt atcggtatgc tttctctttt tttgcagcat accgtaaatg gccgcaatcg
     6181 cctcacattg cctgcgcata tgtccggtta cgtcgtctcg atccgtatat ttcatcaggc
     6241 gcttcagttc cgaggcatag gtcgaaaacg atttcttatc ctcaacggaa ccgcacacgg
     6301 cgttggcggc atcctgcaaa tacgaaagct ttgcaaaacc ttctgctgcg atcaacagac
     6361 ccagatcgat cttccgttcg gagagaaacg tttccgtttt ggcaaccgtg tcgccgatcc
     6421 gcgcgatcag tttctcttta tccactgtcg ggtcagcacc gccaaggccg ccggcatttg
     6481 ccgtatagtc tgcaagcgct tttcgcagcg cttttacaat gcctatgtaa tcaatgatca
     6541 gaccgttgct tttcccctct gccacgcggt tggcacgggc gatggtctgc atcagcgtat
     6601 gcgctttcaa tggcttatcc aaatacaggc aggacaggca cttgacgtca aacccggtga
     6661 gccacatggc gcagacgaat acaacgcgga gcggattgcc ggaatcctta aactctttgt
     6721 ccaactcgcg gttttccatc ttttcgcggt gcgtcttgat atccagtccc cattttctga
     6781 aggtctgtat ttcgttttgc tcctgtgaaa tgacgaccgc catttccgtt tcctgcatcc
     6841 agcgcagctt gcgttccagc tcctgcgcct cctgctgcgt cgccgactta atctttgcct
     6901 tgagattttg aatttccgct tgccaatatt cctgcacgaa gttatacatg cgcacgcagg
     6961 tcactttatt caggcagaca aacatcgcct tgccgctcgt ccagagatcg gagtagtggc
     7021 cggcaaagtc tttggcaata gaccgcagac gcggctccgc cgtcagcaga tggatctcct
     7081 tggcgaactc cgattcgagc ttgtcctgct gatccacgtc gagatccgcg ttttctatgg
     7141 cgtccagaat ccgatttgtg atctccgggt tgtgaaggtc aaggatcttc tctccgcggt
     7201 tctcgtaata cagcgggaca gtcgcgccgt cctccacggc tcgcttgaag tcatagaccg
     7261 aaacatagcc gccaaaggtg cgggcagtga tgttgtcgct cgacaaaagc ggcgttccgg
     7321 taaagccgat gcgcgccgcc gtcggcagca gcttcatcat gttatcggca aagataccgt
     7381 attggctgcg gtgcgcttcg tccgacatga tgataatatc gtggtcggga tagatcggcg
     7441 cggcgtcggg cttattgaat ttctggatca gcgtaaagat gaagctggga ttccctttca
     7501 gtttctgcac gagatcgtcc ccgctcgcgg cgataaactg cgctgctttg gtttttccga
     7561 gcagcccgca gttttcaaac gtgtcgctga tctgcttgtt cagctcgtcg cgatccgtca
     7621 agatcacgaa cgtcggcgaa ccggcaaatt ttcgccgaat cttctgcgcg aggaacacca
     7681 tggaatagct cttgccgctg ccctgcgtat gccagaacac gccgagcttc ccgtcgttca
     7741 gcttgcggcc ggcataggcc ttcaccgcct cattcacgcc gagatactgg tggttgcggg
     7801 caaggatctt tgccgtgcgc ccgccggagt ggtcgtaaag gataaagttt tccagaagat
     7861 cgaggaagtt ttccttccgg cagatgccgc gcagcatcgt ttccagcgca acgctgccgg
     7921 ggtcggactc cgccaaacgc ttccactcgt ggaaaaactc gtatttgctg ccgagcgtgc
     7981 cgatcttcgc ctccgtgccg tttgagagca ttagaaaggc gttgtagtag aaaagctgcg
     8041 ggatcgtatc gagatagtcg gtgtaattgt cctcataggc gttctgcacg tccacggtat
     8101 tcttcttgag ctccacgaac agcagcggga taccgtttac gaagccgacg atatccgtcc
     8161 ggcggcggta aaggtcgcct tgaattttca gctccttgat ggcgagaaat tcattattct
     8221 ccgggttttg aaagtcgatc accgcggctc gcttcgtttc cggctgaccg ttcggctttt
     8281 tcaccgtgac ggggatgccg tcccggagga gaaagtactt ttcctcgttg acttgcagga
     8341 gggacgccgt ggaaaggcgg ctttccagcg ttttctgcgc ctcggaaagc tgcgaatccg
     8401 tcatccacgg gttgagccgc cgcagagcat tctgaaaata ccgggtcagc agtatctcgt
     8461 gatagcttgt ccgcccgaac gtgccgtttt tgccgagcac ttccgtattg taagcgaact
     8521 gcacatccca gccgagctca tcccggagca gatccccggc gcttttctgc acgaggatgt
     8581 tttcagaata gtcgtagctc atttcttcac cgcctaattt attgcatgaa taatatcgag
     8641 ttttagttct tcatctgttt cgtatgttcc atccataaca gcgatgatga ttcgatggag
     8701 caaatcttta tcaattttcc ccgccgcaac ttgatagctg atgttttcca taactgtaaa
     8761 aaacgatttg gccacagcca tatacccatt aaaaaggaga aattgagcgc ttaatgttat
     8821 tgccagacgc ttattgccat cagcaaagca gtggaattca cacacgcaga aaaacaggtg
     8881 tgtcagctta tcatcgaaag ttgggtagta atcatcgttc tggatgtttt gaagaacgct
     8941 ttctaagcgg ccaagatcaa aatgctcata tgtaccgccg ccgctgtatg ctatggtttt
     9001 tgcatgaatt gcttttgcct gttcaagcgt gagataaaca atcccatcca tttactcgcg
     9061 ctccttaagt ctctttaaaa cggcttgatt ttcttgcatt aaccgctcaa gcacatcacc
     9121 cgaagcacca aggaattttt ggtattcgtc tttgtcaagg ggctgaatat attcagacag
     9181 ctgatagtga aaagcatccc gcagcgccat gtctcgactg gccattttca cgcggccgcg
     9241 ctgaatcaac ggcttccata gtgccatgtt ttcaaactcc cgaaagagtt cttctgtttc
     9301 attatatgaa agggcgcggc caagctcctc actttttctt ctgattgcat ccgccaagcc
     9361 gctctcgtat gccgcgacga tatcgagaat ctcactataa aaagtgtctc ttactttttc
     9421 actcgccttc aaatccagaa tctttttgta ttcctttgcc ttttccttga aaatactaat
     9481 atagatcaaa tccgtaaagt gcgcgtattt ataacgatta tcttcaacgc aattttttaa
     9541 tgcatccgta aactgcctac gataattatc ctcctgcaaa gaggaaaaca caaaatcttt
     9601 atcgcgttgg ttgatgtatt ttgttccacc gccggttttc cgattaatca aatcaataac
     9661 ga
//
LOCUS       DAWAAE010000074         9519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3242_length_9519_cov_3.634615, whole genome shotgun sequence.
ACCESSION   DAWAAE010000074 DAWAAE010000000
VERSION     DAWAAE010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_9519_cov_3.634615"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2107 a         2650 c         2714 g         2048 t
ORIGIN      
        1 gagctttttt ctttatctcg ccgcgccctt tttcgagagc tgccgatttc aaatctttca
       61 aatatttcaa atcttacatt aaaaatcgca ttaaaaattt ttgcgttgat cgtagaaaga
      121 agagggcgaa aatcgccccc ctctatttta aaaatttctc gattgtcttt ctggcgcgcc
      181 caagttcctg cgaaacgtac tgtggcgtac atccaagtgc gtcgccaata tcataaacag
      241 acagctcagc gctgcgcata gtaaccataa gccgttgcgt gggcgtaagc cgcttcatca
      301 tgtcttcaag cgcgggatgc tcgtcctcct tcccgacagt ttcctcgaca agtaatcggc
      361 tattcggcga aacgaaatca tctaacggca gtgccacgat ctgcggcttg cagcaatctc
      421 gaatatatcg gcacaccgcc atgaaaatgc ccttgaccgc aaatattgag aatttagctc
      481 ctttttccgg atagtattcc gcacacgcct gccatagccc tatcctgcac acttgtagaa
      541 catcttcatc aactttaaga ctcgggaaat actttttcaa ggtaaaccaa acaagcgact
      601 gattttcttc atacagtttc tctggcgtca tgtttgaaga gcctccacaa aacaatagct
      661 ctgcggcggg tgcttgatac ggtggcacca tccgctccac tcgttccaga aggaccagat
      721 gcttatcatt gctgatttac tcatcggttc tcctttctcc gtagctgcaa aagtcatccg
      781 ggccgacaat tcccattcgt gcagctccgc attctccgta acagtcactg aacatgacgt
      841 gtttgcagtc cttgcaatgt acgaccggct cataccccag ctgcaccgcc atgcgcttaa
      901 actggctgca ggtggggtgg tcaatatcac ctgttggagc gtctgcaaga agcatttgga
      961 ttttcttgtg gacgacctcg ttcacgagcc ttgttgccat gtccgtgatc ttcacttggg
     1021 tggaaatgaa tgcggcggtt tcatcttccg ccattaaatc ggcgtcaatc agtcttgcca
     1081 ttccgaacgc cccccatccc cccgccccat acagcgagga acggcggaga gctgccagcg
     1141 aaacggcgaa agctgctgcg agcaactgga caaaaggtat agaaactgtt tagatggttt
     1201 cagaatatat ggtagattta tttatacgtt cttgatataa ttattctcaa tgagatggag
     1261 ttgaagcaaa atacattgga cagaagggag atgctaaaaa atgttatctt tttatctgaa
     1321 agaatctttg gacattacaa agattaaaga ccctgagcat tctaaagagt ccgaagtcga
     1381 cttttctggt aatttaggtc gaggagtatt gttgttgtcc atcctttttg tgattgtata
     1441 tgcaattcta ctggtcaaat ttcgtagata tataggagcc tttgaagcat ctttaacacc
     1501 aatgataatt gggttgatta gctcaatttt aatattgcca cttcatgaat tgctccatgc
     1561 catttgtatt tcttcaaaaa ataatgttca aatttacagg gtcaaatccg gtcttatgac
     1621 gtggtttact gcacctataa ctaagagaag atatttagga atgctgttgg ttcccgtgtt
     1681 actgttggga tttattcctt caatatgcac ttttgtcatc ccggaaacaa tgccagggtt
     1741 tatcaccttt gtattgattt tttcacttgg taatattgga atgtcgtgtg gagatttgac
     1801 aaatttcata ataacagcga ttaaagtaaa gaaggggaat aaagtccttc cgtgtaaaaa
     1861 cgggattttc aaagttcagt aatcgcggag ttgctcaaaa tgctgtttgc cacctgcaag
     1921 ttttgaaaaa ctgaacaccg attatcgaaa agagtgataa aacttattta ttggaatggt
     1981 tgcgatttgt ccgtctgcct agcatatatg gtagaaagga agtgtacgat atgagcttaa
     2041 aagaattttt gctcgatgtg ttgtgccgga cggtcaacgc ctcgtggtat cgaaccctgg
     2101 ggtgctcgcc agaagaggcc tcttttttgg ctgacatgga agccaacaca gcgttcttta
     2161 aacaaagcga gaaaagaaat ccttcctatg cgctttctga actccgctgc aatttgtcag
     2221 aagaagatcg taatacggaa cgccccgaaa cccttgagag catctgcaca gcatcaggcg
     2281 acgttgatgc gtatattgcc gaaaaatcca aaaccgatgc gagatattcc gctgttcaaa
     2341 aggaatttca gcggatcgac gcttccaaaa ccgcgctgga agaacgccgc cgaaaactct
     2401 atgatgaaaa ccccggacta tatgaaaggg cagaaaaatt ccgtcaggaa gccgcccgga
     2461 agcgcgaaga agcaagaaag aagaaccgtt aacctgcctc gcctccgttg gtatgcatat
     2521 ttatccccct tacggttttg ggctattcta ctcccatcga atctactcac atcgaatcaa
     2581 aagaagtccg atccggaaag gccgggtttc tttggacaag gcagcccagc aaaaacggga
     2641 ggacgatctg tcctcccgtt ttcagcatat tgaattgctt atttcatctt atatttcccg
     2701 cgtcccgcgc tggaaaggcc catctccgtg ccgttccaga tgcggataaa gttttcgcgg
     2761 tgcttgatga ggatggcgag cgtcgccacg cagaggatga cgagcggcag ccagccgctg
     2821 cggatggcgg tatagattgg gaacgagaca acgctcgtca cggtgccgac gacgatgtaa
     2881 tccgtcagca cagtgaggat aacgaccgcc gcgagaatgc acagcgcaac cttccagttg
     2941 atggcaagga tcatgccgag atacgaggca aagcccttgc cgcccttgaa tttgagatag
     3001 aacgggaaca tatgccccag cacgcacgcc acgcccgcaa tgaagccgag cagcggcacg
     3061 tccgggaaga tccacttgga gagctcgacc gcgagcacgg ccttgccgat gtcatggacg
     3121 gcgacgagca gtcccgcctt ccatcccatg agcgcaaaag cgttggaagc gccgagattt
     3181 ttcgacccgc cggcgcgaag gttcacgccc ttccaccggg agagatacag tgccatgttg
     3241 gaacagccaa tgaggtatgc gccgagaatg gtgacaatgt agggaagcat agcagccccc
     3301 tttctgcgga aggcccgcgg gaaaatttgg tttttctatc gaatcaagaa atccttaacc
     3361 ttttttatat tacgcgcaag cggcgcaagt gtcaagcgtt ggtttcccgc cggacatcct
     3421 gcgccgcgtg cgcataatat tctattctcc cgaaaatgtt cagatcacgc gaaaatcgct
     3481 ttgcatcgcc gggatattgc ccttaaattc tttgaaacaa tccaagatca gatccgccat
     3541 ctccgtgccg atctcgcgca cgacagggca gccgacataa aagtcgtacc cgcccgtgcc
     3601 gctggcgcgg tagctgttga ggcagatggt aaaggtgtcc tcttcgcgca cgtcctgccc
     3661 gttcacgcgc atggacgtca cccgctcgcc cacgggccgg gagatatcgt acacatatga
     3721 gacgccggcg tagtagtcgt aattataatg ttccaccttc ggccggagga aaacgtccga
     3781 aacgcagagc ctcccgtcct cgccgcggga gaaatactcc gcgctgcgct cgagcgccgc
     3841 tttgagaacc tttccggtga tctccagcac cgcaagcgtg ttcgtgtacg gatacgcgat
     3901 gagaagatcg cggcggcgca cggtcttggg cagtcccgcc acatcgtttg caaggctcgt
     3961 cgccgagagc tgcgctccgc tcgcccagag ctgcaccgca ttgaagagat cggcgagcgc
     4021 atttccctcc gacgccatac gcagcggcgt gtccggcgtg agagcgtgcg gcagcgtgcc
     4081 gatcacttca tcgagccagt tctgcgcgcc gtgctccatc tcggaaaact cctcgagcca
     4141 ttcgcggcgg cacgcgccgt gcgcggggac ggtctcgctc gtgatgcgct tttcttcccc
     4201 ggagagcacc gcttcgacgc ggatgaactc ctgcccgcgg tcggacggct gcacgacgaa
     4261 cgtgccaaaa agcgtctgcc ccggcacgga catgtgctgg tggccggtca gcaggatatc
     4321 aaaatccagt tcctgacaaa tgcggtaggc gacattttcg tgcgtggcgg agaggacgcg
     4381 ccctgtggca agatcgcgct caaagccgcc gtggtagacg cagagcgtaa cgtccgaaat
     4441 gcttttgagc ttttcgagcg ccgcagccgc cgccgggatc gggtcggaaa tgcggatgga
     4501 ggcgatatgc tccggccgtt cccagatgtt cacatagtcc gtcacgatgc cgacgatacc
     4561 gacacgaagg ccgttttcga gcgtgtggac gcgcgccggg tagagcggcg cgcagccgtt
     4621 tttcgcgctc acattttcgc acacgcaccg cgcgttcaga tttccgagat acgagccgag
     4681 atagtccgtg ccgaagttga aatcgtggtt gccgagcgtc acatagtcgt agccgcagcg
     4741 gttcatgatc ccggcaaagg cgcggccatc gccgatcgtg tcgtggcaga aggcgcccag
     4801 cggcgagccc tgcagcagat cgccgccgtc gatgacgagc gtattcccgt ccttttcaaa
     4861 gcggctcgcg catttgaaaa ggccgatatc ccgctcgtcg cggctgcggt agtccgtggg
     4921 gtaaatgtat ccgtgcaggt cggaagtgaa gtaaacagtc agcttcttat ctgccatagc
     4981 gtcttatccc cttgcaagtt tcgtgcggat cttcgcggag atccactcga tgatgagcac
     5041 gagcacgatg agtccgagca gtatcgcgcc cacctcgttc cagcggtagg agctcatcgc
     5101 aaagatgagc ggcgcgccga tgccgcccgc gccgacaaga ccgagaacgg tggcgtcgcg
     5161 caggttcatg tcaaagcggt agatgatcgt ggaggtgaag tccgcggtga gctgcggcag
     5221 gataccgtag cggatcttct ggaacgtggt gcatccggcg gcgtcgagcg attcgagaat
     5281 gcgcttgtcg agatcctcga tgctctcgat gaacatcttc gtcaccatgc cgatcgaaca
     5341 gagcgacatc gtgagaagcc ccgcgaacgg cccggggccg gtgacgcgga taaacataag
     5401 gccatacaca aacgccggga tcgtgcggat ggccgcgatc acggtgcgcc cgatgagcgc
     5461 gatcggcttc ggcacgagat tgctcgccga aaggaacgcc agcggcagcg agatgaccgc
     5521 gccgacgatc gtgccgagaa acgcgatgca gaacgtctcc agcagcagat acggcacgcc
     5581 gttcgtgccg agcgtgaaaa ggagctttcc cgacgggtgg aaaatgccgg agaggatgtt
     5641 ccccgccacg gcgaggccgt tgccggtggg gttgctcacg cgcacggcgc tgcccgacca
     5701 cgcgagcagg cacgcgacga tgaacgccgc aaggagctgc cggatccacg ttttcggcgc
     5761 ggattcgtag gccctgagaa ttttcttgtc catagcttcc cccctcaggt caggcgctta
     5821 cgcagccagt ggctgagcgc ctcgatgacg atgacggtca cgaaaagaac gatgaggatc
     5881 atgccgagac tgtcgtactc gcgccagccg atcttctcat tcataatgag accgagaccg
     5941 cccgcgccca cgtagccgag gatggacgca tagcgcacgt tgccctccag acagaagagc
     6001 gaaacggaaa gatacgtcgg cagcacctgc gggaacacgg cggtcgtgaa ggcctgcagg
     6061 cgtgttgcgc cgagcgcctc cattgcctca tacggcccca tatcgaccgt ttcgatgatc
     6121 tcaaaaagct gcttgccgac ataggcgaac gtgaaaacgg cgatggcgca cgtacccgcc
     6181 atcgtgccga gaccgaagat gtaggtagcg atcagcgcgc acacgagcgt cggcagcgtg
     6241 cggacaaggc tcagaaagag ccgcacaatg aatacgacga cgcggttcgt aacgatattg
     6301 cttgccgcgg cgatggcgaa cgggatggcc agcaccgcgc cgatgaacga gccgagaaac
     6361 gacattttga tcgtgtccca cagcggcgtc cagattttgg agagataggc ggtgttcggc
     6421 gggaacatgg ccttgagaat gtcgaaaaac ttgctgccgc gctttacgag cacggcgaaa
     6481 tcaaaccccg tcacacgcac ggagaccgcc gcgagcgcca ccacgatgag aataatgatc
     6541 ggcgcgcggc tgcgtttttc cttcacgacc gttccgtcag agagcgtgta ggtgcggggc
     6601 ttgaacacgc ggtcgtagag actcatgcgg gttcctccgt ttcatcctcg ccgtagattg
     6661 cggcgagaac gtccttatca acgttggcgg acggcccgtc atagacgatc ttacccgcgc
     6721 ggatgccgat gatgcggtcg gcatattcca gcgccagctc gacatggtga atgttcagaa
     6781 ggatcgtgat gcccatgtcc ttgttgatat gcacgaagtc ctgcatgacc tgtttggccg
     6841 tgacagggtc gagcgccgcg accggctcgt cggcaaggat gatcttcggg tcctgcgtga
     6901 gcgtgcgcgc gagcgccacg cgctgctgct ggccgccgga gagctggtcg gcgcgcatat
     6961 acgccttatc gagaatgccg accttgtcga gcgattcgag cgccttgatc ttgtccgatt
     7021 tccggaacgc gccgagcagc acgcgccaga acggcatatc cggcacgcac gcggccagaa
     7081 cgttctggat aacgctcgtg cgcgtcacga gattgaacga ctggaacacc atgccgatgc
     7141 cgcggcggaa acgccgcagg ctcttgccgg agagcgagct cacatccgtg ccgttcaccg
     7201 tcagcgtgcc ggaggtgatc cggtgcatcc ggttcaccga gcgcagcagc gtggacttgc
     7261 ccgcaccgga gcggccgatg atcgcagcga attcgccgtc ggcgatcgtg agatctacat
     7321 cgtccagccc gaccgtgccg ttgggatata ccttggaaac gtggtcaaat acaatcatac
     7381 ggactcctct ttcttatata gcgcgcgggg agggcggcac agcggaaggt ttgatgctgt
     7441 accgtcctcc ccgcagttgg gaaagtgtct cagttggcgg cggagagctc ctggatgagc
     7501 ttctgcgcgg cgcgctcgtt gtcatagtcg gaggactgcg cgatctggta gccgttgtgg
     7561 ctgtagatgg cgatgacctg cttgccggca tcggtattgc cgatgttgat gaacgcctgc
     7621 tgcagcgcgg cgatcagatc ggcatccatg atgggcgagt tcttgctcac ggagatggtg
     7681 tcgttgtaga tgggcgcggt gacgccgatg acgccggtct cctcccagat ggagccttca
     7741 cggccgaact cgctgttcca gcgctcggca tagtcgcggc gggcgtcggc ataggtcacg
     7801 agaacgtcga cctggccgga ggcgagacgc gcgaacgcgc tggcgtagga gtcagactgc
     7861 acggcgctgg agagatccga gatgcccttg ccgtagtggt cctgcagcca gagagcgggg
     7921 taaatgtagc ccgcgggaga ggacgtgccc ataacgctcc agttggcgcc gtcgaggtct
     7981 tcccaggtga gctcttcccc ggcgttgacc ttgtcctgca gctccttgcc cttttcggac
     8041 gggccggcga tgaacagagc gcggtaggaa accgcctgac ggtcggaagc ctcggtgggc
     8101 tggccgtcgt tccagtcctt cgcgttgtcg gaatccttgc tcagaccgtc gcgggtggcg
     8161 gtgagaatga cctcggcgcc gtcgtcatag agaacgtagg tgccgccggg gatgaggccg
     8221 acgtcgatcg tgccggcttc aagaccttcg ccgaccgctt catacgtcgt gccgacggtg
     8281 atctccacct cgccgacttc gtagccgagc gcggcgagct cgttcttgag catctccttg
     8341 agaggctcgg tgacggtgat gatctcctgc ggctcgcggg acgggacgaa cgcgaccttc
     8401 agggtgtcga tcttcttcgc ggtgggttcg gcggcgggct cttccgcagc gggagcctcg
     8461 gtagccgggg cttcggtgtc cggggtctcg gcggcggtgc cgcagccggc cagcaggccg
     8521 agcgtcatga cgaccgcaag caggagcgca aaaatctttt tcatcctttt tcctccgata
     8581 atactcttct tatttttttc cggcgcatac gcaaaaagca aggtgcgccg ttcctcaaag
     8641 cgagtatagc acaccccgat aaagaaaaaa tttccgattc agtaaaaatg ttagttcttt
     8701 gtgtcccgct cgaaatagta ggccacgagc tcttcaaacg caaggatcac atggccgaaa
     8761 aaggggttcg gcaggctccc ggcgagcgtc tgcttcgagc cgtccaccac gatgacgaga
     8821 tccgcgagcc gcgcgagcgc ggaatcggcg cgcttggtga acgtcacgat cttgtggccg
     8881 ttccggcggg cgtggcgcac gtcgttgagc accggctcgg tctccccgct ctggctcacg
     8941 gcgaacatga cgctcggcgc ggcctcctcg tcggtgagat cgtgcgccga gcagaagagc
     9001 atgtggtcat agaaatggac gttattatag accataaagc cgcagatgga gagccgctgc
     9061 gcgatatact cgcccacgat gctggaaaag ccctcgccgg tcgtgaacat cttcgcctgg
     9121 cgcgaagcgt cgagcagcgc gcagaagccg ttcaccagtt cgtccgaata gttgtccacg
     9181 agcgttttga gattttccgc gctgcgctcg ccgcgccggt ccatctggcg gctgagatag
     9241 taatagagct cgctgtagcc gtcaaagccg agacgcttgc aaattttcac gatcgacgcg
     9301 gcggagagga aattctcgct cgcgatctgc tggatgccga cgcgcttctc gccgcgctcg
     9361 atgtgcgcgg caatgcccgt gatgacgcgt ttttcgtcct cggacaggag gttggtcaaa
     9421 agaaaataac tgccgttcat agcgtatcaa gaaatccttc cccacaccgt tttatccact
     9481 ataccagacc cgccgccaaa gcgcaacgcc cctcttcgt
//
LOCUS       DAWAAE010000075         9480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3262_length_9480_cov_2.927427, whole genome shotgun sequence.
ACCESSION   DAWAAE010000075 DAWAAE010000000
VERSION     DAWAAE010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_9480_cov_2.927427"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1508..1517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2464..2473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6200..6209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2016 a         2925 c         2733 g         1776 t
ORIGIN      
        1 gccgcgcggc ggagatgatg gcgttggcct cggcgtggac gctgcggcag agctcgtacc
       61 gctctccggc ggggatgttc agctcgcgcc gggtgcagcg gccgagatcg gaacagttgc
      121 gccgtccgcg cggggcgccg ttgtagccgg tggcgacgat ctcgtcgtca cgcacgatga
      181 tcgcgccgaa ctgccgccgg agacaggtgc tgcgctccag agcggcctcg gcaatgtcga
      241 gatagtaatt ctccttgctg cggcggttgt ccatgggtat ttctccaatc tttttctttg
      301 ttaatcgacg ctatcccatt cattttactc ttggcgcaca agtttcgtca atgctcttgt
      361 ttttttatga aaaagattat aatataaata gtctcttgga gggaaggagg gaaacgccgt
      421 gcagcagaga aactatcagc gggagatgga cgcgctcatc gcgtcgttcg atgcccgccc
      481 ccggctgctt ctccacagct gctgcggccc gtgctcaagc gcgtgtctcg agcggctcgc
      541 gccgcatttc gatatcgacg ttttcttcta taacccgaac atcctccccg cggaggaatg
      601 ggaaaagcgg ctcttctggc agaagcacct gctcgaaacg gcgccgttcg gcaaaaatgt
      661 cggactgatc gtcccggagc gggacgagac ggcgttccgc ctcgccgcgg cagggctgga
      721 agcagaggca gagggcggcg cgcggtgcac cgagtgcttc gttctccggc tctcccgcac
      781 ggcggaggcc gcgcgcgagg gcggatacga ctatttcgcc acgacgctca ccgtctcccc
      841 gcacaagaac gccccgctta tcaacgccat cggcgaaaag ctcgccgaag atcacggcgt
      901 tttgtggctg ccgtcggatt tcaaaaagcg cgacggctac cgccgcagca ttgaactttc
      961 gcacgaatac ggactgtacc gccagagctg gtgcggctgc ggtctgcccg agaattctta
     1021 aatatcacca cagaaagagg taccttgtct tgaacaacaa cgatctccgt gaaaagctca
     1081 ttatccccgc cgtcgtcggc gcgctttatg ccgagctcac gatgctgctt gctcccatca
     1141 gctacggcaa tctgcaattt cgtatttcgg aagcgctgtg cgtgctgccg gttttcttcc
     1201 cgtatacgtc cgtcggcctg tttctcggct gcgcgctggc gaacatgatc tccgccgccg
     1261 ggatactgga cgtcgttttc ggatcgcttg ccacgctctg cgcgggactt tgcgcggcgg
     1321 cgtgcgggcg tgaggcgcgg cgcaccggcg cgatgccgtc cgcagcgatg cgcgttctgg
     1381 cgtgcctgag ccccgtggcc tggaacggcg tcgtcatcgg cgcggtgctc gcgtggagct
     1441 tcgcacgcga cgccttctgg cagagcttcc tgctcttcgg cgcggaggtc gcgctcggtg
     1501 aggcgggnnn nnnnnnnggc ggtgccgccc cactttgcct tgacttcagt ggcgtactgt
     1561 tcctgctcgg catgccgctc atctcgctca tcccccggat ctttcatttg gaaaggaaga
     1621 acgccgcatg aaatcgctgc gcgagcttta caccgtcggg cgaggcccgt ccagctccca
     1681 caccatcggc cccacgcgcg cctgctgtct cttccgcgag gaacacccgg acgccgaccg
     1741 gtatgaggtc gcgctcttcg gctcgctcgc cctgaccggg cgcggccatc tgaccgacgt
     1801 cgccgtgcgc gacgcgcttg cgccggtgca caccgacatc ctctggaaca cggagcgcac
     1861 cgacctgccg cacccgaaca cgatggagct gcgcgcttac aaaaacggcg cgctcaccgg
     1921 cgagatgacg gccctctccg tcggcggcgg cgcgatctcc atccccggcc ggcccgacac
     1981 cgagtcgccg gaggtctatc cggaaacgag ctacgcggag atctccgcct tttgcagcgc
     2041 gaaccggctg cggctgagcg attatgtgtt tctgcgggaa ggagaggaga tccggaacta
     2101 tctttatgat atctggaaca caatgaagca gagcgtgtcc gacggtctca cccgcaccgg
     2161 cgagctgccg ggcggactgg aggtcgagcg taaggcgcag tatctctaca accgccgcca
     2221 tatcgacgaa agcccgcaga cgcgcgaaaa ccggctcgtg tgcgcgtatg cgttcgcggt
     2281 ggcggagcag aacgcctccg gcggggagat cgtcacggcg ccgacgtgcg gcgcgagcgg
     2341 cgttatgccc gcggcgctcc tttatatgca ggaggagcgc ggcttctccg atgaggcgat
     2401 cgtccgcgcg ctggccgcgg gcggcgtgat cggcgatctc atcaagacga acgcctccat
     2461 ctcnnnnnnn nnncggcgcg gagtgcggct gccaggcgga gagcggctcg gcctgttcca
     2521 tgaccgccgc ggcgctcggc gagctttttg aaatgggtct cggccagatc ggttacgccg
     2581 ccgaggtcgc catggagcac catctcggcc ttacgtgcga cccgatcggg gggcttgtgc
     2641 agatcccgtg catcgagcgg aacgccgttg ccgcgatgcg cgccatcaat gcgctctcgc
     2701 tggcaaactt cctttcggag acgagcaaga tctccttcga caccgtcgtt cacacgatgt
     2761 atgaaacggg acgggactta aaccgcgttt accgcgagac cgccgagggc ggtctggcga
     2821 aaaattacca tcctgaaaaa taagtcaaga ccaccggctc agccggtggt cttgactata
     2881 cccccgcttt tttctgcggg aaacgcttcc aaaaaagctc attcgtccgg cgtcagaaaa
     2941 atgccgtccg gcgtcgtctc gccggtgtag ccgaaccggg tacagaattc ctccgcgtac
     3001 tccgcatttt tctccccgac ccagacggca tccggccgct ttcccgggtt gagcgagtag
     3061 tacatctcca gacggtcgag cgtgacgctg tccacaccgg tcagccacgc agaaaaagcg
     3121 gcgttccggt aattgcccat aaacatccac gtgtttctcg tcagcggaag aacctcccct
     3181 ccgcgggaaa gcgcttccag ctttttcatg acccggaccg tcgtcgcgta ttcctctgcg
     3241 gcggttgggt tggcaataat ccctttctgc ggtccctctg taatgtgttc cgtttgaagg
     3301 gcgatcctgt cctcatttcc cttttgatat atcacgtatt catatcgcag aaacgccgtt
     3361 ccgccaattt gcagaagcgc cagcgcggca agagtcgccg ttgaaaagcg gcggagccac
     3421 gccttttcct ttttgaagat gccgcgtgcc gtgacggcga tcaccgtaag actgcccggc
     3481 actgcgcagg ccgcagcaga aaaaaccgca tactgtctct ggttggacgc catgatgatc
     3541 agtccggaat atatcatgca cgggagccag aacaggaaag cgaagcgccg gagcgtttcg
     3601 gctctttccc ggtgggcgat aaagaagcaa agcaacgcca gcacattggc tgcatacatc
     3661 atgaaattgg gatcgttcca cgcactgacg tacaacactt ccgctgcaat cgtcaatatc
     3721 gttccgatga gaaaatacgc cggagcgtgc ctctcccggt gtttatccaa aagtgctgcc
     3781 ggtatcatca gcaggaccag aaccatgcac gctcccggac gccaatgccc atgataaaaa
     3841 acaagccggg gataatccat cacagcttgg cacacggact tcaggagaga cgtgcgttcc
     3901 gggtgcgcgg gatccgaaaa aattcctttg agtatttcat ccaggcggga cagatccgcg
     3961 ccggcaagtc cgacaaagaa aaagacgatg gcaaggcctg ctgctccgag cgtgaaaaga
     4021 ccgaatgggc gcggataaaa cgccgggagt tttttctttt tccgcggaat gaacacaaaa
     4081 agcgcataaa gaagatatac agcaataaga tacgggcagc acagaaccga accggcaaag
     4141 gaaagccccg cgagaatata ggctgcgcgg ctttcctctt ccgcggggac aagcagcgct
     4201 cccgtcatag ccataaggag aatgccgaga gaattatagc tcagcgcgtt gatgccgtaa
     4261 ggcgcatata ccgcaagagc aagcgcaccg gcggccgcgc ccagagaatg atagcgtctc
     4321 aaacgcagat aaactgtggc tgccacaagg cactgcactg ccacatacag ataccgcagc
     4381 gcaagataaa tgcctgcggt gccgccggtc agccgggtaa aaagcagcac gggaagatac
     4441 agcagcacac tggagagctg cgacatatgc cattcgtcaa cgagcagcct gtcgccctgt
     4501 gtcagccggt aggcaattgt cggataaaac gcttcgtccg cctcagcaaa gccgtaccgg
     4561 cagcgccagc agaaaaaaac cgccgtgaga ccaagtaaca taaaaaagac ggcgtcattt
     4621 tctctcgtgc gtgttttttt cataagcagc gttcccccat gaccatattc tttgtatatt
     4681 ttactctttt tcttggtaag ccgtcaatac gccggttttt caacgtccgg gaacagccgt
     4741 ctcccctttt ttaccgaaaa ttctgtcatc ttttttacac atatttgtat tgacgcgcct
     4801 tttccctcat ggtataaaaa aagcatgaac ctggcgggaa ccgctccggc ggcattccct
     4861 tggcgcaacg atatgaaagg acggaaaaac catgagcaaa gaagagaaga tccccgaaac
     4921 caaagccgaa gagagagctg aagaggacgt cgagaccgaa ttcgtaaagg aaatggccgt
     4981 agagcccgtg gaagttccgg atgtcaccta cgaaggcgag atcgttggct ggacggagca
     5041 tatgccccac catgaaggcg agcaccacga acaccatgag caccacaagg aagaccccga
     5101 ggagctcgtg gagaccgagt tcgtgaagga aatggccgta gaacccgtgg aagttcccga
     5161 tgtcacctac gaaggcgaga tcgtcggctg gacggagcac atgccccacc acgagcatac
     5221 cgaggaaaag aagtaaatcg gaagtaaatc gccatataca gccggccgga ctcacaggcg
     5281 tccggccggt ttttgccttt ttttatttaa gcggggcttt tgcccattcg ctctcgatcc
     5341 ggtgcagcag ctcatcaaag ccgccgttcg gccagacgga gatgtccgcg ccgtcccggt
     5401 tgataaggca gtcggtgaga agaaatacct tcatgccggt cgtttccgcg atcatgtctt
     5461 cctgcgcgtc gttgccgacc atcagacatt cctcgggcga cagcccgagc cgctgcgcgg
     5521 catcccggta gtaatcggga ttcggcttgc agtatgcgga attttcatag gtcgttacaa
     5581 acgcgaaatc ctccggcgcg agccctgccc agcggatgcg ggcgcaggtt gcgatgctcg
     5641 ggaaaatcgg atttgtggcg agcgcggcgc ggtagccgag ccgttggagc tttgcgaccg
     5701 tttccgcggc tttgtcgttc cgagggcaga agacgcttgc ccgttcaaaa tcgtcggcgt
     5761 aaaattcttc aaagaggggc ttatctctct ccgttttctc cgcgccgtag gtcctggaaa
     5821 acatctccca gaaagctgcc tcgttcgtct gcacgccggt atttttcacc atggcggccg
     5881 taccagccca gactgtatcg atcagcgttt tcggctcgta cccgtgggga gcaagcttcg
     5941 ctgcgagaag cttgaaatag cctttcgtaa attcatcctg atccatcggg agcagtgtcc
     6001 cgtccagatc aaacagtatc gccttgatca tctgtgtagc tgccctgctt tcctgctttg
     6061 atttttcgcg gaccgcgctg cctcgccggt tttcagtccg cggcgcttta ttatagcaaa
     6121 atccggcccg cacgtctacc ggggcgatat gcgctgcttg gtttcggttt tttatctaaa
     6181 aaggttgaca caacacgccn nnnnnnnnna attcatattt gtctgattat ctcttattcg
     6241 ccatctgcgc cgggaagacc cggtgttgag aataatggga aaaaatttga aaggatgtgt
     6301 cccctatgat ccgcacttac cagcccaaga agcgccagcg tgccaaggag cacggcttcc
     6361 gcaagagaat gtccaccgcc aacggccgca aggtcctcgc ccgccgccgc gccaagggcc
     6421 gcaaggaact ctccgcctga gcaccctctc gccgggagcg atctcttcct ccggtgaaag
     6481 ggacgtgagc gccaatggag cataccgaga caattaagaa aaattacgag ttccgccgcc
     6541 tctacgccaa gggcaaaagc gcggtcacgc cgacgctcgt cgtgtatacc cgccggataa
     6601 agaccgacgg caaccgcgtg ggcttcaccg tcaccacccg gctcggccac gccgtgacgc
     6661 gaaaccgcgt ccggcggcgt ctgcgggaga tctaccgtct ccacgaagcc gagctgcgcc
     6721 gcggcgcgga gctcgttgtt gtcgcgcgct cgcgcagcgt caccgctgcc tacagcgagc
     6781 tggagcggga ttttctccgt gcctgccgca agctctcact gatgccggag gatactgccc
     6841 agtgaaagcc gttcttctct ggctcatccg attttaccgg aaaaatatct ctccccgaaa
     6901 gcctccctgc tgccggttta tcccgacatg ctcccagtac gcgctggaag ccatcgagaa
     6961 atacggcgct gccaagggag gctggctcgc attcaagcgc atctgccggt gccatccgtt
     7021 ccataccggc gaacacgatt tttatgaccc tgtgccctga acgggcaacg ctgaaagacg
     7081 ggagttacct tctatgatca aaattttcgg ctccgtgctg ctgttcttct acaacatctg
     7141 caacagctac ggcggagcga tccttctctt cggtctcctc gtgaagctca tcatgctgcc
     7201 cttccagatg aagagcaagc acagcatgat gcgcaccacc atgctcacgc cccgcgtgaa
     7261 ggagctggag aagcgttacg ccaccaacaa gcagaagtat caggaagagg tcgccaagct
     7321 ctataaggaa gccaagatca acccgatgtc cggctgcctg tggacgctga tccccttccc
     7381 gatcgtaatc atcctctact ccgtggttcg tcagccgctc gttgcgctga tgaagctcac
     7441 ccaggagaat atcacaacgc tcacggacgt tgtgacccag ctcggctact acaccacccc
     7501 cgccaagacg gacgcctatt cccagatgac catcgccaac gtgctgcacg agcactttgc
     7561 cgatatcgtc tccaaccccg gcgtggcgga gttcgccgac aagcttaaga acatcaactt
     7621 ccacttcctc ggtctcaaca tgatcgagaa gccgtcgctc atgttctgga acactccgga
     7681 atggcagaac ggcctctggt acatcgcgct gctgatgttc ctcatcccgt tcatctccgc
     7741 cggacttacc attctccaga cgatgctctc ccagaagatg aatcccccgc aggatgccca
     7801 gaccgcccag accagcaaga ccatgaacct cgtgatgccg ctgatgagta tttatatctg
     7861 cttcatcatg ccggtgtcca tgggtctgta ctggatcgag cagagcgtgc tcggcatcat
     7921 tcaggaagcc attctcaacc gttactacaa gacgaagctc gacgcggaaa tggccgagtt
     7981 caacgaggcg cagcgcaaga aggacgccga gatggaagcc aagcgcgccg agaccgagcg
     8041 tctcaaagcc gagggcaaga cgcaggtcaa cgccaacacg agcaagaagc gtctcgccgc
     8101 gcaggagcgc aacgccgagg agcagcgtct tgctgcgatc cgcgctgccg agcgcgccgc
     8161 gaagaatccg ggcgcggagc tgcccgcctc gcaggtcggc acccgccgct ttgcccgcgg
     8221 ccgtgcctat gtggcggacc gctatgacgt gaccgaggcg gaagccgccg ccgagaccgc
     8281 cgaagccgct gccgcgggcg aggccgatct cgaagaggcg aaggcacagc tcgccgaaac
     8341 ggaagctgtc gagactgtcg aaacggtcga gaccgccgag gaaacggccg aagctcccgc
     8401 cgccgaagag aaggagtaaa acacacatga ttcgatccat agagaccacc ggcaaaacga
     8461 aggagctggc catccgcgcc gctctggagc agctcaagct caccgaggac gacatcacct
     8521 atgagatcct cgagctgggc cgcagcgggt ttctgggcat cggcgccgtt ccggcgcgcg
     8581 tccgcgtcag ctatgacgac gggcagagcg agcccaagcc ggtgaagacc cagccggtca
     8641 aagccccggc gaaaaagccc gaaaccgccc aaaagagcga gccgaagaag gccgagcctc
     8701 gtcagtacgc cgccgggagc caggaaaagg aagtcgagag cttcctccgc ggcctgctcg
     8761 agcgcatggg cgtggaatgc accatcgaca tttccgaacg cgaaggcggc gggctggaag
     8821 tcaagctcga cggccccgat atgggcgccg tgatcggccg ccgcggtgaa acgctcgacg
     8881 ccatccagca ccttgtgaac tatgccgtca accgcggcag cgacaagcgg atgcacatca
     8941 gcgtggacgc cgagggctac cgcagcaagc gcgaggaatc gctcgtccat ctggcgcaca
     9001 agatggcggc gaaggcgatc aagtataagc gcagcatggc tctcgagccg atgaactcct
     9061 acgagcgcca tgtgatccac acggcgctgc aggactacga gggcgtttcc accagctcca
     9121 ccggcgtgga gccgaaccgc cgtgtcgttg tcaactatga gcgccccgcc ggagagacgg
     9181 aggagccgcg ccgcggcaat cgcggcgagc gcagccgccg tccgcgcgaa aaccgggaaa
     9241 accgcgcccc gcgcgcggcc gctcccgcgc cggagaagaa ggcggagacc gccgaaccga
     9301 tccccgcgcc cgagtatccc gcggactaca ccccgccggt gaagaacaag ccccagagcc
     9361 gcgagtggtg ctgatacatc aaaaaggagc cttgctttgc aaggctcctt tttgagaaaa
     9421 tgcggttcgc cgcagcgcac tcgctcggct cgcacgtttg cgagccgagt ttgccatcga
//
LOCUS       DAWAAE010000076         9456 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3276_length_9456_cov_2.798426, whole genome shotgun sequence.
ACCESSION   DAWAAE010000076 DAWAAE010000000
VERSION     DAWAAE010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3276_length_9456_cov_2.798426"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6376..6465
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1776 a         2619 c         2887 g         2084 t
ORIGIN      
        1 gttcagcacg gtggagccgt cgcctttgac gttgacggtt tcggccttgt ccgcgttgta
       61 ggtgaaatgc ttatcgtcgc ggccttcaaa gctctggttc tgataatcgc cgctgctctt
      121 ctgcgtaccg ggtgcaacat cggcgggcgt gaacgttcca gctacgctgt agccgacgtc
      181 attggcgttc tcataccaga atacgacggt aaatccggca gtgccctttt cccagtttgc
      241 cgtgaagctg agaggattct caataaccgg cattgttttc ggaatctctc cgctccaacc
      301 agtaaatgta tagccgggct tttgcggatt tcctacattg accggcgcgc catagcgggc
      361 atagatgggt tccacgccgt agccgccgtc cagattgaag ttgatcagat aataatagcg
      421 atcatacttg acttcgacta ccgtgctttc gtcgccggcg atttttaccg tagcgtcata
      481 cttcagagaa taaaagccgg gatactgctt ttccagcgtt tcgccgactt cgtctccggt
      541 gttaccgttc ctgatctccg gatcgccgtc ctgcgtatat tcatcgttgg aaacgttctg
      601 gagaagatgc ttaacgctga actttacaac ggcgggcgta tatttaaccg tgaaattctg
      661 atccgcctga atgttctcac cgccaacggt gatttccgcc tgttcagccg tatatcccac
      721 taccacaggg ctcttaacga cgctctggta ggtgccgcca gccataaccg tcgccgtcca
      781 agggtcagca acctggtgat ccttattatc aacatagttg atggtaatgg tgaaagtctg
      841 ggtatagggt tcctcgccgc cgtcgtttac cggggcgggg tcgtttaccg gggcggggtc
      901 ggtgagagtg gccggggcga cattcgccgt caggtcattt gccggagcag cgtccggagc
      961 cggggtctct tccacggtct gcgggggaat aatgaccggc tcgcctaccg gctcggcctc
     1021 ggagatgacg gtggggacct ttggagcctg cgccggagcg gagacgggcg tttcgtccgt
     1081 gatctgcacc gtgacggtgt tggagtatac cggctcttcg gctgcgccgg tgacgcggca
     1141 gcggatgttc gccgtgccgc cgtacagcag gttcgccaca agtgcgtagc caacttccaa
     1201 ctccgcgccg gtagcaccgg caatattcgc ccagacgtcg ccgccgacct ggatctgcca
     1261 ctgatagtag ccggacaggc cgcccgcagc gagcgtcctc ttttcatttt taggaacgga
     1321 aactgtatcc acagcgacat tctcgctgta gatcataagc tccgagtccg cagcggcggg
     1381 ggtgatgagg gcggcggtca cgaggccgaa ggaaagagcg cagcagagaa acagcgctaa
     1441 cagcttcttt ttcatttcac gacctcctta aggatttccc ggaacggctg tcggaacttg
     1501 tgtcggctcc ggcacctgcg tcggctccgg cggagcaaag ctcggccagc cggcggcgga
     1561 gaacacgtcc gtgccgttgt tgtccgcctg caccgcgccc acttccactt ccacatacgc
     1621 cgtagagttc tggtactcgt tgctcatctc cggggcaaac gttaccgtgg tgaagagcgg
     1681 gacggtcgtc tcgccggggg cgagagcgcg gttataatag tagtacccgt ccagatacgt
     1741 ccagtccgtt tcgctgaagt cgagcgagac gtactcgggg ggcagcgtct cttcgtcggc
     1801 gccgtagaca tcgagctgca cctgcgcacg cacccagacc ggggcggtgc cggtgttgct
     1861 gatctcaaca atcttcgtca ctttcgtgcc gggcatgacg ccggtgcgat cctcgaacgg
     1921 ctcggtgcgg tcctcgttcg cccattcgtt gagcacaatg tccacaccac cgctggtgat
     1981 aacattgtgc gccttggtgt ctgcggtgaa gtatgccagc gtgttcgccg caagtatggc
     2041 gatcaggatg gcaagcaccg acagaatcaa gaattttcgc ttcattgctt ttactcccct
     2101 caggctgtat ttgcaattat ttcttgcgct tttcatggct cttcagacca taggagaaca
     2161 tctgcatttg cattagatgc tctcctatgg tctgcaccca cgggggtgca gaccatattc
     2221 gactgggtat gtggagggat gtctctccct ccgtcaagcc cttcgggctt gccacctccc
     2281 tcgtcagagg gaggcttttt tcattggccc ccttgtgcaa agggggctgt cagccgttcc
     2341 ggctgactga gggattgttc cttcgtcttg ccgatacgga caatccctcc ggcgcttcgc
     2401 gccacctccc tttacacaag ggaggcttta cggggaatta ctgccagttg gaagcagcgc
     2461 cgcgccacct cccctttcag gggaggcaag atttgggcgg gagattactg cgcgtcaaac
     2521 gcagcccaag cggcagtagc gttggcaaag ccgtcagcct ggatggcgtc ggcctcgacc
     2581 agaacgttca gagccataac ctgagcgccg tcggtcccga caaccgtgat ggcaccggcg
     2641 tcaagggcct tctggatatc ggcgctggta gcagtcttgt cgacgccgat gaagttccag
     2701 acgttccact cggtcatagc gccagccttc aggggctcgg tgcgggtgaa ggtgtacacg
     2761 tcatacgtta cgccgttctt cacaacaccc ttttcaataa caggccagcc agtctgtgcg
     2821 ccgctctgga attcgccgca gagcatggac gtggtgcacc actggttggt gatcacgccg
     2881 ccgtccttca cgtcaacaaa gtcattgttg gccgcagagg ggaccatcac gcggatacgg
     2941 atgtaagcgt catttgcgcc ggtgttgacg acataggggc acttggcaac gccgcggccg
     3001 ggaaccatgt tctcgccctt cacggccaga tagtccgcca tataggtctc agcgtctgcc
     3061 ttgatctcgg catcggtgaa agccttatga ccgtcggtga catacctcat gccggacgcg
     3121 gtctgggtgg ggtcggggat ggaggtgtcg ccggagttat cgttgccttc gcggtggaac
     3181 ttggactcga tcagatcgat cttcacgttg ccgacggtga aggtattggt ggcgttatcg
     3241 gtgtcggtga agtaagcaag agaggcgccc acaacggcga ccgccagaag ggcgacgcag
     3301 aggcacagcg cggtaatctt cttcttcatg tacttggtac tccttcttta agtaattaag
     3361 ggtcgctatt gcttagggat tagtaggctg agggattttc gaccacgcag tagccgcgtc
     3421 ggcgatgttc tcctgctgca cggcgtaggc cgtgatcttc agggtggggt tcgccgtcgg
     3481 gatgttctcc ttggtcaggt cagagctgac agtgatctta ttgcctttga taacctcaaa
     3541 agaagtatca gcggtcacgg cgtcaaccag acggtagtaa acgccgctct ccagctccgt
     3601 ccagccgtca gcgatctcgt aagacatgcc cgtaaacata cctaccttct cgaccttgac
     3661 gaacaggtag caggcctcgg agccagcctt aacggtgacg gtcgggtcct tcttgatggt
     3721 cgtgccgggg atgaccttgt aggacgtacc ggtggactca gccagagcga tgttgatatc
     3781 gccgtaggta aaggtgttgg tcacggtatc ggacgtggcg acgagccagg cgacagtacc
     3841 tcctgccaca cagccaatca caagaacaag cgccagaaga gcgataaacg cgcggctgct
     3901 cacactgcgg cgacggtttg caacatgctt cattttcatt cttcctcctt tcttccgtta
     3961 ttgacaggga aatctgttat ttccatggat aacgattatc cttttccctt tggtttatgc
     4021 gcggggtgtc agggctgcgg cgcaaaagcc gcccacgcag caccggcgtt ggcaaagctg
     4081 ccggcctgaa tggcttcggc aacgaccgtg atctggaagt tgttgagctt ggcaagctgc
     4141 tcgccggtag cccattcggg aacggtgatg ttgttgaaca ggacggggag cttcacgtcg
     4201 ccattggaag cgccgacggt ctcgttgtag tagaagtagt aggtcagggt gtcagccgtc
     4261 ttgtcatacg cgaagtactt ggcgttgccg agcatatctt cggtatcgac catattgtca
     4321 ggaatgatgc cgtcccactt ggcggcgttc gcttccacga acttcaccat gcggatcagc
     4381 gggtaagcgt tctcaacgcc ggtggggccg tcgtccgcaa actccggatc ggtgcagagg
     4441 gcgatgatct ccgtcgcgtt gttgaaggtg accttcatgc ggacgtagga ttcctcgctg
     4501 ccagccttga cggtgacggt cgggtccttg gtgtatgtag tgccgggcat cagcttgtag
     4561 ctgtttgcct tcacgcgggg gccgttcggg ttggtgacgt cggtctcatc gagcgtgatc
     4621 tcgaccttgc cgatggtgaa ggtgttggtc acggtgtcag tcgaggtcag gtacgccatc
     4681 gtgcccagca cggacgccgc gacgagcaac accgcacaga gagtcaggag cagagctttg
     4741 cttttcgttt tcatgttgcg aaaacctcct aaattaatgc tttctatagg gagccttgcg
     4801 gcgttgccct catcttttgc ggacgtgacg gcccgcggcg ggattggccc gttttttttc
     4861 gtcctccccg acggtctcgc tcttgctgtc cggggcgaag atgtccggca ggaacgtcag
     4921 catcaggagc acggctccgg ccgagatggc cacatacatg ccggggggat gctggatgta
     4981 gtttgcgacg aagccgaggt acgggatggt gaacaccggc gtgccgatga cgttcttata
     5041 atgcacaaga ccgccgtctt cggtttcgtt cgcgtcgccc ttcgtgcgga agcggacgac
     5101 ggtggagtcc tcctcgtccg gcacgacgcc gacgacgcgg tgcgtggcga tggtgttttc
     5161 atcgagcatg aaggtgatga cctgtccgtc ccgaatggtg tacgggtcca cctttttcac
     5221 atagatcagc gagccggtgt ggtacgtcgg ctccatggag ccggagagca cggtgtacac
     5281 ctgcagaccg aagagccttg cgccgacgag aagcagcgcc agcagcacca cgagcgccac
     5341 cagaatgctg ctgatgacat tccacgtttt tcggatcgag cttttcttca taacagcgct
     5401 gtttcctttc ccgctccctc tgtcagttcg gcacggaagt ggggcgcgat aaagctgtag
     5461 atttgctcga aggtccgttc cgttgtaagg tcgttgtttt ccatatcgcc cgccatgacg
     5521 tgcgcggcaa aggcgagcat ggtttcaaac acctgatgca tgagcatatc cagatccgtc
     5581 tcggggcgga aaaggtatgc gacgcggtgg atcagcgcgt ggtagtattg ctgctgccgg
     5641 tcgtgctcgt aagcgtagaa gtcgggggag ctgtaatacc ggagatagaa gatgcagtcg
     5701 tcccgcttgt ccagaatgaa tttccacgtc ttttcccaga gatggaagca gcgatcccgc
     5761 cacgtctcta tcatgcagcg cttcggcaag aagctcgtcc ttccccttga agcatttata
     5821 gatatatgct tcgttgaggc cggcatcggc ggcaatggcc ttggtcgttg cctttacaag
     5881 gcccttttgg gcgatgacac ggatggcgct tcggatgaat gtctgttgaa tttctctctg
     5941 ttccaaaatg ggtactcctc tgcaggttct gcgatcgcaa aaataagtgg ttacttacgg
     6001 tgagggggaa aaacagcaaa tccgcccccg ttttgctaaa gaacgggacg atttgctaac
     6061 aaaacggcga attcttatca tggagttcgc ccaacctatg atatgggaag cttttcacta
     6121 tgcagcgtaa gtatagcata ggtaaaataa aaaaagcaag actttcaccg gattttttaa
     6181 tcattactcc ggttaaattt tttgttgctt cttaacagct gtaagtttct ttacatgtgt
     6241 atatattact ggcatgtatt ccagtatttc cgggcagtca aactggcagt caggccattc
     6301 cggcattttt tgccgcagtc aatttactat attataataa gaaggaggca tgaaatgtgt
     6361 cgaagggcga aaacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacgc gaaaacgccc
     6481 ggaaacgccg ggtttttcaa ccggcgccgg gcgtttttga cagaggggcg aaatccgcga
     6541 tatgcggaga gcgcggaatg ggcaggtttt ccgcgggctt gctttgggcg gagcagagcc
     6601 ccgcccatcg gggtatatcg ggtgctcctg tatgggcgga gcagagtccc gcccatacgg
     6661 ggtgcatttg gcactccgcc ctttttatcc gagggcaagc ggcttgccgt ccagctcggc
     6721 ggcggtcagc tcttcgccgc ggtagacgag cttgagcgag aaaaacggcg cgtcctgcgc
     6781 gagcaggcga agaaaaattc tgtcgcccgc ccagagcttt tttgtaagca gctcgtcctt
     6841 gccgatccag gcaagctcgc cctcgtcgca ggggcgctgc tcgcccgtcc acgcgtccgc
     6901 ggtgaacagg tgcatatatt cgcacggcca cacgtccgag acgaacgtca caaggccgcg
     6961 atagcgccag cgcgtgagcg tgagccccgt ttcctcgcgc acctcgcggc ggagacagtc
     7021 ctccgggctc tcccccggct cgaacttgcc gccgacgccg atccatttat cgcggttctc
     7081 gtcgttttcc tttttcgtgc ggtggagcat cagatactcg ccgccgcgct ccatatagca
     7141 gagcgttgtg agtttcgttt ccatttataa tacttccccg ggaaattccg tccacgggta
     7201 cgccgcgggc ggcttttttt caaatgtcag catctcgccg gtgagcgggt gcggaaaggc
     7261 gagagcgcag gaaaaaagcg cgatattccc ttccccgctg ccgccgtatt ttgcgtcgcc
     7321 ccagagcggc gcgccgcgcg cggcgagctg acagcggatc tggtgcgcgc ggcccgtgta
     7381 cagccggacg cggcagagcg agagcccggc gcgagaggcg agcacttcat aggcaaggcg
     7441 cgcttcgcgc acgtccttcc ccggcgcttc ggcgagaaac gttttgcgca gtctccggtc
     7501 gcgccgcagc agatcgcgca gctcgccgcg ctgctccggc agcgcgccgc aggcgacggc
     7561 gagatattcc ttgcgcacat cgccgcgctc gatcgcgcgg ccaaggtcgg cggcggcgtg
     7621 gcgcgtgcgg gcgtagacca ttacgccgga aacggcggcg tccagccggt gcacggtgta
     7681 gaccggggcg ctgtcgtccc cgagcgcggc gcgcagaagg gacggcatgc cgcccggctc
     7741 gtccgtggag aggacgcccg cgggcttcac gcacacgatg atcttgttgt ccagatacaa
     7801 tacttccaca gtgacgctcc ttttgaacac tatatattgt atcacatacg gggtagcgga
     7861 gcaaggcatt ttgtggtata cttctccggt aaatgcaagg aggaaaccgg catgacggaa
     7921 atgtatcccg cgtattatga ggcgtttcgc tgcacggcgg gcgcgtgccg ccacaactgc
     7981 tgcatcggct gggagatcga cattgacggg aagagcgcgg aacggtacgc cgcgctgccc
     8041 gaagccttcg gcgagcgcgt gcgcgcggcc gtcgagccgg gcgagccgcc gcacttccgg
     8101 ctggacgcgg cggggcggtg cgccatgctc cgggaagata atctctgcga attgcagcgt
     8161 acgcttggcg agggcgcgct ctgcaatatc tgccgcgatc atccgcgcta ccgggtgttt
     8221 ctgcccgggc ggacggagat cgggctgggg ctctgctgcg aggaggcggt gcggctgctg
     8281 ctttcccgga cggaaaaagt gacgcttcgc ggcgcggtat cgaaagcgcc gccgcgtgag
     8341 accgtggagc tgctgctctt ccggcagaag ctgctggacg ccgcgcagga ccggcggctc
     8401 actctcggcg gacggatgcg aaaaattctc acgctcgccg gcgcttccct gccccggcgg
     8461 acggcgcgcg cgtgggcggc gtttttcgct tctctcgaac cgctggagcc gggatggacg
     8521 gaagcgctcg caaagctggc ggggccggtc gattttgccg ggttccacgc cgtttccgcc
     8581 gcgtgggaga cggaatatga gcagctgctc gtctatttcc tgctgcggca cctgcttgca
     8641 gacgaggggc gcgcgctgcc ggcacgcgcg gcatttgccg ttttgagcac cgccatgctc
     8701 tacgcgctcg gcgcagccgt acggacggaa aacggcacgg tcagctttgg agagcgcgtg
     8761 gagcttgcgc ggctgtggtc ggcgaatgtg gagtattccg aggaaaatat gcgggcggcg
     8821 cttgatgagt tgacgtaatt ttatttacat aatatagttt tcataatgtt gcggagggct
     8881 tttcattgga tatctgggaa aggctgtatg aggcggcgtc gaaagagtat catccggaag
     8941 aggtttcacc gttcgtgtac gcgcaccacg tcgtatgcgc gctggaagcg gacaacggcg
     9001 aaatttatac aggattctgc attgaagcgt gcagcggcgt gatgaatctc tgcgccgagc
     9061 gcgtcgcggg gctgaacatg tacatgcaga gcgggcagac gaaggtgcgg cgaatgatcg
     9121 cctttcggga caggccgccc tgcggcgagg gcagcgggat gccgtgcggc gcgtgccggg
     9181 agttcttcta tcagctcaac gaggaaaacg aggatatgga gatcctagtc gattacgcaa
     9241 agcgcgagac cgtgacgctc cgcgagctcc tgcccaagtg gtggggtgca gaaaggtatg
     9301 agcaggccag ataacgtcac ggtgaaaccc ctcagtcggc tgcgccgaca gctcccctgt
     9361 taggggggcc gagcttgtca aaaagccttg cagagtttgc gcccgcaggc gcaaatagag
     9421 ttttaaacat tttctccggc ggcgtgtacg tcggag
//
LOCUS       DAWAAE010000077         9438 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3291_length_9438_cov_3.537355, whole genome shotgun sequence.
ACCESSION   DAWAAE010000077 DAWAAE010000000
VERSION     DAWAAE010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_9438_cov_3.537355"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1610 a         3020 c         3055 g         1753 t
ORIGIN      
        1 agaatgtgac gtactgcgtc acatcgcgct caaagccgtt tttgtaaacc gccatgatct
       61 tcatgccggc cggatcgaac gtctccccgg cgcggtaggc gagcttgtcg ggctgcccgt
      121 ccacgcgaag ctccgtgatg acgctgccga gcgccatcat atacgccgag tcgttcttga
      181 agtagatcgt gccggaggcg tcggtgatcg ggctgcagat ggcatactgc gcctgatttc
      241 cgtcgggcgt gaagagcacc cccgcggtct cgtaggagat ttccttcccg ccctcgatcg
      301 ccgtttccgt cacggtgatg tcggcgcgcg tctggccggg cttgtcgcgg agaacgcgca
      361 gcttgccggg cgtgtagtta tcgaagaagt acacatacac cgtgccgtcg ccctcgttat
      421 aggcggtggt gagaaggccg ctcgtctggg ggtagccctg cgtgcgcacc gtgtaggcga
      481 tcttccatct gttgagatcg atcacggtga tgttgtggcc ggaatatgcc ttgaactggc
      541 cgacgccgga gatgccgatg tatgcgcggc cattatagat gaccggggtg cacgtgctca
      601 tagcagggtt cttcgcgtcg ttggcgtagt tatagagctt gatgtgccgg agatcgctga
      661 gctcgccgtt ttcggacacg gcagcgctgt agaagtagcc gcctttcgtt gtgaagtacg
      721 cgcggccgtt atcgtaggtc acgctgctgc gcgcgtcgcc cggatgcgga agccggatcg
      781 agctgatgag ctcgccggtt cgggggttga gcgagagaat gctcgcgtag ccgctcgtat
      841 agccggaatc gccgtcgtcc gtcgggatga ggacatagtt gtccgccaca tacgcgcccg
      901 cccagtagaa gccgccgaga ctcctgtact gccaggtggg gagcttttcc tcgtcgcccc
      961 ttgtcgggtc ttcatccgtc acggacaggc acacgaagtt tgcgttttcc ttttcgccct
     1021 gccagaagcc ggtgtagata tagccgttat ggtagtagat cgggcagttc ggctggccgc
     1081 cgagtgagtc ccggtagatc cagagcgatt cgagcgtatc agcgtcgaac gcctgcacgc
     1141 cgccgtccga gagtccgaca aagagcatgc cctcggcata cgtcgggctg ttgatggcaa
     1201 aggacgatct gtgatccatc ttccccttgc tctcgatctc gccggtcacg acgttcagct
     1261 tatagattat gtccttggcg taggtataga gatgatcgcc cacaaggatc gggcagccgc
     1321 aggcgtcggc gctgtagttc tcgccgagct gcgtcgccca gtagagcacc gcgtcctccg
     1381 ccgtggtggg cgttttagca ttcaccacgc cgttattata ttcgtccgcg cggaacgacg
     1441 gccagacctc ggccatcggc tcatacgagc gctccggcgc tttgtcgagc gtcacagtga
     1501 ctgtctccgc cgtatccggc acagtgtagg acatttcctg tacgccggca tacccgttcc
     1561 gggtcacggt gtagcggaag ctcgctcccg gcggaagcat cgccgtgccg gtatcgtcgg
     1621 tatagacgca gctgccggta tcgttgttca cgaggaacac gttggcatcg gacggcgtca
     1681 cggcaaacgt gatcgccacc ggctgcttct tgacgaagtg gaggttatag accgtggaaa
     1741 cgacgttcgc atcgctgtgc agcactttga cggcgatggt ctcccgtgcc ttgtcgggat
     1801 tgagcgccac agtcacgccc tggatatctg cgcaccgctc gccgccgatg tccgcagcgt
     1861 agccgcccga ggcggcgtac tgcgccgtca ccgtcgccga cgcggtcgtg ttggcgacct
     1921 gcatgtaata gtccgttaca ttgccgttga aggtgatcct gttgctatcg gcgtcggtaa
     1981 acgagagtgt ttccccgtcg gcggcgaccg taaggctctt gagatgcagg ctgcggacaa
     2041 ggaagagcgt gtagtcctga tagtaagtgg taccgccgct cgttttggac gcgcgcagcg
     2101 tcagcgtgcg ggcggtgccg ccggtctgga taaggttgtt gagccgcgtt gcactgctgc
     2161 cgccgacggc ggcggtgacg ggcgtttccc tgatgccgtt gacaaggccg gtggaatttt
     2221 ccacgttgtc gtggatggcc gtcacggtat agccgctggc tgcggttgcc tgggcgtaca
     2281 tgcggctgat cgtgtccgcg atcacatagc gcacgctgtg cgtaccggcc gcgaacggcg
     2341 tttccggctt gaactcgttc cgggtggagg aagacaaatt gtcaaagagt gccgcggccg
     2401 tgatcgcgtc cgccgttatg ggcgaggccg ctttcacggt gagaccgtcc gaagccgtaa
     2461 acacagcgga ggcgtggtac cacacatcct tcgtcgtgac gcaggtatag gagcctcccg
     2521 ggatgagtgc atagctcccg tccgctgaag cgggaatggc gttgtccgcc gcgtcgcgca
     2581 cctccgcggt gcagccatcc ggcacggtga gcttaacatt tgccgccgtg cggcgcgtga
     2641 tatgcagcgt atacttcgtc gtctggccgt tcgcggaaac ggagatctcc gcgtcggtac
     2701 ccgccgccgt ctggactttg ccggtgccgg agacggtgta gctctcgccg tagccctcgg
     2761 cagcgagaag gatgctgtcc gtcacggcgg tcagggtata ttcgttcgta taggggtcaa
     2821 aaaccggctc gacgatctgc ggcgttccgt ccggcgcttt cacggtgacg gacttaagcg
     2881 tcggtatgcg cacgatgtgc atcgtgtacg tctgcacctg catgtagccg tccgcgcccg
     2941 tatagcgtcc ctcgagcggc acggtgatgc cgtccatgcc gaagccgatg cactgcacca
     3001 tgctcgtata gatcgaatcc caggagcgcg tcgtttccga ggcatccttc ccggtgtcgg
     3061 tataggcgta taccgtgcac agcttggtgt ttttctcatc cgggacatca cccatctgcg
     3121 cgttgagata aaggaacgtc gtgcagtccg gcacatagaa tgtcccctca ttcgttccgg
     3181 cgtacacgga gcggtagatc tccttatcga acgccgggga gccgaaattc cggctcaggc
     3241 gcacggcgtc aaaccactgg ccgtgcggca gcgtcacggc caccgccccg gcggggcttc
     3301 cctcgccggc ggtgagcgtg ccgcggacgc tgttgcagcc gccgtccgtc acctcaaagc
     3361 tgtatttccc agcgataacg gagatggacg cgccgtccgc ttccgtcacg ttgccgtagc
     3421 tgtcggtcat gcggatatgg gatttggccg gagacgcgcc gttctgcgtg ggcgcaaacg
     3481 tcacggtgta ctttgtcccg gcaagcagcg cctcgtattc cttcacggcg gcgttgagtc
     3541 cggcaagaag tgcgtcggcc tcctccgcgg aggcggtgcg gaggcccgtg agcgcggcgt
     3601 cgtatgcctc ggccgcggga ccgtagctct gcacattgtt cgtcatttcg gcgaacttgc
     3661 cgagaccgag gatgagcgcg gtcacggagg cgctgttctg ccccatcgtc tccgtaaagg
     3721 ccagaacgtt gatcttctcc gcaggaacgg tcagatcata gtcgttatcg ccgatcgtat
     3781 agtcgtggaa gcgcacgaaa tttgcgctga ccccttcgat cgcctcgacg gatgccttgt
     3841 ccgcgccctg aaatttgaaa tcgaacccgc tcgattcgat ggcgtcgagg accgactgcc
     3901 cggcggtata cgtcacacgc gtcggggcga tgaccgtttt ctggttcgtc acaacgctca
     3961 gatagaaata ccccggctgc gaatccgtcg ccgccgccgg taccggcagc gccgacagca
     4021 tcaggcagag cgcgagcagc agcgccgcga tccgtttgct tcccatcagt ttcttcattt
     4081 ggtccttttc tccttttaca gatacaggca ttcgatccag cggtatacga gcttctgtcc
     4141 gaagctccag cggtgccggc acgcatcgag caccgtgcgg acatatttct tcatggcatc
     4201 gcgctgcgcg tccgtcatgg cgtgcgcgct gaagagcgcc tcgcggttga ggtctgccgc
     4261 ctccgccccg ccgtcaagct cgtttggcgc gagactcgtg cggcgcagca gcgccgcggc
     4321 gtagccgtag agcgcggcga tcgcgccgcg gttatccagc cgctcgatcc tttgcagcgc
     4381 cgcgcgcagc ttccgccgcc gcagcagcac gaagagcgcc gcggccagca gcacgagcag
     4441 cggcagaagg atcgccgtta catagctcgg cacgcggaag tgacggagca tgccgccgtt
     4501 ttcggtgata tcctcgggcg tgttgtcctc tggcggctcg ggctcggtct ggctgtagta
     4561 gcgctcggtc ttgaggccgg tgccgggacc ggcgttgagc tcctcgtcat cggtgtagcc
     4621 gggcgtcgtt tcaaacggga tccagccgac accgtcgaga taatactccg cccaggcgtg
     4681 agcgtttctc tccgtgagaa cgatccgctc cccggcggca tagcgcccgg cttcctcggc
     4741 ggagaggaaa tacccctcga cataccgcgc cggaacaccg aagtatcgga gcatgagcgc
     4801 cgcggcggtc gcgtaatgca cactgtagcc gctgccggag cgttcgagcg cataggtgag
     4861 aaagtcggtg tcaccggtgt agctcacgac gctctcgctg taggtcagac agctctcaag
     4921 cgtttgcagg atgatgtcct tgatctgcga gagcgtgcgc ttctccgcag ggagatcgag
     4981 cgcgcggccg atggccgccg ccgcctcgtc cggcagcgcg agatagttcg cttcggcata
     5041 cgcgccgtag tccgcttcca gttcgaggaa cgcctgcacg tccgccccgc gctggcgcac
     5101 ggagagcgcg gactgcgcgg tgaaccagtc cggcacgctg ccggggatat acgagagcgt
     5161 ttcctctgcc gtgcccggcg cggacgcatc gccaatgagc gaatcgtcca gcccctccca
     5221 tgcgcacagc gcatacggga gataggctcg ctcccggcag gccgagaggt tttccacgat
     5281 catgtccgcc gccggggtac cggcggcgag ctccgccgcg gcggacacag aagtgagcgc
     5341 gttgaacccg tcggcgtgca gccagtagaa atcgtccgcc cacgcagccc tctccgcagc
     5401 agggagagct tcccaggccg tgccggtgta tacctcgccg gtcatgccgc gaagatagag
     5461 cttctgcgtc gtgtccatcg tgacggcgag cgccggggta tcgtttttct tccacgcgcc
     5521 gagatctttg agctgtccct ccggaaggga gtttgtgttt tcatcgtagc gcagcgcgtg
     5581 ccatgcaagc gcagcgtcgg tctgcgccgc ggcaggcgcg ccgccgagcg cgaatacccc
     5641 cgccgcggcg agagccgcgc agaggaccgc ggagacgagt gagagagaaa acctctcccc
     5701 gccgctgccg cgcagcagcc agagcacgag cccggtgaga aacgccgccg cgccccagcc
     5761 cgccgggaca atacccgccg cagcgccgag aataaggagc gctgcgacga tcagcgacgg
     5821 caccgcgccg gtgcagagca gaagctccgt cgccgcagcg agaaagagcg ccgcggcgcg
     5881 cgcgtttgcg gtgttttcca cgtcccagag catatggatc cgtccggtct tttccggcag
     5941 gacggagagg atgtcgttgc cgagcgcgca gaagccgtcg cgcagcgggg caaagaaaat
     6001 cgccgccgca gcgccgagga cgagcacgcc cgcggcgagc atccacggcg cggccttcgt
     6061 ctgcgccaga gcgagcagtg cgggcgagaa ggcaaagagg atcgccgcag ccgcccagcc
     6121 gttactcacg ccggacggga acaccgacgc cgccgtctgc gcgagaccga gcgagagaag
     6181 cgccgccggg agcagcgcgg aaaggaacgc ctttccccgc ggcggcgcgg cggagcgctg
     6241 cgcgcggaga acaaggccgg tcttatgttt ctttttcatt ccataaactc cagatcacgc
     6301 agacgctgcg catagtcctc cggcgtgaac cgaacggtcc ggcacggcga ggccgtctcc
     6361 gcacgtgagc agagaaggag cgtcagcgcg caccgctcgg ccagcgcttc gatctccggc
     6421 ggcacggcgt gcgcgaagag cagcacgcgg ccgtaaaaat tctcgccgcc ctgccggacg
     6481 agctgagccg ttccgctctc ccccgctccg gcggagcgca ccatgcgcgg cagcagcgcg
     6541 agcagccggt cggtatccgg cacttcttcg agaacgttct gtccttcgcc gctccagccg
     6601 agcgtatagg caaagcccgc ctcgctcagg ctctggcaga cggaggaaac gctctcggca
     6661 agcgcgtctg cagcatccga cgcgagttta tccgccgctg tcttgtccca gaacaccagc
     6721 agcgagcgct ggagcgggag actggcctcg cggacgatga gccggtcggt cttggctgtc
     6781 atcttccagt ggatgcggcg cagatcgtcg ccgggggcgt attcgcgcag ctggaagatc
     6841 tccgtgagat cgttcccgcg gcagccgggc gcgtagctgt cgctctcctc cggcatggag
     6901 gaggcgagcg caagatcgat cccgggcgcg aacatgtccg gcagggcggt cgtgcgcgcg
     6961 gcgatgtttt ccgttttcaa tggtacggca aaaatgccgc atatatcgag aaggcctgcc
     7021 ttttccaccg ccgcggtgat ctgaccgcag cggcggctct ccacgagaaa gcgcagctcc
     7081 tccgtgccct tcggcggaac ggtgcagcgc agcagcgtga ccgtttcctc gcgtgtgagc
     7141 gcgttcaccg cgcgcacgcg gaagagcgcg cgcgcactca gcagcgcgtc atttttcacg
     7201 aggagcaccg cctgcgccgc ggcgcgcttc ggcaccgtgg gcgtgagcgt gagcgagagg
     7261 cgcacgcccc gccgcgccgc aagagcgagc agaagcgata ctcccggcag aagcagcaca
     7321 gcgatcgccg cgcccagcca gagcacgccg cctccggcgg cccagaccgc cgcaagtgcc
     7381 gccgcagcca gaagccacag cacggcggcg gtgattcggc gtgctttcac ttccggaact
     7441 cctgcacatc cggctttttc acctcaccga tcatgctctc gatgagctgc tcggcggtat
     7501 attcgttcaa acgggccttg gcgttgagga tgagacggtg gccgcacacg aacgggaaca
     7561 cctccgccac atcgtccggg atcacatagt cgcgcccggc aaggtaagcg ttggccttgg
     7621 ccgcgcggca gaccgccagc gcaccgcgtg ggctgacgcc gagcgtcacg aacgcgttgg
     7681 agcgcgtctt ttccacgaga cgcgcggcat aatcgtacac gctgtcggca aagtggacgt
     7741 tcgtcacctc gtcgatgagc gcgaggaggc ccgcgatatc cgtgacggct tccaccttgt
     7801 cgagcgggtt ttcatggtga cgctcgcgca ggatctccgt ctggctcttg agatcaggat
     7861 agcccatcga gagccggata aggaaacggt cgagctgaga gttcggcagc atcatcgtac
     7921 cggcggagcc gacggggttc tgcgtagcga gcacgatgaa cggcgtggga agcgcgtgcg
     7981 tcacgccgtc cacggtgacg tttttctctt ccatcgcctc gagcagcgcc gactgcgtct
     8041 tgctcgacgt gcggttgatc tcgtcggcga gcagcagatt cgtcatcacc gcgccgggct
     8101 tataggagag cttcccggta tccttgtcgt ataccgaaaa gccgatgatg tccgagggca
     8161 tcgtatcgga ggtgaactgc acgcgcgcgg tcttgagccc gagcgttttg gcaaaggcca
     8221 tggcaagcgt cgttttgccg acgccgggca cgtcctccag aaggacatgg ccctgcgcga
     8281 gaatggccat cagcacgcgg cggacgacgt cgtccttgcc gacgataact ttctttattt
     8341 ctgtctggat ctttccggtc agttcgttca tgctctcttc tcctttttct tccggcacat
     8401 gactacaata acaacaacgg ccaccgcagc ggcggcaagg caaagtataa cggtcagcca
     8461 cggcgtcccc gccggggctt tttccggcgc ggtctccact gcggtctgcg gcacggcggt
     8521 catatcgtac agccgccccg caccgctccg gagcttccag agcgccgtga acgcgagcat
     8581 gacctgctcg gtcgccatga aatcgccccg ctcatcctcc ggcgtgtggg catagcttcc
     8641 gtccgcgcgg cggtaggtat tgcagagcgc ttcgagaacg ttcgttccgt ttttcgcaaa
     8701 gcgctcgtcc tccgccgggt cgatgccgag cgagcagagc gcaatgacga cctgcgcgga
     8761 gctctccgcg ctcggctggc cccagtaggc gaaaaggcac tcctcggtca tctgcgcggc
     8821 gagccacgcg agcgcggcgt ccacggcgga ggccgcttcg ccatcagcat acggcgcgag
     8881 cgcctgcacc accatggccg tgatgtccgt atccgaagcg ccggacacga gaccgaagcc
     8941 accgtccggc tcctgcgccg agaggatccc gttcaggata tcctcgcgcg tataccggct
     9001 gcctttcggc acttcataac cgccgcagtc gagcgcgatg agcgcgaaga tccagccatt
     9061 gagcccctgc atgccgagag aatccccagc atagtcgtaa acgcccccgg caatcagatc
     9121 gaccgagccg tccgtcccgc acgcggtggg atcgccgccg agcgcgcgca ccgtgagtgc
     9181 ggcgcgctgc cagtcggtag cgacaggggc ccgtccgttc gccgggagcg ggaagttggc
     9241 ggaaatatat tcgttttccg cggcgagata cgccgcgccg tcctccgcgc cgagaagcgc
     9301 gaaggcaatg gccgtccagt cgcacacgga cgtgcccgcg gggaaatcct cgccctgcgc
     9361 gagcagctcg ccgctcacgc ccgcgaggaa cttctgcccc gagacggcgg agagtgccag
     9421 atctctggca ctctccgc
//
LOCUS       DAWAAE010000078         9377 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3308_length_9377_cov_3.228384, whole genome shotgun sequence.
ACCESSION   DAWAAE010000078 DAWAAE010000000
VERSION     DAWAAE010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3308_length_9377_cov_3.228384"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5007..5016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1912 a         2951 c         2697 g         1807 t
ORIGIN      
        1 cgatcgaaac gccgagaccg atgagcgccg gggcgtgatc cttcgtctcc agccactgct
       61 cggtgaacac cgtgagaaag agcgccgtga gcgaaaagtc gatgccggtc gtgttaaagt
      121 ccagatacgc gcccgcaagc gagccgagcg ccgtgccgag tacccagtaa agctggtcaa
      181 gcagcgagat acggaagcac tccgcgccgg tgagatcctc gcgcgcggcg acgagcgaat
      241 acgtctcgtc cgtcagcgag aaaatgagat acggcgcgta ttttcccgcg gcgcggtagc
      301 gttcgagcat ggaaatgccg taaaaaagat ggcgcgcgtt cacgagcagc gtggtaacgg
      361 ccgtcgtcag gagcgacgcg cccccggtca gaaggtcgat ggccacatac tgcatcgacc
      421 cggcgtaaat aaacgcgctc atggcaagcg cccagccgag accgtagcca cgcgtgcgca
      481 gcagtatgcc gaagcccgcg ccgagcacaa gatacccggc catgaccggg attgtcgtgc
      541 ggacagactt tccccatttc tgcaaggatg tcatcgcaga tcactttcct actcgtttag
      601 tatgctgtta gaatacgacg atgcagcctc tctgtgcaac ggcaaaaaga gagaaatcgc
      661 gccgccgcgg cacgcttccc tgcggcaaat tgccggagaa taaagaaagc gcacccgaag
      721 gtgcgccaaa agccccgcag aacgtcaaaa cgcctcgcag agtttgcgcc cgcaggcgca
      781 aatggggttt aaatcatttt tgccggcgac ctgtgcgtcg gcaaaaatcc ttctcggctc
      841 gcaaacgtgc gagcgcagcg agtgcgctgc ggcgagccgc gatcctctca aatgggagag
      901 acctgccccg cagggctctc ccatttgaat ataaaaaagg cgcacccgaa ggtgcgcccc
      961 ctccgcaatg ccgtaggggt cagattagaa cctgcacgac tcggcaggtg ggtcgccgcc
     1021 cgatcgcttc cgcgcttcca acgtccggca aaaagccggg aggcctgcaa ttcacggccg
     1081 gagataggcg tcccttatat ctaatgtggg tttaaaatga cccggtgaag tgtggctacg
     1141 agcaccgcag aaagaaaatc ttattccttc tcttcttccg cttccatgat ctgctggtag
     1201 tattcgtaca cacgatccag ctcgtcatcg tcgtccacgg agtcgaaaag ctcctcgccg
     1261 ttctcctcgc ggctcttcag gaagatgaga ccgtaatcgg gatcgttcac gtccatgttt
     1321 tcaatgtctg ccgggacgaa cacgcagtac gtctggcctt caaattcaat agtatcgagc
     1381 agctcgagat caaactcgtt gccgtcctcg tcggagatgg taatgatatc gccgccgaaa
     1441 tcttcgttca tgtcagcacc tcttttgtat ggatcccgtt cgtttccttc gggtattttg
     1501 attataccat gaatgcggta aaaatcaagt gtatccgctc gaatggttca cgacccgaaa
     1561 tcttcgagcc gcgcgtacag ctcctcgcgg ctgtatacgg cgatgccgcc ggagagcgca
     1621 gcgcggtcgg cgctcggcag cgtgtgcacc gcgatggccg tgatctgccg cggcagcgtc
     1681 cgctcgtccg gcgcgaagat cgccacgcgc ccgtggtatt cccccagcga gcagtattcc
     1741 gccttctttc ctcccgaaag cgcgaacatc gccgcgcccg ccgcggcgca cagcgcgagc
     1801 gacaccgcgc cgatatgacc tgctttcatg gctcttttcc tccgtttccc tgccgccttc
     1861 tccgcgcagt atgcccgaaa atgaacgaat tacccgtttt ttccgtccta tggatatacc
     1921 tgctttaaaa aatttccatt tcggatacaa tatttctcag aagtatggta taatgactta
     1981 ttattatacg cacatgctcg acgattccgg aggcagctat ggatcagaaa cgcccctaca
     2041 acgatatacc gaacggcgat accgcgcccg tgccgcccgt atccggcgat acggtgccgc
     2101 ttcccgcacc gggggaggga acgcccgcgc cggagacaga caaagccgcg ccggaaaacg
     2161 gcgcgccgtc tcccgagacc gccaagaccg ccccggagcc ggagaagaag ccggacgcga
     2221 aaaaagcaaa aaagaaagcg ccgcgcgaaa agcgttcccg cggcatgtcc cggggcgcgc
     2281 gcaccgcgct cgccgtcggc gacgccgcca tatccaccgt gagagatatg gtgcggttca
     2341 ttgtccgcat cgtgctctcg gtgctgctcg tgctgctgct gggcgggctt ctcttcgcgt
     2401 gcattttcgc ctactatgtg aagaataccc ttacgaccga tctgaacatc acgcttgagg
     2461 actacgccat ctcgctctcg agcacggtct gggcctacga taacaacggc cagaccgtag
     2521 agcttgcggt gctggcaacg gacgagaacc gcatctgggt cgattacgac gagctgccgc
     2581 cgtatctcga aaaggcggcg gtcgccatcg aggacaagcg cttctatgac cacaagggcg
     2641 tggactggta ccgcacggcg ggcgcgttcg tcaacatgtt cctcggcatg aaaaacgact
     2701 tcggcggctc gacgatcacg cagcagctca tcaaaaacgt cactacggaa gacgacgtca
     2761 ccgtgcagcg aaagctcgtg gagatcttcc gcgctctcga tctcgaaaag gaatacacca
     2821 aggaagagat catggagtgg tatctcaacg ttgtctactt cggcgagggc gcgagcggcg
     2881 tgcagatggc cgcgaacacc tatttcggca aggatgtgcg cgatctcacg cttgcgcagt
     2941 gcgccagcat catcggcatc acgaacaacc cctcgcgcta cagccccttc gccagcgtgg
     3001 agaacaacaa aaagcgcacc aagaccattc tctacgagat gtatgaccag gattacatct
     3061 cccatgagga atataccgcc gccgtcgcgg aggtcgataa cgacgagctg cacttcatcc
     3121 gcggcgaaaa cgaggtctac cagcaggaga tctattccta ttatgtggaa atggtcatca
     3181 aggacgtgct gagcgatctg cagtcacgtc tcggtctttc cgaggccgcg gcgcgccagc
     3241 tcctgtacca cggcggctat cagatctacg cctgcgtcga tcccttcgtg cagcaggtcg
     3301 tggacgatat ctataccgac ccggacagca tgccgcagcc ctattactac agcagccagc
     3361 cgctgcagtc cgcctgcatg gtcatcgacc cgttcaccgg cgagatcaag gccatcggcg
     3421 gcgcgattgg cgaaaaggac cgcaacttcg gctttaacta cgccatcgac gcgcaccgcc
     3481 cctccggctc ctcggcaaag ccgctcgcgt cctacggccc ggcgatggat ctcggcctta
     3541 tcaccgagtc tacgctcgtg gacgacagcc ccgatatcca gctgcgcggc acctactggt
     3601 acccgcgcaa ctctggaagc tcctacgacg gcatcatcac catctgggac gcgctggtgc
     3661 gctcgaagaa caccgtcgcc gcgcagatca tcgataagct cacgccgagc gtttcctttg
     3721 aatacctcac cgagcgcatg ggcttccagc tcgtcgaggc ggaccgggac tatgcccccc
     3781 tcgctctcgg ccagttcacc tacggcgtaa cggtgcgcga aatggcgcag gcatacacct
     3841 ccttcccgaa cgacggcgag atgacgtacg cgagatcgta ttcgctcatc accgattcaa
     3901 acggcaacac cgttctcgaa aacggcatgc gcacgagcca tgtgttcagc gtggacaccg
     3961 cccggtgcat gaccgaaatg ctcgaggccg ccgcgtggta cggcacgggc tacgaggtct
     4021 atctcggcta cggcaaaatg cccgtcgccg gtaagaccgg cacgacctcc gatgactacg
     4081 accgctggtt ctgcggtttc tcgccgtact atgtgaccgc cgtctggacc ggctacgaca
     4141 gtcccgcgcc catgtactat tccggcaacc ccgccgcgca gatctggcgg cgcatcatgt
     4201 cgcagatcaa cgagccgctg cccgtgatcc agttcaacga cccgatgaac cgcggctacg
     4261 acaccggcat tttcggcgat cttgaaacga cgccggagcc gacgcccgtg ccgaccgcga
     4321 cgcccgcgcc cacggaggcg ccgaccccgg agcccaccgt ggaaccgact cctgcgccga
     4381 cggacgagcc gcagcgcatc cctctcgcgc cgtcagcgcc cgaggaatga acggtctcgt
     4441 cccgcgcctt cccaaaacct caccgccccg aagaaaaaat cttcggggcg gctttctttt
     4501 tcaggaagat ttcagggaat cgccgtatag cagcaccatc aaagcaaagg agacgccacc
     4561 atgaacggat atacttttaa cggcgggagc tcaaataccg catcacggac gcgcaccgcg
     4621 cagcgctcga ggccgcgttc ggtgcgcgga tggttccgga tgagtatggc gagagcacca
     4681 tctgtaatat atattacgat acggccaatt accggctcat ccgcgcctcg ctcgaaaagc
     4741 cggcgtacaa ggaaaagctg cggctgcgca gctacggcgt gaccgagccg ggcggcgagg
     4801 tgtttctcga gctgaaaaag aaatacaagg gcattgtgta taagcgccgc atcacgctgc
     4861 cggaggacgc cgccggggaa tttatcgccg gacgcgcccg ctctgtgaac aaccccctca
     4921 aagcagccaa cagccgcagc ggaaaaccgc tgcggctgca gtctgtcgaa aaagcctcgc
     4981 agagtttgcg cccgcaggcg caaatannnn nnnnnntcgt ttgagagcgt gtcaaaaata
     5041 cttttttgag atgctcaagc ccaccggcgc agccggtggg cttgactctg ccgccgaaat
     5101 tgctttggcg gcggggcttt tatccatttt tattcgccga tgcggtagac gcgccccttt
     5161 tccttcgcga ccctgtgcgc gttgaacgcc gccggtaccg cgccgatggc cgagaaaaga
     5221 tagagcgtga caagattgct ccgataggcg tcggcgtcca ccagctcgtg catccacgcg
     5281 aacgcctgac cgaaggagag atccagttcc cgcgatgccg cgatcgcaat gtccatacgg
     5341 ttgccgagat agaccgcagc gagcatcacg acgacgctca cggcgatgcc gagagcggag
     5401 agcttgcccg cgccgcggcg gtagccgccg atggtgcagt agccgagcac catgccggag
     5461 atcgccgcaa catagccgag ctgaccgatg agcacgatcg ccgccgcgcc gatcagcccg
     5521 ccgatgagcg cgccgaggat gccgcggccg atgttttcgg gcgtttcggc ctgaaggcgc
     5581 tcgttctgat ccgccttgcg gctgagttcc gtaaagagcg ccggcgcgac aagctgcggc
     5641 acgccgctcg tcatgcagcc gaccgtttcg ccgacggcgc gggagatctc gcagcagttt
     5701 tcgtagccgt tttcatgcag gaacgagaga agctccggca ggagcgtgtg gatcttcccg
     5761 gcatagcttt tcgccgcagc gccggggcgg agggaaaaga tgacgcgcgt ccccgccgtt
     5821 ttgcagccgg agagctccgg ccgcgcctta acaaagtcgc ggaagctctc tttatccggc
     5881 tcttccccgc cgcgtagaac ggagacgaca agctcgttgc gccccatcag gatcagaaac
     5941 gtatattccc cgcgcgtgcc gcagtaggtc gcgccgtcgt gcgccatgga aaggccttcc
     6001 tgctgaagca gcgtatccga aagcttcatg cttttttgtc ctcctccggc gcgttgagcg
     6061 cggcgatctc ttcgggggtg aagttgtaca cggcgtcgca gaactggcag cgcacctcga
     6121 tatccttctt ctcctcgcgc agcgacgcga gatcgtccgc ggaaaggctc gcgagcgcgc
     6181 cgagcacccg ctcgcggctg caatagcact tgtacgccat atcgctctcc gccgttttgt
     6241 gccattcaag gcccttgaac acctgcgcga tgaccgtctc cgcgccgtcc tcgtcgagga
     6301 tggtcgtaag ctggtccatg aggaagatgt tgtcctccag cgccttgacc gtctcctcgg
     6361 cggcgttcgg catgagctgc atgataaagc cgcccgcggc cttgacgctg ccgtccggat
     6421 tcacgagcac gccgagcgcg cacgccgagc cgagctggtc gctctcggtg agaaacgccg
     6481 cgagatcctc ggcgatctcg ccgctcacga gcgcggagga accggagtac ggctcgcgca
     6541 ggccgatgtc gcggctgacc gtgagaagac cctccctgcc gaccgcgccg gagacgtcga
     6601 gcttgccgtc ccgctcgcgg atgggaagat cgacctcggg gtgggagacg tagccgcgca
     6661 cattgccggc attgtccgat accgcgagga tcgtgccgat cggcccgccg ccctggatgc
     6721 gcagcgtgag cgcggcgttg tcctccttga gcatttctcc gaggagcgac gcgccgcaga
     6781 gcgtgcggcc gagcgccgcc gtgccgacgg ggtgcagatg gtggatctct ctcgcacgct
     6841 cgaccatgtc gggcgcggtg atgacggata tttttgccgt gccgtcgttc gtgacggcgc
     6901 gaagaatttt gcccatggtt ttttctcctt ttagaaattg acttgcaaat ggctctgtgg
     6961 gaacggctcc cttgtgtaaa gggagctgtc agccgtaagg ctgactgagg gattgtcgtt
     7021 tgacgaagca cgcgacagtc tgcaatccct ccgtcaaaac ctgcggtttt gccacctccc
     7081 tttgcacaag ggaggcattg gtttcttttt actccttcac gcactcggcg gtgaagaaga
     7141 gccgtccctg gttgtgctgc gggccgtccg taaaaaggcg gatgctgccg aagcccgcat
     7201 cgacgaggtg cttttccagc gtttccggcg tatagatata ctccgtatgc tcttcctgct
     7261 cacggcggta ggtctgcccc ttgatgcggc ggaaaatgtc catgccgtag cgcagctcgt
     7321 tttccacgac ctccgagcgc cagagacaga gcgtatcgtc ctcctcgtcc acgaagcact
     7381 gcccgtcgag cgagcgcata tgccccggcg agaggaagtc aaagcagagc gtaccgcccg
     7441 gcgagagaaa cagatgcagc cgccggaaca cctccggaag cgtttcgggc cgcatgtagt
     7501 tcacgccgtc gagcgagcat accgcggcgt ccacggtgtc ataaagatcg agtttctccg
     7561 cccgctggca gagaagcatc ggcgcaacga cgccctccgg cagctcgcag agcttctgct
     7621 ggaacaccga gagcatttcc tccgaggcgt ccacgcagat catctcgtac ccgtgttcgg
     7681 ccagaagcag cgagagcgta cccgtgccgc agcagagatc caaaaccgta tgaacggccc
     7741 tgccgctgtg ggcaaaggcc gtttcatacc atgtctccag cgcttcgtac ggcacgtctc
     7801 cggtcagcgc gtcatagcgc gcggcgagag gtccatagca ctggctcatt tttcttccgc
     7861 ttccttctct tcttccgctt gctgctcctt cagacggcgg aaggctacgc tgtagccgtc
     7921 ctcgccgtac tgcagtgagc gattcacgcg gctgatcgtt gcgcttgaag cgccggtctt
     7981 ttccagtatg tcgttataga tcatgccctc gctgagaagc accgccacct gatagcgctg
     8041 ctccatcgcc tgcagctcgg ataccgtgca gaggtcgtca aagaacttca tcagctcttc
     8101 cacggtattg agcgtaagga tcgccttata catttcctcg ttgcgcggtt ttttcaagtg
     8161 tttgttcata cagcgtttta cctgtgccct ttttcatttt agcttattag ctaataactg
     8221 tattgtaacg cgcggcggca aaaaaggcaa gatggagctt tacacgattt aaagagtgat
     8281 ttttggtgag aatgttattg ccgagcggcg gaaaatacgg tatgatagat acaaattaaa
     8341 ataagggagg ttttttccgt gatcccacct tccggaacga tcgcattctg ggtcgtggcg
     8401 ctcgtcgtgt ttctcatcgt tgaagccgtg accgcgggac ttgtatccat ctggttcgtt
     8461 ttcggctcgc tcgtggcgct catctgcgcg gcgctcggcg cggcggtgtg gctccagatc
     8521 ttctggttcg tcattgtctc cgtcgccacg ctcgttctca cgcggccgct cgtgaagcgg
     8581 tatgtggaca gccggagcgt cgctaccaac gccgaccgca gcatcggccg cgccgccgtt
     8641 gtcaccgagc gcatcgacaa tctcgccgcc accggcgccg taaagcttga cggcgttgtg
     8701 tggaccgcgc gcagcaccga cgatgccgtc gccattgaga ccggcgagcg cgtcaccgtc
     8761 cgcgccattg agggcgtaaa actcatcgta gagcgcatcg tgcgctgaat ctgcactgta
     8821 ttttaataag gaggaaatcc catgatcgta ttagccatca ttctcgtgct ggtgctcgta
     8881 ctcatcatca cgaacctgtg cgtcgtacag caggcgcagg cctacgtcat cgagcgtctc
     8941 ggcgcgtaca gcaccacgtg gggcaagggt ctccatgtga agctgccgtt catcgagcgc
     9001 gtggcgcgcc gcgtctcgct caaggagcag gtcgcagact tcccgcccca gcccgtgatc
     9061 acgaaggaca acgtcaccat gcagatcgac accgttgtct acttccagat caccgacccg
     9121 aagctctata cctacggcat cgagcagccg atggtcgcca tcgaaacgct ctccgccacg
     9181 acgctccgca acatcatcgg cgatcttgag cttgaccagt gcctcacgag ccgcgacatc
     9241 atcaacagca agatgcgcgt cattcttgac gaggctaccg acccctgggg catcaaggtc
     9301 aaccgcgtgg agctcaagaa cattctcccg ccgcgcgaga tccagagcgc gatggaaaag
     9361 cagatgaagg ccgagcg
//
LOCUS       DAWAAE010000079         9338 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3325_length_9338_cov_3.146935, whole genome shotgun sequence.
ACCESSION   DAWAAE010000079 DAWAAE010000000
VERSION     DAWAAE010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3325_length_9338_cov_3.146935"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8880..8889
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1928 a         2642 c         2779 g         1979 t
ORIGIN      
        1 ttgaccatcg tcgcgggcgg atcgatcagc tcgccgatgg caagagcgat cgggaacgcc
       61 acgttgaggt gatccgggaa gaacatcgag cagagcacca gctcgcccgt gccgccgccg
      121 ccgggaatgc cggacatggc aaccgacgag aacaccgcca caacgatcgc caggatcgcc
      181 tcacccgtac caaagtcctt gccgaacata ccgaagagga acgcgacctt gatgatggcg
      241 cccatggccg agccgtccat gtgcatcgta gcaccgaggg gcatgacgat atcggaaact
      301 tccttggaaa tgccggtatc ctccgagact tccatgttca ccgggatcgt tgcgaccgag
      361 gagcatgtgc cgaacgcggt cgccgccggg cggaagaggt gccggaacat gaccttagcc
      421 gcgcccttgc cgccgccgaa gcgggcatac agcggggacg aaatgagcag gtagatcacg
      481 gaaacgacat agtaaatacc gatggcgcgg gcgtagttag agacgaagtc cgagccgtgc
      541 gtcgcgacga gggaggcgaa gaagccgaag aagccgacgg gcgcatagta ggagatgagc
      601 ttgatgacct tcagaagaat gtccgtgagg tcattgagca gcagcgccac gcgggactcg
      661 cggccgccgc acatctgaat gccgaagccg aagatgatcg ccgcaacgat cagcggaagg
      721 atggcgcggc ggctccagag ctcggtgaag tcgggcttcg tgaagaagtt gacgatgagc
      781 gtgccgatat ctacggtacc gacgccggac tcttcatagc tgggcgcggc ggtaaacacg
      841 gggataaagt gaacaatgat gtacatcaaa atcgcagcaa tggcggcggt ggaaaggaac
      901 gttccgaccg tgatgcccat gatgcgcccg gcgcgttttg ccgagctcat gtttgcgatc
      961 gccgaggcga tggagcagaa caccatcggc acaacgacac agaacatcat attaataaac
     1021 agcgtcccga acggttcgag agccgtagcc cccggccatg ccgcaccgac aatacagccg
     1081 gcgacgatgc cgagaagcat cagtgtaatg aaaccgtatt tcttcataaa cttcttcatt
     1141 gcacataacc tccactgagt attttcgtgc tatattttac tgcgcacgcc ttgcaaagta
     1201 aacgcaatct atgctatact ctttgcaaaa gatgctttta tggaggccgt catggacgaa
     1261 aactatgaaa agcgcggata cctgctggag gatttccgac tctttcacct gaaagacgac
     1321 cgcggcacga aggttgatta ccactatcac gaattctgca agatcgtgct tcttctctca
     1381 ggcagcggca gctataccgt agaggggcgg cgctatcttc tgctgcccgg cgacatcgtg
     1441 ctcgtcggca gccggtgcgt acataagccc gaatttgaag agggaatttc ctatgagcgc
     1501 atgatccttt atatctctcc ttccctgctc gcggaaaatt ccgtgccgga ctattcgctc
     1561 gaagacgtgt tctccggtaa gccgggccat gtgcttcgcc cggcggagga cgtccggcgg
     1621 cggcttcgct cgctggcaga gcagctcgag agcgaaatgg ccgcgagcgg cggcgagaat
     1681 gcgatccttt cgcggtgtac gctgctgcgc cttctcgtgg aaatcgggcg tgcaatgcaa
     1741 aaaaccggct ccgagctccc gcagccggtc gtaccgaggg acggcaagat tctggacatt
     1801 ctgcgctatc tggaagccaa tcttgccgaa gacatctcca ttgacgatct ggccgcgcag
     1861 ttttttatca gcaaatacca tatgatgcgc cgcttccgcg aggagaccgg cacatccatc
     1921 cacaactatc tctccgacaa acggcttctg gcggcgcgcg atcttatcca gtccggactt
     1981 tccgccacgg acgcatgctt ccgctgcggc tttcgcagct attccgcctt ttcccgcgcc
     2041 tacggaaagc tcttcggcgc cacgccgacc ggacgcatcg tgcagacgct ccacgcggac
     2101 gactttgacg aatgacaggc aaagaggtgc atccaatgaa atttacaaaa atgcaggggg
     2161 ccgggaacga tttcatcgtt ctggacaacc ggtccggcga aatacgcgag aacgaatact
     2221 ccgctctcgc cgtccgtctc tgccgccgcc gtctctcgat cggcgcggac gggctgatga
     2281 tcgtcctccc cgcgcagggc atcggcgatt acgccatgcg gttttacaat tccgacggct
     2341 cactcggcga aatgtgcggc aacggcgcgc ggtgtatcgc gcgttacggc tatgaccgtg
     2401 gtctcgccgg agacatccag cgcattgaaa cgaccgccgg gatcgtcgtc ggacagcgca
     2461 tcgaagagga actctactgt gtccggctga acgaccccac cgctgtggag accgagatcg
     2521 gcataccgtg ggacggacag atcctctcgg tatcgtatat ggaactcggc gatcccggta
     2581 tcccgcatgc cgttttggag ctgccggaat gggacatgat gccggaggat gagctgcgct
     2641 cgctcggcgc ttacatccgc agctacgccg cctttccgaa gggcgcaaat gtaacgttcc
     2701 gcaaacgcct tggtcgggat catgtaaagg cggtcaccta tgagcgcggc gtggaggact
     2761 tcacactcgc ctgcggtacc ggcgcgggag ccaccgctgc cgccatgcgg ctgcgcggcg
     2821 agatcagaga tgaaacacta cggctggatt tccccggcgg tgagctgcag gtatcgctct
     2881 ccgtggatga gaacgctgtc tgcgatattt atcttaccgg cccggcggtc gttgtcgccg
     2941 agggcgagta catcgggagg taacgccatg agcaaggaac gcgatatgcg ccgtacagac
     3001 tggaaacgca tcacgaagcg gcggtacgtt tcccgcgggg aagctgacat cttcggcagc
     3061 gccgggcgaa tttctcttac tttgattgac gaggtgaccg ggccgctcac ggtgcactat
     3121 aaaagccggg ctgttctcat cgctgacgcc ggttattcgt ggtttcaggc cgccgttccg
     3181 ggcgcgcgct ggtggctgac ggcgatgttc gacgagaggg accggatcat acagatttac
     3241 tttgacatca ccggcggcag ccggtttgac gatccggaaa atccgacgtt tgaagatatg
     3301 tatctcgaca ttgtcgtatc cgccgaaggc tcgattgagg tcgttgaccg ggatgaactg
     3361 gacgaggcgc tgcaaagcgg cgcgatcacc gtcttacagc acagggaagc aatcgaggcc
     3421 tgcgaaaagc tggagaagtt tctccgtgaa aattcgaccg ccgtcctgga ctggtgcagc
     3481 gccatgcagc ggaaactgaa aagagaaatg ccggaatgat tccaaaaaga gggagaagct
     3541 gcgtgagctt ctccctcttt tgggttgtta aaaagctgac cctccgggag tttcccggag
     3601 ggtccgtttt gctcagtcga tcttataggc gcgcttggtg acgtcctcgc gttccatttt
     3661 gtcgtaatgc ctgcagagga acggctcaac cacgtagtac agcagcttgc cgtcgaggtc
     3721 gaaatagaac accgcaaaca gcagcgtata aagccccgcc agaatgccgg cgatcttgcc
     3781 gaggatctta aacagtttac gcattctttc ttcctccctg ttaccagctc atggaatcgt
     3841 caaactcaag cagcgacgga tcgagcggct cttcgtgcat gacggtggtc atgtaatcgt
     3901 tgatgatgcg gcggatctcc gcacgggaaa cggtgcggct gtccggcaga gcgtgaggaa
     3961 tccagatggc gtggatgttg cgcttgcgga actcatcctc gaacacgccg tgcataccct
     4021 gcgcgccctt gcactggagc tggtcgtaca tggcgaccat gtcgcagttg aactgctcca
     4081 tatacttcca gagctcaaag aagtggtgca tgccgccgac tgccatacgt cgcatggggg
     4141 cccacatgtg gtactgcgcc atatcctcga gcgtgttctc gtagctgtcg gtgcggatgg
     4201 tcatgtcgaa catgagagag tccatgttga tgatgacgtt gagaccccag cagttgtacg
     4261 cccaggtaca ccagttggag taatacagcg gcgcgcacga ccacgcgatc atgcggtggc
     4321 gggtttcggg gaaggtcacg gtcttgttcc gcacgcagcg ctccataatc ttgcggacgc
     4381 gggcatcgac ctcatgccag atgaagtttt ccccgtactg cgactgatag atgcggtaga
     4441 gcgcctgcgc aacgccgttg atgggatagt agttggtgtt cgcggcaaca tcccatttct
     4501 cccactcgaa ccgctgcagc tcgttctgag cttccagatt tttgacgagc tgatcccagt
     4561 tgaacggcac accggtcatt tcctcaacga atttccacac gccctcgatc tccttggcgc
     4621 agtgcttctt gacgagcgga tcgtcaaact gcatcggctg cggcatggcg tagagcggct
     4681 tattgaagaa ccagtcctgc agagcggtgc ttgcaacgga agcgtcgcac gcttcgttgg
     4741 acgtgatgaa catcggggag taatcgggca cgtcgtcctg cacaaagata ccggcttccg
     4801 cctggcacat cgggcacggg tcgcccggca gaccgatgga ctgcacggcg tcgatgtagt
     4861 ggagaacggt gtggctgttg acgtggcagg tgacaaaata cgggatcatc tcgagcggaa
     4921 cgtgtttgaa tttatccgcc cacggataga acacgccgcc ccataccgtc tccttgtggg
     4981 cgatggtatg gtcgtaataa tcgttctgct tgccgttgtg cagcttcgtg tcggcggcga
     5041 acatacgctg gaactgctga atggtcatgc tgaccatggc acggtagtgc cacgcggagg
     5101 cacgcagcgc ctcgccgcgg agtccctgca ggccgcggtc gaaccagtgc agaaccggaa
     5161 gataggtctg gccgacccag cggtagcgcc acacgccctt gaagaaggcc agaggctctt
     5221 cgctcttgag aatgtccgcc accatctgcg cgtagttgta cagccagatg ttattgaagt
     5281 agtacatcgt gtcctttacg ccgcgccact cacggtgctt gtaaaggccg gtcttgtcct
     5341 ccaggatatc gccgagacga cgctcgccgt gcggtttgcc gtaccagaaa tccttcaggg
     5401 cttttttcat tttcgcctta tccattcttt gcgccctcct tctcctggat cttcttgatc
     5461 tccagacttt ccacaaacgc ctggaagcgc gtgcggagct ggccggaggc ggagttgatg
     5521 tactctttct cgatcgagca cacgggcaga ccgaagtcgc gcggcaggat gatcgtatcc
     5581 gccacacgct catagctcca gtactcgcag aacttgttgg attcgacgat cacgccgtcc
     5641 gccttgtatt ctttggcgac atcggcgatg tactttttgc gctgacgcat ggcgctctgc
     5701 tccatggagc gcgggcaaac acttgtttcg agatagtggc gggcgacagc gcgaagcgcc
     5761 gtctcccctt ttcggatctc gatcttctcg cgtccgggca gagagccata gcagtagcgg
     5821 tcggccacga cgagcgcgcc gcagctctcg atgagcttgg tgaaatcggg atcgtcattt
     5881 tccgaaccgg cgagaactac cttaacgcgg tagttgggct ttgcatcggc ttcgcgggtc
     5941 ttgagatcct ggagcgtttc acgcagatac gggaggatca ggtactttgg gcagaccatc
     6001 gagacgagaa tgatgatgtg gaactccgtg ccggtgatcg tgggcttatc gagcttgcgg
     6061 aattcgccga tctgcgtgat gatgtcgcag atctcgttgt gcgcctcgat cgtggcgcgc
     6121 agagcttcgt cggacgtgtc gatgccgaaa tgttcgtgca gctcgtcgag aacgtgcttc
     6181 tgcagcgtgg cggcaaagtg ttcgatgctg ttctcgttct ttttgaacgg aacatccgtg
     6241 aaggagacga agaatttttc gttctgaatg aggtcaggca gctcggcgag atgctcctga
     6301 aaccggcaag tgccggcgca ggtctccgtg ccgagctgcg cgtcaatgaa gttgtagccg
     6361 ccctcgagcg cacgctcgag aagactgcgg gcatagtggc agacgcggct ggacatataa
     6421 taggtggcca tatccgggga ggtgcagcgc ggggcgcgga ggcgaacgct gaaacagccg
     6481 ggcagatcca gaaggacctc cggcatgaaa tagcaggtgt agccgagagc atactttccc
     6541 tcttccttcg cctgccggac aagagcgtta taggactcct gcagcagatc gtcgaagaag
     6601 aacacatttt tcaaatcgcg catggggctt cctccgttga tgctttctta ttccgacggg
     6661 gacttttttc ccgcctgctg gaagtgaagc tcacgcatac cctcaacctc gtcgcagatc
     6721 tcgcgcagtc cgcacgagtc gcagtcggtg ggtataccgt ccagaatggt gctgagagaa
     6781 cgggtaatgt cggtgacctt gcgcgcctgt gcacgaaggc cgggataatc cgcctccggc
     6841 gccgtgacga agatcacgcg cacaccgcga acattttcct tctcgcggaa tttgcggata
     6901 tagtcaaagc cgacgttccg gaaggagatg ccgccgcgga tggcgtcgcg gctgacgcgc
     6961 acctgctcgc ggttatcctc cgagagcgcg cggatcatgt atcccttcgg gaagacatgg
     7021 tatttgacaa attcgatatc ctgcagcgca cggtacgctt cgtcctcttc tcccacgtcc
     7081 tgaatggaga acagaacgat gcgggcaaag gcgcagtcgc cgcggatctc cgaaagatcg
     7141 gggccgtaca gccagacctc gtccggcccg acaagacgct cgtcggtcgt aaagcagcta
     7201 taggacacgg cgcttttggc gctgccgccg agctcatacg ccgcttcgcg gagcatgacg
     7261 agctcgtttc gggacgtttc cgcccagcag gacgccgggt catacgccca ttttttcgcg
     7321 gaggagccgc tgagcagctc ctccgtttca tgaatgcatc cgttaaaaag ctccatcaga
     7381 acttgatgtc cgctttcttg cggtcaaaga tccggttgac ctgtacatac gcccaggaga
     7441 gcagcttctg gtcaagattc cagaaataga ccacaaacaa tacgccgatg actgccgtca
     7501 gaataccggc gatcttacca aacactttcc agaatttttt catagggaat tatactcctt
     7561 tcgaaagtcc cttctggcgg gcgtattccg ccgcgccgag agcgcccatg agctgcgggt
     7621 cggtcttgag gtcgatgacc ttgagcttga ggacgtgctc aatggcctgc ttcaggccgg
     7681 cgttcttcgc gcagccgccc gtcagggtga tgctgtcggt cgcgcccgct ttcttcgcca
     7741 taacgaagca gcgcttggca acggccatct ggatgccggc ggcgatctcg tccatgggct
     7801 tgccttcacc gacgagcgtg atgacttcgg attcggcaaa gacggtgcac tgggcggtga
     7861 tggggatgac attcttcgcg ccgagagaca ggttggagaa ttccggcagt gtcatctcga
     7921 aggagcgcgc catcgcttcg tagaaacggc cggtaccggc agcgcacttg tcgttcatgg
     7981 caaagttctt gacggtgccg tcggtatcga tgctgatgcc cttgacgtcc tggccgccga
     8041 tgtcgatgat ggccttgacg gtcgggtcgg tgacatggac gcccatggcg tggcagctga
     8101 tctcggagac gttctcgtcg gcaaagggga ccttgttgcg gccgtagccg gtgccgatga
     8161 gatactcaag gtccttggag gaatgcaggt cggggacctg atcgatgact tcctgcatgg
     8221 cgatctgggc ggactgctcg gagttgatct tgctgcggat ggtggtgtgc gcgacaatgt
     8281 ttccctgctc gtcaaggatg actgctttgg tgtaagtgga accgacatcg catccggcga
     8341 aatacttctt ggccatggtt aaataccctc caataatgat tctatttctt tttttacagg
     8401 cagatctcgt cttcaaaatc aaccagcgtg ggatccaaag gctcggcatg cataacggtg
     8461 cgcatatact cgctcacctt gtcgcgcatc tggcggcggg agacggtacg cggatccatc
     8521 agatcgtgct cgatccagat catcttgcgg ttctgctcgc gcgcctgatc gtccagaatg
     8581 ccctgcacgg tggccatgtt cttgcaggca acgttctgat acataatgat gagatcgacg
     8641 ttccagatct cacactgctt ccacagctcg tcaacaacgt gctgataacc gccgttcgtg
     8701 tgacggcgca taaccatgcg ctcgtacaga cggccgagat cgcgcagcgc ctcttccttg
     8761 tcctctgtct cgagctgttt ggtgaagtta aggctctcca tctcaacgag aacgttgacg
     8821 ccccagcagt ttgcaagcca gttggagaag ttggcatagt agtgcgccgg gcaggaccan
     8881 nnnnnnnnnc cacacgatcg cgcggtactt catcttgccg ggccacgcct cttccttgcg
     8941 ctcgtaggcc ttgagcatga tcttgttgac ctttttgaag gtcttcaaga tgcgcgggtc
     9001 gatgccgccg tccatctcgt agttccattt gcggaacagc tcgtagcacg ggccgatgat
     9061 ctgcggatac gccgtctggt tgaccgccca cttttcgagc tcgtagctcg tctctgtgtt
     9121 gaagcgcttc atggcggaga agtaagcatc ccagctccac tttgcgccgg tctgctcctc
     9181 aatgaacttg atgcaggcct tgatatcctc ggcggcgcac tgcacggtct cctcgtcctc
     9241 gtagcggacg gggaagcaca gatggaacac cgggagatcc gggaagcggc gggacatgta
     9301 ggaggacgcc atggtcgagc cgtcgcacgg catggagc
//
LOCUS       DAWAAE010000080         9095 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3444_length_9095_cov_3.115819, whole genome shotgun sequence.
ACCESSION   DAWAAE010000080 DAWAAE010000000
VERSION     DAWAAE010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3444_length_9095_cov_3.115819"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4144..4153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7245..7254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1534 a         2892 c         2853 g         1796 t
ORIGIN      
        1 ggagacgggc aaaccacgcc gccgcgcgct gcgccttctt cttttcctct tcgctcccgg
       61 cgaccgtccc ggtctcgggc gcatcgccgc ccgcgccgca gagcggcacg gtctcgacga
      121 tgggcgtcgg gaaatcctcc gcgccgtcat cgaaaacgcc gtcgtccggt aatatcccgc
      181 cgaccatcac gctcgcgctc gccacggtag cgaccgcagc ggcggcggcc tgccgcttga
      241 gcttttctcg cttgttccga tccatttcag accatctttt cgggacggta cgcggcgtcg
      301 aacgcctcgg ccgtcatgta cccgagccgg aggcagctct cgcgcagcgt gagatcttcg
      361 cggtgggcga gcttggcgac ctcggcggcg cgctcgtagc cgatgtgcgg cgtgagcgcc
      421 gtgacgagca tgagcgagcg ctccatattc tccgccattt tttcccggtt cgcgcggata
      481 ccggaaacgc accgctcgtc aaaggacgcg atggacgccg caagaaggcg cgcagactgc
      541 aaaaagttat aaatgcacac cggcaggaac acattcagct caaaattccc ctgcgatgcg
      601 gcaaggccca cggcggtatc gttgcccagc acctgcaccg ccgtcatcgt gacctgctcg
      661 cactgcgtgg ggttgacctt gcccggcatg atggacgagc cgggctcgtt ttcggggatg
      721 aatatctcgc ccagcccgca gcgcggcccg gaggcgagcc agcgcacgtc gttggcgatc
      781 ttcatcatgt cgcacgcgag cgccttgagc gcgccgtggg cgaacacaag ctcggagcgg
      841 gacgtgagcg cgtggaactt attctccgcc gtcacgaatt tatggccgct caaggcgctg
      901 atgcgctcgg cggaaaggct gtcaaagccc gccggggcgt tcagccccgt tccgacggcg
      961 gtgccgccga gagcgagcgc gcgcagcggc tccacggcgg cgcgcagcag ctcaatgtcc
     1021 gtttccacgc tcgcgcgcca gccggagatc tcctgcgaga agcgaagcgg tacggcgtcc
     1081 tgcagatgcg tccgcccgac cttcagcacg tcctcgttct ccgcttccag ccgccggagc
     1141 gtctgcgcga agctctcgca caccgggaga aggttttcct ccagagcgat aacggcggca
     1201 atgtgcatcg ccgtcgggaa cgtgtcgttt gaggactgcg acatattcac atcgtcgttc
     1261 gggtgcagca gctttttccc ggtgatggcg ttgccgcggt tcgctacgac ctcgttgacg
     1321 ttcatgttcg actgcgtgcc gctgccggtc tggaacacaa cgagcgggaa gttcccgtcg
     1381 agctgcccgg cgaggatctc gtcgcacgcg cgcgaaatgg cagcgcactt ttcttcggtc
     1441 atgcgctgcg gcacgagcgc gcggtttgcc tccgccgccg cctttttcaa tatggcaaag
     1501 gcgcggagga tctcctccgg catcgtttca tggcccgctc cggcggggaa gttccggcgg
     1561 ctgcgctcgg tctgcgcgcc ccagtattta tccgcgggaa cggcgatctc gcccatggaa
     1621 tcgtgttcga tacgcatttt ctcttccggc ataatggcgg ctcctttttc ttcggtaata
     1681 tacggacgtg cggcaacggg gacggatctc tctgacagcg ttgtgtcagc aagaaccgtc
     1741 cccatggaca acttttttac ttgggctggc ggccgttgca gaggtagaac accgtcagca
     1801 ggccggggcc gcagtgcgcg ccgatgacga cgccgagcgc cgtgatcgtg atctcgccga
     1861 cctggggata tgccttgcgg atctcgtcgc gcacccattc ggcatcggca acgcagtccg
     1921 cctgcaggat gtggatctcc gtgccggtcg gatcgcactc gctgaaatcg tgcaggacgc
     1981 cgtcgcgaat ggcgcgcagc gccgccttgc gcccgcgtac cttctgcgcc acgtcgagcg
     2041 taccggcgtt cgggacatag agcacgggct taatgtcaag gatcgagccg acgagcgcgg
     2101 cgctgttcga gacgcgcccg ccctctttga gataattgag atcgtccacg gtgaagcggt
     2161 gggcgatgcg gagcttgttc tcctcgcccc aggcgacgac ctcatcgaag cctgccccgg
     2221 cttccttgcg gcggaccatg ttcatcacga gcagacccag accgccggag atgcagagcg
     2281 tgtccatcac atagagcttc cgttcaggga actccgcgcg cagcttgtcc gcggccttgt
     2341 tcgcgttgac gaacgacacg gacatcttct gcgacatatc gagaaagatc acgtccttcc
     2401 cctcgtcgag aaacgtcttg aggaagtcgt aatacacgaa ctcgttgatc atggacgtgt
     2461 gcagcttgtc gccccggcgc ataccggcgt agaccgcggc gcgggtctcc tcacggcagt
     2521 cgtcctcgcg gggctcgtcg ttcacggtat aagaatacgc gatgaacggg atgttgtgct
     2581 cctccagcca ggtgcgcggc aggtcggagg tggaggatgt ggcaatgatg taatcagaca
     2641 tagtatattt cctctctgta gtatagtcgg tgtcggttta tccgccgaag atcgcggaga
     2701 gtgtcttggc cacgctccgg aaaaagccgc ggacgctgtc ccagaccgtt ccggcggtgg
     2761 acagggcgtt ttcgccgctc tcgcggatct tctccaccgt ttcgggcagg tcctgcacgc
     2821 gctcggcaaa gtttgatccg ccgatgctct ggatcttgcg gaagagatcg gcaagctgct
     2881 gcgtctgcgc gtcgttgagc gtcatgccgt gctccgcggc gatggagcgg atgcgctcgc
     2941 gcagctcatc gtccgagagc gccgcgagct cgtcaaagaa gtcggtcatg gcgccgagaa
     3001 gttcctcgag ctttgaggtg tcgagctcgc cggagagcgc gtcggcctcg cgcagcgccg
     3061 ctgcggcggt ctcctttgcc tccgggtcga gcggctggca ggtgagcgtt tcgtacgctt
     3121 tgaatacggc gggcaatatg gcgagcgcgc cggtctcctc cggcgcggcg gcgacgatct
     3181 ccgcgtccgt caccccggcg gcggtgagcg ccgccgcaat ggcggtctcc gcgccggtga
     3241 tgttggaaag cgagagcgaa agcccttctc cgccggagcg gatgcggacg aacacgccga
     3301 cggaagccgc cttctccggc acatcggaaa aatacgtttc cgcgtccggc cgggaaagcg
     3361 ccagctccat cgccgtgccg cgctcaacgc cgagcgcggt gaatataccg tcggtctcgc
     3421 cgagcgtaag ctcttcgccg agcacgacgc agctgtcccc cggcacggca aacgccgccg
     3481 tggtgagcag cagtaaagca agaatacagg aaatgagctt tttcaaaaaa cggttgacct
     3541 cccgaaaata aacagcccac gagagcgcaa tgcgtccgcg ggctgtttta tgcgtctcag
     3601 ccgaatacct tgacgctcgt aatgaccggc tggttcactt cggcgatgaa gccgtccgcg
     3661 ccggtgacct gcacgctcgc ggcgatctgg tccacaatgt ccatgccgct cacgacgtag
     3721 ccgaacacgg cgaaatcgcc gtcgagatac gtcgcgtcgt tctgcaagat aaagaactgg
     3781 caggtgccgc tgttcttatc cgtgccgcgc gccatgccga tggcgctgcg cgtcatggag
     3841 agcgggttgt tgaagccgtt ggaggtgaac tcgccctcca cggtcgtgct cgccttgccg
     3901 gtgccgttct tgttcgggca gccgccgtaa atggcgaagc cgtcgatgac gcggtaaatg
     3961 gtcaggttgt tataaaagcc ctttttggcg agattgagga actgggacgc cgttttgggc
     4021 gcgctgtccg cgtccacctc gagccggatg gtaccgtaat tgttcacggt gatctccacc
     4081 tcgtgtttgc cggtccaggt cttgtttccg ccgcagccgg cgagaatgct cgccgccatg
     4141 agcnnnnnnn nnncagcgcc aacagaaatg cgatctttct tttcatgggt cgaacctccg
     4201 tttccaaaga aattcgggtc ttatttgctg gatttacttt ttctccgcga gagagcgcgc
     4261 gtaggcggca tagcgatcct ttctcgcctt ggcctcttcc gcgctggcgc ctctcgccag
     4321 aacatacatt ttgatcttcg gctccgtgcc ggacggacgg acgacgagcg ccgagccgtc
     4381 gctcagctca aagaacagca cgttgctgcc gacgatgggc gtcttttcca caacgccgag
     4441 accggggatc accacgtcgc cggtctggta gtcgcgcaca cgggtgaccg gcgtgccgcc
     4501 gacctccgcc gggggcgtct ggcgcagcga cgccatgagc gcgcgcatct cgccgaggcc
     4561 gtccacgccg tacatgaaga ggttcacggt ctcttcgctg tagaagccgt actttttata
     4621 cagctcgtcg atcgcgtcga tgagcgtcat gccgcggtcg tagtaatacg ccgccatctc
     4681 cgcggccatc atggaagcgg tcacggcgtc cttgtcgcgg acgtagtcgc ccatcatata
     4741 gccgtagctt tcctcaaagg ccaggatgta ctcgtaattg cccttcgcct tatattcggc
     4801 gagcttttcc gccataaact tgaagccggt gaacgtcgct tcaaagtgga cgtcgttcat
     4861 ctcgcagatg cgctgcacca tcgtcgtgga aacgatcgtg ctcagcgccg cggggtgctc
     4921 cggcatcgtg ccggcggcgc ggcgggcgtt gatgatgtaa tcgagcagca ggcagcccat
     4981 ctggttgccg gtgatgggga tgtactcccc gttgcgccgc gccagaacgc cgatgcggtc
     5041 gctgtccggg tcggtgccga tgatgagatc gctgtccacc ttcttggcga ggtcgatggc
     5101 gagatagaac ccctccgggt tttcggggtt tgggctcttc acggtgggga agttgccgtc
     5161 aagcaccatc tgctcctcga cagggtacaa atgcttcacg ccaaggcggt gcagcgcttc
     5221 cggaacgagc ttccagccgc agccgtggaa cggcgtatat accacgcgga aatcgtcggc
     5281 gaccttcgcg acgacatcgc ggttgatggc ctgcgcctcg acatttttga ggaacgcctc
     5341 gtcggtctcc tcgcccatcc actcgatgag gcccttttcg acagcctcgt caaagtccat
     5401 gctgtgcgga gcgcggaaga tatcggagtt ttccatgcgg cgggcgatct cggcagcgtg
     5461 ctggggcggg agctgcgcgc cgtccgacca gtagacctta tagccgttgt actccttggg
     5521 gttgtggctg gccgtgacgt tgatgccggc ctccgcgccg taatgccgga tggcaaagct
     5581 cagctccggc gtgggccgca gagattcaaa aatgcgcaca tgcacgccgt tcgcggccat
     5641 gatcgcggcg gcttcgcggg caaacgccac gccgttgttg cggcagtcgt ggcagacgca
     5701 cacgccgcgc ttcatcgctt ccgcgccctc ggccacgatc agctcggcaa acgcctgcgt
     5761 cgcgtgacgg atgacgtgga tgttcatgcg gttcgtgccg agacccataa tgccgcgcaa
     5821 tccggcggtg ccgaaaacga gcggcgcata gaagcggtcc tcgatctcct tgggatcgtc
     5881 gcggatggcg tcgagctctt tccattcctc ttcgctcagc gccggactgt tgagccattt
     5941 ttcataagtt tcccgccagg tcattgttct tcctccgttt catcgtcgct ctcccggatc
     6001 agctcgcagg cgtcctccca caccgaggcg ggaacaaaaa tatcaacgcc cgagccgctg
     6061 atgccgagga tcagcttgcc gaattgtccg tcgttggggt attggcgcag gcacgggatg
     6121 ccgaacgcct ccagacgcgt gacgagcatc gtctcgtcca tgtcgatgcc cttgcagtgg
     6181 cagagataca ccggcggctc cggctggccc gcttcgtcct tgggccagcg ctcgtaaaga
     6241 tcgccgggga gctgacgccc ccactccgcc gatgcttttc cggtttcttc catgatgtct
     6301 ccccgcgggt cgccccgcct gtttttattt tttttattta tgatatttcc cgccctcgtt
     6361 gatgttgaag gcgcggtaaa tctgctccag aaccataacc cgcgccagat ggtgcgggaa
     6421 tgtcatgcgc gacatggaaa ggcggacgtc cgcggcgcgc tttacctcgt ccgacagccc
     6481 gtccgagccg ccgatgagaa agcacacgcg gctttttccc tgcacggcgc actgcgccat
     6541 gagcgaggcc agctcctcgc tggaacagct ctttccctcg atgcacatgg cgacgacgaa
     6601 agcgccggag ccgatgcgcg cgagcatcgc cgcgccgtcg cgctttatgt cgccggtctc
     6661 cggaagctcg atgatctccg gcgcgcagta gcgctgcagg cgtttgttgt attcctcgca
     6721 cgcggcggca taaaagctct ccttgagctt gccggtgcag atcagttgga cgcgcagcac
     6781 ggcgttacct ccagctccag atcgccctcc gccggggcga cgcgcagcgt caccgcggaa
     6841 aagccctcgc cgtcaagcgc ttcgcgcacg gcccgctccg cgaggccggg gcggttattc
     6901 tcccggctca gatggccgag cacgagcgaa cgcaccccgg tctttgccag agcgcaggcg
     6961 agctgcgcgc aagcctcgtt ggagaggtgg ccgcggtcgg acaggatgcg gcgcttgaga
     7021 tagacggggt agcgcccggc gcggagcata ttgacatcgt ggtttgcctc gatcaccgcc
     7081 gcgtcgcacc cggcgagcgc gtcgagcatc tccgccgtca cgcagccggt gtcggtgcag
     7141 acgccgaaac gggcgtcctt ccccgtcaga acatatccta cgctttcggg cgtgtcgtgc
     7201 attgtcggaa aagcggtgag cgtgaaattc ttcagcgcga cgctnnnnnn nnnnccccga
     7261 ccgggatcgg aacgatgcgc ccttccagcc ccgccgcggc gcgctcgagc gcgtgggcga
     7321 cggtctgcgg cgcatagacc ttcacatttt tataatactt cagcagcatc ggcagaccgg
     7381 cgacatggtc ggagtgctcg tgcgtgatga gaacggccga aagatcgtcc ggcgtgagcg
     7441 ctcgccgcgc aaggccggtt ttgaggcggc gcagggaaat gcccgtgtcg agaagcaccg
     7501 ccgcacgtcc ctcggtgaga agatagcagt tgccgctcga gccgctggca aaggatacga
     7561 aagtcatccc gcgacggcgg taccccgctc cgtgccatcc ggctcatcga tgatgcggat
     7621 gtccgcgcca agaagacgca gcttgccgac gaagttttcg tacccgcgct cgatgaactg
     7681 gatatcctcc acgagcgtga ctcctctggc gcagaggccc gcgatgacca tcgccgcgcc
     7741 cgcgcgcagg tcgcacgcgt ccacggaggc gccgcgcagc gtttccacgc cttcaacgac
     7801 cgccacgcgg ccgtccacgg tcacggccgc gcccattttg cgcagctcgt tgatgtattt
     7861 ataccggtga tcccaaacgc cctcggtgat gacgctcgtc ccctccgcca ccgcgagcgc
     7921 cacgccgatc tgcggctgca tatcggtcgg gaagccgggg tacggctggg tcttgacgtt
     7981 ggcgcggcgg agacgccccg tcgagcggac gaggacggat tcgccgtatt cctccaccgt
     8041 gacgcccatt tcgcggagct ttgcggtgat gcagtcaagg tgcttgggaa tgacgttttt
     8101 gatcaggact tctcccccga cggcggcggt ggcggccatg taggtgccgg cctcgatctg
     8161 gtcggggatg atggtgtagg tgccgccgtg gagttgctcc acgccgttga ccttgatggt
     8221 gtccgtaccg gcgccgcgaa cgtccgcgcc catggagttg aggaagttgg caagatcgac
     8281 gatgtgcggc tcgcgcgccg cgttttcgat gatcgtgcgt ccctcggcgt gcacggcggc
     8341 gatcataatg ttcatcgtcg cgccgacgga gaccttatcg agatatacgc tcgcgccgcg
     8401 cagccgtccg ccggggacgt gcgcggtgat gaaatcggaa atatcgacct ccgcgccgag
     8461 ggcgttcatg cccttgacgt gctggtcgat cggccggacg ccgaagttgc agccgcccgg
     8521 catcgtcgtg cgggcaatgc cgaaccgacc gagcatcgag ccgacaaaat agtacgacgc
     8581 gcggatcttg cgcatgcggt catagtccgc gcccatccag gtggcgttcg tcgcgtcgat
     8641 ctccacggtg gagcggtcga tctgctcgac cttcgcgccg agcgattcga ggatgagcag
     8701 cagcgcctcg gtatcgctga tctggggcag gttttcaatg cggcatttgc cgtttaccat
     8761 gagcgccgcc ggaatgatgg ctaccgccgc attcttcgcg ccgctgatct ccacttcgcc
     8821 gtgcagacgg ttgccgcctc tgattctata tctttccaag tgttgactcc tcctgacgcg
     8881 catccggggt cggggtcacc ctttgcccat agatacacgg caattttatc atagcgcgga
     8941 gaaaaacgca agtcttactc cccggcgagg atgtgctccg cctgcagcgc cgcaatggcg
     9001 gttttgccgt cggagatctc gccgcgcttt accatggaga ggagctccga aaacgggagc
     9061 tttacgagat caaggaactc gccctcgtcg ggatg
//
LOCUS       DAWAAE010000081         8789 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3588_length_8789_cov_2.672659, whole genome shotgun sequence.
ACCESSION   DAWAAE010000081 DAWAAE010000000
VERSION     DAWAAE010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_8789_cov_2.672659"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1854 a         2573 c         2594 g         1768 t
ORIGIN      
        1 cgccgctgcg cttctggccg atggcgtcga tctcgtcgat gaacacgatg cacggggcct
       61 tctccttcgc ctggctgaaa agatcgcgca ccttgctcgc gcccatgccg acgaacatct
      121 ccacgaactc cgaaccggag atggagaaga acggcacgtt cgattcacct gccacggcct
      181 tggcaagcat cgtcttgccc gttcccggag ggccgacaag gagaatgccc ttcggcatcg
      241 acgcgccggc gtccgtgtac ttgctcggat tatggagata gtccacgatc tcggcaaggc
      301 tttccttcgc ctcgtcctcc ccggcgacat cgtcaaagcg gatgccgtgc gtggactgga
      361 cgtagatctt ggcattgctc tttcccatgc cgaaggacat gacgtctttg ccgcccatct
      421 gatccatgag ctttttccgc atgaactggc cgaaaataac aaagatgacc agcggcagaa
      481 tgagcgttaa gagatagctc agcaccggcg acatggtctg ctcaatatcc ttggcaaatt
      541 ccgcgccgga atcgtacagg cgctgcgtga gcgtcggatc gtacataaca ccggtcacat
      601 agatcgtatt gtcgtccttg tcggtaaata tgatctccgt gtcgctcaca tcgaccttgc
      661 cgatcttctt ttcggcgatc atgcgcataa aggtgctgta atccgtctgc tccacgtgct
      721 gccggttcag aagcggcgcg acgaagatgt tgaaaagaag cagcgcaatg agcgtaatgg
      781 agtaataata cgccaacggt tttttcgggt ttttgacttc tttcattgtc gaacctcctt
      841 ggtgatttta tgttatatga agctgtttta acggatcttc cggcattcga gataatagcg
      901 cttgtcccgt gcgttgccca tggaaaagct ctttttcggc agagcgccgt tcttgagtac
      961 ggacaggaat agaccgctct tttcgaccgg cggcagcagc accgcagcgc agttcggctt
     1021 ctcgccaaga gagcgcgccg tgtcgtcgcc gtggatatag tcgatatagc cgccgtgcgc
     1081 ggcgatatag gcgcggcaga agctctccgc cgccgcgaca cgctcaccaa gcgttttgtc
     1141 ctctgcttcc cactccgtcc ggcgcgctgt aaatttcgcg gcaaaagccg acgtatcggt
     1201 atcgaaaagc acgcggtgga tcggctcaaa atcaagcgac ggctcatgga tgttctcgat
     1261 ctccacgagc gcgaagcggg cgggatcggt ctcccgctcg ttttccggca gagtttcgcg
     1321 cttttcctcc cagaaccgct tggcagcggc gagcgagtgg ttgccgtcgc cgacggcaaa
     1381 ggtgagcttg ccgttttccg ctgcgtcacc gtatttttcc cgctgcaatg ccgcattatc
     1441 gagcgcgtcg agcgccgact gcaccgccgc tttcgtctcc ccgctcacgc gccagccggc
     1501 aatgtggccg ccgccgagca taagatcaaa atcgtagacc ttttccagtg tgtccttcgc
     1561 cttttccgcg agggacagga cactgtctgt ccggtcgtcc atcagaagga gcgtatgcgg
     1621 catttcgagc ggcgcgccgc ggcggatgtt cacgcgcggc ggcagacgct cctctaccgt
     1681 gccctccgtg gagcggacgc tcgtacgctg cgttccggta aaatcgtact gctcaagatc
     1741 gagcgccgca acgaggccgc agcggacgcg tccgtccgac agcgtgcgct ctacatagat
     1801 aaagctgtcc gggaccgtct gaaaaacacc ctccgcgaga tagcggcgca tcgtatcggc
     1861 aatgcgtccg tccgcgccgt cggcgcgcgc ggtgtgcagc cacgcctccg gcagcatgag
     1921 agaaagcgtg ctcggccttt ccgccgcacg cgcttccgcc gcggcccagt attccggttc
     1981 ggacgtaaac tggtcgcacg cgatcacgct ccacgcgccc atatcctcgg cgcgcgggag
     2041 aaggatatcc gtacttgtaa cgatatgcat aggtgatttc tccttaataa aagagacccg
     2101 tgggactata tagtggataa tacagtcccg cgggtcgaat gtcaatcatg taccgagata
     2161 ggcctttttt acggcctcgt tgttcagaag atctttgcca gcgccggaga gcgtgatgca
     2221 tccggtctcc atgacgtagg cggagtcggc gaccttgagc gccatgttcg cgttctgctc
     2281 gatgagcaga atggatacgc cctggtggtt gatctgacgg atgatctcaa agatatcgcg
     2341 cacaacgagc ggcgcaaggc cgagcgacgg ctcgtccatc ataatgagct tcgggcgtgc
     2401 catgagcgcg cggcccacag ccagcatctg ctgctcgccg ccggaaagcg tgccgcccgc
     2461 ctgccacgag cgctcgcgca ggcgcgggaa aaggtcgtat acccactgga tatcgccgaa
     2521 gatgtcgtcg ctgcggagat acgcgccgac cttgagattt tcgagcaccg tgagatcggg
     2581 aaaaatgtgc cgtccctccg ggacgagcgt gatgcctttt ttcacgatgg tcgtggcgtc
     2641 gcgcccggtg atgttgtcgc cgcggaagtt gatcgtgccg ccggagggct ttacaagccc
     2701 ggcgatcgtg cggagcgtcg agcttttgcc cgcgccgttc gcgccgatga gcgttacgat
     2761 ctcgccttca tgcacatcaa aggaaatgcc cttgacggcc tcaatgccgc cgtagttcac
     2821 gctgagatcg tgaatggaga ggatctcgtt cattcttccg ccacccccag atacgcttca
     2881 ataactttcg ggtcatcctg tacaacggcg ggcgtgccct gcgagatgag tctgccgaaa
     2941 tcaaccacat agatgcggtc ggagaatttc ataacgagct ccatgtggtg ctcgatcata
     3001 aggatcgtga gatcgtattt cttcttgatc tgcatgatga actggccgag ctcttcggtc
     3061 tcctgcgggt tcatgccggc ggccggttcg tcgagcagga gaagcttcgg gtcggtcgca
     3121 agcgcgcggg cgatctccag ccgccgctgc aggccgtacg gcagagacgt tgcaagatcg
     3181 ttgcgatact gcgcgaggtt ctgctcttca agcagctcca tcgtctcctt ctccatgcgc
     3241 cggtcctccg ccacggagat gcggagcgcc gcgctgaaaa tgttctgctt cgcgcgcagg
     3301 tgcttggctg taaggacgtt ttcaaacacc gtcatggatt tccacagtcg gatgttctgg
     3361 aacgtgcggg caatgccgag ctttgtgatc tggtcgggcg tcgggtcgag aattttatcc
     3421 ttgagataca tctcggcgtt catgccctga tattccttct gcatcttccc cgtcggatgc
     3481 gcggagacca tgaggttatc ctcaaaaaac accttgccgt ttgtcggctg gtatacgccg
     3541 gtgatgcagt tgaaggccgt cgttttgccc gcgccgttgg ggccgatgag cgcgacgatc
     3601 tcgcccttat cgacgttgag cgagagattg ttcaccgcca cgacgccgcc gaactgcatg
     3661 gtgacgtttt ccatgcggag cattgattca ctcatggttt tcacctccgg agaccgcagc
     3721 cttttttccg ccgaagagct ttttgcagcg cgccgcgatg cggtcccagc tgaactcctt
     3781 atcgcccata atgccggtac ggaagaacag caccacgacc atgaggatca cggagaacac
     3841 gaccatacgg aagccggtct tgagcagggg cacgcggaaa tcgccgatgt acgttgtttt
     3901 gtccagaaaa cgcagccacc attcgcggct cgcaatgacg agaaacgccg aaataacgct
     3961 gcccgtcacg ctgcccatgc cgccgatgac aacaatgagc aggataaaat acgtcacggc
     4021 gatggtgaac gttgtggaag acaccgcgcc gaggaacatt gccatcagcg cgccgccgat
     4081 ggccgcgaag aacgagctga tgataaagct catctgcttg tgcttggcaa gagcaatgcc
     4141 cattgcctcc gctgcgatct catcgtctcg gatggccttg aacgcgcggc cataggaaga
     4201 gttgatgata aggacgatca gcgtgatgca aacggcggca acgatgaaaa tgccgtagta
     4261 ggacgagaag ccggggatct tcgtcagccc aagagagccg ttcgtaatgc ggttgagcgg
     4321 cgaggaggcg acaatgatgc ggacgacctc cgcaaagccg agcgtcgcaa tggcgagata
     4381 gtcgcttttg agcttgagga ccggcgcgcc gatgagagcg gcaaagagcg cgccgacaac
     4441 gccggcggca agcagcgctg cgatgatcgg cagatggaga tcggcaagcc agtcggcaac
     4501 gccgtaaaga tagtacacgc cggggcgcgc cgccgtgggg atcgtgaaaa tggcgtaggt
     4561 ataagcgccg agcgtcataa accccgcctg accgagagaa aagagccccg tgaaaccgtt
     4621 gcagaggttc atggacaccg caagcagcgc atagatcgcg ccgagctgca aaaccgtgcg
     4681 gaacatcgac gtacgcggaa caagctcgcc ggcgcagacg atgaccgccg cgatgcagag
     4741 aaaaccgacg gcggaacaga tcgttttttt cttttgactc atgctcacac cttctctttc
     4801 agcttctcac cgaacaggcc ggtgggacgg acgagcagta cgaggatcag cacgacgaac
     4861 gtgaaaacgt cgctgaactc ggaataggcg ctgcccttga tgaacgtttc gcaaagaccg
     4921 atgaggaagc cgcctaccag cgcgccggga atggagccga tgccgccgaa caccgcggcg
     4981 acaaagcact tgagcccggg cagagaaccg gccagcggat acaccgacga gcgcgggatg
     5041 aaatacagca ccgaaccgat ggcggccagc gcgctgccga cggcgaacgt gaagctgatg
     5101 acgcggttga tcttgatgcc catgagacag gacgtttcaa aatcacgcga taccgcacgc
     5161 atcgccatgc cgatcttcgt cttgcggatg aggaacgcca gcgccagaac aaggatcacg
     5221 gtgaggatgg gcgtaatgaa cgtaacgagc gtcgtagaga tctcgccgag cttcaccgtt
     5281 ttactgagaa agcccatctc cgcatacgga tacgactttg gctgcgccgt gaagagatag
     5341 gtggcgaagt tctgcagaaa gtacgaaacg ccgatcgccg agatcatgat ggacatacgc
     5401 ggcgcatcgc gaagcggctt ataggcaatg cgctcgatga atacgccgag cagcactgtg
     5461 aacagcacga cgagcagcag tgatatgtac catgggatga gcagatccgt catgcagatg
     5521 atcataaaat aacccgccat catgaagatg tctccgtggg cgaagttgat gagtcgaaga
     5581 ataccgtaaa ccatcgtgta accgatggcg atcagggcat atatactgcc gatggaaata
     5641 ccgttcagac agttctggag aaatgccgtc atagagcttg cctcctcttt cgggttgccg
     5701 atgcgacggg gtaaaagcga ataaagagaa tcggcggggg gacccgccga ttcaaagtgt
     5761 ttatgcggat agaaaatcag gcagcggggg taaaggtgcc gtcggtcttc tgcgtgccga
     5821 cgaacttgaa agcgccgtcg gacatgatct tgatgtaagc catatccttg ttggcgtcgc
     5881 cgttctcgtc gaaggagatg ctgccggtca cgccgtcaaa ggagacggag gcaagcgcgt
     5941 cgcggaccgc ctcgccggtg atatcaccgg aaacattctt gagcgcctca atgatcgcca
     6001 tgtaggcgtc ataaccaagc gcggaaaccg cagcgaccgt gtcgttgttg ccgttgagct
     6061 tgatcgcttc ggcatcgccg ttcagatatg ccttgaagcc ctgcacgaac tcggaagcat
     6121 cggcgtcgtt ctcgtcaaag aaggtggaca gcgcaacgcc ctcgcagttt tccacaccgg
     6181 cgttttccat gatggtagcg ttctcccagg tatcgcccgc catgatgcga gccgtgatgc
     6241 ccatatcacg cgcctgcttg atgatgatgc cggcggtcgt aatggacgac gggatgaaca
     6301 gaacatcggg gtcagccgcc ttggccgcgg tcaggatcgc gttaaagtcg gtttcgttgg
     6361 tgttgtaggt gccggtgtaa acaacttcgc aaccgtccat cttggcaaag gcatcggcga
     6421 agtagttcgc aaggccggtg gagtagtcgt cgccgttctg catgatgacg gcggccttct
     6481 tgcagccttc gttgttggcg tagctcgcca taacggttcc ctggaacggg tcaaggaagc
     6541 agacacggaa gtagtaatcg ttgccgagcg ttacctgcgg gttcgtgcag gagccgccga
     6601 cggcggggat cttcgcctgc tcaaacgtgg agcctgcggc gatggatacg ccggagccgt
     6661 aagagccgat aacagcaata acgccgctgc tcacgaggtt ctgcgcggcg gtaacggccg
     6721 cggtcttgtc ggactggtta tcgacctcaa cgagctcaat cttatattcc ttgccgttga
     6781 ccgtcacact ggggacgacc ttgttggcat agcgcgcgcc gagcacttcc tgcatgccgc
     6841 cggcgccgtt ctcacccgtg gcgggctcaa aaatgccgat cttcagtacg tcgccttcaa
     6901 cggcggccgc cggggattcg ctgctgccgc agccggcaag cagagcgcag atcatgcatg
     6961 cgcaaagtac gatcgctaaa atctttttca tggtttttct ccctctccgg aatacgcata
     7021 gtccgtatgt cacggacaaa tgtgtttcca tttcaaatat gacggtagta taataccgct
     7081 gggagagaaa tgcaaccctt tttttcgcta aatcgcaaaa aaatttcaaa cacactcttg
     7141 gttatcgtgc acggaattcc ggttgttccc tacggaacag aagggcgtgg cgcggtaatc
     7201 gccgtccgcg gcctgcaatg cgcggttttt ccagccaaga acatagaggt cgtgcgcgcg
     7261 gctcccgagg tcgaacaggc gttggatgct ctcattttcg tctttgactc gtgcactttt
     7321 gacgacaaca ggaagagcgg cgctcgtttt catctcccgc agcagcgccg cgccgcgcgc
     7381 atcaaatgcc agcacacgga tgtacggcgg tatgccgttc gcgtccccgg ctcgcacgcc
     7441 gagcgcggcg cacatcacca tgcggcgcag ccgcgcgtgg gcgtaccgct ttgatttggc
     7501 aagatcggcg atctcttccg gggagcttcc gcggcgcgca gcttcgtaaa gccgaaaatg
     7561 cagcccctcc gccgcgtccg gcacggcggc aaagtcctcc ggcgttttct cccgcagccg
     7621 ggaaagatac gccgttcgca gactctccgg agagacaaag ccgcgtccgg cctcatccgc
     7681 acggcgaagg acctcccaca cctccccggg gacacgcccg gcaaggctgt cccccgcgcg
     7741 aagaagcgcg cggagctcgg acgcgctgcc cgcgccgtcg tggatcgagc cttcgcgccg
     7801 gacgggagta taatcaaata tatagccgag cttcgccgcc gcgcggatgt attccacggc
     7861 cagaagatcg ttcggcgtat cgagcaccgc cgcgctctcg ccgagaagag cacgcgccgc
     7921 ccgtgcgcgc gccgcggcaa acggcgttcc gccggtgagt tcgcgcttca gcgcttcggc
     7981 aaattccggc gtgtcgagca ccgccgccgt cttttcgagc gcggagagat cgccgctctc
     8041 gctgccgaag gagagtgcgt cgataacgcc cgctgcaccg agcagcccca cgccgccgcg
     8101 ggcgaagctc tcggcagagg aaagactcca cggcagcggc agctcgatca cgagtgacac
     8161 gccgccgcgc accgcagctt ccgcgcgggt gaatttatcg taagaggccg cctcgccgcg
     8221 ctgcacaaaa tcgccggaca tgaccgcggc gacgggcgtt tccgcgccga agatctcgcg
     8281 tattttccgt atctggtacc ggtgtccgct gtgaaacgga ttgtattccg caacgatccc
     8341 gaccgctttc atataaatcc atcctttttt actcttgcgt tttgatgaaa agaacgctaa
     8401 aatagatatt tgcaaaacaa tgataccctg acgcggtaaa attttcaact atggaaagga
     8461 tccgaaccaa tgaagattct ggttatcaac gccggcagct cttcgctgaa gtatcagctc
     8521 atcgacatgg agaacgagtc tctgctcgcc aaaggtcttt gtgagcgcat cggcatcggc
     8581 ggccgtctga cccacaagcc ccagaacggc aagcccgttt acgaagcgga cgttccgttc
     8641 cccacccaca aggaagcgat cgaggccgtt ctcgaaaagc tgaccagcaa ggaatacggc
     8701 gttgtcgaga cgatggcgga gatcggcgcc gtcggccacc gtgtgctcca cggcggcatt
     8761 gagttcaccg agtcctgcgt cgtggacga
//
LOCUS       DAWAAE010000082         8737 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3623_length_8737_cov_2.763649, whole genome shotgun sequence.
ACCESSION   DAWAAE010000082 DAWAAE010000000
VERSION     DAWAAE010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_8737_cov_2.763649"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4020..4029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4845..4854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1883 a         2545 c         2447 g         1842 t
ORIGIN      
        1 gggctacgat atcatcgatg tggacgagcg catcaagctt gaggaatacg cgacggcgta
       61 taaggcatat ctgaacgccg cgcgcaccga gcgcgaggcc gtgaccgagg ccatcgcgca
      121 ggccgaagcg ctcggctttg tgccgtatga gcgcggcatg gtgctcgagc cgggcacaaa
      181 gatctataag agcgtgcgcg gcaaggcgct catgctcgcc gttatgggcc gcgaaacgct
      241 cgaccatggc gcgaacatcg ccgcggcgca cgtcgattcc ccgcgccttg acctcaagcc
      301 catcccgctc tacgaaaagg acgagatctg ccttctcaag acgcattact acggcggcat
      361 caagaaatac cagtgggtcg ccatcccgct ggaaatccac ggcgtcgtga cgaagaagga
      421 cggcgagacc gttgaggtcg tcatcggcaa ggatcccgag gatccgaagt tcacgatcac
      481 cgacctgctg ccgcatctcg caaaggacca gatggccaag cacggctcgg acatcattcc
      541 gggcgagtat ctgaaccttc tcgccggcag cgtcccctac gccggggagg gcaccaagcg
      601 cgtgatgctc gcggtcatgt cgcttctgaa cgaccgctac gacatcacgg aagaagactt
      661 cctctctgcc gagctggagg tcgtgccgag ctttgaagcg tgcgacatcg gcgtggaccg
      721 ctcgttcatc ggcggctacg gtcaggacga ccgcgtgtgc gcctttgccg agctcaaggc
      781 cattttcgac accgagacac cggatcgcac cgccgtgtgc gtccttgccg acaaggaaga
      841 gatcggctcg atgggcgttt ccggcatgca gagccgcgcg tttgaaacgt tcatggccga
      901 cctctgcgag cagcaggacg tgcatctgcg cgactgcttt gaaaacagct tctgcgtcag
      961 cgcggacgtc tgcaacgcct acgacccgac gttctgggag gtgagcgagt accccaaccg
     1021 caacggcgcc cgcgccaact acggcgtcgg catctgcaag ttcaccggct cgcgcggcaa
     1081 gtccaacgac gcctccgccg aggtcgtcgg caagctgcgc aagatctttg ccgacaacgg
     1141 cgttgtctgg cagatgagcg agctcggcaa ggtcgatcag ggcggcggcg gaacggtcgc
     1201 gcagtatatg gccaaccgca acatcgatac catcgacgcc ggtacgcccg tgttctcgat
     1261 gcacgctccc tttgagaccg tgacgaagtt tgactgctac cagacctaca aggccatgtg
     1321 ggcgttctat aaggacagat aatttctcat cctgctccct atttgcggaa gtgtcctcct
     1381 tccttcccgg cgctttgcgc cgatccacct cccgcaaaga gggcggctca cgtcaaaaga
     1441 taccggcggc tatcggaaac gatagccgcc ggtattttgt gcttgattat tgaataagtc
     1501 cctgcagcag actgttgccg ccggcactct gcgcctgacc gctgctcagg cagaagatca
     1561 cgtcgtccac atcgtagtag tccacaaact tcgtcagcgg gcggctgaag tatttgcggt
     1621 agtccatcac atagacatac tgataatgct gcgtgagata atacccgaac ggattaccgt
     1681 tggagttttt gacgagcagg cacgcgctgc cgtcggtgat gtcgttgttt atgaacgtgc
     1741 acagcgggaa gtcgcccgtg aggaagcaca tgtatttgtc gttcccgtgg atctgggtaa
     1801 tgagcgtctg ctcataaccg cggttggaga gcgagtcgtt gcttccctgg ttgatataga
     1861 gatgcacatc tccgggcggg acatagccgt agacctgatc ggccgtgagc ttgccgctct
     1921 ggtgggcctg atagtagaga ctgccgtaaa acggctcctg cacgatctcc tcatactcgc
     1981 tgagcggcac cggctcaaag cccgccattt ttgcccattc cgcataggcg taccacgcgc
     2041 cgagcgccgt ccagtggtgg tcggtgcgga aatagatgta ttcgctgttg tgcggcagga
     2101 gtgagttgta ggtgtccacg cagtagacat tgtcgcccat ttgggaattt acataggcga
     2161 ggatgtcctc ctcgcatgca acgccgatcg attcgcggta ctcacgcggg agcatgagcg
     2221 tcgtcccgtg gaggacgaat acgtcaaaga cgcggcattt cccctccagc agatcggccg
     2281 cgcgcgtcac attggcggcg taggtgtccg aataatactg gctaaaggcc gtgtaggtat
     2341 aggcgctgtc gccgatctgg atgacggcgc caagcgttac gagctcgtcg tccgggtttt
     2401 ccccgcccca ctccggttcc ggcgtaggtg tcggctccgg ggtaagcgtc ggctccgggg
     2461 tcggcgtcgg ttcgcttgcc ggctttgccg ggtcggcgct cgtctccggc gccggcgtgg
     2521 acggaacggg gattttgtct ccggcagagg cattgccgta caccgttacg gaggagtcgc
     2581 cgtacagcgc ttcgagccgc tgcgcggcca tgatccagcc gtcacgcccc gggaacgtgt
     2641 ccgaaaacca gagactgata tcggaaaaat agcttccgtt cgtcacggcg gaaagagaaa
     2701 actccggaaa cgcctccagc gtgcgttttt cctttgccga caccgtcggg cgcagcggca
     2761 gtatccacgc aatgaccgtg agaatgccca gcgtaaggaa gaacggcagc cccggcagac
     2821 gggcgcggcg tttctgtttt tccgcatggg cgcggcgggc gcggcggctc atgattttgt
     2881 gttcatcgtg cataggatcc ctctcagaac cggaagtata ggaacggatt aaagctgttg
     2941 ccgacaaggc aagcggtgct cagaagcagc agcagaaccg gcgcaacgcc ataggcggca
     3001 acggaatagc ttttctgcgc aagggcgctc ccctctccgg cgcgctccca gcgcttcgca
     3061 agatacgaaa acaccggcgt tgccgccgcg gcgcagaaga tgagcagata catatggctc
     3121 tttatctccg ccgtaacggt gaagctcgta aaagcgttgc cgttgccgca gaacatgccg
     3181 cgcacgaccg ccgcggcgag cgggagcgat gtaaacttga agagcaccca cccgaagagc
     3241 accgcaagcg ggagcgtgat gtgggaaagg atcgccgaga ccgccttcgg cagcttgtcg
     3301 agcagcttct gccagaacag ccggtcgagc atgagcagaa caaagtaata aagcccccag
     3361 agcacaaagt tccagctcgc cccgtgccag aggccggtaa gcgcccagac gatgaagaga
     3421 ttcagaaacg cgcgcgctct tcccttccgg ttgccgccga gcgggatgta cacatagtcg
     3481 cggaaaaatg tgccgagcga catatgccac cgccgccaga actccgtagc cgtgcgggat
     3541 atgtagggat agttgaagtt ttcgagatag tgcagcccga tcatctgtcc catgccgatg
     3601 gccatatcgg aataggcgga aaagtcgaaa tagatctgga acgcatacgc aattatgcca
     3661 atccagagcg caagcgccgg cgtgttggaa agcgccgcga ggttttccgc aagctgcgag
     3721 gccgaaccga tggcggcgtc cgtgagcaga agcgaatcgg caagcgcgcc gcaggcattt
     3781 gcgagcaccg ttttctttgc aaggccaacg gcaaagcgcg tacagccgcg cgcacggtcg
     3841 gtcgtctcgt cgcggccatc gaagagttcc ttcgcgatgg tcttgtaccg gacgatggga
     3901 ccggcgatgc actggtggaa gagcgcggcg tagagcagca catgccagta tttatcctgc
     3961 gcctcggcgt cgccgcggta cacatccaca acatagctca gaagctggaa cgtatagaan
     4021 nnnnnnnnnc gcgatacgct gcacgaacgc cggtacgtcc ccgaagatcg agcagaccat
     4081 gcccgcgtat ttgaaaaacc cgatcaggcc gagattcacg acgcacgcga gcgcgaccca
     4141 gagcctggcg tttttctctt ccctttgcgc tctttgtaca cgcagagcaa aataccagtc
     4201 aaaaagcgcc atcgccatca gcagcagcac atacaccggc tcgccccacg catagaagat
     4261 cagcgagaaa acgagcaggc tgatgttctt tgcccgcagg ctgcggcaga gcgcatacac
     4321 cgccaggctc agcggcagaa atgcatagag aaatatcagg ctggaaaata ccatacggaa
     4381 acacctttgc ggcaaaattt cagaatagat tataggtaaa aattcccgtt ttgtcaattt
     4441 ccacaaataa agaatgtata cagatgcgta cggcgcggca tacttctccg gaggagggtt
     4501 gccgcatgga ggaaagagaa caggaagccc tgcgcgagct tggcgccgca cgggtcggag
     4561 agatccactg catcacgatc atcggacagg tcgaaggaca tgtgatgctg ccgccggaca
     4621 cgaaaacgac caagtacgag cacatcatgc cgctgctcgc cgcagtagag gaaacagagg
     4681 atatccgcgc actgctcata ctgctcaaca ccgtcggcgg cgacattgaa gcggggctct
     4741 gccttgccga gatgatcgcc ggaatgagta agccgacggc gagcgtcgtt ctcggcggcg
     4801 ggcattccat cggcgtgccg ctcgcggtag cggcaaggcg cagcnnnnnn nnnncagacc
     4861 ggtcgttcat cgtgccgagc gccgccgtga cgatccaccc ggtgcggctc acaggcgttg
     4921 tgatcggcgt gccgcagacg tacgattata tttcgcgcat cagcgatagg atcgtccggt
     4981 tcatcaccgg ccattcccac atcacgccgg agagattcac cgagtatatg atgcgcaccg
     5041 gcgagctgac gcaggacatc ggcagcgtgc tcgacggcga ggaagccgtc cgaagcggac
     5101 tcgtcgacca gctcggcggg ctttccgacg cgcttgaata cctgcaccgg cgcatagaaa
     5161 aagagaagtc attttgactt ctcttttgta aaatcatcaa atgaaatttt tctatgtgta
     5221 gaaattcgct ttctaaataa aggattaata catccgaatg acaatccgat aatcagggtc
     5281 aacagccctt gcaactggct gaaaaagttc ttccagcttt ttctcagctg tatcctccgc
     5341 tttttctgtc aattcatccg cctcggcagc cccttttagc acgctcaccg cttggctttc
     5401 aatgtaaaac gcttcttcaa gcgtcatttt tatatcgcca aatgcgagga gccctttgga
     5461 agtttcatca aggataaagt cgttaggatc gactatcaaa tcgtatacct caggacgcgg
     5521 aatatatatt gtaacagttt ttgtgtcaat atccacagca acatcgtttt cgcgcatctg
     5581 attaagattt acagtataaa ttgcttttcc gaagtatgta atgtttttac tttttttgaa
     5641 aattccccaa tcaaacaagc cagttttttc caatgttgaa tgcgcggaaa gctcctgttc
     5701 cataacgatc aaattggcct gttcgttggc ctttccaagg ataacctccg taaatcgaac
     5761 ttcaatggag cggtcgggaa ttgcaacgat tttagcctct cgatagtaat acccacctac
     5821 tgccgcagca actaacatca tgcaaaaaac aagaaatcca ataaagtaaa acataattga
     5881 ccttttctta ttttttgctc tctccagagc tgcctcgtct atgtagtgat actcgtcacg
     5941 tctgttgatt tgattgttga tatttacatg attttccttt ttctctttat actctacgcg
     6001 aacgactcca tcgtctagat gaatcctgcc accacaatac ttacacagaa tatcagtgct
     6061 accgtttgcg tcgcagtcca aagccgcgcc acaatacgga cattcaattt ttactgcaac
     6121 catagtatag tccctctgtt ctcctttttt aacatagtat cacagaaggg aactaatgtc
     6181 tacgagagca tttaaaatac gacaaaaatt tctttcagtt tgtatttttt gtattatgtt
     6241 atgccatatt ttactatttc tttcattaac tggaggtaag aaaaaagaga agtcaagctg
     6301 acttctcttt tttgcttttc atgtaaaaga tcaatgacac aatggctaga cccagcagcg
     6361 cgccggcggt gtcgatgagc acgtcccgca cctgactgcc gcggtgggaa aatatttgga
     6421 gcgtttcgtc tatcgcgccg gtcagcgtgc cgagacccca ggcggtgaaa aaccgctttt
     6481 tcccggcgct ctccagccgg aggaacagaa gcgctgcaaa caccgcatac tccgtgacgt
     6541 gcgccgcctt gcggatgaga tggctcgtcg gctgctcggc aaagccgtcg ccgtcctcgt
     6601 ccgtcatata agtgaaagcg tccggcccga gcccgacctt ttccgccgcg gcgttcagga
     6661 agaacaggat atcgtcgctg attttccccg aaagctcgcg cgagacgagg gagttgcccc
     6721 agatgaacac gagcgcgaga acgatcaaaa ccgtcaaaat gcgtttgcgt ttcatttcag
     6781 atctgcggct tctttacgcg gatacgcgca atgaagcaga agcctacgat gaagagcacg
     6841 ccgcccatca cccacggcat aatgctctcg ttcgcgagac ggacgagaga gacgagcggc
     6901 acgatgaccg ccgaccaagt gaccggcagg ccctcgaacg aggtgcggtt ctcgctcgtg
     6961 gtctcctggc gcttctgctc ggtgacgtta aaatacccca gccggatgac ggcgcagagc
     7021 gtatagaaca tcagcaccgc catggcgaca cccgtaatgc ccatcgcgtg gccgatggcg
     7081 ctcggcagga caccgaacga cacgagatcg cacagcgaat cgatctgaat gccgaacacc
     7141 tcggcgtcct tcgtgcgctt cgtggcgcgg gcgattttcc cgtcgaacat atcgcagagg
     7201 ccgcagagtg caaggccgat caccgccggc cagaccttcc cgtccgccgc ggcgcagatg
     7261 ctccccaccg caatgcacag ctctatgtat gtcagaatga ccgatacatt ccagaatcca
     7321 acgaatttct tgctcactgt ccgttctccg tttcaaaaaa atatatctta tcataaagca
     7381 cgcaggaaca tttttcaaga gcgggatttg acgcggcgga cactctctgc tattctgtaa
     7441 gaacacacta tacaacaccg gaggtctttt tatgacccgt tgggaaaata catcggttta
     7501 ccgcgctctc ggcacgctct cggcactgcc ggtgcgcgcg ctctcgcgtc tgccgctcaa
     7561 agccggcaaa cggcgctgcg gtctttcgtt ttcacagtgg tttctcctgc tcggcatggg
     7621 ggcgatgctc tgcgtgccgc acggggcgtg gaacaatctc tatgccgtgc tcttcgcgct
     7681 cgctgcgctc ttagtctact ggtttggatg cgcggcgcga aaggatgcgc cgcgaaacat
     7741 cggagacctc ggccccgccg cgctcggctt tcttttaatg actgttcttt gcacgctctg
     7801 ggcgggaaac aaagcgggca atctccgcgt tgcgctcttt ttctggacgg gctttctgct
     7861 cgcgtatctc acggcggcgc agtttcgcac acggaccgag cggcgcaccg cggcggcggc
     7921 cctatacatc acgctgctcg ccgtttcgct ctacgggctg tggaactatg tcaccggcgc
     7981 ggaggcctcg gacgtgccga tcgacggcga gatgtaccgg cggctcggcg cgacgctcga
     8041 gcacggcatc aactgcggcg aatttctcgc catggcactg cccatcgcgc tcgtctgggc
     8101 gctgaccgcc ccgacgaagg cacggcgcat cgcgctcacc gtcccgcttc ttctcccctg
     8161 cgcggccatt ctgctcacat acgcccgcac cggttggatc atgctcgcgc tcgcgctcgt
     8221 gctgctgctc tggtataaaa agaagatttt gctgcttccg gcggcggcgc tcggcatcgg
     8281 cgctctgttt cttctgccgg agagcctgca ggcgcggttc ctatcgatgc tcgatttctc
     8341 gaacgcttcg gcgtccggac ggtttgtgct ctggagcgag tgtctggccg tgtggaagga
     8401 ccactggctg ctcggcatcg ggctggggcc agagaatttc tccgcggctt acgcccccta
     8461 tgctaccggt cttctcgatt ttcagccgcc gcactccaat atgctgtatc tagaaatgtt
     8521 cctctcgacc ggcatagtcg gcggactttt gttctgcggc tttttcttcg gcattttcgt
     8581 gcggctgcgc cgcgcgatgc gcgccaggcc cgaaaagcgg gaccgctggg aaacgcaggc
     8641 tctcatcgcc gcgctcgccg gagccgcgct cggcggcatt ccggagtatg tctggtttta
     8701 cccgcgcatt ctctttttct ggagcgtcct cttcggc
//
LOCUS       DAWAAE010000083         8603 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3689_length_8603_cov_2.620964, whole genome shotgun sequence.
ACCESSION   DAWAAE010000083 DAWAAE010000000
VERSION     DAWAAE010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3689_length_8603_cov_2.620964"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2795..2804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6894..6903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1838 a         2505 c         2460 g         1780 t
ORIGIN      
        1 tggtcgagcc gtcgcacggc atggagctgg agatgaagca cttgcccata tgcggcagcg
       61 catccagcac cagagcgccg cactcggagg agggcaccgg gcagcagtcc gccggcaggc
      121 cgaagctctc gaccgcgtcg atgtacggca cgcaggtgag ctggtcgatc tcggagacca
      181 ggaacaccgc gaagagctga tacggaatac cgatcaggtc ggggaagccc gccatgatct
      241 ggcccatcgt catctcgtcg aagagaacga ccttcttgtt cagctcggcg ctgttgccgt
      301 tcttcgcgtc gcccttcgcc tgaaggacga ggttttcggc aacgtagcgg aaaatgtcgt
      361 agatcataag gtggctcatc ttgagagccg cgccgtgctg acccatggtg ttcttatcca
      421 tgaagccaac acagcagaga taggacatca tccagcgata gtgcagcacg gagttgagcg
      481 ccggaatgag atccttggag aacgtgccga ggagcatgcc ccaaatgcgc agccattcat
      541 agaaatcgta cgccgtgtcg gcgacaccgc gccattcgcg gcgaacggta gccatagcgc
      601 ccttcttgta gagacggccg agcttctttt cacccttgag caaaagctcg cggcgctcgg
      661 cgcaggacat ggcggcgatc tcggcggctt cctcggcaac gcgcttgccg aaatcgatcc
      721 agccctgttt ctggcgcgcg ccgaaggctt tccagtcggt ggcaaggagc atgtccttgg
      781 cgatatcaaa gacttccttc aggttttcgc cctgcgcctt ccaatccatg ttgttctttg
      841 tgcggaagaa cgccggattg gggtatttcg gagttttcgc catcagttct cccctccctc
      901 tttgcggatg cgcttgatct ccagcgattc aacgaacgcc tgcacgcggg tgcggagctg
      961 tccggaattg ccgttgttgt acagacggtc gaccgaaagg accggccagt tcttctcttc
     1021 cgccataatg tggctgacga gcgcgcgctc aaagccccag tagtcgcagt acttcatctg
     1081 ttcatagatg atgccctcgg cgccgaactc gcgggcaaga cggtcggcgg tgtcacggcg
     1141 ctgctggacc ttttcatcgg agatgtagcg ggcgcattcg gaatgctgca tgtagtgcag
     1201 gcagagctgc gtaagtacgt cgtcggtgtc gttgagctca atgacctcgc ggcccggcgt
     1261 ggagccgaag cagtagcggt cggaaactac gaacgcgccg cagttttcaa tgagcttggt
     1321 gagcatggga tcgtcgatct cgctgccgac gatggaaaca cgggcgcggt accagggctt
     1381 cacatcgacc ttgcgctttt tgatctcggc aagcgtctcg cgcaggtacg gcaggataag
     1441 cgccttcggg caggtgtagg aaaccagatt gagaacgtgg tactcgtagc cggtgatggg
     1501 cggattttcc attttgcgca tctcgccgat ctcggtaagg atgcggcaga cttcgttgtg
     1561 ctcctccacg gccttgcgca gcgcctcgtc cgacgtgtcc gcgccgagct tttccttcat
     1621 aacatcgaga acacggtagc gcatctgctt cacataggac tcgagcgtgt agtcggtgat
     1681 cttgaaggga atgtccgcgt gggtcacgaa gaagttgggc ttgtcgttta cctgcaggat
     1741 ttcaaagtgc tccatagcac ggttcatcat ggagcaggcg tccacgccga tcatggcgtc
     1801 caggaactgg tagccgccct cgatggcgcg ctcggtgagc gctctggcga actcgcaggt
     1861 gtagttcgac atgtagtacg ttgcaatgtc gatggagccg gtgtgcggag cgcggagacg
     1921 gacggagaag cagttgccaa cgttgaggat cggttcgggg acatggtagc aggtgtaccc
     1981 catggccaga ttcccttccg cggctgcctt tcgcacaagc tcgttgtcgg cattatccag
     2041 aagctgctca aagtagatca gatgcttgag atccttcaaa tgtcacaccc ctttttgatt
     2101 tctttctctt gttgtttgcc ctttatttat aagtttaatt gcgcgccttt cagagaatgc
     2161 cgaacttttc cagaacttcc ttcgttccgc ggtacacgct cgactccggc ggaagatcgt
     2221 acaccgtttt gccctggatg tcgttggcgg catggtaatc gtccgactcg atataggaaa
     2281 gaatttcaac gcccggaatc gtgatgaact tgttgagctc cgggtttgtc acgcggttga
     2341 ttaccgcgcc gatgcggtcg tacatgacaa gctcatcggc gacctgcttg atcgttccga
     2401 cgacctgaat gcccttgcgg ctctggtcgg agacgagcag aaggtgcgtg accttctcaa
     2461 gcacgcggcg gttgatctgc tcgataccgg cttcgccgtc gataacaaca taatcaaact
     2521 cgcgggagag catcgtgata acgtccttca gataggcatt gatgcggcag tagcagccct
     2581 ccgcttccgg gcggccgatg gcgagaaagg cgaaaccgtt cttttccacc agcgcgtcga
     2641 agatgcggta acgggattcc tccacgagat ccatcgcttc cttcgtgcgg ccctcttcca
     2701 cgttatcaat gatctgcttg cggatctcgt caagcgtgag cgtgacctcc acgccgaggc
     2761 acgttgaaag accgacggcg ggatcggcgt cgatnnnnnn nnnncgtccg gtttgcactc
     2821 cgtcaggtgg cgcaccatgg ccgccgccag cgtcgttttt cccactccgc cctttccgga
     2881 tacggcaatc acttttgcag ttgtttccat cgcaagtttc ctttcgcgca gtttgatgaa
     2941 tttgtgtgcg tttttagaca cttttacaga ttttaaccac ataatcattg tatcactttc
     3001 tactaaaata atcaaggatt tcttattatt ttttgttgat tatctgtgct ggttgtgtta
     3061 aattgaacat gatgttagtg taggattcac aacggctgtg aacaaatcat taagaaattg
     3121 agaacaatta caaataaagt atgaaagagg gatttggatt tgaaaaccga tgcgttggtc
     3181 gaaagactcg tcaacagcta tggcagctgg gtgcagaagg actgcgaaaa taccgaaggc
     3241 ggtaccggac gctataccag ggagctctgg ccgtatgaca cgatgttctc ccccatccag
     3301 gtcaaccgtc tgacgattaa gaatcgtctt gtgatggcgc cgatgggcaa ctgccagatg
     3361 gcggaagaga ccggccgccc gaacgacaaa atgcgcgagt acttttttgc ccgcgccgaa
     3421 ggcggcgtcg gccttctcac cacgggcctc atccccatca gccaccacat cgactcgtcc
     3481 gtgacggaaa agggcaactt ctcctacttt ccccgcatcg acaacacccg caccaacttc
     3541 atgggctggc gcgatctggc gcagggcgtc cacgcgcgca acagccgcat tttcatccag
     3601 ctcaccgccg gtctcggccg tgtcggcaac ccgcagtgcc ttatgacgaa gtacgcgctg
     3661 cccgtgtccg cctcctggaa tccgaacttc tatattccga gcattccctg ccgtcccctt
     3721 accgaccttg agctggacca gatcgtccgc aacttcggtc agggcgcagc agacgccaag
     3781 acgatgggca tcgacggcgt gtatctgcac ggccacgagg gctatctcat cgatcagctc
     3841 accaacaccg cgttcaacca ccgcaagctc ggcaaatata ccgactggca gctcttcggc
     3901 atcaacatcg tcaaggagat gcgcaagcgc actggtccgt ggttcccgat catgtaccgc
     3961 atcgatctga gctgcgctct cgaagagacc tacggcgagc gcatgggcac cgtttcctcc
     4021 ctcaaaggct tcaaaaacgg ccgttccatt tatgaaacgc tcgactatat ggaaaacctc
     4081 gtcaaggcgg gcgtcgacat gttcgacgtg gacctcggct gctacgacaa ctggtggctc
     4141 ccgcatccgc ccgcgtatat gcccgccggc tgcttccttg acgtggcgaa ggtcgccaag
     4201 gaatacttca aggagcgcgg cgtgaagtcc aatgccggat ttgaggttcc catcgtcgct
     4261 gtcggcaagc tcaactatcc cgatatggat gagaaagccc tgcgcgacgg caagtgcgac
     4321 atgatcatgc tcggccgccc ggtgctcgcc gatcccgact ggtgcaggaa agcctatgcg
     4381 ggcgacgtgg accagatccg tccgtgcatc ggctgtcagg aagcctgcgt caacgagttc
     4441 gtcgagggag gtcacccgca gtgcgccgtc aacccgcgca ccggccatga agatgtgctg
     4501 cccaagcagt ccgcgcccgc tgtaacggcg aagaagatcg gcgtcgtcgg cggcggcccc
     4561 gccggcatga actttgccat ctacgccgcg cagcgcggcc acaccgtcga gatccttgag
     4621 aaaacgagcg agttcggcgg cattgcgaac gccgctgccg ttcccaaaaa taaatacgac
     4681 atgcacaact atgtcgaatg gctcaagcgc cagacgctcg ggcagaaggg cgttgcggtg
     4741 aagttcggcg tagaggccac gccggaatat ctcaaggcgc agaactacga cgccgtgatc
     4801 tatgccgtcg gcgccaagcg catcagcccg cgcattcccg gcatcgacac cgtgaacatg
     4861 gtcgaagcga ccgagcttct gcgcaatccc gaaaagctcg gcggtgcgaa gaaggtcgtc
     4921 gttctcggcg gcggcgcggt cggcatggag accgcctact ggctgagcta cgagcacggc
     4981 tgcaggatca ccgtgatcga catgctcggc aacttcatgg acggcgcctg caccgccaac
     5041 cgcggccaca tcatccacta tcttgaggat aacggcgcga agctcgtcaa ctgcgcgacg
     5101 atcaagtcgt ttgagccggg cagggtgctc gtcagccgca acatctccaa gggcgcgccg
     5161 gacgtttact gcacctggac gccggttctg ccgaagaacg tcgtcaaccc gctcgcgccg
     5221 aagatgggtc ccgaaacggt cgtcgaagag caccacgcgg agctgttcgt caacgcgctc
     5281 ggccgccgtg ccgacgagag ccagtatctc gagggcgtga agcagcatgt cgccaaggag
     5341 atctacaaca tcggcgactc cttccaggct ggcctcgtct ggggcgctac caaagccgcc
     5401 tacaacctcg cgcagtacat ctgatccgat aactgcctaa gcgcataaaa tgacgccgta
     5461 tccggaaaaa ccggatatgg cgtcataatt tatatctgtt atcgggtata aacggaagat
     5521 catttttccc ccgctgcggc gcgggcgtag gcgacgatga tcgccgccgt ttcttccagt
     5581 cccgcggagg aatccaccgt cagctcatag ttttcggcat cgccccagcg gcggccggag
     5641 atgtgccggt aatacgacgc ccgcgccttg tcggagcggc ggatattgcg ttttgcctcc
     5701 cgcagcgtgt ccccgtatac ctccatgaca cgacggattc ggtagtcgag cggtgcgtga
     5761 acgaacacac ggacaacatt tttatgctcc cgcagaacat aatccgcggc gcgccctacg
     5821 ataacgcacg aaccgttgtc ggcgatgcgg cggatgatcc ggtcctgcgc cgcgatggcc
     5881 cgctgaacca cctgcgagct gagatagaga tcacgcatcg cgtccggagc gtttttgtgg
     5941 atatcggaaa tgaattcgcg gtcgagtccg ctctcatgcg cggccagcgc caccatctcc
     6001 ttgtagtaaa acgggatgcc aagcttctgc gcaacgagct ttccgatctg cttgccggag
     6061 gagccgtgct cgcgggcgat ggtgatgatg acgcccggct tcgagggttt gagcgccgcg
     6121 gcgtctccgt catccgcgcg cagcggcttc ggctcgctga catagtcctt tttgaggaac
     6181 tgccggtaga atacaaaccc cacgatgctc gtcaggatct ccgtaaccgg atacgtcagc
     6241 cagaaccggc tcagcccgaa gcgggagaaa agatagccga gcgggacgaa cagcacgact
     6301 gtccgtatca ccgtaagcgc cgagcttttc agacttgagc ccaccgcctg aaagaagacc
     6361 ggaaagatca gcgatgtgac catcgggata aagccgaggc cgatgatgcg gaagccgacc
     6421 gtgccgatct cgatcacgag cgcatcggaa gtgaatgtcc gcagcagctg tgacggcaga
     6481 gaaacgaaga tcagcgtgcc gacggccatc agcgccgcgc cgaacaggac agaggccgaa
     6541 agcgtctttt tacagcggtc gatgtttcgc gcggcgtaat tgtagctgat gaccggaacg
     6601 atgcacgtct gcatcgcgcc gagcgggata aagaagaacg tctgccactt atagtaaatg
     6661 ccgagagcgg tgacagcctc gtcgcagaag ctcgccaaaa tcagattaag gccaaatatg
     6721 taaaaagtgt acgccgactg catcaggata ttcggtatgc cgagacggaa gatctttgcg
     6781 acgtggtgcg gatacgcctt tgccgcgggc gagcggcgaa agccctttct catcacgacg
     6841 agcgccgcca cgatctgccc cgcaacagta gcaacggcag caccggcgat cccnnnnnnn
     6901 nnngccgaaa aggccgaaga tcaaaagcgg gtcgagaacg atgttcgtga ccgcgccgag
     6961 gatctgggcg atcattggcg ttttcatatc gccgatcgat tgcagcacct ttgtccagat
     7021 gctctcgagg aacagcccaa agctgaatac gcatacgatc cggccatata ccgttacatc
     7081 gcgtatcacc gcgtcagacg aggtggatat tctcgcatag gcgggcatga ccgcccagca
     7141 gaccgcagcg aacaaaagcc acagcgcggc ggcgagcagt gttcccacac cggcatactc
     7201 atctgccttt ttcgcctcgc ccaggccgag ctttgccgcc atcaccgtgt tgatccccac
     7261 gcccgttccg acggcgagag cgatcatcaa aagctggatc ggatagatga tggacaaagc
     7321 cgtcagaccg gattccgaat accgccccac aaacagactg tccacgatat tataaagcgc
     7381 ctgaatgagc tgcgcgagca tcaccggcgg cgccagacgc aacagaactc tgctgatttt
     7441 ttccgtccgg aacagttcgc tgtgttccac tttggtatcc ctcgtttttc tcgtttgata
     7501 tcccgcgttt ttcgcgggta ggcggcattg tagccgctta tggaaaattt gtcaatgggg
     7561 ctgagtatgc cgtatagcta tcctgtgtgt tttcttcttt tcaaaccttc cccaagtcat
     7621 tataataaga acgtctgaat aaggaggaat aaaccatgaa taccgcatac tatcagggaa
     7681 accgggaacg gctctatgcc atgatgcagc cgggttcgct tttgatgctt ttttccggcg
     7741 aagagatccg gaaaacgaac gacgaatatt atccgttttt cgcggatcgg agctttgtct
     7801 actttacggg gctgaagtgc aggcaggcgg tgctgctcgc gctgaaagac gccgagggca
     7861 gtgtcacgga gcggctgtat ctgctgccgc cggacgctat tgccgagcgc tggaccggca
     7921 cccggatcaa gccgcaggaa gcggaaagcg tttccggcgt taaggatgtg cgcttcgtcg
     7981 gcgcgctgga aacggatttt tccgcgctgg cgaacagcgg aaactacgga gccgtgtatc
     8041 tcgacctcta ccgcatctcc cccgctgaca ttgaccgccc ggcgcacagc tggcgaaacg
     8101 tgattcagcg ggaatatccg ttcctgcgtc tggaaaacgc caacatactg atccgccggc
     8161 tgcggcttat aaagcagccc tgcgagatcg aggcgctgcg tgaagccgag aagatcacca
     8221 aggcgggcat tctggccatg atgcacgctt cccgccccgg aaaatatgag taccaataca
     8281 aagcggattt tgatcatgcg ctggcgcagt tcggcccgga cggtcccgga tttccctcca
     8341 tcatttccgc cggaaaaaac aacttctgca tccattatta cagctacacc gggcaggcgc
     8401 aggacggtga catgatcctc aacgatgtcg gcgcgcagta cgacaacatg atcaccgacg
     8461 tatcccgcgg ctggccgtgc aacggacggt tctcgcctcg tcagcgactt ttgtacgagt
     8521 gcgtgctggc tacctcggac tatatgtttt cgatcattcg ccccggtatg cccatggaag
     8581 aggtagatgc cacgatccgc cgc
//
LOCUS       DAWAAE010000084         8579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3704_length_8579_cov_3.029798, whole genome shotgun sequence.
ACCESSION   DAWAAE010000084 DAWAAE010000000
VERSION     DAWAAE010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3704_length_8579_cov_3.029798"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1942..1951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6311..6320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1619 a         2674 c         2566 g         1700 t
ORIGIN      
        1 ttttccgcgg ggaatgctct ttttcagcgg cgccgcgcga aggtttcgtc atttttcttt
       61 attatagtct tttcccggcc cggattcaat gtcttttacc cattgcattg tgaaagattt
      121 ggtattataa tggcaaaaac attttccata acaaaacctt tgggaggaga ccgccgtggc
      181 acgctctgcc aatcagaagc tcaagctgct gcttctccgg cagtatctcg agcgcaattc
      241 cgacgaagcc caccctgtca ccacagcgca gctcttggag tatctcgcct cgaagggcat
      301 cagcgccgag cgcaagagca tttacagcga tctggaggcc ctgcagtcct tcggactgga
      361 tctgatccgt gtccgcgacg ggaacatcac ctgctggtac atcggcgagc gggtgttcca
      421 gctgccggag ctgcggctgc tggccgacag cgtgcagtcc tcgcggttca ttacgcgcaa
      481 aaagtcgctg gagttgatcg caaagcttga agggctgacc agcgtgcatc aggccaagga
      541 gctgcggcgg caggtcgtcg tcaaaaaccg catcaaggcg atgaacgaat ccatatatta
      601 tctggtggac gagctgcaca ccgccatcaa cggcgacaag cgcatccggt tccactacac
      661 gggatacgac gggcacggcc ggcgcgttct gcgccgcggc ggcgcgtggt acgacgtgag
      721 cccctatgcc ctgctctggg acgacgagaa ctactacctc atcggctacg acagcgcctt
      781 ttccgagatc cgccacttcc gcgtggacaa gatctccgat ctgtgcaccg ccggagacgg
      841 gcgcgaaggg ggcaaggcct tcgccgcgct cgatatgagc gcgtatacaa gcgcccattt
      901 cggcatgttt tcgggtacgc cggagaacat ccggctcgaa tgccgcgata cgctcgccgg
      961 tgccgtgatc gaccggttcg gcacggacac catgctcatc ccctcgcgcg agggatattt
     1021 caccgtgacc gtgccggtgg cggtgagcgc gccgtttttc ggctggctgt gctcgttcgg
     1081 cggggatata aagctcctcg caccggagag cgccgtggac gcgctgcggg agcatctccg
     1141 gaagttcgcc gaagcgtatc agatttagtt tttttgccgc gcatatctca taaaaaattt
     1201 cttacaagga ggggtaaaca tgagcggctg catcattccc gaggggtaca cgccccggct
     1261 gaacgtctac gagacgcaga gagccatcga atttatcaag aggagctttc agaagaattt
     1321 gggctcggcg ctgaatctca agcgcgtatc cgcgccgctg ttcgtgcgca ccgataccgg
     1381 cctgaacgat gacctgaacg gcatcgagcg tccggtctcc ttcgatgtcc ccgccgtgga
     1441 cggcgcggag tgccaggtcg tacagtctct ggcaaagtgg aagcgctggg cgctgcgcga
     1501 gtataacttc tatgagggca agggccttta caccgatatg aacgccatcc ggcgcgacga
     1561 gccggtgctc gacaatatcc actcggtcta tgtagaccag tgggactggg aaaaggtcat
     1621 ccgcgaggag gaccgcaatc tcgactatct caaggacgcc gtgcggcgca tcgtaacggc
     1681 catctgcatg accggagacg agctggaatg ggaattcccg cagctgcgcg cccatctgag
     1741 ccgcgacgta tccttcatca cgagccagga gctggaggat atgtatcccg atctcacgcc
     1801 ctccgagcgc gagaacgagt acaccaaaac gcacccgacc tgctttatca tgcagatcgg
     1861 caaaacgctc cgctccggca agaaacacga cgggcgcgcc cccgactacg atgactggga
     1921 cctgaacggc gatattatct tnnnnnnnnn nccatgggca tccgcgtcag ccccgagagc
     1981 atggaccgcc agctcacgct cgccgggtgc gacgaccgcc ggaagctgcc gttccacaag
     2041 gcgctgcttg cgggcgaaat gccgttcacg atgggcggcg gcgtcggcca gtcgcggctc
     2101 tgcatgctgc tcatcggctg cgcgcacatc ggcgaggtac aatccggaat ttgggatgaa
     2161 gaaacggtga acctttgcaa ggcccgcggc gtccatctac tgtaaacacg caaacgagag
     2221 ccttgcgctg catggctctc gtttgacggg gacggcagcg cgccgcagcg tactcgcttt
     2281 gctcgcacgt ttgcgagccg ggaagcagtt ttaccagcgt tcacgccgcc ggcaaaaaat
     2341 gttttaaact tttttgcacc tgcaggcgca aactccgcga gacttttttg agcggcgctc
     2401 cgcgcgccgc tcattcttat ccccatcccc tgcacacttt cttttcggag gcatcccatg
     2461 gccgaggtaa acgaaacaaa aaaaccggtg aaaaaccgga aaaagaccgt ctggctcatc
     2521 gtcgcgggcg cggtgcttgc cgtcatgctg ctcgcggcgc tcgcgctctt ctgccccggc
     2581 gtcagccttc tccgcacgcc cgctccggcg gctgtcacgc cgccgcccgt accggaagca
     2641 accgctgaac cgacgccgga ggccactgcc gcgccggtgt acgccgcggg gtatatgcgc
     2701 tgtgaaaacg gccttttcca cggcgcggat acgctgaccg ccggagaggc cgcggacgcc
     2761 gtgcagcgcg ccgccggtat cacgctcgcc gtggacgacg cctctctccc gctcacggaa
     2821 gtatcgctcg cggcgctgct tgacggcgct tttccgaccg acgccataga aaacgccatg
     2881 acggccattg cgatgcacgg cggtgagaac gttacgcgcg ccgaggccgc ggtatttttt
     2941 gaccggctct tctctctgcc cgccgccgat acggagacga cgttttatcc ggacgttatc
     3001 cccggcgaat ggggcagcgg cgacatatac accgccgcgg acggcgcggc cgtatgggac
     3061 gggccggaag gccgccccgc cgaggggtcg gttttgatcg attcgcagct ctattatgcc
     3121 ggagcggacg gctattttct caaaaacgcc tattccggct cgctcttttt tgacagcgcc
     3181 ggacgctata cctccggcaa cgccgagctt gacggctacg ttctcgccgc gctgcgcgag
     3241 gcgaccgacg attccatgac gcgcgacgag atgctgcgcg cgatgtatct ttatgtccgc
     3301 gacagcttca cctatctgcg ccgccattac tacaagaccg gcgacgtcgg ctgggcaacg
     3361 caggaagcgc tcacgatgta ctcgaccggc cgcggcaact gctactgcta cgcctcggcg
     3421 ttctgggccg cggcgcgcgc gctcggcttt gatgcgaaga tcgtcagcgg cacctacggc
     3481 gaggaagaag cgccgcacgg ctgggtggag atattgcgcg gcggcgagcg gtacacctac
     3541 gatgtggaga tcgaaatggc ggtgcggcgc gaccgcggcg cgtccgatct ctatgaaatg
     3601 gacgattcgg cgcggcggcg ccacggatat caggagtttt ccgccacgga cgacatgctg
     3661 ccccgctctc ttgcgggcga gctgctgccg ggctgaaagg agagcacatg aaaaaacgat
     3721 ttcttctctt ttcggcgctg ctctcgcttc tcctgctcac cggctgcacg aagcagctga
     3781 cggataagcc tctcacgctc tccttccccg aggaaaccgc agtattcacc ggcacgcttt
     3841 ccaagggcac ttataccggc accgtgctgg gcgacggctg gcggtttgag ggaacgctca
     3901 ccgccgagtg ccttctcacc ggcgaggggg aaaatgttcc ctgccgcgcc gcgctcttcg
     3961 ggagcgtgga ggacggaacg tacaccggct cgctcgtctc cggcatgccg gacggcgagg
     4021 gcgtgttcac gctcgcctcg ggcgcttcgt tcaccggcga attttccggc ggcacggcgg
     4081 tacgcggcga ggcagccgat ctgccgtgga cgctgctgca cggcggcagc cgcttttccg
     4141 ggacctacac cggcccgctt gagagcgccg gccccgaggg cgaagggctc ttcggcggcg
     4201 agaacgccgc cgggcagcag ttcacctggc acggcggctg gagcggcggc gagaccgcgg
     4261 gccccggcgt tttgacggac gagcactgca tcgtctccgt cgaaggccgc gaaacggccg
     4321 gaagctacgc cggagacggc tgcgacggtc tgccggacgg cgaaggcgtt ttcaccggcg
     4381 agagtgagac cggcgttccg ttcacctata cgggcgaatg gtcggcgggg ctcatggacg
     4441 gcaagggaac gctttcctac gacgcggaaa acctctacac gcgcaccggt acgttcaccg
     4501 ccgggcgctt tacgccggac gggcttgaga cactcgtctc tctcggcacc tgcgagccgg
     4561 aattcacact cacggaaagc cagctcacct tcctttgcga gaccgccgat ctctgggagc
     4621 gcgcagatca tcaggatttc ttcaactccg cttatgcggc gctgcgcgcc aaaaacgttt
     4681 cgatcgcgca gtgtttttcg gacgattcct tccgcgagga gccgtactgg ttggagatct
     4741 acgccatgcg catcatcacg gcgcgcaccg gcgctctcgt ccccggcggc aaggagatga
     4801 cgtgcatctt cgccgttgag cgcaattatc agtacccgtg ctttgtgatc gtccccggca
     4861 gcgttgaccg tctctcgcgt tggaactctt tccacgttta cgccgtgcct atcgccgtaa
     4921 gtccgtatat caacacgctt ggcgaagaga aggaatgcct tgttctcctt gcgggggata
     4981 tatataccgg aatgtaaaaa aatgaccgcc tccccggttc gcctggggag gcggtttatc
     5041 atccctccgt ctcgccttcg gcgagccggc tccctttcac aaggatgctt ttttatgcgg
     5101 aggcggcgtg ctggcggccg gtgtggtcga tgtaggtgtc acccgtgagc aagatctccg
     5161 agatcggcac gatcgtatac ccctccgcga gcaggtattc aatgatagag ggcagcgctt
     5221 cgggcgtatg cagccccgcg ttgtggaaca aaacgatgct ccccggctgc accttggacg
     5281 atacgcgctt atagatctcg cccgccgtca gctttttcca gtcgagggag tcggtgtccc
     5341 actgcaccgc cgtgaggccc atgccgttca cggtgccgat aacgttgtcg tcatattcgc
     5401 cgtaaggaca gcggaatagc gtcgggcgca cgccggtgac ggcctcgatc ttgtcgctgc
     5461 agcggttcac ctcgtcgatg atctgctttg cggtgagctt tgccatgtgc gggtgagcgt
     5521 cggaatggtt catcacctcg tgcccggcgt cgtggagcgc ctttacgctc tccgggtatt
     5581 tgtccaccca ctcgccgacg acaaagaacg tcgccttcac gttgtacttt gccaggatat
     5641 cgatgagcat ctgcgtatcc tcgttgcccc aggccgcgtc gaacgaaagg cttacaaact
     5701 ttccgtcccg ctccacgctg tatacgggca gctcacgttc cgtgaccgcc gcgccgacga
     5761 cgcgcggaga gtatatgaac gcaaagatcg ccgcaacagc aagcacgagc gccgtaccgg
     5821 aagcaaattt attgtgccgt gagagaaagc cgccaatttt acctgccatc ataaatttat
     5881 cccccctcgc ggacatccta tgaacgcggg aggggatgta ttcattgttc gtccccgcgc
     5941 gggaagatca cgttgggaga atagccggta tacttcttat agaccttgtt gaaatacttg
     6001 taatcggaaa aacccaccag ctccgcgatc tcgtagagct tgcgctcgcc gcgcttctgc
     6061 agctccacgg cgcgccgaat gcggtagtaa ttgagaaaat cgtggaacgt gtagcccgtt
     6121 tccgccttga acttggcgtt caggtgcgtg caggatatgt tcagctgctg gctcagatca
     6181 gtgagcgaga tgtgctcggc gtaatgctgc cggatgtact gcatcatggc ctcggcatat
     6241 ttattctgca gcggcgcggg tttttcgtcc ggctccgggg tttccccgct ctgtccgtcc
     6301 ggcgctccgg nnnnnnnnnn cgccgcagac agtttttgag ctcgttcacg tccacgggct
     6361 tgagcaggta ttccgccaca cccagccgga tggcctgctg cgcatagtca aactcgccat
     6421 agccggagat gatgatcgcg tgaaacggac aaacgccgcg accctcgcgc agcatttcca
     6481 gcccgtccat atcgggcatg cggatatcgg tgatgacgat atccgggcgc agcgctttga
     6541 tctgggccag gccctcctgt cctgtggcgg cctcgccgac gaccacgcag ttgacctccg
     6601 tccagtccat tttgtattgc agtccccggc ggatcagatt ttcatcttcc accagcaaaa
     6661 ccttcagcat ccttcgttct cctccacttc ataagggatg gtgagcgtta cagcggtacc
     6721 ctgcccctcc gtgctctcga tctgcacgcc gtagggcgcg ccgtaggtca ggcgaatgac
     6781 cttctggatg ttataaagcc ccacatgagc ggcatattcg ttcgtgagat cgtgctcgaa
     6841 gctctcgcgc agctcggcca gacgcccggc agggatgccc gcgccgtcgt ccgttacgat
     6901 gaagcgcagc gtatcgtccc ggtgctccgc ctgcacgcgg atggagagcg tccgcccggc
     6961 acggtagccg tgaacgatgc tgttttcgat gaccggctgc agaaggagct tcggcacacg
     7021 gcattcgagc agctcctccg gaatatcgaa atcatagagc aggcagttgt tgtaccgcgc
     7081 cttctgcagc agcagatagt cgttcacata ctccacgtct gtttcaagca gcaccttcgt
     7141 gtggccgtaa ttgacgctgt agcgcatgag cgaggcgaac gagagcagca tctccgaggc
     7201 cgtttccgga tcctcgctga tctgatagcg tacggtctcc atgacgttgt agacaaagtg
     7261 cgggttgaac tgctcttcga gatgcttgat ctccatctga cgccgccggt ttaaaagctc
     7321 gtcgttatgc gcgatcagct cccggaagcg ggtatagagc gcctgcgact cctcggagca
     7381 ctgcggcggc agtgcctccg cgccgccgtt tttggaaaga tcctccaccg cggcggtcag
     7441 ctcgccgagc tccatggcgt tctggcgcgt gaacgccttc gtcatggcga cgacgacacc
     7501 ggtgagcagc agaagaagga cgataaaaag gccgccgctg tagagcagca tccgaagctg
     7561 catatccagg gacgtgagcg tatacagccg cagtgcctgc ggccctaccg caccgcagac
     7621 ggcatagtac cggctgccgt ccagcgatag gatgccgtcg tcctcgagag acagcgaaaa
     7681 cttgttgctc ggctgcttgt cctgcgggtc ttcccgcgga tcgagcgtcg tgtagatcag
     7741 gttatcgtac cggtccgtca gcagtacctc ggaatcggta tcacgcacag cgtcccggaa
     7801 gccctcctgc cggagattca gcagaagata tcccgccgcg gccccggtct catccggcac
     7861 ggcgcggcag agcgtataag cgcacgcctg ctcgctgctc accggcgcgg tgcagacata
     7921 ccggagcgtt gccgccggtt cctgcttcat gcgcgtcgca gcgcccgcgg caaacgcgga
     7981 cgaggcgaag atcgactggt tgtgcgcgct gaagttggag ctgagcacct gcatatccgc
     8041 gccgagcagc acgaaatatg cgcgcagctt ctgctgattg gtgaaatcat agagcagccg
     8101 gttggcttcc agacactcgc ctcgatcgcc gcttccgacg gcgcggcgca gcgcgtccgc
     8161 tccggtcaac tcctctgccc cgcggtcata cgccgcgagc tgctgcgtga gcgccgtttc
     8221 gagctcccga ttgtccctcc ggcagccgcg cgcgacaacg gcaaagaagt tcagcgcgaa
     8281 gccgcccaga tacaaaagca ggatcagcgc cagtatggca gccacgtagc cgatgaacga
     8341 gcggcgcagc tgattttgaa acggttcgcg gtgcatggcc gtccctctct ttcccggcgt
     8401 cattttacca tattcttccc tttctgaaaa gggcgaaaag cggtgcgccc attcaggcgc
     8461 accgcacagc ctatcgaaaa agcctcgcag agtttgcgcc cgcaggcgca aataaagttc
     8521 aaagcatttt tgccggcggc gtgtacgtcg gcaaaaatac ttctcggctc gcaaacgtg
//
LOCUS       DAWAAE010000085         8463 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3768_length_8463_cov_2.832659, whole genome shotgun sequence.
ACCESSION   DAWAAE010000085 DAWAAE010000000
VERSION     DAWAAE010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3768_length_8463_cov_2.832659"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5611..5620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1743 a         2408 c         2492 g         1810 t
ORIGIN      
        1 ggcttcgggc tgagcaccgc aggcgcacag gccgatgcac agcgtcagag cgagtagcag
       61 agcaacgaac tttttcatgt tttctttcct ccagtaattt tttgtttttt tactgttact
      121 gtttatttcc gccggcaagc cggaagaaag caaaaagaaa ttgcggaaac cggtcaacgg
      181 cctccgcaaa cgaacgaaaa acaaagcaga agaaaacgct gctttgtccc tcgtcgatag
      241 tccggtcctt tacggtgacc gggtagagac ttcaaaacca tatcatttga tttatatcga
      301 cagatatgga attgtaagga aagcgccgtc agcagaactc gatgccgttt tcctcgctca
      361 tggatttcac acgggccaga gattccacac ggacgccctt gctgcggagc atgtctccgc
      421 cgggttgaaa tgctttttca atacagatgc cgcagccgac gagcgttgcg ccggactgct
      481 ccaccagctc gatgagaccg ctgagcgcag cgccgttggc aagaaaatcg tcaatgacga
      541 gtacgcggtc gttcgggccg agaaaatcct tggaaacgat gatatcgtat accctctggt
      601 gggtgtagga gcggactttc gacgtccaaa catctcctgc gatattcagc gtcttgcttt
      661 tcttggcaaa aacaagagga cacccgaagg tctgcgccgt tatgcacgca ataccgatac
      721 cggatgcctc gatcgtaaga atcttattga caccgcagtt atggtacagg cgctcaaact
      781 ctttggcaag ttcggagaag aacacgacgt ccatctgatg gttcagaaaa ctgtccacct
      841 tcaaaacgcc gccgtcacgc actacaccat cgcggcggat cctatcttcg aggagctgca
      901 tccgtatcac ccccacggct taacttgatt tcatcttatc gaatttgttc cggttttgca
      961 atgcatatat cttgtgaatt tcggagaaat atatttcgat attttgtgca taacagaaaa
     1021 caccgtcgaa tggggctcac ccatccgacg gtattttgtc atttgcttat ttctcttcgg
     1081 cgtaggtgtc gaagtaaccc tgaatgaaca cgaccggcgt gcccttgtcg ccgctgccgc
     1141 tcgtaagatc gcagagcgag ccgatgagat cggtgagctg gcggggcgtg gtgccctgac
     1201 ttgccatgct gcctttgaga tcggcatcct tgtggcggat ggaatcctcc acggcggagc
     1261 gcagctcttc gccgttgaga ccggcaaaat cgttgtcggc gagatacttg atcttcagct
     1321 cgttcggctt gccgacgaga ccggcggtgc aggcagggga gaccacggga tcggcgagct
     1381 cccagatctt gccgacggga tccctgaagg caccgtcgcc gtagatcatg cactcgatgt
     1441 tctttccggt tgccttgcgc atggccgcgg cgagtgcgtc aacaaattcc gctccgttgc
     1501 gcgggaagag cttcaccttg tcctcggagg acttgttgga gccgagcagg ccgtatttct
     1561 cgttatagcc gctgccgtta acgcttgcgg tgagaagatc atccagcgcg aggaccttct
     1621 ccgcacccgc tttggcaaga cggcgctttg tgcggaaccg cgtgtggatg tcgcagcaga
     1681 ggacattttt tacgcgcgtg aggacggctt ccgcctgatt ggcgaagagg atctcggcct
     1741 cgcagccgca ttcctcgatg agttttttat aatagtccac atagtccacg ccggtgaacg
     1801 gatgaagcgc gtagccgaac gccttgcggt attcttcctc agtcagtacg tcgctgtacg
     1861 gattgatacc ggaatcctcc agcagttcct cgtcaaagag atggtttccg acttcgtccg
     1921 aagggtagct gagcatgagc gtgatctttt tcatgccggt ggcgatgccg cgcagcacga
     1981 tggcaaagcg gttccgggag aggatcggga aaatgacgcc gatatggtcg cagccgaatt
     2041 ttgctttcac gtcatcggca atgttctgca ccgtcgcata gttgccggcg gcgcgggcca
     2101 caacggactc cgtcacggcg acgatgtcgc ggtcgtggaa ggtgatatgc tccgtctcgg
     2161 aggcggccat gacgctgtcg cgcaccagcg tgacaagatc gtcgccctcc cggacgatgg
     2221 gggtacggat gccgcgaaca acagcaccaa cagttctcat aacgaaataa cctccgaagt
     2281 attggatttt cgccgatatt atgctacaat accgtttgtc tgtaaagatt taattttcga
     2341 tttttgcaat ttttctattt gcgtcagtca aaaaaagcac attttgcagg agttgccatg
     2401 agaaatctca aaaaattttt gatcgtctgc acctttttcg tcgctttggc tcttctggcc
     2461 ggctgcgctt ccgcacccgc gccggagatc gccgccacag aagcaccgac cgcgacgccc
     2521 tctccgacgg ccacgcccgc gccgatctca ttcgaggctg cggctgaggc aaatcgcacg
     2581 gaactgcgca cgtttttgga ttacggcagt gtcttctccg ccgtgcagga gagcaaagcc
     2641 gaggaagacg gcgcggacgt tccggcgcat tcctccgcgc tgcgcgccgc agcatcgccg
     2701 gagaatatta cggaaagcga catcctccgt gttgacggtg agtatatcta tacgcttacg
     2761 gacaaaaatc tcacgatctt tcatctgagc ggtgtggcgg gggagcttat ttccactacg
     2821 ccggtcggta ccgcctggag cagctccgga gacggttccg gcagctttgc cggaagcgaa
     2881 cgtacgccgc tcgcgctgtt ccttcgcggc tcacggctcg ccgttcttct ggacgtgtac
     2941 ggctacgaga gcttcgcggg cgagatgcgc tattcggaat atgtcggcgt cgatttctac
     3001 gacgtttccg atccgcacac gcctgtgctc ctcgcgagcg ccggtcaaag cggcgtatac
     3061 tccgatgcgt ggatgagcgg cggcgctctc tgcgttgcga cggacttctc cgtgctggac
     3121 acctccgacg ccgcagatac ggacaagttc attccgcgcg tacataccgc cgcggacagc
     3181 cgccagcttg aggccgaaag cctttacgcg ctgccgaacg gcggcgaggg ctgcaccgtg
     3241 ctcggcggct actctctcga cgaggcggcg cagaaaaatg ccgtcgccgt ctacggcatc
     3301 gaggcgaaaa acgtatacgc tgtacccggc agtcttttcc tgaccgacac acgctccgta
     3361 tccgccgaat cccgccgcat gagcgacagt gtgggtgatt atacggaata cgttgagctt
     3421 ctctgcaccg atcttttccg ctttgacttt gatgcttccg gcatctgtcc ggcggcctcc
     3481 ggcgtcgtga gcggacttct tccggaaagg agcgccattg cgccgtttgg ttccgggtat
     3541 gtctgtcttt cggaagtctc gggctcttat acaaatatgt atgttggcaa gggcggtccc
     3601 gctccggccg cggaacagac cggcagcgcg ctttatacgc tcgacgccgc tcttaccgtc
     3661 acggcaaagc tctccgcgct tccgaacggc gacagtatac tttgggcggg tttcacgccg
     3721 gaaaccgtgc tgctctccgg cgagaacgga tcctgcaccg ccgatctctc tgttcccggc
     3781 acgatcaccg ccttgcccgg cggcgatgcg atcctggcgg atgcgctgct tccgtggaaa
     3841 aacggcggct atgccgcctt ccggcatgag agcgccggac aaatggcgct tgcgctctat
     3901 gattcatctc tgaagaaaac cgcggagcgc acgttcggca gcgactacag cagcacactc
     3961 gaaagtctgc aaagctatat cgtcctgccg gagaaaaacc ttctcggctt tgccgccgac
     4021 gacagctatt gcctgtatgc cgaaaacaac ggagagatcg aatactgcct gagcgtattc
     4081 ctgaccgact gggcttggaa cgcccgcgct tttgaacaga acggctatct ctgcatcgcg
     4141 gatcgccgcg aggcgttcgt ctatctgccg gacacgctcg aaagcgccgc atcttttctg
     4201 ttctgaccgc ccatcttcgg caatcttttc ataatacgcc cgttaaaatg gcgctgtgga
     4261 caaccacagc gccatatctt tttcaggggg atgagtgtca tgcaattgga gagcgcctat
     4321 accgacggtt gcctttgcat ccgatatacc ggagatctcg atcatcacgc ggcgcgcgcg
     4381 gcgtttcgcg aaacggaagc gtttatcgac cgttttctgc cgcggcggtg cattcttgat
     4441 ttctccggcg tttcgtttat ggacagttcc ggcattgccg ttgtgctccg cgtgaaaaaa
     4501 cggatggagg aaacggacgg ggatgtcttt ctgcttgatc cggcgccgca atgcgcaaga
     4561 gttcttcgca cgtccgggat cgaacgcttt gtcgcaatca aatttacgga gggaaaggcc
     4621 gcaagatgaa atacgaaaac tacataaagc tggaattccc cagcaaaagc tgcaacgagg
     4681 cactggcgcg gtcggtagcg ggcgtgttcg cctcacagat ggacccgacg atggaggagc
     4741 tcggcgatat caaaacagcg gtcagcgaag ccgtgacgaa ctgtatcgtc cacgcttacc
     4801 cggactgcat cggcaagatc ctcatgcgct gccggatcct tgaggataac cggctggaga
     4861 tcgtcatccg agactggggc cgcggcatcg aagacatcgg caaggcgaga aagccgttgt
     4921 atacgaccgg tggcgaggaa cgcagcggta tgggctttac gatcatggag agctttatgg
     4981 ataagctcac cgtccgctcc acgccgggcg aggggacaag cgtcatcatg ctccgcacgc
     5041 tcccgcgccg cgtgcgcgac cgctgatgga aagccgcgcg cagcttctct cccgcgcgca
     5101 ctccggcgac cgggatgccg tgcagcagat gatcgaggaa aacaccggcc tcatctggag
     5161 cgtcgcccgc cggtattttg gccgcggggt cgatgcggac gatctctatc agcttggctg
     5221 tctcggtttt ctcaaggcca ttgagggctt tgacgagcgg ttcggcacgc agttttccac
     5281 ctatgccgtg ccgaagattg ccggggagat ccgtcgcttt ctgcgcgacg acggcgcggt
     5341 aaaggtcagc cgcgggctca aagagcgcgc gcagcagatc catgcggcac ggcgccgtct
     5401 ggagcagacg ctcggcaggg agccggcgct ttccgagatc gccgcggaat gcggcgtgag
     5461 cgtggaagag atcgccgagg cggagaccgc ggccgctccg gcggagagcc tgcagcgcga
     5521 gagcggagac gagggcttta cgcttgagca cgtgctcggt gactatgggc aggaggatcg
     5581 cgtgctggag agcgtcgcgc tccgtacggc nnnnnnnnnn ccgtacggcg atcgagcgtc
     5641 tgccggaaaa ggagcagcag gtgatcaatc tccggttttt ccacggcctg acgcaaagcg
     5701 actgcgcgcg gatcctctcc gtatcgcagg tacaggtatc gcgcctcgaa cgccgggcaa
     5761 tggaaaatct gcgcgccgtt atgctctgag ctgtgcggca agaatgcatg ccgccggaag
     5821 tgcgagaccc cagcggcgcg tgagcgctgc cgcagcaaga catattgtcc cggtacacgc
     5881 cgaaacgatc cgaacggcct tgcggtcgcc gcagagcgca taaaggatcc gtccaccgtc
     5941 gagcggttgc gccggaagca gattgcacac cgaaaaatac agcgatatgt cccggcattg
     6001 cgcccaccac tcgccgggaa agcgtgagca aagcgccgcc cacagcaaac cgcacaccgg
     6061 ccctgccgcc gcgcacactg cctccggaag cggcgacgaa aacggtgtcg tatcgaggca
     6121 cagcccgctc agactcatgc ggagagaaag gatccggcca ccgtacatcc atgcggaaat
     6181 acaatggccg atctcgtgaa agaacacagc gggcagaacc gcggagaaaa tgccggaatc
     6241 gtccagataa aagagcaggg ggaatacaac ggcagctccg gcggtgatct ctacccggca
     6301 gcggctcaaa tcgccgccat gtagaacgcc ggattcagat aaacgccgtc gtgcgtaagt
     6361 tcaaaatgga gattcgggcc ggtaaccttg cctgtcgcgc cgacaagcgc gatcttttcc
     6421 ccggcattga ccttctgccc ttcttcaaca tagatctggc cgcaatgggc gtacatcgtc
     6481 gcgtagccat cctcgtgctc tacgcggaga aactttccgc tcgtgccgtt ctcgccgact
     6541 tccgtcaccg ttccggacgc aaagcagacg atatcgtcgc cggagtttgc cgcgagatcc
     6601 atgccgtagt ggaactttgt ctcgttgtcg atcgggtgca gccggtagcc gaaatcggag
     6661 gatactctgg cggaaagcgg ctgaacgaag gagaacttcg gcatgggcgc gtcgtaggag
     6721 acattggccg gaacgccgag atcgacgaac ggctgctgct cttccataaa cgccgccatc
     6781 gcctcctcta cagcggggga cagctcttcc gtgccggctg gttccgtaag tccgagaaac
     6841 tccgcggcgt tgatcgtgac cgtgaccggc attgccgcat cgtccgccgc ctccgtcaca
     6901 cctgcgacgg tttcctccgt cctgtagtct gctctgtgga aagattcttc gactgtacgg
     6961 atcgtttcgg atgtgcgggc agggaagaga agcttcgcgg cggccgccgc aacaaacagt
     7021 cctgctgcca catagatggt tgcgcgtttt gcacgtttca ttccaaatca cactcctgcc
     7081 aaagcgtatt cggacgagtc acaagctatg aatccttttt tgtcgcttga cttttcaggc
     7141 atattttggt agagtacatc agtaaacaaa atacgcggat atagttcatc ggtagaacga
     7201 cggcttccca agccgtagag gtgggttcga ttcccattat ccgctccatg ggaaaacccc
     7261 ttgaaatgct cgaaaaacga gcaatttcaa ggggttttcg gcgttttgcc gtgctgttat
     7321 tctacgctgg atggtatggc agatgtacgt tttgaagcgt tttttgacac ttttttacta
     7381 acggatttac taacggaggc ggagtagaac ttgcgcatct tcttggcaga gcgtgagagg
     7441 tctttttctg aaagacggag ataatgatcg tgaacggttt tccagttgct ccacccgccg
     7501 acgcgcatgg tctcctcttc cggccaaccg agatggtagg cgagggaagc aaagctgctg
     7561 cggagccctt ggaagccgac ctctgggaga tcgtgtgcag cgcagatgcg gttgatccga
     7621 cgacgggtgt tatcgttgca tggacacata cctccggtat gatcttggag cagctccagc
     7681 aggcggggaa tcacgatggg gacaacgcgc tgcgagaggg cggacttgtt ctcctcgcgg
     7741 tggatccatg agccatcgtc ggcgtagacc gtggagccgg aaacacggat cgtctcggac
     7801 ttgcggtcta tgtcctccat gcgcaaggcg aagatctccg agcggcgaag agagtgcagc
     7861 gcaaggagcg cgggaagctc gacgggagtg tcgcggatcg cgtccacgaa ggttagtatt
     7921 tgcgcatagt ccagccacgg agtatcggcc tttgggacga cgccgagatc cacggggtca
     7981 aacggtatct tctgctcctt gtaggcggcg gcaacaaagc cgagctcatt cttcagggtt
     8041 ttctttgcga cgcgctgtgc ttctgacgca acggcgcgcg gccagtttat agagcggata
     8101 tccttatcca tgcagctggg aaaagcgctg ttgcggatgc attgatagct tcgcctggta
     8161 gacggagaaa gcatgggacg gtcttccaga tacttatcga ggagctgccc gagcgtgagc
     8221 cgggggagcg ccttctccgc ttccagaaac ccggcgcgga tggcgagggc tttggtgcgg
     8281 catgcctcct ccgtgggctc ggtgacgttc acgccctcgc ggcggatctg caccgtccag
     8341 ctgccggagg ggagctgccg gggagcgggg atcttgagct tcttttcctt ttcgcggagc
     8401 tgcttctttc cgcaccagtt gcagtacagc gagttttccg ggatatcgcg cgagcagttg
     8461 cag
//
LOCUS       DAWAAE010000086         8409 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3793_length_8409_cov_3.498085, whole genome shotgun sequence.
ACCESSION   DAWAAE010000086 DAWAAE010000000
VERSION     DAWAAE010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_8409_cov_3.498085"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5910..5919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1723 a         2502 c         2621 g         1553 t
ORIGIN      
        1 aaaagcccgc cgcggaatcc atccgggaca agctgacgga ggagcagtac cgcgtcaccc
       61 aggagagcgg cacggaacgt cccttcagca atgagttctg gaaccagttc gagaagggca
      121 tctatgtgga catcgtcacc ggcgagccgc tcttttcctc cacggacaag tttgagagcg
      181 gctgcggctg gcccgccttt accaagccca tcgaggagcc tgccgtggtg gagctggagg
      241 atctgagcca cggcatgcgc cgcacggagg ttcgcagccg tgccggggat tcacaccttg
      301 gccacgtgtt caccggcgac ccggaatccc ccaacggcgt gcgctactgc atcaacagcg
      361 cctctctccg cttcgtgccc tacgcgaaga tggaggccga ggggtacgga tacctgctgg
      421 atctgtttga gaaatgattt caggtgcata cacatcaagc gaaaaactca gacggctgat
      481 cttcaaaaga acagccgtct gagcctgccg aataacccgg ctgtgcatta agcggcagcc
      541 gggtttctcg acaggctgcg tcaagaccac cggcacagcc ggtggtcttg actgttaaat
      601 gcgggaattc cggcggtttt tacaattttt catattgcat gcgtttcaga cgctgtgata
      661 tactgacaat ggaggaaaac aaaatcggga acagaaaacg gggaggaaaa acgaaatgaa
      721 gttcaaaaag ccgctctctc tgctgctcgc cgcagtgctg ctctgcgcgt tcgccgtgcc
      781 gctgctggaa acccctgcgc tcgctgccga taaagaaata cacgaggtcg ctaccgctgg
      841 agtatacaaa tggatcacga tcacagccgg ggagggcggt gcggtcacgg aggtcgtgca
      901 gactggcggt gtgctgccgc ccggtatgtc gctgggcaag aatgaggatg tgtccagcct
      961 tttcggcacg ccgacaactc ccggcactta catattgacc ggcgagacac actatgcaga
     1021 tgacgcagcc tcttgggtgc tccgttggaa ggtgcggctg tttgtcgatt tcgcagctac
     1081 tccgacgcca acgccgacaa cggcgccgac gacggctccc acaacggctc cgacgacggc
     1141 cccaacgacc ggcacaacga ccgcgccgac gaatactccg gcgccgactg ccggagcgcc
     1201 gatcattaca aagcagccga cgaaggagac cgtggaggtc ggcggcagcg caacgtttgt
     1261 ggcaaacgcc accggctggg cgtggtgcgc gtggcgcttt ctgagtcccg acggcaagac
     1321 cgaggtcatt ttcgacgtga ccgccgataa gttccccggt cttacgatca ccggcggtaa
     1381 cagcacgacg atgtatctta cgaatattcc catggagctg aacggctgga aggcggtctg
     1441 cctcttcagc agcgcaacga atctctggac ctataccgac ggcacggcgg tcatcaccgt
     1501 gaatgccgcc gcaacgccca cgccgtctcc gaccccggag ccgacggcaa cgccggagcc
     1561 gacagcaact cccacgccga cggaagcccc cacgcccaca ccgaccgccg ttccggcggc
     1621 cgtcgttgaa acgccgactc cgtcgcccga gccgaccccg gagccgaaaa agggcggcct
     1681 gctgtccggc ggcaaagcac tcccgatcgt ccttgccgtg gttgccgtgc tgctcctcgg
     1741 cggaggcatc acggcgggag tactgcttgt gcggaaaaag aaacagcgcg aggaatacga
     1801 acgtcaggag cgccggagac agcgcgcttc tgcggcggct gccggaacgg cggcctcccg
     1861 acgctccgcg cccgcggctg aatgggtctg cccgatctgc ggtactgtga acaaaggaaa
     1921 gttctgcagt gagtgcggcg ggggagagcc caagaatcac gtctgagtct cgcacggcag
     1981 accgccgtgc ggacggcttc ggccgaaagc cttgcggcgc gcagaagaga ccggaaaagt
     2041 caaacaatta attcaagctg ccgaaagggc ttgctgcctt gaagaacaga cggcaaaccc
     2101 tttcgcgctg cccggattct gcggctgctg ccgtgacccg tttccggttt ggcgagccgt
     2161 tcccgccatg cacggcagca aaaatataat aatttcagac aaatgtcccg catagaaaaa
     2221 gatagggaga ggatgccgcc ttgaagaaga aagttcttac tctatttgct gtgctgctct
     2281 gcgcgctcgt cgtgctgaac gtcacggctc tcgccgattt cggcgatttt tccggcagct
     2341 cggactatga tttcagctac gattacagcg ataacgacag cagctggtca tcctcgagca
     2401 gtagctccgg aagcagttac tacgatgatg acggatatgt atccggcgac gccaacccta
     2461 cgcggatcct tataatggcg ctgatcgtag tggtggcgat cgtgtattct atgaggacga
     2521 aaatgaaaaa caacagcacg tctaagaaag cgaccgccca gcgcgccgag cgtgccgaga
     2581 gccgcgccaa ccgcgccgcg ctcacgccca tcggcgaata tacgaagatc gatccgaact
     2641 ttaacgcggc ggcgctgtgc gaaaaggcgt cgaatctcta tgtgcagatg cagaacggct
     2701 ggacggcgaa gaacatcgag tcgctgcggc cgtacttcac cgacgcgctc ttcacgcaga
     2761 tggagcgctc gctgcagggg tatgcgcagc gcggcgagac gaacgtcgtc gagcgcatcg
     2821 cggtgctgga tgtgacgccg ctcggcttcc accagaccgg cggcgaggac catatccttc
     2881 tccggctgcg cacgcgcatc acggattaca ccgtgaacga cggcacgcag cagatcgtcc
     2941 gcggcagccg cgatcaggaa aagttcatga cctacgaatg ggatctgctg cgcccgaccg
     3001 gcacgcagac gaacgccgag agcggcgaga caaagcgcat cacctgcccc ggctgcggcg
     3061 cgccgctcga cgtgaacgcg agcgcgcggt gcccgtactg cggcacggtg atccagcagc
     3121 aggcacagga ctgggtcatc agcgccattc gagggatcaa gcagcagacg ctgtaaaaac
     3181 cggatcaaag gagccgcggc gaacgggatg cgctgtgtga tcgcgccccg ggttcggctg
     3241 gatccggccg ctctgcttgg tcatcaaaaa aactccctgc cgcaagggcg gcctcctgcg
     3301 tatacagtat tggaaaaaag atgagccaga cggatgagtt tcatccgtct ggctctgcgg
     3361 cattctggag ttatgcggga tatttcatca aatgttttcc tgccggatga tctcacggcc
     3421 catgagcacg cccatgacgg aggccatcat cagcccgcgc gtccagccgg acgagtcgcc
     3481 gaggcagtgg agatggcgga tgttcgtgtt gagatcagcg tccatcttca cgcggttgga
     3541 gtagaacttc agctcggggg agtagagcag cgtttcgtac gaggcaaagc ccggcacgac
     3601 ctgatcgacg gccttgatga agttgatgat gttcgtcatg ctgcggtagg gcatcgcggc
     3661 ggtgatgtcg ccggcgacgg cgtcgggcag cgtcggacgg acgttcgact gcgcgagctc
     3721 cttttcccag gtgcgcttgc cgtcgagaat gtcgccgtag cgctgcacga ggatgtggcc
     3781 gttggcgagc atgttcgtga gctcgccgac cttctgcgcg tagctgatgg gctggttgaa
     3841 cggcacagag aagttgtgcg agcagagaat ggagaggttc gtgttcgggc tcttcacttc
     3901 cttgaacgag tggccgttca cgacggcgag accgttgtcg taattctcct gcgaaacaaa
     3961 gccgccggga ttctggcaga acgtgcggac cttgtttttg aacggcgcgg gatagccgat
     4021 gagcttggac tcatagagca cctcgttgac ggtctccatg atctcattgc gcacctcgac
     4081 gcgcacaccg atatccacgg tgccgggctg gtgggcgatg ccgtgctcgg cgcagagcgt
     4141 ttcaagccag tccgcaccgc ggcggcccgt ggcgacgatc gtgcgcccgg cttcgatctc
     4201 gcgctcctct ttgccgttgg agatgacgcc gccgagacag gtctcgtctt tgagacggag
     4261 atcaaggcac tcccagccga agatcagctc cacgccgttc tcggcgagat agcgctcgat
     4321 ggcaaggtaa atgccctggg ccttctcggt acccatgtgg cggatcgggc agtccacaag
     4381 cttgagaccg gcctgaatgg cgcggcggcg gatgtccttc accttgtcgg gatcgctgat
     4441 gccctcaatg tgggtgtccg cgccgaattc cagatagatg gagtcggcgt agtcgatggt
     4501 ctcctgcgcg agcctttcgc cgacgagcgt cggcagatcg ccgccgacct catgggagag
     4561 ggagagcttg ccgtcggaga aggcgcccgc gccggaaaag ccggtcgtga tgtggcagta
     4621 gggcttgcag ttcatgcacc ggcccgtctc ggccttgggg cagtggcgct tctcgacgct
     4681 ctggcctttt tccacgagcg tgatgtggcc cttgtagccc ttgcggatga gctccagggc
     4741 ggtgaatatg ccggcaggac cggcgccgat gatcagaata tccgttttca tgcttcaacc
     4801 ttcacattct ctatgccgtg cttctcaaac tcggcaaagt attcttccat gcgcgcattc
     4861 agctcctccg cgtcgtcctg gctgatctgc tcggcgctca tgtcgcgccg cgcgagctcc
     4921 tcggccagaa tgtcgtagaa aacggcgtcc tcatagtcgg cgatcgcctc gtggatgccg
     4981 ccttcctcgt acgcgcggga gggatacgag tggccgcgcc agcgctgaac gagcgcgcgc
     5041 atcccgtgct ccgggcagag ggaaaagagc ttttcctcga gattgtcata gtcctcaaac
     5101 cggtcatcat cccggcagga attgagaatc cagttcccga tgtacaccat gtccagaagc
     5161 cgccggaatt ccttttccgt cagttcaagc tgcattacga tccactcctc tctgtgaaca
     5221 tacattgaca tcatattata acaataaatc cgggaaattg ctatacaatt tttcacttgt
     5281 catcgaagac gttttcgcat ataatctata aaccaaatgc cggggagacc ggtgtgtcgg
     5341 gagtgaatgt catgcgaatg aaagacgtta tcatatcggt caccggcgtg cagcagggcg
     5401 tgaacggccc cgatgccatg gagctcgtca cggccggaca gtacggccag gacgagaagg
     5461 aaacgctgct cacctggcag gagagcgagc tcaccggcat ggaggggacg aaaacgtcgc
     5521 tttgcgtgca gccgcgcagc gttgtgctct cgcgcgaagg gacgctcaac acccgcatgg
     5581 agtttgagga ggggcgcaag cgctatttcc tgtacgaaac gccgttcggc tcggcgacga
     5641 tggggctgaa cacccggcgc atcgtcaacc gtctcggcgc gcacggcggc gagatcgaga
     5701 tcgattacag cgtggatatg gacgatacga tcgtcggccg caaccggttt ttcatccgcg
     5761 tgcaggagcc gcgcccgagc catatcagcg atgtcacata tcccaattga gtgatcctta
     5821 tgaagcctcc cttgtgcaaa gggaggtggc aaacccgaag ggtttgacgg agggattgcc
     5881 gttttccgaa gactgcaatc ccccagtctn nnnnnnnnnc ccagtctcgc cttcggcgag
     5941 ccagccccct ttacacaagg gggccttttc atttttacag atgcagcacg ttgacgcaga
     6001 acgccgcggc gaggaacccc gcgagatcgg cgcacagcgc cgcggggacg gcgtggcgcg
     6061 ttttgcgcac gcccgccgcg ccgaagtaga cggagagaac gtaaaacgtc gtctccgttg
     6121 agccgagcat gaccgccgcg gtgcgcccgg cgggggagtc tgcgccgcac gcggtgaaaa
     6181 tatccccggc cacggcaagc gcgccgctgc cgctcatcgg gcgcagcagc atgagcggca
     6241 cggtctccgg cggaatgccg agaaaggaga gcgcgggccg cagcagcgcc gtgaatgcgt
     6301 cgatcgcgcc cgaagcgcgc agcgcgccga ccgccggaag cagacacacg agcaccggcg
     6361 cgatgcgccc cacggtgcga agcccttgga gagcgccgtc ggtgagagcg gaaaaaacgt
     6421 ccgttccgcg aaagagcgcg tatcctccaa cgcccgcggc gagcagcgcg gggagaagct
     6481 gaaaaagcat tttcatggcg ctctccggaa aagccgctcg gcgagcagcc ccgcggccac
     6541 gcccgccgca gacgatatcc acaccgcggg cagaatgtcg aacgcgtttt ccgcgccgca
     6601 ggccgagcgc agcgccgcgg cggtcgtcgg gagaagctgg atggacgcgg tgttgagtac
     6661 ggcgagacgg cacagctcgt cggacgccgc ctctccgccg gagagcgcgt gcagccgctg
     6721 cgcggcgcgg atgccggcag gcgtggcggc attgccgagc ccgagcagat ttgcgctcat
     6781 gttgcaggag agctccccgg ccagctccgg atcgctccgg gacgagggaa acagccgtcc
     6841 cagcaccggg cgcagcgcgc gggagagctt ttcggtcagc cccgagcgct ccatgacctc
     6901 gagaagggcg ctgtagaaga tcatcggccc ggcgagcgag agcgtgagct ccacggcgct
     6961 ctccgcgccc tgcaccgccg ccgccccgac cgcgcccgcc gagccggtga gcgcgccgaa
     7021 aacgaccgac acaaaaagaa ggatgaccca gacgtacgac atagccatgg cgatacctcg
     7081 acaggaaaaa attaccaata aaaaacgggg cagctgcatc ttgcaatttc gtatcgggca
     7141 gtatagaatt ttgtcgaaaa gagtatttag gtaaggggtg tgcggatcta tgaaatcaac
     7201 ggggatcgtc cgtcggatcg acgatctggg acgagtggta ctgccgatcg agctgcgccg
     7261 gacgctggat ctggaggtgc gcgatccggt ggagattttt atcgagggcg acgctatcgt
     7321 ccttcggaag tacgacgctg cgtgcctgtt ctgcggcgcg acgcgcgagc ttctctccta
     7381 ccggggaaag cagatctgcc ggagctgtct tcgccggctt cgggaggaaa cagacgaata
     7441 aataaggctg ccgcatacaa agcatatgcg gcagcccttt ttgtatacct atacccgaaa
     7501 accacacgtc tgacgcgcgg tttcgggtat acaaaaaaga ggctgtcccg tgtgggacag
     7561 cctctgaagt atgtatccgg gtatcctcga ttaataaagc gaggcgatca catccacgca
     7621 tttctcgatg ccgagcgagc cggagtcgag gatgatgtgg tagtggttca tgttgcccca
     7681 ttcgagatcg gtgtagaact gataatagag cgcgcggcgc ttgtccttgt cgcgcagacg
     7741 cttttcggtc ggctcctcgg tctcgccgta tttttccacg atgcgcttgg cgcgcttttc
     7801 aaagtcggca tggacgaaca ccttgagaag atcggcgctg ccttcgagaa tggcgtcggc
     7861 gcagcggccg acgatcacgc agttttcctt ctggccgagc tcaagaatga tgcggcgctg
     7921 gatcgcccag agatagtcct ggttggacgg cacgccgaac gagcgcaccg aggagaacgc
     7981 cgccgccgtc cagctgctgt gggaggcgta ttcgctctcg ttggtgatgt attccttgga
     8041 aagcccgctc tcttcggcca gccggtcgat cagctcatgg tcatagcagg ggatgccgag
     8101 ccgatcggcg acttccttgc cgatggtgcg tccgccgctg ccaaactcgc gtccgatggt
     8161 gatgatgcgt ttcttttcca taaaaacagc ctccttataa ccggtaggtg gtgaatttct
     8221 gtattcgtat tatagcacgt ttctttcccg gggaaaagca aaaattactc ttccgcgtcg
     8281 gtttcttttt cgcgcacgag cgcggcgtgg taaagatcgt ttcggctctg ccccgtctcg
     8341 gcggcggcgc gcttggccgc gtccttgagc ttcatgcccc cggcgcggta ttcctccacg
     8401 agcgtgagc
//
LOCUS       DAWAAE010000087         8340 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3831_length_8340_cov_3.222209, whole genome shotgun sequence.
ACCESSION   DAWAAE010000087 DAWAAE010000000
VERSION     DAWAAE010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3831_length_8340_cov_3.222209"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4141..4150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7606..7615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1699 a         2495 c         2389 g         1737 t
ORIGIN      
        1 ggtgaagact gccgcgggct gccggctcat taccggcaac gaaatgggcg tgctgctgct
       61 tgactttgtc tgccggatgc gccgggcgga cggcacgatg ccggaacgcc ccgtggcggt
      121 aaaaaccatc gtcacaacgc ccatggcgga gaaggtcgcc gcgcattacg gcgtggagct
      181 gcggaacgtt ctgaccgggt tcaaatacat cggcgaacag atcgggcttc tcgaaaaggc
      241 cggggaagcc ggccggtaca ttttcggctt tgaggagagc tacggctatc tgagcggcag
      301 ctttgtgcgt gacaaggacg cggtgaacgc ctcgcttctc atctgcgaga tgttcgtctg
      361 gtataaatcg cagggcaaaa cgctcgcgga cgcgctggaa gcgctgtacg cgcaatacgg
      421 cttttttgag gcgaagctcc tatcctttac gtttgatggc tcggcaggct tcgcgcatat
      481 gacagagctg ctcgactctc tccgtctctc gcctccggca gagctggcgg gcgaagccgt
      541 gcggcaggtc atcgactacg accgggacga aacagggctt ccgcgctcca atgtgctgcg
      601 ctttattctc gacggcggcg aagttgttgt gcgtccctcc ggcacggagc cgaagctcaa
      661 gatctatctc accgccgccc gggaaagccg ggagaagagc cgcgccatgc ttgccttgct
      721 cgaaaagcgg cttaccgatt gggcaaaata aaaggagtgt ctatggcaac atccaaagag
      781 tatatagaat ttgtctgcga acagcttgac gggatcgaga acgtcaccta taggaaaatg
      841 ttcggcgagt acatggtgta tgtcaacgcc aagccccttc tgatcgtgtg cgacaatacc
      901 gtcatggtga aaaaagcgcc ggagctcgct tctcttatgg aaggcgcgcc cgacggcctt
      961 ccctatgagg gcgccaaggt gcattatatt ctcgacatcg aaaaccggga actcgtccgc
     1021 agcgtgattg cagttctgga gccgatcacg ccgcttccga aaaagcgtgc gaaaaagaaa
     1081 gacgcataat ttcaaaaaat tgcctcgcgg ctgtccggcc gcggggcgat tttcgtttaa
     1141 caaggatttt acaaaaaaac cgtagcgcat ttaacattcg ttctgctacg ctgattttgt
     1201 aaattaaata tcaggaaaag gataatttat ggacattttt gacacgctta cgatgctcgg
     1261 cggcctggcg ctgttcctct tcggcatgag tctgatgggg caggcgctcg aacggcgcgc
     1321 cggcggaaag ctccgctccc tgctcgacag aatgaccgga aagcccatcg ccggttttct
     1381 caccggtctc ggcgtgaccg ccgtgatcca aagctcttcc gccacgaccg ttatggtcgt
     1441 cggctttgta aactccgggc tgatgacgct gcgccagtcg atcagcgtta ttatgggcgc
     1501 aaacgtcggc acgaccgtga cggcgtggct tttgagtctc ggcgacattg acggcacgag
     1561 cgtgctcgtc cgcctcctca agccgacctc ctttaccccg atcctcgccg tgatcggcat
     1621 cgtcctctat cttttctgca aaagcagccg caaaaaggat tccggcgtca tcctgctcgg
     1681 cttcgccacg ctgatgttcg gcatggagac gatgagcggc gcggtatcgg ggctgcgcaa
     1741 tgacccggtg ttcgttaatc tcttccttgc gttcaaaaac ccgctgctcg gcgtggtggc
     1801 cggcgcggtg ctcacgggca tcatccagtc gagctcggct tcggtcggca ttttgcaggc
     1861 gctctccgcc accggcgcgg tgagctacgc cgcagcgatc cccatcatca tgggccagaa
     1921 catcggcacg accgtgacgg cgcttctctc ctccatcggc acgaacaaaa acgcgcgccg
     1981 cgccgccgtg gtgcatctga tgttcaacgt catcggcgtt gtcgtcctgc tttcggtgtt
     2041 caccgtagtg cgcgcagcgc tcgccccggc gctgctcgac gaggccgcga cccgctccgg
     2101 catcgccatt gcccattcgg ccttcaacct tctctgcacc gccatgctcc tgcccgccgg
     2161 aggtctgctt gaaaagctcg ccatccggat cgtaccggac agcggcgaga aggaacagcg
     2221 cgtggagctg gacgagcgcc tgctcgcaac accggcgctg gcgctctccc ggagccgcgc
     2281 cgttgccgca gacatggccg agcacgccgt gcgcgcactg aaggattccc tcaccgcgat
     2341 cgacagctac tcccccgctc tcgccgagag gatccggcag gacgaggagc tgtgcgacca
     2401 ttacgaggac attctcagca cctatctcgt aaagctcagc gccgagcaga tgggcaccgc
     2461 ggaaagcgaa gaggccgccg ctcttttgaa atccatcggc gattttgagc gtatctccga
     2521 ccacgccgtc aacacgctcg aatccgccga ggagctgcgc gacaagaatc tcacattctc
     2581 tccgagcgcc gtcgccgaat tttcggtgct ctccggggcg gtcagcgaaa ttctcgatct
     2641 ttcccttgcg tgcttcgaga agaacgacag cgccatcgcc gccgaggtcg agccgctcga
     2701 gcaggtcatc gatcagctca aggaatcgat gcgcacgcgc cacatccggc gtctgcagca
     2761 gggagggtgc agcattgagc tcggcttcat tctctccgat cttctcaccg gtctcgagcg
     2821 cacgtccgac cactgctcca acattgccgg gtgcgtcatc gatacggcgc agcacaatct
     2881 caaccttcac gagtcgctgc gcgccacgcg tctcgagagc gccgacttta tccgcgaatt
     2941 taaccgctac gcccgcaaat acgcgcttcc cgcgtccgcg gcggccacag actgactttg
     3001 tactctctca ctccggcagc gtcgttttgt ccttgtgacg gcggcgctgc ttcctttttt
     3061 gcggatattc gacgggtttc ccttgcattt attcccacct taatggtata cttataattg
     3121 atatgttttc caaaggaagt ggttcccatc gctatcacaa gtgccagcct gcccgccaaa
     3181 cggcggatcc tcaccgtatg cgtaaagctg tttctggaaa agggctacaa gagaacgacc
     3241 gtcgccgaga tcgtgcagaa ggcggatgtt tccaacagca cgttccagaa cattttccgc
     3301 gccaaggacg gcgtgctcac cgagctcgtc gagttcatgt tcgacaacca gttcggcatg
     3361 gcccggcgca ttaccgacgc ggatcttccg ccggtattcg tctatgccgt ggagaccgcc
     3421 atccagctca cgctcacgga gctcaacgag aacctgcggg agatctacat cgaagcctac
     3481 acgcagaacg aagcctcgga gtacatcttc cgccagacgg cgaaggaact gtacgccatt
     3541 ttcggctcct accatcccga aatgaccgag cgcgattttt atattatgga gctcggctcc
     3601 gcgggcatca tgcgcgggta catggcgcat ccctgctcgg aggacttccc gcttgaggaa
     3661 aagctcgacg ccttcttccg gctgagtctg ggcggctacg gcgtcccggc ggaggagatc
     3721 gagcgtgtcg tgcagaagat ccgctcgctc gacatccgcg ccattgcgca gggcgtcatg
     3781 cagcagctct tccacgcgct ggaaatgcgc tacgccttta ctctcggcac acccgagacc
     3841 ggcaattaag gttcatagga tactataatg ccgccggcat atcgcgatct gcgatacgcc
     3901 ggcggtttgt taccagtgga tattcagctc ctgcttggcc gattcgagcc agagcgccga
     3961 ggagcgtccg tccgcgtcga gactgactcc aacggagcat ccgccgagcc acgttccgtc
     4021 ggagcgcttt atatactccg cgtagccgta atttgccgtg tcatcggaaa caagaccaac
     4081 gagccagacg tgcgtattgc cgtccgtgtc cgtgtagtca tagcggaacg ccttcgtcgg
     4141 nnnnnnnnnn acgatgaaga tcatcctgcc gccgcgctgc acgaccggcg gcgtcagatt
     4201 ttccgtgtgg agacgcacct cgttgtagct ttcgccgttt tcgtcaatgc cggtcgtgac
     4261 ctccatatta ttcccccagc cgaaaacatg gccgaggatc agtccgccgc gggcatacgc
     4321 tgtcacggca aggccgagcg ccagcaccgt gcaggcgcag acgagcacga gccgccgtcc
     4381 cgggcggaag gttttccgct ccgccccttt ttccgccgtc agcgaaacgg tatccggcgc
     4441 gtgcagcgcg ccgaacgcct gtttataaag ttctccgtta ttcatccgtc caaacctcct
     4501 gtaacgcttc tttcagcagt ctgcgccccc gggaaaggcg cacctttacg tttgcttccg
     4561 taatgcgcag tatcccggcg atctcccgca ccgagtaatc ctcatagtaa aagagatgga
     4621 tcacgatccg gtacttttcc ggcagctcca tgaccttttc aaacaggtcg cccgcctccg
     4681 gcgacggaaa ggcgagcgtt tccgcataat cctccagcgg tacgccccta cgccgccaga
     4741 aggacatcgt cacatttttc gccttgttga tcgccacacg gagcagccat gcgcggatat
     4801 gctgctcgtc ggaaaactgc gtgtttgtcg tgtggtagcg gataaacgta tcctgaacaa
     4861 cgtcctccgc atccgcagcg ttcgcgcaga cattgaacgc cgcggaaaag atactgtcct
     4921 tatatttttc cacgaccacg cttgtctctg ttttcatgca tgattcctct ttctgaaagg
     4981 cccttcatca ataaaacaat cgaagcatgc caaaggttac aaacggatta tacagcaaat
     5041 gtcccgaaga ggattcttcg ggacatttag tttacataat tattcttcag cctttgagtc
     5101 cgccggcctg caatccggag acgaagtatt tctggcagag gatgaacacg atgagcaccg
     5161 gcacgaggct cgccacggac atggcgaaca catagcccca ctgcgtgaac tggtgctggc
     5221 cgaaaaagcc ggagatggcg atgggcagcg tgcgcttggc gttatcgttg atgacggtca
     5281 tggcccaggt gaattcctcc cacgcggtga ggaagttaaa tatgctcacc gacgcgacag
     5341 cagggcttgc aagcggcaat atgatgcgga agaacaccgt gaatacgctc ccgccgtcgg
     5401 cgtacgccgc ctcgtcaaac tcgcgcggga tgctctcgat gtagcttttg atcataaacg
     5461 tggaaaacgg gatgacccac gcgacataga acgggataag ccccgtgagg ctgttcgtga
     5521 tgccgaaaaa cgacgcgagc tgaaactgcg ggatgatgag cgtcgtgccg gggatgatca
     5581 tcatgccgat gacgagcgcg aaaaggagct ttttccccgg aaactcaaac cgcgcgagac
     5641 agtacgcgag cgccgaggcg atgatcgccg caaggacgat cgtaatgact gccacgacga
     5701 cgctgttgat gaagttgaga tagaatttct cctgcgcgaa gatgtaccgg tagttttcca
     5761 gcgtgatctc gctgagcttc gggaaaaagt gcggcggaaa ctcatagagc gcgccgttcg
     5821 gccggagcga cgtggagagc atgtagatca tcggcatgac cgacaccgcg ccgcccagaa
     5881 gaaggagaat gtagcggagc gcgaggctcc agcggttttt tctgcttttc atgcctctcc
     5941 cctccctcag tcgttctggc gcttcatggc gcggaactgc aggaacgcca ggatcatcag
     6001 cgtgacggcc acgatgaagc tcagcgccgc ggcgtagccg aactcgccgg tgctgaacgc
     6061 cttataatac atccacgtga gcaccgtgtc cgtctggtgc atgggcttgc cgtcggtgat
     6121 catcttgatg gacgtgaaca cgttgaagcc gccgatcgtg agcatgacga gcgcgaagag
     6181 gatcgtcggg cggatgctcg ggagcgtgat gtaccagaac tttttccatc cggtgcagcc
     6241 gtccacgtcc gccgcttcgt aaagctccgc cggcacggac tggagcgccg cgagaaacac
     6301 cacaaggttc cagcccacgc ccttccagat gccgagcagc atggcgaccg tgagcccgcc
     6361 ccaacgggta ttcagccacg ggacatagtc cttcgtaagg tgcagcacgt tgcagaggaa
     6421 atagttgagc atgccctcgg tgttgaatat gtaccggaat acaagtgaaa caatgaccca
     6481 cgatgtgatg accgggatat agtagatgac ccggaacgtc acctgaaagc gcgggatgga
     6541 gtggatgagc accgccgcga gaaggccgag cgccatctgc cccggcacgg tgacgagcgt
     6601 atagatgagc gtattttcaa aggcgatgaa aaacgtctca tcgctcacta ccgccttata
     6661 gttctcaagc ccggcaaagc tcttttgaaa taccgacccg agatcccagc gcagaaagct
     6721 cataacgaag agctttacga tcggatagac cgtaaagagc gcgaaaacga tgatccccgg
     6781 cacgagcagc gccgcgaact gggctttatt tctgccggaa cgcagcatca tggccatccc
     6841 tcccctgcgg aatacggtcg ccccgccgcc agacgcggcg gggcatgata aaactgttgc
     6901 ggaatacgaa ggattatgct aaatttaggc aagcaggccg tcgatcgtga ccgccagcgc
     6961 gtcgagcgtt tccttcacgt ccgcgccgtt gacgaggatc ttcgtcatgg cggtcgtgag
     7021 ctcgttgtcg atctcggacc acgaagcgac cgcggggcga gccttcgccg tggtgatggc
     7081 ctcaagaaac ggagcgaagg aagcattctt cacggtttcg ctctcgagcg ccgtcttgtt
     7141 cacggggatc tggccgcact tggccatctc ttcctcggca aacgggctcg tcataaactg
     7201 catgaacttc cacgcggcgt ccttgtggga gccgttgaac attgcgatat cctcgccgcc
     7261 gagcacggag atcgatccgg cttccccggc gggcagctgg cagttgccgt aagccatgtc
     7321 gggataggcg cccgcaagct ccgcggtctt ccacgggcct tcgagaagga agatataccg
     7381 gccggtgccg aagccgtcgg tcatcgggat atcaccgctg ttgtagccgg aaagctcgcc
     7441 gttcttcgcc atatccgcga gcatctgcac ggcagcgatc gtcttctcgc tgttgatgta
     7501 gccgctggcc ttggtattgt cctcgttcgt gatctcgccg ccgttcgacc agatatacgg
     7561 caggacgttc cagccggcga gcgcgggctc gttgagaccc caaacnnnnn nnnnnccggc
     7621 ctcttcgaga gccttttcgt tatagaagag gatcttcgta ttggtgttga gcgccattgc
     7681 gtagtagtgt ccggagacga agccggtgct catggcgctc tccagcaggt cggcggtgac
     7741 ggaatcaaaa tcggccattt cctcgtcgag cgcggtgaga atgcccatct tctggaattc
     7801 gggcacccac gcgatatcca gacgcgccac atccggcagc gtgttggact gcgcgctgat
     7861 gaggatctta tcatggaggt ccgcccactc gtgcgagacg gcgttgacct tgatcccggg
     7921 attctcttcc tcaaatcggg ggatgagaac attcataaga gtttcgttct ccgccgactg
     7981 ggcgctgtag tgatgccaga agttgatcgt aacgggttcg gtttctgcgc tgcctgccgc
     8041 cggttttccg gtggtctccg ccggcggaac ggggctggtg ctcggcgctt cgttcgatcc
     8101 gcacgcagtg aatgccgcta acatcattgc tgcgagaagg agaagcgcca gcattttcat
     8161 ggtagacttc ttcattttcg tcccttcgct ttcttctgat atatttccgc ggagcacggc
     8221 gcaagatcaa cggtgatccc gccgctttcc gaggatacag gttttctgcc gctgatgcgg
     8281 ggcgtccacg tcccctcggc ggcgattttc gttttcaccg gctcgctccc ggcgttgagg
//
LOCUS       DAWAAE010000088         8295 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3846_length_8295_cov_2.878762, whole genome shotgun sequence.
ACCESSION   DAWAAE010000088 DAWAAE010000000
VERSION     DAWAAE010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_8295_cov_2.878762"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2096..2105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1764 a         2272 c         2514 g         1735 t
ORIGIN      
        1 gcgccgatga ggcagccgtc accgatctcc ggcgcttttc cggcaatctc gccgttggtc
       61 ccgttctggc aaatgcggca gtttgccccg ctaacggcat agcgcgagat gaaaacaccg
      121 tgcagcccgt gcgggagcga cggcacgccc cggatcaccg cgtccgggcc aatgtacccg
      181 ccgtgccgcg cggcgcaacg gctcataaga aatgtcagca cgtcccggcg aaagccggac
      241 tttgcgcact gctgccgccg gtagagccgc cagaagcgcg aaagatcgtt cccgtgcgaa
      301 tagtcaatga tcttctcccg gatgctcata cgtacaaccc cagcagccgg tcgatcatct
      361 gcggaaacga caggccgatc cctttcatca tgttggggaa gcggctgtgc gcggtgaggc
      421 cggggatcgt gttcacctcg ttgaacacca actcgccgga aggcgtgagg aagagatcga
      481 cgcgcgcgaa gcccgagcag ccgagcgcgc gatagattgt tttggccgct tcctgaatgc
      541 gcagctccgt tgccgcgtct acgcgcgcgg gcatgtggat gcgggaggtt ttgagcgtgt
      601 atttttcctc aaaatcgaaa aagccgccgg agagctcaat ctcgtcggtg cggccgacgg
      661 tcagcgtgtc cgtgccgagc acggcgcagc cgacctcaaa gcccggcacg gcttcctcga
      721 cgataacgga gctgtcgtac cggaacgctg catcgacggc ggtgccgagc gcatcgcgcg
      781 attccacgcg cgtcacgccg aaggacgagc ccgcgcgcac gggctttacg aacaccggga
      841 aggagagtgt atccaggatc ttctgccgcg tctccgccgt gttcttttcg agcacgaccg
      901 acttcggcac cgcgatcccg gcaagggaga cgagcttgtg cgcgcggtcc ttgtccatgc
      961 agagtgccga ggcgagcgtg ccgcacccga cgagcgggat accggagagc tcaaaaagtc
     1021 cctgcactgt gccgtcctcg ccgttcttcc catgcagcac ggggaaggcg agatcgattc
     1081 gccgcagcat ccacttgcca ttgtggagtt cccaaaaccc cggcgcaccg cggctcggcg
     1141 agaaggcggc ggggcggagg agcatgggat cgtccaccca cgttccggcg gcgatggcgc
     1201 tgtaggtgcc gagatagtga tgccagtttc cgtctttcgt gatgccgatg ggcagaggat
     1261 caaaccgctc tgtgtcaacg gcctccaaca ccgccgcggc ggattggaga gaaacttcgt
     1321 attcgctcga atggccgccg aagagcacgg cgagcgtttt ctttttggtg ttcatggatt
     1381 atttattcct ttcgtatggg cgtgccctcc ggcagagggt aggcgatctc gcattcgttc
     1441 gtgtacaaaa gctgccgctc cattgttgtg atcacggcgt cggtctcctc gcggaatacc
     1501 tcggcgcgct cgtagatttt tccgccgcgg tttatataca tgaccgcgcc gttgtacacg
     1561 cggtattccc gcgccggggg acggtcggac tcgatttgca caaaaaggtc ggtgtcggaa
     1621 aaatcgatgg cgacgcgaaa gagtacgtcc gtatcgttgc gcgcgcggag gtcgcaccag
     1681 ccctcactca cggtcgcatc cgcgccgcgc gggaagcgct cggcttccgc agcggggaac
     1741 tgttctacgg cgtggccgtg ccgctccgtg accgtgaggg gcgtgtgcag aaagcaccag
     1801 tagagaagat cactcagcat gcaaagccct ccgccgtagg ccgggcgaac gctgccattt
     1861 acgagcacga gcccgtcctt atagggagta tggcggtcgg cgcggcgcgc aagacgccag
     1921 aaggaaaacg tctcgctcgg gtggaggact acgccgttca ttgcttcggc ggcaagacgg
     1981 aggttgtgca ccttgttttg ctgatactcc atcggaaaac cgctgcgctc attgcgcatg
     2041 gcgacgttgg cgcggaaaag cgtgtattca agcggcgttt ttgtccgcac ggcggnnnnn
     2101 nnnnnttctc tgccagcgcc gcagcggaag cagaaacgga aagcgctccg tgagccgttt
     2161 tcttgccata ggaaatcatt ctcctttggg gaagcggaga aataaaaaag ctcccgcttc
     2221 cgatacgggt atcgtatcga aaacgggagc gaaatgtttt cccaagcccc gacgggcgtc
     2281 ttatgttttt ctgcggaaat tcttacgatt ttccaacggc cgatttttct atgggcagcg
     2341 taacggtgaa cgtgacggta tcgtcccggc tctcgccggt gagcgagccg ccgtggagct
     2401 gcgtgatctg ccgcgcgatg gcgagaccca gccccgcgcc gccagtggag gagcgcgccg
     2461 tgtccagccg gtagaactgc tcaaaaatgc gcgagagctt atccggcggg atcgtgtcgc
     2521 cgcgattccg gaagcggaca acggcgaaat tgccgacgcg ctccgccgtg acggtgatct
     2581 ccgtgccctg gtagcagtag agcacggcgt tgcgcagaag gttatcaaag acgcgctgca
     2641 atttgttcgc gtcgcaccga atggggaacg tctcgggtgt ggagagcgcg caggtgaggt
     2701 ttttttctcg tagcatcggc tgaaactcaa acgtaagctg ctcgagcagg cgggagaggt
     2761 cggcggcacc gtattgcagc tcgatggcgg agagattgaa gcgcgtgatc tcaaaaaatt
     2821 cgttgatgag atcctcgaga cggttggcct tgtcgagcgc gacggagagg tatttctccc
     2881 gcagcgccgg ggcgatctcg ccctcgtcgt gcagcagcgt cagatacccg atcacggacg
     2941 agagcggcgt tttgagatcg tgcgcaagat acatgatgag atcgtttttt cgctgctcgg
     3001 cttcgcgccg cgcccggaga ttttcctgcg agcgtgaccg cgcaagattg agctcgtttt
     3061 ccacgtccga aagctctttc ggcagctcaa tgggcggtgc ggcggggtcg gagagcgatt
     3121 tggccgcggc gatcacgtcg tccagatagc cgagcggctg gcgcagcgcg cggaccgtga
     3181 accagacgaa gcccgcgagc agcagcagga caatgagcac aggagagagc tgttccacga
     3241 ccttgaggag atagtagagc ggatcgttct cgctccatgg gaagcggtag cagacaaaag
     3301 cggcaaagaa atacccgccg acgaccgcgg caaagagcac cgcgagcgag agcagatagc
     3361 gcgtaaagag gcgcacggca agcggggaga agcggttcgg gcgtttattc gatctcatac
     3421 cctacccccc agacggtctt gatgaatttc gggttccgcg gcggctcgtt catcttttca
     3481 cgcagccggg cgatgtgcgc catgacggtg ttgttgctgt tttgcagata tttctccttc
     3541 cacacggcct cgaacagctc ctcggacggc acgacgcgcc cctgccgcga gcagagatac
     3601 cagaggatcg aaaactcgat cggcgtgagc gcaagctccc ggccaaagag cgtgcatttg
     3661 tggctttcgc gctggatcgt aaggccgcgg atgtcgtatt cgacgctctc ctccggcgcg
     3721 gcggcgctct ggttgtagtg cccggcgcgg cgaagctgcg tctttacgcg cgcgacgacc
     3781 tccagcgggt tgaagggctt ggtgatatag tcgtccgcgc cgatcgtcag gccgagaatt
     3841 ttgtcgctgt cggccacgcg cgcggtcagc atcaggatgg ggaaaaagcg gctttcccgg
     3901 atcttccggc agagcgaaaa gccgtcgatg tccggcagca tcacgtcaag cagggcgagg
     3961 tcgatgtgct ccgattccac gcagcgcagc gcctctgcgc cggtggcgca ggtatggacg
     4021 gtgtagccgt cgtttttgag atagatctcc agaagatcgg cgatctcgcg ctcgtcgtcc
     4081 acgacgagaa tggtttcgtt catagcggtt ctcccaacgg gcggtttggt cttgagccca
     4141 ttaaaccaca gtttccgccc aaacgcaaga aaccgccggg gaggaggaaa atggctccct
     4201 ctatgaggga gctgtcggcg gcagagccgc tgactgaggg agttgactcc ttccgtctcg
     4261 ccttcggcga gccacctccc tcaaagaggg aggcagcaga aaaaaggacg cgcagtcaga
     4321 agctgcgcgt ccgctctatc gttaaggagc ctgtttattt tttcaaaagc cggttgatga
     4381 agccgagcag catgagcata tcgttctcgt ccagctgccg cataccggcg agcgccttct
     4441 ggatcagctc gggatgggat tcctcaccat tgaaaaactg ctgcggcgtg atgccgaaat
     4501 aatcgcagat ggcaagaaac tccttcatcg gcggcagcgc cttgccggag gaaatgttgt
     4561 atacatatcc gcggctgtgc cccagatcat agctcatctg atattccgaa acgcccttgc
     4621 gtagccgaag ctcggtaatg cggttccgga taaattcctc cgtcattggt atcacctcat
     4681 atagataata ccgaccggag acaattcaat agtaaataaa tatttgtcaa aatatattga
     4741 aacaactaaa aatgtatgct actcttggga tgcaatccaa aaaccttgaa agaaatccct
     4801 aaatcgacaa aaaaagaaag gaaagatcac atctatggaa gcggtaattt ttattgcagc
     4861 tatcgtgggg atcaccgtgg cgtacttcgc ggcaaaggag tttgagctta tcgcgaaaga
     4921 aaaggggtat acgagcaaaa agtatttctg gtggacgttc cttgtcggtt tcatcggtat
     4981 cccgatggtc atcgcactgc ctgaccgccg cggcagcggc acgggcgcgg cgtctgccaa
     5041 tttgccggtg aacgacgacg aaattccgga gatttgaggt gcggatatga gcgagagata
     5101 tacgcgtctt tttgcgctgg aggaaaatct ctattccgat ggatcgccgg tcgtcatctc
     5161 tgcgggcgct ctgctcaagg acaaccagac cggagcggtg cttgcgcagc tcaagattag
     5221 gaatatcgga ggtaaaacga tcaaggcagc cgcggttcgc atcacgtgct ttgataccgt
     5281 gggcaaaccg ctcgaaggta ccgccgaaaa ggagtatctc gatctcactg ccggacgtga
     5341 cgaagaattc ggacaaaagt ctcttgtttc gctgccggat acctcggcgc gctccttctc
     5401 cgtcgcggtg acgtgggtcg cgttttcgga caacacagtc tggaacgcct cccaggagga
     5461 gatatccgcg ctgccaaagc aggactctct tagtgaagtg tttggcgatg aagaactcgt
     5521 taaacagtat cggctgaaat acggcgcggg gacaaagagc tttccgcgaa agtataagga
     5581 tctctggttc tgcgcctgcg gcgcattcaa ccatgcagac gaagaggtct gccatgtctg
     5641 cggccaaaag cttgcggccc tcctgccgtt tgatgcggaa gcgctgaaag cggagaaaga
     5701 caagcggctt caaaaggaag tcgacgagcg ccggagagcc gaagagcaag ccaaagtcgc
     5761 tgcggcggag agcgcaaaga aggctaagaa agcacgaaaa actgccgcga tcgtcgctgc
     5821 tgccatctgc ggttgtgcgg tggttgcagt attgctatat aaaattgtta tcccttccta
     5881 taagctgaat caggctgata aacttgcagc ctctgggaat tatgaagcag catttatgct
     5941 tctggatgag ttaaactata agaacagcga agaaaaacgc gaaagaattc tgcaaacaac
     6001 aaagcgcgag gatgtcggtc tgtattataa gcttttattc aatgagacta atgtaaatga
     6061 tacgctttcc tttggtttat atgaacagga cggtaatgct tcaaatggta aggaaaatat
     6121 tgaatggatt ctcctaagaa aagagaatga aaaagttctt cttattagcc gatatgcttt
     6181 ggactgccaa ccgtatacca caaaatggga agaaattaca tgggaagcct gtacgttgcg
     6241 aaaatggctg aataatgaat tttttaatgc cgcattctca gacgaagaaa aatcaatgat
     6301 tataactgcg gctgttgcag cagatatgaa ccccaagaac ggcacaaaag ccggtgcgga
     6361 tacgaaagat aaggttttcc tgctgagcat tgtggaagtg gagaattatt tcgtctccga
     6421 tagcgagcgt gagtgcagtc caacgacata tgcaaaatct aacggcgtat atgttgccaa
     6481 taatggcaac tgctggtggt ggttgcggtc gcctggttat cttcaaagcg atgcggcaaa
     6541 tgttggtagt aacggtggtg ttggatacaa tggcaactat gtcaattatg aaacaaatgc
     6601 cgtccgccct gctatgtgga tcgatttaag caactaaaag cttccttggc agagggagcc
     6661 attacagcaa aaactgccgg gacagagcct gtcccggcgg ttgtgcttat gaaaattatc
     6721 agccgttgac catcttggcg acctcggcgg cgagatcgtc ctggcgcttc tcaatgcctt
     6781 cgcccttctc aaaacggacg tagcccttga cggtgacggg ggtgccgagc tccttggcga
     6841 cggcggcgac gtgctgctcg acgctgacct tgtcgtcctt cacgaacggc atctggagca
     6901 ggcagatctc cttgaaccac ttgcccagac cgccgacaac gatcttctcg atgatggcgt
     6961 cgggcttctt cgcgttcttc ggatcctgct tcgcggtggc gatacggatc tccttttcct
     7021 tctccacgtc ggcggcgggg acgttgctct cgtcgagata ctgcgggctg agcgcgcaga
     7081 tctgcatggc gacgtcgcgg ccgagctcgg tgacggcctc ggcggaggct tcggtctcaa
     7141 gctcgacgag cacggcgacc ttgcccttca tgtggacata aggaacgcac ttgccctcga
     7201 tgcgctcgaa gcggcggacc ttcatgttct cacggacgga gaggaagagc tcgttcttcg
     7261 catcgtccac ggtgccgttg ccggtgtacc aggggcacgc catgagagca tcgacatcgg
     7321 cgggagcttc cttggcgatg accttcgcca cgccggagac gaactcgttg aagagcgggc
     7381 cgccagcgac gaagtcggac tcgcagttga cttcgacgat cacgccgaca ccgtcgatga
     7441 ccgcggcgta cgccatgccc tcggcggcga tgcgggtcgc cttcttggcc tgggaagcca
     7501 gacccttttc acgcagataa acaacggcct tctcgaaatc gccctcgttc tcgacgagcg
     7561 ccttcttgca gtccatcagg ccgcagccgg tggcctggcg cagctcgtta acagcagcag
     7621 cggtaatagc agccattatt cttcctcctt cgcggtctca gcggcctcga cggtctcggc
     7681 gtcctcgccc tgacggcctt cgagcacggc gttggccatg gtctggctga tgaggcggat
     7741 ggcgcggatg gcgtcatcgt tgccggggat aacgtagtca acctcgtcgg gatcgcagtt
     7801 ggtgtcaacg atcgcaacga tggggatgtg cagcttgcgg gcctcggcga tggcgttgtg
     7861 ttccttgcgc gggtcgatga cgaacagagc gccggggagc ttcttcatgt ccttcacgcc
     7921 gccgagatac ttctcgagct tttccatgtc gcccatgagc ttcatgactt ccttcttggg
     7981 aagcatgtcg aaggtgccgt cttcctgcat cttcttgagc tgggccagac gctcgacgcg
     8041 ggtgcgcatg gtcttgaagt tggtgagcat gccgccgagc cagcgggcgt tcacatagta
     8101 catgccgacg cgctcagcct cttccttgac ggcttcctgc gcctgcttct tcgttccgac
     8161 gaagaggatg ctctggccgt tctcggagag agtgcggaca aagtcatagg cttcctcgag
     8221 cttcttgacg gtcttcgcga ggtcgatgat gtagatgccg ttacgctcca tatagatgta
     8281 ttcggccatc ttggg
//
LOCUS       DAWAAE010000089         8274 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3857_length_8274_cov_2.945492, whole genome shotgun sequence.
ACCESSION   DAWAAE010000089 DAWAAE010000000
VERSION     DAWAAE010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_8274_cov_2.945492"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1503..1512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2188..2197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7749..7758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1622 a         2476 c         2474 g         1672 t
ORIGIN      
        1 gccttttcaa acgagagccc aacgaagtgg gtctcgtttg agagcttgtc aaaaaagtat
       61 ttttgacaag ctcagcggtg cgcccattca ggcgcaccgc aaggccctgc aaaagacttg
      121 agggacgctt cccgctttat acgctcaagt cctttttgcc gctgagtttg aaaaacacaa
      181 gaagcgacac gatcgaagtg agagtcagga tcgtggagta ggccgccgca ttgccgaagt
      241 tgctgcgtat gacctcggaa tagatcgcca cggtgagtgt catggtcttg ctggtgtaca
      301 gaatgaccga ggagctcagc tcgctgatga gcgtgatcca gctcatgatc gcgccggaga
      361 gaacgccggg gagcatcatg gggatcgtga cgcgcagaaa cgttttgagc tgcgatgcgc
      421 cgagactgat ggacgcttcc tccacgctgg ggctgatctg cccgatgatg gcggtgctcg
      481 atcggatcgt atacggcatg cgccggatgg agagcgatat aacgatgatg agcaccgtgc
      541 cgccgagcat aaacggaggc ccgttgaagg cgtacaggaa cgaaatgccg agcaccgagc
      601 cggggatgat gtacgggaac atggtgagca catcgagcac cgaggtgagc gcattgcgct
      661 tgcggacgct gagatacgag atcagtattc cgcatacgac tacgatcacg atggcgatca
      721 ggccgaagaa gtagctgttg gtaatggcgc cggtgttgct cttggcaaag atgttgcggt
      781 agttatcgag cgtaaactcg ccggtgaaga gcgagccgtt gatcgtgccg atgaaggacg
      841 tgacaagcac cgtgatctgc ggcagcgccg cgacgagcgc caccagatac accacgatgt
      901 gggaaacgac accgcgccag cccttcggat ctgccgcctc catgggcttg agcgccgaca
      961 tggaataggt gaaccggcgg ccgagcgcct tttgcaggaa gaagaacata agcgcgataa
     1021 cgatgacgat cagacacatc gtggcggcaa agccgtcgtt cgtgccgacc tcgcccataa
     1081 actgcgtata cagcatgacc gggaaggtct tatacccctc gccgatgatc atgggcgtgc
     1141 cgaagtcagc aaacacgcgc atgaatacga gcagcgcgcc cgcgagcagc gacggcatca
     1201 ccagtgggaa gatgatcttc gtcacacgct ggaaggcgtt gcagccaagg ctctccgccg
     1261 cttcgttcag ggagttgtcc agattcttca tcgcgccgga aacgtacatg tagatcagcg
     1321 ggaaggactg aagcgaaaag acgagaacga taccggcaaa gccgtaaatg cccggcagct
     1381 tcacaccgaa gagggcgttt gcgatgcggg tgataaagcc gttccggccc aggagctgga
     1441 tccacgaata cgcgccgatg aacgggggag agagatagga gatcacgata aggatgttga
     1501 tcnnnnnnnn nntcttcacg ctgcgcagca gatacgccat cggcacgccg atgagcgacg
     1561 ctacaagcgt ggacacgacc gtgaccttaa agctgttgac gagcgcgccc cagtaatact
     1621 tcttcgtgaa gaacttcaca aagtagctca gatcgatctt cccgccggta aaaaggctct
     1681 tgatgagcag cataccgatg ggatagatga cgaacagcaa aaagagcgcg agcaaaacga
     1741 gcgtcagcgc cgtccagccg ttgaagcgct tttctccggc gtatctcata aaagctcctc
     1801 ccggatgacc agagagcgct ttccgtcggc ggtaaagaca ttgatgcggc gcggcttcac
     1861 gcgcaggcgc acctccgcgc cgtccggaat gaccgcccat tcctcgtcgc tgtcgcggac
     1921 ggcttcgatc tcctgcccgg tggggagcgt caggaaataa tgcgtggtgg tgccgaggaa
     1981 gacactgctg cgcaccgtgg cgggaatgcc ctcgccttcg ccggtcgaga gcaggaattc
     2041 ctccggccgg accgccgcga gcacgtccat gccgtcggat gcctcgccgc ggagattcgt
     2101 catcgccgcg cggtaatcgc tcccggcaaa gcagagctcc gcgccgtcct cgccgcggga
     2161 gaccgtcgca ttcaggaagt ttgagagnnn nnnnnnngtt tgagaggccg atgaagttgg
     2221 agacaaagcg gttggccggg cgctggtaga cgcgctgcgg cgtgtcgatc tgctggataa
     2281 cgccgccgtc catgacggcg atccggtcgg agaccgcgag cgcctcctcc tgatcgtggg
     2341 tgacgtacac ggtcgtaatg ccgaactgct gctggatgcg cttgatggcg ttgcgcattt
     2401 ccacgcgcag cttcgcgtcc aggttggaca gcggctcgtc catcagcagc acctccggct
     2461 cgatgacgat ggcgcgggcg agcgccacgc gctgctgctg tccgccggag aggtttgccg
     2521 gcatgcggtc cttgagatga tcgatcttga cgaccttgag gatgctgttg accttgcgct
     2581 ctatttcatc cttcggcagc ttgcggttgc gcaggccgaa ggccacgtta tcgaatacat
     2641 tcatgtgcgg gaataccgcg tagttctgaa acaccatgcc gatgttccgt ttgttggtgg
     2701 ggatatcgtt gatgacgcgg tcgttgatct tgaacgtgcc gccttcgatg gagttgaatc
     2761 cgatgatcat gcggagaagc gtcgttttgc cgcagccgga ggggcccagc agcgtgaaaa
     2821 attcgcccgg cttgacttcc agagacaggc cgttgatgat cgtgtcgttg ccgtagcgct
     2881 tgacggcgtt ttcaatggta atggtggctc ccatcgtttc agttcccttt ctctgcgaga
     2941 ttctttgtag aatgtgcggg tctgcggcgc acggccgcag acccgcacgg gatgctttcc
     3001 ttgcgaatta agccacgttt tcgaggcact cgctgtattt ttcggtgatg gcggacttgt
     3061 tggcggcgac ccacacgccg tcataggact ccagacgggt gatctcgctc cacggcgtaa
     3121 ggctgtcgtt gagctggacg ttctggcgga gcggacggac cgtaaggttc tgtccgacat
     3181 agttctggca ggtctcactg agcatgtagt ccacaaactt cttggcgttc tccatgtgcg
     3241 gagcgccctt gatgatggac acgcactgac cggacatcag agcgccctct tccgggaaca
     3301 cgaccttcac ggcaacgccg tcacggacgt aagcggcagc ggggtcttcc caggtcaggc
     3361 cgacgatgta ctcgccctcg gcaacgccct tgtagacctg cgaggagctg gtgagcgcaa
     3421 cgccgtcgag attctcaacg agcttggccg cccaatccca cgcggcgtcg gacatcgggt
     3481 cgccgctgcc catgtcatag agaccggcga tcaggcactg gaacgcggag ctggagttca
     3541 cggggtcgcc cgcggcgatc ttgcccttga gcgcggggtt gagaaggtcg gcaaagccgt
     3601 tgatctcgat atcaccggcg agatcggtgt tcacgatgaa cacggtgggg tcgcagaagg
     3661 cgggggaaga ataaccggtg gtgttcttgt agccttccat catgttggcg tcttcggggg
     3721 agacatactc ttcaaagcag tcggtgtaat ccttcagcat ggcttcgtcc gccgcccagc
     3781 agatatcgcc ctggggattg gcagcttcgg aacgcacacg tttgaggacc tcgccggtgc
     3841 cgccggtgac gacctcgacg tggatgccgg tgtccgcctc aaagttctcg atgacggcgt
     3901 tgttcaggct ctcgcttctg gcggtataga cgatcagggt ctgttcgcct tcggcgggga
     3961 ctgcggtgtc gccgccctcg gcgggagtgc tcgtctggcc gcacgccgca agcgcgagca
     4021 ccagcgttag cgccagcacc agagccatga gcttcttcat tttcatcttc atttcctcct
     4081 tttgttttga gccgttacga ctcattgcag ttttattgta ctataccgaa caaggaagta
     4141 aatcccacaa aacgcaaccc tttcagggaa tttttcaaac taaatgccgg ttttatataa
     4201 aggaaaacgt ttcaatttat tcactttgca caatacgcaa aaaatccccg gcacagacat
     4261 ccaatgcccg tgccggggac tctcaaaggc tttattgtcc gtcaaagtgg acggtgatct
     4321 tggttccggc gtccggctcg ctccagagct caaggcgcgc cttgtgcatc gccgcggcgt
     4381 gcttgacgat ggaaaggccg aggcccgtgc cgccgagctg ccgggaatgg ctcttgtcca
     4441 cgcggtaaaa ccgctcgaac acccggttct ggtgttcata cggaatgccg atgcccgtat
     4501 ccgccacaga caaaaccgtt tcaccgccgc gcttttccgc cgtgacagtc acactgccgc
     4561 cgggacggtt atactttacg gcgttgtcgc agaggttata gacaagctcc tgcagcgccg
     4621 tgcgcacgcc ctgtacgacg gcctccccgc cgcggagcgt gagcgtcacg tcccgccgct
     4681 ccgccgccgg gcggagagag gcgagcgttt cctccgcgag agcgtaaaga tcaacgctct
     4741 ccgtctccgg cgcggcggtt tcctcttcga gacgggagag ctcgatgata tcgtccacga
     4801 gcgccatgag ccgctgggat tccttgtaga tatcaccggc gaactcgccc acccgctccg
     4861 gcggaaccat gccgttggcg agaagctccg caaacccgga gatcgacgtg agcggcgttt
     4921 tgagctcgtg cgacacgttc gcggaaaact cgcgccgcag cgcctcgcgc tgctcgcgct
     4981 cggtcgtgtc gagcgcgatg acgaccgagc cggtgatctg gttttcggaa aagacgggcg
     5041 ttgcgatcac atagtaatac cgctcgtgca tttcaagagt cagctccgtg cggcggccgg
     5101 cgagagcgcg ctcggcggcg cggacgatct cctccgggca gccgccgcgg ttgaggttgt
     5161 ccgcctcgcc gtccggcggc gcgggcagaa gatagctcgc cgatacattg cgggagagaa
     5221 cgttcagccg cttatcgagc aggaggaaac tctcccccat gttctccgtg atcgcggcaa
     5281 agtcgcgccg cgtctgtccg agcgtttccg cctgctggcg gatgagacga ttctgcgcgc
     5341 ggatgcgcgc aagcagcggc acgagctctt catacggcgc ttccgtgccg ggcgtttccg
     5401 ggtcgatctt ccggatgggg tcaacgatgc cgcgcgctac ggcccagccg cccgccgcgg
     5461 cgagacagag cgcgatcggc agcagccaca aaaggtgcaa tgatacctgc gctctcgact
     5521 ctgtcccggc aagctgcggc tgcgcgatgg catacgtcac ggcggagccg agcaggatcg
     5581 cggcgaagag cagaagcccc acacgcagaa agagctttcc cttcatggcg cgctcccctt
     5641 ctccggggta aagcggtagc cgacgccgcg caccgtttcg atgtatgccc ccgccgcgcc
     5701 gagcttggtg cgcagcgtgc gcacatgcac gtccacggtg cggttttccc cgtcaaagcc
     5761 gctgccccat acgctgtcca tgatccggtc gcgcgtgagc gccgcgccgg aatgctccag
     5821 cagcaggcag aggagcgaaa attccttgtt cgtgagcggc acatcctgcc cggaaacgcg
     5881 cacgatgtgc ttgtcccggc agacgtacag ctcgccgacg gtatgctcgc ccgccgacat
     5941 catcccggtg cggcgcagca gcgcccgcac ccgcgccaga agctccatca tgccgaaggg
     6001 cttcgatata tagtcgtccg ccccggcgtc cagtccgacc acgcggtcgt actcggcgtt
     6061 ctttgccgtg agcatcacga ccggaagctc ccgccagagc gccgagccgc gcaatttttt
     6121 caaaaccgaa aggccatctt cttccggcag catgatatcg agcagcacga gctccggcgt
     6181 ctggcgtttc attgccgccc agaatgccga ggggcagcta agccccgcgg cttcgtatcc
     6241 cgcgtgctgc agcgtatagg taacgagctt tcggatgctc tcatcgtctt cgagaaggta
     6301 gatcatctct tccgcctccg ttttgcaatt ccatctttct atagcatatc acgcgcagcg
     6361 ccgcttgtaa aatggattta acgtggattt aaccttgtct tgcttttgga tttaaccttc
     6421 atacggtaca ctgatatgcg gataatctat aacttgcaaa ggaatcgatg gctgtgggta
     6481 tttccaacat tttagcgctg ctcggcggcg tggcgctttt tctcttcggc atggggctca
     6541 tgggcgacag cctgaagctc gtcgccggtc agcggctcga gctcatcctc taccggctct
     6601 ccaatacgcc gctcaagggc gtgctgctcg gcaccgccgt caccgccgtg atccagtcct
     6661 cctcggccac gtccgcgatg gtcgtcggct ttgtgaactc cggcatgatg aagctctcgc
     6721 aggcgatcgg catcgtgctc ggtgcgaaca tcggtacatc cgtaacgggc tggatcctct
     6781 gcctttcgta tatcagcagc agcgacgcgc tctcccttct gagctccgca acgatcaccg
     6841 ccatcgccgc cattgccggc atactgctgc gcatgctcgg gaaaacgtcg cagaagcggc
     6901 acaccggcga tatcctgctc ggctttgccg ttttgatgta cggcatgaac gcgatgagcg
     6961 gcgcggtgtc cccgctgcgc gagagcgagg gctttctccg tctgatgacg atgtttgaaa
     7021 acccgatcct cggcgttctc atcggcacgc ttattaccgc cgttttgcag tccaactccg
     7081 cctccgtcgg tattttgcag gcgctctccg tgaccggaac gatctcgttt gccacggcct
     7141 tcccgatgat catgggcatg ggcatcggcg cggcggtgcc agtgctcgtc tccgccatcg
     7201 gcgcgaatcg cgacgggcgg cgcaccgcgc tcatttatct tttcaccggc attttcggca
     7261 ccgtcatctt ctctgcgttc ttctacggcg tcaacgcctt ttccccgttc ccgttcatgg
     7321 agaccgcgct cggcccggtc ggcatcgcga tggtgaactc ccttttccgc atcggctcga
     7381 tcctgatcct tttcccgttc ctgcgccagc tggaggcgct caccggaaag ctcatccccg
     7441 agaagacctc ggaaaaatac aacgagctca ccgcgggcat cgagcggctg gaagagcgct
     7501 ttcttgtcca ccccgcgctt gccattgagc agtgccgcct gacgatcaac gccatggcga
     7561 tgcgctctca ggataacctt gtcgccgcgc tcgagctgat aaagaatttc tcccagaaag
     7621 gctttcagca ggtcgtggac atggaggacg ccgtggacca gtacgaggac catctcggca
     7681 cgtatctcgt caaattcacc ggccgcgagc tttccaccgc gcagaacgag gaggtatcca
     7741 agttcctgnn nnnnnnnnaa gttcctgcac accatcggcg attttgagcg catcagcgac
     7801 cacgcgatga atctcgccga ggtcgcgcgc gaaatttacg aaaaggacat ccgcttcacc
     7861 gacgccgctc agcgcgagct ggacgtgctc cgcaccgccg tgagcgagat cgtctcgctc
     7921 gttgtcggcg cgttcatcgg gaacgatctg gaaatggcgc tgcgcgtcga gccgctggag
     7981 gatacgatcg acggattgtg cgacgagatg aagctccacc acatcatccg tctgcaggac
     8041 ggctcctgca cgctcgccat gggctttgtt tttaacgacc ttctcacgaa ctacgagcgt
     8101 atcgccgacc actgctcaaa catcgccgtg gcgatgatcg agctggaatc cgacagcttt
     8161 gatacccacg aatatctcaa aagcctccgc gagatgaaaa cgcagaaata catgcagtac
     8221 gcagaggaat acggcaaaaa attcgcgctc tgacggcgta cggccttgtt tttt
//
LOCUS       DAWAAE010000090         8091 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3953_length_8091_cov_2.828771, whole genome shotgun sequence.
ACCESSION   DAWAAE010000090 DAWAAE010000000
VERSION     DAWAAE010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_8091_cov_2.828771"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1689 a         2326 c         2471 g         1605 t
ORIGIN      
        1 gctgcactct ctccgcgccg cgggtgctta aaaagcagca gtccgccgag gacggcacga
       61 tcaagttcct ctgggagctg caggacggaa acgccgtgga aacggtcgtc atgcggtatc
      121 actacgggaa tacggtctgc atctcctcgc aggtcggctg ccggcaggga tgcgcgttct
      181 gcgcttcgac catcggcggc cttgcgcgga atctcacgcc gtcggaaatg cttgacgagg
      241 tgctgttttc cgaaaaggaa tccggtctgc cggtttctca tatcgttctg atgggcattg
      301 gcgaaccgct ggataatttt gataccgtca tgcggttttt gcagctcatc aaccacccgg
      361 agggacgcaa catcagcatg cgtcatatat cgctctccac ctgcgggctg acggagcgct
      421 tcgacgatct ggcggagcgc gatctccaga tcacgctttc cgtatcgctg cacgcgccgg
      481 acgacgaaac acgcagccgc atcatgccgg ccaaccgcgg ccgcggcgtg gacgagcttt
      541 tccgcaaatg caaagcctat tatgacaaaa ccggacggcg catatcgttt gaatatgcga
      601 tgatcgacgg cgtgaacgat tccccggagc aggcggagct gcttgcccgg cgggtaaagc
      661 cgctcgcggc gcatgtcaat ctcattccgc tcaaccatgt ggatgagcgc agctttgagc
      721 catccaaacc ggagaatctc aaacggtttg tgaatatact tacgaagaac ggagtcaaca
      781 cgaccatacg gcggcgtctc ggcagcgatg tggacgcatc ctgcggacag ctgcgccgca
      841 aaatgacgag ggaagcaaaa tgaacgcttt cggcattacc gacaagggaa aagtacgttc
      901 cgccaatcag gacgcctacc gcctgagcct ttcgccggat ggcgaatatg ttcttgcggc
      961 gctgtgcgac ggcatgggcg gcgtgcatgg cggcgagatc gcctcggcgg tcgccgctga
     1021 ctcattcatg cagtatgcgg aagatatgct gcgccgtgaa ccgaaatccg atgcggcgca
     1081 ggtcctgcgc gaagcggcgg catatgccaa tctgaaggta tatgaccgcg ccttccgcga
     1141 cgagagctgc cggggtatgg gtacgacgct tgtggccgcg cttgtgcgtc cggaagacgc
     1201 cgccgtcgtc aacatcggcg acagccggtg ctactggctg gcggacggcc agctccagca
     1261 ggtcacgcgg gatcattcgc tcgtgcagag catggtcgac cgcgggctga tcaccgagga
     1321 tgaggcccgt tcccatccgc gaaagaacgt gatcatgcgg gccgtcggat tggaacgaac
     1381 cattcgcagc gatattttcc ggctggacat ccgtcccggc gacgcgctgc tgctctgctc
     1441 cgacgggctc agcaatcttg ttgacgccgg ggagatggaa tcccttcttc tggcgtcctc
     1501 ggatgacgat gcggtctgcc gtgagcttct gcgcatggcg ctcgaccgcg gcacgccgga
     1561 caacgtcacg ctggcgcttg tgcgccgatg aacctacggg ggaatgatat ggatacgaaa
     1621 aatcaggata aataccgcgg catgatgctc gatggccggt atgagctcgt agagctgatc
     1681 ggcagcggcg gcatggcggt cgtatataaa gcgatgtgcc accggctgaa ccgctatgtg
     1741 gcggtgaaga tcatgcgccc ggagcttgcg aagaacgaga agttccgccg ccgcttccag
     1801 acggaatcgc aggcgatcgc gaagctcagt cacccgaaca tcgtcggcgt gtacgacgtg
     1861 agccgcaccg atcatgtgga atacatcgtt atggaactcg tggacggcat cacgctcaaa
     1921 cagtatctgc aggaccacgg cccgcttgac gcggtgcagg cagtggattt ctcgctgcag
     1981 atcgcgcggg cgctcgccca cgcgcacggg aaaggcattg ttcaccatga tatcaagccg
     2041 cagaacatcc ttgtcgtcaa tgacggcgtc atcaaggtcg cggacttcgg catcgccaac
     2101 cttcagtccg aagtgccgga cgtggagaac gaagccatcg gctcggtgca ttatatctcg
     2161 ccggagcagg ccaggggcct tccggtggac ggacggagcg atatctattc gctcggcatc
     2221 gtcatgtacg aaatgctctc cggaaagctg ccgtttgatg gcgacgatga ccggacggta
     2281 gcgctcaagc acctctcggc tgtcccgacg cctctgcgcg agatcgcgcc gtcggtgccg
     2341 gaagcgctcg aaaagatcgt gatgaaggcg atggaagcct cgctcgataa ccgctatcag
     2401 acggcggacg cgcttgccgc cgatctcgag gcgctgcaga aatcgctcac cggctcgatt
     2461 gacaggatcg agggcaacgt tgcgccgctg agcacggcgg gcgagcttgg cagggaggac
     2521 tatgtccgcc gccgccgccg cgcccaccgc gtcagcatgc tctccggttt tttcggcgtg
     2581 atggccgcca ttttgatcat tgccgtcttt ctctggaact attttctgag cgatatattc
     2641 gttgtcaacc agcgtgtcgc catcccgaac tttgtcggca gcaactacga gaccatcatc
     2701 aacagcaaag aattcaaggg gaaatacaac tttgatctca tttacgagat cgacccgaac
     2761 gtcgagcgcg gtatcatcat cggccagtcg cccgacgcgg gaaagagcta tatgctgact
     2821 tcggacggca tccatgtgac gcttacggtg agcactggcg ttatgttaac cgaaatcccg
     2881 aaccttctca actacgatta tcgcgaagcg acgagcgagc tggaaaaact cggctttgtt
     2941 gtggacaaaa cgtttgccga ttccgacgat gtcgcggcgg actgtgtaat gcagatcagc
     3001 ccctcgccgg gggagaagct ccccgcgggc tcgacggtct atctcacgat cagccaggga
     3061 gccaagactg aaacggtcat catgccgtat ctcgtcggca aggactatgc cacggcggaa
     3121 gcagagcttg ccgcggcgcg gctgacgctc gtttccgtga gctacgttta caacaacacg
     3181 atccccgtgg actatgttgt cagccagact gtcgaggccg ggacggagat ccctgcgtac
     3241 tccaaaatct atctgcagat ctcgctcgga cccgaaacga cgccgacacc ctcgccctcg
     3301 ccggtcccga cgcaggatcc gaacgccgag tgacgcgcat ggaaggacgg atcatcaagg
     3361 cgctgagcgg ctactactat gttgataccg gcgaggggct cgtcacctgc cgcgcccggg
     3421 gaaagtttcg tctcgacgga acgagcccgc tcgtcggcga ccgtgtgcgg ctggatgtgt
     3481 catcggacgg cacaggcagt gtgcgggaga ttctcccgcg gcggaattat ttcattcgtc
     3541 cggctgtcgc caacatcgat ctcatggtga tgctcgctgc ggcggtcaat ccggtcacgg
     3601 acccgtttct catcgaccgc gtatctgcgc tcgcggcaca tcattcctgc gactttcttc
     3661 tctgcatcaa taaggccgat ctcaattccg gcgacgagct tttctccatt tattccgcct
     3721 caggcatccc cgtgctgcgg acgagcgccg tcaccggcga gggactcccg gagctttccg
     3781 agcggcttgc cggaagagtc tgtgcattta cgggcaactc cggcgtcgga aaatccagcc
     3841 ttttgaacgc gctttcgccg gagctttccc tgcttaccgg cgaggtgagc cagaagctcg
     3901 gccgcggccg ccacacgacg cgccacgtgg agctttttgc tctgccgaac ggcgggtacg
     3961 tcgccgatac gccgggcttt ggctcctttg atatcgagca gatggaatcc atccgcccgg
     4021 cagagttgca atattgcttt ccggagtttg agccgtatct cggggcgtgc cggttcacgg
     4081 actgcacgca tcggaacgag cccgactgcg ccgtgaacgc cgcggcggac gagggggcga
     4141 tccatccctc acggctcgac tcctaccgcc gtctgtggga gcaggcgaac cggaaaaagg
     4201 actgggaagt ccgccaaacg gaataagaac atatggtccc ccggtgcgta ctatggaata
     4261 aacaaggtgc gtatcggggg actgtttatg cggcgcggaa atcggcgaaa aaaaccggtg
     4321 ggatggatca tcgtaacggt gggggtcatt gtcctgctct cacagatcct gccggagggg
     4381 ttctggtggt ttttgctcgg cgcgtgtctg atcggcgtcg gcatatgtat caatcgaaat
     4441 tgctgttgag gaggatcgta tatgtatatg aaggtcattt atatccggct tccgggattt
     4501 ttgtatcgga tgctttcgaa gctgtgacat atttttcggg cgttcctcat acggtatatt
     4561 gaaaacggac aaggagagga atgtctatgg aagtcaatct gaaacgggaa gccattcaat
     4621 actgtactcc tgtttattcc gatacactca gccgcgagga gacgctcgaa acggtcgttt
     4681 ccgatacgct gccggacgtc gcccgtattc tggacgtgga tgccgcggtg tatctgcgct
     4741 ccaaaacgct gctgcagggg agcatccgga tcgagggaag catccgcgga acggttctct
     4801 atatcggcga ggattcggag cggctgcaaa ggatcgaagc ggagctgccg gtcagctttt
     4861 ccgcggaggc agagggcacg gaggataccg atatacttgc cgcgaatctc tgcgtatcgt
     4921 cggcggacgc caggctttta aatcctcgga agatccagtt tcgcgtcggg gtctgcgccg
     4981 cggtcgcggc gtatcgctgc gtgacagcgg agctcccgac agaggcggag ccgacgccgg
     5041 gactttatac gctgaccgac acaaagacgg tcagctttct ctccggcatc gaggagaaga
     5101 actttcttgt ctccgacgag gcagagcttc ccgccggatg cccgccgatg gaaaaactgc
     5161 tctgctattc gcatacggag actgtggagg agctcaagca ggtcggcgga aagatgatat
     5221 tgcagggaac ggttcggctc gatacgctgt atctgacggc cggagacgat gcgccgcagc
     5281 agcagagctt tcgcatccca ttctcccaga tccttgacat agcgaacgga aaagcggagc
     5341 tttccaccgt gatattgacc tccggcgggt gctatctcga acctctctcc ggcagcgacg
     5401 gcataacctc gctgcatttg gagctgcacc tgtgcgcgca gacgctctgc cgcagcgaga
     5461 cggagatccg gtatatcgcg gacgcctaca gtctcaaaaa cgcctgccgc ctgaaatcag
     5521 acgtcatccg cacgctgaat acggcgcgag cgtcggtcct tcggcagtcg gtgcgtgaga
     5581 cgctggaagc accggattcc ccgaaggaga tcgtgtatac ccgcgtgacg gcggcgtttc
     5641 ctgtccggac ggagaacggc gcttcggaaa acatctgcgt ccgcgtgctc taccgcaccc
     5701 agcgcggcag tctctcgtcc atgagccgcg ttctcacgct cacgtttgag ggcggcggga
     5761 caaccgggat gacgctgccg gaaacagcac gcttcggcga ggtgtacgcc gccgccggag
     5821 acggagctgt ggagctgcgc tgcccggtcg aactctcact cgacgcggcg gacgtttccg
     5881 agctcaccta tgtgactcaa atggaactgg aggaagggga gaccgcggac gagaccgagc
     5941 gcccgtccct gatcgtgatc cggctcggcg aggaatcact ctggacggtc gcaaagaaat
     6001 tcggctctac gcgcgagctg atcgaatcgg cgaatcccgg gccggtgaac ccgggggatc
     6061 tgatccttat tccgcacggg agataaggaa cccccttgtc atccggcaac gaaagtgcta
     6121 taatttcttt aaacgtattt ttgtttgggg gaattacagc atgtccggac attccaagtg
     6181 gcataacata cagaaaacca aaggcgccca ggatgccaag cgcgcacagg cgttcaccaa
     6241 gatcgcccgt gaaatgatcg ttgccgttaa ggaaggcgga agcggcgatc cggcgaacaa
     6301 ctcccgtctc gcggcggtca tcgccaaggc gaaggcgacc aatatgccga acgacaacat
     6361 caagcgcacc attgaaaagg cgctcggcag cggcgcgacc gacaactacg aaagagttac
     6421 ctacgaaggc tacggcccct ccggcgtcgc catcatcgtg gacgccatga ccgataaccg
     6481 caaccgcacc gcgtccgatg ttcgccatta ctttgacaag tacggcggaa acatgggtgc
     6541 cgccggctgc gtttcgtggt cgttcgataa gaagggcgtc atcgtgatcg ataacgagga
     6601 cgaggatctt gacgaggaag ccgtttttga atcggcgctg gaggccggcg cggacgatct
     6661 cgaagcggaa gagggcatct tcacggtcta tacccagccg gacgatgttg cctccgtcgc
     6721 cgaggcactc tccgccaagt accgtctgct ctccgcgcag gtggaaatgg ttccgcagaa
     6781 ttacatcacc ctcaccgacg aaggcgatat caagaacatg tccaagatgc tcgagatgtt
     6841 cgacgataac gaggatgttc agaacgtctg gcacaactgg gagaacgaag agtaattccc
     6901 gccatataac aaaaccccga ctgtctgcgg acagtcgggg ttttggctta tgtgtgccgt
     6961 ttcaggtatt ctacgatctc cggataccgg tcggactgga tgtggattcc gtcgccggag
     7021 ttgaagctgt tgagaaaata tccggccttg tcgcacatca tgcggaaaag atcaatgtat
     7081 tccgtgtcat attcacggga gagcgttttg acgagctggg aataaaacac cgtactgtca
     7141 ggattgtaaa gctcgttcat tggtgtgtag cgcccgacgg gaacgagcga gagaagatag
     7201 atcttcgcgc ccggctgcgt tccttttaca agatcgagga tcgtccgcat ggggttcatg
     7261 aaccgttcca cacggtcgtc acgcagcgaa ctgtcgttga tgcctagcat tatgtaaact
     7321 tttccgtagg acttggcgtg cagcgcgtcg ctgagcgtgc cgtatcgtcc atcgggcagc
     7381 tcatagaatt cgtaatctac aacggcctgt gccgtgtggc cgacgacggc gtagtaatcc
     7441 ggaccggata ggccggagta tttctgcatg ccgacgatct gcgaatggcc gatgaagcac
     7501 acatcgttaa accaatcgtc cgataccggc tcgctctccg caacggtgga gacggggagc
     7561 gcggtcagga tatcccgctg ggagccgaga aagcggagga tgatctcctc gccatcggcg
     7621 agcggcacgg cgtcgcgata gtggaagtat ccgtccgtat cctcgatcat cacaccggcg
     7681 cgctggatgg tgaaggcggc ggtctgcgcc tccggtgtca tcagcccgcc gtcgaggaac
     7741 gagtaccagg cgttgatcgc ggggagagac acgccgtagg cgttagcgca gcggtagagc
     7801 gtgatgagaa acgcctcgcg cgtgatcggc atatcggggc tgaatgtacc gtctccgtcg
     7861 ttcgggatga tgtttttctt cagcgcccac gccgttccgg cggcgtacca ccgggaagcg
     7921 tccacgtccg aggggatcgt ttccgcgtcc tccgcgctgc cgaaaatgtt atagagaata
     7981 ccggcgagca tcgcgcgcgt catgtctctg tccatcccgc cgctttccgc gtcgcggaag
     8041 agctcgcgat agtcggacat cgaggcggcc tcgcagtatt cggatacgcg c
//
LOCUS       DAWAAE010000091         8049 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3976_length_8049_cov_2.960345, whole genome shotgun sequence.
ACCESSION   DAWAAE010000091 DAWAAE010000000
VERSION     DAWAAE010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_8049_cov_2.960345"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1504 a         2578 c         2424 g         1543 t
ORIGIN      
        1 tcaacgtcgt cggcaccacc gtagcaaagc tcggcgatta tgtcatgtac acctgggccg
       61 gcccggagat cgccgttgcc accacgaagg gctacaccac gcagatcgcc gtgctcgatc
      121 tgctcgccgt atggatggcc aacgagcgcc ggacgctcac cgcgccgcgg tacgccgagc
      181 tcgtcgccgg cattgcggac ctgcccgagc gcacgcagcg cagcattgac ctgaacccgc
      241 aggtcagcta tcttgccgag cgctactgcg gcaacagctc gctcttcttc atcgggcgca
      301 acatcgacta cgccgtcgcg cttgaagcct cgctcaagct caaggagatc tcctacatcc
      361 actccgaggc gtatgccgcc ggagagctca agcacggcac catcgcgctc atcgatccgg
      421 gccgtctcgt cgtttcgctc gcgtgctacg aggagctgtt cgacaagacg atgtccaaca
      481 tcaaggaagt caaggcgcgc ggggccaagg tcctcgccgt ggtgaacgag ggcaaccgcc
      541 gtgtgttcgc cgaggcggac gacgtgctct ttgtccccgt gaccgacccg ctcttctccg
      601 ccattccgga gatccttccc ctgcagcttt tcgcctatca tgtcgcccgg tgcaacgggt
      661 gcgacattga taagccccgc aatctcgcca aatccgtcac cgtggagtga gtttcatgaa
      721 agacgtttac gaaaatcccc tctgctcgcg ctacgcctcg gagcagatgc agcatctctt
      781 ctccccgcag tggaagtttt cgacgttccg ccgtctctgg ctcgcgctcg ccgagagcga
      841 gcaggagctg gggctgtcca tcacggacga gcagcttgag gaaatgcgcg cgcatctcga
      901 cgatatcgat tttgaccgtg ccgccgagta tgagcaccgg ctgcgccacg acgtgatggc
      961 gcacatccgc acgttcggcg acgcctgccc gaaggccgca ggcatcatcc atctcggcgc
     1021 gacgagctgc tatgtggacg ataacaccga ccttctgcag atgcgggcag cgctgaagct
     1081 catccgcggc gaattgctcg cggtgatcga cgcgctttcc gaatttgccg cgcgcgaggc
     1141 cgacacgccg acgctcgcct ggacgcactt tcaggccgcg cagcccacga ccgtcggcaa
     1201 gcgcgcgacg ctctggctgc aggattttct gctcgactta gagcagctcg aaacggagct
     1261 ctcccgcctt cccatctccg ggtgcaaggg cgcgaccggc accgcggcga gctttctcgc
     1321 cctcttccac ggcgacgggg agaaggtcct cgcgctcgag aagaagatcg cggaaaagat
     1381 gggcgttccc aactgcatcc ccgtcgccgg gcagacgtac acgcgaaagc tcgatttctt
     1441 tgtgatgaac gtcctctccg gcatcgcgca gagcgctgca aaaatggcga cggacatgcg
     1501 ccttctcgcg catctgcgcg agctggacga gccgtttgag accgagcagg tcggctcctc
     1561 cgccatggcc tacaagcgca accccatgcg ctgcgagcgc gtgtgctcgc tcagccggta
     1621 tgtggtaaat ctcgcctcca acaccgcgga caccgccatg acacagtggc tcgagcgcac
     1681 gctcgacgat tcggccaacc gccgtctcac gctgccggag ggtttcctcg cgtgcgacgc
     1741 cattttgacg ctctgcgcca acgtcgtgcg cggctgcaag gtatatccgc gcgtgatgga
     1801 aaagcacctg cgcgaagagc tgccgtttct tgcaaccgag aacatcctca tgcgcgctgt
     1861 atcgctcggc gaagaccgcc agaagctgca cgaggtcatc cggcagtatt ccgtgcgcgc
     1921 ggcggagagc gtcaagctcc gcggcgagga cgggcatctg ctcgatatgc tgctcgcgga
     1981 cgagcggttc catttaacgc cggaagctct ggaggagctt ctggatgtcc gccagttcgt
     2041 cgggcttgcg ccggagcaga cgcgcgcctt tctcaaaact catgtttatc ccgtgctgga
     2101 agcgcgccgc ggcgatatcg ccgggagcgt tccggtgcgt gtctaaaaaa gcaaggagga
     2161 tacaaaaatg cttactgctg ttgttggaat caactggggc gacgagggaa aaggccgcat
     2221 ggtcgatctt ttctccgccg agcatgagat cgttgtccgc tatcagggcg gcaacaacgc
     2281 cggtcacacc gtcgtgaacg agcgcggcaa attcgttctc aatctgctgc cgtccggcat
     2341 tctgcgcccc ggcacggtga acgtcctcgg ccccggcatg gtgctcgatc ttgagcatct
     2401 cacaaacgaa gtcgcgcaca tccgcgagca gggcgtggcg atcacgcccg agcatctgaa
     2461 gatcagcgac cgcgccacca tctgcatgcc gtaccacaag atgatggaca ttcttgaaga
     2521 ggaccgtctc gcggacaaga agttcggctc cacgcgccgc ggcatcggcc cggtgtacgc
     2581 cgacaagtat atgaagaaaa cgctgcgcac cggcgatctt ctcttcccgg acaagctgcg
     2641 cgaaaagctc gccgatcttg tcgagtggaa gaacatcacc gtgcagggct acggccacga
     2701 gccggtcagc gttgacgaga tgtacgcctg gctcgagaag tacgccttcc cgtggcgcga
     2761 gtatctctgc aacacgaccg aatacctctc cgcgcaggcg gatgcgggca agagcatcct
     2821 ctttgaggcc cagctcggcg cgctgcgcga cattgacttc ggcatctacc cctacacctc
     2881 gtcctcgtcc acgatcgcgg cctacgcccc catcggcgcg ggcatcccgg ccaagcggct
     2941 ggacagcgtc gtcggcatca tgaaggcata ttcgagctgc gtcggcgaag ggccgttcgt
     3001 gtgcgagctc ttcggcgagg agggcgaccg tctgcgcgaa gccggtgccg agtacggcgc
     3061 ggccacgggc cgtccccgcc gggtgggtgg gtttgacgtc gtcgcctccc gctacggcgt
     3121 gcagatgcag ggtgcgaccg agctcgcgct cacgaagctc gacgtgctct ccggctggga
     3181 gaagatcccc gtctgcaccg gttatgagat agacggcaag atcatccaca ccttccccgt
     3241 ggagaccgtg cttgagcgct gcaagcccgt atacgaatac gttgagggta tccgtgggcg
     3301 cgggccgaga ggaatatatc cgtctatgaa aaaactgcgc gtatttttcg cgtgcgtcac
     3361 gagccgcctt gcgcggctcg cgctgcggct tttgggccgc ggcggcacag cgctgcccgg
     3421 aaaggcagcg ctgcgcgtat atccggggct gatgcgtgag ctgggacgcg gcgtggaaac
     3481 ggtgatcatc accggcacga acggcaaaac gacgaccgcc ggtatgctcc gccatatgct
     3541 cgacaaggcc gggacggcat atttctccaa ccgctccggc gcgaatctca cgagcggcat
     3601 caccgcggaa ttcattgaaa acgccgatct tctcggcaag ccgaagaaaa cgctcgccgt
     3661 catcgagtgc gacgagggca atttccccgc cgtcgccgcg gcgctgcagc cgcgcgcggt
     3721 gatcgtgacg aatctcttcc gcgaccagct cgaccgctac ggcgaggtga cgcacacacg
     3781 cgatctcatc gcttcgggca tccagaaggc ccccggcgcg gtgctctgtc tgaatgccga
     3841 ctgctcgctc accgcgtcgc tcggctggga cgtgccgaac aagaccgtgt acttcggtct
     3901 cgagaatatt tcgagcgacg ccgtgccgag cgtttcggac gcgccgcact gtctgcgctg
     3961 cggcgcccgc tacgcctacc ggcgatacac gttcgcccat ctcggcgact ggtactgccc
     4021 cgcgtgcggc gcgcagcgtc ccgcgccgga catggccgtg gagagcgtgg aagccctccc
     4081 cggcggcggc agcgccgtca cgatgaaaac gccggaaggg acggtctcct ttgatctcgc
     4141 gctcccggcg ctctacaatg tctgcaacgc cgccgccgcg ctcaccgccg cgcgcgccat
     4201 gggctgggat atggacatct gctgcgccgc gctcgccgat ttcggcgcgg tgttcgggcg
     4261 gatggagcag ctcaccgtcg gggaaacgag cgtccgcatc gtgctcgtga aaaaccccgc
     4321 cgggtgtgac cgcgcgctcg aatttctcgc ctcgcagagc gaggatcttc tccccatctt
     4381 ctgtctgaac gacaacgccg ccgacggcac ggacgtttcc tggatctggg acgccgatta
     4441 cgaaagcctt ttggccgcgc ggcagtatgc gtccatcggc gtttacggcg tgcgcgcgca
     4501 ggatatgcgt ctccggctca agtacgccgg ggcggacgat gccgccatca ccgtatacga
     4561 cacggcggac gatctcgccg cggcggtgca gaaagccgga aaacccgtcg tcgtgctgcc
     4621 gaactacaca tccatgctca ccgtgcgcga caagctgtcc gcactcgccg gcggagggag
     4681 gttctgggaa taatggaact gaacatctgc catctgtacc cggacgtgct caatctttac
     4741 ggcgaccggg gcaacatcgc gtgcatgaaa aagcggctgg aatggcgcgg catcggctgc
     4801 gccgtcacgg agctgccgct cggcacggca gacgatctca ccgggtacga tctctttttc
     4861 atcggcggcg ggcaggactt tgaacagacc gtgcttctcg ccgatctccg cgccgggcgc
     4921 gcggagaaca tccgcgccgc ggcggaggac gggaaaacgc ttctctgcat ctgcggaggg
     4981 tatcagcttc tgggcaacgg ctaccgcacg gcggccggac aatggtgcga ctataccggc
     5041 attctggact ttacgaccga aggctcctac gagcggctca tcggcaacta tgcctaccgg
     5101 ctcggcgaag agtccggcgg cggcatcgtc atcgggtttg aaaaccacgg cgggcgcacg
     5161 cgcctgggcg cttcgctcac gccgctcggc acggtgctgc gcggtcacgg caacaacggc
     5221 gaggacggca cggagggcgt gcggtacaaa aacgtgtacg gcacctacag ccacggtccg
     5281 ctcctgccga agaacccggc gctctgcgat gagatactgc gcggcgcgct ctgccgcaaa
     5341 tacggcgcgg ccgagcttgc gcctctcgac gacagtgccg aggaggccgc ccggcaggtc
     5401 atgctcacaa gactgcacat tctctaaagg gagggcattc attatgcgca actatgaaca
     5461 ggaactcgaa aaccgcgttg cctttatccg gcagctgctg aaggaaagcg gctgcgaggg
     5521 catcgtttac ggcaacagcg gcggcaagga cagcgcgctc gtcggcattc tctgcaagaa
     5581 ggcgtgcggc aataccgtgg gcattctcct gccctgcgcc acaacgcaga actacggcag
     5641 cgacacggac gacgcgatcc gcgtcgccga gcagtatggc atcgcctacc gcaccgtgga
     5701 tctctcggaa acgcgcggcg cgctcgtcgc ggcgctggaa aaggcgggcg tagagctcaa
     5761 caacgccgcc gtatcgaaca ttgccccgcg ccttcgtatg acggcgcttt acgccgtcgc
     5821 tgccgccgag caccggctcg tcgccggaac gggcaaccgc agcgaggcgt tcatgggcta
     5881 ctgcaccaag tggggcgacg ccgccgccga cttcaacccc atcgccgatc tgagcgtgac
     5941 cgaggtcttt gaattcctcg actatctcga cgcgccggag accatccggc acaaggctcc
     6001 ctccgccggg cttttcgagg gacagacgga cgagaaggaa atgggcgtct cctacgccgc
     6061 catcgaccgc tatatggacg gaggcgccgt gaccgacgcc gagcgcgcga tcatcgagcg
     6121 cttccaccgc aacagcgagc acaagcgtgt cggacgaaag gattaccgcg ccaactgaat
     6181 ccatgcctat gcaaaagggt gctgaccggc ttggtcagcg cctgtcggct ttgaaaacca
     6241 tcacaagggg gcaatttcgt ttggtggaaa atcttcttcg tgcttttcgg cgttttacat
     6301 cctttcagat catcatactc ggctttgccg gagtgatcgt tgcaggtgcg ctgctgctga
     6361 cgcttccggc ggcatctgcc gcaggggcag ccacgccgtt caccgacgcg ctttttactt
     6421 cgacatcggc ggtctgcgtg accggtcttg tcgtccgcga cagcggcagc tactggtctc
     6481 cgttcggaca gagtctgatc cttctgctga tccagatcgg aggtctcggc gtcatcacaa
     6541 tgtcggcaac ctttctgatg cttgccgggc ggaacatttc cctcaaggaa cgaagcgcca
     6601 tgcaggatgc gctttccgct ccgtcggtcg gcggcattgt gcgtttgacg cgttttattt
     6661 tgaaagggac ttttatcgtt gagctcaccg gcgcgcttgt catgctgccg gttttctggc
     6721 gggatcacgg cttccgcgga atctggatgg cggtattcca ttccgtctcc gcgttctgca
     6781 acgccgggtt tgacattctc ggcacggcag aaaggccgta tccttcgctg acagcctatg
     6841 tcgaaaaccc tgtcgtcaat atcaccgtga tttttcttct cgtggtcggc gggctcggct
     6901 ttctcacctg ggatgatatc cgaacgcacc ggtggcggct gtcccgctat cggatgcaga
     6961 gcaaggtcat tcttgcaacg accgccattc tgcttttttt ccccgctgtt ctgttcttct
     7021 ttacggaata ttccgccttt ccgctgcaaa agcgtattct tgcttcgctc tttcagtctg
     7081 ccacaccccg gacggcgggc tttaacacga cggatcttgc cgccatgagc gacgcatccc
     7141 gcgccgttat gatccttctc atgctggtcg gcggcgctcc cggatcaacc gccggcggaa
     7201 tgaaagtcac gacgcttgcc gtgcttgcgg caaacgtaca ttccatattc cgacagcagg
     7261 aatcgccgca gctttttcac cgccgcgtcg acagccgcat tgtaaaaagc gcttctgccg
     7321 ttttgctgct gtatttgacg ctgtttctca ccggcgggtg tatcatcagc gcggcagaag
     7381 atcttccgct tgccgtctgc ctgtatgaaa cagcatccgc catcgggacg gtaggcttga
     7441 cgctcggcat aacgccgcag ctcggacagc tttctcattg gatactgatc ctgctgatgt
     7501 tctgggggcg cgtcggcggt ctgacgctga tctacgctgc cttcgccggg cacaatcggt
     7561 atcatgcagt gtatccgaaa gaaaacatta ctatcggata atcgtctttt tcaaggagga
     7621 tagaatatga aaaacattct tctgatcgga ttgggacgct tcggacgcca tcttgccatg
     7681 gagctgaacc gtctcggcca tcaaatcatg gcagtcgaca gggacgagga gcgtgtcaac
     7741 gagtgcatgc cgtttgtgac caacgcgcag atcggcgaca gcacacgatt ggaatttctg
     7801 cgctcgctcg gcattgggaa ttatgacatc tgctatgtta ccatctgcgg cagctttcag
     7861 gattctctgg agacagtttc gcttctcaaa gagcttggcg caaaaaccgt tgtcgcgcgc
     7921 gcggatcggg acgttcagga aaagtttctg cttcgcaacg gcgcggacta cgtcacctat
     7981 cccgaaaaac agcttgccaa atggtcggcc acccgctaca ccgccaacca tctgttcgac
     8041 tacattgag
//
LOCUS       DAWAAE010000092         8043 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_3980_length_8043_cov_2.966700, whole genome shotgun sequence.
ACCESSION   DAWAAE010000092 DAWAAE010000000
VERSION     DAWAAE010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3980_length_8043_cov_2.966700"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1544 a         2360 c         2578 g         1561 t
ORIGIN      
        1 gtgcgaggcg gtgcgcgaaa agctcgcggg gatcgcgcgc gtgacgctgc acgcgccgtt
       61 caacgagctt ttcccctgcg ctatcgatcc gcgcgcgaga gcgctcgccg cggaccgctt
      121 ccgtgaggcg atcgctcttg caaagggcta cggcgcggaa aagatcgtca tccacggcgg
      181 ctataatccc caaatgtact atccctgctg gtatacggag cagtcggccc ttttctggaa
      241 ggattttctg cgcgaagacc ccggcgtgga catcgtgctg gaaaatgtgc tcgaggaaga
      301 gcccgagtat ttgccggaca ttgtccgctc ggcggatcat ccgcggctgg ggctctgcct
      361 ggacatcggc cacgtgaacg cctattctgc cgttccggtg atcgaatggc tgaagcgctt
      421 cgcgccgtat ctccggcatt ttcatctgca caacaacgac ggctcgttcg atgcccacgc
      481 gccgctcgac gagggcggta taccaatgaa ggaatttttg cagagagcgc ttgcgctctg
      541 cccggcggcg acctttacac tcgaactgca ggaggacacg gcgcgcggcg cgcgctggct
      601 ttttgaaaac ggatttatca ccaaggagac aaacacatga cactcagaga atggacagca
      661 aagatcacgc cgccggacgc tgcggccatg gaagaggcgc gccgccgtca ggcgcagctt
      721 gccaagcccc ccggcagtct cgggcggctc gaagacctct ccgtgcagct cgcggggatc
      781 accgggcgcg tgcataacaa gatcgaaaaa aagcatcttc tcgtttttgc cgccgacaac
      841 ggcgttgtgg acgagggcgt ttcgagcgcg ccgcgctccg tgacactctc gcagacgatc
      901 aaccttacgc gcggcaagac cggcgcggcg acgctctgca agcatttcgg ctgcgagatc
      961 accgtctgcg acgtcggcgt cgcagcgaac atccgcgaaa aggcggtgct atgccgcaag
     1021 atcgcctacg gcacgaagaa tatcgcccgc ggcagcgcca tgacgcgcga ggagtgcgag
     1081 cgcgcggtgc tcaccggcat cgagcttgcc gcggaaacgg acgcggacgt gctcggcgtc
     1141 ggcgagatgg gcatcggcaa tacgacaacg tcctccgccg tgctcgcggt gctgctggac
     1201 gcgcctgtgg agaccgtgac gggtcgcggc ggcggcgtaa cggatgaagc gttcaccagg
     1261 aaaaaggccg tcattcaaaa agccattgcg atcaatgcgc cggacagaaa cgacgctctc
     1321 gacgtgctcg caaaggtcgg cggctttgat ctcgccgcca tgtgcggcgc attcctcggc
     1381 gcggcggcga cgcgccgccc ggtcgtgatc gacgggctca tttccgccgt ggcggcgctc
     1441 tgcgcctgcc ggatctgccc ggacgtgcgc gcgtatcttg tgccgagcca tgcctcctat
     1501 gagatcggct ataagctcgc catggacgag atggggctgg agccgatgct gctgctcggc
     1561 atgcgtctcg gtgagggcag cggctgcccg ctcgccttcg aggtgctgga cgccgcctgc
     1621 gccattatca acgatatggc gacgtttgac gaggccggca tcgatgacgg ctatctcgac
     1681 gagatccgcg agggcgataa gttcaccgtg gagggcgcgc aatgaaagca ttcatctccg
     1741 gcggcgcgaa aaacggaaag acgacgctcg gacaggatct cgcggtatac ctcgccgcgg
     1801 gggaaaagca ttactatgtc gccaccatga tctccgccga cgccgaggac gacgagcgca
     1861 tccgccgcca catcgccgac cgcgcggggc tggactttga aacgatcgag tgcccgcggc
     1921 acatcctctc gtgtctggat accgccgaca gaaacggcgt attcctgctc gacagcgtca
     1981 cggcgctgct gcaaaacgaa attttcccgc ccgagaaaaa ctacgcgctc gatctcccgg
     2041 cggcggagcg gtgcgcgcgg gagctgctgg aatttgtccg ctcggtgcga agcgtcgtgc
     2101 tcgtgagcga ctatatcttc tcggacgccg gggagtatga ccccatgacc gagcagtacc
     2161 gccgcgcgct cgccgggatc gaccggatgc tcgcgcgcga atgcgatacg gtcgtggagc
     2221 ttgccgccgg gcggcgcatc gtccataagg gggtgctccc gttttgaaaa gatatctgca
     2281 cgctctggcc atgtgccgga gcatgttctg cgcgatcccg tgcttttggc gcgtgtggga
     2341 tgaggaagcg cgcgataaaa tgcttctgtt cctgcctgtc gtcgggctgg agatcggcgc
     2401 gatctgggca cttctcgcgt ggctctgccg gtatctggcg ctcccggcgc ttgtgcaggg
     2461 cgcggtactc ggcgcatacc ccttcgtcgt gacgggcttc atccatctcg acggcttcat
     2521 ggacgtgacc gacgccgtgc gctcctgccg cgatcttgcg cggcggcgcg aaattttgaa
     2581 ggactcccgc gtcggcagct tcgccgtcat cggcgtcgtg ctgctcgcgc tgtgtcagac
     2641 ggcgctcttc gcttcggcaa agacggcggc gagcctctgg acgcttgtgc ttcttccggc
     2701 agtcagccgg tgcggttcgg cgctcgccgt aacggcgctg cgcccaatgt ctacgagcca
     2761 gtacgccgcg cagaaaaagc cgaagtcgca catcatcgtt ctctgtgctc agctcgcggt
     2821 ttatctcgcg gcgggctttc ttgccgggtg gcgcacgggg gcggcgctct gcggctgtct
     2881 tgccaggtac gcgctcgcgc tccggaaagc gttccgctcg ctcgacggca tgaacggcga
     2941 catttccggc tatgcgctca cgctctccga gctctgcggc gcggcggtat gggcgctcat
     3001 ctgaaagaaa aggggaaaca ccatggtttt tattttcggc ggggcctatc agggcaagac
     3061 cgattttgcc aaagaagcct tctctctcgc ggagagggac atcttttcct gctccggcgc
     3121 ggagatcgat ttctcccgcc ggtgcgtgga taatatcgaa aacttcacgc ttgcctgtac
     3181 ccgcgcgggg atcgatccgg tcgagtattt ccgcgtgcac cgcggcgagt gggaagagag
     3241 catccttatc tgccgtgaca tgttctgcgg cgttgtgccc atgggcgcgg aaacgcgcgc
     3301 gtggcggcag gataccggac ggcttacgca atatctgagc cgcgaggccg agcatgtgag
     3361 ccgcattttc tgcggtctgg agcagcggct gaaatgagga tccttctgct gcgccacggc
     3421 atcaccgagg caaacgaaaa gcacctctac tgcggcagca ccgatctgcc gctctctccg
     3481 gcgggccgcg ccgcgcttcg gcggcaggaa atgccagcgc cgggcacacg gtttattacg
     3541 agcggcatgc gccgctgcaa tgaaacgctg gaagcgctct tcggcaccgt gccttatgag
     3601 atcgagccgg acttccgcga gatcgacttc ggcgattttg aaatgaagag ctacgaaatg
     3661 ctgaaggatg atcccgctta tcaggcgtgg ctcaccggcg ataacgagaa gaacgtcccg
     3721 ccgaacggcg agagcgggga gcatatgaca aggcgcgctc ttgccgcctt cgcgcgtgta
     3781 cagaaagccg ggcgcgacgc cgtgatcgtg acgcacggcg gcgtgatcgc agcgatcatg
     3841 gcgcaccttt tccctgaggg aaaaaaccgc tacgagtggc aaagtccgaa cggcggaggg
     3901 tatgtgatcg aagacgggga atatacgccg ttttctccgc gatgaccggc aaaaagccgt
     3961 tgaaagagcg gccttttgcc tgtataataa aaccgttatg aacgtttatg tgctggacat
     4021 tgcgccgctg cgcggccgcg agcgggaagc gctcgcactg ctgccggaag agcgccagga
     4081 aaaggcgctg cgcatccgga gagaggaagc gcgtctgcgg agcatcggcg cttcactgct
     4141 gcttcgcgct gcggggatcg aaccgccgtt tgcctgcggc gaacacggaa agccgtatat
     4201 ccccggcggg ccgcattttt cactcacgca cagcggcacg ctcgccgcgc ttgccgtgag
     4261 cgacactccg gttgggctgg atattgagaa gatcgcgccc gtgcgccgcg ccgcggcgcg
     4321 cgctctcacg atcgaggagc gggactatat gaaggccgat ccggaacggc ggtttgccta
     4381 tctctggaca cgcaaggagg ccgtgctcaa atgcaccggc gcgggactct cgcagccgat
     4441 gaacgccttc tccgtgctgg gggatacggc gtcgcccgag ggcgcgctac tctcgcttta
     4501 caccgtggaa tacggaaact atatgctgtc ggcggctgcg gcgcgctcgg cggtatttac
     4561 gccgcagcgc gtcgatgctc tggagcttct gtaaggagga tacgctatgg aactgagaga
     4621 aaccggaaaa gcgggcgcgg cagccgtgct gctctggccg gatgacggcc tggacgccgc
     4681 cgtatttgcc tcggtcgtcc gctcgctggg aaaatcctgc cgcgtgctcg ttcccgtgtt
     4741 tgcgccggag gaaccgccgg atgcgcgtgt cgccgcggtg gagagcgctc tgctcgccgg
     4801 gtacgacggg cgcatctggg gcgcttacgg gctgcgcggc ggaggaagtg cgcttctctc
     4861 gcttcttgca gagggcaagg tgcgcgtgcg cacctgtgtc gtggaaggcg cggtggaggt
     4921 ccccgcgcag gggctgcggg aattttccgg tacgctcttc cactggaaag gcagcaagga
     4981 taagggcgcg ggaaaatcgt gggaggcgct gcacaaggcg tttccggcgc tgcgctcgct
     5041 cacgctgcga aagctcaagg cggggcagga cgtcgtatcc atccggccgg atattatgac
     5101 aaagcggctt ttgaaagcct tcggcagcgc gggcacggtc cgcgtgagca cgctcgtgcc
     5161 gcacagtgcc tcgtgcgtct ggcggcagct caaccgccgc ccggcgggaa agtcgctcgg
     5221 atggctccaa acgatgcagc cgctccgccg caccgacgag gatcggacgc agatcatcga
     5281 gggcgcggca aagggcgttc cgctctggag ccacatgacc cgcgtggagc cctgcgggga
     5341 gtacggcgcg gtgtgcgtcg atcaggtgga gatctccgcc ggagcgctta ctccggctgt
     5401 gatgcgcgct gcggaaatct atctgaaagc ggtacagaag agcagaaacc gccaaatgag
     5461 aaaagagtga cggattataa aaaaatcgga aacagatgca ctgtttccga tttttttgcg
     5521 aaatttgttc atttacctat tgaatgttcg gctcttgtgg attataatca ccagtgtatg
     5581 ataaataaca aacacacata ggaggaaata gataaatggc agaacgggaa accgaggacc
     5641 gtcgggtacg gaaaacaaaa aaacagcttc gcggcgcgct tacgtcgctg cttctcgaaa
     5701 aggacatcag ccgcgtcacg gtgcgcgacg tggccgatct cgcggacgtg aaccgcggca
     5761 cattttacgc gcactacagc gacgtgtacg atcttctcca tcagcttgag gacgatctcc
     5821 tgcgccgtct ggacgatgtc ggcagccgcc acaacgcccg ccagtcggac gggaagacgt
     5881 tccggtatct gaccgatctc ttcacgcttg cgagcgagta ttcggacatt ttctacacgc
     5941 tttactgccg cagcggagat tccgaatttc aggacaagat attcaacaag ctcaagtacc
     6001 agtacctcta tgagttcctg tccatcttcg gcgggagcga gtccgaaaag ctggactact
     6061 gcgcctcgtt catcgtggcc ggcatgtgca cgctcgccaa ggtgtggatc gaaaacggca
     6121 tgcgcgaaac gccggaggaa atggcgcgtc ttggcggcgc gttcgtgatg cacggcgtcg
     6181 aaatgctcca gtgatatgaa catcgtccat gttctggaag cgggcatgct cgtctgcttc
     6241 ggcatctcat ggccgttcaa tattgccaag tccatccgct cccgcaccgc gaagggcaag
     6301 agtgttgcgt tcgagcttct catcatcgca gggtatctct gcggcctggt cgggaaattc
     6361 atcctcggca atttaagcta tgtggtgttt ttctacattg ccgacattct catggtcgca
     6421 gccgacctcg tcctgacgct ccgcaaccgc cgcctcgacc gcgagcgcga caaagtataa
     6481 tatgaaaaag cgacatcgcc gcctgcaaaa caggcggcga tgtttgtgtg ttttactgct
     6541 ggtttttagg atgacgttcg agcgcttcct tcactgcctt cacggcgttg atgcacgctt
     6601 cgcggtcctt gagacagccg tagatgatgc ggatgtgtgt ggagtcggtg aagcctgcgc
     6661 cggtgctgac gagtacgttt gcctcctgca ccagatagcg catgacctct tcatctgtcc
     6721 cataatagga aatatcgatc cagaaaaaga atccggcctg cggcttgaca tagcggatat
     6781 gcgggatcgt atcgaggatc gagccgagaa tatctgcgcg gatcatccac tcctgccgca
     6841 tgtcctccat gaagcgagta tctttgagcg cggcggtgat gccggcctgc gccatggtgt
     6901 tcgttgcacc gatgaagaga acggctgtct gatggaacac atcggtgatg ctctcgggcg
     6961 cgacgatgta tgcgacgcgg aagccgcaca gacccatgcc tttggagatc gagccaatgg
     7021 tgatagtgcg ctcccacatg ccgggaagcg ccatcatgct gacatactcg tggccgtcga
     7081 atacgctgtc ctcaaagcac tggtcaacga cgcagagaag gtcgtgccgg atgcagaagt
     7141 ccgcgagttt ttccatcgtg ctgcgcgtgt acaccgttcc cgtggggttg ttggggttgg
     7201 tgagcacgac cattttggtt ttaggcgtta cgcgcttttc aaattcatct atatccagat
     7261 cgtagccgtc ttcctgccgc gtcggcacca taacggaacg acctccgatc atcggcggca
     7321 cggcaaagtt gtgcgcgtag gacgggacgg gcatcatgac ctcgttgtcc tcgccgggga
     7381 cgaggaacgg acgcatgacg aatgcgaacg cagagtctga gccgttggat atggtgagct
     7441 cggtacgcgg gttgatcttt acgccgtttt tcttttctac acgttcggca atcgcgtcgc
     7501 acagagcaaa atcgccggtg gggaacgtat agtgggcggc catggagtta agactctcga
     7561 tcacggcggc cttgacgtgc ggcggcacgg tgtcgctcgg gcggtagggg tcggcccagt
     7621 tcatggcgat tggctcgcgg gtgagagttt tctggaaatc aaagccgctc ccgtcgccgt
     7681 gcagcacggc gtattccttt gccatcagtt tgcgaggatc aaacattagt agttctcctt
     7741 tcttatccgg ccattgcgcc gaagatcaca tacagcacgg tcgttatcag cgcaaggatg
     7801 acgcgcggca gcagctgcgc tttgccgagg tcgatggtgt ccacaccggc gcccatggcc
     7861 gattcaatga cgatatctga gagcggggag gtgacgtcgc cgaacgtgga gccgccgacg
     7921 agcgccgcta cgaccagcgg aatgttgccg ccgacactgg cgacgagcgc gagcgcgatc
     7981 ggcccgagca gaaagcatgc gctgatgagt gagccggtgg cataggaggc cacggcgcag
     8041 ata
//
LOCUS       DAWAAE010000093         7955 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4029_length_7955_cov_3.972658, whole genome shotgun sequence.
ACCESSION   DAWAAE010000093 DAWAAE010000000
VERSION     DAWAAE010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_7955_cov_3.972658"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2075..2084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1400 a         2379 c         2528 g         1638 t
ORIGIN      
        1 gaggaacacc ttggggctgc gcacgatggc gcggccgatg gcgacacgct ggcgctggcc
       61 gccggagagc gccttcggct tacggtcgag cagggcggtg atgtccagga tctccgcggc
      121 ctcgcggacc ttcttgtcga tctcggcctt cgggagcttc ttgagcttga gcgagaacgc
      181 catgttctcg taaacggtca tgtggggata aagagcgtag ttctggaata ccatggcgat
      241 gtcgcggtcc ttgggggcga cgtcgttcat gcgcttgccg tcgatgaaaa gatcgcccga
      301 ggtgatctcc tcgagaccgg cgatcatgcg cagcgtcgtg gacttgccgc agccggacgg
      361 gccgacgagc acgataaatt cacggtcggc gatatccagc gagaactgct gcaccgcaac
      421 gacaccctct tcggtgatct gcaggttgtg cttcttctcg ggagcgtcgc cctttttctt
      481 cttcgtgccg ccctgattcg gatagatctt cttgatgtct accagacgta cttctgccat
      541 gtgtaccgat cctgatctga gagcctccgc tgctcagacc ttacctccgc cgatgaatgt
      601 acggaggcta tacggcaggt ctccacactg ccgcaccgcg gatcctgcgg ggaaaacaaa
      661 ctcctttcat cgaaccgcgt accgctcgta aggtggagag cagaagccgt tcggtattgt
      721 atttaacgta ccataaaacc gtttccaacg caagacctgt tatggcattt cgccaaaact
      781 ttacatggtt tttgcctgca tcgcgcgcag cacctcgtcg atccggtgcg cggcggcgag
      841 gaagtccgcc tcggtgtacc cctttgtcgt cattgcggcg gtaccgatgc gcacgccgga
      901 ggtctgctgg gggcttctcg tttcgttggg aacgcagttt ttgttgagcg tgatgccgtg
      961 gcggtcgagc tcatcctgca ccgccttgcc ggtgagcccc gttttcgtga gatcgagcag
     1021 gaacaggtgg ttgtccgtgc cgccggtcac aacgtcgtag cccatttttc ggaactcctc
     1081 gcacatcgcg cggctgtttc tcacaacggc gtggatgtat tcgcggtatt cgtccgtgca
     1141 ggcttcctcg gcggcgacgg ccttgcccgc gatcacgtgc tcgagcggcc cgccctgcgt
     1201 gccggggaac acggcgctgt ccaccttctt cgcaagctcc ggcttacaga agatcatgcc
     1261 gccgcgcggc ccgcgcagcg ttttgtgcgt tgtggtcgtg atgatgtcgg cgagaccgaa
     1321 cggcgacggg tggtcccctg ccgcgacgag acccgcgatg tgcgccatat ccaccataaa
     1381 gtatgcgccc acgcttttgg cgatgtccgc gaagcgcgcg aaattgatga cgcggctgta
     1441 cgccgaagcg cccgcgagaa tgagcttcgg cttatattcg gcggcctttt gctccacgtc
     1501 cgcatagtcg atgaagccct gcgcgtccac gtcgtagaat ttcatatcga agagcttgcc
     1561 gctgaaattg acggacgagc cgtgcgtcag gtggccgccg ttattgagcg acatggcgag
     1621 gaccgtgtcg cccggctgca atacgctcat atacgccgcg agattcgcgt tcgcgccgct
     1681 gtgcggctgg acgttcacat ggtagtcggt atggaacacc tttttccaca tggcgcagca
     1741 gtattcctca agctcgttca cgatctcgca gccgccgtag tagcggccgc ggtttccgga
     1801 aacgcgctcg gcgggatagc cctcggcata cttgttcgtg agacacgagc cgacggcgcg
     1861 aagaacattc tcgctcacaa aattttcgct cgcgatcagc tcaacggtag cgtcctgccg
     1921 tgcctgctcg cgggcgatat aatcaaatac tttggatggc atgggtcgtc tccttttcat
     1981 ctattcattt aaaaaatgcc tccctctttg agggaggtgg attttggcga gagcaaaaga
     2041 cggagggaga gacgttttgc gcggaacgct gcgtnnnnnn nnnnattaaa aaaatgcctc
     2101 cctctttgag ggaggtggac aggttatcct gtgaagtgcg gcaccccggt gaaaaggggc
     2161 ggaggcaaaa gacggaggga aaaacgtttt gcgcggaacg ctgcgtgggc ggagcagagc
     2221 cccgccccta caggcatggt gttcgtttgc cgcaaaacca accgtccccc tccgggaggg
     2281 aagctttatc ccgcgaaccc gttcgggttt ttgctctgcc agttccagct gtcgcggcac
     2341 atatcctcga gagagtgttc cgccttccag ccgagctccc gctcggcctt gcccgggtcg
     2401 cagtacacct cggcgacgtc tccggcgcgg cgcggggcga ttttgtaggg gaccttcttc
     2461 cccgtcgcct tttcaaaggc atgtaccata tcgagcacgc tgtagccgtg gccggtgccg
     2521 agattgaaca cgaaaacacc cttccgctcc gccatatagt ccatcgccgc gacatggccg
     2581 cgcgcgagat cgacgacgtg cagatagtcg cgcacgcccg tgccgtccgg cgtgggatag
     2641 tcgttgccga atacgctcag atactcgcgc cgtccggcgg cggtctgggc gatgtacggc
     2701 atcagattgt tcgggatgcc gcgcggatcc tcgccgatga gtccgctcgg atgcgcgccg
     2761 acggggttga agtaccgcag cagcataacg gtccattcgc cgtcggcctt cgccgtatcg
     2821 gagaggatct gctcgctcat gtacttcgtc cagccgtagg gattcgtgca gccgccggtg
     2881 cgggcgctct cgtccacggg catcgtattg tccggcgaat acaccgtcgc ggacgaggag
     2941 aagaggatct tcttcacgcc gtgcgccgcc atcgtctggc agagatggat ggtcgtgccg
     3001 aggttgttgt tgtagtaccg cagcggctgc tgcaccgact cccctaccgc cttgagaccg
     3061 gcaaagtgga gcacgcagtc aaaccggtgc gtatcgaaaa gctcattgag agctgcgcgg
     3121 ttcaaaagat ccatttcgtg gtattcgagc gtctttccgg tgatgcgctc gacgcgccgc
     3181 aggcactccg cgttggcgtt gacgagattg tccaccacaa cgaccgaatg tccggcgttc
     3241 agaagctcca cgcaggcgtg gctgccgatg tatccggcgc cgccggtaac gagaacgttc
     3301 ataaatacga cctccttata gtatcacatt atctcattgt aaccgaaacc ccctgctctt
     3361 tcaagagcag ggggttccaa gacagggatt tatttttcgc tgagttcgag atcggcctcg
     3421 tccacatggt agagcgcctt tttggagagc gcgtcgtaca tgacgggcac gacgtaaagc
     3481 gtcatgagcg tggcgtacag cagtccgccg atgcacgtta cggcgagcgg ctggatgagc
     3541 gacgttgcct cgctctggcc gagcgccatg acgatgagac cgaggatggt cgtgatgctc
     3601 gtcatcaaaa tcgggcgcag acgggtgacg gcggcgtccg caatggcctc acgccgatcc
     3661 atgccgccga tgcgcagctg gttgatgtag tccacgagca cgatgccgtt attcacgatg
     3721 atgccgacga gcatgatcat gccgagcatc gagagcaggt tgatctccag ccccgcaatg
     3781 agcagcgcga ggaagccgcc ggtgaacgcg agcgggatcg tgaacatgac gatgaacggc
     3841 gatttgagat cctggaactg cgcgaccatc acgagatata cgagcaggat accgaccgcg
     3901 agcatcaaaa cgagctgtct gagcgcctcg acgatggatt cgcgctcgcc ggtgaagccg
     3961 atggtcgtgc cgtccgaggg ggagaacgat gcgagcgctt cctcggcggc gtccgtcacg
     4021 agcgtgacgt tgcagccctc ggcgatgtcg gcggtcacgt cgatgtaccg gcgctggttg
     4081 agacggcgga tgctcgaaag gctctcggtc tcctcgaccg tggcaatgtc cctgagcgcg
     4141 acggaatgca ccgtgccgtc cttcgccgtc acgtcaaacg tgagagagcg gacgtattcg
     4201 ggcgtgatct tgtctttccc ggcggtctgt acgctgacgg cataggtgtc gccgccgtcc
     4261 gaaagctcga tgagatcggc cgagccggtg agcgcctttg ccacctgcat atacacctgc
     4321 gccacggtga gctgctcctt tgccgccttt ttcttgtcca cggtgaagtg cagctcggcg
     4381 gtgggctcct cttccccggc ggcgacgttt tccgtgccgt ccacgccgcg cagcacatcg
     4441 gccacggcgt cggcggtctc gcgcagatcg tcgagatcgt tggagtatac gcgcaccgtg
     4501 atgccgctgc tcgtgagcat ctgcgtcatg gaggacatga gcgagtttgc gtcggcgttc
     4561 acggcggcct ccatatcggc gcatttctcg ttgatctctt tcgccacgcg gctcgcctta
     4621 tatccgctct cgagcaggac atagaacgaa acgtcgccct cggtggtcat catggaaagg
     4681 ctggagccgg cgctgtcgcc gatggcgccg ccgacggtct ccacgcccgg cacggtgagc
     4741 attcgcgccg ctgcctcgtc cgccgcggcg gtcgtgtcgg caaaggagct gccctccggc
     4801 atcgtgagcg ttaccatcat ctgctccatc tccatatcgg ggaggaacgt gaagcccttg
     4861 gcgatcaccg ccgcgccgct tgcgatgagc agcgccaccg cggtgaggag cgtgacggcc
     4921 ttgtggttca ccgcccagag gacggatttt ttgtatttcg gcatgaagcg cgtgaacgcc
     4981 gtttcgctct ggggcctggc gtgcgcgagc agcttgcccg cgagcgccgg gacgagcgtg
     5041 agcgctacga tgagactggc gatgagcgcg tagctgagcg tgagcaccat atcggtgaag
     5101 agctcgcgcg taaggccctc gacgaacgcg atcgggacaa agacgcagat ggtcgtgagc
     5161 gtggaggccg cgatcgcgcc gccgacctgc cgcgcgccgg acacggcgga cttgaccgtt
     5221 gattcgccga gcgcgcgcag gcggtaggtg ttttcgatca caacgacaga gttatccacc
     5281 agcatgccga ccgatatcga aagaccggag agcgacatga tgttgatgga aacgccggtg
     5341 aagtacatga gcgtgagcgc gaagagcagg ctgatcggga tcgagcagag cgtgatgacc
     5401 gtgggccgcc agtcgcgcag aaagaggaag agaatgacga ccgagaagag cgcgccccag
     5461 agaaggctcg agaggatcga gttgcgcagg agataaatgt aatcgccctg atccatgagc
     5521 gcggtgaacg tcagccccgg atactgcgcg ctcagctctt caaacgcggc gttgatgttg
     5581 tcagacacct tcgccgtcgc gtagtttgac tgcttggaga acatgaggat gagaccgttg
     5641 ttgccgttga gcgaggcata gatctccccc gcgttgtcgg cgcggacaac gtccgccaca
     5701 tcgcacacgc ggatggggtc aaggccctcg atgccgagat cgagcagcac aagggtttcg
     5761 agatcctcgc cgtccgcgat ggcgtcgccg acggtgacga tgatgccgtt gccgttttcg
     5821 tcatacacat acccggcggg catgtcgaga ttctgcgcgg caatgagact gctcagcgtt
     5881 tcgagcgtga tgacgccgtg gaggtcggcc gcgtcgagcg cggaggcgag ctgctcctca
     5941 agcgttttca ccgccgcgtc gagctgcgtc tgcgcgctgg tgagcgcggt ctgcgccgcg
     6001 gcgagctggc cgcccgcgag ggagatcttc gttacgccgc ccatgagctc gcccggcagc
     6061 tcctcgagct gcttctgcat gttttcgagc gattccatct gctttttgga ttcctcttcg
     6121 agctgggcgc agcgctcctc cgccgcggcg atctgcgtgc gcagctcctc ggcctgcacg
     6181 cgcgcggtct ccaccgcgac ggcctgcgct gcgcgcgcgg tgcgctgcgc ggagagattc
     6241 acgtcgttta agttgttcat cgtgcggacg ctgccgagat cggtgcagcc cttggcgttg
     6301 agcgcggcaa cggtgtccgc gtccagtccg gcggcctcca gcgtcgtgga ggcgtccatg
     6361 gagtcgtagg gctcgccgag cgctttggca taaagcgcgc tgtccgcttc cagcgaggcg
     6421 atgcgcgcgt cggtttcggc cagacgcgcc tcggcttccg cgagcgcggc ctccgcctgt
     6481 tcgagctggg cgttgagcgc ttcgatggcg gcgcgcgcgg aggcgagctt cgccgcggtc
     6541 tctccggcgg cggtgagcgt gtcggcgaga tcgtcctcgc cctgcttgat ggaatcggag
     6601 atgccgctcg atgcgctgag aagctggttc gcgccggcct cgaccttctc gcggttctcg
     6661 tccagctcct tctgcgcgtc ctcgagctgc tttctggcgt cttccatcgc cttgtcgatg
     6721 gcttcggcga tctttgcgtt cattttgtcg agcttggcct cgttgacctc gatggtcatc
     6781 gtccgctcga tcatgccgcc ggtcgtgatg ctcgccacgc ccgtcgtgcc ttccagcgcg
     6841 gggatgacgg tgtccttggc aaagcgagag agctcgtagc ggtccatttc ggaggaatcg
     6901 accgcggcta ccatcacggg gatcatgttc gggttgatgc ggatgatgct cggcgtgccg
     6961 atggaatcgt cccagcggcc ggtgagcatc tgcgttttct gcagaatgtc caccacggcg
     7021 ctgtccatat tcgtgctctg ctcaaactcc agcacgagca tcgaatagtt ctccgccgag
     7081 gtggaggtca cggtcttgat atcattgagc gtcgccatgc tctgctccat cggcttggtg
     7141 acgacggctt cgacctcctc cggcgcggcg cccggatacg tcgtcaccac gacgacgtag
     7201 gggtagtcca tgttcggcat caggtcgggg gtcatcttga catacgacat cacgccgaac
     7261 accagaatgg ctaccaccgc tacgaacacg gtaaacggct tttttacgct gaaggaagtc
     7321 atcgggcagg atcctttcgg gaatcgaatt cattctttcc atgatagcac cgcgccgccg
     7381 cgaaatcgtg aattttcatt gaattttaac gaaaaaacaa gacgcagccg gatcttggct
     7441 gcgtcttgac agggccttta tttgcgggag gggcagagcc cctcccctgc ggggtttttc
     7501 aaggggtatc gcaggggtgg ggcgctgctt tgcccggatg tttcatgcgg ctttgagcgt
     7561 cttcttcgcc cggaggatct gcatgacgac gatcgcgccg acaacgacga tgccgatcac
     7621 gtccgtcacc gtgccggggt acacaaggca cagtccgccg gcggcggaga ggatcctcag
     7681 ataccagggg atgctcccgc ggtagtagcc ctcgagcgcc atcccgagac cgaacatacc
     7741 gacgagcgac gtggcggcga ttatgatgac ctccacgacg gtcgtatcga cgagcaccat
     7801 tgcggggttg taggcgagaa tatacgggat gatgaacgct acgatagcga gctttgttgc
     7861 gttgaaggcg gtcttcatcg gatttgcgcc cgcaatggcg gcgccggcgt aggccgccag
     7921 cgggcgcgcc ccgccgtcga gcttgatcag cccgg
//
LOCUS       DAWAAE010000094         7864 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4102_length_7864_cov_3.409143, whole genome shotgun sequence.
ACCESSION   DAWAAE010000094 DAWAAE010000000
VERSION     DAWAAE010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_7864_cov_3.409143"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1976..1985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3955..3964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5811..5842
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1544 a         2509 c         2310 g         1449 t
ORIGIN      
        1 aggacgaggc gaaaaagctc ggcaaggtct gcttcctcgc acaggggacg atctacccgg
       61 acgtcgtcga gtccggcacg ggctcggcgg tcatcaagag ccaccacaac gtcggcgggc
      121 ttccgaagga tctcggcttt tccggcgtcg tggagccgct gcgctatctc ttcaaggacg
      181 aggtgcgcaa gctcggcctt ctcctcggtc tgcccgaaag tctcgtaaac cgccagccgt
      241 tccccggccc cggtctcagc gtgcgcgtgc tcggcgagat cacgcgcgaa aaggacgcca
      301 tcgtccgcga ggagatcggc aagtgcggct gccgcgccga tcagtatttt gcggtgctca
      361 ccggcgtgaa gagcgtcggc gtcatgggcg acggacgcac gtatgactat actgtcgcgg
      421 tgcgctgcgt ggaaacgagc gactttatga cctgcgtgtt cgcgcccctg ccgtgggatg
      481 tgctgcggcg catctcctcg cgcattacga acgaagtgcg cggcatcaac cgcgttgtct
      541 acgacatcac ctccaagccg cccgcgacca tcgaatggga gtgaccgttt cgccgtgaac
      601 gcatggaagc ggctggacga gcgcctttcc gcgggaaagg gacgcacgct ctggcagttt
      661 gtgaaattca atcttgtaag tctcacggtg acgatcttgc agcttctgct cgccaatctc
      721 ctgccgctcg cgttcgaccg tgttgtctcg ccgctgccgc cgctgctgcg cggcatattc
      781 cgtgccgacg cgcttttccc ggacggctcg aagtatgtgg tgaacggcgt tgtcacatgg
      841 ggatatgtgc tgccgttcct gctggcaaac ggtcttgcaa acatctacgg ctattttatc
      901 aacatgaaaa caacgtttcg cggcgagggt acccgcgcgg ggttcgcggt gtatcttgcg
      961 atcctcttcg cgctcattct cttcgccacg tggatgcagg gcgcgatcgt cgcagcgatg
     1021 gcgtcgagcc gcatcgctcc cctctcgcgc acgctcgcct cgttcggcgc gggcatcgtg
     1081 cagacggcgg tgctcttccc gctcgaaaaa cttgtactgt tccggcagag gccggacgac
     1141 ggaaaggaaa tgaaatgaac gaaaagctct ttgattatat tgccgtatgt cccacggcgt
     1201 tccacacctg cgcccacagc gcggacatgc tgcgcgaatc cggatatacc gagctttgcg
     1261 aggcgcagcg ctgggagatc gttcccggcg gcaagtattt tgtcacgcgg aatggttctt
     1321 cgctgatcgc gtttcgcgtc cccgccgggg agatcgcggg ctttatgatg accgccgcgc
     1381 acggcgacag cccggcgttc aagatcaagg agaacgcgga gctttcggac gggaattatc
     1441 tccgtctctc cgtggagaaa tacggcggga tgctctgcgc cccgtggctc gaccggccgc
     1501 tttccgtggc gggccgtgtt ctcgtatccg agggcgataa gctctcggta aagctcgtgg
     1561 atctccgcga gccctgcgcc atcatcccga acgtcgccat ccacatgaac cgcaacgcca
     1621 acgacggcat gagctataat gccgccgtcg atatgctgcc cgtatggatg agcagcgaaa
     1681 agggcagctt ccgcgccgcc gtcgccaagg ccgccggtgt tgcggaggac gccatcgtca
     1741 ccggcgatct tttcctttac gatccccaga agggcgtcac gctcggcgaa tacatctccg
     1801 cgccgcggct ggacgatctc cagtgcgctt tctcctcgct gacggcgttt ctcgccgccg
     1861 gggagagccg ctccattccc gtctgctgca tcttcgataa cgaagaggtc ggcagcgaga
     1921 cgaagcaggg cgcggcatcc actttccttt atgacacgct cacgcggctg tgcgcnnnnn
     1981 nnnnngcagc gaataccgcc gtctcgtggc gggcagcttt ctcgtctcct gcgacaacgc
     2041 gcacgccgtc caccccaacc acggcgaatt tgccgaccgc aaccacaccg cgcgcatgaa
     2101 cggcggcgtt gtcatcaaat acaacgccaa ccagcgctac acctccgacg ccgtttccgc
     2161 cggcatcttc cgccgcatct gcgagagcgc cggcgtgccg gtgcagtatt acgccaaccg
     2221 cgccgacatg ccgggcggct cgacgctcgg caacatctcc aatacgcagg tttcgctcaa
     2281 cgccgtggac atcggtctcg cgcagctggc gatgcactcg cccttcgaga ccgcgggcgc
     2341 ggcggacacc gcctcgctcg tcaaagctct caccgccttt tttgaacatt ccctcgttgc
     2401 cgaacgcgac ggggtctacc gctttctctg aggctcagcc gcctcccggg gaagggaggc
     2461 tcacctcccc tccccaaatt tttttgtgta tattcatcct ttgggagtgt gaaagatatg
     2521 acaaagtaca ttttcgtcac cggcggcgtc gtttccggcc tcggcaaggg catcaccgcc
     2581 gcctcgctcg gacgtctttt gaaggcgcgc gggttcaagg tcgcggcgca gaagctcgac
     2641 ccctacatca acgtcgatcc cggcacgatg agcccttacc agcacggcga ggtctatgtc
     2701 accgaggacg gcgcggagac cgatctcgat ctcggccact acgaacgctt cattgacgag
     2761 gacctcaaca agttctcgaa tctcaccacg ggcaaggtgt attcccgtgt gctcgaaaag
     2821 gagcgccgcg gcgagtatct tggcagcacc gtgcaggtca tcccgcacgt caccaacgag
     2881 atcaaggaat tcatctatca ggtcggcaaa aagtccggcg cggacatcgt catcaccgag
     2941 atcggcggca cgaccggcga tatcgagagc cagccgtttc tcgaggccat ccggcaggtc
     3001 gggctggagg tcggacgtga gaatacgctc tatatccacg tgacgctcgt gccgtatctc
     3061 aaggcctcgg gcgagcacaa gtccaagccg acgcagcact ccgtcaagga gctgcagggc
     3121 atgggcattt ccccggacat catcgtgttg cgctgcgacg agccgattga ggacgagaac
     3181 atcttccgca agatcgcaaa cttctgcaat gtggagagcg actgcgtcat cgagaacatg
     3241 acgatcccgg tgctgtatga agcgccgctc atgcttgaaa aggcgcacat gggcgacacc
     3301 gtctgccgca agctcggtct cggccagccg aaggcggatc ttgccgagtg ggaggaaatg
     3361 gtcagccgca tccacggccg cacgaagcgc gtgcccatcg cgctcgtcgg caaatatacg
     3421 cagctgcatg acgcctatct ctccgtgatc gaggcgctgc gccacgcggg ctacaccgtg
     3481 ggaacgcacg ttgccatccg ctgggtagac tccgaaacgc tcaccgaaga gaatctggaa
     3541 gaaaagctcg gcggcatgtg cggcattctc gtgccgggcg gtttcggcga ccgcggcatc
     3601 gagggcatga tcctcgcggc gaagtacgcc cgcgaaaaga acatcccgta tctgggactg
     3661 tgtctcggca tgcagatcgc cgtcatcgaa aacgcgcgca acgccgcgca tcttgaaggc
     3721 gcgaactccc gcgagtttga cgagcgctcc ccctaccgcg tcatcgactt catgcccggc
     3781 cagagcgacg agatcgccaa gggcggcacg ctccgtctcg gcgcgtatcc gtgcgacgtc
     3841 atgcccggat cgcttctgga aaaatgctac ggcgcgcagc acatctccga gcgtcaccgc
     3901 caccgctacg aattcaacaa cgaataccgc gaggctctca ccgccgcggg gctcnnnnnn
     3961 nnnnttctac atcggcgtgc agtatcaccc agaattcaaa tcccgtccca accgtccgca
     4021 cccgctcttc cgcggctttg tcgccgcggc gctcgagctt ttcgagccgc gcggataagg
     4081 cccgtccgca tgggaaaaat tattgacgct caccggcgga aagtggtatt ataaagtgtc
     4141 agtttattgc ctactctctc tgattttgga aaggactctt ttatgggaaa atatttcggc
     4201 accgacggtt tccgcggcaa ggccggcgag gtattgaccg ccgagcacgc cttcaggatc
     4261 ggccgttttc tcggctggta ttacgcgaac cgtcatgcga gcggcacggc ccgcatcgtc
     4321 atcggcaagg atacgcgccg ctcctcctac atgtatgaga gcgcgctcgc cgcgggcatc
     4381 acgtcctccg gcgcggacgc ctatcttctg catgttacaa cgacgccctg cgtcagcttc
     4441 atcacgcgca ccgaaaactt tgactgcggc gtgatgatct ccgcgagcca caatccctac
     4501 tgggacaacg gcatcaagct catgaacggc gtcggcgaga agatggacga cgcgctgcag
     4561 gacgagctcg aagcttacat cgacggcgat cccaacagca ttccctgggc agtggaggac
     4621 aagatcggcc gcacgatcga cttttactcc ggccgcaacc gctacatcgg ctatctcacg
     4681 agcatcgcca cccgctcgtt cggcgaccgc aaggtagcgc tcgactgctc gaacggcagc
     4741 gcgtggatga tcggccgcgc cgtattcgac gcgctcggcg ccaagacgta cgtcatcaat
     4801 gacaagcccg acggcgtgaa cgtgaacctg cgctgcggct ccacccacat tgagggattg
     4861 cagcagtacg tccgcgaaaa caagctcgat gtcggctttg cctttgacgg cgacgccgac
     4921 cgctgtctcg ctgtggacga gctcggcaat gtggtgaacg gcgatcagat catgtacatc
     4981 tgcgccaagc acatgaaggc caacggccag ctgccgagca ataccgtcgt cacgacgatc
     5041 atgagcaact tcggtcttta caaggcgttc gaccgcatcg gcatcgccta cgaaaagacc
     5101 gccgtgggcg accggtacgt ttacgagaac atgaaggcca acgatcatat gcttggcggc
     5161 gagcagtccg gccacatcat tttccgcaag tttgcccata ccggcgacgg cctcatcacc
     5221 gcgatcatgc tcatgggcgt gctcatcgac acgcagctcc cgctctcggt gctggccgcg
     5281 gaagtgaaga tgtacccgca ggtgctcaaa aacgtgaagg tggacgacaa ggacggcacg
     5341 ctcgcggacg agaccgtgaa ggccgccgtg gagaagtgca ccgccgcgct cggcgacggc
     5401 gggcgcgtgc tgctgcgcaa gagcggcacg gagccggtgc tgcgcgtcat ggccgaggcc
     5461 gggacgaccg agctctgcga gcagatggtg gacgccatca tcgccgcgat ggatacgtcc
     5521 ggccatctca tcgaggtgaa aaagtaatga agctcacgat cccggatctt tacgatcttg
     5581 accatacgct tgccggagac tatctgcggc agttcaccta tccctgggag gcgctcgccg
     5641 gcatcggcga tatgatctcc gcgctcggcg cggcgctccg cccggacgaa tacgaccatc
     5701 ccgaggaggg cgtttgggtc gcgcgcgacg cgaaggtcta tcccaccgcg tatctcggcg
     5761 cgccgtgcat catcgggcat aaaaccgagg tgcgccccgg cgctttcatc nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnttataatt atgtcggcga ctccattctg ggctacaagt
     5881 cccacatggg cgcgggctcc atcacgagca acgtaaagag cgacaagctc cccgtcgtta
     5941 ttcacaacga aggcgaggag atcgagaccg gccgcaaaaa ggtcggcgcg atgctcggcg
     6001 actatgtgga ggtcgggtgc aacagcgttc tcaaccccgg caccgtgatc gggcgggacg
     6061 cgagcgttta ccccacgtcc tgcgtgcgcg gcgttgtgcc ggagcggtgt atttacaaag
     6121 ccaagggcaa tattgccgta aaaaaatgat aaaggagaag atgtcttgaa agtaatcgtc
     6181 gccgaaaatc acgaggaggt cagccgtctt gccgctgaca ttctggagga aacggtccgc
     6241 acgaacccct gctgcacgct cggtctcgcc accggctcaa gcccggtcgg catgtaccgg
     6301 gagctttcgc ggcgctgccg cgaggagggt ttggatttct cccgcgttca cagcgtgaat
     6361 ctcgacgagt atgtcggtct cgcccccact catgagcaga gctaccgtta tttcatggac
     6421 agcaatcttt ttgaccacat caacatcgac aaagccaaca cctatgtcgc caagggcacc
     6481 ggcgacgcgg aggaaaatct ccgcgaattc aacgccgtgc tcgaccgtac cgacattgac
     6541 gtgcaggtgc tcggcgtcgg cccggacggt catctcggct tcaacgagcc gggcgacacg
     6601 ctctgcgaca gcgcgcacaa ggaggtgctc gacgagagca ccatcgacgc caaccagcgc
     6661 ttcttcgcct cgcgcgacga tgtgccgcgc tacgcgctca ccatgggcat gggcaacatc
     6721 ctgcgcgcga aaaagctcct tatgatcgta tccggccaca aggaggacgc aatgcgccgt
     6781 ctgctgctct ccgagcgcat cgacccgcac tgcccggcaa cgtttctgcg tctgcaccgc
     6841 gacgccgtgg tgctgctcga acgctctctc gccgacacga tcggctataa ggcgtaagcc
     6901 tttcatccac cgctcccctg cggatacccc gcaggggacg ccccgtttac tttggaggat
     6961 attcttttat gtgtggaatt gtaggattta ccggctcgca ggcggcggct ccgctgcttc
     7021 tggacgggct gcgcaagctg gaataccgcg ggtatgacag cgccggcatc gccgtgcaga
     7081 aggacggcgc gctgcatatg atcaaggcgt ccggccacat cgacgcgctc gcggagaaaa
     7141 cacaaaacgg cgcgctgctg cccggcactt ccggcatcgg ccacacccgc tgggcgacgc
     7201 acggcgcgcc caccgacacg aacgcgcacc cgcatatgtc cgccgacggc aaatttgcca
     7261 tcgtccacaa cggcattatc gaaaactacg cggcgctgcg cgacgagctt aaaagcaagg
     7321 gcttcgtatt ccggagcgag accgacacgg aagtcatcgt gcatctgctc gacatgtact
     7381 ataccggcga tctcaaggcc gccgtgctgc acaccgccgc acggctcgag ggctcctatg
     7441 cgctcggcgt tctctgcgcc gaccagccgg ggacgatctg cgccgtgaaa atggcatcgc
     7501 cgctcattct cggcgtgggc gtcggcgaaa atttctttgc ctccgacgtg accgcgctcg
     7561 tgagccacac caaaaacgtg atctatctgg aggacggcga gtttgctgag ataacgcccg
     7621 actccatttc catttatgac agcaccggcg cgacggtgca ccgcagcatt tcccgcatcg
     7681 tctgggatat cgaagccgcg gaaaagggcg gctatgagca ctttatgctc aaggagatca
     7741 tggagcagcc ccgcgcgctc aaggcgacca tcgccccgcg catcaagaac gggaagatcg
     7801 tcctcgacga ttttgacgag gcgttccgcg ccgacttccg gtacatcaac cgcatcgtca
     7861 tcac
//
LOCUS       DAWAAE010000095         7807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4133_length_7807_cov_2.943885, whole genome shotgun sequence.
ACCESSION   DAWAAE010000095 DAWAAE010000000
VERSION     DAWAAE010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_7807_cov_2.943885"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1564..1573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7348..7357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1498 a         2469 c         2381 g         1439 t
ORIGIN      
        1 gccgcgcctc cgcctccgaa aacgagccgc tctcctcccc tgcggtgaca agcgaggcga
       61 tcttcttttc aagctccgcg ccctgctgca cgccgagccc cgccgggccg atgaatgccg
      121 tgcgctcaaa tactggcagt gacgcgccgg tgagcgtttc tccgacgttt tccccggtaa
      181 gatcgagaac cggctgaccg gagcgggaat acacagcgga aaagacacgc atgggcgccg
      241 agggaatgcg cgtcgagcgc gaaagagtaa tgtccttccc gccgaacgtg atctccatcg
      301 tccccgccat cggggaaccg ctccacggag cgtacagcac cttgtccggc tttttcccgg
      361 cgcggccgcg ctgggcggtg tcgatgccgt agagcatggc gcgcagaaac gcgcaccacg
      421 ttgtttttcc gtattcgttg ggcgcttcaa tgatattcag ccccggtgac agctcaagcg
      481 tgcggttttg cagcacgccg aagctcgccg tcattttccg cacgatcaca gccgcacctc
      541 ccaaccttcg agcgcggcaa tgccgcagcg cacggcgcgc aggagcattt tcttttcctc
      601 ctcggtctcc gcggtctcga tacgccgctg catccggcgc agaaaccgtc cggcgaggct
      661 gtcctccccg gcgcgccgcc agagatcgcg cggcggtacc gtttcgtccg agaacgtgag
      721 cgcgaagaaa tcccgttcca gcgcctgccg gagataggca agatccggcg cggcaaaact
      781 ctcgccggtc aggatgatct tatatacatc gtccgccgtg tcttttggca gcgcggcgcg
      841 gatcgcggcg agagcatccg ctcccggctc cacgcgcagc ttttcgtacc ggcgcgcagc
      901 ggtcgggatg aaaccggctt tcaccgtttc gccgtcaata tcgatctgca aaattccctt
      961 ttcgccgcac tcgtcaaagc cgcgcccctc cggacagccc ggccaggcat aaaagcagct
     1021 cccggcacgg cgcaggccgc tccccagatg gatatgaccg agcgcggcat atcgcatgcc
     1081 gctttgcgag agctcctcct ccgtcaccgg gcagtacggc gagccggcgc ggcccagctc
     1141 ggcatgcagc accagaagct gcgttacgcc ctcctccgcc ggagcatgaa agccgcgcag
     1201 aagcgcctcg cacgagggcg acgtataccc cgcgccccag acgcgcgcgc cgagctctgg
     1261 caggaaaacg ctctccagac ggggcgtgcg gaagatatgg acgttatccg ggaagggcag
     1321 gagcgcatac ggcgagcgcc gggagtacca gtcatggttt cccggcgcaa taaatacggg
     1381 gacgcgcacc tttccgagcg cggcaagcag cgcctcgccg gtctcggcgt aggcgctgcc
     1441 gctgtccaga agatctccgg caaggaacag gagatcggca tttcgctggt ttacctccgc
     1501 cgcgatgcgc tccaaaagcg ctctctgctc gctgcggcgg acggacgcct tttccgcgtc
     1561 aagnnnnnnn nnncgattca aagggggaat cgagatgcag atcggcagca tggatgatcc
     1621 ggatcatggc gttatgcgga ttgcttcgac cgcggtgcat gcgccggttt cggtatcgac
     1681 ggtgaaaacc gcgccctcga gctttccggg gcccttcgcc tgctcgtacc ggcgcggggg
     1741 atcgccgagg aatttgcgga tggactgctc cggcgcgatg ccgagcaccg attcgctcgg
     1801 ccccgtcata ccgagatcgg aaatgtatcc cgtgccgcgc ggtaaaaccc gcgcgtccga
     1861 ggtctggaca tgcgtatgcg tgccccacac ggcggagacg cgcccatcga gaaaccagcc
     1921 catggcgagc ttttcgctcg tcgcctcggc gtgcatatca acgagcacca tgcgcgtctg
     1981 attttctgcg agcagcgcat ccaccgtcgg gaacgggtta tcggtgttcg tatcgagaga
     2041 aaaccgcccc agaagattga tgacgcggat ggggccgaac ggcgttgtat acgtcgcgtc
     2101 gccccggccg gggcactgcg gggcgtaatt ggccgggcgc aggatgcgcg ccttctcgtc
     2161 gagatacggc tgcagttccc agcgcgtcca tgtgtggttg ccgagcgtca caacgtccgc
     2221 tccggcggcg agaatggcgt ccgcctgcgc gggcgtgatg ccgacgacgt tggcgttttc
     2281 cccgttcaca acggtgaagg aaacgccctt gagcttctgg agcggcttta aatgctcctt
     2341 gagaaaggcg aggccggtct gcccgacaac gtcgccaatg gccaaaatat tgatcgtcat
     2401 gcgtttttct ctccgtccgt tccggattcg ttcgtcccgg cttcgcctgt ttccgcttcg
     2461 ccggcacccg tttcgtcccc gtcgttcagg tcatccccca cggagttgaa gatatggaaa
     2521 ataccgctcg agttgagatc catgataatg gcggcggtga tcggcaggcc aaaaagaccg
     2581 ataagaccgc cgaagagctt ggaaccggcg tacatgcacg tgagcgttac gattgggcgc
     2641 agaccgacgt gcttgccgac gatcttcggc tcaatgatct ggcgcgcgat tacaacgacg
     2701 gcatagatga tgaggatgcc gagaccgcgc atcgtgttgc cggtgagaag cgtgatgatc
     2761 gaccacggaa ggaggatcat gccgctgccg acgatgggca gcgcatcaaa aatgccgatg
     2821 aggataccga aaagcagcgg gttccccacg ccgacgatca gaaggccgac ggtgatctcc
     2881 gccacggtga tgcagaagat gatggcatag gacttgatga actgccagat catctcaagg
     2941 aacgagttct tgacattcac cgcaacgcgc agcggttttt cgggcaggtt tttccgaatg
     3001 aagagcctga tgcggtcaaa gtccacggaa ataaagaccg tggcgatcag gcagatgatg
     3061 ctgttgagca atatgctcgg caggcggctc gcaatgccgg tgacgccgct gacgatcttg
     3121 ccggagaggt tggtgaccgc gctgccgaga ttggagatca tgccgggcag ggcctgctca
     3181 atagcgtcca tgatggacac atcgaaccgt tcgcctacct ccagcaccca gttatagagc
     3241 tttgtgatgc cggggaggac gctctgttca taaaacgtcg gcgcgaacga cacgaaatcc
     3301 acgatcgtat cggtcaggcg gacgacgatg agcgtcagaa ggccgccgac gacgatgaac
     3361 agcacaagca cgctgatgat gccggccaca ttgcggttca tatggaattt ccgcttcacg
     3421 aggaccttgt tcccgtcctt gccgcggaca tagcgcgttt cccggctcag cgcgcgggag
     3481 atcggacgca cgaccgccgc gaaaatgaac gcgaggaaga tcggcatcat tatgttgatg
     3541 aggtacttga acaccaggaa gatgatcgca acgatcaccg cccagtacgc aatgttgata
     3601 aggaagttta cgcggggatt cgtttcccgt ctttcttcca taaaattccc tcctttcagg
     3661 gtatcacata tcggctgtaa ggaacggcag cgcgagacgc tgctcctcgc cgtcgagcgt
     3721 atattcaatg cgcggcatgc cgtccatgcg tacacgcacg ttttccgcgt ccagcagccg
     3781 gcgcagctcg cggtaggtct cgcgcttttc ctcgtcatag gcgttcatgt cgtcggaggt
     3841 gaagaacacg ctcccgagca gcgcgtcggc gcgcgcaagc atcgttttct gcccgccgga
     3901 gagcttgaga ttgtcgctgc ggaggaagaa cacatccgga tcgttcccga aagcgcggcc
     3961 gttcaaaccg cggcggaaga gcgtattttc aagcgaatgc tttgtggaga tgcgctcgcg
     4021 gtgcgccatt ttcattatga acgagccgtc ccagtcgagc cccacatcgc acccgatgcg
     4081 gcagtagtcc acgagtccga acgcaggcat gagcggcgcg ccgcagccga tgatgagatg
     4141 ctcgccgcac atctcgcgca ggaaacccat cgcgcggcgc atgcgctttg cgcggctctc
     4201 cgtcgggaga ttgaccggcg cggcggcata gaggaaatcg agcttcacaa gatcgaagcc
     4261 ccagtcggtg aacacgcggc ggaacacgtc gcgcaggtac tgcgcggcct gcggatggtc
     4321 aaagtcgatc gcatagaagc cgccccagtt catgccgcag taccacggct tgccgtctac
     4381 acgcagcagc cagtccttgt gccgccggaa gatctccgaa tccttctggc agacgaacgg
     4441 cgagagccag agacccgcgc gaaagccatt ttcatggatc ttgtccgccg tgccgcgcaa
     4501 tcccttcggg aatttcttcg cgtccgtcgt cagccagtcg cccacggcgg gctcccagcc
     4561 gtcgtcgatc tggaatacat cgccctcgga gagaaccccg gcacagccgg agagatcgga
     4621 gaggatgcat ttctcgtcga tatcctgata gcggttgtac cagctcgaat acccggcaat
     4681 gcgctctgcg ggctttttcg gcagacccat ggcggcaaac cagccgtcgt agacctcctt
     4741 ctccgcgccc tcggcataga agagatcaaa aacatggaac tctccgttgc agcgcacgcc
     4801 gcagcagtcg cgcgagagcg tgagctttcc ggcattggcg tcatagcgga tcatggtgta
     4861 gccgctcgat tcatcgagcg agccgagcag ccggaaatgc tcgccgcgcc gccagtaggc
     4921 ataggattcg ccgtgcgtca cgcccggcgt ttccgggtaa tcggtgaaat agtaatcgcc
     4981 gtagcgctcc agtcccagcg gcttgaagcg cgccagcggg ctcgcgaagg agggcgtgcg
     5041 gtccttcaca ccgcactccg gcgagtacgt ccagctctga aagccgttga tgaaaatgcg
     5101 ctcgcccggc tccgtatccc agagcatcgc gccggtcacg cttttaagcg tatcgtccgg
     5161 cagctcacag acgatgcggt tctctcccgg ctgcatcagc gtgctgccgg tgaccgtcgc
     5221 tccgttttcc agttccacgc gccataagag ttccatattt ctcactcctg ttcgacgttt
     5281 tgtccttgta tcgagaagcg cacctctttc tccggcaaac cggcggcgga aatgcggaga
     5341 acggcctccc cgctctcgcc cgtggtgcgc acataggtac cggtcatgcc tccctcggca
     5401 gtcacggtgt cgggcccgat gagcgccgcc gcgcccgtaa gcgagaaccg caccgggagc
     5461 tgcgcatagg gggccggggt gccgtgctca tcgagaatgc ggacgcgcac cgcggcggcg
     5521 tcccaggtat ccccttcgca aagcgccgtt ttgctcacgc gcacctccat gtgcagcgcc
     5581 gcgcccgagg cgcgcgtcac gctcgcaacg gtcttgccgc cggtacaggc atcaaagcgc
     5641 cagcgcgtcg ccgcgccgcc ccagttgccg acatactttc cgaagagcgc cacgccgtcg
     5701 tccatcgtga gcttgtagcg cgccatggcc cacgccatgc gcgccttgtc cgcgaccggc
     5761 atcccggcca gaccgttctt tccggcggaa acaaggcatt ttcgcagcag ctccgccttg
     5821 tcatgaggga aatgctcctg cgtttcgagc agctcgccga tcgtatcgtc gatcgccaca
     5881 gggccgtgcg gcagaccgtc ccagcccttt gtgcggaacg aggcgacgaa ccggtcgttc
     5941 ttataaagcg cgacctcctc ggcgttcgta aacgcccaga tatcaccgat gtttcccgcg
     6001 ggataatcgc cgatatccat cggcgagccg atctcgagca ccggcgtctt gtccccctgc
     6061 gaggcataga gcgccgcggc gagcttcggg ttgcggaagg cgtccatcac gccgtggtag
     6121 cacacgcggt cgcccgagcc gaagtccttg tgcgtcggat aatcaaacat cacccagccg
     6181 aagcagccgg aaatctcgcc gtcggacgcg gcggcattct gcacgcgcgc atggcgcagc
     6241 gcgtgctcct gccgcttttc ccacgggtcg aaggacttcg tcgggaacat gtggccgttg
     6301 cactcggaga taagataagg cttgtccata tccggcgtca cggcgctgcg cttctttgcg
     6361 ccgggtgccg tgccgtcgtg cgaaaagtcg ttataggcgt atacgtcctc caaaagatgg
     6421 ctcttttcaa gatagcgcac gccggaggtc tgacgggatg gatcgagcga atgtgccagg
     6481 gcattcgtct cgcggtagaa gtcatcgtca tccagacttt cgttgatgcg aacgccccag
     6541 aggacgatgg acggatgatt gcggtactgc gtcaccatct cgcgcacatt ctccaccgac
     6601 cggcgcttcc acgccgcgtc gccgatgtgc tgccagccgg gaagctcggt gaacacgagc
     6661 agccccagct cgtcgcacgc atcgataaag tgccggctct gcggatagtg cgacgtgcgc
     6721 accgccgtgc agcagagctc ttccttcaat atccgcgcgt cctcgcgctg gagcgcctcg
     6781 ggcgcggcgt agcccatgta gggatagcat tggtgacggt tcaggccgcg gaggaacacg
     6841 cgctcgccgt tgagataaaa accgctgcgg cggaattccg ccgtgcgaaa gccgaagcgg
     6901 accgtctttt cgtcggtcac ggcgccgctc gcgtcaagca gctcggcgcg gatcgagtag
     6961 agattcggct cgtccagcgt ccacgggcgg gcgtttgagt agtttacata atatttatta
     7021 tcgtcaccct ccgtcatggc gcagaccgcg cccgcagcgt cgagcagcgt gacgcgccgg
     7081 cgggcgccgc tgccggagac ggtaagggcg atctcggcgc gcgtaagcgt tggcgttgta
     7141 acgaacacgt cctcgatcac gtccgcgccg cggacatcga gccatacctc gcggtacagc
     7201 ccgccgtaag cgagatagtc cagcacgaag ccgaacggcg gcacggcggg attttcggtc
     7261 gtgtcgagcc gcacggcgag gcggttttcc atgccgtagc gcacggcgga cgtgatctcc
     7321 acgcgaaagg ccgtgtagcc gcaggcannn nnnnnnnggc atgctcggcg agcttctcgc
     7381 cgttcaaata tacctcggca aaatgcgccg cgccgtcaaa ctgcagaaag agtcgcttgc
     7441 cgcggtactc gagcggcacg gagaggcccc gccggtaacc gcagatgccg gcatagtcct
     7501 ccggctttgc atagtgcagc ggcacttccc ctgccgtgtg cgggagatcg acaagcggaa
     7561 agcgtccctc gccgtgggca aagccggcgc tccactcgcg ggtgaattcc caatcgctgc
     7621 aaaggctgat catggcgtcc ctccggaaag aaattactct tttcatttta catggaaagc
     7681 ggcgttattt caaccgaaag aaaatattta acaaaatcac agcggcgtcc cgaacggcac
     7741 gccgctgtga tttcactgca aattatcctc cacaggcctc ccccggatgg gggaggcttg
     7801 cggggat
//
LOCUS       DAWAAE010000096         7793 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4144_length_7793_cov_2.926079, whole genome shotgun sequence.
ACCESSION   DAWAAE010000096 DAWAAE010000000
VERSION     DAWAAE010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_7793_cov_2.926079"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3425..3434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1601 a         2405 c         2210 g         1557 t
ORIGIN      
        1 gttcctgctc atcaccgtgc agatcaacga gtttgccgaa aaaggctcct acagccttgc
       61 ctgcgccttt gcgacgatcc tcatcgtcat cacctacggc agcgtgctgc tgatgaatct
      121 ggtaacaaag cacttcggca ccagcagaaa aatcaaggag ggaaagtaag ccatggaaaa
      181 agtgaaaaaa ggcgtgcgtc tcgaacatat nnnnnnnnnn tttttacgcc gtcaaggata
      241 actccctcgt catcgagccg ggcgaattcg tcacgctcct cggcccctcc ggctgcggca
      301 aaacgacgac gctgcgcatg atcgccggct ttgaaagccc ggacgagggc gagatctatc
      361 tcggcgatga ggctatcaac gctctcacgc cgaataagcg cgacaccgcc atggtgttcc
      421 agagctacgc ccttcttccc cactacaacg tgtttgataa cgttgcctac ggtctcaagc
      481 tgcgccacgt gccgaaggaa gagatccgcg agcgcgtgat gaagatcctt gatctcgtgg
      541 agctcaccgg tatggaggcc cgcatgacca accagctctc cggcggtcag cagcagcgtg
      601 tggcgctcgc ccgcgcgctc gtcatcgagc cgagcgttct gctcttcgac gagccgctct
      661 ccaacctaga cgccaagctg cgcgtcacga tgcgcacgga gatccgccgc atccagcagg
      721 aggtcggcat caccgccatt tacgttacgc acgatcagag cgaagctatg gcgctctccg
      781 accgcatcat catcatgcgc tccggtgttg tggaacagat cggctccccg caggagatct
      841 attaccaccc cgtcaacgag ttcgtcgccg acttcatcgg cgaggcgaat ttcctcaagg
      901 gcaggctgga agcgctctcg gacaaccggg cgcagctcaa cgtttcgggc gatatctgcc
      961 acgccgctcc cgtgcccggc atggaagtcg gcagggatta tacgatcgtt ctccgtcctg
     1021 aggccgcgtc tctggccgaa gagggcgggc ttccgtgcga ggtcgttctc tcctgcttca
     1081 tgggcagcta ccagaactac catgtcaagg tcggcgatac gctcgtgaag ctgaccgacc
     1141 ccaaccccaa gaacaagcgc atttacaagg tcggcgagcg ctgccatctg gtctttgatc
     1201 cggagagcgt gcacgttctc tgatgccccc tattttgccg caccccgtcc gcccaaagcg
     1261 gacggggtgt ttttctgccg ccgcggattg gcagagccgc acggttccac gtatgctgta
     1321 aggggtgcgc ctcgaagctc tcaccgccgt caagacggcg gtttcggcaa actattcggc
     1381 gggagaccgc atcacctacg ctctctcgct cgtgaacacc ggcacagcgg ccatcacgga
     1441 ttataccgtc accgataatc tcggcggcta taccgtcggc gcaaacaccg tctacccgct
     1501 cgcctacaac gccggaacgg tgcgctatta tattaacggc gttttgcagg cagcgccgac
     1561 cgtcaccgcc gggccgccgc tcaccgtgac cggtctcacg gtccccgccg gcggcagcgc
     1621 cgtcctcatc tatgagacga ccgcaacgcc cgtcgcaccg ctcgcaacgg gcagctcgat
     1681 cacgaacacc gcgacgatca ccggcgccgg tctcacaaac ccgatcaccg cgcaggcgac
     1741 ggtggagacc gcgaacagcg ccgatctcac catcagcaag gccctctcgc cgacgaccgt
     1801 tacggaaaac ggccagatca cctacacgtt cgtcatttcc aacaccggca ataccgaagc
     1861 gacggcgacg ggcaatgttg tcgtcaccga cacgttcgcc cccattctcc gcaacatcgc
     1921 cgtcacctac aacggcacgg catggacgga gggcacaaac tacacctaca acgccgcgac
     1981 cggcgttttc acaacgctgg ccggacagat caccgttccg gcggcctcgt tcacgcagaa
     2041 cacagacggg acgttcaccg tcacgcccgg cacggccaca ctcgtcatca ccggtacggt
     2101 ctgaccattc accatacaaa cgattccgtc cccggcttcg gccggggact ttccttaccg
     2161 aaaaagcact tcccgttccg gtgtttccat ggtataatag ccgcaattta cgcctttcga
     2221 caaactgtgc acgtattgga cggagtaaga gagacttatg gaaaagaaaa ccttttatct
     2281 tacctgcgag cggacatggg tgtacgacac gctcatattc gccgctggta tcctcggctc
     2341 gtatacctac atgctctgcg gcggcatttt ctgcaacgcg caaaccggca acatcgtgct
     2401 tctcggcatc gcgctcggca cgcggagctg gacaagcgcc ctgtactatg ttatcccgat
     2461 ctccgcctac gcgctcggcg cgttcatttc cgagctcatc cctgacgatg tgaagcaccg
     2521 cttccatatc cgctgggaaa cgatcctcgt cgcggtcgag atcgtattcg tcgccgtgct
     2581 cggagcactt ccggcatcga cgccggtgca ggtctttcag gtgtccatca actttatcgc
     2641 ctccatgcaa tacaacacgt tccgcaactc cgagggcgtg ccgatggcca caacgttcgc
     2701 caccaaccac gtccgtcagc tcggcatcgg tcttgccaag gagctgcacc gccgccacgg
     2761 tgacacgtct caccgccgcg ttctcaagcg gcatttttcc atggtcctct tcttcctcgc
     2821 cggaacgatc ctcggcgcgg tagcgtgcat tttccttggc cgccgggcgg tctgggtcgc
     2881 agcgctcccc tacgccatcg ttttcgctgc ctttctccac gccgacctca cctcggaaaa
     2941 agagtggctg gagcgcgatc ccgcgggaca ctgagcagcg gcgcacgttt ctcttgcttt
     3001 ccggatcatt tcgcgctata atgcgctaaa tcgtaaatgc aggagaaata tattatgtcc
     3061 ggataccata aaaacgaagt ttacgccctt ctcaacgata agggcattcc ttacgaagcc
     3121 gttgagcacg aagcggtatt cacgatggag gagatggaac gtgccgggat cgatgcttgt
     3181 ggctgcatct gcaaaaacct ctttctgcgc gatgcaaagg gccggcagca ttatcttgtg
     3241 accgtccctg atgagcggcg ggtcgatctc aaatggctct cggcagcgct tggctcctca
     3301 aagctcagct ttgcctccac cgaacggctc gatacatacc tgaagctccc gcagggcagt
     3361 gtttctccgc ttgggatcct caacgatgag agccgctccg ttatcttcgc cgcagatgcc
     3421 gcgcnnnnnn nnnntgttaa agaatttgat gtatatcttc tcttgaaaat ccgaccgacg
     3481 cgctgtagaa tctctccata attttttacc ggaaggaagc ccggcatact gcccggcgct
     3541 tctccctttg cgggggccgg agtttcgcgg ttccgcccct ttcggcggga ccgcgctctt
     3601 ttttattctg ctgcgaaagg atgggtttgg ccatggcaaa aaagaggatc ctcggagtga
     3661 tcggtttggg acacgtaggc gcgcatgtcg cttacgcgct cgccgtacag ggtattgcgg
     3721 acgagctgat attgatcgac aaaaacgcac agaagctcgc aagcgaaata caggatctgc
     3781 gcgatgccgc agcgtatctc ccgcaccgcg tcagctttca ggccggggag ttttccgatc
     3841 tcggcgaatg cgacgtgatc gtcaactgtg ttggaaagat cgagcttctc cgcgaatcgc
     3901 acgaccgtac cacagagatg gattttacga tcccggcggt gcgcggcttt gccgaaaaaa
     3961 ttaaaaaaag cggctttaac ggcgttgtta tcaacatcac caatccctgt gatattgtaa
     4021 cgcatgagct tgctttgggg cttgggcttc cgagagggcg cgtattcggc accggcaccg
     4081 ggctggacac cgcgcggctt ctcagcgcgc tggcccggca gaccggcatc gaccacaagt
     4141 cgatcacgtg ctacatgctc ggcgagcacg gaaataagca atttgcccca tggtcgtgcg
     4201 tcagcttccg cggcatgccg ctggacgtct gggcaaaaac ggacgaacgg ttccggtttg
     4261 accgcgaggc gctgcaaaaa gaatccatcg gcggcggatg ggtcacattc tccggaaaat
     4321 actgcaccga atacggcatt gcgacgaccg ccgcgcgcat ggcgcacatc gttctgcacg
     4381 acgaaaaggc cattatgcct gccagcgcgg agctttgcgg agaatacggc gaatccggac
     4441 tgtttgccgg agtcccctgc gtgctcggcg caaacggcgt ggagcagatc gtcgagctgc
     4501 cgctcacggc agaagagcag gcgcagttcc acgcctgctg cgacggcatc cgcgccaaca
     4561 tggagcattg gaaagacatc cgcaatatct gaaaagagga gcttcattat gaacattaca
     4621 atcactaaaa aagagaaccc cggaaagctg ccggaaacgg atgagctcgg cttcggaaaa
     4681 tattttaccg atcatatgtt tctcatggac tattccgata ccgacggctg gcacaatgcg
     4741 cgcatcgtcc ccttcgggcc ggtcagcatg tcgcccgccg cgggcgtgct gcactacggc
     4801 gctgaggtgt ttgaggggct caaggcctac cgccgtccgg acggcggcgt gcagcttttc
     4861 cgcccttggg aaaatatggc gcgtctgaac cgctcgtgcg accgtatcgg tctgccgcgg
     4921 atcaatgagg aggacgggct gcaggcgatc cgggaactgg tgcgtgtaga tgagcgctgg
     4981 gttccctctg agccgggtac gtcgctctat atccgtccgt tcctgtattc cacgaatccg
     5041 acactctctc tgcacggcgt ctattcggcg acgtttgccg tcattctctc cccttccggg
     5101 aactatttca aaaatgggct gaagcccgtc cccatcatgg tggagaccga agacgtccgt
     5161 gccgttcgcg gcggcaccgg tgaggcgaaa tgcggcggga actacgccgc tgccaaccgc
     5221 gccgcggaca aagccgccga aaaaggctat gcgcaggttt tgtggctcga cggcgtcgag
     5281 cggaaatatg tggaagaggg cggcggtatg aacgtgatgt tcaagatcaa cggcaccgtt
     5341 gtaacgccga tgctcacggg ctccattctt cccggcatca cgcgcaaatc ctgcatcgag
     5401 ctgctgcgga gtcagggtat tcccgtggag gagcggctca tcagcgtgga cgagctcttc
     5461 gcggccgcga aagccggcac gctggaggaa gcatggtgcg tcggcaccgc ggcggtcatc
     5521 tccccgatcg gtgagcttgc atggaacggg gaaaaatacg agatcaacgg caacaagatc
     5581 ggtccgctct cgcagatgct ctacaacgag ctgaccggca tccagagagg caccaggccc
     5641 gatcccttcg gctgggtcga ccgggtctgc tgagccgaat ataatatcaa aagctccgcg
     5701 cagaaaaatt tctgcgcgga gctttttgat ggtcggagtg gcgagacttg aactcgcggc
     5761 ctcttggtcc cgaaccaagc acgctaccaa ctgcgctaca ccccgatacc gatatcagca
     5821 agacagaatt atattgtctt tgagcggaat tgtcaagtat ttttcggaaa ctctttttca
     5881 attcttgtaa aaaacttcag actgttatag aatgtacgta cattttacgc acactctgtg
     5941 caggagcttt gatgtatgga caagaaactt gaacggattc tcccgctcgt acagaagccc
     6001 gcccggtaca ccggcggtga gtacggcgaa attcagaaag ataaaagtga agtcgatctc
     6061 cgcatggcgc tctgcttccc ggacacgtat gagatcggca tgtcgaacac cggcatgcgc
     6121 attctctatc aggtgctcaa cgaccttccc ggcgtgtggt gcgagcgcgt gttcgctccc
     6181 tggtttgata tggaccggca gatgcgcgaa aagggtctcc ccctctacgc gctcgaaagc
     6241 ggcgacccgc tcagcgagtt tgacgcgatc ggcttttctc tcggctacga gatggcgtat
     6301 acgaccgttc tcgaaatgct cgatctggcc ggcattccgc tgcgcagcgc cgagcgcaca
     6361 agcctcacgc cgctcgtatt cgcgggcggc agcgtttgct gcaacagtga gccgatggcg
     6421 gactttttcg atcttatgat catcggcgag ggcgagagcg tggacggcga agttttgcag
     6481 cttctgcgcg aggcgaaagc cgccggttgg agcaaggcgg aatttctccg ccgcgccgcg
     6541 ctcatcggcg gcgtgtacgt cccttccctc tatacgccgg agtataacgg cgacggcacg
     6601 ctcaaggcca tgcacgccgc gccgggtgcg cccgagcgca ttacgaagcg cattgttacg
     6661 gactttgaaa acagctgctt ccccgtggaa gcgatcgtcc cctccacgga gatcgtgcac
     6721 gaccgcgtcg gtcttgagct cttccgcggc tgcatccgcg gctgccggtt ctgccaggcc
     6781 ggacacattt accgcccggt gcgcagccgc agcgtggaaa aatgcatgga gtacggcaag
     6841 gaatctctcg ccttctccgg ctaccacgag atcacgctcc tctcgctcag cacgagcgac
     6901 taccgcgggc tgaacgagct ttgcgacgga ctgatggact actgcgaaag ccgcggcatc
     6961 ggtctctcgc tcccctcgct gcgcgcggac aacttctcca tggacatcat gcagcgcgtg
     7021 cagaaggtgc gcaaatccgg tctcaccttt gcgcccgagg ccggtacaca gcgtctgcgc
     7081 gacgttatca acaagaatgt caccgaggaa gaccttctcc gctcgtgcgc cgtggcgttt
     7141 cagggcggtt ggaacagcgt gaagctctat ttcatgctcg gtctgccgac ggagacggac
     7201 gaggatgtgc tcggcatcgc cgaacttgcc gaccgcgttg tgcattgctg gcgggaaaac
     7261 tcgcccaacc gcagccacgg gctgcgcgtg acggtgagca cgtcctgctt cgtaccaaag
     7321 ccgcacacgc cgtttcagtg ggagccgcag gtcacgcgcg aagagtatat gcgccgcgtt
     7381 acgctgctgc gcgacaacat gaaagcgcgc gccgttacct acaactggca tgacgccgac
     7441 acctcgctca ccgaggcggt gctctcgcgc ggcgaccggc ggctcggcgc aatgattgaa
     7501 aacatctggc ggcagggcgg ctttcttgaa tcgtggtcgg agggctttga tctcaagcgc
     7561 tgggaaaggg ccgtggagga ggaaggcatc tctttcccgt tctatgccaa ccgcgaacgc
     7621 tcgctcgatg aagtgctgcc gtgggatcat ctctatatgg gcgtgaacag gcgccactat
     7681 atccacgaat acgagcaggc gcacaagggg cttctctccc ccgactgccg ggcgcagtgc
     7741 tccggctgcg gcgctgccgc atttctgaaa ggaggccgct gcaatggata aac
//
LOCUS       DAWAAE010000097         7701 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4200_length_7701_cov_3.576641, whole genome shotgun sequence.
ACCESSION   DAWAAE010000097 DAWAAE010000000
VERSION     DAWAAE010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_7701_cov_3.576641"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1799 a         2207 c         2351 g         1344 t
ORIGIN      
        1 ggtcggcctt ggcgccagtg cccttgccgt ggagcaccgg gccgccgcgg gcgcccgcgg
       61 cgcgggcggc gttcatcaca agatcggtct gtccctcgct tgccacaacg acgatcagct
      121 catacgcata accggacggc ggagccgttt ttgcggattt gttctcttca cccatcagat
      181 atgccacggt tttccctcct ccaatgcttt tgaccggcac ggcgatcacg atgccgtgac
      241 ccggtacgcc gatgtgcagc cgccgcttct gcgcttcgat cagcgcggcg gtcttttcct
      301 cggtcgccgt ggacaggacg acccgttttt cgttcgattc gatcccgaga agatcgagca
      361 tgctctgcac cgccgtgccg cggccgtgca gcgtcacatt gagcggcagc gcgagctcct
      421 cgcatatgtc ctcaagcacg ccgagcgctt cgggaccggt gatggatatg acgtagttca
      481 tgcgctcgcc tcccaaagct cgatgatatc gtaatcgccg taaacggccg cttcttcctg
      541 cggacgcttt gcgatatgtt catagtagaa gccgacggcc tggatcgaaa gcagcggcat
      601 catggcgacc agcgccacaa cgccgaaggc gtcgcacagc acgtcgccgc cgagagcaat
      661 gctcgtgccc atcatgaact ggagcatgaa tgtcgccgtc atggggccgg aggcgacgcc
      721 gccggagtcg aacgcgatgg cggtgtagat atccggcaca aagaacgaga ggatcagcgc
      781 gagcgcgtag cccggcacga tgaaccacaa aagcgaaatg ccggtcacaa cgcgcagcat
      841 ggaaaacgcc atggcgagcg cgatcgcaac ggagagactg gccttgatcg ttttgccggg
      901 gatagcgccg gcgctcacgt cctcgatctg cttttcgagc acgccaacgg cgggctccgc
      961 cgagatgatg aaccagccga gcagcgccgc gagcgggatg agcagccagc tccgatcccc
     1021 ttcggcaagc gccgcgccca gctccgcgcc gagtgacgag aagccgacgt tcacgcccgt
     1081 gaggaacagc acgagaccaa cataggtata caggataccg accatgatct tccagaagct
     1141 ccggcggttc atacggaaca tggcaagctg gaaaatgagg aaaatgccga cgatcggcag
     1201 catggcgacc gccatttcct taaggtacac cgggatcgca ccgagaaacg ccgagccgat
     1261 cacggtcgtg ctcgcgtagg atacctccgt gagctccgca acgccggagc tgtcgcggta
     1321 aaagagcccg agaatgagca ccgcgaggat cggcccgatg gagcagagcg ccacgagacc
     1381 gaagctgtcg gccttcgcgc cgctgtcgct tcggatgttc gatacgccga gaccgagcgc
     1441 gaggatgaac ggcacggtca tcggcccggt cgtcacgccg ccggagtcga acgcgatgga
     1501 gagaaagttc ttatccgtaa acgccgcgag cgcgaatacg atcgcgtagc acacgagcag
     1561 cacccagcgc aggcggatac cgtaaaggat gcgcaccatg caaagcgaca tgaataatcc
     1621 tacgccagcg ccgacaacgg cgagaagcac ggtcttttca atgtgcggca ccgtaccggc
     1681 gagcacctga agatcgggct cggcaacggt gatggcaaag ccgagaagaa acgatacgcc
     1741 gagaatgagc ggcagatttt tcgttttcgt gagcgccgta ccgatacggc tgccgatgag
     1801 cgtcatagac tgctccgcac cgagcgagaa aagccccatg cccacggtga gcagcagcgc
     1861 gccgatgaga aacgtgagca gcagatcggt gcgcatcggc acgatacaaa gacacagtat
     1921 cacaacgatg acgagaatcg gcagtacgga tacggcggac tcgcggatct tttctccgag
     1981 cacggtgcgc gttttctcaa gagcggaacg tttgatcttt ttcggtttct gcacgcgcac
     2041 aaagcctcct ttaacaatct ttttttaaca agtgtataaa aagtatacgg gaatttcggc
     2101 gaaaaagcaa taaacgttct gttaaaaatg tcagcgggca aaaaatgctc cccggccaac
     2161 gccggggagc caaaaagcct cgcagagttt gctcccgcag gagcgaatag agttcaaaag
     2221 tcaaaaatgt ccgctgcggc cggagcctcc gccgccggtg cggccaacac tgccgccgcc
     2281 tccgccggag ggcttgggcg gctcgatgcg gcggcgcgtt tcggtgcggt gggtaaagcg
     2341 gtcggtctgc cgtgcgagac ggagcttgcc ggaaacgtaa ccgtctgccc gcgcctgcac
     2401 atacacgctt ttcatcttcc gcttgagaat ggagcatacg agaaacgcga tgaggattcc
     2461 cgcggcgcac gaaatgacga tcgccggtac cgggctgcgg cgcacggggt gcccctccgc
     2521 cgcgagcgca aggtactccc cgcacgcgga aagatagtcc gaaaagccgc cgtaccagtc
     2581 gttttcgagg aagttgtcga ggaatgtatc ctcgatctgc tcctgggcgt agctgttgaa
     2641 ggcgtattcg ctcttttcgc cgtaatagaa gtgggcgaag gcgctgtcgt tcatgcttaa
     2701 aaggaggatc gcgccattcc gctccgcgcc ggcgccgagc gagttgcggt gatagtaggt
     2761 atacgccgct tcatatgtgc cgcggctgtc gatgcggtag gtgtcgttca cggtgacaag
     2821 ataaatgccg acgccgtagc gctgcgcgat gcgctgcgcg gccttttcga gcgaggcggt
     2881 ctcgctcgcg gtgaggatac cggcgtcgtc cgttatgtaa gagagctgcg cgccgtccgc
     2941 gagcgcggcg ggggtgagag cgagcaggag aaggaaggcg agaaggaaag agcaaatttt
     3001 ccgtttcata tttttccctc cttatcgcag cagaaagctg agtgccgttc cgagaagcgc
     3061 gaccggcgcg gcaatagcgg cgaatgtccg ccagtatttt ccggcgtccg tcggcagatc
     3121 gccgaccatt ttgccggtct gcccgttcat ggcaaagagg aagctcttgc cgttccagcg
     3181 cgtcgagagc atccacaccg gcagcagcgc ataatgcacc ttgccgcggc gcagccggac
     3241 gtcctgcgcg cggggaatac acgtgtcgta cccgcttacg gtgcgcccga gcgcgtccag
     3301 caccgttttc cggcagcgct cgtcggcgcg gctttcgcag tcggcgaccg gaacatcgta
     3361 cttatctgcg agaaagccgg ggagatacgc cgaggaaaac ggtttgagtt cggcatagtc
     3421 gtagggctca atggagtcca tatgcgcgtc cggcatccgg ctcgaagcgt ccaccggcac
     3481 cttttcaaag ggcatcgaac cgtcgcggca gatctcaaaa tggtcggtct tcgtgacctc
     3541 ccaatcgccg gtgcggaaaa cgcgcgagcg cgtcgcgtcg tagcgcgcat gaccatccgc
     3601 ctcaccgtcg aacatccaga aggggacgta aacgccctgg atcttctcaa tctggttctg
     3661 ggcggaaaac gctctcggca ggaagggctt gtttttatag tgccgccgga gcgcattgat
     3721 cgcgtcctcg cggctgagct tgaagggcag aacatagtcc ggccttagcg ccccggcaaa
     3781 ctgccccggc acgacggtcg ggttgccgca gtacgggcag gcggtcgcgg cggtgttcgc
     3841 atcgcagatg agctccgcac cgcaggaagg acaggaatag gtcttcatcc cggcggcatc
     3901 cgcgccccag tcggagctca tggagccggt gtcccacgcg ccgctgtcat cgccgctctg
     3961 cgcggcagta tccgccgttt caaacgcctc ggcggccttt gcttcctttt ccttgtacag
     4021 cgcctcgatc tctgcaacgg tgtacgagct gccgcagtaa tcgcattcca gtttcccgga
     4081 cgcgccggaa tagtgcagcg gcccggtgca ggacgggcat tggtagttgg tcacctgtgt
     4141 cggcataggc actcatcctt tccgataaag aatgcttctg tttcccgcgg ggaacagaag
     4201 cattgtagca tattttcgcg ggaaactcaa cgctgaacgc gccgctggcc gatgcggagg
     4261 gcaatgttaa atacaataat aatgtagaag gagcgtcaat aaaacagcat cctccggctt
     4321 caacccagag agaagccaga gaatgctcta ttcactgcca gctgtcagcc gacctgcacg
     4381 tcgccgtttt tcagccgctg aacgatcgaa tgtacgagct gtatgtcgtt gtcggaaagc
     4441 ccgtcggtgc ggatggcgaa agccacgtcc agaccgagca gataatcggt ggacatatca
     4501 aaaatctgcg ccagctgcac cagcgactgc gtggagggaa cggaaatgcc catttcccag
     4561 gcgttcacgc acgagcgcgt caccccaagc attttggcaa gctccgtctg agtatagccc
     4621 tgcttctcgc ggagcttttt tattctttcg taaatcatga ctgtacctcc cggctcctgt
     4681 cgtggagtaa tatgatgtat ggtaagataa ccaaaatgat attgcattat cattttggtt
     4741 aattaaaatt gacaatcaaa gatcgttgtg gtttaatgaa ttcgtataaa aaaaataagg
     4801 cggatgccga aaacgaaaag acgatctgga tcgacgaagc ggcggatgta attaacggaa
     4861 acatcctgac gctgaaattc caggtactgg ataatgctcc ggatgggctg gcagaggtga
     4921 cggtcacggg cttcaaggca gcggctttgg atgagagcgc ggtgaatgcc aatatcgttg
     4981 cgggcggcgt aacagtcacg agccggattc cgggcgatgt gaatgacgac ggcgaagtgg
     5041 atatcttcga ctgtgtccgt ctgaagaagt atcttgccgg attcaacgta acgatcaacg
     5101 cgagcaacgc cgatgtgaac ggcgacggcg aagtggatat attcgactgc gtccgtctga
     5161 aaaaatatct tgcgggtatg agtgttgaac ttaaataaaa aggagcgcaa aatcatgaaa
     5221 aaaacattgg cggtaatcct cacaattctc atgatgacgt cgcttttgtg cacgggggcg
     5281 tatgccgatg gagaaaccat gaatttctcc gtcacatcgg ctacaggcag ccgcggcgat
     5341 gaagtgacga ttacggtcag cattaccagc tgcccgacta tcgtcggtat gcaggtaggc
     5401 atcagctacg atacgaacgt gctggaaaag gtaagctttt ccaacgcggg catgtccggc
     5461 ttggaagtcg ggacaagcgc tacctggctg tcggccaatg acactggttc gacgtacatt
     5521 ggaccgatgc tgacgctcgt attccgaatc aaagatactg cggcgtttgg accgagcact
     5581 gttacgatca atcccaaggt tagtgatttg gatgaaaaca gagtcccatg catcgttacc
     5641 cccggcacgg tcactgttac tgttgctgct catgatcaca tctgggacgc cggtacgacc
     5701 actcctgcga cctgcattaa gagcggacag acggtgtata aatgcacagt agccggctgc
     5761 accgagacca agattgaaac catcgccgcg acgggccaca agtgggacgc gggcaagatc
     5821 acgaccgagc cgacctgcac gaaggccggc gtgaagacga tctcctgcac aaactccggc
     5881 tgcaaagagg ccaaaacaga aaccgtgaag gcgctcggcc atgacatggg cgcggcgacg
     5941 gtgacgaagg ccccgacctg caccgaaaag ggcgagcaga ccacggcatg ccgccgcacg
     6001 ggctgcacgt ataaggaaac gaaggatatt gcaccgacgg gccacaagtg ggacaacggt
     6061 acggtaacga ccgctccgac ctgtacgaag gatggcgtca agacgttcaa atgcacgaac
     6121 agcggctgcg cggaaaccaa aaccgaggcc gtcgcgaaac tcggacactc tttcgggaaa
     6181 acagaagtga ttcagtctcc gacttgcacg gagcccggtg tccgcggcgc atactgcgag
     6241 cacaagggct gcacagaggt tggaaataaa tccacaatcc cggcgacggg ccacaagtgg
     6301 gacaacggta cggtaacgac cgctccgacc tgcacggcga agggcgtcaa gacgtttaag
     6361 tgcacgaact ccggctgcac cgcgacgaag accgaagagg tcgccgcgac cgggcacaag
     6421 tgggacagcg gcaggatcac gaccgagccg acctgcacga aggccggtgt gaagacgatc
     6481 tcctgcacga actccggctg caatgagacc aaaacagaaa ccgtgaaggc gctcggccat
     6541 gacatgggcg cggcgacggt gacgaaggcc ccgacctgca ccgaaaaggg cgagcagacc
     6601 acggcatgcc gccgcacggg ctgcacgtat aaggaaacga aggatattgc gccgacgggc
     6661 cacaagtggg acaacggcaa gatcacgacc gagccgacct gcacgaaggc cggtgtgaag
     6721 acgatctcct gcacaaactc cggctgcacc gcgacgaaga ccgaagagat cgccgcgacg
     6781 ggccacaagt gggacgcggg caagatcacg accgagccga cctgtacgaa agacggcgtg
     6841 aagacgattt cgtgcacgaa ctccggctgc aaggagacta agaccgaggc cgtaaaggcg
     6901 ctcggccaca gctggaagga cgcggaggtc gtcaaggcgg cgacctgcac ggcaggcggc
     6961 tcgaagaagg tagtttgcca gcgtgagggc tgtggcgcgg aggataccgt caacacgccg
     7021 gcactcggcc atgcctgggg caatgccaag gtcaccaagg cgccgacctg cacgcagccg
     7081 ggcgttcaga gcgtgacttg cacccgcggc tgcggcgcgg cgaccaccac ggcgatcccc
     7141 gcaacgggcc acaagtggga caacggtact gtgacgacgc agccgacttg cacgaaggac
     7201 ggcgtcaaga cggtcaagtg cacgaacagc ggctgcaaag agaccaaaac cgagacggtc
     7261 aaggcgcttg gtcacaagtg ggacgacgga aagatcacaa ccgaaccgac ttgcaccgaa
     7321 gaaggtgtca agacgatctc ctgcatgaac agcggctgca cggaaaccaa gaccgaaact
     7381 gttccggcgc tcggccacga ctgggatgag ggtaccgtga ccaaggagcc gacgaagaag
     7441 gaggccggcg aaaaggtgct tacctgccag aactgcggtg agaccgaaac ggaagtcatt
     7501 cccgttatcg cctccaccgg cgctccggtc tggctctgga tcgttatcgg cgtcggcgtt
     7561 gcggcaatcc tgctctggat catcctcgcc aagcgcaaaa aagaggacaa ggaagaagcc
     7621 aaacgctaag tccttataag agaaaatcgc tctgcctgat gtcgggcaga gcgatttttc
     7681 taactgagac tgggtctctc c
//
LOCUS       DAWAAE010000098         7685 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4215_length_7685_cov_3.796986, whole genome shotgun sequence.
ACCESSION   DAWAAE010000098 DAWAAE010000000
VERSION     DAWAAE010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_7685_cov_3.796986"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3672..3681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5640..5649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1552 a         2314 c         2145 g         1654 t
ORIGIN      
        1 ccatttttta ctcgcgcgtc agtccatctc cgggcgctcg ccgccgtaga aaacacggcg
       61 gctcagtctt cgcggctttt cgtgaacaga cggtaaagat tcagcaccgc cgcgtacagc
      121 acgcccgcga ccggtcagac gatccatgtg ttcttccagt ccccggtcgt gaagctccag
      181 ccgagataca cagccgcagc gagcatccag taggcagcgg aaagcgcctc ctccttttgg
      241 ctgcgctttt tctctgccgg agcgtaatcg ccggactgca aaattttctg catgcccgcc
      301 cagcggacgc ccgtcacaac gaaaaccgcc gcgccgaccc cggcaaggaa cagcattccg
      361 cacaccgcga ggatcatcgc aagatcgctc ccctccgcca tgccgatgaa gatcggcacc
      421 ggagagagga tgcagagcat cgtgccgacc atgttgcagc gagcgtgcgt gcttttatac
      481 tgccgttgcc gctcgctcac catgcccgtc acgccgtact ccgtgtcaaa cggttcctta
      541 tcaaggaacg catagggagc gtttttaaag ctgcaggaga gaaaagccgc gaccgccgct
      601 gcgataaaga ggaacagcac gataagccct gcggccgaag cggtgatttc cgaaatgccg
      661 taagccgggc tctcggcgag cgcgctgagg atcagcagcg gaatgaccga gacgatgcag
      721 agaaacgtcg ccgccgccat acgcacggag gcgtttttcc gcagggccag atattcgttg
      781 gcctcggcga gcgtgagccg ccggatggcg gaatcctcgc cggatgtgaa ttcctcactc
      841 tccaattcgt ccttgagaag atagtccgtc gtaacgccga agagagcgcc gagcatcagg
      901 atcttttcaa gatcgggcac ggtctgcgcg ctctcccatt tggacaccgc ctgccgcgat
      961 acgttcattt tctccgccag ctcttcctgc gaccagccgt tctttctccg cagccggatg
     1021 atcttgtccg ccaggatcat attcgtttcc tccgtttcgt ggcgcttatg ccgcgccgtg
     1081 atgttggagt caatgtaccg tttttccggg ttgcaagcca ccaattgaca gcttgaaatc
     1141 cgtcaaccac cggttgacaa agccgttttt gcaccggaag ccgccgtatt tccatggctg
     1201 tcacaggcca aagcacgccc gtccggtttt tcggatgatc tctgccatca aggtttcttt
     1261 ttccggatcg ttcatcgcca tagcgttttg gaagagccgg acttcattaa ggtatatctt
     1321 tttgcatccg gaggtcaggt acggagcgtc cgcgaggact ttcgtgtact cgccgcagat
     1381 ttccggaatt tcggaatacc gcttttcgta aaaggccgtt gccagccgga gccgctgcac
     1441 gaacacatcg gatatacggc attcgtgcgg cgagaaaagc gcccgatgac tcacctgagt
     1501 aaaaaaggca tcgggagaag tgatcgtttg aaagcacggg aaaatgtcat tcgatagtat
     1561 tgacatccat tcaccggcca gttccgccgc gtttcccgcg cctccatccc gctccgccgc
     1621 ccaaacggtc aatcgggtac cataggtcat atagcaaaac tccgtcggca catacagtgg
     1681 aagcacaaag cagtttgaat ataccgttcc ggtgggcatc tccagattca tgcagaaagc
     1741 catgtcgttc tctatccggc aaaagatgcc cttcttaaat gcaaagccat tcgctctctt
     1801 aaagctctgc gcacttttcc ggagcctttc ccaaaatgtc attccgtttg ctccttttcc
     1861 acggaagcaa tccttattct ctgaatttat cccgcacgtc gaagctgtgc caggaaccaa
     1921 ttcaaatccg tctccggatc atccgtgtcc tcatacagcg taacatgaag tttgaaaaag
     1981 ctccggaata ccgacgatat catccactgc gcggacaaag gataataatc cgcttccccg
     2041 tccgcgctgt caatttgaac ttcctttccg tctttcagaa cggtacagta tacatagcac
     2101 tggtacccgt tttggaaagg cgttctttgg aacccggcag tgtcattcgg aaaacacgac
     2161 cacataagac cggtcttcag agagacgccg ttttcctcaa agagtggctt atattccttt
     2221 attttttcga gatacagcgc cagcatatga tcctcatgga aaattatctg ctcatccagc
     2281 tcccttgtat atatcatttt cttcatggtt ttcccccatg ccgtctgccg attcaatcca
     2341 gatataaatc aaactgcatg cctgcgccca ttttgctcca gaaggaaacg ctttcgctcg
     2401 gaaacgctat caccggcaaa tcgttcgact ccgcatagac acgcaagctg cggatattgc
     2461 ttcgtgatct acgatcacag ttccgtctgc atcggttatt tctgcaatat aataaacttt
     2521 cgcattcgga ctttacggca tctcggagcc gttatgcagc cgctcctctg ataaaaaagg
     2581 cacgacgccc gggagaatga aggtctgtgc gaacattccg gttggaggtc gtgtctataa
     2641 aataattata cggatcgatc cgaaaaaagc aagaaaaatc gaccgaacgg ggcgcacggc
     2701 tatgcgcaga agcggtgctt gccgatctgc acgatcagcg ggcgagacca gatccactta
     2761 ctcgtcgccg tgacgggatt gaagtagtag atcgcgccgc cggtcgggtc gtagccgttc
     2821 atggcgtcgc gcgcggcggc gtaggcgctc tgcgcgaccg gctcgtcgaa ctgcccgtca
     2881 tcgagacagg agaacgcccc gcgctgatag ataacgccgg agagcgtgtt cgggaacgac
     2941 ggatgcgcga tgcggttcat cacgactgcg cccacggcga cctgtccttc gtagctctcg
     3001 ccgcgcgcct cggcggagat gagccgcgcg agcagcgcca cgtcgccgga ggtgttctgc
     3061 tcgatgagcc ccgcggacga gatgccgagc gccgcgagcg tctccgagcc cgccttgccg
     3121 tcggcggcga gaccgttttt ctgctggaag agcagcaccg ccgcctccgt gcggctgccg
     3181 taaacgccgt ccacctcggc gctgtagtag ccccagtcct tgagcttctg ctgaatttcg
     3241 gaaacgacgc tgccggtaga gccgcgcttg tacgacgccg cgtccgccct ctgcgcgagc
     3301 gtgatgaccg ctatattcac cgcgaacagc accgcgatcg caatgcaggc gcgttttatt
     3361 ccatattttg tcataaaaac ccctccaaac cgtttttcat agtttggagg ggtgcaattt
     3421 gttttatgat gtcaaatttt agtttttctt tgcgcggact tcctcgttca cgcgcgcgat
     3481 gaagtcatcg agagccatga cgccctcgtc accgccgcgg ctgcgcaccg cgaccgtgcc
     3541 gctctcggct tccttgtcgc cgagaatgag cagatacggg atcttctggc tgcggccttc
     3601 ggcgatcttc ttgccgatct tctcgttgcg aagatccttc tccacgcgca gcccggcagc
     3661 gtcgagcgcg gnnnnnnnnn ngcggcgacc ttttcggcgg cctcgttcgc gcggtcagtg
     3721 atcgtgagaa cgcgcacctg cacgggcgcg agccacaccg ggaacgcgcc ggcgtagtgc
     3781 tcggtgatga tgccgatgaa gcgctcgatg gagccgagaa cgacgcggtg gatcatgacc
     3841 gggcggtgct tctcgccgtc cgcgccggtg tactccagct caaagcgctc gggaagctgc
     3901 atatcgagct ggatcgtgcc gcactgccag gtacggccga ggctgtcggc cagatggaag
     3961 tcgagcttcg ggccgtagaa cgcgccgtcg ccctcgttga tgacgtagct cttgcccatc
     4021 tcggtcacgg cggccttgag cgtttcggtg gcgaaatccc agtccttcac atcgcccatg
     4081 tgatcctcgg gcatcgtgga gacctcgatc tcgtagcgca ggccgaacac ggagtagacc
     4141 tcgtcgaaca ggcggacgac attcttgatc tcgtccttca tctgctccca ggtcatgaag
     4201 atgtgcgcgt cgtcctgcgt gaagcagcgg acgcggaaga ggccgtgcag cgcgccggaa
     4261 agctcgtggc ggtggacaag gcccagctcg cccacgcgca gcggcagatc gcggtaggag
     4321 tgcggctcgg acttgtaaac gagcatgccg cccgggcagt tcatcggctt gatggcgtag
     4381 tcctcgccgt cgatgacggt ggtgtacatg ttctgcttat agtgctccca gtggccggaa
     4441 cgctcccaca gcgcgcggtt gaggatcatc ggcgtggaga tctccacata gccgtagcgc
     4501 ttgtgcacct gacgccagta gtcaatgagc gtgttgcgca gcaccatgcc cttcggcagg
     4561 aagaacggga agccggggcc ttcgtccatg agcgtgaaga ggccgagctc cttgccgagc
     4621 ttgcggtggt cgcgcttctt ggcttcctcg atgcgggcga ggtactcgtc gagcatgtcc
     4681 ttgctcggga agctcacggc atagaagcgc tggagcattt tgcgcttgga atcgccgcgc
     4741 cagtaggcgc ccgtgacgtt cgtgatcttg atgccgttgc ccttgatacg gccggtggaa
     4801 tcaagatgcg gaccggcgca caggtcggtg aactcgccct ggcggtagaa ggaaatgacg
     4861 gcgtcctccg gcagatcgtt gatcagctcg accttgtaag gctgctcggc cttttccatg
     4921 agcgcaacgg cctcggcgcg gggcagctca aagcgctcga gcttcagctt ttccttgcag
     4981 atcttgcgca tttccttctc gatggcttca aagtcgtcgg gagagaaggt gtgctcgctg
     5041 tcaatgtcgt aatagaagcc ctcttcgatc gcggggccga tggcgagctt cgtgcccggg
     5101 tacaggcggc agatcgcctg cgccatgacg tgcgagcagg tatgatggta cgttttgcgg
     5161 tattcgggat tttcccagct gtaagcgttc tcttccatga tgtttcctcc gatgtatatt
     5221 actcttttgc aaacaaaaaa agcacgcccg cgccaaaaac ggcacagggg tgctttgaaa
     5281 acacggttcc accctgttat ttcactcatt gacccataac gcggtcaaac gggcgggcat
     5341 ttcctcccac cggctccggg acggtcgata ccgggctcgt ccgcaggacg ctttcaccaa
     5401 agcgcgtccc tctctgcgct gacttctccg ggcgtttccc ttcaacgcct atgtcgggac
     5461 tatacaccca aaaaatgcgg gtgtcaagcc ccctgcgccg cacgctgcaa aagcgccgcc
     5521 agcgcgagca gcacaggctg atgtacaaga tagataagga gcgagcggcg gctcagccac
     5581 gcaagagcgc gcggcgcgct tgcgtacttc ttctccggcg gcatatcgcc gagccgctcn
     5641 nnnnnnnnnc ccccgccgca gccgcggcga aaaagagcgc gccccacggc agcagcggat
     5701 aataatccgc cgaggcgaac gccgccgtgc gcagcccgag cggaaaaagg aacggcacac
     5761 ccacccgcac acggatgggc agcatgaacg tcagtgcaaa aatgagcagc gccgcgccgc
     5821 aggcatagat tcccggcaga gcctcccagc gtctgcgcgc cgcggcatac agcagcatgc
     5881 acacgccgag caaatgcagt atgccccagt atacgggagc tccgataaga tatgtcaccg
     5941 cggacacaag aagcgccgca gcaagcacga tgagcccccg gcgcagaggg tgcttcgaga
     6001 gacgcgcgca tacgccggag atcgcgataa agctcccggc gatcatatac cgcgcaagct
     6061 tcataacgcc gctttgcata agcaaatccg gcacaagccc caccgacgca agatcaaaaa
     6121 gaaagtgcca tacgatcatg gcgagcagcg ccgctgcccg ccatgtatcg agaaagccta
     6181 tgcgtttttc gctcacttgg aaacgttgaa gcggaagagc gtcacatcgc cgtcgtgcat
     6241 cacgtagtcc ttgccctcaa gccggacaag gcccttttcc ttggccgcgg ccatgctgcc
     6301 gcaggctttg agatcgtcaa acgccacgat ctcggcgcgg atgaagccct tttcaaagtc
     6361 ggtgtggatc ttgccggcgg cctgcggcgc tttggtgccg cggcggatcg tccatgcgcg
     6421 cacctcgtcg ctgcccgccg tcaggaagga gatgagaccc agcagctcat aggacgcctg
     6481 aatgagacgc tgcaatccgg attcggtcat gccgagctct tccatgaaca cggcgcgctc
     6541 ctcctcatcc agctccgcga tctcctgctc aaaccgggcg cacaccggca gcacgcggct
     6601 gccctcggcg tccgcgagcg ccttgacctg ctggtagtag gcattgttct ccgccccggc
     6661 ggagaagccg tcctcgtcga gattcgcggc ataaatgatg ggcttgttgg tgagaaggtc
     6721 ggacgcggcg atgatctcgc gctcctcctc gtcggcctca aagctgcggg cggaacgtcc
     6781 gccgtccatc cactcgatga ggcggccgag gatctcggct tcctttttga atttcttgtc
     6841 gccgctcttg gcgtttttct ccgccttgtc ctgacggcgg cggagcattt cgatgtcggc
     6901 gaggatgagc tcgagatcga cggtctcaat gtcgctcacg gggtcgtgcg agcactcgtg
     6961 gcggatgatg tcggcattat caaagcagcg gacgacgtga acgatggcgt ccgtgttgcg
     7021 gatgtcgccg aggaacttgt tgccgagacc ttcgccctgc gacgcgccct tgacaagacc
     7081 cgcaatatcc acgaattcga tcgtggcggg ggtgtatttc ttcggctggt acatctccgc
     7141 gagatagtcc agccgcgcgt ccggcacagt gacaacgccg acgttcggct cgatcgtgca
     7201 gaacgggtag ttcgccgact ccgccccggc gttcgtcagc gcattgaaaa gcgtgctctt
     7261 acccacgttg ggcagaccca cgatacctaa tttcatagtt ttctctatct ccttattttt
     7321 caagggtttt cggcacttga acggcgctta ctgtaccatt actgtaccat tttgattttg
     7381 ctgcactttt tcaaattgta atacggcttt tgacatggaa tcattcgtca catgaacgta
     7441 ccgatccatc gtcgttttga tgctggcatg accgagcaat ttttgcagca cttttggctg
     7501 catcccgctt tcgattgcac gcgttgcata ggtgtgtctc agagcgtgca tacaaaagtg
     7561 tttgatctct gcttcatcgc agagcttata taagtgggta tcataggaac tgttctttgc
     7621 cggttccccg gtacgccagt tgataaaaac aagatcacgc atacacaggc ttgcagtttc
     7681 tcccg
//
LOCUS       DAWAAE010000099         7575 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4312_length_7575_cov_2.985505, whole genome shotgun sequence.
ACCESSION   DAWAAE010000099 DAWAAE010000000
VERSION     DAWAAE010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4312_length_7575_cov_2.985505"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1511 a         2331 c         2237 g         1496 t
ORIGIN      
        1 gatacatctt cataatatcg ccgcatttgg cgttgccgac ctcgccgacg ccgtccgcat
       61 cggggatctc gccgacgttg cggggatttt tgaaatgatc cataacctta tcgctgtaaa
      121 gagccatgat gcttgcctcc ttttatctca aagcatgaac tgttttttgc cggatgtgag
      181 gtctttccag accggggaca tgctgcggag atattccaca acacgcggga tctcgcgcag
      241 catatgatcg acctgctcct cggtgttctc ctcgcagagc gtgagacgca gcgagccgtg
      301 cgctacctcg tgcggacggc cgatggcgag cagaacgtgg ctcggatcga gcgagccgga
      361 cgtgcacgcg ctgccggagg aggcacagat gcccgcctcg tcaagcagga gaagaaggct
      421 ctcgccctcg atgccctcaa agcagaagtt cacgttgccg gggagacgct tcgtttcgtc
      481 gccgttgagc gccgcgtgcg gaatggtctt gagtccggcg atgagcttat cgcgcagagc
      541 gcttacatac gccgcgtttt tctcgagatt cgcgcacgcc tcggaaagcg ccgccgccat
      601 ggcgacgatg ccgccgatat tttccgtacc ggcacgtttg ccgcgctcct gcgcgccgcc
      661 ctcgatcacg ttcaccagag ggatgccccg gcgggcatac agcgcgccga ctcccttcgg
      721 gccgtggaac ttgtgcgcgg aaatggagag catgtcgatg ttctccgctt ttacgtcgat
      781 ggcgacatgg cccgccgcct gcaccgcgtc cgtgtggaag ataacgccct tttcgcggca
      841 gaccgcgccg atctccgcga tgggctggat ggtgccgatc tcgttgttgg cgtacatgac
      901 cgtcacaagg caggtatcct cgcggatggc gccgcgcacc tgctcgggcg tgacgatgcc
      961 gttttcatgg acgtccagca gcgtgatctc gtagccgtat ttctccagct tcttgagcgt
     1021 atgcagcacc gcgtgatgct caaacgccgt ggaaatgatg tgttttttgc ccttcttcgc
     1081 gccgatggct gccgcggaga gaatggcctg gttgtccgcc tcgctgccgc cggaggtgaa
     1141 ggtgatctcc cgtgcctcgg cgcccagaca cccggcgacc gtttcacgcg ccgcggcgag
     1201 cgcttccgcc gacttctggc cgaatgcata aaggctcgag gcgttgccgt accaggtgtc
     1261 cagatacggc agcatcgcgt cgatggccgt tttgctcatc ttcgtggtcg ccgcgttgtc
     1321 cgcatataca aacatggaaa aagctccttt caaaaatcct actgttcttg tatgtttaca
     1381 atgtagcatc ataaacatat attgtcaata ggttttttca aggagctttt tcgcgccggg
     1441 ttattccccg gaatcggtcg attttgcggc aatattgccg tttataaggt cggcgagcgt
     1501 gacgctctcc agataatcgt tcatcacgcg gtcaagctcc ttccagagcg gcagcgtgag
     1561 acagttgtcg gcgttcgggc actccgattc gccggacttg acgcaggata ccggcgctat
     1621 ggagccctcc gcaacgcgga ggatatcgcc gacggcgtat tcctccgggc agcgggcgag
     1681 ccggtagccg cccccgcggc cgcgctgcgt ctgcaaaaaa ccgcagcggc tgagcgcggc
     1741 gacgatacct tcgagatact tttccgatat gttctggcgc tcggcaatgt ccatcagcac
     1801 aacgtactcc tccccgccat tgcgggcaag atccgccatg acacgcagcg cgtagcgtcc
     1861 tttggttgag atcagcatgg atcgttcctc ctcatcggat cggataaaca tactatatca
     1921 caatgttttt aattttacaa ccggaatcgt cttgacaaac gcggtcaaat tgggtaaaaa
     1981 tgaaaggatt tttatcgcaa gaggttcaag cgtatgaagt acgtccgcca actcggcatc
     2041 attctgctcg tgtcatttct cggcgagctg ctgcattatc tgctgccgct gcccgtcccg
     2101 gcgagcattt acgggatcgt tctgctcttt gccgcgctgg aaacgcgcct tttaaaagca
     2161 gacgccattc gtgaaacgag cgcttttctg ctggaaatca tgccgatgct gttcgttcct
     2221 gcgggcgtgg gtcttctgga aaagtgggac gtgctgcgtc ccgtgtggct gccgtttgcc
     2281 gcgatcgtcg ttgtatcgac gttcacggtg ttcgccgccg cagggtgcac cacgcagcgc
     2341 gtgatgcgtc tgcgccagaa acgggaggat aaacgatgat gcgtgagctg atcgaaaact
     2401 ccgcctttct cggcgttgtc atcaccatcg gttcgtttga gctggggcgc gccatccgga
     2461 aaaagtggaa ctttgcgctg ttcaatcccc tgctgcttgc gatccttttc tccatcggat
     2521 ttttgctcct gttccgtatc gattacggaa gctatctcaa cggcgcgaag tacatcaact
     2581 ttctgctcac cccggcgacg gtatgccttg ccgtgccgct ctatgagaaa tttgagctgc
     2641 tgaagaaaaa cgcccttgcc ataacgctcg gcatcgctgc cggggtggct gcgagccttg
     2701 ccacggtgtt tgtgctcgcg gcgctcttcc ggctggactt ctctctgttc gccacgcttc
     2761 tccccaaatc catcactacc gcgatcggca tgaacgtttc cgcggagctt ggcggctacc
     2821 cctcactcac ggtcgcggcg atcattctga ccggcgttct cggcaacatc tgcgctgatg
     2881 gcgcgtgccg ggtgttccgc atcacgagcc ccattgcgca gggcgtcgcc atcgggaccg
     2941 ccgcgcacgc cgtcggaacg tcccgggcgc ttgaaatggg cgagatccag ggcgcgatga
     3001 gctcgctctc gctcatcgtg gcagggctgc tcaccgtagt gctcgccgcc ccggtcgctt
     3061 cgctttacgg catataagtc atatagggca aacgctcccc gccggacagg tcttccggcg
     3121 gggagcgttg tttatttttc ttcgtcgccg caggtatcgg tgatgatata ccggggtctt
     3181 cccttcactt cctcgtaaat tttggaaatg taatagccga tgatgccgag acttatcatg
     3241 atgatgctgc tggaaaaaag ctggataagg atcacggtgg tgaagcctcc gagcgcccgc
     3301 ccggagaact tctgcacgag cgcgatcgtg ccgagcacga gcgagacgag caacataatt
     3361 ccgccgagta ccgtgacgag ctgcatgggc gcggcggaga acgaggcgat attatccacg
     3421 gcgtactttg cgagcgccgg cgtggaccat ttggagcttc cgcctgcgcg cttttgcaca
     3481 tcgaagggga tgctcaccgt tttaaagccg atccacgagg aaagcgcgcg gaagaaggcg
     3541 ttccgctcgc gcatgttgat gagaacgttg accgccttcc ggtcgagcag cttgaagtca
     3601 gaggcgttcg ccatgtcgat gccggtcgcg cggctgatga gagaatagaa cgctttcgcc
     3661 gcgagaccgt gcgcgtggct ctccgcgccg cggctggcct tctgcccctc gacgatctcg
     3721 tagccctgct cccacaggcg gtacatctcc acgatcttct ccggcgggtg ctggagatcg
     3781 ccgtcgatca caacgcagca gtcacctttc gccgcggcca gtcccgcgag gatcgccgct
     3841 tccttgccga aattccggga aaaatgcagc ccgcggatgc cgccggacgc agagagcgcc
     3901 gtgattttct cccatgtggc gtcctttgag ccgtcgtcca cgaaaatgag ctcgcagccg
     3961 atcgcggcat cggacagcac accgcggatg accgaagccg ttttttccag aacggccccc
     4021 tcattataga cagggatcac taccgaaagc atcactccgc ctccttcgcc gtttccggct
     4081 cgcaccggat ctcaccgatg ccgagcacgt ccgccatagc gccgttcggc cagctctgcg
     4141 cgtcttccgt atcgagatat ttgagatcgt acagcgcgga atagcgcgtc agcgtctcga
     4201 tatacggcac cgtcacgctg ttctcgccgc gcacagccgc ctcggcgatg gcgctttcgt
     4261 tcgccctcac ctgaagatac acattcgtaa tatcggcaaa gccgaaaacg ccccaataga
     4321 gcgtcgacag cacgagcact gccgccgcgc acagcaccgg gagcttcgct ttgccggaga
     4381 agagctccga caaaagcacg gcgtctgccg caatgagcag caccgccgga aacgcgagac
     4441 accgctccgg ataatacgag gcgacgctca gcgcgaagtt ggcgcagagc gagccgagca
     4501 cgagcgccgc ggcgagataa attttctccc ggcgggcgtg gtaccaaagc gccgcaacga
     4561 gcgacacagc aaaaacaccg agcagtaccg ccagcgagcg gtacttctcc agcgcggcga
     4621 caagccccgt tccaagggac gcgagcgagc tctccgcgcc gcttttcatg ctctccgccg
     4681 ggcgggtcat cataaaaatg aagccgccga ccgacgcgag caccggcagc cacgggagga
     4741 cgccgttttt cttcctgcaa agcacacggc ggccaaaaag cagcagcgcc gaaaggaaga
     4801 tcgccccggc cgccacattt tccagatacg cgccggtata aaagcccgcg agcatccaca
     4861 aaatccagaa cagcggtttt ttcttcgcat cacgatccaa aaggcggatg tacggcgcga
     4921 gaaatgcaaa accgataacg catccccaac cgtaattgca cgccccgtcc agccagaaga
     4981 acacctgtcc gaacgccgga acaaacaccc acacggcgca gaagatcatc aatagaatga
     5041 ggttatcccg ctcgccctcc ggctgcgcga gccgggcaag gagcgcgatc agcgccgtga
     5101 atatcgaagc gtttactata ttaaataccg ctttcggcag cagcagaaag agctgcgccc
     5161 agaaatgggc gaagagccgt ccgttcatgg tctgcgcgtg cgccgccatg gagggaaata
     5221 tctcgccgaa gcgcgtgaga cgcccgccgg tcgcaaagct gtgcatatac gcccagtcgt
     5281 ccaccgcgta cggcgtgagg acatttccga gcagcatgag aagaaaaatt ccgaggagca
     5341 gcgcagcacg cactctcccg gagcggatcg ccggcatggt cagcgcctcc ttgcatggga
     5401 ttctttgctt atattataca caagcggaaa atgaagtcaa actccgcctc gattttttga
     5461 aaaagtctct tgacaatacc ccccaggggt atataatgca tattgaaagg cagggacggc
     5521 ccttggacga agaaaaaatg tgttgctgct gcaaaaaaac cgagcgaagc gaggaagagc
     5581 gccgcgcgct tatgaatcgt ctgagccgga tcgaagggca gatccgcggc atccgcggga
     5641 tgctcgagcg cgatgcttac tgcgcggaca ttctcacgca gtctgcggcg gtgagcgcgg
     5701 cgatgaacgg ctttaaccgc gcgcttttga acgcgcacct ccacaactgc gtcgtgcgcg
     5761 acatccgcgc gggagacgat tccgttgtgg acgagctgac ggagcttttg cagaagctga
     5821 tgaaataacc cgaaccgcaa tacaccacaa cagaaaggca aataccatga cgcaatactc
     5881 tgttacaggc atgagctgcg cggcgtgctc cgcgcgggtg gaaaaggcgg tctccgccgt
     5941 gccgggcgtt acctcgtgcg cggtgagcct gctcacaaat tcaatgggcg tggacggtac
     6001 cgccgctccg gcggacatcg tccgcgcggt ggaaaacgcc gggtacggcg caagtttgaa
     6061 gggggcgaag ggcgccgcgc ccgccgcagc agcggacgag ctggccgacc atgaaacgcc
     6121 gaagctcaaa aagcggctga tatcctcggt ggcggtgctg ctcgtactga tgtacttttc
     6181 catgggtcat atgtggggcg cgccgatgcc gcgctggctg catcataaca gcgtgggcat
     6241 ggggctcatc cagctcctgc tcgcgggcat cgtgctcgtc atcaaccaga acttctttat
     6301 cagcgggttc cggagcgccg taaagggtgc gccgaacatg gatacgctcg tcgccatggg
     6361 ctcggcggcg gcgtatctct ggagcgtata cgagctcttc acgctgacga tcgacccgat
     6421 ggcggaggcc gatctctatt ttgaatctgc cgccatgata ttaacgctca tcaccgtcgg
     6481 caagacgctg gaagcacatt ccaagggcaa aacgaccgac gcgctccgct cgctcataca
     6541 gctctcgccg aaaacggcgc ggctggagcg gaacggcgag gaggtcgtcg tcccggcgga
     6601 ggaggtcgtt ctcggcgaca ttttcctcgt ccgccccggc gacagcatcc cggtggatgg
     6661 cgtcgtgctc tccggcgcga gcgccgtgaa cgagtccgcg ctcacggggg aaagtctccc
     6721 cgtggacaaa gcggaaaacg acaaggtgag cgccggtacg atcaaccagt ccggctttct
     6781 gcgctgccgg gcgaccggcg tcggcgagga tacggcgctc tcgcagatca tccgcatggt
     6841 gagcgacgcc gccgcgacga aagcgcccat cgccaagacc gccgacaagg tcgccggcgt
     6901 attcgtgccg acggtgatcg gcatcgcagc cgttaccatt atcgtctggc tgctcgtcgg
     6961 gcagacattc ggatacgcgc tcgcgcgcgg catatcggtg cttgttatca gctgcccgtg
     7021 cgcgctcgga cttgccacgc cggtcgccat tatggtcggc agcggcgtcg gcgcgaaaaa
     7081 cggcattctc ttcaaaacgg cggcctcgct tgaagagacc ggccgcacgc agatcgttgt
     7141 gctcgataag accggtacca tcacgaccgg ccagccggaa atcaccgatc ttctcccggc
     7201 ggacggcgtt tccgaggacg agcttctgcg ctttgccgcg gcgctcgaga gcaagagcga
     7261 acaccccctc tcccgcgcca ttttaaacgg ctttgccttt cgcggcggcg agagtcccga
     7321 ggcggaaaac ttcgccgccg tgccgggccg tggtctcacc gcgaaaatcg aaggatacac
     7381 cgtctgcggc ggcaacgcgg acttcatccg cgaaaagacc gcgctcccgg agtccatcac
     7441 gcagcgcgcc gcggcgctcg cggacaaggg caaaacgccg ctctacttct cccgaaataa
     7501 tgagcttctc ggcgtgatcg ccgtggcgga cgttatgaag agcgacgctg ccgaagctat
     7561 ccgtgagctc aaggg
//
LOCUS       DAWAAE010000100         7516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4348_length_7516_cov_3.215119, whole genome shotgun sequence.
ACCESSION   DAWAAE010000100 DAWAAE010000000
VERSION     DAWAAE010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_7516_cov_3.215119"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1599..1608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1591 a         2285 c         2172 g         1458 t
ORIGIN      
        1 ccggaaggaa aaccggccat tcacataggc cgccgtttcc gcgagaaaat cgggattgaa
       61 gtccccgtcc gcggcgatcg ccggcgcagc gggcagtatc tcattcgccg cggcgtccgc
      121 cccgcctgtc aaaaggagtc ccagaccggg gacaaggcac acggcgagga acagcaccgt
      181 aaagataaga gagagagttt ttttcatatc gtctgccgcc ctcccttaaa actgctggta
      241 aatgaacggc gtgaagctcc cgcccgagag ggcgaggatc gccgcgagaa gcccgagcag
      301 cgcaagaacg ttccagacga tctcccagcc gcggccggtg caccgccgtg tgagcgcggg
      361 gacgaccggc agggagagga tggtgccgcc aacgagcgcc gcaatacgcg cgccggtcag
      421 tatctcctcc gcggcgagca gcccggcagc gcccgctccg gcaaaggaga acattcgagc
      481 gataatcagg aagccctgct gcacggagta cgcgcggaac atcgcaaagc cgagaatgac
      541 cgcgagcagc gtatagacgt gcatcacgat ccgcccgccg cgcttttggc gcagcttttc
      601 gatcggtgct ttcaaaatcc cctcgccgga aacgagcacg ccgtgccaga gaccccagag
      661 gatatatgtc cagcccgcgc cgtgccagag gccggtgcag aagaagacga tgagcttatt
      721 tcgcaccgtc ctccatttcc cgcggcggct gccgccgagc gggatataga ggtagtccac
      781 gaaccagcgg ttgagcgtca tgtgccagcg ccgccagaac tcgctcacgc tgccggagac
      841 atacgggtag ttgaagtttt ccggaaactc gaagccgaag agcatgccga tgccgatggc
      901 catgtcgctg tagccggaga aatcgaagaa gatctggaga cagtagctca ccgcgccgag
      961 ccacgcaagc cccatgccga gctgctccgg cgcgagggcg tatacggcat tggcgaccgc
     1021 cgccgcgctc tcggcgagca ggagcttctt caaaagaccg cgcacaagac ggcgaaggcc
     1081 cgccgccgtc atttccggcg agatcgggtg ctccgcgagc tgcgccgcgg catcctgcca
     1141 gcggacgata ggccccgcga tcaggcgggg gaagaacgca agatacagga agagcgtgtc
     1201 cgcactccgg cagacgcggg atggctcgcg gtacacatcc acgagatagg aaatgccgtg
     1261 gaacgtgaaa aacgaaatgc cgagcggcag cgcgaacggc tgcgaaagcg ccggaagatg
     1321 cagcgtttca agcacgaacg cgagatattt ataaaacccg agcagcgcca gattgcccgc
     1381 cgcggcgatg ccgcaccaga gcttcgctcg cttttcgtct ccggcgatga gccgcccgaa
     1441 gagatagtcc cagagaaacg ccgcgaacag cacggggagc gaggcaagac cgccgaagcc
     1501 gtagaacagc gcgctcgcta ttgcaagaaa aacgttcttc gtccggcgtc ccggcatgag
     1561 ccggtaaaga agaaacgccg cggggaaaaa gagaaacann nnnnnnnnta gatagcagta
     1621 ttatagcgta tttcccggtg cagcgcaaga tgcagctttt cggcacggct tgggggcgat
     1681 tcgacaaatt tacagtttct ccacactttt tttatggatt tttcaaaggg cgatgctata
     1741 atacggtcaa aagtaaaaac cggactgttt ggaaaggagc acatacaatg aacaacaaca
     1801 aagtgcaggc catcgtggag aaggtcaagg agctcgtgca gcgcggcaac gtttcccgta
     1861 tcgtcgtgcg gaagggcgag cgcgttctgc tcaacatccc cgtgaacgtc ggcatcgtcg
     1921 gcggcattgt ggcggtcgcc tccgccaagt gggtgctgct tgcgagcatt cttgccacgg
     1981 tcggcttcgg ctgcagcgtc gaggtcgtca aggaagacgg caacatcgtc aacgttatga
     2041 gcgaagagga cacccggcgc gtccgcaccg ccgcgcagag cgcggtctct tccgtgaagg
     2101 acgccgtgca ttttgaaaag gtcgtcgatc agacgaccga ggcaacctgg accgttcccg
     2161 aagaggaaac gtccgaggag ccggaagcgc acgccgagga agagcaccgc gaggagtaat
     2221 tccgccatac gcgaaaaagc acttgcatcg gcaagttaaa tgaagcgacg cgaaggaggc
     2281 ttgacgaaat aagtcccaaa attaagcaga aagcctcctt gacaaattcc gtcttgcttt
     2341 tttcttcctt gcatttccct gaaaattatg gtataacagt cccgcgtgag ttagcagcgt
     2401 ctaactcacg cgggactgtt atattaaaag gatactctca tgttcagcac actcagcaaa
     2461 caacaggaaa aacttttgca gacctctccc ctttttgaca gcctgacgcc ggagcagatc
     2521 cgtccggtgc tgtactgcct tcaggccgag gtgcgccgcg ccgcgccgcg gcagatcatc
     2581 attcagccgc aggagtgctc ttcggggctg ttcatcgttc tctccggcgc gatcgagcta
     2641 gtccatacgg acgccggcgg ccggaaaagc acgatgaaca ttctctatcc gggagactgc
     2701 cactgtgaaa acgtcgggct gctggacgct ctctccgcgc aggtgcaggt tctggcgctg
     2761 gacgaggtca ccatgataaa gctggacatc aaccgcattt tcgcccctga cagctgtcag
     2821 tgtccctaca agctggccgt tgtggaaaat ctggtgcgca ttctctctgc ggacaacgat
     2881 tttctgaagc tgaaggccac gctgttcagc cagaaaagtc tgcgcagcaa gctgctcata
     2941 tatctccggc atctcgcgca gaagcagaac accgacaccg tgtatgtccc cttcaaccgc
     3001 acaaagctgg cggattttct ggccgccgac cgcagcgctc tctgccggga gcttggccgg
     3061 atgcacacgg aaaagctcat cgccgtccgg ggcaaccgca tccgtctgac cctcggcagc
     3121 gaatctcttc tcccctgaca ttaaaaaaac gtcccgcggg tacggcagct ctctgccgtg
     3181 tccgcggggt ttttccgtcc gggggatcag ttccggacga tcatgaccat cattttgaac
     3241 cgggtaacgg cggcgacctt gtgcatcgcg ccgaggcgaa aggagaagct ctcgccctgc
     3301 tgcaccgtgt gcggcacacc ctcgttggtg atgacgccct cgccctccag acagagcatc
     3361 atggcgtcgc cgccggcggc gtgctccggc gtggcgcagc ccgcgtcaaa gctcataagc
     3421 gccattttca tatgcggatt atttacgata tccgcgttga cgatctttcc ctcctcgtag
     3481 gggagtagat ccttcagggc aaatacttcg tccgttttga taaggttcat acaaaatcct
     3541 tcctttgctt caatttcaag aaatacgcag ggatcctgcg ccagcacgcg aaacggcgtc
     3601 ttctgcggga tcagcagcgc gctgccttcc ggtagaacgc ggagcgaagg tgcttctacg
     3661 cgcgtctcgc ccgagagcga cagatagaga tacggatgcg gataaaactc ctcgctgata
     3721 tcggtgccgg cggcgatcga gaagattgtg gcatgctcct ccggagacat gaagagcgtc
     3781 tcggagatcg tcatgcccgc gcggggcgca tttgtcccgg cgaaggtgaa aacctgtccg
     3841 cgcttcatat cagtagtgcc ggtaggtctg cgccggagcc gtgagaatgt tggcgacgtc
     3901 ctcatcggtc agctccatgc cgtaccactt ctggtaatac tccttgacca tggcgtccat
     3961 atcgacgtct gtgaagagct ccggataggc cgtcttcgcc gaccaccaca tcgcaagcgg
     4021 tgtatcagtg cacggcggcc accagcggtg catgccgaag ggatacttga agacctgctc
     4081 ttccttcacg gccgtgatac cgctccagtc gtcctcgccc accgtgttgt tgtaaatgtc
     4141 cgccggggtc ttgtccgaga acgtcagaat gaagatcttg tcggggttcc actcatagat
     4201 ctgctccatg ttgacggagg agttcgtggc acgcgcgccg aggttttccg cgccgagcag
     4261 atccgtccac cacgccgcaa agctcttcgc gcccgcgatc acaaggccgg aatcgctgta
     4321 ggtattgata tagagatact tttccacgct ctccgtggga ttggcttcaa tgcggctgcg
     4381 gatctccgat tccacttcct cgccgtaggc aatgatgtcc tgcgcggaag aggtcatccg
     4441 gaacgtctgg gcataggtgt ccatccagga gcggagcgtt tcgagcgggg aggcgctgcc
     4501 gcccttattg ggatgcgtga gagccacggc ggggatgccc gcctgacgga tcggctcggc
     4561 gtccgactcg ctgtggtaga acaccacatc aggatcgagc tttacgagct cttccatgtt
     4621 cagctggccg ccggcgtaaa aatcgctgga gacgtccatg atctccgggc agtacttggc
     4681 gaacaccgag tattccacgc cgtttttcag atccggcgta acgcccacga gcttgctgat
     4741 gtcaccgccc atgaacacgg cgtagaccgt agcggcgggc agcggcccga ggatcacgac
     4801 gcggtcgatc tcgtcgggca cttcggtctc ctcgcccttg aagtccacca cgatatgcgt
     4861 gccggacgac gccggctcct ccgccggggt ctccgccgca gcgggctctt ccgccggggc
     4921 ctcggtcacg gccggttctt cacttgcctc gggcgcagcg ggctctgcgc cgcaggccgc
     4981 gagcgacagc accatcagtg ccgccagaaa aagtgataaa atgcgtttca ttggattcct
     5041 cctgaaaaaa tttatatttt aagtccttcg gaccaaaata cttacgtatt gcacagggaa
     5101 atggccgtga tagcctgata gtccatgccg tcgtactgcg catcgacgat atcgacgttg
     5161 accttgaaca cgcgccccat gacctcgcgc gtgatgacct ccgatggctc gccgaagtct
     5221 gccgtaccct cgcgtgagag gacgagcgcc cggtcggaga tgcgcagcgc gtggccggga
     5281 taatgcgtgt tgaagatgca gcacagtccc tcttcgcggg aaaggtggcg aatcgtttca
     5341 agaatgatga gctggttgcg gaaatccaga ttagattccg gctcgtccag cacaaggagc
     5401 ttcggctcgg cgcacagcgc gcgggcgatc agcaccatct gcagctcgcc gccgctgatc
     5461 tggttgcaga gcttgcggcg catatgcgcg atgccaagct cctccatggc gcgctcgcac
     5521 cgctcgcggt ccgccttttc cggcgttccg aacgtaccga tgcggctgct gcgtcccaaa
     5581 aggatcattt cctccactgt gaaggcgaag gggaaggatt ttgcctgcgg tacatacgcc
     5641 atctcctgcc agaggcggcc cggcgtcatg tcccggatgt tctccccgtc cagaaagctc
     5701 ccgccggacg tccacggcag cattcccatc atgctgcgca gcagcgttgt cttgccgacg
     5761 ccgttcggcc ccaggaccgc caggacctga cggctctcta cgcaaaacga gatgtttttc
     5821 agtatgggca cagtcttctt atagccgaag cagccgtttc tcacttcaaa ggtcatcgcc
     5881 agctccctcc cgtctttttc aaaagcgcaa tgaaaatggg cgcaccgatg accgccgtga
     5941 gaatggagat cgggatctcc gattccgacg cgctgcgggc cacggtgtcg agcaggagca
     6001 tgaacgccgc gccgatgctc gcactggcgg gaatcacgcg ccggttgtcg ctgccgaaga
     6061 gcatccggca ggcgtgcgga acgacaaggc cgacccagcc gacctgcccg cacatggaca
     6121 cgcacgaggc gatcaccgcc gtagccgcga cgatgatcaa tgcgcggatc tttttgagat
     6181 ccacgccgag agagcgcgct tcatcgtccg agagtgtcag aacgttgcac ttccagcgaa
     6241 gcatgatcag cacgagcgta ccggcgagaa tgaccggcat acccagccgc agccccttcc
     6301 acgttgcgcc gttcagactg ccgagcagcc agtaggtgat ctccggcagc ttgtcctcgg
     6361 aatcggccgt atactttatc aggctgacga acgcctgaaa gagcgaattg accaccatgc
     6421 ccgccagcac gagcatgatg atggaggtct cccggcggtt ctttccggct ttgtaggtga
     6481 tcagcatggc aacgacgccg aacgccatcg ccgtaagctg tacagccacg agcccgccgt
     6541 tgagtatcag cgcgagcgcc gcgccgaagc acgcgccgga cgctacgccc agcgtatccg
     6601 gggataccag cggattcgag agcaacgcct gcatcccggc tccggccacg gacagcccgc
     6661 cgcctaccag caatccgagc agcacacgcg gcagacgcac gccgatgatg atgcgctcct
     6721 gcggatcgac cgacgccgcc gctgcgtacc cgcgctttac cacgcggaaa ataatctcca
     6781 gcgcgtcgcc gagatgcacg gcgtaccgcc cgacggacag gctgaccgcc gccgcagcga
     6841 gcgggacaaa aatgagcagt gccacaaacg gtgtggcgcg aagtttgcgg agcaaggggt
     6901 tcatcgttcc tttcggttag cattgtctaa ccgaaaggat agcataaggc tccgggtctg
     6961 tctgttgccg gagccacagt cgttttattg ccttctgcac ccttttctgc cgttaaaagt
     7021 aaccccccag taaaactggg ggttcttcct atgcgggcaa agctctctgt tcctcgcgta
     7081 acgcgtaaac gtgtaatacg gtctgccagc tgcgcaggga ttgttacttg ccgcccgtaa
     7141 acgggccaat agaggctatc gtcatctgtt cacctaattc gtcctctttc cgttggttct
     7201 ttatatactc cgcaattcgg gtctcgttct ttcctaccgt atccacataa taccccctgc
     7261 accagaattc tctgttgcgg tacttgtact tcagttcccc gaattgctca tacagcatag
     7321 tgttgctctt ccccttcagg tatcccataa atcctgatat cgacagcttc ggcggtatct
     7381 ccacgaacag gtgaatgtga tccgggcagg cttccgcttc gatgattttc acactcttcc
     7441 attcacatag ttgccggagt atttttccta tcgctgcacg cttatctttg tagaatccct
     7501 ttcttcggta tttcgg
//
LOCUS       DAWAAE010000101         7435 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4398_length_7435_cov_3.243496, whole genome shotgun sequence.
ACCESSION   DAWAAE010000101 DAWAAE010000000
VERSION     DAWAAE010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_7435_cov_3.243496"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3773..3782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1398 a         2155 c         2324 g         1548 t
ORIGIN      
        1 ccgcaacgac gagaatggcg atatcggtca ccatcgcgcc gcgggcgcgc atggtggtaa
       61 aggcctcgtg gcccggggtg tcgaggaagg tgacggggct gccgttgacc atcacgcggt
      121 acgcgccgat gtgctgcgta atgccgcccg cctcgcccgc aacgacgttc gcgctgcgga
      181 tgcggtcgag cagggaggtc ttgccgtggt cgacgtggcc catgacgacg acgaccggcg
      241 cgcgggggac gagttcgtcc tcggtatcga cgtgatcgtc aataagacgg tcctcgaccg
      301 tcacaacgac ctcgtgctcc accttgcagc caagctccat ggcgacgagc gcggcggtat
      361 cgtaatcgat cacatcggac acggacgcca tcacgccgag cttgatgagc tgcttgatga
      421 cctcgaccgc cgttttcttc atgcgggagg cgagctcgcc gacgttgatc tcgtcgggga
      481 tgctgacctt gagctgctgc ttctttgcaa cctcaaactg caggcgctgc atcttgtcgc
      541 gctcttcctg gcggcgcttg gcgcttgcga cctgctgctg gcgctgcttg ttcttgttct
      601 ggatcttctc cttgccgccg cggcggatgt ccttgtcctt cccggcgttg ccggcgttgc
      661 cggcaatctt gtcgaagcgc tcgtcgtatt tcgagagatt cgctccggcg gagccgcggg
      721 tatcgacgac gcgcttttcc acgacctgct tcggctgggc gggcttctcc ggcttcggct
      781 tctgcggctg ctgcgccggc cggcgcgctg ccggcggtct tttcctgcgc gggagcgtcg
      841 ggcttcttct cttccggcgc cttggcgggg gccttcttct cctccggttc gggggcgaag
      901 atctcggcaa tgctgccgac ctggtgatgc tgcgtcagat actcgaaaat gagatccagc
      961 tcggcgtcct cgagaacctg catatggttc ttcggcgtcg tggcatagtc ggtgaggatc
     1021 tgggaaatat ccttggaggt catgccaaaa tccttggcaa cctcgtgaat gcggtattta
     1081 aaatcaagcg ccatacttat ttcctccttt ttccgctctc ggtgtgctta cggggcgcgg
     1141 ccttccgccg cttgagctta tcctcgcgct gggcgagaag ttcggctgtt tccttccagc
     1201 cgggaagctc ctcggcaagc gcggcggcga accgcgcggc gaggggcaga tcggtgaagc
     1261 agagcatgct gcatccgcgc ttgcccagca tggcggaaag ctccgccttc gtccagggca
     1321 gcgtctgcag cggtgcgcgg tgaccgtaca gaaaacccgc ggcgcgtttc tgcgcgttgg
     1381 gggaagcgtc ggcggcgagc atcaaaagcc gcccctttcc ggcggcgatc gccgtgccac
     1441 ttccttcctc gccgacggcg agcatcccgc ccttccgggc gagaccgaga tactgcgctg
     1501 ctttatccat cgcccgcctc cagttccgcc gtgagccgcg cccacacgtc ctccggtacg
     1561 gggacggaca gagcgcgctc gagcgcgcgc gatttgaccg cgcgcttcag acactcgctg
     1621 ctgcggcaga gataggcgcc gcgtcccggc accctgccgc acagatcgat ggagagctcc
     1681 ccctccggcg agcggacgac gcggatcagt tcggttttcg gtttttgttc acggcagccg
     1741 atgcactgcc ggagcggtac ggttttcggc aaaagcgctc ctcctgaaat gtatgtggac
     1801 tggcggggtt tgtttgatgg gtgggttatt cgttcgcggc gctctccggc ttgatatcga
     1861 ttttataacc ggtgagacgc gcggcgagac gggcgttctg gccctccttg ccgatggcga
     1921 gagagagctg gctgtccggc acgatcacgc ggcagctgtg gccttcctca agcacctcaa
     1981 cggagaggac ctccgccggg gagagcgccg aggcgatgta ctgctcggga tcctcgctgt
     2041 acttgatgat atcgatcttc tcgccgccga gctcgttgac gatgctcgcc acacgcccgc
     2101 cgcgggggcc gacgcacgcg ccgatcggat cgatctcggg atcggcggag tagacggcca
     2161 tcttcgtgcg gctgccggct tcgcgggcga tggagcggat ctccaccgtg ccgtcgaaga
     2221 tctcgggcac ttccagctca aagagccgct tgacgagacc ggggtgcgtg cgggagatga
     2281 gtacctgcgg gccgcgggtg gacttgcgca cctcgacgac atagaccttg atgcggtcgc
     2341 cctcggtgag ctcttcgccc ttgatgcgct cggtggcgga gagcatggcc tcggtgaatt
     2401 cgctgttgga gctgatcttg atggacacgg cgccggtgcg cggatcgatg cgcgtgacga
     2461 caccggtgag gatctcgtgc tccttggagg tgaactcgtc atagatgatg ccgcgttccg
     2521 cctcgcggat gccctggatg atgacctgct ttgccgcctg cgccgcgatg cggccgaatt
     2581 tcttcgtttc gaccgggatg cggacggtgt cgccgatgcc ggccttctta tcatagtgat
     2641 gcgcctcgtc gagcaggatc tgcgtggcgt cgttcacata ctcgctctcc tcaacgatgg
     2701 tcttgagaac gaacatgctg atggtctttt tattctcgtc catgatgacc tcgacattat
     2761 cctcgcagtc ggggttgtcg cgcttgaagg cggtcagcag ggcctgctgg atcttctcct
     2821 gcatgtatcc gcgggggatg cccttttcct tttcgatctg ctcgatggcg tcaaaaaatt
     2881 cggcgttcat tgcactcatc tatttttgct ccttacgtta tatcacgttg tgatttttta
     2941 cagcacgcgc agccgtacct gcgcgatctg ggccttttca aaggtggtct cttttccggc
     3001 gatcgtcaag gtaacgtcgc cgtcgcggta tccggagagc gttccgacaa aggacttgct
     3061 gccgttcacc ggcttataga gccgcacctc cgcgccgctg cccataaagc gcgcaaagtc
     3121 gccggggcgc ttgagctcgc gctcggcgcc ggcggagctg acctcgaaaa cgtagctgtc
     3181 cggaatgggg tctgcctcgt ccagcacggg atcgagcgcg cgggagagcg cctcgcagtc
     3241 gttgatggaa acgccgccgt ccttgtcgat gtacacgcgc agaaaccact gtccggcctc
     3301 gcggatgtat tccacatccc agagctccag acccagcttt tcggcgacgg gcgcggccag
     3361 cgcggtcacg gtctcggtga ctttactcat aaaaaatcct caatttccag tttctgacaa
     3421 attcttttaa atcaacaaaa agagtgggtt ttgcccactc ttcctgtttc ttacgttcgt
     3481 acgaggggat tataccaccc tccgcgcgcg attgcaagtg atttcattaa attaatatag
     3541 agtatacgga aaattttcac ccgtttaccc gttcattgcg gtgaggacgt tgccgtagtc
     3601 cacctgatag ttcgcgccct cgtaggtgcc gttgagcatg gacatggcgc ggttccaggc
     3661 gtcgccgccc tcggtatagg gctggggctg gtaattgttc agcgtgttcg gcccgagcac
     3721 ggtgctgtcg gggccgacgg tgctgctcag cgagcagggg atgaaggaat agnnnnnnnn
     3781 nngcttgagc gtgagctgcg cgatgccggt gtcgtaagcg ccggggtcgg tgccgttgcc
     3841 ggggttggtg tggccgccga aaataaagtt gccgagaccg tagaagataa cgccgccgtt
     3901 ataatcctcc gccggctgga gccggtgggc gtgcgtgcag tacacggcgt tgaagccggc
     3961 atccacggtg tagcggcaga gctcggtctg cacgtcggtc ggttcatagt acccctcgcg
     4021 gcccatgtgc aggcacgcga tggtgaagtc cgcgcccgcc tcctcaagcg atttgagccc
     4081 cgcggcgatc ttgtcgcgcg acgccgcgac gagcttttcc tgcgcctgcg cggagagaat
     4141 gctcatggcg ttgtaggtga gctggttata aaggcagtaa atgccgacct tcacgccgcc
     4201 gtccttttcg tagatatacg tctcgtcctc gcccgcgtag gcaacgccca cggcgtcgag
     4261 cgcggcggcc gtgtcgtcat aggcgttctg gccgaagtcc atcgtgtggt tgtttgccag
     4321 cgtcacgaac tccacgctgc ccgcggtgag gatgttcgcg taatccgccg gggcgagaaa
     4381 cacgaactgc tcaatggaat cgtaatactt gtccgagagg ttgcactcaa gattggcgat
     4441 cgtgagatag tctttttcaa agtactgcac ggttttggcg aagggatagt cgtagttttt
     4501 cccgacgacg gtctgcagcg ccgtgcccca cccgcggcgg gtctttgcct cggcgagcgt
     4561 gcagtcgccc accatggaaa cggtgaagta ttcggggctc ggctccggcg tcggttccgg
     4621 tgtcgcggta ggctccgcgg tgggcgcagc cgacggttcg ggcgtgagct cggggccgga
     4681 ggttggcggc acgggcgttt cgctcgccgc cggcgcgagc gtttcggcgc tctccgcgtt
     4741 atttaccgaa acggcgcgca cggcaacgag cacaacgagc gcgagcgcga gcagacagaa
     4801 aacgatcagt gccggaacgg atacccggcg ggaaggacgg tgttgcatgg cgtatctccc
     4861 gtgttattcg gtgaaatgga tatggttgtt gatgtgatcc tgacagaagc agtgataccc
     4921 cgcgcagcgc gagcagtagc ggaactccag ctccggatgg tccgcgtcgg tgcggccgca
     4981 cacggcgcat ttgtagcggt acggggcgct ctgctgctcg cggcggatgc gctgcgcctc
     5041 gcgcttgaag ttcacggtct tgcggatctg cccgggccgc cggaagtgta cggagccgaa
     5101 gagcatatcc ccgcagaaga gcagatagtt gagcgtcgcc acgaccggca gaagattgag
     5161 cgggaagcgg agcgcgaaga tctcatagac gaagtacgcc gcgttcgcca cggcgagcca
     5221 cttgatcttg atcgggatga tgtacatgaa cagcacccgc tggtcgggat agtacgttgc
     5281 gaacgagaag aacatcgaaa gatagatgaa ctccgcgccg atgctcatcg ttatgccgcc
     5341 gaacagatag acgagaaagg cgtacaggat cgtgaacacc ataccggcga ggaagtacat
     5401 gttgaacttg gcctgtcccc actcgcgctc catcgaggtg ccgatgaagt aatagaaata
     5461 gaggaacagc agcccccaga tgccgccggt gtccgggatg agcacgaagg tcaccagccg
     5521 ccagatctgc ccgcgcagga tcatctgcgg attgagcgcg agaaaatagc gcaccgtgcc
     5581 ggtgcggtcc atcagcgtca gcagccagac gacggcgttg ccgatgatga gatagcgcat
     5641 gagattttcc acgccgaaac gcggatggcg gacgcagtag cgctcgaccc aacgcatgaa
     5701 tgctttcaaa tcaaaactcc tttccagaga aaaactgtaa cggaatactt aattatacca
     5761 catccggacg aaatttcaat ccgggagttg gccgtcttat actttcctcc gaaaggggga
     5821 aacaaaatga atgtttatgg ctatatccgg gtgtccagcc gggatcagaa cgaggaccgg
     5881 cagcgcatcg ccctgcacga gcggggcgtg gaggatggtt ttatttatac ggacaaggta
     5941 tccggcaaag attttgatcg cccgcagtac aaaaagttgg tcaaaaaatt gaagccgggc
     6001 gatttgctca tgatccagag catcgaccgg ctggggcgca attatgaaga ggtgcagaat
     6061 caatggcgcg tgctgaccaa ggaaaaggag gcggatatct gcgttctgga tatgccgctg
     6121 ctggacaccc ggcagggaaa ggacctgatg ggaacgttca tcgccgatct tgtgctgcaa
     6181 atcctgtcgt tcgtggcgca gaacgagcgg gagtttatcc gaaagcggca ggcggggggc
     6241 atcgccgccg atcccactgc cggagaattt ccgcgcggcg catgcggcat ggagagcgaa
     6301 aaagctgacg ctccaacagg cggcggacgc ctgccatatg aaggtcggaa cgttctacga
     6361 caaggcggtc aaactggaaa aagccccatg accggcgctg ctgtgcgttt ggaaaagtgt
     6421 actttttcat aaaaggaatt atatcacatc tctttacgta atgcgacccc gaatccgctt
     6481 ttttctccca attttttcaa tatcgaaaaa gtacacctta ttgcaaaagg agggagagaa
     6541 tggcggaaaa acaggtcaag tccagacagc gcgttgccga ccatggtgag gtctttaccg
     6601 ccgagcggga ggtcagggcc atgtgcgatc tggtgaaatc ggaaacagag cgcatcgatt
     6661 cccggttttt ggagccggcc tgtggcgacg ggaattttct ggcggagatc ctccggcgga
     6721 aactcgccat agtaaagcag aactacgccc cgcgccgcgg cagctgtcac gactacgagc
     6781 tgtatgccgt gcggtcggtc atgagccttt acggggtgga gcttctgcac gacaacgttc
     6841 tcgcctgccg ggaacggctc tttcgcatat ttgacgaaga gtatacctcc gtctgcaaaa
     6901 agaacgcctc cgacgaggtg cgcgccgttg tccgctatgt gctcgaaaag aacatcctgc
     6961 agggcgacgc gctcacgctc gtgcaggtgg acggggacgg gcagccgacg gacaagccca
     7021 tcatctttgc cgagtggacg ttcccgatga acgacgcgcg ggtaaagcgc cgggattacc
     7081 ggctggatgt ccttttaaaa gaaaaccccg acgagagcac ccacgatacg cagttttcgc
     7141 tctttgagga cgacgcgatg aacaccggct tctgggaaac ggaccccgtt accagacagc
     7201 ccatccccaa gcccgtccgc gaataccccc taattcactt tcggaggata acggagaatg
     7261 atgaacagct ataaccccga tgtgctctcg tgtctggcca acctctccaa cgacgaggtc
     7321 ttcaccccgc cggaggtggc aaacgccatg ctcgacctgc ttccgcagga gcttttctct
     7381 gacccgaaca caacgtttct ggatcccgcc tgcaaatccg gcgtgttcct gcggg
//
LOCUS       DAWAAE010000102         7417 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4411_length_7417_cov_3.225618, whole genome shotgun sequence.
ACCESSION   DAWAAE010000102 DAWAAE010000000
VERSION     DAWAAE010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4411_length_7417_cov_3.225618"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1207..1216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1438 a         2398 c         2080 g         1491 t
ORIGIN      
        1 gcccgaaccg actgcgacgc ccgagccgac gcccgacgcg ccgctcgtca accccctgac
       61 cggcgagacc gcgaccaagg actacagcaa gacacgcccc gtggcgatca tggtggagaa
      121 caaccactgg gatgcgccca tcacgcccat tcagcagggc agcatcagca aggccgccat
      181 cgtttacgaa gcgcaggtcg agcagatcac ccgcaacatg ttcattttca tggacctgga
      241 cgatgtggac aacatcaccc cgatccgcag ctgccgctcc tactttgtca gcaccgcgct
      301 ttcctatgat gcgatttttg cccactgcgg cttcagcgcc gacggtctcg aatactccgc
      361 gccgatgctc gcgaatcttg tggacaacga cgatatcaac atcaacgagg ataacggcac
      421 gggcttccgc ttcatggagt acccgtactc cggcggcgta cacagcatga cgaccaccgg
      481 cgagcgcctg cagaagttca tttcggaaaa cggcacccgc accgagcaca acaccgattc
      541 ctatgactac ggcctgcgct tcaccgagaa cgccgccccg acgggcggaa gcgttgccaa
      601 caacgtccgc atcgtgttcc ccggcacaaa gatcacgagc tttgagtaca gcgccgacaa
      661 aaacggctac accggcttca actggaacgc cgccatcgcc gacgccaaca caagcgaagc
      721 cgccgtgttc cagaatcttc tcgttctcgc cacctatacg cagatcggca tcgacgatca
      781 ttaccacacc gcgatgaaca cctacgagtg tgaaggcacg ggtctcttct tcaacggcgg
      841 ctactgcgag cccatcacct ggaagcgcgg cgccgtcaac gagccgttcc attactacac
      901 cgccgacggc accgagctcg agctcggcat cggccacacc tacattgcgt tcgtatccag
      961 ctcctacggc ggagccacct acaactgatt ttccgcaaac atcaaaagcc ctccggcagc
     1021 agccggaggg cttttcgttt ctcatgggcg aagcagagct gcgccgttgt gcggaaaagg
     1081 ggtatcgtat tcagatacgc gcgacgccgg ttcttcttgc ggcttcggcc acggccttgg
     1141 ccactgccgg gccgacgcgc ttgtcgaacg cctgcgggat gatgtagtcc gcggagagct
     1201 cgtcctnnnn nnnnnncccg tcgatacgac cgccgcgccc gccgctttcg cctcgtccgg
     1261 gaagatctcc ggcgtcgggt tcgcacaggc gaagatgatc gggtcgcggt tcatcgtctt
     1321 caccatctcg cccgtgagca ttttgggggc cgagacgccg atgaacacgt ccgcgccggg
     1381 gagaacatcc gcgagcgtgc cgcgcaggtg ctgcgggttc gtcacgtgcg acatctcttc
     1441 cttgatccag ttgagacctt cgcggccttc atagatcgcg cccttacggt cgcagagcgt
     1501 cacatccggg aagccagccg agagcagcag cttcgtgatc gccgtcgccg cagcgcccgc
     1561 gccgttcacg acgacgcgca cgtcctcttt cttcttccct acgaccttca gcgcgttcgt
     1621 aaggcctgcg agcgtgatga ccgcggtgcc gtgctggtcg tcgtggaaga tggggatgtc
     1681 gcatttttcc ttgagcttgc gctcgatctc aaagcagcgg ggcgcggcga tatcctcgag
     1741 attgatgccg ccgaacgagc cggagatgtt gtacaccgtc tgcacgaact cgtccacgtc
     1801 cttcgtcttc acgcacagcg ggaacgcgtc cacatcgccg aacgacttga agagcacgca
     1861 cttgccctcc atgaccggca taccggcctc gggaccgatg tcgccgagac cgagcactgc
     1921 gctgccgtcc gtgatggcgg cgcagagatt ccaccgccgc gtcagctcgt agcttttctc
     1981 aatatccttc tggatctcca gacatggctg cgccacgccg ggcgtgtacg cgagcgaaag
     2041 atcgtccttc gttccgaccg gcacggtcgc cttcacttcg atcttgccct tccactcccc
     2101 gtgcagacgc agcgattcct ttgcgtagtc cataatgatc tcctctaaaa agggcgcgca
     2161 ttgctgccaa tacgcgcctt gtctttttct cttacttcgt gccgaaaata cggtcgccgg
     2221 cgtcgccgag accggggacg atgtagccgt gatcgttcag gatcgggtcc atggcggcga
     2281 cgtaaacgtc gacgtccgga tgatccttct gcatcttctc ccacgccgga ggcgcgccga
     2341 tgatgcacat gagcttgatg tgacggcagc cgtgctgctt gaggaacgtg caggccgcag
     2401 aagcgctgcc gccggtggcg aacatcggat ccaccacgat cacgtcgcgc tcggagacat
     2461 cgggcgggag cttgcagtaa tattcgaccg gttcgagggt ctccgggtca cggtacaggc
     2521 cgatgtgacc gaccttgcag gacgggatca ttgcaaccat gccgtcgacc atgccgagac
     2581 cggcgcggag gatggggacg atggcaagct tcttgcccgc aagacgcttc accgttgcct
     2641 tggcaacagg cgtttcgatc tcaacctctt ccagaggaag gtcgcgggtc gcctcatagc
     2701 acatcagcat ggcgatctcg ccgacaagct cgcggaattc cttcacgctc gtgttcttgt
     2761 cacgcaggat cgacagcttg tgctggatca gcgggtggtc gaatacatgc agctcactca
     2821 tttttcttgc tcctttgtag tgtagttagc ttttattttc tcaggcttcg ttcatgcggc
     2881 tctgccgctc gcgttctgcc tgggaaagac gaagatatac cggcagcagg tcttccgctc
     2941 cggtcggggt tttgctctgc gccgcgagac acacgccgaa cgctgtctgc cagcggagct
     3001 cctcgggggc cacggtgcag aaaacgccac gttccttcag cgccttttcg cagatcgccc
     3061 agccgtcgcc gaggacccag acgctctttc cgctgccgga gaaatcttcg gcaagcgcgt
     3121 cggcggcgat ggcgcgatcc tccgtcagac ggacgagagc accgttttgc acggtgaaga
     3181 gcgcgttata aagctggctg cgccgcgcgt ccatggcggg gcagacgatc gtcccctccg
     3241 gcacgaagcg ggcgctctgc gccatggctt ccagcgtgga cacgccgacc gccggaatgt
     3301 ccagccccca gcagaggccc ttggcggtgg atatgccgat gcggataccg gtgaacgagc
     3361 ccggcccctg cgccacggcg accatgtcaa tggcgtcctt cggcgtttcg gtgttggaga
     3421 gcatattctc gatcatcggc aggagcgtcc ggctgtgggt aaggcccgtg cgctcatagc
     3481 tctgcgcaag cagctccccg tcgcggcaga gcgcggcgga gcaggctttc gcggaggttt
     3541 cgattcccag tatcagcaaa ggctcgcccc ctcgatcgtg atctttcgtg cgttgtcgga
     3601 gcatttctca atgctcacgc ggatctccga gccgtcgaat gcctcgggga cattctcgct
     3661 ccactccacg gcgcacacgc cgccgcggtc caggtaatcc tcccagccga tgtcgaacag
     3721 ctcgtccgcg ctgccgagcc ggtacatatc aaagtggaac aggtcccgct ttccgtaaag
     3781 ctcgttcaca atggtgaacg tcgggctcgt aacagccccg tctacctcca gaccgcgcgc
     3841 aagaccgcgc acgaacgcgg ttttccccgc gcccagctcg ccgtacattg cgatcacaac
     3901 gccgtgctcc ggcagcgagc gcgcgagctt ttcgccgagc gcttcggtct cggcttcgga
     3961 gtggctgata aattccataa tccgtctgcc tctgtctcaa aaaactcaaa cttctctaac
     4021 ttggcattat acaccgagca tttgccggag taaagacctt ttcctgcctg ttgcagaaaa
     4081 aagaatgttg tgatagaata gattgaaatt atatgtataa ggggaggtgc cgccgtgctg
     4141 gaaagactca aaaaactccg cattgcgccg atcatccaat atatatggag ccgtacctcg
     4201 gacttcttca aaaagggcga tatgttcctg ctcgtcatgt gctgcatatg cacatgcttc
     4261 ggcatcacgc tcgtcggcag cgccacgaag tcgtatccgt cgcacacgtt cgtgccgatt
     4321 caggtcttcg cgtttatcct cggtcttttc ctctactatg ttctgaccgt tcttgacgtg
     4381 agcatcattg caagccgctg gaagctcctg atcggctttg agacgttcct ccttctgctg
     4441 ctcatcccgt tcggcgtttc gggcgatacc ggcaactctg gctggctgcg ctttttcggc
     4501 atcggcattc agccttcgga gatcgtcaag gtcgtgttta tcgtactgat ggccaagcag
     4561 atatcctatc tgaaggaata taagaacctc aactcggtgc tctccattct ccagcttgtc
     4621 gtccatttcg gctacacatt cgtactgctg atggtcacca ccaaggacct cggctcggcg
     4681 atcgtgttcg gcgccatttt cgccgttatg ctgatcgtgg cgggcgtcag gctctactgg
     4741 ttcctcatcg gcggcgcggc gatcgcgctt gcgatcccgt tcctctggaa caacttcctc
     4801 gatgaatacc agcgcctgcg tattctcgcg ccgtacgatc ccagcatcga ccctgacggc
     4861 tggggcatca catggcagac gactcgaagc aagtatgccc tcatgggcgg acaggccacg
     4921 ggtctcggtc tcggccacgg cgcacagacg cagtccggcg cgctgaccgg caagcacacc
     4981 gacttcattt tcgccgtcgc gggcgaagag ctcggcatgg tcgcgtgcgt gctcatcatg
     5041 cttctgctct ccgttgtcat catccgctgc atgatggtgg ggctgcgctc gaacaacacg
     5101 atgagcatgc tcgtatgcgt cggcgtggcg gcgagcctct tcttccagat gctcgtcaac
     5161 accggcatgt gcatcggcat cacgcccgtt atcggcatca cgctcccatt tttcagttac
     5221 ggcggttcat cgctcatgtc cacattcggc gcggtcggcc ttgtatccgg cgcaaaatac
     5281 cgaccaacgc cgctccaatt ccaccgatat taaaagttca tattagatca tagggggaca
     5341 ggaaccatgg cacacagacc gaacattctt gcgctcgcat ccaagatcag tctcgaaagt
     5401 ctgacgtaca ccggcattac ctacagcgat cccgagtaca gaatccttga tcccatcgtc
     5461 gatgacgaca tgtgttccgt tatgatgcgc atgcgtctcg aaaccgactt ctccgcggag
     5521 gatctcgcca aaaagacgag aaagagcctc gatttcgtgc agacgcagtg tgacaagctc
     5581 gttaaagcgg gcgttatccg cacgcgcgtc cgcagcggcg ttctctgcta ctattacccg
     5641 atctgggtac ccggcatcat ggagggcatt ctctccaacc gcgagcagtg cgacgcgcac
     5701 cccgagctcg gcgcgtgttt tgaggagtat acccgcatcc gcgtcggcat gctcgcccct
     5761 atgctcggca acggcgtgaa tttcatgcgc gttatgcccg ttatgagcgc catcgaaaac
     5821 gacacgcaca aagcctccta tgacgagatc tccacgctga tcgaaaaggc gtgggcgatc
     5881 tccgtcggcc cctgctcctg ccgaagatcg cgccgtctga tgggcgaagg ctgcggccat
     5941 ctggaagagg atatgtgcct ctatctcaac gacaacgcca tcagcttctc caagaacggc
     6001 gagcaccgcc tcatcaccaa ggaagaagcg tacgagatcc tccggcgcgc cgaggacaac
     6061 ggccttgtcc acgaggtgaa ccaggccctc ggctttgacg atgtcaccgc catctgcaac
     6121 tgctgcggct gctcgtgcta cgcgctgcgc atcgccgagc tgttccgttc gccgaacggc
     6181 gtcagctcca acttcattgc cagagtggac aaggataaat gcgtcgcctg cggccagtgc
     6241 gtggaaaact gccagacgaa cgccgttcgt ctcggccaga agcactgtct caccgattcg
     6301 cacatctccg acgcctacgc atccgacaaa gagatcccct gggataagaa aagctacaac
     6361 atcgattacc gcaccacccg taccgacacc atgccctccg gcaccgcgcc gtgcaaggcg
     6421 gagtgtccgg cgcatgttcc ggtgcagggc tacataaagc tcgccgcgca gggccggtat
     6481 acagaggctc tcgagctcat caagaaagag aaccccttcc ccgccgtctg cggccgcatc
     6541 tgcaacaagg catgcgagga cgcctgcacc cgcggcagcg tcgatgcgcc catcgccatc
     6601 gacgatatca aaaaattcat tgccggaaag gatctggagg cggagcaccg ctttgttccc
     6661 aaaatggtca accagaccgg caagccctac gaggaaaaga tcgccgtcat cggctccggc
     6721 cccgcgggac ttacctgcgc ctactatctt gcgctcaagg gatatcccgt gaccgttttc
     6781 gagaaggaaa aggagctcgg cggcatgctc acgctcggca ttccctcctt ccggctggaa
     6841 aaggacgtca tccgtgccga gatcgatatc ctccgtgatc ttggcgtaca gttcaaaacc
     6901 ggcgttgccg tcggcacgga catcacgctc gatgcgctgc gcgccgaggg ctttaaggcg
     6961 ttctatctcg ccgtcggcgc gtcccgcggc gcgaagctcc gctgccccgg cgaagagctg
     7021 ccgggcgtta tgaccggcat cgatttcctg cgcgcggtca atctcggcga agcgcccgcg
     7081 atcggaacgt ctgttgccgt catcggcggc ggcaatgttg cgatcgatgt ggcccgcgcc
     7141 gccgtccggc tcggcgccga agtcaccgtc gtttaccggc gtgaccgcga ttccatgccc
     7201 gccgcggacg acgagatcgc cgaggccgcc gaagaaggtg tttccttccg cttcctcgcc
     7261 tcccccgcgg aaatcctcgg cgacggcaaa gcggaaacgc tcaagatcga gctcatggag
     7321 ctgcgaggcg gaaagcccgc cggcaccggc gtttatgaaa cgatgaatgt ttccgccgtc
     7381 atctccgctg tcgggcagga gatcgagttg aacggca
//
LOCUS       DAWAAE010000103         7315 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4472_length_7315_cov_2.602479, whole genome shotgun sequence.
ACCESSION   DAWAAE010000103 DAWAAE010000000
VERSION     DAWAAE010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_7315_cov_2.602479"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1292..1301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5284..5293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1437 a         2318 c         2100 g         1440 t
ORIGIN      
        1 agcggagata ctccgccagc tctcccggaa gcatggattt tatatcgatt ttttcggtca
       61 gttcagccat ttcgtctcat acggcaatag aaaaagccgt ccgtatcata ttcctgcggc
      121 aatatgagcc gctgctcctc gcggacaaag tccggatgat cgtgaagaaa tcgttccgca
      181 acatcctcgt tttcctcatg gaaaacggtg caggtggaat aaagaaggat cccacccggc
      241 cggacgcacg cggcaagccc gtccgcgatc cgcttctgta ttgccgggag accggcaagc
      301 gctttggggt ccttataacg gatctccggc tttttgcgga taacgcccat gccggagcac
      361 ggaacgtccg caatgacaag atcgaagctc ccacggagcg cttcatacgg cttggaagca
      421 tccatcggcg cagtgcggat gatggaaaaa ccgagtctct cggcgttttc ctctacaagc
      481 cggagctttt tttcgtgaag atcacaggcg agaatttctc cgtcgttttc catcagcgac
      541 gcgcagagaa ggctctttcc gccgggcgcg gagcaggcat ccagcacgcg catccctttt
      601 ttcggcgcgg cgcacaaaac actcatcgct gcggcagcat cctgcacaaa gaactcgccc
      661 gcagcaaagc cggggagcgc ggcagcgctg cctttatcct ccacgcacac ggacacggag
      721 gaaagctcgc tgacatgcgc gcgaacgccc gccgaggaaa aacgctccac aagtgacggc
      781 gcgtcgccgt accgggtgtt caccgagact gtgagctgcg gcgcgcggtt acttgccgcg
      841 aaaaaggcgt gcgccccgtc atagccaagc tcggaaacca tccgctcaca gagccacagt
      901 gggtggctga atcggacgga gagctcctgc accgttccga tgtttgggat ctccgggagg
      961 ttttccctgt tttccgcaac acggcgcagc acggcattca ccagacccgc ggcacgggag
     1021 ccggagcgtt tcgtctgttc gaccgcttcg ttgaccgccg ccgacacggg tattttatcc
     1081 ataaacagaa gctggcagac accgagccgg agaatatcca gaaggttcgg ctcgagcttt
     1141 gcagcaggaa cgctggaata gcagccgatg taataatcac agagcgccgc gttctgcagc
     1201 acgtccagac agagctttgt gcagagcgcc gtgtcgcgct catccagaga aaacttttcc
     1261 gccgccgtct ggatcgtctg ctgcgaccag gnnnnnnnnn naggacatac agcgccgcct
     1321 cgcgggcgcc cgccgtgccg ttagccatca gccttcatcg ggtggccgag cagaaaagcc
     1381 gctgcgctca tgcgcttctt ccctgccgcc tgtatctcgg tgatgcgaag agtctggccg
     1441 ccgccgcagg cgacttcaat gcccgctttt ccggcggata cgatctttcc gggcgcaagc
     1501 gctgttttcg tatcggtgta ttccgcgccg aacacgcgaa acgatacgcc gccgagctcc
     1561 gccgtggcca ccggccacgg atccagaccg cagatgtgct tcacgatctc acgcggcgat
     1621 ttgttccagt cgatcggaca catttcctta tggagcattt tcgtgtaggt ggcgtccgcc
     1681 tcgttctgcg gtacgcggac aacatcaccg gaagcgatct tccgcacggt ctccacaagg
     1741 agctctgcgc cgaccgtttt gagccggtca aagagctccc cggacgtttc aaactcgccg
     1801 atctccgttt cgcgcgaagc gatcacgtcc ccggcgtcca tcgccgcagc catgtactgc
     1861 acggaaacgc cggtcgtctt gtccccgttg agcaccgccc attgaatggg cgcagcgtcg
     1921 cggtatttcg gcaggagcga gccatggaca ttgatgctgc cgtattttgc cacgtcaaga
     1981 aacgtctgcg gcagaatacg gccgtaagcc acgaccacaa gaagctccgg cgcgagcgtg
     2041 cgcagaagct ccgttgcggc gccgtccttg aaggttgcgg gctgatatac gggaataccg
     2101 cgctccacgg ccagcgtttt caccggagaa aagctctcct gcatgccgcg gccccgcggc
     2161 ttgtccggct gcgtgaaaac cgcggcaacg tcctcaccgg cctcgagaag cgcgcgcagc
     2221 gattcttccg caaagtccgg cgtacccata aaaacaattc tcattcttta tcgtctccgc
     2281 cgtagagctc ttccatttcc tcgtctgtgt acatatgcgt cgcgcggtcg gtaaagagaa
     2341 cgccgtcgag atggtcgatc tcgtggcaga agcagcgggc ggtaaggccg gtgccttcga
     2401 cctcaaacgt gttgccgttc cgatcctgtg cgcggacttt gacatggttc ggacgcttga
     2461 cgacgccgta ccgctccggc aggctcagac agccctcgcc gccctcctgc tcgccgtccc
     2521 gttctatgat ctcggggttc acgagctcga taaaatactc gtcctcccct tccgggacgt
     2581 tggtttccat cacaatgcag gcgcggcgga taatgccgac ctgcggcgcg gcgaggccga
     2641 caccgttcgc ctgcgaaagc gtctccttca tatcgtccag cagctgatgc agccgctcgt
     2701 cgaaggctgt atacggacgg cagaccttcc gcagcgcagg gttttcctca gtcagaatat
     2761 ttctaagcgc catagtattc actccattgg gttatagtcg gcaaagaccg acacaccctt
     2821 gtattctttt gcggtattgc attgaatgag aagaccggac acaagcgtcc ggatctcctt
     2881 gtcaaaccgg caatgcagcg ttacacggta gcggtagcgg ttgctgacgc gcacgaccgg
     2941 cagcggcgcg gggccgagca cagcggcgtc ctttcgtccg cgaagctccg atcttatccg
     3001 gtcgcggatg taggtgcagc accggaggac ggcagattca tcctccgccg acaccgtgac
     3061 ggtcacgata tccgaaaacg gcgggcagtt ctgcagccgc cgcagctcga tctcttcggc
     3121 ataaaagccc tcgtagtcct gccgggacgc cataccgatc acgggattct gcggcgtgaa
     3181 cgtctggatg atcgcccgtc cgggcttcgc gccgcgtccc gagcgaccga cgacctgcgt
     3241 gatcagagaa aagcagcgct ctcccgcgcg gtaatcgccg caatacagga gctggtcggc
     3301 ggaaataacg ccgacgagcg tcacgtcctc aaaatccaga ccctttgtga ccatttgcgt
     3361 gccgatgagg atcggcacct tcttctcccg aaactgggag aggatcttct cgtgcgaacc
     3421 cgcaggggaa acggaatccg catccatacg cagtatctcc acatccggca gtgcctcgcg
     3481 cagatgctcc tcgagcttct gcgtgccggt accgaactgg ttgagcaagc cgccgcattc
     3541 ggggcaggca tcgtcgagcg ctcgcgtata gccgcagtga tggcagacga gccgccgggt
     3601 gacgctgtgc cacgtgagat tcacgctgca atgcggacag ctgtaggtat agccgcactc
     3661 accgcaggcg acaaggcttg ccgcgccgcg gcggttcaaa aacaggatgc tctgctcgcc
     3721 gcgggaaatg ttttcccgca gttcgtgcag cagcacaccg cttacttcgc tgccgtttcc
     3781 gccgcgaagc tcctgccgca tatctacgat gcggacggat ggcagctgac gggcgttgaa
     3841 gcggtttttc agggtaaaca gatgatacac gcccttttcg gcgcggtaac ggctttccac
     3901 atccggcgtc gcgctgccga gaaccagaag tgcgttggaa gatgcgcacc ggaacttggc
     3961 ggcgtcgcgg gcgtggtagc gcggggagtt ttcggatttg taagtatact cctgctcctc
     4021 atccatgatg atgagcccaa gatcgctgca cggggcgaag acggcgcttc tcgttccgat
     4081 cacaacgcgc gctcttccgg tcttgatgcg cttccactca tcgtagcgct cgccaacggc
     4141 gagagagctg tgcagaaccg cgatatcatc tccgaagtat gaggagaaaa tgcggatcat
     4201 ctgcggcgtg agcgcgatct ccggcacgag caggatggac gccttccctt ttcggcgcat
     4261 ggcgtcgatc agatggacgt acacggatgt cttgccgctt ccggtgacgc catagaggag
     4321 cgacgccgat gcatgctcgc cgtcgtacag cgataggatg ccctcaaacg ccgcgcgctg
     4381 ctcctcgttg agctccggca gcggggattg ctcctcactg cggagaacgg agcggcggaa
     4441 cacctcgtct tgcgtgatct ccgccagtcc cgcttttacc agcgcattga cggacgagga
     4501 cgaagcgcct gtgaaatagc agagctcgga aaaggaaacc cgatccagcg tacacaaaag
     4561 acgcagacac gcgctttgca gcggcgcgcg cttctcgcgg cggtccgccg cgacctgcgc
     4621 gtcctccgcc gaaacagtca gagagaccat gcggatcttt ttatcgccga cgcggcgctt
     4681 ttcgcggctg tccgtttcca gaacgccctt gcgcacaagc gactgcagcg cccgtgccgg
     4741 gctcacattc tcaaaaagcc gctccagatc gaccagcgga cagcttccgc catgggcaaa
     4801 aacggcatcg agaacaagct gctcctgcgc agactttccc gctgcggcat acgcgctctc
     4861 ccggtcaaaa ccggcgcaga gcgtatatac cgactccatc tggtaccaga gtccggcggg
     4921 cagcatcgca tgtacggcat cgtataccgt gcagaaatac cgctcgcgca gccagaccgc
     4981 caagcggatc atctccggag agagcacggg cgacgcgtcc agcaccgagg caatgctttt
     5041 cagccgcata ccggactgcg ccgtaccgag agacagaacg atcccctccg tgcggcggtt
     5101 gccgccgccg aacgggacga cgacacgcac gcccggcacg accttatctg cgagcgcggg
     5161 cggaatgcgg tagtcaaacg gtcggtctac ccagtatgtc gcggcggcga cagcgatttt
     5221 tgccgttgcc ggcgaatcag tcatccgtct tttccagcgt cagtttgccg gaatacactt
     5281 cgtnnnnnnn nnnctgccga ccttatcaac aaggctcgcc atcgggggat aaagcatcat
     5341 aacagattcc attcctttct gatccggcgt ttaccggtgc aaaacattgt atgcgattta
     5401 tttgtcgagg atggctccga gctcgcggga tgcgcggtcc gggtcatcgt tgatcacagt
     5461 ataatcatag cttggagcca gcgccatctc gcgccgtgct gtgtcgagcc gcgcacggat
     5521 cgtctcttcc gattccgtcc cgcggccgcg gagacggcgt tccagcgcat ccagccctgg
     5581 aggagcaagg aacacggtaa cggcttcagg cagcttctgc ttcacctgcg ccgctccctg
     5641 gacctcaata tccagaatca cggacacgcc gctttccagc cgcgcacaga cctgcgactt
     5701 cggcgtaccg tagcaattgc cgacaaactc ggcatgctct aacagctcgt catttcggag
     5761 catttcctca aagcgctctc gggaaacgaa aaagtaatcg acgccctctc tctctccggg
     5821 gcgcgggcgg cgtgttgtgg cggatacgga gaactccaca ttctcccgcc ccgccatcat
     5881 ctttccgatc acggtggatt ttccgcagcc ggacggcccg gagatcacca gcagcttacc
     5941 gcgtttcatt cctcgtccct ctcttccgcc aaaggctctt cgctgcggcc gatcatgcgg
     6001 gcgctgaccg tttcaggctg gagcgccgag aggatcacat gcccgctgtc catgatgatg
     6061 actgcgcgcg tgcggcggcc ataggtcgcg tcgatcagct cgccgcgctc gcggacatcc
     6121 tgaacgatgc gcttgatcgg cgcggattcc gggctgacga tagcgatcag acgcgaggcg
     6181 gaaacgagat tgccgaaacc gatgttaatg agcttcatat ccgcacctca ttccacgttc
     6241 tgcagctgct cgcggatctt ctcgatctcg gatttgagat cgaccacgac cttggcgaga
     6301 gatgcgtcgc tgcacttgga gccgatggtg ttggattcgc ggttgaattc ctgcacaagg
     6361 aagtccatct tccggccgat cggagagcct tcctccagca tggtgcggaa ctgcgcaatg
     6421 tggctgcgga gccggaccgt ctcctcgtcc acggcggtgc ggtcggcgaa gatcgcagct
     6481 tcggtgatga cacgctgctc atcgagcgag cggtcggcca gaatgtcgcg cagacgcgct
     6541 tcgagacgct cgcggtattc cttcaccgtc tgcggggatc gctcctccac aacggaaaca
     6601 agcccctcaa tggtttcgag ctttccgagc atgtcccggc gcagccgttc gccttcgcgc
     6661 tcgcgcatgg cgttgaattc ctccacggct tttgccgtga tctccgagag cgccgcggcg
     6721 gctttgtctt tatccagctc gcgccgctcg accgtgagca catccgggaa ccgggcaagg
     6781 ctgaatgcat tgagtccgct ttcgagtccg agcgtttcgg cgatgcgcgc gaccgcgtcg
     6841 cggtagccgc gggcaagttc ctcattcatc gaaacgaccg tcccggaatc ctgcgaagcc
     6901 tgcgccgata caaaaacatc caccttgccg cgcgaaacgc cggcggagac cgcctgcttt
     6961 accgtatcat cggcaaaaag gaaattacgc ggcagccgga ccgaacagtc cagatagcgg
     7021 ttgttcacgc ttttcagctc aacggtgatc tcctgaccct ctacggagcc cttggcgctt
     7081 ccgtaaccgg tcatactgag cagttttccc atttcttccc atctccccaa atatgtgaat
     7141 caaaaagtta tcaacagcag caaataaacg gcgcgcagcc gccggaggtg gccatcagcg
     7201 ccatgcatgg gcagaacgcg ccgcagtcga gcgtgttcat tccaacggcg cttccgcccg
     7261 caaacgggcc gttcgcctga ctttccattt gagcgaccgc ctgacggtat tccgc
//
LOCUS       DAWAAE010000104         7284 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4496_length_7284_cov_3.001522, whole genome shotgun sequence.
ACCESSION   DAWAAE010000104 DAWAAE010000000
VERSION     DAWAAE010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_7284_cov_3.001522"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1573 a         2131 c         2362 g         1218 t
ORIGIN      
        1 gcttttacgc agcgggcgcg ggcatatgag cgaggatacc gtgaaggcgg caaaacttgt
       61 gaaggacgct ggtttcacgc tgattctgca gatgatgacc ggccttcccg gcagcgacga
      121 tacgcgcgat atccagacgg cgcgctctat tgccgcgctt cacccggacg gcgtgcgcat
      181 ttatccgacg gtcattctcc gcgatacgcc gctctgtgac ctctggcagg cggggaagta
      241 ccgggagcat accgtggagg atgccgtgcg cgtatgcgcg cgtattctgc ccatatttga
      301 gaatgccggg atcccggtca tccgtctggg gctgaacccc tcggacgagc tctccggcgg
      361 cgccgccgtc ggcggagcgt atcatcccgc gcttggcgag ctggtgcgtt cgcggctctg
      421 gcgcgataag gccgaggcga tcctctccgg catcgaaccg ggagcggacg tcgttctcgg
      481 cgtttcggcg aaccgtattt ccgtgatgac agggcagcat cgcgctaatc tatcgtatct
      541 tcaggagcga ttcgcgctcc ggtcggtgcg cgtttgcgcg tccgacgtac cggacgggga
      601 gatcgtccgg atataagttg caaaaaaaga cgatttagta tataataaat tgttttgatg
      661 catctttaat tgctgaggag aagaaaccgt gtatttaaaa gcgctggaac tccagggctt
      721 caagtcgttt cccgaaaaga cgcggctgag ctttgaaaaa gatataaccg tgatcgtcgg
      781 acccaacggc agcggcaaat ccaatttgtc ggacgcgctg ctgtgggtca tgggcgagca
      841 gaaaacgcgg atgctgcgcg gcgggaaaat ggaggacgtc attttcggcg gcacagaaaa
      901 gcgcggcgcc atgggctttg cgcaggtgac gctcgtgctg gataatacga cgcgcatcct
      961 gccgctcgat accgacgagg tcagcatcgc ccggcgctac taccgcagcg gcgagagcga
     1021 atattacatc aaccgcgagc aggtgcgtct gcgtgacgta acggaactgc tgatggatac
     1081 cggtctcggc tgcgacggct actcgatcat cgggcagggg cgtatcagcg agatcgtctc
     1141 cgccaaatct accgaccggc gcgagatatt tgaagaagcc gcgggcattg cccgcttccg
     1201 ccaccgcaag gacgagtccg agcgtaagct cgcccgcacg gaggacaacc tcctgcgcat
     1261 cggcgataag atctcggagc ttgaaataca ggtcgaaccg ctgcgcaagc agtcggacac
     1321 ggcgcagaag taccttgttc tgcgcgacca gctccgcgaa caggaggtat cgctctggat
     1381 ggcgaacctc gatcggctgc acgagcagtc ggagctgctg cgctcggact atgagcgctc
     1441 ggtcgtggag tgtacggcgg cacgctcgga gcaggaccgt ttgtatgccg agagtgagcg
     1501 ccttgctgcg cgcatgcgcg aaatggacgt gaagtctgag gatcttcggg aaaagcttcg
     1561 cgcgacggag gctgcggctg cagacgccga gagcggctcc gccgtggtgc gcacgcagct
     1621 tgcgaacaac gctgaaacgc ttttgcgtct gcagcgcgag atcggcgagc agaaggagcg
     1681 cgccggaggg ctatcccgtc aggtcgggga gcaccgcgcg cgcatcgaga gcatcgccgc
     1741 cgaacggcgc gcgctggccg accgcgcgga ggaactccgc cgcagcgagg cggaaaacgc
     1801 ctccggcatg gacgaacaga acaagcgtct ctccgccatg ctcgcgcatg agagtgagct
     1861 caccggtacg cttaccgaga gccgcacggc aatcacgctt cttgcggaaa accgcgagac
     1921 gatggaagag cgcgctgtgc aggcaaagga agagctcgcc gccgcggagc ggaaggacga
     1981 ggaagcctcg gcgcgtctca cggaagagaa gaagcgcctg aatgagataa aggaaaaaga
     2041 acaaagcctt gccaacgtga tctccggtca ctccatgctc atggcagggc gtgagcagaa
     2101 ggcggcggag accgccgaac ggcagacaaa gctcaccgtg gagctgcgct cctgcgattc
     2161 gcgcatccac ctcctttcgg aactggaaaa ggagctggag ggcttcagca aggccgcgaa
     2221 gatcgtcatg caggagtcgg agcgcggcac gctgcgcggt gtgcacgggc cggtcggcaa
     2281 aatgatccgt accgaggacc ggtacgcgct cgccgtggaa acggcgctcg gcgcgcacgt
     2341 cggcgatatt attgttgata cgcaggataa cggcaaggat atattcgcgc tcttgaaacg
     2401 gcgcgacggc gggcgcgtaa cgctccagcc gatggatgtg atccgtccgg cgcggctgaa
     2461 cggtgagccg agaggcgagg cgggctacgt cggcgtgtgc tccgatcttg taaactgcga
     2521 taaacgctac agcgccgtca tagcgagcct tctcgggcgc acggccgttg cggaaacgct
     2581 caccgacgcc atcgccattg cacgccggca caacaacagt ttccgcatcg tgacgctcga
     2641 cggacagatt ttgaatgccg gcggctcgat gaccggcggc agcgccggga aaaacaccgg
     2701 catcctctcg cgcgccaacg agctcaagcg tctccgcgaa aaacgcggcg atctggaagc
     2761 gcagctctcg cagacggaat cgcagcggcg ggaagccgag cgcgagctgc agagcgcgcg
     2821 ctacgatctt gaaacggccc gcgaagaaca cgctgcggcg cggcaggaaa cggcgcggca
     2881 ggagggcctc gttgcgcagg cgcagcttct gcgcacggca gcggcggatg cgctcgacgc
     2941 gctcgacgag gagatccgct ctgcctccgc gcgacggcgc gaaaacgaga accgcacggc
     3001 ggcactgcag gaaacgtgcg ctgcggcgga gaaggagctt gcctcgctcc gcgcggacat
     3061 ggaaaaagaa tccgccggca tcgagagctt tgaagcgcgc cggcgggagc tcgaggagag
     3121 tttcagcgcc attcgcgcgc agcaggcgtc gcttgacgcc gagcgcagca ctgtgcttct
     3181 cgccatcgaa cagctcgaac gcctgcgcgc cgagttggag agcgattccg gcgcgcgccg
     3241 cagcgctatg agcgccgtgg agcagcagaa agcggagctc gaagagcagc agactacgct
     3301 cgcggagcgt ttggaaacgc tgcgtgcaca ggcgcagcag gaaaaggacg cgctcacgca
     3361 gctcaccgca aataaaatgg agctggaagg ccgccgcagc gccgctgaca aagccgcgca
     3421 ggagaagaac cgcgccctgc tcgatctcga acgcagcagc gccctgctcg aacagaagaa
     3481 aatggcggcg gagatggaag agcgccagat cgtggaaaaa ctctgggaca gctacgagct
     3541 cagccgcacg gaagcggaaa aagtccgtca gcccgtcgaa agccttgcca aggccggacg
     3601 gcaggtcagc gaactgcgcc ggagcatgtc cgcactcggt acgccgaacc tcggcgcgat
     3661 cgaggaatat aagcgcgtga gcgagcggta caccttcctt tccgagcagc gcgacgatgt
     3721 gcagaaatcc aaggatgaga ttttgaagat catcgccgat atcaccgcgc agatggaaga
     3781 gatctttgtc cgcgagatcc aggagatcga cgaggcgttc cgccagatat ttgtagagct
     3841 cttcggcggc ggcaaggcgt ctctctcgct ggaggatatg aacgatgtgc tcaactgcgg
     3901 catcgatatc cgcatccagc cgcccggaaa agcggtttcc aacatcagcc tgctctccgg
     3961 cggcgaaaag gcgttcgttg ccatcgcgct gtattttgcc atcatgcgtg tccgcccgac
     4021 gccgttctgc attatggacg agatcgaatc ggcgcttgac gaggaaaacg tcgcccggta
     4081 cgcggcgtat atgcgccgca tgtgcggaaa aacgcagttc attgccatta cgcaccgccg
     4141 cggcacgatg gaagaggccg atcagctcta cggcgtcacc atgcaggaaa aaggcgtcag
     4201 caccgtgctg gcgatggata tggaagaagc gctccaaacc attcgctgaa aggagaaaac
     4261 agccatggga ttctttgata aaatgaaagc cgcgttcaac aacgcaatgg cggacttcac
     4321 cggagaaaac gaggaagcct acgaaacact ggaggaaatg ctcattctgt ccgacgccgg
     4381 tatggatacg acggaggaca ttatggaaaa gctgcgccgc cgcgtccgcg acgagggctg
     4441 ccgcggcgag gagcagctcc gcaaaatgct cgcgtccatt ctgaccgata cgatggacgc
     4501 cggggataac gcactgcatc tcgacacaaa accgtccgtc attctcgttg tcggcgtcaa
     4561 cggcgtcggc aaaacgacga ccatcggcaa actcgcctcc cggctgacgg cggaaggaag
     4621 atccgttctt ctctgcgcgg gcgacacgtt ccgcgccgcc gctgccgaac agctcaccgt
     4681 gtgggcagat cgcgcgggat gcgatatcgt gcgtcacgaa gaggggagcg accccgccgc
     4741 cgtcgtgttc gacgcgatcg ccgcggccaa ggcgcgcggc cgcgacgtca tcatcgtgga
     4801 taccgccgga cggctgcaca ataaggccaa tcttatgaac gagctctcca agatccgccg
     4861 cgtcatcgac cgcgagctgc cggatgcgag cgtggaatgc ctgatggttc ttgacgctac
     4921 gaccggtcag aacggtctca tccaggcgaa ggtcttcggc gagtcggcgg ggctcaccgg
     4981 catcgtcctc acgaagctcg acggcaccgc caagggcggc atcgtgttcg ccatcaagcg
     5041 ggagctcggt ctgcccgtga agttcatcgg cgtgggcgag ggcgtgaacg atctcaagcc
     5101 ctttgtaccg gaagaatttg cggaggcgct gctcgaatga agctggtttt ggcatccgac
     5161 aataagaaca agctgcggga gttccgcacg ctttttgaaa acttcggcgt agagcttata
     5221 agcaaagccg agtccggctt tacggaagaa gtcgaggaga ccggcgaaac gtttgcggaa
     5281 aacgcccgca tcaaggccga agccgtcatg cgcgccacgg ggcttgccgc catcgcggac
     5341 gattccggcc tctgcgtgga cgcgctcggc ggcgcgccgg gcgtgcattc cgcgcgctat
     5401 accggaaatc atgacgattc cgacgccgac cgctataacc tcctgcttcg gaatctcggc
     5461 gggagagacg accgcgcggc gcggtttgtc tgctcgctct gctgcgcttt cccgaacggc
     5521 gacgtactta ctgccgaggg gacgtgcgag ggaacgatcc tttctgcgcc ccgcggcgag
     5581 aacggcttcg gctatgaccc ggttttccgc ccggacggct ttgaccgctc catggcggag
     5641 ctgacgatgg aggaaaaaaa cgcgatctcc catcggggga aagcgctggc gcaattcaga
     5701 cagaaatggg agaaatacta tgatcaccag taaacagaga gcgcagctgc gcggcatggc
     5761 ggcggcgctg aataccgagc tgcagatcgg caagagcggc atcaccgaaa acgttgtccg
     5821 tcaggcggag gaagcgctcg ccgcccgcga gcttatcaag ggccgtgtgc ttgataactc
     5881 cctgctttcg gcgcgggagg cgtgcgacgc gctgtgtgag gcgtgcggcg ccgacggcgt
     5941 gcaggtcatc ggcagcaaat ttgttctttt caaacagaac gaaaaggacc ccaagatcgt
     6001 cctaataaac gatcgcaaag gaaagaagta acatctatgc gtcttttgat ctacggcggg
     6061 acgttcaatc cgccgcatct cggccacatt gacgcgctct cctgcgccgc ggcgtctctc
     6121 agcgcggatc gatgcattgt cgtcccggca ggcatcccgc cgcacaaggc gcttgcggag
     6181 ggcagcccga cggcggagga acggctccgg ctgtgcgagc tggcgtttcc ggacgcggac
     6241 gtcacgccca tggagctgcg gcgcgaaggg aagagctaca ccgtggatac actgcgggag
     6301 atatcccgcg gcaacccggg ggcggagcta tattttctcg tcggaacgga catgcttctc
     6361 tatatggagc agtggtatga gtttcgctct ctcttttcgc tctgcacgct cgcggcgctt
     6421 cctcgtgccg acggtgatct ggcggagatc gaacggtatg cggcgtatct gcgcgaaacg
     6481 tacggcgcgc ggatcgagat catagcgaaa acgcctctgc cgatggattc tacggcgctt
     6541 cgcgcggcac tgccgcagcg cggcggtacg gaacggctct gcgacgccgt gtattccgag
     6601 atcatccgct gccggctgta cggtgccaaa ccggatcttg catggctgcg gggaaagacg
     6661 gacgcttacc tcaaacccac gcgcataccg catgtgcgcg gctgcgagga gacggcggcg
     6721 cggctggcgc tgcgctgggg cgaagacccg gaggatgccg ccgaggcggg tattttgcac
     6781 gacattacca aaaggctgtc ggacgacgag cagttgcgtc tggcggaaaa atatggtatt
     6841 gtgttgaatg ctgccgatat cgccgcgcca aaaacgctgc atgccctcac cggtgcatgg
     6901 ttcgcccgtg agcatttcgg cgtgccggac aacatttttt ccgcgatcga atggcacacc
     6961 acaggccgtg ccggaatggc agcactcgag aaaatcgtct accttgcgga ttttatcgag
     7021 ccgacacgcg actttcccgg tgtacaggat gtccgaacgc ttgcttttgc agatctgaac
     7081 gccgccatga tccgagcgct gcaaatgagt atggacgagg tcaaacgccg cggcgcttcg
     7141 ccgcatccaa ggtcggcgga ggctctccgc tggctgaaaa cacagaattg agaagaaagc
     7201 tatgagtaat ttttcccaca aaggcggcgc tcacgaaaac gccgcaaaaa agaaagagac
     7261 aaaagaggag agcttcgccc atcg
//
LOCUS       DAWAAE010000105         7221 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4539_length_7221_cov_3.509768, whole genome shotgun sequence.
ACCESSION   DAWAAE010000105 DAWAAE010000000
VERSION     DAWAAE010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_7221_cov_3.509768"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4452..4461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5722..5731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1218 a         2291 c         2432 g         1260 t
ORIGIN      
        1 acgacctgac ccagatgacc ttcggcttca gccgtgacga cgccggcaag ttcctcggcg
       61 cgtactacga caagaagatc tacgagaacg atccgttcgc ccgcctcgac caggtcggcg
      121 tcggcaagct ggtcaagatg gccgccactc tcggccgcca gacccgcccc gacctgcacc
      181 tcggcatctg cggcgagcac ggcggcgatc cttcctccgt cgagttctgc cacaaggtcg
      241 gcctggacta cgtgtcctgc agcccgttcc gcgtgcccat cgcccgcctc gccgccgctc
      301 aggctgcgat tcgcgatcag cactaagcag agtttcagcg ctgcataaag caaagataaa
      361 ccaacaaacg gccacatcac ccaagcggtg atgtggccgt tattttttgc cccgaaaggg
      421 acaaaaaggc tggttatcct gttgaagtga ccccaaaaag ttagacaaat aaattaaacg
      481 cgaattgttc aagctgccga aagggcttgc tgtcttttat ttattccgcg tcgaggtcgg
      541 cggcggcgac gaggttcgag ccggtgtccg cgatgttctc gcagagaata tcgccgatgt
      601 gtaccggcgc cttgacctcc agcgcgcgga cggcgtccat cacggcaaag atcttgccct
      661 tcggcacgtc cggcacggtc ttcaccgcga cgaccgtgcg cacgccgcca agagcgcgca
      721 cggtgctcgt cacggtgcgc acaggtgccg tggcctcgct cttcgcgtac tccgccccgc
      781 gcgggcaggt attgcccgta acggagacaa cctgtccgtt ttcgatttcc acgtggatgg
      841 cgcagcccat cgggcagcgg atgcatgtca ggtccatgat ttattcctcc tccacacaga
      901 atacgacttc ctgcgcgctc tcgccgagag cttcgcgcgt gagcgtgaac gactccattt
      961 cgccgggcgc catgatctgc tttttgcggt gggaaatgcg ctcgccgtcc acatagcagg
     1021 agagatactt gccgtaatac ggattcgcca cgcggaagcg gacgacgacg ctctcgccca
     1081 tcgcggcggg gcggacggtc tgcggcacgg tgtagcgcac gccgtcgcgc ccggtgagct
     1141 tcactacgcg cgcactcttc ggcgtgccgc cgcggacgaa cttcgccgcg gctctcccgg
     1201 cgcgcgcggc ctcttcggac acatagtcca cgagatcatg gacgtgcagc acattgccgc
     1261 aggcaaaaat gccgggcacg ttcgtttcca gcgattcgtt caccgccggg ccgccggtca
     1321 cgcggtcgag cgtcacctcc gcgccctcgg aaagctcgtt ttccggcaga aggccgacgg
     1381 agagcagaag cgtgtcgcac gggatatact tctccgaacc cggcaccggc ttgcggtcgg
     1441 ggccgacctc ggcgagcgtg acgccctcga gccgctccct gccgtggata tccacaacgg
     1501 tgtggctcag aaggagcggg atatcgtagt cgtgcaggca ctgcacgatg ttgcgcttga
     1561 gtccgccgga gtacggcaga agctcggcca cgcacttgac gtgcgcgccc tcaagcgtca
     1621 tgcggcgcgc catgatgaga ccgatgtcgc cgctgccgag gatgacgacc tcgcgtccgg
     1681 gcatgaagcc ctcgagattt acgagccgct gcgccgtacc ggcggagtag atgcccgccg
     1741 gacggtagcc gggaatgttc agcgccccgc ggggccgctc gcggcagccc atcgcaagaa
     1801 tgacggcgcg gccgcggagc tgctccacgc cgcgttcgcg gctcgtaacg gtaagcacgc
     1861 gctcgttcgt gagcgagagc accatcgtcg aaagacggat ggcaacgccc agctcctcgg
     1921 cgcggcgcag aaaccgcgcg gcgtactcgg ggccggtcag ctcctcgcgg aacgtgtgca
     1981 gaccgaagcc gttgtggatg cactggttca aaatgccgcc ggggacgctg tcgcgctcga
     2041 tgacgagcac atcgtccacg cccgcttcct tcgcggcgat ggcggcggcg agaccggcag
     2101 gaccggcgcc gatgataatg atatcgtagg tattcatgcg ttttcgccgt cctttctcgt
     2161 tttgccgacg attgggaacg atgcgccgcc gctcttcgtg agctgctcct gcggcacgcc
     2221 gagctcacgc gagaggatct ccagcacgcg cggcgagcag aagcccgcct gacagcggcc
     2281 cataccggcg cgggtacggc gcttcacccc gtcaagactg cgcgcgccgg gaacgcggtg
     2341 gatggcgtct acgatctcgc cctcggtcac ctgctcgcag cggcagacga tattgccgta
     2401 cagcgggtgc tccgcgacga acgcggcgcg ctcctccacg gtcatctcgt tcgggcggca
     2461 gacctccgga cgggtggaat cgtatttctt gtttgccggg gcggaaacga gatatgccgc
     2521 gctctcggcg agaaacgcgc cgatggcggg cgcggcggag agaccgggcg actcaatgcc
     2581 gacggcgttc aaaaagcccg gcgcgccttc ggcctcgccg ataatgaaat cgtgctcgtc
     2641 cttctccaca tgcgcgcgaa ggccggcgaa gctcgtgatc gtgcggttgc cgggaagcga
     2701 ggggacgctc tttctcgccc gctgcatgac gctctcgaga cccgcggcgg tcgtgttcgt
     2761 cgctccggga tcttccacat ccacggcggt cgggcccatg aggagattgc cgtggacggt
     2821 cggcgtcacg agaacgccct tgcccatttt ggtgggcagc tggaacacgg tgcgcgagag
     2881 catgccgccg acctttttgt ccagcagcat atattcgccc ttgcgcggcg tgatcgtgat
     2941 ctttttatcg gacaccatgt tgttgagctc ggcgctgtgc agtcccgcgg cgttgatgac
     3001 gaccggggcc tcaaagctcc ccttcgcggt ctcaagcttc cagagcccgc cctcgcgggc
     3061 aatgctctcc acgcgggtat cgaagaagaa ctccacgccg ttgcgcacgg cgttttccgc
     3121 catggcgcag gtgagctcaa aggggcagac gatgccgctc gtgggcgcat agagcgcggc
     3181 gacggcctca ggggagacgt tcggctcaag ctcgtgcagc tcctcgcgct ccacgatgcg
     3241 aagaccgtgc acgccgtttt tcacgccgcg gagcaaaagc tcctcgagtc ccgggcggtc
     3301 ggcctcgtta aagcacagca cgagcgcccc gcagcggcgg aacagaattt gcagccgctc
     3361 gcagagcttc ggcatcgcgt ggcttccctc cacgttgaac cgtgccatga gcgagccggg
     3421 gcgggcgtca aagcccgcgt ggacaatggc ggagttagct ttggacgtgc cggtgcatac
     3481 atcctcgccc gcttcgatca cggcggcgcg cagcgagtat ttgcccagct ccatcgccgt
     3541 ggcgcagccg acaacgccgg cgcccacgac aaggacatcg tattgcatag cctttccctc
     3601 tctttatcgc ccggcgcacg gctcgcgcgg ggcggttttt ttattggtta tccagcggcg
     3661 ggccgagccg cgcgagcaca gccgtaaaat attccggcag cggcgacgtg agctccatgt
     3721 gctctccggt gcgcgggtgg ataaatttga gcgtccgcgc gtggagacac tgcccttcca
     3781 gacccttttc cggctttccc gcgccgtaga ccatgtcgcc gagcagcggg tggccgatgt
     3841 gcgccatgtg gacgcggatc tggtgcgtgc gcccggtctc gagcttgcag cggatgtgcg
     3901 taaagccggc gtaccgctcg atgacctccc aatgcgtcac ggcgtggcgg gagtttttct
     3961 ccgtaacggc catgcgcttt cgatcgaccg ggtggcggcc gatgggggcg ttcaccgtgc
     4021 cggaatcggc gcggaagccg ccgtggacca cggcctcgta cacgcgcgaa aggcttcggt
     4081 cggcgagctg tgccgagagc gactgatggg cgaaatcgtt ttttgccacg accagaaggc
     4141 cgctcgtgtc gcggtcgatg cggtggacga tgccggggcg catctctccg ccgatgccgg
     4201 agagcgaatc gccgcaatgg tgcagcaggg cgttgacgag cgtcccgtcc gggtggccgg
     4261 gcgcgggatg cacaacgaga ccgcgcggtt tgttcaccac gatgagatcc gcgtcctcgt
     4321 aaacaatgtc gagcgggata tcctgcgccg cggcgctcgc cggggcgggc gtatcatcct
     4381 cggcgacgag aaacacgtcc cccgcgcggg tgagctcgct tttcttgacg gattttccct
     4441 ctcttgtcac cnnnnnnnnn ngcggcgatg agccgcgcgg cggcggagcg ggtgtagccc
     4501 gaacgcgcaa ggagagcgtc gatcctctcg ccgctctcct gcgcctgtac ggttgtgatt
     4561 ttcatttgaa ctccgcgaga atggagtcga gatcaaactc ttccgccgaa gcagccttcg
     4621 gcgcttcctt cgcgggagcg ggggcagcct tcggcgtctc tcttatggga gcgggagctg
     4681 ccgccgtcgg ggcggccttg ggcgcggcgg gtctcgcggc ggccggacgc gccacggtgc
     4741 gcaccgtggc ttcgttctgc gcggcaagat cggcgcgcgt taccggcgcg gcgggaaccg
     4801 ccgcgcggcg gacgggctcg gcggcggtgc gcgtcgtttc cgcggtgcgc tgcgccgctg
     4861 cggcgggacg cgctgccgga cggaccggca cggggacgct gttctcgtca atgacgcgct
     4921 tcgtgtgcgg cggtgtcgcc ggaatgccgt ttccggcggc gcgctgacgc gccggagagg
     4981 gcgcggcggt gccgcgggcc gaaggcgctg cggtcgtggg ggcggcggga cgcggcgcag
     5041 tgcggcgcac gggagcttcc tccgcggtgc gcggtgcgcg gcgggcggcc ggctcgtcgt
     5101 catactcgcg gcggcgggca ggacggacgg gcgcttcttc ctcttcatag tcgtcaaact
     5161 catccgcgcg gcggagtccg ccggtcaaaa tcgctacggc gaagagaatg ccgccgatga
     5221 gcacaagcac atccgccaga ttgaagatgg ggaacttgaa gagctcaagc tcgaaaatgt
     5281 cgggcacata gccgtagatc gcacggtcga tgccgttgcc gaggagaccg gcgagcagca
     5341 gcgcgccgga gaccttcgtg aggccggtgc ggaaatagcc cttgatgaag gcgaacacca
     5401 tgagcgcggt gaacgcgatc agaaggatga gcaagatcca gcgcagcgcg ccgaagctgg
     5461 acatcacgcc gaaggccgcg ccgaagtttt tgatgtaagt gatgtggaga aagccgggga
     5521 tcagcggtcg gatgccggtg ttcagcgcaa cgttggacac cgtccagaac ttgaggaact
     5581 gatcgaccag cagagccaga agagcaatga taccgtaaac catgaatcaa attctccttt
     5641 ctctatggga aagaaattgc ggggagaacg ggggaaaccc ctccgtcacg gctgcgccgt
     5701 gccacctccc ctgttagggg annnnnnnnn ncggggctgt cagccgaagg ctgactgagg
     5761 ggtttgcttg aaagcgtcag cctacaacgc tggcgcatct tgcgcagagc gtcgggtgct
     5821 tttcgttcgt gccgacggtc gcggagcgcg tccagcagcg cgcgcagcgc ggcagaacgc
     5881 tcggctcgac gtggacggtg agcccgtcga agttctcgcc gcggacgcct tccccggcgc
     5941 cgtggacgat cttcagctcg gacacgatgc aaagcggcgc gagatcctcg ctctccagct
     6001 ccgcaaacgg ctcggccgcg gcctcggaca catagagcgt cacgcgcgcg tccagcggct
     6061 tgccgatgag cttttccgcg cgggcaagct cgagcgcctt gttcacgtcg gcgcgcacgc
     6121 gcatgacctt gctccagtgc gcttcctcgc tctcggagag acggagcgcg gcgttcgcct
     6181 ccggcatatc gttgagcgcg acggcgcgcg cgtcctcggc ttcggtgtgc ggcatggcga
     6241 gccagatctc gtcggcggtg aagcagagca ccggcgcgag caggcgggtc atcgcatcga
     6301 gcacctgata aatcgcggtc tgggcgctgc ggcgggaaag gctctcgcgg ctgtcgcagt
     6361 agaggcggtc cttgataacg tccagataga agttcgacat ttccaccacg cagaagttgt
     6421 ggatggcgta ggtggcggtg tggaactcgt agttgtcgta agcggtgcgg acgcgctcga
     6481 tgagatcctg cagacggctg acggcccact tgtcgagcgg cagcatgtcc gcaaaggcga
     6541 cgctctcggc gggatcgaac ccgtcgaggt tgccgagaat gtagcgcgcg gtgttgcgga
     6601 tcttgagata cttatcggag agctgcttga aaatgttgtc cgagcagcgc atgtcctgcg
     6661 tatagtcggc gctcgccgcc cagagacgga tgatgtccgc gccgtacttg gggataatgt
     6721 cgcccggggc gatgccgttg ccgagggatt tatgcatgac gcggccctcg ccgtccacgg
     6781 tccagccgtg ggtgagcacc tcgcggtagg gcgcgtcgcc gcgcaccgca acgccggtca
     6841 gcagcgacga ctggaaccag ccgcggtact gatcgccgcc ctcaagatac acgtccgccg
     6901 gccatacgcc gggcgcgtcg agcagcatgg aggcgatgtg ggtgctgccg gagtcaaacc
     6961 agccgtcgag cgtgtcggtc tccttggtga agccggtgct gccgccgcag tgcgggcagg
     7021 tgaagccgtc cggcaggagc tccttcgcct cggcgtcgaa ccagatgttc gagccgtgct
     7081 cgtcgaagag acgggagacc ttttcgatgg tctccggggt gcacacgggc ttgccgcagt
     7141 cggcgcagta aaacaccggg atgggcagac cccagcggcg ctggcgggag atgcaccagt
     7201 cggcgcgctc gcggatcatg g
//
LOCUS       DAWAAE010000106         7183 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4572_length_7183_cov_3.317200, whole genome shotgun sequence.
ACCESSION   DAWAAE010000106 DAWAAE010000000
VERSION     DAWAAE010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_7183_cov_3.317200"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1375..1384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1482 a         2192 c         2244 g         1255 t
ORIGIN      
        1 cgccgtccgg ctgccgagaa gaacgctctc ggcatggatc acaatgccgg tgccgtccca
       61 cgagagggag gtgacgatct gcatcgagac cggcacgaga ccgagaacgc gctgcgagat
      121 gcatacgcgc atctttccgc cggtcaggcg gatgccgaaa tcgagatcgc cgaaccgctc
      181 gcgcatctgc cgccggagac cgggcagaac gccgctctcg tcggcaagaa cgtaaagatc
      241 gtttttatac agccggacaa cggcgctgcc gtcgtcggca aaggagagct cgccgagctc
      301 ttcgctgcgc ggcgcggcga ggatcgcgtc cacatcgtcc cgaaaggcgg aggcgccgcc
      361 gttgccgatg aggaacgccg cgagcagcag caaaagaagg atccccgcgg gtatcgcgat
      421 cgcccatgcg cgggcgccgc gctttgccgg tttctgcttc atgccatcct gcctcctttc
      481 cgacggatat cgacatactt ttttcagttt aacgcatccc ggcgaaaaaa gcaattgcat
      541 cctgccggag cggaacatat aataggtttt acccgccttc gggcggtaag tttcggaaaa
      601 aaaacggggg taacgcgcat ggctttctct ctggcactcg tggatttcga cggcacgctg
      661 gccgattcga tgccgtactg gctgcgtctg ccggcggata cgctccgcgc ggcggggctg
      721 ccgcagccgg agggctttgc ggaatacatc cgctccgtgc cgatccggga gatcgcgtgg
      781 gacatgggcg caaagtaccc gcagcttgaa gcggaaacgc cgctcgccgt gcgctggagc
      841 gcggagatgg ctgcgcatta cgcgcgggag atccctctca agccgggagc aaagaaactt
      901 ctgcaaacgc tgcgcgcggc ggggctgcgc gtatacatcc tctcggcgac gcgccgcgcg
      961 ctgctcgatc cggccattga gcggctcggc gtcgcgccgc tcgtggacgg cgtttacgcc
     1021 gaggaggaga ccggctcaaa gcgtaccgaa gcgccgtatg cgtttttccg ggacaggctc
     1081 ggcgttccgt atgagaaaat gctcctcgcg gaggacgcgc cgcgcaatct cgccgcggcg
     1141 gaggcgctcg gcgtttcggg cgttggcgta tacgacgaga gcatgaagga atatacggag
     1201 ataatccgcg ccggagcggc ggtgtatctg ccggacttct ccgacctttc ggcgctggag
     1261 gagctgctgc ggtaagcctc ccttgtgcaa agggcagact ctccccggcg gggagagatg
     1321 tcgccgaagg cgacagagag gggggcggag gtggcggacc cgaagggttt gacgnnnnnn
     1381 nnnnctacaa tcccccagtc agcttcgctg ccagccccct ttacacaagg gggccttttc
     1441 tccataaaaa tacgaaaaaa gtattgactt tcctgagata tatggtatta tgtcatggcg
     1501 ctccaaagac agcaggtggc attcgccgta aatcctgccg aggaaagcac gatacttgta
     1561 ttgtgtctcc ccctgtcttt cggcagaggg agtttttctt tcgggcgcag aaatttaccc
     1621 cggaggtgaa accattgcct aacgcgaaag tactcagcga gaaacaggcc atcgtcgagg
     1681 aactcaccaa gcgtatcggt aacgccacgg cgggcgtcct tgtggactac aagggcatca
     1741 ccgttctgga ggataccgct ctgcgccgtg agctgcgtgc tgccggcatt gagtacaccg
     1801 ttgtgaagaa cactctgctc cgcttcgcgg ttgacaagtg caatctcaac gagtttgact
     1861 ccgtgctcaa cggcaccacc tctctggcca ccaccgaggg cgaccccatc gcgcccatcc
     1921 gcatcgtcag cgagtttgcc aagaagatga acggcaagtt cgaggtcaag ggcggcttca
     1981 tggaaggtaa agtccttccg atggccgaca tcgatgagat cgctcagctt cccggcaagg
     2041 atgccctgta ctccaaggtc ctcggcacca tgctcgcacc catcaccagc ctggccgtcg
     2101 ttctcggtca gatcctggag cagaagggcg gctctctcga gaccgccgaa gccgctgccg
     2161 aataatccgg cagacacaaa tcatttttag gaggaaaatt acaaatggct agcgaaaaag
     2221 ttaccgctat gatcgaagaa gtcaaggctc tctccgtgct ggagctggct gagctcgtcc
     2281 acgcccttga ggagaccttc ggcgtttccg ccgctgccgc cgctgccccc gccgccgctg
     2341 ccgccgctgc cccggtcgaa gagaagaccg agtttgacgt tgtcatgacc gagttcggcg
     2401 ctgagaagat caaggtcatc aaggaagtcc gcggcctgct gggtctgggc cttgccgagg
     2461 cgaaggctat cgtcgaagcc gtccccgcca agctgaagga aggcgtctcc aaggaagagg
     2521 gcgaggagat caagtccaag ctcgaggccg tcggcgcgaa ggtcgaactc aagtaagatt
     2581 ttttatccca tccaccttgc tccctgtctg agcagggggc cgcagtccag aaggaggaca
     2641 actaatggct aagaccaccg aaaagtacga ggtcctgtac gttctcaacc ccaacctcac
     2701 cgaggaagag acccaggcca tcgtggaaaa gttcaagact ctgatcgagc agaacggcac
     2761 cgtcgatgag atggaagagt ggggcaagcg caagctcgcc tacgagatca actacctcac
     2821 tgagggctac tatgtgctgg tgaagttcac ctccgggccc gagcttcccg ccgagctgga
     2881 tcgtatcctc ggcatcaccg acggcgtgat ccgctctctt gtcactctgc gctacgaata
     2941 agcggaggga atgatatgct gaatcatatc gttcttatgg gccgcctgac gcgggatccg
     3001 gagctgcgct ataccggctc caacgtcccc gtcgcgtcct tctccattgc tgttgaccgg
     3061 gatttcggcc gcggtgaaaa cggtgaaaag cagaccgatt tcatcaacgt gtccgcctgg
     3121 cgtcagaccg gggaattcgt ctccaagtac ttcacgaagg gcagcatgat cgtggtttcc
     3181 ggtcggctgc agatccgtga ctacaccgac cgcgacggca accgccgtac cgcggcggaa
     3241 gtcgtggcgg acaacgtcta tttcggcgaa agcaagcgcc gggatgacgg agccccgcgg
     3301 caggagtacg cgccccgtca ggagtacgca ccccgcaccc cctccaacga gggctttaac
     3361 agcgcgaaag cgcaggccgg atcgttccag acgatcgacg atgacgacgg ccagctgccg
     3421 ttttgacact cacgaaagga gtttttgaac catggctttt gataagtccg ctgccccgca
     3481 gaagaaccgc aagcgcagaa aggtctgcca gttctgtgtg gataaggccg agtatatcga
     3541 ctacaaggac accgtcaagc tgcgtaagta cctgtccgag cgcagcaaga tcctgccccg
     3601 ccgcaccacc ggcgcctgcg cctaccatca gcgtctgctc accgaggcga tcaagcgtgc
     3661 ccgtcaggtc gctatcctgc cttacgtgac cgactgattt cggtttttta tcgacaaaaa
     3721 agctctcatc ctccgggatg ggagcttttt ctgttgcaca gcggcagatc ggcggttata
     3781 atgaaagtcg caaaaagcaa agcatgcaac agtatatata tgcggaggtt gacgcgatca
     3841 tgtacgataa cggcaaaatc tatctcggtc tctccggcga ggagcgggtg gagcttcccc
     3901 tctccatgtg caaccggcac ggcctgatcg ccggtgcgac cggtaccggc aagaccgtta
     3961 cgatgaaggt gctggcggaa tccctctccg atgcgggcgt gcccgttttc ctgtgcgacg
     4021 tgaagggcga cgtcgccggt atctgcgccc ccggcgcgga cagcgaggat atgcaaaagc
     4081 gtatcgagcg ctttgccctc accggcaaat ttgcctaccg cggctacccc accacgttct
     4141 gggacattta ccagaccggc ggccatgccg tgcgcgccac cgtgagcgaa atgggccccg
     4201 agctgctctc ccgcattctc ggcctgagcg aggcgcagac gggcgttctg cagatcgttt
     4261 tccgcgttgc ggacgaccgc ggcctgctgc tcgacgatct caaggatctc cgtgcgctgc
     4321 tgaactatgt gaacgaccac aaagaagact atcgcatgaa gtacggcaac atcaccacgc
     4381 agtccgtcgc ggcgatcctc cgcgcgctgc tgccgctgga gaaagagggc ggcgagctgt
     4441 tcttcggcga gccggcgctc gacgtgaacg actggatccg caccgatgcc gagggacgcg
     4501 gcatggtcaa cgtactcgac tgcgtcaagc tcgtgcagaa cccgacgctg tacgcgagct
     4561 tccttctctg gctgctctcg gagctgttcg agaccatgcc ggaggtcggc gatcttgaaa
     4621 agccgaagct cgtgttcttc ttcgatgagg cgcatatgct attccgcgac gccccggcgg
     4681 tgctcgtgca gaagatcgag cagaccgtga agctgatccg ctcgcgcggt atcggcgtgt
     4741 tcttcgtgac gcagagcccg gcggacgtgc cgaacaccgt gctcgcgcag ctttccaacc
     4801 gcgtgcagca cgcgctgcgc gcctatacgc ccaccgagct gaaagcggtg cgcgtggcgg
     4861 cgcaggcgtt ccgcgaaaac ccggcgttca acgccgagga cgcgatcatg gaactcggcg
     4921 tcggcgaggc gctcacgtcg ttcctcggcg aggacggcat ccccgccatg gtgcagcgca
     4981 cgaagatcat ctgcccgcag agcctgatgg gtgtgccgga ggcgatgacg cgcgccaagg
     5041 cgatcctccg cgacggcatg gaaaagtacg acgaagcgga ggacaacgtc tctgcttatg
     5101 aggtgctcgc cgacgaaacg caggccgcgg aggaggcgct cgcgcaggag caggagcggc
     5161 tggaagagga aaagcgcgcc gccgaagagg aaaagcagcg ccggaagcag gcggaggcgg
     5221 acgaaaagcg cgcgcagaag ctcgaagacg aagcgcgccg ccgcgcgcag aagctcgagg
     5281 atgaggagcg ccgccgcgcc cagaagctcg aggacgaggc ccgccgcaag gccgagcgcg
     5341 aaaaggagaa aaaggaagcg gaggaaaagc gcaaagccga acgcatccgc tccaagatcg
     5401 aaacgcagct catctccgcc ggcggccaga tgctcaagcg cggtctgctc ggcatcctga
     5461 aaaagtaaaa cgcagtacgg gaacccctcc ggcttttgcc tgcggcaaaa cgcgccgcct
     5521 ctgtcagggg aggcaggtaa gatcgcggca aaccggcctg gctcccctga taggggagcc
     5581 ggcacggcgc aaagcgccgt gccggaggga ctagtgctcc tgctgcaaat catatatacg
     5641 gacacggaaa gccgctttgg cggctttccg ccgtttttga gaattattct ttacggagaa
     5701 ctgataccca tgataaccat ccgcgaatac gtccgtcccg cctcgcttga agaggcgtgg
     5761 gcactcaacc agaagcgcac gagccgcgtg atcggcggca tgctctggct caaaatgcag
     5821 aatgccgccg tcggcacggc gatcgatcta tccggtctcg gcctcgacaa aatcgaggaa
     5881 acgccggagg gctttgccgt cggcgccatg gtctctctgc gcatgctcga aacgcacgcg
     5941 gggctgaacg agtacacaaa cggcgcgatg tgcgaagcgc tgcgccatat cgtcggcgtg
     6001 cagtttcgga ataccgcaac gctcggcggg agcgtttacg ggcggttcgg cttttccgat
     6061 gtgctcacgg tgctccttgc gctgaacgct tcggttgagc tctataaggg cgggatcgtc
     6121 ccgctggcgc agtttgccga aatgccgttt gaccgggata ttctcgtgcg cgtgcacatc
     6181 ccgaaggaaa atgccgcctt tttctatcag tccgtgcgcc cgacaaaaac ggacttcccc
     6241 gcgctcacct gcgccgcggc gcggcttgcg gacggagcat accgcttcgc tgtcggcgcg
     6301 cggcccggaa gagcggtcgt gcttacgccg cagtgcgcgc tgggtgagct gccggagatc
     6361 gtgagcgcgc aggtcgcgac cggctccaat ctccgcggca gcgcggcata ccggaagcat
     6421 cttatccgcg tgctcgtccg ccgcgccgtg gacgaactgg aaggaggcgc gaagtgaata
     6481 tcacgtttac gctcaataac aaagccgtgt ccgcggatat cgcaccggac acgctgctgc
     6541 tcgaccttgt gcggcgtctc ggctgcaaaa gcgtcaagcg cgggtgcggc accggcaact
     6601 gcggtctctg caccgtgtat ctcgacggca cacccattct ctcctgcgcc acgcttgccg
     6661 cgcgcgtgca gggccggagc gttacgacgc tcgaggggct gcaaagcgaa gccgcagagt
     6721 tcggcgcatt cctcgccgac cagggcgcgg agcagtgcgg cttctgcaac agcggcttta
     6781 ttatgaatac gctcgcgctc ctgcgcgaaa accccgaccc ggacgaggac gagatcaggg
     6841 agtatctcgc gggcaatctc tgccgctgct cgggctacga ggggcagctg cgcggcatac
     6901 agagcttcct cgcgtggaaa aaggcgaagg aggcgagcga agaatgaaca ccgtcaatca
     6961 gggcgtgcgc aagctggacg ccatgcagct cgtgaccggg cagccggtct acaccgagga
     7021 catcgcgccg gaaaactgcc ttgtggtaaa gcttctccgc tcgccgcacg cgaacgccat
     7081 ggtacgcggg atcgacaccg aacgcgccat gcgcgtgccg ggcatggaag ccgtgtttac
     7141 gtggaaggac gtgccgcaga acgcccggcg ctatacgcag gcc
//
LOCUS       DAWAAE010000107         7056 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4677_length_7056_cov_3.051421, whole genome shotgun sequence.
ACCESSION   DAWAAE010000107 DAWAAE010000000
VERSION     DAWAAE010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_7056_cov_3.051421"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4211..4220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1260 a         2225 c         2206 g         1355 t
ORIGIN      
        1 acgaagtggg tctcgtttga gagcttgtca aaaatacttt tttgacaagc tcaaagaccc
       61 atccgaagat gggtcttagt gcgtcgaaaa attagctcag ttgccggttt tatcgagctg
      121 gcgctggcgg gcgaaattct gcatgccgtc ggtcatggag tcgagacggc tgagcatacg
      181 gcccgcgccg taggccgtgc aggagagcgc gtcgtccgcg acgtgcgctt cgatgccggt
      241 gcgctcggtg acgaggcggt caatgccgta caggaggctg ccgccgccgg acatgacgat
      301 gccgttttcc gagatatccg ccaccaactc cggcggcgtg cgctcgagca ccatatggac
      361 ggtctcgagg atggtctccg tcggctcggc gagcgcttcg accatttcgt cggagttgag
      421 cgtaatggtg cgcggaagac cggtcatcag gcagcggccc ttgacctcct catagctgac
      481 ctcggggcgt tccttgacgc agccgatgct ctttttgagc tcctccgccg tgcggatgcc
      541 gatgagcagg ttgtgcttgc gccggacgta tttgacgatc gcctcgtcaa acgccgcgcc
      601 cgccgttttg atggattccg actccaccac gccgttgagc gatacgaccg ccgcttccgt
      661 tgtgccgccg ccgatatcga tgatcatatg gccgtccgcg cggttcacgt ccagacccgc
      721 gccgaccgcc gtggcgaccg ccgaggggac aagatacact tttcgtccgc ccgcctcgat
      781 gacggcgttc acaatggcgc gctcctccac gccggtgatg ctgcccggca cgcacacgag
      841 cacgcgcgag cggaagaggc tgaagctcgt cacgcggcgg atgaactcgc gcagcatttc
      901 gcaggtcatg tcgtaatcgg agataacgcc cgcggcgatg ggatggacgg atacaaggtt
      961 ccccggcgtg cgaccgagca ttttccgcgc atcctcgccg accttaagca cgcggccggt
     1021 cgtacggtcg atcgtaacga ctgtcggctc gcgcaggcga atgccgatac cggcgacata
     1081 gaggatggtc gtatcggtgc cgagatcgat ggcaatatct ttttcgatga tggaggacag
     1141 aataggcata gtcaaattcc cttcaaatca aaagcgatta aattcttctg ttattcttcc
     1201 cggtggcagg caagcgccgc gccgcggacg ctcacagccc cggcggtcag cagcatgcgc
     1261 gcacattcgg aaagcgtgga gccggtggtg atgacatcgt cgataagcag gatcctctta
     1321 ccgcgcaccg cgtccgcact tcggacaaca tagcagccgg agatgttcgc ccggcgctcc
     1381 tccggagtac gcagccggga ctgcggcgaa acgtcgcgcg ctttgcgcag cagcggcaca
     1441 gcctttgttc caaggacctc cgccgccgct tccgcgagaa gctgcgcctg atcgtacccg
     1501 cggcggtgac agcgcttcgc gctcaggggc acccacgaga tgagatcaaa atcgtcagcg
     1561 ccgcggcgga gcagatcctc gccgaggatc cggccgaaaa cctttccgcg cgacggggcg
     1621 ctgcgaaact tgtaggcgag aatgccctgc cgcaccggcg gttcatagta aaagggcgcg
     1681 gtataggcgc ggacaaagtc gcattttcgc tcggtatccg ccgtgcgcgg cagcgctttg
     1741 cggcaggttt cgcacacgcc gtccccgctt ttctccagga tcgcgccgca gaaagcgcag
     1801 cgcggcggga agaaaaagtc gagaacgaag tctttgaagc tcatatcaaa cctctccggc
     1861 cagacgggcg cgcagcccgc tgtaccggcg cgttttgcgg ccgttttcga tcatggcctg
     1921 cacgatcgcg gtttcgccga caacgacgag aagctctctc gcgcgcgtga cggcggtata
     1981 gagaagatcg cggctcaaaa gccgtgccgg ggctcgtccc gctgccagaa caacggcgcg
     2041 gtactcgctg ccctgcgcct tgtgcaccgt gacggcgaag gcatgatcca gctcgccgag
     2101 ctgttcaaag ccataccatg cgagcttttc gtcaaagtcg atccagataa gctcgttttc
     2161 ggtgtcgata cgcaatatcg tcccaagatc gccgttaaag attcccgtgc cggtctcggg
     2221 agcggagccg ggagccattt ctccggctat gatcgccgcg gtatcgccgc ctttacacca
     2281 tactatatca tagttgttcc ggatttgcat cacccgatcg ccctcccgga acacagtttc
     2341 gccaaaaatt ttttcttttt ttccgggcgc gggcggattg agcgcctgct gtaagcgctc
     2401 gttgagcgcg aaggtgccgg attcgccctt gcgcgtcggc gtcagaacct gaatatcgcc
     2461 ggacgggata cccatgccgc gcggcagccg ttcgctgcaa agctcggcca cggtcgcggc
     2521 cagacgctcg cccgagggac gctgcaaaaa gaagaaatcc cctttatttt cgcgcagctc
     2581 cggcgtttcg ccgcggttga tgctgtgcgc gttgcggatg atgcggctct ccccgctctg
     2641 gcggaagatc tccgtcaggc gcaccgccgc aaccgcgtgc gaacggagca catcgagaaa
     2701 aaagctcccc ggcccgacgc tcggcagctg gtcggcgtcg ccaacgagta cgagacggca
     2761 gcccttcggc agcgccttta acagcgcctg aataagcgtt atgtcaacca tcgagcattc
     2821 gtcgagaatg accgcgccgc agcggagcgg atcgctctcg tttttgcgga acgtgagctc
     2881 gtccccctcg gcggcccatg ccgcgccgag cagacggtgg atggtgtagg cctcccggcc
     2941 ggtgagctcg gtcatgcgct tggcggcgcg gccggtaggc gcggcgagaa ggcagtcgag
     3001 ctgcatatcg tcgtacaggc gcagtatggc ctgaatgatc gtcgttttac cggtgccggg
     3061 cccgccggtg acggcgagca gccgccgctc gcaggcgagg cggatggcct ccttctgctg
     3121 cgcggcatag cgcatgccgg tctcacgttc cagctccgcg atacgctcgt ccacgttttt
     3181 ctttgccgga acggtgtcct gtgccatggc gcggagcgcc gcggcggtgc aggtctcggc
     3241 ctcgtacagc cgccggagat acaccgcgtt cacccctgcc acgatctcct gcacgatctc
     3301 gccctcgtcg gcgagaacgt ccagacattc ctccgcggcc tcggcctcca cgccgatgag
     3361 ctgcgccgtt gccgcaacga gcttttcgcg cgggaggaag ctgtggccgt tattgagatt
     3421 atgtaccatt tcaaaaatga gcgccgcggc gatgcgcggg ggactgtcct cctcaaagcc
     3481 gagcgagagc gcgagcgcgt ccgcctcgtc aaactgtccg ccgatgcggt cggacgcgat
     3541 gacgtagggg ttggacttca cgagctcgag cgctccgtcg ccgtagagct tatagagccg
     3601 catggcatat tcgggcttga ggccgttcgc ggcaagaaat tccatcagaa gccgcatacc
     3661 ggtctgacgg cggaacgttt cggaaatgtc ccgcgcgcgg cgcgcgctga tgcccttgat
     3721 ctcggcgagc ttttcggggc tgtcggacaa gatctccagc gtgtgcgagc cgaagcggtt
     3781 cacgagcacc gccgccgtcg ccgggccgat gccgcgcacc gcaccggagg cgaggtattc
     3841 atagatatgc ttctccccca ccggcagcga gcgcatggct gaggacgcct tgaactgctc
     3901 gccgtggctc gggtggcgct cccacgctcc ctcgaccgtc agtccctcgc cgggcgcggc
     3961 gaacggcaga cagcccacga ccgtcaccgg ctcgtcgccg ccgacatcca gacgcagaac
     4021 ggtgtagccg ttctcctcgt tctggaagat cacgcttttt acggttccga tgagctgcag
     4081 ggagccgcgg agttcgtttg cttcgtccat agtgttacat tcccctgtcc cagcgataaa
     4141 tgccgggcga tttctcttgt ttcctcgtcc atgccgtcgt ccagcagcac gatccttcca
     4201 aaaagcacgc nnnnnnnnnn cgccgtcgtt gatgagccac agcaggccat ccaccgtcgc
     4261 ttccagctcc ggcgacgcgc cggagacgcg caaaatgagc tcaaggctct gccccttccc
     4321 gcggcacacg ggcgtgagca tcacgccgcg cagcttccag cacaggaaga gcaccgccgc
     4381 cgcgatcaga aacgccaata tccattttcc cataccgcca tccctccggt atatcctatg
     4441 agggacacgc aggaaaggtt cacttccggt agcgctccaa tgccggtttc agaaattctc
     4501 ccgtgaagct ggcggggcac gcggcgcatt cctccggcgt gccggcaaag acgagctccc
     4561 cgccgccgtc gccgccctcg gggccgagat cgatgatatg gtcagcgcac tggatcacat
     4621 ccagattgtg ctcgatgacg atcacggtgt tgcccgcgtc cacgagccgc tgcaaaacct
     4681 ccacgagctt gtggacgtcc gccatatgca ggccggtcgt cggctcgtcg aggatgtata
     4741 cggtgcggcc ggtgtcccgg cgggagagct ccgtggcaag cttgacgcgc tgcgcctcgc
     4801 cgccggagag cgtcggcgag gactggccga gacgcacata ccctagaccg acatccatga
     4861 gcgtgcgcag cttcggtacg agcttcggct gattttcaaa gaacgtgagc gcttcctcca
     4921 cggtcatatc gagcacttcg gcaatgtttt tgcccttgta gcgcacctcg agcgtttcgc
     4981 ggttgtagcg cttgcccttg cacacatcgc acggaacgaa cacgtccggc aggaagtgca
     5041 tttcgatctt caaaaggccg tcgccggagc aggcctcgca ccgtccgccg cgaacgttga
     5101 aggagaaccg gcccgggccg tagccgcgga gcgcggcatc cttcgtcgag gcgaaaacat
     5161 cgcggatatc attaaagagg ccggtatacg tcgcgggatt gctccgcggc gtgcggccga
     5221 tgggcgtctg gtcgatagcg atgaccttat cgacgtattc aaggccgtcc acgcccgcac
     5281 acttgccggg gcgcgccttc gtgcgcatct tttcggcggc aagcgtttta tacagcacct
     5341 cgttcacgag cgaggacttg cccgagccgg acacaccggt cacgcagata aactcgccga
     5401 gcgggaagcg gacgtcgata tttttaagat tgttctccgt tgctccgcgc acggtgatgg
     5461 cctggccgtt ccctttccgg cgtgtgccgg gcagcgggat acgcttgcgg ccgcttaaat
     5521 aatcgccggt gatgctgcgc ggctcggcgc agatatcctc cacgctgccc tgcgccacga
     5581 cttcgccgcc gtggacgccc gcgccggggc cgatgtccac gatatagtcc gccgcgcgca
     5641 tggtgtcctc gtcgtgctcg acgacgatga gcgtgttgcc catgtcgcgc aggtttttca
     5701 tcgttttgag cagctttgcg ttgtcgcgct ggtgcaagcc gatgctcggc tcatcgagaa
     5761 tgtagagcac gcccatgagc gaggagccga tctgcgtggc cagccggatg cgctgcgctt
     5821 cgccgccgga gagcgtcgcc gcggcgcgcg agagcgtgag atactgcaga ccgacgctct
     5881 gcaaaaaacc gagacggctg cggatctcct tgataatgcg cccggcgatc attgcctgca
     5941 tatcggtcag ttgcggttcg agcgcgtcaa ggaagcgcag acactccgtt acggggagat
     6001 cgcagaactc ggagatattc attccgccga cggtgacggc aaggacctcc ttccgcaggc
     6061 gacgtccgcc gcagtccggg cagggcgttt ccgccatgca ctgctcaatc tccgcgcgca
     6121 tggcagggct ctgcgtttcg cggtaacggc gctcgaggtt ggccgcgacg ccctcgaagg
     6181 gctggtagag cgttcccttg ccgcgctgct gatcgtaatg gagcgtcagc ttctcgccgc
     6241 ctgtgccgta gaggatcact ttgcgcgcct cttccggcag gtcacggatg ggggcatcaa
     6301 gactgaaatg gtattttttc gagagcgctt caaaatacat gcgcgagatc gtgtcgccgc
     6361 ggacattgtt ccagccggac gccacgatgc cgccgccgag aatggagagt gacgggttcg
     6421 gcataatgag ctcgggatcg acgcgcagct gcgtgccgag gccggtgcag ctcgggcagg
     6481 cgccgtaagg gttgttgaac gagaacgcgc gcggcgagag ctcttcgatg gagatgccgc
     6541 agtcctcgca ggcgtagttc tgcggggaga tcgaccgtca caaggccgcc cgtgagcgag
     6601 gacgcggttt cgatcgaatc cgtcagacgg cggcggatat ccggcttcat cacaagacgg
     6661 tccacgatga tctcaatgcg gtgcttttta ttcttgtcca gttcaatggt ttcggaaaga
     6721 tcgtagagat tgccgtccac gcgcacgcgg acataaccgc tcttgcgcgc gtcctcaaag
     6781 accttggcgt gctcgccctt cttgccgcgg atgaccgggg caaggatctg gagcttcgtc
     6841 ccctccggca gcgccgtcac ctggtcaatg atctggtcaa tggtctgctg gcggatctcc
     6901 ttgccgcact tcgggcagtg cggcgtgccg atacgcgccc agagcagacg gagatagtca
     6961 tagatctccg tcacggtgcc gacggtcgag cgcgggttgc ggctcgtcgt tttctggtcg
     7021 atggaaatgg cgggcgaaag gccgtcgata tagtcc
//
LOCUS       DAWAAE010000108         6970 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4734_length_6970_cov_2.761678, whole genome shotgun sequence.
ACCESSION   DAWAAE010000108 DAWAAE010000000
VERSION     DAWAAE010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4734_length_6970_cov_2.761678"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1369..1378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4485..4494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1456 a         2039 c         2116 g         1339 t
ORIGIN      
        1 cggcgtcaac ggcaacatca tgtttgtaga cggcggcacc gacgctctgc tcaacagcga
       61 gaaagtttac taaggaggcg aaacccatga ctttgattcg acagtatgtg gacgctctgc
      121 tcgaccagga cgcgctggct ttgagcaagc tcttttctcc gacggccatt tttgttgacc
      181 actgcccccg cgatgcggga aagccgctcg tcagcaccta cggccctgag gccatcgata
      241 tgttctacca caaccgcttc ctgttccgcc acttccgtat caccgatgcc cgtatcgtca
      301 gcgacacgca ggcctactac tatgccgtct atgacggcta tcatgtccgc gctctggcca
      361 ccatcacgga attcagctcc gacggcctga tcgagcggct ggtcgtaaga ccggcataag
      421 atcccggcgg gccattctgt aaatcgaata agacaagttc gcggcggcgt ctcttttgag
      481 acgccgccgt ttatccgcaa aaaacgcgtt tttgggcttg tatatatgta tgtatattat
      541 aatgggttta tacgttttcc cgaagaaaaa gatataggga gggtaaatct atggtagcgg
      601 ttattattat tgcagtgatc gtagtgcccg tagtggcgta ttacatcgga aaggaatttg
      661 agtatatcgc gaacgaaaag gggtatacaa gcaagaagta tttctggtgg acgttcctgt
      721 ttggcgcggt cggtatctca atggtcatcg cgctgcctga tcggcgcggc ggaacgggcg
      781 cggcgtccag cggtctgccg atgagcgacg acgaaatccc ggagatatga ggtgcggaca
      841 tgagtgagag atatacgcgc ctcttcgcac tggaagaaaa tctgtattcc gaaggttccc
      901 caatcgtcat ttctgcgggt gcattgctca aagataaccg aaagtgagcc tgagtgagga
      961 gtgactcctt ccgtctcggc tgacgccgag ccactccctc aaagagggaa gcagttaaaa
     1021 agaaaacgca gcagctccat atcacggagc tgctgcgttt gcgtatctta gtctttcttg
     1081 aaggaatcct tgagcgcgac ggcgcggtta aatacgagat ggccgggggc gctgtccttg
     1141 ttgtccggcg caaagtagcc gagacggagg aactggaagc ggtcggcagc ggcgcagtcc
     1201 tcgagcgact tttccacctt gcagccggtg agaacctcga gcgagttggg gttcaggcag
     1261 tcgatgaagt ccttgccggg tgcgtccggt tcgggatcgg agaagagcat atcgtagagc
     1321 cggacctcgg cgtcggcgca gttgttcgcg tccacccagt ggatcgtgnn nnnnnnnncc
     1381 ttgcgcccgt cgggcgtgtt gccgccgcga gtctcggggt cgtattcgca gagaatctcc
     1441 gtcacgttgc cgtccgcgtc cttcacgcag ccggtgcagc ggacgatgta ggcgccctta
     1501 agacgcactt cgttgcccgg atagaggcgg ttatacttct tgaccggctc ttccatgaaa
     1561 tcgtcctgct cgacccagag cgtgcgggag aacgagaccg ggcgcgtgcc ggcgttttcg
     1621 gcgacggggt tgttttcgac atcgaaaagc tcagtctgcc cctcgggata gttcgtgatc
     1681 gtgagcttca cgggacggag caccgccata acgcgcttgg cgttcgcgtt taagtcgtcg
     1741 cgcaggcagc tttcgagcag cgagtagtcc accgtggacg tgaccttgga aacgccgatg
     1801 cggtcggtaa aatcgcggat ggagtgcggc gtatagccgc ggcggcgcag accgcagagc
     1861 gtcggcatcc ggggatcgtc ccagccggac acgcggccct cttccacaag gcggcgcagc
     1921 ttgcgcttgc tcatcacggt atagttgatg ccgagacggg caaactcgat ctggcgcggc
     1981 ttggacggca cgtcgcagtt ctcgatgacc cagttgtaga gcgggcggtg atcctcgtat
     2041 tcgagcgagc agagcgagtg cgtgatgccc tcaagcgcgt cctgaatggg gtgggcgaga
     2101 tcgtacatcg ggaagatgca ccacttcgtg ccctgccggt ggtgctcgat gtagcggatg
     2161 cggtagagca ccgggtcgcg catgttgaag ttgccgcttg cgagatcgat cttcgcgcgc
     2221 agcgtatact tgccttcggg gaactccccg gcgcgcatcc gcgcaaagag atcaagcgat
     2281 tcttcgatcg ggcggtcgcg gtaagggctg acggcggggt gcgtcgtgtc gccgcggtaa
     2341 gcggaaaact cctcgggcgt cagctcgcag acgtaggcga gacccttttt gatgaggccg
     2401 acggcgagct cgtaggtctt ttcaaagtag tccgagccgt agaaaaagcg gtcgccccag
     2461 tcaaagccga gccagcggat atcctcctga atggcatcga cgaactcggt gtcctccttg
     2521 gcggggttgg tgtcgtccat gcgcagattg cagagaccgc cgaacttctc cgccgtgccg
     2581 aagtcgatgc acagtgcctt gcagtggccg atgtgcagat agccgttcgg ttcgggcggg
     2641 aaacgggtgt gcaccgtttt tccggcaaac cgtccgccgg gagcaatgtc ctcggcgaca
     2701 aaatcatgaa taaaattgct ggattctctg atctcttcca tattgactct ctccatttta
     2761 tgaaagctga atgaatgaaa attattatac acgtcaacgc ttgaaattac aacagaatta
     2821 ttggataata gaagcgctat aaatctttcc gccctttggc ggaacaaacg acaggagtat
     2881 gccatatgaa atatgatgcc gtgatcctcg gcggcggccc ggcggctgtc tccgcggcgc
     2941 tcacgctgcg cgcgcgaggg aagaccgccg cgatcctctc cggcgggatc gacgatatcc
     3001 cgctctcccg cgcaagcagg attaccaatt accccggctg tccggatgtc tccggccgcg
     3061 cgctgctcga agcgatggaa catcaggcgc tcgacgcggg cacggaaatc cttcacggcc
     3121 gtgccacgtc gatcgcgccc ctcggcgacg gcttcggcgt gatctacggc gcggacttct
     3181 gcgaggcgga aacactcatc ctctgcaccg gcgttgccgc cggaaaagtt ttccctggcg
     3241 aacgggagtt cctcggccgc ggtgtgagct actgcgtcac ctgcgacgga atgctctatc
     3301 gcggaaaaaa ggtctgcgtc gtgggcttta cgtccgatac gaaagaggaa gcggagcttc
     3361 tgcgcacgat gggctgcgga gttgaaatgt tcacgagccg cacggcgaag tacgccgtgc
     3421 tcgggcagga gcgcgtcacg gcgctttccg tgaacgggga ggagcatccg tgcgaggcgg
     3481 tattcatcct tcgcccggcc gcggcgcccg atactctgct cgcgggtctt gccatggacg
     3541 gcgtgcatgt cgccgtggat aaggcgacga tgaaaacctc cgttgccggc gttttcgccg
     3601 ccggggactg caccggcaag ccttaccaga tcgccaaggc cgtcggcgac ggtaacatcg
     3661 ccgcgctcag cgccgcgcag tatatcgaag aaaggagccg ggagaaaaag gcatgaacga
     3721 gagcgagatc aaatatctgg agcttctttc caagagcttt ccgagcatcc ccgctacggc
     3781 ggcggagatc gtgcggctgc aggccattct ggagctgccg aaaacgaccg agcacttcat
     3841 gagcgacatc cacggcgagt atacatactt ctcccatatc ctcaaaaacg cctccggcgc
     3901 gatccacgac aagatcaacg acagcttcgg cgatcttctc ggcgaggagg agaaaaattc
     3961 gctcgcccgg ctgctctatt accccgagcg ggagcttgcc gccgcagcgc actccgattc
     4021 ctggtaccgc gtgacgatcc accgtcttgt ggccgtctgc cggcaggcga cggacaaata
     4081 tacccgcgtc aaggtgcgag cccgtctgcc gaaggaatat gcctatatta tcgaggagct
     4141 cattgactcc gacgagcacg atcccaacaa gcgcaggtat tttgaaagca tcatcaacag
     4201 cgtgatcgag accggcattg ccgaccggtt catcgtggcg ctcgcaaatc tcatctcccg
     4261 cctgacggta aaccagctgc acatcgtcgg cgacgttttt gaccgcggtc ccgccgcgca
     4321 gatggtcatg gacgatctca tggacatgca cggcgtggac ttcgtctggg gcaaccacga
     4381 cattctctgg atgggcgcgg ccatgggcaa ccccgcgtgc attgcgaacg ccctgcgaaa
     4441 ctcgctcaag tacggcaact ttgatatgct cgaggacggc tacgnnnnnn nnnntggagc
     4501 agtacggcga cgatccctgc acgaacttcc tcccgacgca cgtgaccgac tgcgtggcgg
     4561 aaaacagcga cgttaccgcc aagctcctca aggccatcac ggtcatccag ttcaagctgg
     4621 agggccagct catcctgcgc cacccggaat ataaaatgga cggacggctc ctcctcggag
     4681 agctcgtccg ctccgaaggg acggtcacgc tcggcggcaa gatataccgc acgaacgaca
     4741 tttcgctccc cacggtcgat cccgcggatc cctatcgcct ctccgagcgc gagcagcagc
     4801 tcgtcgatca gctcgttttg tccttcctgc gcagcgagaa gctgcagacc catatccggt
     4861 atctgctgga aaagggcgcg atgtaccgcg tgtgcaacgg caaccttctc taccacggct
     4921 gcatccccat ggagccggac ggcagcttta cgcgcgtgac gatgggtgac aaaacatatt
     4981 tcggaaagtc gctcatggat gcctgcgacc ggctgtgccg cacggcgatg tacgaccgcc
     5041 gtatggagaa caccgatctt ctctggtatc tctggtgcgg gccgtattcc ccgctcaacg
     5101 gcaaggacaa gatcgcaacg ttcgagcgca cgttcctcga cgatccggag gcccgaacgg
     5161 agcagaaaaa tccgtactac gatctctgga acagtgagga gacggcgcgc acgatccttg
     5221 ccgagttcgg cgtgaatttt gaacgcggcc acatcatcaa cggccacgtc cccgtgcgca
     5281 aaacgaaggg cgacacgccc atcaaggcca acggcaagtt catcaacatc gacggcggtc
     5341 tctcgaaggc gtaccagccc gtaacgggca tctgcggcta tacgctcgtg tattcctcgc
     5401 acgagctgta tcttgccgag caccagccct tcgacccgga caccgccgcg cgcgagagcg
     5461 cagacgtcct ctcccgcaca attatgatcg agcagtaccc cgaccggctt cgcgtacgcg
     5521 ataccgacgg cggcgcggat gtggagcggc gcatcgccga tctcaaaaag ctgctcgcgg
     5581 cctaccgcag cggccttgtg cgcacgccga gccgcatgga gtaaggcttg acaaataaaa
     5641 caaataacgg tatattgagt tggtaaaaag cgcagaaggg gaggagtagg ctcccggcgg
     5701 aacgcagaga gagggcggtt ggtgcaagcc cttgggaagc cgcagccgaa ggtcgccccg
     5761 gagcagccgt tttgaacaaa gtagaaacgg acgggttctc ccgttacaga gaatacgagt
     5821 gccgctttca gtaaagcgga aatcgggtgg taccgcgtaa gtttttgcgc cccggaggtt
     5881 atctccgggg cgtttttgtt tggaggaata agaatgaaag aactgccgaa gatttatgat
     5941 cccaagcagg tggaaagcaa gatctacagc tactggacgg acggcggctg gttccacgct
     6001 gagcgtgacc cggacaagaa gccgttcacc atcgtgatcc cgcccccgaa cgtcaccggg
     6061 cagctccatc tgggccatgc gttcgacgag acaattcagg acgtgctcat ccgctggaag
     6121 cgcatgagcg ggtactgcgc gctgtggctg ccgggcgttg accacgccgg catcgccacg
     6181 cagaccaagg tggaggccga gctccgcaag gaggggctca cgcgcttcga tctcggccgc
     6241 gaaaagttcc tggaccgtgt gtgggactgg aagcagcact acggcgaccg catcgtggag
     6301 cagctcaaaa cgctcggctc ttcgtgcgac tgggagcgcc agcgcttcac gatggacgag
     6361 cagtgcgcca aggccgtgcg cgaggcgttc tgcagcctgt acgaaaaggg atatatctac
     6421 cgcggcaagc gtatcatcaa ctggtgttcg cactgcaaga ccgccctttc cgacgttgag
     6481 gtcgaatacg tggagaaaaa cggcttcttc tggcatatcc gctatccgct gacggacggt
     6541 tccggcagcg tggaggtcgc cacgacccgc cccgagacga tgctcggcga cgccgccgtc
     6601 gcggtgaacc ccgaggaccc gcgctataag gacatgatcg gcaaaacgct cacgctcccg
     6661 ctcgtcgggc gcgagatccc catcgtcggc gatgagcacg ccgatatgga gttcggcacc
     6721 ggctgcgtga agatcacgcc ctgccacgac cccaacgact ttgaggtcgg cctgcgccac
     6781 gatctgccgc agtacctgat cctcgacggc gacggcaaga tcaccggcgg ctacaagtgg
     6841 gacggcatgg accgctacga ggcccgcaag gccatcgtgc aggagctcga ggagcagggc
     6901 tatctcgtgt ccatcgagcc gtgcgtccac aatgtcggca cgtgctcccg ctgccataac
     6961 gatgtggagc
//
LOCUS       DAWAAE010000109         6946 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4759_length_6946_cov_3.097809, whole genome shotgun sequence.
ACCESSION   DAWAAE010000109 DAWAAE010000000
VERSION     DAWAAE010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4759_length_6946_cov_3.097809"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5847..5856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1473 a         1978 c         2124 g         1361 t
ORIGIN      
        1 ttcggaacgt atgacgagat ctgggcaatt gcccggcggg ccgaccggct cgaggaactc
       61 aaaaatcaca cggccttccc ggtacgtacc atctcactcg atctttccga cccggcgtcg
      121 tatgagacgt atgccgccct cctgcgcgag gagcagccgg aggtcggcct tctcatcaac
      181 gccagcggct tcggcaaatt ccgcgccgtg atggatacgc cgctcgaggt caatctcaat
      241 atggtcgatc tcaactgcaa ggcggtcatg gcgctctgcc agctcacggt gccgtatatg
      301 ccgtccggca gccagatcat caatattgcg tctgttgcgg cgtttcagcc catcccgtat
      361 atcaacgttt acggcgcgac aaaggctctt gttctgcact acagccgtgc gctcaaccac
      421 gagctgaaaa agcagggggt gcatgtcatg gcggtatgcc cgttctggac aaagaccgaa
      481 ttctttgacc gcgccgtggc gtcggacgaa aagccggtcg tgaagaaata cattgccatg
      541 tacaagcccg aggagatcgt cgcgcaagcc tggaaggacg cgaagaaggg caaagacatg
      601 agcaaatgcg gcttcaaggc gcgcatgcag gcgctcggcg tgaagatcct gccgcacagc
      661 atggtaatgg acacctggct ccgccagcag gaactggatt aaagaaaatc aaaaaccggg
      721 gctgcacggc attatcggct gtgcggcccc ggttttttgt ttcaggtatt ttcgcttctg
      781 cggcggcgat gcccgcgccc gcggcggctc tgcgtcgcct tgtcgcgata gaatttctcc
      841 atctcgtcgt cggcgcggta gacgatgcgg ttcaccgacc agctgtcgtt ctccgttttg
      901 cggaacttga gccggacgag gaatttgcgg tgctcctcgc atgtaccgat tgtcatatac
      961 cgtctatcgc cctgattgac ccaacgcgtc agctccagcg gctcgccgca gtcgggacag
     1021 acggcgctcg tgacctcttc atcgagaaac gcctcagagc gggaattata tccggtgaag
     1081 gcgcggtgct cgagcgcatc cggttcatcg gtgtggtgct tatcctccgg aagtcgagtg
     1141 gcgagaaggg aagtggcgtt gtcgtactgc tccagtccct cggcaaagtc gagcttggag
     1201 cagacgagcg ccgtgttgta ggcgtcgccg agcgcgtcgt gcgccacgcg ggtctgctca
     1261 atggcaaagt gctccatcgc cgtggcgagg gatttctggt ttttgtcgcc gccggtctgg
     1321 atgttgtaaa tgagctggag attgatccag tctgcgatcc agtcccagtc gagatcgtgg
     1381 atgatgatgt tctgctccat gatccgccgg tcatcgtagc cccaggtgac gaacaccggg
     1441 tcctcgccgc accattcgcg gaaatgctca aataccgtgg ggaacgtgtc gccgtggctg
     1501 aggcgctcct tgtcaatgcc ggtcagcttt ttgactttgt aatgcatgct gcggaagtat
     1561 atgggccgca cgtcggactg aaattcctcc gcggggcaga aatcatcgtc gagctttacc
     1621 gcgccgatct ggatgatctc gccgctcaga tggatgggga gacggttgaa tacggacgcg
     1681 ttggagctca tagcctggtt ccattccagg tcaaggacaa tataggacat tcggattacc
     1741 gacctttctt ataaccaaat cattatacca atgcatcagg gaaataacaa tccctttttt
     1801 ccggaaaaat cacagctgcg gcggacagtt ttcgcagaac acactttttg cagaccaaaa
     1861 agcaaatatt gtgtttccat tctccagcag gtgatgtata ataaaaagag atgcaaacat
     1921 gggaggcatg actcttgtac atagctgtta tctgcggcgg attgagcgcc gagcgcgatg
     1981 tttccatcag ctccggcacg ggcgcggcac gcgcgctgcg tgcgcgcgga cacaaggtcg
     2041 cgttggtcga tctttttctc ggaaacccct cggcggtgaa tgatccccgc agcgtcttta
     2101 cgtccgacgc cggagactcc gtttactccg tgcgggagac cgagcccgat cttgacaaga
     2161 tccgcgccca gcgcgccggc aaagggctga tcggccccgg cgtgatcgag atctgccgcg
     2221 ccgcggatat cgcgtttctc gcgttgcacg gcgcggaggg cgaaaacgga aaactgcagg
     2281 cgtgtctgga tctttacggt gtgccgtata ccggcagcgg ctatctcggc agcgcgctcg
     2341 ccatggataa ggaattgagc aagatccttt tccgcagcat cggcatccct acgcctccgg
     2401 gcgtcacgct ccgccgcggc gacgcggcgc cggagagcat cggctttccg tgcgtcgtga
     2461 agccctgctc cggcggctcc tccgtcggca cgagcattgt caccgagccg gaaaagctcc
     2521 ccggggcgct cgaagaggcg ttttcctgcg atgaggcggt gcttgtagag aagtatatcc
     2581 gcgcccgcga gctgacggtc ggcgtgatgg acggccgcgc catggcggtc attgagatca
     2641 ttcccaagac cgggttctat gattataaga acaagtacca ggcgggcctt accgaggaga
     2701 tctgccccgc gccgatcagc gcggaggaca ccgagcgcgt gcagcgcctt gccgagcgcg
     2761 tatacgaagc tctccatctc gatgcctatg gccgtgtcga tttcctgatg gataaagcgg
     2821 acggtgagct ctactgtctg gaagccaata cgctccccgg tatgacgccc acaagcctca
     2881 tcccgcagat ggcggcggcc gagggcatgg attacggcga gctgtgcgag aaaattatcg
     2941 aagtatcttt gaagaagtat aagaaatgaa cggaatgacg atcggcgaaa tgctccgcgc
     3001 ctgccgcggt gaatggcagg gagccgaaga gaccctcacg aaagcgccct cgtccatcgt
     3061 gaccgacagc cggcaggcgg gggagggaag cctctttctc gcgctgcgcg gcgagaaaac
     3121 ggacggacat aagtacattc ccgacgtgct gcggaagggc gcgcttgcgg tgctgtgcga
     3181 ggagcgcggc gcggcgggtg agccgcgcat cgttgtgccg gatacgctcg cagccatgca
     3241 gcagatcgcc gcggcaagcc ggaagcgctg ggacattccg tttatcggcg tcaccggcag
     3301 cgtcggcaag acgacggcaa aggaaatgct cgccgccgcg ctctccggaa aatacgaagt
     3361 attcaaaacg ccgggaagca tgaacgggca gatcggcatt cccgtttcac tcatcggcct
     3421 gcacggagag tatgacgttg ccgtgatcga aatgggcgtt tccctcttcg gggagatgac
     3481 gcgccttacg aacatggtgc atcccgatat ggcggtgttt acgaacatcg gcgacgcgca
     3541 tctggaggcg ctcggcgacc gtcccggtat cttgcgggcc aagtctgaga tattgcaggg
     3601 catggggccc gatgccgtca tcttcgccaa cggcgacgac gcgctccttt ccgcagcgga
     3661 cttcggacgg aagaccgtcc tcttcggtct tggtgaaaac tgcgctgtcc gcgctgcgga
     3721 tgttcagcac tgcggcgaga gtctttcctg ctgcattctt gcaggggagc gcagctttcc
     3781 cgtgactgtc ccggcctacg gcacatatat gatctactcc gtgctcgctg cggcggcggt
     3841 atcgcttgag ctcggccttt cggacgcgga gatcgcgcgc gggctcgccc ggtatgaaac
     3901 ggtgggccgc cgctctcgtg tcgtaaaaac cgggtactgc acgcttgtgg acgattgcta
     3961 taacgccaac ccgacgtccg accgcgcggc gatcgactcg cttgcctcgc tttccggacg
     4021 gcacgtatgc attctcggcg atatgctgga aatgggcgga aacgctacgg agcttcaccg
     4081 gtcggtcggt gaatacgccg cggcgcacgg cgttgatctt gtgatcacgc agggggagct
     4141 ggcaaagtac attgccgaag gcgcggggac ggccggcgcg cattaccccg accgcgcatc
     4201 gcttctcgcc gcgctcccgg agctgctgca tgagggcgac gtggtgctcg tcaaagcctc
     4261 ccacggcgca catttcgagg aaatatcgga agccgttaag cggctttctt gaaaggctca
     4321 aaaatcctgt gctccggcac aggatttttt gtaacgaaac gggaaaaacg cgcacttata
     4381 tacgacgaca cagaaaggag agcggtacgg atgcagagca tggaagagat ctaccggcag
     4441 cacgcgcaga cggtgtaccg ctatctcctt gcccagacgc gcagcgccga tcttgcggag
     4501 gagctgacgc aggagacgtt ctatcaggct gtgcgcaccg ccgggcagta ccgcggcgaa
     4561 ggaaagctca ccacatggct ctgcggcatt gcgaagaacg tcctgcgcac ctatcagcgc
     4621 cgccatccgc cggaagagga actggatgaa acggcggctg ctccggacac gccggatgaa
     4681 gcgtttctcg tgcgtgaatc gcagctggag ctgctgcgga aaatgcagac gctcgggagt
     4741 gacgcgcgcg aggtgatgta ccttcgcatc ttcggcgcgt tgtcgttccg ggatatcggc
     4801 gaaatactct ctcacacgga gaactgggcg cgcgtcacgt actaccgcgg cagggaagca
     4861 ctgcgaaagg agctggaaaa agacgatgaa aaatgatctc agctgcgagg tcgtccgtga
     4921 ccttctgccc tcgtaccttg acggcgtggc gagcggcgag acgaaggccg ctgtggagcg
     4981 gcatatggaa gaatgtccgg actgccgcga aacgctgcgc cggatgaagg aaccggagga
     5041 gaccgcgctg acagaagaga aggagatcga ttatctcaaa aaggtgcgcc gacgcagcag
     5101 ccgcaaagtg gctgtaattc tggcgatcgt cgtgcttctc tcgatggcag cgatgttccg
     5161 gctgttttat attggctttc ctgtggactc gcgcgcgatc gccgcggtcg tcaccgtaag
     5221 ggacggtacg gtgcgtctca ccggcacgct taccgacagc ggccagagcg tgtgccgggc
     5281 gaacatcacg cagaacgagg acggcggcgt ggaggtgcag atgtatgctg cgctcccgct
     5341 cttcgcgcat agcggagact ttacctggga ataccccgcg gcgaaggatg tcaccagcgt
     5401 atcggtcaac ggccttgttc tctgggaaaa cggcgaagcg atcgactcgc tcacggcgag
     5461 gctctacgag gcgaagaatc cctatgtcgg tgatatgagc gcgaacgcta atattgcctc
     5521 gctcctccgc gtcggaaacc gcgtcggcag ctttacgaat gaattgcaga cggacgcaga
     5581 gccctacggc tggacgctta ttctggacga tcccatcacg gcggagaacg agaagaacac
     5641 aaaagaagag atgcgccgct gctcctgtgt gctgctcgcg gagatcgaaa atctcggcac
     5701 cgtttcatgg aagtatgaga ccgaagcagg ggagaagacc ttcaccgtca ccgccacgga
     5761 cgcatcaaag ctggcgggac aggatatcaa gacctgcgcc gcctctgcct cggttatcgg
     5821 caaaaccttg tatatagtcg gtcgcannnn nnnnnntaac acaaaagcgc ccgtgcttcg
     5881 gcacgggcgc ttttgcgata ttggatcaat ctttttcaaa tacaacaacg ccgcattcgt
     5941 agggatggtc gtagtatacg gcttcctttc cgcgctgcct gtggagcagc attttgagta
     6001 taatggcaaa atccccgccg ccgccgaaga tcatggctaa tgccagaatg aagacccaga
     6061 aaatcccggt cagagccgcg gcggccgtca ggccgatacc aagcacgagc gtcggcatgg
     6121 ccgcgccgag aatatactgc ccgcgcttca tcgactgcgc acaggtgcag taaggcgtaa
     6181 gcgcattcca gatcacgccg aagcgaatgg ctctccagtg actttcggcc aaaagacccc
     6241 atgtaagtcc atggatgcct tcgtgaactg cggtaagaag cagcattaca agaaggaata
     6301 agagaaaatc gaaaggcgac atcgattcgg ccatcgagag gaatgaaacg cgggaaagaa
     6361 caacggctcc ggagagtgcg gcaaacggta gcatgacgat aagcgccaaa aggttcgcct
     6421 ttactacgcc gatggtgaga tcggtctttc ggtatccctg ccgttccatt tcctcacaga
     6481 cgagcgcaaa ctgttccttg cgtttctgct cggcgggggt gagctttctc tcatttttcg
     6541 ccattgtatc ttgctccgat ccacagattt tcatcattgt accacggcag acagacgttt
     6601 gcaagaaagt ataaaactcc ggcatgaccc tgcggatcat gccggagctt atcgctatat
     6661 gcttagatca ggtggaaggc aaggatcagg ccggagaaca cgatggaaac cgtggagcac
     6721 agcttgatca gaatgttcag ggacgggccg gaagtatcct tgaacggatc gccgacggtg
     6781 tcgccgacaa cggcagcctt gtgctgctcg ctgcccttgc cgccgtggtg gccgccctcg
     6841 atatacttct tcgcgttgtc ccaggcgccg ccggcgttgg acatgaacac ggccatcgca
     6901 aagccggtga cggacacgcc gccgagcaga ccgacaacgc ccgtgg
//
LOCUS       DAWAAE010000110         6919 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4780_length_6919_cov_3.058858, whole genome shotgun sequence.
ACCESSION   DAWAAE010000110 DAWAAE010000000
VERSION     DAWAAE010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_6919_cov_3.058858"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1317 a         2233 c         2044 g         1325 t
ORIGIN      
        1 agactttttc ggttctttcg ccctgtctct cggcgacagc ttagctaata taacacactc
       61 gatttcggtt gtcaacacct tttttcgatt tttttcaaaa aacttttttc gttttttgat
      121 agcctcgaaa tcggcgtctg gcggggttcg ccgggcgaat gagccgcccc cgatctctca
      181 cgggagcggc tcattcgaaa tccctctttt ttcttaaaaa gagcgatctc agacggcccg
      241 cgtgaccttg ccgctgcgga ggcagcgggt gcacgcataa acgtgcttcg gggtaccgtc
      301 gacgatggcc ttgacgcgct tcacattggg cttccaagtg cggttggcgc ggcggtggga
      361 atggctgacc ttgatgccga aagcgacatc cttctcgcag tattcacact ttgccatact
      421 tgctgcacct cctcatcgag aaatgtgctg cccttttggg gtgccggcga ccggtctccc
      481 gcggacagct tacttataat agcagaccgc gccgccgaat gcaagatgaa atttcatttt
      541 cttttaaaaa acttctcttt actcggctgt tgccaaatcg ggcggaattg attacaatat
      601 aatattatat cacttatcac cttgatacgg aggaacgctc atggctcagt cctattccgt
      661 cagtctggat accatcgtcc gcgagcacaa tctggccatc gtcaaccgcg cggtggacta
      721 tgaccgcgtg cagattacca catgggaggt ctcgcgtccc ggactgccgc ttgccgggtt
      781 ttacgattac tttgacgcca cacgcgtaca gatcatcggc aagctcgaga tcacttattt
      841 aatggatatg cgtcccgagg cgcgcagccg cagcattgag aggttcatct cctccgggat
      901 ccgcttctgc atcgtcgcgc acggcatgga tatcccgccc gagatgctct cctcggcgca
      961 gcgccatcag gtgacgctgc tcaccaccga gcactccacg agcgatttca tggcaaagct
     1021 catcgtgagt ctcaaccgct ggctcgcccc gcgcaccacg atgcacggcg tgctgatgga
     1081 ggtccacggc gagggcgtga tgatcacggg cgagagcggc gtcggcaaga gcgaaaacgc
     1141 catggcgctt ctgagccgcg gccaccgtct tgtggcggac gacgccattg aggtcaagcg
     1201 caccgcgccg gacacgctcg tcggctcttc gccggagctg atccggtatt tcatggagat
     1261 ccgcggcatc ggccttatcg acgcgcggca catgttcggc atcggcgcgg tgaagcccga
     1321 ccaggtcatc gaccttgtcg tgcattttga gccgtgggac gagaacaagg tctacgaccg
     1381 gctcggcaac gagacgcatt tcacggagat cctcggcgtg aagatcccgt actttgtcgt
     1441 gccggtgcgg ccgggccgaa atcttgcagc gatcctcgag cttgcggcaa tgaacaaccg
     1501 cgagcgcaaa atgggctaca acgccgcgca ggtgctcgtg gagcgccacg accggatggt
     1561 ggacgaggga tgagaaacgc cgaactccgt tccttcctcc gggaagcgcg ctcccgctgc
     1621 ccggagctca cgatcctcga aaacgagccg atgagccgcc actgctcgtt ccgcatcggc
     1681 ggcccatgcg acgcgatgct gctgccgtcc tccgtttcgg aggtcgagac agtctgtgct
     1741 ctgcttgccg aagccggaga aaagccgctt ctcatcggca acggcacaaa tcttctcgtc
     1801 accgacgcgc cgctgcaccg catcgtgctg cggctcggcg aggagttttc ccgcattgag
     1861 ccggtcggcg gcgatacggc tctccgcgcc gagagcggcg tgacgctctc gcgtctcgcc
     1921 tcgtttgccg cggcgcgctc gctcgccggg ctggaatttg ctcacggcat ccccggcacg
     1981 ctcggcggcg cggtatccat gaacgcaggc gcctacggcg gggagatgaa ggatgtcgtc
     2041 acctccgtaa cgtatctcga cgaagacctt tccgtccgcg agacggacga cgccggtttt
     2101 tcttaccggc acagccgctt ttccgatacc ggctgcatcg ttctcggcgc agaagtttcg
     2161 ctgcacgagg acgatcccga cgccgtccgt gagcggatgc gctcgctcgc cgagcggcgc
     2221 agaagctcgc agccgctcga catgccgtcc gccggcagca cgttcaagcg tcccgcaggc
     2281 ggatacgccg ccgcgctcat tgacgaggcc ggtctcaagg gctgtgccgt cggcggcgcg
     2341 caggtgagtg aaaagcacgc gggcttcgtc gtcaaccgcg gcggagcgag ctttgacgat
     2401 gtgctccggc tcattgaaca cattcaaaac gaggtatacc gcgtctccgg cattgagctg
     2461 gagccggagg tcaagatcat ccgcgaataa aaagatcgcg gggaaagata gattatacta
     2521 tggaatttct gatcgtcacc ggtctttcgg gcgcgggcaa aacgcaggcc gcgcactttc
     2581 tggaggatct ggggtattac tgcgtagaca acatgccctc ggcgcttttg cagccgttcg
     2641 cggaattctg catcgcctcg caggggcgct ttaccaaagt tgcccttgtc acggacgtgc
     2701 gcgcgcacga gagctttgaa cagctcttcc gcgcgctcga ttcgctgcgc gcgctcgact
     2761 gcacgttccg catcctgttt ctcgaagcgc cggagagcac gctcgtccgc cgctacaagg
     2821 aaacgcgccg ccgccacccg ctcgcggagc cgggcaaaac ggtgcaggcc gcgctgcggg
     2881 aggagatcgc gctcatgcag ccggtgcgcg accgtgccga ctacattata aatacgcaga
     2941 atctcacgct cgcgcagctg cacaaaaagc tctgccgtct gttcaccgag accggcgagg
     3001 caaagctgcc gatcaacgtg atcgccttcg gctacaaata cggcatcccc atcgatgccg
     3061 atctcgtgct ggacgttcgt tttctgccga acccttatta tatagaggag ctgaaggatc
     3121 tctccgggct cgaccagccg gtgtacgact ttgtcatgca gcagccggac acagcagaat
     3181 acatgcgcct gctcacggcg tttatcgatt ttcagctccc gcgcttccgg gaagaaggca
     3241 agccggcgct caccgtggcc gtcggctgca ccggcgggcg gcaccggagc atttccgtgg
     3301 cgcgcgcgct cacggatcat ctgctcgagc gcgggcagaa cgcgcggctc gtctgccgcg
     3361 atcttgcgag ggtaaacgaa caatgaaaaa acgcatttcc cgccgtccgg atatgaaaat
     3421 cgccgccatc ggcggcggca ccggactttc caccatgctg cgcggcctga agcgcatttc
     3481 accgtccatc accgccatcg tcaccgtgat ggacaacggc ggcggcagcg gcgtgctccg
     3541 gcaggagctc ggtatgctcc cgcccggcga catccgcaac tgtctgcagg cgcttgcgaa
     3601 caccgagccg acgatggagg ccctgctcgc ctatcggttt caggacggcg cgctgcgcgg
     3661 ccagagcttc ggcaatctct ttctcgccgc gctcaacggc atgtgcgaca cgttcgacga
     3721 ggccgtcgcg cgcatgagcg aggtgctcgc catcaccggg cgcgtgctgc cggtcacgaa
     3781 cgagaacgtg cagctcctcg ccgattttga tgacggcgtg accgtcacgg gcgaatcgca
     3841 gatcgccgaa tacaaaaaag atctcacgcg gcgcatcgtg cgggcatatc tctcgccgga
     3901 gcatccggcg gcgctgcccg cctgtcttga cgccatccgg gaggccgatc tcatcgtgct
     3961 cggccccggc agtctttata cgagcgtcat tccgaacctt ctcgtctccg gcatccgcga
     4021 ggcgatccac gagtcggacg ccgtaaaggt ctatgttatg aatcttatga cgcaaagcgg
     4081 cgagaccgcg gggtataccg ccgtcgacca cgtccgcgcg ctcttcgacc acggcggcga
     4141 gggactgttc gactataccc ttgtaaacga tcttcccatc ccgcccgaag cgcaggagga
     4201 atatctccgc gaaggggccg ttcccatcac ggcggacgag gaagcgctcg ccgcgctcgg
     4261 cgtcactgtc cggtatgcgc ccgtggcgct ctggcaaaag gcgctcgttc gccacgatcc
     4321 cgccgcgctc gccgcggcgc tgcgcgatct ttaccgtgaa gcctccccca cccgcattta
     4381 cggataaaca cacaaagggg tttttcccat gtccttttcg tccgaagcca aaaacgagct
     4441 gtgccatgtt ccgctcaacc gcacctgctg cgcgagggcc gaggcctacg gcgtgctttt
     4501 atactgccac acgttcgacc ggcgggagat ccgcatcatc acggcgaacg acgcctttgc
     4561 ccagcgtctg ccgaagctct tccggcgcgc gttctcgctc tcgtttgacc atgtgccgcc
     4621 ggagggcgcg gcgggcaagc gcacattcct catcacagcg ccggaaaaga tcctctcgat
     4681 cctggaggtt ttcgggcagg agagcgccgt ttcccaccac gtcaactacg gcatgctcga
     4741 aaccgactgc tgccggcaga gcttcttccg cggggcgttt ctcgccggcg gcagcgtgac
     4801 tgatccggac aagcgctacc atctcgaact gctcacgagc cacccgagcg tgagccgcga
     4861 ggcgttcaat ctcatgctgg aaatgggctt tgcgcccaag gacgccgtgc gcagcggcgg
     4921 atacatcacg tatttcaagc agtccgaagc cattgaagac gtgctcacaa cgatcggcgc
     4981 gccgctcgcc gcgatgggca tcatgcaggc aaaaatgcag aaggacatgc gcaacgccgt
     5041 caaccgccgc gtcaactgcg acagcgcgaa cgccgacaag atcgtcctcg ccgcgcagga
     5101 gcagctcgat gccatccgtg agctggaccg gaaatacggg ctcgacacgc tcccggatat
     5161 tttgcaggaa acggccatgc tgcgcatcgc caaccccgag tcgagccttg ccgatcttgc
     5221 gcggctcgcc agccccccgg tgtccaaaag ctgcatgagc caccggttaa agaagctatt
     5281 agagtacaaa ggagactgat atgggctttg tccatctgca cgtgcatacg gaatactccc
     5341 tgctggacgg cgcctgccgc gtcaaggagc tcgcgcgccg cgccaaggag ctgggacaga
     5401 cggcgctcgc cgtcacggac cacggcgtga tgtacggcgc ggtctctttt taccgcgcgt
     5461 gtcttgccga gggcatccgc ccgatcatcg gctgcgaggt atacgttgcg ccgcgctccc
     5521 gctttgaccg tgagcacggc atcgataacg agtattccca tctcatcctt ctgtgcaaaa
     5581 acgacgtcgg ctaccggaat ctctgctatc ttgtctcctg cggctttacg gacgggtttt
     5641 acatcaagcc gcgcatcgac tgggcgctgc tgcatgagca cgccgagggg ctgatctgtc
     5701 tttccggctg tctcgcggga tatctctccc ggcacatcgt cgcggacgat tacgagggcg
     5761 caaagaaaaa ggcgcttgag ctgcgcgggc tgttcggcga ggactttttc ctggaaattc
     5821 aggatcacgg catcccggac gagcgcaagg ccgcgcttgg gctgcgccgt ctctcgcgtg
     5881 aaacgggcat cccgctcgtc gtgacgaacg acgcgcatta tattaataag gaagacgccc
     5941 gaaatcagga cgtccttctc tgcatccaga ccggcaagac cgtggacgat cccgaccgga
     6001 tgaagttttc ctcccccgag ctttatctca aaagcgagga ggagatgcgc gcgctcttcc
     6061 ccgaagagcc gcaggccgcg gacgtcaccg aggagatcgc cgcgcgctgc cattacgatt
     6121 ttgagttcgg ccactaccac ctgccggaat ataagctccc ggagggggaa acggacagct
     6181 ttgcctatct cacgcgcctc tgccgcgaag ggtacgtccg gcgctacggc gagaccggca
     6241 cgagcgagat ggaagcgcag ctccggtatg agcttgacat gatccaccgc atggggtttg
     6301 tggactactt cctcatcgtc tgggactttg ttcactacgc gaaaagccac ggcatccccg
     6361 tcggcccggg gcgcggcagc gccgcgggca gcatggtctc ctatacgctc ggcatcacgg
     6421 acgtggaccc gatccggtat tcgctctttt tcgagcggtt cttaaacccc gagcgcgtga
     6481 gcatgcctga tatcgatatg gacttctgcg tgcgccgccg cggcgaggtc atcgactacg
     6541 ttacgcgcaa atacggcgcg gaccacgtcg cgcagatcgt caccttcggc acgatggccg
     6601 cgcgcatggc catccgcgac gtcggccgcg cgctgaatct cagctacgcg gaaacggacg
     6661 ccgtcgccaa gcaggtgccg agcggcccgg gcgcgctcaa catgacgctc gacgaagcgc
     6721 tcaagctctc caagcctctg aaggacatgt acgagagcga cgagaaggtc aaaaatctca
     6781 tcgacaccgc ccgcgcactc gagggcatgc cgcgccacgc ctcgacccac gccgccggcg
     6841 ttgtcatcac gcaccggccg gtgtacgaat atgtcccgct cgccacgaac gacggcaacc
     6901 ccgtcacgca gtatacgat
//
LOCUS       DAWAAE010000111         6893 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4808_length_6893_cov_3.449254, whole genome shotgun sequence.
ACCESSION   DAWAAE010000111 DAWAAE010000000
VERSION     DAWAAE010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4808_length_6893_cov_3.449254"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5973..5982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1326 a         2068 c         2129 g         1360 t
ORIGIN      
        1 gagtatgccg tctacaactt tccctcctgg gacgaatgcg ccgcgcgcgg ctggtcgatc
       61 accgacgcgg taaagcgcga aaatgcgtat ttctccgtgc ggtatgcagg agaatttctc
      121 ggcttttttc atatcatgga ccgcgccggt cacgtggagc tcggtgtcgg gatgaagccg
      181 gagctctgcg gccagggccg cgggcggatg ctcatggcgc tcgcgctcgc aaagatcctc
      241 gaaacgcgcg gctgcacaac ggtgaagctt gccgtgcgca cgttcaatac ccgcgccgtc
      301 cgctgctacg agcgtgccgg gttcacgatc atcgacacgc gttatgaagc tctcggcacc
      361 gcgcccggag aaatgtacat catgcagacc acagtttgat ctctctcaaa cgagttcccc
      421 ttcgttaggc tctctcatgc gagggggcct ttttctgttt atgcataaaa ttaacaaaaa
      481 gtaacgttat gtaacccata atatgaatat tgggaggccg gacaagccgt attcactgtg
      541 gaaaactctg tgggaaatgt tcaaaactct tttatttcct attatttccc ggcgcgggac
      601 aaaaatgaat attcataatc tattcataat aggatgagag gagaagggag gcgcgtccat
      661 gcgcaggttc atttgcgcgg ctctggccgc tctttacctc acgataccgg ctctcgccgt
      721 ggagacgctg ccggaggaga gcttttcgct ttcctgcacg gcggcggtgc tgatggaacg
      781 gcagaccggc acggtactgt atgagaagga agcccaccgg catctctccc cggcgagcgt
      841 gacgaaggtg atgacgctgc tgctcgtggc ggaggcggtc gagagcggcg cggtctcgcc
      901 ggacgatccc gttacggcga gccgccgggc ggcctcgatg ggcggcagcc agatctggct
      961 cgaagagggc gaggtgatga ccgtcgcgga gatgaccaag tgcgtggcgg tcgtctcggc
     1021 gaacgactgc gccgtggcgc tcgcggaggc gctctgcggc tcggaggagg cgttcgtcgc
     1081 gcgcatgaac cgccgcgcgg aggagttggg tctttccgat acgcatttta ccaactgcac
     1141 cggactcttc gaggacgcgg agcactacac ctgcgcctac gacattgcgg tcatatcgcg
     1201 cgagctattg aagcacgaat tcgtccggca gtatacgacg ctctggcagg acacgatacg
     1261 ttcgggtgaa ttcggcctta cgaacacgaa taagctcgtg cggcactaca acggctgcac
     1321 ggggctcaaa acgggcttca cgtccacggc gatgtactgt ctggccgcct ccgccgagcg
     1381 cggcggtgtg gagtacattg cggtcatcct ccacggcgag acgagccagc tgcgctttga
     1441 cgcggcgcgg acgcttttgg actacgcctt tgccaattat gcgctcgtca tgccggagac
     1501 cggcgcgccg gacgtgccgg tgtcactcgg caaggcggcg agcgtacgcg ccgtgtgcga
     1561 gagctccggt acggtgctca tcgaaaaggc gcggcagaag gagctttccg ccgtttacga
     1621 actgccggag aaggtggacg cgcccgttgc cgccgggcag cttctcggta cgctccggta
     1681 cgttgccgga aacgaaacga tagcaagcgt tccgatcagc gcggcggaga gcgtggagcg
     1741 cgtcggcgcc ggggagctgt gggtgcggct cgccgcggcg ctgtgcggcc ggacaaatac
     1801 gcaatagttc atcttgcaag tgagcgtcgg agagtgtaca atgtcgacaa aggaagaaat
     1861 taaccgcagg gaggtatacc aatgattcat gttgatctca ccggcgccgc gccgttcttc
     1921 ggcgcacaca ccccggacta tgccgccgcg gaagcggcgc accgcacgct cagcgaaaag
     1981 acgggcgcgg gttcggattt tctcggctgg acggagctgc cgcgccgcat ccgcgaaacg
     2041 gagcttgtgc gcattctcgc cgcgggcaac gcgatccgtg aaaagggcga cgcgctcgtc
     2101 gttgtcggca tcggcggatc gtatctcggc gcacgcgcgg cctatgagct gatcggccgg
     2161 cgcgagggcg gcgcggagct gctctttgcc ggaaacgggc tttccgccgc gacgctctgc
     2221 gacacgatcg aaaagctcgg agagcgtgat ttctgcgtga acgtcgtttc gaaatccggt
     2281 acgacgctcg agccggcgct cggcttccgc gtgttccgcg atctgctcat caagaaatac
     2341 ggcgaggaag aggccaaccg ccgcattttc gccacgaccg acgcgcgccg cggcgctctc
     2401 aagagcgagg cggacgccgc cgggtgggag agcttcgtcg ttcccgacga cgtcggcggg
     2461 cgctacagcg tgctctccgc cgtcggcctg ctgccgctct gcgccgcggg catcgacatt
     2521 gaaaagatcc tcaccgtcgc cgaggagacg ttcgcctctc tcgacgagcg cagcgaagcg
     2581 aacccctgtt ggcagtacgc cgccgcgcgc caggcgctct acaagagcgg caaggccgtg
     2641 gagatcctcg cgtgctacga gcctcgcttc cgcatgatgg cggagtggtg gaagcagctt
     2701 tacggcgaat ccgagggcaa gaacggtctc ggcattttcc cggcgagcgt ggaatataac
     2761 gccgatctgc actccatggg tcagtacatt caggaaggcc cgcgccatct gatggagacg
     2821 gtcgtgagct ttgcttctct ccccggcgga tacaccgttc ccgccgacgc gcacgacggc
     2881 gacggactca attatctcgc cggacacgat ttcaacgaga tcgccgccat tgcccgcgcg
     2941 gcggtaaaga ccgcgcatat cgagggcggc gtccccaatc tcgccattga ggtcacggac
     3001 atggcagaga ccggctttgc cgagctcgta tgcttctttg agcttgcctg cggcatttcc
     3061 ggctatatgg agggcgtgaa cccgttcgat cagccgggcg tggaggccta taaaaagctc
     3121 atgttccgcg gtcttgccgc gctcaaatga agaacattcg atgaacattc gaaaagctcc
     3181 ttgcaacggg cggcggaaga tgatagaata cagaaaaaga agcgttcgca tctttctttc
     3241 aaatttacaa atgaggtgat ttcctatggt taaactcatc atgggtctgt ccggctccgg
     3301 caagaccaag accctgatct ctctcgtcag cgaagccatt gaaaaggaac agggcaatgt
     3361 tgtctgcatc gaaaagagcc ggcatctgac ctatgatatt ccctatcagg cgcggctgat
     3421 ccgcaccgac gattacggcg tccgcggcaa cacgatgctg ctcggcttcc tgtgcggcct
     3481 gcacgccggc aattacgaca tcacccattt cttcatcgac gatctcaaca agatcctcga
     3541 cgaccgctcc aacgaggcgc tggagaagtt cctcgacacg ctctccgcgt ttggcgagcg
     3601 cgagaacatc aacttcacca tcactatcac cgccgatcct tccggcgcgg gcgacggtct
     3661 gaagaaatat ttctgatatg cggctcgaca agtggctgaa ggtctcgcgg ctcatcaagc
     3721 gccgcaccgt ggcacaggag gcgtgcgacg gcgggcatgt gtccgtcggc gggcgcgtcg
     3781 ccaagccctc caccgatgtg aaggtcggcg acgtgatcga gatccgcttc ggcgcgaaaa
     3841 cgctgcgggc ggaggtcctc tccgtgcagg aaacggtcaa aaaggacgat gccgccgcaa
     3901 tgtaccgcga actgtgataa aaaaacgcat tctctgtttc ggcagagaat gcgttttgct
     3961 ttttggggtt tcttatttct catccccggc gtgcggctcc gctacgcgga gcatgcggaa
     4021 gccctcgccg tggacctcgt tggattcgag aatgatggtg aatgacgccg ggtcgagcag
     4081 ctttacctcg cgggtaatgg tgaacatctc cttgccggag caggcgcaca gcaccacatc
     4141 gcggtgatcc ttccggaagc cgccgcgcgc gtcgatgatc gttgtgccgc ggccgcagag
     4201 ctcgtcgatc cgctccgtga cggcttcgcc gtggtcggtg acgatgagcg cgagctttcc
     4261 ggcgttcgcg ccatacatga tcttgtcggc aatgacggcg aaaatgaggt cgatcatcat
     4321 gccgtagacg atgccgtcaa aatcgcggaa aatgatgccg ccgagcacca caacgatgcc
     4381 gtccgtgagc atgatgaacc ggccgagcgg caggtgcggg tgcttggcct tgagcgccat
     4441 tgtgatgaag tccgcgccgc cggtggagga gttgcgcata tagatgagcg cgtagcccag
     4501 cccgccgagc acgcccgcgc agatggcggc gacgagccgc tcaccggtat actgcggcag
     4561 catcggggct atgtaatcca cgaaaagcga ggagaggatc atgcaccgca cgctgcagag
     4621 gaaaaaccgc cgcccgagca cccgatagca gaggatcgcc acagggatgt tgagcacaat
     4681 ggtcatcacg ccgatgggta gatcgaacag gcggtataag ataagcgcga gaccggaaaa
     4741 gccggtcatc gggaactttg cgggcagcgc gaagttataa atggcgacgc cggtcagtat
     4801 gcttcccgcg atctccacga gcgtatcgaa taaccatgtt ttcccgaaca gctttcccac
     4861 gatgaccatc tccctttctt ttccttttat caaaaaagag cggacgcgaa ttatgagacc
     4921 gattcgcatc cgctcttttt cattttatca tgcgaaggat ttgcttgtca attccgccaa
     4981 aagcacggcg agatattttt acaattcgac gagctcatac tcgcggttgc cgacgccgag
     5041 agcggcggca cactcgatcg tgtggatgcc gtggagtgac tccacgcgct cgatgaaatg
     5101 cttttggccg gggtcgtccg tcgcggcata gatgagatcg atgcatgcct gatcgaccgc
     5161 gaccgggtcg agcgaggcga gaatgccgat gtccttcatg caggggtctt ccgccacggc
     5221 gcagcagtcg cagtccacgg agaggttttt catcacgttc acgaacgcca tgctgccgcg
     5281 gaaataatcc acaacggcgc ttgccgcgtc cgccatgccc gcaaggaaca cgtcctgcgc
     5341 cggaaggctg tccccgagcc ggttctggtc ggtcgtcgtg ccgccggagt ggatccacac
     5401 cttgcccgca gtggaggcgc agccgatgga gagctgcttg agcgctccgc cgtagccgcc
     5461 catcgggtgg cccttgaagt gggagaggac gagcatggag tcgtagtttt cgatgtcctt
     5521 gccgacgatg tcctttttca gcacgctgcc gttcggaatg ggaagctcca gatcggggcc
     5581 ttcggcatcg agaatgtcca cgtcgaacac ctccgaccag ccgtgcttgc gcaggaggcg
     5641 gcggtgctgc tcggtgagat tgcgcgcacc ctcatacgcc gtgttgcact cgacgacggt
     5701 gccgttcacc gcgtctacaa cgctcttcca gaattcgggg tgcagaaagt tctggttgcc
     5761 ctcctcgccg gagtgcacct tgaccgcgat cttgcccgga agctccttgc cgagagcgcg
     5821 ataaagctcc agcacctttt ccggcgtgat ctcgcgggag aaatatacga tcggcttcat
     5881 tcgtgtgtcc tccttgtcaa cgcttgtttt tccgcttcgc gatggactcc ttgaccgact
     5941 gcacaagatc gccgatcacc ggcagcggtt ccnnnnnnnn nngaacgctc acaatgccct
     6001 tgatcgcgcc gatggtgcag atgatgcctc cgacggtgcc gacccagccc cacaggttgc
     6061 cgagcagact cgtaagaata ccgaagagga tgagcccgag cccctgctcc gtgtggtatt
     6121 tcatgaaggg gtcatccttc tgcgtgatgg cggggatgag ccagaagggg ccgagatacg
     6181 agaatacgga gagcttctgc cgctcgaccg actccgcagc ctgacggcgc atcgtctcgt
     6241 ccgcgggtga gtacgtccgc tgctgtccgg cgttttgggc gttcggctgc gccgagccgg
     6301 tgcgctgcga ggtgtactgc gcctgcgcgg ctccggccgt gcgctgggag gtgtactgtc
     6361 cctgcgccgg gccgccggtg tgcgtctgct gccacgggtc ctgctgccag cgcttctgct
     6421 gctcggcata gcggcgttcg gcttcttctc ttgccttttg ctgcgcctct tcctgcgctc
     6481 tgcgcgcggc ttcttcccgc tcacggcgcg cctgttcggc ggcgcggcgg cgctcttcct
     6541 cggcggcgcg ctgcgcggct tccgccgcct ttttcgcttc ggcctccggg tcgtagccgc
     6601 aggcagggca ctcggtttcc ccgatcggga tatacgcacc gcatttatcg cagaaactca
     6661 acgggaaccc ccctttaata acataatttt ccttatacag tatagcgcgc ttcccctccg
     6721 attgcaaggg cgccccagcg gatgaacgaa aaacgcaggg cgtggcacac cggccccgcc
     6781 ctccacagcc cgtcgaaaaa gcctcgcaga gtttgcgccc gcaggcgcaa atagagtgtt
     6841 aaccattttc tccggcgaca tgtgcgtcgg agaaaatact tttcggctcg caa
//
LOCUS       DAWAAE010000112         6660 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4987_length_6660_cov_3.227858, whole genome shotgun sequence.
ACCESSION   DAWAAE010000112 DAWAAE010000000
VERSION     DAWAAE010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_6660_cov_3.227858"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2774..2783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1355 a         1960 c         1992 g         1343 t
ORIGIN      
        1 accttgtcca cctgcagcgc catcgtcccg gcaaggtcgg cgacggactt tttgtgcgtc
       61 cattcgtcga gcagctcgcc gatgccgagc aggaagatga ccgaacccgc cgtttcaaag
      121 tctccgcgca ggatcgatac ggcaatggct gtcgcgtcga gcaccggcac ttcgatcttc
      181 ccgtgcagta gacagcggat gccgtcgcgc aggaaacgga gtgagcggat cacggtacgg
      241 atcgcgcgca atggcgcggg caggatgagc ttcaatgtat accggcggac gatgcggaaa
      301 atgagctttc cctcgtactc gcggttgagc acgcggacgc tgtgctccgg cgcgagcgcc
      361 gccgacttct cataggaaaa cgcggcgagc gccgcgacga tgggcgcgcg ctgaccggta
      421 taaagaatcg tagcgttgcc ggtgcggtcg tggaccgtca catcggcaac gccgggagta
      481 tttcgcagat acgcctccag aatgtccgct tggagcagcg acatgcgcgg ctgctccaga
      541 tggacacgga tgcgtcccgc cgtatcgtgc aggatcgtgc atttcatggc gaattactcc
      601 gcggcgtcct caatcgtctc gggcgcgtcc tccgcggcat acttctcgtt gagcgccttc
      661 gcgtcggcga gaatgtcgtc cgcgccctcg cggatattcg tcgcggttgt catgacggct
      721 tccttggcgc gcagcgccgc cgcggtcgtg aaagcgtaga ccttcttcgc gtcgcggctg
      781 gtgaggatct tgatgcccgc cgtgccgaac agcacgccgc ccgcgaaaag accaacttta
      841 ccccaattca tgaaagtatc ctccttatct ttttgggatt tcgcccgcat tttatcttta
      901 tgttccggca ttgtcaatag attttctgtt aatatttgca tttttcagca aaatattagc
      961 gcaggcttcc tttagttagt cataattaac tcattgactt cggaatggcg gactgctatc
     1021 atgcatcgcg gaggcgatac tatgaaaacg ttactcaagg aattcaagga atttgccgta
     1081 aaggggaacg tggtcgatat ggcggtcggc gtcattgtcg gcggcgcgtt cgggaagatc
     1141 gtcacatcgc tcgtcaacga cgtcatcatg ccgctcgtcg gcgtgctgac cggcgggatc
     1201 gacttcagca atctttttct cccgctcgac gggaatacct acgccacgct cgccgaagcg
     1261 gaggccgcgg gcgcggcggt gctcaagtac ggctcgttcc tctcgcaggt gctcgatttt
     1321 ctgatcgttt cgttcgtgat ctttattttt gtaaagaaga tcatgacgct tggccggaag
     1381 aaggaagctc ccgtggagga agcgccgaaa acgaaggtct gcccctactg caagagtgag
     1441 atcgccatcg acgccacgcg ctgcccgcac tgcacatccg agcttccgta agaagttcac
     1501 cacgattaac tacagttctg gaggtgaccc atgcagacat ttgagaccga acactacact
     1561 tttcactatg gcgcaaacac cgcggcagag cgcgatatcg agaagatcgc cgcgctgcag
     1621 gagagctgct tctcccacat ctgcgcggtg ctgggtctta cgccggattt caggatcgag
     1681 tacttcctct gcgattcgcc ggaggaggta ggacgcatct gcggcgacga cgagccctgc
     1741 aacggctttg ccgacctgcc gagccgcatc tacgccgtgt ataacgagac cgtccaatgc
     1801 gtcggcttcc acgaggatgc gcacctcatc agctttgtcc acaaccgtcc ggtctgtccc
     1861 gccgtgacag aggggctggc catgtacttt gaccgcaaat ggtggggtat cgaaaatctg
     1921 gactggacgg gctattatct caagaccggg cggtatctgc cgatggacaa gctgctggac
     1981 cgggaggctt tcttcgcgca cgactgcacc ttcacctacc ccatcgcggg cgctttcacc
     2041 gactggctgc tcacggtcta cggcagagag cggtatgggg aaatgtactc ccagccgaac
     2101 cccgccgccg tcatggagca ggtatacggg aaatccccgg cggagctgaa tgccgagttt
     2161 gaagcatacg tccgcctgtt ccgcaccgac gatgcgctgg aagcgcggat ggaagagctg
     2221 ctgcggcaga agggcgttga agcctgacca agcaaaattc tttggcttac aaaaatggcc
     2281 ggatgcgttt gcatccggcc attttattgc tctttattac tttttcgccg ccctgccgaa
     2341 ccggcgtttg acagcgccgg aaagcgtgta gacgcccgcc gcggcggccg caccgccggc
     2401 ggcccagaac agcccgcttc ccgcgccgga cgccttggcc gcaaggtccg gcgccggcgt
     2461 tggaaccggt gtcggagtgg gggaaggcgt tggcgcggct gtcggcgtcg gggaagccgt
     2521 cggagacgga aacggcgtac tgcccgcctg tccgctcccc gcgaaggcat gctcctgacg
     2581 ctcctcgccg tccagactcg gcggacagca caccttacag gcgcggtagc cgcgcgcgac
     2641 ggcgtccgca agcgttattt cgatcttgct ttttttgaga tgtcgacagg agccggtatg
     2701 atagcactcg ccggtgcggg tgatgtaaac gaccgtattg ccgcccgccg ccagcgccgg
     2761 agaaaccagg atannnnnnn nnnggataaa gactgcgagg aagaagccga gaagcttttt
     2821 catagttctt tcaccgccgg atccaatata gcaaagccaa cggaataaag caagcagatc
     2881 cccgccgctg aaccggatac agaaaatcgc agcctgacgg cgcacttttt tcatttaaat
     2941 tgacaattga ctggttcgtg aagtgcggct ataatgacgc caacaagaaa ataccagcaa
     3001 aacctttgag aaagaagagt acccttgcaa aaccgggcct tcagagagct gccgatggtg
     3061 tgagtgcagc ggaacggcgt aaggggaatg gactttcgag ggcggtctga ccgcattcgt
     3121 atggatgcaa gtaggaaccg acgggtgccc acccgttatc caccgggatg cgcatgatgg
     3181 tgcgcatgag cgagcggacg cgaaagcgtc aatttgggtg gtatcgcagg agtacagctc
     3241 ttgtcccata gtgggataag gggctgtttt tttatactcc aaattcagcc gttggcagcg
     3301 gctcctccat ccgccacccg aaaaaacatg gcaaaatgcc aaaaatggag gaaatcacca
     3361 tgaaaaacct gaacctgaaa aaccttgccg tcctcgtcat ggtcttcgcc ctgctgctga
     3421 tgacaacggc ttgcggcgcc ggacaaaccg tatcggacgc ctcgtccgat acacagcctg
     3481 cacagactgc ggacggtgcc tccggaccgg cggcggagcc gcagtccttc cgcatcggca
     3541 tctgcaacta tgtagacgat gcctccctga accagatcgt ggaaaacatt caggcgcggc
     3601 tggaggagat cagtgcggag aagggcgtgc agtttgtgat cgactatgat aactgcaacg
     3661 cggacgccac cgtgatggag cagatcatcg caaacttcca gagcagcggc gccgatcttc
     3721 tcgtcggcgt tgccacgcct gtcgccatgc gcatgcagac ggccacggag gacagccaaa
     3781 cgcccgttgt tttcgccgct gtttccgacc cgatcggcgc gggtcttgtg gagtcgctgg
     3841 aagcgccggg cagcaacgtg accgggacat cggactatct ggatactgcc gccgtcatga
     3901 aactgatctt cgcggctgac ccggatgcat cgaaaatcgg ccttttgtat gacgcgggac
     3961 aggattcctc gacggctgcc attgccagcg ccaaggccta tctggacgag aacgggatcg
     4021 aatacgttga acacaccggc agcacggcgg acgaagtcat gctggctgcg caggccatgg
     4081 ttgccgacgg cgttgacgcc gtatttaccc cgaccgacaa ctccatcatg aaagccgagc
     4141 tcgccattta cgagacgttc atcgatgcgg gcatcccgca gtataccggc gccgactcct
     4201 tcgcgctcaa cggcgcgttc ctcggttatg gtgtggacta cgcaaacctc ggccgcgaga
     4261 cggcggacat gatcgcggac attcttttga acggcgcaga tccttcctcc acgccggtac
     4321 ggacctttga taacggcacc gccaccatca acacggaaac ctgcgccggg ctgggtcttg
     4381 actttgacgc gctctcccag gtgtttgcgc cgtactgcac caagatcgcc gcgctgacga
     4441 cggcggagag cttctcgtga tccagatctg aaaccggatc cggcaaaacg aacagatcgc
     4501 ccggagccgg atgtacgacc ggctccgggc aaattccaaa aaagaagaaa gaggaaaacc
     4561 accatggatc tatcgagtgt tttcggtctg atacagacgg cgctggagct ggggctgatc
     4621 tgctccgtga ccgtattggc gctgtatctg agctattcga tgctgaacgt ctgtgatctt
     4681 tccacggacg gctgcttcac gctgggagcg gtgaccggcg cagtcaccgc gatggccggg
     4741 catccgatac tggcgatttt cgcggcaatg gccgcggggc tgctgtccgg gctgatcgtt
     4801 tccgttttgc agacgcgcat gggtatcaac agcctgttgg ccggcatcat cgtgaatacg
     4861 gcgctgtatt ccatcaacat tgccattatg ggcggcgcgt cgctgctgag tctcaacaaa
     4921 acgcagacgg tgttctctct gatgaaatcc gcgctggccg gcactccgct ggccgggcag
     4981 tataagctcc tgaccgctct cgctgcggcg gcgctgatcg tcctgtttct gacgctgttc
     5041 ctccgcaccc gaatggggct tgcgattcgc gctaccggca acaatccgga catggtgcgc
     5101 tcgtcgtcca tcaacccggc gataaccaca acggtcgggc tgtgcattgc gggcagcttc
     5161 acggctttgt ccggctgcct gctcgcgcag tatcaaaagg cggcggatat caacatcggg
     5221 cagggcatgg tgaccattgc gctggcctcg ctcctgatcg gcagcaccgt cctcggccgc
     5281 ggcgggatct ttcggcgcgc ggtgggcgcg gttttgggcg cgatagtttt ccggctggtc
     5341 tataccgttg cgctgcggct ggatatgccg gcattcatgc tgaagctcgt ttcggctgtc
     5401 atcgtcgtgc tcgccatttc cgttccctac cttaaaacgc agctgccggt catccggcgc
     5461 cggatgcaga gaaaggcgga ctgagacgca tgctggagct gaagagtatt tccaaaactt
     5521 tcaatcccgg aacggtcaat gagaaatgcg ctctgcacga tctgtcgctc agcgtgccgg
     5581 acggagattt tatcaccatc atcggcgcga acggcgccgg aaaatccacg ctgttcggag
     5641 cgatcggcgg aaactttctg acggacagcg gagagatcct gctcgacggc caaaacgtga
     5701 cgttctggcc ggagcaccgg cgctccaaag tcatcggccg tctgtttcag gatccgatgc
     5761 gcggcagcgc gccgggcatg acgattgaag aaaatctggc gcttgccgcc gggcgcggcg
     5821 gctggctggg gcagatccgc cgccgtgacc gcgaatattt ccgcgaacag ctgcgcgagc
     5881 tggatatggg gctggaagac cgcctggggc agccggtggg gcttctgtcc ggcggtcagc
     5941 gtcaggcgct gacgctcctg atggcaacga tagcaaagcc caagctcctt ctgctcgacg
     6001 agcacacggc agcgctcgat ccgggtgcgg cagagaaaat tctggccatg actacgcgca
     6061 tcgcggccga gcagaagctg acatgcctga tggtaacgca taatatgcgc gccgcgctcg
     6121 ctctcggcaa tcggacgctg atgatgaata acggccaaat cgttctggat gtcagcgggg
     6181 cggagcgcgc tcatatggag gtaaacgatc tgctcgagcg cttcaaatcc ggcacgggcg
     6241 ccgcgcttga taacgaccgt atccttcttt cctgatgacg acattctctt tgccgcgaaa
     6301 aactcatagc aacaaaaaag cccgcatctt gcgatgcggg tttatttctt ggtggggcgg
     6361 gtggattcgc ccgcacggcg gcgcgggccg cctcgcggct ctggagcgcc cccggcgctc
     6421 cattcactcc cgctcggatc gaatcccccg ccgggacaat actagaaaaa gcccgcatct
     6481 tgcgatgcgg gcttttggtg ggggcgggtg gattcgaacc accgtaggca ttgccagcag
     6541 atttacagtc tgtccccttt ggccactcgg gaacaccccc atatgcactt gacccaccga
     6601 aaaaaagtgg agctggtaga cggattcgaa cccccgacct gctgattaca aatcagctgc
//
LOCUS       DAWAAE010000113         6650 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_4996_length_6650_cov_3.586808, whole genome shotgun sequence.
ACCESSION   DAWAAE010000113 DAWAAE010000000
VERSION     DAWAAE010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4996_length_6650_cov_3.586808"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1216 a         2063 c         2242 g         1129 t
ORIGIN      
        1 gtaaatgtcc ttgaagtggt agtaaaacgt catgcggttg atgccgcagt cctcggcaat
       61 gtcgttgatg gtgatcttcg tgagcggctt tttgagcagc agatttttga gcgactgctc
      121 gagcgcccgc tttgttacct gtgacatggc gttctcctta tccgatattt tgaattttcc
      181 cgtccgcgag cgcgagaagc gagcgctcct gctcgatatc accctgatac gggtaggccg
      241 cagcgatctt ttcaaaccgg ccgcgcagca gggcggcgcg ctctttgtcg ccgtcgtgca
      301 gcagggcgag ggcgtactcc gtgcgcagca cgctcgggaa atccttcatc tggcgcatga
      361 agcggcgctg cggctttgtg agcatcgcat cgaccgtgcc gcggcggttt tcgccgagga
      421 gctccagata catctcgtcg cacagcaaaa gcgcgcggta gagcccgacg aggttcgccc
      481 cgccggagag caggcgcgac atcgcctcgt gcgcctcggc gaagcggtgc tcgtccatga
      541 ggcggctgca gcagaaaacg ccggtcgcgg cgacgagggc gttctgcatt gcctcgtccg
      601 acggcatgac aaaccattcg tccggcatat cgcgcaggcg ctcgccctcc gtctggcggc
      661 tgtttacggc catttgcaga taaaaggcgc gcatggcctc cggatcgcgg cgcagggaga
      721 aggcgttttt gccgtcgttg tcgatcggcc cgagccggag cgggatgccg ttcgtgagtg
      781 cgagataaag ccccgcgagc gcaaatagaa agagcagaga cgcgcccatg ctgccgccgc
      841 cggagaggaa gtacccgccg agcgcgagaa gcgcggcgag cagattcata agcggccccg
      901 cgagattata aagcatcacc ggaattttcc cgtccacggg ctccggcggc gtcatcaggc
      961 actgcccgcc cgtcccggca agcttcaggc ggcggaagcg aagcgcgccg ttttcgcgct
     1021 gcaattggaa attcccaatg cggaacgagc agaatttata ccctgtcagc agtccgccga
     1081 tcagatggcc ggtctcgtgc aggatgatct gcaaggcgaa ggagacgcag agcccggcaa
     1141 gcccgaagag gaacacggcg aaaaagctct cgttttcggg aagcgcatag ccgacgcccg
     1201 cgccgagccc cacgcagaga acgagcgaga ggatcatccc cggctgaatg gctgatggct
     1261 tcttattctt tgcctttgtg tccatactat cacccgacgg cattataaca gattttttgc
     1321 cggatgcaaa tgcatgacag ccccgcctcc tgcgcggaaa acatttgaaa aaaggacagg
     1381 aatgctgtat actgactctg tttgtatatg gggagggatc cggatgaccg atatcgagat
     1441 cgcgcacagg tgcgcgctga aaaatatttc cgagatcgcc gcggctgcag gcatcggcga
     1501 agcatacgtt gagccttacg gcagacacaa ggcgaaagtt tctctcgacc tgctgcgcga
     1561 aaaggccgcc gcgccggacg gaaagctcat tctcgtcacg gccatcacgc cgacacccgc
     1621 gggcgaggga aagactacga cctccatcgg ccttaccgat gcgctccgcc gctgcggaaa
     1681 aaacgcgctc gccgcgctgc gcgagccgtc gctggggccg gtgttcggca tcaagggcgg
     1741 tgcgaccggc ggcggctatg cccagatcgt cccgatggag gacataaacc ttcattttac
     1801 cggcgatttt cacgccatcg gcgctgccaa caacctgctc gcggcgatgc tcgataacca
     1861 catccagcag ggcaacgccc tcgggatcga cccgaagcag atcacgtgga agcgctgcgt
     1921 cgacacgaac gaccggcagc tgcggcacgt tgtggacggg ctcggcggca gagcgaacgg
     1981 ctgcccgcgc gaggacgggt tcgacatcac cgtggcaagc gagatcatgg cggtgttctg
     2041 tctcgcggag agcctttcgg atctcagggc gcggctcggc cgcatcatcg tggggtatac
     2101 ctatgacggc gcgcccgtta cggcgcacga tctcagggcc gagggcgcaa tgaccgcgct
     2161 gctgcgcgat gcgctctgcc ccaaccttgt ccagacgctc gagggtacgc ccgtgctcgt
     2221 ccacggcggc ccgtttgcga atatcgccca cggctgcaac agcgtgctcg ccacgcgcat
     2281 ggcgcaaaag ctcgcggact ataccgtcac cgaggcgggc ttcgccgccg atctcggcgc
     2341 ggaaaagttc tttgacatca agtgccgtct ggcagatctg cgcccccatg ccgtcgtgat
     2401 cgtggcgacg gtgcgcgcgc tcaaatacca cggcggcgcg gagaaggacg cgctcggcac
     2461 ggaaaatctc gaagcgcttg aaaaaggcct tccgaacctt ctgcgccatg cggacaatat
     2521 caagaatgta tttggccttc cgtgcgttgt ggcggtgaac gttttcccga cggacacgga
     2581 agcggagctc gcactcgtgg cggaaaaatg cgccgcgctc ggtgtgaagg ccgtgcgcag
     2641 cgacgtgtgg gcgcgcggcg gcgcgggcgg cgaagcgctc gcggcggagg tcgtgcggct
     2701 ctgcgaggag aaaaacgagt tccgcttttc ctatgatctt gacggcacga tcgaggagcg
     2761 gctggacgct ctctgccgga acgtgtaccg cgcggacggc gctgttctca cgccggaggc
     2821 aaaaaagcag gccgcacggc tcacggcgct cggcttcggg gctctgccgg tgtgcgttgc
     2881 caaaacgcag tacagctttt cggacgacgc ggcaaaacgc ggcgcgccgg agggcttcac
     2941 tgtgacggtg cgggagatcc gcgtctcggc gggcgcgggc tttctcgtcg cgctcacggg
     3001 caatattatg acgatgccgg gcctgccgaa gtcacctgct gccgagcgga tcgacgtgga
     3061 cgaaaacggc gtcattacgg ggatgtttta agaatggaaa tgctccgcaa aagcgtgaat
     3121 gattttaccg cctcgctcgc gtccaaggcg tccgtgccgg gcggcggcag cgcaagcgcg
     3181 cttgccggat cgctcgccgc cgcgctcggc ggcatggtgg gggagctcac cgtcggaaag
     3241 gaaaaatacg ccgccgtcga gccgatactg cgtgagctgc tcgacgaggc ggaaacgctc
     3301 cggggccggc ttctggactg cgtggaaaag gacgccgcgg cgctcgcgcc gctcgcaaag
     3361 gcctatgcga taccgaagga cgatccgcag cgggccgaaa cgctcgaggc gtgtctcacc
     3421 gccgccgccg cgccgccgat ggaggtgctg gagcttaccg ggcggacggt ggagctgctg
     3481 cgcggctttg ccgacatggg aagccccatc gcggtatcgg acgccgcggt cggcgctgct
     3541 ctcgctctcg cggcgcttcg cggcgcggag atcaacgtgc gcgtcaatac gcggctgatg
     3601 caggatcggg agcgcgccgc cgcgctcgac gcgaaggctc acgcgctcgt ggataagtac
     3661 gcgcggcagg ccgaaaagat ctataacgat gtatacggga ggctcgcgcg atgagcatgc
     3721 ttctccgcgg cgcgcccgct gctgccgcgc tcaacgaaaa aacggcgcag ctcgtatccg
     3781 gcctgcacga aaagggcgtc gtcccgacgc tcgccatcgt gcgccttgga gcgcgcgagg
     3841 acgatctctc ctacgagcgg gcggcggtga agcgctgtgc cgccgtgggc atcgaaacgc
     3901 ggtgcgtctc tctgccggaa aatgtttccg gcgaagcgct cgaagaaacg ctgcgcgccc
     3961 tgagcgccga tgcatcggta cacggcatac tgctgctgcg cccgctgcct gcggcgctgg
     4021 acgaggcgcg catgctgcgc gccatcgcgc cggaaaagga tgtggacggc gcggcggagg
     4081 gctctctcgc cgcggtgtac gccgggacgg acgagggctt cgccccctgc acggcgcagg
     4141 cggtgctgga gctgctggac tattatgaaa ttcccatcga ggggaagcgc gccgtcgtgc
     4201 tcgggcggag cctcgtcgtc ggacgtccgg cggcaatgct gctgctgcac cgcggcgcaa
     4261 cggtgacgct ctgccactca aagaccgaaa acgcggcggg ccttgcgcgc gaagcggata
     4321 tcctcatcgc cgcggccggg cggcgcgaga gcgtcggcgc ggcgtatttc cgcgccggac
     4381 agacggtcat cgacgtcggc atacactata acgaagagac gcagaagctc tgcggcgatg
     4441 ttgctctcgc cgaagcggat ggcgtcgttc gcgccgttac gcccgtcccg ggcggcgtcg
     4501 gcgcgctcac cacggcggta ctggccgcgc acacggcaaa agccgcggcg cgccggataa
     4561 aataaattca attcagaaaa cacatgacag agtaggtgca aagcgtaaag tgccgcggga
     4621 tgggatgttg tccgtggacg aagcggcggg agccgcgcgg ttttgctccg catccgctgc
     4681 cacccgagga taccgctcct tccgtggcaa ctgtcacgaa aggagtcttt ttacgccatg
     4741 aaaacaagaa aactcgccct gaacgccgtg ctggcggcaa tgtgcgccgc gctcggcgcg
     4801 ctcgcgctcg atctcaacag catcaagatc acctttgaga gcttccccat cctgctcggc
     4861 gcgctgctct tcggcccgct ggacggtctc gccgtcgggt tcgtggggac gcttttgtac
     4921 cagcttctgc gctacggcgt ttcggtcacg acgccgcttt ggatattgcc ctacgcgctt
     4981 gcggggctcg tgacaggctt ttacgccaag cgccgcggct tttcgctcac gaccggacag
     5041 acggtcggca tcgtcgtggc cgccgaggtg ctcgtcacgg ggctcaacac gctcgtcatg
     5101 tacatcgacg caaagctcta cggctactgg tttccgggct tcatctcggc catgctgctg
     5161 ccgcgcggcg cggtgtgcgt tgtgaaagcc gttgtgttcg gctttgtact gccgaagctg
     5221 tgcgcacgcg tgcgccgcgc gctgcccgga gagggggaga aaacacatgg agcttaacga
     5281 agcgctcgct tttatccacg ccaccgactg gaagggaagc cgcctcggcc tggagcggat
     5341 gcgcgagttg atgcaccggc tgggaaaccc gcaggacagc ctgaaattta tccacgtcgc
     5401 cgggacgaac ggcaagggct cgacgagcac gatgcttgcc tcgatcctga cggcggcggg
     5461 gtataagacg gggctttaca cctccccgca ccttgtgcgc gtgaacgaac ggttccgcat
     5521 caacggcgcg gacatccccg acgcggcgct ctgccgcgcc gccgaagccg tgaaggaggc
     5581 cgccggcggc atgtccgacg cgcccacgga gtttgaaatc ctcaccgcga tgggcttccg
     5641 gcatttcgcg gcggagaaat gcgatgccgt ggtgctcgag gtcggtctcg gcgggcggct
     5701 cgacgcaacg aacgtgatac cctcgcccga ggccgccgtt atcacgaaca tcggcctgga
     5761 gcacaccgcc attctcggcc atacgctccc ggagatcgcg cgcgagaagg ccgggatcat
     5821 caagcccggc acgacggccg tgacgtaccc ggtaaacgcc gccgtggacg cggtataccg
     5881 ggagatatgc gccgaacgcg gcgcggtgtg gcgcgccgcg gcgtttgacg cgctgcagga
     5941 gcgcgcccac tcgctcgccg ggcagcggtt cgactataag gacgaaaaag acctgacgct
     6001 gcatctgctc ggagagcatc agctccgcaa cgcggcgctc gccatcgaaa cggtgaacgt
     6061 gctgcgcgaa aaggggtgga gcatcccgga gagcgcgctg cgcgagggac tttccgcggc
     6121 gcgctgggaa gcgcggttcg agctgctgcg ctccgatccg cccgtcatcg ccgacggcgc
     6181 gcacaacccg cagtgtgttg aagcgctcgc acgcagtctg gacgtgtatc tgccggggca
     6241 gaaggtcgtc tttctgctcg gcgtgctcgc ggacaaggac tatgagacga tgctcgcccg
     6301 gctgctgccg tacgggaagg agtttatctg cctgacgccg gactcgcccc gcgcgctggg
     6361 tgcggacgag ctttgcgcgt atcttcgcgc gaagggcgcg cccgcggttt cgtgcgatac
     6421 ggcagcggag ggcgtgcgcc gcgcgctgga ggaaagcggc ggggaaacgc ccgtcgtcgc
     6481 ctgcggctcg ctctatctca tgggcgagat ccgagaggca gtgaagagcg gcagataaaa
     6541 aggcagggga ggggccgagc ccctcccctg cgggtatgga gagcttctct gcataaaaga
     6601 ggggcgttgc gctttggcgg cgggtctggt atagtggata aaacggtgtg
//
LOCUS       DAWAAE010000114         6641 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5005_length_6641_cov_2.705284, whole genome shotgun sequence.
ACCESSION   DAWAAE010000114 DAWAAE010000000
VERSION     DAWAAE010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5005_length_6641_cov_2.705284"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3043..3052
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1252 a         2068 c         1924 g         1387 t
ORIGIN      
        1 ctgcaataca acggaatagt cgtgcttcgg cacgctttcc ttgaagtttt cgatgaccgt
       61 atagcgcgtg gcgtcggacg ccgtgtccgc cgcggaaacg ttccagtcaa aataagcgta
      121 gcccatgtcg ttcagatcgt tcgtaagacg cgtcatgatg ccggggttaa aatggctcac
      181 ggtgttcgag ctgccgccgg ggaagcggac gagctttgca tactcgcccg tctgctcaaa
      241 tatgatctcc tgcatccggc agagatcggc aaagtacgcc tcttcactct gatagatggc
      301 gttatagtcg tgcgttgcgc tgtggatgcc gacggagtgg ccgcgccggg cgatctcgcc
      361 gatgcaccag gtataatcct cattgcagtt cgtcacgaaa aacgtcgcct tgacgccata
      421 ggcatccaga atatccaaaa gatgctccgt attggcggaa ggcccgtcgt caaacgtgag
      481 atagaccgtt cccgcctccg gcgtgaccgt atcgggccgg tccgccgccg tgacgatcac
      541 gcggcgcgtg atggtcgagc tcagtccttc gctgtccgtg acggagtagg tgacgctccc
      601 ctcgccggtg tggaaggcgt ttacgtccgc ggcgcattcg atcttcgcgg tgatatcacc
      661 gtcgcggtca tcgatggccg tacagccggg atcttcgtat ccgcccggct gtgccggata
      721 aaagagcgga tcgtcgccga tgagcgtgag aacgggcgcg gcgttcggcg cggctgtcgg
      781 ctccgccgtc gggacgggtg ttccggccgg cgccgcggtc gcgcccgcca tggcgggcat
      841 tgtatctctc tggtttacat aatataaacc cagtccgcag aatgcggcaa gcgcgcagcc
      901 cgcggcgatg agcgccagac cgagcttttg atgtttctcc atacctgttc ctttacttct
      961 ccccggtgtt ttccgcagcg cggcgcagcg cctccgccac ggcgtcggag cgctcgcggc
     1021 ggttgagccg ccggagcgtt tcgcggatct cttccccggc ctgcatcact tccgtgtgga
     1081 agctgtcggc cgagaggcgc agcgcgccct gaccaaggac aatgagctgt tccatgccgt
     1141 cagaggtcgc aacgcgcacg cggtggtctt tggcgatctc atgcgaggtt ttttcgatat
     1201 aagcgtccgc cgtttccgct tccctcgtat agacaacgtg gatattatta tatgccacga
     1261 tctcttcctt gtgaaacggg acgcggtagg catcaaatac cagaatgacg cggcactggc
     1321 gcacgccctg atagttgcag agaagatcca tcagaagctg gcgcgccgca tcgagatggt
     1381 cggcggcaac ggctttgagc tcgtcccatg caaagattat attgtacccg tctacgagca
     1441 aatattccgg cccggcgggg atcggctttg ctttcggctg cggaaccggt ttttcacgct
     1501 cagaacgagg cacgaacgcg cggttttgct tcactgcgcc gtaggttcgc tcataaatgg
     1561 cctgcaattc cttatcgagc gccgcgcccg tgcggggcgc ggacgggccg cgcggcatgc
     1621 tctcctgtgc gggcgtggtt tccttctccg cccggaggca gctttccaga tgcatatgct
     1681 gctcgacctc gttccagcgg acggtatagc ccgcgccgtg ggagcagaaa acggaatccg
     1741 ccgtattggc aacatcgcgc tccggatcgt atccgatctc ttctacgaca cgctgcgtat
     1801 cgcggcaggg ttcatagccg cgcagcgaga gcgaaacgcg cccctgcccg cgggaatacg
     1861 ccgtcacctc gcgcgggtaa ttcgcgcact ctgccaccgg cgcggcagcc atgaggacgg
     1921 aaacaccgtt ttccgtttcc ggcggatcga accggccgcc catgcgctga atgtcggcca
     1981 tggcgcggcc gacacactcg ttcggcagct cctgccggag atcataccac ggctcgagaa
     2041 ggatgctctc ggcacgcatg agaccctgcc gcacggcgcg gtaggtggcc tggcggaagt
     2101 cgccgccctc ggtatgcttc aggtgcgccc gccccgcggc gagcgtgatc ttcatatcgg
     2161 tgatcggtga gccggtcaac acaccgcggt gctctttttc cgcaagatgc gtcaaaatga
     2221 ggcgctgcca gttgagatcg agcgcatcgg tcgagcattt gctcgcaaag acgaggccgc
     2281 tcccgcgctc gagcggttcg agcaggaggt ggacctcggc atagtggcgc agcggctcat
     2341 aatggccgac gccctccacg ggtgcggcga tcgtttcacg gtagaggata ccgccctcgc
     2401 cgaacgtgat ctccagcccg aagcggctgc ggcagagacg ctcgagtatc tccagctgca
     2461 cctcgcccat gagctgcacg cggatctccc gcgcggcctc atcgtacacg acgtgcagca
     2521 gcgggtcctc ttcctcgagc agacgcagct tttcgagcac cgtgtgcgga tcggccttct
     2581 ccgcgtggac gcggtaagtg aataccggct cgagcagcgc agcgctcttt cctttttccg
     2641 cgccgagcgc gtcgccgctg tgcgcggcgg tcagccccgt tacggcgcat accgttccgg
     2701 cctttaccgc atcggtcagc tcgtattttt ccccggagta gagacggatc tggtggatct
     2761 tctcctcggt ctcgccgctc tccgtcggaa cctttaccgc agcgcgggcg cgaagttctc
     2821 cgcccgtaac acgcagccac gtgagccgcg cgccctgcgc gtcgcggctg atcttataca
     2881 cccgcgcgcc gaaggcgtcc ccggcgggct tgaccggcgc atagagcgcg agcgtatcga
     2941 gcagcttttc cacgccgtcg agccgaagcg ccgcgccgaa cagacacggg aagagctttt
     3001 cctccgcgct caggcggcgg acttcctccg gcgggatcgt ccnnnnnnnn nngaagctct
     3061 cgagcgccgc ttcgctctcg gccgcagcct gttccatcca gtccgtgccg gaaaagtccg
     3121 cgcactccgg tgagaggagg gagcgcagct cctgcatgcg cgcagcgcgg ttcacgccgg
     3181 ggagatcggt tttatttata aaaaggaaag tcggcacatg gttccggcgg agcagccgcc
     3241 agagcgtgcg cgtgtggccc tgcacgccgt ccgtgccgct cacaacgagg atcgccgcat
     3301 cgagcaccgg gagcgtgcgc tccgtttcgc cggaaaagtc cacatgaccc ggcgtatcga
     3361 gaagggttac ggcgcggttt ttccatgtga gccgcgcctg cttggaaaag atcgtgatgc
     3421 cgcgctcgcg ctcctgcggg tcagtgtcga gaaaagcgtc cgcatggtcg acgcggccca
     3481 gacgccgaag cgcgccggag gtatagagaa gcgcctcgga gagcgtcgtt ttcccggcgt
     3541 ccacgtgcgc aagaataccg atgcacaaag gggacatttc cgtatttgat ttttccgcca
     3601 tacagcgttc ccctttctct tatttcagcg tcgtattata gcacgaaagg caggattctc
     3661 caatagattt attatttcgc aaaatcattt gaaacgtttt ctacaaaaac gcttttcaaa
     3721 atttgacaaa atgactaaat gggtgttttt tgcaaaatgc ttttctgttt ggattgacaa
     3781 agcaaaatcc gatgactata attatcatca ttcatccggc aagtttaaac aaggttgcag
     3841 caaagatcaa accgtgttta accattaccg gagcattacg aaatggagga tccgaaagtg
     3901 aaaaagtatc tggcactgct tctggcgctg gtcatggtgt tcgcttttgc tgcctgcggc
     3961 agcacggccg aaactcccgc cgccaccgaa gcccccgctg cggaacccga gaccccggcc
     4021 gaggcggccg aggccgatcc catgaaggat ctgatcgccg cggctcaggc cgagggcgag
     4081 ctgaccgtct acggctcctg cgaggaagaa tacctcgccg ccgcctgcca gcatttccag
     4141 gaactgtacg gcatcaaggt caactaccag cgtctgtcca ccggtgaggt gcaggcgaag
     4201 atcgaagaag agaacggcaa cccctccgcc gacgtctggt tcggcggcac gaccgatccc
     4261 tacaacgttc tcgccaagga agacctgctt gagccttacg ccgcccagaa cgcctcccac
     4321 ctcatcagcg atatgtacaa ggacgctgag ggcaagtggt acggcattta caagggcatt
     4381 ctcggcttca tggtcaacac cgacgagctc gagcgtctcg gtctcgaggc tccggcggac
     4441 tggcaggatc tgctcaagcc cgaatacaaa gacctgatct ggctctccaa ctacaacacc
     4501 gccggcaccg ccaagctggt cgtcaacacg atgatccaga agtacggcca tgacgaaggc
     4561 atccagtacc tcgtagacct cgacaagaac atccaggttt atacgaagtc cggctccggc
     4621 ccgtccaaga acgtcggcat cggtgagtgc gtcatcggca tcggcttcct gcatgacggc
     4681 atcacccaga tcgtcgataa ccagtacggc aatatcgccc ttgtgatccc gtccagcggc
     4741 acctccttcg aggtcggcgc gaccgccatg ttcaagggcg cgaagcaccc caacgctgcg
     4801 aagctgtgga tcgagtacgc tctgtctccc gagtgcgttg agctcgctgc caagaacggc
     4861 tcttaccagt tcctggtcat cgacaacgcc acccagccgt cgcaggctgc ggagttcggt
     4921 ctggatcccg acaacgtcat ggaatatgac tttgaggatg ccaagaacaa caccggtact
     4981 tacgtcgagg aagtcatggc tgccctgagc aagagcggcg ctgacaccgg cgaggatcgt
     5041 ttcaagactt cgtaatatcc cctaatgcca aggcggcggc ttctgccgcc gccttgttcg
     5101 taaaccgtcc gatggatcga ctgtcattat gaatccgcac gccggatgcg cgccggttca
     5161 taatggcagc gggtctgttt taaagaaacc gaaagagtca ggagggattc cgcctatggc
     5221 aagaacacag ggcgcggcgc tggaccggaa gaaactgatg ggtgacccca ttctggtcac
     5281 gaccatcgtc gtgctgatca cattcctcac gcttttcatt ctctatccgc tcgccatact
     5341 gctggtggac agctttgtgg gcgacggcgg gttcagcttc aatgtgttca agcgcatttt
     5401 ccagatgccc acctttaccc atgccatcac aaacacgctg aaagtcggct tcctcgtcgg
     5461 aattctgtcc acgctggtcg gtctgctgtt tgcgtatgtg gaagtgtacg tccgcatggg
     5521 caaattcacc ggtggacttt tcaaggtcgt atccatgctg ccggtcgtgt ccccgccgtt
     5581 cgtgctcagc ctttcgatga tcatgctctt cggcaaggcc ggcatcatca cccgcttcct
     5641 gctgaagatc tacgataaca gcgtgtacgg cttctggggc atcgccattg tgcagacgct
     5701 gacgttcttc ccggtgtgtt acatgatgct caagggactc ttgaaaaaca ttgacccctc
     5761 gctcgaagaa gccgcccgcg acatgggcgc ttcgcgctgg aaggtgttca cgagcgtgac
     5821 gttcccgtta ctgctgccgg gtctcggcaa tgcgtttctc gtgacgttca tcgagtccat
     5881 cgccgacttt gccaacccca tgatcatcgg cggctcctat gatacgctcg caacgaccat
     5941 ctatctccag atcaccggcg cttacgacaa ggccggcgcc gcggcgatgg cggtggtgct
     6001 cctctgcatt acgctcgcga tgttcgccgt gcagaagtac tatcttgagc gcaagaccgc
     6061 cgccacgctc accggcaagg catcccgcgg ccgtatgctc atcaccgacc gcagcgtccg
     6121 agttccgctc acggtgctct gctcgctcgt ggcgctcttt gttatcatga tgtacatttg
     6181 cgtcccgatc ggtgcctgct tcccgacgtg gggctacaag ttcttcccgc tcacgggcaa
     6241 gtggttcaag ctcgtattca cgcgctacca cggcttccag gcgttccgtg attcgtttat
     6301 tctcagcttg atctccgcgc ccatcacggc gcttttgagc atgatcatct cgtatctcgt
     6361 cgtcaagcgg aagttcaaat ccaagggctt tatcgaggcg gtctccatgc ttgccatggc
     6421 ggtgccgggc accgttctcg gcgtcggcta tatccgcggc ttctcgggcg gcctcttcca
     6481 caccggattc atgcaggggc tttacggcac gggactcatc ctcatcatcg tctttgtcgt
     6541 ccgctctctt ccgaccggca cgcgaagcgg catctccgcg ctgcggcaga tcgacaagtc
     6601 catcgaggaa tccgcctacg acatgggcgc ggacagcttc a
//
LOCUS       DAWAAE010000115         6549 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5077_length_6549_cov_3.348630, whole genome shotgun sequence.
ACCESSION   DAWAAE010000115 DAWAAE010000000
VERSION     DAWAAE010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_6549_cov_3.348630"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          956 a         2154 c         2243 g         1196 t
ORIGIN      
        1 gcgtccaaaa gaacaaaaat ccctcgaaaa tccaaggctg tccgacgtgt gtgcccttgt
       61 taatcggcgc ttgcaacaaa aagctgtcag aagccgaacg gcattttgcc gccgccgcgc
      121 tggaagcgct tcattttctt gccggagagc tggcgcgcca tcgtctgcat catttcaaac
      181 tgcttgagca gacggttgat gtccaccacg ccgacgcccg cgcccgcggc gatgcgccgc
      241 ttgcggctgg agttcaaaat cgaggggttc tcgcgctcgg cgggagtcat ggagaggatg
      301 atcgcctcgg tgtgcgccat ggccttctcg tcgatcttcg caccggcgag cgccttggcg
      361 tccagccccg gcaccatccc ggcgagctcc tgcagcgagc ccatgttctt catggagttg
      421 agctggtcga gataatcggt cagcgtgaag cggttctttt tgagcttttc ggcgagctcc
      481 tgcgctttct tctcatcgac ggcggcctcc gccttttcga tgagcgtgag cacgtcgccc
      541 atgccgagaa tgcggctcgc catgcggtcg gggtgaaaga cctcgatggc gtcgagcttt
      601 tcgccgacgc cggcgaactt gatgggcttg cccgtcacgg ccttgacgga gagcgcggcg
      661 ccgccgcggg cgtcgccgtc gagcttggtg agcatcacgc cggtgatgcc gagcgcctcg
      721 tcaaaggcgg cggcggcgtt cacggcgtcc tggccggtca tcgcgtccac gacgagcaaa
      781 atctccgccg gttcggtctc ggccttgatg cggcgcagct cgtccatgag cgcctcgtcg
      841 atgtggagcc gtccggcggt atcgaggaat acgagatcgt ttccgtggcg gcgggcgtgg
      901 tcgatcgcgt gcttggcgat ctccaccggg tcgccctggc cttcctcgta tacgggaaga
      961 tcgagctttt tgccgacggt cttgagctga tcgatcgcgg cgggacggta tacgtcgcac
     1021 gcgacgagca gggggcgctt gttctggctc ttgcgcatga gcgcggcgag cttcgcgccg
     1081 ttcgtggttt taccggcgcc ctgcagaccg acgagcatca cgacgctcgg ggacttcgag
     1141 ccgatgttga gcttctggct ctggccgccc atgagcgcgc acagctcctc gttgacgatc
     1201 ttgatgacca tctgcgcggg ggagaggctc tcgagcacgt ctgtgccgac ggcacgctcg
     1261 ctgacggatt tgacaaagtc cttgacgacc ttgtagctca cgtcggcctc gagcagcgcg
     1321 agacgcacct cgcgcatcgc ttcctttacg tccgactccg acaggcggcc cttgccgcgg
     1381 agctttttga aaacgttatt gagtttgtcg gaaagatttt caaatgccat gtcccagctc
     1441 ctgtaatgcg gcggcgatct cgcgcgttaa gctctgcgca gcgccatcgg tcatggtttc
     1501 gagccgcgcg agcttttcct gcgccgcggc gagaatgtcc cgctcggcgg cgaagcgcgc
     1561 gaccagcccg gtcttctctt caaaccggcg catcgtctcc tcggcgcggc ggatgatgtc
     1621 ccacacgctc tgccgggaaa cgccctcgct ctgcgcgatc tcggcaagag agaggtcttc
     1681 gttgtaatgc aggtcaaagt actcgcgctg cttttcggtg agcagctcgc cgtaagagtc
     1741 aaagagcatc gtgcgaaaga gcgcttcttc catactctct gcctccctct gtcaaggatt
     1801 tttccctgtc accggcacat tatacccgcc caaagcgagc ctgtcaaggc tttttcgctg
     1861 acagcgggga gaaaacgcat atcgcgtgcc gctccgggga aaaatgttcc gtaaatgtca
     1921 tgctttggag gcggaaacta tggaaacggt cgtgatagcg cttgaagagc tggcgcatta
     1981 tgccgagagc acggcgcgca cgatgcccgt gacgggtgtg gtgccgcgcg ccgtggcaga
     2041 ggatcagatc cggcacgcgc tgcgagctct gcgcgaaaag cgccggcagc tggatgcgtc
     2101 cgcgccggac ggcggagcgc ggtggctgct cgacaatctc tggctgtgcg agcgggaggg
     2161 agcctccgcg ctcgccgcgt tccatgccgc cgggcggctt cgcaccacgt gcgagggggc
     2221 gctcgtcccc gcgctctgcc gggtgctgct cgcctccggc ggcgggcgcg tggacgagga
     2281 gaggatcgcg gtatttctgg aaggctttca gcggaaaaca ccgctcaccg ggcgcgagct
     2341 ggctcttctc ggcgcgtcgc tgcgcgcggc ggtcctcgtc cggctcgcgc agctctaccg
     2401 gggtgagacg ccctccggtg cggcggccgc cgtgtatttt acagcgctgc ggcacatcgc
     2461 ctcgctcgat ctggacgcgg tgctggaaga ggccgacgcc gtggagcaca tcctccggcg
     2521 cgacccggcg ggggtgtacc agcgcatgga cgagcgcagc cgccgggact accgcgcgcg
     2581 cgtcgaaacg ctttcgcggc ggaaaaataa aagcgaggcg cgcgtcgcgg aggaggcgct
     2641 gcgccttgcc gagcgcgccg atggcgtccg gcggcgccac atcggctact ggctctatga
     2701 aaatcccctc ggcgcgggcg tgtcgcggag aggcggcgcg gcgtacattc tcgcaaacgt
     2761 ccttttcacg cttgcgctct ccgcggcgag cgcgctgtgg acaaaaagcg tcctcacggg
     2821 agcgctggcg ctgctgccgg tctcggaagt cgtgaaggga cttttggacc gcgcgctgct
     2881 ccgcgccgtg ccgccgcgcc atctgccgcg gctctctctg aaagacggcg tcccgcccga
     2941 gggaaagacg gtctgcgtgc tgtcggtatt gctcacgggg gagaaggccg cagacggcgc
     3001 gctccggcgg cttgaggaat ttcgcgcggc ctcccgcggc tgcggggaaa accttctctt
     3061 cggtcttttg tgcgatcttc cggagagcgg cgaaacgctc tcgcacgccg accgcgttct
     3121 gctcgatcat gccgccgcga agactgacgc gctcaacgca cgctgcggcg ggggctttta
     3181 tcttttcacc cgcgaccggc tctacagccg ggacagcggg aaatttgctc cctgggagcg
     3241 aaagcgcggc gcgctgctgg agctttgccg tctgctcgcc ggggagaaca cgactcttca
     3301 cgtccgcgcc ggagacgcgg agaagctgcg ctccacgcgc tatatcctca cgctcgacgc
     3361 cgacacgcgt cttgagccgg ggagcgccgg agagctcatc ggcgcggcgc tccacccgct
     3421 caaccgcccg gcggtcgatc ccaaacgcgg gatcgttttt cgcggccacg gcgtgctgca
     3481 tccgcgcatc gccgtgtcgc tcgagagcgc gtaccggaac gacttcacgc gcctctttgc
     3541 ccccgcgccc ggcggcgatc cctacggctc ggacgccggg gaggtgtata tggacgcctt
     3601 ccaaagcggc ggcttcgcgg ggaaggggct catccatgtg ggggcgtatc tcgcgtgtct
     3661 gggggaacgc attcccgaag ggcgcgtatt gagccacgac gcgctcgagg gggcgtttct
     3721 ccgcggcggg tatgtgagcg acgtggagct gaccgacggc ttcccctccg gtgcggtgag
     3781 ctattttgcc cgcgcgcacc gctggatgcg cggggacgtg cagaatctgc cgtggctctt
     3841 ccggcggggc aaagcgctcc cgcccatcga gcgctggcgg cttttcgatt ccctccggcg
     3901 cgcgctgctg ccggtcggcc ttttcgcggc gctcggcgtg tggtttctct ttccgaacgc
     3961 cgtgacgcgc ctgcccgcgc tctgcgcgct gctcgtcctc ttcctgccgg tgctccggta
     4021 cggccttagc atcctctttc gcgacgggcg ggaggtgcgc ttcaaaagcg ccgcgctgca
     4081 cggtctcggc ggcgagctga cgcgcgtttt cgcgcgggcg ctctttctcg cggcggaggc
     4141 gtggacgagc gtttccgcga tcgtgctcgc gctctggcgg atgggggtct cgcaccggcg
     4201 gctgctccag tggcagaccg ccgagcagag tgagcgccga agcgccgatc tcgccggaac
     4261 gttcgccgca atgtggccgg tcgtatattt ctccgggctg ctgctcgcct tttcgcagac
     4321 gttcgccgga cgcgccgcgg cggtctgctg gatctttacg ccgctcatcg cccaccggct
     4381 caaaagagag aagaagaaga agccgctcga cagcggagag cgggagtttc ttctgcggcg
     4441 cgccgcggag atctggcggt acttccgcga aaactgcacg gcggcatcgc accatctgcc
     4501 gccggacaac gtgcagctct cgcccccggc gtcgggcgcg gaccggacga gcccgacgaa
     4561 catcgccatg gcgctcgcgg gcgctctctc ggcactcgag ctcggcatcg cgtcggagac
     4621 cgaggcgctc acgctctgcg gaaacgtcct cgatacgatc gggcgtctgc ccaagtggaa
     4681 aggccatctt tataactggt acgatacgaa aacgctcgcg ccgctcgatc cggcgtatgt
     4741 ctcgaccgtg gacagcggca atctggccgc cgcgctcacc gcctgcgccg cgggctttgc
     4801 cgcgcacggc gcgcccgctc ttgcgaagcg ggcgggagag atcgccgcgg cgatggattt
     4861 tcgcgcgctc tacgatgaga aacggcggct tttccgcatc ggccttctgc ccggcgagag
     4921 cgctccggcg cagagctggt acgatctttt agagagcgaa gagcgcctga cggcgtattt
     4981 caccatcgcc tccgggcaga tcgagcaccg ccactggcgg cagctcagcc gcgcgcaggt
     5041 gggctatcgg aggcggcgcg gcatggtaag ctggtcgggc agcctttttg aatatctgat
     5101 gccggagctt ttcctgccgc tgtaccgcgg ctcgcatctc tgggagagcg cccgctttgc
     5161 cgtatacgtc cagcggcggc gcacgttcgg cgcgaaaaaa ctctggggca tttccgagag
     5221 cgccttttac gcctttgacg ccacggacca ttaccgctac aaggcgcacg gctgcgcccc
     5281 gctcgcactc tgccgcgatc gaggcgatga gagcgtcgtc tcgccctaca gcagctatct
     5341 tgcgctctgc accgcgccgc acgcggcggt ggaaaacctc cgacgcatgg cggagaccgg
     5401 actctccggc ccctacgggc tgtgggaggc ggtcgatttc acttcctcgc gtaccggcgg
     5461 cgggccggtt tgcgtccggt gcgtgatggc gcaccatctc ggcatgagcc tttgcgcgat
     5521 cgcaaacgcg ctgtgcggcg gcgtgctgcg ccggtggttt ttcagcgacc cggccatggc
     5581 cgcctacaca gggcttttgc aggagaagat ccccgccggc ggaaaactct tccgccgcgt
     5641 gccggggagc gcgggaagag cggagcgtgc cgggagcgcc gcgccccggg ccggcgaggg
     5701 ggcggacccc ttcttcccgg acggcgcggc gctctcgaac ggcgcgtacc ggctgctcgt
     5761 gacggcgaac ggtatgtccc gcgcctcgtg cggcacgctc atcccgtacc ggagcgcccg
     5821 cacgccgtgg gaggacgcgc ccggcatcgc gctctgggcc gagagcggcg gcgtccggca
     5881 gccgctgctg ccaaagccgg gggagaaggg cgcgctgcgc cggcgcttca ccgccgggga
     5941 agcgctcctc gcagggcaga gcggggatct tttctgggag atgcgctgca ccgtttcgcg
     6001 cgcgtatccg ggcgagctgc ggcggctgcg cctgcgctcc gggaaggccg cgccggacgc
     6061 ggtcatcttc ggctttgagc cggtgctgct cggtgcgcgg gactatgccg cgcacccggc
     6121 gtttgcccgg ctggggcttt gcacatcggt gcgctccggc gtgctcatcg tgtaccgcgc
     6181 gccgcgggga aaccagagcg cgcagtatct tgcgcttgcc tgctcgggag aggcgggatt
     6241 ttcctccgac ttccggcagt ttcccgcccg cggcgacgcg ggggagttcc gggaaaatac
     6301 gggctggcag aatgagccgt atattgccgc gcgcctgccg cttccggaag ggagcgcgtg
     6361 cgaaattgcg ctcgcgctct gcgtcgccgg agacgcggac accgccgcgc gcggcgcgcg
     6421 ggagatgctc cgggagagct ccggctttgc catggctgac gcggccgccg cggtttgggg
     6481 catggagagc ggcgagagcg ccgcggccat ggaatacctc cgcgcgctca cctttccgca
     6541 gttctgcgc
//
LOCUS       DAWAAE010000116         6532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5098_length_6532_cov_3.414698, whole genome shotgun sequence.
ACCESSION   DAWAAE010000116 DAWAAE010000000
VERSION     DAWAAE010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5098_length_6532_cov_3.414698"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5744..5753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1149 a         2170 c         2061 g         1142 t
ORIGIN      
        1 gagaattctc tgccgccgga agcgttttta ctcttcggtg tgctcttccg gctgctcttc
       61 cacagtctcc gcagcgggcg cttcgggctc ttcctcgacg gtgatctcga cggtaatgtc
      121 ctcgtcgccg tcgtcctcgt cctgtgcgac gacgttctca aagcttgcgt cctcgctcac
      181 gtggaacgag cccttgagcg cgtcgatctc cgcctgcatc tcttcgatgc gcgcgttgac
      241 cgcatccact tcctcaacga gctgcgcata caggccctcg gcatcgttgt gatgctcttc
      301 gtagtacgct ttgccgagct caatgtacgc cttcttcagc gcgtccttct cccccgtcag
      361 cgaaacggtg agcttggcga gacgggcggc gtctttcgtt ttgtccatgg cagcgttagc
      421 gtattccgcg ccgcgctcgc gcaggttgga aatggtgtcc ataagagaat cgaaattgaa
      481 gttcatattc tgtttcctcc ttttagattt tatttcccgg ttatcagacg gttttatcct
      541 ccgatccgtt cggtgttttt tccttttctt ccgccgcggc gctctgcgcg cggacgcggt
      601 tcacatagag atcctccggt ctgtgcgctc tccggctctg cacgacgagg atcacgagcg
      661 cgacgaggca catgccgccg ccgatcacct gcgaggcacg cagcgccgtg ccgggaatga
      721 gcaggggcga ggtgcgcagc ggctcgagca ggaagcggat gaggccgtac cagacaaagt
      781 acagaagcgt cgcctgaccg tcgtactgcc gttttccctt ccgcgtccat atggcaagga
      841 aaataaagcc cacgagattc cagagcgatt catacaggaa cagcgggtgg acatagtacg
      901 tcgtgccgcc ggtcgtgagt cccatgcggc agaagacgtc cgtcacctcg ccgatgtact
      961 cgcggttcat aaagttggac cagcgcccga tgatctgccc gatgagcagt ccgagcacga
     1021 cgcagtccat gatcgcgccg aaggggatct tcttttttcg cgcgtacagc cacacgcaga
     1081 acgttgcgag cagcagtccg ccggggatgg ccgtgccgcc ctcccagatc ttgcaccagt
     1141 tccagagcgt ctgccatacg gtgtcgccga catagttcga ccagtggcag aaaacgtaat
     1201 acagccgcgc gccgatgatc gccgtgggaa ggccgatgag cacaacgtcg gtcagcgtgt
     1261 ccttggaaat gccgaaatcc ttcggcgcgc gccgcatgca gtagagcgcg gcgaggataa
     1321 agccgagacc gatgatcgcg ccgtaccagt aaaacgtatg gccgaagagc gtgtatgtac
     1381 cgctcgggtt gagagaaaag ctctcaccga gcatgggaaa cgtgatgatc gcgccgggaa
     1441 atgcttcggg catgggaaaa actcctttcg ctgcgggaaa atcaggcgtt gggacggatc
     1501 acggagagcg ccagacgctc acgcgtgaga ttttcgcaga ggaacgcctc gcactccgcg
     1561 agagtgatgg attcaagcac ggcgaacgcg tcgggcgcat cccagccgtc gagcttgctc
     1621 tcggcgagcg agacggcata gtcagcgaag gaatcgagcg agccgagaag ctggccgtac
     1681 ttcgcgcggc ggcagcgttc gagcgcttcc ttatccagtc cgcgcagcgc agcgccggac
     1741 acggccgcgt gcaccgcctc cgccacggcg tccggatcgc ggccctgcgc gccgaggacc
     1801 agcatggctg tcccggcgga ggaatcgtat tcgacagtgc agtcgccgtt cagaagtccg
     1861 ccggcataca gatcgttgaa aaaggccgag gccttgccga acagacactc gccggcaagc
     1921 tccgcaagga gcttctgccg cgctctcgct gtgccggacg cgggatacgg cagcttcacg
     1981 ccgagcagaa actgcggtgc ggaaacgctc atcgttttct ccgcgcgcat ccgggcgggc
     2041 agaagcgttt cgggcgcgcc gtagtcgcgc tccggcttct cgccgggcgt gtccggcagt
     2101 atctcgcgcg ccgccgccgc gatcgcctcg gggtctacat cgccgacgca gcagagcacc
     2161 atgtttgagg gatggtaaaa gacgctgtgg cagtggtaga gcgtttcggg cgtgatctgc
     2221 gcgatgctct ccacggaacc ggcgatgttc tcgcggatgg ggtggtggtc gtagagcgcg
     2281 ctcaaaagct ggaaaaatcc ggcatagccg gggctgtcct cgcccatgca gatctcctga
     2341 ccgatgatgc cctgctcctt ggcgacgctc tcctcggtat agtacggcgt ggaaacgtag
     2401 gtgagcagca gccggagatt ttccatgaag ttttccgaac ccgtgaaata atacgccgtg
     2461 tggccggtgc cggtgaaggc gttggggctc gcgccgagcg cggcgaagtc cgaaaggaca
     2521 ttgcgtccgt ccggcatatc aaacatcttg tgctcgagaa aatgggcgac gcccgcgggc
     2581 gtatcgatcc actcgccgtt caccttgaag cggcggtccg ccccgccgta gcgcgtggcg
     2641 aaaaaggccg cgtttttgcg aaagcccggc ttgaccgcga cgagaacgga aagcccgttc
     2701 ggaagaactt cctcatacag cgtttcgccg agattgggat attcgtgctt gttcattcgg
     2761 tctcggcctc ctctccgcgg agaaagtata cggcgtccag ctccacgccg cgcgcgatag
     2821 cggcaacatc ctccgccgtg acagtctcgg cgagcgccgc gatatcgtcg ggcgtggcgt
     2881 ccgtcccctg cacggtctct cgcagataga agtccgcgag atagtacggg ctgtccgcgc
     2941 cggtgcggag atcgttcgcc acgctgcgcc gcgccgcggc gagctcggcc tccgagatat
     3001 cgcccgcgcg caccgcgtcg agctgagcga ggatctcgct taaggcttcg tcgtacttat
     3061 cgacctcgat gccggagagc actgccataa tgcccttgag cgtatcgatg ctcgaaaagg
     3121 cgtagtagca gagcgagagc ttttcgcgga cattctgaaa gagctttgag gtgacgcccg
     3181 cgccgtacac ggcgttgaac acggcgagct tcgccgggtc gggatcgtcc atgcactcgc
     3241 cgagccggaa gccgacggcg agcacgccct gcgagatctc gtcctcctcg gtaaagtacc
     3301 ggggcttttc ctccacggcg ttcatacgga tatcggtacc gagatccagg tcgatctcgc
     3361 cgcgcggcag cagcgcgaac gcctcgcgca gcatagccgc cacttccttt cccggacggc
     3421 tgccgcagta gaaaaactcc atggggcaga cggccagaag ctccttatag tgcttcgtga
     3481 gcttcacata gtggatgctc tcggcctcgt ccgccgtgcc gtaagcgccg gtagcgtagt
     3541 cctcgtaggc gcacatattc tcccgcaggc gctggaacgc ccagctgcgc ttgtcatttt
     3601 tcacgcgctc gatgcgctcg acgagctgtt cgcgctcgga atcgacgtac gcgggcagaa
     3661 acagcccgcc gcgcgtcgcg ggggatagcc acagctcgct gagcagggat atgacctcgc
     3721 cgagcacgtc cgtcttgccg gggagaaagg catcctcggc aaagcccgca tagaagccga
     3781 cggcctggat ctcgccgagc ttccgcaccg atggctcgat ctccgcgccg taaagcgcgt
     3841 cgagccgctc ggaaatgcgg cgcatatccg gcagcgactt tgtgccgcgg cgcagcacat
     3901 acggcagcac ggcgttttcc gccgccgttt cgcggtgcag ctgtgtgaga agataggcgg
     3961 tcatacagcc ggttttgaat ttgtcggtct gcaccgccgt cagcgatacg caggggaaca
     4021 gctcttcgcg gaagtattcc atacggtcac gcctcttttc ctttgaatat acaaagatag
     4081 tatagcacgt ttttcccccc gttcatagcg caaatgtgaa ttttttcctc aaggctccgc
     4141 gccgtacagc tcacgcagca gccggagata cccccctgcc gcgcccgtat accagctcca
     4201 ccccgcgcgc ccttcctttc cgggcgcgta cgcgatatcc gccggaagga cgaacggctc
     4261 cgcgccgtac cccggcgcgg tgagcgagag cgccatgtcc tccgcgagcg cggccgcttc
     4321 ctcccgccgc ccggcgcgat gcagcgccag cgcgagccac accgccgcgt gggtgtactg
     4381 cccgccgttt tcccgaaagc ccggcccgta ggtcgaaaca taccccggcg cgcgctcctc
     4441 cggcagaaac gccggagcgt agatgcggat aagccgtctc tccctgtccc ggagcccggc
     4501 gagcgccgcg gtgagcgccg tctgcacgcg gtctctgtcg gcatagggcg aaaaggcggc
     4561 gaagctctgc gccacggagt cgatgcggca cgccgccgcg ccggacgcgc cgagcggcgt
     4621 gccgtcggca taatacccgc gcagatagtg atcggtgtcc cacgcctcgt tcgccgccgc
     4681 gccgagcgcg gccgcggcgg tctcgtagcg atccgcgccc gattctccaa gtttcgtgag
     4741 cagagcagaa aaatcgtgga acacgccgga ggcgaaccac gtgagccaca cgctctccgc
     4801 gctctcgccc atggcgtcaa agccgtcgtt ccagtcgccg ccgcccatcc gcagcaggca
     4861 gtgctctccg ataccgcgcc ggagcacgag cgccgcggcg cggtggcagt ggtcaagcac
     4921 cgtgccggtc tcgccggaga gcgacggcgt ttcgtaccgg ctgtgctcgc ctcgcgcgag
     4981 cggcggcgaa gtaagatacg gcgcggtttc cgcgcagagg ctatcgtcgg cggtcgcgcg
     5041 cacatattcg cacactgccc acggcagcca gagaagatcg tcgctgcagt cggtgcgcac
     5101 gcccttgtcg atctcgccgc cggtcggatg gtaccagtgc tgcacgtcgc cctccgcgta
     5161 ctggtgggcg cagcacgcaa ggatgtgcgc tctcgcgccc gcggcgtcga cgggcagaag
     5221 gttgatgcgg tcctgcagct gatcccggaa gccgaccgcg ccgccgctct ggtagaggct
     5281 tcctctgccc agcacgcggc agcagagcgc ggcatacgcg ctccacggcg cgagacggcg
     5341 cacgctctcc ggcatggcgg cagaatactt ttcgccccgc acgcgctgcg cccaccacgc
     5401 ctttgtcttt gccagcgctt ctctggcccg cgcgatgtcg agcagcgcgg ggacatccgc
     5461 cgtgccgagc agcagcaccg cctctttttc cagccggaac gtcccggcca acgccgggaa
     5521 gccgcggcgg agagcgccgg agccctcgcc gcagaggaag cgtgcgtccg ttccggccaa
     5581 gacccacggc gcgctgcacc gcagcgtcac gcgcacatcc ttatatgggc agcgcgggtt
     5641 ttccgcgcga agggcgctcc cgtcgcgcca aacggacgcg gaagccgcgt cctctttctc
     5701 cggcgcaagc tgcacgggtg caaaatacgc catattgcac ggcnnnnnnn nnngaagcac
     5761 acgcaccggc gtatcgggca caatgaacgc cgtgaccgaa atgcggttcc cgccgatagt
     5821 tctctcccaa cgggcatagc cgaagccgaa cgttacgctg cacgcgccgc catccgaaaa
     5881 gagactctcc gcgccgtgct ccgtttcgat ccacagccgt tccgcgcccg ccgcggcgag
     5941 cgcgtcgccc gaccacggga cgatggggca ctcccgggcg ttttcgtacc agagcgccgc
     6001 cgggccgcag tccgccgcgg cgcagccgag accgccgcca tagagcatat ttgcccatgc
     6061 gcgcggcggc agcggcggcg tttgaaagct cacctccccg gtgtcggtat gggataccgg
     6121 cggcgtaccg gtctctttct ccgccgccgg gggaaggcgg agagcacgcg cccgcaccgc
     6181 cggaagggac gtccccgcgg cgtcgccgaa aagagcagcg gcagcaagaa gcgcctcgcg
     6241 ctcgccgcga aaggcgaagt acaccccgcc cggcagagag agcgtttcgg aaaggcgcat
     6301 tgcctcgagc gccgtttcaa tcgccgcgcg ctgctcgttc cggtaatccc cggcgggcgc
     6361 ggtgagaaac acgagatcgt actccacgcc gcacgcgccg agcagcgcgt gccgccggag
     6421 ctcccggagc gcggcttccg tgccgttttc cccgccgtcg aacgccatga cggggagatc
     6481 gccggaaatg ccgagccgcc agagggcgtc ccgcgtcccg cccggcaggg cg
//
LOCUS       DAWAAE010000117         6482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5144_length_6482_cov_2.861522, whole genome shotgun sequence.
ACCESSION   DAWAAE010000117 DAWAAE010000000
VERSION     DAWAAE010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_6482_cov_2.861522"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1699..1728
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5653..5662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1185 a         1937 c         1978 g         1342 t
ORIGIN      
        1 cggatgcagt catagccata gacctcggcg gcctcgcgca cggtgaagaa attgccgagc
       61 gacttggaca tcttgcggtt atccacgttg atgaagccgt tgtgcatcca gtagttgaca
      121 aacttgcagc cgttcgcggc ctcagattgg gcgatctcgt tttcgtggtg cgggaacgtc
      181 aggtcgctgc cgccgcagtg aatgtcgatc gttttgccga gatagcggtt ggacatggcc
      241 gagcactcaa tgtgccagcc gggacggccc gcgccccagg gggaattcca gctcggctcg
      301 ccgggcttcg cggccttcca gagcgcaaag tcaagcggat tttcctttac atcgttcacg
      361 tcgatgcgtg cgccggactg cagatcctcg atcggctggt gggagagctt gccgtatccg
      421 gtaaacttga gcgtgcggta gtacacatcg ccgttgacct cgtaggcata gcccttttcg
      481 accagagtct tgatgatctt gatgatcggc ccgatgttct cggtcgcctt gggatgcacc
      541 gtcgccgggc ggacgccgag cgccgtcacg tccttccagt actccgcgat atacttttcg
      601 gcgatctgat cggaggtgac accctcctca ttcgcgcgct tgatgatctt gtcatccaca
      661 tcggtaaagt tctgtacgaa cgtcacctca tacccgcggt actcgagata ccggcgcagc
      721 acgtcaaaga tcacgatcgg gcgggcgttg cccacgtgaa tgtagttgta gaccgtcggg
      781 ccgcaggcgt acatcttcac cttacccggc tcgatgggct ggaactcctc cagctgcaag
      841 tccatatcgt tgaatatttt cattgtattt cctccccaac gggtgatatc tcaaatttgt
      901 tcgccgatga gctctccgtc atcgacggaa tcgatgcgca cgcgcaccat gtcgccgggc
      961 gctgcgccgc cggagaaaat cacgcggctg tcaatgtccg gggaatcgta ctcgctgcgg
     1021 ccccacggcg tgccgtccgg ctctctaccc ataacgagca catcaagctc ccggccgata
     1081 aacgtctcgg caaagccgtc catgatctcc gcctggatct ccatgcagag ttcggcgcgg
     1141 cgctgtgcct cctcggtcga acatcggtcg ggcatgagag cggcgggcgt tccctcctcg
     1201 ggggagtagg ggaacacgcc ggcgcgctcg atcttcgcct cgcggagaaa atcgcacagc
     1261 tcttcaaatt ccgcttcgcc ctcgccgggg agacccgtga tgagcgacgt gcgcagcacc
     1321 agaccgggga tccgctcgcg cagctttttg aagagcgcgc ggatctcctc tccagtgccg
     1381 cggcggttca tgcggcggag gatcgtatcg ttgatgtgct ggatcgggat atcaagatac
     1441 ttgacgatct tatcctcgga tgcgatctcg tcgatgagct cgtcgtcgat gagctcgggg
     1501 tagagatagt gcagacgcac ccagcggaaa tcaagtttac ataattcttt cagcagctcc
     1561 gtcaggcaaa ccttgccgta aaggtcgatg ccgtagcgcg tgatatcctg cgcaacgatg
     1621 atgagctcct ttacgccgga ggccgcgaga agacgggcct cttccagaat gctctccttc
     1681 gtgcggctgc ggaacttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggataacgca
     1741 gtaggcgcag cggttgtcgc agccctcggc aatgcggaga tacgcccagt tcggcccggt
     1801 ggatacggcg cggggcgtat cgtagagcgg cgcgctgatc gcgccgcggc gcgtcggctt
     1861 atccccggcg agcacggctt ccacaacgga tacgacctcg ctgtagctgc cggtgcccat
     1921 gatgccgtct acctccggga gctcggcgcg gatatcgtcg gcatagcgtt gggcaagaca
     1981 gccggagacg atgatcttga gctccgggtt ttcctcacgg taggaggcgg tctcaaggat
     2041 cttttcaatg gcctcggtct tggccgattc gataaaggcg caggtattga tgattacggc
     2101 gtcggctttc gcaaggtcgt cggtaaaagc atatccggcg tcctgcagca gagccagcat
     2161 ttcctcgctg tttacgaggt ttttcgggca gccgagagag atcatggcca ccgtggagga
     2221 attgttcatg gtgtgtcctt tccggataaa tagcgttccg ttgccgcgcg gatctcatcc
     2281 caggagtagc cacggcggag aagatagccc tgcgcgcgct gaaggccttc gggggagacg
     2341 tcgccgcgca gctttgcgcc gagtagccgg tcaacggtat cgtccgtttc cggcagctca
     2401 tcgagcgctg cgtcccacaa ttcgcggtca atgccgcggc ggtagagctc atcgcagata
     2461 cggcgcttac cgtagccctt ggcggcatag tgccgcacaa cgagaccggc aaagcgctcg
     2521 tcgttcacaa cgccgagacc gcacagccaa tcggcgactt cctcggcgtc ggtgtttgat
     2581 atgccctttt cggtgagctt atcgagcagc atctttcgcg atacgtcgcg gcgttccaga
     2641 attttcagcg cccgctgcct ggcttcgtcg ttcatcagcc ctcaaagtcg tcggcggaaa
     2701 tatcaatggc gcggcccgcg gcgatggcgg ccttgcgcgc ctggggcgtg agcagcttgt
     2761 gggcgttggc gcggatgtcg gcttcgatct tgtcggcgac ctcggggttg gattcaaggt
     2821 attccttgac cttgtccttg ccctgaatgc gttcgccgtt cacggtgaac cacgcgcccg
     2881 ccttgtcgat gagctcgagc ttcacgccga gatcgatgat ctcgctgacc ttggaaatgc
     2941 cttcgccgta gatgatgtca aattcggcct ccttgaacgg gggcgcgacc ttgttcttga
     3001 cgaccttggc gcgggtgcgg ttgcccacca tttccgtgcc ggatttgagc gtttcgatgc
     3061 ggcgcatgtc gatgcggacg cttgagaagt atttcagcgc gcggccgccc ggcgtcgttt
     3121 cggggttgcc gtacataacg ccgatcttct cgcgcagctg gttgatgaag atgacgacgc
     3181 agttggtctt ggagatggcg cccgcgagct tgcgcagcgc ctgcgacatc agccgcgcca
     3241 ccacgccgac gctgctctcg cccatctcgc cttcaagctc gctgcggggg aggagagcgg
     3301 ccacggagtc caccacgacc acgtccaccg cgccggagcg cacgagcgct tcggtaatgt
     3361 cgagcgcctg ctcgccggtg tccggctggg agatgagaag attatcgata tctacgccga
     3421 gtgcgcgggc atacgcgggc tcgagcgcgt gctctacgtc gatatacgcg gcctcgccgc
     3481 cgctcttctg ggcgctggcg acgatgtgca gcgcgagcgt cgtcttgccg caggattcgg
     3541 ggccgtagat ctcaacgatg cggccgcgcg gcacgccgcc gatgccgagc gcgagatcaa
     3601 gcgaaagaga gccggtgggg atcgcctcca cgttcacggc aatgtcatcg ccgagacgca
     3661 tgatcgcgcc cttgccgtaa ttcttttcga tttgagcgag cgcggtctcc agcgcttttt
     3721 tcttgtcggt cgccacagcg gcggccggag cggcggcctt tttcttttct gccatgatgc
     3781 ttccctcaca tttcttcaga atcggtagga atcggtttct ttttctgtgc gtaaatgacg
     3841 cgctcggtgc cgagcgtgtc gagcgtgcag ccaagattgt ggaatccggc ctcgagaagc
     3901 agcgcgcgca catccgccgc ctgcccctct ccgacttcaa atataatcca tccgccatcc
     3961 tttaaaacgg acttccagtt ttgcaggacg ctgcggtaaa atacaagacc gtcctcgccg
     4021 ccgtcaagcg cgagcatggg ttcccagtcg cggacggacg tgtccagtgc ttggcactct
     4081 tcctcggtga tgtacggcgg attgcacgcg ataagatcaa aattctgcat ccggagcggg
     4141 ggcggggcga gagcgtccgc ctcgagcgca acggcgcgcg gattgagccg gtgcagggag
     4201 atattcttgc gtgtaacgga aagcgcctcg gggcttatat cggcaaaaat gacgcgcgcc
     4261 ttcgggagat tgacgcccag cgcgcagccg atgcacccgc tgccgcagca gagatcgagg
     4321 atccgcggtt cgggagcatc tttcaaaatt tccagtgccg tgaaaacgag cggctcggta
     4381 tccatgcggg ggatgaggac cgcgggcgtt acgatcattt ccagcccaaa aaactcccat
     4441 tttccggcga tatacgccag cggctcgccg gaaagacgcc gctgcaaaag cttttccgcg
     4501 gccttttccg cctcgtcacc ggcgtacata tacatccgtg caatgagctg cgcgtcggta
     4561 tatccggcgc cctgcgcgag cagacgccgg gcttcgagcg catattcttc cacgccggcc
     4621 tccttgagcg cgcggcgcat ggcgagatac agctcattat aggtctttgc caatcctaac
     4681 ccctcttttt tttaccaggc gtccgagcct ttttcttccg ggagaacgag tttgagcgac
     4741 tcgatggcga cttcgagcat ggagtcgtcc ggctcggcgg tcgtcagatg ctgcacggcc
     4801 ttgcccggcg cggaggcgat gcgggcaagc gcgctgtcgc tccggcccgc ccacttgatg
     4861 atctcatagc tgatcgaaac gacgaccggc agaagcagca gatgcaggcc catgcgcgcg
     4921 agcgttcctt cggtgcgcac gaaggagaaa acaagaatgc tgataaaaat cacgatgaag
     4981 agaaagctcg ttccgcaccg cggatggaag cgcgactgta tgcgcgcgtt ctccaccgtg
     5041 agcggaagcc ccgcttcgta gcagaaaatg gttttatgct ccgcgccgtg atacatccac
     5101 acgcgcttca tatccgggat catcgagcag agcttgagat atccgagaaa gatcgcaagg
     5161 cggaaaatac cctcggcaag accgcgccat acgccgagcg gcacgagccg gttcaaaaga
     5221 ccggcgagaa acgtcggcag cagggcgaaa agcccgacgg agagcgcgat gccgagcacg
     5281 accgcggtat agagaataat cttttccgcc gtctcgtccg aaaaatgctt ctggatccag
     5341 agatcaaatt tatccggctc gccctgctcg tcctccggct gctgctcggc ggaccaggtg
     5401 atcgccttta cgccgttgac catggagtca agaaaattca ccacgccgcg gatgaacgga
     5461 tatcccatcc acgggtgctt gtccttgctg agcttgagcg gatcaacgcg ggagacaagc
     5521 gtaccgtcgc tcttgcggca gacgatggcc tgccggtcaa caccgcgcat gagaatgccc
     5581 tcaatgagcg cctgcccgcc gatgctcgtc cggaagggcg cgggggtctt ggattcctgt
     5641 accatagaaa aannnnnnnn nnactcctta tagttaagat gccttgcgcg gcagcgtcac
     5701 cgtgacgcgc acgccgccgc cggcggcgtt ggcaatatcg agcgtgccgt tgtgccgcgt
     5761 gacgatctcg tcgcacacgg caaggccgat gccgctcccg cgttccttgg aggagccctt
     5821 gaagaatttc ttcttcacct gcggcagctc cgccggcgga atgccggggc cgtagtcgcg
     5881 gatgcatacg gagacggtcg cgtcgtcgtg cgtgatgttc acgtcgatct ttttgccgtt
     5941 tttgccgtat ttgcaggcat tatcgagaat gttgaggaac acctgcgaga gccgctcggg
     6001 gtcgccgggg atctccggaa ggtcttcctc cggcgaaaca taggtcacgg cgagatcttc
     6061 gtggcggaaa agctcattat aggtataaag gcaatcctcg accagcgccg gcagctccac
     6121 cgtggaaacg tgcagcgtaa accgcccgtc ctgaatgcgc gtgaattcga gcagctcctc
     6181 gaccattttg gtaaggcgtc ccgcttcgcg ggtgatgatc tcaatgccgc ggcgcgagtc
     6241 gccccggatg gcctcgtcgt aggcgagcgt ttccgcccag ccggtgatgg cggtgagcgg
     6301 cgtgcgcagc tcgtgggaaa cggaggagat gaactcggtc tgcacacgtt ccgcctgcga
     6361 gagcttctgc gacatatcgt tgatcgcgtc ggtcagctcg ccgatctcgt cgtcgtgcgt
     6421 tttttccacc tgcgcgccgt agcttccttc ggcgatgcgg cgggcaacgc ccgtgagcga
     6481 ag
//
LOCUS       DAWAAE010000118         6371 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5237_length_6371_cov_2.824731, whole genome shotgun sequence.
ACCESSION   DAWAAE010000118 DAWAAE010000000
VERSION     DAWAAE010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5237_length_6371_cov_2.824731"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4056..4065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1213 a         1766 c         1968 g         1414 t
ORIGIN      
        1 gagctaacat agtgcagttt tcgccgcaag gcggaaacgg aacggtgttt gctcttaacg
       61 ggaaccaaat cgaagcccag cggagtgggt tcgatttgga aaggaggagc agcggagtga
      121 gcgcacggtg ttttttatgc aaagcataaa aatcgccgca agcgatacgc agcttgcgac
      181 gacgtggtgc gggtaacagg ggtcgaacct gcacgtctca cgacacgaga acctaaatct
      241 cgcatgtctg ccaattccat catacccgct tatagctttt tgatgatagc atacggcggc
      301 gggtaagtca aacccggttt tgaaaaatac cgccgctttg ccctgcggcg gcgaacagga
      361 ataattcaaa ataattcgca ccttagccgc atagtcatgc agggaaggga ggcggtaacc
      421 atctctgcct tggaaaattc cttgtggctg ctgccggggg agcttcggaa tacgctgcgt
      481 catctaccgg agaattcagc ctcagaagtt tgtgaaattc ggctccgccg cggaaggctt
      541 ccggcggtaa ctctgccgga cggagaacat ccgcttcttg atacgtccgc tgtaacggcg
      601 gccgatctcg cccgcgttct ggagcttgcg agcggcgcat ccccctatgc ggcgtcggac
      661 tgtctgcgcc gcggtttcat tacggcagag ggcggtgtcc gtgttggatt ctgcgggcgg
      721 tttccgggcg gcgagcgcgg cctttggaat cgggacgatc tcttttccgt gtgtatccgt
      781 attccgcgag aggtaaaagg aatcgggaag agattctgcg cacgtccgtt tccctccaca
      841 ttgatcctct ccccgccggg cggcgggaaa acaacgctgc tgcgtgatat ggtgcggacg
      901 ctctccgatg ggggaatgcg tgtcggtctt tgcgatgagc gcggagagat cgcagcgctg
      961 tctgccggca gctgcggatt tgatatcgga gagcggacgg acgtgctgtc tgactgcccg
     1021 aaggcgcgcg cggccatgat ccttctgcgc tgcatgtcgc cggagctcat cgcaatggat
     1081 gagatatccg agccggagga cgccggtgcc tgccgcagcg cggcgtactg tggcgtatcg
     1141 ctcctcgcca cggcgcatgc ctcagacgcc gaagagctct cccaacggga cgtttatcag
     1201 agactgctgc gtggcaaggt gtttttccgt gctatcgtta ttcgctgtgc cgatggctgc
     1261 cggagctatg aggaggttat gctgtgatat ccgcatttgg ggcgatcctg ctcatcgcgg
     1321 cgggaggtta cgccggtttc tccgcactgg aacacctgcg gacggccctc cgaagtgccg
     1381 aaacgatcct cgacgcatca cgcgatatgc gaacggaaat actctgtcac cggacacccc
     1441 ttccgcagct gctggagcga ctgggggcgc ggtatccgcg ccttttcccg gatgcagaaa
     1501 acgcatgcat gctcgttcgg gagttttcgt ttctctcggt ctggacggcg agcattcgct
     1561 gtgccgcgct gcccaaaccg ctggaagagc ctcttcttcg gctggggacg cagctctgct
     1621 ccggcacgga gccggagcag gcgctcgatg cgttggacgc ttcggtccgg ctcgtgcgcg
     1681 atgcgctgcg cgaaaaggac cgtgccgccg ttcggcttta tccgtcgctg ggccttgctg
     1741 cgggctgtct tcttgccgtt ctgcttcttt agtctttaca tttcggcggc tttgtggcaa
     1801 actgtcggcg ggtgataata tatgaataga aagatcctct tttttgatgt ggacgggacg
     1861 atcgtgacat cggaccacgt cgttcccgaa agcgcgtgtg ccgcgctgaa aaaagcgcag
     1921 agcgcaggac acatcctcat aatcaacacc ggccgtccgt tccggcacat tgagccgcag
     1981 atccgcgctc tcggctttga cggatacatc tgttccatcg gcggacacat cctgctggat
     2041 gggaaagacc tgctgtaccg aacgatcccg cacacagagg ctgcgcagat ccgtgacgca
     2101 gggtatgcat gcggcatgga tatgctgttt gaatccgagc agggggtatg gatggatgag
     2161 cgatgcacat ccgccatcgc gcggcgtgaa tttgcgtggc tcaagagcat tggagtgccc
     2221 gcctttaccg acactgctgt gccggacttt gcatttgaca agtttgtctg ctggccgcag
     2281 gcgtgcgccg atcccgagcg ttttgagcgc gcgttttccg accggctcga ttttatccgc
     2341 cgcgagcatt ccatgcgcga ggtcattttg aaaggcctat ccaaagcggg cggcatgaaa
     2401 accgtgatgc agtacctcgg ctttgctccc aaggattcct ttgccttcgg cgacggaccg
     2461 aacgatctcc ccatgctgcg cgaggcgggg acgagcgttc tcatgggcaa cgctccgcaa
     2521 tttctttgga aagaggcgga ctatgtgacc gcacctatca cgaaagacgg actcgccctg
     2581 gcgctggagc atttcggact catttgatac ggcagactgc tgtgtagtta tgtaaacctc
     2641 cgggatatag tcccggaggt tttcgcatat acggtcgcgc gagctcatac cttgacagtg
     2701 gacaaccccg caaaggagag cgccctatgg atgtggatct gatatttaaa attgcagccg
     2761 tcggaatcct tgttgctgtg ctgaacattc tgctcacgcg gtcgggcagg gaagatcagg
     2821 cgcttatgac gagcatcacc ggtctcgtgg tcgttctggt gatcgtggtg caggagatca
     2881 gtgagctttt tgatctgatc cgcgatctgt tcgggtttta gggaatgaat ggaaattgtg
     2941 attcgcgccg cggctgcggc ggtgatcggc agcattttgg cgctgctgct tcgcaaatat
     3001 acgccggagc tgtcgctcac ggtgacgatt ctcgcagggg tgatcatcgt atggctcgcg
     3061 gcagcggtgg cttcgcgtat ttccgatctt ctgcgcgaga tcgccgagaa gggcggcatc
     3121 gacgcggtgt atctctcccc ggtgctcaaa tgcatcggga tcgggatgat cacgcatctg
     3181 gcggcgcagg tctgccggga cgcacagcag gggagcattg cctccgctgt ggagctttgc
     3241 ggaacgcttt gcgccttata tatatccctg ccgctcatcc gctcgctcct cgccattgtg
     3301 gagcagctgc tttgatgctg ctcgtactgg cgctcgccgt ctgcccaggg agggcgcttg
     3361 ccgcttcaga tgttccgatc cccgggatgg acgagctgac cgaagctctc ccggaggaag
     3421 cggtgcctat tctttccggc gtttcgccgg aggaactgcc gcaggagagc gtatggcgaa
     3481 cgctcctgcg aacggcatgg gaaaaagtac gttcttccgc cgcggatatc tgccgcacgg
     3541 gagggatact cctttgtgtg tgcgtgcttg tatcgctcac ggatacactt gatctcggca
     3601 gccgggcccc gccgtatatc acatttgccg ccgtcgcggt aatcggtacg gcgacgatct
     3661 cggatctccg ctcgtacctc tcgctgggaa cggagacgct ccagacactc tcggactatt
     3721 cccacgtcct tctgccggtg ctctcgtccg ccgccgcagc gtccggggcg gcggggagcg
     3781 cagcggcaaa gtacgccgca acggcggtat gcatggacgt gcttctgtcc gttgcgcgga
     3841 gcgttctcgt cccgtgcgta tgcgggtatg ccgctctgtc cgttgccgac gccgctgtag
     3901 gaaatgaaat attgaaaacc gcaaagaaga tcatgaaaac ggtctgcacc gctcttttga
     3961 gcgccgtatg cgtggcgttt accgcgtggc tatcgctcac cggcgtcgtt accggtacgg
     4021 cggacgcgct cgccgcgcgg gtgacgaaaa cggcgnnnnn nnnnnggagc attctgtcgg
     4081 atgcggcctc aacgctcgca gccgcggccg ggacgctcaa ggcaacgatc gggatcttcg
     4141 ggctgcttgc ggtggcggcg atctgcctgc cgcccgtttt gacgctcggc gtgcggtttt
     4201 tcgtttataa gctgacggcc gctgtgtgcg agtgtgtggc ggacaagcgg ttttcggagc
     4261 taatatcgaa tcttggcacg gcgtttgcgc tgctgcttgc gataaacggc gcaggggcga
     4321 tgatgctctt tctctccctt tattctctga tacagacggt ggtgtaaacg catgacggaa
     4381 tggctgtttt cgtggatccg ttcgctggtc gcggcgtcgc ttctctccgc gctggctctc
     4441 cagctcacgc cggaggggag cgtaaagcgt gtaacacgct ttacctgcgg cattctcctt
     4501 gcgtgtacgc tcctgtcgcc cgtgctgcgg acggacgaga catcgttctc gctcgcgcta
     4561 aacgactacc ggcggaccgt tgccgagctg acggatgatc tggaggagca ggaaaatcaa
     4621 ctgctgcgaa catacataga acagaagacc gcggcataca tattggacga ggcacgatcc
     4681 ataggaatac cggaaccgtc ggtttcggtg aaggtgaaat ggggggatga gagctgggtg
     4741 ccctgtgaag ttacgatacg cggagagatt accgccgaac agcgataccg cctgagcgct
     4801 tatcttgccg cggagctggg cattccggcg gaaaggcagc actggaccga tgagtgagcg
     4861 gaaatctttg ttaagcgcta tgctgcaaaa gaaaagcgtg ctgatcgttc tgcttgccgc
     4921 ggggctgctg cttctgctct ggcccagatc gtccggcagc ggcacagcag gaaagaacga
     4981 ggatgtgtca actacagagg aaaagcggct ctgtgcggtg ctctcaaaaa tcgacggcgt
     5041 cggagaggtg tacgcgctgc tctccgaaga gccaaaccgc cgaggcgagt ataccggggc
     5101 ggtggtcgtc tgttccggcg caaggtcacc ggatatacgg ctccgcgtca tacaggccgt
     5161 gagcgccttc accggtctcg gcagcaaccg gatcattgtg gaagaattga tatcttagga
     5221 ggatgattat atgaagacgg taaatctgcg tccccatctc aagcgaaaca ttctcattgc
     5281 agcggtgctg ctgctcgtgt gcgcggcggc attcctcaac tggtcgtaca accagcgctg
     5341 gggcgacggc aacagtgcca tggccgacgc ggaggacgaa atgacgactg ccgtaaacgc
     5401 cgcggacggc gcgccggagg aaaccgtatc cgcagcgctc cccgcgtccg tgtccgggta
     5461 tttctcggaa gcgcggctca cacgccagca gtcaagagat caggcgcttt cgctgcttga
     5521 aacggcggcc tgtgccgagt ccgcttcgca ggatgtcatc gacagcgcca tgaacgagat
     5581 caccgttatg gccaactggt ctctgctgga aagcaagatc gaaaacgagc ttcttgccaa
     5641 agattttgcc gactgtgtgg tgtatctctc ctcgcagggc tgcacggtcg cagtcccgtc
     5701 cggtgcggac ggcctgaccg aaacgcaggt cgcccagatc accgatgccg ttctctccaa
     5761 caccgagatg acggccgctg cgatcaatgt gatcgaggtg aggaactgaa aaaagcgcgg
     5821 agggaagatc ccttcgcgct ttttctatgg ggaatgatta cttcatgccg ttttcgtagg
     5881 cctcgatcat tttcttgacc atctggccgc cgatgctgcc ggcctggcgg gaggtaagct
     5941 cgccgttata gccattcgta aggttcacac cgacctggct ggcagcctcc atcttaaagc
     6001 gatccatcgc ttcgcgggcc tgaggattga caagatgatt gctcttgttg gtagacatct
     6061 gattgcaact cctttctctc gaatcgaaca cgtttttgct gtgtccgagc ctattttggc
     6121 cttgacagcc gcggttatgc gaaagaaaga aatgtaatat ttgacagtct caaagctctt
     6181 cggcggataa tatgccgcgg agaatagcat caatgttgct ccgatattcg agaagcgttt
     6241 tctcccatgc ggaaagacag tgccgcccct cgtccgtgat ggagtaaacg cgtctcggct
     6301 tgctggcaga ttcggtgtcc cagtaagatg tgacaagtcc ggccgtctcc atccgtttga
     6361 gcgtgcggta c
//
LOCUS       DAWAAE010000119         6366 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5242_length_6366_cov_2.691966, whole genome shotgun sequence.
ACCESSION   DAWAAE010000119 DAWAAE010000000
VERSION     DAWAAE010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5242_length_6366_cov_2.691966"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1345 a         1911 c         1839 g         1271 t
ORIGIN      
        1 ggacgcatcg atccgcgagc agaccgagac gcgggacgag gagagcgggt tttactcggc
       61 ataagctgcg ggcgctcgaa gccgtcggca tacaggcgga gggcaacgta aaggacgaga
      121 tcaccgaagc aaataaccga ataaagaaat cagcttacag cgatgcaccg ccgtaggctg
      181 atttttttac tgccgctgta aaacaccgca gtcagtgaaa aacaagattg atgagcaaac
      241 acagcagcgc gccgcagagc gtcggcagca cggcggcgag cgccgtccat gtccggctgc
      301 cgatctcttt ttttatggag agaagcgttg tagagcacgg ccagtgcatc agtgtaaaca
      361 caatgacgca cgctgctgtc tttccagtcc agccgttggc gagcaatatt ccgctcatgg
      421 ccgcagcaga aagcgtccgg ttgagcgcac cctcggccat atagatcatc atggcgatgg
      481 ggatcaccgt ttcattggca gggaatccca gaatgaaggc cagcaggatc acgccgtcca
      541 taccgagaaa ttttccgatt ggatcgatca cgtccgacag ataccgcagc agggagacat
      601 ttccgacgga aaaatttccc agcagccaga gaaccagacc ggcgggcgcg gcaacagcgg
      661 cggcccgccc aaggacgaat accgtccggt caaggagcga gcggacaagg atctttccga
      721 gctgtggcct tcggtacggc ggcagctcca gaacataaga ggacgtctcc ccgcacagaa
      781 ccgtggcgga taaaagccgt gttgcctcaa acgtagcggc aatgccgaga catacgacac
      841 ccgtcagcat aagcgcggac acaataggat tacgcgagga aaagaaagcg ccgagcagga
      901 aaatcaaaat cggaaagcgc ccattgcacg ggacgaaact gttcgtgagt atggcaagca
      961 gccgctcgcg cggcgaatcg atgatacggc agccgacaac gccggcggca ttgcagccaa
     1021 agcccatcgc cattgtaagc gcctgtttgc cgcacgcgcg gcagctctcg aacggcttat
     1081 cgagattgaa cgccatgcgc ggcagatagc cgacgtcttc aaggaacgtg aacagcggaa
     1141 agaagatagc catcggcggc agcatgacgg atacgaccca tgcagtcacc cgatatacgc
     1201 caaggatcag cgcgtcgtgc agccagagcg ggattccggc ggcgagaagc cagcgtgaga
     1261 gaatatcgcc aaaggcgaaa agcatggagg aaagaaacgc cgaagggtag tttgcgccct
     1321 gcaccgtcag ataaaagagg atcagcagca acagcagcat gatcggaatg ccgaattttt
     1381 ttccggtcag gatgcggtca gccctccggt cacgggaaaa gccgttttga ttcgccactg
     1441 aaacgacctc cgctgcaata tcggctgcac ggcggagaag caccgttgcc gccgctgccg
     1501 cggtttctcc ggcgcggtcg atgcgctctc tcccgagacg gacagcttcc gaaagaaaag
     1561 gatcctcctc cagcggtctt ccgagctttt tctccagtgc gcaaatcgca gtatcgccgc
     1621 cagtcagaag ctgcatcgct atccaccggt gatttggcat agacccggga agcagctcat
     1681 ccagccgcgc ctcgatcgaa aaaagccccg cctcgatcgc ttcgccatag cgcaccgaaa
     1741 ccggcttgcg gtgtctctcc tgcgcggcgg ccaccgctgc acgcagcagg ggagggagcg
     1801 tttttttctg ccgcacgctc gttccgacaa cgcgcacgcc gagcagctgc gatagccgcg
     1861 gaagatccac ggtgatgccg cgcttttcgg cctcgtccat cagattcaca catataacaa
     1921 cgtgatccgt gatctccatg acctgcaggg caagatagag attccgctcg agcgccgtgg
     1981 cgtcgcagac aacgatcacc gcgtcggcgc tgccgaagga gatatattcc gccgcgacct
     2041 cctcttccgg agaacgggcg gagagagaat acgttcccgg cagatcaatg agctcaacgg
     2101 ccgccccgtc gatgctgcat gttcccgccg ccgtcgcgac cgttttcccc ggccagtttc
     2161 cggtatgctg gtgcaagccg gtcagcgcgt tgaaaagcga gcttttccca acgttcgggt
     2221 tccccaccag cgcaaaacgg tatgtctccg ccattacgtt ttcgcctcat gcggcaggcg
     2281 gacgagcacg gaggcgctgt ccccggcgcg gagggcgatc accgttccgc aaatttcgta
     2341 ggccgtcgga tcaccgaaca tgctgcggaa gaggcatcgt attcccgcgc cctccgcgaa
     2401 cccgagatcg agaaggcggc ggcttaaggc acactccggc aatatatccg tgataattcc
     2461 ctgcgtaccg ggctccatat ctctcagaga aatgattgat tccatggcaa attccgccct
     2521 ttccggtaat gtctttcctc tgtattctat tcgcccgtac tcgtccggtt ccttgacttt
     2581 ttgcgcggaa gagtctacca taaaataaaa aacattgagg atatgaccat ggttgaagtc
     2641 gttgcagcgc ttgtccacga tacggaaaat ccgcggcgct ttctcatctg tcaacgcccg
     2701 gcgcataagg cgagaggact gctctgggaa tttgtcggcg gcaaggtcga gcccggggaa
     2761 acgaagaggg aagccctcgc gcgcgaatgc cgggaggagc ttggcgtcgc agtctcggtc
     2821 ggtgatgtct atatggagct gacgcatgtt tatcccgatc tcaccatcca tttaacgctg
     2881 ttccatgcgg agatcacgga cggtgtgccg cagcttctgg agcactgcgc catggactgg
     2941 atcacgccgg aggagatcgg cggctacgat ttctgcccgg cggacgaaga gatcctcgcc
     3001 cggctccggc ttgcgggaaa aacggcagcg gaatgattct tccgccgccg ttttctctct
     3061 gcttcagcgt ccggggaacg ccggattcat atagtagacg ttgttctggt tgagcttttc
     3121 cgtcacgaga cccagcgcct caccgaagcg ctggaaatgg acgacctccc gctcacgcag
     3181 gaaacgaatc acgttattta cgtcgggatc gtccgagagc cggagaatgt tatcgtaggt
     3241 ggcgcgcgcc ttctgctcgg cggcaaggtc ctcggtgaga tcggcgatca catcgccgct
     3301 gacgccgatg ctgatggcgt tccacggcac gcccgccgcg gcctgcggaa atacgccgag
     3361 cgtatgatcg acgaagtaag cgtcaaagcc ctgcgccttg atctcctccg gcgtcagatt
     3421 ccgcgtgagc tggtggacga tcgagccgac catttccaga tgcccgagtt cttccgtgcc
     3481 gatgtcggta aggatcccct tgacttcatc gctcggcatg gcaaagcgct gactcagata
     3541 gcggagcgac gcccccagtt cgccgttcgg tccgccgtac tgggagatga tgaccttggc
     3601 aagcgccggg ttcggattgg caattcggac aggatactga agattcttct tataaataaa
     3661 catacgtcaa ccctccgtgc cgccgcggta ttcccacggc cagggacttt tcagccaggt
     3721 atacgtttcc gcgttgagaa gatcgcgttt ggtgatcggc ccatagagct ttgcatagcg
     3781 ctgcgcgcct tcctcggcga gccggagcag atcctgatag gcagcaaacg cctccgcgtc
     3841 gccggcgtgc gtgtcgagat acagggaaag atcgtgcgcg gcaaaatcga tcgccatgag
     3901 ctcggcgagc ggaacgttcg gcgcggcgtc gttgaccatg ttcccgaacg gcagatcgag
     3961 ccccgggaaa agcgtgccgc gtgcaagcgc tttgtccggc tcatactgcg cctgcgcgtt
     4021 ttcctgcgca gggacgaatg cagctgccag cggcgcacag gacggcaaag cgccgcattt
     4081 atatccgcag acggcctccg cggatccctt actattcaaa acgattcctc caaaaaaccg
     4141 gatagagagc tcagctctca gcccatcgta tgccgaaagg aaacgccgtg accgtttttt
     4201 tgaaaaacat aaaatcgcgg ttgctttttg gcatgtaaat gttatactat tatgtaaagc
     4261 ggaatattac ggaaccatgg aatggggtta acgatatgaa agagattttc aagaagaaaa
     4321 cggaagagag cggcgccggt tctgcgcctc tcggcgggac aacatggtac ggtgtggaca
     4381 agctgttgtt tgtccggatc ggcattgccg ccgttttgct ctgtctcggt ctctttctgc
     4441 agctgggaga cggcataacg cttctcctgc tgctgctcag cacgctcatc agcggttttg
     4501 acgtgctgta tcaggcctgc gtccggctcg tccgggaaca ccgtcttggg gaggagctgc
     4561 tcgtttccat tgcagcgatc ctcgccttta cgatcaacga gggctatgag gctgccgccg
     4621 tcatgctgat ctatcaggcc gggtgtatcg tccgcgcgta cgcctccgct cttacgaagt
     4681 ccagcttccg cgaccgtgtg gacccctatg tggcgagtgt caccgttctc cgcggtgagg
     4741 aaaagacctc cattccgccg gagaatgtcc agcttggcga tatccttatt ctcgaacgcg
     4801 gccagcgcgc tccggccgat ctcgaaattc tggaaggctc cgtcacgctc gatctcgccc
     4861 cgatcttcgg acataccgcc cgccgcgaag tcacggccgg acaaaagatc cctgcgggcg
     4921 ctgtcaacag cagcgctgcc gtgcgctgca cggtgctcgc ccccgcctcc gattccgttt
     4981 tcgcccgcga gatcgacacc gtttccgatc cggaggctgt ctatggcccc gaggccgatt
     5041 cggtggaacg ctatgcgaga gtttacgccc cgttcgctct cggtgtgagc gtcctgatcg
     5101 cgcttctcct tctgatcttc tccacggtcc cgaccgagga agccattcac cgcgcgctcg
     5161 ttctcctcat catttcctgc ccgacggctt tccttgcgcc gctgccattt atgtaccttg
     5221 ccggactctt ccgttcgctg caaagcggcg ttgtcgtgaa ggacgccttc gttctggacg
     5281 gcctttcccg cgcaggcgcg gttgtgttcg ataaggacga aatgctctcc accggctttt
     5341 accgtgtggc ctccgtgaag tcggagcggc tcgaccccgc ggtgctgctc aaggttgccg
     5401 cgcacgccga atccggctct tccaccgccg tcgccgcatc catcgccaag gcgtacggac
     5461 aggcgatcga cgcctctctc attgaagaat tcaccgaaga gcccgacggt atcaccgccg
     5521 tgatcgacgg gatcgacatc cgcatgggcg atgcggccta tatggagaaa aacggcgtca
     5581 ctgttaccga ccctgtcggg gacagcatta ccgtgtatat ggccgtcaac gaccagtatg
     5641 ccggctgcat ctatctctcc gatacgatcc gcgacgatgc cagagcctcc ttcagtgccg
     5701 tgtccggcgc gggatgcgag tgcatcatgc tctcggcgga cagccccgaa aagacccgta
     5761 ccgctgccgt ttccgccggt gtgcgcgagt attacgcgca atgcatgccg atggaccgtc
     5821 tggaaaaaat ccaggagatc aaggaacgct tcccggtcaa cagcgttttg tacatgggcg
     5881 aggcaaacgg cgataccgcg tgcttcaatg cggcggatat cggcgtgtgc atcgacggtc
     5941 tcgcgtccga ggcctccatg cagacgggca gcgccgttat catggacaag acggctgcgc
     6001 ccatcgccgg cgcgatcgac gcggcaaagg ccacgcgctc cacagtacgg cagctgatcc
     6061 ttgccgttct cgccgtcaag gccgtgctcc tcgttctggc gctcctcggc gttacctatc
     6121 agctttggtt cgcggccatg gtcgatacgg ttgccggtat tgcgggcatt ctctattcct
     6181 cccgcatctg gtctgagcag aaataaattt tccataaact tttgaaacgg gttggaattg
     6241 ctccaacccg tttcagcctg tcaaaaaagc ctcgcagagt ttgcgcccgc aggcgcaaat
     6301 aaagtgaaaa acatttttgc cggcggcgtg tacgtcggca aaaatacttt tcggctcgca
     6361 aacgtg
//
LOCUS       DAWAAE010000120         6262 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5349_length_6262_cov_3.473014, whole genome shotgun sequence.
ACCESSION   DAWAAE010000120 DAWAAE010000000
VERSION     DAWAAE010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5349_length_6262_cov_3.473014"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1276 a         1928 c         1758 g         1300 t
ORIGIN      
        1 accgtctcga caagaccaac atcatccact gccccatcgg caaggcttcc ttcggtgcag
       61 agaagctgct tgagaactac accgctctca tcaccgccat cctgcgcgcg aagcccgcga
      121 ccgcgaaggg ccagtatatc cgcagctgcg tgaccgccac caccatgggc cccggcatca
      181 agatcaacgc ccagaagcag ctctgatttc ggatcacaaa aaatcgtcga atgaaaattc
      241 ggcgattttt ttattcacaa aatgttcaag atagtttcag gttcttttca gaattccgtg
      301 ataggataca gccaagaatt gaaacaagag gtgttttcca tgtacgagaa caacgatccc
      361 aataccgaaa ataacaaaag cagcacgtac cggtacacgc cggagcgcga cacgcagagc
      421 gattataccg cgggggcgca taacaccgat tcacagtact cttccggtcc gcagagcggc
      481 tacattcccg gcagctattc gtattccccc gagccgccga agaaaaagaa gaaactctcc
      541 gccggaaaga tcatcgccat cgccctcgcg tgcgcgctgc tgggcggatg cctcggcgtc
      601 ggcgcaacgc ttctgctgcg caataacacg gaaaaccaga ctccctcgac cggcacgact
      661 atgtacgtcg gcgagcggca gaccgaaaag gtgaataccg cgcgtaccga tacctccgag
      721 aagctcacgc cgcgcgaggt atatgcgcag aacgtgaatt ccaccgtcgg catcacgacc
      781 tcgatcacga cgaactactg gggctatcag acaacgagtg ccgcttccgg ctccggcttc
      841 attctgaccg aggacggcta cattctcaca aactaccacg tgatcgaaaa ctccagcagc
      901 gtgcaggtaa cgacttacga caacacctcc tacagcgcct cgatcgtcgg ctatgacgag
      961 agcaacgaca ttgccgttct caagatcgac gctaccggtc tcacgccggt cgtgcttggc
     1021 agctccgacg atctttatgt cggcgacgag gtcatggcga tcggcaaccc gctcggcgag
     1081 ctgacgttct cgctcaccgt cggctatgtg agcgcgcttg accgcgaggt cacgctctcc
     1141 tccggcacaa cgatggatct catccagacc gacgccgcca tcaattccgg caactccggc
     1201 ggcgcgctct tcaacagcta cggcgaggtc gtcggcatca cgaacgccaa gtacagctcc
     1261 tcctcttctt cgggcagcgc gtccatcgac aacatcggct ttgccattcc gatcaaccac
     1321 gtgaagaaca tcatcacgag catcattgaa aagggctata tcgtcaagcc gtatatcggc
     1381 gtttccgtaa cgaccgtgag cagcgaaatg gtcagctacg gtctcccgca gggcgcggcg
     1441 atcaaggccg tcagtgagaa cagccccgct gcggaatgcg gactgaagat caacgacatc
     1501 atcaccgcca taaacggcga ggagatcaca acgagcagcg agcttgtgaa gatcgtctcc
     1561 gccagtcagc ccggcgacga gatgacgctc accgtctacc ggcagggtga gagcgagcag
     1621 cttacgctca cgctcaccgt cggcgagcag cagaagagcg ctctggccga tcccacgtcg
     1681 tcctcgaacc agcagcagtc ctccggttcc tacggcggtt tcccgttcgg cttcggctat
     1741 ggttacggcg gctaaatcat cgcccctatt gaaaaaagaa aaggctctgt ttctccggaa
     1801 acagagcctg agcttgtcaa aaaagaattt ttgacaagct ctcaaacgag acccacttcg
     1861 ttgggctctc gtttgaaaag gctgcggctc gccgcagcgc actcgcttcg ctcgcacgtt
     1921 tgcgagccga aaagtatttt tgccgacgta cacgccgccg gcaaaaatga tttgaacttt
     1981 atttgcgcct gcgggcgcaa actctgcgag gcttcttcga caggttgtgg ctctgtttct
     2041 ccggaaacag agccttttct tcttatttgc ggagcaccga ttttcccatg tacttccagg
     2101 tgtccttgtg gccgccgtaa cgcatcgcct tccggcagcc ggggatgtcc cagcagagag
     2161 tgccgaaaag cgccgtcacg ccgtgatcaa accagatcgg cgataccgga acgctcggcc
     2221 cgctcaacgc tacgatctgc gagtcgcggc aaagctcaag aatatgcggc atcgtgccgt
     2281 tcgtgagcgc catgccggtg atgacgacga catcggagcc ggggagcagc cggtcttcct
     2341 cccgttccgg atagtcgccg gggcgcgggt ccttttcaaa cacacgcagc tcccgcatgc
     2401 ccgcgagctt ttcgccgccg cgaaagtggc ctatcgtaga aacgagcttc ccggcgcact
     2461 ccggttccag tgtgcggaac acgtcgccct cgtcgatctc cggcggatag agcgtaacgc
     2521 cgagggcctc catgctctcg cgccggttgc accacgcgtt caccgccgcg cagccgagcg
     2581 ccgagagcgc catatcgtcc gagccgagct gcgctgcaag atcgcgcagc ggctttccca
     2641 gccagtccgt acagacggcc gcgtcgccat acggtttttt ccccggcagc ggcagcgagg
     2701 caagccccgc gccgccctcc gtttccacat atgcctgata ccgcccgcgc accgccatgg
     2761 taacgcgcag atgcgccggt acggaagaaa taaacgcttc ataaagttcc atacggattg
     2821 taccttgcct ttctgttttc gccggagtat aattagtttc agtatacagg aatttaccgc
     2881 atatcacaag tcaggaggaa tacccatgcg aaggattttt tccaagctgc tttatgaaat
     2941 cgaagaagag cgcaatacta tgctcgtaac agtcatcgcc gatcgcggca gcgcgccgcg
     3001 cggcgcgggc gcggcgatgc tcgtcggcga aaagggccgc atcaccggca cgatcggcgg
     3061 cggcgccgtg gaatatcagt cggagaaaaa ggcgatggag cttctcaaag ccggtgagag
     3121 cgccgtgcat gagtttgtgc tccggcccaa cgataaagag gacatcggca tgcagtgcgg
     3181 cggcgatgtt acggtgctct tcgagtatgt gagcgccgac gatctccgct ggagcctgct
     3241 tgcaggcgcc gtgtgcgacc gtatcgaagc gggcaagcac ggctggctcg tcctcccgct
     3301 cgacggcagt gtgccgagcc tgtacggtga ggaaggcctt ctctgcggcg agggcgtaag
     3361 cgaggaagag cgcgccgcgc tctgcacgac gcatacggtc gtcacaagca cctattacgc
     3421 cgaaccgctg ccgctcttca accgtgttgt gatcttcggc ggcggccaca tcacgcaggc
     3481 gctcacaccc atgctcggca cggtggattt ccgctgcgtc gtgctcgata accgtccggc
     3541 ttttgccgac atttccctct tcaagggagc tgaggacgct atagtctgcg attatgataa
     3601 cattgccgag tccgtaacgc tcaccgccga ggactatgtt gtcgtgatga cgaacggcca
     3661 ttccggcgat ctcattatcg aggagcaggt gctccgctcg ccgcacgcct acctcggcgt
     3721 cgtcggctcg cgcagcaaga tcgcgttcgt caacaaaacg ctcatggacc gcggcattcc
     3781 ggaagaggcg ctaaaggccg tgcatacgcc gatcggcacg cccatccgcg ccgtcacccc
     3841 ggcggagatc gccgtgagca ttctcggcga gctgatcctc tgccgcgccg agatccgcga
     3901 gggcgaaatg cccgcgcatc acggctgccc gatgcacgga taagcagcat atatcccata
     3961 agctcatgcg tacataagat catcccccgc ggacatcgtc tgcgggggat gatccatttg
     4021 tcttttcaaa ccgctccccc agcgaagcgg gggttgtttg aaggctttag cccagcatgg
     4081 ccgcggcttc ttccgcggtc aggtcgtagt tccagaacac cttatagatc tcctgtgcct
     4141 tggcggtcat gtcgtagtca tagacctccg ggtacagcag attgccgagc caccacacac
     4201 cgaggatcat gttcatggac gggggcgcgg acagccagca gtagagatcg cacggcacgg
     4261 tgtagacctt gccgtccttc acggcgcgca gctcctgcca cgcggcctcg gtctcaatgg
     4321 tctccagagc cacagcgtcg gtcacgagga caacatcggg atcaaagttg tagagctgct
     4381 ccatgttgat catgttgccg ccgcccttat tgctgacatc ttcgagcaca acggcgttct
     4441 tcgcgccgac aataccaagc gcctgtgcct gagaagagcc ctcggcgttc gtgccgagag
     4501 gatcgctgcc ggagctgtag agcacggaga gctgttgatc ctccgtgatc ttcgcgctgt
     4561 tttcggcggc catggtcatg gtctcctcaa cgagcttggc aagctcttcg ccgcgctctt
     4621 ccttgccgag aatggtgccg agcgtgcgga aggcgtccgc caaatgggca agatcggctt
     4681 caatgaagac gcagggcaga ccggtctgct cctgaagcgc cgtcagatcg tccgcaatgg
     4741 actttttgaa atcgccgagg tcgatgatga cctccgcgcc ggtggcgaga agctcttcaa
     4801 gattcagcgt ggccttgccg ccgtacagct ggccggtcac aggcagatcg gcgatgccgt
     4861 cataaagata cgggatctga ttttcgctca ccttggtgcc gacgcagacg agctcttcgg
     4921 gcgcgaacgc cgcaaggatc atcgtggcga ccgtgccgct gggagcgatc ttcgtaatgt
     4981 tggcgggcag ctcaacttcg cgtccgcacg aatcggtgaa catgaccgtt tcggcagcgg
     5041 gctcttcggc gggatcttcc gccggggctt ccgttgcagc aggctcttcc gtcaccgcgg
     5101 gtgcgggctc cgcgggagtc ggttccgccg cgccgcacgc ggcaaggctg aacagcatca
     5161 ccagtgcaag aagcagggca agtgtttttt tcatggatgt tctcctctca ttatattctc
     5221 ttgcgagaat attttcgtgt ggagtaaata taacagattt cgtgtaattc tgtcaattgc
     5281 tttttcgtcg tcgctatgct acattacata tgacatctgt cggcaaaaaa cggaggttaa
     5341 tttatgcgtc gttttactcg tctcaccggc gctctgctcg cactgctgct cgtcttttcc
     5401 gttctttccg gctgcggcgg cgctgcgtcg ggcgtgccgt cctgcactgc cgcccccgct
     5461 gcggacgctg tccccgcggc agacgccccg tcgtataccg aagcggacgc cgtttccccg
     5521 cgcgagtcgg acaagtcctc cccggctgcg ctcgatgagg gcggggagta tacctcggcg
     5581 gaggacgtgg cgctctatct gcatctctac gctcatctgc cgcagaactt catcaccaag
     5641 aaagatgccc gcgcgctcgg ctggaacggc ggcgggctgg acgattacgc cgacggcaaa
     5701 tgcatcggcg gcgaccggtt cggaaactac gaaggacttc tgccggacgc gcccgggcgg
     5761 gagtaccatg agtgcgatat cgacacactg cacgccgcct cgcgcggggc gaagcgcatc
     5821 gtatactcca atgacgggct tatctattat accgaggatc actacgagag ctttatattg
     5881 ctttatggcg aagaatgatg ttcttttgag ccattctttc tatattttat ctatttcatt
     5941 tccgctttcg tgtgctataa tacaatttgg ttttaaaatg tcttcttatg taatgtgagg
     6001 aaaatacatg gatcaaccta ttgtgcaggg gctgctcacc tttctcggcg gtgccgtcgt
     6061 tgccggtctc aacgccctgc tctccgcccg gctgctggac aaaaagcctg cagctttcgc
     6121 ttcgttctcc gcagtacggc agctgttcaa tgttgcctat cttgttctgg ttttctttgg
     6181 ttcgcgcgct ctcggctggg acgttacggc gccgctcgtc ggcgcggcgc tcggtctgac
     6241 cgcgctttcc atggtttttg cc
//
LOCUS       DAWAAE010000121         6254 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5356_length_6254_cov_3.609614, whole genome shotgun sequence.
ACCESSION   DAWAAE010000121 DAWAAE010000000
VERSION     DAWAAE010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_6254_cov_3.609614"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1734..1743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1240 a         1944 c         1779 g         1281 t
ORIGIN      
        1 ccgggcggcc cgccagcggc gctcgccggc gatgatctga tagtaaccct tgtcgagcgg
       61 acgcaccgtg atgggctgga tcagcccgta ttcgcggatg gacgccgcca gctcctcgat
      121 cgccgcgggg tcaaactccc ggcgcggctg gtccttgcgc ggctcgaccc gcgagatcgg
      181 caggatctgc accgtttcgt tttcgaggac gttttcctcc tcgccgaaca gggcggaaag
      241 cccggtcccg agccgtgatg tcttctcttt tgccatcttc atccctgcct ctgcaaaaat
      301 tcctcggcgg cagcgagata tgcgcgcgcg cccttcgatc cggcgtcgta ttccgtgatc
      361 gggcggccgt ggctcggcgc ctcggccaga cggacgttgc gcgggatgac cgtatcgtac
      421 acccgcgcgc cgaaataccg ccgcagctct tcggccacct gcgtggaaaa gttcaggcgg
      481 ttatcgtaca tcgtgagcag catgccctca atgtagagat tcttgttcag ccgccggttg
      541 gcgatcttca ccgacgtcgt gagatcagcc acgccctcca tcgcgtaata ctcgcactgc
      601 agcggcacga gcaggctgtc cgccgcggtg aatgcgttga gcgtcagaat gcccagcgac
      661 ggcgggcagt cgatgaatat gtagtcaaaa tcgtcccgga tcgtgtcgat cgcttcgcgc
      721 aggcggtatt cgcgccgctc tacgttggcg agctcgacct ccgcgcccga aagatcggtg
      781 ttcgacggca gcacccagcc gtacggcaga cggatgacgg cgtcctccgc gctcgcgctg
      841 cccatcacga cgtcgtacgc cgtcggattg ctccgcttgg acacgcccat accggacgta
      901 ctgttgccct gcgggtcgaa atccacaagc agcgttttct ttccgagtct cgccagcgcc
      961 gctgtaagat tcacgcttgt tgtcgttttg ccgacgccgc ccttctggtt ggcgacggcg
     1021 atcactttgc ccatctcttt gccccctttg ggtctctcaa cgtccggcgc ggacggagca
     1081 gcgccccgcc tctgcgtgcg cggccgcttc gccgccgaac tttcgtttct gttttatagc
     1141 accgtttatt ataatgatgc gctttccatt ttgcaactgt ttcacgtgaa acattttcag
     1201 cggccgaacg cttcgggaga tgtttcacgt gaaacactgg tttttcgtca aaaaaggcgc
     1261 ggcctgatgt ttcacgtgaa acatcaggcg aggaacatat caatgtcctc gatggtgaga
     1321 gcgtcgtgca gtgcgagatc gatcgcgtag ccgacaagcc gggcgctgtc gcgcacgcgg
     1381 gtgtcgatat cccgcggcgt gacgaaaagc tccgtatcct ccggaagatc aatctccgcg
     1441 ccggagaggt cggcgcagag cgtgcgcgca tccacgaccg tcggcacccc gacagcgacg
     1501 accggaacgc cgagcgtctc ctccgtgaga gcgctgcgcg cgttgccgac gccggagccg
     1561 gggacgatgc ccgcatccgt tacctgcacc gcgcggcaga gccgcgccgc atcgcgcgcc
     1621 gaaagcgcgt ctacggcgat cacacgatcc ggccggacaa gcgatacaac gccgcggacg
     1681 agcgccgcgc tttctatgcc gcccgccgat gatcgttttc cctgatttca tgcnnnnnnn
     1741 nnnaactctt ccgggagccg tgttttcaaa tggcgcgtga cgagaacgca ctcggcggcg
     1801 agcggcccga cggcgtccgg ggtaatatcc ggattcccca gaccgacaac aagaacgctt
     1861 tcctccggct ccatggcaag ctgcgtgcgg atctcgcgcg cgagaacgcc gcaggcgcgg
     1921 cggaaggcgt cctcctcgcg ccggacgagc gcgtcgagcg agatcgtgac atagcgcccc
     1981 atcggtttac ataactcttc ggcagctccc tcatccagta tctccacggt ctcaacgccg
     2041 agaccgtcgc gtgtctcctg ccgcgccgag acgcccgcga gagcggcgcc gcgcgttcgg
     2101 agccatacgt cgcgcgactc gcacgcgaga tcggttcgga aagaattttt caaaagattt
     2161 tcctcctgtg ccatatcttg tggtcagtat ttgaaggaga cacaaaagta tgcaaaattt
     2221 ttcgggagaa aacgaaaaaa gtgcttgcaa attcaggaat cctcctttat aatatcaaaa
     2281 cgcgggttcg tcaatccgca ggagtattga atggaggagg tggctaaaga atggccaaca
     2341 tcaagtcttc ggccaagagg gttctcgtct cccgtgttcg cgccgagcgc aacaaggcag
     2401 acaagtctga gctcaagacc acgatcaaaa agtttgacgc cgctgcgacg gaaggaaata
     2461 aggagaacgc cgaggctgcc tacaaagccg ctgtcaagac ggttgaccgc gcggtatcca
     2521 agggtctgct ccacaagaac aacgcagctc gcaagaagtc cagcatggct tccaaaatga
     2581 acgcgctgag cgaataatcc tcaaaaaccg gacagtgcgg cagatatctg ccgcactgtt
     2641 ttttattata aagattttgc aaatttgcaa aaaagacata aattttaaga gaagatacat
     2701 tataataact atgcaggcag ggcagcgccc ctgcataaac gaaacgacaa cttttgcgag
     2761 gtatctacta tggccaagtc cctaatgatg ctggtgaacc ccgccgccgg gcggtcaatg
     2821 tctgatctca atctcggcac ggcagtctcg acgttcgctc tgaacgggta ttgcccgacc
     2881 gtcttttata cgaccgcgcc gggcacggcg tcgaagatcg ttgaagagaa cgccgcgaac
     2941 tacgagcggc tcgtatgtct cggcggcgac ggcacgctct ccgacacgat cgcgggcctg
     3001 atgacgctgc cgcaggagaa gcgccccaag ctcgggtaca tcccgatggg cacggcgaac
     3061 gacgtggcaa acacgctcgg cctgccgatg cgccagccgg ggcgcgccgc ggaaataatc
     3121 ctcgccggaa cgccgctgca ctacgatgtc ggtgaaatgg acagcgtagg cttctttacc
     3181 tatatcgccg cattcggcgc gttcaccgag gtgagctaca agaccgacca ggagctcaaa
     3241 cgtacgctcg gccatctggc gtatgtgctc ggcgggctga acgccctttc caccatcaaa
     3301 agctacaagg ccgtcattga gcacgaaaac ggcgtcgtcg aaggtgaatt catttacggc
     3361 gcgatgtcca actccctgag cgtcgcgggc atggtgcgcc tgaacgacga tctcgtcgat
     3421 ctctcggacg gcaagtttga gattttgctc gtccgccgcc cgaaggacgt gatggaagtc
     3481 aacagcatcc tcacgggcgt gctgaacaag acttacgaca gcccgtgcct caccgtagcg
     3541 cagtcgagct tcgtgaaagt cacgtttgag gagcccgtgc cctggacgcg cgacggcgag
     3601 gacggcggcg cacaccggac gcttactctg cgctcccacc gccacgccgt ggatatctac
     3661 tgctgaaaaa caaaatatag tagttcgccg cctctcttcc ggcggcgaac atccatatat
     3721 gcccccgtgc cgtatccttt ccggagaccg gcgcgggggc atttcgccgt tctgcacagt
     3781 ttctggattt actctgctaa attttttgca taataatttc ttgcaaaatt caaatttgtt
     3841 cttgcaatcg ccgttttcag cgtttataat gcgttcatac gaaagttatt tcctgcggct
     3901 tgcaaatttc gcggttttgc gggaagtgca tccgtcagaa tgaaaaggag gaaatgaaaa
     3961 tgaaaaaaat tctggctctg gtattggctc tgtgcatggt gttcgcgctg tgcgcctgcg
     4021 ggagcagctc gggtgattcc gacaccccgg cggcaagcac cgtttcgctg aacgtcggca
     4081 ccggcggtga gagcggcacc tattacggct acaccggcat cctcggcacc gtgatcggac
     4141 agaagaccga tgttaagctc acggtcgttg cgaccggcgg ctccaaggat aaccttgtga
     4201 gcatgcagga cggcatgtac gatcttgcaa ccgtccagtc cgacgttatg acctacgcct
     4261 acaacggcac gaactccttt gccgactccg gcaagatcga cggcttccgc gttctgtgcg
     4321 ctctgtatcc cgaaacggtg cagatcgtca cgaccgatcc cacgctcaag accgtggaag
     4381 acctgcgcgg caagcgcgtg tgcgtcggcg acgtcggcag cggcacgtac ttcaacgcca
     4441 tcgatgtgtt cgccgcctac gacatgacca tcgacgatgt ttcccccgta ttccagtcct
     4501 tcggcgacag caccgagagc ctcaaggacg gcaagatcga cgccgcgttc ctctgcgccg
     4561 gcgctccgac cccggccgtt accgagctcg ccgcctccaa aaccgtgtac atgatcggct
     4621 ttgacgacgc gcacatggaa aagctgctcg cttcctgccc gtggtacgcc aagctcgttc
     4681 tgcccgccgg tacctacgcc gggtttgacg aggactgcgc caccatcacc gtgaaggcta
     4741 cgctcgtctg ccgcgccgat cttgacgacg acaccgctta caccatcgtc aagaccatca
     4801 tggagagcaa ggacgacatc gccgccctgc acgagaaagg caacagcatc gatctgacct
     4861 tcgccaccga gggcatcgcc gttccgttcg ctgcgggcgc cgcgaaatac tttgccgaaa
     4921 acggcatcac cgtcgagacc gccggctgat cctcccggct gtctcctctc accgggggcg
     4981 caaggccccc ccgggcggag cagatcccgc ccctacagga taacctgaat ttcccgtagg
     5041 ggaggggctc tgctcctccc ctttgcgtat gtaaaagatt ctttttttcg ggaggtagcc
     5101 ccatgtctca cgacgcgaca aaaaatctca acgatgtcat gcgcaagtat gaccgcgaat
     5161 cgaatacccg tgcctgggag ggcaagccga agatcgctgt gcaggcgtat ctcgcggcat
     5221 tcgccatttt cgaggtgtat gtcacgctgt ttggcacgat gctcgacgag gtgcggctca
     5281 cgtcgttcgt cggcgggctg ctgatcgcag gctttctcat tttccccagc cacaagcacg
     5341 agttccgcgt gaactatgtc ccctggtacg actgggtcat catggcgctc ggcgcggtat
     5401 cgttcttcta tttctgcttc aacgccaaca ccatcgtgaa gcagctcccg gcgctcaaga
     5461 cgtacgaggt cgtgctcggc gtgatcggca ttctcgcgct ctttgagctc tgccgccgga
     5521 gcaccggcgt gcccatcatg atcgtggcgg gcgtcttcat tgcgtacgcg ctctactact
     5581 acggcgtcaa gcgcggactc ggcgcggggg cgttcaaatc gctcgtctgc tcgctcttct
     5641 ataaaaagac gggcattctc tccacgccga tcaacgtctg ctccaagtat atcgccgtat
     5701 tcatcatctt cggcgcgttt cttgagcgca ccggcatttc cgatttcttc atccggctcg
     5761 caaactcgct ggccggctcg tccgtcggcg gcccggcgaa ggtcgccgtt atttcgagcg
     5821 cgctgtgcgg catggtgtcc ggttcgtccg tcggaaacac cgtaacgacc ggcagcgtca
     5881 cgatcccgat gatgaaaaag accgggtata aaggcgaatt tgccggtgcg gtcgaagcgg
     5941 cggcatctac cggcgggcag atcatgcccc cgatcatggg cgcggcggcg ttcctgatgg
     6001 tagactacgt aagtctccca tattcaaaca tcgtcgtccg ggcgatcctc ccggcagtgc
     6061 tgtattttac cggcattttc atctgcgtgc atctcgaggc gaagaaatcc gggctgcagg
     6121 gtgtgccccg ccgcgagctg cctcccgtga tgccgcttct gaaaaagacg tatctgctgc
     6181 tgccgctcgt gatccttgtg tatctcgtct cgtccggcag ccggacaatg gcctttgccg
     6241 ccgcggtagc aacc
//
LOCUS       DAWAAE010000122         6185 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5415_length_6185_cov_3.289070, whole genome shotgun sequence.
ACCESSION   DAWAAE010000122 DAWAAE010000000
VERSION     DAWAAE010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_6185_cov_3.289070"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1236 a         1935 c         1961 g         1053 t
ORIGIN      
        1 gaacggcgcg ggcaagacca ccgtttttaa cctccttacc aaggtctatc agccgacccg
       61 cggcaccatt ctgcttgacg gcatggacac ctccggcaaa acgaccgagc agatcaaccg
      121 catgggcatt gcccgcacgt tccagaacat ccgccttttc agtaacctca cggtcgagga
      181 caacgtgaag atcggcctgc acaatcagga gccgtattcc acgtttgagg gcatattccg
      241 cctgccgcgc tactggcggc aggagcgcac cgcgcacgag cgcgcgctgg agctgctgtc
      301 cattttcgat atgcaggaca tggcaaacgt acaggccggc tcgctgccct acggcgcgca
      361 gcgccgtctg gagatcgtcc gcgctctggc aacgaacccg tcgctgctgc tgctcgacga
      421 gcccgccgcc ggcatgaacc cctccgagac gcaggagctc atggagaaca tcgtcaagat
      481 ccgcgacatg ttccagatcg ccgtcatgct catcgagcac gacatgaagc tcgttatggg
      541 catctgcgag ggcatctgcg tactgaactt cggcaaggtg atcgccaagg gcaccgcgga
      601 ggagatccag gcgaaccccg ccgtgatcga agcctatctc ggcaagaaga aggagggctg
      661 acgcatgctt ctcgaagtca aggacattca agtgtattac ggcgcgatcc acgccatcaa
      721 gggcgtctcc ttcgaggtca acgagggcga gatcgtaacg ctcatcggcg cgaacggcgc
      781 gggcaagacg acgacgctcg acaccatcgc gggacttctc cgcagccgcg gcggcgacat
      841 cgttttccag ggcgagagca ttgcgcacaa ggcccctcac ctgatcgtca aggacggtct
      901 ggcgctcgtc ccggagggac ggcgtgtgtt cgcgcagatg accgtggagg aaaaccttga
      961 aatgggcgcg ttcacccgcg ccaactccac cgtcgagccg gggctcgaaa aggtgtacga
     1021 gctgttcccg cggctgaagg aacgccgccg tcaggtcgcc ggaacgctct ccggcggcga
     1081 acagcagatg ctcgccatgg gccgcgcgct gatgagcgcg ccgaagctca tgatgctcga
     1141 cgagccatcc atgggtcttg cgcccatcct tgtggagcag gtgttcgata tcatccagtc
     1201 gctgcacaag gcgggaacga cgatcctgct cgttgagcag aacgcgcaga tggcgctctc
     1261 cattgccgac cgcgcgtacg tgctcgaaaa cggcaaggta tccctctccg gcaccggcga
     1321 ggagctcgca aagagcgagc aggtgcagaa ggcctacctc ggcggataac cgctgtcata
     1381 aaagttgccc cccagtttta ctggggggtt attttttcgc gccggcaaaa actggcggtt
     1441 gacggagagg gggcgggatg gtaggataca gttacaaatt gtaacatttt aaatcacgac
     1501 cgagaggagg gaaggccttg ccgataaatt gccgctttct gcgccgcgcc gcggcgctta
     1561 tattggccgt gctgctgctc gccgcggcga tgccgtccgc gctggccgcg gatacgagga
     1621 ccggcacggt gagtgccccg acgggtatcc tccttctgct cacatcgccc ggcggcgaga
     1681 ccgcggggga ggtgccggac gggagcgccg tttcgatcct tgccgaggag acggacgcga
     1741 acggcacgct ctggtattac atacaaaagc ccggcggcga gaccggttac gtcccggcgg
     1801 agagcgtgac gctctcagag ccggagacga ccccgacgcc ggaggaaacg ccggtatccg
     1861 aaccggaacc caccgcggcg gatgaccgcg atgcctatct tacgcagctc cgcgcgctcg
     1921 gcttcccgga gagctatttg gaaggcctct ggcagctcca cagccggtac ccggcgtggg
     1981 agttccggcc gttcttcacg aatgtcgatt ggaacaccgc cgtgaacgag gaaaacgtcc
     2041 tcggcaaaag ccttgtgtgg ggcagcgccc cgtcctcgtg gaaatccacg caggagggcg
     2101 cgttcaactg gacggacaac acatggatcg agctggacag cggcggctgg gtcgccgcgt
     2161 cgcgcgagat catcgcgcac tatatggacc cgcgcaattt tcttgacagc ggcgccgtgt
     2221 tccagtttct ttatcagggg tacgacgcgg cgtcacagac gcgcgagaat ctcgcggtgc
     2281 tcgtgtccgg cacattcctc gcaaatacga catacgatac cgacctcgac acttccaacg
     2341 gcgtgaacac ctacgccgaa acgctctata ccgccggagc ggactgcggc gtcagcccgt
     2401 acattcttgc ggcgatgatg ctgcaggaga tgggcatgaa cggcgcgagc gacagcatca
     2461 gcggcacgaa ccgccgcttt ccggggtact ataacgcctt caacatcggc gcttacaaga
     2521 ccgcggagta ttccgccgtg gagcgcgggt tgtggtacgc cagcggcggc cataacggga
     2581 gcggcacctc gtgggggcgg ccgtggaaca gcctatataa agccatccgc ggcggcgcgg
     2641 ccttctatgc cgcgaactat gttgccgcgg gacagaatac gctctatctc aagcgcttca
     2701 acgtgcaggg cgagaacatg tactggaacc agtacatgac gaacgttgcg ggcgcggcga
     2761 gcgaggggcg gctgctctcc tatgcttaca gcgaggagat gcgcgcatcc aaactcacgt
     2821 tcaatattcc ggtgtatctc aacatgccgg agtctgcggt gcccgctccg acgggcgacg
     2881 gctcgccgaa cacgaaactt tcctcactta cagtgagcac tggcgcgctt tcgccggagt
     2941 tccggcggga tatacgggaa tataccgtca tcgttccgaa cgagaccgag cgcatcaccg
     3001 taacggcctc gccgctcaat gccgcggcga gcgtcgccgg gacgggtgaa tatgcgctca
     3061 atgtcggcgt gaacaccgtc acgctcaccg tgacggcgga atcgggcgca tcggagacat
     3121 acacgctctc cgtccaccgc cgcgcgccgg acgcgccggt gactatcacc ggcccctacg
     3181 ctatcagcgc ggacgggcgt gtttcgggcg tcgcgcccgg cacggcgctt gccgcgttta
     3241 cttcgtcgct cggcgtgagc ggcggaacgc tcaccgtgac ggacgccacg ggcgctgcca
     3301 aggcgccgga cgcgccgatg cgtaccggcg attttgtgaa gatcacgtat gaactcgacg
     3361 gcgcggaagc ggtgttcgct tccgggtacg tcgtcgtcgc gggcgacatc aacggcgacg
     3421 gcgccgtcac catcagcgac ctcatcaagc tgcgcaacca ccttctcggc accggcgagc
     3481 tgaccgggct gagtctggcc gcggcggacg tggaccgcag cggcacggcg gcgatcaacg
     3541 atcttatccg catacgaaac catctgctcg gcacggcgac gatcacgccg taaggagggc
     3601 ttgccgatga aacaaagaaa accgatcctc gcgctgctcg tcgccgcggc gctgctgccg
     3661 tggctcacgg tgagcgccag ggcggcggac acggacgtga tgttctccga tccgagcgtc
     3721 gttgtcggaa acaccgtcac ggtgaacgtg tatacgacct ccgccgttgc gggcatcgat
     3781 ctgacgctcg tgtacgacac ggactatctt acctacaccg gcgcatcggg cgggctcggc
     3841 aacgcggcgg tgcaggataa cggcgggagt ttgcgcatcg tggactacag cggcagcggc
     3901 gagggaaaat tctctctcaa cctgagcttc acggcccgcg ccatcggtac aacggcgctc
     3961 cgcccgacgg cgtgcagcgc gagcaacgcc gggggcgacg cggtgagcat ggagtacgct
     4021 tcccacagcg catcggtgac gatcacgtcg gcatcgagcg actgcacgct ttccgcgctc
     4081 tacatcgatc ccggcacgct ctctccggga ttttccgcca atgtgacgaa ctacgccgtg
     4141 acggtgccgt atgaaacaac atggctcgcc gtgagcgccg tgaagaacga cgcttcggcc
     4201 accaccgccg tgagcgggaa cggctcgctc tccgtcgggc tgaactatgt gaccgtgacc
     4261 gtcaccgccg ggaacggcgc gcagagggtc tatacgctcg ccgtcacgcg gcaggcgcag
     4321 gacaccgccg cgcagccgac aagcgacaac catgtcgata cgactgagcc ggagatcccg
     4381 gaggcgacgc ctgccgccgg tcggacgatc ttcaccgcgg aggggagaac gtacacgatc
     4441 ctcgacgctg cgatgctctc cgtccggccg ccggagggct gcgttctcgg cgattatgcg
     4501 ctgctcggca gcgtgtgccg ggcgtatctg ccggaggacg ggggagagta cgttgtgttc
     4561 tacgcgcggc ccgagggagg cgagccggga ctttatatct acgatacgcg cgaggaaacg
     4621 atgcagcgct acggccttat caccgtgccg gaggcgacac cgacgccgga accgtcccca
     4681 agcgcggagc cgacgccctc gcctgcgccg gagacgccgg atgcttcgga tgcggagatc
     4741 gaaaacctcc gctggacggt cacggtgctc gccgtcgccg ccgccgtgct cttcatcggc
     4801 gcggcggtgc tcggtacgct cctcatccgc agcatcaccc gcccggtgcg gaaagtaaaa
     4861 agataaaaac catgccgtat gcgtgcgatc tgcgcatacg gccggtttgc gcagagaaag
     4921 gaagagagaa atggccgatc ccgccagtgt gcgcgtcgtg gacgaagaga tttcgcttgt
     4981 gccgtattac ccgaatgagg aaacggcgct tccgtggtat caggatccgg acgtgtgccg
     5041 tcaggtcgat aacatcgacc atgtttatac gagcgagcgg ctcaacgcca tgtataagta
     5101 tctgagcaca cacggggcat gctattatat atcgtaccgc ggcgttctgg tgggcgacgt
     5161 gtcgctgcga aatagcggcg agctcgccat cgtgatctgc agggaatacc agaaccggca
     5221 catcggccgc cggtgtatcc gcgccatgct cgatcttgcc cgcgaaaagg gcatggagcg
     5281 cgtgacggcg aacatctatt cgttcaacac gcagagccgg aatatgttcc tctcgctcgg
     5341 cttccgggag accggcgggg agtggttcgc gctcgagctt tgaacgcggc ttttctggac
     5401 aaacggtgtg cgctgtgcta tgctgttata cggtggagta gacgttttcc gccgcataaa
     5461 gaaaggagaa tcccatgagc cttatcacac tcatcctcgt cctcgcggcg gcggccgtgc
     5521 tcatcgtgct cggcaccacg cgcagaaagg acggcggcga cgggaaaaag accctgccgc
     5581 gcacgatcgt gacggccgcg gcggtgctcg tgctcgtcgg cgcgctgggc ttcggctcgg
     5641 tgtaccagct gcaggaggac gaatacgccg ttatcaccac gttcggcaag ccgtccgtcg
     5701 tgagcacgtc cggcctcaag ttcaagatcc ccatcatcca gcagaaaacc atcgtcagca
     5761 aggcgacgca gggctttgct ctcggctacg atctgcgcac gaacgtgaac aacgaggacg
     5821 aatcgctcat gatcagcgtg gacttcaact tcgtcaacgt ggatttctat gtggaatacc
     5881 gcgtggacga tccggtgaag tacctttata acagcgaaga gccggaagcc attttgaaaa
     5941 tgctctgcca gagctacatc cgcgatacga tcggccttta cggcgtggac gatatcatca
     6001 cgaccggcaa ggcggagatc cagggcgtca tccgcgacaa gatcagcacc cgtctcgagc
     6061 aggaggatat cggtctcgtg ctcgtgaaca tcgccctgca ggacgccgag ccccccacgc
     6121 tcgaggtgca gcaggcgttc aaggcggtgg agacctccaa gcaggaggcg gagaccgcca
     6181 tcaac
//
LOCUS       DAWAAE010000123         6142 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5466_length_6142_cov_3.457368, whole genome shotgun sequence.
ACCESSION   DAWAAE010000123 DAWAAE010000000
VERSION     DAWAAE010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_6142_cov_3.457368"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3970..3979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1151 a         1901 c         1809 g         1271 t
ORIGIN      
        1 ctcaagggct ttgacgcagt atgagggcat ttccgcccgg cagaaccgcg tgtgctcttg
       61 tcaatcgacg ctatcataac gccaaaagcg gaacgacgta taacgccgtt ccgcttttgc
      121 catccgattt gccgccggga gagaggaccg gctgcgggct ggacgatcgc ccaagaaagg
      181 ggagaaatga gagggaaaga aataattgga ggttttgtct gtcttgcttc gtccgactgt
      241 ctgtatatta ccggaaatcc gcgctgtttt cctgaacaga ccgtgttatt tctgaaaaca
      301 aactgtgaat aacgcgtcct gtgcatgaag cagctcttgc cgccgaagcg gtttattgga
      361 atatgcaaaa agcctcgcag agtttgcgcc cgcaggcgca aagaaagttt agagcatttt
      421 ttccgatgac atgtgcatcg gaaaaaatac ttctcggctc gcaaacgtgc gagcgtaagc
      481 gagtgcgctg cggcgagccg ctatcccccc ctcaaacaaa cccctcaacg gagtggggtt
      541 tgtttgaatc agcaaaaagc ggaacgacgt aacgccgttc cgcttttgcc gtccgattcg
      601 cagccgggag agagaacggc tgcgggctgg aggatcgccc gaaaggggag aaatgagagg
      661 gaaagaaata attggaggga ttcatctgtc ttgcttcgtc cgactgtctg tatattaccg
      721 gaaatccccc gaagtttcct aaacagactg tgttatttct gaaaacaaaa tgtgaacgac
      781 gcggagggcg tgtataaatt cttttcgtac cggtaaaaat accctcgaat cccaaatcga
      841 ggaggttaac catggaggaa aagaaaggct tcagcaaaaa gctgaatgcg ttctttgccg
      901 ggaagggctt ctacatagtc cttcttctgt gcgccgtact ggtcggggct tcgttctggc
      961 tggttggacg cggaagcggg gctgatgtag agcgcgatcc ggtcaaggaa acgaacatca
     1021 cggcttcggc gcaggagacc gcgccgccgc agggcagcat ccccgttatg aacacggagg
     1081 aggacgagcg tctcccgctc ggcacgggcg tcctgcccgc cgcggagggc gaggagatgc
     1141 cggatgcgca ggcccccgcc gtgcaggagg agatgccgga cgttccggta tcggtgaacg
     1201 agagcgctcc gcagttcatc cgtccggtaa acggcgtgct gcttcgcggc ttcagcgcgg
     1261 aggcgctctc ctatgaccgc acgatggccg actggcgcgt tcaccgcggc tgggacatcg
     1321 catgcgccgc aggcgagccg gtgcttgccg tatccggcgg cacggtgacg gcggtgtatg
     1381 aggacgatct tctcggcacc gttgtggaga tcgaccacgg gaacgatgtc gtgagcgtgt
     1441 acgccaatct cgacgtctcc cccgccgtcg gcgtcgggca gacggtcgcc tgcggcgaca
     1501 cggtcggcgc cgtcggcaca acggcgctcg ccgaatccgg cgaggaggcg catctccact
     1561 ttgccatgcg gtgcggcggc agcaccgttg atcccgccga atatgtgccg gagacctgag
     1621 gcggaacttt tttcgggcgg ctgcgtctta tactaagacg cagccgttta ctttttttcc
     1681 gccgattgac gagcggcggc aaaacggcta tacttatgat ttgatatatt gctgtaccgg
     1741 gaggacgcca tgcatccgat cacaaagaaa cagaaaatac agtacatact cgccgccgcg
     1801 ggctttgtcc tgctggcgct ggcgctgctg tttctgcttt tgcagggtcc gtccggcggg
     1861 aaatccgacg cggacgcggc accgacgccc gaagtgaccg ccgcccccag tgcctctgcc
     1921 ggtctcacca tcgccggagt ggatgtcgat cccgcggcgg gcagctttga gcttgccgga
     1981 aagacgctct ctgccgggga ctgcgcctcc atcgcctcgc tcgggaatct cacgacgctc
     2041 tcgctgacga actgcaatat ttcggacgtg cattttcttg caggacttac gcagctgcgc
     2101 acgctcattc tctccgataa cgccatggcg gacgcttcgc cgctcgcctc gctctcgcag
     2161 cttcgcacgc tgtatctcga ccggaatccg gtcgccgacc tctccgtcct ctcgccgctc
     2221 tctgcgctca cgacgctctc cattcagggc gtggagatcg cggactatgt gctcgaagac
     2281 ctgcaaaaag cgctgcccgg ctgccgcata ttcagcgaca gcgtcgtgaa aggctcgcgt
     2341 ccggtgtctc tcggcgggct ggacttcacc gaatcggtcg aatcgctcga cctttcgggc
     2401 cgcggcatca cggacatttc cgcgctctcc ggctgctcgg agctgcgcga gctcaatctc
     2461 tcgaacaacc cgatcgccga tatctccacg ctcgccctgc tgccgaagct cacctcgctc
     2521 gatctgagcg ccacgggacg gagcgatctc gataacgagc ttctctcgtc gctgcgctcg
     2581 ctctcgtacc tgaagcttga aaacaacgac ggcgtcaccg ccgagggcat cgacgcgctg
     2641 caatcggcgc tgccgagctg ccagatcgtc catgagccga agtactacac cgtcgttttc
     2701 ggcgaccagt ccttctcgtc ggacgcctcc gatgtgacgg tggacgcgct gggactcaca
     2761 acgctctccg gcattgagaa attccaccag ctgcggcggc tgagtgccta cggcaacacg
     2821 ctcaccgacc ttgcgccgct cgcggagctg ttctccgtgg aggagttgca attgggctat
     2881 aacaacctct gcgatctctc cgctctcacc ggccacacgg cgctgcggcg actcattctc
     2941 gaccacaacg ccctgcgcga tatcaccgcg ctctccggct gcaccgcgct tgaggagctc
     3001 gatctcagct ataacgacct gaactccctt gcggcgctcc gctcgctcac gcagcttcgc
     3061 acgctgaaca tcaccggaaa tccggctctc accgccgata tggtgcgctc gctgcaggag
     3121 gctctccccg cctgccagat catcacggac atcgacctct ccatgccgga gccgacgccc
     3181 gaacctcccg cggaaacacc gcagccctga ccgtaccggt ttaaaaagca caaaaaccgc
     3241 ccatactatg tgtataacct acatagaagg gcggttcttt ttatgcataa aaacgacagg
     3301 ctgcggccat gggaaacggc gctgctcgcg gcggtgtgcg taacgctgct caccggcgtg
     3361 tgggcgcagg ggcggcagag cgcgcttgcg gacaaggtcg tgcggctgca cgtgatcgcc
     3421 gcgtccgaca gcgcggagga tcagcgcgta aagctcgagg tgcgggatgc gctgctcgcg
     3481 tatctcacgc cgcggctgga agccgcgtcc ggcgcgcagg atgccgccgc ggtgatcgcc
     3541 gcggcgtcgg gggagcttca gcgtatcgcg gagaccgcaa gcggcggcag cgcccgcgtg
     3601 gagctcgggc gtgaaacgta ccccacgcgg gaatacgaaa cattctcgct cccggcaggc
     3661 gtgtatacgt cgcttcgtat aacgctcggc gcgggcagcg ggcgaaactg gtggtgcgtg
     3721 gtgtatcctc cgctctgtac atcgggcgtg gagacggcgc aggaagccgc cgtgctctcc
     3781 gatgacgacg taaagctcat caccgaggac ggcgagggct acgtcctccg cttccgtctg
     3841 ctcgaatggt ggggctctct caccggaaag tgatctcgga ataccatttt ggcacgagcg
     3901 tccggaagaa atcgtagtcg cggatgatgc acgcagcgcg tgctgcggcg agccgcggcc
     3961 ttttcaaacn nnnnnnnnnt gatgcgcaca gcgcgcgctt tcatttcgga gacatccctt
     4021 gcaccaaacg gcttcgccca gacgatcgag aaaagcgccg cgtgggcgct gtatacggca
     4081 agcgccttcc agaagttttc cggcacgttt ccggcaaaat aagcgtcgat ctgtcccgcg
     4141 cagtacgccg cgctggcctc gacaccgaag cattcaagtt tatagaattc ctcgtacggg
     4201 tcaccaacct cccaacggtt gaagtcgatc acgccgagcg aaccgtccct ccggtacacc
     4261 agatttcccg gatggaaatc cccgtgcagg tataccggcg gctgcgtcca tatcgcgtcg
     4321 atattctccc gcacgaaagc gatggtgcct tcgtcgccgg gtatccgcac gtccgacgcc
     4381 tcgtagcgtt cgagctggcg gagcttcctt tccttttttg tctcctccgg cacatccgcc
     4441 gcgtccagcg gcagcaaatg gatcctgcgc aggatgccgc ccgcctcgcg tccgagacgg
     4501 tactgctctt ccttcggcag cgtgggcaga accttttcca gatcgtcgcc ctcgacccac
     4561 gtgagcagca tatacgtccc cgcagcgcac cgcccgaacc ctaccggctc ggatatcgga
     4621 aaaccgagag atgagaattt ttcgatgatc tcgcattcct ttttcttcgc cgcatatcgc
     4681 tccggtgcgg aaatacgcag ctgcagatac cgcccgtcct gcgtccggac atggtatttt
     4741 tcgtcgctcg accagccctt ttcgatcttt tccacagaca gccagctctc gctgccccgg
     4801 atctcttcaa acacactcat gccccccttc acggcgcaaa aggcactctc ttacgagagt
     4861 gccttttcgc ataatcaata ctggtaatgg tgcgcgtcct gcaagaccat ttccttcacc
     4921 ttcatgaggc gtacctttgt ggagttttcg ttctcctcga gcttcatgga gatgtatttg
     4981 atgttcttct gcagatcggg gatcatgacg tactcgagcg catttacgcg gcggcgcgtc
     5041 ttttcgatct cctgtgcgag aagctgcatc gtcttttcga cctgcgccag ctccagcatg
     5101 tcctcaaaca cgcccgagag agcggtcatg gcgtcgtcca gctcgccgga agtctgcgcg
     5161 agaccgtagg ggaagatatc tccttcctcg gcggttttgg tctggaaatc gtagaccggg
     5221 acgttgacgc tcatgatgtt cttgaagctc acatcgagcg ccacgctctg ccgcggatag
     5281 agcagcgcct gctcgagcat ttccggcgac atgaccgcgc tcgccacgct cagcgcgccg
     5341 aacgcttcgg tcagtccctg ctcgaccttc gtgcgcagct ccttattgcg gcggacaacg
     5401 tccataaact gacgcatcag ctcatcgcgc ttgtccttga gcagcttatg gccgcgggag
     5461 gcggttttca gtcggccctt catctggttg agcaccattc gggtcggtgt tatggttgcc
     5521 atgggatcac ctcttccttt ccgcgaagcg cattactgct gcttcttggg aagatacttc
     5581 gcaatgaact ccggcttgat gcgcttgagc tcacgctcgg gcaggatgga gagcagctcc
     5641 cagccaatgg tgagcgtctc ttcaatggag cggttggtct cattgccctg ggagacatag
     5701 cgcttttcaa actcttcggc gaacttcgcg tagagcttgt cgtcgtcgct cagcgccgct
     5761 tcgcccagga tcgtcataag ctccttggct tccttgccgc gggcgtaggc cgcgaacagc
     5821 tggttcatcg taccggcgtg gtcggcgcgg gtcttgccct cgccgatgcc cttatccttg
     5881 agtcgggaga ggctcggcag cacgtccacc gggggaacga cgcccttgcg gtacagatcg
     5941 cggctgagga tgatctggcc ctcggtgata tagccggtaa ggtcggggat cgggtgggtc
     6001 ttgtcgtctt ccggcatggt caggatgggg atcatcgtga tcgagccggc gcggcccaca
     6061 cggcggccgg cgcgctcgta catcgtcgca aggtcggtgt acaggtagcc gggatagccg
     6121 cggcggccgg ggacttcctt ct
//
LOCUS       DAWAAE010000124         6027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5574_length_6027_cov_3.106162, whole genome shotgun sequence.
ACCESSION   DAWAAE010000124 DAWAAE010000000
VERSION     DAWAAE010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_6027_cov_3.106162"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1401..1410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1655..1664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2404..2413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1202 a         1877 c         1698 g         1220 t
ORIGIN      
        1 ccgccggtca gcggagccgt gccgccgatc ttgaacgcgg cggaagccga atcttcgggc
       61 gcggaggcgg gggtggagcc gcagccggcg agacatgcca tgagcattac ggcggcgagt
      121 accatgcata ctactttttt cattgtttca tttctccttt tccaatggtt ttaaacacaa
      181 aaatgaatat aaaaacggcc ctgtccgaaa ggacaaggcc ggatttatca aaccggattt
      241 atctctttcg gtgctcggaa aaattgcgta caatcagaac gaaaataatc ataataatgg
      301 atacaataat ggtaataagc agaataagcc caaggaggcc aagggacagt cggaggctga
      361 gaagggcgga aataaaaaga acggacaccg ccgcagcgat gcccgcaata accggatagt
      421 gagaggaagt gcgcgcagca caagagccgt cgcaatttgc ggcggaagaa gcgctgttca
      481 tcgaaaaacc aaaaccgaag cagttcatgc ggcacctcct tcaaaagatg ggcatatcat
      541 agcacgctgc ataagtatgc gtcaatagaa aaatgcgaaa caatttaaaa tgctaaagcg
      601 taaatttttg gaatttttca caaacgcgcc agtttctttt cggcaaaggg gagaaaaaaa
      661 cagtatttcg gcagtagacg cggcgcgaaa acgctgatat aatgccgatg tgcaaaagag
      721 cgtatccata tactttatat aacggaggta aatatcatgc cgatcgtaac cacaaaggaa
      781 atgttccgca aggcgtatga gggcggctac gccgtcggcg cgttcaacgt caacaacatg
      841 gagatcgtcc agggcatcac cgaggccgcg gccgaggtca aggctcccgt catcctgcag
      901 gtctcccgcg gcgcgcgtgc ctacgccaac cacacctatc ttgtcaagct cgtcgaggct
      961 gccgagcagg agaccggtct tccgatcgcc ctgcatctgg accacggcga cagctttgag
     1021 ctctgcaaga gctgcatcga cggcggcttc acctccgtca tgatcgacgg cagcagcaag
     1081 tcctttgagg acaacatcgc tctcacccgc caggtcgtcg aatacgcgca tgaccacggc
     1141 gtcgtcgttg aggccgagct cggcacgctc gcgggcgtgg aggacgacgt ccacgtcgag
     1201 gccgaggaca gctcctacac ccgtcccgag gatgtcgagg aattcgtgac ccgcaccggc
     1261 tgcgacagtc tcgccatcgc catcggcacg agccacggcg catataagtt caagcccggc
     1321 acgaagcctc agctccgctt cgacattctc gaagaggtcg cgcagcgtct gcccggtttc
     1381 cccatcgtgc tgcacggcgc nnnnnnnnnn agatgctgcg ccaggccgct accatgtccg
     1441 tctgcaagat caacatcgat tccgacctgc gccttgcaat gaccgcctcc atccgcaagt
     1501 actttgacga gcaccccgag ggcttcgatc cccgcgccta tctcaagccc gcccgcaccg
     1561 ccattcacga catggtccgc cataagatcg tggacgtgct cggctgcgac ggcaaggcct
     1621 gatcgccgct tttcccaaaa tccaagcgcc ggggnnnnnn nnnnggcgaa cgccccggcg
     1681 ctttccatcg tgaggtcatt ttatgccgtt taccggattt acgagcgaga ccggagaatt
     1741 tctctgggaa ctcgcgttcc acaacgaaaa accgtggttt gaagcgcata aggagcagtt
     1801 tctgcgcgcg gtaaaggagc cgttcgacgc gcttgcaaag gaaacgctcg cgcttatgcg
     1861 ccaaaagcac ccggacgagc cgtactgcct ccacatttcg cgcatctacc gcgacgcgcg
     1921 gcggctcttc ggccgcgggc cgtacaagga ccacctctgg ttcactctct ggacgggcga
     1981 agatcggcac aacagccccg tgttctggtt tgagctctcc cccgcctcct tcagctacgg
     2041 cgtcgggttt tacaccgcga ccgcggagca gatggcggcg ctgcgccggt acatcgacgc
     2101 gaatcccgcc gagatggagc ggcttgccaa gcgcatggca aaaagcgaat tccatctcga
     2161 aggcgagccg tacaagcggc tcaaaaagga tgtgggcgaa gtgctcaatc cgtggtacaa
     2221 ccgcaaatgg atcagtctct gcgccgaata cgaccatggc ggggcgctct acacaggcga
     2281 gctgccgcag gtgcttctgg acgcctacga aaagctcatg ccggtgtatc ggctgctcaa
     2341 gagattcccg gcggagagca gcaatgcctc ccttgtgtaa agggaggtgg attttgccgc
     2401 aggnnnnnnn nnnttgtcgt tttccagaaa ctacaatccc ccagtcagct tcgctgccag
     2461 ccccctttac acaagggggc ccttcatttt actcggagat tatgaccggc tccccggcgc
     2521 agcgcagctt cccgccctgc acggtcacgg tctcgccggt gatatagttt acataatctc
     2581 catccttcac cggcacttcg acctcggcgc tcttcccctt cagagagaag acgccgagct
     2641 tttttgtttt cccgtcgtcg cgcaggcaga cggcaatgtc gttcgcgtca tccgcttccg
     2701 cggtgaacca gtcctccgcg gagagatgct cccgccgcac ggcgttgagc ttctgcaaaa
     2761 gcggcgtgat atccttgcct gtgcgcggga acacgtcctt atcaaaaaga ctcggcacat
     2821 gatcgcagga atattcctgc ccgccgtaga gcagcaccgt acccttgagg aagtacagca
     2881 gcgccgtgaa attccggcgc gccttctcgt ccggcacaag accggcgatg cgcggcatat
     2941 cgtggttttc catgtaccgc agcttcacat agttttccgg atagatggcc tcctgaaagc
     3001 cgagcatgtc catccacgcg ctgagcggct gctccccggc gatgtactta tcgagcgtct
     3061 cgcggatgtc gtactcatat tccatgtcaa acgcttcgta aagctcgtta tcggtcgccg
     3121 cggtgatacc gcgcagccgg ttgaacacaa gaaagctccg gtgcaccgat tcggcgagcc
     3181 agatgcaccc cggatgcacc ttttccacgg cctcgcgcgc ctttttccag aactccaccg
     3241 gcacgagcga ggcaacgtcg caccggaagc cgtccacgat gcccgcccaa tagcacagat
     3301 gctgtatctg cgcgtcccag agcgcggcgc aggaatagtc cagatcgata acgtccgtcc
     3361 agtcggcggt cttgttgccg aagcgtccgc ctggcttccg gtaataaaac tccgggtgct
     3421 cggtgacgta caccgcgtcc ggcgacgtgt ggttgtacac cacatcgatg atgcacttca
     3481 tgccgcgcgc gtggatcgcg tccacgagcg ccttgaaatc gtcaagcgtg ccgtactcgg
     3541 gattgaccgt gccgtaatcg cggttcgcgt agggacagcc gagactgccc tttttgccct
     3601 tctcgccgat ggggtggatg ggcatgaacc agacgatatc cgttcccagc gcgcggatgc
     3661 ggtcaagatc ggggatcacg gcgcggaacg tcccctccgg cgtatggttg cgcacataca
     3721 cgctgtaaat gacctgttcg cgcagagccg tattcgtatt ccttgccata gcgatttctc
     3781 ctttgcaaag cacgccgccc cggagccgac aggcaccggg acgggaattt ttttatttgt
     3841 tttccgcagc cttcaccgcc gcgcagatga gctgcacgca cgcttccaca ccgagagagc
     3901 cgctgttgag cgtaatgtcg tagttgtggc agtcgcccca gtgctggccg gtgtagtgct
     3961 catagtagct gcggcgggcg cggtccttct tcttcatcgc gcggacgatc tcggcggtgt
     4021 cggtcgtgtt cagatagccg gagatacgct cggcgcggtg cgcctcgtcg gaatagatgt
     4081 acacattcag cgtctcgatc ccggcgtcgt gcagcacaac gtcggcgcag cgcccgagaa
     4141 tgacgcacgg tccctgcttg gcaagatcca gaataacgct gcgctgggtc tcataaatat
     4201 aatccttctg gtcataggaa atgggcgtga tgttgcgcag aatgctctcc gtggcggaga
     4261 tctcctcgcc ctcgcgctcg atctggcttg catcgatgct gctcgccttt gcggtggcgg
     4321 caatgatgtc gcggtcatag agcgttacgc cgagctcttt ccccaccgcg agaccaatct
     4381 cgcgtcctcc tgcgccgtac tgccggctga tggtgatgat cttcatagaa ccgccgcctt
     4441 tcatggtttt tctttttata ccatcaatcc gcataaaatg caatatgctg tcagagaatc
     4501 cgcccgtctt tcagcgcaaa aatggcgtac tccacgccgt ggatcgtccg cttttccacc
     4561 atgcgctccg tcggcagtcc ccacgccgcc atggggattg cgcggtacgg ctggttttta
     4621 tacaccgtgt acaaactccc gtccccggcg tcacgccaga cgatatcccc tgcgcgctcg
     4681 cgcggcttcg gcgcggcgga ctgcttcggc gtgagaggga cgggcgcggc ctgtgccgga
     4741 gccgtctccg gtctcttctc cggcgtctgc ccgctctccg ttctgcactc cggcgcagag
     4801 gtattccgcc cggcttcggc ggcgagctca atctccgccg ggaacgccgc aagcgcggcg
     4861 cgcgtcagcc gccggtgcag cgtcagcaca ccgttctccg gcgtcatcac gccgagatag
     4921 ccctcttttc cgccgccgta tacgctcagg cgcacgagcg tgccggggtc ctcgcacttg
     4981 gcgtcaaacc gcgtcgtcag cccctcgcgc gaaacggtca gcgtcccctt cttctccccg
     5041 tttatcagga ccggatacgt ctcactcatt ttatcactcc atccatattt tacgctccct
     5101 ctgggtctcg cagagtttgc tcccgcagga gcaaataaag tcgcatccat tttctccggc
     5161 gacatgcgcg tcggagaaaa tactgctcgg ctcgcaaacg tgcgagcgaa gcgagtgcgc
     5221 tgcagcgggc cgcattttcc ctcaaacgaa cccctcaacg gagtggggtt cgtttgacga
     5281 aaaaacaccc cgtccatatc ctatggacgg ggtgcggctg ttatgccgtt attggggtat
     5341 cgttccgccg gggcgtccgc cgccgagacc gaagctcacg gcgatcgcgc cgttcacgcg
     5401 atccatcacg gcgtcgggca gcttgcccat acgctcgcgc aggcgcgatt tgtcgatcgt
     5461 gcggatctgc tcgagcagga taacgctgtc gcgcgtcagg ccgcagctcg cgccggcaag
     5521 ctcgatgtgc gtgggcagac gcgccttgcc ggtctgcgag gtgatggcgg cggcgatcac
     5581 ggtggggctg tggcggttgc cggtgtcgtt ctgcacgatc agcaccgggc gcacgccgcc
     5641 ctgctcgctg ccgacgacgg ggctcaaatc ggcgaaataa atgtcccctc tccgaactgt
     5701 tctcatgcga ttcactctcc agatgaaaat gctcgcccga tacatcttat gtagggctac
     5761 actcattttc cctcgttaag agtgaaaata tacgcatacc ggagttttaa tctttatcgg
     5821 gggcgaggcc ggtgcaggtg acgctgtcgc ccgctacgac gcggccgcgg atattgccgc
     5881 agcggacgcc gtccccggca gtcacgccgc cctcgacgtc tctgcactcc acgctgtccc
     5941 cggcgtgtac gttgccgtca acgttgccgc aggtcacgct gtcgcccgcg ttgacgtgcc
     6001 cgagcacatt gccgcactgc acgccgt
//
LOCUS       DAWAAE010000125         5985 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5623_length_5985_cov_3.104384, whole genome shotgun sequence.
ACCESSION   DAWAAE010000125 DAWAAE010000000
VERSION     DAWAAE010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_5985_cov_3.104384"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1374 a         1681 c         1755 g         1175 t
ORIGIN      
        1 gccccttcaa acgagagccc aacgcagtgg gtctcgtttg agagcgtgtc aaaaacactt
       61 ttttgacacg ctcaatccgg ctttccaacg gaaagccgga taaatcggat aggatccttt
      121 taaaacagcg gcgcgagcgt gcgcagcacg gcgcggatca gccgccgacc gcggcgctgc
      181 tccagacaca gctcctccgt gatgggcgcg ctccgttcca gctcggaaag gtattccgcc
      241 ttcatgtccg cgatggcggg cgtatcacac atccatacgc cgcactcaaa gtggagatac
      301 aaactgcggt aatcgagatt gatcgttccg accacggcgc tgcggtcatc ggcgatgaac
      361 gttttggcgt gcaccatgcc gggggtgtac tcatagatct tcacacccgc gagaatgagc
      421 gtttcgtagt aggagcgggt catttcatgc acaagggcgc tgtcgctgat cgctggagtg
      481 acgatgcgca catccacgcc ggacttagcc gcggcggtca gcgcggtgat catctcgtta
      541 tcgatgatga gatagggggt gttgatatac acatagtctt tggcgcggtt gatgagatgc
      601 atgtacaccg tttcgccgac cggctcgttg tcgagcggcg tatcgccgaa cggctgcacc
      661 cagccggggg cggtgatgct ctcgcagagc gccgcgtccg gcgcgtagcg ggtgaaatcc
      721 tccgttacgc cgcgcgtgta gtcccacatg gagagaaaca tcgccgtgag cgcgtacacg
      781 ccctcaccgc gcagcatgac gcccgcgtcg agccagtggc cgcagcgctg caccacgttg
      841 atataggcgt ccgagaagtt gatcccgccc gtgaagccga cattcccgtc gatcacacag
      901 atcttgcggt ggtcgcggtt gttgaaccgc ggcgagagaa tggggataaa ccggttgaac
      961 acgcagcagc ggatgccctc ggcctcaagc gtgtcctcat agccctccgg cagcttgtac
     1021 atgcagccga aatcgtcgta catcacgcgc acatccacgc cgctttccgc cttgcggcgc
     1081 agcacctcgt gaatggcgtt ccacatcgtg ccgggggcga tgatgtagta ctccatgaaa
     1141 atgaacttct ccgccttttc cagctccgcc agaagcgtgt caaaatagac ctcgccgacc
     1201 ggaaggtacg ttgtttcggt atgcgtgaat accggcgcgc cagcggcacg gtgcagatag
     1261 tcggaaacgc gcttcgcgtc cgtgtgctca ggcagcgccg gttccgccgc cggaacgctg
     1321 cgcatcgcct tttcatactg ctcggccacg gatgacatgc gcgccttttc cttcagcgag
     1381 agctggtttt tcccgaatac gagatagagc agcacgccga acaccggcag cgcaaggatc
     1441 ggaatgatcc aagcgatctt gtacgcgggg ttgccgtcgc ggctgacaag atagacgaca
     1501 acaacggcgg agagaacgag gcagacaccg taaaagatgg caaagtgctc gttgaaccgg
     1561 gtgatggcaa ggtagacagc ggcaaactgc agcgcaatga atgcgccggt gaatacggcg
     1621 cgggaaaata cgactttaaa aaagttcttc atgggcttct ccgcagaagg ataaatttct
     1681 ttactatatc atacacgcgc ggcgcggaaa attcaacaac agatacgcca gtttgtctta
     1741 cgcggctgct ccggacagca gcatgaacgc catggtcagc agcagaagaa gaaaccgacc
     1801 cttttccacg ccgagcttca tcatcagcgg aagcgtgatg agaagataga cgacggccga
     1861 cggtgcagta acggcgagag aggagagaag ctcgccccgc ccggtaagaa gggcggcaag
     1921 cgtctgtact gccgcgccga gcgcgatggc gatcacaccc agcagaaacc ggctccagaa
     1981 gaggtcactc ttccgcaccg gcatggcgag cgcgtaccgt tcccaccggc agcgttcgtc
     2041 atagccgagc gcatagaccg gcatcatcgc aagcagcatc atgccgacgc ttgtaaagag
     2101 cggcaggagc aggtacacgc ccgtcagaac gagctgcacc cggcactgcg cccacagctc
     2161 aaatatgtcc ttgagcaaaa gtccgcggat cttcataacg cccgctcctt tctcgtgatg
     2221 tccagaatga tctcttcgag cgtttgaccg ggatgcgccg cgagaagggc ttccttttcc
     2281 tcgctcagga tcagccgccc cttatgcaga aacgccacac ggccgcagag cttttctata
     2341 tcgctcacga tatgcgagga gagcagtatc gcgtgctcgc cgtctgagat aaagcgggag
     2401 agctcgccga tcagctcgtc gcgggcgagc gggtcaagcc cgccggtcgc ctcgtcgagc
     2461 agcaggagct ttgctttgtg cgagagcgcc acggcaagcg cgagcttcat tttcatcccg
     2521 cgggaatatt ctttgaatgg cttcgtttcc ggcagatcaa agcgccggat atgctcataa
     2581 aatgcgccgc tgtcccaatt tttatagatg cagcgctcca ctttgtccac ctgccgggcg
     2641 ttcagcccgt ccgggaagca gctctcgtcg aatactacgc cgatgtcctc gcggacggag
     2701 acgctgtcgc cgtctgtgtc ttgcccgaga atgcggatct ctccgctgtc gcgccgaagc
     2761 tgcccgagaa tgagcgagat cgtcgtggac ttgcctgcgc cgttttcgcc gataagcccg
     2821 agcgccgtgc ccatcggcac ggtcagcgtc aggttttgca gcgaaaagct gccgaaggat
     2881 ttggacaaat ccttaatttc caatgcgttc atgggatttc ctttcagtat ttccggttgt
     2941 agtaccagaa gcagccggta tagacgagcg tctgcaggca cgtgagcgcg cagagcacct
     3001 gtccgaccag agtatgttta aagatcaaaa tagcggcgag agctgcggcg agctgtacat
     3061 aaatgcagat gtaaagcgcc atagaacgcg ccttgtttgc cacatacgcc gcgcgctcgt
     3121 cggtcagcga ggcgtcatag tccgccgctt tgtccgggtc acgggtgagc cggtacacgc
     3181 gcagcagccg cgccgcgccg acaacggcaa tggcgctgcc gaacgaggaa agccagtttg
     3241 ttttttcgcc ggcgtctgtc agcagcccga caagaaacaa tgccgcgccg agaacaagat
     3301 ataaaatacc gagcttcttc tgatccttga gcttcatgct tcatcctcct caaacagaaa
     3361 aatatcctca atgtgcaccc cgaacgtctg cgcgatcttg tgcgccagca atatcgaggg
     3421 gttgtacttt ccgctctcca gcgaaatgat cgtctggcgc gaaacgccga gcaatgcagc
     3481 cagctgctgc tgcgtcatgc cctgcgcctt gcgccgctct tcaatacggt tcttcatcgg
     3541 gtcacctcaa cgtgaaaaag taaagcttgc tttacatacg cagtatagca cggcaaaatg
     3601 aaatgtcaag catgttttac attttacttt ttattttttc ggaagtcctg attataggac
     3661 actcgatgca gtaatataca accataagag agataaaact ggaaaggaag agcgatatgg
     3721 cgttcaagat cggtttttct gtatcggagg aaaaggaaaa gatgaccgct ccggagcctc
     3781 cggacgttgc ggaagagcgc gagccgcggc ggtcggtggt gcgcgtgcaa ttcccgcagc
     3841 ggaatatgac gcttgcctac tataacgacc ggttcgacct gcactgcggc gatctggtgt
     3901 acgtagacgg caagctcgag ggaaagcgcg ggcgcgtcgt agatgtcaac tacaacttcc
     3961 gcatcaatct ggcggactat aagcgcgtga tcggcgtggc ggataccaat gtgtccggcc
     4021 ggtttaccct cgccgggtcg cacgttctga cgttcgaccg cgcggcgctt ccgcgccgga
     4081 aggtgatgag ctggtataaa gcgccgtcga agcctgacac ggagtatgcc tgcggctcgg
     4141 acgacagtac gttcccgctg cgggagctgc accggatgcg catcgatgtg gcggtcgcgg
     4201 agcgcgggta tgcgtactat gcggaaaacc gcgtgcgcta tctctgtctg gacggaatga
     4261 aagggtacgc gcttgtcgag ggaacggagc cgtatgacgt ggagttcgtg tattcggacg
     4321 gcatgatcta cgcgctgacg tgctcctgct actgcgtcgg aaactgcaag cacgaggtcg
     4381 ccgccatgat gcagctgcgc gaaatgctcg actgcattga ggaaaactat gcgggtgaat
     4441 atgctgctac tggctacttt gccgccgttg caaaggatgc gcttttgtcc cttgttgtta
     4501 acgacaaaac gaccggcacg ctcacgctgg agccccgcgc ggcagatgag ccggacttcg
     4561 atgcagaatt cgatggcgga gatgaggagc gggacgattc tgattggtga gacggaggca
     4621 gaactccgtg taggagagtg ccccggcgcg gacgatggcg gacgtgctga ccggaaagaa
     4681 cggtgatacc gaaaagccga gttttctaaa gaagaaaaaa ttgccgacaa agtatctgat
     4741 accgatgtgg cggcgcaagg ccgcgcaaca ggccaaagcc ccctcggcaa ccacgtccaa
     4801 gacgcagata cgaatgtcag caattcggaa gcacccgctt ccaacgctat tgtaccggct
     4861 caatcgccga atgtcaacac ggaaggcgtc gaaacgcgcc tttcgcagac cgtcgagacg
     4921 gtgcgcgacg ctgccgttac tccggacgcg gtgaagtaga ccataaatga gaatatcgaa
     4981 aacggagggc atcggtatat cccggaatcc aacgccgacg cggtggaaga gatgtatatc
     5041 agagaattat aaatactttc ctttggcggg gaagaaaaga taacgtggag cagcccggac
     5101 aaacgtccgg gctgctccat tgcatattac tttaatccga gaatgaagat caccgccgcc
     5161 atgaccggca gcacggtcca tgtgatatac cgcccggcga tctcccagtc ggatggctcg
     5221 gcttcctcgg cgttgcggac aaggaacgaa gcgttccagc ggaagagctc atcggcaaac
     5281 agcatggcga gcgccgcgat aacgcagatc agcacgccgc cgaaccaacc gagccacgtt
     5341 cccttatggg tcagcgctgg gccgtccgtc agatcgagga tcgttgaaac agagggctcc
     5401 atggtaccgt cgctcatcgt ggcggtgatg ctgcggagca ggtcaaagct cccgtcctcg
     5461 ttatagagca tacgccagtc gccgctgttg actacgccgc cgcggaacac gatctcgccg
     5521 ccgcagcgca gctcaatgcc ggtcatctga gtttgcaggt cgctgtcctt cggaatggcg
     5581 gtgggatcct ctttgacggt ataggggccg taaacggtgt tgccatactg aaagatgacg
     5641 gttttattcg cagatacggt gaaggtcgcg gcggttccgc ggatcttccc ggaataaacg
     5701 gtgccgccat cctcttccgc cggcacgaga atggtgtcct cataaagaaa acctgagagc
     5761 gccgtaacga tgggatagat gatcgtaaag gccaggatca taaccgccat gagaagcagg
     5821 aggatctttt gatagcgtcc aagcaccttt acccgtgcca agtctgacac ctccattttt
     5881 tgaagaatgg tggaataatc aaaaaaagca tagcatctcc gcccgaataa aaccaaatcg
     5941 aagcccagcg aagcgggttc gatttggaaa ggaagaagga gctaa
//
LOCUS       DAWAAE010000126         5919 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5684_length_5919_cov_3.395634, whole genome shotgun sequence.
ACCESSION   DAWAAE010000126 DAWAAE010000000
VERSION     DAWAAE010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5684_length_5919_cov_3.395634"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1100 a         1690 c         1718 g         1411 t
ORIGIN      
        1 gggtgtcatg gttgccggtg atggtgacag tcacttcgtc ggtgttcggc gtgatcgtca
       61 gcgtgccggg ctgataggtc ggcagttcat aatagccgcc gggcagcgcg ccgcccttga
      121 acaggacagt ggtcgtatcg atgacgttcg acacgctgcc gacattggtg atggtgctct
      181 ccttcgtcat ggagagggtg atatccgtct tctgatcgcc gttgacaagg ccctcgacct
      241 tgaagtccgg ctgcgtgagc ggctgggcgt tgtaggctct gctcgcgctc tctgcggtga
      301 tcgtcagagg aacggtgtag taaacggtga tgacgttccc cgtcgctttc atttccagcg
      361 actggctggg ggagtagctg ctcgcatgcg ccgacgtata ttccgcaccc agcgcggtcg
      421 aggcgctcgc cgtcagcgta tcgccgatcg tcatggtgcc ggtctggtcg ggagcgacct
      481 tcacttccgt acccttgagg taatggtgga tggtgtactc cgcggtgttg cgcttgaagt
      541 acagcttcag cgtcagcgag ccgtccgcgg ccacggtgcc ggactcgagg ttgttcgtgt
      601 tgccctcatc gtacacatac gtgggcgtgc cgtatccctt gtctgtatcg gtgacggaaa
      661 cggtggcgtc cgtcgtgccg gtgcggggat cggtctttgc gagcgtgtag gcgccgtcgg
      721 tctgctcgca gtagaactcg accttatagg cagtattctc gttcgccttc cacacagcgt
      781 agaggatatt gtccccggcg ctgtccacgc gggcggtgct gccggtctta tattccgcac
      841 tggcagcgtt cggatcggtg ctccatccga cgagggtata gccggtgcgg gagaagccgc
      901 tgccgtccca gagcgtcaca agaccgttat tgacgatctc tttttcgacg tggtctgcgc
      961 cgctgccgcc gttggcctta taggtcaggg aaacgaccgc gtccttgttg ccccaaacgg
     1021 catggagcgt gacgctcgcg ttcatgcgca gcttgtcgtt gggctggtac ttgaccgccc
     1081 cgtccgccgt ctctgcccag ccgaggaaca ccttgccctc gggaacggcg aaggagttgg
     1141 cctgcacgtc ggcgtgcgca ccgggggcat agcgcttcat atccgtgaca gtattctttc
     1201 cgtcagtata tgtcacggtg aagctgttct tattggtata gtaggggtaa agctcgatat
     1261 tgtcgtaaat tttggtgctg aagtggaagg gcgtgtaagt atcgccgttc ttcgtcgccc
     1321 agccagcaaa tgtccagccc tcgcccgccg gcttctgcgg atcggggagc atgctctcgt
     1381 caatggtgcc gccgtagggg atggtgagat tctttcctcc ggtgccttcc atggtgatgt
     1441 aggcaacgcc gcttaccgtg ggcgctgccc acttggcata gacggtgatg ttgttggcgg
     1501 gcatggtctt gctgtcaaag tcgtatacct ttgtcaaggt gggatcttca taccagccgc
     1561 cgaacgtgta ggcttctttg tctaccggct tggtcgccgg cgtatagctt cctgcgccgg
     1621 tcaagccctg ctcatattta tatgatacgg tgtttgccgt ctgaccgccg cttatgtaca
     1681 ccacattgta gctgttgcgg gtgtaataga atctggcgtt gttgtatttg ccgccggtct
     1741 tcgtgctgac accggagttg aatgtaaagc cggtgatggg gtactggtcc tcgctagtga
     1801 ccgtgtagcc ggtgccgggg gaagtatcgg aatgatgcag cttgtaggac ttgccgccga
     1861 cagtggtcgt cccgctctcg ccgggcagga cttcgacata atagtacgcc gtttccgagc
     1921 cgctcccggt tttcggtcca tagaatgtgg cgccgttcag cggcatcgta tcgatgttga
     1981 cctgataggg gccgctgcca tcactctgcg ttgcccacgt gctgcttccg tcgtaggtgg
     2041 gccacttatc gccgatggcc gcgccatatt tggcggtgat gcgcaggtca gcatactcct
     2101 ctgatgaact aaaccatccg gaggtgctgt agaactttac ctcgtacaca ttgcgcttgt
     2161 agtatatgtt gacgatggtg gagccatcgc ctttgatggt ttcctgcgag atcgtctggg
     2221 gggtgaagcc ggtgtagctc ttagctctgg cgctcgtctg cgcgccggtc gtaccataca
     2281 gcgtctcgtt ttccacataa gagtagccgt cgtcatcggc attctcctgc cagtggatga
     2341 cggtgtactg cgtgtcggct ttggcgttcc agcgcgcctt gagcgtgatg ttctccgtca
     2401 gtgggctgcc gaattcgaac ttcgttccgt tgtattccca gtggctgaac gtgtagccgg
     2461 gcttcgtcac cacattggga gctgccgtgg ttgcgtctgc gctgtagaac tggggcgcga
     2521 catatgtacc gccgtcggaa tcgaagtgga gccagtggcc cggttcgacc ttggcgtaca
     2581 cggtgatatt ctctttgtcc agcgtgacgg agtcgacagg ggtctggtag ctgggttcca
     2641 gataccagcc ggtgatcgcg tgcgttgtgt tctcaagacc gtaggttaca tcgtcagtct
     2701 tgataatgtc gcctttcacg ccctctttgg tatggacaat gcggccgttc gtgtcggcaa
     2761 agaacacgta cagcacttcc gtgaatccgg cgtccacgtt gatctgcgcc gattcgctga
     2821 tctcgcccac ggtaccgaag gcgagctcgg acgcgccgac gaaccagccg gtgaatttat
     2881 agccgtcctt cacgggggaa ttgggctgat agagcgtgtc gcccttcttg acgatctggc
     2941 gcgagacttc ctcgccgtct acccgaaaga cgtaggtgag gtagttgccc gtatccggcc
     3001 ggacaactct catggggaca acgtttgtct tgccgccctc ttcgtcctcc gcgggctctt
     3061 cgtccttgcc gggctcttcg tccttgccgg actcttcgcc cttcgcgggc tcttcgtcct
     3121 tgccggactc ttcgtccttg ccgggctctt cgtccttcgc aggctcttcg cccttcgtgg
     3181 gctcttcccc cgcaatgggc ttttcgcctg ttcccggaat ggagtctttg tctccggcgg
     3241 ggtcggtgct gccagccggc gcggaatcgt ttccattttc ggaaagaagc gtttcctctg
     3301 ccgggatggt cccctcgtct ccaacggcga tcgcgttcac cgggagcagg gagaacatca
     3361 tgcatactgc catcagcagc gcgatcagat tcctcttttt catacagtcc tcctgttttc
     3421 acttacctct ccctcgttgt cggacaattt cgggagaaaa tgtacggatt tttctgtgcc
     3481 ggaacatggt ttttctgcgc attggtatag ttatcctact gtgttttggt atagttatcc
     3541 cactatgttg ttttcattct acaagatacc gtggaatttt tccataggtt tacgtaacat
     3601 ttcattattt gtaacaaatt cttaacagtt tttaacattt tgtacaggta gtgattgctt
     3661 atcccagaaa ccgctatacc ttgtggttcc cccggtattg ccggaagcga tttaaaatgc
     3721 cctctcggca gccgagaggg catattcccc tatactatta tattatagac cccgcagggt
     3781 tattcctctt tttttctctc gcgcgttttg acgcgctcgt gcggcgtggg gccccagccg
     3841 tcgagcggca ggcgctgcat cgcgccgaac accttgctgc gtatatccgg gtactgcgcc
     3901 gagagcgtcg tgagcagatc cttgatctcc tgccgcttgg acgtgtgatc catcgggcag
     3961 gtgctcttca cgaccggcag ctcgagctgt ttggcgagat tcgcgctgcg ctgctcgccg
     4021 atgtacagaa gcgggcggat ctgcgtcacg tccgcgcggg acatatacgt caccggcttg
     4081 aagcagttga gccgcccctc gaacacgagc gacatcataa acgtctccac ggcgtcgtca
     4141 aagtggtggc cgagcgcgag cttgttgcag ccgtgctcct tcatcgtggt gttcagcgcg
     4201 ccgcgccgca ttttcgagca gagcgagcac gggttctcct cctgccgggc gtcaaacacg
     4261 atctcgcgga tatccgtcgg cacgcgggta tacggcacgc cgagctcctc gcacattttt
     4321 tccacaccgg aaaagtccat gccggggaag ccggtatcga tggtgatgcc ctccagctcg
     4381 aacggcgcgg ggtaataccg ccgcagatac gcgagcgcgg cgagcagcac gagcgaatcc
     4441 ttgccgccgg aaatgcccac ggcgatgcgg tcgccggggg agatcatgcc gtaatcgtcc
     4501 acgcaccggc gcacaacgcc ggtaaacgca ttgagagaat ccatatttcg cacctcaaaa
     4561 attaaaaatc gaaacagaga aaagcggagc atttgcgaga aaaacgaagc atttacgagc
     4621 ttgccctgcc gtaaataaaa atttcggaaa acaggggttg acaccgacgg agcgctgtgc
     4681 tataaaaata gagcgcgtct tgagggtata ttgtacccgc cgcgccttga aaaagcaaga
     4741 aaaaacaatg atattggagg cacatcaatg tccactacga tggtcagaaa agaggacgtc
     4801 gtccgcaagt ggtacgttct cgatgcggcc ggcaagcccc tgggcaagac cgccgctctc
     4861 gccgccgacc tgctgcgcgg caagcgcaag gtcgactaca cgccgcacgt cgactgcggt
     4921 gatttcgtca tcatcatcaa tgcgaaggac gccgttctca ccggcaacaa gctcacccag
     4981 aagtactacc gcacccactc cggttacgcc ggcggtctca aggagacccg ttacgatcgc
     5041 ctgatggcga cccgccccac tctcgccatg acgctcgccg tccgcggcat gctggcgaag
     5101 aaccccaccc agaaatggca gctccgtcgt ctgaaggtct ttgccggcaa tgagcacaag
     5161 cacgccgcgc agaagcccga agtctggacg gtcgaataag gaaaggagga aattttagaa
     5221 tggctaccta tcagagcaag cgtccctacg cttacggcac cggccgtcgg aaatcctcca
     5281 ccgcccgtgt ccacctcatc cccaacggca gcggcagcat caccatcaac ggccgcacgc
     5341 tggacgaata cttcggtctc gagactctga agctcatcgt ccgtcagcct ctcgccgcca
     5401 ccgagacggc cgagaagttt gacgttgaag ccaccgtcgt cggcggcggc atgaccggtc
     5461 aggccggcgc gatccgtcac ggcatcgccc gcgcgctgct caaggtcgac gagaactacc
     5521 gcggcacgct caaggccgcc ggttatctca cccgcgatcc gcgcatgaaa gagcgtaaga
     5581 agtacggcct caaagccgcc cgtcgcgcac ctcagttcag caagcgctga tcggacgcaa
     5641 atttacaact caaaagctca aaaaccccgg aaaatcagca ttttccgggg ttttcttata
     5701 cccttttcgt ctgattgctt ttgaagcctt tcgttgtttt tgaccgtatg tgacctcggt
     5761 taacccattt tataagggct aaaaatgggt taaccgggac aaatgggtga gtaaatgggt
     5821 gagtaaatgg gttaactgaa gggtcaacac aaggtgaatt tgcgggtttt tttcatgaac
     5881 gcccctaagt aaaaatgaac tgcattgaca ctgatgcgg
//
LOCUS       DAWAAE010000127         5867 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5747_length_5867_cov_3.174295, whole genome shotgun sequence.
ACCESSION   DAWAAE010000127 DAWAAE010000000
VERSION     DAWAAE010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_5867_cov_3.174295"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1066..1075
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1214 a         1723 c         1781 g         1139 t
ORIGIN      
        1 ttatttgcgc ctgcgggcgc aaactctgcg aggctttttc gacaggctgt gcccccgctc
       61 ttcggatcgt tccgaaaagc gggggtcatt tgtataagag tcagccgttg agaccgcggg
      121 cctggcggtc catgtcctcg actacgatat cgaaaatttc gccgagcgag gcgcagtatt
      181 cgagcacctt ccaaggctca ttggtgtggt agtggatctt gatgagctca tcatcaccga
      241 cggcgagcag acagtcgccc tggaagttgg tgcggaaata atcgttgatg gcgtcctcat
      301 ccaggccggt gccttcgatg aggagctgcg tatcgtagcg gtaatcgatc atggaaatgt
      361 tcctttctgc aataagttac cggtgctcgc cgaggaagaa cagcgcaagc gtgccggggc
      421 cggagtgcgc gccgatcacc gggccgacga ccacgatctc cttgacggaa gcaccgtagc
      481 gctccttgag gatcgcggcg agcgtgtcgg cgtccgcgcg gcagtctccg tgagaaataa
      541 acaccgttcc gccggctttg tcaagagcga gctcgccgta tttgtcggcc atcgcgttga
      601 gagaggcttt gcggccgcgg accttgaaca tgttgatgag atgaccggcg tcgtccatgt
      661 gcagcacggg cttgaagccg agcgtgttgc cgacgaacgc ggtggcggcg ctgatgcgtc
      721 cgccgcgctt gagatagacg agatcgtcca ccgtgaacca gtggcagaga tggaagcggt
      781 tgtcgagcac gaccttttcg cactcctcaa ttgtgccgcc ctcgcgcttt ttctgcacgg
      841 ccatataaag cagcagaccg aacccgccgg acgcgccgag cgtatcaacg cagcggatgt
      901 cgcgctcggg atacttttcg cgcagctcct gcgcggcgat gcacccggcg tggtatgtgc
      961 cgctcagacc cgaggaaaaa ccgatgtaga ggatatcctt tccgtcctgc aggatcggct
     1021 caaagccctg caggaaggcg tccgcgttca cggcgctcgt gcgcgnnnnn nnnnncgcgc
     1081 atcgccgtcg aacacgtagg tcagccaaga gtacggcacg ccccactcct tcaaaagctc
     1141 cggcgggatg tcgcatgcgg aatcggtgta gatcacaaag tcgttcatgg tatcctctcc
     1201 ttatatatac agcaaaatat atacagcaaa ttatttcgat cggacgttct cgtccccgag
     1261 aaaaaacagc gccagcgcgc cggggccgac gtgcgagccg gtcacgggct cgtacatcac
     1321 ggtcatgatc cccttcggcg gcgcaacgat acgcagcgcc gaggcaaggt attccgcgtc
     1381 ctccgcgcag tcggcatggg caatgcctac ggtctgcgtc tcgggcgcga cagcatattt
     1441 gcggtaacgc tccacaagcg cgtcaatgga cttgcggcgg ccgcggatct tgtccacggc
     1501 gacgatcgcg ccctcctcgt tgcccttgag cacgggcttg atgtgcagca cggaggcgac
     1561 gatcgccttg gcgttggaga gccgtccggt gcggcgcagg tacatgatat cgtccacggt
     1621 gaagatctga tacatccgcc agcgcatatc gcgcagcgcc gccgccgttt cggaaagcga
     1681 agcgccgttc tcgcgcatct cgatggcgcg cagcacaaga atgccctcgc cgagcgaggc
     1741 gccgagcgta tcgatgagct ggatatcacg cccgggaaac tcctcgcgca gatcgcccgc
     1801 ggcgagcttg gccgagccga acgagccgct gatgccggac gacatgccga cgaagagaac
     1861 gtcttccccg gcggcgagca gaggccgcgc cgcggagatg aaccggtcgg ggtttacgag
     1921 cgaggtcgtc accaccgtgc ctttccgcat cgcgccgtag tacgccgcgc cgtcaaacga
     1981 cccggtatca agacaggtgt gctccgcacc gtcataggaa taggaaaacg gcgcaacggt
     2041 gatgccgtgc ttctgcagaa gcggcgtcgg cagattggcg gatgtatcgg taaggatagc
     2101 aaaagacaag atgtttgccc tccaagtatc taatatttga tattattata aatcaaggtc
     2161 aaaattaagt caatgggaaa agacacaatt gcttcttgta aaatcagcag gatgtggtat
     2221 aatcctgtct gcgaggtgat gaaaatggca tacgataacg cgctcgttgc gggcaagctg
     2281 cgccgctggg aaaagtatct ggacgatttc cggcttccgc gctgggagga gattcccaat
     2341 ctgggattgt atatggagca ggtcacggtg ctgctgcgcg aatatctgga ctatctcccg
     2401 ccggagctca aggatgagca gttcattacg gcggcgacga tcaacaacta cgtccgcatc
     2461 aaggtcatgc cggagccggt gaagcggcgg tattaccgcg tgcatatcgc gtatctcatc
     2521 atcattctga cgctcaagca gtcgctgagc atcgcgctca tccagaaggt cattccgatg
     2581 ggactttcgg aagaagaggt agagcatatc tacacggcct atgcctcccg ccaccggcag
     2641 gcggcgcagt acttcatcca gcagatccgg ctgacggcgg ggccgatcct caaccatgag
     2701 gagaacgcgg agatcacgac cgagcacgcc gaggatctta tcatgctctc ggccatcatg
     2761 ggcggcttta cgcgggtgtt cgccgaaaag ctccttctgc tcgacggaaa gaccctcttg
     2821 gacggcggca gcgtcgccca gtccggcgta aagcgggaaa aaaagaaaga taaagagtaa
     2881 aaaaatcctc cgatgaaaat cggaagattt ttttagtctt atggttcgcc gcagcctttt
     2941 caaacgaagc gtaaacgaag cgggtctcgt ttgagagcgg ttaccggatg gtctgcacct
     3001 tcaccatgtt ggtgctgccg tgggtgttga gcgggatgcc ggcggtcata acaacggtgt
     3061 cgccggagtg cagatagccg agctccttcg ccttttcaat ggcgcagtgg aacaggacgt
     3121 ccacgctgtc taccggctgc gtgcgcagcg ggtacacgcc ccagacgagc gcgagctggt
     3181 ggtagacctt ctcggacggc gtcgcagcga ggatcatctg cgccgggcgg tatttggcga
     3241 cgcggcgcgc agccttgccg gtgctcgtgt cggcgatgat cgcctgcgca tacgtctgct
     3301 cggccatcgt gcaggcggcg gcgctcacgg cgttggtgtt gtcggagtga tccatcgcgt
     3361 gccagatgtt cttgtagata tcggcggcga aagcgtcctg ctcggcctgc tcagcgatac
     3421 gcgccatagt cttcacggcg tccacggggt acttgcccgc ggcagtctcg ccggagagca
     3481 tcacggcgct gctgccgtca aagacggcgt tcgctacgtc ggaaatttcc gcacgggtcg
     3541 ggcggcggtt ttcttccatg ctgtcgagca tctgcgtggc ggtgataacg gttttacccg
     3601 cctggcagca ggaacggatg aagtgcttct ggatgccggg gagacgttca aacgggatct
     3661 ccacgcccat gtcgccacgg gcgaccatga taccgtcgga agcggcgagg atctcgtcaa
     3721 acttctcaac gccctcggtg ttctcgatct ttgcgatgat gcggatgccc tcgccgccgt
     3781 gggagttgag gaacgagcgc atatcaatga tgtcctgcgc gcggcgcgtg aacgaagccg
     3841 cgacgtaatc gacgttctgc tcgatgccga agagaagatc gctcttgtcg cgctcggaga
     3901 ggaacggcat gttgagccgc gcaccgggga tgttcacacc cttctggctc ttcatcatgc
     3961 cgccgtccac aacggtgcag cggatctcgg tgtcggttgt ctcctccacg cggaaggaga
     4021 gatcgccgtc gtccacgaga agcatatcgc cgctcttcac atcggcgggc agctcggcat
     4081 agttcacgaa gcacttttcg gcggtgccgg gcgtttccac ggtggagaag gtgtacgcct
     4141 gaccggaaac gacctcgacc ggctcatcaa agagaccgag acggatctcc gggcccttgg
     4201 tgtcgagcat tacggcggcg gggagcttca gctcatcgcg caccttgcgg aagcgcgcga
     4261 tcatttcggc gtggctctcg tgggtgccgt gggagaagtt catgcgggcg acgttcagcc
     4321 cggcgagcag gagctcgcgc atgacctctt cgctgcagct ggcgggaccg atggtgcaaa
     4381 tgatctttgt tctgcgcata tatccctcat ccttttctat aaatttctct atttccaagc
     4441 gcataatacc acaacctccg tcatatggca acgcacattc tacatagttt ttccacggca
     4501 aaccatacaa ttttcagcat gccccgcggc gcaggtttta tttgcattct gtataaactc
     4561 cttgcttttt cgccggaatt tgatataata cgaagctgga tacggaaacg tttcaaagtt
     4621 aacaacgggc cggtgccggt tctccggctg cggctctttt aggaaaggat ggatacacaa
     4681 tgatctactc agcggaagtc cagcatatgt gccccgttgc caagggcgca taccacggtc
     4741 ctgcgccgat ccccgaagag ggcaagtggg tgcaggcgaa ggaaatcaag gacatcagcg
     4801 gccttaccca cggcatcggc tggtgcgcgc cgcagcaggg cgcctgcaag ctgacgctca
     4861 acatcaagga cggcatcatt gaggaagcgc tggtggagac catcggctgc tccggcatga
     4921 cccactccgc agccatggcg agcgagatcc tgatcggcaa gaccattctc gaagcgctga
     4981 acaccgacct cgtgtgcgac gcgatcaaca ccgccatgcg cgagctgttc ctccagatcg
     5041 tctacggccg cagccagacc gcgttctccg agggcggcct gcccatcggc gccggtctgg
     5101 aagatctcgg caagggcctc acgagccagg tcggcaccat gtacgccacc aaggtgaagg
     5161 gcccgcgcta tctgcagctc accgagggct acgtcacccg tctcgctctc gacgaagagg
     5221 atcagatcat cggctacgaa ttcgtcaaga ccggccccat gatggaaaac atcaagaagg
     5281 gcatggacgc caacgaagcc gtgaagaagg ccaccggcag ctacggccgc tttgccgatg
     5341 ccgccaagta catcgacccg cgtcacgaat aagagaggag gacggaaata atggcactgt
     5401 ttgaaagcta cgaaagaaga gtcgacaaga tcaacgccgt cctcgccgag tacggcatca
     5461 actccattga ggagtgccgc gctatctgca aggaaaaggg ctttgatccc tacgagatcg
     5521 tcaagggcgt gcagcccatc gccttcgaga acgccggctg ggcgtactgc gtcggcgcgg
     5581 cgatcgccat ccgcaagggc gtgaagaccg ccgccgaagc tgccaaggcc atcggcatcg
     5641 gcctgcagag cttctgcatc cccggctccg tcgccgacga ccgcaaggtc ggcctcggcc
     5701 acggcaatct cggcgcgatg ctgctctccg acgatacgaa gtgctttgcc ttcctcgccg
     5761 gccacgagtc ctttgccgcc gccgaaggtg cgatcggcat cgtccgcaac gcgaacaagg
     5821 cccgcaagga gcctctgcgc gtcatcctca acggtctcgg caaggac
//
LOCUS       DAWAAE010000128         5830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5778_length_5830_cov_3.227532, whole genome shotgun sequence.
ACCESSION   DAWAAE010000128 DAWAAE010000000
VERSION     DAWAAE010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_5830_cov_3.227532"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1305 a         1672 c         1616 g         1237 t
ORIGIN      
        1 cgaacatgat catgacgaac gggccaaagc agcttttgaa gtcggagaaa tccagcttgg
       61 taaacccgga gagcatggag aatccgacaa agatcagagc aacgcccgtg acggaggtgg
      121 ggatcatgag gaaaaggggc gagaggaaaa cgcagacgaa gaacagaatg ctcgtgacaa
      181 ccgccgtaag accggtacgg ccgcccgcct ccacgccgat ggtggattca atgaacgtgg
      241 agatcgtcgt gcagccggtc gccgagccga cgaccgtgcc gacggcatcg acgaggaacg
      301 gcttttcaat gtgggggaag tttccttcct cgtcaagcat attcgtctgc gctcccacgg
      361 cgatcaaagc gccgaaggtg gcgaagaagt cgctggtgaa cagaatgaag atccagacga
      421 gagcgccggg aatgtctgcg agaagggatt tgaaatcata gcacaggaag aggttcccga
      481 gctcgctcac atccggcaga gagaaaaccg acgccggcag cgtggaaacg cccatgggaa
      541 tgctgatgat cgtgccgacg acaatgccga tgaggatggc gccgcggacc ttgtacgccg
      601 taagaacgcc catgacgacc atagtgatca gcgcaaggag gaccgtaggc tgagagaaat
      661 ctccgagcgc cagaccgttt gaaaaaacag ccagaccggt gttcttaaag ccgaggtacg
      721 caatgaagaa gccgaccgcc gtcggaatgg cgatcttcag attcttcggc agaagatcga
      781 caaccatttt ccgaacgccg agcaccgtca ccagaataaa cagtatgccc gagcagagaa
      841 gaagcgccat ggcccagccg aaggaaaacg cgccgctgtt tacgagaccg ccgagaatga
      901 agttgctgcc catcgccgtg gaaagggcaa gcggaagatt tgcataaagc cccataaaga
      961 gggtaatcgc tccgctgacg agcgcgcaca tgacaagcag cgcagatttg ctgatgacaa
     1021 ggccgttcac gtctgtgaac accggatcgg ggccgaagcc gcagatggcc gccggctgca
     1081 ccgccagcac ataggccatg gtcataaacg tcgtgatgcc cgcaagcacc tcgcgcttcg
     1141 ttgtggtgcc gtgttcctga agtctgaaaa agtctttttt catgttttct cccttttatt
     1201 cgctggtttt ttctgtttct cttactccac gacctcgagg atcggatcaa cgacctgctg
     1261 ctttaccacg tcgataaagc cgcagtcggt aatggcgtat gcgggaatcg ccgcaaggca
     1321 gatgaagctc aggaacatga acggaatagc gatctctgtt cccatttcat gggcaacgcg
     1381 gttcagctcc tttttcttat ccgccagctc gtcgagcgaa agatcgctca gcaggccgca
     1441 gacgggatac gcgacttccg ccgcgatctc gccgttgcgg acgatgacct ggccgccgcc
     1501 gagctcggcg cagcggttta ccgccagggc catatcctca aagctgtcgc ccaggacgat
     1561 gatgttgtgg ttatcgtggc cgacggagga ggccattgcg ccgccgcgga tgcggaatcc
     1621 gcccatgaac gccttgccga tgttgccgtt ttttccgtgg cgctcgacct gcgcaatata
     1681 cagcacgtcc tgctccacgt cgcactgcac cacaccgtcc acgaccggaa gctccacgtc
     1741 gcggcggctc gtaaagggga tgtaggacag cgtgtccatt acaagcgcct tgaccttctt
     1801 tgccttggcc gacacgcggg tcttgaagct gtcagcggta accggctggc tcaggtgaac
     1861 ggtattcagc acgcagggat cgtgctgcgc ggtttcataa tgcacgagca gcttgccgtt
     1921 atcggtgatc tggcggccgc cggcaaaggt ggtcagcacg cggaaatctt ccgttccggt
     1981 ggtgatgttg atatccgcgc ggcggccggg agcgagacct ccgatctcac ggtcaagatt
     2041 gaacgcgcgg gcacagttga ccgtgaccat ctgaatggct tttacgaaat ccagccccat
     2101 gccgagcgcc gtgcggagcg catcgtccag atggccgcgc tgctgcagat caacggtatg
     2161 aaggtcgtcg gtgacaatgc tgaccatggc ggtgtccaga tgattttcca tcacggtctt
     2221 gagaagcacc ggcatgttgc gggcggcgct gccctcgcgc atcatgagat ggcagccgtt
     2281 gcgaagacgc tcaaaaacgt ctttctcgca gaacccttca tggtcggtgg aaacgccggc
     2341 cgccacgcat gcgctcatgg ccgggccgta catatccgga agatggccct gcagcgtttt
     2401 tccgggcatg gagagcgtgt cgtcacacga ctcgagcagg tcggggaagc ccgccataat
     2461 atagggaccg acacattccg acaggccgac ggcgtccgga cggcgaagcg cgcggcggat
     2521 gatctccgga ttgaagcggc ctccgctcgt ttcgagattg ggggaaaacg ggacatggga
     2581 aggaacaaca aaatagagct tgaggtcggt cgtttccgct tccttcagga tcgcttcgat
     2641 gccttccaga ccggaaacaa cgcctacctc gtggaggtcg gtcatgatgc ccgtggtgcc
     2701 gtgagagaga accgcttccg caaaggaccg aattgccaga gaggagcttt ccggatggat
     2761 atgtccgtcc aggaatcccg gagtcaggta ctttccttcc gcatccacaa cgcgggtgtt
     2821 ctcgccaacg cagtaatcaa catcgccgac ggccgcgatc gtatcgccgc agacggccac
     2881 gccgccctcg tagatctccc cggagtaaac gttgacgatc tttccgttcc gaatgacgag
     2941 atctgccggg gtttcgcctt tggcgacaga cagtagtttc agattcatgc tgtacctcca
     3001 aaattttttg tattttgttg acccgtttta tttccggcgg cacggtgtac cgcagaaaac
     3061 cagtttagac ttttacgacc ggaaaacggt ccggcagaac gatgcccgca cgcttataaa
     3121 tggctgcggc ccgttcacaa gcctctttca aaattgcctc ctcatcaagc gtcaggattt
     3181 ttctttcacg catgagccac ttgccgtcgc acatcgtgga ttcgatattg gccgaatgca
     3241 ttgccgtgac cagcgcggca attttatcgt tgaccggcat catggacggt ccgtgcggat
     3301 caataacgat caaatcggcc ttttttccgg cttccagact gccatacaga ttttcatcga
     3361 gaagcgcacg cgcgccccac tttgtcgcca tgcgcaggat atcctgtgac gggaccaccg
     3421 tcggatcgag ccgccacccc ttatgaataa ggctggtgag ccacatttca tccaccatgt
     3481 ccatgtggtt ggaggacgaa gcgccgtctg tgccgatgga cggcatcagt cccatctgca
     3541 gcattttcgg aacgcgggca aaacccagca cccgcatggc cgacgcggga ttgtgcgaga
     3601 tcttcacttc ccgcttccgg aagagctcca gctcttcatc ggtcagccag accgtgtgta
     3661 ctgccagaag atttttatcc agcacaccga gccgttcgag gtgctttacc gtcccctcgc
     3721 cccagcgcgc ataggtgtat tccttttctt cctttgcttc cgccacgtgc atgtgaatgc
     3781 cgacgccgta cttgtcggca agctcttttg tgcggacgca aagctcatcg gtgttgttga
     3841 atatcgtccg cagaccaaac catacctgca cgcgcccgtc agcggtattg tgatattttt
     3901 cgatatccgc gacctgctgc tcaagctcct gctccatcgt gcgctgccag atcggcggga
     3961 ggccttcacc gcagtccatc accgacttgg cgagctttcc gcgcaggccg gactgctccg
     4021 tggcccgcgc catgccggag acgaactgac cgcccggctc ggcaaacgac gttacgcccg
     4081 agcggatcag ctccaggctc gtcatgagtg tggaaacgta ggaatcctcc tccgtcatat
     4141 tgctctcaaa gggccacatt ctctcgtgca gccagcagat aaaatccacg tcgtcgccga
     4201 cgccgcggga tatctgctgc gaggtgtgga cgtgggtatt cacaaatccg ggcagaacat
     4261 attttccgcg caggtcgatg acctccgcgt cgggcgagat cttcgccgga tcgacccggc
     4321 cgaccgccgt gatgcggtcg ttttccacga gcaccccgcc gccgtcaaac acggtgtcgt
     4381 tttgattcat ggagacgata tagccgttct tcaaaaaagt ttgcatgttc ttcctccatt
     4441 aacttgattc gatcgcagaa aaatgatatg ctcacgctac aacctataat ggttttaggt
     4501 tcaagtcttt ttttctgttt ccggagcttt tctgatgttc ctacaaaacc aatcgacagt
     4561 gccggagaaa acttgggagg ttttatgaaa gaatttttgt cgatcggcga gcttgcaaaa
     4621 attttcgaca tggatgtgca gctcctccgc cactacgacg caaaagggct tcttgtcccg
     4681 caggtacgca acagcgaaaa cagccgccgg ttttatcact ttgaccaggt ctatccgctc
     4741 gccaccatac gatatctgcg ccgccttgga tacccgctcg cccagatacg ggattttctc
     4801 cataacaacg cgctgcggga caatcttgaa atgctctctg agcaggcgga aaaacttcgc
     4861 cgccaatgcg acgagctgaa cgctaccatt cagatcatcc agcaaaaagt agaatttatc
     4921 gaccgggagc aatcctccag ccagagagga aagttttccg tccggacgtt tccgcgccgc
     4981 gcctttctgc acattggcga tgagatcaat ctcttcacgc atgagctttt ttatttttat
     5041 cctaccgtcg gcttttaccg cggtgagcgc aaatggttcg gcgctctgct ttacgaagac
     5101 acccccgtgc aggaataccg tttccccgag ctgatggcgg agcaggctct ctcctatatt
     5161 cctgcgggcg agtatctctg cggctatcat tacggaccgt atctctcgat tcaggactcc
     5221 attgatcgtc ttttcgcgga agcgagccgc cgggagctgc gcgtggatga ctgcgtgatc
     5281 acgccgaata tcgtcgatca atgctgtgaa ggccatccgg aaaactacat taccgggctg
     5341 gaagtccgta ttttgccgga agagggcata gcggcggaag agcatctgat gcccggccct
     5401 gtcggataaa aaaacggccg gaggcagtat cacaaactgc ctccggccat ttttgcaaat
     5461 tgattcacac aaacacaagc tgcacgagca gcatgtaaac gacggttccg gcgacgatgc
     5521 tcaaaagcgt attccgacgc gcagatcaaa gcgctgctga aagtcgtcaa taaggaaaag
     5581 ggcggctgga agaaatttgt cgaagagacc agacggaaga aataaccact ccgtatatag
     5641 gcaaagccgg aagcccacgg gcttccggct ttgcctattc cttttattcc ttatccttat
     5701 ccttttgcag cgcggcaagg tctttcagca ccgccagagc gattggcttc acctccggcg
     5761 tgcaggactg aaacagctgc agcaccgttt cgctgccgta ggtcttctgg ctgtactgac
     5821 agccggacaa
//
LOCUS       DAWAAE010000129         5768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5853_length_5768_cov_2.862944, whole genome shotgun sequence.
ACCESSION   DAWAAE010000129 DAWAAE010000000
VERSION     DAWAAE010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5853_length_5768_cov_2.862944"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2611..2620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3452..3461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1105 a         1734 c         1776 g         1133 t
ORIGIN      
        1 tcatagaaca gcggctcggc tcgctggctg ccgcgctgga gtaactacat attaataaaa
       61 agtaatcatc gtctccgaag agccttttct tcggagacgg tttcttttcc ttgacggaat
      121 cgccggagga atctataatc agagtgttaa aatactttgg cgggatatcc cgccggggag
      181 gtacgaatac tttgacgcag gacgaaaaga aacagctgaa gatcgccgcc tgcaaagtgc
      241 gcctcggcat catcgagggc gtttacgcgg caaaatccgg gcatccgggc ggaagccttt
      301 ccgccgccga tgtgctcacc tatctctatt tcaagcacat gaatatcgat ccgcaaaatc
      361 cgcgctggga aaagcgcgac cgctttgtgc tctccaaggg tcatgcggct ccggcgctct
      421 actcggtgct cgccaaccgc ggctttttcc ccgtggacga gctcaagacg ctccgccata
      481 tcggcagccg cctgcagggc catcccaaca tgaatctcac gcccggtgtg gacatgtcca
      541 ccggctctct cggccagggc gtatccacgg cggcgggcat ggcgctcggc gcgaagcgtc
      601 ttggaaacgg cgtgcgtgta tatacgctcc tcggcgacgg cgagatcgag gaaggccagg
      661 tctgggaagc gatgatgttc gcctcgcact ataagctgga taacctgctc gtgttcgttg
      721 acaacaacaa tcttcagatc gacggcgccg tgagcgacgt tatgagcccc tatccgatcg
      781 acgagaagtt cgccgctttc ggactctttg tgcagaccgt ggacggccac gactttgacg
      841 cgatcgacgc ggccgtgaag cgcgccgagc aggaaaaggg acgcccctcc gcgatcgttc
      901 tcaagaccgt taagggcaag ggcgtctcct ttatggaaaa ccaggtgggc tggcacggca
      961 aagcccccaa cgaggaacag taccgcaccg cgatggaaga gctcaccgcg gcgctgcgcg
     1021 aagaggaggc agaatgatgg cggacgttgt aaaaattgct acccgcgaca gctacggcaa
     1081 cgcggtaacg gcgcttgccg ccgagcatga cgatatcctt gttctggacg ccgaccttgc
     1141 cggcgctacc aaaacggcga tcttcaagaa ggcctatccc gaccgccaca tcaactgcgg
     1201 cattgccgag gcggacctca tcggcgtgag cgcgggcctt tccacgatgg gctttgtgcc
     1261 gttctgctcg tcgttcgcaa tgtttgcggc gggccgcgcc tatgagcagg tgcgcaacac
     1321 cgtcggctac ccgcatctga acgtcaagat ctgcgccacc cacggcggca tctccgtcgg
     1381 cgaggacggc gcttcccacc agtgctgcga agacttcgcg ctcatgcgca cgatccccgg
     1441 catggtcgtg ctctccccgg cggacgatgt ggaagcgcgc gccgccgtca aagcggccta
     1501 tgagcacaag ggtccggtgt acatccgctt cggccgcgcc gccgtgcccg tgatccacga
     1561 ggaggagaac tatttcttcg agatcggcaa ggcggaggtg ctccgccccg gcacggatat
     1621 cgccgttgcc gcgaccggtc tcatggtcgc cgaggcgctc aaggcggcgg aaacgctcgc
     1681 cgcggagggc atcaatgtgc gcgtcatcaa cgtctgctcc atcaagccgc tcgacgagga
     1741 gaccatcctt gccgccgcgc gcgagtgcgg gaagatcgtc acctgtgagg agcacagcgt
     1801 catcggcggt ctcggcgagg ccgtgtgctc tctcgtaagc gagaagcatc ccgtgccggt
     1861 gcgccgcgtc ggcgtgaacg atgagttcgg ccattccggc ccggcggcgg cgctgctcga
     1921 agagatgggg ctttgcgctt cgcacatcgc cgaagttgtc cgcgagaccg ttaagggctg
     1981 ataataaaaa gggggataca ccatgcgctg cagaacgatc atcaaattcc gcaacagcga
     2041 cgatccgaac gatctcggct tcggccgggg catcgttcag cttctcgaag gcgtggagga
     2101 gcttggctcc atcaaccgcg ccaccgcctc catgggcatg gcgtattcca aggcatggaa
     2161 gatcatcaat gccatcgaaa aggaatttga cgtccgcctc atcgaccgcg agggcgcgca
     2221 cggctcacac ctgacggacg aggcgcgtgc gctgatccgg cagtatcacg aggcgcttga
     2281 cgccgccgac gccgcggcgc aggcggtatt ccgcaaatac tatccctgaa aaggcttttc
     2341 tgccccggta cggatcacaa accgtaccgg ggcattttta tttcgttgac agagacgtgt
     2401 gcggcgtggt actattctct ggtgaaaata catgaaaggg agggacgctg ttacggcgga
     2461 taaaacatcg gtctatgccc ggcggttccg ggtgcttgcg gcggcgtttc tgctgctgtt
     2521 tttcggttcg ttcctgctgg ggcggtttgc cgttgcgccg ggaacgcttt tccgcatttt
     2581 gtgggacgcg ctgcgcgaat gggtgtgtaa nnnnnnnnnn agctcttcgg cgcggaagtg
     2641 ccgggggaac tgtattccgg ccaggaaaag gcggtcgtac tgaacatccg tctgccgcgc
     2701 gtggcgctct cggcgctggt cggcgcggga ctttccgttg cgggtacggc atatcagggc
     2761 atgttccgaa acccgatggt atcgccggac attctcggcg catccaacgg cgcgggcttc
     2821 ggcgcggcgc tcggcattct tttgtcgttc aactatttcg gcgtgacggt gagcgcgttc
     2881 gtctgcggcg tgggcgcggt ggcgctcgcg tggagcatca gccgggcaag ccgcatgaac
     2941 ggaacacttg ccatggtgct tgctggcatc gttgtcggct cgctgttctc ggcgggcaca
     3001 tcgtttataa agctcgtcgc ggacaccgaa cagcagctcc ccgccatcac atactggctg
     3061 atgggctcgc tctcttcggc caaaacgcgc gatctgcagt ttgctctcat cccggcgctc
     3121 atcggcatgg tgccgctctt tttgctgcgc tggcgcatca acctgctcac gctcggcgag
     3181 gaggaagcgc gcagtctcgg cgtgcatacg acgcggctcc gtcttgtgat cgttgtgtgc
     3241 gctacgctta tcacgtccgc ctgcgtttcc gtgagcggta tgatcgggtg ggtcggcctt
     3301 gtcatcccgc actttgcgcg gatgcttttc ggacatgatt ataagcgcct catcccggcg
     3361 tccatgctgc ttggcggggc gtttcttatg gtcgtggaca atatcgcccg tctcgccacg
     3421 tcgggagaga tcccgctcgg cattttgacg tnnnnnnnnn nttgacgtcg gtcgttggcg
     3481 cgccggtatt cgtctggctc attttgcggg gaggtgcgga ccgtgagcat tgagatccgc
     3541 cgtctcggct tttcctacgg cagcacggac gtgctgcatg atatcacctt caccgccgcc
     3601 aacggcgaca ttgccgccgt actcgggccg aacggcgtcg gaaagagcac gcttttccgc
     3661 tgcgtgctcg gctttctggc gccgtcggag ggcggtatcc ttgtaaactg caaggacatg
     3721 cgcacacttg atcgccgtgc cgcggcgcgc gagatcgcct atatcccgca gtcgtgcgtc
     3781 ccgtccttta actatacgct gctcgaagtc gttatgatgg gacttaccaa ccggatcggc
     3841 gttttcgacg cgccccgccc ggagcataag accgcggcgc tcgaagccat ggaaagtctc
     3901 ggcatcgcgc atctggcgca ccgggaatcc ggccgcgtga gcggcggcga gcggcagctc
     3961 gcgctgctgg cgcgcgccat ggtgcagaac gcccgcgtcc tcgttatgga cgaaccgaca
     4021 gccaatcttg actgcggcaa cagcgcgctc gtaatggagc gcgtgagcgc tctggccggg
     4081 gaggggttta cggtgctctt ctccacgcac gaccccaatc aggcgttccg gtatgctacg
     4141 caggtgctcg ccctccaggg cggcggcgta ctcgccgcgg gacggccgga ggaagcgctc
     4201 acaagcgaaa cgctcacaaa gctctacggc gtcggcatgg cggtatgtcc cgtgacggct
     4261 gccgggaaga gcttctccgt cgccgtcgtg accggcggaa aaacgcaata aggttgaaat
     4321 tatccggaaa gtaggctaaa atgctttccg gatcatttta tagataagga gcttttttat
     4381 ggaagccttg aaatttcgcg aaatgcccta cgagcgtccc gacggcgcgg cgctgaaaac
     4441 ctccatgcgc actctgacgg aaaagctgcg cgccgcgggg agctatgatg cggcgaaggc
     4501 cgtgtttctt gaaaaggaaa cgctctccaa acacatccag acgcttgcaa cgctcgcgca
     4561 ggtgcgccac acgatcgaca cgcgcgacaa gttttatgac gaggagaacg gcttctggaa
     4621 ccagctttcc ccggaactgg aggagtattc gcaggagtgg acgaaggcga tgcttgcctc
     4681 gccgtaccgc gcggaatttg aaaaggaata cggcacgctg atgttcctca acgccgagat
     4741 cgctctcaaa acgttctcgc cggagatcat cccggagctg cagaaggaaa acgatcttac
     4801 gaccgaatac gataagctca tcgcctcggc gcagatcccg tttgagggcg gtgtatacac
     4861 gctctcgcag ctctcgccgt tcaagaccgg cgcggacgac gcgcagcgcc tcgccgcgtg
     4921 gaaggccgag ggcggatggt ataaggagca ccagagcgag cttgaccgca tttacgatga
     4981 gcttgtccac ctgcgtgaca cgatgggcaa aaagctcggc tacaagggct atacggagct
     5041 cggctactac cgcatgggcc gcaactgcta cggcaaggcc gatgtggaaa agttccgcgc
     5101 cgccgtgcgc aagtatcttg tgccggtcgc cgagagcatc tatcaggaac aggccaagcg
     5161 tctcggcaaa acgtatccgc tgagctttgc ggacgcggcg ctctccttcc gcagcggcaa
     5221 cccgcgtccc tgcggcgacg ccgagcacat tctcgctcac ggccagaaat tctatgatga
     5281 gctctctccg gagacgagcg cgttcttccg catgatgaga gaacgcgagc tgctcgatat
     5341 cctctccacc gagggcaagg ccgccggcgg ctattgctgc acgattcccg attatgacgt
     5401 cccgttcatc tttgccaatt tcaacggcac gcagcacgat gttgaggtcg tgacacacga
     5461 ggccggccac gcgttcgagg catatctcaa tgcatggcgc gtccccatgg acgatccgag
     5521 catggaagcg tgcgaagtcc attccatgtc catggagttc atggcgtggc cgtgggcgga
     5581 gggcttcttc ggcaaggata cccgcaagta tctttacagc catctcgcca aagcgctcac
     5641 gttcatcccc tacggcacga tggtcgatca cttccagcac gtcgtttacg aaaaaccgga
     5701 catgaccccg gcggagcgcc acggcgtctg gaaggagctg ctcggcgtat atatgccgtg
     5761 gatgaagc
//
LOCUS       DAWAAE010000130         5725 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5901_length_5725_cov_2.664198, whole genome shotgun sequence.
ACCESSION   DAWAAE010000130 DAWAAE010000000
VERSION     DAWAAE010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5901_length_5725_cov_2.664198"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4119..4128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1270 a         1697 c         1558 g         1190 t
ORIGIN      
        1 gtcatcaagg agcacccggt tctgctcaac cgcgcgccga cgctgcaccg tctgggcatt
       61 caggcattcg agccggtgct cgttgagggc cgcgctctca agctgcaccc gctggcctgc
      121 accgcctaca acgctgactt tgacggcgac cagatggcga tccacgttcc tctgagtgct
      181 gaggcgcagg cggaagcccg cattctcatg ctctcggtca acaacctcct gcgcccgcag
      241 gacggccacc cgcactccat cccgacgcag gacatggtcc tcggctctta ctacctcacc
      301 tatgtgcgcg aggaagaggg caccggcatg gcgttcagca cccccgagga agcgctcatg
      361 gcctatcagg cgggcgccgt cacgctccac agccccatca aggtccgcgt gaccaagacc
      421 atcgacggcg tggaaaagta caagaccgtc gaaacgaccg tcggccgcat catcttcaac
      481 gagcccatcc cgcaggatct cggctttgtg gatcgtaccg atcccgagca tgcgttcgat
      541 tacgaggtcg gcttccgcgt cgataagaaa aagctcggcg aaatcatcga ccgctgcatc
      601 cagaagcacg gcttcaccat ttccgccgaa gtgctcgaca gcatcaaggc gcagggctac
      661 aagttctcca cccgcagcgg catgaccatc tccatcgcgg acatggtcgt ccccgaggcg
      721 aaaaaggaaa agatcgccga gaccgagaag aaggtcctcg cgatcgaaaa gcagtacagg
      781 cgcggtctta tcaccaacga cgaacgctac cgcatggtcg tcgccgagtg ggaaagaacg
      841 accggcgaag tcgccaaggc cctgatggac aacctcgacg agttcaaccc catcaacatc
      901 atggcgacgt ccggcgcccg aggcagcgcc aaccagatcc gtcagctcgc cggtatgcgc
      961 ggcctgatgg cgaacaccgc cggtaagacc atcgagatcc ccatcaaggc gaacttccgt
     1021 gaaggtctga gcgttctcga gtacttcatc tctacccgcg gcgcccgaaa gggcatggcc
     1081 gatacggcgc ttcgtaccgc tgactccggt tatctgaccc gccgaatggt cgatgtcagc
     1141 caggacgtca tcatccgcga gccggactgc ggcacgaccg agggcgtgcc cgccacggcg
     1201 atcgacagcc gcggctacgc cgtgtttacg ctcgatcccg aggatccgaa gtacgacgag
     1261 tatatccact ccttcggtga gaacatccac ggccgcttcc cggccaagcc cgtcgtcgat
     1321 cccaagaccg gcgaggtgct gttcgatacc gatcacctgc tcatgtccgg cgatgcaaaa
     1381 ttcctcgccg ctcacggcgt tgcgagcgtt gaggttcgca gcgttctctc ctgcgaatcg
     1441 cgctgcggcg tatgcgcccg ctgctacggc atgaaccttg ccaccggcaa gccggtcaat
     1501 tccggcgaag ccgtcggcgt catcgccgcg cagtccatcg gcgagcccgg cacgcagctg
     1561 accatgcgta cgttccacac cggcggcgtc gccggtgacg acatcacgca gggtctgccg
     1621 cgagtcgaag agcttttcga ggcgcgcaag cccaagaaga tggccgtgct cagcgagatc
     1681 agtggcacgc tcacgatcga ggaagcccgc aagaacatga tggcgctcac cgtcaccaat
     1741 gccgagcagg gcgagacccg tgtgtatcag gtccccgttg gagccggcat catcgtgcag
     1801 aacggcgacc atatcgaaca gggacaggag ctgacccgcg gcgcgctcag cccgcacgat
     1861 gtgctccgca tccgcggcgt gaacgacgac gagttcggcc gcccgggcgt gcgcaactat
     1921 ctggtgcagg aagttcagaa ggtgtaccgt cagcagggcg tcgatatcaa caacaagcac
     1981 atcgaagtca ttgtccgcca gatgatgcgc aaggttcgca tcgaggatgc cggcagcacc
     2041 gatctgctct ccggctccac tgtcgacgtc aacgagctca aggacgccaa caaggcgatc
     2101 caggcgcgca tcgacgctgg ggaagaggga ctcactctcg ccgtcggcac gccgatcctg
     2161 ctcggcatca caaaggcgtc gctcgcaacc gacagctgga tgtccgccgc gtccttccag
     2221 gagacgacca aggtactcac cgaggccgcc atcaagggca aggtcgacca cctggtcggt
     2281 ctgaaagaaa acgtcatcat cggcaagctc atcccggccg gctccggtct ggacatgtac
     2341 cgcaactttg aaatgaagac ggacgagtcc atcgaggatg aagccgacta cgtcgacctc
     2401 tccgagctca aaaagctcac caacgcgctt taatcactgt ataagatcat aagatcagaa
     2461 aatgaggttg atgaattcaa cctcattttc tggttataga agaggggatt catatgagca
     2521 gggaaaaagt cccgctgcgt actctcggcg attgccgcag ctttcttatg ggcatcgcga
     2581 ttctcatgat tatattctgc catggatact acccgttttc cggactatac gggaaagttt
     2641 tcgattcggt cacgagtttt cttcaaagcg gagtggaggt attcctcttt ctttcgggat
     2701 atggatgctt ttattcttta agcaaaacgg aagtgaccgc taaaagcgta tttcggtttt
     2761 attcccgcag atttatccga attatccccg cgtggttttt cattgcgctt ttttccgccg
     2821 cgttaaaata ttttttcgcg ggagtagaat tttacgacgg actttcgcag ttctcgctgc
     2881 ttgcattttt tacaaaaggc agtctgggtt gctggtttat atccgtcatc ttggttttgt
     2941 atcttgtttc ccccttcttt tacgaacttt taaagcgttc tgccggaata tataaattgg
     3001 tttgcacact cattttcgtc gcgtcagccg ctgtaatcct gtttgtcaaa accgatccgg
     3061 tttttgatgt tgtaaaccgg atgctgatca atcggtcagc catgttctgg ttgggaatgt
     3121 atgctgccga taaagtgtct cgggatcaaa acgcggtttc tgcgctgtcc aagcggcagc
     3181 tttccgtgct cctgtgcttg ggcattctgc tctttgcggc agcaacgatt tttgaatgga
     3241 agttctatgc acggttactg ttcgtgccga tcgcatatgc tgcggctctt ctgctaagca
     3301 aaagcgcata taccggccat gcgcctgccc cttggttcaa actcctttcc aacctgggag
     3361 ctgttactct cgagtgctat ctactgaatg aaaacgtccg tgcctgcgtt tatacggtct
     3421 gtaaacattt tatttccaat cagatctttt tatcggtcat tgcaaatgca ggaatgctgc
     3481 ttgggaccgc agtccttgca tgggcgtttc atcaagcgct cggcaagctc acggcaggga
     3541 atcgaaccgg taagctgtat caaaaatgac ttgggatttt tacagaaaat ccttgactat
     3601 tcttgtgcaa tctgttagaa tcagaaaacg tgtgggcgca cgcccgcacg agagcatttt
     3661 tttgaggaaa ggaggaaaat catgcccaca tttaaccagc ttgtccgtaa gggaagagag
     3721 caggtgacct acaagtccac ctctcctgcc atgcagaaag gcctgaacac tctgaagaac
     3781 cgcgagaccg accagtcttc tccccagaag cgcggcgtct gcaccgctgt gcgtacctcg
     3841 actcctaaga agccgaactc cgctctgcgt aagatcgccc gtgtccgtct caccaacggt
     3901 tacgaagtga ccgcctacat tcccggtgtc ggccacaacc tgcaggagca ctccgtcgtt
     3961 atgattcgcg gcggccgtgt caaggacctg cccggtgtcc gttaccacat catccgcggc
     4021 actctcgatt cccagggcgt caatggccgc tgccagtccc gctccaagta cggcgccaag
     4081 agacccaagg cgggtaagaa gtaagcgaaa cccccaacnn nnnnnnnnga tatattttgt
     4141 ttatatgata accttttgga cacaacggcg gcagagcgct gccgtcgagt acctttgaga
     4201 tcatttcata atgaaggagg gaagcaaagt gcccagaaga ggtaacgttc ccaaaagaga
     4261 aatcttgccg gatcccgtat acaacagcgt tctggtgacc aagctcatca acggtgttat
     4321 gctggacggc aaaaaaggcg tgtcccagaa ggtggtttac gacgcctttg acctgatcca
     4381 gaagaagctg gatcgcaatc cgcttgaggt gtttaccgaa gctctcaaca acatcatgcc
     4441 ggctctggaa gtcaaggccc gccgtgtcgg cggcgccacc taccaggtgc ctatcgaggt
     4501 tcgtccggag cgtcgtcaga ccctcggtct ccgctggctg gtcggctact cccgcaagcg
     4561 cagcgaaaag accatgaagg agcgtctcgc caacgagatc atggacgccg tgaacaacac
     4621 cggcgcctcc gtcaagaagc gcgaggatac gcacaagatg gccgagtcca acaaggcctt
     4681 cgcccactat cgttggtaat ttcagcaaag gtagagattt atgcccagat catattcact
     4741 tgaaaaaacc agaaacatcg gcatcatggc gcacattgat gcgggtaaga cgacgactac
     4801 cgagcgtatt ctcttctata ccggcgtcaa ctacaaaatc ggcgagaccc atgaaggcaa
     4861 cgccacgatg gactggatgg tgcaggagca ggagcgcggt atcaccatca cgagcgctgc
     4921 taccacctgc ttctggaagg atacgcgcat caacatcatc gatacgccgg gccatgtcga
     4981 cttcaccgcc gaggtggagc gctgcctgcg cgtgctggac ggctgcgtga ccgtcctctg
     5041 cgccaagggc ggtgtcgagc cccagacgga aacggtgtgg cgtcaggcgg accattacaa
     5101 tgttccgcgc atgatctacg tcaataagat ggatatcacc ggcgccgact tctacaacgt
     5161 catggacatg atccatgacc gtctgaaggc gaatgccgtg cccatccagc tgcccatcgg
     5221 aaaggaatcc gacttccgtg ggatcatcga tctcatcgac atggattccg tcgtgttcta
     5281 cgacgatctg ggtaaggata tgcgcgtgga gcccatcccc gaggacatgg cggatcttgc
     5341 cgccgagtac agggaaaagc tcatcgatgc cgtatctctc tttgacgatg agattgcgga
     5401 gatgtatctg gaaggcgagg agatccccgc cgaccgtctg cgctcggtca tccgcaaggc
     5461 gaccgtcagt ggcagcatgg ttcccgtcct ctgcggtacg tcgtacaaga acaaaggcgt
     5521 gcagaagctg ctcgatgccg ttgtcgattt cctgccctct ccgctggatg ttccgcccgt
     5581 tgtcggcgtg aaccccaaga cggacgaaga ggtcactctc cccgcggacg tgaacggccc
     5641 ctttgccggt ctcgtcttca aggtgatgac ggacccgttc gtcggacgcc tggcgttcat
     5701 ccgtgtctac tccggctcca ttaag
//
LOCUS       DAWAAE010000131         5697 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5944_length_5697_cov_2.538639, whole genome shotgun sequence.
ACCESSION   DAWAAE010000131 DAWAAE010000000
VERSION     DAWAAE010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5944_length_5697_cov_2.538639"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4298..4307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1192 a         1638 c         1737 g         1120 t
ORIGIN      
        1 tgtgtgctct tgttaatcga cgctaatatg aacaggcaaa aactgcggat gctcaaaagc
       61 atcctcagtt tttgattttt attcttcgtc ggtctcgagg acgacctcgt ccgtgccggt
      121 aaccttcttc tcgaactttg ccataaggaa cggagagaag atgccgacaa tgctcagggc
      181 gatgagcacc cagcacaccg gggaggcgaa cagaacaccg atatcgccgc cgctgacgcg
      241 cagcgcaccg cgcaggccct tttcactgat tgggccgaga atcagtgcca gaacgaccgc
      301 cgcggtattc agatcgaatt tccgcatcag atagccgagt ccggcgaaga tcatcatgac
      361 gaccacgtcg ctcatgttgc ggtggatggc gtaagaaccg atgaagcaga gcaccgtgac
      421 gcaggggatc agaatggcgt cggacaggcg ggagatctgc gcaaagccgc ggcagccgag
      481 catgccgatg aacagcatga agaactgcac gagaacaaag cccgcgaaga acgtgtaggt
      541 catcgccgcg tgcttggtga agagctcggg gcccggggtg agaccctgaa tgataagacc
      601 gcccatcaga actgccgtga cggattcgcc ggggatgcca agcgtcagca tcgggatcag
      661 cgagccgccg gtaacggcgt tgttggccgc ttccgagccg cagacgcctt cgatggcgcc
      721 gtggccgaag tcctccttgt gcttggagaa ctgcttggcg ttattgtagc ccatgaagca
      781 ggcgatggaa gcgcccgcgc cggggagaat gccgatgatg ttgccgatga tccaggaacg
      841 gatgaccgtc ggcgcgagca tcttcttctc ggctttggta agggcgatct tgtcggagaa
      901 cttggaggcc ttctggcgct ccttgatctt cttctcggca aggatgagca cctgcgacat
      961 ggagaagagg cctatgagcg cgcacgtcgt gttgatgccc tcggcgagga acgcatggcc
     1021 gaacatgaag cgcttggtgc cgtcgatggg gtcaaggccg atcgtggaaa ggagcaggcc
     1081 gagcgcgccg gacatgagac ccttgacgat ggatttactc gaaacgccgg cgatgatcgt
     1141 aaggccgaag ataccgagcc agaacttttc cgccgacatg aatttcagcg cgagctgggc
     1201 aagcagcggg gcgaagaagt acagggagat gcagctgagc aggccgcccc agaaggagct
     1261 ccagcaggcc gtggcgagcg ccttgcccgc ctgacccttc agcgtcatct ggtacccctc
     1321 tatcgccgtt gccgcagacg ccggggtgcc gggcgtgtgg atgaggatgg cggagatgga
     1381 gccgccgaag atggcgccgc agtaaatggc gccgaggacg atgagtcccg tgtccgccgg
     1441 catggtaaac gtcagcggca gcatgagcgc aacgcccatc gccgccgaga ggccgggcag
     1501 acagccgatg atgatgccga gcgtgacgcc gcccaccatg gcaaggaggt tgatgagagt
     1561 gagagcctta ataaagccct gacccataag agccaatgtt tcaagcatag atcacatttc
     1621 ctccttaaaa cagaatgcct gcgggaagcc gcacgccaag gaagagaacg aacgcaagat
     1681 aaatgagaag atcgatcgat acgatcgaaa tgaccgtggc cagaatgggc acgcgcagga
     1741 agagaaggca agcgagcatg aacagcgttg tcgaaacgaa gaagccgacg tacttcataa
     1801 gaaagaagta cacgaggatc agcgcgaccg atacgatgaa ctgcaccggc ttgaagccgg
     1861 cgaaaacggt gtcaacaccg ctttctacgc cgaagcggcg ggcattcaat atcatctgga
     1921 cgagatagaa agtcgtgagg aagaacagaa gagcaatggt gaacttcggg tatgtacggc
     1981 tcgtgtcgga gagcttcgca ctgtaggcat agaagaagcc gcacaccgcg tacatgaata
     2041 ccacgactcc tatatcggtt ttttgcattt tcataagcag gagtcctccc gtgagaaatt
     2101 tctgcgggga ttatttacga taatttttta agaagcccgg agggggatac ctccgggctt
     2161 ttggtatcag cgaggattat tcgtaccaga aaccggtagc aaggctttcg gtgaactcct
     2221 gctgctgctc gatgagagcg gcggtggtgg cggcgtcctt ataatcggtg gcgaaggaag
     2281 cggcagccgc gagataatcg ggatcttcca tggtcgcctt gaaagcggcc tcgtagaacg
     2341 cgatgacttc cgggctcacg ccggcgggag cgacgacgca gcgggaggag ccgagccact
     2401 ggtcatagta gcctttctcg ccgagagtcg ggacgtcggg gaactcaaaa atacgctcgg
     2461 tggagaacac ggcgagcggc ttcacgtcgg tgcggccggc gatatcggcg gccttcgcca
     2521 cgcaccagtc ggcctctttg ccgagaaggc tggtgagctc gtccgcggta ttgccctgcg
     2581 gcaggtcgat gtacttgaag ccggcggagt tggcaaacgc ctgcgcgccg atgtggttgg
     2641 aggcctgcgc gccgttggtg gaggcgacgg tctggttttc cttggcgtaa gcgacgagat
     2701 cgtcaatgtc gttgaagcgc tcgtcgtccg cgcgcacgag gacgagagag gtctcggtca
     2761 catggttgca gatggcggcg aagtccgcgg tggtgtaggt gccgagtccc tttacgatgg
     2821 atgagttgaa gttcgggaga ttcatgaagc cgatggtcat gccgtcgggc tcggattcgg
     2881 cgagctgaga ccagccgatg gagccggagc cgccgtcaac gttctcgatg acgatggtct
     2941 ggccgacgta cttttcggcg tactgcgcaa ggatacgcgc ggtgacgtcg gtgccgttgc
     3001 cggctttgta ggcaacgatc atggtcacgg ggccgttggg ggcccagccg gtggtttccg
     3061 ccggagcttc ggcgggagtt tcggtctggg gttctgcggg agtctcgggc tgcgcgggag
     3121 cctcggtctg gccgcaggca cacagcgcga acatcataca gagggcaagg aacagggcaa
     3181 atgctttttt cattttttct ttcctttctt tcttttgatg gattcggtct cttcgttcct
     3241 cccaagagat gctgcaatgt taccagaatg ttccgttatt tgcaaggaaa attttacaaa
     3301 aaatccaaac agaagaaaaa catgttaaat ccctgtaaaa tctccgtaaa ttttctgttt
     3361 tccccggcaa aaaagcatcc ggacggcttt acaccgtccg gatgctattt acctataaaa
     3421 tctttgcagt ttcccaacac gcttttacgc cgcgccgaaa aagcggaaca cggcggtcac
     3481 atagaccaga agctggtcgc ggctgacgaa cttggtcata tcggcaatgt cggtcgtctg
     3541 gatcaggcgc tcgaagctgc cgccggtcgc atacgccacg gcgagcagcg caatgccgaa
     3601 gatcatcagc acggaaaaag cgatggctac ccatctccat accttactca tcggcgtctt
     3661 cccccttctt cggatacagg atcgcgcggt aaatgctgcg cgtgaggcgc accgtgagag
     3721 tgcagcccgc cacggcaatg gaaataccgg tccagaggag cagcagcgcc agcgcaaacg
     3781 cggccagcgt cgcgcctgcc gtgagcagca gatccggcat gcggacgatc gcgccgttga
     3841 acacataacc ggtcagatac acgcccgccg cgagaagcgc cgcgccgatg cccagaacgg
     3901 caatgccgag agcaagcgaa agtccgaaca tcagcaccca taccgccagc atcgggatcg
     3961 tgaccacgac cgcggcaaag gcgcgtccgc ccgacacggg gcggcgggtc tcgatctctt
     4021 cgtattcata cccttcttcc gcttccggct catcggcagg ctcttccgcc tcggccgctt
     4081 cttccgcttc ttccgggaag ctctcttcct cgggcaggat ctcggataca tcctccgcgc
     4141 cgtccggcgt ttccggctct tccggctctt ccggctcttc cggctccggc atctcgggct
     4201 ccggctccgc cggcgcttcg tccgcggtct cctccgctgc gacgtccgcc ggttctccgg
     4261 ccggctcttc cgcatccgga tgtgcagact tgtccgcnnn nnnnnnnggg tgattgacaa
     4321 tgcggtcgat tttgaagaca agctccgccg cggcatccgc gagcagtgcg tcgaggatct
     4381 cgtccgcaga ctcgatccgg cgcggctcgt cgtccggcag gacctcttcg ggatacagcg
     4441 gttcgagcgg cagtggctct tcgtcctttg ccggtccgtc cggcgtttcg cccgcttcct
     4501 cttccggctc ttcctccggc gcggcaggca tgaccggcgc agccggtttt ttcgtgaacg
     4561 tcaggccgag atccgaaaag tccagctcgt caagatcgat ctccggcagc acggcaggct
     4621 ccggctccgc ggcgatctct tcttccggct cgggctccgg cagctcttcc ggcagctctt
     4681 cgataagctc ttcggggaga tcttccggca gcggctcttc cgcttccggc tcgctctctt
     4741 cggcgcgtat atgtgcaccg gtgtattctt cctcgatgat ctcggtttct tccggcgctt
     4801 cggtctcctc cggcccggcg gcttcgccgt cggcaaacgg cgtttcgccg ttcgctaccg
     4861 cttcgcggta ttcccgctcg atctgcagca cctgacgtac cggcgagccg aagcagcgga
     4921 cgaccgtcgt ctcgccctcg ggaccggctt cgtcaaacat gcgctcatac cggttgagga
     4981 cacggtcgcg ctcggcagcg tcaaggaacg ccagcaggct ccgcagctcc gccatgaagc
     5041 tttttcggtt caacgtggga cacctcccat tcgtactgtt acaatttgtc atcgttagat
     5101 tatactattt aataaatact tgtcaagtct tatcccacga caaaccttga caagcgcgcc
     5161 gctctcacct aatataataa agttaaatcc gcggtgggag aagggctccc gccgttataa
     5221 aaggagaaat atcatggcga aagaaaaaat ggtcgagcag atcaccaaca tggagaccga
     5281 ttttgctcag tggtacaccg acatctgccg caaggccgag ctggtggagt atgcctcggt
     5341 aaagggcttc accatcctgc gtccctacgg ctacgcgatc tgggagaaca tccagcagat
     5401 catggacggc gagttcaaaa agaccggcca ccagaacgtg gcgatgcccg ttctgatccc
     5461 ggagagcctt ctgaaaaagg aaggcgagct cgtcaacggc ttcgcgcccg aggtggcatg
     5521 ggtcacgaag ggcggcagcg aggagctgga agagccgctc gcgttccgcc ccacgagcga
     5581 gacgatgttc tgcgaccact ggtcgcgcgt gctccactcc taccgtgagc tgccgatgct
     5641 ctataaccag tggtgctccg tcatccgctg ggagaagacg acccgtccgt tcctgcg
//
LOCUS       DAWAAE010000132         5651 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_5994_length_5651_cov_4.206397, whole genome shotgun sequence.
ACCESSION   DAWAAE010000132 DAWAAE010000000
VERSION     DAWAAE010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_5651_cov_4.206397"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          962 a         1775 c         1798 g         1116 t
ORIGIN      
        1 cgccgcgcag cagcgcgacg tggatgagcg cgttcaccgc gcccggcggg tcgccgaagc
       61 ggtcgatgag ctcgtcggtg aggtcgtcgg cgtccgcctc ggtgcggatg agggcgatgc
      121 ggcggtaaag gtccatgcgc tgctcggcgc tcgggacata cttttccggg atgttcgccg
      181 taacggagag gtcggcggcg cactcggtgc gcacgggcgg cttttcgccc ttttcctcga
      241 gcacggcctc ctcgagcagc tttaagtaca tatcgtagcc gacgtccatc atgtggcccg
      301 actgctccgc gccgaggagg ttgcccgcgc cgcgaatttc caggtcacgc atggcgatct
      361 ggaacccggc gttgaactcg gcaaactcgc ggatggcggt caggcgcttt tcggcgatct
      421 ccgtgagcgc cttctccggg cggtagagca gatacgcgct cgcgcgacgc gcgctgcgtc
      481 cgacgcgccc gcgcagctgg tggagctggg caagaccgag cttgtcggcg tcctccacga
      541 tgagggtgtt gacgttcggg atatcgatgc cggtctcgat gatcgtcgtg cagacgagca
      601 cctgaatttt cccctctacc atgtcgtcca tgacgcggga gagctgttcc tcgtccattt
      661 tgccgtgcgc cacgccgacg gaaacaccgc cgccgagctg cttttgcagc cgcagcgcca
      721 catgctcgat gttctccaca cggttgtgga gatagtatac ctgcccgccg cggagaagct
      781 cgcgccggat ggcgtcacac acggcctttt cgtcctgctc catcacgaac gtctgcacgg
      841 gctggcggtc gcgcggcggc tcctcaatgg tggacatgct gcgcagaccc gagagcgcca
      901 tattgagcgt gcgcgggatg ggcgttgccg agagcgtcag cacatcgacg cccttcgaca
      961 tttccttgag cttttccttg tgcgacacgc cgaagcgctg ctcctcgtcc acgatgagaa
     1021 ggccgagatc cttgaaggca acgctcttct gcagcaggct gtgcgtgccg atgagaagat
     1081 cgacgctgcc cgcgagcgtc tttttgagaa tgtccttttt ctggccgggc gtgctgtagc
     1141 gcgacaggag cgcgatctcc accgggtagc cgaaaaagcg gcgcatcgcg gtctggtaat
     1201 gctggcgggc aagcaccgtg gtcggcacaa ggatcgcgca ctgcttgccg tctaaaatgc
     1261 acttcatcgc cgcgcggagc gctacctccg ttttgccgta gccgacgtcg ccgcagagca
     1321 ggcggtccat cggaaccggc ttttccatgt cgtccttgat ctcctgaatg caccggagct
     1381 gatcgtccgt ttcggcgtag ccgaacgcct cttcaaactc cgtcatccac gggctgtccg
     1441 gcgcgaaggc gtagcccggc gtgcgggcgc gcgcggcata gagcttcgtg agctcggcgg
     1501 cgagctcttt tgccgcggcc ttggcgcggc tcgtcgtctt tttccactcc gcgccgccca
     1561 tgcgcgagag cttcacgggg cggtcctcgc cgccgccgat gtatttcgac acaaggtcga
     1621 gctgtgtcgc cggaacgtac aggctgtccg acccggcgta acatatttta atgtagtcct
     1681 tttcgatgcc gtccaccggc agcttgaaga taccggcaaa gcggccgatg ccgtggtgct
     1741 cgtgcaccac aagatcgccg accgagagat cggcgcagga gccgagcttc tcgcggtctg
     1801 ccatggcgcg gcggcgcttg cggcttcgca gcgcgcccgc ggcgatctgc gtatcggtca
     1861 gcacggcaag gtgcgcctcc ggcgcttcaa gacccgcgga gagcgcgccc accgcgaccg
     1921 tagcgcgtcc ctccggcggc agagcgccgt tcgggaagcc gcgcagcggc ggtatgccgt
     1981 ttcgctcgag catcgcggcg agtacgtccg ctcttctctc gtccgaggcg aacaccacga
     2041 cgcggaaatc gttttcaaga taccgctgca cgtcggcaag cgccgtttcc gcgctgccgc
     2101 cgtagctcgg aagctggcgg acgttcgtat cgagcaccga ccggggggag aggggatagc
     2161 gccccgcgct gaaggcgtcc gccatcacaa cagcgaagtc cgcgattttt tcgcacgcgt
     2221 ccttccacgg caggagataa tcgccggttt ccgcgaccgg gaacgcgcct tcgcgcagga
     2281 tcttcagatc gtccgcgaga gaatcggaaa tatccttcgc tttttccgcg cagcggttcg
     2341 gcgcgtcaat ataaacgatg gcgtccggcg cgatgaaatc gagcgccgtt gaatactcac
     2401 tgtataattc cgggagatac cggtcgctcc aggacagggg cgcgccggtg gaaataagct
     2461 ccgcgtcccg gcggagacgg cgcgcaagct ccatctgctc gcgccgctcg gcgcgcgcgg
     2521 cgcggctcag aagcgccttg gcgatgctcc cggcgcggct gggatcaacg agcgattcgc
     2581 acgcgggcag cacacggaag gattcgatat tctccgcgcg gcgctgcgtg tcggtgtcga
     2641 aaaagcccat ggagtcgatc tcatcgcccc agaactcggc acgcacgggg cgctccgcgc
     2701 cgggggaaaa gatgtcaaga ataccgccgc ggcgcgaaaa ctggccgggg ccctccacca
     2761 tgtcgctgcg gcggtagccg cacgcaagca ggcgcggctc gagcgcttcg gggccctcgc
     2821 cctcgccgag acggagcgta aaggcggcgt tttccagaat atccggcggc agcgtgcgct
     2881 gcagaaggcc ggaaacggtg cataccgcga cgagcgcgcc ggcatagagc gcgtcgagcg
     2941 cgcgcagccg ccgctgctcc tcctgccggg atacgacctc gatatcgcgc agcgcgagat
     3001 cccgtcccgt gagctgcaca acgctctcgc cgagcatcgc ctcaagatcg gcggcgagcg
     3061 ttccggcgtt cgtctcgtcg gtacagagaa cgaaaatggg ccggtcggtc tcgcggcgga
     3121 gcatcgccgc gccgagagcg cgcatcgcgc cgcccgattg ggtcaaaagg gcaggcaaat
     3181 agccctcctc gaccctcagg gggagggtag caagggaggg tgtttttcct atggctttga
     3241 ttatggcgtc cataagtata gagaatacac ctttatcgtg tcgtttgcaa gaaaaaagcc
     3301 tccccagaat gtggggaggt cagaacccct cagtcacggc tgcgccgtgc cggcgccccg
     3361 attcggggag ccaagtccgt tatccgaaat ctttcttgcc tcccctcgac aggggaggat
     3421 ggttttgccg caggggttta ttatataatt gattaccagg cgttatcgaa atcgcaccag
     3481 ccgtggtcct cttcgccgtc ctcgtcgggc agctcgccga gcacgaccgg cacctcgcgg
     3541 tactcgcccg cgcggtaaac gatgaggatc gtcttgctgc ccggcgtaag accgttttcc
     3601 gcggcggtga gatcggcggc ggtggctatg tccgcgccgt tcagccggat aatgatatcg
     3661 ccgctgcaaa gtcccgcgac actcgccggt ccgccgtcct ccacggcgta tatctgcacg
     3721 ccggggacca tggcgttgct gtccgagccg ttataatact ccgccgcggc gcagctcaca
     3781 gtctgaacga tcactccgag ataggggcgc tcgccggtgg gcgcgctcgt cgcggagggg
     3841 ggcgtatcgg catggaccgc cgcggagatc gccgcggcgc cggaaatatc cccgccggtc
     3901 tggaccgtct ccgatgaagg cgtggggacg gcggttttgg tattcacgac aggcttttcc
     3961 gcatcggaaa acgccgtggt gagcagcgag ccgagcacga aaaaggcaag acacagcgcg
     4021 cccacgcgca gcggcgagac cgttttcttc gtgccggagg atttttcttc cgaaaaagag
     4081 tagcagcgcg gcatattcgg cgcgtcgtcc tgccgtatat agcttgcgtc ggtaaatccg
     4141 acggatcgat cgcgcgtggg tttttcgttc atacctgccg ctcctctcca agatttttcc
     4201 ttattatagc gtggagttat gacaaatgca tgaacaaccc gggaattttc ctcggtattg
     4261 cattttttgc gcttttacgc tatgataatt gattgaaaaa aggccccctt gtgcaaaggg
     4321 ggctgtcagc ggagctgact gggggattgt attctccgga aaaacgacaa tccctccgtc
     4381 acggcgctgc gcgccgtgcc acctcccttt acacaaggga ggcttgaata tggaggaggc
     4441 aatctctatg gcaagcaaat atctggatat gtgtccgccg gtgctggcgt cgctcaaggc
     4501 gggaacgccg atcatcgcca tcgagaccgg atttttcatg cagctgccgt acccgcgcaa
     4561 tctggaggcg ctgcaggagt gcgagcaggc gttttaccgg cgcgactgcg tgccgtgctg
     4621 cgtcggcatc gtgaacgggc ggctcaaggc gggcctttcc aagcaggaca tggacacgct
     4681 ctgccgcagc ggcggcagct gcacgcgctc gcagattccc gcgctcgtcg gcggcggatc
     4741 gacgtccggc acaggcccct ccgctacgct ggccattgcg cgcatggcgg gcattattcc
     4801 ggtcatggcg ccgggactgc gcgattctct cgccgatctg gacgcgctct ccggctcttc
     4861 gcggctcgtg ttctgcggca aggtgtcgcc ggacaaggcg ctgcttttct cctcgcgcgg
     4921 cgtgccggtg ctgcgtctgc ccgcagagga gctcgcggac gcatatctcg tgcagcgtga
     4981 tcttgaggtg aacgaatgca cggtcatccc ctgcggcgat acgctcggcg atattgccga
     5041 gaaagcctcc gccgtggcga tggacatcaa gcgcaaggtc tcggcggtat aaaaaaatct
     5101 tcctcgttat gaggaaaacc cctccgtcaa aacctccggt tttgacacct cccctgttag
     5161 gggaggcaag taagtttttc gacggacttg gctcccctaa caggggagct gtcagccgca
     5221 aggctgactg aggggtttac aaccttctct ccgtctcgtc tcgttccgct ccctcgtcag
     5281 agggagcttt ttttgtatca gccgaatttt ccctgtacat aatcccttgt ccggctgtcc
     5341 tcggcttcgt tgaatatttt tgccgtttcg cccatctcca cgagcttgcc gagcaggaaa
     5401 aacgccgtct tatccgagat gcgcgcggcc tgctgcatgt tgtgcgtgac gatgacgatg
     5461 gtgtacgtct ttttgagctc gagcgccagc tcttcgatgc gcccggtgga gatcgggtcg
     5521 agcgcgctcg tcggctcgtc catcagcagc acctccggct cggcggccag cgcgcgggcg
     5581 atgcacagtc tctgctgctg tccgccggag agaccgagcg cgctcttttt gagcttgtcc
     5641 ttcgtctcgt c
//
LOCUS       DAWAAE010000133         5627 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6025_length_5627_cov_2.943647, whole genome shotgun sequence.
ACCESSION   DAWAAE010000133 DAWAAE010000000
VERSION     DAWAAE010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6025_length_5627_cov_2.943647"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2999..3008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          994 a         1883 c         1732 g         1008 t
ORIGIN      
        1 ccgggcgcgt gaggatgatg cggttaacgc ccttctcgcg gaatgccgcc accgccgcgg
       61 ccacggcgag atacgttttg cccgtaccgg cggggccgat gccgagcgtc acggtgttct
      121 cacgaatggc ctgcacatac gccttctggc cgagcgtttt cggtttgacc gggcggccct
      181 tcgccgtgat gcagacaacg ccgctgtcca gctcgcggat ctgatcctcc tgtccggagc
      241 ggatcatgcc gatcacatag cgcacgcgct gttcgtccac gttctcgccg cgcgcggcga
      301 gcatcagcag gttctgcacc gccttttccg cggataccac gtcctccggc tcgccggaga
      361 tcttcagctc cgcgtcccgg tccgtgacgc agacgttcca ctccttttca atgatgtgca
      421 gattctggtc gaacgagccg aaaacattga gaatgtcctc gacacgctcg gcggaaatga
      481 ttcgttccat ggattctcct gtattcttca tccggtcatg gccggagagt ttcttcgtcg
      541 atgcgctcct cgcactcgct gcgcagcgtg agcgtgagca tgccgttttc ctcatactcg
      601 gtaaagtatt ccgagagcac cgcgcccgtt tcgccgagcc gttcctgcag tgcagcgcgc
      661 agctcttttt cgagcgacgc gcgcaccgcc gcgcgggaga gcgccgtttc gcggagcgta
      721 tacggctgcg cggtctcaac cacggcggca gccggcaggg aaaacacgtc ttttacgccc
      781 agctgccata tccttgtaat tttatcacac tccgtacccg aaattccact atccgcgtaa
      841 aaatttattc tcgcgtttcc gataagcagc gcgaagcggt accgcgccgc gcccgtgctc
      901 tctttttccc gtgccgagag cggcgcggag gccgtcagtt cataccaggt atgcgccgtg
      961 acctcggcgc gcgcatggac ggtgcgcgtt tctccccggc gcgcggcgcg ctcgcagctc
     1021 acgagcgtct gccccgccgt caccgcgtcg ccgcgcgaaa cgagcgcttc gccctcaaag
     1081 acgcggatct ccgtaatgac gccggagcgc gacgccgtca cgtctgcggc ctgccgggga
     1141 tcggtgatct ccggcttttc cactgcggcg cgcacctcca cgctcgccgt gcttccccgg
     1201 acgttcaccg ccagccagct gagccccggc agctcggcaa gtgcacggga acggatacgc
     1261 tctccccgaa agcccggcca gaagcttccg acgcccacgc cctgctccgc gagcgtgtgc
     1321 agaatgagcg catccggcac atcggaatcg ttctccgtca cgcggatgtc ccatacgaaa
     1381 agcgacgaga cgagcaccgc cgcggcgact gctgcggcgc acagcgccgc ccaccgccgc
     1441 cggtgcagaa acttcttcca caccggtctc ccgcgcacgg acagcacctc gacatcgcag
     1501 ccgcagcgcg gcgcgagcaa ctttacctgc tccgcgtcgg cagcgaagat cagcgcccgg
     1561 cacgtgataa gatcgtccga ctccacgccc tccacggtga tgcgctcgcg gacgcaggcg
     1621 ttgatgagct tccacggcgc cgcgcccgtg atgcggatct ccgcgacgcc gcgcagcagc
     1681 cgctcgacgc gccctgtttt ccccttacgc atattccacc gcggcgatcc gcccggtcac
     1741 gatcagcgtg tcccggtcca tggcatgcag cgtcagctcg gcgccgagaa tacgcactct
     1801 cccggcggcg gcgctcacct cgacgctctg cgcgtcgtat ccgagcagtc cgcggtgatg
     1861 ctccaccacg gcgcgccgcc ggccgatgag cgtcacgcgc atcgcatcgg agaccgcctc
     1921 ctccggcagt tcgagcgaat cggcaagatc cgctaccgta tcgcgtagtt tcatagcccc
     1981 gcccccttcc ccgtcatcct atgagcgcgg caggacgtac atgccgcatg aaccgtccgg
     2041 taaggacata gagtagcgat atgatgaaaa gacgcttttt cggatacgcc gcgggcgcgg
     2101 cgctctttct tctcttcctc ttctcctctg ccgacgcccg gcagggcgcc gccgacggac
     2161 tccggctgtg gggaacgctt ctcgtgccgt cgctcctgcc gtactttgcc gcagcgggac
     2221 ttctcacgcg gctcggcgcg gtggacgcgc tcgcgcgccg tctcgccccg gccgccgcgc
     2281 agctcttcgg cgtgagcggc gcgggcgcgg cagtgttcct gctcggtctc tccggcggat
     2341 acccgctcgg ggcggcaagt atcggagtgc tttatcggga tggttcgctc cgaaagaacg
     2401 aggctgagca tctgctcgcc ttctgcgata attccggccc ggcgttcgcc gtgggcgcgc
     2461 tcggcgtggg agtgttcggc tccgccgggt gggggatgct gctctggggc atccacgcca
     2521 tctccgccgc cgctgtcggc attctcttcc gccggaggac gcgcggcggc gaagctccga
     2581 tcaaaccgcc gcggaggacg ccggactttg gcgcggcgct cggcggcgcg gtctccgccg
     2641 cagggcagac aatattgcag atcggcgcat atgtgatctt cttttctgcg ctgatcgcgt
     2701 ctctcggcgc gctgggcttt ccggacacgc tcgcgggcga gctttcgctt tgcaccggag
     2761 ggccgatctc cttcttccgt gcgctcttta ccggcgcgct cgagctctcg agcggcgtcg
     2821 gcgcgatggc gggtctgccg cttacgcccg gaagccttgc actgggcgaa tttctgctga
     2881 gctggggcgg gctgtgcgtc cacggacagg ccggagctgc cgccgaaggg ctgaaaatgc
     2941 gaaaacgcct cggaggaaag ctcctccagg gcgttttctc cgcagcgctc gcctacgcnn
     3001 nnnnnnnncc ggaatacgat acccacaacg gcagcgaggg cgaggaacac gaccgggtgc
     3061 agctttttca gcggcttcac cagattggtg agcacccaga tcacggcaaa caggatcagg
     3121 tttttccatt gaagaaggtc ggcttgctga tacgaggcgc ggtaggcgtc aaggttcaca
     3181 agcaccagcg cgaacaccga ccaccccgcc gcggcaatga gcccggtgga agccgggtgg
     3241 agcgtgtaga acgcggcatc cacatatttg ttcgtacgga aggcctgcag cgccatggcg
     3301 atgagcacga tgacgatcac gctcggggtg acaaggccga gagtcgccac gacgccgccg
     3361 agaacgccgg cgcagtcaaa gcccgcatag gtcgccatgt tgacgccgat ggggccgggc
     3421 gtggactcgg atacggcgag catgttcgcc acatccgctg tgctgaacca gccggtcttt
     3481 gcgccgagag aatagaggaa cgggagcgtg gcaaggccgc cgccgacgga aaacagcccg
     3541 accttgaaaa actcccagaa cagacgaagg tagatcatgc cttgccctcc tttccggtgc
     3601 gcagccatac gcgcacaacg atgccgagca ccgccgcgcc gacgatgaac accacggggc
     3661 tcacatggct gagcagcaca gagccgataa acaccgcgag gaacacggca aacgtaagct
     3721 tatccaccac ggactttttc cagagtttca cgatggagtt gaaaatgagc acgcacaccg
     3781 caacgcggat gccggcaaac gcattctgca cggcggcgat gtgcgcaaag ttgctcagca
     3841 gcgccgcaat aacggtgatg atcacgagcg acggcgaaac gatgccgagc gtggcaaaga
     3901 tcgcgccgga gagctttttc tccttgaagc cgacaaacgt cgctgtgttg accgcaatga
     3961 cgccgggcgt gcactggccg acggcgaagt agtccaacat ctcttcctcc gtggcccatt
     4021 tcttcttttc tacgatctcg cgctgcagaa gcggcagcat agcgtagccg ccgccgaagg
     4081 tgaacagccc gatccggaaa aacgcgcaga acagatccaa aagcagaggc aaggaaacga
     4141 ccctctttcc aaatatttat accgcacaca ttatagcaaa gcgttttccc gttggcaata
     4201 cgcgaaagct atggtaaaat gtctgcgggt gatcgatctt gacaataccg tctgaactga
     4261 aaaaaaatga aatatatccc gcccgcatca ccggctacac ctctgagggc gcaggcgtct
     4321 gccgcatcgg tgagcgcgcc gtattcgttc cgggaacgat cgtgggtgag gaatgggatg
     4381 tgcgcatcgt gcgcagcaca tccgccgccg tatggggccg gggcgaaacg cttcgctccc
     4441 catcatcgca ccgcgcggag ccggactgcc cggcataccc ccgctgcggc ggctgcgcgc
     4501 tgcgccacat gaattatgag gaggagcttt ctctgaaatt gcagcgtgtg aacgacgcgc
     4561 tgcaccgcat cgggaagctg gacttttccg tctccggcat tctccccgcg ccggaagacg
     4621 cccggcagcg ccgcaaggtg atcttcaatg tggcagagca aaacggccgt cccgtcgccg
     4681 ggttttaccg cgcgcgctcg cacgaggtcg tccccgtgcc ggactgccct gccgtaccga
     4741 aggaagcgct tgcgtgtgcg cagacagttc ttaactggat ggagcgggag catatcccgg
     4801 tctacgacga ggccgcgaag agagacggcg tgcgccatat cttctaccgt tcgtcgcatc
     4861 tcacggagaa ggctgtgctg acgctcaccg cgtcccgctc gccgggagaa aaggctctcg
     4921 gcatacttac ggatatgctg cgcgagcgct gcccggatgt aaccgggctc gtgctcaacc
     4981 gcaacacggc ccgcggcaac accgtgcttg ccggagagtt tgataccgtt tggggcagcg
     5041 aggtgctcac cgaaggactg tgcggcctca cgttcacgct ctcgccccgc tcgtttttcc
     5101 aggtgaaccc gccgcaggcc gagcggctgt atgaagccgc gctcggctat gccggtatca
     5161 cgccgggctt gacggcgctc gatctctact gcggcacggg gacgatcggc ctttgcatgg
     5221 cctcccgcgg cgcgcgcgtc atcggtgcgg aggtcattcc cgccgccgtg gaaaacgcgc
     5281 gggacaacgc agagcggaat catctttcgg acgagtgcga atttctctgc gccgacgcgg
     5341 gcgaggccgc cgcggagctt gcgaggcgcg gcatccgccc gcaggtgatc gtcgtcgatc
     5401 cgccgcggcg cgggctggac gagagcgtca tctcccacgc cgccgggatg cagccggagc
     5461 ggatcgtgta tgtctcctgc gaccccggca cgctcgcgcg cgatctcgcc tcgttcgccc
     5521 ggcagggcta taccctcgcc gccggcatcg ccgtggacat gttcccgcgg acgagccatg
     5581 tggagacggt agttcttttg tcccacaaaa aagccgacag ttatatc
//
LOCUS       DAWAAE010000134         5600 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6058_length_5600_cov_3.332732, whole genome shotgun sequence.
ACCESSION   DAWAAE010000134 DAWAAE010000000
VERSION     DAWAAE010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6058_length_5600_cov_3.332732"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1474..1483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2309..2318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3690..3764
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          939 a         1691 c         1720 g         1155 t
ORIGIN      
        1 tgtcgatctc gcgggcgacg atggccttct gctggttgcc gccggacatg ctgcgcacga
       61 ccgtcaccgg gccctggccg gaacggatgt cgtattcttc gatcagcttg tcggcgtatt
      121 tgcgcacctc gtcaaagcgg ataaagccgc cgtgctggaa ctgcggctca aaatagcgct
      181 gcaaaacaag gttctgctcg agcgtatagt caagcaccag accgtgcttg tggcgatcct
      241 ccggaatgtg gctcatgccc gtgagcgagc gctggcggat ggaggcgtgg gtgatgtcct
      301 tgccgcagag cgtgatcttg ccgccggtcg ccttttcgag accggtgagc gcgtagacaa
      361 actcgctctg gccgttgccg tcgataccgg cgatgcagac gatctcgccg cgccgcgcgg
      421 tgagcgatac gttcttcacg gcgtcatttt tgtgcagctg cgacggtacg gtgaggttct
      481 cgacctccag caccggctcg ccgggcttgg cgggcgtttt gtccaccacg agctgcaccg
      541 ggcggccgac catcatgcgg gagagctctt cctgcgtcgt ttcgttcgtg ttgacggtgc
      601 cgatgtactt gcccttgcgc agaacggtca cacggtcgga gacctccatg atctcgttga
      661 gcttgtggga gataaagagg atgctcttgc cctctttcgt cagcccgcgc atgatctgca
      721 tgagctcttc gatctcctgt ggcgtgagca ccgccgtcgg ctcgtcgaaa atgaggatct
      781 cgttgtcgcg gtagagcatt ttgagaatct ccacgcgctg ctgcatgccg acggtgatgt
      841 cctcgatctt cgcgtccagg tcgatcttca gaccgtagcg gtcagaaagc tcctgcacct
      901 ttttgcgggc ggtcttcttt tccagaaaac cgagcttcgt atcctccgcg ccgaggatga
      961 tgttatcgag cacagtgaac acgtcgatga gcttgaagtg ctggtgcacc atgccgatgt
     1021 gaagtgccgt ggcgtcgttg gggtcgttga tcgtgacctc ctgaccgtcc ttcttgatga
     1081 tgccctcttc cggctgatac aggccgaaaa gaacgctcat cagcgtggac ttgcccgcgc
     1141 cgttctctcc gagcagagcg tggatctcgc cctttttcag ctgcagggta atgtcatcgt
     1201 ttgccttgat cccggggaat actttggtaa tgtggagcat ctcgatcaca tactccggcg
     1261 tcgtgtccat gtccccacct ccctttctct ttctccagca gatttgctgc acaaggaatc
     1321 gcaatgtgca ttttaattat tatatatata ttacaaacaa attgcaagaa ttagctctga
     1381 attccaaaag aaaaacggcg ggaaggattt cccttcccgc acattgtctc tatgcctcgc
     1441 agagtttgcg cccgcaggcg caaataaaga tttnnnnnnn nnngcgtaag cgagtgcgct
     1501 gcggcgagcc gcagcccctc aaacaaaccc ctcaacggag tggggtttgt ttgaacgaaa
     1561 aaacggcggg aaggatttcc cttcccgccg ttttgtgcgg atggattaga tcacgttcag
     1621 cgtgaggttg tcggtgctgg cgaggttgtt gtagtcgctg tcaacaacga gcgtgccgtc
     1681 ggccatggcc ttgaccatct cgttgtactc ttccacggtc cagccggtca gagaccaggt
     1741 ggcggtgggc agaccaacag cgtcgtcctt ggcgccgagg gtggcgcagg tgccgcccag
     1801 ctcagcgtag gagccgtcgt aaaccttcgc gcaggcccac tgcacggaag cggcaaggcc
     1861 cttcatggcg gaggtgataa cggtgtcaga ctcgaaggac tggtcaacgt caacgccgat
     1921 gacaaagccg tcgttggcgc tggcagcctg agcgatggag gcgaacatgg agccgccgca
     1981 ggcgaagatg acctcggtgc cgttctcata ccagccgttg gccatggtct gcagttccgg
     2041 agaagcggag aaggatccgc cgtactgcca ggagtagttc atctcgacct tgacgccgag
     2101 ctcagccgcg gcagcgctcg cgccctgcac gtagccgtag ccgtaacggc agcaagccgg
     2161 gttggtgccg ccgccgccgc cgcagaagcc gagcttcgtg tagccttcct taacggcagc
     2221 atagccggcg agatagccgc actgctcttc ctggaacgcg atgcccagag tgttggtgag
     2281 ggggttgccg tcggcatcgg tcatcgccnn nnnnnnnntt catggcgtca tagcggtcgt
     2341 catcggtcgc ctgatcgccg ttggcgggct ggtagtactt gtagctcagg ccgttctcgt
     2401 gggcgtacag cttgacgccg tcgaacgtgc cctggttgaa ggacttatcc ttgagctggc
     2461 cgacgtcggt gacgaaggcg atctcatact tgccgtcggc ggaggtcatg gtgtcctcaa
     2521 tgtcgtcggc gctgacggtt gcgggctctt ccgtcttggg ctcttccgtg ggctgctcag
     2581 tgggctcttc cgccggaact tcggtggcag cgggctcctc ggtcttgggc tcttcggtcg
     2641 cagccggggc ggcggtggtg ccgcaggcaa cgagggcaaa gaccatgacg agagccagga
     2701 tcagggcaag gatcttcttc atgtagaatc tttcctcctt aataatttgt cccttttcgg
     2761 gactttcgca acgatgatta taccatgtcc gggcggtata tgcaaggtct gtcctccgcc
     2821 tcaccgtcgg aaatgcgggt cttttttatc tgttttcacc gcccgtccgt gcggcgattc
     2881 caagtgtttt tcagctgcgg acagccgctt ccagcgccag ctccatcatc tcgcggaaag
     2941 agttctggcg ctcctcggcg gtcgtctcct cgccggtgat gacatggtcg gaaacggtgc
     3001 agatggtgag cgccttcttg cccgcgcgca tcgcgttcag atagagcgcg gcggcttcca
     3061 tctcgatgcc gagcacaccc attttgatcc acgcggcatt cgcggtcttg tcatcgtcgt
     3121 agaacgtgtc cgaagagagc agattgccga cggcgtagcg cagtccgcgc tcttccgcga
     3181 tgtcggcggc ggtgcgcagc aggccgtagt ccgcgatcgg ggcgatcgcg ccgcggcagc
     3241 cgaactgtgc ggcgtaagcg gagttggtgc acgcgccctg cgcgaggaca atgtcgcgca
     3301 catgcagctt ttcgctgagc gctccgacgg agccgacgcg cagaatgttc tccacgccgt
     3361 agaagttgaa gagctcatag ctgtaaatgc ccatggacgg catacccatg ccgctggcca
     3421 tgacgctcac cggcacgccc ttgtaggttc cggtgtagcc ctggatgccg cggacgttgt
     3481 tgacgaggcg gcagtcgttg aggaagtttt cggcgatgaa ctgcgcgcgc agcgggtcgc
     3541 cgggcatcag tacggttttg gcgaaatcgc cgggggtggc gctgttatgc ggtgtggaca
     3601 tgcggatcgt ctccttttta atctaattat gtgtaagttt tactgcttct ctttctgcgt
     3661 ccgttcggcg cgctcgcgcg cgatgcgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgtt gaccgtggcc
     3781 ttcgagccgc actcgcagat ggttttgatc tcggtaatgg agtcggccag ctcaaaaagg
     3841 cggcgcgagc cggggaaaag gtgcgtgaga aagtcggtgc gcagcccgaa gcagagcacc
     3901 ggcagattct cctcgtccac gagctgccgc agctgctcga tctgctcggg cgtgaggaac
     3961 tggcactcgt ccacgatcac cacgtcgtga tggccgcgcg cagcgtaaag cgcgcggata
     4021 tcgtcctccg ggcggacgac gtcggcggtc gcctccagcc cgatgcgcga gcggagcagg
     4081 gtctccccgt cgcggcagtc gacggagggc ttcagaagcc acacgtccat gccgcgctcc
     4141 tcataattga atttggtgat gagcgcctga gcggtcttgc tcgagcccat cgcgccgtat
     4201 ttgaaataga gcttcgccat ttctgcgggc atctcctctc ggtttcttca agaaaagatt
     4261 ttaccgcaaa ccgggacaca agtcaacgag agcgcatacg ctggaatacg attttgaata
     4321 ggagtggtgc attatgcgct ttgccatcac cggcacggac gcccggtttc ccccgctgcg
     4381 gaagctcctg cttgccgacg gccacgagat caccgacccg gcctccgccg acataatcat
     4441 cccgccgccg tgggatccct cggcgcggta cgctcatttg gaggaatatc agattgcgaa
     4501 cgcccgcctc accgccgagg gcgccatcgc gctgctgcgc ccggaaacgg ggctttccgg
     4561 cgcgcacata ctgctgctcg gctacggccg catcgcccgc ctgctcgccc gggagctgca
     4621 gaaggccggc gctctcgtca ccgccgcggc gcgctccggt gagcagcgcg catgggcgga
     4681 ggcggagagc atcgaggctc tgccgctcga cgcgctctcc ggcgcgctcg accggtttga
     4741 tgtcatcatc ggcacgatcc ccgcgccggt gctcaccgag ccgctgctcg cgctcgtccg
     4801 gaaggacgcg ctgctgctgg agcttgcctc cgcgcccggc gggatcgatg ccgccgcggc
     4861 gcacgagagg ggtcttcgct acatccgcgc gcccggtctg ccggcgaaat acgcgccgga
     4921 gcgcgccgcg gtcattctgc gcgacgcggt gtacgccgcc gccgcggagc ctctcccccg
     4981 tctcggtctc gccgtcacgg ggtcgcactg cacattttcc cgcgcgctgg aggcgttccg
     5041 gcctttgaaa cgggactata cgctcgtgcc gatcctctcc ggcgcggcgg cggggacgga
     5101 tacgcggttt ttcgccgcct ccgccttccg cgcggagctc gaggcgttct gcggccggga
     5161 agcggtggac acgatcgtca aggccgaacc gctcggcacc gcgcagcggc tcaacgcgct
     5221 gctcgtcgcg ccgtgcacgg gcagcacgct cgcaaagctt gcccgcggcg tcacggatac
     5281 cgccgtcaca atggcgtgca aggcgcatct gcgaaacggc gcgccgctca ttctcgccat
     5341 ttccacaaac gacggtctct ccggctccgc ggagagcatc gcggcgcttt tgcagcgtaa
     5401 gaacgtgtac ttcgtcccct tccggcagga cgcgccgcac cagaagccct tctcgctcca
     5461 gagcgacttt gaccttctcg gcgaaacaat aaaagcggcc atggaaggcc gccagctcca
     5521 gccggtgctg ctgtaaaatg ctgcgccccc tgaaaaacag ggggcgcatc tcagtctgtt
     5581 gaaaaagcct cgcagagttt
//
LOCUS       DAWAAE010000135         5593 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6067_length_5593_cov_3.404478, whole genome shotgun sequence.
ACCESSION   DAWAAE010000135 DAWAAE010000000
VERSION     DAWAAE010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6067_length_5593_cov_3.404478"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1013 a         1735 c         1885 g          960 t
ORIGIN      
        1 ttacgccgta ggcgtcggcc ccggcgatcc catgtggatg acgcgcgagg cgtgcaccgt
       61 gctggaggcc tgccccgtga tcgccgcgcc gcagaccgct tcgggcgaga tgctcgcgct
      121 ttccatcgcg cagggcgcgg cagagctcgg cggaaagacc attctgccgc tctcgttcgc
      181 catgtcgcgc gacgccgccg tccgcgccga aggctatgcg cgcgccgcgg acgccatcgc
      241 gccggagctc gacgccggac gggacgtcgc catggtgaat ctcggcgatg tgtccatcta
      301 cgccacggcg tattatgtgc tcgacgagct gcgccgccgc ggatgcgata cgcgcatggt
      361 ccccggcgtt gcgagctttt ccgccgtggc cgcggcgctc ggccggagcc tcacggagat
      421 ggagctgccg ctgcacatct atcccgccgg agcgtccgat ctcgatgccg cgcttgccca
      481 gcccggcacc aaggtgctga tgaagtccgg caaagccatc ggcgagaccg ccgcggcgct
      541 ctcgcggcac ggtctggcgg aggattccgc catggtcgcc gactgcggcc tgcccacgca
      601 gcaggtgttc gagacgatga ccggcctgcc ggagaagatc agctattttg ccacggtgat
      661 cgtgccggac agcaaagaat aagagcagcc ccgccgcctt tctcggcgct gcccgtcata
      721 aaagaaaatc ctctctctgc acagcgcaga gagaggattt ttcgtatgtc cttatttgag
      781 atcgggattg gagacctcgg tataatccgg cgtcggggca gccgccggag ccgccgtcgc
      841 gggagcgacc ggcacggccg gatcggcggg gttgaccggc gcggcgggcg ttgccgtcgc
      901 cgcgggggcg tcggccgttt cacgcagcag ctcaccggcg gcccagccgt agcggccgtc
      961 ggcggtgcgc aggagcaccc agccattctg cttggcgagg atctccaccg tgtcgcccat
     1021 gttgagcgcc atgttctcac cccagtccat gccggggccg tagtgaacgg caagcatgta
     1081 cgggatgacg gcggcggtct ttgcctcgtc ataccagaac tcatccgccg gacggtagac
     1141 gttgccgagc atctcggcgg tgatcgtgtc gcgcaggagc ggggcgggcg tgggcgtcgg
     1201 ttccggcgtc ggctccggcg tagcggtggg ctccggcgtc agctccgggg tgggtgtggg
     1261 cgcttcggtc ggcgtgggcg tcggcgtctc ggcggcccgc tgcgcgcgca gatcgtcaag
     1321 ctgcacctgt gcggccgtca gctcctgtga gagcgtttcg gcgcgggtgt tcgcggtctg
     1381 cagacgcatg taaagcaata cgatgatagc ggcaaacagg agaaggagca caacgagtgc
     1441 gatcagaaac ggcttgagcg gatcgcggta ggtctcctcc ggggactcgt cttcgtattc
     1501 cggataatct cggggcatgg tatagcctcc ttatcactca cgaggaatct ttgttattct
     1561 atcagacttt gccgcattgg tcaagatttg gcggcaaatg ggctgatttg gcagataaaa
     1621 cggccctccc gccatgaaag cgggagagcc gtgaaggggg taaggttatt tgcgcagcgt
     1681 aacggtgaat tcgtcgtcgc cgttatcggc tgcggtcacg gtatagccct gactttcgcc
     1741 gaagcgcgtg acgttttcga cggcgcactt gttatcgaca agcacctgca gctcgccggg
     1801 agcgtccgcc ttgatggcct tctgcgccat gatgaccggc tgggggcagg caaagcctct
     1861 cgcatcaacg gttttcatgg gcttattcct ccgccttctt cgtgaagagc agggagacca
     1921 cgagcaccac gacgaagccg acgatcaccg cgatcttgcc gttgccggtc gggccgtcgg
     1981 cagaggaggc gagcttcagg ttgtggcaga acgccgcgcc gcacatcatg ccgagaacgg
     2041 tgacggcgct gtcgctctcg ccctggccgg cgagaatgag ctgacgtagc gggcagccgc
     2101 cgagcaggac gcagccccag cccatgaggg cggtgccgag aaagttccag agaccatcgt
     2161 tgtgcgcaac gggctggccg gtgaagccgg ggttgaactt gccgagaatg agattgccga
     2221 cgagcaccgt gagaatgatc gtcacgaagc cggcgacaag gccgaagtcc ttgaacatga
     2281 cggcgttgcg gatgccgccg accatgcaca tacggctgcg ctggcagagc gcgccgacga
     2341 cgagacccgc gacgagcgcg agaccccacg cggcatgcat gctgccggga cctttttcgg
     2401 agaacttgaa gatcgccgga gcggcgagcc agaggacgaa cagcagcaca taaacgaccg
     2461 ggaaggcggc gccttcggcg agcgtctgct tatagctgcg gccgagcgtg aagcccttct
     2521 tgaggaacac aacacccacg agaatgccca cgatgaagcc cacaagaccg acaagggcgt
     2581 tccagtcgcc gccgccgata cggatgacca tacgcagcgg gcagccgagg aacatcagcg
     2641 cgccgatcat aacgaacacg ccgagaagaa agcggatcat cggagaagag ccgccgcggc
     2701 ccttgaattc cttcgccgcg accgccgcga tgagcgagcc gagcaggatg ccgacgatct
     2761 ccggacggaa gtactgcacg acctccgcgc cgtgcagacc gagagcgccg gtcgtgtcgc
     2821 gcaggaagca ggcgatgcag aagcccatgt tgccggggtt gccgtgcgcg cccaaaacga
     2881 gcgccgcaat gccgatcaca agtccggtga cgaggatcag ccagttattt ttgaaccagt
     2941 tgcttttcac aatttttccc tctctctttc aaaaactgtg ttttaacggc ttcattatac
     3001 gacgtctctc cggaaaatgc caaacggtaa atcggatatc gcctgtggct ctatcggaga
     3061 aacagataga acgagtcgtc aatgcagcgt ccggcgatca agcagaaagt caatgaacgc
     3121 gcgcgcggac tttgtcgggc ggcggtcgcg ggagcggatc atgtagatct cgcgctcgtt
     3181 gtttttcccg acgagaggga aggagagaag ctcctttgtc gcacccgcgg caagaccgga
     3241 gacgacggcg cagccgagcc cgcacgccac ggcgcgcagc acggcctcgc tgttgtcgat
     3301 gcgcgcgacg atgttgagcg cgccggagtc gatgccgttt tcggtgaaga atttctgccc
     3361 ggcgagctgc gtgccggaac cgccctcgcg gaagatgaga ggctcgccga gcagcgcgtt
     3421 gcccgattcc ccggcgtcga acatggcgcg gtagcgcggc gtgtcgggcg tgacgagcac
     3481 gagcggatcg gtggcgatgc agtcgtaggt aagctcgcgc gtgttcagca ccgcgccgac
     3541 gaagccgatg tgcgtttcgc cctgcaatac ggcggcgtgg acggcggcgc tgtcgccgct
     3601 gcggagcaga aagcggaaat ccgggttttc ccggtgaaac tcgctgatga gctgcgggag
     3661 gatatactgc atcgggatgg acgacgccgc gagcgatacc tcgcgggagg tctgctcctc
     3721 cagctcgcgg gagagctcgg cgcagctctg gagaatggtg tgcgcatggt cgtatacgcg
     3781 ccgcccctcc tcggtgagcg tgatgcgccg cttgccggag cggacgagca gcggcacgcc
     3841 gatgtccttt tcgagcgcga gaatgtggcc gctcacggtg gactgcgcga gatagagcgc
     3901 ctccgccgcg gcggtgaagc tgccgcgttc gaccacggcg cagaaggcct cgagctgttt
     3961 gagattcagt gtctgcatgg cgctctcctt attaaagctc gcgcaggtac acacccgcga
     4021 tctcgggaag ccccggttcg ttttcaatgc gctcgcgttc ctccggcggc gcggcccacg
     4081 cgatgccgca gtcggcagtg atgctccgcg gcgcggggat gagccgtccg ggaatgtttc
     4141 gctcctggca gagcttttcc gtcgccatcg cagcggcggt cgtgtggaac gtgaagatca
     4201 cgtattcttt cttttcgcgt actctcatag ggcaatgtcg cggacggcat ccgccgcctg
     4261 ttccgcttcc tccatcgtat tgaaccagcc gaacgaaaag cgcaccgcgc cctgcgaaac
     4321 ggtaccgagc gcctcgtgca tccgcggcgc gcagtgcgcg ccggagcgca cggcgatgcc
     4381 gtattcgtcg gagagaattt ccgccgcctc gccggaatcc atgtcgccga tgttgagcgt
     4441 gacgatcggc gcacggtcct gcgtgaagtc gccgtacacc gtgacgcccg gcagggcgga
     4501 taccttataa taaaaagcgc tcataagcgc ggtctcatgc gcgtggatgg cggcgggggt
     4561 gatctcgcag aggaaatcca ccgccgcgga cagccccgcg atgccgtggc cgttgagcgt
     4621 gccgctctcg agccgcgtcg gatattcctc cggctgttcg cgccggtagg actgcacgcc
     4681 cgtgccgccg gtcttgagcg ggcggatctc cacgccgggc gcggcgataa gcccgccggt
     4741 cccctgcggc cccatcatgc ccttatgccc ggtgaagcag aggacggaaa cgccgtcgcg
     4801 ctgcgcgttc aggtcaaagc tgcccgccgt ctgcgaggcg tcgaggataa acagaaggcc
     4861 gtgctcgcgc gcaaaagcgc cgatgcggga tgcgtccagc gcgttgccgg tgagattgga
     4921 ggcgtgcgtg cagacgatcg cgcgggtgtt cggccgcagc gcgcggggga gatcggcata
     4981 atcgatgcag ccgcgcgcat cggcgcgcag gaacgtgacc tccacgccct cctgctccag
     5041 ccggtagagc gggcggagca cgctgttgtg ctcgagatcg gtcgaaatca cgtggtcgcc
     5101 gggccggaga agcccgaaaa tggcagtgtt gagcgcctcg gtcgagttct gcgtgaagca
     5161 cacgcgcgac gggtccgccc cgccgaaaaa ggcgcagagc ttttcgcgcg cgccgtatac
     5221 aacgcgcgca gcggaaagcg cgctctcgct cgcgccgcgg ccgacgttgc ccagcgagca
     5281 cagcgcctcg ctcaccgcgc gcaccacgcc ctccggacgg cggagcgatg tggcggcgtt
     5341 gtcgagatag atcataaaaa accctcccgg agatattatc cccaagaggg tagcacgttt
     5401 gccttatttc gtcaagaaat ccgatagtaa aatcggaatt aagaatgatc tttcgtttcc
     5461 agaaacgcgg cgtgcagaca gcggagggtt tcaaggctct gtgcctctgg aacatgcacg
     5521 gtaaggcgcc cgtcggtgag aagcacggcg tccggggaga gccccgcctc acgcagcgcc
     5581 gcggccgccc gtt
//
LOCUS       DAWAAE010000136         5545 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6112_length_5545_cov_2.969763, whole genome shotgun sequence.
ACCESSION   DAWAAE010000136 DAWAAE010000000
VERSION     DAWAAE010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6112_length_5545_cov_2.969763"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1004 a         1626 c         1681 g         1234 t
ORIGIN      
        1 gtgcacgatg tggtagagcg tcctgccgtc ctcgcgccca acatggtctt ctgcgtggat
       61 gtcggcgtgt accacgagga atggggcatc ggcttccggt tggaggataa ctgcctcgtg
      121 acggaaaccg gctgtgaaaa cctttcggcc gctattccgc gcaccattgc cgatatcgaa
      181 gcggagatcc gccgcatctg acctatggag cgaatattgc tttggcttct ggccgccgga
      241 gcgcttatcg gcgggatcga ctgtcttctc ggcaaccggc tgggattggg agaacgattt
      301 gaggagggtt tccggctgct gggccccacg gctctctcca tggcgggcat tctctgtctt
      361 tctcccctca ttgtccgggt actttccctg acgctctctc cgctctggca aaggatcggt
      421 ttggaccccg gaatgctggg cggttttatt cccatcgata tgggaggcta ccaggcggca
      481 gtcgatcttg cctccgatcc gaccgtcgga ctttacgcgg gcattctgat ctccgccaca
      541 acgggatgca cgctggcatt taccattccg atgggcatgg ggcttctctc tccggaggac
      601 acgaacgctt tttcccgagg gatattgatc ggtctcattc ccatgcccgc ggcgcagata
      661 ctcggcggtc ttgccggagg gatgcagttt ctcccccttc tgaaagagag cattccgatc
      721 ctgcttttct ccgcggcgct gatgctctgt ctccattttg ccccgcagaa aagtctgaaa
      781 gtattcggcg gctttgccaa gttccttcgg gctctttctc tgatcgggct gacgctcggc
      841 gcggtgcagt atatcggcaa tataaagctg ctgccttccc tgatgccgct ggaagaggca
      901 atgcaggtgg tcgcgtccat cgggatcgtc atgctgggga gtctccctgc ggcggagctt
      961 ctccggcgcg gcctttcccg gccgttttct gctttgggca gaaagctgca tatggatgac
     1021 cgcagctttg ccgccctgct cgtcggcttt gtcagcgcca ctccggccat cagcatgatc
     1081 aagggaatgg atccgagggg caaagttatg aacgccgctt ttcttgtctg cggcgcttcc
     1141 gcgctggcct cgcatctcgg ctttaccgtg gccgtcgagc ggagcctgat ctttccgctg
     1201 cttctcacca agctgaccgg cggtcttatg ggtgcggctt tggcgcttct gctgacaagg
     1261 cgatccgccg tgaattcccc ggtttcgtaa atgcggcgtc cccgaacgat acgacatata
     1321 agaatgcggc gcggtttaaa gaaccgcgcc gcattctttt actattcctt aattgatgtg
     1381 aagggtgtcc tccgccacga tggtggcgat gttctggttg ctgtaagtgc cgttgaagtg
     1441 ccatgtcacg ttcaccggca cgtccgtgcc ggacgtgaca tccacgctcg tgctgccgag
     1501 gaaggtctgc gtcaggggga cattatcaag gcggatggcc ttggaggacg cgtacgacac
     1561 attgccgccg acggtgaaca caacgtcgct ggtgcggtcg cccgttacga gcgcgtacga
     1621 ctcgacgtaa gtatcgagct tgagcgtttt cgtgtcgccg ctgccggtca ccgtccactt
     1681 gacgatgatg ttgatgtacg tccccgtgtc cgagcgaaac tcgccgctct ggtcaaggac
     1741 gggcgccgcc gtcggctgca cagtgggtac cggcgtttcg gtcgggacgg gcgttgctgt
     1801 aggaacagcg gtcgggacgg ctgtcggaac aggtgtcccg gtcggctccg gcgtcgccgt
     1861 gggggacgtc gtggccgtcg gttcgggcgt tgcggtcgct gtcgtcccgg gattttgcgt
     1921 atccgaagca ttgtttttcg atatacggaa cacaacaaaa atgccgacag cgacaagcag
     1981 gaaaacgagg atcaaaatgg gagccgcgga ttttttcatg gagaatacct ccctatcatt
     2041 atttcatcag gagatcgatc gccttgtcca gttcagaaag cgtttcggta tccccttttt
     2101 ggacggcgtc gatgacgcaa tggttcatat ggtcgcgcaa aattattttg cagatgttgt
     2161 tgatcgcgct gcgcacggcg gcaagctgta cgagcacctc ggcgcagtcg cgcccgtcgt
     2221 cgatcatgcg ctccacggcg tgcagatgtc cgatcacgcg cgccagacgg ttctttacgg
     2281 ttttggtgtt ctggtgcgta tgcgcgtggg agtgagaatg caccgttccg tcggcatgga
     2341 cgtgaacgtg cccctccgcg gtatgcatgg attccgtatg ttccataact tctcactcgt
     2401 ccttcgtcat gtgctgcgcg gcaacgcgca gcatcattcg tttcgtttcc ccggaatcga
     2461 actttacctc caccagcgcg tcgccgccca tcggcgtcat cttttcgatc acgcccttac
     2521 cgaacgcctt atgtaccacg cggtcgccct tttggaagtc cggcgctttc ggcgcggaca
     2581 ggacagtctt cggcttttcc ggagccgcgc gctttgccgc gccggtccgt ccggtgcgtg
     2641 cagcatagct cgtgccaaag ctgccgggcc ggtaggacac ccctccgccg gaatagccgg
     2701 aggcgcctcc gccccacgtt ccgcttcttc cggagatgaa tgtgccgtcg tcatcccaga
     2761 gattcgcttt ctttttcgca agcccctgcc ggtcgagcag cgtctggggt atctcctcgg
     2821 tgaagcggga cggaaggttg gccgatgtgc ggccatagag catgcgctgc gcggcgcagg
     2881 tgatatagag cttttcccgc gcgcgggtga tcgccacata gcagagacgg cgctcctcct
     2941 caatatcctc gctctggccg atggcctgca tgctcgggaa caggccctcc tccgcgccga
     3001 taatgaacac aaccggaaat tccagaccct ttgccgcgtg catcgtcatc atgacggcgg
     3061 catcctcggc gcggtcgtaa ttgtccagat cggtatagag cgcaacctcg tccagatacg
     3121 aatagagatc gaggtcgggg ttggcttcca cgctcttcac gatgctggac ttgagctcgg
     3181 cgatgttttc aaggcgagtg acattttcgt cgccgccttt cgtctcgagc gccagcgcgt
     3241 agccggtgcg ctcgaccacc gcgtcgtaaa actcatcaag cggcagcgca gaaaccgctt
     3301 cctgcagctc cgcgatcata cggcagaact cctccatccg ctttccggcg gccatgccgg
     3361 tgcggtgaga cgccgtggtc atcgcctcaa aaacggaaat attctccgcc gcggcgatct
     3421 cgcggatctt ttccagactt gtcgccccga tgccgcgcgg cggctcgttc acaatgcgca
     3481 gaagccgcgt ttcatccgtg gggttcgcga tcacgctgag atatgccaga atgtccttga
     3541 cctcggtgcg gtcaaagaag cgcgtgccgc cgaaaatgcg gtacgggatg gagcggcgct
     3601 tgagcgcaag ctcaatgctg cgcgactgcg cgttcgtccg gtaaagcacg gcgaaatcgc
     3661 gcatgggccg gcgtgcggag gcgatggtac gcgcgacaaa gtcggcctcg tcatcctcat
     3721 tccgcgcctc gaagagcgtg acggggtcgc cgccggactg cgccgtccag agcgttttgc
     3781 ctttgcgctc gtggttgttt gcgatgacgg cgttcgccgc ggcgagaatg ttgccggtcg
     3841 agcggtaatt ctgttcgagc cggatgacac gtgcgtccgg gtactgcttt tcaaaggaga
     3901 ggatattttc gatcgtcgcg ccgcggaatt tatagatgct ctggtcgtcg tcgccgacaa
     3961 cgcagatgtt ccggctgccg ctcgccatga gcgcggcaaa gagatattgc agatggttcg
     4021 tgtcctgata ctcgtcgatg aggacgtatc ggaatttttt ctgataatac gcgagcacat
     4081 ccggctcgtc ctgcagaagg cggacggtat agtatatgag atcgtcgaag tccatagctc
     4141 cggcgtcccg caggtgtttg gcataggctg catagatctt cgccgtgcgg atgcggcgga
     4201 tatcaccgga gcgctcctcg gcggcgacgg cctcctccgg cgagacgagc gagcccttgt
     4261 atcggcccgc cgcggcaagc atggccttcg gcgggaacac cttgtcgtcc atgttcatgt
     4321 cgcgcagaat gcgcttcata acggagaggc tgtccgcctg atcgtagatc gtgaagctgt
     4381 tcggaaagcc aatacggtcg gcgcagcgcc gcagaatgcg cacgcacgtg ccgtggaacg
     4441 tgagcgccca gacatcgcgc ccggtctctc cgagcttggc ttccagacgg tttttcagct
     4501 cgtcggcggc cttgttcgta aacgtgattg cgagcacctg ccacggcgcc gcgggatcga
     4561 cggcgcacag gcggcggata tcgtccgtca cgtccgtgcg tccggctgcg gcgtcctcga
     4621 gcgcggcgag catcgtctcg tccgcatcgg gcggaagctc tctcgtgtcc gcaccgcggc
     4681 caaattgcag gatattggcc acgcggtgga tcaagaccgt cgttttcccg ctgccggcac
     4741 ccgcgagcag aagcagcggg ccttccgtcg tcagaacagc ttcgcgctgt tccgggttca
     4801 gattggcata ttctttttca atgatctgcc gccgcgcggc ggcaaaccgg cgggaaaagt
     4861 cctcggtcat ggagagatat cctcggtttc ttagtctttg aagaacaggt gcttgacgcg
     4921 cttatagata aagaacgtga tcgcgccgtt ggtgcccgct ttgataagat tgaaaagcag
     4981 gatgaacggc atgagcgaga gcacggcgga caccgtgccg ctgttcaccg cgcccatcag
     5041 aaaatacggc gtgatgacga gattggcaaa gaacatgcac acgaccatcg tcagcgtgcc
     5101 gatgccgatg gcgaccgcgg ccattttctt cgttttgtgt ttccggtaga aaagactcga
     5161 cgagagcaca tacgcgcccg ttgcgacaat gtgcatgatg atgccgtaga tcccgctctg
     5221 ggcgctcacg gtaaagccct gaatgaatgc ggcgatcagc gtgacgatga atcccgcagc
     5281 tgggccgaac gcaaagccgc agatcagaat gggaatatcc gccgggtcat attcaaggaa
     5341 cgcgaccgcc ggaaaaatcg gaaaatggat cagcgcaaca aagcagacgg agagtgccgt
     5401 caggatcgcc atcgtcgtca gtttacgggt gtttgtcatg ggaaaggccc ccttaaaaaa
     5461 aattcgtccg aagaaagcca ttctccgggc gcagcacaag aatgtaagga gagccttacc
     5521 gcttcttctt ccatccagac tatac
//
LOCUS       DAWAAE010000137         5473 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6200_length_5473_cov_2.894426, whole genome shotgun sequence.
ACCESSION   DAWAAE010000137 DAWAAE010000000
VERSION     DAWAAE010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_5473_cov_2.894426"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3334..3343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1137 a         1515 c         1596 g         1215 t
ORIGIN      
        1 gccctatggg gcagggaatc ctttttggta cgccagacgg gatttgaacc cacgaccttt
       61 ggctccggag gccaacactc tatccagctg agctactggc gcatttatca gcttaattat
      121 tatagcaagc cggaacggaa aagtaaagag gaattttccc atggttttcg acaatggcgc
      181 gaaacaggaa agaatagaat tgaccggctg cttttcaggg cagccggtct cgcatggggg
      241 atcaacaaag agaaaaggat ggagatggat cgatatataa caaccgggaa aggggagccc
      301 cggtgtgtta ccagatttat tttacaagct cgcggcccag ttcgcggcag gcttcaagct
      361 cgatgtcgcc gggggtctcg cagcagatga cgctctcgca cacgagagag atgttcgcgc
      421 cgcggcagtc ctgctcccag gtgcgcatcc attcgccgtc gccccagccg taggagccga
      481 agagcgccgc gctcttgccg acaaactgct tcttgcagga ggagaacatc ggctcaaaaa
      541 cgtcctcttc gagaacttcg gagcccatgg ccgggcagcc gagcgccacg gcgtcaagcc
      601 cggcgagatc ggcggggctc acggcgtccg cgctgagaag ggagacctcg gctccggctc
      661 ctttcatgcc ctcggcaacg gcgtttgcca tggcctcggt gttgccggtg ccgctccagt
      721 aaataacagc tgcttttttc ataacagaat cttcctttcc ggcacatcac accgcgacgg
      781 cgagctcggc gataccgcag ccggcttcgt accgttcgca atagatgtcc gtgcattttt
      841 tatatttggc aaaaagctct ttcgctttct tctcgtcgcc gtagaccttc cacaggccgc
      901 tgagcttgta gttggcggcg cgattttcga taaagccctt tacatcctcg gggggatagc
      961 tgagaaacag cccgatctcg tgcgggaact cttcgccgct gcgcagcttg cgcgtgagcg
     1021 ctgcaagaca ttttccgcag ctttcgcagg ggtaaccctc ctgccggagc agctcggcgg
     1081 cttcactgtt ttgcagatcg cgggaaagct ccgctgggcg gaacagataa aggagaacgt
     1141 tcttttcgga ataacgcagc ggcagcaggc atagcccttt tggagcaaag cagcggttaa
     1201 gctgccgcaa agattcttcc acgcttttgc gggaagtata cgcacaggtg aacaggcttc
     1261 ccgtcttgat ccccgccagt gtgggtgcgc ccagtcggac gatcacttct tcagacattc
     1321 gtgagtcctc cttccgggcc gcaccgcaca aaggggtgag agataatgtg acatggtgga
     1381 aaagcataca ttcggggaag cgtttccctg tttgcaaggt tatcatacgc taaccacgat
     1441 gccgcatccg ctccaaaatg agcgaacgcg gctccaaaat gttgtttttg tgttggaatt
     1501 cggatcagac cggcgcgcag ctgtcggcgg tcgcgccgct gcggtaggcg ttgggggata
     1561 cgccgatgac gctgcggaac gcacgggcaa atttgctggc gttgtcgtat ccgaactgcc
     1621 cggcaatatc gagcaccgtc cggtcggtgg aacggagaag ctccgccgcg gcgtgcattt
     1681 tctgagcgcg cagaaacgcc gccggagaca tgccgtaaac gccctggaaa ctctgcaata
     1741 tctgcgtggc cgaggcgtgg aactttgcgg agagctccgc cgttgtgggg cgctcctccg
     1801 ttgcggagag cagatactcg ctcacggcgg aggcaaggcg ctcctgcgcg ccggaatagg
     1861 ccgggcgccg ccgctcggtc ggtagcgcgc tgaggaagag cagcagctcc aaaaccttca
     1921 ctttaaaata ccccttgcgg atctcctccg gcacggagta cagctccgaa aaaatatgct
     1981 ccacgctcgc ggacgagcgg gcggcaaagt agccgccgtt cgcgcagaac ttttccatca
     2041 gcgccgccgg gcggacgttc acgtccgaga gcaggcacga aagacactcc ggcgcctgcg
     2101 ccggatcgat cgtcacggtg atgccgtggt aatgcccggt ggggaagcgc gcggcctcgt
     2161 cggaggcgtc gctgcgtgaa acggccagat cccccggcgc gagaaaataa tattcgttcc
     2221 ccagctgctg ctcgagccgc ccctcgcggc agtggctgat gcgcatgagc gagatccccg
     2281 gtgtgtgttc gagccggcac tcgtgcgcgt gtatgtcgca gtacgcgatc tctatgcccg
     2341 gaaataccgg ataggcagtc acaacggcct cgccgtccgc gctctccgga tgccggacga
     2401 accgcccgtc cgcctccgcc gcggcgcagc cggtgcaggc gttctttttc tctttcatgg
     2461 gtttcaatac atctcctgtt agctgatgct aaccataaaa tagcacgatt ggactgaaaa
     2521 tgcaagaatg gcgtcttgac ggcgggcggc gcggcggata tttcgccgct ttctcccgta
     2581 tttccttgac ttttttacag caaagcttta aaataataaa actttgtgaa aacaggaaaa
     2641 cgagcaggtt ttccggtcga gagggaatcg atgaagaaaa agaatgtaaa gagcttttcg
     2701 actcgctggg ggttcatcct cgcgtcggtc ggctcggccg tcggcatggc gaacgtctgg
     2761 ggctttccga ataaaatggg cagcaatggc ggaggcgcgt ttctgctcat ttatattctg
     2821 ttcgtgctga tcttcagcta tgtcggtctc ccagcggagt tcgccatggg ccgctgcgcc
     2881 gccaccggca cgctcggcgc ttacgaaaac gcctgggcca cccgcggcaa aggggcaggg
     2941 aagacgggcg cggtgctcgg ctggctgccg ctcgcgggct cgctctgcat cgcgatcgga
     3001 tacgccgtta tcgtgactta tgttctcaag gcactcgtgg attccgttgc cggtacgctt
     3061 atgaccgcgg atgccgcggc gtggttttcg gacttttcct cgcgggactt ttccgtgatc
     3121 ccgtatcata tcattgtcgt cgccggaacg cttttgacgc tcttcctcgg cgcgcacagc
     3181 atcgaaaaga cgaaccggat catgatgccg gtgtttttcc tcatctttgt cgtcctcgcg
     3241 gtgcgcgtgg cgtttctgcc gggcgcggcg gagggataca agttcatgtt cacgccgcgc
     3301 tgggagatgc tctgcgaccc gatgatctgg atcnnnnnnn nnngggcgat gggacaggcg
     3361 tttttctccc tgtccgtcac gggcagcggc atgatcgtgt acggcgcgta cctctcgaaa
     3421 gaagaggacg ttgtcggcgt ggcgcagcac acggcgctct ttgacacgat tgccgccgtg
     3481 gtcgcggcgc tcgtcatcat ccccgcgtgc ttctcctacg ggctggacgt cggcgcgggc
     3541 ccgagcctcc tttttgtgac gctcccgacc attttgcagg atattccgct cggccgtctt
     3601 tttgcgatca ttctctatgt cgccatgatc ttcgcgggcg tcagctcgct gcagaacatg
     3661 tttgaagcgg tggcggaatc gctgctgcac aaattcccga agctcagccg caaggcggtg
     3721 ctcgcgctgc tcggcgtcat ttgcctcggc tgcggcatcg gcatggagac gatctccaaa
     3781 tggggcccgt ggatggatct cgtgtccatt tacatcatcc ccatcggtgc gacgcttggc
     3841 gcggtctcgt ggttctatgt gatgaaaaag gacgagcttc tctccgccat caacaccgga
     3901 gcgggccgcg agcgcggcgc agtctggtac gccgtcgggc ggtatgtgta tgttcccctt
     3961 gccgtgatcc tctgctgcgt ggcgctcttc cgccatgtgg cgttctgatg cggccttttt
     4021 tgtaaataaa tggggacttt cttgtaaatt ttggtgtatt tctcaaaaac acttgcgcgt
     4081 aacaagtata tagattatac taaactcaaa agagaagaac tctgcacggc gacttacgcc
     4141 gaaaaaacaa ttgaaaatgt cggcaaatgg caaatgttat attttttata tataataatt
     4201 tgcggaatag ggtgcaattc cctgcgagtc ggtcactgtg atgccgtccg aagtaccgga
     4261 acggataagt cagatacgca aagcccgttt gccgagacac ctgcctgtac cgggtctgtc
     4321 tcacacatca cagagggatt tctccatata tgtctgtttt gcgtctgtac ggcttcggcg
     4381 gtacggacgc ttttgtttta ttcggcagtg ccggcccgcg cgaaaaaaaa taaaaaggag
     4441 aaatgagaat gaagaaaaaa acacagctcc tcagcctgct tctggttttg acaatgcttc
     4501 tggcattcgt tccggccagc gcgcaggcgg tggacgagac gccgtatgat ctccgtgttc
     4561 tgacctttga ggacgcggat tataagggcg gcgcaaactt tgccggcggc agcgactgga
     4621 cttcgctgat cgatgatccg cagtacggcg gcccgctgct ttacggcagt agcggcgcgg
     4681 gtgttaccac ggaaagcgaa gcatataagt ggaccgatga aaataacact atgctttcac
     4741 acgtttttcc gtataactat aatgcatact gctattgggg cggtggacat gccatttcca
     4801 actatggcac cggagacatt gccgattacg gcggttatga agcgcagctt accgtatata
     4861 aaaagggcgt ttcgggactg acgcgcaccg gcgccggaca taacggctcg gataactttg
     4921 ccgtgcatta tggctatatc gatggcagcc aatataataa aaccgaagtt ctgccagctt
     4981 tttcctttac cgacggcgtt gagcgtgtgg ttgaccacat gtatatcacc aatacgacgt
     5041 acgcgctcaa ttgctacatc gacggtaatg gcctcaccgc aaagatcgga gacaatgact
     5101 gggtcaaggt cgttgcaatc ggctacgacg ccaacggcaa aaagaccggc gaaacggaca
     5161 tccacctctg caacgggccg gacaatatcg taatggactg gacaaagtgg gatctctccg
     5221 tactcggaaa agttcacaag gtcgcgttca acgttaccgg ttcgagtgac aacggctacg
     5281 gtttctccca gccggcgtat tttgcctatg acgacgtggc ggtgcgcttt gaaaagccgg
     5341 tttctgcgac cggcatcaaa ataagctcgt cgaagctgga gctttctgtc ggagagaccg
     5401 cttcgctgac cgcgacgctg acaccggcga atacgacaga agctgttgtg tggacaagct
     5461 caaacgatga tgt
//
LOCUS       DAWAAE010000138         5373 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6314_length_5373_cov_3.744829, whole genome shotgun sequence.
ACCESSION   DAWAAE010000138 DAWAAE010000000
VERSION     DAWAAE010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6314_length_5373_cov_3.744829"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1082 a         1610 c         1592 g         1089 t
ORIGIN      
        1 agcctgtcga gaaacccggc tgccgcttaa tgcccagccg ggtttcttga cagattggag
       61 cctcccctga acttgttccg gggaggctcg ttttgatttg cgttgatttt tttccgtatg
      121 ctgtcagtcg tccgcgccga gggcgtcgag ccccttgacg tggtctacct ttttcgcctt
      181 gatgttcttc acgagcagtg tgaacaccag gaagctcgcc gcggtgagga tggaggcgta
      241 gaccggcagc gtgcgccacg ccatcgtcgc gtccagcacg aagccgagag cgacgggcgt
      301 gatcgtctgc gctgacatgc tcgcggcgta gtaatagccg gtgaagcggc cgatcttctt
      361 gctcgagcac agctccacga ccatcgggaa cgagcagttg tgcacgagcg ccatgccgaa
      421 gcccttgatc gcccagacgg cgaacagcag cgtcgggaac gcaaactcgc cgcgcgcgcc
      481 gatgaccttt ccggtgggcg tggcaaagca catgatgata aggccgacga gcgtgcacgc
      541 aaggccgcag gagatcgtcc acttgcggcc gatcttctcg gcgatcgatc cggcggtggc
      601 aaagccgatg acggaggcga gtccgccgag gatcgtgagg atcattgtgg cgctcgaaac
      661 gctgttgaga tagtaaatga catagtttcc gatgtacgtg ccgagcgcgt tatcggacat
      721 gaaccagagg aactccgcgc cgaggatcgc aagcagcatg cgcttattgg ccttgctcat
      781 cggcttatcg tcgtcgatgg gcgtttcgac cgcggccatc tcctcgccga gagcgagctc
      841 ttccgccatc tcccgctcga tcttgttttc cttgatcgtg gcaaacagca caagcgcgga
      901 gatcaccatc agcaccgagg cgatgatgaa cggcagctcg atgacccaga gattgcgctc
      961 ggcgtcgggc gcgttgatgt agctgctcag cacaaggaac aggccgagca ccgtggcgaa
     1021 cgcgccgccg atgtagccca tgatgttgat gatgccgttg gccttggcgc gcaggggctt
     1081 gggcgtgatg tccggcatga gagcgacggc ggggttgcgg tacatcatca taaagatgag
     1141 aacgatggcc atcatcgcga ccatcccggc aatgttgtta ttgtggaaga acagcgggat
     1201 aaacgggaac gcgaccgccg aaacgaacgt gccggcaagg atatacggca tgcgcttgcc
     1261 gatcttcgtg tgcgtcctgt ccgaaagacg gccgaagagc ggcagaagga tgagcgcggc
     1321 aagattgtcg cacgccatga tgatgccgac gatccactgc acatgcagga tgtggttcgg
     1381 gtcgctcgcc ttgagatcgg cggacgacat gccgtagaac cggcgggcga agatatccgt
     1441 cagaaatgtg gggcaccagg agtcgtacac ctgccagagc aggagaatac caaaaaaggc
     1501 aaagccgatg agaaaggtcc gcttgtaatt gagcttcact gctgtttcct ctccgcgcgg
     1561 aacgcgccgc gcgcctctct tttatgctcc ctccatcata caggaaaaac cctctcttta
     1621 caacccgttt ttcctgcgtt acaataccgc tatgaggtga taccaatgaa acaatgcaaa
     1681 aaagccggaa cgatcgccgc ggccgccatc ggcgccgctg ccgccggata tctttttctg
     1741 atcgcgccgg gccgctcccg cgcgggacag cgcttcccct tccgcgggca gaacatcgcc
     1801 caccgcgggc tgtacgacgc tgcgcagggc attcccgaaa actctctcgc ggcgttccgc
     1861 gccgcggcgg agggcggata cggtgcggag ctcgacaccc gccttacgaa ggacggcgtc
     1921 gttgtcgttt cgcacgacag caacatccgc cgcatgaccg gcgccgatgt gacggtcgaa
     1981 tcgctcgact atgccgagct gcagcaatat aagctcggcg gcaccgagga gcgcgtgccg
     2041 cgcttttcgg acgcgctcga cattctcgcc gccgcgcacg tgcccgtgat cgtggaggtc
     2101 aaggcctcgc ccgaccatcg ccgcggcgag ctctgccgca aggtgctcga gatcatcgac
     2161 gcgcacaccg gcaatttctg cgtggagagc ttcgatccgt tcatcgtggc gtggttccgc
     2221 ttccatgcgc ccgacctgct gcgcggtcag ctcacggccc agcgggacga tctcggcggc
     2281 aatctcggcg cgtggctttc gagccggatg ctcatcggct gcatcggccg gccgcagttc
     2341 atcgcccacc gcacgggcaa aaagtctctc ggcgtgaagc tcgcggagct gctcggcgcg
     2401 atgcgcgtca catggacggc gcacgaccgc agcgaagagg ccaagtgcga caccgtgatc
     2461 tttgaggcgt accgcccgcc ggtacagtat aaatgagagc gctccgccgc gtcctcgcgc
     2521 tcgtgctcgc gctctgctgt cttggctttc tccccgttcg tgccgccgat gagaatgtac
     2581 ctgccgcgga gaccggctgc gcgcatcccg gctggcagaa cggcatatgc gtcgcgtgcg
     2641 gcgcagcctg cccgcacgcc gagcacgact acgattcctg tctctgcttt gtctgcggca
     2701 tgaccgtccg gcactcgtat ctcacgtccc gctgcccgat gtgcggccgg acgcccgctt
     2761 ttctcgactc gcgcgtgccg acgtccttct ttcaaagctc cggccgcccc ggcacggtgc
     2821 agtcggtcga atacctgacg cacgactacg tcggcgagcg caagggcacc ggcacgctct
     2881 cctattataa gcggatgctc gtctatctcc cctatgggta cgacgcctcg cagccgtaca
     2941 acgtgctcgt gctgctgcac ggcatgggcg gcggcgaagg atattggctc cggcaggagc
     3001 agctttacgc cgcttacggc accgactact acgtcaccac ccgcccgatg ctcgacaata
     3061 tgttcgccgc gggaatgtgc cggaacatga tcgtcgtgac gccgacgttc taccgcgatt
     3121 ccggcaactt cttccggtac gaccgcgtgc cggacgagga gcagttcgtc cgtgagctgc
     3181 gcgaggacat tctgccgttc atcgtgcaga actactcgac ctatgccgcc gacggcagcg
     3241 ctgaggcgat cagcgccgcg cgcgaccact ttgcctacgc gggtctttcg atgggctcga
     3301 tctacgccta taactccgtg atgccgctct gtctcgatct gttcgcgtgg tttggctgct
     3361 tctccgggag cgactgctac gtcgatctcg ccgcggacgc actttccggc gaagccaacg
     3421 ccgcttaccc gatccggtac ttttataaca gcatcggtac gcgtgactcc atggcaacgc
     3481 tccaccgcga aaacttcatc gctctcaccg accgcgtgcc cggcctgacc gacggcgaaa
     3541 acgcctggtt cacccagctc cccggcatcg gccacgaata cagcgcctgg gtcgtcgggc
     3601 tctataactt tttgcaggtc gtatttgcga tgtgatctca cacgcccctc ttcgccgcat
     3661 tgacccgcat aaacgaaaag atccggaagg acaacgtcct tccggatcaa ttttattctg
     3721 agaattattc gttcttctcc ttctccgtct tcgcgttatt ttctgcgctc tcgcccttca
     3781 cggccgaggc agcgagcgtg cgcagcccgc gcttcgtcgt gagacggcgg taggcctgcg
     3841 gcgacatgtt gtgcgtagac ttgaacgtgc gggagaagtg atccacgctg tcgtagccga
     3901 cgagcgcgga gatatcatgg atgcggagcg tggaggtcga gaggtattcc tcggcgcggg
     3961 tcatcttgat gccgcggacg atctccgtaa agctcatgcc catgtagttc tgcagcatac
     4021 gggagagata ggtctcgtta taatggaacg ctctcgccag cgtgctcagg cgcacggtac
     4081 ggtagttctg ctggatgtac tgcaaaattg cgccgcagtc ggcacgggtc gattcatcgc
     4141 gcgcggcgcg aagcacagcg gcgctcccgg cctgtgtgcg gaacacggtc gcaaggaaga
     4201 tctttgtcca gcacacggag gtgagattcg cgaacggcgc cttggacgcg cactccttga
     4261 cgagctcgtg cagcgtagac agcacgtttc ccgactcaaa gttcgccgtc atgctcatat
     4321 aattcatttc attctcgccg tacatcgact cgcggaagaa aatgctcatt ttgtcgctgc
     4381 ttgtcatgag atcgccgaac tgcgcatcga acgtggagcg gcgcatatgc agcgctatga
     4441 cgtacgcgtc cgacgggacg gccttgatgt gcgccgtgtg cggaggaacg atgataacgc
     4501 tgccgacgcc gacggggatg ggcgttccgg caaagaagaa ctcgcactcg cccttgagaa
     4561 cgacggcgat cgtaaagtag tcatactccg cggcatccgc gctcaggtag gagaaaacac
     4621 gcgtcacggt aacatcgcgg ccgggcgcaa agactgcgtc ctcaccggaa acatgagctg
     4681 tgccggactc cgacggagag gtgttctgaa ggaacgcggt ggcattcacc ggctgagaat
     4741 cgatgatatc acagatctct tccacggtat gctggacctt ggaatcggcc gcggcaacag
     4801 cacgcggagc ggaaaggaga ttgtcctttt ccatcatatc aagcgcctcg ccaagggtgg
     4861 ggcgtacgcc tgtcttgcgg taataggcag agagaaactc ctcgagctgc tttaccgttg
     4921 tttgtaaatt atttttttcc atggatgatt cctccaattg ggaaaagggg ttagatgccg
     4981 tacaaaataa tacacatgta caaatatttg caaacatact atacaattaa agtatggaaa
     5041 ccgtcaagac ccttcgtaaa aaagccgcag agttttccgg gaaaattcgg cggattgata
     5101 acttaagaaa aaaattacag gatggcataa aagcgcgccg ttaattttgg aaataataaa
     5161 aatacggaga gatgcatttt atcatctctc cgtaatcttt gtgttggcgt tgacctattt
     5221 tcccggcccg tcgccaggca agtatcgtcg gcacaggtga gcttaacttc cgtgttcgga
     5281 atgggaacgg gtggaccctc accgctaaaa acaccaacta tggtacacct tcagggactc
     5341 gaaccctgga caccctgatt aagagtcagg tgc
//
LOCUS       DAWAAE010000139         5308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6397_length_5308_cov_2.532838, whole genome shotgun sequence.
ACCESSION   DAWAAE010000139 DAWAAE010000000
VERSION     DAWAAE010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6397_length_5308_cov_2.532838"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1107 a         1527 c         1551 g         1123 t
ORIGIN      
        1 actgccgcac atcagatttc acgacctcag acataccttc gcaacgctgg cactgcaaaa
       61 tggcgtggac gtcaaaaccg tcagcagtat gctcggccac tatgatgccg gattcacact
      121 ccgcacctac acccacgcca cacggcagat gcagcaaaag gccgcggaga aaatgggcag
      181 tttcatggcg cagattcgat aagtcccaca ggcaagcaga gcaccggaaa ggaaacggtt
      241 tcctctccgg tgctctttgg ctctttccgc acagttcgtt gtgtgggtca cggtgtgggt
      301 cgggccggtg tttccagacg gtaatttttg aaaagcgtcg aagaaaaatg ccttcggcgt
      361 gaggtgtaac cgcaatttgt gtgggtcaaa acttttctta aaagttatga aaacagcgga
      421 aatcattcga tttccgctgt tttctggagc taatgatgtg actcgaacac acgacctgct
      481 gattacgaat cagctgctct accgactgag ctatgccagc agcttctgcc gatttcttgg
      541 cagcctgtgt attttatcac gtcgcgctgc cgttgtcaac agaaattgcc ggtattttgc
      601 gcccggtctg cggctttttc gcttttcctc cggtaaactg tcccgctggt gtgggcggag
      661 cagagccccg cccctacagg ggataaaagc ggtcaagctc ccattttttc ggattgttgt
      721 cgatgtactg ccatacgtcc cgaaaatcat cttcatttcg tactacatga tcgtaaaatg
      781 accgctgcca taccacgttt cccggcgtat gcatttctgc attaaccaat ttggtcgtga
      841 cggatttcca cgagcatagg atatcagata ttgtaggggc ggggctctgc tccgcccaat
      901 aaagagagag cagcatatga acgtgattcg gcatgatcac atgacgttca atttttatgg
      961 gataacgttc ctccagaagc aaaagctgcg tttcacaaat gcggccgaga gctgaaagaa
     1021 tcgtacatcc ttcgtggatg gctgaaagga tttgccgccg gcttttggtg cataccgtca
     1081 gaaaataagc gccgggcagg gaatagtcaa actcacgcag ccgcatatca cggcgctttt
     1141 tttcgttcac tcagcagctt tgacgctcac catggcgtca aaatcgagtc cgagctcgcc
     1201 gccgacggtg cgaagctccg cgagcgtgga gtcgttgacg gggatgccgt ttttgagctt
     1261 ttcattgcgg gaatcgacct ccatctcgcc ggaggtgtaa atgcgggtgc ggccttcggc
     1321 cttggcgctg tcgcgcagct cctgcagaag cgtcgaaagg ctctcgcgca tcgcggcctt
     1381 gtcgccgaac atgccgtaat cgagcgcgaa gaagctctgc gacgtgtcac cgaggccgtt
     1441 cttgctcaca tggttggacg tagcgccgcc ggcgatgatc gaggagagga tttctacgat
     1501 cagcgcgaga ccgtagccct tgtgcgagcc ggtcgcgggg ctggagccgc cgagcgggag
     1561 aatgccgccg cccgccttgg agttgatgtt gtcgatgacg cgcttggcgt cggaggtatc
     1621 cttaccgttt tcatcgacgg cccagccgag cggcatgggc ttgccctgct tggcatagac
     1681 ctccagcttg ccgcgcggca cgaccgtagt cgccacgtcg tacagcatgg gaatgggatc
     1741 ggcgggcatg gcaaaggcga tggggtttgt gccgagcatg acctcgcggc cgaacgtcgg
     1801 cacgcagatg gcctgcgtat tcgtaaagca catgccgagc atgtcctcac gctcggccat
     1861 catggcataa tagccgccga cgccgaagtg gttggagttg cgcacgaccg ccgcgccgaa
     1921 accgtactgc ttcgccttgg cgatggcgcg ctccatcgcg tccacggcgg cgatctggcc
     1981 catggcgctg tgcgcgtcga gcacaacgga gagcggcgtt tcatgcacga cctccggcac
     2041 ggcgtccggg cggatcacgc cgcttttcac accgcggtgg tagcggatca gacgctgcac
     2101 gccgtgggat tccagtccct gcagatcggc ggtgagcaga atgtcggcaa tatcgcggct
     2161 ctgtttttcg ttgaatccgt atttgctcat aacggcttcg cagtaagcgc gaagctcctt
     2221 ttccggcaga cgatagtacg ccatatgtct ctttcctttc tttatcgaac tggatcaaac
     2281 cttttcgacc gattatacca tttataccat attcggacat aggaaaaaag ccttttccgg
     2341 cggaaaaaga ccgcacacgg tattctgcaa aaagacattg gaaaatctcc gggcaggacg
     2401 tataattatg gcagaacgtt gagcgggagg aacggacgat ggataaatac gaggtgctga
     2461 agcactgctt cggccacagc cgtttccggc aggggcagga ggagcttgtc gacgccgttt
     2521 tgtccggccg ggatgcgctc ggcatcatgc cgaccggcgg cgggaaatcc ctgtgctacc
     2581 agatccccgc gctgcttctc ggcggcatta cgatcgtcat atcgccgctg atctccctga
     2641 tgaaggatca ggtggcggcg ctcaaaaacg cggggatcag cgccgcgtac atcaacagct
     2701 cgctcaccgc cgagcagctg cgtcttgtgt accgccgcgc gcgggagggc gcgtacaagc
     2761 tgctgtatgt cgcgccggaa cggctggaaa cggagggctt cgccgcactc atgcaggcgg
     2821 tgacggtctc gcttgtggcc gtggacgagg cgcactgcat ctcccaatgg gggcaggact
     2881 tccgtccaag ctaccggagc ataccggact ttgttgcctc gctgccgcag cgcccgccgg
     2941 tgacggcgct caccgcgacc gccacggcgg aggtgcagca ggacattgtc cgtctgcttg
     3001 aactgcatga ccccgtccgc tgtgtcacgg gcttcgaccg cccgaacctc ttctttgacg
     3061 tgcagcgccc gaaaaacaaa atgtccgcgc tgctcgatct tctgcggacg cggcgggaca
     3121 agacaggcat cgtttactgc gccacgcgcg ccgccgccga gcgcgtttgc cggacgctgt
     3181 gcagccgcgg cgtcgccgcg gtctgttatc acgccggaat ggaggacggc gagcgccgcg
     3241 cgagccagga cgattttcag ttcgaccgga aaaacgtgat cgttgcgacg aatgccttcg
     3301 gcatgggcat cgataaatcc aatgtgaatt tcgtgatcca ctacaacatg ccgaaaagcc
     3361 tcgaagcgta ttatcaggag gcggggcgcg ccggacgcga cggcgaggct gcagactgca
     3421 ttttgctcta ctccgcgggc gatgtggaga cggcaaaatt cctcatccag agcggcgggg
     3481 aggagcttgg cgacgcagag cgggaagagc tgcgccgccg cgactatgag cggctgaaga
     3541 tcatgaccgg ctactgcaaa acgacgcgct gcctgcgagg atacattctc gactatttcg
     3601 ggcagccgca cgccgaggaa tgcggaaact gcggaaactg ccggagaacg taccggcttg
     3661 aggacatcac gataccggcg cagatgatcc tctcgtgcgt tgtgcgcgtg cgcgaaacgc
     3721 tcggatacta tgtcggaaaa acgctgatcg tccagacgct gcgcggcagc cgcgaccggc
     3781 gtcttctgca gctcgggctg gacaggctct caacgtatgg cctgatgcag aagctctccg
     3841 tggaaaatgt gctcgccatg ctggaccggc tggaagaagc ggggtatctg cgcacgaacg
     3901 cgcagcactt cacgctggaa ccgaccgcgg ccgccaacgg cgtgctgttc ggcggcgaga
     3961 agatcgccat gccggtacac cttgagccgg cggcgcagag cggagagaaa aaacgccgcc
     4021 ggaagagcgc ggcggcatcc gccccgatga ctggcacgga cggtctcttc gcggcgctca
     4081 aggcggagcg cacgcgcctc gccgcggcgg agggcgtgcc tgcctatgtt gtattctcca
     4141 acgcgacgct cgcggacatg gcggcgaaag ctccgcacac gatggaggat tttctggagg
     4201 tctccggcgt cggcgagata aaagccgcgc gctacggcga agcgttcctg aaagccatcg
     4261 ccgcatacga agaagcggac gaagcctgaa aaaaaaggaa agccctccgg catccgtccg
     4321 gctctataag gccggacgga tgcttaagac ttttggcgat tcataaatcc aaaagctcaa
     4381 tatcgtgcgt cagcacatcg gtatattgca gcgcatggcg cttcggcggc ccggacgaga
     4441 cttcctctac ctgaaagatg tgccggtaga cgcccgtgat cttcaccgga acattgttca
     4501 aatgcagcct tggctgccgg agagaaacat tgatgcgaat ggttggatcg ctcaggtaac
     4561 gctccgaaat gcgctgccga atctgctgaa gctcataaat atcattcatc atacccacgc
     4621 tttctatcac aaacccgcct gataaaagtc aggcgggttt ggcgttagtt gaagtattcg
     4681 cttacatcaa ctttgtcgtt atactgcttg cggacgcgaa ccagcacaga gccgtcggcc
     4741 tgctccgttt cctcgacgat ctgataccgg gtgcggtttc tgtccatcgt cgccttgtag
     4801 tggttcactt cgctgcggtt gatcttgagc tgctgctcac gggtcagacc gcaatcaggc
     4861 ttctgggaaa acaccagcgt ttgcaggatg cacgctgcct ttacgcgctt cattgatttc
     4921 acctccctct gcctccgccc ggtcagaacg gatgctcttt ttcagagaga tgatcagtga
     4981 acaaacagcc tgtgcacgtc agtctgccgc caacgcccac cgcaccggat tcactcccgt
     5041 gataccttga gtatatttat attttaacga gtttcaaaac gaatttcaaa cgttttttct
     5101 ggaaagtttt caatacagct ccgcgatgat gtccacgcac ttgtcaatcc ccagcacgcc
     5161 gctgtccagc gtcaggtgat aattttgagc gtgtccccac ttcatattgg tgtagaagcg
     5221 gtggtaggca gaccggcgtt tatccttatc cctcaggcgc tcctccgggg actgctcccg
     5281 ctcgccgtag acctccacga tgcgcttg
//
LOCUS       DAWAAE010000140         5295 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6419_length_5295_cov_3.543511, whole genome shotgun sequence.
ACCESSION   DAWAAE010000140 DAWAAE010000000
VERSION     DAWAAE010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6419_length_5295_cov_3.543511"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1061 a         1611 c         1579 g         1044 t
ORIGIN      
        1 cttcgaccag aacctcgcgc tgacgggggg gcggccgttc ctgcagcagc cggacggcag
       61 cgttataaag acgacggaaa tgctcgcgct gaacatttac agcgccttct actccggcgg
      121 cggcaacaac cgcggcgtcg gccaggcgaa ggccgtggtg ttcttcatcc ttgtggccgc
      181 gatcggcctt ctgcagctcc aggccacccg taaaaaggag gtacagcagt aatggcaaag
      241 agagaagaac gcggcaaaat ggtgctgacg atcatcttca cggtgatctg catcgcttac
      301 attatgccga tcctcatcgt tttgatgaac tccttcaaga gcaacgccgc cgtcaacacg
      361 cagacgtttg ccttcccgaa cgcgaacagc tttgtcggct tccagaacta tatcaagggc
      421 atgaccttcg gcaactaccc gtttctgaag gccgtgggct acagcctttt catcacgctc
      481 gcctccacgg cgctcattct cgtgtgcacg tccatggccg cgtggtatgt ctcccgcgtc
      541 gggagcttct tcagcaagat cgtatattat ctctgcgtgt tctccatggt cgtcccgttc
      601 cagatggtca tgttcacgct ctcccgcacg gcgaaccagc tcagtctcga taccccgtgg
      661 acgattccgg tcatttatct cggcttcggc gcgggtctcg cggtgttcat gttctgcggc
      721 tttgtaaagt ccattcctct ggaaattgag gaagccgctg ccatcgacgg ctgcggcccg
      781 gtgcgcacgt tcttccaggt ggtcctcccc atgatgaagc cgacgatgat ctccgtcggc
      841 gtgctggaga tcatgtgggt gtggaacgac ttccttctgc cgtatctcgt gctcgaccgc
      901 acggagtata tgaccatccc catcgtcata cagcatctca agggaagcta cggacaggtg
      961 gacatgggcg cgacgatggc gctgatcctt ctgagcatcc tgccggtcat catcttctat
     1021 ctctgctgcc agaaacacat tattaagggt gttgcggccg gcgcggtgaa gggctgatga
     1081 cgatcaagga cctggcggct ctctccgggt attcgctcgg cacggtttcg cgcgtgctca
     1141 acaatcagcc gcacgtcagc gaaaaggcaa gagagcagat catggcgatc gtgaaggaac
     1201 agggctttga gctgaacacg aacgcccgca atctcaagca gacccacagc aacaacatcc
     1261 tcgtcatcgt ccgcggcacg cacaacgagc tgttcgccgc gctggtagaa cggctgcaga
     1321 ccgtggcaac aggcggggag taccagcttg tggtggacta tatcggcgag ctcgacaacg
     1381 aggtgcgccg cgccgtgaag cgagtgcgcg aggtaaagcc gcagggcatc ctcttcctcg
     1441 gcggcagcag cgaggatttc atcgaatcct ttgccgagat ccacattccc gcggttcttg
     1501 tcacaaactc cgccgcggaa ttccaattca agaatctttc gagcgtcacg accgacgata
     1561 ccgccgctgc cgcggcggcc gtcgggtatc tggcggacat gggtcataaa aagatcgccg
     1621 tcatcggcgg cgagcgtgaa tactccgata cgggccgtct gcgctatgcg ggcgtgctgc
     1681 gcgcgctcac ggagcgcggc ataacgtttg acgcggaccg gtattataag accgcgcact
     1741 acagcttcga cgagggctat acggccatgg cggagctcct gcgtgaaaca tcggagatca
     1801 cagcggtgtt cgccatggcg gacgtcatgg ccatcggcgc ggcccgcgcc gtccgcgatg
     1861 ccggaaagcg tatcccggag gacatttccc tcatcggctt tgacggcctg cccatctgcc
     1921 ggtactacga gccgaggctc tccaccgtta cgcagcagat cgcgttcatg gcggaaaaga
     1981 gctacgccgt gctgctcgac atgatcgaaa acggctcggg cgcgcagcat gtccttgcgc
     2041 cgtacacgct cgaaaagacg gacagcatcc gtaaaaatta acagcatccg caaattctaa
     2101 cagacgaaag aggcgtttgc catgcgagca agcggaatca ttctgcatat cacatcgctc
     2161 ccgtcgccgc acggcgtcgg aacgctgggc agagaggcga gagagtttgc cgatttcctc
     2221 cggaaagccg gacagaaata ctggcagatc ctgccgctcg gcccgaccgg ctacggcgac
     2281 tcaccgtacc agaccgactc ggcctttgcc gggaacccgt atctcatcga ccttgagcag
     2341 cttattaccg atggtctcct cacgcgcgag gaagtcgatg ccgtttcctt cggcagcgac
     2401 ccggcgcaga cggattacgg cgcgctctac gccggacggt tcgatcttct ccgccgcgcc
     2461 tgtgagcgcg gctgggacaa agagcgtgag aacgtagacg ctttctatcg tgaaaacgag
     2521 agttggctgc gcagctatgc gctctatacc gcggcaaagc gtcatttcgg catgcgcccc
     2581 tggatcgaat gggaggacga ggatctccgt cttcgccgca gcgaggaagt gctgcgccgg
     2641 tatgaggagc ttctcgcgga ggatatccgc ttctgcattt tcacgcagta tctgttcttc
     2701 cgccagtgga acgcgctgcg cgcgtatatc cgcgagcagg gcatccggat catcggcgac
     2761 gtgccgatct atgttccgct cgacagcgcc gacgtgtggg cggagggcga gtacttccag
     2821 ctcgacgatc agtgccgccc gacggaggtc gcgggcgttc cgcccgatta cttcaccgcc
     2881 gacggccagc tctggggcaa cccgctctat gactgggacc gtatgcgaga cgacggctac
     2941 cgctggtgga tccggcgtct cggcgctgcg ggcaagctct acgataccgt ccgcatcgac
     3001 catttccgcg gcctggagag ctattgggcc gtgccctacg gcgagacgac cgcgcgcagc
     3061 ggccattggc gcaagggccc cggcgagagc tttatcgacg cgatcaagac ggcgctccct
     3121 ggtctcgaca tcatcgccga ggatctcggc tttatgaccc cggaggtcat cgccctgcgc
     3181 gagtattcgg gcttccccgg catgaaggtg ctgcagttcg cctttgattc ccgcgagccg
     3241 agcgactatc tgccgcaccg ctacccgaca aattgcatct gctacaccgg cacgcacgac
     3301 aacgccacgc tcgcccagtg gctgggcgac gcgaagggcg acgatgtggc ttacgccaag
     3361 cagtatctcg gcttgaacga tgaggaaggg tatgtaaacg gcatcatccg cggcggcatg
     3421 agctcggtgg caaagctctt cgtcgcgcag atgcaggact ggcttgccct cggcggcgag
     3481 tcgcgcatga acgtccccgg cgtcctcggc cacggcaact ggtgctggcg catgctgccc
     3541 ggcgcgctca ccgacgagct cgccgaccac atcgccgcta tgacggccat gtacagccgg
     3601 taatatagaa caaatgccgc tctccggcct cattcggaga gcggtttttc attttttccc
     3661 atattctttg taagcttttt gccccgaatt gcgtctataa gggtgagtac tcaaggggca
     3721 gccccgtaag aaagaggaaa gatcatggag gacagcttca tcgtagacct gtactggcgg
     3781 cgcgacgagc aggcgatcgc cgaaacggag cggaaatacg gcggcgcatg cgaaggcatc
     3841 gcgcgccggt ttctccccgc ggcggaggac gccgaggaga tcgtgcagga cgcttatctc
     3901 tcactctgga acaccatccc gccgcagcgc ccgaagcatc tcggcgcgta tctgttccgt
     3961 accgtacgga acgccgcctt ttcccgcttt cgcgccgaca gcgcgcagaa acgcggcggc
     4021 ggtgaggcgt ccgccgcgct cgacgagctc gctgagtgcg tcgcggggcg ggacaatgtg
     4081 gagcagatca ttgaggcacg ggagctttct catgcggtga acgcctttct ccatgcgctt
     4141 tcccgaaacg accggatcat tttcaccgcc cgctactatc tcgctcttcc cgccccggcg
     4201 atcgccgaaa ggctcggctg ccgggaggga agggtacata cctcgctttg ccgcagccga
     4261 aaaaagctgc gcgactatct cattaaggag gaactgctgt gaaagcgctg gatattatgg
     4321 aagcggccgg tgggatcgac gatgaaattc ttatggaggt cacatcggct atgcaggaaa
     4381 agaatacagg aaaacccctt cgcgcgaagc ggacgcttcg caccgtgctg atcgcggcgg
     4441 cgctcgtgct cgtgctcgcc gggagcgccc ttgccgccgg gcggcttatc aacagtcccg
     4501 cgcaggcggt caaggtcgcg cggcaggaaa ttgcaaaaat gcaggatatg ggtattctct
     4561 cttcggaatt ccgctttttt gacggcgaac cggactatct cggcgaagcg ccgaacatgg
     4621 gcgtgagcga ctactggttc ggacgcattt tcccccacag ctacattgtc cgcggcggga
     4681 ccgacgacgg gaagtattac atgaatattt tcgtcgatac aatgagcgga aagatcgtgc
     4741 agctcactac ggaggccaag gcggacgaga cggacacgcc catccccggc ggcgatgtaa
     4801 cggtggaaac gggcaacgga aaggagacgt tccataccta caataactat gacgacatcc
     4861 tgccggacgg catgaccgtg gataagttct gcacgctgct cgcaaaatac tggggctttt
     4921 ccggctaccg tctcgcacag acggacgatg ccatgttcca ctatgcccag ctcaatccgg
     4981 gcggcggcga gcggctgacg gattttgccg gcgcgaacag ctacctgacc gtatatttcg
     5041 acggcgacca gagcggcacg ccgatgtaca tcagccttga ccggttcccc ggacgggtga
     5101 gtgtgctctt cggcatgctc caccaggtcg gctgatgcgg tgatcgttcc gctgttctgc
     5161 ctgcttcttt cgctgtttct cctcttgata gagcaataaa aatacccccg ctcttcggct
     5221 cgttccgaaa agcggggggc gagcttgtca aaaaagtatt tttgacaagc tctcaaacga
     5281 gacccacttc gttgg
//
LOCUS       DAWAAE010000141         5260 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6480_length_5260_cov_3.146013, whole genome shotgun sequence.
ACCESSION   DAWAAE010000141 DAWAAE010000000
VERSION     DAWAAE010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_5260_cov_3.146013"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          861 a         1742 c         1522 g         1135 t
ORIGIN      
        1 ggtcggggcg gtcgatcttg ttgacgacaa cgatcacgcg gtggccgagc tccagcgcgc
       61 ggcccagcac gaagcgcgtc tgcggcatgg ggccttcggc ggcgtccacg agcaggataa
      121 cgccgttgac cattttgagc acgcgctcga cctcgccgcc gaagtcggcg tggcccggcg
      181 tatcgacgat gttgatcttg acgcccttgt aggtgacggc ggtattcttc gcgagaatgg
      241 tgatgccgcg ctcgcgctcg agatcgccgg agtccatcac acggtcgacg acctcctgat
      301 tttcgcggta cacgcccgac tgcttgagca tttcgtccac gagcgtcgtc ttgccgtggt
      361 caacgtgggc aatgattgcg atattgcgta attccataat atccatctcc aaatgtaaat
      421 ccgattcaaa accagcgaag tattatatca caatgcattc tatatgaaaa gagatttttc
      481 tcatttttcc attgtttttc aataaaaacg agctgtcaga aggcctcgcc agcgtttgcg
      541 cccgcaggcg caaaataaag ttttaaccat tttctccggc ggcgtgtacg tcggcaaaaa
      601 cccctttcgg ccccagaccc gccaagcaga tctcccgttg gacaatagaa aggaggatcg
      661 tccccgccct gttggggtcg atcctccgag gagaaaagta tgggaccttc cgggctgctg
      721 tgctgggcag ccggaggtcg gagagacggc ttttttattg aaagcccaac ttccccgcca
      781 ttatgatatt agcgcaatca tttccttctg tcaaggatta atttgaaaat taaatatcac
      841 tctcccccga agcgcggagc agcttccaga tctcctccgc ctcgtcgctc cggatggcgc
      901 cggaggcgag ctgcgcggcg atatactcca cggcggcgtc cgcgccgcgc tcggctttga
      961 tgagaagcga ctcggaaagc gtgtcggcaa ggcttgagcc ggagccggag tttccggcat
     1021 ttccggcgtt ccgcgcgcgg cgcagcgacg caagagcgcc gttccgcccc atccagatct
     1081 ccgcgagata gtccgcctgc tcctgcgtga gccccatgcc gacatagccg gagtaatcgc
     1141 ccgccgcggc gagcagcgcg gcagtcttgg cggcgccctc gcgccggtgc ttttccgcct
     1201 cctgccagag cttggcaaga ctctcgccgt gcgtcctttt cgcctcggcg tcagcgcggc
     1261 tctgctcggc tctcgccgcg gcgagccggg cgtcgcgcgc agaatatgtc ttcgcttcct
     1321 cggcaagacg cgctctctcg ttttcggcga gctcctcgcc gtaggaattg gcaaggcgca
     1381 cctgcgagga ttccgtcagg ccgccggtga tgccctgtgc ggcaagacgc tgcggcagcg
     1441 tgcgcttgct ctccatatac tcgcggtaaa gctgacggtc ggtgctctta tatccgccgc
     1501 ggatgcgctc gagcgcgtcc tgcgcgtccc gctcggccgt ctctgcggcg gcatcggcgg
     1561 cggttttatt ttcggcaagc gcatcggcgt accggtcgcc atagtagccc tgcatggttt
     1621 cgctgtacgt cgggcggcgt tcgggcggcg ttccggccgc tcccgtgtcc tttccgccgg
     1681 tctccccgct ttccgccggg gcgctgctcc agcccgtatt cgtcaggttt ttcttctttc
     1741 tgcgccattc ctccgcgctc ttgccgggcg gggtctttgt tccaaaattc tcaagtcgta
     1801 ctgccttcat tttttcttct ccttatcgta tttgatgcat ttcggattgc ggcacaccca
     1861 ttgcgccgcc gttttgcgca gcgtttcaat gccgcagcac ggacatttca taccggctcg
     1921 cctccttcag cgccgccctc ttcacgggag acgtatgcgg catagccgtt gacggaggga
     1981 accgtctccc ccgtcggcgc gaacctccgc tcccactccc cgacgatctc ctgccggtcg
     2041 ggaatgtcga gaatttcgag ctcggcggcg aagatgcgcc agttttccgc cgtgacgttc
     2101 gcccgtgtca gactgtcgag cgcctgcagc gtcgtctgcc ggtcacgccg cgcgccgtcg
     2161 gcggcctgca ccgtgatatc cacccgcggg aaaaattccc gggacgggcg caccacctcg
     2221 ccggtgagag agcggatttc cggcagcgtg cgggcaaagt cggcgctgtt gtagatcatg
     2281 cgctgtcccg gcgctctcat cccctccggc gcgccgagat acagcatccg gtcgtcgtcg
     2341 aaaaattcca ggcagagcca gtccagcagc tcatagagcc gttcaaaccc ggcgtttcgg
     2401 tcggcgcgct tgatgtccgc ctgctcgcgc ccgtcggcgc ggagcatcga gaggcccgag
     2461 gcggtcgtga cgcgcgccgt ctccttgccg gtgccggtgt cgtagctgcg gctggcgcgc
     2521 tcgatctgct ccttgaacca ggtgatatcc atcgccgcct tgccgacgct ctgcagtccg
     2581 ccgaggcgct gcacgccgcc catgcgtccc tgcttgagat gtacgatcgc gcccggctcg
     2641 ttcgtaagct cctcgccgtc ggccagggcg ccgtcctcca cgagcaggat gtcgttggag
     2701 agaaagctgt cgttcaaaag tgccgaggcg agcttgcggt ccgcggcgtc tacaagatcg
     2761 agcaccgcgg agagctcgct cttattccag aagcggttct cgtcctgaat gcgccagtag
     2821 tgcacgaacg gaaagagcgt gttctgcgcg cccgtgcgcc gccagtagtt cgggatatac
     2881 cgcagctcat gtccgcccgc ctgcacagag cacgccaccg ccccggcggg gatcgtctcg
     2941 ccgtccacgc tcgtttcgac cggctggcgg aaccagtgct cgagcacctg tacggtgtcg
     3001 tccgtgtcgc tcagcgcgct cgtcatgtcg aagatctcgc cgcggggcac ataatcctcg
     3061 ccgagcgctt cctccgccgt gatgccgagc tcctcgagat cggcgcggaa tacctgcgcg
     3121 aagcgcacct tgtggatgcg gtaaacgtag tcgagatact gtccgtcctg cacgctgccg
     3181 ccgcgcaccg cggggtcggg aaacaccgcc tccacgggga tatcgctcac gcggatatcg
     3241 ccctgcgcct cgccgcagcg catgtcctcg tcccagtacg ccttccagaa ggcgtcgccg
     3301 agcttcagaa ggcggcgctc gttgcgcgtg ttcatgtcgg aaaggcggtt gttctcggcg
     3361 atgtaccgca cggcaaattc gcgccgcttg gccatggcgc tgtcaagatc gtcgtcgcgc
     3421 ccgcgaaatt ccggctgcgg cacggtcggt tcgatctggc tctcgacgag gatatacgga
     3481 tcgggaatgc tcgccgggag ccacgggatg tcgttctcac ggcagaactc ggtgagatcg
     3541 cgcgtcacat cgtggatgcc gttgtagtaa tcgttgtact tttcccactc gatctccacg
     3601 gcggtgcggg cgtttttggc gcgccggaag agcgcgtacg ccgtccgctc gcgcgccgcg
     3661 cggtcggaat aatcgtagcc ggaaaggacc tccggctgcg gctgtttctt tcgtaatttc
     3721 atctcacacc cccagcatcc ggttcacttc gccctgcacg agatcgtaaa accacgcgcc
     3781 gagcttctgc tttcgctcct cgccgttgcc ccacttcccg tcaagcacct cctgcgccat
     3841 cgccgagacg cttacacatt ccccgtcctt ttcgtagggg cggggctctg ctccgcccgc
     3901 cgcgccatcg tcaaaatacg aaagcggcac atacaaaata tccagatcca gcggactgcc
     3961 acggtactgc tgcatgacgc acttcccgga aaggtcggga taatgcacgc cgtcgttcgc
     4021 gccccacgcg gcgatccagc ggtcgtaccc cgtctcgccg atgtgtgtat caaaccagct
     4081 caggctggcg tacacgccgg ttctgttccc cgcgtcttcc atagccgcgc agaacgtttt
     4141 gcacatcgcg ttgatggtct cattctccgg gaagccgttc tttgccttgt acccgtcggc
     4201 gtcctccatg tcaaaccaca cgccgagacg gggcctccgt ccggcaagaa acttgagaca
     4261 agcttccgcc tcctgccgtg cctgcgcctc gttgagcgcg taggaatacc agtaaagccc
     4321 ccacggaatg ccgagcttct cgcactttgc gatgttccgc tccgcccacg ggtccgcgct
     4381 cgtccagaac ccgccgcgga tgataacgaa gccgtcttta tagggcgtca ggtccatgtc
     4441 gccctgccac tgggaaatgt cgataccgtt catttccata tacctcctgc tttgaatttt
     4501 accggcgcat ttttcaatgc gccgcccttg cagcagagca tcgcctgctt ccacatcccg
     4561 ccggtcttga agtacagcac atcgccgacc ggctccggta cggcaagggt gaccgggctg
     4621 tttgccgtag tcggtctgtg cccgctggtc attccggcag tcaccgtcgc gccggtatac
     4681 gttgacggaa gcgtgtagta ataagtagcg gcaagataat agccgctgcc gtaattgtac
     4741 gcttcgctcg ggccgaactc gttttccgtg ccgtcgctgc cccaggggat gtacgccgct
     4801 ttgtttgccg ctccccatcc cataattgcg ttggagtaca tctttatccg cacacagata
     4861 gagttatcgg aaagccgcgt aacagcgcat ttggaatagt aggcatagcc gtacttcccc
     4921 tggttgtaca tgctaacaat ctcgccggat acttcctcgc tccatcccgt cacgttcgtt
     4981 ggctctgccg tgctgaacgt tcccattccg ttcacctcac tccgaataca tgagatagat
     5041 atccccgtcg ctgccgagct cggcgcccgg ctccgtcgtt ccggcgtaaa cgtgccgcac
     5101 ctgatcggcg gcaaggccga actttgtata cgggatatcg ttcgcgagct tctcggcggt
     5161 cacggcgttc ggcgcgagca gcgctgtgat gatagctccc gcggcgatct tctccgccgt
     5221 gactgcgccc ggtgcgatct tctgcgccgt caccgcgccc
//
LOCUS       DAWAAE010000142         5170 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6608_length_5170_cov_2.971848, whole genome shotgun sequence.
ACCESSION   DAWAAE010000142 DAWAAE010000000
VERSION     DAWAAE010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6608_length_5170_cov_2.971848"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2704..2713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3484..3493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          930 a         1536 c         1704 g          980 t
ORIGIN      
        1 gggaaaacat cgatctccag cgtgtgccct tcaaagggca caagatagcg cgtcttttcg
       61 atcgtgcgca gcgcctcgtc gcgctcggag aggagcgctt caaactccgc ggcagggatc
      121 tcgtgctcgc gctcaatgcg cgttacggcg gtgacgcgcg tcttttcggt atagtaatac
      181 cgcgtttcat cgccgcagcg ggcgcggcgg atgcggcggc ccgcgccgga ggtcccggcg
      241 cggagataga cctgcgcaat ggcaagacgc ttcacgctct tttccccgag cagcgccgcg
      301 tccggcagcg caatgagaaa tttccgctcg atctcaagcg ggagagtatt ttcgcccata
      361 accgttgtcc tcctgcataa aaaaggctcc cttgtgcaaa gggagctgtc agccgtaagg
      421 ctgactgagg gattgtcatt ttgagatcac gaggatcaac aactcctccg tcaaaacctt
      481 cggttttgcc atctcccctt acacagggga gacttttatt tagtatatca cagcttcgtc
      541 tgcaaatgca tcagcatctc ggaaagcgcg ccgcaggtcg cagtctcgtg tgcgccgatg
      601 gtgccgtcgc cgtgcgcggc gatcatgccg gactgcacgg cccagagcat cgcctgctgc
      661 gcccacgggc tgacggagct gccgtcgctc catgcggcga gatcgcccgc ggcggaggtg
      721 tccgcgccgc gcagcgccgc gtagcggcag agaatcacgg cggtctgctc acgggtgacc
      781 ggctcgtcgg gacggaacgt cccgtcaccg tagccgacga tcagcccgct ctgcgctgcc
      841 cagcggatgg cctgcgccgc gtcggtgtcg gcggggagat cggagaagta cacgtcaccc
      901 tcggcggcgg ggcggtcaaa gagcgtccag agcgtctgcg caagcagcac gcgggtggcg
      961 ggcgctccgc tctcggcatc gcgcagcggc gccggcgtgg cgctgccggg catggtctcg
     1021 gaaacggcag gcggttccgg cgtggggagc gtggcctccg gcgtcacgga aacggcggtc
     1081 tcgacgacgg cgggcgtttc aacaacggcg acggtctccg cggccggcgc cgtggtctcg
     1141 gcgcaggcgc ggaagggcgc ggcggaaagc gcgctgctct cgccggtaag attcaccggc
     1201 acaaagctgc acacggtgct tccctcgctc gaaggggtat aagtgaatgt gatggtgtaa
     1261 gggttatact catcgccggt caggtgctca ctgctgccgt accagacgaa atcggaaacg
     1321 gggaactcct cacccgtcag ctcggagcgg acggcaagcc cagcgtcctc gctgcgcatc
     1381 cctgccgcga cggagagcgc ctcggcatac tgcacacgaa attcgtacgt cacgccctcg
     1441 cgcagcgctt cgccgtccgg cacgcggtgg agcgtcccgg cctcaaagca gtcgcgcttg
     1501 tggacgagcg ggccggtctg atacgttgta tcgccgcgga gaaagtcgct cacggcgagc
     1561 agcgaatggg agagagaccc ggcggagaga gcggaaatat cgttcgtcac gccgatcagg
     1621 ttcgcgctgc cctgtccggc gagaagcgtg ctgtcagcga gcgtcgcagc gccgtttccg
     1681 tcgggcatgg cggaaaggtt ttcccacgcg ccgatggcgg cggtgccgtt ttcagcgtgg
     1741 gcgagcgcca tggcaagtcc gtcggcggca agcgcgcggg cgcttttgcc gttgaagcgg
     1801 atgcggtagt acggattatc gtccttcacg gcataccaca cgccctgatc ctgccagacg
     1861 tagtggagca ccgcctccac gccgccggac gcggcggcgt tggtgtaccc ggcaagatag
     1921 accgagatcg tcgtggtctg cccctgcggc agtgccacgg cgtagctgcg gtaggggagg
     1981 atctccacgt cgtaggaggc gagacggttg gcgtcggtat acggctccgc gcccatctgc
     2041 gcggaaatgt ccttgccgaa ataattgcag ttgagccctt cgtcggcgta gttcagtccg
     2101 acggtgaacg tgaacatctc ggccagacgc ttcgcggcgt tcgtcacgcc gtcgccgtcc
     2161 gtctccgagg cgttctcgcc gaaggaatcc cggtctgcgg cgtaaagcgc cagaacgtcc
     2221 cggaaggtga gcgtttccac ggtaagaccg tccgtgccgg cggcaagcgc gtttccgctc
     2281 agcagcgcaa taagcagcag tgcgatgact gctgcggaaa agacacgata tacggcggat
     2341 cggcgtgagg ctctcatggc agactccctc ggttaaagtc ggaattggtt gcatacttta
     2401 cataacctgt ctgtaaatta acataatgcc cgaagaaaat cgttaagaag aagatacaaa
     2461 ttccgccgcg ttccgttacc gaaatgacgc ttttccagat ttttccgagg aaaatcaaga
     2521 tattgatgcg aaaatcccgg gaagacaccg catatagtgt tacaaaacgg cgcagagatt
     2581 taagaaatat gtcaatctct gcgccgtcgg ctttataaaa ttatgtcgcc caaattacgg
     2641 gaaattattc ctcgccgtca agataacggc acgccgcgag cgcggcgttc gccccgtccg
     2701 aaannnnnnn nnngtggcga gctggcggac gcgcttgccg cggcagtccc cggcaacgaa
     2761 aaggccccgg acgggcgttt cgccggtctc cggtacggcg gcgtagccct gcgccgtgag
     2821 cggcaggagc gcggcgaagc gctcaagctc cggctgctgc ccgacggaga caaaaaaaca
     2881 gtccacgcca agctcggtct cttcgccggt cgcggtattt ttcagccgca gcgcgcggag
     2941 agagtcgtcc tccgtgagaa atccggcgat ctccatcggc gtgaggactg taacgttctt
     3001 tcgcgcgaag aggcgcgcct gcttctcggc gtcgccgcgg aaggcatcgc gccggtggat
     3061 gagatacacg gtccggcaga tatcggaaag aagcagcgcc tcctgcagcg cgctgtcacc
     3121 gccgccgaca acggcggtga cgccgttttt ataaaatccg ccgtcgcaca ccgcacagta
     3181 ggaaacgccg tggccgagaa gctcctcctc gcccggcaga ccgagatggc ggtgctttgc
     3241 gcccgtggcg aggatcacgg cgcggctttc gtatacgccg tcctccgtgc gcacggtgaa
     3301 attctccccg gcacgctcca cggacaaaac ctcgccgacg tcggtctcgg cgtcaagcgc
     3361 gagcatctgc tcgagcatcc gctcggcgag ctccgcgccg ccgacggagg cgacgccggg
     3421 ataattctcc acgcgcggcg cataggcgat ctggccgccg taaccctcgc gttcaagcag
     3481 aagnnnnnnn nnnaatggcg gcggtcattc ccgccgggcc gcctccggca atgatgatgt
     3541 cgtacatggc gattttcccc ctgaaaaaga tcacactgcg ggcggagcag agccccgccc
     3601 ctacgggctg taaaaacctt gtaggggcgg ggctctgctc cgcccaatag tatttgatat
     3661 cgcaaacgaa gcccgccgga gcgggcttcg tttctatttt gtgcggggaa aagcggctta
     3721 ttccgccttc tgcgcggtct cggcgaggag ctttttgatc tcggccacgc cgtagtattt
     3781 ctcaaaatgc acaccgtcgg agagcacgag cgtcggcgcg cccttgatgc cgtattcgcg
     3841 gcagagctcg gggtgttcct cggcggtcag attctcgtag gcaaatccgg ccttatcgag
     3901 catcttctca gccacgcggc agttcgggca ggtcgtgcgg gtgaagagct gcgcggtatg
     3961 cgccgcggaa acggtgccga cctcttcggg ctgcatgccg tgggcgacgg tgccgtcgcc
     4021 gcagcaggcc tcgtggcggt ggatgccgcc ggtgaagtgg ctgtggccga tgttgtactc
     4081 cttgcggtcc ttgaactcct gcaccttgcc gtcgttccag ttctgcacgg gacggtagta
     4141 gccggtgatg cggctccaga cctcggtgtc cttgccgcac ttggggcagg tgaagtgctc
     4201 gccggtaatg tagccgtggt cgtggcagac ggagtacgtc gggctcatcg tgtagtacgg
     4261 cagcttgtag ttctcggcga tcttgcgcac gagatcggcg gcggcgcgcc agtcgggcag
     4321 cttttcgccg aggaaggcgt ggaacaccgt gccggaggtg tacagcgtct ggagatcgtc
     4381 ctgaatatcc agcgcggaga aaatatcctc cgtgtagccc acgggcaggt gggagctgtt
     4441 ggtgtagtac ggggtgccgt tcatgttggc ggtgatgatc tcggggtagc ggttgcggtc
     4501 gtgcttggcg agacggtagg acgtgctctc cgccggggtc gcttccaggt tatagagatc
     4561 gccgtacttt tcctggtaat cgctcagccg ctcgcgcatg tggttgagaa cggccttggt
     4621 gaactcctgc gcctcgggat cggtgagatc cttgtgcagc cagcgggcgt tgaggcacgc
     4681 ctcgttcatg ccgacgagac cgatcgtgga gaagtggttc tcaaaggtgc cgagatagta
     4741 cttcgtgtag ggatagagcc cggcgtcgag gagcttggtg ataacggtgc gcttggtctt
     4801 gagcgagcgg gccgcgatgt ccatcatgtg gtcgagccgc tggtagaact cgtcctcggt
     4861 tttggagaga taggcgatgc gcggcatatt gatcgtcacg acgccgaccg agccggtgct
     4921 ctcgccggag ccgaagaagc cgccggattt cttgcgcagc tcgcgcagat ccagcctcag
     4981 acggcagcac atcgagcgca cgtcgctcgg ctccatgtcg gagttgatgt agttggagaa
     5041 gtacggcgtg ccgtacttgg cggtcatctc aaagagaagg cggttgttct cggtctcgct
     5101 ccagtcaaaa tccttggtga tggagtaggt cgggatcggg tactggaagc cgcggccgtt
     5161 ggcgtcgccc
//
LOCUS       DAWAAE010000143         5062 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6752_length_5062_cov_3.202716, whole genome shotgun sequence.
ACCESSION   DAWAAE010000143 DAWAAE010000000
VERSION     DAWAAE010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6752_length_5062_cov_3.202716"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1904..1913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1026 a         1620 c         1481 g          925 t
ORIGIN      
        1 tgctcgcgct caacaagagc gcggagagcg tggagatcac gctgccggag agcgccttta
       61 cgcagaagac cgtggacgtg acggacgacg acggtaacgt caccggaacg aaaaccgtcc
      121 gcgtggcgga catgaacacc gtctccggca ttttgaagga ctacggcctc atcaactaca
      181 agtggctctt ccagctctat tcgcgcttct cgaacgcgga ggtgcagctc agccccggga
      241 cgtacgcgct cacaacgaat ctggactacc gcgctcttgt gaaaaagatg cagaccggcg
      301 cggattcgca gctccagacg ctcgtcatgt tccccgaggg ctacaacctt gaccaggtgt
      361 tcgccaggct cgaggaaaac ggcgtgtgca gcagggagga tctctacgct gccgcggcga
      421 acgccgagtt ctcgtatacg ttccttgaag gcgtggagac gggcgatccc taccggctcg
      481 agggcttcct cttcccggat acgtattact tctatcaggg gatgcaggcc tcgagcgcga
      541 tcaacaaatt cctctcaaac ctccattacc gcatcacgga cgagatgtgg cagaaagcgc
      601 aggccatgaa tctgacgttc aagcaggtga tcacggtcgc ctcgctcatc gagcgagagg
      661 ccgcgaacga cgccgagcgc gcgacgatcg cctcggtcat ctataaccgc ctgaacatcg
      721 gtatgccgct gcagctcgac tccacggtcg tgtacgccat ccgcgagacc ggcgagacga
      781 cgatgtccac cgagctcatc caggccaccg acagcccgta taacacctat ctctatcagg
      841 gcctgccgcc cggaccgatc tgcaaccccg gcatgaagtc tatcgaagcc gcgctcaacc
      901 ccgagcagac cgggtattac tactttgcgc tcgacatgga aacgaagagc atgcgcttct
      961 tcgccacgct cgccgagcac gaggcgtttg ttgccacaca ggattacggc taaacgcttc
     1021 aaacaaacct tgtttgactc ctgcggaggc aaacccgggc aagacatttt aagcacaaaa
     1081 gaccgtggaa aacattctgc ggtctttttg cattgtaaac gcaaaaccgg cgtgatataa
     1141 tcgcaactat ggaaacgaac gatcttattc ggcgcgcgga ggatcttgcc gcgcgctgtg
     1201 agcgcacctc gtcggtgacg gcgaccgcct ttctcactcc ggcggagcgc atggagctgc
     1261 aaaatgcccc ctcgctgcgc tacgctgccg tcgtctattc cggcggcggg acggagtgcg
     1321 agcgcacctg cgcgttcttc ctcccgttct atctcacggc ggaggagctt gacccttcgg
     1381 agtatatcag cgcggtgcat ttccgcagct tcttcggcga gccgggccac cgggattatc
     1441 tcggcgcgct gctcgcgctc ggcgtgcgcc gcgagtgggt gggggatatc cgcatccggg
     1501 gcgcggaggc gtgggtgttc tgcctgccga gcgtcgtaaa aacgctcgcg gagctcgacc
     1561 gcgccgggcg ctataccgtc cgcggcgagg agatcgctct cgcggacgtc ccggaggaag
     1621 aggtgaagac cgagaccgtg agcttcacgg tgcagagcct ccggctcgac gccgtgaccg
     1681 cggggctttt ccatgtctcc cgcacgaccg ccgcagagct catccgtctc ggcagcgtgt
     1741 cgctcaacta tgtgccgtgc ctgcacacgg acgccgccat ccgcgacggc gacgttattt
     1801 ccgtgcgcgg caagggcaag ggaacggtca ccgacaccgg cggccgctcg cgtaaagacc
     1861 gtatcttcgt caccgccgaa cggcgggtct gaaaatataa aggnnnnnnn nnnaccatga
     1921 tccctgaaag actctcccgg ctgcgccggg aaatggaaca gcgccgcatc gacgtgtacg
     1981 tcgtccccac ggcggacttc cacgaatccg aatacgtcgg cgcgcatttc aaggcacgct
     2041 cctacatcac cggctttacc ggcagcgccg gtacggccat tataacaatg accgaggccg
     2101 gtctctggac ggacgcgcgc tatttcctgc aggcgaagca ccagctggag aataccggcg
     2161 tgacgctcta ccgcatgggc gaggagggcg taccgacgat catggaatac ctgcgctccg
     2221 cgctgccgga gggcggtacg ctcggctttg acggccgcgt catcaacgcg cgcctcggcg
     2281 cggctttcct tgcgcttgcc ggggaaaaga acgccaagct tgccgtcggt gaggatcttg
     2341 tcggcatcat ctgggacgac cgccccgagc tgccgcacgc gccggtgcgc atcctcacgg
     2401 cggaatactc cggccgttcc accgcggaca agctcgcgga cgtgcgcgct gagatgaaaa
     2461 agcagggcgc gagcgtccat ctgctcacgt cgctttatga tatcgcgtgg ctgctgaacg
     2521 tccgcggcgg cgatatcgac tatgtccccg ttgtgctctc ctatcttgcg ctcacggagg
     2581 agcaatgctt ctggttcgtg caggacgccg ttctcacgga cgagcttcgc gcctatcttg
     2641 ccgagaaccg catcgaaacg cgcccgtatg agagcttcta tgactatgcc gccgccatcc
     2701 ccgccggaga gacggtgctg ctcgacaagg gcgtgtgcaa ctaccgcctc gtctccgagc
     2761 tttgtgatgg cgtcaagatc gtggacgaga cgaacccctc cgtgcttatg aagtccatca
     2821 agaatgagac cgagcagaag aacacggtca acgcccacat caaggacggc gttgccgtga
     2881 cgaaattcat ctactggctc aagaagaaca tcggcaagac gccgatgacc gagctttccg
     2941 cctccgacta tctcgccgcg cgccgcgccg agcagaaggg ctttctcgat ctcagctttg
     3001 acaccatctg cgcctacggc gcgcacgccg ccatcatcca ctacgccccc acgccggaga
     3061 cggatatccc gctcgaggcg cacggcctgc tgctcgtgga ctccggcgga cactatctcg
     3121 agggtacgac cgacatcacc cgcaccatcg ccctcggccc cgtgacggac gccgagcgcg
     3181 ccgacttcac gcgtgtcctc cgctcgcatc tgcatctggc gaacgcccgc ttcctccacg
     3241 gctgcacggg gctgaatctg gatattctcg cccgcgagcc gctgtgggag gctgatctgg
     3301 actacaagca cggcaccggc cacggcgtcg gccacatcct gaacgtccac gaagggccga
     3361 acggcttccg ctggcggcag tcgccggagc gcagcgaggg aagcgtgctc gaagagggca
     3421 tgatcacgac cgacgagccg ggcctttaca tcgaggacgc ctacggtatc cgcatcgaga
     3481 gcgagcttct ctgccgaaag ggtgtcaaaa acgagttcgg gcagttcatg tacttccaaa
     3541 atctcacctg cgcgccgatc gacctcgacg cggtcgacgt taacgccctc accgtgaccg
     3601 agcgccgctg gctcaacgag taccacgccg ccgtgtacga gaccgtcgcg ccgcacctca
     3661 ccgagaatga gcgcgtctgg ctgcgcgacg tcacccgcgc catctgattt tgcacgttat
     3721 ctgcttcggg aaggagaaca cccatgcttg atgaagccgt tcagaaagag atcattcaaa
     3781 agggacgcga ctttctcaaa agcccgctgg agcgggatcc ctacgaggaa gcgttcgagt
     3841 ccgatcagga ccggaagctg ccgcagccgc cgctcgtgaa agcgcccatg gcggaggagg
     3901 gcgagcgcat cgctctcacg agagattttt ccggccttgc gctgaaaaac gatcttgtga
     3961 gcctcatccg cgaccggcgg agcgcccgga tctatacgaa tgaaaccatg acgccggacg
     4021 agctctcgtt ccttttgtgg gcgacgcagg gcatcaagaa gatccgcggc aaggcatatg
     4081 cgaccatccg caccgtaccg tgcggcggtg cgcgccacga gtttgaaacg tatctgatcg
     4141 tccggaacgt ggtgggtctg cggcccggcg cgtaccacta tctgccaatg ctccatgcgc
     4201 tcgagttcct gcacccgatc gaggacattc cggaaactat aacccagacg ctgtgcggcc
     4261 agaaatgggc ggccaaggcc aacgcggtgt tttactggtc ggtcgtgccc taccgcgccg
     4321 agtggcgcta cggcatttac gcccaccgca tcgcgctcat cgacgccgga cacgtggggc
     4381 aggcgctcta tacggcctgc accgggctgg gtctcgggac atgcggtctc ggcgctttcc
     4441 agcacgagaa atgctgcaat ctcttcgggc ttgacggcga ggaggagtat atcatctaca
     4501 ctgcccccgt cggcacgatc cgcgacgagg acctgaccga agaaaacgcc ttctaccagt
     4561 tcgtggaaga cgagggactc tgaattttat agcgcaaaaa agctcacgga tccgatccgt
     4621 gagctttttg cgtgtatgcg gtctctatct cccgcggagc ggctcagtac cgatccagcc
     4681 gccgctggat ctcgtcgata tcgctctggt caaagtcttc cgcgcgcaga ccttcctgcc
     4741 ggcacagctc ttcaaattcc tcgtttccga tatggcaggt gccgcggcgc acgatggcat
     4801 cgacgatctt gcggatacgc tcctcgcgcg cgtcgtcgtc cagtatctgt gtcatcatcg
     4861 ctacttccag aacatcgtcc caggagtcga ttttcccgtc gccgttccag tctatgctgg
     4921 tatgtgaatc cagaaaatcc agaagaaaac ccataaacgc gcctccttac acggcttacc
     4981 atgataaaaa agacacgata ccgcgtcggc ggaaagaaga gaaagcattc cgacaggggg
     5041 gatcgtgtct ataatcatta ta
//
LOCUS       DAWAAE010000144         5047 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6776_length_5047_cov_2.518830, whole genome shotgun sequence.
ACCESSION   DAWAAE010000144 DAWAAE010000000
VERSION     DAWAAE010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_5047_cov_2.518830"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    623..632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1777..1786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a         1479 c         1220 g         1309 t
ORIGIN      
        1 acattgaatg ttccagctcc ccattcctcg agcatatacc cctggagctt tccgattcca
       61 agattcctgt actggtggtt atttatattt tcttcaagct gtttgattgc tgcctcgatt
      121 gcacttacat attcctgtcc catatgggca gcaatatccg aacccattat tccggtagca
      181 aattcccagc cttgctgcat ctcggttttt gactgcatag gccgttacct cacatattca
      241 taaacttttc aagcccgtca ttgtagagac tgactactcc atagaaaagc atctgccgtt
      301 tcatcaacaa cttgtttctg aaaacctcct cagactttga agcgacctgt gtgccaattt
      361 ttccaatggc atctgcaccc gttgagaacg tcttcttgat gcatttcagg atatcggcct
      421 ttttatgctt tttctcatca gccataaccg tttcggtatc tgcattttcg agaacatcct
      481 ctgcaggaag ttcgactgcc aactcagcat tctggtctgt acccgtggcg gcgatgcttt
      541 cctttgtgac cttgaggaaa ctcggaacag gaagcgcaga gagcttttcc agaaacgcat
      601 cataatcctt gcgggtggtc agnnnnnnnn nnatatacga cagcctgatt acctgcccag
      661 ccgaacttga ctccgcattc atcaaacttc acatcaataa ctgggatcaa tttatctgtg
      721 ccttttatct ctccgagaaa gagaattttt ccttggatgt ttccggcttt cttgttaccc
      781 agccagacct tttccgtcca agagacgatg ttgatagaat catccctggt gccgatggca
      841 ccatgctcat tgtcatcgtg tgtttccacc atcttcttta gctggttcat caacaactca
      901 tccttatata caacgatgag agttttcttt gcttcaaaca tatgcattct cctttgtgta
      961 gcccccggtt tcgcgggaac gatattcata ttgcttgcct atctaaaaag tgttcttcta
     1021 taattgcagt cagttgagcc gcaatttcca aaaatctcgg ttcaaactga gcgcttttgc
     1081 actgatttaa ctgctgctca cccgatagat actgcttggc tgtattgccg cacctgttgc
     1141 gctatatagg tgcataccgg acaatcaacc gaaccagttc cagaaaccgc catccctatc
     1201 tttatcctca ccagaagaat ttgtagtaga acctgtagct ttttctcggc ttcctctatc
     1261 tgcttgataa tttccttacg tgtagcattc catttatcct cgccctcaga tgcccagcta
     1321 tcatcccaat acagagaata ggcctttaca taagcattta caaaatcctc atttttactg
     1381 tctatcataa tctgatcaac tgtagcttgt agttcctgtt tcttggctcc cgcatttcct
     1441 acggcggata taataaggca catcgcaata attgcgccga aaacgccaaa ggcaatagca
     1501 aaagtgcgcc gctcgtcttt ctgcttgaat tccatacggt aacgctcttg ggcatcacgt
     1561 ttctcttgga gtcactcctt atgttccatg tgctttaccc tcactttggc atcgtatgcc
     1621 gccttgctct ggccttggag cataatccta tatccgcact atttacaaaa gaatgtatca
     1681 agtccatcct cgatccctaa aaacttgcac cgcagttagg gcaaaataat gatttcagtt
     1741 ccatactatt ctcccatatg taattgtcag tcgtttnnnn nnnnnnctgc tcaaaagcct
     1801 ccttgtcccc ggatttgacg cgattcagga tttgcgcata ctctgttgcg ccgattttat
     1861 cctcatcctt atatcgccac cacatgtcag gaaactcgtc cgtgttggtc ataagctcca
     1921 gcactgatgg atcgaaaacc tttgaaaaac gaatattctc aacccacgcc agcggaacag
     1981 tctcagtagc atatctgctt ttatgcctgt tcagcccgat caccgcgacg ccgttgtccc
     2041 acaatctcct gctgatatat accttcctgc cacccggaaa cgaagccgtt ccatatcgaa
     2101 gcactccatt ttcatccagg tgctccatca caatgttgcc aacaacgcaa tacttcatca
     2161 tccccggctc aattccatta ctcatctaat taccttcctt cctgtagtat tcgttgattt
     2221 ataatcatcc aacagcgcag atggcccctg cctcttacgc cttgataagc catactctgt
     2281 ttttttcatc gggactccac aggctctgca aatctttttc tatttccatt ctccgatccg
     2341 ttgattggcg agaaagcgtt cgagatcgtc cggcagcgga gcggtgagct ccagcgtttc
     2401 cccggtgacg gggtggcgga gcgaaagata cgccgagtgg agcgccggtc gggagagcat
     2461 atcgtccggc gagccgtaga gagcgtcgcc aaagagcggg tggccgatgg ccgccatgtg
     2521 tacgcggatc tggtgtgtgc gccccgtgag cgggcggacg cgcagcagca cgcccgtggg
     2581 aaagcgctcc agaaccgtat actccgtgcg cgacggctgc ccgtccggtg tgacggcgcg
     2641 cttcgttggc gcggcttcgt cccgcccgat gggaagatcg accgtgccgg aatcgtccga
     2701 aaatgcgccg cgcgcgagag caagatattc ccgccggaag gcgtccgtat gcagcatccg
     2761 gcgcagaagg tcatggacat acgcgctttt tgcgatgacc atgatacccg aggtaccacg
     2821 gtcgagccgg ctcacgggat gaaacgccgc gtctctgccc cagagcgcgg caagggcgtt
     2881 tgcaaccgta cacccctgcc ccccggtcga gccgtgcacc gccatatccg ggcttttatt
     2941 gagcacggcg atgtcctcgt cctcatagac gaaggtaagc ggtgcggcga tgggcagcgc
     3001 ctcgttcacc gcgccctcgt cgccgatctc gacgcaaagc acgtcgccgg tgcggagctt
     3061 cgcattggtg aaaacgcgct cgccgtttcg cacgagtccg gtttcgcgca gcttgacccg
     3121 ggcaatcaat ccctcggcca tatgcagccg ggcgcggagc aggtgcttta ccgtgctgcc
     3181 gttccacgcc tcgtccatgg tgagcgtcaa tgtgcgcata cgccgatttt cctccgttcc
     3241 gccgtcaagt ttccgttccc ttttcgttcg ggatatggta tgatgaaaat atcccaccgc
     3301 ttctctgccg aatgctctga cgggaaggat gttttttatg atctatcttc gatgcgtatt
     3361 catcatagca tatttgacga tcctttgtct ctacttcttt tcggagacaa gcggaaattt
     3421 caagcgccgc gccgtaaata agatcgcgct cgccgtgctc ttcgaggcct acgccgtgag
     3481 cgcgtatctg ctgcactcgc cgctgttcag catgcggatg atattgccga tcggactctt
     3541 ttttgcgttc ctcggcgaca ttttcctttt gtggaatttc gtccgcggcg gcatctgctt
     3601 cagcatcggc aatttctgtc tgctgttcta tgagatcatg ctcatgcggt atgagcgtct
     3661 tccgctgcgc atcctgtttc cggccgctgc ggtctttctc ctgttctggg gaacgacgat
     3721 gctcttatat caaaaggagt tcatcgattt cagcagcgtg cgcatttttc cggcgtatct
     3781 tttcagcgtt tcaatgcacg gctctctcgg cgcggtgctc gccgccgtgt gtccgagtcc
     3841 cggcgtgaag ctcttcggcg cgggcgtttt tctgatgatg atctccgatt acattctttc
     3901 cgtacacaaa ttcaagcacg gcgcaaactg gatcctgcgc tgcaacagcg cgagctactt
     3961 tgtcggcacg ctgctcgcgg cgctgagttt gtcgctgccg atctgaggag gcggttatat
     4021 ggccgagcgc ggccgcttcg ccccgacgcc gagcgggcgg atgcatctcg gcaatgtgtt
     4081 ctccgcgctg ctcgcgtggc tttcggtccg cgccgcgcac ggcgagctcg tgctgcgcat
     4141 cgaggatctg gatacgctcc ggtgcagcgc ggaaaacgcc gaaattttaa aagatgatct
     4201 gcgctggctc ggtctcacgt gggacgctga gcaaccggat cagagcacgc gcagcgaata
     4261 ctatgagagc attctcgacc ggctgcgcgg tgagggcttt gtctatccct gctggtgtac
     4321 gcgcggcgat ctcaaaaacg ccgcgaacgc cccgcacgcc tccgacggtc acccgatcta
     4381 ccccggcacc tgccgcgatc tgaccgaaga ggaacgcgcg gcgaaagcga aagtgcgcgc
     4441 cccgctctgg cggctgcgtg tgccggacga gaacattgtc ttttcggacg ggcattacgg
     4501 cccatacgcc gaaaatctct cgcgcgagtg cggggacttc attctccggc gcgcggacgg
     4561 cgttttcgcc tatcagctcg ccgtcgtggc ggatgatatc gacgccggta taacgcaggt
     4621 cgtgcgcggg cgcgatcttc tctcttccac gccgcggcag ctgtatctct accgtctgct
     4681 caacgccgca ccgccggaat actatcatgt ccccctgctc accgcgccgg acggacggcg
     4741 gctctccaag cgcgaccgtg atcttgacct tggtattctg cgcaaagcat ataccgcgcc
     4801 ggagcttctc ggcgcgctcg cccacgccgc gggactgctg gaccaacggg aaccgatctc
     4861 cgccaaagag ctcatcccgc tcttcgactg ggcaaagatc cccaaagacg atattgccag
     4921 cgatgtataa aaccctcgcg ccggaacatt cggcgcgagg gttttagcgt gtcaaaaagg
     4981 gatttttgac atgctctcaa acgagaccca ctgcgttggg ctctcgtttg aaaaggctgc
     5041 ggctcgc
//
LOCUS       DAWAAE010000145         5045 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6779_length_5045_cov_3.987174, whole genome shotgun sequence.
ACCESSION   DAWAAE010000145 DAWAAE010000000
VERSION     DAWAAE010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6779_length_5045_cov_3.987174"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1060 a         1515 c         1437 g         1033 t
ORIGIN      
        1 actcctacgg cggcatgtcc ggccgtgaca tcatggctct ggcctgcggc ctctatgaga
       61 gctgcgactt cggctacatg catgaccgcg tgcagcagtg cgagtacctg gcacagggct
      121 tctacaaggc aggcgtcaag ggcgttgttc ttcccgccgg cggccacgcc gtgtacatca
      181 acatggacga gttcttcgac ggcaagcgcg gccacgagac cttcgcgggc gagggcttct
      241 cgctcgagct gatccgccgc tacggcatcc gcgtctccga gctcggcgac tactccatgg
      301 aatacgacct caagaccccc gagcagcagg cggagttggc gaacgtcgtc cgttttgcca
      361 tcgaccgcag ccgtctcacc caggagcatc tggactacgt catcgccgct gtcaaagcgc
      421 tgtatgaaga ccgcgaatcc atacccaaca tgcgcatcat ttggggccac aatctcccga
      481 tgcgccactt ccacgcgttc cttgagccct accccaacga agagaagtaa gaagtaagaa
      541 gaagtaaagc aaaaccccgg agaaactgag aaagcgcata ccagcgaacc cggacggcac
      601 ggcgccggtc cccaccggcg tcgtgcgtca gtcgaatcca tcaaattatt ggagggaaaa
      661 tcaatggcag acaaacagct tacctcaaac cgtgacggct ttaccagccg gtggggtttc
      721 atcctcgcct gcatcggttc cgccgtcggc atgggcaaca tctggcgctt ccccatcatg
      781 gtctcggtat ggggcggaat gacgtttctt atcccgtact tcatctgcgt tctcctgatt
      841 ggttctaccg gcgttatcgg cgagtttgct ctcggccgtg cgaccggcgc cggccccatc
      901 ggcgctttcg gcgaatgcac ggctctccgc ggcaaccgca agatcggcga aggcatcggc
      961 cttatcccgg tcatcggctc tatggctctc gcgatcggtt acacggtcgt catgggctgg
     1021 atcttcaagt acacctttat ctccatcacc ggcgatatgc atgcaatggg acaggacatg
     1081 gcgtccatcg gcggccactt tgacgttgtc gcggtgaata acgtcccctg catcattgtt
     1141 gcgatcctcg cttcctttgc catcatggcg ctgggcgtat ccggcggcat tgaaaaagcc
     1201 aataagatca tgatgccggc gctgtttgtc ctgtttgtgg ttctcggcgt ttatatcgcg
     1261 tttcttcccg gctccggcgc cggctacaag tacatcttca cgctcgaccc caaagggctg
     1321 ctcaacccgc tggtgtggat tttcgccttc ggccaggcgt tcttctctct gtccgtcgcc
     1381 ggtaacggtt cggtcatcta cggctcctat ctgccgaaga atgagaacat cgtgacctcc
     1441 gcgagaaacg ttgccgtgtt cgatacgctc gcggctctcc ttgcggcctt cgtcatcatc
     1501 cctgcgatgg cggcgggcgg cgcgcctctg gagaaaggcg gcccggggct catgttcgtg
     1561 tggctcgtca acgttcttaa cggtatgccc ggcggccgca tcatcggcgt tatcttcttc
     1621 atctgcgtcc tgtttgccgg cttgagctcc attatcaacc tgtatgaggc tccggtcgca
     1681 acgctgcagg acaacctgaa gctcaagcgc gtccctgcaa cggcgatcat tctcgctgtg
     1741 ggcggcattg ttgccatttt tatccagaag atcacctccg agtggatgga tgtcgtctcc
     1801 atctacattt gtccgcttgg cgcgctgttg gccggcgtga tgttcttctg ggttgcggga
     1861 aaggactttg cgctcaagga ggtcaaccat ggcctcaaat ccccgatcgg cggctggttc
     1921 tatccgcttg ccaagtatgt gtactgcatc ctctgcgtta tcgcgctggt tgcaggcgcg
     1981 gcgctcggcg gcatcggctg atcgacccga tccaataaaa acgcgcgtat cggttctccg
     2041 gtacgcgcgt tttttcttgc tctatggcaa aaaccctgct acaatgaacc accggatggc
     2101 gaaaaccgtc taacgaatat cataaaatgc ttttaggaac gaaacaccat gaaacaaagc
     2161 gacaatacaa accgaacgcc tgccaattgg gacggcacac cgcaggagga ctattttgcc
     2221 gactggaaca ctccgccgga ggaatatccc gccgcgggac ggcatctctc gccggaagcg
     2281 cgggaagaat actatgccga gcgcgacgcc tactacgccc gccgggacgc ctactatgcg
     2341 cagcgcgatg cctactacgc ccagcgcgag cgcgaggagc gcgccgcgga cgaggaaggc
     2401 gaagaatacg aggagaagta cggcgaagac tgtgccgacg acgcggacga gccggaggag
     2461 gaagaggacg acgtttccta tgcccccgag gaccgctccg aaacgctgga ggagctgaaa
     2521 gagaagccga aaaagaaaaa gcgccgccgc aaggggggct ttctgcggtt tcttgtcacg
     2581 gtcatcgtgc ttgcgatcgt cgcgctggtg gtcatcggca aagcgccggt gcgaaatccg
     2641 gataaccttg agcgtatttc gggccgcagc acggtgcttc tctgcggcac ggacgaggac
     2701 ggcatgcgaa ccgatacgat ccttctcctg acgctcgacc ggaacgaggg cagcatccgc
     2761 cttttgagca tcccgcgcga cacctatgcg cctgcctact atgtgccgaa gatcaactcg
     2821 gcctacggcg cggtcggcgg cggggaaaag ggcatggagc agctcatgga gcaggtgaaa
     2881 aacgtcgtcg gcttcatgcc ggacggctat gcgctcgtta atctcagcgc ctttgtggag
     2941 gcggtcgatc ttctcggcgg gctggatttt gacgtgccca tggacatgga ctacgacgat
     3001 cccgaccaga acctttttat ccatctgaaa gccggcgagc agcacctgac cggcgagcag
     3061 ctcatgtggg tcgtgcgttt ccgctcggga tacgccaatg ccgacatcgg ccgcacggag
     3121 gtgcagcgcg cggtgatgaa gaccgccatg aagcagtggc tgcggccgaa aacgctcgct
     3181 gcgctgccgg ggctctggcg catatatcag gaaaatctca cgaccgacct ctcggtgcgc
     3241 aacatcgtct ggatggcgcg cgtgctcctg aagggcatga gcggcgatat tgaagccaac
     3301 gttcttcccg gctacgccac catggcgggc gatgcctcgg tctacatgat cgacacctac
     3361 gccgcctcgg ctatattgcc ggattacagt ccctataaat aaaggaaagc gccccggctg
     3421 cggccggggc gttctgcttg gttcgcgtgg tgagaaaaac ccttcagtca gcctgacggc
     3481 tgccagctcc cctgttaggg gagccaagat agtattccga taacctgctt gcctccccta
     3541 acaggggagg ggttttttgc gcggggagac gaacctcctg tggaaagtaa atgctgaaaa
     3601 ccgcgacaaa gaaaatataa tttgaaaaat aatttatata aactattgac aaaagttttt
     3661 cttgtgctat actatggtca aaaggtcaaa ataggtcaaa ggagatggtt ccttttgaaa
     3721 aagacattgt acagtctgat gctcaacgag gaggtcgtcc gcgaggtgga cgcgctcgcg
     3781 caccggctgg ggacgagccg gtcgaatctc atcaatcaga tcctcgccga atatgtgaac
     3841 tatacaacgc ccgagcgccg catcaacgac attttctccg cgctggagca gctcatcagc
     3901 ccctcgcgtg agcttgtgcc gtttttctcg ccgaacagca gcacgatgtc gctcaagagc
     3961 tccttgcagt ataaataccg cccgacggtc aagtacgagc tggagctgca cggcggcgac
     4021 gaaaactcca tcggcgagct gacggtgctt ttccgcacgc agtccgaagc gctcatccgc
     4081 gcgatgacgg agtttttccg gctttggaag cgcattgaag atgcgcacct gcccgccgga
     4141 acggaacggc agtatgcact gtacgccggc aagttcgtgc gctccatcgc gccgcccacc
     4201 aaggactgta ccgccgacga gctggcgcag gccatcagcg aatacattca gctttttgac
     4261 cggctgatga agggctatgt gagcggcgag ctggacgcct gtgctatcga gcgcgagtgg
     4321 ttcacgcaga tgcgccgcgc cgctgtgctg atttaatcct gtataccatt tcttctctta
     4381 atttattaag gagggatctt tatgaacgct gaaaaattca cccagaaaag tctggacgcg
     4441 gtccgcaccg ccaaatccat ggcggaggaa aaccgcaaca actatatcac ccctgagcat
     4501 ttgctctatg ctctcgtcga tcaggacggc gggctgatcc cgtccctgct cggcaaaatg
     4561 aaggtggact gcaacgccgt cctctccgag ctcgataccg ccatcgccgc gctgccgaag
     4621 gtcggcaatg tgagcgaggt gtatctctcc ccggagtgcg accgcgccat caatgccgcc
     4681 gagaaagccg ccaaatcgat gggcgacgag tacatctccg tcgagcatct gatgatcggc
     4741 atcttcgccg ccgccacgcc ggcgatcaag cgtattttct ccgaccacgg catcacgaag
     4801 accgccttta ccgaggcgct ttccaaggtc aagaccggtc ccgtgaccgg cgataacccg
     4861 gagagcacct atgacgctct cgccaaatac ggcaccgatc tcgtcaagcg cgcccgcgaa
     4921 aacgagctcg accccgtgat cggccgtgat aacgagatcc ggaatgtcat ccgcattctc
     4981 tcgcgtaaaa cgaagaacaa ccctgtgctc attggtgagc cgggtgtcgg caaaaccgcc
     5041 atcgc
//
LOCUS       DAWAAE010000146         4957 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6930_length_4957_cov_2.714810, whole genome shotgun sequence.
ACCESSION   DAWAAE010000146 DAWAAE010000000
VERSION     DAWAAE010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_4957_cov_2.714810"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    929..938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2273..2304
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3829..3838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1009 a         1424 c         1424 g         1048 t
ORIGIN      
        1 gtacacttcc gcattacgac cgagccgaga aagggcacgc tgacgcttga agaaaacggc
       61 gaatttgtct acacccccgc cgaaggcaag cgcgggcgag attacttcgg ctactgcgcc
      121 tcggatccga aaggcaatgt ttcgcaggaa gctaccgtcg tcatcacgat ccgcaaggcc
      181 gggaaaggcg ccggctatgc cgataccgcc ggtctctcct gcgagtacgc cgcgtgcgtc
      241 ctcgcggaag aaggactctt caccggccgg tgcgtgtgcg gacaatatct ctttgagccg
      301 gaggagacgg tctcctgcgg cgagtttctg tcgctctgca tggaaacggc aggcatcgag
      361 ccggtggaaa cgactgtttc cgcagaaagc agcgccaccg ccgcccccgt gtggatacag
      421 cagtatgtcg atgccgccgt gtccgagggc tgccccgtgc cgacgtttga cggagcctcc
      481 ttcgacgcgg acgcgccggt aacgctcggc gccgcggcgc agacactctc caaagcgctg
      541 cgcatcacgc cgctctcgtc cgcgagcggc gaagagagcg ccgcagcgcg cgccatacag
      601 gagctggtgg cgtgcggtct tctgccggtc ggaatggatg cggaagcgtc tctcacgcgc
      661 ggtcaggcgg cggagctttt cctaaaggcg ctccatcttt gggaacagcg cagctgacaa
      721 aaaaccggca gcactcaact tcggttgggt gctgccggtt ttgggtgttc aagggtggaa
      781 aatcgctttt tttcttcgca aagatcgcct ttgccgtgca aactgtctat tcatgactgc
      841 tcaaatggct ttaaccagaa aaaaattgct ttttgatgca gcataatccg gagaacctct
      901 tgacttgaaa agcaggtaaa gtatatggnn nnnnnnnngt atatggttga tgtgttataa
      961 ttgttaaaac aattaacgat agcaggtaca tataatgaaa aaagacagca gaaaatacta
     1021 cgccgcctat gaggaacgct acaaaaccgc ccatgcaaac ggtgttagtt ggtcaagcga
     1081 tgtaaatacg cctatcgtga tggaagtgat tgacaggtat aaaatccgtc gggagcataa
     1141 actccttgag atcggatgcg gggaaggccg agattccagg acggtcttgg atcatggttt
     1201 tccgttaatg gcaacggaca tttccgaaga agccattgcg tattgcaggc agcagatgcc
     1261 ccggtacgaa aaacatttca gcgttttgga ctgcttatcc gcccgactgg aagaaacgtt
     1321 tgattttatc tttgccatag ccgttgttca tatgctggtg cttgacgagg acagagacgg
     1381 cttttatcgg tttatccgca gccacctgac cgcagaggga aaggctctga tctgcaccat
     1441 gggcgacgga gaatttgaaa tgcagagcga catttccact gcatttacct tacaggagag
     1501 aaaccacgac tccggcaaaa taatggttgc cggaacatcg tgcaggatgg tatcatggaa
     1561 tacattggaa aatgagttag cgagaaacgg cctgaccatc attgagaaag ggatcacaag
     1621 ctcccttcct gattttaact gcctgatgta tgctgtcgtt aaagcataag ggagcgtata
     1681 tacgaggatt tgacagtgaa taaagaagaa ttattgaagc tctctcccgg ggagttgacc
     1741 aatttgggca tctgtccgac ctgtttcaac cgggaaaacg gcggtgcgct ttatggtgac
     1801 aactcggata agatgctcta tgaggatgaa gatattgaat gcttctttgt cggcaatccg
     1861 agagcggccg gtcatatgtg catttccact atggaacact atcacgacat gagcgaagct
     1921 cccgatcaca tcaatgagaa gatcattcga tttgcgaagc ggttcatgca gatcctttgc
     1981 gaggtttatg ggtgtgagcg ggtctacctg tgtaccatgt gcgacggtct caacaatcat
     2041 tatcacatcc agcttatccc ccgctaccag gacgaaaagc ggggcagcca aaactttgta
     2101 aagccccgca aagcgtatgt cccggatcac gccaaattgg aaaaagtaag aacacgaatt
     2161 cacgaatata cgcaagaatg attttcagaa acatacttgc tgaaacgctt accggcaggg
     2221 tctcccctgc cggttgagct tgtcaaaaac acttttttga caagctctca aannnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnntctgta tttcagtctt ttcgcagccg ttcggccagc
     2341 gcttcgtcgg cagcggtgat gatcgtacgg taatccgtgt agatcacccg ccccggcttt
     2401 gcgccggagg gctttttcac aaagcgcacc tgcgtgcagt ctacagctac cttaccgccg
     2461 gttttcgcct gcgaatacgt tgcagcgagc gccgcagcct gccggatcgt ttcctcgtcc
     2521 gtctcgcccg ccgcctgcga cacgatcaca tggctgccgg ggagcttctg cacatgcagc
     2581 cagagatcgg tgcggcgggc gatatggaac gtgagctcat cgttctgcac gttgccgcga
     2641 ccgacaagga tctccgtgcc gctgtcggat acgaaccgca gcggctttcg cgctgcgggc
     2701 tgcttctgcc gcttgccctg cgccttctct ttgatatatc cggcgttcac cagctcgcgg
     2761 cggatgtcgg cgagatcgcg gtcgcactca gctcgggaga gctcgtcgag cacgctctcc
     2821 agatacgcct cgtcctcgcg cgctgcggcg atcagctccg tgagcatccg gtttgcggtt
     2881 ttcgctttgt tgtagagctt ataattgtaa gcggcgttct gctgcggcgt tttgcgctcg
     2941 tccagcggca cggtgaccgt cgggcagtcc ggctcataat aatcctgcgc ctcaaagaag
     3001 cgcatgccgc gcttcaggcg gtacatattc gccgtgatga gatcaccccg gcggcggtat
     3061 tcctcgcgtt tttccgtttc gagcagttcc tgctcgcgcg ccgcgatctt gcgcaggagg
     3121 cggtcacgcg ccgttttcgc cgtgcgcgtg agatcggcgc tgcggcggcg cagattttcc
     3181 tgctgatcct ttttgcggta aaacgtatcc agaagctcgc tgaacgacga aaactccgtc
     3241 agctcggcgc ggttgccgta ctgatacacc ggctggaacg tataatcctt cggcatgccg
     3301 tcaatggaga tcatcaccgg tgtgaagtcc ccggtatcga tgcgcgcgcg cagcgcgtcg
     3361 agcgcggcgt cgagcccttc ccagccgccg agcgagagct cgcggcagag aagcggcgag
     3421 agtccgccga acgtatcgag cagccactta tccggcggct gcgtccggtc ggcgtgtgtg
     3481 agcagccgct cgcattcttc cctctccgtc cggaaaaacg acggtttctc ctgctgcggc
     3541 ggcagcgtgt agaaaagtcc cggcagcagc gcgcggcgat tcgggtcctc atagtccaca
     3601 cggcggatgc agtccaatat atgcccgtcg ccgtccagca gaaggagatt cagccccttg
     3661 cccatgagtt caaggacaag cgttttctcc ccgcggcagt tcagctcgtc cacggtatcc
     3721 aaccgcagga taagcagccg ctcgccgagc ggctgttcca gcgccgtgat gcgcgctccg
     3781 atcagatatt tgcgcagcag catgcagaac atcggcggct gcggcgggnn nnnnnnnngg
     3841 tctgctccgt gagagaaatg cgggcgctgc ccggccgaac ggagatcagc acacggcagc
     3901 ttccctgctc gcgggaatgc acggacagga tcagcacgtc cttttccggc atgctgatcc
     3961 tgtcgatctt tgctcccaga agggagtttt ccagctcacg gcgcagcgcc gtcagcagta
     4021 tggcgtccag aggcattgtt ttcctccaaa atgatagcaa agagttcttc gatccgctcg
     4081 ttttgtccgc ggagatagag ccacccgaac agcgcctcca gcccggaggc ggcgtggtac
     4141 tcggcctccg tcgcagcgcg gggtgcggca tggctgtgcg cgttgcgccc gcggcggtat
     4201 acggcctcct cctcgcccgt gagatgcggg cggatctttt ccgccgcgcg cgcctgcgcc
     4261 ggggcggata cataccggat ggtctgccgg tgcagctcac cggcggtcag tttcccggag
     4321 cggcaaacct cccgccgggc aaggatctca tacacggcgt cgcccatgtg ggcgagcgcc
     4381 agcgcgctca tggcgcgcac ttcaccgtcc gaaaggatca tagacgctcc tccgcggaga
     4441 aatcgtcctt ctgctcctcg atgagctgct ccatcggcgt gcggccggac atgctcatct
     4501 cctgccactg ctcacgggtg atgagaagct ggcggggctt ggagccttca aacggcccga
     4561 cgatgccgag ctcttccatc tggtcaacca gacgtgcagc gcgggaatag ccgagcttca
     4621 gccgccgctg cagcatggag acggacgcct gcttcgtatc gagaataacg tccaccgcct
     4681 gcggcagcag ctcatcgcag ccgctgaacg gattatcgtc gagcgcggcg gaatgcccgt
     4741 cctcgtcttt ctcctccggc gcgtcctttc cggcggcggc cttttcgatc tggcggagga
     4801 tctcatcgtc gtactccggc gtcgtcccct gtttgataaa gtcgatgacc tgctcgcgct
     4861 cctcgtcgga gacgaaagcg ccctgcacac gaagcggctt tccgacgcca agcggcgcat
     4921 agagcatatc gcccatgccg atgagctttt ccgcgcc
//
LOCUS       DAWAAE010000147         4940 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_6961_length_4940_cov_2.970931, whole genome shotgun sequence.
ACCESSION   DAWAAE010000147 DAWAAE010000000
VERSION     DAWAAE010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_4940_cov_2.970931"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3401..3410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1125 a         1353 c         1406 g         1046 t
ORIGIN      
        1 atatagagat aatcacggaa ccatgtgccg agcgacatat gccagcgccg ccagaattcc
       61 gtgacgctct tcgagatgta cggataattg aagttctcca gaaactcaaa accgagcatc
      121 cgcccgagac cgatggccat atccgagtag ccggaaaaat caaagtagag ctggagagaa
      181 aaggcgagcg cgccgagcca cgctccggca aagctcagct ccggagcagc ggcatagata
      241 tcccagagcg cgccgatgtt gttggcgagc agtaccttct ttccgagacc aatggcaaaa
      301 cgctgcacgc cggaggaaaa ctgttcggag ctgagacgcc gcgtgccaag ctgggaggcc
      361 acgtccttat accggacgat cggcccggcg atcagctgcg ggaagagcgc gacaaatgtg
      421 ccgaagcgga taaagttttt ctgttcctcg gtgtcgccgc ggtatacgtc gatcggatag
      481 gacatcgtct ggaacgtgta gaaggaaatg ccgatcggca gcgtgagacc gagcgcgggg
      541 atgcttatgc cgggaatgcg gctgagtgtc gcggcgagca gatcgaagta tttgaagaaa
      601 atgagcagcg cgaggttgac aacggtattg accgtcagaa cggcccgcgc ttttttatcg
      661 tcggtcagcc tatagcgggc gatgagaagt ccggcagcat agttcagcgc gatggagaat
      721 accatcagca ggatgtacac cggctcgccc cagccgtaaa acacgagatt tacgataaaa
      781 agccagagat tccgataccg cgccggcacg atatagtata ccgccagcac gaccggcaga
      841 taaacaaaca ggaaaagaat gcttgaaaag accatagatg tacaactctc cgtaatgcgt
      901 tatctattta tcggatttct ccggggaaaa tttgccgaga ggattggcct gcgccgcaat
      961 gcgcagctcg gaattatcca catgcgtata gatttgcgtg gtgttcagat tttcatggcc
     1021 cagaagctcc tgcaaggtac gcacgtccac accgttcgag agcatcagcg ttgccgccgt
     1081 atggcgcagc ttgtgtgcgg aatacttgtc ggcgtccaga ccggcacgca ggagagattt
     1141 cttcaccatg gcgtgcagcg cttcgcgggt aatgcgtgtg cggcggctgg aaaggaacaa
     1201 agcgttccga tcaatggcgg caatatcccg gcggacgatc agataatcgt tgatcgcctg
     1261 cgccgtggcg tcgttgagat agacgacgcg ctctttgttg cccttgccga gcacgcggat
     1321 atgatcgctc cgaatgtctg agagattcat cccggcgatt tcggaaatac gaaggccaca
     1381 gttgaggaat atcgttaaaa tgcagaaatc gcgttccttg tgcatcccgt ccacgctgtt
     1441 tagtaaacgc tcgctttcct ccagcgtcag atagcggggg agagaccgct tggaacgcgg
     1501 ggagtcgaga tcctgcaaag ggttctcgtc aaggagcttg gcttttgtcg tgagatactt
     1561 ataaaagccg cggatactgg agacctttcg ggcacgggac gcggcgttca ggccgaattc
     1621 ctcgctgcgg gcgcgggtga atttggcttt gtcgcgggaa agaaacgcca gataatcata
     1681 aacctccgca agcgtaacgc gggagacaaa gtccagatcc acatcgagga tcgaaacttc
     1741 ggaaagctcc gcggcacggg gaacgagccc gcgctcgatc ttcaaaaagc ggaagaaatt
     1801 ccgcagatcg agaaaatact catcgaccgt agcgcgggag tgacccttga tcgtttcatg
     1861 gtatattaaa tagtcccgca gaatgggggg cgcttcatag cggtagtcca tagatgaacc
     1921 tcacagcaaa aaaccgccgg acaagggaag gcatttccgg ctatacataa aattaaacaa
     1981 aataaagatt ttgtttaatt taggggagtc ctttttcaaa tgaatatggc ggtaacggca
     2041 tcgcggacgt tttttgccgg caacagcttc atgtcctttg ggagctttat gtcatccttg
     2101 cagtgcgccg gtataacgca gcgcttgaat ccaagccgtg caagctcctg caggcgttga
     2161 ttgatcgacg gaacggatcg aatttctcct gtgagtccga tttcgccgat cgcggcaaga
     2221 tcttccccga taggcttgtc ccgcagactg gacgcaatgg ccagcgcgag ggcaaggtcc
     2281 gccgcgggct cgtccagctc cagaccgccg acaacattta cataggcgtc gcacccggcg
     2341 acgtgtacgc cgccgcgttt ttcaagtacg gcaagcaaaa gcatcagccg gttgaagtcc
     2401 acaccgtttg ctgtgcggcg ggactgatta gcattcgacg gtgaaagcag cgcctgaacc
     2461 tcggcgagga tcggccgcga gccctgcatt acacaggaaa cgcatgtacc gggcgcgttg
     2521 accggacggc cggacagcat aacctcggac ggattcggaa cttcacgcag ccccgtatcg
     2581 cacatttcaa atacgccgat ctcgtttgtg gaaccgaaac ggttttttgc ggcacgcaga
     2641 atgcggtaat tgagcgaacg gtcgccttca aaatacagca cgcagtccac catgtgttcc
     2701 agaaccttcg gtccggcgat cgcgccttcc ttgttgacgt ggccgatgac aaacgcggtg
     2761 atattcatgg tttttgcaag ctgcatcacc gccatggtgc agtccttcac ctggccgatg
     2821 ctgccgggcg cggaggcgat gtcgttattg tacatcgttt gtaccgagtc aatgatcaga
     2881 atatccggtt tcagatcctc cacgctctgt gtaacggcgt tgatatccgt ttctgcaagg
     2941 acgaaaagat cgttactcga tacgccgagc cgctgcgagc gcattttaag ctggcgctgg
     3001 ctttcctcac cggtcacata caggatcttc gcgtccggga tattgccgca tatttgcagc
     3061 agcagcgtgg attttccgat gccgggcgcg ccgccgacca gtacgagcga gccgcgcaca
     3121 gcgccgccgc cgagaacgcg gtccatttcc gccatgccgg tggaaaaacg aatttcttcc
     3181 tcgtcgtcaa gctgtgcaac gggcttcggg acgctccggc gcgttccggc gatctttacg
     3241 gcagcgcttt ttgccgcagc attgccggac ggcgcggagg gcgcttccac gattgtgttc
     3301 cacgcaccgc acgccgggca gcggccgaac cattttccgg tttcattgcc gcattccgtg
     3361 cagaaaaaca cacttttgac tttcataaaa cgcctttctc nnnnnnnnnn aaactggaga
     3421 tagcatgccg tcaagacgga cgcgatcttt ttctgatagt ccgcgctttg cagaagcgat
     3481 agctcctgcg gatgcgaaag gaatccgcac tcgaccagca cggcggtaca ttgaacgtgg
     3541 gacataatgt aaatgtcctt ccctgccggc accgggacgc gccggttttc cggcagcagc
     3601 gccgcggtga gccgggattg gatgagttcg gccaaagcgc ggctgggttc attctgcgcg
     3661 tataaaacct gggagccgtg cggcccggat gagggataga tgttctgatg aatgctcaaa
     3721 aaaaccggag aatccacgct ttcggcgatc tctacccgcc gccgtgtatc ccattttttc
     3781 atggcggcaa tggtttttgc ctccgacgga taggtgatct cctcggtatc gcgcgtcata
     3841 acggtatcaa cgccgagaaa agcgcataga tcccgtacgc gcagcgccac ggaaagattc
     3901 aaagcacttt ccgccgtccc gtccggcgct acagcgccgc cgtccgcgcc gccgtgaccg
     3961 ggatcaagga tcagcacagg cggcgagcct gcggcctgtg ccgacacgga tcgggtatcc
     4021 gtacccgata aaaacacaca ttgtattatg acacaaatcc aaaggaatga caaggtgaaa
     4081 agcgcggcat cttccgcgcg gacgcttcta tacagctttt taacaaacaa aatgcatcgc
     4141 ctccctcaag ctatgctatg agagcggcga tgcattttat gagcggaagg actcagcctt
     4201 ccttcaggaa tttctccagc cggtcaaggc cctctttgat ggtctcgagc gacgtggcat
     4261 acgtccagcg gacaaagttc ggcgcgtcga acgcgcagca ggatacgacc gcgacaaggc
     4321 ctttttccag aaatgccatg gcgaaatcat cggcattgtg gatcacgcgt ccgccgagcg
     4381 tgcggccgat gagcttttca agattcatca tcacataaaa agcgccgtcc ggcttcaggc
     4441 agctcacacc gtcgatctga ttcatgcgct ctacgagata attccggcgc tcctcgaaca
     4501 ccttgcgcat ttcctccacg ctgtcctgcg ggccacgcat ggcctccgcc atcgccgtct
     4561 ggttcatggc gccgatgttg ccgagcgcgt ggctcaggca gttcgtcatg actttggcaa
     4621 tgcgcttgtc gccgcaggcg gcaaagccgc agcgccagcc ggtcatggaa tagctcttgg
     4681 atacgccgtt gatgatgatc gtgtgcttct ttacttcttc gccgagtgct gcgacgctgg
     4741 tgaatttgcg tccgtcgtag cagaggcggt agtaaatctc gtcagcgatg atgtaaatat
     4801 cgtgacgcag gcagacctcc gcaagcgctt ccagctcttc cttggagtag atcatgccgg
     4861 tcgggttgga cgggttgttg agcacgagcg cctttgtttt atccgtaatg gcggcttcaa
     4921 gctgtgccgg cgtcattttg
//
LOCUS       DAWAAE010000148         4820 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7157_length_4820_cov_3.032319, whole genome shotgun sequence.
ACCESSION   DAWAAE010000148 DAWAAE010000000
VERSION     DAWAAE010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7157_length_4820_cov_3.032319"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          875 a         1457 c         1464 g         1024 t
ORIGIN      
        1 cctttacggg ccaccgggcc gaaaagctgc catggggcta taacgaaaac gatccgcgct
       61 gccggagact gaaacggacg ctgtacgata cgctggaagc ggtatatgaa tccggctttc
      121 accggttcat ctgcggcatg gccgaggggt gcgatctcta tttctgcgag gctgttctcg
      181 cgctgcgcga ggatcacccc gatataacgc ttgaagccgc cgtcccgttc gccggacagg
      241 cggagcactg gggcgcggcg cagcggcagc ggtacgaagc ccttctcgcg cgctgcgata
      301 ccgttaccgt gttgcaggag cactactccc ccggctgcat gatgaagcgc aaccggtaca
      361 tggtcgatca cgccgatctc atcatcgcct gctacgacgg taaatccggc ggcacgctca
      421 acacgctgcg ctatgccatc gagcgtgata tccagatcgt gcatctgccg ctggaaaatg
      481 tataggagca ggggcatgga ttttaaaaca atcgataagt caaactaccg ggaatgcatg
      541 gaattgcagg tcgaggaaag ccagaagtac tttgtcgcag acaatacgcg ctccctcgtt
      601 gaagccgcct atgaggacgg gctgtacacg ctcggggtat acgatggtga taccatggtc
      661 gggtttgttc tgtacgacta cgacgatata taccccggct ggtcgatgag ccggtttatg
      721 atcgcccgtc agtttcaggg caaaggttac ggcaagcggg ccatccggga gtttcttgat
      781 tatttcaaaa agaaacacgg cgcagataga ctttatatca gcgtatctct cgacaacgcc
      841 gtcgcgcgga aaatgtactc gggcctcggc tttcgggaga taaaagaaat agaatactcc
      901 tattccggcc atctttaccg tgaaatgcag atggtaaagc atctctaata ccgcttacag
      961 cacaaaacgg cgcagacagt ttccctgtcc gcgccgtttc tgcatgataa aatttgaaat
     1021 cagaagccga agttcagccc gccggtgacc ttcgccatgc tctcctcgcg ggaggtctcc
     1081 gccaccttga gcgcggagtt gaccgccgcg atgaccatgt cctgcagcat ttccacatcg
     1141 tcgggatcga cggcctcggg gtcgatcgtg agcgtgatga gctcgcgctt gccgttgacc
     1201 tccgccgtga caacgccgcc gcccgccgaa gcggagtatg ttttggcgtc cagctcctcc
     1261 tgcattttca gcatgtcctg ctgcatcttc tgtgcctgct tcataagatc gctctgtctc
     1321 atgccgccgc ccatgctcat accgccgccg aagctgccgc ctctgtatcc ttttgccata
     1381 gtttcctgtc tcctttttat tggatggtta cattgccgaa tttgccgaga aaatcaagtt
     1441 tatcctcccc ctgtgcgccg ccgctttgca gttcctccag ccggatctgc atcggcgcgc
     1501 cgcagagcgc agacaccttc tgccgtatct tgttgagcac gtccgggacg ttgacggcgt
     1561 ttttcgcaaa gcccgggagc agatgcagga tcagctcgcc cccgcggatc tcgcccgagg
     1621 actgcgtggc gtccgtcaga acgatgcgaa cgccgatagg cagctcgttt tcaagctgcg
     1681 agagcgcgta ttcccagctg acgccgcccg cggggacact gtccgccgct tccgtcaccg
     1741 ggacgctttc cgaggcttcc gtcaccggga cgctttcctc cgccggaggt gccggttttg
     1801 cttcttccgc cgggacaggc tcactcgccg tgatcggcgg cgcatatacg ggatcgctct
     1861 tcgccggagc cggcgtttcc tgtaccggcg gcgccggagg aggcgtcggg atgtcccgct
     1921 gttccggccg gggcgcggca ggtctctgca cgggagcggc cgcgcgcggc ggcgcgatct
     1981 ctccgccgcc ggagagctcc gcgagacaaa gctccgtctg caggcgcgga ttgcccgttt
     2041 cgcgcagcag tgcctggtgc ttctgcagcg tattgagaag ccagagaagg tttccgtcgc
     2101 ccgcgcggat atcgcgcaga acgttttccc cgtaagctcc ggagatcagc gtccgcccgc
     2161 ccttcggggc gagacggagc agcagcgcgt cgcggaagag cccggagagc tcgccgagga
     2221 gcgccgcggg atcctttccg tcctgccaga gcgattcaaa ctgcgtgagc gcatcctcgg
     2281 tattcccggc gagaacggcg tccgcaatgg actgtgtgcg gagcgttccg gccagtccca
     2341 tagcgttgag caccgccgcg gtgtctaccg tttccgccgc ggagcactgg tcgagcagcg
     2401 aaaggccgtc gcgcatgccg ccgtcggcaa ggcggcccag aagctgcgcc gcgtccgccg
     2461 tgaggttgat gctctcctgc tccgcaacat aggcgaggcg cttggcgatc gtgtcgccgt
     2521 cgagccgccg gaagctgtgg cgctggcagc gggagaggat cgtgggaaga actttatgga
     2581 gctccgttgt tgcgagaatg aacatgagat gctgcggcgg ctcctccaaa attttcagca
     2641 gcgcgttgaa cgccggtttg gagagcatgt gcacctcgtc gatgatgtac acgcgcttgc
     2701 gtaccgtggt cggagagaag atcgcctcgt cacgcagcat gcgcacatcg tccacgccgt
     2761 tgttggacgc cgcgtccagc tcgaccacgt cgagaacgga gccgtcctca atgccgcggc
     2821 aggcgtcgca ctcgttgcag gggtttccgt ccaccgggtg ctcgcagttg accgccttgg
     2881 cgagaatgcg cgcgcaggtc gttttgcccg tgccgcgggt gccgataaaa agataggcgt
     2941 ggctaagccg tccggtacgc acctgggtct tgagcgtttc ggtgatgaac ggctgcccca
     3001 ccacatcgtc aaaggttttc ggtcggtatt tccggtacag agcttcgtac aaaatccatc
     3061 tctccgtttt tgggaaaata gaaagcgatc cttggcaaga ctgcacacct ttccttgaat
     3121 gatgcggtat gcccggtgca acagcagccg gctcaggtcc ggcaccctca cggcacacac
     3181 agcgttccgc ttaatgctgc tcggttcccc gcctgacatg gttcacgggg ctgtgttgcg
     3241 cgagaccaga ccgtcagcgc cacttactgc cgccagacga cacatcgcat accctcggag
     3301 aggaattcat ccctgctata gcggattgca ggctacaggg caccgctggc tccccgacta
     3361 gtggagcctt gccaaagacc gcttcgttat tatatcggat gcgcggaaaa atatcaagtg
     3421 tcttttgcgt cgtgttttcg gtttcccgca tatcctgata catggaaagg gtgtgcatcg
     3481 ttatggaaca aaaaaattcc cggcagtttt tcctcggcgc aaacaccggc gtggggttcg
     3541 tctcgctcta ttcggcgttt gccgcgccgg aggacggcgc atttgtctgg tacatcaagg
     3601 gcggccccgg caacgggaaa tccacgctca tgcgccgcgc cgccgagcgc gcggaggaag
     3661 cgggactcgc cgttgaatcc attctgtgct ccggcgatcc ggattcgctc gacgggatat
     3721 acattccgga aaagaagctc gcgtatgtgg acgccacgtc tccgcatgtg caggagcccg
     3781 ccgttccggg cgcctcggga aagtatctcg atctcagcgc attttaccgt cccattccgc
     3841 cggaggaggc ggagaaaatg cggcggctct ttgctgcgta ccggcgtgag tatgcgcgcg
     3901 cctatgacat gctgcatgcg gcaaggctcg tctcccccgg cggcattccg ggcgttctta
     3961 cggcagaagc taaagcgcgc gtgcgaaaac gcgccgaagc gttcgcagaa aaggccgttc
     4021 atcgttcgga gacagaatac gaaaagcggc gttttctctc ggcctatacc tgccgcggcg
     4081 cggtgctgct ctcggagacc gccgcgtcgt tcgggcgggt gtacacgctg gataatgagc
     4141 ttgggcttgc agacgatttt ttgcaggcgg tgcttgaacg ggcgcgctcg gccggcgcgg
     4201 cgcgcatcgt ctgtcccgat ccggttgatc cggaaaagct cgcggcactg atcctgccgg
     4261 aggatggact ctcgctcgtc gcggtgtccg gcgatttccg ctttgacggc aaggccgcgc
     4321 ggcacttcct gctcgacacg gcggcggagc tttccgacgc aaagcgaaag gaagtccgcc
     4381 gcgccgccgc gctgcgccgt tcgctcgcgg tggaggcgtg ttccgcgctg cggcgggcga
     4441 aggagctgca cgacgaactg gaggcggtat atcatccgtt tgtggatttc cgcgcgctga
     4501 cgcgttttga gcagaagcat ctggaaaagg tcttttcggc ataagcgtcc gccgccgcgc
     4561 gacgcgcttt tcacgtactg ggcacagctc ttcgttaact tctggtggtc catctttttc
     4621 tttgcctggg gcctccgtct gaccgccttt ttctggctcc ttctgctgct cgttctcgct
     4681 gcggtaatgg cgttccggtt cggaaatatc cgtcccgacg ccggaaaact gaacattccg
     4741 tatctcctgt ggctggtttt cgccggcgtg ttgaatatat cggtatacat tttaaacaag
     4801 tgaaacgttt ccgaatggcc
//
LOCUS       DAWAAE010000149         4816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7162_length_4816_cov_2.953371, whole genome shotgun sequence.
ACCESSION   DAWAAE010000149 DAWAAE010000000
VERSION     DAWAAE010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_4816_cov_2.953371"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          884 a         1529 c         1492 g          911 t
ORIGIN      
        1 gtgagcctct ttgacgcctt cggctaaatg taaaggagag atagaataat gaaactgatg
       61 atcgcgtccg acctgcacgg ctcggcgtac tatacgcagg cgctgctgcg ccgcatggag
      121 gatgaaaagc ccgcaaagct cgtcctgctt ggcgacatcc tctaccacgg cccgcgcaat
      181 gccctcccgc gcgattacga cacgaaagcg tgcgctgcca tgctcaacgc gctcgaaaaa
      241 gcgccgcttt gcatccgcgg caactgcgac ggggaagtgg accagatggt attgaatttc
      301 cccgtgctcg cggacttcgc cgccgtgttc gcggacgggt ataccctcta tctcacgcac
      361 ggccaccatc tcgacgaggc gtccaaagcc gccgcccccg gcgacatcct gctctacgga
      421 catacccatg tcccggcgtt cgagcagaag aacggaaatt attatgtaaa ccccggctcc
      481 gtatccatcc cgaaggaagg aagccgccac agctatatgc tctgggaaaa cggcgttttc
      541 acatggaagg atgtggagac cggcgagatc tggcgcacgg agcgcataga gaagtgagcg
      601 gcgcgccgga ggcggtgcgc gcgcggctcc ttgcgctgca agacgagaaa aacgcgccct
      661 tcatggcaaa gctcgtcccg acgctcccgc cggagagcgt tctcggcgtg cgtatgccgg
      721 attgccgctc gctcgcccga gagctttgcc gggaacccga catcggcctc tttctcgccg
      781 atctgccgca ccggtatttg gacgagaaca acctccacgc gctgatttta aacgaggaaa
      841 aggactatgc cgccgccgtc gccgcgctcg acgcctttct gccctacgtt gacaactggg
      901 cgacctgcga cgcgctgcgc ccaaaggctt tcaaaaagcg ccctccggct ttgccggacg
      961 agtgccgccg ctggataaaa agcggcgaga tctatgctgt ccgcttcggc atcgaaatgc
     1021 ttatgacgca ctatctcgac gcggatttcc gcccggaata tctcaaagaa gtttccataa
     1081 ttaaaagtga agaatattat gtaaacatga tgatcgcctg gtactttgcc acagccctcg
     1141 ccaagcagta cgaagcggcg gttccgtgca tcgagcagcg ccgcctcgcc ccatggacgc
     1201 acaacaaaac catccgcaag gccatagaga gctaccgtat caccgacgag cagaaggcct
     1261 atctccgcac gctcgccata cgagaataac aaacgcccga aagagcaaaa ctctttcggg
     1321 cttcatttta tcggttgggg cttgccagat acccctcgag caggagggtt gccgctactg
     1381 cgtccacgtt ggcgcggtgc tttttttctt tttttccggc ggcgtggagg atggcgtgcg
     1441 cctcaatggt gctgcgccgc tcgtcccaga gtatgaccgg caggccggag gcctcctcca
     1501 gcaatgcctt gaactgccgc gccttttccg cgcgcgggcc ctccgtgccg tccatgttct
     1561 tcggcaggcc gagcacgaac ttttcggcgt cgcgctcacg ggcgaaaaag gcgagccgct
     1621 gcgcgagctt ttcggcgttc cattcctgaa tggtgagcgc ttctcccgca agcgtgccgg
     1681 tgagatcgga caccgcaacg ccggtgcggg cgtcgccgta gtctatggcg atgatccgtt
     1741 tcatggcagg acctcctgta tatgtctttt tccttactat accacaccgc agcgccccgg
     1801 cgcaatgccc tctccgttcg acggcttttg caggacgctt ctgctatgct cttacccgaa
     1861 aaaacatttc ccgcggtttt ccgtggggaa gggaggaaag agcatgaagc gccggatcgc
     1921 ctgtctcacc gtatgtctgc ttgcggttct gctgccgctg cgcgcgctgg ctgcggagga
     1981 gaccgccgcc gtacagacgc gcgccgggga ctggacgccc tcacgcagcg ccatgccggg
     2041 cgaagagatg catttccgcg tctcgctcac gctgcgtacc ggcgagagct ggacggtacg
     2101 cagcgtgttt tcccccggcg tcgagtttct cgcgctcacg gcggtccgcg ccgacggaga
     2161 gagcgtcaac gcctcgtact atacgctcgt caccggcgcg cgctcgaaag agagcgcctt
     2221 tgcgctccat ctcgcggcgc gcttttccgc gcgggagagc gtctgcctga cggtcgagtg
     2281 gacggcgcgg ctgggcgacg gctgcgttac cggtgcggaa ggaaaccgcg tatttctcac
     2341 cgcggtgagc gagagcggcc agaccgcagc gagcggcagc gcggcggttt tcgcctacgg
     2401 cttttccgtt ttccgcggtg tgcgctttgc cgatgcgcgc gtggcggatc atccgctgcc
     2461 gtccgcctgc ttccggctct attatgacgc cgcggcgcgc gagccggtgg cgttccgggg
     2521 gaacgggggc gcgtatctcg cgtgcgcgct cggctgcggc cacacgcgcc atacctacat
     2581 cctgcgcacc gcggaggacg ggtacgtccg catggcggga cttcccgccg gaacgtatta
     2641 tttgcaggag tcgcgcccgc cgtccgggta cggctgcgct gccgacatgc tgcgcgtgac
     2701 gctcacggag gacggagcgt tggaaaccga cgcgtccgaa gcgtccggca gcacggtcct
     2761 tctgcttgaa acggcgcagg aatcgctgcc ggagaacaaa acgctgacct tttaccgccg
     2821 cggctgcggc gtgatggcca tgctgttcac caccgttctg tgcggaaagc ggcggtattg
     2881 gtgaatcatt ttttctcctt gggcttgaag agcacggcgg cgagataccc gaagaatatc
     2941 gccgcggcga tgccgcccgc ggaggccgtc agcccgccgg tgaacgcgcc gagaaagccc
     3001 ttctccgcca cggccttttc cacgcccttg gcaagcgtgc tgccgaagcc cgtgagcggg
     3061 acgctcgcgc cggcgcccgc ccactcgatg agcggagcgt acacgcccac ggcgtgcagc
     3121 acgacgccga gcacgacata gcacgtcaga atgcgcgccg gggtgagctt cgtgcggtca
     3181 atgagcacct gcccgagcgc gcacagcgcc ccgccgacga gaaacgcctt gagataatct
     3241 gtccacatag tccttacccc ctatcaacgt ccagaactac ggcatgacag atgccgggga
     3301 tgctctcgcc ctgctgcgat gttgtcggcg acatgagcgc gccggtcgcg gcaaagagca
     3361 cacgcggcca gtcgccgcag gtcatgcgct ggagaatgtg cccgcagagc accgatgcgc
     3421 tgcacccgca gccgctgccg ccggcgtgta cgtcctgcgt ctccggatta aaaatgagca
     3481 tgccgcagtc cgtggagcat acgccgagat ccacgccgtc ccggagaaag agatcggtca
     3541 agatttcgtg cccgatcttt ccgagatcgc cggtgatgat cgcggcatag tccgccgggg
     3601 tgcgcccggt gtcggcaaag tgcgcgcgga gcgtttcgta tgctgccgga gccattgccg
     3661 cacccatgtt gttcgcgtcc gtcacgcccg cgtcgcggat gcgcccggtc gtgacgtgcg
     3721 tcacataagg gccttcgccc tcgctcgtga gcagcagcgc gcccgcgccg gtcacggtcc
     3781 actgcgccgt cggcgtgcgc tgcccgccgt attcaagcgg gagccggaac tgccgctccg
     3841 ccgagcagaa gtgtgagctc gtcatggcgc acacggtgtc ggcatagccg ccgtcgatcg
     3901 tcatcgcggc gagcgacagg ctttccgcca tcgtggagca cgcgccgtaa acgccgaagt
     3961 agggaatggg gagatcgcgc gcggcgaacg ttgagccggc gcactggttg agaaggtctc
     4021 ctgcgaacag atactgaatg tccgagggcg gaatcctcgc cttgtcgcag cagagcgcaa
     4081 agcacttgga gagcatcacg ctctccgcct tctcccagct cgactggcca aagtaggaat
     4141 cgtcgctgat atagtcgaac catttgcgca gggggccttc gccctccttc tgcccgacga
     4201 cgcagctgcc cgccgcaatg gagggcgggt tcgtgaaggc gaccgtctgc cgcccgagcc
     4261 gcttgttttt cagaaaaacc accgtctttc ccttttttca tagcgtaccc cgaaaaagca
     4321 aaaaaatccc tccggaatga accggaggga gaaagatatt tgatttacgt gcgcccgacg
     4381 gactgatagt tccagctgag cgagttttcg cgcggcatca taaagttatc ggtgtacatc
     4441 tggagaagcc agtagttcat ctcgatctcc gggtcgcaga tgtagatctt gccgtcgagc
     4501 gtgatctccg tccaggcgtg cggctggttt tctatgccga tgctgccgga ataggtgcgg
     4561 gcgttgtacc cgagcccgcg ggcgagcgcg cagaacgctc cggcgtagca gtagcagttg
     4621 cccttgttcg tgcggaagat cgtgagtgcc tcgtcaatgt cccagccggt cgcgccggag
     4681 tcgtagtagt tgcgcgtgag atactggaag tcgtttttca catggtagta gatcgctttg
     4741 agatcgtcga gccgcgcggc gtccggcgtc atgaagtcgg agagcgtctg cgcgacgtag
     4801 tcatcgagct ctgcgc
//
LOCUS       DAWAAE010000150         4755 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7282_length_4755_cov_3.038085, whole genome shotgun sequence.
ACCESSION   DAWAAE010000150 DAWAAE010000000
VERSION     DAWAAE010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_4755_cov_3.038085"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4171..4180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a         1417 c         1373 g          923 t
ORIGIN      
        1 gactatgccc gcatcttcaa aagcgagatc atcaccgacc tcatcaaggt gctcggctgc
       61 ggcgtggagg tcgaaaagca cggcaaggag ctcaacaact tctcgctgga aaacctccgc
      121 tggaacaagg tcgtcatctg taccgacgcc gacgtggacg gctaccagat ccgcacgctc
      181 attctcacga tgctctggcg gcttgtgccg acgctcatcc gcgagggtta cgtctatatc
      241 gccgaatcgc cgctgtatga gatccaggca aaagggaaga cgtggttcgc gtactccgac
      301 cgggaaaagg cagagatcgt ggagtccatc ggcaatgcga agatcgtcat caaccgcagc
      361 aaaggtctcg gcgagaacga cccggagatg atgtggctca ccacaatgaa ccccgagacc
      421 cggcggctca tccgcgtcat gcccgaggac gccgacgaaa cggcgcgcgt tttcgatctt
      481 ctgctcggcg acaatctcgc cggacgcaag gagcacatcg ccgaggtcgg tagccggtat
      541 ctcgatctgg cagacctttc ataaattatg gaaaccgaat tatgtaattt atattatgta
      601 aattgattat gcaaacaata ttacgtaaat ataattatgt aaacttattt tgaccgtata
      661 aggaagcata tatggcaaga aagaaggaaa acccaacgcc gcgccacgcg acggccgagg
      721 aacagaacgt aatggggctg cgctccgccg ttgtggagca gcccgtcacc gaaacgctcg
      781 agaacaacta catgccctat gcgatgagcg tcatcgtctc gcgcgccatc ccggagatcg
      841 acggctttaa gccgagccac cgaaagcttt tgtactgcat gtacaaaatg gggcttctca
      901 ccggcgcgcg cacgaagtcg gcgaacatcg tcggcgcgac gatgcactat aacccgcacg
      961 gcgactccgc catctatgat acgatggtgc gtctctccaa ggggtacggt gcgctgctca
     1021 cgccgtttgt cgattccaag ggcaacttcg gcaaggtgta ctcgcgcgat atggcgtggg
     1081 cggcctcgcg ctatacggag gcgaaactcg cgcccatctg cgcggagatc ttccgcgata
     1141 tcgacaagga cacggtggat tttgtcccga actacgataa ctccaccacg gagccgacgc
     1201 tccttccgac cacgttcccg aacgttctcg tctcggcaaa catgggcatc gccgtcggca
     1261 tggcgtcgaa catctgcggg ttcaatctcg gtgaggtgtg cgaaacgacg atcgcgctgc
     1321 tgcgcgaccc ggaacataat attctgacaa cgctccctgc gccggatttc ccgaccggcg
     1381 gcgagatcgt gtgcgatccc ggcgagatgg aagcgctcta taagaccggc cgcggcggtg
     1441 tgaaggtccg cgcccgctgg cgcttcgatc ccaaggagcg catcattgag atctacgaga
     1501 tcccgtacag cacgaccgtc gaggcgatca tcgacaaggt cgccgagctc gtcaaggccg
     1561 gcaagctccg ggagatcagc gacatgcgcg acgagaccga cctcaacggt ctcaagctcg
     1621 ccatcgatgt caagcgcgat gtggagcccg aaaagctcat gcagaagctc ttccgcatga
     1681 cgccgctgca ggacagcttt gcctgcaact tcaacattct gctcggcggc acgccgcgcg
     1741 tgtgcggcgt cggcgaggtt ctggacgaat ggatcgcctg gcggctggaa tgcgtccggc
     1801 ggcgggtata ctttgacgta tcgaagaaga aggaaaagct ccatcttctc aacggtctca
     1861 aaaagatcct gctcgacatc gaccgcgcca tctccatcat ccgcaacacc gagcgcgaga
     1921 gcgacgttgt gccgaatctt atgatcggct tcggcatcga ccaggtacag gccgaatatg
     1981 tcgccgagat caagctgcgc aacatcaacc gcgaatacat cctcaagcgc accgaggaga
     2041 ccgacgagct tgaacgcgag atcgccgagc tggaggatac gctcgcgagc cgccgcaagc
     2101 tgcgcgcgat catcatttcc gagctgcagc aggtgatgaa gaaatatccc tcgccgcgcc
     2161 gcagcgtcat cacctatgcc gccgacgcgc ccgaagcacc ggaggaggat gacgtggagg
     2221 actacccggt caacctgttc ctttcgcgcg aagggtattt caaaaagatc accccgctct
     2281 cgctccgtat gggcggcgag cagaagtaca aggagaacga cgggccgagc cagagcttcg
     2341 agacgacgaa ccgcgccgaa atgctcgtat tcaccgacaa ggcgcaggtc tacaagtgcc
     2401 gcgcggcgga ctttgccgat acgaaggcct cgcagctcgg cgtatatctg cccggccatc
     2461 tcggcatgga cgacggcgag aacgtattct ctctcgtcct gcccggcgat taccgcggcg
     2521 aggggctgtt cttctttgaa aacggcaaat gtgcccgcgt gccgctcgag agctacgcca
     2581 ccaagacgaa ccgcaagcgg ctcaccggtg cttactgcga caagagcccg ctccgtgcgc
     2641 tgctcgtcct cacggaggag cagaacgtgg cggtgttttc cacggaggga cgcgcgcttg
     2701 tattcaacac ggcgctgctc gcgcccaaga cctcccgcgc aacgcagggc gtggcggtca
     2761 tgacgctcaa gccgaagttc cgactcgagc gcgccgcgtt cctcgccgat acgaccatcc
     2821 agaatcttgc gcgctaccgc atgcgcagca tccccgccgc cggcgcgctc ctccgcgagg
     2881 aggaccgggg cgaacggcag atgacgctgc tcgacggcgc ggaggaaaat acgaaataat
     2941 aaagaaagaa accgattgag ctatggaagc aaaacttcac ggaaaactct ggtttgatgt
     3001 tctgaagatc cttgtggggt cgtcccttgt cgcgatctcg ttccgctatc tcacattccc
     3061 gaacagcatc gtttccggcg gtgtgaccgg tatcgcgcag attgtgaatc tgctgaccgg
     3121 acttccggtc ggcgcgatca cggtggcgat caatgcgccg ctcttcatct gggcgtggaa
     3181 gcagcttggc aagcgttttg tcggccttac gcttctctgc atgctgacaa gctcgctgct
     3241 catcgacctt cttgcgcatt tcccctatac cgtcacggaa gagccgatgc tcgcggcggt
     3301 ctatggcgga ctcgtttccg gtgcgggcta tggtctctgc tatacgtccg gcgcaacggc
     3361 cggcggcacg gacatcccgt cccgtatgct tcggcgccgg tacccgcata tcaatatgac
     3421 gacgtttatc ctctttttta acctcgtcat cattgtgaca ttcgcaattt tgttccaccg
     3481 gttcgacagc tgcatgtata cccttatttg catgtatatc accaacaaag tcgaatcgct
     3541 tctcctctat ggcccggtca actcccggct gtgctatatt atctccgaca aaagcgccga
     3601 tctcagcagc gccatcaccg gacggctgca ccgcggcgta acgctgctcg acggccacgg
     3661 cgcgtggtcc ggcaagagcg agcgtgtcat tatgtgcgcc gtgaaacccg gtcagctggg
     3721 gcccctccgc cgcatggtac gcgagatcga cccgaaatcg ttcctcattg taggcgatgc
     3781 ccgcacgatc tacggtcacg gctttgagag catagaggag gattgagcgc tatggcggat
     3841 ctggaaaaga cgggaaagaa tctggaaggc cggggctttc gcgttcatcg cttcgcttcc
     3901 ggcgcggaag cggcggagtt tctcgtgcag acgctgcacg gcaccagcat cggcatcggc
     3961 ggcagcgtga cgatcgatac gctcggcgtc tatgaccgta tctgcgggag caacgaagta
     4021 ttttggcact ggaagaacca tgcgccggaa acgagagagc gggcagggaa ggcggagacc
     4081 tatctctgca gcgccaacgg cgtgagcgag aatggcgaga tcgtcaacat cgacggcttc
     4141 ggcaaccgcg tggccggtac gatctacggg nnnnnnnnnn ccgctcaatg cccggcggct
     4201 gaaccgccat acgccgtgcg ccgtgggcga gccccgctgc cacgactgcc gcagcccgga
     4261 aaaggtctgc ggcgtgatga cggtgttctt catgccgccg acgtccatca aagagtttca
     4321 tgtgattctc gttgacgaga ccctcggtta ctgatttccc ggaatttttc accccggcac
     4381 gcatatactg gctcgtatat gcatggaggt gttgacatgg gaccggatat cgatgatctc
     4441 attttcggca gcggcgatac cgcgccgagc gacaggtaaa gagataaaga cgaaaaagcc
     4501 cttccgcggc cgcggaaggg cttttgaaca ttttggggcg cacaacgagc gggactttcg
     4561 tatcgcctgc ggaaacacgc cgtccggctg ctgctctttt ttgcttgcat tttcccgttc
     4621 ttctctttgt ttttaaataa acgatctgcc gggatataaa gaaaacaccg gaatcgtcca
     4681 gagacgattc cggtgaactt ttggagcggg caacgagatt cgaactcgct acctccacct
     4741 tggcaaggtg gcgct
//
LOCUS       DAWAAE010000151         4728 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7333_length_4728_cov_2.866467, whole genome shotgun sequence.
ACCESSION   DAWAAE010000151 DAWAAE010000000
VERSION     DAWAAE010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7333_length_4728_cov_2.866467"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          877 a         1399 c         1437 g         1015 t
ORIGIN      
        1 ggagcttcgc agcagtctgg cggaatccgc cgaggacgtc atgagcgact cgaacgtcta
       61 taacatcctg ctcatcggcg cggacggacg gacggaagag cttgagcgaa gcgacgcgat
      121 gatcctgctc tccatcaaca aaacgacgaa gaccatgtgg ctgacctccc tcatgcgcga
      181 cacgcaggtg gagatcgccg actggggcat cggccatctg aactgggcaa cgcgcttcgg
      241 cggcgtggat atgctcatca acacgatcga gagcacgaga aactttgctg tcaacgtgga
      301 taactgggcg atggtggact ttctggactt cgccaagatc gcctccatgg taggccccat
      361 cacggttacc gttaccaagg aggaggccgc ggatatcaac ctcctcatcc gcgaggcgtg
      421 ccagctcaac gacacctatt acggcaccga tgtgccgcgc tgctatttcc cgaccgatca
      481 ggacggcacc tataccatca gcgacggctt ccagatcctc ggctactgcc gcgagcgcca
      541 cgtcggcggc gacaccggcc gcagccagaa gcagcgcgac gctctcatgc agatgtgggg
      601 caacgtgaag aagatgagcc tcgccgagca gtacgcgctc gcgaaggata tcatgtccat
      661 catcagcacc gaccttacca tgggccagtg cgcctcgctg ctgctgcagg cgcccgattt
      721 tctgacctac gatatccaga tgcagcagtg tccgacgccc ggctcgttct ggcgcgggct
      781 ggatgcgaac ggcctttcca tttataacgc ggactttgtg ataaaccgta atttcctgcg
      841 cgcgacgatc tacggcgagc cgatgtccgc cgcggatctc acaagctact ggtcaggcaa
      901 tgcggtacag gtctactatc cgccggagag cgacaccggc gacgagaacg gctgagctca
      961 gccggaataa tgagccggac ggcagaagga agttcctacg ccgtccggtt tttttcggga
     1021 acaaagggaa atctaatttc gtcatagaat catccatctg gaatcatcca cctgaaatta
     1081 cttgaaattt aaaggagaaa aatccatgaa aagaatcgta tccatccttc tctgcgcagt
     1141 gctgctcctc tctttggcgg cctgcggaac gtctgctccg gcggacacgg acgacaatgc
     1201 cccggcaagc gcgctcgaac tgctggaaac cgtctggaac agctatagcg acgatgaaaa
     1261 attccccgcc gccggcggcg actactccga agaaaacatg cgcgacggcg cgcccggcaa
     1321 cttcagcatg gacgatgcca atcttctgga ttcctcgctg ggctttcccg cggcggaagc
     1381 cggaaagatc gacggcgcgg cctctcttgt gcacatgatg aatctcaaca cgttcacctg
     1441 cggcgcgttc catgtaaagg acagcggcga tgtggccgcg ctgtgcgcct cgcttcgtga
     1501 aaacattctc gaccgccact ggatgtgcgg ttttcccgat aagctcgtga tcgtcacggt
     1561 gggggactat gtcgtatccg tgtacggcta caccgatctt gtggacacgt tcaccgccaa
     1621 gctcgccgcg gcgtacccga gcgccaaaac cgtgagcgac gacccgatcg tgtaagcgcc
     1681 gatgctcttt tccggtatcc cgttcctctt ttactttctc ccgtgcgtgc tgctcgtcta
     1741 cttcatcgtg ccgcaaaaag ggagaaacgc cgttctgctc gccgccagcc tcttttttta
     1801 cggctggggc gagccgaaat tcctgctctt catggtgttt tccatcgtgc aggggtatgt
     1861 ctttgcgcgc ctgatcgggc gggggagaag gaaaaagctc tttctcacgc tctcgctcgt
     1921 gctgagcttt gccctgctcg gctgctgcaa gtacgcggat ttctttatcg aaaacttcaa
     1981 cgccgtgacg gggctatcgc tcccgctttt gaagatcgcc ctgccgatcg gcatcagctt
     2041 ctatacgttt cagatcgcca gctatgagat cgacgtatac cgcggcgatg tggcggcgca
     2101 gcataatttt atcgattttg ccgcctatgt tgccatgttc ccgcagctca tcgcgggccc
     2161 gatcgtgcgc taccgcgata tcgcgcccca gctgaaggag cggacacact cgctcgaaac
     2221 cgcggcctcg ggcgcttcgc gcttcgcggt cggtctcggg aagaaggtgc tcgttgcgaa
     2281 cgtccttgcg cagctcgtct ccgcgtataa ggcttctgcc gagcagacgg cgctctatgc
     2341 ctggctgtac gcgatcgcct tcacgctcca gatctatttc gatttctccg gctacagcga
     2401 tatggccatc gggctgggcc gcatcttcgg cttttcgttc ccggaaaact tccgctatcc
     2461 gtatatcgca aagagcatca cggagttctg gcggcgctgg cacatgtcgc tcggcacatg
     2521 gttccgggac tatctctata tcccgctcgg cggcagccgc tgctcccgca tccggcatat
     2581 tttcaacatc ctcgtcgtct ggatggccac cggcttctgg cacggcgcgg cgtggaattt
     2641 tgttttctgg gggcttttct atgcagtgct gctcatggca gaaaagttct tcctccttcc
     2701 ggcgctgaaa aaggggagag ttcttccgca cgtctatgtg cttctcgccg taacgctcgg
     2761 ctttgtgctc tttgacgcgg cgtctctgaa ggatgcgctg catcagatcg gtacgctctt
     2821 cggcgcggga agggcttcgg ggctggggac agaggctctc tatatgctcc ggagctacgg
     2881 cgtcgtgctc gcgctcgccg tgctcggcgc aacgccgttc ccggcgatgc tttggaaaaa
     2941 ggcgcaggag acaaagtccc tcgcgccggt gctcacggtc gcagagccgc tctgcacgct
     3001 cgcgctgctc gcgctctgca ccgcgtttct ggtggacggg tcgtttaacc cgttcctcta
     3061 tttccggttt tgaggaggtg cggcatgaag aagatccgaa atattctcac gcttgcgctc
     3121 tcgggactct ttcttgcggg ctttctgctc gcggcgatcc tgacgccgga cagggccgac
     3181 tcgctctccg agcggcgaaa gctcgcccag cgcccggcgc tgcgcgcctc ggcggtggcg
     3241 gacgggacgt atatgaccgc ctttgaaaag tatacgctcg accagttccc gctgcgggac
     3301 agcttccgcg cgctcaaggc atttttctcc ggggatatcc tgcggcagct cgataataac
     3361 ggcgtatatg cagccgacgg gcatctcgca aagctcgact atccggtgaa taccgatctc
     3421 gtccggcacg cggcggagcg gttttccgcc gtatatgaaa cgtattttgc caatgcaggc
     3481 gcgaacgttt actatagcgt tgtgccggat aagaactatt tccttgcgga gcaaaacggc
     3541 tatcccgcca tggactatga gcttctcacc gatacgatgc gcgccgggat gacgtttgcg
     3601 gagtatatcg atattttcga taccctctcg ctcgatatgt actaccgcac cgatacccat
     3661 tggcggcagg aaacgctggg agaaacggcg gcggcgatcg ccgcggcgat gggcgcttcg
     3721 cttccggcgg aatatgagac cgtggatgtg tcctcggcgt ttcgcggcgt gtacgccggg
     3781 cagtacgccc ttcccgttga accggacgcg ctgcgctatc tcacgaacgg cacgatcgcg
     3841 tcgcttcgcg tgtacgacta cgaaacgggc gcggagattc ctgtctacga tcttaccctc
     3901 gccgcggggc gcgacggata cgaaatgttc ctcggcggga cgaaagcact gctcacgatc
     3961 gaaaacccca acgctgaaac ggaccgggag ctggtgatct tccgcgattc gttcggcagc
     4021 agcattgccc cgctgctcgc gcagggctat gccaaggtca cggtcgtgga catccggtat
     4081 atggcgcctg cccttgccgg acggttcgtg gatctctcgc aggcggacga cgtgctcttc
     4141 ctttacagca ccggcgttct caaccacagc gaaacgatcc agtaatagaa catgaagaaa
     4201 gagagcattg cagttcaaaa cctgcaatgc tctcttttgg cttatttctt acttactgtt
     4261 gttttttgcg gcggaacaga aggaacagga tcagcaaaat gacggcggtg gaaatgccga
     4321 tgaccgccgg ggctgcggaa gaggtcctgc cgctcgcggg agaggagggc gcgctgtcgg
     4381 cgctttcggt tttgccggcc tcgctttcgg aaacgtcggt gccgggcaca tcagaagcgg
     4441 gggcgtctgc cgggatatcc gccggggcgg agacgtccgg cgagaccgtg acgccggagg
     4501 cgggcgaagc gcttgccgag gcagagggtt tgggcgtggc tgtcgctttc ggcgctggtg
     4561 tcggcgtggc ggtgggtttc gccgtcggcg tcggtgtggg cgccggcgtc ggggtaggag
     4621 tcggcgtcgg cgttggagtg ggcgtcggag taggtgtcgg ggtaggtgtc ggagtgggcg
     4681 ccgtcgtcgg agtaggtgtc ggggtaggtg tcagagttgg cgtcggag
//
LOCUS       DAWAAE010000152         4677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7414_length_4677_cov_2.821506, whole genome shotgun sequence.
ACCESSION   DAWAAE010000152 DAWAAE010000000
VERSION     DAWAAE010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7414_length_4677_cov_2.821506"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1692..1701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2993..3002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          954 a         1372 c         1428 g          903 t
ORIGIN      
        1 gtgcagatgc gtgccgacga gcgagacgag agcgcccgag cgtgtcacgg cggtgagacg
       61 gccttcgtcc tccggcagcg gcagagggcg gatcacggtg ccgggcgcgg ggacaagact
      121 ggagagcacc gtgagcggga cggcaaagcg cagcgcgagc gccgcgctgc gttcgtgcag
      181 cacctgcgag ccgagcgagg cgagccggag catttcctcg ctgtccacgg cgctcagccg
      241 ccgcgcctcc ggaatgagcc ggggatcggc ggtgaatacg ccctccacgt cggtatagat
      301 gcggcactcg tccgcgcgca gcgccgcggc cgcggccacg gcggtcgtat ccgagccacc
      361 gcgcccgagc gtcgtgatgt cgtttccggg gctcacgccc tgaaaaccgg cgatgaggac
      421 gaccttcccc tcgtccagct cacggcgcac gcgctccatc gagagacgac ggatgtgcgc
      481 gtcgccgtat gtctcatccg tgccgacgcc tgcctgccag ccggtgagcg acacggcggg
      541 aaggcccatt tcctcaagcg tcatcgccat gagcgcgacg cttgcctgct cgccggtggc
      601 gagcagcatg tcgagctcgc gctttgaggg gctgcggctg tagtgcgcgg cggtagataa
      661 aagcgcgtcc gtcgtttttc cctgcgccga gagcaccgcc gcgacggagt gcccggcgcg
      721 caccgtatcg gcgatgaggc gcgccgcgca gcggacgcgc tcgaccccgg cgagcgagct
      781 tccgccgaat ttttccacga acagcatatc gataaaacct ccggaatggc aaaatgggat
      841 ttgacactcc attgtattcg cccgccgcga tgcgcgccat tttggacaat aaaaaaggct
      901 cccttgggta aagggaacct ttcatgggca gagcatagcc ctgcccatgc gaggggaaat
      961 aggatcgtta ttggctccct tgtgtaaagg gagctgtcag cgaagctgac tgagggattg
     1021 taacctctgg aaaacgacaa tccttccgtc ttttgccttc ggcaaaatcc acctcccttt
     1081 gcacaaggga ggctttaaat aggtcttatc ccgctaaaat ctcggcgcga atgacaccgt
     1141 cggcggcgac gaggcggttc atgagcgttt ccatgctctc gctcattccg gcggtctcgg
     1201 cgctgatggt gatcgtggcg cagccgctca ccggaatgct ctgattgatc gtgaggatat
     1261 tcgcgccgca ctgggcgaac acgctcagca ccgccgagag cagtcccgtc tggtcgcgca
     1321 gcatcacctg gaacgtcaga atgtggccgt ggcgcagatc ctgaaacgcc gtgatcgagt
     1381 ccttatactt ataaaatgcg ctccggctga tgccgacgcg gttgaccgcg tcgccgaccg
     1441 tgctgcactc gccggtctgc agaagctcct tcgcttccat gaccttgaga aagatctccg
     1501 gcagcatgtc cgcctccacc agatagtagg tggggagatt tcggttcgcc atatgtgtcc
     1561 ctccgtgaaa agcatttgtt ttcggtgtgc aaatcatatc atccgcggcg gaaaaatgca
     1621 agtgcaattt ccgtgcaggg cggacattga aaaattgcaa aaaccgtgta taatactttt
     1681 attcattgaa annnnnnnnn ntcagccaga cgctcatgca gggctacttc acaatgatca
     1741 tgctcgttga tcttgccgag tgcgaaatgc cgttcgatat gctctcgcag catcttcgct
     1801 ccgtcggcga ggcgcgcggg cttgcggtgc acgtgcagcg gcaggatatt ttcgacgcca
     1861 tgcaccggat ctgaggaggg caccatgagc tatttcctca ataccgacga gatcgtccag
     1921 accatccgga tgatcgagca ggagcatctt gacgtgcgca cgatcacgct ggggctgagc
     1981 cttctcgact gcgccaccgg cgatatcaag agaaccgcgg ataaggtcta cgaaaagatc
     2041 tgccgcgagg cgcacgacct tgtccccgtg ggcgaggcca tcgagcgcga attcggcatc
     2101 cccattgtga ataagcgcgt gtccgttacg cctgtggcgc tcatcgcggg cgggtgcggg
     2161 gatgaccttg tccccatcgc ggaggcaatg gaccgcgccg cgcgcgaggt cggcgtgaac
     2221 tacatcggcg gcttttccgc ccttgtgcag aagggcttca cgaccggcga ccgcagtctc
     2281 atcgcctcca ttcccgaggc gcttgcacgc accgaacgcg tctgcgcgag cgtgaacgtc
     2341 ggctcgacgc gcgcgggcat caacatggac gctgtggcgg agatgggacg cgtcatcaaa
     2401 aagtgcgccg cgctcaccgc cgatacggac ggactcgcgt gctccaaact cgtcgttttc
     2461 tgcaacgcgg tggaggataa cccgtttatg gcgggcgcgt tccacggcgc gggcgaggcc
     2521 gaaagcgcgg tgaacgtcgg cgtttccggc cccggcgtcg tctatcaggc gctgcaggag
     2581 tgcaagggcg agagctttga cgtcgtggcc gagacgatca agcgcacggc gtttaaggtc
     2641 acgcgcatgg gtcagctcgt cgcgcgggag gcgtcccggc ggctcggcgt gccgttcggc
     2701 atcgtcgatc tctcgcttgc gccgaccccg gcggtgggcg acagcgtggc gcgcattctc
     2761 gaaaccatgg gactggaggt ctgcggcacc cacggcacga ctgccgcgct cgccctgctg
     2821 aacgacgcgg tgaaaaaggg cggcgtgatg gcctccggcc acgtcggcgg gctctccggc
     2881 gcgtttatcc cggtctcgga ggacgagggc atgatcgccg cggcgcgcga ggggacgctc
     2941 acactcgata aactcgaggc gatgacgtgc gtgtgctccg tggggctcga cannnnnnnn
     3001 nngtcatgcc ggtgcacgcg gcgtcgagcg cggaattcat cgcccgcggc ggccgcatcc
     3061 ccgccccgct ccactcgctg aggaactaat aaagataaag tgatcccgct gcttcggcag
     3121 cgggattttt atgctcgaac gttgtctttt ttgtgagcat acgatataat aaaatattac
     3181 aaaaccgtcg gattgtggca cacacggcgg acatttgaaa gatataggtg aacaacatgg
     3241 taaatatccg caaactggaa tccgatctct gggaatccgc cgaccttctg cgctccggct
     3301 ccaagctgac atccaaccag tactgcatgc cggtactcgg gctgattttt cttcgatacg
     3361 cctacagccg tttcaagctg gtggaagaag agatactgaa aaaccgtccg tcccgcggcg
     3421 gccgcgtgat gcccgtggag gccaacgatt ttgtggcgaa aagcgcgctc tttctcccgc
     3481 gggaagcgca gtacagctat ctgctgagcc tgccggaaaa catcgcgaaa gccgggatca
     3541 agaataaaga cggacacacc atgaacagcc tcggcgaagt ggtgaacaat gccatgtccc
     3601 tgatcgagga acagagcgag cagctcaccg gcgtgctgcc gaaaacctat accgactttt
     3661 ccgacgagat cctctccgaa ctgctccgca ttttcaataa cagcgcgctc gacgaagtcg
     3721 gcggcgatgt tatcgggcgt atctacgaat acttcctcaa taaatttgcc aagaacatcg
     3781 cctcggatga cggtgtgttt ttcacgccca aatctctggt aaaaatgatc gtcaacgtgc
     3841 tggaacccaa gggcggcgtg ctgcttgacc ctgcctgcgg cagcggcggc atgttcatcc
     3901 agtccggcga ttttgtcaat caggcgggca tgaacgccaa cagcgccatg acgttctatg
     3961 ggcaggaaaa ggtggagtat aatgcgcagc tctgccagat gaacatggcg gtgcatggac
     4021 tgaccggcgt gatccgctcc ggcgacgagg ccaacacctt ttatcacgac gcgcacggac
     4081 ttgtcggcag ctgcgactat gtgatggcca acccgccgtt caacgtggac aaggtgaaag
     4141 ccgaatcgtg cgagagcgcc gggcggctgc cgttcggcat gccgggcgtg aataaaaaca
     4201 aggaagtcgg caacgccaac tatctctgga tctcctattt ctacagttat ctgaacgggc
     4261 acggccgcgc cggattcgtt atggcgtcct ccgccaccga cagctccggc aaggatcgtg
     4321 atatccgccg gaaactggtg gagaccggcc atgtggatgt gatggtcagc gtgggaaata
     4381 acttcttcta caccaagagc ctaccttgtt ctctgtggtt ctttgataag ggaaagcccc
     4441 aagaattaca ggacaaagtg ctgttcatcg acgcccgaaa ttattatacc gtggtcgacc
     4501 gcaccctgaa tgaatggagc gattggcagc tcaaaaatct gaacgctatc gtgtggctgt
     4561 accgtggtga agttgacaaa tacgtaaaac tgctggacga ttatcggcgg tacttgctgc
     4621 attgcgcagt ggagttgaaa gccgacgatg tgtgccatgt cgtcacgcct actgccg
//
LOCUS       DAWAAE010000153         4645 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7478_length_4645_cov_3.249891, whole genome shotgun sequence.
ACCESSION   DAWAAE010000153 DAWAAE010000000
VERSION     DAWAAE010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7478_length_4645_cov_3.249891"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1140 a         1266 c         1248 g          991 t
ORIGIN      
        1 cggccattgt gcgcaattac accgcgcagg cggaggaaaa gagcgccacc gaaaccaagt
       61 atgaagccac gctcccgctc ctgcttgaag acgaggagga cgcggaataa tccggtataa
      121 tctaaaagcc acggcgtcag gcaatccaca tatgccgggc ctttcctttt cgttgcagca
      181 gaatgccagt aaaacaatac ttcttccttt tatagtaatg ggatggcaga acgactataa
      241 tgacctgctg gaaaatccgc ccatgcttca gatcacacaa tacccacacc agaaaatgct
      301 tgaaatcgcc tgagaaccca tattgtttta cacagaatgg atttgacctt agttcatatc
      361 aaattcgttt tggaggtgac cctcgcaaac cgttgagacc gaacactata tttttcacta
      421 tggcaccggt acggcggcgg agcgcgacat cgagaagatt gccgcgttgc aggagggcgt
      481 tgaagtctaa tccgaaaaaa ggtgttgtga aaatgactat atttattgtt atgctttgtt
      541 tgtctgttct atctttggcg tgctcgatct tttctttctt gcagcgaggc cctgttttat
      601 caacaatttt tttagtctca aaaaaaaatg agcaaaaaaa aatgaaaaaa agaaagaatt
      661 attatttctg cggattcatt tttgcgttat ttactattgg ctttcttcta ttaggcatta
      721 gtgccttact aagagttgct atgttaactt atgttgcggc tatattgttt gtccttatcg
      781 ctatagcaac aattgcattt tcaatttata ctgaatctaa ctgataggtg tccatcaaga
      841 tgtatctgat ggaggtatcc atgcgtcagg tagaaggacc tctccgaagc ttcgtggggc
      901 tcacccatat tggccaacga gaacgcttgt gccaacatcg aaaactggta gcccgcccct
      961 acagggtggg aagtggagaa accggagatc acatttgcat gcactgcata accatcgctt
     1021 tgtttctacg ttaaaaagca gcgagcttga agtatcaaaa cttcaagctc gctgctttag
     1081 cttccgcagg gaagtggatg atgcaatgga acgtcttttc tgccgcgtcg taatacaatg
     1141 tggaaaaacc accgtactgc tggatgacct tatttacgct tttcagtcct aagccgtggt
     1201 gctccgtgtc ttttttcgtg gttttcaagc ttccgttccg atccttcggc ggctcggaat
     1261 cgcacgagtt gacgatagag agggtcacgc ccccgaccgg gtttttcgct acggaaagct
     1321 cgatggatct tccggcagaa aggcacgcgg cctcgaatgc gttggataaa agattcccaa
     1381 acagcgccgt tatactgttg ggatccaaaa acgaaacaca atcggcgcgt acatcgaagt
     1441 gatacgcaat gccgttttgt cggcattgat ccgcgtacca aacgaggatc gcgttcagca
     1501 ccgaattatc acacagacga atcagattgg aaagctcggg cttcgcttca aagcggtaaa
     1561 ggtaatcgat aagttcctcg tttttgtttt ctctggcaag tccctctatg acgcgaaact
     1621 gatccttaat atcatggatc agcaaccgct gattatcata ctgcacctga agcatgtgat
     1681 agtaatttac atcggcttcc tctttttgaa gccgcagctg cagcgccgtg cgatcgtcgt
     1741 agattttctg gatctgatca tttaccgcca gaacaacgat attcacaaac agaaggagga
     1801 atgcgctgac catcatcagg gcttccgtgc agccggtaag cggcacggtt accctcacat
     1861 acatgatcat gatggagatc accatcgagg cggcaggcaa aaggcagatc agtaaaacga
     1921 aatacggctc ctccttcgct tttctgcgag agccataaat cctcgaaaat gccagaataa
     1981 cggaaaaata gagtatttta cttaaaacac tggtaacgat ctgcgctatc agattatagg
     2041 taaatcctca aacaaatacc atcccctttc tgtagagtaa ctataactat accataaatc
     2101 tcgaaacttt aaaggcagtt ttggcataaa tcgcgttttc attcttcggc gtgatcggca
     2161 aacagagaca gggcggccat ataaaacagc aaaaaccctc ctgccgcaac agggcttgca
     2221 gcaagagggg tttttgcgct ttgccggaaa cactccgggg tcagtcctta tacgcgatgc
     2281 actgatcaaa gagcgccgtg gcggtctcgc ggctctccgc gctcgagcac ttcgaggcga
     2341 tgaccgcggc gagaagtccg gcggcgaagc cggggacgat ctcgtaaacg ccggtgctct
     2401 tgaagagcac gagccacagc acatctacca ccgcgcccgc gacgataccc gccgccgcgc
     2461 cggagaacgt gaagccgcgc cagaagaggc tcagcacgat gaccggcccg aacgacgcgc
     2521 cgaagatgcc ccacgcgttc tcgacaagcg acatgatcgt gccgctgccg gggttggagg
     2581 cgatgagcag cgccgccgcg gcgatcacga gcaccacgat acgcccgacc cagagcattt
     2641 ccttatccgt gctctgtccc ttgcggatca cgggcttata cacgtcgctc gcaaacgcgc
     2701 tcgaagcggc gagaagctgc gaatcggcgg tactcatcgc gccggcgagg atcgcgctga
     2761 gcagcaggcc cgaaatgagt cccagccacg ggccggtaaa gatgcggcgc acgatggcga
     2821 tgaagatggt ggagctctcc tgcccgtcgc cgaggaagag gtgcgccacg accgacacga
     2881 gcaccgcaaa catgagaatg aagaacgtcc agccgatgcc gaccttcgcg gcctttttga
     2941 gttccttgtc cgatttcacg gacatgaacc ggatgatgat gtgcggcata ccggcgtagc
     3001 cgagacccca gccgagaccg gagagaatgt cctgccacga ggtcatcatg ttccagtagc
     3061 ccgcgggcag cgttccggcg gtcgtgccgc ttttctgcat catgcacagc gcgacgatcg
     3121 gcgcggcgaa aagcgagccg agcatcagca gcccctggaa aaagtccgtc cagcacacgg
     3181 cgttgaagcc gccgaggaat gtatatccca cgataatgac cgccgcggcg tacattgcga
     3241 tctgcgccgg gacgccgagc accgtttcaa agagcgtgcc gcacgccttg atgctgctcg
     3301 cggaataaac ggtatacgcc acgaggaaga tcaccgcgca gatgatctgc agcgcgcggg
     3361 agctcgcctt gaaccggttc gtcaggtact gcgggatcgt gatcgagtcg ccgcacacga
     3421 tggagaacga gcgcagccgc ggcgcaatga agatccagct gagcgaatag ccgatgaaca
     3481 ggcccaccgg gatccacgcc ttgcccatgc cgtaagcata gatggacgcc ggcatgccca
     3541 tgagcaccca tgcgctcatg tcgcttgcgc ccgccgaaag gccggacacc cacgcgccca
     3601 tttcgcgtcc gccgaggaag tagtccttct ctccggcgtt cttgctgcgc acaaaaaacc
     3661 agatgccgat acccagcata aataccagat aaaggatgaa catcaaaagc tccgataaat
     3721 tcatgtttct tcccccttga caatctacgc attacgtttt ataatgcaat gcattaccga
     3781 tgaaaatgtt atgggcgtac tatacacctt aacgcgctaa attgcaagag gaaattttca
     3841 tggatacgaa aaaatatgaa gtttttgaaa aaacggtgga gctctcctcg ctgacgaagg
     3901 cggcggagga gctggggctg acgcagtcgg gcgtgagcca catcatcgcc gcgctcgagg
     3961 aggaattcgg ctttccgctc ctgacgcgca gccgcacggg cgcgcgcctg acaccggagg
     4021 gagaaaagat catgccgttt ctgcgggaca ttctccgctc acaggagcag ctcgaccaga
     4081 cggcggcgga gctgcgcggc ctttcggcgg gcacggtgcg cattgcgacg ttcacgagcg
     4141 tggcggtgca ctggctgccg ggcatgatgc aggaatttca gacgctctac ccgcacgtgg
     4201 agttcaagct cttcaacggc gactatcacg atgtagaccg ctggctgacg gacgggagcg
     4261 tggacatcgg gttcatcacg ctgccgacgg cgctgcggtg cgaatgcatt cccctgcggg
     4321 aggatcggct tctcgccata ctgccggaga accatccgct ggcaaagaag gacgtctgcc
     4381 cggtaaaaga ggttgcggcg ctgccgttca tctcgctgct cgaatcctcg gacgacgacg
     4441 cgcgacgcgc gcttgacgcc gccggggtga agccgaacgt gcgctttaca acaaaggacg
     4501 attacgccat tctcgccatg gtatcgcagg ggctcggcgt gagcatcgtg ccggagcttc
     4561 ttctgcaggg ccaccgcgac ggcatcgccg tgcgggagat gacgccgccc tcgagccgga
     4621 cgatcgcgct ggcagtaccg gccgg
//
LOCUS       DAWAAE010000154         4632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7493_length_4632_cov_2.673804, whole genome shotgun sequence.
ACCESSION   DAWAAE010000154 DAWAAE010000000
VERSION     DAWAAE010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7493_length_4632_cov_2.673804"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1107 a         1350 c         1371 g          804 t
ORIGIN      
        1 tcggcgaaca gatcgagatc tacgatgaga ttatcgccga aaaacaggcg gagcttgaca
       61 aggctctcga aaaagaaacg gcgcagaccg agcttctccg ttcccgcatc cgcgccatgg
      121 aggagaacag cacctattcc tacgcgagct ttatctttga ttcctccagc gtgaccgagc
      181 ttctttcccg catcggcgat gtcaacgaca tcatgcacta cgacaaaact ctggaagagg
      241 agtacatggc ggcgcgcgag gatgttgagt ccatcaagaa gagctacgag gaggcccgtc
      301 acgagcagga gctcctccag aaggaactgg acaccaagca ggccgagctg gatgcgcagg
      361 tagaagcggc atacaccatg atcgccgata tcgagaccct ttccgacgac gcgcaggcgg
      421 aatacgacgc catcgccgaa gaggaagcca aggcggagga gacgctgcag gaagccatcc
      481 gcaagcgcgc cgcggaacaa gcgaagtaca accagaataa taacagcaat aatggcggcg
      541 gcggaaactc cggcggcagc ggcggcaatt ccggcggagg cggaagctcc ggcggcggtt
      601 cggcgacgag cctttccaat cttcagtggc cggtgccgag ctgcacgctc attacctccc
      661 gcttcggcta ccgcgttgcg ccgacgaccg gcgcgagcac ataccacggc ggtctggata
      721 tcggcgcggg catgggcgcg tccattgtgg cggccggcgc gggcgacgtt atttacgccg
      781 gcgcgaacgg cggctacggc aactgcgtca tgatcgacca cggcaacggc gtcgtcacgg
      841 tgtatgcgca tatgagctcc atcggcgtga gctacgggca gtatgtcacc gccggacagt
      901 atgtcggcgc ggtcggctcg accggtgttt ccaccggccc gcactgccac ttcgagatcc
      961 gcatcaacgg cgcacagacc gatccggcgg cgtacttctc cggactttcc tattggaact
     1021 gctgattttc ccttacggga tcaaaggagc ctatgaaacg taacaaatta cagaaaaccg
     1081 ttttttccgt gatttgcatc gtgctggcgg tcatcatgat gctttcactc gtcatcaccg
     1141 tacttccggc gcacgccgtg gacgagaccg atctcgagcg gctggcggaa aaacgtgcgc
     1201 agcttgaaga gcagctcatg gaacaggagc aggtcataca gggacttacg gagaaccaga
     1261 cgctgatcgt gttccgcaaa gcggcgcttg ataagcagat ctcgctcaac cgggagacga
     1321 tcgcactcat tgaagagcag atcgccgttt atcagaaact cattgaccgc aaggaagagg
     1381 agctctccgc ggcgaccgcc gcggagaccg agcagacggc gctgctgcgc gaacgcatcc
     1441 gctccatgga ggaaaacgac agctactcgt atctcgcctt tatttttgag gcgaacagcg
     1501 tgccggagat cctctcgcgc atcgcggacg taaacgatat catgcactat gaccgcgagc
     1561 tcgaaaagtc cttgcagcag agccgcgaga acgtggagac gatcaagcgc gaatatgagg
     1621 aagccaaggc cgagcaggac gccgtgaacg aagagctcat cgcccgccgc gaccagctcg
     1681 gcgcgcaggt caccgcggcc tgtgaactca tcgccaacct tgacgagctt tcgcagaatg
     1741 cgcaggcgga atacgacgcc atcgcgctgg ccgaggacga ggcgatgcag gagcttttga
     1801 aggccatgca ggaatacgcc gcgcagcagg cggcgcagag aacatcgtac agcggctcga
     1861 ccggcggcgg aagcacgccc tccggaacgg catcctccgg cgcgggtacg tccggcaccg
     1921 gcggagcata tgggaatact tccaacgcct ccggcgccta caacggtacc tttatctggc
     1981 cggtggacag cacgtacatc acctcgcgct acggcgagcg ctccgcgcct actgcaggag
     2041 cttccacaaa ccacaaggcg atcgatatcg gcgcaaagtc cggcgatccg atctatgccg
     2101 ctgccgacgg gcaggtcgct tccgcgactt acaacaacgg tctcggctac tatgtcagca
     2161 tcgagcacga cggggagacc gcgacgcggt attcccatat gacgaacttc atcgtgcagc
     2221 cgggcgagca tgtcaagcag ggccagatca tcggctatgt cggcgagagc ggcatcgcca
     2281 ccggcagcca cttggactat gccgtcatca aaaacgggca gcaggtcgat cccatgcagt
     2341 attacgacga aagcgccgtt acgtatgatc cgacggcctg accgcggcgc cgaaagaaaa
     2401 aacatccgag gtatcagcct atgaaacgcg taccgaaaat cgtctggtca ctgcttgcgt
     2461 gtgtgatatg catcgttgtc accgctgctg cgtgcctgat ccttgcggac agcggcgcgt
     2521 cctccgataa atatatggag gtcatccgcg tgatcaagac aacgttctac cagccgaagg
     2581 atacgtcctc gctggaggac gcctccgcga gcgccatggt ggcctcgctg ggcgacccgg
     2641 caagctacta tctgcctgct tccgagtatg aggagtataa actcacgctg acgaaccagt
     2701 atgtcggcat cggcgtgacc acgcagtaca acgaaaatta cggttatctg accgtccttt
     2761 ccgtaacgcc cggctctccg gccgatcagg cgcatgtgaa ggtcggcaac atgatcgcca
     2821 gtgtggacag tattgacgta tccgcctaca ctcccgaaca gtttgaccag ctgctgcgca
     2881 gctacgacgc gaaggagaca gaggcggaga agtatttcac gctccacctt ctcaacacac
     2941 aggggggcaa ggcggacgcg aaaatgaagt gcgagctcat ctatgccgag ccggtcgtct
     3001 attacattct ggacagcgac gccaacaagg cgaaccgcag ctcctccgtc ggctatctcc
     3061 gcatccgtaa ctttgacgac agcgccgccg ccagcgtaaa aacggcggtc gccgcgctgc
     3121 aggattccgg agcaacgagc cttatcatcg acgtgcgcga caatacgagc ggcaaacccg
     3181 cggaaatggc ggatgcgctc gattatttcc tgccgaaggg cgatctgttc ctcctgcgtg
     3241 accgcgacgg gaaggagacg acctattcgt ccggcagctc gtatctcaat atgccgatgg
     3301 tcgttcttgt caacgagaac accacctgcg cggcggaagt gtttgcctgc atcatgcagc
     3361 aggcgggcgt gacgatcgtc ggccgccgga cgcccggcag cgcgcagagc caggtgatcg
     3421 tggagctctc cgatggcagc gccgtccgcc tctccaaatt tgagtatctc acgccggata
     3481 aaaagagcat gaccgacctt ggcggcgtca cgccggatat cgcgtcctac cagatcgagg
     3541 acagcgatct ggacgtgcag ctcgaggcgg caaaggacgc cctgcattaa cggcgtccgc
     3601 ccgcgaaaat tctgcggaaa tctacaaaga agggaccaaa gttccgtctt gacatgggac
     3661 gccggattcc gtataatagc aggacaaatt cccgcacgga gttattctgt gcgggatttg
     3721 cgagcatgta agtatcgaaa ggaagtttaa actatgtccg ttgaaagccg ttacagaatg
     3781 agccaggagc gtctggaaga gctgaaaaaa gagctggaat atcttcagac ggttcgggaa
     3841 aaagaagtct ccgagcagat caaggaggct cgttctttcg gtgatttgtc ggagaacagc
     3901 gaatacgatg aagcgaagac cgagcagggc aagctctatt ccaagatcgc cgagatcaag
     3961 aacctgatcg aaaacgccgt cattctcgaa aacgtgccga ccggcagcgc gatctccatg
     4021 ggcagccgcg tgaccgtccg cgaagtgggc gaggaggacg aagagaccta tgagatcgtc
     4081 ggcagccagg aagccaaccc cattctcggc cgtatttcgg acgaatcccc ccttggccgc
     4141 agcctgatgg gcaagaaaaa aggcgaccgg atcgagtttg aagcccccgg cggcattatc
     4201 tactacgaga tcctcaactg cgaaaactga acgtaaatta cagatatagg agattactat
     4261 ggcagaggaa acaaggaccg gagcgccgga gctctctctc aatgaactgc tccgcatccg
     4321 ccgcgaaaag ctgacgaacc tgcaggacgc aggacgggat cccttcgcga tcacccgcta
     4381 cgatgttacc caccacagcg cggagatcct tgccgccggt gacgagctgg tcggcaaaga
     4441 ggtgtccgtg gccgggcgca tgatggtcaa acgcatcatg ggcaaggcgt ccttctgcaa
     4501 aattcaggat aagcagggcg tcattcagtg ctatgtcgcc cgcgacgatc ttggggaaga
     4561 agcgtatgcc gaattcaaga aaatggacat cggcgatatc atcggcgtca agggctttgt
     4621 gttccgcaca aa
//
LOCUS       DAWAAE010000155         4480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7766_length_4480_cov_3.852655, whole genome shotgun sequence.
ACCESSION   DAWAAE010000155 DAWAAE010000000
VERSION     DAWAAE010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7766_length_4480_cov_3.852655"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          767 a         1357 c         1313 g         1043 t
ORIGIN      
        1 cgccgacacc ggcgacgata ccgtcaagga tcaatccgct gagccattcc gccgcgccgg
       61 ccgcctcgag agcgttgccg atcagcacgg ggataccggg gacccagaca ccgtagtccg
      121 ccggatcggg ctcgtcgccg atctcgtcga gcgtggcgag cgccttttcg taatccttga
      181 gcgtcgcagt ctcttcggtg atctcgaggg tctcttcgtc ttcaacttcg taagtaaatt
      241 tgccttcggg ggcggcgccg ttttcttcaa cgtaagcgtc gtagccgtcc acgatggcgg
      301 aggcgtcgcc gtaccgctca gcgacttcgt tgtacgccgc ggagccgatg ccgaacaggt
      361 gccagccgtc gccgaacaga ccgtcgttgg cccagtcggt cgcgggcgcg ccgacggcga
      421 ccatggcgat ccagtaaacg aggaacatga cggccgcgaa gatgggcaga cccagccagc
      481 ggttggtgac gatcttgtcg atcttgtcag aggtggtcag ctgaccggcg tttttcttct
      541 tgtagcaggc cttgatgacc tcggcgatat aaacgtagcg ctcgttggtg atgatgctct
      601 cggcgtcgtc gtcgagctcc ttctcggcgg cctcgatgtc ctgctcgatg tgggccttga
      661 catcctcggg gatgttcagg gactcaagca ccttatcgtc gcgctcgaac accttgatgg
      721 cgtaccagtg ctgctgctct tcgggcaggg tgtgcacggc agcctcttcg atgtgggcaa
      781 tggcgtgctc gaccgggccg gagaacttgt gcatcgggat ggtcttgccg ctcttcgcgg
      841 ctttgatggc ggtctcggcg gcttccttga cgccgtcgcc cttcagcgcg gagacctcca
      901 caaccgggca gccgagctga cgggacagct ccgcaacgtc gatcttgtcg ccgttcttgc
      961 ggacgacgtc catcatgttc accgccagca ccacggggat gccaagctcg gtgagctgcg
     1021 tggtgagata aaggttacgc tcaaggttcg tgccgtcgat gatgttcaaa atggcatcgg
     1081 ggcgcgtgcc gatgaggtaa tttctcgcta cgacttcctc cagcgtgtag ggggacagag
     1141 aataaatgcc cggaaggtcg gtgatgataa caccgtcatg acctttgagc ttgccttcct
     1201 tcttctcgac cgtgacgccc ggccagttgc cgacgaactg gttggagccg gtcagcgcgt
     1261 tgaacagcgt ggttttgccg ctgttcgggt tgcccgcaag ggcgatgcgc aaatccattt
     1321 tcacgcttcc tttctgctct caaaagcttt tccgcggttt gccttcgcta accgctgctc
     1381 gaaaaaatta ggggctctcc ccgaaaagtt tttattcgat ctcgatcatc tcggcgtccg
     1441 ccttgcggat cgaaagctcg tagttgcgga cggtgacttc gatggggtcg ccgaggggcg
     1501 ctaccttgcg gacgtagacc tgggtgcctc tcgtgatgcc catgtccatg atgcggcgct
     1561 tcaccgcgcc ctcgccatgg agcttcacga ccttgaccgt cgagccgacc ggcacttcgc
     1621 gcagagtttt catgccattg cctccttatt ttatattttc tttttctttc ctgagcgttg
     1681 attcgcaagg gcgcgcgccc ttgtccatca acgcatgatc aaaccatgat cttgcgcgcc
     1741 agctcatcgc tgacagcgac gcgggactcc ttgaccttca cgatcacgtt tccgcccagc
     1801 gtggtcacga tgcagacctc gccgccgacg gtgaagccaa gatcctcaag atgacgcttc
     1861 gtctccgggt ttccgccgat gcgctttacg atctgcggtt cgccgacatt cgccagtacc
     1921 aacggcatca tgccgcatcc tccttccgga tgttagccca agctaacata tgcgcaaaaa
     1981 taatcggtta gctctcacta accgccttat ataatagtta ccccgcgcta acttgtcaag
     2041 ggggaacgcc gctttttttt atctttgtat aatcggcaca aaaggacagg ctccgacacg
     2101 gcatgccgtt ggaaattcgc ttgtttttcg gaaatgaata tgatatggta atagataatc
     2161 atccaaggaa acggagtttt tttctcatgg tcatgcagga atccggggaa atgtacctcg
     2221 aaaccatata tcggctgagc cagtcgctgc cggtcgtgcg ttcgatcgac gtgagcgaat
     2281 acatcggcta ctcaaagccc tcggtcagcc gggcgatcgg cattttgaag aagcaggaat
     2341 acatcacggt ggacaagttt ggcgcgatca cgctcacgga caagggccgc gagcgcgcgc
     2401 agtatatctt tgagcggcac aggtgtctca cgaagatatt caccctgctc ggcgtgaacg
     2461 cggagaccgc cgctgaggac gcctgccgcg tggagcacta catcagcgac gagacgttcc
     2521 aggccctcaa acggcatctc gcagagcacg aaaactaaac gccctgaccg caaaaacccc
     2581 tccgtcaaaa gcaagtcctt tgacggaggg gttttgtatc agtatgtctc aacgatccgc
     2641 acgcggtttt cgccgtcggc gagaatttcg gagcatggac ttattggaag aggatcattc
     2701 gtcctcttcc gttttcgttt cgccttccgc ttcggcgggc gcttccgtct ccgtcgcggc
     2761 cttttccggc tcggtctccg gaacgtcctc cgtttcggtc ttttcgacgg tttcggtctt
     2821 ttcggtctcg tccgtcgtca cttccgctgt ctcggaagtt tccgtctctt cggtctcggc
     2881 gggcttcttt ctgccgagca gcaccgccgc cagcgccacg atcacgacga gcgccgcgat
     2941 ggcaacgtac atccagacgt tcgccgactt cgccgccggt tcctccggaa cggtgaacag
     3001 ctcgttataa tccatcgtgc cgaaatcgcc cttccactcg acctccgcgt tgttgatcca
     3061 gtcgatgagc atgttgttga aaagctcgtt ggcggcgtcg gcacgcagcg tcatataatc
     3121 gcccgtgccg gaatcctgcg agagggtctt gccgtcggga tcgagcggga gacggaggat
     3181 gatgtggtag ccgtagctcg actccacgat gtcggagatc tcatagtccg cgagagcgcg
     3241 ggcgccgtcc tcaaactcct gcaccatctg atcgtcggtg aaaatgtagc cgtccgggaa
     3301 gtttgtcatg ccggggtcgt cggcttccgc catccgctcg tcgaaccgcg ccatcatcgt
     3361 ctcgcggtcg gattcgagcg cgcgcagctc ctcgagcgtc gtctcggcct ccttgcggac
     3421 ttcggctttc tcttcgtccg tcatgtcggt gccgtcgtcg ttcttcgtga ggaagagaat
     3481 atgcttgcag cggacatagt tgttgtcgtt cgcccacgcg aggatgtccg cctcagagag
     3541 cttctcgccg ttcgcgccgt aggtatcctc catgatggcg ttgaacgcgg cgttgctccg
     3601 gaggagcagg cggtaggtcg gctcgtcgag atacgcgctc tccatggcct ccatcagcgc
     3661 gtccacgccg ccgtactgct ccacgatgct gttccactgc gtttcgacct cggcgtccac
     3721 cgtctccgcg agaccgcgct tgtccgcctc ggccgccgga acggtgtaat acttcatgcg
     3781 cgcctcaagc gcttcgcgca tcgcgtcctg cagcgtcgtg ccgtcgcccg tatcggtggt
     3841 aaagtcgggg atcgccatgg tgtagcggat gaactggctc gtcatgctgg cgatgagata
     3901 gtagcagagc ttccatgtga cttccgtgcc gttcaccgtg gcgacgacgg tgtcgccgtc
     3961 gtactttttg ctggccgcgt cgagctgttc catgatcttc gtatatgctt cctgcgctgc
     4021 cgcggcagcg tcagatgcgg cttcggatgc ggcgtcggat gcagcgtcag atgcggcttc
     4081 ggatgcggct tcggacgagc cggccgcggc gctgtcgccg gtcgtgccgt cgtcggcggc
     4141 gagcgccgga acggcgagcg cgccgagcag cagcacaagg ctcataagca gcgcgagcag
     4201 tttctttgct ttcatagttt ttttactcct tttccgcggc gggctccgcc gccggttttt
     4261 gcagtttgtt ccacgcttcc acaagggcgc gcgcttccgg aatgggtctc gcgccgggcc
     4321 ggagcttaag ccccagccgc ggctcgcgtc cggcttcgag cttgacgcgg tttttgaact
     4381 ccttctgcgc atagagcgcg gaaagcgttt ccatgtcgaa attctctacc ttgaaataga
     4441 gcatgtttcc cttctgggag atgtccgaga taccggcgcg
//
LOCUS       DAWAAE010000156         4479 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7771_length_4479_cov_3.106465, whole genome shotgun sequence.
ACCESSION   DAWAAE010000156 DAWAAE010000000
VERSION     DAWAAE010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7771_length_4479_cov_3.106465"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          882 a         1347 c         1265 g          985 t
ORIGIN      
        1 gttccacccg aaacaagatc ttacttcgta ttcccgcgct ttttgagata caccgcgagg
       61 atgatgccgg cgatgagcat cacgacgccg atcacgatga tgaccgtgcc gaggatggcg
      121 acggggttcg taaagccgct gctgcccatg ccggacgagc cgaacgggga ggagatgacg
      181 ttcccggcgc tgtcatacca gatcgtgccg cttcccgcaa agtcggagaa gccgccgtat
      241 ccgccgaagg gatccatgcc ggtgttgccg aagaacgaac gctccatgct gagactgacg
      301 ccgcgcgcga ccgcgccgat cacgatgaag ccgagtcccg cgagcgcggt atacacgccg
      361 tagagccagc catagcgccg gatgagccgc ccgatcacgc cgggaatgga ttcgacccag
      421 ttggagctct gtgccggggc ctccggtgcg gccgggattt ccgattcagg aaatgtctcc
      481 ggttccggct cgctctccga gagcagccag tctgtcgtca cgtcaaacgc tctggcgagc
      541 aggacgagct tcgagagctc cggcacggac tcgccggtct cccatttact gatcgcctgc
      601 cgcgacacgc cgagcttttc cgcgagcgcc tcctgcgaaa ggcccgcctt tttccggcag
      661 tacagaattt tttccgatag attcataaag gtgtgtcctc cttgaattta tggcatcagt
      721 atgacatgga ggccggttgc gaagccaccg ttccggcttg gaactttcgc aaccggcggt
      781 tgcagaagct attataacag aaaaacaccg caaaagtaca ccagaaaaac acaaatgttt
      841 caccgaaaaa gacttgctta tgcgtcggaa aagatgtaca attaatttca tattttaaat
      901 tgcaaaataa ttttgcaaca tgttatgagg gaagagatat gaaagagaga ttgaatttcc
      961 ggcagctcat cgccgtggcg agcatgctgt tcgggttgtt tttcggcgcc gggaacctga
     1021 tattcccggt gcatatgggt cagctcgcgg gcgcgaactc ctatattgcg gcgctcggct
     1081 tcatcgttac ggcggtaggt ctgcctctta tgggcgtggc ggcgttcggc atcagccgtt
     1141 cggacgatct gctcgccatg agcagccgcg tagggaaggg gtacggcgta ttctttacct
     1201 gcgcgctgta tctgacgatc ggcccgcttt ttgcaatccc ccgctgcgcc gcgacgagct
     1261 ttaccatcgg cgtggagccg atgctcaccg gcggtgcggt aacggagctt gccatattct
     1321 ccgtgatctt cttcgcgctc gtgctgttct tctcgctgcg cccgagcaag atcctcacat
     1381 gggtcgggcg gatcatcaac ccgctgttcc ttgtgttcct tgctgtgctc gttgtcacgg
     1441 cgctcgtaaa gcctgtcgct gccgtttcgt cggtcgcgcc ggagggcgca tacgcctcca
     1501 gcgccttctc cgccggtttt ctggacggct acaatacgat ggacgcgctt gcctcgctgg
     1561 cgttcggcgt gatcgttgtg ggcgtcatcc gcgatctggg cattaagcac gacgccgccg
     1621 tcgcgggcaa caccgtgcgc gcgggcgtgt tctcgtgcgt gtttatgggg ctcatctatc
     1681 ttgccatgac gatcatcggc gcgcagagcc gcggcgcgtt cgctgtgagc gccaacggcg
     1741 gcatcgcgct tgcggatatc gccggatact atttcggcac cgccggacag gtcatcctcg
     1801 ccgttacggt gacgctcgcc tgtctcaaga cggccatcgg ccttgtcacg agctgcgcgg
     1861 agattttccg cgtgctgttc ccgaagtcca tgagctataa ggcgtgggcg atcgtgttca
     1921 gcgtcgtgtc gctgctgatc accaacgtcg gcctgacgca gattatcgac tattccgtgc
     1981 cggtgctcat gttcctctac ccgctcacca tcacgctcat gctgctgagc ctgttcggac
     2041 atttctttga ctacgacaag cgcgtgttca tttcggtcac ggcgttcacg ctggccgcgg
     2101 cggtgctgga tctgctcgcc gcgctgccgg tcggagcgcg tgccttcctg catgtgaacg
     2161 gctttgtcgc cttctgcaag gagagcctgc cgcttttcgg catcggtctc ttctggctca
     2221 ttcctgccgc cgtcggcctt gtcctcggct tcattctccg cgccgccggg aagaaagcga
     2281 cagcgtgata agcgttttca tctgcgccgc ttacagcccc ctctgacgag agagctgtcg
     2341 agcgaaacga gacggaggga gagaaaaacg gcgttttccg cgtcgaacgt ctttctctcc
     2401 ctccgtcaaa atatcaattt tgccgcctcc ctcgtcagag ggaggcgttt ttttattcct
     2461 ccgcggcttt ggcgttcttt atataccggt agacgctcgc ctgcgagcag ccgagctttt
     2521 ccacggtgaa ctgcactgcg ccgcgcagct cgaacatgcc ctgctcatag agccgcgcga
     2581 cgatctcggc gcgctcggcc tgcgtcagac ggcgcagcgg cacgccggcg tccctcgtca
     2641 cggtcgcgaa caggtcctcc atcagcccgt tcatgtcgga ctggaaatgc tccgccggct
     2701 gcgcggcggc atgctgcggc gcggcgggct gcttggcggg cgcgtcgtct gggaagtagt
     2761 ggcggtggac aaagtcgtcc gggtgcacca gccggaggat ggtgtcgctg acctcatgga
     2821 agcggctgtc gtcaaagttg acgcacaaaa gccccacgag ctttccgccg tccttgatga
     2881 acatcgtgct cgaacggagc gtttttccgt tggataactg gcccgtgtaa ttaataatat
     2941 agttgttgga ttcatattgc ttctgcatga tcatgcgcag cgccgcgttg gtgatcggcg
     3001 cgcctacgtc ccgtccgctg atgccgccgt tggcgatcgc ggcaacgcgg ctctgctccg
     3061 gcaaaatttc atgcagaatg atctcataat ccggcccgag aacgtggccg agaaactcaa
     3121 caagccttgt atagtgcaaa agcagttgtt ccgtcatggg ttttgtacct cccttggagt
     3181 gcggtgattc tcatactacc ataccaaaat gcccgagaca agaaaaaaac cgcgattttt
     3241 cgggaatttt ctcacaacga gaagaaaatt tctcgttata taagtttttt tcactaaaat
     3301 ttgtgagcaa taaacaaaaa tatatttaga ccttgaaaag atttgtgatt tatattgaca
     3361 gcgttttgcg gcgatgataa actattctca caccaaggag gtgaaaacca gaattgaaaa
     3421 acgttatcgc aaccgaaaag gcccccgcgg cgatcggccc gtattcgcag ggcatccggg
     3481 cgggcagcct gatcatcacg tccggccagc ttcccattga tatggccacc ggtgcgttcc
     3541 ccgccacgat cgaggagcag acccgcaagt ctcttgaaaa ctgcaaggcc gtcctcgagg
     3601 cttccggcgc aaccatggaa aacgtcatca aaaccaccgt tttcctgagc gacatgaaca
     3661 acttcgctgc catgaacggc gtttacgcca cgttctttgc cggggactat ccggcgcgca
     3721 gcgcggtcga agtcgcccgc ctccccaagg acgcgctcgt tgagatcgag tgcatcgccg
     3781 tcctttaatt tctttcgtcg atctgatact gtccgccagg cgccggcagc gagctgtctg
     3841 cgacgaccgg ggtatctcca aaacaaaagg cgcaaagcgc caacattatg gaggtcagta
     3901 ttatgagtaa agagtatccc ctgaacgttc ccgcgcccca tcatttcacg ttcgccgtcc
     3961 gcgacattcc cgatgtcacg gtcgaacagc gtgagcgcgt cctgaaggcg acgcattaca
     4021 acgagtttgc cttccccgcc ggcatgctga cggttgatat gctctccgac tccggcacca
     4081 ccgcgatgac caaccagcag tgggcgtccc tgttcctcgg cgacgaggcc tacggccgca
     4141 acaccggcta ctacgtcctt ctcgacacct tccgcgacat cttcgagcgc ggcggcgaga
     4201 agaactggaa gaagatcatc gactatgtcc gcaccgactg ccgcgacgtg gagaccatga
     4261 tggagaacgt ctaccttccc gagtacgaag gcggactgtt caacggcggc gccgcccaga
     4321 tggagcgccc caacaccttc atcatccagc agggccgcgc tgcggagtcc gtcctgatgg
     4381 agatcgtcaa gaagatcctt gcccagcgtc atccgggcaa ggtgttcacg atcccctcca
     4441 acggccactt cgacactacc gagggcaaca tcaagcaga
//
LOCUS       DAWAAE010000157         4406 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7920_length_4406_cov_2.611354, whole genome shotgun sequence.
ACCESSION   DAWAAE010000157 DAWAAE010000000
VERSION     DAWAAE010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_4406_cov_2.611354"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          804 a         1421 c         1342 g          839 t
ORIGIN      
        1 ttctcccaga ggacgagatt cacgctctgc ccgagagcgc ttcggtattc ccgctcggta
       61 tattctgccg cgggtgccgg gaagccgaag gggtagagcg cgccggtcga aacgcgctgg
      121 agcgtcacgg gaagccgcgt ctctccgaat gccagacagc cctccgcata aaaggaaccg
      181 tccggaaaaa tatacccgct ttgcagctcg tcggtaccgt cttttacata cggctgcatg
      241 ccgttgcagt catacagctc gtctcgtccg gtcaaaggga agctctcgga ataaagttca
      301 agaccgtagt tctccgcgat ctcccgcagc tttgccgcgg cctccggcgt cgtcgcccgg
      361 tatgtctcac aggttttgcg cagagcgtcg tccccttgcg tgaagctcca gtcgggttcc
      421 gtccaggtct tccgctgtgc cgcggcccac gtccaccatg cggcggtgcc ttcgtatgtt
      481 tttgtgccgc gcagtccgtt cggcacaacg atgacgcggt tcgttccctc aatggaatat
      541 gtctgatcct tccagtgcag cagcccggca gcataggccg cggcagcgag cagcagcgcg
      601 agcgccgcgg caagcagcgc cgttttccag agctttgcct ttccgcgtcc gtgcggcttt
      661 tcctcgtcga tccggagcaa atcccccgct tccagaacat agcggtcgga tacgccgttc
      721 agcgcgtcaa acagaatatc tccgccgttc attcaatatc cctcctcttc cagcttttgc
      781 gccaggcgtt tccgcgtccg gtacagcgag gttttcaccg cggcggtctt catgccgagc
      841 cgcgcggcga tctcctccgc ggtcagcgcg tagaagtacc gtccgaggaa aatgacccgc
      901 tcacgtttgg gaagcaatga gaggaacgca tcgatgctcc ggagaagctc gcggttttcc
      961 gccgtccgcg ccgggtcgct ccctcccggc aagcactcgg agagctcgtc gatcgcaagg
     1021 ctcgcttccc cgccgccgcg cttttggctt ccgctgcggc gcaggcgtgt tacggaaata
     1081 ttgcgcgtga tccggccgag aaacaccttc aggctgttcg ggcgctccgg cggcatggcg
     1141 ttccaggccg cgagccacgt atcgttcacg ctctcctccg cgtcctcggc attcctcagg
     1201 agattatacg ccaccgtgtg gcaatacggc ccataggcat tctccgtttc cgggatggcg
     1261 cgctcggagc gctgccaata gagagcgatg atgcccgcgt cgttcatgct tcccacctcc
     1321 ttacactcct aaagtcgcaa aagcggagca aaaggtttca tattttctga aaaaagctgc
     1381 cgtggctttc ggtcgcggca gcttttggat ttctattccc cgctccggag cggcaggttg
     1441 gagagcgctt cggatacata gtcattgaat ttatagacgt ccttcgtgcg ggcgatcttt
     1501 ttggcgagct cgtcggcgcc gatgaagagc gaagagagat agtgccagag ctccttcatt
     1561 ttccgcatgg cgttcacgcc gccgagctcg gcgcggtacg cctcgtacag ctcctcgtgg
     1621 aactgccgca gctcctcgcg ggaggccgcc gcgccgccgc gcagacggcg gaacagcgcg
     1681 gggtcggccg ccgcgccgcg gccgagcatg accgcctccg tttccgggtg ggcggcttca
     1741 aacgtgcgga tgtcctccgc agtgaagaga tcgccgttgt atacgaccgg gaacgggctt
     1801 cccgccgcgg cgagcgcgaa ggcgtccata tgcaccgcgc cggaatacat ttcctttcgc
     1861 gtgcgcggat ggaggatgag ctcctgcgcg tcatattgcc ggtagatcga cagaaggcgc
     1921 ggaaattcct ccggtgcaag atacccgatg cggcttttga gcgacacggc aacgggcgag
     1981 gcggcaaaca cgtccgcgag cagcgtgtcg agcgcgtccg gcttttgcag aagccccgcg
     2041 cccttgccct ttgccgtcac ggttgccgac gggcagccga gattgaggtt aaagaccgta
     2101 tatcccatgt cttgcagccc ctcggcggcc caaacgatgt ccgcggcgca cttggagaga
     2161 atctgcggca cgaccggcgc ggcgcacctc atctccggcg tcagctcgcg cagctcccgc
     2221 ggcgtaaaga gatggtcgga cgtcggggag aaaaacggcg tatagtaccg gtccgcgccg
     2281 ggaaaacggc gcgaatggat cgcgcggaaa acgtgcgtcg tgatcccctc catgggggcg
     2341 aaatagtatt tcatgtctgc ataactccat aagaaagatg cgatcgtccg cgggcggagc
     2401 gaagccccgt ccctaccggt ttttcattat atcgcatagc ggcggcagtt gcaaaacatt
     2461 tgacgcgcgt gctatactta gacagcatta caggaggcat cgtatgaaca ttaaacgctt
     2521 tctttccggc gcgctgtgcg ccgtgcttct gctcgcgctc tgccccgccg cggcggcgga
     2581 cgcgccggtg gagatattct ccgcggagga gctttcgctc ctgcgcgaga cgcccgacgg
     2641 cgactttgtt ctcaccgccg acatcgacat gaccggcgtg gactggcagc cgatcgcctt
     2701 ttccggcacc ctcgacggcg gcggacatac catttacaat ctcacggtca cctcgctcgg
     2761 cgcagaccgc gccgagacgg tggacgggaa cgacaagacc tacgacaccg cgctcaccgg
     2821 ctttttctcc gtgctcaatg gcgcttcggt ccgcgatctt tcgctgcgcg gcgtaagcat
     2881 cgccgtggag acgccggaga atgtgttcgc cggaacgctc gcgggttttg cttcgcctga
     2941 aacggtgctc gaaaacgtct ccgtttttga tgcccgcatc gatctcacgg agacatgcca
     3001 gcgcgaaccc gccgatgagc acgaccgctg catcgccggt atcggcgggc tcgtcggctt
     3061 cggcggcgga acgtatacga actgctccgc ggattccaca ctcgttttct ccgatgagag
     3121 cattgcgtcg ctccgctgcg aggagtttct cggcggcatt ctctcctgcg gcaacgcggc
     3181 actcaccggc tgcaccgccg cgatcgacgg ctacgccgcc tgccgcggct atgcgcacaa
     3241 cggcggactt gtcggtatgt tctatcagta cgacaagacc gtggacatcg gcacgatctc
     3301 cggctgtgcc gtgaccggca agatcacgtt tttcgaggat aacgccgacc ggcgcgccta
     3361 ctgcgaagcg tttgccggag agtttctcac ctggacgaac atcaccgaat gcaccgccga
     3421 ttttcagcgg aatgagatct tcgattactc cgccgccgta aagccggaaa aatgcgctga
     3481 accgtcctac accgacacgg tggtcaccgg gaactgcgat accttcggct ataccgagca
     3541 cacctgtgcc gtgtgcggct attcctaccg cgacacattc acgccgccgc agcacacccc
     3601 cggcgagtgg acggaaacga aagcgcccac cgagaccgaa gacggcgagg agacgcttcg
     3661 ctgcgcgctc tgcggcgcgg aaatggaaaa acgcgccgtg ccgaagcacg tttccggcga
     3721 ctggatgacc gtcaccgcgc cgacgtatga ccgcgagggg ctgcgccagc gcttctgcgc
     3781 tgactgcggc gtgctgctcg gcgaggagag catccccatg ctcatcgccg cgagcggcat
     3841 agagggactg ccggacgcgc tcacgctgca ctataaggac accgtgacgc tcgaagccgt
     3901 gatcacgccg gaggacgctt ccgacaagac cgtccgctgg tacacgaaca tcaacattgc
     3961 ctcggtcgat ccctcgaccg gcgaggtgcg cgcctgcgcg cgcggcgaga ccgttctcac
     4021 ctgcgtttcc ggcgacgggt tcgtcacgaa ggagatcccc gtaacggtcg actatactat
     4081 cgggcaatgg ctcatcaaaa ttctcctctt cgggtgggcg tggtactgat ggaaagctac
     4141 accgtcatcc gcgcaaaccg taggaccgtc gggctgcggc tcgataaaga cgggcggctc
     4201 gtcgttcgcg cgccgtattg cacgtcggag gaagagatcc gccggatcgt ggaaaaggag
     4261 agcgcgtggc tcgcgcgcga gcatgagcgg ttcgcggcgc ggcaggccgc gcaccccgag
     4321 ccggacgaag cgcagcgccg tgcgctcatc gaaaaggcca aaacgaccct tccgccgaag
     4381 atcgagcgct atgccgcgct catggg
//
LOCUS       DAWAAE010000158         4374 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_7982_length_4374_cov_3.783283, whole genome shotgun sequence.
ACCESSION   DAWAAE010000158 DAWAAE010000000
VERSION     DAWAAE010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7982_length_4374_cov_3.783283"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          780 a         1362 c         1301 g          931 t
ORIGIN      
        1 tgctcatcgc cggtgtgctg cggctggggc ggctcatcag cttcatcccg atgcccgtca
       61 ttatgggctt tacgtccggc attgcgatca tcatcgcgct cggccagatc gacaattttt
      121 tcggcacgac gtccgagggc ctgacgaaca tagaaaagct gctctcctac gggcggctcg
      181 ggttccatgt gaactggcag gcggcgctca tcgggctgct cgtcgtggcg gtcatggtcg
      241 tctggccgaa aaagtggggc gcacgcgtcc ccggctcgct cctcggcatc atcgcggccg
      301 ccatcgtcgc gggcgtgttc catatggatt ctctcgccac cgtcggcgac attccccgca
      361 cgctgctgct cgccgaccgg ctggatcttt cgtcgctcag cttctcgatg gtcaaagacc
      421 tgctctcgcc catcgtgacg atcgcggcgc tcggcatgat cgaaagcctt ctgtgcggcg
      481 cttccgcctc acgcatgaag ggtgaggaat tcaatgccga tcaggagctg atcgcgcagg
      541 gcgtcggcaa cattctgctg ccgctcttcg gcggcgtgcc cgctacggca gcgatcgccc
      601 gcacgagcgt ggcggtcaag tccggccagc agacccggct gacgagcgtt tttcactcgg
      661 tgtttctgct cgcgtccatg ttcctgctcg gcggcgtgat ggcgcgcctg ccgctctcgg
      721 cgctcgcggg cgtgctcatg gtcacggcgt ggcgcatgaa cgactgggcg ggcatccgct
      781 atgtgttccg ccacaaattc aaatccggca tcagccagtt tctgctcacg atgatcgcaa
      841 cggtcgtgtt cgatctcacg gtcgccatcc tgctcggcgt tgtctactcc gccattttgt
      901 atatggccaa gtccagccag atccgcgtga gcttttcggc catcgatacg aacaagctcc
      961 gcgccatcga cggcaagccg ccgatcctcg aatcggcggg cgtggcgtat gtgacgggcg
     1021 cgctgttctt cggcgcggtg gacgagttca accaccgtat ggcggagatg cccgcctacg
     1081 accacgtgat cctctcgatg cgcggcatgc cgagcgtgga cgtgtccggc gcacaggcga
     1141 tcctcgagct gtgccagagc cttctcgcgc agggcaagac cgtggcctgc tgcggcatga
     1201 gcgaaaacgt ccggcggtac tttgaccgcg ccgggatcac cgaagtcctc ggcgagagcg
     1261 cgtatttctg gagcgctgac caggcgatcc tcgatctgct ggacgcggag atcgcggaat
     1321 aaacggcata aacgaatgga gccgcggagg aagttttcct ccgcggctcc gcttttttat
     1381 tcgtcctttt ccgtttcggc ggcgcggatg cggaggtaca tgtcctgcat ctggcggtcg
     1441 atgtcggcga tcttgccgga gactgcgagc atttcctcgt tgatgccctc cggcagacgg
     1501 gatttgtcgg ccttataccg gatatcgtgc tcaagactcg cccagaagtc catggcgatc
     1561 gtgcggatct ggatctcggc cacgacatac tccgtccggt cggagaggta caccggcacg
     1621 cggatatcga gatggagcga gcggtagccg ttataattcg gcgttttgat atagtcctgc
     1681 cgcttgacga tctcaatatc cttctgccgc tcgagcattt cggcaacgcg gtagacatcg
     1741 tcgatgtagc tgcacaccac gcgcacgccc gcgatgtcgt tcacgtgctc gcgcgccgtc
     1801 tcgatggaga ccggcatgcc ctttttcatc agcttcgcgg cgatgctctc cggcgttttc
     1861 acgcggcttt cgatgtggtg gatcgggttg cgcgcataga ggacgctgaa ttcgtcgttt
     1921 agaatttcaa atttcagcgt gagctggcgc gcggcggcct cgtaaagatt gcgcatcacg
     1981 agaaattcgc gcagcttgcc ctgcagctcc ggtttttttt cggcgtcgtt catggtttct
     2041 cctttgcatt ggccgcggca gcggggaccc tctccgtctt ttgccttcgg caagatccac
     2101 cttccctatt aggggaggca agtggggcat ccgaaaacta tcttggcccc cctaacaggg
     2161 gggctggcag ccgcaggctg actgaggggt tttctcactt cctgtattga gtataccaca
     2221 tcccccgcaa tacgcacaag cggcgggaac ttttttccta tgccgcgcgt ccaaagaata
     2281 gaagcactct caaaggagga aatattatga aacgccttat cgcccttttg cttgcgcttt
     2341 ctgcgctctt ctcccttgcc gcgtgcggcg gcacaaacgc ctttgctctt gccgaggcga
     2401 cctacccgga aagcgcgccg tatccgaacg aagcgaagta ctccggcgcg aagtatgaag
     2461 cggcgtacga tgcctggagc gccgaccgcc gggcacggaa caccgcggcc tcgtcggtcg
     2521 atggcgcgct cgacgattat atccgcgcgg cgctgcccgc gctgctctcg ggcgcggacg
     2581 acaaaaacgc cgtgtgctcg ccggtgaacg tgtatatggc gctctcgatg ctcgcggagg
     2641 tcacggacgg ggacacccgc gcgcagatcc tctccctgct cggtgcggac agcattgagt
     2701 ccctgcgcaa gaccgccggg aacgtctggg cggcgaacta ccagaacgac ggcgcggtga
     2761 cgagcatcct tgccgactcg ctctggctct cggacagcat ggactacaat tccaacaccc
     2821 tcgcgcgcct cgcggacagc tactatgcct ccgcctaccg cggcgagatg ggctccgagg
     2881 cgttcaacga agcgctccgc cgctggatca acgagcagac gggcggcctt ttgaaggaca
     2941 gcgccgacgg tctttcgctc gcgccggaca cggtgatcgc gctcgcctcg accatatact
     3001 tccgcgcgaa gtggggcagc gagttttcaa agagcgccac ggcggacggc gtattccacg
     3061 cgccggacgg cgacgtgacg cacaaattca tgcacgagac catggagagt acgtactacg
     3121 ccgccgataa ctttgctgcc gtcggcaaat atctcgaggg cagcggcacg atgtggttcc
     3181 tgctgccgga cgagggcgtg acgccggagg cgctgctctc cgacggcagc gcggtcgatt
     3241 tcttcctctc gccggagagc gctgagagca agtatatgac cattgatctc tctgtgccga
     3301 aatttgacgt ttccgctgat accgaccttt ccggcgcgct gcgcgcgctc ggcgtgacgg
     3361 acgtgtttga cgtgggcaag gccgacttct ccccgctcac cgacaaggcc gacagcattt
     3421 tcctttcaca ggccaagcac tccgcgcgcg tcaccattga cgaggagggc gttgtagcgg
     3481 cggcctacac cgtgatggtc gcgtgcggag ccgccttgcc gccggatgag cgtgtcgagt
     3541 tcacgctcga ccggccgttc ctcttcgccg tcaccggcga gagcggcgag atcctcttcg
     3601 cggggatcgt gaacacgccg gcatgacagg gcagaggccc attttaaaat tataaacgta
     3661 tgcccgcccg tgaggacaac tcacgggcgg gcatattttc gtttccgctc aaataatata
     3721 atcgtatccg ccgggatcgt tgtgcatcag atcggcgagc gagaacttat ccagataatc
     3781 gttgatgact ttgtccagcc ccttccacag ggaaagcgtg cggcactccg ccgcccgggg
     3841 gcacgccgcc gcgccgggct ccagacagga gacgggcgcc agcgaatcct ccgtcatacg
     3901 cagaatgtcc ccaaccgtat attgctccgg cgcttttgtc aggcggtatc cgccgccctt
     3961 tccccgcacg ccggtcagca gctttgcttt cacgagcagc ttgatgatgt tctccagata
     4021 cttttcggag atattctgcc gcgccgcgat catcttcaga ggaatgaatc cgtccgtcga
     4081 atgctcggcc agatcgacca tgacccgaag cgcgtaccgc cctttggttg agatcagcat
     4141 tttccgttcc tcccttgtgt ataaatccta ttatatagca gggaagtagg taaatcaaca
     4201 aataatcttc cttttgctat tgacaaatgc gcagcggcga tttataatcc gcacaaacct
     4261 acaagaaagg ttggtattga aatgcgcttt tccatggccc tccgatgttg ccgggcacct
     4321 agcgtcgatt gacaagggca cacgcggttc tgccgggcgg aaatgccctc atac
//
LOCUS       DAWAAE010000159         4263 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8211_length_4263_cov_2.633555, whole genome shotgun sequence.
ACCESSION   DAWAAE010000159 DAWAAE010000000
VERSION     DAWAAE010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8211_length_4263_cov_2.633555"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          928 a         1196 c         1288 g          851 t
ORIGIN      
        1 attcatggtc ggagaggacg cacacggcgt cggcgcggcg catcacgccg taagggttgt
       61 cgagcgggcc ggtcagcgtg aaatagtctt caagccccgc atcccgcacc gcaagccgga
      121 gcttttccac gagcagggcg ttggcaagcg agccgatgtt cacccagcga aaccgcagcc
      181 cctcggaaaa gagccgctgc atcgccgcga cctgccgcag atggtttttc tcctcacgca
      241 cgtttgcgac cgtcacgagc agcggcagac cgtcctccgg cagagagacg ggcagcggtt
      301 catcactcat ggcgatgatc ttctcgctgt ccacacggtt cggaaggaca gctgagcggg
      361 aggcgagctg cggatatttt ttcaccgccc cctccatcga gtggcgggag gcgaagagaa
      421 agccgtcgaa ctgcgcttca aagcgcagga tgtccgggtg gagaaaggcg gccttgtcca
      481 tgtctgcgtg gatccagaca tatttgcgcc gtgccgcggc atagcgcgcg cagagcgcgc
      541 tcgaaaacca ctcgccgtag gaaatggcgg cgtcgtagta ttggttttgc agatagcgga
      601 tggcgccgcg gcggaacggc tgcgtgccgg tgagcttccg ctcaagcttg aagacggcct
      661 ttttcacgaa cgcggtcttt ttttcgtttt cggccacgtt gatgacgtgt acccagtccg
      721 gtatctccgg tatgagggag agactgcccg gcaggtcgat catatcaaag atgagaagat
      781 caacgtcaaa gcgcgcgctg tccatcgtgc gcagaaaatt cacgagcgcc gtttccgcgc
      841 cgcccctgaa gaactggttg atgataaaaa ggacgcgctt tttcttcggt gcggcggtac
      901 tcatgcgcgc atatactccc cgcacacggc gtccgcatcg ccgagcatgc gcatatggcc
      961 gtgctcgagc cagagagcct ttttgcagat cttctgcacc tgctcgatcg agtgggagac
     1021 gaaaacgacc gttgcgccgg aggcgatcat ctcctgcatg cgcgcgaagc tcttctcctg
     1081 aaatttatag tcgccgacgg cgaggatctt gtcgatgatg agaatgtccg gcacgttcat
     1141 cgtcgcaatg gcaaagccga gacgcgccac catgccggag gaatagttct tcacggcggt
     1201 gtccacaaag tcctgcaatt cggcaaactc gatgatctcg tcgaaccgct cggacatata
     1261 cgcgcggctg tagccgagaa tcgcgccgtt gagatagatg ttctcgcgcg cggaaaggtt
     1321 ggcgtcaaag cccgcgccga gctcgatgag gggagcgatg ctcccgcagg tctccaccgt
     1381 gcccttggtc ggcttcaaaa tgccggagac gattttcaaa agcgtgctct tgccggagcc
     1441 gttcagcccg acgatgccga gagaatcgcc cttttcgagc gtgaacgata tgtcccgcag
     1501 cgcccagaac tcctgaaaat ggagctcccg gcgcacggcc ttgatgaaat attccttgat
     1561 gctgtcgatc ttctccgagc tgagattgaa catcatcgaa acatcgttga ctttaataac
     1621 gctgcccatg gcgcacctcc gctcagacat acaggacgaa cttgtcctgc tttttataaa
     1681 atacgaaaag ccccagcgcg agcatcaaaa gccccagaca caggcagaga aggttttcct
     1741 tcagactggg gaacacgccg tagagcacga tggaacgcat gtaggaaaca agatggtaca
     1801 tcgggttgat ctgcatgagc ttcatcacgg cgtccggcag gatctccacg gggtagaaga
     1861 tgggcgtgaa gtatgtccac gcgagaatga acacgctgta gaaatgcgcg atatcgcgga
     1921 aaaacaccgt gaccgccgag agcaggatgc cgagcccgac ggagaagagg aacgtataga
     1981 aaatggggat cgggagcagc aggagcgtcg cgtggaacgg cgcgtcggtg atgagcatca
     2041 cgatgaacat cgcaatgagc gaaaacccga gattcacaag ccccgaaagc acattcgaga
     2101 gcgggaaaag gtacttcggg atatagacct ttttgatgag ggaggcgttg gatatgatcg
     2161 agttgagcgc ggtcgtcgtc gattccgaat tgaacgcaaa gatgagcgag cccgaaagat
     2221 aatagatcgg gaaattcggg atattctgct tgaagagcgt ggaaaagacg atcgtgatga
     2281 cgaccatcat cagcagcggg ttgagcaccg tccacacgag ccccagcgcg gaatggcggt
     2341 agcgcacctt gatgtcgcgc accacaagct cgcgcagcag cggcgtgaac cggcgaaagc
     2401 cctccatttt ttcttttacg tagcctttgt tgaatgccat gatccggtat tcctcacttt
     2461 ttcgtatcgc ggctctgcag cttccactgc agccgcttta ccgagcgcat catgccgcgc
     2521 ggaacgcacg ccatgatgag cggtttgaca agatagatgt aatccgaagc cggggagccc
     2581 atcgcgcgca gtccgcgcgc gcggacgcga acctcataca tgcgggtcct ggcattgtga
     2641 cggagatagg cttcctgcgg ttcgtacacc gagtaaagga tctcctgcaa gttttccccg
     2701 atcacgccgt tggcggcaag ccgcatgacg agatcataat cctccgtgcg gcgcgtttcg
     2761 ggggaggtgc tgtagccgtt cacgctctcg agcacggcgc ggcggaagag catcgtcggg
     2821 tggaagaagg ggttcttctg cgcgatgatg tgcttcgtga tcttctgcgg aaactgccgc
     2881 gtgctccata cgccggtgtc gtcgaaaacg cgcacgccgc acgcggcgta gggcgcgccg
     2941 gacgagagca gaaagtccat cgtccgctcg atgcggtcag agtcggaaac atcgtcggcg
     3001 tcctgccggg cgatgtatgc gccctgcgcg cgcgcaaggc actcgttgag cgaagcgccg
     3061 agaccgagat ttgttttgtt ttggaagatc gtgacgcgcg catcctgcgc ggcaatgcgc
     3121 tgcatgatgc gccatgtatc gtccgtggag gcgtcgtcgc agagaatgag ctccagatcg
     3181 cggtacgtct gcgccagaac ggacgccgcc gccttttcga gcgtgggggc gccgttatat
     3241 acgctcatga gaacggaaac gagtccggca ttgcagttgg gcatttttcc cctcctccgg
     3301 gttagtataa acgtatcaga ggcataaaaa ccctttataa aggtaacact tttgcgcgat
     3361 gatagcaagc agtatgtttt tcctgtacgg aaaaaccgcc ggaaagagtc attttcccgg
     3421 ttgacaagcg gatatttcgt gatagaatgt ataagatttt tgggctatga cgaagcaagc
     3481 cgcgctgata cggtctacag agagaggggg aggctgaaag cccctcgtcc ggcgccgcgg
     3541 cagccacttc cgagccttcg cgcgaagagc gcggcggtcc gcctccgtta tcgggcgcac
     3601 gagatacggc tggcgtcagc gcttaaagcc gcaatcaggg tggtaccgcg tggaatgttt
     3661 ttccccgccc ctgtttttcg ggggcggggt gtatttgttt ttggaggtta gtatgaaaaa
     3721 tttcggactc aatgagctgc gggagatgta tctctccttc tttgagacaa aaggtcatct
     3781 gcgtctgccc agcttctcgc tgatcccccg caacgacccc tcgcttctgc tcatcaacgc
     3841 cggcatgagc cccatgaaaa catggtttac cggggaagaa gagccgccgc gccgccgcgt
     3901 gacgacctgc cagaagtgca tccgcacgcc cgacattgag cgcgtgggca agacggcgcg
     3961 ccacggcact tactttgaaa tgctcggcaa cttctccttc ggcgattact tcaagcgcga
     4021 agccatccac tgggcgtggg aatttctgac ctcccccgac tgggtcggca tcgatcccga
     4081 ccggctgtac ccgagcgtct atctcgatga cgacgaggcc tggaacatct ggcacagcga
     4141 gatcggcatc gccccggagc gcattttccg cttcggcaag gaggataact tctgggagca
     4201 cggcagcggt ccgtgcggcc cctgctccga gatctactac gaccgcggcg agaaatacgg
     4261 ctg
//
LOCUS       DAWAAE010000160         4256 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8226_length_4256_cov_3.588907, whole genome shotgun sequence.
ACCESSION   DAWAAE010000160 DAWAAE010000000
VERSION     DAWAAE010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_4256_cov_3.588907"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          783 a         1320 c         1375 g          778 t
ORIGIN      
        1 cacaaaaggg acgaaaggca tgcttccgcg gtaccaccca cattcggagc ttttaaaaac
       61 tccgctctcg tgcgctgtaa cgggcgcagc ccgccgggga ttggccggcg ctcacgggcg
      121 aactatacgg agccttttcc gcggggcgct ctcagccggc gacgcccttc tctgctgcgg
      181 cggtattccg tcatattccc gatcaacgcg aaataacata ctctttatac catagtgcgc
      241 gccggttcgc aagaataaat ttgacgaatg gcgcgcggga atctataatg tattttggaa
      301 ttttatcgga gagggtgaaa gccgtggacg ggaatatgcc ggagaccgtg cgcgtatact
      361 gggatcgcgg cggcgtgtcc gtgccgcggc gccgagccga aacgcacaag ggcgattacg
      421 gaaagctcct catcgtcggc ggaagcgtcg ggtataccgg cgcgccgaat ctctgcgcgc
      481 gctcggcggt gcgctcgggc gcggggctcg tatatctcgg cgtgccggaa gcgatctgga
      541 acgtatgcgc cgtgaaaaac gacgaggcca tgccgtttcc tcttccgtgc gatgcttccg
      601 gaaagctcac cgcggacgcg ctctctccgc tgcgggagta ttatgaccgc tgcggcgtgc
      661 tcgcgctcgg ccccggtctc ggccggagcg atgggacggc ggctctcacc gccgcgctca
      721 tccgaaagtt tcccggaacg atcgtcctcg atgcggacgc gctctgggcg gtatcccttg
      781 taccggacat cctgcgcgag gcgaagagcg agatcgttat cacgccgcac gagggggagt
      841 tcctccgtct cggcggctcg ctggaaaacg ggcgcgagcg cggcgcgtgg gagtttgcgc
      901 ggcggtacgg ctgcgtcacg gtactcaagg gccaccgcac gatcatcgcg caccccgccg
      961 ggcggatgta cgtcattgag gcgggcaatc ccggcatggc gaagggcggc agcggcgacg
     1021 tgctgaccgg cgtcacggcg gcgctgcttg ggcagatgga gacggagagg gcggccgtca
     1081 ccgcgtgcgc cgtccacgcc ggggcgggcg acgtgtgcgc gcaaaagctc ggcgaatacg
     1141 gcatgacgcc ctcggacatc atcgaagagc tgccgtatat tttcaaaacg atcacggagt
     1201 aaagatcatg aagcggacga ttttgttcgc actgatgata cccctgctgc tcaccggctg
     1261 cggtgcgcgg cgggacgcgg agaactggaa agcgtttgcc gagacggtcg agaccgcgga
     1321 gcggatatcg ttccacgcgg agatcaccgc gcactgggag gacagctccg cctcgttcgg
     1381 cgcggacgtc gcgcgtgcgg gcggcgaaac gcacatcacg ctcaccgcgc cggagaccgt
     1441 ttccggcatc accgtccgcg caagggacag cgagacggcg gtggagtttg acggcgtgat
     1501 tctctcgctc gacgccgggc ggagcgaaac gctctctccc tgcggcgcgc cgcagcgcat
     1561 gctcgccgcg ctcacgcagg gcacgatgga atactgcgtc tcgtcctcgg cagccttcac
     1621 cggcccggac ggcgagagcg caacgctctg gcgggacgaa gccggcgcgc tcaccggcgc
     1681 ggagatcgcc cgcgacgggc ggaaaatcct gacgctggag atcacggatt gggtcatgga
     1741 gtaaggctcc cttgtgtaaa gggagctggc agccggcagg ctgactgagg gattgtcctt
     1801 ttcggctttc tgaaaatccg acaacccctc cggcaaaaca gaaagttttg ccacctcccc
     1861 ttacacaggg gaggcttcaa gcagaaagga gcatcctttt atggatacac gaaagacaag
     1921 gacatgggcg gagatttcgc tcggcgcgct gcggcacaat gtggaggaaa tgcagcgcgc
     1981 gctgccggac ggctgcgggc tgctcggcgt cgtaaaggcg gatgcctacg gccacggcgc
     2041 ttccgagatc gccgcggcgc tgcgtgagtg cggctgccgg tatctcgccg tcgcctgccc
     2101 gcaggaggcg cttgctctcc gtcaagccgg agagacgatg cccattctca ttctcggcgc
     2161 ggtggacgcg gcgttcgcgt ccgacatggc ggcgaacgac atcacgctct ccgtggaatg
     2221 tgccgaaaag ggccgcgccc tctccgcagc gctgcgcggc ggcgagcggc tccggatcca
     2281 cctgaagctc gacaccggca tggggcgtct cggctttcgc gtgcaggacg agaccgcgct
     2341 gcgcgaagcc gccgcggtag cacagctgcc gaatctggac gccgaggggg ttttcaccca
     2401 cttctccgtt tccgacacgc ccggcgagga ggaatatacc gccgcgcagc tcgcgctgtt
     2461 tctctccgcg gtcgaaaagc tggaagccat gactggccgc cggttcccgc tgcgccactg
     2521 cgccaactcc ggcgcggtgc tttcgtggcg cgataagggc gcagcgctcg atctcgtccg
     2581 ccccggcctt ctcacctacg gcgtttatcc ggacagcgag cgcggcgggc tttcgctcac
     2641 gccggtcatg gcgctcaaaa gccgcgtgag cgcgatcacg catcacaaga agggcgactc
     2701 gatcagctac ggccgcacat ggacggcgga gcgcgactgc acgctcgcgg tgctccccgt
     2761 cgggtacgcg gacggcttgc agcgctgcct ttccggaaag ctcgaggtgc tgctgcgcgg
     2821 gaagcgcgta aagcaggtcg ggcgcatctg catggacatg tgcatgctgg atgtgaccga
     2881 tattccggac gccgccgtgg gcgacgttgt cacggtgttc ggctgcgggg acgacggcgc
     2941 gccgacggtc gcggagcttg ccgcgctcgc cgggacgatc ccctatgagc tgctctgcgg
     3001 cgtatcgctg cgcgtgccgc gcatttatac ggaaaattga cctctttcct ggcaaaagaa
     3061 gggatggccg cgcgccatcc cttctttctc tttattcggc attcttccgc agcaggactg
     3121 tgagctcccc gctggtacct gtgatctcac accttgtccc ctccgggacg atggcgagca
     3181 ccgtttccgc cgtatacgga tggaacacca tccccttcca tttcccggcg gtatacacgt
     3241 agtctccgtc cgcgtttaca cggtgcggtt ccgtccggta ttcttcgtcc agcgggtcca
     3301 gcgtgaagat cacaatgccg cccggctttg tgatgcggca gagctccttt accgccgcgg
     3361 cagcctccgc gcggggcaga tgatccagaa cgtcccggca gaccacgccg tcaaatgtcc
     3421 cgtcggcgta tccggtcgag aggataccgg cggttttcag ctctgccgcc aacgccgctt
     3481 tctccagcag ggtctgcgcg atctccacgg cgtgcgatga aacatcaaag ccgcagacag
     3541 aaaagccgtt tgcggcgagc ttcatcgcat acgccccgca gccgcagccc gcgtcacaga
     3601 cggttttgac gccgtgccgg tgcagcgcac tgatcagcgc atccggtgcc atatcaatcg
     3661 attgaacata tcccgcgacc cgctccggat cgacccgttt ccaggagtgt tcccaaaagc
     3721 gttccatagt atttctttct ctcctactgc ccggaataaa aacccaatcg aagcccagcg
     3781 gagcgggttc gattcggaaa aggaagaagg agccaacata gtgcagtttt cgccgtgagg
     3841 cggaaacgga acggtgttgg ctccttctga cgtcctggca cgcctgcccg ggctcgaacc
     3901 ggggacctcg ggcttaggag gctcgcgctc tatcctactg agctacagac gcatacggtt
     3961 tatgggtgca gaaactgcgc gatagtattc tatctcttga tgtgcgatgc tgtcaagggg
     4021 gcattccgtc tgcggcggcg cgcctttttc aggaaaaggc gttgcaatcg tattcatcgt
     4081 gtgatattct tggcttacat ggatgaatct cccgtaaaat tgaatagaaa catccgaatg
     4141 aaggaaccgt gtgtgatcca cggactgtaa cggcaactgt aacgcggagc gaccccaata
     4201 tgccactggg aaaccgggaa ggcgggcgag cgaggaaacg gagtcaggat atttca
//
LOCUS       DAWAAE010000161         4185 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8356_length_4185_cov_2.772397, whole genome shotgun sequence.
ACCESSION   DAWAAE010000161 DAWAAE010000000
VERSION     DAWAAE010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_4185_cov_2.772397"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3779..3814
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a         1286 c         1238 g          901 t
ORIGIN      
        1 atcgtctctt cgagctttgc gctcgtcatg ccgacgcgcc ctttggcgtc ctcgatgatc
       61 tcctccggca gaccgagccg ccgggagatg gcaaaggcgt tggattttcc cggaataccg
      121 agaagcagcc ggtacgtcgg gcgcagggtg tccacgtcga actcgcacga ggcgttcata
      181 acgccggacg tgttcgtggc gtatacctta agctcggcat agtgcgtcgt tgcggcgatg
      241 atgcagccgc gcgcgcgggc atcctccaaa atcgacacgg caagcgccgc gccctcgacg
      301 gggtctgttc ccgcgccgag ctcgtcgaag agaagaagcg atccgtcgcc gcactctttg
      361 agcacgttca cgatgttcac catgtgcgcc gagaaggtgg agagcgactg ctcgatgctc
      421 tgctcgtcgc cgatgtctgc aaggacttta tcaaatatgg gaacgcagct tccctccgcg
      481 gcgggaatgt gcaggccgca ctgcgccata aggcagagaa ggccgatggt cttgagcgta
      541 acggtcttgc cgccggtgtt cgggccggtg atgacgagcg tatcgtaatc ctcgccgagc
      601 tccacatcga tcggcacggc cttgtcctta tcgagcagcg gatggcgcgc ccggcgcagc
      661 cggacgccgc gctcggagag ctccggcgca ccggcattca gcttgaagct gagctttgcc
      721 cgcgcgaaaa tgccgtcgag ccgcacgaga gcggagaagt cggcgtcgat atcctcgcgg
      781 tgcgcggcgc actcggcgga aagctccgcg agaatgcgct cgatctccgc tttttccttc
      841 gctttcagct cgcggatctc gttgtttgcc ttgaccgccg ccatcggctc gacgaagagc
      901 gtggcgccgg atgccgaaac atcgtgcacg aggcccggaa cgctgccctt gcattcggcc
      961 ttgaccggaa caacgtagcg atccgagcgc gtcgtgatga tcgggtcctg caaaaacttc
     1021 gccatggacg gcgcggagat gatcttctgc aggctgtcgc gcacgcgagc cgccgccgcg
     1081 cgcattttgc ggcggatgtc cgaaagctca ctgctcgcgc tgtcggcgat ctcatcctcg
     1141 ccggggatgg agtttacgat cttgtcctcc agaaaccggt tcgtctggag cattttgaaa
     1201 agatggtcga tgcacgtctc cttctcggcg ctcatgccgt aggagcgcac ggcgcgcgcg
     1261 ctttgcagca cgcccgcgat ctcgagaagc tcgcgcgtat tgagcacgcc gcccatgtcg
     1321 gcacgggcga gcgatccgcg cacatcgcgc agacccgaaa gcgacgggct gcccttatag
     1381 gccatcatgt cctttgcatc gctcgtctcg gcgagcagcc gttcgacctc cgcgcggtgg
     1441 acgctcggca cgagagcgag cgcctcggcc ttcgccggtt cggacaccgc ctcccccgcg
     1501 agcatttcca gcaccgccgg aagttcgaga gtatttcttg atttttccag taagtccatt
     1561 tatctctccg tttgcatcat gtgtttacat aatttgtttt acgtaatttc attaacataa
     1621 atcgttttgc ataattcgat ttacatattt cggtttacgc aattttatta acgtaatata
     1681 gttaacataa atatattatc gttcaccgat tttgcagcga gtgccagtaa tcgagcgtac
     1741 cgaagccgcg gtcgagctgc gcgagggtat attcctcgca ggccgtgcag aaacgctcgt
     1801 ccgacgactc gtccagcgtg aaggaaaaga tcttcttctc ccccgccgag cacacgtgac
     1861 gcatggcagc gagcgacgcg ccgcagagca gcaccgactt tcccggcgcg ccgggcgcgc
     1921 agccggtgca gtggacgccg ccgccgtaga gactcaggcg cggcttttcg ggcgtgtgcg
     1981 aaccgcagtc cgtgcagcgg tcgagctgtg gagcgaaccc cgcaagacac atgagccgca
     2041 gctcaaagac cgccttgata tgtttggccg gatatagctg ccgcgagagc gcgaacagtg
     2101 cgttgagccc cagatgcagc agcgccgtgc tgtggctgtc ctcgtccgat acggcctcca
     2161 gaagctccgc catatagcag ccgagcgcat agtcgccgag atcggcgcgc agcgccgcga
     2221 agtcctccag cacgctcccc tcggtcagcg tataccggcc gcggttttca aaaaatgtca
     2281 ggtccgacca cgtgagcacc tgcgacgctg ccgagcactt tgagcccttg cgcagcgcgc
     2341 cgcgcgcctc cgcagtgatt ttcccctcct cctccgtgag gatggtgaga atcttgctcg
     2401 actccttgta tttcacctcg cgcagcacca gtccgtgcag ggttttgtac attatgtaaa
     2461 cctctcgtct cacgacagag gccgtgaggg cgctctgtcg ttcgtcttct gttctttctt
     2521 tttgttctcc gccggagcga tgcgggtcag cagccagcag agcgggtcgc cggtgtcgcg
     2581 gaacacgtcc cagaaaatct tcgtatccat acggggagtt ttcccctgcg ccgggcggtt
     2641 atgaccggga atttccggat atggcggcag tttcaggtat cggtatagcc aaaattgcgc
     2701 agcagcgaat tggaatcacg ccacttttcc tttaccttca cccacgtctg caaaaacacc
     2761 ttcgtgccca taaattcctc aatgtccttg cgggcgtcct cgccgatctt tttgagcatc
     2821 gcgccgtgct tgccgatgat gatccccttg tggctcgcct tttcgcagta gatcgtcgct
     2881 tccagatcga tgatgccgtc gtcccgctcg gaaaagcgcg tgatctccac cgccgtgcca
     2941 tgcggcacct cgcgttcgag attgagcagc agcttttcgc ggatcagctc ggcgcagacc
     3001 tgcttttccg gctggtcggt cgtcacgccc tccgggaaga gcgccgggga ctccacggcg
     3061 aacttatcga gctccttgag aagctcctcc acgccgtcgc cggtcttggc ggagatgggg
     3121 acgatggcgt taaaggcata agcctcgctg tagagcgcga tgacggcaag gagcttttcc
     3181 ggctcaacgg tatcgagctt gttgatggcg agcaccgccg ggcagtttgc cgccttgatg
     3241 cttgcgatca gctcgcgctc ctgcggcccg agagaggcga ccggctccac aacgagcacg
     3301 acggcgtcca catccgcgac gctctggcgc acgacattca ccatatagtc gccgaggcgg
     3361 gtgcgcggct tatggaagcc gggggtatca agaaatacga actgcgtctc cccgcgctgg
     3421 cacacggcgg tgatgcggtt tcgcgtcgtc tgcggtttgt tggatacgat ggcgatcttt
     3481 tccccggcga gagcgttcgt cagggtggat ttgccgacgt tgggccgtcc gcagatggtg
     3541 atcatggcag ttttagaaat catagccgag ctccttcata atggcttttt ctcttgcgcg
     3601 cattttggct ttttcctcgc cctcgtcgag atggtcgtaa ccgagcagat gcagtacgga
     3661 gtgcaccgtg agatactgcg cctcgtgctt aaatccgccg ccgtactcct cgccctgcgc
     3721 ttcggcacgc tcgagcgaga ggaccatgtc gccgagatag cggcggttcg tgtcgtaann
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtcca cgccctcggc gcgcagcgct
     3841 gcggcggcgg ccttctttac aagcgcggcc gtctccgggt ggccgagacc gcgcttttcc
     3901 cggtaaatgt agaatttatg ctgcatcgtt atttcctctt gtagttattg tatccgctat
     3961 tgtcccggcg ctgcggcgcg gcggttttct tctcccgctc cgcggcggca gcggcggcct
     4021 tttcgtgctt ttcataggcg gcgatgatct gctgcacaag tgcgtggcgc acaacgtccg
     4081 cgccggtgaa gcggcagatg gcaatatcgt ccacgccgtc gagcaccttc atcgcttcct
     4141 tgaggccgga cgccttgtcc gccgggaggt cgatctgcgt cacgt
//
LOCUS       DAWAAE010000162         4162 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8409_length_4162_cov_3.241782, whole genome shotgun sequence.
ACCESSION   DAWAAE010000162 DAWAAE010000000
VERSION     DAWAAE010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8409_length_4162_cov_3.241782"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          801 a         1314 c         1209 g          838 t
ORIGIN      
        1 gccttggaga gaatgatctc cggcacatcg gtcacgccga gccagtagtt ctcgttcgcg
       61 tcaagataca cggtgcgcag gtcggtctca cggtaggcgt attcgcccgc gccgaccgcc
      121 ttgccgttgt ttgcgcgtac ggaggagaga ttgcccttcg tgaagccgaa ggaaccgtta
      181 tccggatcga agaggctcac gtcaccgtaa acgtgcagcg gcgcgatgca taccccggtg
      241 agcgttttga ggatggtatc gtcgcagcgg gtgagcgaga tgcggatcgt ccggtcatcc
      301 acctttttca cgccggacac gctctgcacg ccgccgcgcc agtcatcgtc cagcggcgcc
      361 cattcgcgca cgagagaatt ctgcaccgcg gcgagcatat ctgcgcggcc gatgccctcg
      421 atgctccagt actgcgccac atcgccgtcg taggcatcat acatttcgtt atagagatcc
      481 tccgccgttg ggaacgttgt cgtgaggtcc cacgtcgttc cgctgttcgc ggtaaacccg
      541 ccgttatcgt caaacttgcc gaagccgcgg tcgagcatcg tatacgccac ctgcagaccg
      601 gtattgccga gcacttcctc gcgggtgtac ggcgtgcgca gcgcgatggt gccgctgtag
      661 ttttcgatcg tgtagtccac gagcgcctgc gcatcgttgc gccaggcgtt gtcgatcgcg
      721 tcgcggatct cgtcggcgcg gacggtgtcg tattcgtcga gtgcctgctg cgccttgacg
      781 acgtcggcgt cgctctccac gttcgcctcg ctcacgccct tttccatggc ggcgcgcttg
      841 gcgtcctcca cggccttttg caggtcggac tcatacttgc cgccgttata gatctcgtca
      901 tagatcatca tgtacttgcc gtacatgccc gaatccatgc cgttccaata gagcgcgatc
      961 ccgtcaatgg ggagctgatt gatcgtgtag gggccgtcat agctctcatc catgaggaca
     1021 taataggtaa agagaagatc gtccgccgtc agctccacgc cgtcggcaca gagaaggccc
     1081 ttctcaagac gaatcgtaac ggttaccgtt ccgtccgtcc ccgtcgtgcg ggtgatctcg
     1141 gcaggggcgc ggttgccctc cacagcgcgc agagagagct gcgtcatttc agcgacggcc
     1201 ttgtcgccgt ccgcctcggc aaagaacggg ctgaacacgc cgtccatttt ttcataggcg
     1261 atctgtctcg gctccggcgt cacctccggc gcatccgaaa agacgagatc cgaaccgggg
     1321 acgggcgtct cggtcggcgc gatcacgctc ataacgccat cgaccgtcag cgaacagccg
     1381 gtgagcgtga gcaggatcgg tatcagcata gccgcagctg cggcccgaat ggatttcttc
     1441 ataccaatgc tcctttcgtt cttattaaaa tatcataatc cgctttttct gtcaatttgt
     1501 gttatgtaaa ctattttaac gttatgtcaa ctttcgtccg catccgcacg ccggttcttt
     1561 tatactgaac gcataaggac acgagagggg cggcgcgctt ggacggaaaa ctcacgcagg
     1621 aggaagctct ccggctgctc cgggcgcgga cgctcagcgt ctgggaggac gcggctcttc
     1681 tccgggagta tatacgcctt tgcggtataa cgcagtcgga atgcgcggtt cggctcggaa
     1741 gatcgcagtc gtcggtggca aaccgcctgc ggctcctgcg ccttacggac ggagagcgcc
     1801 gcgccatgca ggaggccgga ctttcggagc gccacgccag agcgctgctc cgtctcgggg
     1861 acgcagcaca gcgtcaggcg gcgctgcgga ccgttttgca gcagcgcatg agcgttgcag
     1921 agacggaagg gtatgtggag acgctgctcc gtccgccgga acgtcgggag ctcggcgcgc
     1981 tgctttcgga ggtgcgccgt ctgctggacg ccggcgcctg cacggatgcg gaagagtccg
     2041 caggccccgg cggactctcc gttacgctct tttttccggc aaacgatact tttccatgat
     2101 tttatgcttt acaagcggcg gggaatgtgg taggataatc gagcgtttga ccgccgcgct
     2161 cagtttccgg ccgcctttca gccgatactt agccgcgcgg gaagcgaaaa tcaggaaagg
     2221 atcgaagtta ctatgatcac gaaagaccag aagaccgtcg ttatcgacgc caacaagacc
     2281 cacgagggcg acaccggttc tccggaagtc cagatcgcca tcctcaccga gcgcatccgt
     2341 cagctgacgg agcatctcaa gaagcacccc aacgacgacc acagccgtct gggcatgtac
     2401 aagatggtcg gcaagcgccg ccgtctgctg gagtacctgt cccgcaagga catcgagcgt
     2461 taccgcgcca ttatcgcgaa actgggtatc cgcaagtaat tcgcggagac ctgccgaggg
     2521 ttatgaaggt tttttacgga gggacaagtg aatactgtcc ctccgtattt tgtttcaaac
     2581 gaaagccgca ggctttcgtt tgagaaagat tgcggctcgc cgcagtgcac gctcttcgag
     2641 cgcacgtttg cgagactttt taatttgtgt accaatgtgt gtgaatatat aaaaggagta
     2701 acgaaagaat gatcatctct cacaaggaat ttccgaattt caagcgttac gaaatggact
     2761 actgcggccg tccgttcttc atggaggtcg gcaagatggc cgagctctgc aacgcttccg
     2821 ttctcgtcgg ctacggtgac acccgcgttc tcgtgaccgc caccgcgtcc gcccgcccga
     2881 aggacggcat cgactacttc cctctcgccg tggacttcaa tgagaagctc tacgccgtcg
     2941 gccgcattcc cggcagcttc aaccgccgcg agggccgtcc gtctctgaac gccgtgctcg
     3001 cgagccgtct catcgaccgc ccgatgcgcc cgctcttccc gtccgacctg cgcaacgacg
     3061 ttgtcatcgc ctgcgaagtg ctcgccgttg accgtgactg ctcccccgag gtcgccgcga
     3121 tgatcggcgc ttccaccgcc gtttccattt ccgatatccc gttcaacggc cccatcgccg
     3181 gcatcgtcct cggctgggac ggtgagaagt ttctcttcaa ccccaccgcc gccgagaagg
     3241 aaaccaaccg catgacgacg accatcgccg ccacgcacaa gaagatcgtt atgatcgagt
     3301 ccgaggctga ccaggtcccc gacgatgtca tgtacgaggg catcgtgcag gcgcacgaga
     3361 tggtgcagcc gatgctcgat cttatcgacc gcatggtcgc cgagatcggc aagcccaagt
     3421 tctcttacga gcacgccgcg ttcgacgacg agctttttga aaagcttgtc gagaacgagt
     3481 ttgccggcat ggagtactgc atggacaccg acgacaagaa cgtccgcgaa gcccgcgtga
     3541 acgagtgggt caccgcgatg caggaaaagt atgaggccga ccatcccgag atcatgcagt
     3601 acatggacga gatcctctac aagctgcaga agaaggtcgt caagaagtgg ctgctcgccg
     3661 gcaagcgtgt ggacggccgc gccatgaacg agatccgtcc gctcggcgcg gaagtcggcg
     3721 tgctgcccgt tgtccacggc tccgctctct tcacccgcgg ccagacgcag gtgctctcca
     3781 tcgccacgct gaacacgctc tccgccgccc agaagctcga caccatctgg gacgagaccg
     3841 agaagcgctt catgcaccac tacaacatgc cctcctactc caccggcgag gcgcgcgccg
     3901 cccgcagcac cggccgccgc gaatacggcc acggcgctct cgtggagaag gctctccagt
     3961 ccgtcatccc gtccgtggag gacttcccct acgccatccg cgtggtgagc gaagtgctct
     4021 cctccaacgg ttccacctcg cagggctcca tctgcggcac gacgctcgcg ctcatggacg
     4081 ccggcgtgcc tctgaaggcc cccgttgccg gcattagctg cggccttatc caggacggcg
     4141 acaacttcac cacgttcatc ga
//
LOCUS       DAWAAE010000163         4149 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8441_length_4149_cov_3.360039, whole genome shotgun sequence.
ACCESSION   DAWAAE010000163 DAWAAE010000000
VERSION     DAWAAE010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8441_length_4149_cov_3.360039"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          856 a         1206 c         1313 g          774 t
ORIGIN      
        1 acgtgcgagc gaagcgagtg cgctgcggcg agccgcagcc ttttcaaacg agagcgtaag
       61 cgaagcgggt ctcgtttgat attcgtttga cattttctga tttttgtttc ttccctccgg
      121 cgggattttc ggtataatgg ggaagtaagc aaaaacagag gtgatcgttt atgagatatt
      181 gggaaattct ggttttctgt ctgggtatat cgctggacgt gttcggcgcc gccgtgtgtc
      241 agggcgcggt gctcggcgag cttcgccgcc gccgcatcgc cgcgtactgc ctgatcttct
      301 gcgcggcaca ggtcgcggcg atggagatcg gccggctgct cggcatgctg ccgcgcttcg
      361 catcgtacta taccgtggcg caggggctgt ggcatattct cggcgcgctc attttctttg
      421 tgctcggcgt gtgtctgatc gtcaaggccg tccggaaaaa gaccgtattc gagcaccgct
      481 ccgagatcac ttacaaaaaa gtcgtcggca ccgccgtcct cacgagcctt gattcgctgc
      541 tcgcgggcgt cagctcccgc tttctcgatg cgtactggct ctcctgtttc gccacgctct
      601 tcggcgtgac ggcgttgtgc gcgctcggcg gcgtgtcggt ggggtatcat ttcgggtatt
      661 cccagaaaaa caccgcgtac tggatcggcg gagcgctgtt tcttgtgacc ggcgcggatg
      721 ttctgatccg cttgtttgtg tgaaaaggag acacatatgg acagccgcaa ggatatgaca
      781 aagcgcctga tcgccgacgg cttcaaggcg ctgatgctgc gatatccctt tgaaaagatc
      841 tccattatga tgataacgaa cgaggccggg atccgccgcc cgtcgtttta taatcacttt
      901 caggacaagt acgacctgct cgcgtggatc gtggaaacgg acgtgatcgc cccggcgggc
      961 gagccgctgc gccgcggcga cggggtcggc gcgctgcgca cgatctttgc cgggctcgtg
     1021 gaggatgccg ccttttaccg caaggcgttc accgtcacag ggcagaacgg ctttgaggat
     1081 tcgttcaccg acgcggtgca gcgtctgctt ttggaaaacg ccgcgctcac gcccgccgcg
     1141 gggtatgcgg gactgctctc ggaaaaaacg gtggcgcgct tcagcgccat ctgcctcgtg
     1201 tccacggtga agcactggct ggaaagcccc cgcgcagcga gcgtggacga gctggtggac
     1261 gcctacctct atctcgtatc ccacagcgca tggatgtaaa gataataaag cacagcggcc
     1321 gacgtgttga tgccggccgc tgtgcttttt ttatcccatc cggacgatga gcatatcgcc
     1381 cgcccggcaa atgctgccct ccgcgggaaa cggcggcatt gcatccggct gcgctccctc
     1441 ggggagctcc gattcgctgc cgatgcggat cggggtctgc agaatgtacg aaaaatacgc
     1501 ggcgtaatac ccgccgccca ccgtgctttt cacccacgcg ccggtgatct gcgatacgct
     1561 gccgagcgct tcgggcgtat cgaaggacgg cagaccgata aacacgaccg gcgtttctcc
     1621 ggggacatac ccctcggtct ggtccagacg gaggcagagc tccgtcatgc gggaaagcgt
     1681 ggcctgccgc tcaaggtctt ttttgagata gagcgcgttt gccgtctgga tatttcccca
     1741 gacgagcgcg gcgatcagcg caagagaaac ggcggcacag ccggagaata cccggctttc
     1801 cggcaaactt tgcaggcact gccgcagcag caggatatcg aagacataca ccagacagaa
     1861 ggcgtacttc atcagatcgt gcgcctccat cccggcgtag ccgaggaagt tggaaagatt
     1921 catgcccatc ggcaaaagcg cggcaagaac gagcgcaagg agaagattgg ccgcggggag
     1981 ccgttttcgg aggagaaaac atgctgccgc cgccagcgtg agaacggcga gcaggagatg
     2041 ccccgcgcgc gtgaccggca gaggaacgaa gaccggctgc cgcacggtac tgcgtgccca
     2101 tgtgtcatag gttgatcgga cggcttccgg cagaaggtcg ggcgtcatcc ggccgagact
     2161 caaaaggctg ttgtacccgc cgtcgagcgc gacgcccgat gcgctgcaca cggcgcggac
     2221 gagaatatag tacgcggccc cgccgataaa aagaatgccg acggctttga gctcgtcgaa
     2281 aaataccgct ttcgcgctct cgttctccag aagccgcaga atacaggcga tcatgacaag
     2341 cgagaccgtg accgagatca tcgcctgata gagcgcgagc gagccgacaa tggggataac
     2401 ggcgagcgcc ctgcgccgga gcgtgccgcg gagaaggaga tatgccgcgc acacggcgag
     2461 cagcatggcg agcatatcgc cgtccagatc gtagatgtac gttgccgcca gcgcggaata
     2521 tgacacattg gtcacgaaaa gagcgccgat cacggcaagg agcagcgcgt tgtccacgga
     2581 gaacagccgc gaaacgagat acaccgcaag cgatacccac acgagcgaga gaacgccgga
     2641 gagccacggt gtaccggcac cgccgctgaa cacggtgcgg tacgccggat agagaaaccg
     2701 accgagcgat attttccagt ccgccaccga gccggcggca tagcggatat ctccggaaag
     2761 gaacatctcg ttgagcgagt cgtggctgac ggtaaaattg gtgaacgcat agccgtgcgc
     2821 gagcaggcca aagagaaaaa cggcggcaaa ggaagcgaga agccgttggc gctcgccggt
     2881 gatgcgaaga tgcggcgcgc ggcgcagcgc ggaaacaaat ctggtcatgg cggaaaaacc
     2941 tcccgcggaa aggcggtcac ggattcacaa aaaggtgttt gcggttgttg aaatacggaa
     3001 tattgacaaa gagcatgacg agcgaaccga tgatgctccc ggcgacttcg gcggtaaaga
     3061 ccgactggcc gataatgatg cttccgatga gcagatagag caccgttacc gcggtattga
     3121 cgatgtacat cagataatac atcatcgggc ccttggcgcg gaactttgcc agcgctctct
     3181 gcacgaccac cgcgtacacg gcgagcgcaa gacagacaac acccataacg atatccagcg
     3241 gctgcagcgc ggggaagacg cggtagacca tttcggcgtt cccctcataa tacgcgccgg
     3301 tgaagtactt tccggccgtt accagattga cgagcatacc ggcccacagc tgaaaataga
     3361 tgatgaactt gaaccacttc atgccgcgcc ggggcgctgc aatgacgccg aacgcggcgg
     3421 cgggatcggg cgtgccctgc ggcggcatgg ccggttcgac cggagctgcc ggcacagcgg
     3481 ggaggacggc gtgccttccg gcggcgcggc ggcgggagcg ggggcaagat cggcgccgca
     3541 ttcgggacag aatttcgcct ggtcggggat ctgttttccg cagaatcggc agtacatggg
     3601 gattcctcct tctgtttatt tatatttaat acgggggtta attccatgtt ccttcgttgg
     3661 cggtgaaaag ctcagcaatg cggtttcgga gcggggcaag ctcctcgccc gcaagcgccg
     3721 gatttttccg gcagagcgct tcggcggcgg actgcgcctc gtgcagcatg tccatgctgc
     3781 cgagaaggtc cttgacgtgc atttccggca gaccgtgctg gcgcgagccg aaaaagtcac
     3841 cggggccgcg cagctccaga tcggcctgcg cgatctcaaa gccgttgttc gtgcgcgtga
     3901 gcacgcgcag ccgctccctg gattcttccg tcggcgtatc ggacacgagc acgcagtagc
     3961 tcttcgcctt gccgcgcccg acgcgtccgc gcagctggtg gagctgcgat agcccgaact
     4021 gctcggcgtt ttccacgacc atgagcgtag cgttcggcac gtctacgccg acctccacga
     4081 ccgtcgtgga aacaagaatg tccgtctctc cggcggcaaa ggcggccatg accgcttcct
     4141 tttccttcg
//
LOCUS       DAWAAE010000164         4014 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8755_length_4014_cov_2.916646, whole genome shotgun sequence.
ACCESSION   DAWAAE010000164 DAWAAE010000000
VERSION     DAWAAE010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8755_length_4014_cov_2.916646"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          793 a         1209 c         1136 g          876 t
ORIGIN      
        1 ttgacttcct caccgagaaa tacgaagcgg tcgttcagca ggcgggaaaa aatgtcatag
       61 gagcgttctc cgcgggacgt ctgttctaca acatacggta cgagactcat gaaataaatc
      121 ctcctttgcg gggttaaaat gcgatgatac gggaaagggc ggatcgccct ttcccgtgta
      181 tgtcagttta ccgtggggag acggatcact cctccgtctt ctcctcggtc ttggtgctct
      241 tgcgggtggt cttctcggcg ggcttcttct tggccttgga cgcctcctcc gtcttggcgg
      301 gctcctcggc cttcttctcc acggcaacgc cgctctcgga gatgatgctc atagcgcgct
      361 cacggcgcag gtcagcggtc agagcggcga catcgatggc cttcgccacg tcctcggcct
      421 tcatgccgta ggcttcaccg agcttctcat actcggcctg cagatcctct tcggagatct
      481 ggacattctc ggcttcggcg acggcggcga gcaggacctc ggtgcgggcc tgcttttcgg
      541 cggtggcgcg catgctctcg cggaaggtct tcatatcggt gccgatcatc ttgagataat
      601 cgtcgatgcg gatgccctgg ttggccatgt actgggcgta ctcgcggaca atgccgtcca
      661 tacgctcttc gatcatgcct tcgggcatat caacggtcat gctctccaca gcctgatcga
      721 cgagggcgtc gtcaaaggca ttcttcgcgg ccttttcctt ctcctcaacg gacttggcgc
      781 ggatgtcggc cttgagatcg gcgagcgtgt caaagtcgtt gtccttcgcg aactcgtcat
      841 ccagctcggg cagctcttca aacttgacct cgttgcactt gacgtggaac acgacggcct
      901 tgccgccgag atcgtggccg tagttctccg ggaaagtaat gtcaagatcg cgctcctcgc
      961 cggcggaaat gccgacgagc tggctctcaa agccgggaac gaaggtgtta gagccgagaa
     1021 cgagattctg gttctccgcc gtgccgccgt cgaacgggat accgtcgagc atgccggcgt
     1081 agttgatgtt cacggtgtcg ccgtcctgcg cgggacgctc cacggtgatg aggcgggcgt
     1141 tctgcttgcg caggcgctcg atgtggccgt caacctcttc gtcggtgact tcaacggagg
     1201 gcttctccgc ctcaaggccc ttatactggc cgagggtgac ctcggggtaa acgtcagtgc
     1261 tgaagctcag aagcgcgccg ttctcctcgg tgaccttgga atcggtcatg gtcggacggc
     1321 cgacggtctc gagcttgccc tcggcgacac cgaacgcaaa agcgtcgttc gcggcgttat
     1381 caaaggcgtc atcatagaac acgtccttgc cgtacatgcc ctcaatgacc atgcggggcg
     1441 ccttgccctt gcggaagccg gggaccatga tgcggctgcg agccttcaga tatgcaccgt
     1501 tgacatactt ttcaaactcg gcggcgtcga gctcgacatc aaaggtaacg gtgttttttt
     1561 ctttcttgct gttcttgaca atcatgtggg ggttactcct ctctatattt cagccgggac
     1621 tgaatatgca aatttcaagc cgatacatta tagcaaatca aaatgcaaat tgcaacggat
     1681 tatctgagct tatacggaac aaaatcaaga aaatctgcgg aaacaagacg gtaccggacg
     1741 ccctcgctct cgccgatgaa cgcgctgcgg cagccgtagc cgtgaatatg gccgtaacag
     1801 cactcatgca cgccgtattc ggaaagcaca tgcagtatct ccggacaggt ataatgaaga
     1861 tacagcggcg gataatgaag aaagacgagc ttttcccgct cgccggcgca tttgagcgat
     1921 gtttccagcc gcccgacctc gcgccggagg actttggcgt catgctccgt gccggtctct
     1981 tcgtctttaa accagccgcg cgtgccgcac agcgccagat tttcccattc cgcagagtta
     2041 ttatgcagca aagagatggt gtccaaaccg ttttgcacaa aaaaagcgtt catttttgcc
     2101 gcggtcgtcc accagtagtc gtgattgccc tttaccatca gctttttgcc gggcagactg
     2161 ttgaggaatt gaaaatccgg cagcgcctcg gcaagactca ttgcccagca gaaatcgccg
     2221 cagatgacgc aggtatcgtc ctcatgcagc agatcaaagc cgcggcggat cttgtcaaca
     2281 tagccgttcc aacgtccgcc gaaggcttcc atggtcttgt ccggttcgcc gatggcaaga
     2341 tgcagatctc caatggcaaa aagggacata ttccctccct tctccgaata aagcgggaat
     2401 ttacgcagaa actactcccc gtaatccaac aagggaggat aaaaacatga acgaaaaaac
     2461 gaactgcaag atcgactgct gcgtaaaaga ctgcaagtat cacaccgcgg cggaccagtg
     2521 caccgcttct cacatcaacg tttccaacga ggacgcccag cgcaaggcag agacgttctg
     2581 cgccacgttt gagaaccgct gcaattgctg accggacggg gctttcgccc cgtacatatt
     2641 tttttaatcc agcgtgaatg cgttctccca gtggcggatg tcgggtttta tcgtgtccac
     2701 cccgtccggg gcgtatattt cgatcacatc gaaccgcggc tgcagcttct gcggattctt
     2761 ttgcagccat tcctccgcgg cggcggccag tttctgccgc ttggcaaagg tcacaaactc
     2821 acgcgcctcg gcaaatgcgg cgtttttgcg cagcttcacc tctgcaaaga tcacatatcg
     2881 ccggtcggcg gcgatcacat ccagctcgcc gaggcgggta cggtaattca tggcaagaat
     2941 gcggcagccc tttttccgca ggtattccgc cgcgagcgcc tcgccgtagc ggccgagcag
     3001 catcgtgccg ctcaatgcat cttcctcaga tacgtcgggc ggtggatcgg cgacgggccg
     3061 tattcacgaa gcgcggcata gtgcgccgcc gtgccgtagc ccttatgcct ggcaaagccg
     3121 tactggggat agagtgcgtc catatcatcc atataccggt ctcgcgtgac cttggcgagc
     3181 accgaggccg ccgcgatcga agcgcacagg ctgtcgccct tgactacctt gatcgccggg
     3241 atgtcaagct cgtttttatc atttccgtcc acgagcgcga gtatgggcgc aggctccata
     3301 tccgcaatgg cgcggttcat ggcgagatac gttgcgcctt taatattcag cgcttcgatc
     3361 tcttcgaccg tggcaaatgc gatgccgaac gccacggcgc tctcgcagat gatgtcgtag
     3421 agcgcgtcgc gctttttcgc cgtgagcttt ttcgagtcgt tcagccccgg gatcacgagc
     3481 ccctccggca gaaggaccgc cgcagcgcac accggcccgg ccagcgggcc gcgccccgcc
     3541 tcgtccacac cgcagaacgg cgtcttgccg tccgcgagaa aacgctcgta ggcatacata
     3601 tccatttcgg ggtgttccat cgtcacggcc tttccagcgt cattcgcccg agcttgccct
     3661 cgcggaattc atcgagcagg acgtgcgcca tgcgctcggt attgacttcg ccgccggaaa
     3721 tgagaaagcc gcgctttctc gccgcggctt cgagcagctc gtagccgggc gcttccgggt
     3781 cgggcgtaaa tttatagcgc gcctcgatcg cgccggggta aagctcgcgc agccgctcga
     3841 gcagccggca ggcgacaagc tccacgtcaa ggatgttatc gttgatcgcg cccgtccagg
     3901 caagcgaata cccgacctcg ggcgactcaa acttcggcca gaggatgccg ggcgtatcca
     3961 gcagaaggat gccgccgttg agcgtgatcc actgcttgcc gcgcgtgacg ccgg
//
LOCUS       DAWAAE010000165         3936 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_8965_length_3936_cov_2.601907, whole genome shotgun sequence.
ACCESSION   DAWAAE010000165 DAWAAE010000000
VERSION     DAWAAE010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8965_length_3936_cov_2.601907"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          756 a         1184 c         1242 g          754 t
ORIGIN      
        1 gtcgtgggct ccgccgtgac gtcgggcgtc ggctcgggcg tcgcggtcgc ttccggttcg
       61 gccggtacgt ccggacggtt ggcgcacgcg ccgaaaacga gcgcgcatac gagaagcaga
      121 gaaagtacgg tcagaaactt gcgcttcatg gtgtcacctc atagtagtaa aaagttgggg
      181 ccgaatatta aaaatatact atatccgtcc gtttgtgtca atgcacgccg gtcacgaata
      241 gctcgccccg ccgtaatact cggagacgaa cgcgatatac gttttgccga cgccgagctg
      301 cagctcgctg ccgtcggagg tatagtagcg gaacggctcg ttcacgctgc cgcggctcca
      361 ggtgatcggc tcggcatagc cgccattgaa gaagtaaccg gcgtcctgat agtcgtaggt
      421 gtaaatggca gagtggttct tctcgtcgat gccggtcgag gtgggggagt agaggacgag
      481 gacgttttca aatacgacgc tctcgccggt atttccgtcc gtgtaggcgc tgttccactg
      541 cgtgctcgtg tagccgccct tttcggcgtc gtaggaaaag tcggtgatct tgtttgtcgg
      601 gaacacgacg cggatgctgc ccgccgcctc accgttcacc ggcgcggcat cttcggtgaa
      661 gtgcagaccg tagtcatagc cgtcggtacg gtgcgtggtg cgggtgccgt actgcgcaaa
      721 aagatcctgc aggcgctcgc cggtcgtcgt catgctgtgc acggcgccga agtacggcgc
      781 gtcgtactgc cggaagccgc agctgccctc gtggacctcg atatcgtcgg cgtcggtgta
      841 gtttacgagc atcgtgtcgg caaattccag accctcgccg ctcttgccgc agtgggcgaa
      901 aatggcgtca tagctgagcg cggcgctcac aaaatagctg cgcgcgctgc ggatggggaa
      961 gacgttgttc agcccgtccg tatccatgaa gaggaacatg ttgcgcgtga tggactccac
     1021 ctgcatctcg tagacgatgt ccgcctgcga aagaccgccc tgcgcgatga gcgtgccgtc
     1081 gtcccagtgg ttgttctcca ccatgatggc gacggggcgt gtctggctgt agtcggtgta
     1141 cgtctgctca ccggtcagcg ggttcgtgaa catcggctcg ggcgtcgcgg tcggcgtggg
     1201 ttcgggcgtg gccgtcggct ccggcgttgc ggtaacggca ggggtagaag tcacctccgg
     1261 cgcggccgag ggggcgggtt tggggcacgt gccgcagcct gcggtcagca gcagaagcgt
     1321 gcaggagagc agaagcgcga tcgttttgga aaatttcata ggagtgcacc tcggtgttca
     1381 ataatagaaa tatacgacca taggacgcat ggagcggccg tttggttcat atgaaaaagc
     1441 ctcggaagcg gtgcttccga ggcttttgaa aagtcgggga tcagtgcagc agattcagca
     1501 ccagcgtgag aacgacgaac gcgagcgcga cccacttggt catcttcgcc agagtaccgt
     1561 caagcgtttt cgccttaccc ttggagaaga aggtctccgc gccgccggcg acagcgccgg
     1621 acagaccgga gctttttcca gactggagca gcacgacgac gatcaggaag ataccggcga
     1681 ggagctggat gatggtgaaa gcaagaacaa gaccgttcac gagattcaat cctttctatt
     1741 ctaaaagaaa acttttttgc gcttagatat gttaccacag ccacagccaa aaagcaagct
     1801 atttttcgcc cccttccggc tgaaatgcag ggaacatccc cgcggcttga ctttgccctg
     1861 ccgccagaat atactttata aaaaaagaca cggaaatccc gacaaacgac cggaggaaca
     1921 gagcctatgt gtacctgcct tatcgccggc cgccgcgcct cgcgcagcgg ctatgcgctg
     1981 ctcgccgcca acgacgactg ggacgatacg cccgcccttc tcacgcacgt tccgcggcag
     2041 aagcatgcgc cggacgctgt atatacgctc gtcggcggcc atacgatccc cgagatcggc
     2101 gagacctgcg gctatcttta taccgcctgc aaatacgaga tcggcacgct cgaccgcgcg
     2161 tgggccggcg gcacgaacga ccggaacgtg tccgttgccg gtacaggcgt gatggcgttc
     2221 aaagcgatcc catgggaggg catggtgctc gagccggacg atatcccgct tctgatcctc
     2281 cgccgcgcgg agacggcccg cggcgccgtg gagctcatcg gcgagctcgt cgcgcgctac
     2341 ggcctgcgcc cgtcggggct cgattcctgc cagagcgggg cgacgttttc catcgccgac
     2401 ccgtccgagg gctgggtact ggagatcacg ccgggcggcc gctgggtcgc ggtgcgtgtg
     2461 ccggacgacg aggtgtcggt gcgcgcgaac gccttcggca cgcacgacgc tgatctgacc
     2521 gacacggcga acgtccttgc ctccgagggt cttgcggact atgcccgcgc gcagggctgg
     2581 tggaacggcg acgagcgtca tttcgacttt gcctgcgcct acggcgcgga caaaagcccc
     2641 aacgagtggg gcccggagct cgactctatg aacatgcgcc gccgctggcg cgccatggag
     2701 cttctctccg gccggccgca ggacgagaac gaaacgctct acagcgtccg accggcggaa
     2761 cccatcacgc gccggacgct catggacgtc ctcagcgacg tgtacgacgg cacgaaatac
     2821 gatctcacaa agctcccctc cggcgacccg ttcgcggatg tgacgccgga ctacgcgctc
     2881 tgccgccgcg gtacgctctc gagcattgtg gcggactatg cgccggagac cggcagcgtc
     2941 ctctggacgg cgatcgccac gccgcgcatg agcttttata tgccgctcta tgtcgatatt
     3001 gacggtcttc ccgccttctg cgaggcattc ctgcccggag agggggagaa cacctcgctc
     3061 ttctgggagt ttaaggagct gtgcattctg acacagcggc gctttgccag acatattatg
     3121 gagatcacgg ggaaagcaaa gcgcgagtgg gaagcgcatg ccgcggcggt ccttgccgcc
     3181 cacagccggg atatggcgca gcttccggag agcgagcgcc gcgccgcgcg cacaaagttt
     3241 gaccgcgccg tccttacctc cgcgctggaa acctgccgcg agacgcggca tgccctgctt
     3301 ctgcaatatt gatataggaa aagcgcccga aggatcaaaa atccatcggg cgctctctat
     3361 gttttcttat ttcatcgcgg cggcaaactt ctcttccata aacttccgcg cgttcgtgat
     3421 ctcggcgcgc cagtcctggc cgggctcata ccacatttcg atcatgtacg gcccgcggta
     3481 gccctcgcgc tcgagctggc cgagacacgc ggggaaatcc acgcacccac tgccgaaggg
     3541 gacgcatttg aacttgccgg ggaaatcctc cgtcacggcg agcgtatcct tgagatggac
     3601 accgaccatg ccgtttcgtc cgagtgatat ctccgcggcg gtgtcgttgc cccaggcgga
     3661 gaggttgccg acatcagggt agaccgtgaa ccacggcgag cggaggacgg ccttgtattt
     3721 taagtagcgg gagatgctgc tcatgagcgg cgtatccatg atctccatgg cgagcatgac
     3781 ctgatacttt tcggcgatct cgcacgcgct gcgcagcccc tcttcaaaaa gccgcaggct
     3841 ctccggcgtg gagggctcat agtacacgtc gtacccggcg agctggatga cgcggatgcc
     3901 gaactcccgc gcgaacagca cggccttttc cgtgat
//
LOCUS       DAWAAE010000166         3918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9010_length_3918_cov_2.859436, whole genome shotgun sequence.
ACCESSION   DAWAAE010000166 DAWAAE010000000
VERSION     DAWAAE010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9010_length_3918_cov_2.859436"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          885 a         1107 c         1053 g          873 t
ORIGIN      
        1 gtacagtccg gccgggtcaa gtccgttgcc ggagaccata ccgctttttt ccagatcgct
       61 gatcatttga aacccgtggc tgctgccgcg ctgcagaacg accagtatgg caggctgaag
      121 aaatttatcg agataggagc cccgacaggc acacttttcc aaacacgcat ccataaaatg
      181 acgccccttt tccgcatgta tttgttccgt taaaatcata gcgcaaacaa cgcaggaaag
      241 tcaatatgcc tcgctcgtgt gctgcactgc gggttggccc ggctagagaa tctttgcgga
      301 tttttcctaa tattgtccaa aatctttcta ttttatcaat tactgcatat gttatacttt
      361 gtccgaaaat gaaacagaaa catcaatgta tattaacgct tcggcagcat ctatttgtct
      421 gtgttaacta gctcagacga tccggcgtta aaagacggag atacacacgg ttttattttt
      481 aattcatgca aaggagaaca gatcatgact cccaaagaat tactgctggc aacgcttaag
      541 tgtaagccga cgccgcgccc cgcatggatc ccgtttgccg gcgcttatgc ctcccgtctg
      601 aaaggctata ccgcgacgga gatgttccag agcgccgata agctgacgga atgccttctc
      661 gaggtcaacc gcatgttcaa gccggacggc gagtgctgca ttttcgatct tcagatcgaa
      721 gcggaagtcc ttggctgcga cctgatgtgg gcggatgacg gccctcccag cgttgcatcc
      781 catcctctgg ctaccgaaat gggagatact cccgttgtcc cctgcgaatg cacgctcccc
      841 ggcccgaacg acggacgcat cccgctcgtg tgcgaagcca tgcgccgtgt caagaaagaa
      901 atcggcgata ccaccgctct gtacggcctg atcaccggtc cgttcacgct ggcatcgcat
      961 ctgcgcggca ccgacatttt cctcgacatg cttctggacg accagtttgt ccatgacctg
     1021 ctcgactact gctacaaggc cgcgtgcaag atggcggaat acttcatcga cgccggtatg
     1081 gacgttatcg ctgttgttga cccgctgatt tctcagatct ccaccgagca ctttgaagag
     1141 ttctgctccg agccgttcac gaagctcttc gaccacattc gtgaaaaggg cgcttactcc
     1201 tcgttcttcg tgtgcggcga tgccacgaga aacatcgaag tcatgtgcaa gaccggctgc
     1261 gactccattt cggtcgacga gaacgtcaac ttcaagcccg caaaagagat ctgcgacaag
     1321 tacagcgtag ctttcggcgg caacatcccg cttacgacca tcatgcttca tggcacgcag
     1381 atggacaaca tgaagtactg cgttgacctg atcgacagca ttgagaacaa gaacggtctc
     1441 atcgttgcca ccggctgcga tatgccgtac ggcgtaccgt ttgaaaacac gatcggctgc
     1501 atgcaggcag tgctgcagac ggacgaggtc cgcgaaatgg tcaaggacta tgttgcggaa
     1561 gacgatagcg acatcgaagt tgagctgccc gactatgagc atctcaccaa gcccctggtg
     1621 gaagccttta cgcttgaccc cgccacctgc gccgcctgca cctacatggt cgcggccacg
     1681 gatgaggcga agcggacttt cggcgatgcc atcgactata acgtttataa gtataccgtc
     1741 aaggaagaca ttgcccgcat gaaaaaaatg ggcgttccca atctgccgtc cgtttacatc
     1801 aacggcaaga tgaagttccg ttccattatc ccctccaagg acgaactgga ggctgcgatc
     1861 cgcgaagtca tgtaaccaag cgcggccgtc cggagcgttt tcgctccgga cggtcactcc
     1921 gcggtctgtg cttgtttttt ttacatattt agtagaccac gtccaatata agacgagacc
     1981 aatatgaacg aatttcgagg tgataaacat gccgatgctt cctgatccgg cgcatatgga
     2041 catcatcgat aaggcgtgca tttctgcctt tgaggcggaa gagccggtca gcgtattttc
     2101 gctttgcgaa aatattatgg acgatccgaa gctccccatg cattgcccgt atcaccatta
     2161 tctgatccct gccgtattgc tcacggccgc cagcaaaata agcggtgaaa cggaggaaat
     2221 gctgcagaaa cagctcaaaa cggcgcggga gcgcgccgga acggtgccgg ggggctgctg
     2281 cggacagttc ggctgctgcg gcgccgctgt gggcgcggga atttttgcct gcgtttggca
     2341 gggatcaacg ccgctctcca aaaccggctg ggcggcaggg aatgagatga ccgcccgctg
     2401 cctctctgcg atcgcttcgg tggaaggccc ccgctgctgt aagagagtga cgtatcttac
     2461 actctcggcg gtgcttcccg cagcaaggga gtttctcggt ctcgatctcg gcgagatacc
     2521 ggagcacacc tgccatcatt tcagccaaag ccgggaatgc cgtggaaccg cctgcccgtt
     2581 cttccccaaa aagaaaaaag cggaataatt aacggaggat aaaaccatga aaaaaaccat
     2641 tgcactgctg ctcgctgcca ttttgctcct gtctctcgct gcctgcggac agaccgcgca
     2701 gcccgccgaa cccgcggcaa ccgaagttcc tgcggaagct cctgccgagg tggaatcgtc
     2761 tgcggaaccg gataattcca atgttaatta ttctctgcgc gttgcgcaga tgggcaccag
     2821 cattaaggcg gccatggtcg ttcttgccaa cgaaatgggc tactatgccg aggaaggtct
     2881 tgatgtcacg tttgagccga tcaacaacct gaacgacggc atcaccgcca ttctgggcgg
     2941 cagtctcgac attcttccgt tcggcgtgat ccccacctgc accttcgttg cgcagggcgc
     3001 cgatctcacc gtcatcggcg gcacgatcgc cgagggcagc gagtgcgtta tgacgccgga
     3061 aaatgccgcg acctataccg acggcgattt gacctggttt gccggcaaga ccgtcgcctg
     3121 cgtccgtccg gaaaccggcc atatgatcat gatgcagaag atcgcgcagg ccggtgttga
     3181 tatgacgact gtgaagtttg tggagcttga cggcttccag tctgttgttg aagctgtgct
     3241 caagggcgag gcggacgttg gcttcgtgaa cagcggcttc ggccagaacg ccaagacgca
     3301 gggcctcgaa gtgccgttcc tcgtcggtga atatgcgccg aacgctgtct gctgccgcca
     3361 gactgcgctc aagagcaatg tggaagcgaa ccgtgacgcc tatgtccgct ttgagatcgc
     3421 caatctgcgc gccatgatgc tcatgctgac cgacaaggag acgaccattg ccaaactggt
     3481 cgagttctcc ggtcttgatg agcagtatgt ttacaactgc atttacgacg gcgttatgaa
     3541 gatctccatg gacccctccg ccaaccgcgt gcaggagttc tatgagatca tgcaggcaaa
     3601 cggcgatatt gccgccgatt ccgagtacga catgaccgac catgtagatt ccgccattta
     3661 ttacgatgct ttgactaccg ttatggaacg ttatcccgat ttcgacgggt ggacggccat
     3721 gatggaggcc tacgaagaaa ataacctctg atatgggcgg cattgaagtc aaagatctgg
     3781 ttttcactta ccccgggacg tcttctgaga cgctccgggg cgtggatctc tctgtcggcg
     3841 agggagaatt cctttccctc atcgggccct cgggctgcgg aaagtccccg ctacttcggc
     3901 tgctcgccgg attgcaac
//
LOCUS       DAWAAE010000167         3896 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9074_length_3896_cov_3.275449, whole genome shotgun sequence.
ACCESSION   DAWAAE010000167 DAWAAE010000000
VERSION     DAWAAE010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9074_length_3896_cov_3.275449"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          844 a         1174 c         1200 g          678 t
ORIGIN      
        1 caagtacgcc aacgagcagc tgcccgccgc caaggcgaac gcggacgaaa ttctgcagca
       61 ggccgaggcc tacaaggaat cccgcatcgc cgaggccgag ggtcaggcct cgcgcttcac
      121 ggagctgtac gccgaatact ccaagtatcc ggagatcacg cgccagcgcc tgttctatga
      181 aatgatcgcc aaggtgttcc cggatatgaa gatcgttatc accggcaggg acggcagcac
      241 gctgcagacc gttctcccgc tcgagagctt caccggcacg aacacaacga ccacgacagg
      301 ggaggagccg taattatgga aaataataac acccgccgtc ccgcccacgt cggccggacg
      361 atccttatcg cggtgctcgc cgttgtgatc ctcgtgctgc ttttgagcag cttgtacgtc
      421 gtacagccga acgaatacgg cgtcgtgcgt cagttcggcg cggtcgttga tgtaaagagc
      481 gagccgggac tgtatttcaa gatccccttt gtgcaggaga tgagcacgct ccccaatacg
      541 gtgctgctct atgacttgcc cgtgagcgat atgatctccg cggataagac gacccttatt
      601 gccgactgct ttgccatctg gcgcatcgag gacccgcgcc tcttcatcga aacgctctcc
      661 ggcagcgtat ccaatgccga ggggcgcatc aacgcgaacg tgtataacgc gctcaaaacc
      721 gttctctcgt ccatgacgca ggcggagatc atctccgggc gcgacggcac gctcgtgcgc
      781 accgttatgg cgacgatcgg cgattccttc gaccgctacg gcattgagct catcgccgtg
      841 gagacgaaga agatcgacct tccggacgat aacaaatccg ccgtgttcag ccgcatgatc
      901 tccgagcgca acaacattgc cgcgggctat cttgccgagg gcgagagccg agccaaggag
      961 atccgcaacg aggccgacaa gcaggtgcag atcctcctcg ccaacgccga cgcgaccgcc
     1021 gccacggtgc gcgccgaggg cgacgcggag tatatgcgca ttctctccga ggtgtacgac
     1081 tcgccggagg aagccgagtt ctatacgttt atgatcgcgc tcgacgcgct gcaggcgtcc
     1141 atgaccggcg cggaaaagac gctgatcctc gacgccgaca gcccgatcgc gaagctcttc
     1201 tattaagaaa ttgaacattc tctatctcat cagctacgcc ggaaaggcgg gtacggaaaa
     1261 gtacgtcgca aacctgatgc ggacgtattc cacgcaggga caccgctgct tccttgccta
     1321 catggacggc ggagagctcg cggaaaaggc aaaggctctc ggctgcggcg tgttccggct
     1381 cgacctgcgc gccgcgcacg cgcttgccgc ggcgaagagc attgcccggc tgtgcgcgga
     1441 aaacggcatc gacgttatcc acgcgcagta cccgcgggaa aatgtgatcg ccgtgctctc
     1501 gcgcctctgg cggcgggaga cgcgcgtcgt gttcacgagc catcttacgg tgcgccagag
     1561 cttcctctgg cgtgccgtca accggcgtat cacaccgcac gatgccgcgt gcatcgcggt
     1621 ttgtacgcag ggggcgaacc ttctgcgcga aaacggcgtc aaagaagaga gggttcgcgt
     1681 gatcttcaac ggcgttgcgc cgaagcccct gccgccgcgg cgaaacgtca tccggacgga
     1741 gttcacgctg ccggaggatt gcttcgtttt tctcaccatg gcgcgctatg cgccggaaaa
     1801 ggggcttttc ttcctgctgg acgcgcttgc gcagctcaag gagcgcacgg acgctccgtt
     1861 tgcttgtctc atcgcgggcg acggagagct ttttgacgtc gtgggcgagg gcatccgtga
     1921 gaggggcctt gacgaaaacg tcatccgcgc gggctaccgc acggacgccg aagcgctgat
     1981 gtgttcctcg gatgtctacg tgagcagcgc gctgtataac gaggccatga gcttcgccgc
     2041 gctcgaagcg atggaatgcg gtctgccgct cgttatgacg gacgtcggcg cgggcgcgga
     2101 cctcgctgac ggctgcgggg aatgcgtgcc gcccggcgac gcgagcgctc tgtgcgatgc
     2161 gctgcaccgg ctcatgacgg acgggatgta ccgccgcgcc tgcggcgaaa acgcacgccg
     2221 ccgcgccgtg acggagtttg acctcacgcg ccagacggaa gagctgctcg cgctttatca
     2281 aaaagggtaa accagccgcg ctgcggctga aaaaaggtat aaaaaagaaa agagtgattg
     2341 caaacgatga aacgcaccgt aatgaccgtg ctgctgtcgg tgatccttgt catcggcgtc
     2401 gtgtgcggtg tctactttct gaagcaggcg gcggctctcc gtgccgacgg cgagagcgcc
     2461 gttgcctcgg cgcaggagac gctcgccgcg gcacagacgc ggcacgacgc cgttgatccc
     2521 gagaccgaga ccggtgccgc cgcatggacg gcggcggagc aggaggtctt tggcgaaacg
     2581 caggaaaaga tcgacgcgct caaagccgac aacgccgcgc tcgatgaatc gatcacggca
     2641 atggaaaagg aagtcgaaac gaagctcgcg gacgaggaca cagcgtacta caattccgtc
     2701 tacgaggaaa tgcagaaggg agtggatcag gttgaagaat accttaaagg taattaacta
     2761 tgttctgctt ggcctcacgc tcgtcgcggt gggattcggc gtgtttgcct ttttccagta
     2821 tcgcgccgtg ccgatcgcgg aatacgaatc cagcgccgcc gaccttcgcg cgcagacgca
     2881 gctcgtcgat gaaagcaccg acaagctcag cgaggacatg aaggcgcagc aggacgcgct
     2941 gcgcgaggac atttcgcagg gcggcgacga gggcaagacg ctcgcttccg agcttgccgc
     3001 ggcgctctcc gataacgagg ataagaccgc gcagcttgaa gagcttcgcg ccgagaacga
     3061 gcggctcgat aacatcgtgg aagagacgct tgccaagcgg cgcgagtatg cttccaagat
     3121 ccgcgagctg gaggaaaagg tgcaggccgg cgaaacagat atcaagatct gctactggac
     3181 atttgacgac ggccccacgc gcctcaccac gcaggtgctc gacaaagcca aggagctggg
     3241 cgtccatgta acgttcttca cctcgcacga ggcgaacgaa tccgaggacg agcccgaaat
     3301 gcttcgccgc gagtttgccg aaggccacgc ggtcgccaac catacatact cccaccagtt
     3361 cgctgccatg ggcaatctct accagaagac cgagggtctg atcgaaatga tccaaaaaca
     3421 ggacgagtgg gtgtacgaat gcaccggctt ccatacggag atcttccgct accccggcgg
     3481 ctcggcgtgg gccaaagcgc tgctcggcac cgacccgacc gaggctgtca aggaagcggg
     3541 ctacgagtgg atcgaatggt cgtgcgacgt gttcgataac ggcgtcgccg gaaagaccgc
     3601 cgatgagatc cagagcatgg ccgtctggca ggtaaagcag ctcgacattg ctgtcctgct
     3661 cagccacgac tggaacgcca acacgctcat cgcgttcccg ggcgctgtca agcagctgca
     3721 ggacgcgggc tatgtgttcc tgccgctcta tcccgaatcg gtcaccatgg gcgagaatac
     3781 aaagatcaaa ttcagctgat atacgcataa cgcagacaag cggcgtgacg gtttccgtca
     3841 cgccgcttga gcttgtcaaa aatagtattt ttgacaagct ctcaaacgag acccac
//
LOCUS       DAWAAE010000168         3880 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9115_length_3880_cov_2.975425, whole genome shotgun sequence.
ACCESSION   DAWAAE010000168 DAWAAE010000000
VERSION     DAWAAE010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9115_length_3880_cov_2.975425"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          941 a         1033 c         1003 g          903 t
ORIGIN      
        1 ggcttcgcgt tccgctggta ctttgtcggc ggcgggccgc tgtacgagga tatccggcgg
       61 cggcgcgacg cgctcggtct cggcccgtgc gtgacgctga ccggatatat ggaaaacccg
      121 tgtcccctgc tcgcgcggtg cgacgcgctc gtgctctttt cggagtatga gggcacgccc
      181 gtcacgatcg acgaggccaa aacgctcggc gtgccggtca tcgccaacga cgtcggcgga
      241 gtaagggaga tgctcgaaga cgggcggtac ggcaagatta caagcgacga cagcttcatc
      301 aaacttatca ctcagctata gaaggagatc cagggatggc gaaaattatt tttacggtcg
      361 acaacataaa ctacaaaggc ggcggacatt tcgccacgtt taagatcgcc aactatcttt
      421 gcaactgcgg ccaccgggtc attttgtact ctcccgttaa agcggaagca tcggtgcgcg
      481 cggagcttgc tgacggaatc gtggtctcac aaagagcctc tttttccgat gccgactata
      541 tcgtcgttcc gtttgagaat tcaaccttct ttgaaaaaat cgcaaacctc aaaacccgcg
      601 caaagaagat ccagtggata cacatcgatt acgacgtctg gaagaacgtc gtgcaggacg
      661 atacggagcg cagaaggcgg ctgctcacag cctatgacag gatcgttttt gtttcggaac
      721 ataaccggaa caactttttg aagtatttcc ccgaacacgc agaaaaaagc acggtagtat
      781 acaatttcgt agattctgac aaaatccgcg ctatggcagc cgatgcagtc gatgcggaac
      841 ttttctctaa gaaaacgtcc aattcgctga ccgttgttct gcccggacgg ctggaagagc
      901 agaaagcctt tcaccggatg ctggacgccg ccaaagtact gaaagagcgt ggattgaata
      961 ttgaatggct gattttagga cgcggatatg aatacgattc ccttctgcag aagaaggagc
     1021 gctacggtct ggacaatgtg cattttctcg gctttcggca aaacccgtat tcctacatga
     1081 aggccgccga cgtaacggcg attttatcgg aatacgaagg tctcgctctc accgtagcag
     1141 agagccttac cgccggaacg ccggtgctgt ccacccgcac cggcggtgtg gcggaactgc
     1201 ttccggacga atacggctgg attatagaaa atgacctttt gtcgattata gatggaatga
     1261 ccgccattta cgatgaccgg aaattgctgg aagaaaagag aactgctctt cgctcctatg
     1321 cgtacaacaa cgaacgaatc aaagaaagtc tggacgccct tttccagacc tccgaagaaa
     1381 gaggccgcgc cgtgatgcac acgcaaacta tctactcctc taaaacgccg gatatttccg
     1441 tcattatccc ggtatacaac accgctgact atctcccgga gtgccttgac tctgtggtgg
     1501 gacagacttt cgatagcttt gagatcatta tcgttaatga cggctcgaca gacaagtcgc
     1561 agacgatcat cgatgactat gtatatcagt tttctgaccg tatccgggcg ttcaccatac
     1621 cgaacggcgg gctgggcaac gcgcgcaatt acggtatcgg caaagcccgc ggaaaatatc
     1681 tcgcattcgt tgattccgac gattttatcc actgcgatat gctcaaaaaa atgtatgaag
     1741 ccgcacggca gcacaatgcc gactgcgtga tggcggatta tatcgccttc tgggacgacg
     1801 ggcgggaaga gctcgtccgc tccgttgagt ttcccgacgc cggacggccg gatattatga
     1861 aatactccgc caagtacggc acagtgaata tctgcacaaa gctggtcgcg cgcgagcttt
     1921 tcgatatcat ccgcttccct gccggattct acgaggatct cgccacaacg ccgatccttc
     1981 tgagctgggc caaaaacgtc tcctatctcc gcgaagggct ctatttctac cgccagcgcg
     2041 tcggttccat cacgtcgata aaatccggcg acaagcgcct gctcgactgc tatgccgcgt
     2101 gggatcgtat ccgcgaacac gccaaccctc tctttgaaaa ggaaatacag tttgcggtat
     2161 actggagcct caactttttc tgcaccaatt ttctcgatga cttcacaaag cagtccaagg
     2221 attactatga ccggaaccgg gactatttcc gcggaaacgc ttatattgcc gacgcgatcc
     2281 gtgaagagac atttctggat tttgagcatc tggacacgat cccgaaaatc atccattact
     2341 gctggttcgg caacggggag aaaagcgagc tcattcaaaa atgcatggat tcctggaaga
     2401 agtacgcccc ggattttgag atcatggagt ggaatgaatc caattgcgac attcacacaa
     2461 accggtatgt ggaagaagcg tacgaaaaga agcagtatgc ctttgtttcc gattattttc
     2521 ggttaaaggc gctgtacgat cacggtggcg tttacatgga tacagacatg gaacttcatc
     2581 agccgctgga aagctttctc tatgcgaaat cgttctttgc ttttgaaacg cctctcttca
     2641 ttcacgccgg tattttgggt gccgaaaaaa actgcgggct cattggtgaa ctgcgccgct
     2701 cgtatgaaga agacactttc gatctgacag agtgtcccgg agaggacttc acgatcccac
     2761 gccgtcttac gcagcttctc atcaaacgga cgaacctgca attgaacgga aaatcacagc
     2821 ttttggaagg caacatccgt gtattcagcg ccaatcgtat gactgtgaat atgcacgacg
     2881 ggagatgtgt ctgcgagcac cattacgagg gcagttggct aagaaaggat aatggtccag
     2941 ctccggatta tacatacgaa gttttgaagc attacttcac atgggatctg ctgcacggag
     3001 acaacgacat atctctcccc ggtgatacgg cgcagcttct aacatactac aaaagcgaat
     3061 gcgatcgcta cgaaaactcc acctgctgga agatcaccaa gccgctccgc attctcggag
     3121 attttctgaa aaagattttc cggcgcaaca aagtctcctg accgggacat attaccgggg
     3181 tacaggttat gaaaaaaaac aaaaactatc tcgcattgcc ggccatcgtt ctggccgcgc
     3241 tctatcttac ctttctgatt gacctccata cgctcgaaac ctcattgcag agagtcttta
     3301 tcttatgcta ttttgtggtc atgggcgtgc tggcatgctt tttgaagggc aaatacgccg
     3361 agggaaagcc gttcccgctc caaagccgga tcgccgcggc gctgctcgcc gcgctgctcg
     3421 taggcgcggg ctggaacacg ttcctgccgc atgtgcgctt cccgctggaa ggctcgcaca
     3481 tgtacgcgct ttgggaaaag ctgctctata cgctcggcgc gtggtgcgtt gtgttctact
     3541 ttgcgcagct cgcgctgctg tttctcgccc gccccatgga aaaacgcggc tggacagagc
     3601 ggcagaaagt gctcgccttt cttctcatgg gaaactttgt gtttctcttt ttcacgagcg
     3661 atcgcatcca gccgacgcat ctgacgcagg gctttctcct gctgctgacg gtcgcttccg
     3721 tctggtgctt caaccggaag agcggctgta tggaaaagta ccgcacaaag ggatcccgtg
     3781 ccgccattgg gataatcgcc gtttatgcct cgcttacgag cttcgcgcag cgcttctttc
     3841 tggacggcaa cacgaggatg cacttttcgc tgccggggct
//
LOCUS       DAWAAE010000169         3783 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9376_length_3783_cov_3.441524, whole genome shotgun sequence.
ACCESSION   DAWAAE010000169 DAWAAE010000000
VERSION     DAWAAE010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9376_length_3783_cov_3.441524"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          808 a         1155 c         1134 g          686 t
ORIGIN      
        1 tagtcatcga gctctgcgct gccgctcgta tagcggccgt ttttatcgaa atacaggccg
       61 tcaaattccg aatcggtgag aaaatacccg tcgtcgccga gccggtagag atatccgccg
      121 aaccaaagga agccgtcgcg cgtcatggat tcaagagact ctttcgtgag cgttccgctg
      181 cccgccgcgg cgagcacttc ggcggatgcc cagtgcgtcg gggcgacgtc cggataatag
      241 acgtcctccg cggcgctctc tttcccgagc agccgggaaa cgcagaaggc gaattccgcg
      301 cgtgtcacat attcgctctc cgtttccggc agagcctcaa gcaccggttc aacggtctcg
      361 ggatcaaaaa gcgtgtccag cgcggtcttt agctcctcgc gcgtgagagc atcctcggga
      421 gcggcatcca gacctgcaag cagtgacgga tcgatacgct cctccggcgc gagcgcgcgc
      481 agcgccgcgg cgcactccgc gtgcgtcatc gcgctctccg ggcggagcag accgtattcg
      541 tcggggaaga gaaacgccgc gccggcgttg agcaccggcc gcgccagagc gtaatatacc
      601 gtgtccgttt cgaccggcgc tgccggatcg gcgcgcctgc cggtctcatc cgtccatgcg
      661 acgagataca gaccctcggg cagcgcggcg tccgcagcgg gcgagccgtc gaacgggatg
      721 ctctcggttt ttaaaacgtc gtcgccgaga cggtattcca ccgtgaaaac cggcgtttcc
      781 gtcggcgcgg gcgtggtctc cgccgcagcg ggggcgtttg cgatggggac aaccgccgcc
      841 cggtagccgc agccgtggag aaaaacggcg gcgagcagca cggcggcgag cggcgcgaaa
      901 cggagtgttt tcagcttcat gaagacggtt cctcccggaa ttgctttttt acagaacatg
      961 cgatacaatt ataatattcc cgccgcggcg tgtcaaatac gctcgcatcg cggcggcaac
     1021 agcgaggaat gtatgagaaa aaaagcgaat aaagagagat tgcccctgct tttcgccgcg
     1081 ctgctcggcg cgctcctatt gtcaaactgc ggctatcgcc cggagggggt cgaagccgtg
     1141 cagccgcttt cggacgtgcc cgccgccgcg gcggcgctgc cggaggagac cgccgcgccg
     1201 caggggaaga cctataccgt gacgtaccgt gtgaacggcg cggaaacgac cgagcttgtg
     1261 gcagagggcg gcagtgtgca gaatgcgccg gagtttatgg caagcgagag ctgcgccatc
     1321 gtcgcctgga aaaacgccaa cggcacaaag gtggacatcc gcaccgcgcc ggtatatgcc
     1381 gacgcggtgt atgaagccgt ccccggcccg gcgctccggc gcgaaggggc gtatatcgcc
     1441 gcgggcaacg acggcctttt ccacccgctc gataaattca cgcgcagcga cgccgcccgc
     1501 gccgtatatg cgctgctcga tacgaagccg accggcgaga cgtttttgaa ggacgtcacg
     1561 acccacgcca agtgctacac cgccgcaacg acgtttgtga cggcgggcta tatgacactc
     1621 aacgaggggc ggttttaccc ggacgttgcc atcacccgcg ccgatctcac ggcgctgctc
     1681 gaaaaggtgt tctctcccgc ggcggtggag cgcgctcttg ccggtatgac ggacgaaacg
     1741 cccttcaccc gcgccgaggc tgccgcggcg atcaacgcgc tgctcgagct ggacacggcg
     1801 gggcttgcca acgcgccgta cttcccggat gtggccccga gcatggaaaa ctatgccgcc
     1861 gtggagcttg ccgggcagag cgggatgatc tcctggctca ccggcgaaag ggcggagccg
     1921 ggctttctca atctggacgg atacctctac tgcgtcggcg aggacggcta tttcctgcgc
     1981 gacacgatgg tcggcacgct gtactttgac atctccggcc ggtatacgag cggggacgat
     2041 gtgctcgata cgttcgttgc cgatattgtc gacgcaaaca cgaatgcctc catgacccgc
     2101 gaggaaatgc tgcgcgcggt gtacgagtat gtgcgcgatc actatctcta tctcaaacgc
     2161 agtctctacg aggtcggcga gaccggctgg gagatccccg aggcgctgct catgttccag
     2221 agcggcaagg gcaactgcta caacttcacc gccgcgttct ggtcgctcgc gcgcggcatc
     2281 gggtttgacg cggtgtgcta cagcggtctc gtcggcgtcg ggcgcgaccc gcacagctgg
     2341 gtggagatca cattcgacgg cgtcccgtat gtgttcgatg tggagaccga aatgtcctac
     2401 cgtcttgtga acgactacat aacaagcatg tactggaaga cttacgagga ggtcgcggcg
     2461 tggagctacg tccgcacccc ggaggaagcc gccgccaccg cgccggaaac cgcgggctga
     2521 cggggcttgc cgccgcatcc aggaaaaaat ataccggccg tctgccggaa tgaacgaagc
     2581 cctaacggaa aaaattccgt tagggcttta attattatac aaatgttggg aataagcatt
     2641 ctgcacaaat tataatataa aaattgaaag catttttctt gaccaaacaa aagcgatatg
     2701 ataaatttaa ttcatatagg gagaaaaatc acagacaacg cgccccctaa aacaaagtaa
     2761 agaggtgtat tgcgtgaaag accttaagca tctgatctat tttgagaatc tgcttcagga
     2821 tgcacacaac gagcttgtcg cacaggcagt acaagaggga gactatgcgc tgggctacag
     2881 ctgctactac atcccggaag ttctgctcaa ccttcccggc tgcttttctt cccggctccg
     2941 cgcgccgcgc tgcacatcca cggatgtcgc cagctattac atgaccaacc gcacctgccc
     3001 ctatgcccgc tcccttctcg agcgcgccat tgaaggcggg tacaactatc tcaacgccct
     3061 tctcggcgcg gaatgctgcg cgaccatgga gcgcatggag gagcatttct tcctcatcaa
     3121 cccggtgaag aatgagaagt tcttcgtcac gcagatcgac ccgcccatga agggcgacga
     3181 gaccaatctc aattactaca aggcgcagct caagctcaag gtcgtggaca agctgcacga
     3241 gaaatacggc atcgacacct ccgaggaggc catgcgccgc gccattgatg accataacga
     3301 gatcagccgc atcatcaccg agatcggcaa tttccgcaag gcggataacc ccaacatcac
     3361 cggctacgag ttccacgtta tccagctcgt cagccaggtg tgcccgcacc ggctcatcct
     3421 gccgtatctg gccgagacgc tcgaagagct gaagacgcgc gagccggagc cgaaattccc
     3481 gttccgcgcg agagttgtcc tcatcggctc ggagatcgac gatccggaat tcacaaagct
     3541 catcgagacc tgcggcgcca tggtcgtggc cgaccgttac tgcttcggct ccttccccgg
     3601 ccgcgaacag atcgagatcc gtgaggggga gaccgccttc gatgcgatct gccgccacta
     3661 tctgcactgg aaccagtgcg cgcgcttcat ggacggcatg aagatcgacc agcgccacag
     3721 cgaggtcaaa aagctcgcgg acgaattcaa ggcggacggc gtgatctatg agaacatgaa
     3781 gtt
//
LOCUS       DAWAAE010000170         3777 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9400_length_3777_cov_3.233208, whole genome shotgun sequence.
ACCESSION   DAWAAE010000170 DAWAAE010000000
VERSION     DAWAAE010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9400_length_3777_cov_3.233208"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          785 a         1082 c         1156 g          754 t
ORIGIN      
        1 attttcttat ttcaggtttt ccactttttg cagcggttgg cacggcggcg ccggtctctc
       61 atgccgcggg ccgtgagata cacggcggcg agcagcaccg ttgcgctgat gccgatgatg
      121 ggcgtataat ccgtttcgcc ctcggtcttg acctcaagcc agagcgaatc catgagatgc
      181 gtcgttttct ggtcaagata gaggaatgct tcgccgcggc tgagcgtttc ctcgctcggg
      241 taggcgatgt cggacgccgc cgtctccgga tccatatact ccttggccgc cgattccggc
      301 gtggaatagc caagatagtc aagattttcg ccgttcactt ccggcgagca gaggaagttg
      361 atgtaagcct ccgcaccttc cttgttcgtt gcgcacgtgg ggatgcacag cgcgtcgatg
      421 aagaagttga agccctcctc cgggaagcag aagcgaagat cctcgttttc cccgaccatc
      481 ataagatagt cgccagcgta gtacggcgcg atccacgcct cgccgcgctc catcttgtca
      541 tagatctggt ccatgacgta ggcctgcacg agcggcttct gctcgatgag cttgtaggcc
      601 gcggcgcgca gctcgtcctc gtcacgcgtg ttgggcgaat acccgagcat cgtttccgcg
      661 atggcgaaag cgtcgcgcgg attatcgaac atcaaaatct tcccggcgta tttgctgttc
      721 cagagaagat cccagctgcc gacgtcctct tcgtccacat aggcgctgtt gtagatgatg
      781 ccgacggtgc cccaggtata cggaacggag tagaggttat cggggtcgta ggcggtattt
      841 ttgtacgcct cgtccatgta ctgatagttc gggatgttgt caaagttaag cggcagaagc
      901 agtccctcgt cgatcagccg accgaccata tagtcggacg ggatgatgac atcgtaggaa
      961 gaaccgccgg tcttgagctt ggtatacatc gtttcgttcg agtcgaacgt catgtaattc
     1021 acatggatgc ccgtcgcctc ctcgaaggcg gcgttgatat cgatgtagcc gtccgtgccg
     1081 tcggaaatgt actggcccca gttgtagacg ttgatcgtgt tgggagcggc gtgcgcctcc
     1141 ggcgcgggga tcagcaccgc gcatacgagc agcgcgagca caagagagaa cacacgcttt
     1201 ttcatgcgct cgcccccttc ttcccgaaca caccgcgcgc ctgacggcgg cggcgctcat
     1261 cgccggcctg cacgagattc atcacgacca tcacaaggag gatcgcaagg aagagcagcg
     1321 tgaacagcgc gtagatcttc ggctggagcg gctttttcgt atagttgtag atctcgaccg
     1381 gcagcgtgac aaagctgggg ccgtagacga agtacgaaat gacaaagtcg tccagactca
     1441 tcgtgaacgc catgagcgcg ccggagagaa tgcccggcat gatctcatgg atgatgacct
     1501 tgaagaacgc ctgcacgggc gtgcagccga gatcgagcgc ggcgtcgagc aggtttgggt
     1561 cgagctgtcc gagcttcggc atcacggaca gaatgacata cggcaaattg aacgtgatgt
     1621 gcgcgatgag aagcgtcgta aagccgagaa cgttgttgag cttcatcatc tgcccggcga
     1681 acgcgaagag cagcgcgagc gagacgcccg tgacgatctc cgggttcgtg agcgggatat
     1741 tcgtcacggc catcgtgatg cggcgcatcc ggccggacat ggcgcggatg ccgatggcgg
     1801 ccatggtgcc gagcaccgtc gccacgagcg aggcgatcac cgcgacgatg acggagtttg
     1861 ccagcagcgg cagcagcacg ccgtcacgga agagcgcgcc gtactgcgcg aacgtgaagc
     1921 ccttccatac ggcgatatcc gtaccggcgt tgaacgacgc aacggccagc acgatcatcg
     1981 ggatatacag aaacaggaac acgatcacgg tgaaaacacg gttgaaggtc ttcatatcgt
     2041 tgcgacctcc tcttcgtccg agaagcggtt catgactgcg atgcatacga gcaccatcac
     2101 catcagcacg agcgagagcg ccgcgccgag gttcgggttg taggcggcct tgaactggct
     2161 ttcgatgacg tcgccgatga gcgtcgtacc ggcggagccc attttctgcg aaatgtagaa
     2221 cgtggacacg gcgggaacga acaccatcgt gaagccggag atgatccccg gcacgctcag
     2281 cgggagcgtc acctgcccga acacctgcgc cggggtgcag ccgagatcct cggcggcctc
     2341 ggtaaggcgc ggatcgagct ttacaagtac cgtatagagc ggcatgatca tgtacggcag
     2401 atagttatac accatgccga gcacgacagc gccgtctgtg cggatgagcg gcagcggccg
     2461 gaggccaagg cttgtgacga cgccgttgat gatgccggta tcgtcgagca gcgccaccca
     2521 cgcgagcgtg cgcagcagga agctcatgca catcggcagc atgacgagca tatagagaaa
     2581 acgctgccgc tccggcgcgg cggacgccag acagtacgcc accggatagc cgatcacaag
     2641 gcagatgagc gacgagagga tcgccagcca gatgctcttg agaaaaatgg ggagatacgt
     2701 ccccatgcgg gaaatgttat caaacgtaaa tgcgccggtg atggaatcgg taaacgaaaa
     2761 ccagatcacg atcccgagcg ggacgaccgt gaagatcgcc atccacacga gatacggcgc
     2821 cgcgagcgct ttattcttca tcggcggatt cctcccggct ctccgaggat tcctcctccg
     2881 cctccggatc gtattccgcg tcggacagct cgtcatattc ctccgagaag gagctgtagt
     2941 cgccgtagag tccggaatac ttgctcttgt gcatgatgtg gaagccgtcc ggcacgatgc
     3001 ggatgccgat gcgctgcccg acttccgggc tgtcggtcgt ctggatgagc cacttgaagc
     3061 ccttgaagtc tacgataaca tcgtactgca tgcccttgaa ggtgatgctc gtcacggtgc
     3121 cggtcagcat gccgtccttg acggggacga tatcgatatc ctccgggcgg acgacgacgt
     3181 ccaccggctc gtcctttgca aagcccttgt ccatgcactg gaagcggcgg ccgaacgttt
     3241 cgaccacata gtcctcacgc atgatgccgt cgagaatgtt gctctcgccg atgaagtccg
     3301 ccacgaaggc gttcttcggt tcattatata tatcctccgg cgtgccgatc tgctggatga
     3361 tgccctcgtt catgacgaca acggtgtcag acatggcgag cgcctcttcc tgatcgtggg
     3421 tcacgtagat gaaggtgatc tccatctgct gctggatgcg cttgagctcc tgctgcatgt
     3481 ccttgcgcag acgcagatcg agcgcgccga gcggctcgtc aagcagcagc accttcgggc
     3541 ggttgaccag tgcgcgggcg atcgccacgc gctgctgctg accgccggaa agggaagtga
     3601 ccttccggtt ctgaaagccc ttgagggaca cgatctccag catctccgtc acgcgctcgt
     3661 cgatctcctt ttcgctcagc ttggcaatgc gcaggccgaa ggcgatgttg tcatagacgt
     3721 cgagatgcgg gaacagggcg tagcgctgga aaaccgtgtt gatcttacgt tggtagg
//
LOCUS       DAWAAE010000171         3776 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9405_length_3776_cov_3.002956, whole genome shotgun sequence.
ACCESSION   DAWAAE010000171 DAWAAE010000000
VERSION     DAWAAE010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_3776_cov_3.002956"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          772 a         1074 c         1128 g          802 t
ORIGIN      
        1 gtggatctct ccgtcggcga gggagaattc ctttcgctca tcgggccctc gggctgcgga
       61 aagtccacgc tccttcggct gctcgccgga ttggaacggc cggaaagcgg cagtcttttc
      121 tgcggcggca ttccgataac cggccccggc agcgaccgcg gcgttgtctt ccagagcccc
      181 gccctgtttc catggatgac ggcgcggaat aatgtatcct ttgccatccg aaaaagcgac
      241 cattcgctct ccagggcgga agcgcgctcc cgcgctgagg aggcgctgtg ccgtgttggg
      301 cttgccgatt ccatgagcat gctgccaaac cagctttccg gcggcatgca gcagcgcgtt
      361 gctattgcaa gagtgctcgg tatgggctgt cctacgctgc tgttggacga gccgttttct
      421 gcgctggacc ccaaaaaccg cttcgctctc caggagcttt tgctcaagct ctgggaagag
      481 gacagaaaaa cggtcctgtt cgtgacacat gatatggatg aggcgatcct gcttggcgac
      541 cgcgtagcat ttatggagcc gggacggatc gtcttccggc acaagatccc gttttcacgc
      601 ccgcgccagc gggaaacgct gcttatttcc agcgagtgct gcgcgctgcg gcgggatatg
      661 atctcgctct tctataagtg gggcgaggcg gcggaggtga acctatgacg aagcgcgaaa
      721 caaaaagccg cccgattctc gccgttcaca ttctgttcgc cgcgctgatg ctctctatgt
      781 atctgccggg aaacgctaag gtcaaggcaa gccccgtgtg tatgaccgtg gcgtgcgttc
      841 tcatcgagct tgccgtgatc tatcagctga ttcgtcggaa aaaagcgcgt accgccggag
      901 atatcggcgc gatcgtcttt tttgttctgc tgttctggca ggtgtataca acgcgccttg
      961 accgcggaca tatcatcctc gtgccgacgc cggaagccgt tttctcggtc ttctatacca
     1021 tgcgcgagcg aatgatcctt ggcgtattct cctcgctctc gctgcttctt atcggcttcg
     1081 gcgtgtcgct gccgctcggc gtggctctcg gactgatcgc cggttggaac tctcgtctgc
     1141 gcgaaaccgt cgtaccgatc gcacgcgtgc tgtcgccaat accggccatc atctatgcgc
     1201 cgtatcttat cgcgatcatg ccgtcgttcc gctcggcttc cgccatggtt ttgatcatcg
     1261 gtattttctg gccgacgttt ctgcagatgg cagcgcgcgt cggcgcgctg gatcagcgca
     1321 ttctcgacag cgccagagta ttgggtctga aacagcgaga gctgttatgg gaggtgctcc
     1381 tgccgtgggt catgcccggc atactcaccg gtctgcgcgg ctcgctctcc tcggccttta
     1441 tgctgctgac gctcgctgaa atgatggggg cgcacagcgg tctcggctat ttcatccgta
     1501 actttgccga ttacgctaat tataccaacg ttcttgcggg catcctgctc gtggcgctgg
     1561 tcatcacgtt gctcaaccgc gcggtaacgg cgcttgaaac acgtcttgtg cggtggcact
     1621 gaaaagggga ctgttatcat gacaaagctg tatcttatca ccggatttct cggggcgggg
     1681 aagacgacgt tcctgcacca gtttgtacgt ctgtttccgg accggcgcac agccatcgtc
     1741 gtcaacgagt tcggcaagca gggcgtagac ggaacgctgc tggaggatct cgatatcaag
     1801 atggaagaga tcaacaacgg ctccattttc tgcgcctgta agatcgaaca gtttgaggac
     1861 gcgcttctcc gtctgcagaa ggagaatttt gacgtgatct ttgtggaagc ctccggtctg
     1921 tccgacccga ccgccgtcgg taccatcctc cggcagcggg ataaatttcc cgatttggaa
     1981 tacgcgggag cgatctgcct tgtggacggc gcacggttta tgaccgttta taagaccgct
     2041 cgcgtctgcc gtatgcagct tgccatcagc aatatgatca tcatcaacaa gtgcgacatt
     2101 gcctcccgcg agcagctgga tgagatcaat accatcgtcc gcgcgcagaa gccgaatcgc
     2161 ccgacctttg agacaacgtt tggccggatc cgaccggaat ggctgacggc gatggaagaa
     2221 cagcccgccg aggaaacgta tcggatcaag ggctttgcaa gtacgccgga gggacggttt
     2281 ctggttgact gcgtcggccc catggtcgag ctcagggaat ttgaaaatgc tccggccggg
     2341 gaggagaata tgcttgtcgt cctctttggc aacgggcttc cggcacagcg cgcaatcaag
     2401 gaagccgtca gctggttccc aaacgcggaa gtgactatag aataaaaacg gcctccgata
     2461 aacacacacc ccgcctgccg aacggtagat accgtcggac aggcggggtg ttgaattatt
     2521 ttatgaaagc aggagacttc gcagcgctgc aacgctgtct gcgatggcgt cggttccttc
     2581 ggcttccagc tcttcgcggc tgccgtagcc gtaaagcacg ccgatgaaat cgatccgcgc
     2641 catttttgcg ccgatagcgt cgtgtgaccg gtctccgacc atgacgcagc cggagcgatc
     2701 ggaaatgccc atttcgtaca gcgcgtgcag gatcacctcg cacttatccg gcggctcgcg
     2761 gtcgagcgga atgccggaaa caacgtcgat ataccggcgg agaccaaagt gttcgaggat
     2821 ctgctcggag aaaaccgtcg gctttccggt tgcaacggca atgcggacgc cggacgcctt
     2881 tagatcgcgc agcagctccg gaatgcccgg ataaacctcg ttttcaaaaa gaccgacggt
     2941 cgaaaagcgc tcgcggtaat agccgagaag ctcctgcgcc tcctccggtg tcgcgttcgc
     3001 ataaacctca aacgaacgga gcaggggagg gccgatgtac ttatggtagc tctcgatcgg
     3061 gccgaccggg cggccggcct tttccagcgc atagcggacg ctgccgcaga tgccctgcac
     3121 cggatcggtc agcgttccgt ccagatcgaa gagcacggtg gagtacttca ttcagcgctc
     3181 ctcccggaag tacgaaaggc cgagacttgc cgggggatcg ttctcccgtt ttttctgcat
     3241 ggaaacgatg accagaacaa ggatcgtgat cacgtagggg agcattttgt aaagctcctt
     3301 caccgcgaga ttcatgccgg acgggatgaa catatggaga atgtacagtc cgccgaagag
     3361 gaacgaagcg aaaacgccga tgttcggacg ccacacggtg aagataacga gcgcaatagc
     3421 gagccacccg cggtcaccga agccgttatt ggaccatacg ccgcaggcat agtccatcac
     3481 gtaatagagt ccgccgagac cggcgatcat gctgccggcg caggtggcga catacttata
     3541 ttttgtgacg ttgatgcccg ccgcgtccgc cgtgttgggg ttttcgccca cggcacgaag
     3601 ctggaggccg atgcgcgtgc ggcgcaggat atacgcgcag ataaacgcaa taataacggc
     3661 ggcgtaggca aggaaaccgt aggagaggaa aatcttgcca aaccagccga gcttgccgga
     3721 aaacgggagc gatgcgctga aaaaggcgct cgtacgcgaa agggcaatgg agggga
//
LOCUS       DAWAAE010000172         3764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9439_length_3764_cov_2.918576, whole genome shotgun sequence.
ACCESSION   DAWAAE010000172 DAWAAE010000000
VERSION     DAWAAE010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9439_length_3764_cov_2.918576"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          729 a         1013 c         1026 g          996 t
ORIGIN      
        1 gcactgctcc tgctgttacg atggcaatgc ccgttttttt aggcattatt cttctttttc
       61 ttcaccagca tcgccccgcc gagagcaata agaacaagtc ccgcgccgcc cagcacccag
      121 atgggccagt tgagctgacc ggtggggatg aggggctttt cgttgttgaa gccctcgacc
      181 gtgaacaccc agtccacttc gccgacgcgg taggcgtact cgttgccgag cccggcggga
      241 accgtgagca ccgcttcgag cttcgcgctc ttgcccttgg cgaaggtgcc gagaaggacg
      301 ttatcggtga gctgcgcggt ctggtccggg gaagcggaga agatgctctg cttcgtgccg
      361 tccgcgttca cgagatagac ctccatcgtg agcttggaga ggaagtccgc catggtgacg
      421 acggtctcgt ctcgcattcc ctcgatgttg gcctgatcgt ggccgtcctc gttctcgaag
      481 gtctcgctgt aggtgagcgg gttgccctct tcgtcgtggg cctcggcgcg gatgtagagc
      541 ttcacatagt caaagccgct gacgttgttt ttgatcgtga tgttttcggt cagctcgtcg
      601 ccgggcatga cgtttttgaa gttgccaaaa aggtcggtgt ccatgtagcc gctgccggtg
      661 gtctcgatca cgagaccgtt ggtaccctga cggctcacca cggcgtcggc ggcgagagcc
      721 gggacggtca gcgccgcaag cgcggcgatg accatcacga gggagaggag cttcttcatc
      781 gattttttca tgtcgttact ccctccttag ttaccgctga caggcgttac gccccatgct
      841 tcctgaacgg ccttggccgg agtgctctgc accgccgtcg ccagaatttc cacagacagg
      901 atgtactccg gttcaactcc ctgcggatac gtcaccgtgc agttgacaag actgccgtcc
      961 gtgaggcctc cgggcgctac gggtgtcaga tagtagaaat atccgtccgt gccctttgtc
     1021 cacgctttgt tcggattctc actgattgag agaatgtagc cgtccggggt ggttccctgc
     1081 gggacggggg cgatgctccc gtccttgttg agccagctta ccacataggt ggcgcggatg
     1141 taggcgtccg tggttccggt gttttggatc tgaactttct cctttgtggt gccgttgaag
     1201 ctttcactga ccgtgcagga gacgctggcg taggtgaact tgttctccac cggcgctgtt
     1261 ttggtcacca gaaaggcgac ggtagagccg accaccgcgc cgatcagcat gacgaccgcg
     1321 atgaacagga tggccagctt gttcatgcgg attctcggtt tgttgttcgc tttcgcgtgg
     1381 gatccattat acatgattgc cgcctccttt ctcagttacc ggaatctgtt tcgatgatcg
     1441 ttccgaatac gttcttgctg taggcgtcgc cgccgagcca cttagtgatg ctgcgcgtgt
     1501 ttgcaaagtt aaaggtgtac acatcgtcgg gcttcgtcgg cgtgatgtcc gcagacttta
     1561 cgtagtcctt gttggtatag cgccagctcc agtcggtgat ctcggtaacg gtgtatgtac
     1621 cgaccttcag ccccgtgatc ttcacggagg tgttgccctt gatcgcgaca gtcgtgctga
     1681 agccctcgcc ttccaccttg aagaggaagc tctgattttc gtcgatgtcc tcacagcctg
     1741 tcttgctgat aatcagatcg gcaagatcat actcaaactt ggcgtagtac gtcgcggcct
     1801 cgtagccctt tacatcgttg ccaaggttgg cggtcttctg cggggtgagc ttgttgtccg
     1861 cgatccaagc cgcgtctacc ggctgcgtgc aatccttgtc cttataccag ccgacgaact
     1921 tgaatccggc gctcgcggta ggcgtggagc cttccggcgt accgtcgatc accttgaccg
     1981 tgttgtcctg atagttgtcc agcgtaccgc agccggtcgg gccgacgacc tcgtatttga
     2041 tgtcaacagt atcttcaacg taatagaaga ttttaacatt gttgaccgcg gtattgcctt
     2101 cttcggcaac gatatcgatc gtctgctggt ttgttgtacc gagaaccagc gtatagcccg
     2161 ggacttcttt cgccccctgc gtcacgcgcg cctgatagcg ggcgctgccg gttacaggat
     2221 cagcaacctt agtttccgtc gtcccctgaa caaggaagcg gaattcgtag ggatacacat
     2281 tgcggtcata gtagagcgta agtacaagac catccgccga aagtttgccg ctggctgtgc
     2341 tcttgcctgc gttatatgtg aaccctgtaa gtgtcagcgg gctttcggaa taatctgttc
     2401 cgatcgtagc attgaggttt gtgctgctct ggtactcggt gtactcgtcg ttggtgagat
     2461 tctggatcag gtggattttc tgcagcggtg catgcgtctc atctttcgta taccagaagg
     2521 tcagcacgtt ttttgtatca tcggcagaga gaaccaactg cttctgatac gcatccggcg
     2581 cataaccctt gatcttcacg aatttctccg tgatcacagc gtccgaagtc tcgctggact
     2641 tctctgcata gagaacagtc tccgtattcc gttccagata acgaaccgtg taatttactt
     2701 tttctctggc aacatatacg aacgtgtact cgttaccctc aatgtccatc tgaatggagt
     2761 ggctgccttc cagaggataa tagcctgtct gataaccttc attcagcatg ccgaacgttt
     2821 tcgccgcaaa cgtcttggtc gttccggcga gcgcagaccc tgtggtatcc tccgcgatgg
     2881 ttgttccatc ctcaagccgg tagtgaataa cgtagtctac attaacgtcc gctctccact
     2941 tagcgtagag attcaggtca cggttgaccg gcatggagaa gtcaaacgct ttttcctcgt
     3001 cggtatcgct gatgtagaac cagccaacga aggtgtaagg ctcacgctcc cgattcgccg
     3061 ggggatccgt taacgctgtg ccatgcggaa catccgtcca tgtctgaatc gcttggccgt
     3121 tattatccag aacatccttc gtcaggtacg ttttgaccgt gtgggtcttg ggcacccatt
     3181 tggcatagag caccagatcg gaggcgggca tggtcagcgt gttcagatct gcctcggaac
     3241 cctcaaagca accagacgtc gtgtaccaac cggagaatac atacgcgttc ttttcaagct
     3301 tatccggata atccggttta aagtaatagc tgctcaacgg agcttcgtac tgtacgctcc
     3361 cgcccttgcc agcaactact gcgttatagt tgtagaacga cagattgtag ctgttgcggg
     3421 tatagtacag gtagttattt ttctgcgcgc taacagagcc gtttactggc aagtagggat
     3481 tagattcacc ttgtatgaag ccgagaatag gatggaattc ttccttatat gtcaggttga
     3541 tattgttgcc cttgaggtct atcactttat ataaattata atttcttcca ttataggaag
     3601 tctgaccaga ggcccccttc agggtttcaa cataataata aagtttagcg ccagactgtg
     3661 attcatactt agtaaatgtg atgttctcac ccggcatggt atcaatgctg ccgaggtagt
     3721 tgtcgggctc aaaggactga cacccgctcg gcaccgtcca ccag
//
LOCUS       DAWAAE010000173         3761 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9442_length_3761_cov_3.104155, whole genome shotgun sequence.
ACCESSION   DAWAAE010000173 DAWAAE010000000
VERSION     DAWAAE010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9442_length_3761_cov_3.104155"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1980..1989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3408..3417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          727 a         1160 c         1062 g          792 t
ORIGIN      
        1 cccttgttaa tcgacgcccg agcgcgaaca attaaatgct tgcttaatcg ttataagcgc
       61 agtatactgg ctttgttccg aaaaaacaac ccgtttggac gttctgcttc caaacgggca
      121 aacgaaattt ccgaaaagga gaaccgccat gccggagttt tctctccccc acgaccacgg
      181 cacgcaccag cacgaggagg aggttcgccg cgtccttgcc gacgacgcgc gcttttccgc
      241 cgtggcggag ctgttccgct cgctcggtga tccgacgcgc gtgcgcattt tctggctgct
      301 cagccaccgg gaggagtgcg tcatcaacat tgccgcaatg ctcgacatgt ccagccccgc
      361 cgtatcgcac catctgcgct cgctcgccga gaacgggctt cttgagagcc ggcgtgacgg
      421 caaggaggtc tattaccgcg cgcccgatac cgtgcagggg cagctgctgc atgagatcgt
      481 agagcaggtg atggagatcg cctgcccgga aaagagcgta gaccggtgcg cgtcgcagga
      541 ggagatcatc cgaaacgtcc acaactacat ggtcgagcat ctctccgagc gtctcacgat
      601 cgaagcgctg tcgcgcgagt ttctgatgaa cacaacaacg ctcaaggccg ttttcaaaaa
      661 ggtgtacggc acgtctctcg ccgcgcacat gaaggcgcac cggatggagc gggcggcctc
      721 gctgctgctc aaaacgcagg acgacatcgg cgcgatcgcc cgcgccgtcg gctacgagag
      781 ccagagccgg ttcacctcag ccttccgcga atgctacggc gtatcgcccg gcgaataccg
      841 ccgcgcgcac acgccggctt gattttttcc ctcaccgaaa gcaattgaca ggccgcgaaa
      901 aaagatatat cattaaattt atataatctt tacatttacg ggtgagagta tgctttcgca
      961 ggagatcctt gatctttata aaaagtcacg cctgatgaca taccgggcga tgttcgggcg
     1021 catccgggag aagaccggct cgctctccgc tacggaggcc tacgccgtgg acgtgatcta
     1081 tctgctcggc agcccgacga tcacgcagct tgccgaaacg ctcggcatat cccagccgaa
     1141 cgccacctac aaggtcaaca accttgtggc gaaagggtac gcctgcaaaa cgatctcgga
     1201 ggacgacaag cgcgagtgcc gcgtcacggt cggcgagcgg ttttacaagt actacggcaa
     1261 ctccacgcgc tttatcgaca acgccgtgga cgcgctccgg gaggattatt ccgaagacga
     1321 gctgaatacc tttgaaaagg tcctgcacag tctgaaccgg cacatcgaaa aaagggagga
     1381 aaccaagcta tgacgaccgg aatgaatctc tgggacgccg acgtatggag ctttgtgctg
     1441 acgctctcgc tgctgtttgc ggcgatgatc gcggccaaca ttctccgccg gctctgtccc
     1501 tttatccgca aggcgctgat cccgagctcc gtgctcggcg gctttctgct gctgcttgcc
     1561 aacgccgttg cccgctgggg tttccgccac ccgctcttct ccaccggcac gctggaaatc
     1621 ctcacgtatc atggtctcgg tctcggcttt gccgcgctcg cgctgcgcaa taccgagaag
     1681 aaaaccgacg gccgctcccg caccggcgcg ttcgacaccg gcgtcacggt cgtgaacgga
     1741 tatcttctgc aggcggtgct cggactgatc gtgacgatcg ggctctatta cgccatacgc
     1801 agcttctacg cctccggtct gcttctcccg atgggctacg gccaggggcc ggggcaggcg
     1861 tacaactggg gccacaacta cgaaatgcag tggggctttg aaaacggcac gagctttggc
     1921 cttgccgtgg ccgcgatggg ctttgtgtcc gcaagcgtcg gcggcgtcat ttatctgaan
     1981 nnnnnnnnng gatcttctag ggacggctcg gcgaggacgc cagggacaac gtcacactct
     2041 cgaccttcac cggcgacaac gaggtgccga tctcggaatc gatggacaag ttcacgatcc
     2101 agcttgcgct cgtgtttctc gcctatgcgc tcgcgttcct ctttatgaag ggtataaact
     2161 cgctgctcga ccccgccggg acgggcgcga aggggcttgc gggaacggta cagggcatga
     2221 tctggggctt ccagttcctt ttcagcagcg ttttcggcat gctcatcaag gcggtgatga
     2281 agatgctccg caaaaagggc gtcatgcacc gcgagtatac aaacaccttc atgcagaacc
     2341 gcatcgcggg cttcatgttc gatctgatgg tcgtggcgtc tatcgcggcg atcgaccttt
     2401 ccgccttccg cgaccaccgg ttcgtcctgc cgctgacgat catcagcctc gtcggggcgt
     2461 tcggcacata cttctatctc cgctttgtgt gcgtgcgtgt gttcccgtgg tatgagcacg
     2521 aggcgttcct ctcgctttac ggaatgcaga ccggcacggc gtccacgggc gtcatcctcc
     2581 tgcgcgagat cgacccgcag tttgaaacgc aggcgagcga caaccttgtc taccacatgc
     2641 cgtgggcgat cctcttcggt gcgccgatgt tcctgctcgt cggcgtggcg ccgcagagcg
     2701 tcgggaagtc gtggatggtg ctcggcgtat gcgcggcgct gttcgtgatc ttcaatgtga
     2761 tcctcttccg ccggtcgatc ttccgccgca aggccgttac gaaataatac tctttgatac
     2821 atacgataat ggggcagcca ttcaaccgaa tggctgcccc gatttttata gctgatacgg
     2881 cccgtggccc tcctgccagc cgacaaagtc gctgacgatc tcgcgcatgg cggtgaagtc
     2941 cgcggcgcgc gccgctctct ggcggagctt ttcgcgctcc tgctcggtga gcgcgccgaa
     3001 cttttccagc gcaatgccgt tctccgcgag agcgcggtca aagtccgttt ttacggatcg
     3061 gttttcattc atgtgcattc cctccctgcg gagagtatgc cccggcgcaa ggcgtttatg
     3121 cgttctcgcc gctcatgtaa tcgtaataat ccgattcgct cgcaaagagc atgtattttc
     3181 cgctcaccag tccgtagtat ccgccgtggg tgtaatagcc gcgcatgtcg ttgccctccc
     3241 tgtcctgttt acaagacaag gatacggcac agagtgtacg ataaaacgga cttccgcgtg
     3301 tgaagaagga taaacctctc cgtcaaaacc tccggttttg ccacctcccc tgttagggga
     3361 ggcaagtttg ttatacgcaa acttacttgg ctcccctaat aggggagnnn nnnnnnncgc
     3421 gcttcctcca tggtctccgg tttcggttcc tccatcgctt cgatctcggc ttcctcacgt
     3481 tcccgctcgc gccgcttttc gccgcgcacc gcggcagtca ccgtgccgcc gagaatggcc
     3541 gcgcctccgg cgagcttcac aaatgccgcg gcggtcgaat atcctgccca gacaagcggc
     3601 agcacctcat cgagcgctgc gagcgccgcg gcgccgccga gaagccacca gaggtttttc
     3661 ttttttacga tatcccgcag ctttttctcc gggaagaacg ctttgacaaa ggcggcgaga
     3721 ccggcggcaa gggcaagcac cgtaatcccc cagccgagca c
//
LOCUS       DAWAAE010000174         3691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9642_length_3691_cov_2.754400, whole genome shotgun sequence.
ACCESSION   DAWAAE010000174 DAWAAE010000000
VERSION     DAWAAE010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9642_length_3691_cov_2.754400"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          777 a         1112 c         1073 g          729 t
ORIGIN      
        1 tcccaagaca aacttcaagc tgcgcgactg ggtgttctcc cgccagcgct actggggcga
       61 gcccatcccg atggtaaagt gcccgacgtg cggctgggta ccgctgccgg aggatcagct
      121 tccgctcctg ctcccgcagg tcgagagcta cgagccgaca gacgacggtg aatcgcccat
      181 ctccaagatc accgactggg tcaacacgac ctgcccgaag tgcggcggcg cggcccagcg
      241 cgagaccgac accatgccgc agtgggcggg ctcctcgtgg tatttcctgc gctatatgga
      301 tccccacaat aaagaggcgt tcgcaagtcc ggaagctctc aagtactggt ctcccgtgga
      361 ctggtacaac ggcggcatgg agcacacgac gctccatctc ctgtattccc gcttctggca
      421 caagttcctc tacgacgagg gccttgtccc gacgaaggag ccgtacgcca agcgcacgag
      481 ccacggcatg atcctcggcg agggcggcga aaagatgtcc aagtcccgcg gcaacgtcgt
      541 gaacccgaac gacatcgttg agcagtacgg cgcggatacg ctgcgcgtgt acatcatgtt
      601 catcggcgat ttcacgaaag ccgccgcatg gtcgagcaac gctgtggcgg gctgccgccg
      661 cttccttgac cgcgtgtgga atcttgcaag cgagccgatc accggcgacg agatctcccg
      721 cgacaacgag aaggagatcc accgcgccat ccagaaggtg agctctgata tcgacgagat
      781 gaagttcaac actgccattg cgcagctcat gtcgctcgtc aatcagttct actccaagaa
      841 accgacccgc ggcgatatcc gcgtgctgct gcagcttctc tctccgtttg cgccgcatat
      901 ctgcgaggag ctttggcaga tccagggctt tgagggcctt gcgagcgagg ccgagtggcc
      961 ggagcacgac gaggcgaaga tggtcgatac cgaaaagacc atcgccgtgc agatcaacgg
     1021 caagctccgc agcaccgttg ttgtcccggc ggacagcacg gtggaggtcg tttccgctgc
     1081 ggctctcgcg gaaaagaaga tcgcgggcta tatggagggc atggagatcg tcaagacgat
     1141 caacgtcccc aacaagctca tcaacttcat cctcaaaccg aagaaataat ttttaaaagc
     1201 aatgcgcccg aaaaaacttc gggcgcattg ctttttgtaa taaatccgga ataatacggc
     1261 atactaacgg cggatttctt gtttaggagg atttcgcatg aaaaagtggg tcgtatatat
     1321 tctttcgctc acggcgctgg ttgtttcgct ctgcgcgtgc ggcacggcgg aaaagcccgc
     1381 ggcgacgaac gcgccgaggc cgtcgggtac cccgatgacc gcgacgccgg acatgaacga
     1441 cgggatcgta aacgacgatg acggcatcat caccgacgac gataccggcg ttgtgcccac
     1501 gcagacgccg atggcggaca cgtcggccaa accgtccgcc tctgcgcagg cgacggatgg
     1561 cgcagcaaaa aagtgaaaaa caccccgccg gatccgaaat ggaccggcgg ggtgttttgt
     1621 aaagcgagaa agcctgtaag ccgggttctg tctgcgcagc catctatcta gacgcaccgt
     1681 tgccgatgcg ttccagccgc ctcctcggga cgaccgggcc ggtcgtttgt ccctccacgg
     1741 cgttgctccg gatagagttt acagcaccgt tccgtctccg gacggtgggt gagctcttac
     1801 ctcgcctttc cacccttacc tgcccgaagg caggcggtat atctctgttg cacttgtcct
     1861 ggggtcaccc ccggcgggcg ttacccgtta tccttgccct gtggagcccg gactttcctc
     1921 acgcacaggc tttcgcctta tgcccgcggc tgcttggctt tctcgcacag gtattttacc
     1981 tgttcaaagc gcgcttgtca acggcgatag gtgttgaatt tctgccggaa agcgaatata
     2041 atatataagt taaagaagat attcaggagg caattatgac actcgactct tatatacagt
     2101 ctctgcgcgg aaaaagcatt gcggtaatcg gtctcggcgt gagcaaccgg ccgctgctgc
     2161 ggcttctgct cgatgcgggg tataccgtca cggtgcgcga taaacgcacg cgcgaggcgt
     2221 tcggcgagga cgaggccgca gcgctggaag cggcgggctg ccgtctcgtg ctgggcgatg
     2281 ggtaccttgc cggtatcacc gaggacgtga ttttccgcac gccggggctt caccccttta
     2341 cgccggagct cgccgcggcc aaagcgcgcg gagcgcttct ttcgtcggaa atggaggcgt
     2401 tctttgccgt ctgcccgtgc cgcatcatcg ccgtgaccgg ctcggacgga aagacgacga
     2461 ctaccaccat tatttccgag ctcctgaagg cgcagggaca ccgcgtattt ctcggcggca
     2521 acatcggcac gccgctgctt gacaaagcgc cggaaatgac aagcacagac tgggcggtgc
     2581 tcgagctcag ctccttccag ctccacagca tgaactgccg gccggatatt gcggtcgtta
     2641 ccaacgtttc gccgaaccat ctggacattc atccgagcta tgaggactat cagaccgcca
     2701 aaaagcagat tttcctttca cagggctgtg ccggcgtcac ggtgctcaat gccgacaacg
     2761 ctatcacacg cgcctttgcg aaggattgcc ccggcatagt ccggtttttc agccgtgaga
     2821 ccgccccgga aaacggcgtg ttcctgcgcg aaggcgtcat ttaccggtct cacaacgggg
     2881 aaaagaccaa gctcatggac gcagaaagca ttctgctccc gggtttacat aacattgaaa
     2941 actatatggc ggcgtttgcc gccaccgacg gcatcgtctc ggacgatacc tgccgcgccg
     3001 tggcggagag cttccgcggc gttgcgcacc ggctcgaaat gatccggacg ctgcatggcg
     3061 ttacctactg caacgattcc attgcatcca gtcccacgcg cacgatcgcg ggacttcacg
     3121 cgctgcggca gaaacccatc cttattgcgg gcgggtacga taagcatatc ccgttcgaca
     3181 agctcggcga cgaggtctgc cgccatgtca aggcactgtt ccttaccggt tttaccgcgg
     3241 agaaaattta cgatgccgtg acaaaatcgc cgctttatga cccaaaaacg ctgccaatcc
     3301 ggaagatcga cgatttccgc gaggcggtgc ttgccgccgc cgcgagtgcg gaggaggggg
     3361 atctcgtgct cctttctccc gcttgcgcgt cgtttgacca tttcaaaaac tttgtggagc
     3421 gcggcgaaac tttccgctcc atcgttaacg aactgaaata aaggatacac tgccatgaat
     3481 tgcgaagagc tttatacact gtccgcccag gtggaggaac ggctccagcc cgtcttccgc
     3541 ggatttgagc gcacggcgga gaagaatacg aagcgcgtgc tggatgcctt ccgcacgcac
     3601 cgagtgtccg agccgtgctt tgccggaacg accggctatg gctacgatga tctcggacgc
     3661 gaaacgctgg agaaggtcta tgccgatgtg t
//
LOCUS       DAWAAE010000175         3673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9699_length_3673_cov_3.372582, whole genome shotgun sequence.
ACCESSION   DAWAAE010000175 DAWAAE010000000
VERSION     DAWAAE010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_3673_cov_3.372582"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          803 a         1097 c         1095 g          668 t
ORIGIN      
        1 cgatcttgta gccgatgttg ccggcctcga tgcaggggaa gatgaaggtg ttggcatagc
       61 ccgcgacctt ggagccgggg aacttgagct ggccgacggt gggggacacg gcggcgtcga
      121 actgcatctc gccgtcaatg gcgagctcgg gggccatttc cttggcgacc ttggtggcct
      181 cgtggctgag cgcgaccgtc gcgcccttgc cggagccgtt ggtggagaag gagagcatgg
      241 cgaccttcgg gtcgatgccg aagatcttgg cggtgcgggc cgtttcgatg gcgacctcgg
      301 cgagcttctg cgcggcggaa acggtcacgt tgccttcctt gtccacgctg tcctcgtagg
      361 agatgttgat ggcgcagtcg cccatggcga ggagctcttc gccgcgctgc agaatgaagc
      421 aggaggagac gagatgcgcg cccggacggg tcttgatgag ctgcagcgcg gggcggacgg
      481 tgtcggcggt ggagtaggta gcgccgccga gcagggagtc ggccacgccc atcttcacga
      541 gcatcgtgcc gaagtagttg cccttcttga gcagctcgcg gcactcttcg ggcgtcatct
      601 tgcccttgcg aagagcgacc atcgcatcga ccatctcgtc catcttatcg taggtcagcg
      661 ggtcgatgat ctccaggcct tcgatatcga agccgccctt ggcggccgcg gccttgacct
      721 cttccacgtt gccaacgagc accggggtga ggaagccgtc cttcttcagg cgggaagccg
      781 cctcaaggat acgggcatcg ggaccctcgg tgaacacgat gcggcgggga ttggctttca
      841 annnnnnnnn ngcataaata tactatcacg atttgcggaa aagtcaacct ttacaaacac
      901 ggaactctct tgttataata cagaaagtaa tcaacgcaaa ggagcgatcg ccccccatga
      961 caagagggtt ttcggagacc atgtccgagc tgcggcgcaa aaagggcgcc tcgcagcgca
     1021 cagcggcggc ggatctcggc atatcgcagg cgctgctctc gcactatgaa aacggcgcgc
     1081 gcgagccggg gctggacttt gtctgccggg cgtgcgagta ttacggcgtt tcggcggact
     1141 atctgctggg ccggacggac gagccgggcg gcgagacagc gccgcggctg cacgcgctgg
     1201 cggaggagct tcgcgctctt gccgcgcgcg tagagaacgc gcactgattc ttttttgagg
     1261 agtaccccta tgatcgacca aagccatatc cggaattttt ccatcatcgc gcacatcgac
     1321 cacggcaaat ccacgctgtc cgatcggctc attgagctgt gcggcgcggt ggaaaaacgc
     1381 gaaatgtccg agcagatcct cgacaacatg gatctcgagc gcgagcgcgg catcaccatc
     1441 aaggcgcgcg ccgtcggact gacgtacaag gccaaggacg gcgagacgta tacgctcaat
     1501 cttatcgaca cgccgggtca tgtggacttc aactacgagg tttcccgctc gctcgccgcg
     1561 tgcgagggcg cggtgctcgt tgtggacgcc tcgcagggcg tggaggcgca gacgctcgcc
     1621 aacacgtatc ttgcgctcga gcacgatctc gagatcctgc ccgtcatcaa caagatcgac
     1681 ctgcccgccg ccgacccgga gcgcaccaag accgagattg aagacatcat cggcatcccg
     1741 gcgatggacg cgccggagat ctccgccaaa aacggcatca acgtggaggc cgtgccggag
     1801 tatatcgtgc agcacgtccc cgccccgaag ggcgatgccg cctcgccgct caaggcgctc
     1861 atcttcgaca gccagtacga cgcttaccgc ggcgttatcg tgcttgtccg cgtcgtggac
     1921 ggcgtgatcc gcaaggatac gcccatccgc ctgatggcca cgggcgcgga gtataaggtc
     1981 ctcgaggtcg ggcatctgcg cccgatgggg ctggacccct gcgatgagct cgccgccggc
     2041 gacgtcggct acttcaccgc gagcatcaaa aacgtggagg acacccgcgt cggcgacacc
     2101 gtcaccgaga gcgcgcgccc ctgcgccgag gcgctgcccg gctaccgccc ggcgcgcagc
     2161 atggtctact gcggcattta caccgaggac ggctccaagt tccccgacct gcgcgacgcg
     2221 ctcgacaagc tcaagctcaa cgacgcctcc ctctcctacg agccggaaac gtccatcgcg
     2281 ctcggctacg gcttccgctg cggcttcctc ggcatgctgc acatggaggt cattcaggag
     2341 cggttggagc gcgagttcaa cctcgatctc gtcacgacgc tcccctcggt catctacgag
     2401 gtcaaaaaga ccgacggcac aacggtattc gtcgataacc cgcacaacta ccctgaccct
     2461 gcgtcgatcg ccgaggcact tgagccgttc gtcaaggtga cgatcatcac gccgaacgag
     2521 ttcgtcggca acatcatgcc gctctgccag gagcgccgcg gcgtttataa aaacatgcag
     2581 tatctcgacc agcggctcgt ggaaatgcat tacgaaatgc cgctcaacga gatcatctac
     2641 gacttcttcg acacgctcaa ggccaagacg aagggctacg cctcgctcga ctatgagctt
     2701 tcggagtacg tcccgtccga tctcgtgaag gtcgatatgc ttctgaacgg cgatcaggtg
     2761 gacgcgctga gcttcatcgc ccaccgcgaa aaagcctacg gccgcgcccg caagctgtgc
     2821 gaaaagctgc gcgacaacat cccccgccag ctcttcgaag ttcccgtaca ggcggccatc
     2881 ggcggcaaga tcatcgcgcg cgaaacggtg cgcgctatgc gcaaggacgt tctcgccaag
     2941 tgctacggcg gcgacatcac gcgaaagaag aagctgctcg aaaagcagaa ggaaggcaaa
     3001 aagaaaatgc gccagctcgg cacggtgcag atcccgacag aggccttcct cgcggtgctc
     3061 aagctggacg aataaaatcc aagcaggaat ttgagagaga aaaacggtta tggagttcat
     3121 gataaaaaca gaaaggcaca ccatacagcc gttttccgcc gcattcctgg caccatatta
     3181 caaagaattt actgccgaaa tcacaaaata ccaatatccg gacagttttc ccgatattca
     3241 tacggcggat atcgtcatgt ccaacttcgt caaggccatg gagcgcggag acatgttgga
     3301 gctggtcatt ctcacgcatg acagtgaatt tttaggaagt atggaagcgt ttgatatttc
     3361 cggaaaaacg ccggagctcg ggctatggct gaaaagctct gcgcacggga agggatatgg
     3421 ctacgaggcg ctgaaggggc tggtagacca cctgaacgcg accggccagt atctgtatta
     3481 catttacaac gtggatatcc gaaatgcagc aagcatccat ttggcggaaa aattcaacta
     3541 taaaaaaggc gaaaccgaaa caacaaccac agaatccgga aaagtactcc atacgcaaac
     3601 ctattatatt accggctaaa gctgcggcgg caaggtcagt tcagaccttg ccgccgtttt
     3661 gttatttcac gat
//
LOCUS       DAWAAE010000176         3626 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9832_length_3626_cov_3.077289, whole genome shotgun sequence.
ACCESSION   DAWAAE010000176 DAWAAE010000000
VERSION     DAWAAE010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_3626_cov_3.077289"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          754 a          992 c         1130 g          750 t
ORIGIN      
        1 gacgagcagc agtccgcaaa ggccgcatac cgcgccggag aggatcatgg tgcggatgat
       61 gaccttgtcc accttgatac cgacgtagcg cgcggtgcgt tcactctcgc cgacaacggt
      121 gatctcgtag ccgtgcttgg tgtaattcag atagaaatac atcaaaatcg tgacaacagc
      181 gacgatgaga atattcaaaa ggtatttgta gctgccgatc tgcgggaacc agccgatctc
      241 cgtggactgg ttgatgatgc cgatctggcc ggagcccttg gggttttccc agacgatgac
      301 gaaataggcc acgagctgcg ttgcaacgta gttcatcatc agcgtgaaaa gcgtttcatt
      361 cgtattgtat tttgccttga aatacgccgg aatgaagccc cagagcgcac cggccaggat
      421 gctgctcacg cacatgcaca tgataacaag ggcgttcggc agcgtgttgc gcaggcagat
      481 catgcacgcc gccgctgcga gcgcaccggc aagcacctga ccttcgccgc cgatgttcca
      541 gaagcgcatc ttaaaagccg gcgtcacggc gagcgcgata cagagaagca tcgcgatatt
      601 ctgcagcagc acccagacct tgcggggcga gccgaacgag ccgttaaaga tggtcgcata
      661 cacctgcagc ggattttcgc cggtcatcgt cgttgtaata atggagcata cgatgagcgc
      721 gagcaggatc gccgccgcgc ggatgcccca ggacttgtac caggggagag cgtcccgctt
      781 cgtaatgtgg aagagaggcg atcttgtttt cttattcatg gctctgtcct cccagcttag
      841 tcatcatcat gccgacctcg cgcttttccg cgccgcggcc ggagacgatg ccgctgacct
      901 tgccgccgca cagaacaagg atacggtcgc acagctccag cagcacgtcc agatcctcgc
      961 cgacgaagat aacggcgaca ccggctttct tctggcggtt gataaggtca tagatcgtat
     1021 acgacgaatg gatatccaga ccccgcacgg catacgccgt gagcagtacg gtcggcgcgc
     1081 ttgcgatctc gcggccgaca agcaccttct gcacgttgcc gccggagaga cggcgcacgg
     1141 gcgtatccac gctcggcgtg cttacgctca gctcctgcac cacggattct gccagtgcgc
     1201 gtggcgagcg gcggtcggta aagatcgatt tgccgcggcg gaaggaacgg agcatcatat
     1261 tgtcggcaag attcatgttg ccgacgagtc ccatgccgag acggtcctcc ggaacaaagg
     1321 aaagggagac gccgagctcg gcaatgctca ggggatcttt tccgatgagc tcgtcgcggg
     1381 aaccgtcatc ttcaatatag gaaatgctgc cggattcaac ggtctgcaaa ccggcgatcg
     1441 cctcgagaag ctccttctgg ccgcagccgg agataccggc gataccgagg atctcgccgc
     1501 tgttggcggt gaaggaaacg tcgtccagct tgacgatgcc gtccacgctg cgtaccgtga
     1561 gccccttgac ctcaatgcgg ggcttgggat cgaccgggtc cggacgctca atgttcagcg
     1621 acaccgcatg gccgaccatc atgttcgtca tttcctgcgc gttcgtgtgc gccgtgagca
     1681 tatcgccgat gaactggccg tggcggagga tcgccacgcg gtcggagagc gcctcgacct
     1741 catgcatctt gtgcgtgatg atgacgatcg cctttccgtc attgcgcatg ttgcgcagta
     1801 cggcgaagag cttgtcggtc tcctgcggtg tgagaaccgc ggtcggctcg tcaaggatga
     1861 gaatgtccgc gccgcggtag agaaccttta cgatctcgac cgtctgtttc tgggagacgg
     1921 acatatcgta aatcttctgc gcgggatcga cgctgaagcc gtatttgtcg cagatctcgc
     1981 ggatctttgc cgaggctgct ttgagatcga gcttgccggg cagaccgaga acgatatttt
     2041 ccgccgccgt cagcacatcc acgagcttga aatgctgatg gatcatgccg atgccgttgt
     2101 caaaggcgtc cttgggggag cggatgacga cttcctttcc gtcgatcttg atctcgccct
     2161 catccgggaa atagatgccg gagagcatat tcataagcgt cgtcttgccg ctgccgtttt
     2221 cgccgagcag cgcgaggatc tctccgcggt aaatattcag ccatacctta tcgttggcta
     2281 aaaccgggcc aaagcgcttg gtaatgttgc gcatttcaat tgctgcggtc tttgtttcca
     2341 ttctttcact ccctgacaga ttgtatggat ttcggcagac tgtactgtaa agggcagcgc
     2401 ggcggccgcg agctgctgta ctgcccttta cagtacatat ggggaaggcc cggaaatcgg
     2461 gccttcccct aagcaggacc agataaaaat caggcgtcga ggttggtgat gccgtcgatg
     2521 tcgagatcga agtacggagc ggagcggtag aaggactcga ggaacacgcc gtcggaaatg
     2581 gcctcggtct caccgacgta gtcaccgaga tcgtcaacgt cggcgagata ggtcgtaacg
     2641 gtctcgccgt tgacggtgaa ggtggcggtg tcgaacacct tgacggagcc gtcttccaga
     2701 gccttcatga cttcggcgat cttctcggca gtgccggcag cagcggcctg ctcgttgacg
     2761 tcagtcagaa cgacggagcc ggtagcaaga gtgccggtcc aatcgtagcc gatgtcctca
     2821 ccggaagcga cgcagttgat ggcgtagaca aagaacggag tccagtcaat gcgggaggaa
     2881 acgatgaagg tgttggggca ggcggcaacg gtgctgccgt tgtaggaaac gttggggaca
     2941 ccggcggtct cgcaggccgt gggagcgccc atggagtcgg cgtgctggct gataaggacg
     3001 cagccgttgt tgatgagctt gttggcggct tccttctcga gagcctcgtc ataccaggag
     3061 ctggtgaagg tcacttccat cgtgacgctg gggcagacgc tacgggcgcc gaggaagaag
     3121 gaggtgtagc cggacttgac ttcggcatag gggaacgcac cgacatagcc catcttggct
     3181 tcctcggcgg tgatgtcgcc gttttcgatc atctcgttga gcttgagacc ggcagcgaca
     3241 ccggcgaggt agcggccttc atagatggag gcaaaagcgt tgtggaagtt gctcagaccc
     3301 tcggtgtggg ccttgacacc ggtgcaggaa acaaagtgca cttcggggaa ctccttggca
     3361 gcggcaagag cgaaggactc atggccgaag ctgtcggtga tgatgagatc gcagccggcg
     3421 tcaaccagct cggcagcggc gtcatagcag gcctgggact cggagatgtt ggtcttcatc
     3481 atgacctgat cgtcggagag accgacctcg gcgcaagcgg ctttcgcagc gttgatgaag
     3541 ttgaggtcgt aggtggagtt ttcatcgtgc aggaagatga aaccgacctt gatgtccttg
     3601 gaaccggtag tggcttcggt ctcgct
//
LOCUS       DAWAAE010000177         3565 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_9999_length_3565_cov_3.341311, whole genome shotgun sequence.
ACCESSION   DAWAAE010000177 DAWAAE010000000
VERSION     DAWAAE010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_3565_cov_3.341311"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          708 a         1161 c         1120 g          576 t
ORIGIN      
        1 gtgcaagggc gttacggtcg tcggcggcgg cgacagcgcc agcgccatcg agaagatggg
       61 ctatgccgac aaggtgaccc acgtttccac cggcggcggc gcgtcgctcg aattcatgga
      121 aggcaaggag ctgcccggcg tggcctgcct gctcgacaag taatttttca ccaaaggggc
      181 gggttccgcc cctttgcgcg tattcaccga agggagaaaa ccgctatgaa caagaaatac
      241 agaaaagtca tcatcgccgg aaactggaaa atgaaccagc ttccctcggg cgtaaagccg
      301 ttcgtcgagg agctgaaaac gaagctgccc gcaggcatga acgggtgcag cgtcgttctc
      361 tgcgtcccgg cgacgcatct cgccgcgctc gcgcgcgaaa aggcgcggcg catcggcatc
      421 ggcgcgcaga acgtctccga gttcgataag ggcgcgcaca ccggcgagat cagcgtggac
      481 atgatcgccg atctcggcgc gaaatactgc atcgtcggcc acagcgagcg ccgcgcgaat
      541 aacggcgaca ccgacgagct cgtgaacacg aaggtccacg cgctgctcga aaagggcatc
      601 accccgatcg tgtgcgtcgg cgagtcgctc tccgagcgcg accgcgatct cacgatggag
      661 cgcatctcct atcaggtcaa ggcagcgctg tacggcctga ccgccgagca ggtgaagaag
      721 gtcgtcatcg cctacgagcc catctgggcc atcggcacgg gccgcaccgc gaccgacgag
      781 caggcgcagg aggtctgctt caacatccgc gagaatctgc gcgcgcagta cggcgcgctc
      841 gtctcccgca aggtgtgcat cctctacggc ggctccatga acgcgaaaaa ctgcgcgggt
      901 cttctcgccc agccggacat cgacggcggt ctcatcggcg gcgcgtcgct caagctccag
      961 gcctcgggtc tggacgtcgg tctgcccgcc ggacagatgg gcaacagcga ggtcggccac
     1021 acgaacatcg gcgcgggccg cgtcgtctat caggatctcc cccgcatctc caacgccgtt
     1081 gaggacggca gcttctttga aaatcccgcc tataagtccg ccatggacga gtgcgtgcgc
     1141 accggcgcgc ggctgcatct catggggctg ctctccgacg gcggcgtcca cagccacatc
     1201 aaccacctct tcgcgctgct cgaaatggcc aagcgccgcg gcgtgaagga ggtctacgtc
     1261 cacgccctgc tcgacgggcg cgatgttgct cccacctccg gcgcgggcta tgtgaaggcg
     1321 cttgtggaaa agtgcgccga gctcggcacc ggcaagatcg ccacgatcca gggccgcttt
     1381 tacggcatgg accgtgacaa gcgctgggac cgcgtggaaa agggctaccg cgccgtcgtc
     1441 tgcggcgagg gcgtgcagga tggcgatccc gtccacgccg tggaggagag ctacaaggcg
     1501 ggcgtgacgg acgagttcgt cgtgccgacc gtgtgcgata aaaacggctg cgtccgctcc
     1561 ggcgacagcg tcattttcat caacttccgc cccgaccgcg cccgcgagat gacccgcgcg
     1621 ctcgtcgatc ccgcgtttga cggctttgtg cgcccgaacg gcttcttccc gctgcactat
     1681 gtctgcacca cgcagtacga cgcgacgatc cccaatgtct ccgtcgcctt cccgccggag
     1741 gagatcgaaa tgacgttcgg cgaatatctc ggcaagcttg gaaaaacgca gctgcgcatc
     1801 gccgagaccg agaagtacgc ccatgtgacg ttcttcttca acggcggcac cgagacgtgc
     1861 taccccggcg aggaccgttg cctcatcccg tccccgaagg agttcccgac gtatgacctc
     1921 atcccggaga tgagcgccta taaggtagcg gacgagtgcg cgaagcgcat cgagagcggc
     1981 aagtacgacg ttgtgatctg caaccttgcc aactgcgaca tggtcggtca caccggcgtc
     2041 aaggccgcgg cgatcaaggc cgtggagacc gtggacgcgt gcgtcggaca gatcgtcgag
     2101 gccgtgaaga aaatgggcgg cgtgatcctc atcaccgccg accacggcaa cgccgaccgc
     2161 atcatgaagc cggacggcag ccccgacacc gcccacacca ccaaccccgt gccgctgatc
     2221 gtctccggcg cggacgtgaa gctccgtccg ggccgcctgt gcgatctctg cccgacgatg
     2281 ctcgatctca tgggtcttgc aaagcccgcg cagatgaccg gcgaaagcct gatcgtaaaa
     2341 taagtttccc gctctgcagg aaaggagttt tccatgaaag agtattttga aatcgttgac
     2401 gttctcggaa gagaaattct cgactcccgc ggcaatccca cggtcgaggt cgaggtgacg
     2461 ctcgataacg gcgtcgtggg ccgcgcggcg gtgccgtccg gcgcctccac cggcatttac
     2521 gaggcctgtg agctgcgcga cggggacaag tcccgctatc tcggcaaggg cgtgcagaag
     2581 gctgtggaaa acgtcaacgg cgagatcgcc gaggcgctca acggcctcaa cgcgctcgac
     2641 cagccgtata tcgacaagat cctcatcgag ctggacggca cgccgaacaa gacccgtctc
     2701 ggcgcaaacg cgatgctcgg cgtatcgctc gccgtggcga aggcgagcgc cgaagcgctc
     2761 gggctgccgc tctacagcta catcggcggt gtgaacgcca agacgctgcc cgtgccgatg
     2821 atgaatgtgc tcaatggcgg cgtccacgcg ccgtcctccg cggccgacat tcaggagttt
     2881 atgatcatgc ccgtcggcgc gaaaagctgg aaggaagcgc tgcgctggtg cagcgaggtg
     2941 ttccacacgc tctccaaggt gctgcacacc tccagcgtcg gcgacgaggg cggttacgcg
     3001 cccagcgatc tcaaaagcga cgaggacggc ctgcgcgcca tcgtccaggc catcgagctc
     3061 gcgggctatc ggcccggcga ggacttcatg ctcgccatgg acgcggcctg ctccgactgg
     3121 ttcaaagccg aggacggctg ctatcatctc ccgaagcgcg gcatcaccat gacgcggcag
     3181 gagatggtcg atatgtgggt ctcgttctgc gataagtacc cgatcatctc gctcgaggac
     3241 tgcatggcgg aggacgactg ggagggctgg aagatgctca ccgacgcgct cggccatcgc
     3301 gtgcagctcg tcggcgacga tcttttcgtc acgaacgtgc gccgcatcaa gatgggtctt
     3361 gaaaagcacg ttgcaaacag cgttctcatc aagctcaacc agatcggcac gctgaccgaa
     3421 acgctcgacg ctatagcgct tgcgaaccgc gcgggctaca ccacggtcgt ctcccaccgc
     3481 tccggcgaaa cggaggacac cacaatcgcc gatctcgtcg tcggtctcaa cgccggccag
     3541 atcaagaccg gcgcgccgtg ccgta
//
LOCUS       DAWAAE010000178         3526 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10114_length_3526_cov_3.001152, whole genome shotgun sequence.
ACCESSION   DAWAAE010000178 DAWAAE010000000
VERSION     DAWAAE010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10114_length_3526_cov_3.001152"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2521..2530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a         1081 c         1004 g          679 t
ORIGIN      
        1 aaaagcaccg aaaagactgg ctgtccgacg tgtgtgctct tgttaatcga cgcttgcatc
       61 atagcaaaaa atccgcccga cggcaatacg ctgtcgggcg gtcagctctt tagttttcga
      121 taatgcgctc cacgcgcggc gagagagcgc tcgtgagcgc gcacccctcg ttcgcgccgc
      181 agaagtttgc aacgtcctgc gagaggaggg cgttaccctc gcggtcaaag ccgaagagcg
      241 tgacttcctc tccgatctga acctccatgc cggtcacgtc caccatgcac tgatccatgc
      301 agcacgcgag catcggccgg cgctctccgc gcacgagaat gtcgccgtgc atctccacga
      361 gcgggagata gagcccgtcg ccgtagccga cgccgacaat ggcgacatcc gcgtcgtgat
      421 cgagcgtata caccccgccg tagccgagct tttcccccgc gtggcggcgg tggacggcca
      481 tgatacagct gcgccacgag gcacactcgc gcacattccc gcgcggctgc gtcggggggt
      541 ccatgatgag ccgccgcccg agacgcacgg cgtcaaagtg gtattcgggg taatactcgc
      601 tcccggcgct gtcgcagatg tggcgcatcg gcaccgcaaa gcccgcgcgc tcgacctgct
      661 cggcgctgcg cagcagaagc tccgcctgcg cgcggcagag ctccggcttt gcggtgtcgg
      721 caaaatggga gtagacgccc gcgagcttta tacgctctcc ggcggcggag agctctttta
      781 aaagcgcctc gagctcgtcc cccggtgtca cgccgacgcg gtggagaccg gtgtcgatct
      841 tgatttgtat tttcacttcc gtctgcgccg cttccgccgc cgcggcaagc gcgggaagca
      901 gaccgagccg tccggcggtg agcgtgagat gcgcgttcac ggcgtcggga acggcgtgcg
      961 gcagagggtt tccgagaatg agtgcgtcct tttcgatgcc gagcgcgcgc agcgccaaac
     1021 cctcggacac atgcgcgagc gcgaatgtgc ggatgcccgg cagcgccgcg agcgccggag
     1081 cggtgagcga aagagacagt ccataggcgt cgcacttgag caccgggatg atctccgcgc
     1141 cgggcgagag cgttttttcg atggcggcaa cgttcgcgca cagcgcgtcc gtatgcaccg
     1201 ccaaatagga attattcaag ctattcatat aataattcat aataccgcct ctgaattatt
     1261 cacatattca ccgttcttgt gcatatccaa actgccatat gcggcaggaa acgcccggaa
     1321 acgatgcata ttatattgca taaaagccat ttatgcaata ggaacctccg gcttttagca
     1381 atatcaccaa aaacagaggg agaaaaacgg aagaaatcga attaaaaacc attgacattg
     1441 tataaatata cgattatact atgcccaacc tgaaacaaat tcattcccgt tgaatggaaa
     1501 ggaaagaaaa gaaatgaaaa aggtaacagc aatcgttctg gctctgctca tggtgctgag
     1561 cctcgctgcc tgcggcagcg ctgcgtccga aagcaaaact ctcgtcatgg cgacctccgc
     1621 cgactatccc ccgtacgagt tcatcgttct gaacgacaag aacgagcagc agtacgtcgg
     1681 catcgacgtc tccgtttccg aggcgatcgc cgcggatatg ggcaaggagc tccaggtcgt
     1741 gaacatggac tttgacagtc tcatggccgc cctgcagaag ggcgacgccg acatcgtcct
     1801 cgccgctatc gaggtcaccg aggagcgtct cgaggccgcc gatttctccg atccctacta
     1861 caccgatctg cctccgatga tcctcgtcaa ggccgacaag gccggcgagt acaccagcat
     1921 tgagtccttc tccggcaaga gcgtcggcgc gcagaccggc acgaccaagg ccgatctcgt
     1981 caccagcgat atgcccggcg cgaatctcgt gggtctcacg agcgtgaacg accttgtcaa
     2041 ccagctcgtg tacgacaagg tggacgccat tgttgttgac ggcgccgttg cgcagcagta
     2101 tgccaagtcc aacagcgatc tcgtcgtggc ggacgtgagc ctcggcgaag ccttccccta
     2161 ctgcgtcgcc gtgcagaagg gcgacccgaa gggcctgctg ccctccatca acgccacgat
     2221 cgccaagctc attgccgacg gcaagatcga gcagtacatc gctgacgccg aagctctcag
     2281 cgatcaggcg atcgggtaaa ccgtacatcc ttctctttga agcgtgtgcg ccctgtctgt
     2341 gaaagcaggc agggcgcata tgtgccgttg atctctcccg cttccaaata cagaaaaggt
     2401 caaggaatag tattatgtgg tggtccaacc tgtttgccgg catgtctccg gcgaacttct
     2461 tcaatttctc cttcctgcca aagtatcttt ccttcttcgt gcagggactc gagtatacgc
     2521 nnnnnnnnnn catacatcgc cgtgttccgc tcgacgccga tgatggtgca gctctacatt
     2581 attttctacg gccttttcag cgtgatcaag atcccctcgg tcgtgattct gggcttcatc
     2641 gacctctccc gcttcattcc cggcgtggtg gcgcttgcgc tcaactcctc ggcgtatgtc
     2701 gcggagatca tccgcgcggg cattctcgcc gtggacggcg gacagaacga ggcggcgcgc
     2761 tcgctcggtc tcagctcgtg gcagagcatg aagctcgtcg tgctgccgca ggcgatcaag
     2821 aacattctcc ccgcgctcgc caacgagctc gtcacgatgg tgaaggaatc gtccatctgc
     2881 tcggtgctcg gcatgacgga gctcatctgg ggcgccaaga ccgttgcgac gacgacgtac
     2941 aagacgctct cgccgtacgt tctcgcagcg ctcgtgtact tcgtcatcaa ctacccggcc
     3001 ggcaaggcca tcgaagcgat cgaaaggaga atgcgccgtg gcgacagggt ctgaactcat
     3061 ccgcgttgag gacgtaacaa aaaaattcaa gggcggcgtc acagcgctca acgagtgctc
     3121 gctcaccata gcgcgcggcg aggtcgtggc gatcatcggc ccgtccggca gcggcaaatc
     3181 cacgctgctc cggtgcctga acctgctgga aacgccgacc tccggccata tctattttgg
     3241 cggcgtggat atcaccgaca aaaaggtgga catcaacctc caccggcgga aaatgggcat
     3301 ggtgttccag cactttaatc tcttccccca caagaccgtg atgcagaaca tcacgctcgc
     3361 ccccgtcacg ctcaagctca aaaccgagga cgaggcgaaa aagcacggca tggagctttt
     3421 ggaacgcatc ggtcttgcgg ataaggcaaa cgagtacccg aacatgctct ccggcgggca
     3481 gaagcagcgc attgcgatcg tccgcgcgct gtgcatggag ccggac
//
LOCUS       DAWAAE010000179         3508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10176_length_3508_cov_3.394150, whole genome shotgun sequence.
ACCESSION   DAWAAE010000179 DAWAAE010000000
VERSION     DAWAAE010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10176_length_3508_cov_3.394150"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          600 a         1128 c         1091 g          689 t
ORIGIN      
        1 cccctacagg aatatctgca ttgcgaccgt gggcggggct ctgctccgcc cgcgggaaga
       61 aattggttta ccgcaaaccc aatcgcctcc ctttgcacaa gggagcattt ttaaacatgt
      121 acaaccaacc gtatggttgg aaaaactaag cggaacgcac ccgtttggta gcatcaactc
      181 gggtgattgt atgtattttc cgcgtctgta cgagcttcgg gaagaccatg atctgacaca
      241 aaagaagatc gccgaatatc tgaccatgca tccggaggtc taccgccggt acgaagccgg
      301 tatccgggag atcccggtct gggcggtgat ccggctcgcg gagctgtatc acgtttccac
      361 agactatctg ctcggcctga cggacgaaaa gaacggccat gcgtaaagcg gcgctgctct
      421 gcgggacgct gtcccttgcc gttttcgtat ctttttatct cctgccggag agcgttctac
      481 tcccggcggc gggcgtctgc gcgcttcttt ccgcgctttc gctgctgctt ccgcgcggaa
      541 agggccgggc ccgcgcgcat ctcgcgctgc tcggcgcggc gatcgggctt tttgcctttt
      601 tcgtgcagga gcattttctc gccgcgccct cggacgcgct cgccggggaa aagcgcgtga
      661 tctccgtgcg cgtgacggac tacccggaca tttacgatac gagcgaatac ataacggtgc
      721 gtgtccgaga cccggacctg ccgggcgtga agtgccgcat cgcctcctac gatacggaaa
      781 atttcaccgc gctcacgccg ggcgacgagc tttccgtccc ggtaaagctg ctcccggcgc
      841 gcgagcgcaa cggccagagc gtggatacct acgccgcgca gggcatcttc cttcgcgcta
      901 cggcgaccgg cgagccggag atcaccggac gctgggactt ttcgtttctc tatgcgccga
      961 aggcgctctg ccgcgccatc ggggagatct gccgaagcgt gttcccgccg gatgtgcagc
     1021 cgtttctcac ggcgctcctc accggcgaca agaccgacct ttacgacgac ggcgcgcatt
     1081 actacgccct tgccgacgcg gggctcgcgc acgtcgtggc ggtgtccggc atgcacatcg
     1141 cctttctcat gggctttctc tttctgctga tcgggcggcg gccgtgggcg gtcgcggtga
     1201 gtctgccggt gctgctgctc tttgcggcaa tgaccggctt tacgccgtcg gtgacacgcg
     1261 cggcggtcat gcagttcttc cttctcgccg ccccgctctt cggccgggag agcgacggcg
     1321 taacgtcgct tttgacggcg cttgccctgc tgctccttct caacccctcc gcctgtgcga
     1381 gcgtcagtct gcaattgagc ttctcctccg tggcgggcat ctggtttctt tccgatcgga
     1441 tgtaccgcgc gctcagccgg cggatcgaag acttgaagat cgccgaaaac cggcacgccc
     1501 ggcgcgttct tcttttcgcc gcgggctcgg tttcgagcag catcggcgcg atggcactct
     1561 ccgcgccgct cacggcagtg cacttcggct cggtgtcggt cgtgtcgccg atatcgaaca
     1621 tcctctgcct gtggatcgtt tcggcggtct acatcggcgg gtacatcatc gtgctgctcg
     1681 gcgcgtttct gcccggcgcc gcggcgctgg ccggaagcgt tctcgcgtgg ggcgtgcggt
     1741 atatttacgc ggtgtccggc ttcctctcac gcctcccgtg cgcgaacgtt tatgtgcgca
     1801 atccgctctt tctcggctgg ctcgcgctcg tatacgcgct ctttttcgcc gcgtggctgc
     1861 gctctcggaa aaccgggcgc ttccgcgctc tcgcgccgat atgcgtttcg ctcgcgctgc
     1921 tctttgccgc cgcgggcatt acgaaactcc aatggagcga tacgctccgt ctcacggcgc
     1981 tcgacgtcgg gcagggggaa tgcatcgttc tcacgagcgg ggagcatgcc gcggtgatcg
     2041 actgcggcgg aagctgggtc acgcacgacg ccgggaaaag cgccgtgcag ttcctcggcg
     2101 gcgaatgccg gaagagcatc gacgcgctca tccttacgca cctccacacc gaccacgcca
     2161 acggcgcggc gcagctctta tcccgcgtgc gcgtcggcac gctctatctg ccgcgcgaaa
     2221 cggaggaaac atctctgctc gcggagatac tgaatgccgc ggagcggcag gggacgcggg
     2281 tggaatatgt gacggaggat atggcgctgc cgctcggaac ggctgggctt gccgtctggt
     2341 caccgctgct gccggaatcc gacgacgcga acgaaaacgg cctcatcatt gcggcctcgc
     2401 aggatgattt cgaggcgttc atcctcggcg acgcgccgtc caaggcggaa aaggcgctca
     2461 ttgcgagcgc gccgatgccg gacgccgagg tgctgttcgt tgcgcaccac ggctcgaaca
     2521 catccacctg caaagagctt ctggaagcga tcacgccgga aacggcggtc atttccgttg
     2581 ggtataatac gtacggccat ccgacgcaga aggttctcga ccggctggca aaatatgaga
     2641 tcaccgtgct gcgcacggat accgacggcg agatcaccat cgccgccggg gaagggtaaa
     2701 acgctatggc aaaaccgtcc ttcggcaaaa aggaaacgcc gctcgactac ggcgcgcttg
     2761 taaaaaatct ccgcgacaaa ggcccggaaa agctctatct tctctggggc gaggaggact
     2821 atcttctcgc ggacttcgtt tcgcgcctgc gcgccgcgtg cgtcggcgcg gagggagacg
     2881 agttcaacgc aaagcgcatc gactctcccg cgcctgcggc gaacgacatc gccgaagcgc
     2941 tcgacgccat gccgtttttc ggagacagga cgttcgtgga gctgcgcgga tttgacgtga
     3001 acaagtgccg tgacgagcgc accaccgcgc tctttgcgga cattccggac tggtgtaccg
     3061 ttgtcatcac gctcccgacg ggtatggagc cggacggacg gctctcattt atcaagcagc
     3121 tcaaatccgc gggcaaggcc gtgaacttta cggcgcagac cggcacgccg ctcaacaact
     3181 ggatcgcgcg ccgctttgag gccaacggca agcgcatcga ccgcgacgcc gtggaccggc
     3241 tcgtgttcct ctccggcgat cttatgaacc ggctcatccc ggagatcgca aagatctgcg
     3301 gctatgtccc cggagaccgc gtcacggcgc aggacgttga aaagctcgca catcacatcc
     3361 cggaggcgaa cgcctttcag atgaccgagg agatcgcccg ccggaactac gacggcgcgg
     3421 cggccaagct cgcggagctt ttcgccgagg acgccgagcc cgtcgagatc atgggcgtga
     3481 tcggctggca ggtgcggcag ctctatgc
//
LOCUS       DAWAAE010000180         3496 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10211_length_3496_cov_2.628596, whole genome shotgun sequence.
ACCESSION   DAWAAE010000180 DAWAAE010000000
VERSION     DAWAAE010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10211_length_3496_cov_2.628596"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          854 a          949 c          991 g          702 t
ORIGIN      
        1 acgccagcgc gagcagcaca gcgacagaga ccatggattt tgcgccgtcg atgatgagac
       61 ccggcacttc gctgaacttg aaatagccgc ggattacggg gtagccaatg gcgacaatgg
      121 tgccgacgag aatcggggtc atgaggctga acatgccggt ctcgatgcga atgacgatga
      181 tcgcgatgaa gaacacgacg aacggaagaa caaagctgag aaaatcgggc ttgacggcac
      241 tttccgtatc atcaagctcc ttcgtttcca tcgggctgga gcccggacgg atgagctcgc
      301 cgttttcttt tgcggccttt tcacgcttct tcatgaagcc aaaatcggga agaagctccg
      361 ccgccgtgag aagaccgacg aggacggaga gtatgcagaa gaagttgtac ggaatcgatt
      421 ccacaaacac ctgctgaccg tccagttcgg gaagaacaga ggaaatcaga ccgccaaata
      481 ccagaatgta gatcgtgatg gggacgaggg cgacgaccgg ctcagcggtg gaggagagta
      541 tgtagccgag cttctcgcga gagaccttct gcttgtccgt aatcggccgc ataatggcgc
      601 caacgccgat cgtaccgaag ccctgattga taacgatgcc ggacagaagc caggtgatga
      661 gcttcgcctt gcgcggtgtg tcaaagtgct tgtctccgaa ggaggagaac gcggcaaatc
      721 caccgccgcg cttgacggcc accatgaatg caccgaacac gatcatgtaa atgaagatgt
      781 caacattgct ggaaatgccg ttatagatgt acttatcgaa tacgagcatc aggaagtgga
      841 ggccgccggt atatatggag caggatacga tgccgaggaa aagcgcgagc atgatattct
      901 tagaccatag ggcaatgacc agcgtcagca ccggcgggat cagcgaaaga atactgggtt
      961 ccatagttcg atatttcctt tccctttaat tatttgttct ctttcacatt tctgtggggc
     1021 tgtcagagct ccgcgatggc ttcgatttcg cagagaacac ccttcggcag cgccttggcg
     1081 gcaacgcagg agcgggccgg gcagctgaca aagtacttgg catagatctc gttgaacttg
     1141 gcaaaatcag ccatatccgc gaggaagcag gtggtcttga cgactttggt catgtcgctt
     1201 ccggcagcgg cgagaatggc ggcaacgttc ttgcagctct gctcggcctg agtgtcgatg
     1261 gtgtcaccgg caggagcgcc ggtcgcggga tcgacgggga tctggccgga ggcaaataca
     1321 aggccgttcg tgacgatcgc ctgagaatac gggccgatcg cggcgggagc ggtttcggtg
     1381 gaaattactt tcatgacaac attcctcctt aagaataatg ttttcggaaa ttggcagcgg
     1441 aactgccgtg cttacgatgc cattataagc gcgttaaaaa tgtttagtca atataaaaac
     1501 gttcattcaa cgcgctttcg gatgagtgaa caatttttca ttgacgttat tgttgactat
     1561 cgatattgac acggcacggg gcatgaatta aaataataag ttgtgtataa aatagggaat
     1621 ctggtgtgac tatggaacag aaaaacagta aaaaaaccat gtccttcacg cgtatggacc
     1681 gcatggtgct ggaatcttat aagacgctgt gcgagggcct tgccaactat ctgggggacg
     1741 ggtacgagat cgttttgcac agtctggaaa actacgataa ttccgttatc aagatcatca
     1801 acggctacta taccggccgc aaggaaggcg cgccgatcac cgatcttgcg ctgcatatgc
     1861 tggaggatat ccgccacaac aacggcaagg actatatcgc ctattattca aaaaacaaaa
     1921 agggcgagcc catgcattcc acgacgatcg ccatccgcag cgagaacgcg aagatcgtcg
     1981 ggctgctgtg catcaacttt tatctgaata cgccgcttgc ctccgttata aacacgttga
     2041 tgaagacgga tgatggcgca agcgtcggcc gggaagagaa ctttgccgac agcccgtcac
     2101 agctcgtcgg cgatacgatc gcggagacaa agcgcgtcgt aatggcagac gccgatatcg
     2161 ccgtgcacga caagaacaag gagatcatcg cgcggctcta cagcaagggg tttttcaaac
     2221 tgaaaaacag cgttgtagaa tgtgccgagg cgctgggcat cagcaagaat accgtctatc
     2281 tccacatccg cagctgcaag aacacttaaa taaaagagac tgctgccagc taaagatgac
     2341 agcctctgtg ctgaaaccgc tcttgaagtg cgttctcctt tttacaatca ttttacgaaa
     2401 ctgcggtaac ggattttacc gtgcggcggt atgcttacgt cgtaccgaag ggttaatgat
     2461 ttgaaaaagg agaatctatt atgaaaacaa agaaaatttt cacgctggct atggccgccg
     2521 tactgacgct cgcgctgctc gcgggctgcg gcgctgatgc cccggctgcc gatcccactg
     2581 ccgcccctgc tacggacgcc cccgctacgg acgctccggc gaccgaggct tccgcttccg
     2641 aagcgcccgc cgctctttcc ggcagcgttt ccacgaacgg ctccacctcg atggagaagg
     2701 tcgtcaacat cctcgccgag cagttcatga acgacaaccc cggcgtcagc gttacttatg
     2761 accccaccgg ctccggcacg ggcattgagt ccgcccgcac cggcagctgc gatatcggcc
     2821 ttgcctcccg cgcgctcaag acgagcgaga ccggtctcac cggcacggtc gtcgcgctcg
     2881 acggcattgc gatcatcgtc aacaaggact gccccgtgga cgatctgacc attgaacaga
     2941 tcgccgacat cttcaagggc gagatcaaga actggaacga gctcggcggc gaagaccttg
     3001 agatctcctg cgtcggccgt gagtccggca gcggcacgcg cgacggcttt gagtccgtca
     3061 ccggcacgag cgatgcgtgc gtcctcgcgc aggagctgac ctccaccggc gctgtgatct
     3121 ccgctgtggc ggcgaacccc aacgccatcg gctatgcctc tctctccgcg gtcgaaggac
     3181 aggacggcat caaggcgctg aaggtgaacg gcgttgactg ctccgaggaa accgttctgg
     3241 acggcaccta cgccgtgcag cgtcccttcg ttctggtgac ccgcgagggc gagacgctct
     3301 ccccggcggc acaggcgttc tttgattacg ccaccggcac cgcggcgaac gacctcatcc
     3361 gtcaggccgg cgccgtcccc gtggcaaagt aaaaaaatcg catagccaaa gctccccgcc
     3421 aatgcgggga gactagcctg acgaaaaagc ctcgcagagt ttgcgcccgc aggcgcaaat
     3481 agagtgttaa tcattt
//
LOCUS       DAWAAE010000181         3413 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10470_length_3413_cov_2.882370, whole genome shotgun sequence.
ACCESSION   DAWAAE010000181 DAWAAE010000000
VERSION     DAWAAE010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10470_length_3413_cov_2.882370"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          717 a         1048 c          987 g          661 t
ORIGIN      
        1 gtcttgcaca gaccgctcaa ctacggaaaa agaagcggtc cgttgatttt tctgttgaca
       61 atacagaaat gctcatatat aatagcaagg cttgtgagat gctgacagag gattggaatc
      121 aacatgaatc tccacgaact tctcctgaat cccggctcac ggctgccctt ccggtgtgag
      181 ctttccactg accggctgga ttttcccgcc gttctctcct acaccgccgc ccccgtgggc
      241 gagggcttca tcgtgaacaa cgccggcgct ctgacgctgc ggggcacgct ctccgctgcg
      301 atgcgctgcg tgtgcgaccg gtgcgccgcc gagtttgacc gggaagtgag ctatccgctc
      361 gatgtcccgc tcgcctccga actgcaggac gaggaaaacc ccgattattt cctgctggag
      421 ggtgacgagc tcgatctgga agagctgctg gagaccgtgt tcatcctgaa catggacacc
      481 cggtttcttt gccgggccga ctgcaaggga ctctgctccc gctgcggcaa gaatctcaac
      541 gaaggcccct gcgattgcag gacagaatcg gatccaagac tggctgtttt ggaacagctg
      601 ttggacgata aaccatgatt atgcgctgtt caaagcagcc aaagtacgag gaggtgtcac
      661 catggcagtc cccaagagta aagtaagccg taccagacgc gacaagagac gcggcagcaa
      721 ctggaagctc accgctcccg ctctggtcaa gtgcccccag tgcggcgctt tcaagctccc
      781 ccaccgggcc tgcaaggcct gcggctctta cgatggccgt caggttctca aggtagacga
      841 ggagaaaaag taaccgagct taagaagagg aggctttgcc ccctcttctt ttgctatgag
      901 gagtttgtat gttcaacacg atctcgtccg tagaccccgg ctcccggctc gagggcaggg
      961 tgctccccgg cgacgaattg cgttatatca accggcacga gatccacgat gtgctcgatt
     1021 ataaatactg ggcgtatgac cgctcgctca cgctggaatt ccgggacgcc ggcacgatcc
     1081 gcctccgcaa aaaagagggc gaggatctcg gtctgaactt tgaaacgtac ctgatggaca
     1141 agccgacggg ctgctccaac cggtgcatat tctgcttcat cgaccagctg ccgcgcggac
     1201 tgcgcaaaac gctctatttt aaagatgacg acgcgcgtct ttcgttcctc acggggaatt
     1261 atatcacgtg tacgaatctc tccgagcgtg aattgcagcg catctgcgat ctgaaggtga
     1321 gcccgctgaa catttccgtg catgcgaccg atccggagct gcgcgcaaag ctcatcggca
     1381 acccgcgcgg caaagagatc atggacattc tccgccgctt cgccgcggcg ggcatattca
     1441 tggaatgcca gatcgtcgcc gtgcccggct ggaacgacgg cgcggcgctg cagcgctcgc
     1501 tggaggatct tgcggggctt tacccgcatg tgagcagcgt ttccatcgtg ccggtaggtc
     1561 tcacctgcca ccgcgaaaag ctcacccccc tctcccccta tacgcctgag caggcgcgcg
     1621 cgacgatcgc gcaggtggag tcgttcgcgg cggagtgctt tgaaaaatac ggcagtcaca
     1681 ttttctactg cagcgacgaa atgtatctgc gcgccggtct tcctctgccg gaggacgaat
     1741 attacgaaga ctatgcccag cttgaaaacg gcgtaggcat gctgcgtctg ctcgaaacgg
     1801 aatttaacct tgccctctcg ctggaggacg aggccgaccg gaaggaaacg ccgcgcttct
     1861 ccattgcgac cggcaaggcc gccgaaccgt atctgcgcgc gcttctggaa aaggccggag
     1921 caaacggcac ggtctacggc attccgaacg acttcttcgg ccacacgatc gaggtcgccg
     1981 ggctgctcac cgggcaggat atcatccgcc atctccggga taaggacatc ggcgagcggg
     2041 tatacattcc caaaaccatg ctgcgccacg gcgagggcgt atttcttgac gatcttacgc
     2101 ccgaggacgt atcgcgcgag ctcggcgtgc cggtcatccc gctcgaggtg gacggcgcgg
     2161 agctgctcgc cgcgatgcgt tttgaagaac tttgacaccc ccgaaaggag gattttccta
     2221 ttatggctaa acgacccctt gttgcgatcg tcggccgccc caacgtgggc aaatccctgc
     2281 ttttcaaccg cctgtgcggg aagcgtctct ccatcgtgga ggatactccc ggcgtgacgc
     2341 gcgaccgact gtacgccgag tgcgaatggg cggggcgcac ctttgatatc gtcgataccg
     2401 gcggcatcga gccgacggcg gacagcgaga tcctgctctt catgcgcgag caggcggaga
     2461 tcgccatcaa ctccgcggac gtgatcgtgt tcgtgaccga gatcggcacc ggcgtcaccg
     2521 ccgccgatca ggaagtcgcc gcgatgctca agcgctcgca taagccggtc gtgctgtgcg
     2581 tgaacaagat ggactccgtc ggtcaggtcg acccgggcat ttatgaattt tactcgctcg
     2641 gtctcggcga cccgatcgcg ctctcggcca tccacggcca cggcacggac gaaatgctcg
     2701 acgcgtgcat cgaaaacttc ccgcccgagg aagagggcga agaagaggac gatctcatcc
     2761 gcgttgccgt catcggcaag ccgaacgtcg gcaagtcctc gctcgtcaat ctcgtgctcg
     2821 gcgaaaaccg cgtcattgtc gcggatatgg ccggcacgac gcgcgacgcc gtggacacac
     2881 gcctggagaa caaatacggc aagtatatct tcatcgacac cgccggcatc cgccgcaagt
     2941 ccaaggtgga cgatcgcatc gagaagtttt ccgtcatgcg cgcgcagctt gccattgagc
     3001 gcgcggacgt gtgtctcatc atgatcgacg cgcgcgaggg cgttacggag caggacacca
     3061 agatcgccgg tcttgcccac gaagcaggca aggcgagcat catcgtcgtg aacaaatggg
     3121 atctcgtcga aaaggacacc cacacgatgg acaagatgcg caaggacatt tatcgcaatc
     3181 tctcgtttat gacgtatgcc cccgtcctct tcatctccgc gctcactggc cagcgcaccg
     3241 agcgcatttt tgagcttgtg aactttgtaa acgaccagag ctccatgcgc atcacgaccg
     3301 gccttctcaa cgacgtgctc gccgacgcgc aggcgcgcgt gcagccgccg tccgacaagg
     3361 gccgccgtct gaagatctat tatatgaccc agaccggtac gagaccgccg cac
//
LOCUS       DAWAAE010000182         3400 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10501_length_3400_cov_2.940508, whole genome shotgun sequence.
ACCESSION   DAWAAE010000182 DAWAAE010000000
VERSION     DAWAAE010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10501_length_3400_cov_2.940508"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    818..827
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          708 a         1023 c          958 g          701 t
ORIGIN      
        1 cacggtgacg ttcttcggcg cggtgttcac ccactgacgc ggcggaaaac ggcgtttatt
       61 ttagaagctc ttaccgaagc cttgctgaat catttaagga aatggcagat tccgatttaa
      121 aggaaatatg gaggtataga atacaaggtc tttgttttat tggaactggt gctgtggcgt
      181 gggttatggg tgataagctt gcaaaacgaa aggggaatta acctatggcg ctgataaaat
      241 gcccggaatg tgggaaagaa gtatcagata aagcagattc gtgtccagaa tgcgggtatc
      301 ctattagccg taaaagtgct cccccagaat caagcaggaa aaacaaaagc aaaatcatca
      361 ccgctatttt tgttatcgtt ttattgtttt ttgctgtgag catatttctg ccgaacacaa
      421 aaaagaaaag caattacgaa aactattcat attccagcga gagcagagcc tcgtatagct
      481 catctcatac atcaaaaacc gatgattcta cgatttttaa ccatttagaa ataacaaatt
      541 tttcttgtta cagcggtaag tattatggga aaatgcagtg tagtgtgaaa aacaataatt
      601 cgcatacagt aggaggatgg ttccgagtaa acttctatga tggcaacggc gccctgatat
      661 ataatcagct aatgtctttg ccggatgttg ctccgggaga atgcgtggta tgctcaacta
      721 caataccaag agacgattat ccatatgatt acgctaatgt agatttttct caggcaacgc
      781 tatccataga gaattagaaa tagcaaaagc cggaagtnnn nnnnnnngtc cgcggacttc
      841 cggcttttac acatatacac ggaggaatta tttcttccgt ctggtttctt cgataaactt
      901 cttccagccg cccttttcct tattaatgac tttcagcagc gccttgatct gatcgtcgga
      961 aaagacgttc gccatgcgcg cggcgttgcg ccacggcata tcgagcacgt acatgagatt
     1021 cccggcgttt tccttgctgc ccacaagatt catcacgcgg cgcgcggctt ccagcgcgtt
     1081 gtacagcccc ttgccaagac cgccggggag atatttcccg cgggagatgg cgtcgttgaa
     1141 gtccaccgcg ccgcgctctt cccagcgcgc gggcgggata tcgtgcccga gcagcgcggc
     1201 aaagctctcg tcggacacat gctgcacatc cgcgcggcgg taaacgtcaa attccggcgc
     1261 ggcgtattca tcgaagggcg cttcaccggc aacatgcacc gtgtgtacaa gccgcagatc
     1321 gcggcaggac gcgccgacgc agagctcgta ctcgccctct tccacgaccc agcggttttt
     1381 cgcgcagctc caaacggcga agctccgctc gccgagcggg agaaagaccg tcttttcctc
     1441 gccgggcagc agttccacgc gcgcgaagcc cgcgagcgag agcgcagggc gcagcatgcc
     1501 gagtgtcttg ctgcggacgt agagctgcgc gacctccgct ccggcgcggc tgccggtatt
     1561 cttcaccgtg aagctcacgc cgccgggcga aaattcgccg ggcgcgtagg aaaactccgt
     1621 atagctcaat ccgtagccga acggatattt gacgcgctta tgcgcgcttt cataataccg
     1681 gtagccgacg aacagcccct cgcggtactc gctcgtcgcc tcgcggcccg gataccacgg
     1741 cgcgcagggc gtgtcctcga gccggatggg catcgtctcg gccagcttgc cgctggggtt
     1801 cgcctcgccc gtgaggagcg cggcgcacgc gctgccgacg gcctgcccgc cgagatagag
     1861 atgcagcacc gcgcgggcga acacatccca cgtcatctcc accggactgc cgcactgcag
     1921 cacgacgccg acgttcggat tgacctccgc aatctcgcgc agaaggtcga gctggttttc
     1981 gcgcagtcgc atatgctcgc ggtcgtatcc ttccgtttcg ctgcactcat ccagaccgag
     2041 gaagagcagc accttatcgc ttcgcgcggc gagcgccctt gcccggcgca gcagccgcgg
     2101 cgcagcggct ccgctgcggg aaaagccctt ttcgtatccg gtgacgttca cgccggcagc
     2161 gcgcagcgcg tcgagcggcg cgtccacctg cgtggggttg acacgcgaac tgcccgcgcc
     2221 ctggcagcgc ggcacggcgg caaaatcgcc gatgacggcg acgcgctcgc cctgccgcag
     2281 cgggagaaat tccttttcgt ttttcagcag cacggcagac tccgccgcgg ctttcgcggc
     2341 gagcgcatgg tgcgccgtgc cgtcgaaaac gccgccgcac gcgagcgctt tctgcgcggt
     2401 aaaaataaag tccagcacgt gatccacctg ctcgtccaga agggcctcgt cgagcgtccc
     2461 gttctgtacg gcctcgacga tgtgccggtc ggtctcgccg ttggaggccg gcatttcgag
     2521 cgacgatccg gcgcgcacgg cggcgacgcg gtcgttgttg ccgccccagt cggagacgac
     2581 gagcccgtca aagccccact cgccgcggag aatatcgcgc agcaggtgct cgttttcgtt
     2641 gcagtaagta ccgttcagcc ggttatatgc tgtcataaga gagcgcacgc cgccctcttt
     2701 tatggcggtc tcaaacgccg gaagatagat ctcgcgcagc gtccgctcgt ccatgacgga
     2761 atcggtcgcc atgcggcgca gctcctgcga gtttgcggca aagtgcttca cacacgcgct
     2821 cacgccggtg ctctgtatgc cgcggatcat cgccgcggcg agcttgccgg agagcagcgg
     2881 gtcctcggag aaatactcaa agttccgccc gcagagcggg ctgcgcttga tgttcacgcc
     2941 cggcccgagc accatgccga cgccctgcgc cacggcctcc tcgccgagcg cgcgtccaac
     3001 ggcagagagc agcgtttcgt cccagctgtt cgcaagcgcg ctcgccgtcg gaaagcacgt
     3061 cgccggaacg gagcggccaa gacccagcgc gtcggaatcg ctctcctgct tgcgcagacc
     3121 gtgcggcccg tccgtcatca tctcggacgg aatgccgagc cgttccacgg ccttcgtgtg
     3181 ccagaaatcc cgtccggacg tgagccccgc cttttcctcc agcgtcagct tggcaatgag
     3241 atcggcgtat ttcatatgcg cgccctcccc acactataat tataattaat tgcgtcgatt
     3301 gacaagggca cacgcggttc tgccgggcgg aaatgccctc atactgcgtc aaagcccttg
     3361 agaatacaca aagtattctc tgcggccttt tccttgtctg
//
LOCUS       DAWAAE010000183         3305 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_10797_length_3305_cov_2.829538, whole genome shotgun sequence.
ACCESSION   DAWAAE010000183 DAWAAE010000000
VERSION     DAWAAE010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10797_length_3305_cov_2.829538"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          947 c          908 g          705 t
ORIGIN      
        1 aaagaagccc tctcaaacca tgccgtatgg catagtttga gagggcttcc agtttgtttt
       61 tttccgcgat aaacgataaa ttgacttggt gcctttcatc aaagaatatt tttccgtgtt
      121 cttcgcctgg atcgccgcaa ttcgttttgg attgcggcgg cttttcacat tttcacttgt
      181 tttttctgtt ggatatgcta taaatataaa taattaaatg taaatcagaa acggagcatg
      241 gccatgaacg attttatcat agaaaatgcc cgtccggagg acgccgcggc aatactggac
      301 tacctgaaaa tcgtcggcgg ggagacggac aatctcaaca tgggtgcgga aggtatcccc
      361 attgccgtgg aagttgaaga agcgtttctt cgttccatgc tcgattcgtc cgacgacgtg
      421 atgtacgcgg caaaagcgga tggcgagatc atcggcatcg ccaacgtcag ccggctgaaa
      481 cggcgcatga gccaccgcgc cgcgatcggc atcagtgtcc ggcagtgcgc gtggcatagc
      541 ggcgtcggca cggcgctgat ggaagcgctc cttgctttcg cccggaaaaa cggcatagaa
      601 cagctggagc tcgaggtgcg ctccgataac gaccgcgcca ttcgcctgta tgaaaggttt
      661 ggatttacaa agatcggaac gatccccgca tttctcaagg tcaacggaga agtttttgcc
      721 tccgattaca tggtgctccc cctgaaaaac aacgcctgag acagatgcat tacgttcagg
      781 ccaaagggat actctccgcc ggaaacggaa tgaatctcta ccgcggctgc acgcatggct
      841 gcatttactg cgattcaaga agccgctgct atcatatgga tcacgctttc gagaatattg
      901 aagtcaaaga aaatgctctc acgcttctgg aagacgcgct ccgacgcaag cgcagcgcct
      961 gcatgctcgg caccggcgca atgaccgacc cctacatcct gcttgaatcg gagctcggct
     1021 atgttcgcgg cgcgctctcg ctcgcgctcc ggtacggctt tggcttcaca ctgcaaacaa
     1081 aatccgaccg cgtgctgcgc gatattgatc tcctgcaggc catcaacgag aggacaaaat
     1141 gcgtggtgca gatgacgctc accacatacg acgaggcgct ttgccggaag ctggagccga
     1201 atgtctgcac cacctccgcc cgcttcgccg cgctgcaaaa gctccgcgag gccgggatcc
     1261 ccacggttgt gtggctctca cccattttac cgtttatcaa cgacacggag gaaaacattc
     1321 tcggcattct gcgctattgc gccgaagcaa aggtccaggg cgtcatctgc ttcggcatgg
     1381 gagtaacgct ccgcgagggg aaccgcgaat acttctttgc gcagctcgac cgggtgtttc
     1441 cgggaatgaa ggagcagtac atccgcgcct tcggtctttc ctatgaaaac accagcccgc
     1501 gcaatgccga gctcatgcgt ctctatcacg ccttctgtga ggcgcacggc atgatgcacg
     1561 ataatacgga aatattcacc tacctacgca tattcacaga caaatccgac gaaacgcagc
     1621 taagcctgtt ctgacgtgta cttaccgaat tacaaaaaaa cttatttccg gaaacggagg
     1681 cgcttatgac ataccttctg aaaaatggca agatctatga cgggacgggt gcggacgctt
     1741 ttctcggcga tatactcgtc cgcgacgacc gcattgaaaa gatcggcgag ggacttatct
     1801 gcgacggagc gcaggtgatc gatcttcagg gtctatccgt gtcctccggg tttatggacg
     1861 ctcactcaca caacgactgg ttcgctatcc gcaaggatcc ccggccgttt tttgagccgt
     1921 tcatccgaca gggcatcacc tcgttcatca ccggcaactg cggtctttcc gccacagggt
     1981 tcactccgga tacgccgtat cttgataaga tcggcggcgg gcttttcggc taccgcgggg
     2041 atgagaccgg cgtgtacccg tcggccggcg cactgctcga cgccgcagac ggacgctccc
     2101 cctgcaatat tgccgttctc gctggccatt gctctgcgcg cgccggtatc accggctttg
     2161 acggccgcga tctgacgcct gccgaagagg agcagatgct cggtactatg gagcagagcc
     2221 tgaaggaagg cgcctgcggc gtgtcgctcg ggctgatgta cgaaccggga ctgtatgttt
     2281 cgatcgaaga gctgaaaaag gtcgctcgtc tcgccgagcg gtatgacctt ccgatgaccg
     2341 tgcatccgag agcatgcagc gccgtttcca tgacctatcc cctgcttggc cggccgcacc
     2401 ttctccgcgc gctcgacgag ctggtggaga tcacgcgcgg cacgcacatg aagctccagt
     2461 actcccacgc gatcttcgtt ggacgccgct cgttccgctg taaggatgag ctcaaggaga
     2521 tcatttatcg gatgcgggaa aacggcgtgg acgcgcagtt tgatatatac agcgagctgc
     2581 tcggcgtatc tgtcatcacg gtcgtaatgc cgacgtggta tcaggcgctc agtgcggaag
     2641 aaaaacgccg tcctgtgaac atgctccgct tttccctgct cgccaaaatg agcatcgcgc
     2701 ttctcggctt tggctgggat gacatcacca ttgcttacgt cggcgagggg aacgagcgat
     2761 acgagggaaa gaccgtggcg caaattgcca gggaaatggg aaaaaccgac atcgacgcct
     2821 atctcgatct ctgcgaaata agcgatttca agggccgtgt caacatgggg ccgtacagca
     2881 cgccggagat cgtcagcgat ctctcccgcg acgatgtgtg cctttatatg actgacgcct
     2941 gggtcgagcc gcacggcgtg cagaaccccg ccatttacga ctgcttcccc aaatttctca
     3001 aatactccct caacggcacc ggcgacacta tgccgcggac gatccggcgg cgtagccgac
     3061 cggttctcca ttccggagcg gggatacgtc cgcgaaggct atttcgccga ccttaccgta
     3121 ttcaacgaag ataagctcaa agccggagaa gaggatcgcg gacattcctt cggcatagaa
     3181 aaggtcttta tcaacggcca gctcattctt gacggcgaag cgctcaacac cgacgccatg
     3241 cgcacgagcg gccgcgcgat gcgcgccgcg aagcgataac cggcatatta tgagaaaagg
     3301 atccg
//
LOCUS       DAWAAE010000184         3235 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11033_length_3235_cov_2.927673, whole genome shotgun sequence.
ACCESSION   DAWAAE010000184 DAWAAE010000000
VERSION     DAWAAE010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_3235_cov_2.927673"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          672 a          927 c         1040 g          596 t
ORIGIN      
        1 tcagcccggc gatctcggtg cggccgatgg tgagattgaa gtcgtccacg gcgcgaagac
       61 cgccgaaatc gatgccgaga tggcggcatt cgagcacggg tgttttgtcc agatcgcgct
      121 cgggaatgat ggctgtgctc gggaattcga ccatttttcc cttttcaaac tttttagaca
      181 tcttccctct cctccttccg gccggggatc acgcggcgca gcagcgcctt gaaatcgtcg
      241 ttgttcgtcg cgagcatcac gaggatgagc acgatggcgt agatcagcat gcggtaatcg
      301 gcaaagctgc gcagcgcctc gggcagcgcc gtgagcagcg ccgcggcgat gaccgagccg
      361 agcatattgc ccagcccgcc gaacacaacg aacacgagaa tgaggatcga cgcattgaaa
      421 tcgaacttgt tcgcggcgat cgtggagtag ttcagcgcga agagcgcgcc cgccgcgccc
      481 gccatggaag cggagatgac aaaggcggtc attttgtagc gcgtcacggg gatgccgacg
      541 ctctcggcgg cgatgcggtt atcgcggatg gcgagaatgg cgcgccccgt gcggctgttc
      601 acaagatgga agatcacgat gaggcagagc atcacgagga taaagccgcc ggtgaacgtg
      661 gcgatcttct gcacaccgcc gatacccatc ggtccgccga gaataagctt tccgccctcc
      721 tccagatttt cgagcgcctg caggaagcgg atgtggtagc cgcggctgtc caggccaata
      781 taaaggttcg tgaaaatgct cttgatgatc tcgccgaagg cgagcgtcac gatggcgaga
      841 tagtcgccgc ggagacgtag caccggaatg ccgatgagaa agccgatgat ggcagcgaag
      901 atcgcgccga tgaccattgc gatggcgaga cgcagcggag cgctcgcaat aacgccctgc
      961 agcgacaccg ccgccgtcgt gcccatgaag gcgccgacgc tcataaagcc cgcgtggccg
     1021 agcgacagct cgcccatcac gccgacggtg aggttgagcg agaccgccat aacgacataa
     1081 gcgcagatcg gcacgagcat gccgcgcgcc gtggaggaaa gcaccttctg tccggagagg
     1141 atctggagaa cgatatacgc agcgatcaca aaggcatatg tgatgcactg ccgccggttg
     1201 attttcattc gggctaaaac ttttttcatc gtacccacct cacactttct cgcggacctg
     1261 cttgcccagc agaccggacg gcttgacgag cagcacaatg atcagcaccg cgaacaccac
     1321 ggcgtccgac atctgcgtgg agatgtacgc cttggccatg atctcgatga ctccgagcag
     1381 cagtccgccg agcagagcgc cggggatcga gccgataccg ccgaacacgg cggcggtaaa
     1441 cgccttgatg ccgggcatgg agccggtcgt ggggacgagc gtcggatacg ccgagcagag
     1501 cagcacgccc gcgaccgcgg caaggccgga gccgatggcg aacgtcatgg agatcgtcgc
     1561 gtccacgttg atacccatga gccgcgccgc gtccttgtcc tcggagcagg cgcgcatcgc
     1621 cttgcccatt ttggtcttgc cgatgaagag cgagagcgcg accatgatga cgacgcacac
     1681 ggcgatcgtc acgagcgcga catacgaaac acgcagctca ccgccgaaga gggcaacgcc
     1741 cgtctcgccg ctcaccgact tgaagaagtt cgggaacacg accggactgg aaccccagag
     1801 cagcagcgcc gcgttctgga ggaagtagct cacgccgatg gcggtgatga gcaccgcgag
     1861 cgacgttgcc gcgcgcagcg gcttatacgc cagcttttcg atcgtcatgc cgagaacggt
     1921 gcagacgatc atggcaagga tcacgccgac gatcggcggg agattaaact tggtgcaggc
     1981 aaaaaagcag atatacgcgc ccaccataat gacgtcgccg tgggcgaagt tcagcatttt
     2041 cgcaatgccg tagaccatcg tgtagccgag ggcgatgatc gcgtagatgc tgccaaggct
     2101 gacgccgctg atcagaaagt tcaggaaact caaggctgac acccctttcg ggtcaatttc
     2161 tttctctttt tctctctaaa aactaaaata cggggacagg ggaaaatgcg catcggaaaa
     2221 aggcttcggt gcggcctctt tccgctgcgc gccttcccgt ggggaatggg acggaggggt
     2281 accctccgtc ccatagctgt ttcgtgggtc gggctttttc ccgatcagtc cagaccgacg
     2341 taagcgtcgt tctggatgac catgcccttc gggctcttgg tgacctggcc ctgctcgttc
     2401 caggtcatgc cgtcgccggt cagaccgtca aacgtcatgg aggtgaactg ctcgatcatc
     2461 ttggtgcaga ggtctgccgc gctcatgtcg ggcgtagcgc cggcggcttc gagagcctgc
     2521 gcgtaggcat aaacgcagtc ataggcgtcc gcggcgaact ggttgggaac ttcgccgtac
     2581 atttcctggt acttcttgac gaaagcggcg gtgcgctcgt cggtggagtc ggcgttgaac
     2641 ggggtcagga gcatgacgcc ctcggcgaga gaggtatcaa agcccttcat agtgaggatg
     2701 ccgtccatgc cgtccacgcc gaaccattcg ggtgcataac cggcggctgc ggcctgcgcg
     2761 aggatgaggg aagcgggctg gtcgtacacg ggaaggaaca cgagttccgc acctttcgac
     2821 tgcgcatcgg cgatctggac ggagaagtcg gtgtcgttgc cgtcggcgaa ggtggtgtcg
     2881 ctcacgatct cgatgccgag cgtatcgcag gcggccttga aggtctcata aatgcccttg
     2941 gagtaaacgt cgtcgttctt ccagatgaca gcgaccttgg tggcgaggct cttgtcggcg
     3001 atgtactgcg cggaggtgct gccctggttg gggtcggtga agcacatctg gaactggtta
     3061 tccttgccct cggtcgtggc gacagaggag gcggacggag tcagcgcgaa gatacgatca
     3121 gcaaacgtat cgccggaggt aacttcgcag ggcttggagg tgacggagcc gagagagatc
     3181 tgcatgcccc agtccttcag ggtgttgtag gcgttgacgg ccttttcggg gtcgt
//
LOCUS       DAWAAE010000185         3200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11173_length_3200_cov_2.857552, whole genome shotgun sequence.
ACCESSION   DAWAAE010000185 DAWAAE010000000
VERSION     DAWAAE010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11173_length_3200_cov_2.857552"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    518..527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          539 a          947 c         1061 g          643 t
ORIGIN      
        1 aagtattttt gacaagctct caaacgagac ccacttcgtt gggctctcgt ttgaaggggc
       61 tgcggctcgc cgcagcgcac tcgcttacgc tcgcacgttt gcgagccgaa aagtattttt
      121 gccgacgtac acgccgccgg caaaaatgct ttacacttta tttgcgcctg cgggcgcaaa
      181 ctctgcgagg cttttttgac aggctgtaag acccatcttc ggatgggtct tttccttttg
      241 tttgcgccga ttatttccgg tcatttccaa ggcggaaaaa ccccgatctg acagcgccga
      301 acgaccgcct ttactctccc ggcagactat gcttgtccta tattctatgg ggggtaaacg
      361 tatgtcattg ggagataaga ccgtgctgct gcgaaatccg tggaaagggg agacgctgcg
      421 catcaaccgt cccgcggcgg tcagggccgc ttcgctcggc acgcatttcg cgctgggttt
      481 tgtgacgggg tgcgtgcggg tgttcggctc gtgcgcgnnn nnnnnnngtt cggcgtggca
      541 atggcggcgc gctcgggcgg cggtctcggc gggctcgcgt gcgtcctcgg tgcggcggcc
      601 gggtatcttg tttccggctc gctcgcgtgg gggctgcggt atatggcggc gctcgtgctc
      661 gtgtttacaa cggcctttct cctgcgtgaa acagcgatcg cgcaccggtc gtggtttatg
      721 cccgccgtcg ccgccgccgt gatgggcgct accggcgctc tgaacgcctt tgataagctt
      781 ttgcagcttt ccgtggcggt cgatctcgct accgaggtcg tgctcacggg gctttcggcg
      841 tatctcttcc atatggcgct caccgccgag cggctgcggc tggagcggga ggagctgcgg
      901 cgggaagcgg gcggcgtgct gatcctctgc tgcgcgctga tggcgctcgc gcgcgtcacg
      961 atcatggacg ttctctgtct tggccgtgtg gcggcggtgc tgtgcgttat ggtctcctcg
     1021 ttcggcgcgg gcgcggcgga gggagcgctc atcggcgtca tcaccgggct gtcgatggat
     1081 ctcgcgtccc ccgcaacgcc gatcttcacg atctgctacg gcttcgcggg actgatgtcg
     1141 ggcgtcctca agcggcaggg acggctcgtg tttctgctgg tcttcctcgc gtccaacacg
     1201 ctcgccgtgt tctggaactg ggaggcgaac ccctgcctcc cggcactgta cgaggcattc
     1261 tgcgcttccg tgatcttctt tgtgcttccg gccaagacga tgacgttcat cgtctccatg
     1321 gtgcagcgtc ccgctctcgg cgagggcgag agcggcatgc gccggtacac cgccgctcgg
     1381 acgcgcgctc ttgcggacgc attctgcgaa ctgtacgaaa cggtgcgccg cagcgcagag
     1441 agcaaggaaa acgacgccga cattgccacc gtgtacgacc gcgccgcgga ggaggtctgc
     1501 gtccgctgcc cgcgaaagga aaagtgctgg caggaaaact atatggacac gctcacgctc
     1561 ctgaacgacg ccaccgctgc catgcgccgc cgggggcatc tggagctcgc cgatcttccg
     1621 gcgcgctttc gggagcagtg caaaagctct gcgccattca ccgcagcggt caattccgag
     1681 ctccgtgcgc tcatctaccg gcggcagctc cgctcgcggc tgcgggagaa ccgcatggcg
     1741 gcttacggac agtaccggta tctcgccggg gtgctcgacg cgatcagcga ggatctgcgc
     1801 tgcgcgaacg gctcggacac gatcgccgag cggcgtcttg tgcggtatct caactcgctt
     1861 gatatcgacg cggaggtatc cgtcttccgc gacccgacgg ggcggctccg cgccgtgatc
     1921 gagagtgcga gacttccgat cctctttcgg gaagcggact atatgcagga ggtatccgat
     1981 gcgctgggcg tccgcatgtg ccgcccggcg tcgtccaacc agaacgatac gcgccttgtt
     2041 ctgctggagg ccgaaccgct cgccgtgtcg gtcggcgtgg cggcggtgaa aaaggaggga
     2101 gagccggtct ccggcgaccg ggggacgtat ttcaaaaccg agcagggcgt gctgtgtgtg
     2161 ctgctctcgg atggtatggg cagcggcgag caggcggcga aggaatccat ctcggcggtg
     2221 cgcattttag agcgcttcct ccgctccggc gtagaacccg ccacggcgat gaagatactg
     2281 aactccgtta tgcttttaaa aagcagcgag agctggggct atgccgccat tgacctcatg
     2341 tgcatcgacc tcttcaccgg tcaggcgggg ttttataaat acggcgccgc gccttcgtac
     2401 atccgcagcg gccgcaatgt ccgccgcgtc aagggcatga cgatggcggc gggcgtgctc
     2461 gcgggagagg gcgagccgcc ggacgtcgtg cggctgcaca tccggcccgg cgcgctcgct
     2521 gttatcgcaa gcgacggcgt tgtaacgcgg gaggatgata cgtggctgcg gcgtattctg
     2581 ctcgacgacg acggcacgga tatgcgtgct ctggcccgca gcgtcatcca cgaggcgggc
     2641 gagcgctacg gctacaagga tgacatgacc gtccttgcca tccggctgga ggagcgcaaa
     2701 tgagcggaag agagcgtctg cggcgcggct ggctgcggtt tggaaaagtg ggccataaac
     2761 cgaataagga tggcaaaaac cggttatgct tggagaagaa ccgctgccgg gaggaggaag
     2821 agttccccgt ggtcaaagga atctccaaaa gagtcatcgt cgtaaaatcg ccggacccga
     2881 aaatattcga gcaggccata ttcatcatcc gcgaggattt tgccggtcag tccggcgtga
     2941 gcgaaaagga cgtgctgcgc gaagcgcgcg ccgcggcgaa cagctatctc ggcggcggaa
     3001 aacgaacgac cggcaagctc tttgcccggc tgcgtgcgcc gctctatgcc gcggcgggcg
     3061 ccatcgctgg ggtcatcgcg tggtttgccc tgcatctggc gcatctctga aaaatcggag
     3121 ccggtcgatt atgaccggct ccgatttttc cggttgcgcg atgcgcggcg ggatgataag
     3181 ataaacgcat aatacataaa
//
LOCUS       DAWAAE010000186         3192 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11199_length_3192_cov_2.684412, whole genome shotgun sequence.
ACCESSION   DAWAAE010000186 DAWAAE010000000
VERSION     DAWAAE010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11199_length_3192_cov_2.684412"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1212..1221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          669 a          925 c          903 g          685 t
ORIGIN      
        1 gggtcttgtc ctcgtacatt tgctgatcct ctctttgatt ctgcccttgg gcttgatatt
       61 gcgttcagct ttcgctgttg tcgcctgaag aaattatccg ggagagcttg cgccggatcc
      121 gctgtatggc gttgtctacc gctttttcgg ttcgtccgct ggctgcggcc atgtaagaat
      181 aagaatatcc cgctaaatac aacggcagga tcttctgttc aaagggtgac aagcaccggg
      241 aaaatgtctg gaaaaattcg tctgtgcgct ctctcgccaa aacctgttct tccggggagt
      301 tccgtaaatc aagggccgca tacataccgg gacggattcc ttcgctgccg aacgcatcct
      361 ccagcgatac gccgctgttg agagggatat gcttcaaaca agacgccgat ttgatcgcac
      421 tgaagatccg ccgccggatg cacagttccg catacgcctt gaaagggacg ccctgcgcgg
      481 gtctgtactc ccggatcgcg gagagaaggc caagcattcc ctcctgcgtg agatcttcgc
      541 tgtcccctcc ggcaagaaaa tacggtcttg cgcagatccg gacaagctga ttgtaccgag
      601 ctgctaacgc atcttccgcc ccgaccgagc cggaaacaga catttgctga agctgctcat
      661 cgctgaagca ctgaaaatct tccataaaaa aaccatttta taaacggatt atatttgttt
      721 ttatcaacaa agtcaagaga aatatgaatg aattatgaag ttttttccaa attccccggt
      781 aaaatcacag atcgagctgc tgctgaccgt gaaacgggat gccgagatgc tcatatgcct
      841 tctgcgtaac gcaccggccg cgcggggtac gggtgagaaa tccctgctgc agaagatagg
      901 gttcgtagac atcctcgagc gtaacggact cctcgttgat cgtggcggcg agcgtttcca
      961 cgccgaccgg accgccggaa taatactcga tgatgctgcg gagcatgcgc cggtcaagag
     1021 agtcgagccc gagatggtcg atctccagcc ggttgagcgc ctcgtctgcc acttgccttg
     1081 tgataacgcc gtctgccgtc acctgcgcaa aatcgcgcac gcggcgaagg atgcggttgg
     1141 cgatgcgcgg cgtgccgcgg ctgcggcggg cgatctccag aatgccctcc ggctcggcgt
     1201 ccacgttcaa gnnnnnnnnn nctgcggcgg gcgatctcct ccgccccgtc gtgctcaatg
     1261 cttatgccga ggataccggc gctgcgctca acgatgtggc ggagctcctc cggcgtatac
     1321 agctccagac ggagaatgac gccgaaacgg tcgcgcagcg gcgcggaaag ctggccggag
     1381 cgtgttgtcg cgccgatgag cgtaaatttc ggcagatcga gccgaatgga gttcgcggaa
     1441 ggtcccttgc cgatgatgat gtcgatggcg tagtcctcca tcgcgggata taggatctcc
     1501 tcgaccgcgc ggttcatgcg gtggatctcg tccacgaaga gaatgtcgtt ctcctgcaga
     1561 tttgtaagaa gcgccgcaag atcgccgggt ttttcaatgg tcggcccgga ggtgatacgc
     1621 atatttacgc ccatctcgtt ggcgataacg gcggcgagcg tcgttttgcc gagtccggga
     1681 gggccgtgga gaaggacgtg gtcaagcgct tccccgcgca tctttgccgc ctgaatgaaa
     1741 atgcggaggt tttcctttgc gcgctcctgc ccgatatatt cgttcagata ccgcgggcgg
     1801 agcgatccct caccggcatc ctccggcaga gccgtggatc gggttatcaa ttcattttcc
     1861 tgctccgcat ccgaaaagct gatcgccaag ctgctccgcc tccttttact tcatcagttt
     1921 cttcagcccc tgccgcacga tatcctcgac ggacagattc tgaatatcca gtcccttgag
     1981 cgctgcggcg atctcgtttc cgccgtagcc gagcacactg agcgccgccg ccgcatcgga
     2041 gagcttgtct ccgccggatg ccgccgcggg cgcataagcg ccggatgagc cgaaagagct
     2101 gtccccgaca gcgccgccgg agaatttatc tttcagctcc atgatcacgc gctgcgcgat
     2161 ctttttcccg ataccggggg ctgccgtcag cgccttctcg ttgccggaca taatagcgag
     2221 agagagggat tccggcgtgt tcgcggagag gatcgcctgc gcagccttcg cgccgacgcc
     2281 ggaaacgccg atcagcagct caaagcagcg cttttcctcc gctgtcgcga agccgtaaat
     2341 gtcaaagcag tcctcacgga tgatcgtgca ggtataaaac cgggcctttt cgccgatgcg
     2401 gacccgcgag agcgagttga ccgtggtatt gacagcatat cccacaccgc cgcagtccac
     2461 gacggcgacg gggccgtcga tcagggaaac gacgccttct atatagtaga acacagatcc
     2521 cggtctcctt cccttgttaa aagcgatgta gcgctgcggg cgtggcagag cgcgatagcg
     2581 accgcgtccg ccgcgtcatc cggccgcgga acggcggtca gatgtaaaat gcggcggacc
     2641 atgtccatga cctgccgttt ttccgccatg ccgtatccca cgacggcctg cttcacctgc
     2701 atgggcgtat actcatacac tggcacgccg cagcgaaacg cgcagagcaa tatgacgccg
     2761 cgagcatgcg caacagggat gcctgtcgtg atgtttttac tgaagaaaag ctcctcaatg
     2821 gagatagcat ccggatcgca ggagcggatc agctcctcga gatccgaata gatccggtca
     2881 aggcgcgatg agagcggggt atgcgccgga gtcgtgatga cgccgcagga aatcatcctc
     2941 tgatgcgtgc cggaggattc caccagccca aagccgacgg tagcgacgcc ggggtcaatg
     3001 ccgagaattt tcataccgct cccctttctt atcctattga ttctaccata aaaccgctcc
     3061 catggcaatg cttttccgag agaggaaaaa cgggttggag caattccaac ccgtttgagc
     3121 ttgtcaaaaa agttttttga caagctctca aacgagacac acttcgttgg gctctcgttt
     3181 gaaaaggctg cg
//
LOCUS       DAWAAE010000187         3121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11457_length_3121_cov_3.575995, whole genome shotgun sequence.
ACCESSION   DAWAAE010000187 DAWAAE010000000
VERSION     DAWAAE010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11457_length_3121_cov_3.575995"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          680 a          923 c          922 g          596 t
ORIGIN      
        1 acatgttcaa ctgcgacaac ggcacctacg acgcatggta ctgggcgcat ccgccggtct
       61 atatgccgcc caactgcaac attgcggatg tcgagcatat caagaacttc accaccaagc
      121 cggtcgtctg cgccggtaag atggagcccg agttcgctgc caaggagatc gccgagggcc
      181 gcatcgacgc gatgggcgtc ggccgtcaga acctcgtcga tcccgaatgg atcaacaagc
      241 tccttgagga ccgcgaggag gagatcaagc cctgcatcaa ctgccacaat gcctgcttca
      301 acttcaactg ctccaagggc acgcccaaca tgcagccgat ggacgatgcg ctgcatctgg
      361 cccgctgcgc gttgaccccg cccacgatgc agcacaacaa gtataagatc gtcccgacga
      421 agaaccccaa gaaggtcgcc atcgtcggcg gcggtatcgg cggcatggaa tgcgcccttg
      481 tcctcaagaa gcgcggccat accccggtca tctacgaaaa gagcgataag ctcggcggca
      541 tgtacaacta tgcctccgcc atgtccttca aggaagcgga caagaagctg ctcgagtggt
      601 acgaccgtga gctgaagaag gccggcatcg aggtccacct caacaccgag atcgacccga
      661 tcaacccgct cacccgccag tatgacgagg tcatcatctg caccggcgcc gtgccgaaga
      721 agctccccgt gcccggcttc aacaagacga tcacgtttga agagctgctc accggcgata
      781 aggaccacgg cgacaaggtc gtgttcttcg gcggcggcca gtccgcgtgc gaagcggctt
      841 acgagctcgt gctgcagggc aagcacccga ttattgtcga atacgccaat gacctcatcg
      901 ccacgcccgg cacctgcctt gccaacgcct ccttcctgcg cgaaatgctc gtgttcaaga
      961 aggttcccat ctatctcgag cactccatct gcgagatcgg cgacggctac gtcatggtca
     1021 agccgcgcag cggcggagat gccttcaagg tcgagtgcga cagcgtcgtc aacggtctgg
     1081 gcttccgtcc cgcgccggtc gcgcctgcgg gcaagcatgt ccgccaggtc ggcaccgccg
     1141 cgaagtgggg caacctgcgc aacgttatct ggggcgcatg ggatgtctgc atgaagatct
     1201 gatcttacat acaaacgcat aagcgaaaca aaccggcgga gggacgatcc ctccgccggt
     1261 ttcagactgt cgaaaaagcc tcgcagagtt tgcgcccgca ggcgcaaaga aagtttaaaa
     1321 catttttgcc gatgacatgt gcatcggcaa aaatacttct cggctcgcaa acgtgcgagc
     1381 gcaagcgagt gcgctgcggc gagccgcagc tttttcaaac gagagcccaa cgcagtgggt
     1441 ctcgtttgag agcgtgtcaa aaatactttt ttgacacgct caaacgatcc ctccgccggt
     1501 tttcgctgcg gtcagtttca attcaaagac gataataggt gaactcgcct ttcgtgagaa
     1561 ggctgtgttc ggaatcataa acgcacacgg tagccacgat gatgcggctt ccgcggcgga
     1621 tcacgtcgcc ctcggcggtc agcgtgcccg gcttccccgg atggagaaac acggtagagg
     1681 cgttttgcgt tacgcagcgg atggtctccc cggaatcgag tgtgcgggcg gcataccctc
     1741 cggccacatc catcatggtg aagagcaggc cgccatgtac gatcccgtac ggattaagat
     1801 ggttttctcc ggtgcggcac gcgaccgtgc agtggccgcc gcgccgctcc gtgatctcca
     1861 gaccgcagag gtcgttgaaa gggctgttct cggagaaaag cggcgcgggg gtttctgtct
     1921 cttttttcat ggctggctcc ttgaaattgt ttagcatgga aagattatac tccgaacggc
     1981 gcggaatgaa aagaggagag ttgcgccgga gaaaagccga tggtataatg ccggaaaaaa
     2041 gaggtgaatg tcatggtaaa aaacgttgcg gtcgtgagcc tttccgccgg tgtactgggg
     2101 gagagctttg caaagcatga gctggatatc ggcgtaaagc gccttgcgga ctatgggctc
     2161 aacgtccggt tcatgccgca cgcgctcgcc ggtatcgagt atataaagaa ccatccggag
     2221 gatcgcgcgg cggaccttct cgcggcgttc cgcgacccgg agatcgatat gatcctctgc
     2281 gccatcggcg gggacgatac ctaccgcctt gcgccgtacc tctttgcaaa cggcgaactc
     2341 gagaccgccg tttccggcac ggacaagatc tttctcggct tttcggatac gaccgtcaac
     2401 catatgatgc tccacaaggt gggcgtaaaa acgttttacg gccagtcttt cctctcggac
     2461 gtgtgcgagc tgggcggcga gatgctgccg tatacgcgcc ggtattttga agagctcatc
     2521 caaaccggca cgatccgcgc cgtcacgccg agcgatacat ggtacgaagc gcgcacggac
     2581 ttttcaccgg agcaggccgg cgtgccgctc ccggcgcatc cggataccgg ctttgagctt
     2641 ttgcagggaa gagcccgctt ctccggtaag atcctcggcg ggtgcatcga tacgctcttc
     2701 gacttcttcg acggcgggcg gtatgccgat atgccggtgc tgtgtcaaaa gtacggcctc
     2761 ttcccaagcg cggaggactg gaaggggagg atccttctga tcgaatcgag tgaggaatat
     2821 atgccgccgg agaagtaccg gcgcgcgctt gaatatttca agaccgccgg ggtattcggc
     2881 gcggtgtccg gcgtgctcgt cggaaagccg atggaccgga aatacgaggc agaatataaa
     2941 gccgcgctca cggatgtgat cgccgatccc acgctgcccg ttgtctgcaa catcaacatc
     3001 ggccacgcgc tgccgcgctg catcctcccg ttcggcgtgg aggccgcggt ggatgtgaac
     3061 gaacagaaga tcacctttgg ctgaatacca aaagcccgga ctgtgaatgc ttcacagccc
     3121 g
//
LOCUS       DAWAAE010000188         3053 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11731_length_3053_cov_4.122415, whole genome shotgun sequence.
ACCESSION   DAWAAE010000188 DAWAAE010000000
VERSION     DAWAAE010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_3053_cov_4.122415"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          919 c          962 g          671 t
ORIGIN      
        1 ggctggggcc gtcgggtcca tccggatcgt cgggcgcgga ggacggatcc ggagattttt
       61 ccggattgtc gggcgcagaa gacggatccg gcgatcggtc cggctcatcc ggcggcgcgg
      121 aagggctggg ctcgccggaa aggtcatccg acggcatctc ggtatcggag tcggcgccga
      181 gaccggcggc gcggccggta gacagcgcct cgggccggag cagcgccgta ttctcttcgc
      241 gcggatcggg cggctcgacg cgctgcgtgc cgctcacggc gaacacgcag ccgagcagca
      301 gcgccgccgc cagcgcaagg ccgagcgcac gggagatcgt ttgattgttc acattcacgt
      361 ttcctttctc cgtctccccg cgcggcgttc ttcgtccgcc ggggagcggt aaggccgggt
      421 catcagttcc agcgtgcgta aagcgtcatg ttcttatcga ccgtgtcttc gtcgaagtcc
      481 cacggctgcg tgcattcttc gtccttgaac cagccgccga agagcgagcc ggtcttgagc
      541 ggcgtggcgg gcatttcgat ctttgcgccg cgccagacgc gcacatcgtc aagctccgtg
      601 ccgccgaagg tcacaaactg cacacgcttg cgcagcagga gaaggaggat cagcagcaga
      661 ccgatcaggc cgagcagcgg cagcagccac gcaagcagat gtccgccggt cttctccgac
      721 gcgtcggtat caagcggcgc gtcgctccaa ccggcgtaga gggtcatgtt gccctcaacg
      781 gtgtcgttgt cgaagtccca ggggtgggtg cgttcgatat cggtgaacca tccggcaaag
      841 tattttccct cgcgcgtcgg atcgtccgga cgggggatct tctcgccgta gggcacgcgg
      901 atctcatcca cctggctgcc gccgttgggg tcaaagcgga tgatgcactc gccggagagg
      961 atgaccgtaa cgtcgtcctt ggtggaggcg tagtgatccg cgccgtccct ggtggagaca
     1021 taatttgctt tgctgcggat atgggtcacg cccgtctcgg caatgacgcg cagctgaaag
     1081 ctgccgagcg tcgtggtggc gggccattgc gtgccgccgc cgagagagag gaagttcata
     1141 taaaaccgat gcatcccgtc aacgagagcg acctgcgact tatcgatgcc cggcatgagc
     1201 agcgcgctgc cctcgacgaa ctcaaagaat gcgggatcgt attcgatctc gttctgcacg
     1261 ccgtagaccg tgtaattctg ggcgctgtcg gtgcgttcca gggcgaagac aacggtgatc
     1321 acatcgccgc ggagaacgtt tttcgtgtgc tcgccgtcta cggtgagatc aaagtggaac
     1381 tgcatcgatt cgtccgttgc cagcgcgccg acggagcacc agagcgccag cagcagcgcg
     1441 agaagaagtg cggaaaggcg tttcaatgtt ttcatgggat ctcctctcgg gataaaggat
     1501 tttgttcgga aactcacctg cgccgccggg tcccggctgc aggggtcagt atccgggggg
     1561 atccccgcgg gcctcccccg gatgggggag gcttgcgggg atggggaaaa ttactgctgg
     1621 ttgattttgc tgatgaccgc caccgcgtcg ctgacgttca ggagcgtcga gccttccggc
     1681 gtgtcgtccg caatgtccgc gcagaggaac ttgtacatcg ccacatcgct gaaggacgca
     1741 tagtgcgcgt tgtacatatc gtacacgagc tgcgcgtcgt tgatgtctat gacgcccgat
     1801 ccgttcacgt caccgccgta gctgaccgtt ccggccgctt cgcccgccgt gatgctgagc
     1861 tgcgctgcgg cgtcttccac ggagagcgtc ggcgcgatca cgagatacgc atacgttccg
     1921 ttcgtgccgt acttcgtggt cttgaacatt ctctctccgc cgtaggtgta cacattgttc
     1981 gcgccgggat ccgcttccac ggtcacgagc cagacggact gctggttctc cagcttcacg
     2041 tacttctgca ccttcacgcc ctgcgtgctg accgcctttt cgaccgtcac ggtgatcgta
     2101 ccggagacga ccttggcgcc ggggatcgtg tagctgccat tcgtgccggt gagcacctca
     2161 tcgccgagct tcacagtgta gttgtacttc gcgtcctcgt tcacggtgaa cgtgaagtcc
     2221 gcgccggtcg tcgcggtctg cgggttgccg ccggtgacgt ccgccgcgcc gctgccggtg
     2281 aagttgaccg tagtcgtctc cgccaagccc ttttccgtct gcgtgaaggt gatgtggatg
     2341 tttccggtga tagcgtcgcc cttaatggta tacaaataag agtttataaa tgcagagaga
     2401 tcttcaaatt ccggcgtata cggtttcccg ttgatcgtca tcgtcagcgt gtaatcgtat
     2461 ttgtccgttt caagctcctc cgccgtaaaa acataatccg caccgtaagg aacggtatcc
     2521 gcaacgcgaa tacagccctt ggcgttgccg tccacggtga tcgggtactg cttcgccgtt
     2581 ctcttgccgg tcacggtgag atcgccggtg acgttcttga cggtataggt gccgtcgccg
     2641 ttgtcgatga cggcaacgtt ctcgtcgttc accgtcgcct tgacgtcggt gtattcgtag
     2701 tggctctcgt ccttatccgt cttggagaag gtgtagtccg cgccgtcctc cacggtcgtt
     2761 ttgccctcaa agatcttggg cagagtgacg ctgtgcgtcg ccttgatcgt gagcaccgtt
     2821 tccgcatcca gaagcttcgc ttccggcgcg tccttcatgg tggcgttcgc cgccttatcg
     2881 atcttcgcgc tcgtcacagc cacggtcgcg gtgcccgtcg ccgtggtctt gaccttgaag
     2941 ttcagcgtga acgcctcgcc gaggtttttc gcctcgccgt aggcctgcac atggaccttg
     3001 ccggccgttg ccgcgtctac gatgtagttt gtcgtgtcgc tgatgccggt gta
//
LOCUS       DAWAAE010000189         3047 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11756_length_3047_cov_2.755682, whole genome shotgun sequence.
ACCESSION   DAWAAE010000189 DAWAAE010000000
VERSION     DAWAAE010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11756_length_3047_cov_2.755682"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          643 a          872 c          962 g          570 t
ORIGIN      
        1 gcgctctatc ccttcggctc ccctgcgccc gcggcggagt ataccgagcg ggaataccga
       61 agcgcactcg ggcagagcgt gaatcttgtc ctgtgggaga atgggaggct ggagatctgg
      121 tatctctccg aagacggcga aatgttcgcg gcgcttcgca tgtacgagtt tgaccggccg
      181 acggcgcgga aaatgcgcgc gctcggcgtg gaaaatgcag aggagcttgc ggaatttctt
      241 gccgatcaca tcgatttttc cgcggtctgc gaaaataacg gcgcagcgcg ctctcttgtg
      301 gaaagagcgc gtgtatcgta tgatccggac gcggcggcgc ggctggaagc gttttataag
      361 agctgcgctt tccgcgcggc acgggagttt cagggcttct ttacggagaa tttttacggc
      421 gcgtgcttca ccggcgtata cggccagagc ggttacgagg acatcagcgc aaagctctcg
      481 gagcttgcgg agcggtacgg ccttcagtat gccagtgcta aagcgaagga agagaacaag
      541 gtcatctacg ataacggcgc gttctatgag gaacggcccc agccggaggc agtgggcagc
      601 ggaaaatggt tcgtccatta catcccaaag accgccctgt acacccgcat gacgcactac
      661 acggatttcg ccgagtatcg gagagtgtgg aactgcgaaa cggcatcggg gcagagcatt
      721 gtgtgcgcga cggacgggcc ggagaagatc agcggaagct acctctttta tgaaacggac
      781 agcgcctatg ttcttgtcaa cgtcggcgtg agcgacgcct cgcttatgga gaaagctgcc
      841 gagacgatcg actggacggc gtttgataag gaaggagacg ggaaatgaaa cggatcatcg
      901 tttttctgct gctggcggcg ctgctgctcg gcggctgcgg cacgcggacg ccggagacag
      961 aagcgcgcga agcgcagagc acaagtaccg ccgcgccggg cacggagacg cgcgactggt
     1021 cggagccgaa cgcggtggat gacggctact ctttccagca cgatccggag gcatactatg
     1081 aggagaccgg cgacgattcc ggcctctccg gtctgataga gaagaccctt ctcccggaat
     1141 acgtagaccg ctgggtggac ggcggagagg ttttgtggac ggagtttcat ggcctgagca
     1201 gcggcggaaa ccagagcggc acttattacg gctggatcgc ctttaccggt atgccgaaaa
     1261 ccgaactcgg ctggcgcacg gcggacttca acgggactaa atgctactgc cgcagtatct
     1321 ttatccgtga actgcccaac ggggacaata catacaccat tgttgcggcc aaaagctggc
     1381 ccatggacgt tgtccttgtc aacgataccg tcgccgccat tgggatcgaa gacctgtttt
     1441 ccggccgcgc ccttccgacg ctcgcgagcg cggagctgta cgttccgcag ctcggaaaaa
     1501 gctttgttat gacggaggag acggcgctcc gctcgctcga aaaggcgctc tccaaagagc
     1561 cggacaccgt gcgcatcacc gccacgggag cagatttcgg cgaacaggaa accctctgcc
     1621 cgctctacct gcgctttgcc gatgggagac gcgcactgct cttcaccgcc ggggacggct
     1681 gtgccgatgg gtcgctgctg ccatgcgcgg tgttcggcgg agagagcgta ttcgagcgct
     1741 tcggcgtgcc gatggacgcg gcaggctata cccataacac cgacggcact accaccgtga
     1801 ccaattcggt gggcggcggt gcttccgacg gcatgacgag cacggcacgc tacgacgtcc
     1861 agggacggtt ggtgtattgg gaacgggctt ggacgtgggc caatcgggac gatacggatg
     1921 tccatatcga gacggtttcc taccgcgagg acggaaaaat cgcttccaca ttgggccgct
     1981 cgcatttcgg ccagagcgac gaatatacaa cgctcacaac gtatgtctac aatgagcgcg
     2041 gcgatctcac gcgcatcaac gtggaatatg atccgacgga tgttttcggc cccggatact
     2101 accacgaact gcgctacgat gagcagaacc gcctcgttgc ctatgtgttc cactatgccg
     2161 acggcagcga aggacgccca agcggcaacg tctatttctg gtacacggag gacggccagc
     2221 gttgctacta ccatgtggac acgaacggca acctcgtcgg cgggccggac ggtgcctcgg
     2281 gcgacaaccc tctccgcaag taaagcggac agctctaaaa ctaaaagaca gagcctgcgg
     2341 catccccatg ggccgcaggc tctgtttctg tcatttattc ttcttccagt gcttcgcgta
     2401 cgggctgcca cgcgttgatg agcgcatcga gtgagcgcgc ggcgtttgcg gggctcgtgg
     2461 agggaaggac ggcgtcctcc cgcccggtga gcgggtagat atacttccgg tacagccggt
     2521 gggccgttcc gccgttcgtg aagatctgcc ggatgcatgc cgtttccaga atgggcgtga
     2581 ggtcgttcgg cacggcattc cggatggagg cgtcggacga acccgcaata tcgcacgagg
     2641 cgatcacatc ccagagcgca atgtggttcg ccagaagaaa cgcgcgcttt tcctccaccg
     2701 tctgcggcac gtccgcgcca agcagcgccg ccattacccg ccagaagcgg ttttgcggat
     2761 gcccgtaaaa gaacatctgc tcgcgggact taaccgaggg aaacgagccg aggatcagaa
     2821 tgcgcgaatc cagcgcatag atcgccggga agggatggcg cagcgattcc atttccattt
     2881 taatcacctc cggcgcggca attatccata agagtatata accgcaccgg cgcctttgca
     2941 agagaaatgc tttagccgtg cccctcagcg gcgatcatgc gcatggcgcg gctcggcgct
     3001 tccggccttc gaactgaaaa ccgaggtccg caacggcagt ttccagt
//
LOCUS       DAWAAE010000190         3036 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11807_length_3036_cov_2.340154, whole genome shotgun sequence.
ACCESSION   DAWAAE010000190 DAWAAE010000000
VERSION     DAWAAE010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_3036_cov_2.340154"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          601 a          950 c          858 g          627 t
ORIGIN      
        1 gttctcacca agccgtaaac gaaagctaaa aatccataca ggcgggcagg cacaagctgt
       61 gtctgcccgt cttgagcgtt tcaaatatgc aaaaaccagc acttcctgac ggaggtgctg
      121 gttttttggt gccggtggcg ggggtcgaac ccgcacgtcc ttgcggacac gggattttga
      181 gtcccgggcg tctgccaatt ccaccacacc ggcgtatggc ggcattatac cgcggaaatg
      241 tgccgttggc aagaaaaaat attccgcttc cggtattcca tttttagcgt cgatatggta
      301 atatagtttt gcctatttat gcactgaaaa aacggagata gactatgaaa cgatttgtcc
      361 ggccgggggc ttttctgctg cttgctgcgc tgcttttctg tctgagcggc tgctattccg
      421 gcaacatcga tcaatatttc tctcttccga tggcctcgga ggaataccgt cagctccaat
      481 cgctcatcga tgaggagctt gcctccggca gcgaatatgc cgcgccgatc cacggcagct
      541 accgccagag cgtgcagctc agcgacgtgg acggcgacgg tgccgacgag gcgctcgcct
      601 tcttccgcga cgctgcgaag aaccccaaga tcaacatcta cgattccgaa aacggcgaac
      661 accggctcgc gctctccatc tccggcgaag gctcgtccat cgggcggatc gactatgccg
      721 atctcaccgg cgacggacag aaggagcttc tcgtctcatg gcagatctcc tcgggtctgt
      781 ccgttctggg cgtatattct ctcgcgggct ggtccggcga cgtgcttttg agcaccgaca
      841 gcgccgagtt cgtcaccggc gacatcaacc gcgacggctg cgacgagctt ttgaatctgc
      901 gcgcggcgaa cagcacgacc tatcttgtcg atcaatacac cttcccgctc gggcaggaac
      961 cgcaggcgac ctcggcggcg ctcagcgccg gtatctccgc gctgcagcgg ctgcgcatgg
     1021 tgacgcttgc gggagacgcg cccgcgctgc tcgcggagtc tacgctcacg accggcgatc
     1081 ttgtgtccga tctcttcgtc tgccgggacg gttctctcat caatctcacc atgaaccgca
     1141 ccacgcagat ctccgaaacg cgccgctcct acagtcttgt atacgcgcag gacatcgacg
     1201 gcgacggctc caccgagatc ccgcacccgc agcagctcta cagccgcggg gacgaggttt
     1261 tctggtcgat cgcctggttc cgctacgacg cttcgggccg cgcgagcacg atcatgacaa
     1321 cctaccactg cgttacggac aactggtatc ttgtgctgcc gtccggctgg gacgtcgggc
     1381 tcacggtgcg gcgggacgac agtgtttccg gcgagcgcgc ggtcattctc tcgcggctgc
     1441 gctcggacgg ctcgcttgag gactatgttg ccgtatacgc catcaccggc gaaaaccggt
     1501 acgagcgcgc caggctcgat aaccgcttcc ttttgcagga ggaaggcacg acggtgtacg
     1561 ccgccaaaat tctcgacggc aagaccgacg agaacacgat ccgcagccga tttcatgtga
     1621 tttatacaga ctggagctcc ggctccgtcg ttggagggta agaaaccgat atgaagaaag
     1681 ttctggttct ggaagacgag gccaacatcc gaagttttgt cgttatcaat ctgcgccgga
     1741 gcggctatga gccgatcgag gccgactgcg gcgaaatggc gcttgagcag ctccgccgcc
     1801 acccggacat ccgcgtggcg ctgctggacg ttatgctgcc ggatatcgac ggctttgagg
     1861 tctgccgccg tatccgcgcg tccaacacgc gcatcggcat catcatgctc accgcccgca
     1921 gccaggaaat ggacaaggtc accggtctta tgaccggcgc ggacgactat gtcaccaagc
     1981 ccttctcccc cgccgagctg accgcccgca tcgacgcgct cttccgccag ggaccgttcc
     2041 gtctcaatac gcgcaaccgc acgctggaaa aaaacggcga gcgcgtgcgt ctgacgcaga
     2101 tcgagttttc catcatcaaa tactttatgg acaatcccgg caaggcactc tcccgcgagg
     2161 atatcctcaa cgaggtatgg ggccgcgact acttcggcga gctgaagatc gtggacgtga
     2221 atatccggcg tcttcgcatc aagatcgagg acgatcccac catgcccgct tacatcacca
     2281 ccgtctgggg ctacggctac aagtggggac tttaagactc ttcggaggat ctgcattcca
     2341 tgaagaagct gctcgctgac cttttcgcaa aaaaagaaaa aagccgccac gccgcagccg
     2401 ccaaaaaacc ggtgcgcctg ccgcgcctgc gcgatatcca tttccgctcc atccgcgccc
     2461 gcggtatgtt cgttggaagc ggtcttgtgc tgctggcgat cattattgcg ctagcagcgt
     2521 atactctctc gctgcaggcg tattactatt ccgccacgcg cgcctcgctg caggcaaagg
     2581 cggagaccgc ttcggcgttt ctttcgtcct atgtggaccg cacctacgcg gagttttatc
     2641 agcgcgcctt caactatgcc gaggattttg acgacaagga ttcgcttgaa ttgcagttcg
     2701 tcaacaccga gggacgcgta gagatcgcct ccaccggcat ttccgccggt tcgatccccg
     2761 gctcagatga tatcaaaaac gcgctcgact ccggcaggat cagcttctgg cagggcaagc
     2821 gcgcctccac gggggaaaag gttctcgccg tatccgcgcc gctcgtttcc ggcagcggga
     2881 gcgtcgtcgg cgtaatgcgg tatatcacaa gtctcaagct catcgaccgc gcggtgaaca
     2941 cctcctcgct cgtcgcgggc ggcatcgggc tcgtggtgct cgcgatcgtt gtattctcga
     3001 acctttactt tctgcgcacg atcaccgaac cgatcg
//
LOCUS       DAWAAE010000191         2996 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_11974_length_2996_cov_2.630398, whole genome shotgun sequence.
ACCESSION   DAWAAE010000191 DAWAAE010000000
VERSION     DAWAAE010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_2996_cov_2.630398"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    465..474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          883 c          892 g          665 t
ORIGIN      
        1 ccatagaaca ccgcccgatt gttgaacgca cagggcaggg attttgcctc ttcgagcgtg
       61 tccacatgga cgaaccgcgc ggcgagccgc gcgcgctttg cctccacgcc cggatgcgcc
      121 gttttcaggc ggatgacgca gcaaaggtgc tcggagccgc tattctccgg cgtcagattt
      181 acaaattctc cgtccatacc gctgctctgt tcagccgaga tgcttctgga agaagctcac
      241 gtccagatat tttccgaatt tgaaggcgac agcggacatc acgccgatct ccgtgaagcc
      301 attcttgcgg tggaagcgca gcgagccttc gttgtcggac gtgatgatgg agatgagatt
      361 ttctatgccg cgctcgcgtc ccagccgttc gatctccgca tagagcttct gcccgacgcc
      421 gccgccgcga caggaatgag ccacgtaaat ggaaagatcg gcggnnnnnn nnnntgcagc
      481 ggtaggcgct gcgggcattg aatacgtcga gataggcgta cccgatgggc tttcccgcgc
      541 cgtcgcgcgc gacgatgtac ggatagtcct tcgtgatgcg cgccacgcgc gcgccgaatt
      601 cctccggcgt gaccggctct tcttcgagcg tgatgcaggt gttttcaata tagtaattat
      661 aaagctccgt gcatgcggcg atatcctccg gcgcgatggg cgcgagcgtt accgccgcgc
      721 ggcgcgcggc gatggcagct tccagaacgg caatatctcg ctggtccttt tcgcggttga
      781 gctgccgctt gagcgcgagc acgtcctcga gcggcgcgac cgggatgccg ttcagaaaca
      841 ccgtctcacc cgggaacatg ttttcataga tctccacgtc cggcgcgggg acgaaccgcc
      901 gcgtgccgtc cgggagcacc tgcacatcgc agcgctgcgc gagcgtatcg ccaagctttt
      961 tcgtgcacca tatgtccaga tcgtgcgtct cctcgcgcag accgtgaagc accatcgcac
     1021 cgccggcatt gatgcggtat tcgttcgtgt cgagcgcgag ctccgcgagc cgccgccgaa
     1081 tgtcctctgc cttcatcgcc ttttcctctc tttttccgaa gaactttccc tcagaatgta
     1141 gcacacattt ccctgtgctt caatcggttt ttttgtacaa gagttttttg ggccaacgcc
     1201 ttgccaattc gacggagaac ccatataata tacacattat gaaaaaagga ttgagcttga
     1261 aaacggcgct gacggctctg gcggcgtcgt ttgtctgggg aaccgcgttt gcctttcagc
     1321 gcatggccgc aggacatatc ggagccatca cctttaactt ctatcgcagt gtgctcgcag
     1381 ctttgtcgct gggagttctt ctttttgtat ccaaccggcg gaaaaagccg gaggagcgga
     1441 agaagagcga cccgaaaaag atcgccatcg gcggcaccgt gtgcggcgtg cttgtgatcg
     1501 ccgcgtccat cgtgcagcaa tacgccattg cggacgttga agcgggcaag gcgggctttc
     1561 tgacgacgct gtatatcctg ctcgtgccgc tcttcagcgt ggtgttcctg cataagagag
     1621 ttgcgccgag tctctgggtc agcgtcttgc tgggactcac ggggctctat ttcatcagcg
     1681 tgaaggaagg cttcacgatc tccccgagcg acgcgctggt ccttatgagc gcggggatct
     1741 atgcggtgta cattctcgcg gcggattact atgtccgctt cggttcgccg acggagctca
     1801 actgcgcgca gttcatcgtg gcgtctgtcg tctgtctcat cggcatgttc gccgtggagg
     1861 aaccgacgct cgccgagctt cgcgacaaca tcatcccgat cttgtatctc ggcatcttct
     1921 cgagcgcgat cgcctatacg ctccagttcg ccgcgcagcg cgacggcaac cccgtcgccg
     1981 tgacgctgct tttgagcatg gagtcggtat tcagcgtact gggcgcggcg gtgctgctgc
     2041 acgagtttct gtccggacgc gagcttttcg gctgcggcat catgctctgc gccgttatcc
     2101 tcgtccagct tccggagggc ttctggaagc ggaagataaa aacgaataac gagtaactag
     2161 taacgaacat ccccgctccg gcaaagcgcc gaagcgggga tgtttttcgc attatgcaaa
     2221 gtccttacga atctgtgggc ttttcggtcg tcagaaggtc gatgtccgcc caacctgcga
     2281 agccgtcgcc cttgatgagg atcatgtatg ttccctcccg cgccagagcg gtcacttccg
     2341 taccgtcttc cagcagccgc gagacctcct ctcgggccag gtcgttcgtg atatagatcc
     2401 cggcgccggc ggtggatttc acatagtagg tctcataggc gtgcagcaag ccgtgcgaag
     2461 gtctttccac atccgggaag tccgcgagat ccttgtggcc ggggcagtaa agcgccgtcg
     2521 gcgcatcctc gagcatagag gcggtggccc agccgacgcg cccgtcgttt acgatgatca
     2581 gcgccgattc tccctgccgg gcaatgacac gcacctccgt ggcgtccagc acctgatcaa
     2641 aatgctgcgt ccggttcacg tcgctggaaa ggaaaatcgc gtacccgccg gaggatttta
     2701 catactgtac ctcgtattcg ggaaggtatt cttccggccg ctccacatag ccgaatccgt
     2761 ccagatcggc ggcgttcggc gtaggcttcg gctccggcgt tgcggtgggc tccggggtcg
     2821 gcgtgggtgt gggggccggt gtggaggtgg gctcggtggt cggcgcggga gaaggctccg
     2881 gcgcggcgct tccgcagccg cacagcgcaa aaagggttag tgcaagaagc agggcaagac
     2941 actttttcat cgtcatcgat ctcctcgtac ggaaaaattt gacggattta ttatat
//
LOCUS       DAWAAE010000192         2963 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12132_length_2963_cov_2.892022, whole genome shotgun sequence.
ACCESSION   DAWAAE010000192 DAWAAE010000000
VERSION     DAWAAE010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12132_length_2963_cov_2.892022"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          570 a          862 c          917 g          614 t
ORIGIN      
        1 tccctgtcta cagcgagggc gagggctggc agcgtcagct tcacatctac cagagcccgt
       61 tctactatat cgactactgc ctcgcgcaga ccgtcgcgct ggagatctgg gcgatgctgc
      121 agaaggaccg caaggaagcc tgggagcgct acatggcgta cacccgtcag ggcggcagcg
      181 aagtattcac aacgcttctc aaaaacgccg ggctcgaaag cccgtttgat gaaaactgcc
      241 tgcgcaccgt gtgcgaaacg gcgaagagct ggctggataa ttacgatctc accggcatcg
      301 aataatcccc gcgctgaacg aaaagccggc cgtgcgcgtc atatgaataa acgccggccg
      361 gcttttttaa ggatgactat atgaaatttt tccgatccaa actctggata ctgcttcttg
      421 ttctcgcgct cacggcgtcg atcggcgcgt gcggctaccg tcccgaaccg gagaaaacgg
      481 agagcggtca gacggtgttc cgcctcggct tttccgaaac gccgtcgtcg ctcgatccgt
      541 acaccgccga aacggatgcc gcagccgcgg cgatctcgct tttgtacgat acgctctttt
      601 atgccgatat cgagaccggg cggtacatca acagcgtctg tcaggaatac acggtgcagc
      661 ccgccgccgc ggacggggcc cggctctgga cgctgacgct gcggaacgac gtatattttc
      721 acgacggaac ggcgctcacg gcgcgggatg tggagttttc gctccagtcc atgaaggact
      781 tttcgctgct ctatggctat ccgggctgcg agctgatcga cacgaccggc atttcggtca
      841 cggacgatac gcacctctca ttcctcgcct ggggcgacgt tgcggcggtg gaggaatgcc
      901 tttcccgcgt gccgatcgtg ccgaagagcg tttggaacac gctgcccggt atggagtata
      961 cgacctccgg cattccgaag gacgcatcgc aggcgaagaa gagccttgcc gctcttgtcc
     1021 tgacgccgga gacgatgacc ggctcggggc tgtacatctg ggacaaatac gaaaacggcg
     1081 ttctgacgct gcgccgcagc gaggattact ggaacggcac cgcccgcgcc gagatcgtgg
     1141 agctgcattt cggctgtgac gatctggccg cggcgctgga gaacaatgcg atcgacgcgg
     1201 cgtggaatct ctcccgtgcc gccgcggagg agctggccga ggggcgcagc acggcgtacg
     1261 gcacgtccgg cgagctgctg ctcgtggagt tcaaccagcg cacggacgat acttccgggg
     1321 gaaacgccct gcttctcgat gagaccgtcc gcgaggctgt cacgctctgc gccgaccggg
     1381 agacgcttgc gctcaccggc ttcggcggtg cgatgcctgc ttacggattt tccgttcctt
     1441 acgggacgga aggctctgct gctgcggtat gcagcgcgga gagcgccgcg gcgcttcttg
     1501 aaagcgccgg atacacggac gcggacggcg acggcgtgcg cgaatcgccg gaaggggaga
     1561 gcctttcctt ccgcattctg tgcagcgatt cctcaaccgc gtgggtgcgc gcggccaatc
     1621 tcctgaaaga tatgtgcgcc ccggcgggta tcgcgctgga gatcaccgtc ctatcgccgg
     1681 acagggcgct cgccgcggcg gaggtgggag actttgatct cttcctgacc gcctcgcaga
     1741 gctactatga cgcgtatctc tcccttgcgt ccttttactg gaacgatggg aacggcgcgt
     1801 tctggaacgg cgcggcgtcg ccgggatgga acatttccgg ctacgcaaac agcgagtttg
     1861 acgcgctgta tgaagagctg acgtttgcct cctccgccgg gcgcgacgcg ctcgaacggg
     1921 agatggagga tatggtgctt accggctgcg cggcgctccc gatcgcgttt gcggcaaaga
     1981 atcaggcgtg cagcggcgca tggctcggcc tccgtgccga ccatggcgcg ggactgttct
     2041 ttactcccgc catcctccgc cagcagctgc agggcatgac aaacgggaga taagagaata
     2101 aaaaccagcg ccgcggaaca ttgcgttccg cggcgctgct gttgtatacg gcgtttatga
     2161 ttcagggctt gacaacaacg tcggcttttt cgagcgtttc tacgatggaa tacatgttcg
     2221 tgatgccgcc gacaaggagc ttctccttgc gatggtagta gtcaaggcac gtgccgcagg
     2281 tgagaatgtc cacgccctgt gcttcgagtg ttttgagatc ttcgatggac gggccatccg
     2341 tgctggtgac gaaagcgccg ctattataga agagaatcgt tttcggcagc gcgtcctgct
     2401 gcgtcagcgc gaaaatgaag gccttgagca gtgtcttgcc aagcgcttcc tcgccctcgc
     2461 ccatgtggtc tttggaaatg gcgactacga tgtttttctc cccctgcttg agaggcgcgc
     2521 atacgacggc ttcgtcggcc acgtcctttt tggcgccggc ggcgtcgatc gtgacagaaa
     2581 accgcgcttc gccctgcttt tcggcgcgga cggcgaagcc gtactgtccg gcgagacgga
     2641 tgagattctg tacggcggcg tcgttatcga cgagcacctg cacggtctcc ggcgcagtga
     2701 gcgcgtcgat cgcctttttc gtctttacga ccggcatcgg gcaggcttcg ccgagagcgt
     2761 ttacggtaat catataaaag acctcctgat cggtattttc tacgaagaga ataccacatt
     2821 tttcgcggta gatatattaa aaatatagat aaaatcggca gaatcataga ttctgccgat
     2881 tgagcttgtc aaaaagtatt tttgacaagc tctcaaacga gacccactgc gttgggctct
     2941 cgtttgaagg ggctgcggct cgc
//
LOCUS       DAWAAE010000193         2941 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12230_length_2941_cov_3.278586, whole genome shotgun sequence.
ACCESSION   DAWAAE010000193 DAWAAE010000000
VERSION     DAWAAE010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12230_length_2941_cov_3.278586"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          563 a          938 c          881 g          549 t
ORIGIN      
        1 gcgatatcga tgccgtgctc cgcgactgcg atattgtcat tgaccgcgtg taccacacga
       61 aagcctgcca gcagacgccg atggaaacgt tccgcacgtt ctgcacgatc gacacttacg
      121 gccggctgca catcgtgagc tccacgcaga tcgtgttcca cacgcgccgc atcgtggcga
      181 acgcgctgca catcccgcgc tcaaagatcc gcgtgagcaa gccccgnnnn nnnnnnttcg
      241 tcacatggat gacgaaaaag ccgagcatga tcgtcttctc ccgccgcgag tgcatgatcg
      301 cgggcagccc gcggcacgaa atggagatgc atgtgcgtct cggcgcggac aagaacggca
      361 ttgtccgcgg gatcgatctc tatacgctct ccaataccgg cgcttacggc gagcacggcc
      421 ccacgaccgt cgggctttcc ggccacaaat ccatcccgct ttatgccaag gccgaggcgt
      481 accggttcgt gtacgacgtt gtatacacca accacatgtc ctcgggcgcg taccgcggct
      541 acggcgcgac gcagggcctc ttcgccgtgg agtccgccgt gaacgagctg gccgcgcggc
      601 tcggcatcga accctttgag atacgaaagc gcaacatccc ccgtgagggg gagataatgc
      661 ccgcctacta cgggcaggag aatacgagct gcgcgctcga ggcgtgtctc gaaagcgtgc
      721 ggcggcgcat cggctgggac gaaaagtacc cctgccgcga tatggggaac ggcaaggtgc
      781 gcgccgtcgg catgggcatg gcgatgcagg gctccggcat caacaacgta gacgtcggca
      841 gcgcgacgct caagctgaac gacggcggcg ggtacaccct ctccatcggc gcggcggaca
      901 tgggcaccgg ctgcgatacg atcctcgcgc agatcgccgc cgaagcgctc gactgtccgc
      961 tggagaacat ttccgttctc ggcgcggata cggattcctc gccctacgat tccggctcct
     1021 acgcttccag cacaacgtac gttaccggca aagcagtgga gcagtgcgcg ctcgcgctgc
     1081 gcgaaaagat cacggcgctc gccgcgcgta tgctcgcgtg cagcgaggac gacgtgctcc
     1141 tcaccgggag ctgcgccgaa acggcggacg gggagaagtc cgtctcgctc ggggagatcg
     1201 cgctcgcctc ccagtgcgga aatgcgatcg cgctcgaagc gaccgtgacg cacacctcgc
     1261 cgatctcacc cccgccgttc atggtgggcg cggccgagat cgagcttgac cgcgagaccg
     1321 gcgaaacgca ggtcgtgaac ttcgtcgccg ccgtggactg cggcacgccc atcaacccga
     1381 atctcgcccg cgtgcaggcc gagggcggca tactgcaggg catcggcatg gcgctcacag
     1441 aaaacatcac ctacgaccgc cgcggcatgc cggtggaaaa ctccctcatg cagtataaga
     1501 tcccgacgcg gcaggacatc ggccacatct ccgtcgcgtt cgagtcgagc tacgaggaga
     1561 ccgggcccta cggcgcaaag tccatcggcg aggtcgtcat caacacgccg ctcccggcga
     1621 tcgccgacgc ggtgtacaac gccaccggca agcggtttcg tgagcttccc atcacgcccg
     1681 agcagatcgc catggcagcg aaataaacga aaactctctg ctgtttttgg cagagagttt
     1741 ttgatataaa gaaaaatgtc cgcgatagga aaacttttta ttaacagtac ggcgaaacta
     1801 tggtaaggta acactcgaat tccatagaag ctttcaggat tttcgaaaac tgaaagtgga
     1861 ggacactctg gagaatcccg tttgatcggg tgccgaaggt gcaaggctcg cattccgggc
     1921 cgaaactctc aggcaaaagg acagggcaga atcgtgatag accgcgaaca tgtacggggt
     1981 tatttccgaa actgcatatt ctctggagct gccgaaatcc ggcagctttt tttctattta
     2041 tctaaggagg atcccgcaca tgttcaccgc catcgaaaac gcgctctacc ccgtggtatg
     2101 cgatctcaac gcgtatctat ccaactatgt tctcgtcttt cttctcatcg ccgtcgggct
     2161 gtggtactcg atccgcacgc gctttgtgca ggtgcgctgc ttcggcgagg gtatgcgccg
     2221 cgtgttcggc aatcttacgc tccgcggcga gaagcacgcg agcggcatga gctcctttca
     2281 ggcgctcgcc accgccattg ccgcgcaggt cggcaccggc aacatcgtcg gcgcgtccgg
     2341 cgcgattttg accggcggcc ccggcgcgat tttctggatg tgggtcatcg cctttttcgg
     2401 catggcgacc atttatgccg aggcgacgct cgcgctgacc acccggcagg tcgataaaga
     2461 cggcacgatc cacggcggcc cggtctatta catcacgacc gccttcaagg gcggcttcgg
     2521 aaagttcctc gcgggctttt tcgccgtcgc tatcatcctt gcgctcggct tcatggggtg
     2581 catggtgcag tccaactcca tcggctccac gtttgagacc gcgttcggcg tcccggcgtg
     2641 gatcgtcggc attgcgctcg ttgtcctctg cggcgtcatc ttcctcggcg gcgtgcgccg
     2701 tctcgccgcc gtcgcggaaa agatcgtgcc ggtcatggcg ggaatcttcc tcgcgggcgc
     2761 gctcgttgtg ctcgttgtgc gcatccagta catccccgcg acgttcggca tgatctttaa
     2821 atatgccttc cagccgcagg ccatcctcgg cggcggcttc ggctacgcgc tgaaaacggc
     2881 gctctcgcag ggcgcgaagc gcggcctgtt ctccaacgag gccggtatgg gctccacgcc
     2941 c
//
LOCUS       DAWAAE010000194         2911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12380_length_2911_cov_2.601891, whole genome shotgun sequence.
ACCESSION   DAWAAE010000194 DAWAAE010000000
VERSION     DAWAAE010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12380_length_2911_cov_2.601891"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          587 a          911 c          796 g          617 t
ORIGIN      
        1 ccattggaag ctgaacccac ttcctcgcaa tttatcgaga taatctcttc ctacacaatt
       61 cgttctcagc agccgcagct tcagcctctc ggtctgccgt aatccgttgc tcgtttctct
      121 taaaacatac ggccgtcttc ttttccgcag gtatttctcc gacagccgca gtccgcgcga
      181 ataccgggaa acgcatccat aagccgtaat attccgctct gtcccctgaa ggcccgtggt
      241 tttaagcaca ggccttcatc tttttcccga aaaagtcagc gttgtgcatt gccgccagag
      301 atctccggcg cgcaggatgg gcttttcaac gccatcgcag gccatggcat tcggaaagta
      361 ctgcgtttcc aggcacaggg cgcagcgcgg cgcatatatt gcgccctgct tgccgcggcg
      421 ttcggtcagg aaatttgcgg tgtagagctg cacgcccggc agcgtcgtcc gcaccgtcat
      481 ggtgatgccg gacgcgtcgc cgcgcagtac cgccgccgtt tctcctgtca ggcagaagtt
      541 gtggtcgtac ccgccgccgt ttcgcagttg gatatcgtcc gcgccgatat cctgcccgac
      601 gcgcttcggt acacggaagt caaagggcgt tccggcgacc ggcagccgct tcccggtcgg
      661 cagagtcgcc gcgctgtttt ccagaaactc ttcggcgccg atctgcagcg tgtggccgag
      721 cacggaaccg ctgccgttaa gattaaaata gctgtgattc gtcagattgc aaagggtatc
      781 ccgatcggcg gacgcttcat acgccatcgt cagcgtatcc ccgcacaggc gatagctcac
      841 ggcggcgcac atcgtgccgg gatacccctc ttccccatcc ggcgatacgc gcgtgagccg
      901 tacgccgtcc ggcagcattt ccgcagacca tacatatttg tcaaagccgc gcacgccgcc
      961 gtgcagatgg ttctccccgt cgtttccggc gagccggtag tctttcccgt tcagcgtgaa
     1021 ggccgcgccg ccgatgcggt tgccgacgcg tccaatgcac gcgccgagat agccgtcgtt
     1081 ttcctcgtat tcccgaagcg tatcatagcc aaggactgcg tccgtccacg cgccgccatg
     1141 atgtacccga agcgagcgga tcgccgcgcc atactccatg atctccacgg ccagcgcttc
     1201 atttttgatg caatagacgc tgacagactc cccgtccttc gttacaccaa aaggcttctt
     1261 ttccaccgag accgacatgg tattttctcc tttcacgccg cagcgccgct tccgtttctt
     1321 ctcctgtccg ctcagctgcg ctcgcatcaa atcagcttca aaagccgttt ctccggaact
     1381 tccggcagaa cgccgcgcag atgggcgaga atgcgctctc tcgatccctc cggctttctg
     1441 cggtaggccg aagtgacgaa gtccgggccg agctgcgtct ccggcgtcgt atactccgct
     1501 atgccccagc cgtagggccg cctgaattta tcctgcgaat acacaaagtc cccgatggta
     1561 ataaaggtct gcatctggag acgggtgatc accgtatcga agcccgtgtt gccgcccttg
     1621 cggtaatttg cgtgtatata cctttccaga ttcagaatac cacgccggag agcttggcac
     1681 aagagacgag tctcttgatt tgtccgctgt ccggatgcta caatggatat attccgcaaa
     1741 ggatcaaaaa aaggggagca gacatggaga agatcaaaaa gaacttcggc tttggcttta
     1801 tgcgtctgcc gacgaaggac ggcgagatcg acaccgagga gaccaagcgc atggtggatg
     1861 cgtttctgga cgccggattc aattattttg acaccgccca cggctatcac aaggagaaga
     1921 gcgagatcgc gctgcgcgag tgtcttacga gccgctatcc gcgcgagagc tacgttctcg
     1981 cggacaagct gaccgaaaat tattttaaga ccgaggcaga cattcgtccc gtattggaag
     2041 agcagctcgc cgcctgcggc gtggagtatt tcgatttcta tctgatgcac tcgcagtgca
     2101 cgctcaatta tccgcacttc aaggcgtgcc gcgcgtatga gaccgcattc gcgctcaagg
     2161 ccgagggtaa ggtgcgtcac gtcggcattt cgttccacga ccgcgccgag gtgctcgagc
     2221 agatcctgac cgagtatccg gagatcgagg tcgtgcagat ccagttcaac tacttggact
     2281 acgacgaccc cgcggtgcag agccgcaagt gctatgaggt ctgtgtgaag cacggcaaac
     2341 ccgtcatcgt catggagccg gtcaagggcg gcaatctcgt caatctcccg gaggaagcaa
     2401 aggccgttct cgatgaactg cacggcggca gtccggccag ctacgcgctt cggtttgccg
     2461 cagggttccc cggcatgatg atggtgctct ccggcatgag cagcatggag cagatgcgcg
     2521 acaatctctc gttcatggca gacttccggc cgctcaacga taccgagctc gccgccatca
     2581 cgcgcgtgca ggccattttc cgcggcaagc atctcatccc ctgcacctcc tgccgctact
     2641 gcacggacgg ctgcccgaag cacatcgcca ttcccgacct gttcgccacg atgaacaccc
     2701 ggcagctcta ccgcgactgg aacgccggtt actactatga tatccacacc atggacgggc
     2761 gcaaggcgtc cgactgcgtc aagtgcggca aatgcgagca cgtctgcccg cagcatctgc
     2821 ccatccgcaa gctgctggag gatgtagcga aggagtttga aacgaaagaa tgatcgaagc
     2881 atatctcccc cgtcccgatg agctgtggtt c
//
LOCUS       DAWAAE010000195         2886 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12503_length_2886_cov_2.569763, whole genome shotgun sequence.
ACCESSION   DAWAAE010000195 DAWAAE010000000
VERSION     DAWAAE010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12503_length_2886_cov_2.569763"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    337..346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2623..2650
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          520 a          859 c          967 g          502 t
ORIGIN      
        1 cggagctgga agcggaaatg gcagcggtga acgaaaagct cgccgctatc gacaaaactg
       61 ccgaggagct tgcgcgcgcg gaggaactgg cggaaaacgc ccggctccac agcgcaaatg
      121 cccagaaaaa tttcgccgga gtgcaggaaa agcggcgcgc cgatgcgatg gcgcagctcg
      181 aagcctgccg tgcggcgcgt gaaaaggccg taagcgaggc cgcgcggcgt gagcaggcgt
      241 ctgccacggc agacgaggcg cttcgcgccg ggatcctccg cggcgcagag ccggacgaaa
      301 cggcggaaaa ccgagccttt gacgacgaac ggcgggnnnn nnnnnncccg gcataggacg
      361 gtgggtatgg atactgcttg tgcttcttgc cggcggcttc gctgccgcgg gctttttccg
      421 cagcgtggtt tggatcggtg ctgccgcggc gcttctcgcg ctcgcggcgg tggggatcgt
      481 atcggttttc tgttcgcgtg accgtgccga tgccaaccgg gtgctccatg agatactgga
      541 ccggtacggc gccgaagcgc cggaggatat tccggaacgg ttcgacgagt attacgcgca
      601 atgggaagag gccgacgccc tcgcccgtgc ggcggaggaa gagcgcgaat acgcccggag
      661 ccttgccgaa aaggagctgc ggctccatca ggaactcacg gcgatcctcg gcggcgacgc
      721 cgcgccgctc caccggatgt cggaaaacga aaagaaggag cagcttctcc gtgaggagac
      781 ggcccgcctg cgcggacggc ttgatacgct tggcgatccg ctggtctgga aaacccgcct
      841 gcgtgagctg gaagcggaac acgaacgtgt ggaatttgag ctggaggctc tgaccgccgc
      901 gctcgacgag ctggcgcttg ccgacgaaga gatgcgctcg gagttttccc cggcgctggc
      961 aaaaagcgcc gcgtcggtgc tctcccggct gaccggcgga gcgtgtacgg agctcacgct
     1021 cgaccgcgag ctgaatgcgt ttgtccgctg cgccggggaa ccggtgctcc atgagagcgc
     1081 cttcctcagc cgagggacag ccgatcagct gtatctggcg ctgcggctcg cgctgtgcga
     1141 tatgctcctc gacggagagg atccctgccc gatcgtgctt gatgacgcgc tcatcaactt
     1201 tgacgatgcg cgcatgggct atgcgctcga ctatctcgcc gagatagcgc agcaccggca
     1261 gatactgctc ttttcctgcc acagccgtga aaagcggtat atacaagcaa aaaatgagga
     1321 ctgatgcgga atgcatcagt cctcattttc tgtatcggtt ttttcttcgg tttccacggc
     1381 gctgtcggta atgacgcgga tcacatcgtt ccgcacggag gcaaaaccgc cgtgcaccac
     1441 ggtgtgaaac acggcctcgc cgtgcagcag cgcgtccgcc gggccgtcgc ccagcgcgag
     1501 caccgccgga agatggccgc ggtgaaagcc gacctgtccg cccccgtgtc cggtctcatc
     1561 atcgcagacc gtgaggtgta cgctgtcaca gtgaacgtca acggcctcgc cgtgcggcgt
     1621 gaggacaatg agctcaagcg gtgcggtgga atcgtttgtt tttccggtca tgccgttatc
     1681 ccaccctttc agcggccctt ggcgtacacg tcctcaatgg aacctgccat gaggaagttc
     1741 tgctccggga tgttgtcgca atcgccgccg agaatggctt caaagccctt gatcgtgtcc
     1801 tcaatgtgca cgaacttgcc gggcatgccg gtgaagctct ccgccacatg gaacggctgg
     1861 gacataaagc gctgcacgcg gcgcgcgcgg gaaacggttt tccgatcctc cggggagagc
     1921 tcgtccatgc cgagaatggc aatgatatcc tgcagctcat tgtagcgctg cagtacgcgc
     1981 tgcacttcct tggcggtctg gtaatgccgt ttgccgacga tgtcgggcgt gagcatgcgc
     2041 gacgaggatt ccagcggcgc gacggcgggg tagatgccga gctcgacgat gctgcgcgaa
     2101 aggacggtcg tggcgtccag atgggagaac gcagtcgccg gggcggggtc ggtgaggtcg
     2161 tccgcgggga cgtagatcgc ctgcaccgag gtgatcgagc cgttcttcgt ggaaacgata
     2221 cgctcctgca gcatgcccat ttcagaggcc agcgtcggct gatagccgac ggcggaaggc
     2281 atacgcccga gcagcgcgga gacctcactg cccgcctgcg taaagcggaa gatgttatcg
     2341 atgaagagca gcacgtcctt gtgggagtgc tcgcggaagt attcggcgat cgtgagcccg
     2401 gcgagcggaa cgcgcagacg cgcgcccgcg gtctcgttca tctggccgaa gacgagcgcg
     2461 gtcttgtcga taacgccgga ctccttcatt tcgttccaga ggtcgttgcc ctcacgggag
     2521 cgctcgccga caccggcgaa cacggagtac ccgttgtgct caaaggcgat gtttcggatc
     2581 agctcttcga tgaggacggt cttgccgacg ccggcgccgc cgnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn accttgatgc cggtttccag cacctccccg gcggggacaa cgtcgacaaa
     2701 gtccggcgcg cggtggtgga tcggccagcg gtcggcggcg gggatcgggc cggcgcggtc
     2761 gatcggctcg ccggtgacgt tgatcatgcg gccaagcatt ccctcgctcg ccggaacgga
     2821 gatcggcgcg ccggtatcca cggcttccat gccgcgggac atgccgtccg tcgggcgcat
     2881 ggaaat
//
LOCUS       DAWAAE010000196         2862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12603_length_2862_cov_3.773424, whole genome shotgun sequence.
ACCESSION   DAWAAE010000196 DAWAAE010000000
VERSION     DAWAAE010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_2862_cov_3.773424"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          853 c          823 g          663 t
ORIGIN      
        1 tccagacgta gcccttctgc acctgctcct ccgtcacgat gccgtctccg ccggatacgt
       61 tcgcggcggt gggcgcagcg ggcgcgtccg ccgaggtggg cgtctgggtc atcgcatccg
      121 ttccgccgga gccggaagga acggcctcgc cgccgaccac atccggcatg gcgctgccgg
      181 actccacgag cgggagatac cagtcgtcat agtaataggg gacgtcgtcg ggatagtcgg
      241 cggcgaagtc atcccagagc tgcccccacg ggcgcagata aacctcgtag taaaactcgt
      301 cctcgccgtc ttcgtaccag ccctcaatgc agatcatgtt ttcaacatcg gggaagcggc
      361 tgttgatcgc gggatcaacg atccagtcgg catgctccaa aggcagattc gtgaagtagc
      421 cgctctccga catcacggta ccgtgctctc cgacaccggc gctgttcagc gtgaccgtcg
      481 cctgcgacat cggatcggct ctcgagtagt cctcgtccca gatcgtgacg gtgcccgtat
      541 agtccgagcc gatatcgatg acagcgcagg cgtcccacca ctttcccgcc atgctctcgt
      601 aagcgcccga gcagccggtc atcgtccacc agccatacca cgcgccgttc caccagtcaa
      661 gcagcgcatc acccgtcgga gcgggaaggg cggtgtctgt gctgcccgta gaatcggcgg
      721 acgtttcccg gctgagcgca gccgggtcga tggtgatcgt gccgtttttc ttgccgaaca
      781 gctgctcaaa cgtcactttt atcggggcag ccgcatcctt cagcacatag gccgtttgta
      841 tctcaagcgt ttcgcccggc gcgacatcct caaactgttt gtcaaccgcc gtagcgaagg
      901 aggcgtaatc ggtgataacg gcagcgacct ccagctcctc gccgttctgc acaagcgttt
      961 cattgatgct ccagatgtac gacgccgatt ccttgctgtt gtttgtaaag tccagcgtca
     1021 gaacaacggc atcgttcccg tcggaatcgg tcatgatgca cgcgtcctta tagaggagct
     1081 catagtcttc cagcttgatt tggttggact ctgtcttttt tccctcgccg ccgcagccgc
     1141 agagacttat tgccagcaca gcgatgagca gcaggctcaa aaagcgggtc atggttttgc
     1201 tcatgtcaat ctcctttacg tatccttcac aatgacattt ccgtatttgt ctgtgaactt
     1261 tcctgtggcg atcttgataa gatcgacgat cactccgatg ccgaaaaccc cggcggtaag
     1321 caggaacagt atgccggtgc cgatcttgcc gacatagaag cggtggatgc cgagcccgcc
     1381 gcagaagatg cagaggagca gcgtggtaag ccagctcttc ggcggctccg cgccgacggg
     1441 aactgcgggc ggaatataat ctgcgaccgg ctcgccgttg aggatcttct gtaccgcgtc
     1501 ggctacggcg tcaatgtagc cgccgcgatc tgtggcgaga ccgatcgttg cgcccttcac
     1561 gcccttgcgc agagcgctgt tcttgtcaac gcgcacgccg ccgactgacg ccttggcctc
     1621 cgaaatgacc ggctggacac ggatgtgcgt gccgttagag agataaagcc agagagtgac
     1681 atccagattc ggctcttttt caaacacgat gcgctgcccg gcgacgccgc ccttgatctg
     1741 gaacggcatc tggaacgcgg ttctgcgggc attgagtttg tcgaagatct cctcaagcgt
     1801 ggtggaatag gagagatcaa attcacgggg actggttgcg aatgctgcct ctttcatggt
     1861 gcctgcaaag tctgccataa ttttctcctt tctgaaaatc gacgtttggg gtgtgggccg
     1921 ttcttcttgc ttttgttcct tttgcgtttt acccgcgggc ggctcagccg tttaacgcgc
     1981 cgccgcagta ttcgcagcag ccgttggcat ccggcatggt cgtcgctccg cagaaggggc
     2041 aggtgaccgc cattttgggc gcggcggccg ccgcggcctc gtcgcggaca cgctgccgcg
     2101 cctcggtgag agtgtttttg atctcctgac ccatcgccgt atactccttg taatcgtggt
     2161 taaggatcgg gttcggcttg cgcacaagcg gcacgccgcc ggagggcgtc gtctcaacgc
     2221 ttgagctgtt cagcttgaaa cgcatttcgt caaaatacct gtggttcacg cggatcacaa
     2281 cataaaagtc atagctgaac gtatatcgcg ggggattata gcttacgcgc ttattctcct
     2341 tgtcggtggt gtattcctcg ctgcgttcct cgtcgatgtc gagatcgacg ccggtcacct
     2401 gcgagaagtc gatcacgtcg gggttggcgt cggtatagtt ccgcgcgcgc gtgacgatga
     2461 attttccggc gtcctcgtcg ataagcacct tcgtatcctc accgtagctg cgcgtcacat
     2521 ggagcgccgc aacggcattg cggttttcct cacggtaggc aagctgctcg cggatctcct
     2581 ccacggtgct gctgcgccgg tcggaaaacc agggtgagag cttcgcggag caatttttgc
     2641 agaggttgcc gtcctccagc ttacggttgc cgagaaagcc gatcttttcg ccgcagatgc
     2701 tgcaatattt cttgtcaaac agtcccatat gttttgatct cctttatgtt tgcttttcat
     2761 aatgataatg ctgttttttg tccgccccgg ggcggagcgc cggtcagtcc tgccgagcct
     2821 tctttttccg gacgagcagg agcactacga cggcggcgat ca
//
LOCUS       DAWAAE010000197         2851 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12662_length_2851_cov_2.929542, whole genome shotgun sequence.
ACCESSION   DAWAAE010000197 DAWAAE010000000
VERSION     DAWAAE010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12662_length_2851_cov_2.929542"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1614..1623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          814 c          781 g          653 t
ORIGIN      
        1 accggccttc ttgatgaagg accagccgcg ctcccacatg gagcggagca cgttggagag
       61 ggtcggccag tggtatgcag gcagctccat gacaaaggga gcgggctcgc cggagaacat
      121 cttggtcttc ttcagcatga taccggagac gatgatagcc gccatgccgc tgaagatggc
      181 acccgcgatc atggcgatga aggggacctt cgcgccgcag gggataaagg tggtggtcat
      241 gatcgtcata cggcggtcac gttcgttttc gatggtacgg gaagccataa cgccagggat
      301 gccgcagccg gtgccaatta acatcgggat gaaggactta ccggacagac cgaacttgcg
      361 gaagatacgg tccagaacga aggcgatacg agccatgtaa ccgcaggact cgaggaacgc
      421 cagcagcagg aacagaacca gcatctgggg aacgaagccg agcaccgcgc caacaccggc
      481 aacgataccg tcgttgatca ggccgctcag ccaatccgcc gcaccggccg cttccagcgc
      541 attgccgacg agaaccggca cgccgggcac ccacacgccg taatctgcgg gatcgggctc
      601 cgcaaagtcg gggttggtgt tctcgaagta atccacagcc tcaaggtagg tcatggggat
      661 ggtggattct tcgtccgcat tcgcgggaat ctcagagtag taaaccgtca tgtcgtcgac
      721 ggccagggtt tcttcatctt ccacttcaac cgtgccggaa gcttcggtcg tggtgaagga
      781 cttgatctct tccagagccg cagcggcgtc aaaatcttcc gccgagggat ccacgtccac
      841 aaacgcaccg agggcctcgg tagcgttggt gtattcttcg gcaacttctt catattgtgc
      901 ggagccgatg ccgaacagat gccagccgtc tccgaacagt ccatcgttgg cccagtcggt
      961 cgccgcggcg ccaacagtga ccattgcgat gtagtagacc aggaacatga ccagcgcgaa
     1021 gatgggcaga cccagccagc ggttggtcac aacgcggtcg atcttgtccg aaacggaaag
     1081 ctgcccggcg ctctttttct tgtagcagcc cttgatgatc tgagcaatgt aaacatagcg
     1141 ctcgctggtg atgatgctct cagcatcgtc gtccagttcg gtctcggctg tcttgatgtc
     1201 ctcttcgata tgccccatca cgtcagcggg aattttcaac tgctcaagca ccttgtcgtc
     1261 gcgctcaaag atcttgatcg cataccagcg ctgctgattc tcgggcaggt tgtgtacagc
     1321 cgcctcctcg atgtgggcaa tggtgtgctc aacgggacca gagaaggtat gcatcggcac
     1381 ggtcttggta ttcttggcag catcgatagc ggcctcggcg gcctccatga tgccggtgcc
     1441 cttcagagcg gagatctcca taaccttgca gcccagctca cgggacagct cgcctatgtt
     1501 gatcttatcg ccgttcttct tgacgatatc catcatgttg atggcgatga ccaccgggat
     1561 gccaagctcg gtcagctgcg tggtcaggta aaggttacgc tctaggttgg tgcnnnnnnn
     1621 nnngtcatgc tttttgagct tgccttcctt cttttccacc gtaacgccgg gccagttgcc
     1681 gacgaactgg ttagaacccg tcaatgcgtt gaacagcgtc gttttaccgc tgttcgggtt
     1741 gcccgccagg gcgattcgca gatccatgat gatttcctct ctttccaact cagaagcact
     1801 cggatagtgc caactaacca ttggctcaaa acaacgggac tttttgagtc ccgtgcttta
     1861 ttcgacctcg atcatttccg catccgcctt gcgcagcgaa agctcgtagc cgcgaaccgt
     1921 gacctcaatc gggtcgccca gcggcgccac cttgcggacg aatacgtcca cgcccttggt
     1981 aatgcccatg tccatgatgc ggcgcttgac ggcgccttcg ccatggagct tgacaacctt
     2041 gaccgtttca ccgatctttg cctgcttgag agtcttcatg ctctggtttc ctcctcctct
     2101 tgacttacct aaaaatcatc ctaccatgat tttacgggcc atttcctcac tgatcgcaac
     2161 gcgcgattcc ttgacgttga caatgatgtt gcctcccaaa gaggtgacaa ccgtcacgtt
     2221 tccgcccacg acaaacccca ggttttccag atgctggcga acctcctggc tgccgccgat
     2281 gcggcggatc ataacctctt cgtcgagatt cgctaagttc aaaggcatca tgcggtcttt
     2341 tttcctcctt cgtgaaactc agcgggatgc gctcgctgat tagcaggggc taacccttga
     2401 gctttaattt aggttagctc ttgctaaccg atttatataa tactcatgca ggcttttgct
     2461 gtcaaggatt tttcgcattt tcttgtgtta attattacta actatttcgc aggcgctttc
     2521 cgcttgtaaa tttcgtccgt actgctacaa tgaaggcaaa agccgcagta ttcctgtggc
     2581 aagctttaag gcggaggaaa acagcatgga atattccgtt gtcacgagag atttctccca
     2641 ttgccatctg ggcgttgacg gggacagcat cacgcagggc agccagtggt cggcggtagt
     2701 gagccggatc ctcggcgtgg cctcacatca caacgtaggc gtgggcagcg cggtgtggta
     2761 caagcgcacg ctggagaccg gaggcggctc cgtcaccacg cagaatttca ccgatcccga
     2821 ttttgcaggc atcagagacg gctggctgcc g
//
LOCUS       DAWAAE010000198         2822 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12796_length_2822_cov_3.159017, whole genome shotgun sequence.
ACCESSION   DAWAAE010000198 DAWAAE010000000
VERSION     DAWAAE010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_2822_cov_3.159017"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          568 a          798 c          867 g          589 t
ORIGIN      
        1 agtctgaccg tccggtctac tgcagcgatt gcttcgccaa gatcaaggcg aaccagtaat
       61 ttgcattttg ggtcatctga cacaggaaat gggagcggat catccgctcc cgttttcttt
      121 ttcccgattt cgtcgaaacc ggttgaaaaa taggaacagc atgggtataa tacgtctata
      181 tacttcttag gaggtcaaac aatgtaccag attgaaaaga ctactctgat cgccgagatc
      241 atggagaacg ctccgttcgt cgcgccgatg ttccaggcga tcggcatgca ctgcatgggc
      301 tgcgccatgg cgagcggcga gaacgtcgag gaagcctgcg ctgcgcacgg cgtcgatgtc
      361 gataagtttg tcgagaaggt caacgagttt atcgcggctc agggctgaaa ttcaaaggag
      421 taaaaacggc gagggcatat agcgtcctcg ccgtttttca taaggcttat ggatcataca
      481 tttaaattac cgccgcgcct tgcggccatc gccgcgctcg ttccggacgg tgcgcgcctt
      541 gccgacgtcg ggacagacca cgcgttgctt cccatccgcc ttttgctgga cggaaagatc
      601 caacgtgctg tggccacgga tatccggccc ggtccgctct cccgcgcgaa ggaaaatgcc
      661 tatgccgccg gcgtgcagga cctttcctgc gtcctctgcg acgggctgac cggcgtctcg
      721 ccggacagtg tcgatacggt cgtgatcgcc ggtatgggcg gggagaatat cgcggcgatc
      781 cttcgcgccg cgccgtgggc gtgccggaac gccctttgca tcctgcagcc gatgtcgagg
      841 ccggaggagc ttcgcgccgc gctaccgtcg ctcgggctta cgatccgcgc agagcggctt
      901 gtgcgtgacg ctggacggct ctattccgtt ctcgccgtgc gcgccggtgc tccggaggct
      961 ctttcggcag gagagctgta ttgcgggaag tattcgctgc tctcgaacga cacgctgttc
     1021 tccgatatac tcgcggaaca ggacaaacga ctcgatatcg tcgttcgcgg tctggaacgc
     1081 tccggacggg agagcgaccg tgagaggctg aacgcgctgc gccttgccgc ggcggacatt
     1141 gcggaaatga ggaggaaata ccatggcgac ggttcgtgaa atagccggtt atctggaaga
     1201 gcgtgtacca tcctcgctca aactggattt tgataatgtt gggctgctct gcggctttcc
     1261 ggaacgcgag gtgagccgtg tgctcgtcgt gctggatatc acgcagcaga ccatcgagga
     1321 ggcactgtcg cttcatgcgg agctgatcgt ctcccatcat ccggtcatct ttacaccgct
     1381 gcgccgcgtc tgcacggaca caccggacgc aaagcgcgtc attgcccttt tgcaggggaa
     1441 tctctcggcc atctgcctgc acacgaatct cgacgcgctt gagggcggtg taaatacggc
     1501 tcttgccaac gccgtcggcg gggaagagct ggagtatacg acggacatcg gctgcttctg
     1561 ccgtctgccg gaggcgatgc cgctttcaga gttcctcgcc cagacgcgca cagcgctctc
     1621 cgcgcccgat atgcggtatt acgacgccgg acgccgtgta gaatatctgg cgctctgcgg
     1681 cggcgcaggc ggggacattc tctatgaggc ggcgcagcgc ggctgcgaca cggtactgac
     1741 cggtgagatc aaacaccacc agtggattga cggcgcggag ctggggctga atctcctgga
     1801 aggaggccac ttcgccaccg aaaacgttgt catgccgtcc ctagcagaaa tgctgcggca
     1861 gggcttcccg gagatcgacg tatgtctttc acggctgcag gggccgctct cgcgcggatt
     1921 cggattctaa aacaagcata tttgcgggct tatccccata ggatgcattg aaatcatgta
     1981 cagggggtct gaccgtgtct gaatcaacga tttacgagga catcgctctc cgcacggacg
     2041 gagatatata tcttggtgtg gtgggtccgg ttcggacggg caagtccacc ttcatcaagc
     2101 gcttcatgga aacgctcgtt attccgcgca tagataatgt ctaccggaaa gagcgtgccc
     2161 gcgatgagct gccgcagagc ggctccggac gcaccattat gacagcggag ccgaaattca
     2221 ttccggaaga agcggtgacg atccgtctcg gcgaagatac cgatctttcc gtccggctga
     2281 tcgactgcgt cggctacatg gtcaagggcg cttccggcca gtttgaagac ggtgttgaac
     2341 gcatggtgac aacgccgtgg ttcgataagg agatatctct gaccgaagca gcggagcagg
     2401 gaacctataa ggtcatcgcc gagcactcga cgatcgggct ggtcgtcacc tgcgacggca
     2461 cgatctgtga gattccccgt gaggactatg tcccggcgga ggagcgcgtg atccgcgagc
     2521 tgcaggagat cggaaagccg ttcaccgttg ttctcaacag cgccgaacca aacgcggaaa
     2581 gcgcaaaagc tctgtgccga tcgatccggg agaaatacgg cacgggctgc gtctgcgtaa
     2641 actgtctgga gctgtcggaa aacgatatca gcgagatcct caaatcggcg ctgtgtgaat
     2701 ttccgctttc cgagctggac gttttcctgc cgagttgggt ggatgcgctg ccgattgagc
     2761 acccgctgcg cacatcgctc tataacggcg tgtgcgcctc ggcgcagaac attcaccgta
     2821 tc
//
LOCUS       DAWAAE010000199         2791 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_12955_length_2791_cov_3.413377, whole genome shotgun sequence.
ACCESSION   DAWAAE010000199 DAWAAE010000000
VERSION     DAWAAE010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12955_length_2791_cov_3.413377"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          551 a          800 c          882 g          558 t
ORIGIN      
        1 tgatgagctc acgctggccg cggccgatgg gcacgagtgc gtcgatcgcc ttgatgccgg
       61 tgtgcagcgg cacggagacc gactgccggt cgggaatggc gggcgcctgg aattcgatgg
      121 gccgggtctc ggtgctccag atgcggcccc ggccgtcgat gggcttgccg agcgcgtcca
      181 caacgcggcc gagcatcgcc tcgccgacgg gcacgcttgc catcttgttc agacggcgca
      241 cgcggctgta gctgtccaca tggtcgtagc ggccaaagat gatgcagccg acgcggtcgg
      301 cctgaatatc catgaccatg ccgcgctcgc cgttgacgaa ctcgaccagc tcgccgtaca
      361 tgatgtcggc gagaccgtcc atccagcaga tgccgtccga cacgctgagc acgcggccga
      421 cctggaacac gtcgatgccg agcttcatat ggtgcacggc gtcgagcata ccggcgcgga
      481 tggaatccgc gtccgtctcc gtgagaggcc ggtcgttgag agctttgccg agacggtaga
      541 gcttgttgga gatggtgtaa tcgtagatcg tatcgccgat gcggagctgc agcccgccga
      601 tcagctcttc cttttgccgc acccagtagg agatcttccc tccggcggcg gcgacgagcg
      661 cttcagcacg cttgcgcacc ttctcgacgg tctcgtcggg aagtacggcg gcggaggtga
      721 gcgtcacata cagcacgccc ttgcgggaga tgtaggaaac gtcgccgggc gtcagatgga
      781 tctgcgcgag gatctgctcg actgcctcgt tctggcgggc aagcttggaa gcctgatatt
      841 ccgccgagcg catgtgctgc tcggcgagag tgaggacctg ctcaatgatc tgcgcgcggg
      901 tcgtaaagag catttcgcgg cgctggtcgg cggcgtccgc ctcatactgc gtatcgagct
      961 ctgcaagagc ggcctttccg tccttttccg ccgcggcaag ctcggacttg agcgccgtgt
     1021 cgtccggcgc gggcatctcc ggcagcggct cgggagcgaa cggcgtttcc gcttcgtcca
     1081 gctcacgcgt gatcttctcc cggcgggagc ggaagatctt cacgatagat tttcgtccga
     1141 cgagatagat gagccccgca aggatcagaa gattgataac ggcatataca aaacgcataa
     1201 tcatttcgtc ctgttcgtag tagcattcgt atgctcaccg gcggtgagat gctctatgtc
     1261 ggcatacggc gccgcgatcg tttccgcatc cacggcggag tggcgatcgt cctgccagtg
     1321 ctgcagatgg ttcacgagat caagcgccag atcggagagc tgcggttcca gctcgcgggt
     1381 gatgcgctca gattcttcgg cgagcgcggc gcggttttct gcgagccgct gcgcggcctc
     1441 agcgcgcttt tgcgtcgttt cgcggcgcag ttcctcctgc gcttgctcaa gcgcctccgc
     1501 gccggactga cgcagctgcg cttcctgcgc agcgcgctcg tcggcaaggc ggcgcgcttc
     1561 cgcttcgtat tccgtgcgtg cgtcctcctt cgccttgcgc gcggcctcaa tcttcgcatt
     1621 gcgcgtatcc ataaaggtca gcatcggctg aaagagcagt tttttcagaa tgagcaccag
     1681 cgcgagaaag catagaaccg tccatacgat gagagatggt tcgatcgtca tagctgtgcg
     1741 cctctcagac cttggcgacg agcataaagg cgatcagcag gccgtaaatg gtaagagcct
     1801 ccatcatggc catcgagatg atgagcgagg agcggatatc gccggcggcg tcgggctggc
     1861 gggcgatgct ctccatggcg gcggcggcgg tcttgccctg tcccatagcg ggacctatgg
     1921 tgctgaaggc gatggaaatg gcggcggcaa tagcaatgat ggcggaatcg gtcatggtag
     1981 tatatctcct tacaaaaata ttctttattc ttttgctttt aagagacgcg ccgttattcg
     2041 gtggcctcct gcagataaat ggaaacgagc gtcgtgaaaa cgatggcctg cagcgcgcag
     2101 aagagcagcg agagcaccat catcacgatc ggcgcgccga tgggcaaaat ctcatagagc
     2161 tgttccgtga cggtctcttc gccgaagata ttgccgaaca gacgcagcgc cagcgacgcg
     2221 ggcttgatga actcatccag aaggagcagc ggcagcatga tcgcgatggg ggaaataaag
     2281 cgcttgagat agccgagaaa gccgtgcgca tggatgcttg agccctgcag gtagaaaaac
     2341 gtgatgatgc cgagaccaag cgttaccgaa agcgacgagg tcggcgcttt ccagtagtcc
     2401 gtcagaccgg cgccggggat gagcccggtg tagttgccga aaatgatgaa aatgaagagc
     2461 gagccgagaa acagaaagtg ttttcgggac tctttttttc cgagaatacc ggcaaagtag
     2521 ttgtcgaggt attcaacgcc cgtctcgatc acattctgga atttgccggg ccgctcgcgc
     2581 atatggcgcg tggcgagcag cgagatcacg gtgacggcgg ccagaatgac ccacatgccg
     2641 atcaccccgc cggtcacatc cagaattccg aaaatcggga tcacaacacg ggattcaccc
     2701 attgagatct tctcccttct tatccgccgc ggcggatttg gactttttat cgttgcgccg
     2761 tgccagaagc atggcaaaaa ccatggaaag a
//
LOCUS       DAWAAE010000200         2713 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_13348_length_2713_cov_3.235139, whole genome shotgun sequence.
ACCESSION   DAWAAE010000200 DAWAAE010000000
VERSION     DAWAAE010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_2713_cov_3.235139"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          607 a          798 c          793 g          515 t
ORIGIN      
        1 gccgctcttc cggttgaagc accagacgga agcgaccgtc agcagcagga gaaatccctg
       61 cgtcagatgc gccggctgga tgcgatcgct cgtgaagaag agaaaggcat agtttgcggc
      121 gaagagtgcg gcccggagga cattatttcg ctttttcatt gtcggtcgtc tgtgcggcgt
      181 ggtacatgcc ggaaaggatc gtcatctgca gctcaaaatt ctgccggtgc atcttgccct
      241 gcgtatcgag gatcagaccg catatgaagc tctgcagaga gaacagcgcc ataaagcatg
      301 ccgccacgaa cgtcgggagc cgcggtacga gcccggtgtg caggtactcc gtcagcaccg
      361 ggtacagaaa gagcagcgcc gcgaccatca gtatcccggc gatgatgctg aaaaacggca
      421 ggggccggta gtccttataa agccgcgcaa tcgtccgcaa aaccttgaag ccgtccgaaa
      481 aagtgttcag cttggactcg ctccccgccg ggcggtcccg gtatccgacc gggatcgacg
      541 tgagcgcaaa gtttttgtcc agcgcgtgga tcgtcatttc cgtttcgatc tcaaagccct
      601 tggaaaggat cgggaagctc ttgacgaaca gaggactgaa cgcgcggtat ccggtcatga
      661 tatccgtcac gtgaccgccg aaaaagcggt tgaccagctt tcgcacaagg acgttcccgc
      721 tgttgtggaa cgggcgcttg ttctccgtga aatatgtgga ggaaaggcgg tcgccgatca
      781 ccatgtcgta gccgtcgcgc agtacgccat ccgccatggc acgtgcatcc tccgccgggt
      841 aagtgtcgtc tccgtcgatc atcagatagc actccgcatc gatctcgcgg aacatcgtcc
      901 ggatcacgtt gcccttaccc tgccgccgct cgtagcgcac gacggcgccg gcggcgcggg
      961 cgatctcgtc cgttccgtcg gtggagttgt tgtcgtaaac atagatcgcg gcttccggca
     1021 gcgccgcgcg gtagtccttt accaccttgg cgatggtggc gctctcattg tagcacggga
     1081 tcagaacggc gattttatcc atgttcgtcc tccttttccg gctctgcatc cgaaagttct
     1141 tttgcgggtt cggttggctg tgcgccgtcc caattctgct tcaccgcgac ggtgataaag
     1201 agcagcgcca tcacgctcac ctgaacaaaa taaacgtacc ggaagctctg gtgatagagc
     1261 accagagcag aggccgcgat attccccagc atgggaaccg cagcccacca gagccgcccc
     1321 atacgatttt tgttccagaa gagaagcagc gccagaaatg cgatcaggta agcgccggta
     1381 cgccagagca gcatatccgc cgcgctgttg atcggagtat ttgccgcgga atagtacgct
     1441 ttcgctggca cagcggtttt tatatacgaa ccgtcgtcca gccggtccag acggctcgta
     1501 tccagcggga gcgtgttttc ggaaaatgtg taaacaaagt tgaaggattt tgcgttgaag
     1561 ctgtcgggcg gctgaacgac gtcccagaga atgtccgtgc cgtccagacg atccttgatg
     1621 actacgtccg ggtatttccg cagtgcctcc agatagaccg taaaggcgtc gcgtgcggtg
     1681 atatcagaga tcttatacgg ctcggagcct gccggacggc cccagtaata gggatcatgt
     1741 cccacaaagc gagagtaata ttcgccccag tcctccagcg gcagcgcctt ttccaagatc
     1801 tgctcgctct cctccgagag cgggagctcc ttgttgatgc aggagcctgc ggcgcacatc
     1861 atggtcgtat acggggacat gccgttcggc acaacgccaa gcgccgtgaa taccggcccc
     1921 ttatacaccg cgagaagcac cacagcggtc agcgccgaga cggcgggtct ccattttaaa
     1981 gcggcatggt ttttcagcgt cagaacgatg cacagcaccg caacggccag acccggcaca
     2041 acgccgttgt gccgcagcgt gcagatgaga aaaatatctg cagtcagaca cacataaaac
     2101 ccgaacttcc tgctctgcgg ctttttcatc gccagcagcg caagcagata aagcccccat
     2161 aaaagcgcga gcgtgaacgg aaaatccttg agtacgttcg acgccgagag cgcctgattc
     2221 ggcagaagct cgatcaccgc gccgagaaat gtcagccgcc ggaggggaat gccgctgtcg
     2281 tacccgatca tcaaaatggc tgtcagcagc catgtgaaaa ggagcatctg cacggcagtg
     2341 atcgcaccgg cctgcggaat gacggtcaaa atgagctttt ccagcagcgt gtgcatcacc
     2401 ggatgccagt cgttgatgcc gtaaagcccc gtcgcctgcg aaagctggtc gatggcgtcc
     2461 gccgggaagc cgcccggcca caggatccac aaaacgccca gctgacacac ggccagtacg
     2521 gcaaagagcg tccaccacgc gcggcggcgg cttcctggtt tcggcagcgt gcggggacgg
     2581 gcggagagcc attccagcgc gaacagcatc agccacacga ccgggatgaa ccagagaata
     2641 ccgcttagca catagaaaag ccccggcagc gaaaagtgca tcctcgtgtt gccgtccaga
     2701 aagaagcgct gcg
//
LOCUS       DAWAAE010000201         2709 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_13378_length_2709_cov_3.000754, whole genome shotgun sequence.
ACCESSION   DAWAAE010000201 DAWAAE010000000
VERSION     DAWAAE010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13378_length_2709_cov_3.000754"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          735 a          579 c          646 g          749 t
ORIGIN      
        1 ccttcaccgg cgacatcgcc gcgtcgatct gcctcggcgt tctgaccgac gtgcccatca
       61 aggtagtgac cggcgatgcg aaaaaggtcc acccggtgct ctatgtactt tgcatccccc
      121 tcgttcttta ttttatagtg taacgggttt tgctgaaaaa ggctctcaga tctgtgatct
      181 gagagccttt ttctttatat taatattata tacagcctgt cgagaaaccc ggctgtgcgc
      241 taagcggtag ccgggtttct ggcgtattga gagtggaaaa acaagatgtg gaaatagtca
      301 ttccatttcc atatcgccag ctttttcaag ttcatggcag cgaacttagg ctgtgcggtt
      361 ttattcttta ttctgcttca aggaggaaag gtctttcaaa accgccagtg ctatcggttt
      421 tacatctggt gtgcaggact gaaacagttt cagcaccgtt tcactaccgt aggttttttg
      481 agcgtaccgg cagccggata ctacgtaacc gagatcggca tcaaccgccg cgcatacagc
      541 gtcgagcgta tctagcgtcg gcgtaacttt gccgttttca atttttgaga gatacactgt
      601 cgtaatatct gcagcttccg caaccttttc ttgcgtcaac tttgctgtac ggcggctttc
      661 tttcaaacgc tttccgatca ttgcataatc aattgccatg aaattaccca tattagtata
      721 gcaatatgat acggatagta taataaatta ttagattaac ttgatttatt aatttaacaa
      781 tgatagtata gagatacaat tctatataaa aaggagagat atgattggat acggagtata
      841 aagaaatgac tactgcagca gaaactgatg caactgtttc tgcgccatcc ccagcttctc
      901 cggtgcctgt tggagaagct gaatctccga agaaaaaagt gccgcgttgg atatggctgg
      961 ctctgagtat ttttcttctt gctgcggcag cagtcatttt cttcgtcggc atagaatcat
     1021 caggagattc cgacgattat ctcgctgagc tgaactacgc gaatgggggg cttgttgcct
     1081 atgacagtga acatgtatac tatcttgctc cagctgagac aggatcgaaa aaaagcgagt
     1141 atatactcgt atatgaagca gatccgtatg gaaatgatcc tcatgtagtg tgcaaggaaa
     1201 aagcactcga aacgatccgt tccgcagaat ccgaactgta ctgttttgga aagcaaggct
     1261 cggacaacac gccgttcctt gctaggatca ataaagcaac tggcgaattg accgtgctgc
     1321 gcagttttcc ggcgggaacg gatattacat attttaccgt tcgcgattcc gaactgttct
     1381 actgtgcaaa tagcacactt tacaaaggtg ctttggagga agccgagccg accgcagacg
     1441 ttgccttgtt taacggctgc gctgccgtgc atttttccgg tgatgcagtt tactattctt
     1501 ctgaaaatga ggtatttgta tacaaactcc gttcggggaa ggtccggtcg ctttgctccg
     1561 ctgccgccac acagatatgc cgcaagggaa accaactttt ttttatgaat caggaagggc
     1621 tattctccgt tagaagtaaa ggcggagaag cgacaaaact cgttgcgcat gatagttccg
     1681 aaacctcttt cttttccttt ttttcacttt acaaggataa tattctgtat ggatatggct
     1741 ttgatgcaga tgaggttggt gaacttatcg acgctcatac gggaacttct tattcggacc
     1801 aaatatatgc gcctattttg aagataatcc tgttgtttaa cggaaatgtg gagtatatcg
     1861 gagaaaatgg cagcgagccc accggaacgg aaagaccaat ttgcgctatt ccggattatg
     1921 ccgtcttttc tccctatgga atcgcttcag gtttctatat taccccgtat caatcgtact
     1981 attccagaaa ccttgtccta tatacattag ttgaaattat ggagtttaaa taatcaaaaa
     2041 aggatgtacc acgtgtatga gaaaaacaaa aaaagctgtt gccgccattt tgacgctgtt
     2101 gctgatagta tcaatgaccg cctgcgccct gcacggaaaa aatctgacta tggatgcgct
     2161 tgtgaatgca agcacaagag acgaagtgat agaactgctc ggcgattctt tttctgcagc
     2221 gggagatagc gactggtatg aatccgttga atatatggga gaaccctatt ccatgcttgt
     2281 tagctactgc gacaacaccc ttgacaacat tgctatgcat tgtgactatg aaggaatgcg
     2341 cggcatggac ttggtcctgg aaagactcaa ttatacggtg acagcgagag agcggcagct
     2401 cgcaaaaaat gctctcgaag aagttgcgtg tgagcttacg gaaaaatatg gtgaaccgga
     2461 ggtttactat tcaccggtcg agacaacgac ccgcagctgg tacgtcggtg actgtgagat
     2521 tgagcttgaa gatcacaccg gaaacgatca gttcaaggcc ttgggagcct atgagatctt
     2581 gatttggtat tgaccgggaa aaccagaact atatgacata aattacagca gaaaggcaca
     2641 gtgtgagtat ggagacaaag aattgtccag aatgtggtca tgagatgaag gccgaggaaa
     2701 ctctatgcc
//
LOCUS       DAWAAE010000202         2707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_13390_length_2707_cov_3.186275, whole genome shotgun sequence.
ACCESSION   DAWAAE010000202 DAWAAE010000000
VERSION     DAWAAE010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_2707_cov_3.186275"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          464 a          877 c          826 g          540 t
ORIGIN      
        1 tccgctggat cgtggacgac tgcgccaaat tcgtcgagcg ggagatccgc cgcgggcaca
       61 agtatgacgc catcatcatg gatccgccgt cctatggccg cgggccgggc ggcgaggtat
      121 ggaagctgga ggacaatctc tgggacttcg tttcgctctg ctccggcgtc ctctcggacg
      181 acgcgctttt cgtgctcatc aattcctaca cgacgggcct tgccccctcg gtgctctcgt
      241 atatcaccga cagcatcttt accgcccggc gcggcggttc ctcgcgcgcg gaggagctgg
      301 ggcttcccgt cacgcagagc ggactcgtgc tcccctgcgg ggcgagctgc cgctgggagg
      361 cttaaatggc aaattccgtc atcactctca ataacgtatc cttccgctat cccgacgccg
      421 ggcgcgacgc gctctccggc gtcagcctct cgatcgaggc ggggtcgttc atcgccgtgc
      481 tcggacacaa cggttccggc aagtccacgc ttgcaaagct cctgaacgcc attcttgtgc
      541 cgacggaggg taccgtgacc gtcaagggca tggacacgcg cagcgaggaa aaccttctta
      601 acgtgcgccg gacggtcggc atggtgttcc agaaccccga caaccagatc gttgcgagcg
      661 tcgtggagga cgatgtggcg tttgcgcccg aaaaccttgg cgtttccccg gcggagatcc
      721 gccgccgcgt ggacgaggcc ctgaagcgcg tcggtatgta cgaatttcgc aataacgcgc
      781 cgcaccttct ctccggaggg cagaagcagc gcgtcgccat cgcgggcgtg ctcgccatgc
      841 agccggagat ccttgttatg gacgagccca ccgccatgct cgacccctcc ggacggcagg
      901 aggtgctgcg cacgatcgcc gatcttcgcg ccgctacggg cgtcacagtc gttctcatca
      961 cgcaccacat ggatgagtgc atcgacgccg accgcctgct cgtcatgtcc gacggcaagc
     1021 tcgttctcga cggcgcgccg aaggacgtct tttcgcgcgt tgaacagatg cgttcgatcg
     1081 gtctggacgt tcccgccacg acggaaacgc tctatgagct gcgcgccgcc ggggcggatg
     1141 tgccgctcgg cgcgctctcc gacgcggaag ccgccgatgc cattgtccgc tggctcggaa
     1201 attaaatcca tcagtaaagg tttttcgtta tgcccgatat ccgtctggag tctctccggt
     1261 gcgtatacag cgccgggact ccgtttgaaa aggtcgctct cgacaatatt gatctctcca
     1321 tccccgccgg tcagtacgcg gcgctgctcg gtcataccgg cagcggcaag agcacgctca
     1381 tccagcatct caacgggctt ttgaagcccg ccgcggggcg cgtgctgctc ggagaagagg
     1441 acatccaccg ctccaaggag gcgctgcgcg ccgtccggtt ccgtgtcggt ctggtgttcc
     1501 agtacccgga ataccagctc tttgaagaga cggtttaccg cgacattgcg ttcggtccga
     1561 aaaacatggg attgaacgag caggaggtgg acgagcacgt ccgcttcggc gcaaagtttg
     1621 ccggagtcga tgaatcgctc ttcgaggcgt ccccgctcga tctttccggc ggacagaagc
     1681 gccgcatcgc gattgcgggc gtgatggcca tgaagccgga ggtgctcgtg ctcgatgagc
     1741 cgatggcggg tctcgacccc gccgggtgcc gcgagatcct tgcaaacatc gccgaatacc
     1801 atcgctccac ggggagcacc gttgtgctcg tcacgcacga tatggacgtc gccgcggaaa
     1861 acgccgagcg gctcatcgtc atgcggcgcg gcaagatcgc gctcgacggc gcgccggagg
     1921 agatcttcac ccacgccgcg gagctttgtg aaatgggtct cggcattccg ggcgcagccg
     1981 ctctcgcaga ggagctgcgt gcgcgcggcg ttgctgttcc gcagggtatc tataccccgc
     2041 acgatctcgc cgcggccctg ctcgcaagga aggagggctg cgcatgctga aagacattac
     2101 gctcggccag tacttccccg gcaataccat cgtgcaccgg ctcgacccgc gcacgaagct
     2161 catcgccgtg gtgctctata ttgccgcgct cttcaccgcc aacaactgga gcgctactgc
     2221 gctcgtactg ctcacgctcg ttttctgcgt ggcgctctcg cgtatccgcc cgaaggcgct
     2281 cttcaagggg ctcaagccgc tcatcgtgat cgtgatctta acggcggtgc tcaatctctt
     2341 ttactcagac ggcaatgtga tctgctcgtt ctgggtgctg cgcatcaccg ataacggcat
     2401 ccgccgcgcg gtactgatgg tgctgcgcat cattctgctc gtgtgcggaa cgtttcttct
     2461 gacgtatacg acctctcccc tgcagctcac cgacgggctg gagcagcttt tgagtccgct
     2521 caaaaagctg cgcttcccgg tgcatgagct tgccatgatg atgaccatcg cgctgcgctt
     2581 tatcccgacg ctcatcgaag agaccgacaa gatcatttcc gcgcagaagg cgcgcggcgc
     2641 ggattttgaa agcggcagtc ttgtccgccg cgcgaaggcg ctgctgccga tccttgtgcc
     2701 gctgttc
//
LOCUS       DAWAAE010000203         2643 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_13729_length_2643_cov_2.080371, whole genome shotgun sequence.
ACCESSION   DAWAAE010000203 DAWAAE010000000
VERSION     DAWAAE010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13729_length_2643_cov_2.080371"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          862 c          830 g          513 t
ORIGIN      
        1 ccgcgagaag cacgcttgcg cagaggatga aagcagcata cataatgccg cccatgcatc
       61 cgatgcgccc gtcgcgccgg gagcggattg gcatatacgc acgctccgcc tcgcgcgggt
      121 ccagctcggc ctcgaccggc tgcggcgcgg ctttcttctt tttcggttcc ggctccggct
      181 tcttttccgg acgcgccgtc cggacggagc gcaccggctt tttcttcgcc ggcttcgcgc
      241 tcttcgccgc cggggacttc ggcgtctccg gcgctttcgg tgcggcggtc tccggcgctt
      301 ccggcgcggc cgggacaccg gcagccttta ccggctcctc cgccggaggc ggcgtgggcg
      361 ccatgtgtcg cccgatgtgg ttttcctctt cgggccggat cttttcttcg tcagacataa
      421 atgctctcct tggagttgta accgcccgcg gcgcacgggc atagaatacc gcagacgggg
      481 cggaaagccc cggcggaact gcgaaacgat gttaccgacc ggccggagcg ccgtgcgcgg
      541 ccggggcgca aatatggcac ggggaaaggg tattctccat gttctgtgga aataataacg
      601 gctgtctctg gatcatcatc attctcatcc tgctctgcgg ctgcgggtgc ggcggctgcg
      661 gcaacggctg cggctgcgga aacaacaacg gctgcgggtg cggctgctga cttattttgc
      721 gagacccctt cgggggtctc tttttttatt tccgctcgcg cgccgcgaga agcagtattt
      781 tgggcagacg gatcatgcgc ttgaggcgct tcggctcgcg cagcagccgg tagagccatt
      841 ccagtcccag ccgctgccag ctctccggcg cgcggggaat gtctccggcg aatacgtcga
      901 gcagaccgcc gacgccgatc atgacggcat ttattttctg ccggtttcgc gccatccaca
      961 gctcctgccg cggcgcgccg agaccaagca gcagcagatc gggttttttc tgttccagcg
     1021 ctgcgaaaag cgccgtttca tcggaaatat acccattttc cgctccagcg atacgaaggc
     1081 cgggataagc gcttttcagc ttctcccccg ctctttgcgc gacgccgggg cgcgcgccgt
     1141 aaaggaacac gctgccggag cgctgcgcga gccgggaaag aagccgcggc acaaggtctg
     1201 cgcccgtaac gcgctcggga aggggcgttc ccagaatgcg cgcagcgcag agatcgccca
     1261 cgccgtccgc gagcacgagc gccgcgccgt tgaccgcggc ggcatacgcc gcgtcctccc
     1321 ggcagtgcag cacgatctcg gggttggccg ttacgatata tccgccggta ccggagcgca
     1381 gcagcgcgtc ggcgcgctct tcggcttcat cgagcgtcag cgcgtcgaac gcgacgccga
     1441 gaatgtctgt cttcatacct cggaaaatac cttgccgatc ttttcgcgga tgagaaggtc
     1501 ggccgattcg tcatagctcg tagcgcccat attcacgagc acgagcttgc ggccttcgta
     1561 atagcgcagc agtcccgccg cgggatacac cgcgagcgac gtgccgccta cgattaggac
     1621 gtccgccttc cggataaagt tcacggcgtc ggtgatgtcg cgctcggcga gcggctcttc
     1681 atagagcacg acctccggac ggagcacgcc gccgcacgag cagcgcggca cgccctcgcc
     1741 ggtgttgacg gccttctcgg gacagggctt gccgcatctg gcgcagcggc agcggtggat
     1801 cgaaccgtgc agctcaatga cgcgcttgct tcccgcggcc tgatgcagcc cgtcgatgtt
     1861 ctgcgtgatg acggcgcgca gttttccttc ccgctccaac tcggcaagcc gtgctgcgga
     1921 aatccgggat gccggattcc gtggacacgc ccgcgccgcc gaaaaagacg atgttgccgc
     1981 tcccggcgat ccactcgcgg agcaccgcga tattgtcctg catggaaaca ctcccccctt
     2041 ttactaaggg tattatacca actttttccg atttgcgcaa cagaaaacgg gagacggcga
     2101 tcccgtctcc cattttatca taatatgaaa ggcctcgcag agtttgcgct gcggcgagcc
     2161 gatatttcct caaacgggag tcccgctgtg cggaactccc gtttgaatgt ctttaggtat
     2221 tcggccccat ggtgtagttg ggcgcttcgc gggtgatgta gatatcgtgc gggtggctct
     2281 cgcgcagacc cgcggcggtg atgcgcacga actgcgcggt ggtgcgcagc gtttcaatgt
     2341 cgggcgcgcc gcagtagccc atgccggagc ggagaccgcc gaccatctga tacaccgtgt
     2401 cggacaccgg tccgcggtac ggtacgcggc cctcgacgcc ctcggggacg agcttggagt
     2461 ttttctcctg gaagtagcgg tccttcgagc ccttcgccat cgcagcgagc gaacccatgc
     2521 cgcggtagac cttgaactga cggccctgga acacctcggt atcgcccggg gcttcctcac
     2581 acccggcgag catggagccg agcatcacga cgctgccgcc cgcggcaagc gccttgacga
     2641 tat
//
LOCUS       DAWAAE010000204         2588 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_14058_length_2588_cov_3.018555, whole genome shotgun sequence.
ACCESSION   DAWAAE010000204 DAWAAE010000000
VERSION     DAWAAE010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14058_length_2588_cov_3.018555"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          421 a          780 c          841 g          546 t
ORIGIN      
        1 cgatatagca gtaattttcc ttgcggggct ttgcctccgg gtcgggggcg gcggggtggc
       61 cgaccgccac ggagccgacg ccgcgccagt cgccctccac gccccagcga cggagcattt
      121 cgcggccctc gttcgtttca aacatgaact gcggccggtt gatccagcag gacgcgagcc
      181 cggcggcgta tgcgccgttg cacaggttcg tgagcactgc cgcgccgtcc agatcgccga
      241 gcgggaaggt gcgcggcacg agcacgagca gaatggtggg cgcgccgtag taggggttgg
      301 cgttcggggt gaacttcgcg ttgagcgcgt tgaccgcggc gacctcgtcg ggattctgca
      361 ccgcgatgat gatcgggctc tggccgtttt tggcggtcgg cgcgttcata ccggcggcga
      421 gcaccttgtg cagctcctcg tcggtgatct gctcgggcgc gaacgctcgg ttcgagcggc
      481 gggaagtaag tccgcgcagc atttcctcat tcatggtttt gccctccttg tattttttta
      541 tatttttgaa atcagccctg tacggccttt ttcagcgctt cggtgcggtc ggtcttttcc
      601 caggcgacgc cgagatcctc gcgtcccatg tggccgtagg cggcgagatg gcggtagatc
      661 ggcttgcgca ggtcaagatc gcggatgatg gcggtcgggc gcaggtcaaa gaccttcttc
      721 acggcctttt cgagcgtctc atcggacacc gtgccggtgc cgaacgtcgt aacgttcacg
      781 ctcacgggct cggccacgcc gatggcgtaa gcgagctgca cctggcagcg atgggcaaga
      841 ccggcggcga cgatgttctt cgccacatag cgcgcggcgt acgcggcgct gcggtccacc
      901 ttcgtcgggt ccttgccgga gaaggcgccg ccgccgtggg gagcgctgcc gccgtaggta
      961 tcaacgatga tcttacggcc ggtgagaccg ctgtcaccct gcgggccgcc gatgacaaag
     1021 cggccggtgg ggttgatgaa gaagcggata tcgcccttat tgagctcggc ggggatcacg
     1081 ggcagcgcga cctcgcggat aagatcctcg cggatctgct caagagagac ctccggcgcg
     1141 tgctgggagg agatgacgac cgtgtcgatg cgcacggggc ggttgtcctt gtcgtactcc
     1201 acggtgacct gggtcttgcc gtccggacgg agatagtgga gcgtgccgtt cttgcgcacc
     1261 tcggtcagac ggcgcgccat tttatgcgcg agagagatgg gcagcggcat gagctcgctc
     1321 gtttcgtcgc aggcgtagcc gaacatcatg ccctggtcgc cggcgccgtt ggtgagatcg
     1381 tcgccctcgc cgccctgctt gcgctcgagc gattcgttga cgcccattgc gatatcggcg
     1441 gactgctcgt ggatggacgt gagcaccgcg caggtctcgc agtcaaagcc gaacttcgcg
     1501 cggtcgtagc cgatgtcgcg cacgacgtcg cgcacgatct tcgggatatc gacgtagcag
     1561 ctcgtggtga tctcgcccat gacgagaaca aggccggtcg tggcagtgca ctcacacgca
     1621 acgtggccgc cggggtcctt ttcgagaatg gcgtcgagca ccgcgtcgga gatctgatcg
     1681 cagatcttat cgggatggcc ttcggtgacg ctttcggatg tgaagagata gtttgccatg
     1741 gtttcctcct gatgtaaaaa aatatcgccg cgcctctggc cgggatgatc ccgtgcttac
     1801 ggcggagcga taaaaaatct tatctttcga cttccgccga cggatttggc accttatgct
     1861 cacgcacagg ttgtcgggct tcacagggcc gttccctcca ccactcttga taagccgtat
     1921 ttggtttggc gattcaagaa ttctgccggt ttacccggcg tctattgttc taccacgttt
     1981 ttcgcgcctt gtcaacgccg cggggcgaac aaaatccgtg gttttgtacg ccccgcggcg
     2041 aaattcgcgt tatgtatagg atttatcgat ctggatgatg caccggcagt ccgggtggcg
     2101 ctcctccacg gcggcgcgga cggctttgcg cacgtcctcg tccgagagct tatacccgaa
     2161 cggcacgagc acgtcaaaga cgagattcgt ccggtgcttt cccggcacca tgcggaaatc
     2221 gtgcaggctg agcacgggat cgatgctccg cagaagctgc accgtttcct cgcgcatctc
     2281 cgccagccgc tcgtttttcg tgtccacggg gtccatgtgg atgacagaga agcagccgag
     2341 cttgtggtcc agctcgctca ttgcgcggtc gatcgcctcg tgcagctcat aaatgttccc
     2401 ctcgccgtcc acctccgcat ggagggaaac gtggcagcgg ccggggccgt agtcatggat
     2461 aacgagatcg tggatggcga gaacttccgg gtacgacagc acgatctgcc gcaccttttc
     2521 cgcgagctcc gcgctcggcc gctggccgag gagctgcccg agcgtttcct ttccgccctc
     2581 gtacccgg
//
LOCUS       DAWAAE010000205         2468 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_14807_length_2468_cov_2.576046, whole genome shotgun sequence.
ACCESSION   DAWAAE010000205 DAWAAE010000000
VERSION     DAWAAE010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14807_length_2468_cov_2.576046"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2047..2056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          454 a          776 c          765 g          463 t
ORIGIN      
        1 gtacagtata tattatatcg aacggaatgt caaatataaa ttgcaataag ctcatatatt
       61 tttctttagg ctattataaa cggtgcttgc aatgcgccaa gagcagccat aagtcagcct
      121 tgccatttaa tgtagaaaac cattacatcg tcaataaaac agaaaaagca ctcggcaaaa
      181 ggcttcacgg caagccgggc tttgcgcaag aaaacctttt attttatata gtccggctgc
      241 cgttgcagca aacgcgccgc cgcagctctt gacgctgcgg cggcgcttga gtacaataga
      301 ttggttaaag ggaagggtag agctcctttc gcgccgcggc gagcgcttcg caaaaataag
      361 cgcattcctc cgcggtcgtg tagcgcgaga acgaaacgcg cagcgccccg tcgatgacct
      421 tgtccggcgc gccgatggcc tcgagcacat ggctgcgctt gccgcgcttg cacgcgctgc
      481 tcttggaaac gtaaatgccc ttcgcttcga gatagttcat caagacctcg ctgcggtagc
      541 cgggcaggga gagcgagaga atgtgcgcgc tgccgccgcc gaggaataca atatcgggta
      601 cggtgtcggt gagcgtctgc acgcactgag cgcgcagcgc ctccatgcgt ttgttcgctt
      661 ccggcatttc ggacttggca atgcgcacgg cctcaccgaa ggcggcgatc tgcggcatcg
      721 gctcggtgcc ggagcgcagt ccgtcctcct gcccgccgcc gagaatgtgc gccgggagct
      781 tcagcccctg ccggatatac agcgcgccga tgcccttggg cgcgtggacc ttgtggccgc
      841 tcacggatat catgtccgcg ccgagcgttt tggcggagaa cggctccttc ataaagccct
      901 gcaccgcgtc ggtgtggaga agcgccctgg actttgccgc gcgcagcgcg cgggccacgc
      961 ccgcgatgtc cgtcacggcg ccggtctcat tgttgaccat catgagcgtt acgagcgccg
     1021 tatcctcccg gagcgcggcg cgcaccgcat cggcggatat cgcgccggtg ctgtcggggg
     1081 agagaagtgt tatctcatag ccctcgcgcg cgagcttttc gagcggcttt cgcacggcgt
     1141 cgtgctctac ggcggaggag atgatgtgcc gcccgacgcg ccgctggaga tatgcgcctt
     1201 ccagaatggc ccagttgtcg ctctccgtgc cgccggaggt gaagtacatc tctttgggag
     1261 cgcacccgag cgcggcggcg agctttttgc gggaatcgtc cagcacagcg cgcgcctcgc
     1321 gccccagctt gtgcgtcgag ccggggttgc cgtacacttc cgtcatcact ttatacgccg
     1381 cgtccgccgc ctccgggcat acgcgggtcg tggcggcgtt atcgagataa atttccatat
     1441 ccacacatcc tttgaggagc gtctttctat gaagtacatt atagccactc tcggctgcaa
     1501 ggtcaaccag tacgagtccg aggcgatgga gcagcttctc cagagccacg gccacacgcc
     1561 gtgcgccgaa ggggagagcg cggacgccgt gatcgtcaat acctgcgccg tcacagccga
     1621 aggcgcgcgc aaggcacgcc agaccgtgcg ccgtctccgg cgcgaaaacc ccgatgcgct
     1681 cacggcgctc tgcggctgct ggagtcaggt ggacgccgcc gaagccgcgt cgctcggcgc
     1741 ggacgttctc ttcggcaccg gtgacaggca gggctttgtc cgcgccgtgg agaccgccgc
     1801 ggcggagcgc ggggcaggag ggagcgtaac gaagctcgac gatcccttcc ggcgcaccgg
     1861 ctttgaagag ctgcccgccg gagcctatgc cgggcacgcg cgcgcgtatc ttaaaattca
     1921 ggacgggtgc gacaatttct gcacctactg catcattccg ttcgcgcgcg ggcgcgtcct
     1981 ctccctgccg gaggaccggt gcgcggcgga ggccgcgtcg ctcgccgcgc agggctataa
     2041 ggagatnnnn nnnnnnggcg ctcccggcgc gcgcatccat ctcggctcgc tcgagccgac
     2101 ggtcgtgacg gattcattcg ttgagcgcct gaaaaagctc aacgtttgcc cgcacttcca
     2161 tctctccatg cagtccgggt gcgacgaaac gctcgcgcgc atgcgccgga agtatacgac
     2221 cgcggagttt ttcgccgtca ccgagcggct gcgcgcggcc tttcccggct gcgcactcac
     2281 gaccgatctt atcaccggct ttcccggcga gacggaggaa gagttttcca agacgctcgc
     2341 cttcatcgaa aaatgcgcct tcgcgcaaat gcacgtgttc ccgtattcga tccgccccgg
     2401 cacaaaggcg gcgggtatgt ccggccagat cgaaaagagc gaaaaggccg cgcgcgccgc
     2461 gagagccg
//
LOCUS       DAWAAE010000206         2426 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_15077_length_2426_cov_2.152256, whole genome shotgun sequence.
ACCESSION   DAWAAE010000206 DAWAAE010000000
VERSION     DAWAAE010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15077_length_2426_cov_2.152256"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          708 c          682 g          520 t
ORIGIN      
        1 aagcccttcc gctcggcgta catttcgtac atccggaaaa gatcggcggc aaaaagcgcg
       61 ctctcctctc cgccgacgcc gccgcggatc tccacgatga cgtttttccc gtcgtccggg
      121 tccttcggca gcaggaggat cttcagctcc tccgccgtgc gttccatgcc ggagcgcgct
      181 tcgctgagcg tttcgcgcgc aaggtctttc atttcggggt cggagagaag ctcttccgcg
      241 ctctcggctt cctgccggaa ggtctcatag cgcgcatagg cctccatcag aggggcaagc
      301 tctgccttct cgcggctgag cgccgcggcc ttctgcgcgt ccgcccagac ttcactctgc
      361 gagagcatgt attcgatctc cgcgtatttc tgcgagagac ctttcagctt ttcgagcatc
      421 cgcgcgcccc ctctcttcca taataaataa ttttaccaca cgccgccgcg aatgtaaacg
      481 cacaaaaaat ccgtccccgg aaaaccgggg acggatatgt gaagagcata cttacgccat
      541 ttcactacgg tattcaagtc cgcaaatagt gcagcgttca ataatgacaa ctttgttggg
      601 gtactgaatc ctttccgttt ccatgttgtg ctcgcccatt tccgcatagc cgcaggcgca
      661 ctcacgtcca tggtgcatgc cgtcgtccag cgggatccat ggggtaaaac tgtggacgtg
      721 ctcaggctct tcgcccggct tgctccggcc gcacacggtg cagctgccat ccttatagga
      781 atggttgccc tggcagaggt caaactcaat gccgaagcag tccttgcagg tgccgtaacg
      841 cagaccgcgc cagcggcagt tctgattgaa aatgcggtgc cagccatagg tgtgctcgct
      901 gtcggcgtcc ttttcggggg cgtggttttt tacgagatcg ataagctcct ttgatggctc
      961 atagccgtcg gcaacgaaaa tgcgcgggcc aacagcggta attttgatga taagcggcat
     1021 ttccgcgacg agctcgcgct cggtcttgcc gtcctttgtc gtttccttgt accaggaaac
     1081 ggaggtttcg gtctcttccg tgatggcgtc gcggtcaaca aagaagagcg cgagcccctc
     1141 ggcggtgaga agcgtgtcgt cggccggctc gagcgcgatg gcaacggttt cgtccgctgc
     1201 cgctgcgccc atggtgcaga gcgagagcgc aagcagaagc gcgagaagaa gggcggtgat
     1261 ttttttcata gaggcctcct tttgatcgaa agattctttc agatggcgca ccggtgtggt
     1321 gcgtgtgcca attataccat gagcggcgga ggaatgcaat ccgtttcttc gcgccgggcg
     1381 gcgatttggc aaagttcacg gtttgttccc aatctcgccc ttgacaagaa acgccggaaa
     1441 gcttataatg cctacgttaa aagccgaaag tttgaacttc caaagcgttt cggcgcctgc
     1501 ggagggaggg atatagaatg tctgaaatcc atgtcaagga aaacgagtct ctggatgccg
     1561 ctctgaagcg cttcaagcgc tcctgcgcga aggccggagt tctggctgac ctgcgtaaga
     1621 aggagtacta ccagagcccc agcgtcaagc gccgcaagaa atccgaggcc gcccgcaaga
     1681 acaagcgcta ttattgattt tgatcgcaca gatccccgct cgtccgagcg gggattttgt
     1741 ttttgccacc cccccctttt tttggagttg acagcccttt cgcgatgcgc taagatagga
     1801 aaaatacccg aaagccatgt gcagaatacg atcgaaaaaa caggaggata tggtcttgaa
     1861 cagtgcttat gccaaccgtt tcgtcggcga agggctcacc tttgacgacg tgctgctcgt
     1921 cccggcggag agctccgtcg ttccgcgcga tgtgaagctc gaaacgcatc tcacgaagaa
     1981 gatccgtctc ggcatcccca tcatgagcgc ggccatggac accgtgaccg aatcacgcat
     2041 ggcgatcgcg atcgcgcgcg agggcggcat cggcgtcatt cataaaaata tgacgatcga
     2101 gcagcaggcc gagcaggtcg atctcgtcaa gcgcagcgag aacggcgtca tcaccaaccc
     2161 gtttttcctt tccgcggagc acacgctgcg cgatgcggac aatctctgcg ccaaataccg
     2221 catttccggc gttcccatcg tggacgaatc cggcaagctc gtcggcatca ttaccaaccg
     2281 cgatatgaag tttgagacca accccgaccg gaagatccgc gagctcatga cgagcgaaaa
     2341 tctcgtcgtc ggccgcgagg ggaccacgct cgaggaagcc aaggagacgc tgcgtcagca
     2401 caagatcgaa aagctgccca tcgtcg
//
LOCUS       DAWAAE010000207         2168 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_17104_length_2168_cov_3.231898, whole genome shotgun sequence.
ACCESSION   DAWAAE010000207 DAWAAE010000000
VERSION     DAWAAE010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17104_length_2168_cov_3.231898"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          577 c          675 g          428 t
ORIGIN      
        1 tgatcttggt catgcccttg ccccacatat tgaagccgat gacgatcgcg atggcgacga
       61 cggcgacgag ccagttggca gagcagttgg tgatcgcgga ggaggagagc gtcaggccga
      121 tggcgatgat gatggggccg gtgacgatcg gggggaagaa acgcatgact ttcttcacgc
      181 cgaaggcctt gaagagcgtg gagaggacga cgtacatgag acccgccgcc gcgacgccga
      241 cgcaggcgta aacgagaagc tcgggttcct tgttgggggc gatggcctga tagccgccga
      301 tgaaggcgaa cgaggagccg aggaacgcgg ggattttgcc cttggtgagc agatggaaga
      361 gcagcgtgcc gagaccggcg aagagcagcg tggtggcaac gtccagaccg gtgagcgccg
      421 ggacaaggac ggtcgcgccg aacatggcga acatgtgctg cagtccgagc accagcatgc
      481 gcggcgtgcc aagggcgcgc gcgtcgtaaa tgggttcgga tccgatttgt ttctttgtca
      541 taatcgttct ctccttataa aaatttattc tttctcataa atggaaacgc ccgtttcgcc
      601 gtcgcactcg gcgaggcgca ccgcgacgat ttcgcgcagg gaggtgggga tgttcttgcc
      661 gacaaaatcc ggcttgatcg gaaactcggc gtggccgcgg tcgatgagcg aaaagagctg
      721 gatgcgtgcc ggacggccga gagagatgag cgcatcgata gctgcgcggg cggtgcgggt
      781 cgtaaaaata acgtcgtcac agagcacaac ggtcatcccc tttacatcaa agggaatgtg
      841 cgtgtccgaa acgacgggcg catcggcaac gagcgtgaga tcgtcccgat aaagcgtgat
      901 atccagtttt ccgaccggga cgcgggtgcc ctcaatcttt tcaatgtttt tcgccagacg
      961 ctcggcgaga gggatgccgc gggtcttgat gccgatgaga cagagatttt cggtgccgtg
     1021 attcttctcg atgatctggt gggcgatacg cacaagcgtc cgctccacgg cggcttcgtc
     1081 catgatctgc gctttgaagt tcatagcgtt ccatatcctt tccggcattt tggcaacaaa
     1141 aaaaccttga ccggcaaatc cggacaaggc gcagcgacac ggcataaaga taccgtgatc
     1201 ccatcttacg acgccttgcc ggcagctctg taccgactta aagaggtctt ttctggcgtg
     1261 tatattatca tgccccgcta catctgtcaa cggggaaatg cacaaataac cggatcaaag
     1321 cggggatatt ttctatggag cgcatcggct cggacggggg aaaggccgtt tagaggcttg
     1381 cattatccgt tgaatgtatg ataatataca ctgatgtatg ataatataca caggaaagat
     1441 tctctatttc aattccgagg aaaggacacg gacaacttat gggagtttat gtagtcaccg
     1501 gcggctccgg cggtatcggc ggcaagacgg tagaaattct gcgcagccag ggacatgaga
     1561 cgatcaacgt cgatttgaaa ggcggggata tcaacgccaa cattgccacc aaggagggac
     1621 ggcaggatgt gatcgataag atccacgagc ggcaccccga ggggatcgac ggcctgatct
     1681 gcgtggccgg cgtcgctggt acctgcggcg atatgaagct gatgttctcg ctgaactatt
     1741 tcggcgtcac cggtctggcc tacggtatct atgacctgct caaaaagaag ggcggcagct
     1801 gcgtggtcat cgtatcgaac accatctcgc aggggggcgt gcacatggat ctctgcgata
     1861 tgctcaacta tgcctgcaac caggaccgga acgaagcccg cattctctcc attctggagc
     1921 agtatgacgg ctccaatatg accatggcgc agggtctgta cgccacaacc aaatacgcgc
     1981 tggcccgctg ggtacggcgc atctccgcca gctggggcgc caacggcgtg cgcatcaacg
     2041 cggtggctcc cggcaacgtc cggacgcctc tcaccgccgc catgggcgac cgcgctacgg
     2101 cggcgctggc gggacttccg attcctatca actatcagac cggcgaccag ctgctcgacc
     2161 ccgtggac
//
LOCUS       DAWAAE010000208         2129 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_17446_length_2129_cov_2.216972, whole genome shotgun sequence.
ACCESSION   DAWAAE010000208 DAWAAE010000000
VERSION     DAWAAE010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17446_length_2129_cov_2.216972"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          467 a          585 c          613 g          464 t
ORIGIN      
        1 acgatacaca gaccggcgct gcggaaaagc agggggatga ccatgagcgc ccaaaagaca
       61 aagatccaga tcgagcgcag gaagctcagc cagcggctcg agtgggtggg ggtgaagatg
      121 cccagctcgc gggcgttgcg cttcgccttg cgcagccaga gccggtagcc gagcagctca
      181 acgccgaaga ggaggatcat gacgaagatc atcagcatca tggtgaggtc gtagccgtcc
      241 gcgagaaagg cgaccggatc gcgccgcagc tgcgacacct gaagatagag catcgtcggc
      301 agcatgcagg cgaccgtgat cgtgttgctg agcacatagc ccttcttggc gcgctccatc
      361 gtttcgacct gcacgacggc ctcggtctcg atgggcacgg cgtcaaggtc ctcgttgtag
      421 aaaatgtgcg tgtcgtcgcg ctgcgtaacg atctgccagc ccgcgccggc gcagtagtcc
      481 caaaaggtca ggttctcgtg gtttggttcg tcgggacaaa agctgtcggt cttggggtag
      541 tagaccaccg cgaagcggag ctttttcggc tcgcagcggc ggtagcgcag ggcgtaggtg
      601 ccgacggatt caagcatcca gccgttggcg gccattttct ccaggtgccg ctcgagtccc
      661 gtgtggtcgt agaaggcgta gaggttcagt tcccgcttct tgttccatcg gtcagttcgc
      721 atggcttgtc gcctcccttc aaaagtcgct ggtaatcgtt catctgcgct gtcagacggt
      781 ccatctcgcc gtgcagccgc tcctcgcctt tttcggtgag aacgtagctg cggcggcggc
      841 cctcgcattt ggtctcgacg atgtagccct cggcggcaaa ctgctccaaa agattgtaga
      901 gcgtgccggg gccgacgctc acgcggccgc tcgtgagcag acggatgcgc tccatcagat
      961 ccatgccgca gcattcctcg cgcagacaga gaaggacgta aaacatctgc tcggtgagcg
     1021 tctgaaattt ttcgcgtgcc attgtaccgc ctccttttat cgatattcga tattcttggg
     1081 gagagtatac tatcgaatat cgatattgtc aagagaaaaa atcggagagc cgtcggcttt
     1141 ccgatttttc attcaggatt tgtcctcttc ccggcattca agaatatcgc cggcatcgca
     1201 gttgagcgcc tcgcagatcg ccgcgagcgt ggagaagcgg atcgccgtca ccttgttgtt
     1261 ctttatcttg gacagattca cgttggagat gccgacgcgc tctgccagct cgttcagcga
     1321 catttttcgc tcgaccatca tgcggtccag tctcagaatg atctttccca tttacacaag
     1381 cccttccaca tcttgttcca gctccacgcc gcgcgcaaag aactgcgtca gacagaggat
     1441 cacaagaccc atggagaagc cgtagagccg aacgctcgtt tcgacggtat cggtgccgag
     1501 gatcaggcgg cacacggtgc tcagcgcaag ccccgcgagc ggaatggaga tggcgaaaat
     1561 gccaatctcg cggagcatac ggatattatc cgcctgaaaa accgtgctgc tctctgaccg
     1621 cttgatgata aggtagagat tgcggaagat catcgccatg agggaaggaa gcacgacggc
     1681 tgcggcaaaa tatagaagca ttgcccgcat atcaatgccg ttcgcggtcg gtacgctgaa
     1741 ctcaaagccg aggacgctga gctccaatcg accggtacgg atcgatcccg ccagagcgtc
     1801 cgcgagaaat gtgcggtccg caaacgagta tattccggct gtgagcatga gagcggccgt
     1861 catccaatag ccgagctctg taaattttgt gatgatcttg gccgccttga tgatccaacg
     1921 gttccacttc atttttccga cctcctcaag atgcttgatg aggacagtat aaccgccgag
     1981 tattcgtttg tcaatatatt cttatcgttt tacgataaat tatatatgat tatcataaaa
     2041 tcatcacgcg gatataggca aagcccccga ccgcagccgg gggctttgag agggagatcg
     2101 ataaattgat tggacggtcg gtcactcca
//
LOCUS       DAWAAE010000209         2101 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_17738_length_2101_cov_3.428152, whole genome shotgun sequence.
ACCESSION   DAWAAE010000209 DAWAAE010000000
VERSION     DAWAAE010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_2101_cov_3.428152"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          687 c          621 g          362 t
ORIGIN      
        1 gcgctctcgt cgctctccgt cccgctcatg gagctggaga atacggcgtc ctttttcgcc
       61 agaaccggcg actacgcgct ctggctcctg cgcgagagca gcggcggcga cgatgtgagc
      121 gaggaagcgc gcgaaaatct gcgcgcgctt ggcgacacgg ccgcgcttct ctccggcaat
      181 ctggcgcagc tccgctccga tgtcgccgga ggtctcgtga gcacccgcgc cgccgccgcg
      241 atggacgacg cgctccccac ccttggcgac agctttctcg gcatggagca ggagtttccg
      301 gaaatgccga cgctcgtgta cgacgggccg ttttccgcct ccgttgccga gcgcgagccg
      361 cacatgataa aggacgcgcg cgaggtgacg caggacgccg cagcgctcgt cgcggcgggg
      421 tttctcggta tgcggagcaa tctcgtctcc gccgccggag agagcgaggg caagattccc
      481 gtataccgct tcaccggcgg ggactacact gtgagcgtca gccggaaggg cggatacgtc
      541 gtgcgcgccc tctcgcagcg cgcgccggtg cgctcggcca tcaccgtgga cgccgcgctt
      601 gaaaaggccg cggaattcct cgccgcgcac ggctgccggg gcatgaagga gagctatcat
      661 attctcaagg accacgtgct caccgtgacg tactgcgccg agcagaacgg cgtgatgtgc
      721 tatccggaca tggtaaagct cgccgtcgcc atggataccg gcgagatgct gcgctttgac
      781 gccgaggcct atctcacctc ccacgccgag cgggatctgc ccgaaccggc ggtgtcggag
      841 gaggacgccc gcgccatggc aggggagggg ctgacggtcc aaagcgaaaa gctcgccgtc
      901 atcccgacgt ccggcgcgga ggagatctac tgccgcgagc tcatctgcga aacggaggac
      961 gggcggcatt atcttctcta tgtgaacgcg atgaccggcg cgcaggagaa gattttgatc
     1021 cttcttgagg acgagagcgg cacgcttgca ctctgaaacc ccctcaaaac agtgggaaaa
     1081 cttgacagag gcggattcgt atagtaaaat acacacacta ttatggaata gtataaattc
     1141 cgaaagtatg cagttttatt atacaaagga ggtcatccta atgggcttca ttccggaaac
     1201 cacctgccgc cgctgcggcc agaaatactc cggactgcgc aaccgctgcc cgaactgcgg
     1261 cacgccccgg cagaatcagc cgaaccgcgt accgcatacg accgcatccg ccacgccgaa
     1321 caccgccgct tcccgccgcg ccggcgccga tatccgctgg cagctcatct tcggcggcat
     1381 tcttctcgtc gccgtgatcc tcgccgtcgt cgtgctcatc gcgggcggag ggaaggactc
     1441 cggccccagc tccggcaacc gcccgtccgt gacgaataac cccggcagcc agatcgtcta
     1501 cagcgcggcc gacctgccga cacccagccc ctcgcccatc ccgacgcccg aagccagccc
     1561 caccccggtc attgaacaaa tggcgatcgc attcctcaat aacagcgtca agaatcactc
     1621 tctcacgctg acgaacgccg gtgagatcac gatcgatctc gatctgaacg tcttcccgag
     1681 ctcggacgat ctgcccgtga cgtggagcag cagcaacgag aagatcctca ccgtggacga
     1741 ccgcggcatc gtgaccgttg tcggcgcctc gccgaacatc accgtccacg cggtgatcat
     1801 cgccgagtgc ggcggtctgc aggactatgt gacgatctac gtcccggcgt atcaggcggc
     1861 atatctcacg cagaacctgt acgatcccga aacctacgag caggacaacc tcgagtggga
     1921 cagcatcatc tacgccaagc cctcggccaa gccgggctga tcgacgcaga tttttccgaa
     1981 aaataacgca aaaacagttg acaaggggta ttatcttttg tataataccc cttgccttga
     2041 aaaattcgga cgattagctc agctggtatg agcatccgct tgacgtgcgg agggtcacag
     2101 g
//
LOCUS       DAWAAE010000210         2027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_18446_length_2027_cov_3.399087, whole genome shotgun sequence.
ACCESSION   DAWAAE010000210 DAWAAE010000000
VERSION     DAWAAE010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18446_length_2027_cov_3.399087"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          346 a          613 c          679 g          389 t
ORIGIN      
        1 gcgtagacgt gctcgtcctc atacaccttc gcggacggga tctccccggc gatgatcttg
       61 cagaaaaggc agtcgttcat aatagtgctc ctttcacata atgggcaaaa gccgccgcaa
      121 tgcggcggct tttgaaaaat tcagttcagc tttccggcga cgaaatcgtc cacggtagca
      181 agagcgtcgt cgagcttgag cgtatcggcg ccgccgccca tggcggaatc cggcttgccg
      241 ccgcccttgc cgccgcagat ggcggtgacg gagcggatga tatcgcccgc gcggacgccg
      301 gcagcgaccg ccgccttgcc gcacacgctg tggaaggtga tctttccgtc gtgcacggcg
      361 gcgagcaccg caacgatgct ctcgtccttg tcgcgcagga aatcgccgat cttgcggagg
      421 ttgtccggcg tatcgaccgg cacgacgccg gtgaacacgt gcagcgggcc gaccttcttg
      481 gagctggcga ggaagctctc ggcgttggag agcatttcgc ggccgcggaa cttttcgagc
      541 tcggagcgca gctcgcggat ctcgctcgtg cgcgcctcgg cgcgggtgag cagctcctcc
      601 ggcttggcct tgagtcgctc ggagagcgtc atgagcagac gggtgttgtt ctccatgaat
      661 ttgagcgttt cctggccggt gatggcctcg atacggcgca cgccgctcgc aacggaaaac
      721 tcactcacga tgcgcagcga gccgaccttc gcggtgttgt cgaggtgcgt gccgccgcag
      781 aactccacgg agtaaccatc gcccatatcg acgacgcgga caacgtcgcc gtacttttcg
      841 ccgaagagcg ccattgcgcc gatactgcgt gcctccgcga cgggcatctc cttcacggtg
      901 atggggaagc cttcgagcac ggcctcgttc accatgcgct cgacggaggc gatctcctcg
      961 ggcttcatgg cggagaagtg ggtaaagtca aagcggagac ggtccggctc aacgagcgaa
     1021 ccggcctgat ggacatggtc gccgagaacg gtgcgcagcg ccttgtgcag caggtgggtg
     1081 gcgctgtggg cgcgcatgat cgccttgcgg cggccggtat cgacgcaggc gttcaccttg
     1141 tcgccgacgg cgagattgcc ggagaccatc acaccgtggt gcagcacctt gccggccttg
     1201 ttcttctgca catcgtgcac ctcaaagacg gcgtccttga gaatgagcag gccatggtcg
     1261 gcgctctggc cgcccatttc ggcgtagaac ggtgttttat caagaacgat gctcgcgtgc
     1321 acgccggagc cgacgcgctc gcgcagctcg tcctcggcaa cgatggcgag gatggtgcag
     1381 tcgtcgtgca gacgctcata gccggtgaac ttggtgggga gattgtccag gccgagatcg
     1441 agtcccgccc aggcggtctc atcggagctg cgggcggcgc gggcgcgctc gcgctgttcc
     1501 ttcatcaggg tctggaacgc gggctcatcg accgtcatac cctcgtccgc cgccatttcc
     1561 ttcgtgagat ccagcgggaa gccgtaggta tcgtagagct tgaaggcgtc ggcgccggag
     1621 aacacggtct cgcccttttc cttgtgggag gcgagcatac cggcgaagat agccatgccg
     1681 ccgtcgatcg tgcgggagaa gttttcctcc tcggagcgga tgacgccggt gatgtactcc
     1741 tgccgctcgg taagctcggg gtaagcgccg cggttctcgt ggatgacggt atcgcagact
     1801 ttataaagga acggctcctt ctcgccgagc agacgtccgt ggcgggcggc gcggcggagc
     1861 agacggcgca gcacgtagcc gcggccctcg ttggagggca gaacgccgtc gcagatcata
     1921 aaggtcgagg aacggatgtg gtcggtaatg atgcggagag acacgtcctt cgcgttcgtc
     1981 tcgccgtagt gcgcgccggt gatctcgctc accttgtggg tgatgtt
//
LOCUS       DAWAAE010000211         2008 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_18654_length_2008_cov_2.932924, whole genome shotgun sequence.
ACCESSION   DAWAAE010000211 DAWAAE010000000
VERSION     DAWAAE010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18654_length_2008_cov_2.932924"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          408 a          576 c          522 g          502 t
ORIGIN      
        1 gttatagctc agtcggtaga gcgcatcctt ggtaaggatg aggtccccag ttcgaatctg
       61 ggtaacagct ccaaaaaagc ctgtaatcct aaggattaca ggctttttta tattttccga
      121 accagttcga tgcagcaatc gatcaagctt gcccggacgg ttttttgggt gttccaaccg
      181 cggggatccc acgccgtgcc gctcacatca tccccaacat acaggagttg tacgagcgga
      241 atattatagt actttgctac agcaaaaaac gctgccgttt ccatctcgac cgtcacgcag
      301 ccctcgtccc tccggcgcgc gatcatttcg ggtgtttcgc ggtacatggc atccgtcgtc
      361 catgtttttc cgagcacaaa cggcactccc atctcggaca acgccctctg cgctttttcc
      421 agcgcttctt tatgacacga gatttcccgt gaaggggcaa tgtagtgaaa tgatgtgccc
      481 tcgtcccgta cggccgaagc cgggagaatg atctcgccaa gcgtgctccc ctgtgtcagt
      541 gcccccgcgg cgccgcatac cacaaacgtt tcgcatccca tggcatgcag ctcttcgatc
      601 gttccggcgg caccaggtgc cgtgctgaac ggcatggtga tgcaaagagg gttctctccg
      661 ttttcatatt tgtagatcgg tatatcgaga acctccgagt gaagttggcc gatcacaggc
      721 agtttgtgct cccctgcgaa tttttccagt tctttccgaa aaaatgtaat aatgcatttt
      781 ttcggaagac atttatccag aaaatctgtc gggtgaataa ttggatctct ggcggtatcg
      841 tattcgcaga cgggatattc atgtgtgatt atcatgccgt cttttccccc tatgtattgc
      901 ataagcgatt aagatcattt tatcattgcc tcgcgtttat tgcaatccgc agtcaaacgc
      961 cgcccgaagc gtgcccacgc ttgacagcgc cccttgccgg ggatacactg atgacatcac
     1021 cggcaggagg aaaacctatg cccctcacag agaaaaagcc gctcagctcc aacgccataa
     1081 agctcattgc cattgccgcg atgaccgtcg accacatcgc gtgggcggtg tttccagggt
     1141 atccgaagga atttctcccc ctgctcatgc atctcatcgg gcgcatcacc tgcccgatca
     1201 tgtgctactg catcgccgag gggtatcact acacgcggaa catccgcaag tataccgcgc
     1261 ggctcttcat tttcgcgctg atctcgcatt tctgctacat atattcctcg cacgatttca
     1321 tcgactggca ctcctttatc ccgttctact acggcggcgc gctcaaccag acgagcgtta
     1381 tgtggtcgct tgcgtggggt cttgtgatgc tgcgcgtcgc ggaaagcaaa aagatcacgt
     1441 ccggcgcggt gcgggtgctg ctcattctgc tcatctgcct tgtgagcttc ccgagcgact
     1501 ggagctgcat cgcctcgctc tgcgtcatgg cgttcggcac gaaccgcggc gagctcaaaa
     1561 agcagggctt ctggcttctt ttctatgtcg cgctctatgc ggtcgtatat ttctttgcca
     1621 tcgaccgcgt gtacggtatt ttgcagatgg gcgttgcgct ctcgctgccg gttttgcggc
     1681 tctataacgg gcagcgcggc aaaagccccg ccgtgaaccg gtttatgaaa tggttctttt
     1741 atctttatta ccccgcgcat ctgctcctta tcggtctgct gatccacgcc tgactgggag
     1801 actaaaacta taaagagttt gggaggtagt tacgatggat gtaaacgcgc tttaccggct
     1861 gtggctggaa aaggccgcca gacacccggc gctccgcgag gagctggaag ccatccgcgg
     1921 cgacgatgcc gccatcgagg accggtttta ccgtgaactg gaattcggca ccggcggact
     1981 gcgcggcgtg ctcggcgcgg gcacgaac
//
LOCUS       DAWAAE010000212         1842 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_20546_length_1842_cov_3.302742, whole genome shotgun sequence.
ACCESSION   DAWAAE010000212 DAWAAE010000000
VERSION     DAWAAE010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20546_length_1842_cov_3.302742"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1224..1233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          438 a          546 c          553 g          295 t
ORIGIN      
        1 ggcaagcgcg agcgagagcg cgatctccgc ggcggtgccg gatgccgaga gcgccgcgca
       61 cacggcggaa aggtcgccgc ggctgcacgg cgcgagcaga agcgcggaaa gccccatgcg
      121 cagaagctgt gcgaaaaact gggagagcgc cgttttccac acgcgcccga cggcggtgaa
      181 gtacccggag atggcgctcg agagcgcaag cgccggaagg gagggcgccg cggcgtacag
      241 cgcgcgtacc gcgcgggcgt ccccgatcca gagaaagcca atgggctcgg caagaaaaaa
      301 gagaatggcc gccgcgccca gcccgaaaaa gagcgcgtag gcgcagcacc ggcgcatcac
      361 ggcgcagacg ctcccgctgc ggcactgccc cagctcctcg gcaagcagcc gcgtgacgga
      421 aaaccggatg ccggacaccg cgagcgttat gaaaagaaag ccgacgcttc cgataagctg
      481 gaaaagcccg atgccggcgg gaccgatatg cgcggccagc cacacctgcc agagcatcga
      541 aagcgactgc atgacgagag acgagaccgt cagcagcgcg gcgtttttcc ggatcgatct
      601 ccaaagcccc ataatgacgc ctcccctccg tgaaagagtc tatgcgcgcc gcggcgcgcg
      661 atagaagaaa agaaaaaacg gcgtctgccg gggcggttta tgcatctctt ccaatatagc
      721 ttgtcggaaa aacaacgaac cgtccgaaaa cgacgcacca aaatgtcgaa aacgggcggt
      781 tctgacggca gaatacacaa ataagaaaga tatagttgca gaataaatca acgcttgtta
      841 taatatttat agtatggatt gatcataccc cagcgcttcc ccgcataaaa aaaggaggag
      901 aaagatcgca tgaacgaaaa gtacagttcc ctgtttaccc cgtggaagat cggcaactgc
      961 gagatcaaaa accgcattgt tcaggtgtcc atgggcggca catccctctt cggctggctg
     1021 gagccctgcc acttcgataa ggaagcggcc taccacattc tcaaccgcgc ccagaacaac
     1081 gtcggtctgg tgctgcccgg catgcagtgc gtgcgcgaca cgatgggcat ccccggccgt
     1141 cactggctgt atcagaacaa gcgcatgttc cgcgagctcg ccgagtggat gcccgaattc
     1201 cacaagacgg gtgcgcagct cttnnnnnnn nnngctcttc atccagctct ctgccggcat
     1261 gggccgcagc atggcgatca accagctgat ggtcgatatg ctccagaaga aggcgcttgg
     1321 cgccgtcgcc aagggcgttc tggacgtcga ttatgtctgc gcgtcctcct ccgagacccc
     1381 caaccgctgg tatccggagg tcaagtcccg cgcgctgaca aaggaagaga tccatgaaat
     1441 gatcgaagcc ttcggcaaga ccgcgaagat gcttatggat gccggcgttg acggtgtgga
     1501 gatccacgcc gttcacgagg gctacctcct tgaccagttt gccattgcca acatgaacta
     1561 ccgtaccgac gagtacggcg gatcgttcga gaaccgctac cgctttgccg ttgagatcgt
     1621 gcaggagatc aagaagctgt gcggcaagga cttccccgtg tcgctgcgct actccgtcgt
     1681 ctccaagacg aagggcttcg gccagggtgc cgtccccggc gaggagtttg ttgagttcgg
     1741 ccgcgatatg gccgagtctg aaaaggccat caagtatctc gaggacgccg gctacgacat
     1801 gttcaactgc gacaacggca cctacgacgc ctggtactgg gc
//
LOCUS       DAWAAE010000213         1738 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_21924_length_1738_cov_2.964349, whole genome shotgun sequence.
ACCESSION   DAWAAE010000213 DAWAAE010000000
VERSION     DAWAAE010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21924_length_1738_cov_2.964349"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          545 c          543 g          294 t
ORIGIN      
        1 atcgacacgt tccgctcctc cggcgcgggc gggcagcaca tcaacaagac ctcgagcgcc
       61 atccgcgtga cgcacctgcc gaccggcacg gtcgtggagt gtcaggacga gcgcagccag
      121 tataaaaaca aggcgcgcgc gctgagcgtg ctcgcctcgc ggctcgcgca ggcggaaaag
      181 gagaagcagg acgccgccgt ttccgcgcag cgaagatcgc aggtcggctc cggcatgcgc
      241 aacgagcgca tccgcaccta caatttcccg caggggcgcg tgaccgatca ccgcatcggt
      301 ctgacgcttt ataagctcga cgccgtgatg aacggcgatc tggacgagct catcgacgcg
      361 ctgcttaccg cgcgccgtgc cgaacagctc caaaattccg aaaacggagg catgtaagag
      421 catggaaacc cgtgtttata ccgaagagaa cgatatcccc gtcgccgcgg cgtcgctgcg
      481 gctcggcggt ctcgtcgccg tgccgacgga gacggtatac ggtctctgcg ccaacggtct
      541 cgacgccggg gcggtcgcgc atctctatga agtcaagggc cgcccggagg taaagccgct
      601 ctcgctcatg gtggcgggcc cggcggagat cgaaaagtac gcggaatata tccccccggc
      661 cgcgtttatt ctcaccgcgc ggttctggcc cggcccgctc acgctcgtgc tggaggcgaa
      721 aaaggacgtt gtcccctcca tcgtgcgcgc gggcaaggac accgtcggac tgcgctgccc
      781 ggcgcacccg ctcacgctct cgctgctcga acgcgcccgg ctgcctcttg ccgggccttc
      841 ggcgaaccct tcgggtgctc ccagccctac gaccgcgcag caggtgctcg cctactttga
      901 cgggcagatt gaaggcgtga tcgacggcgg cgaatgcacg ctctcacgcg agtccacgat
      961 cctcgatctg acgcagaagc ccttcaaaat tctgcgtgag ggcgcgctgg caaaggaaga
     1021 gatcgaaaac gcgctcctct ccggcgtgcg gctcatcggc gtgacgggcg gctccggcag
     1081 cggcaagacg accgtaacgc agtttctcgc cgcgcagggc gtgctcgctc tcgactgcga
     1141 taaaatatac cacgagcttc tcgaaacgaa cgcggatatg ctcgcggaga ttgaggcgcg
     1201 cttccccgcc gttttcaaaa acggaaaact cgaccggaag atgctcggcg cgatcgtctt
     1261 tgccgtgccg gaggcgctgc gcgatctcaa tgccatcacg caccgctatg tccgcgagga
     1321 tatttaccgc cgtctgcgcg aatacgtctg gcagggcgga accgttgccg ttgtggacgc
     1381 catcgcgctt tttgaaagcg gcatttccga ggactgtgtg ttcaccgtgg gcgtcaccgc
     1441 cccgggcgac gagcgctgcg cgcgcatcat ggcgcgcgac ggcatcgacg cggactacgc
     1501 gctcgcgcgc attgaggcgc agccggacga cgagtattat gaaatgcgct gcgattatct
     1561 gctgcgcaac gacggcacgc cggaagaact gaaagccaaa tgccgggagc ttttcggcat
     1621 cggttaataa aagacagtat aaaaaagtac agaaaggata gatgttttta tggaagaaaa
     1681 gaatgaactg gacgctctgc gggaaaagct cctttacaag cagaagcagg gctacgat
//
LOCUS       DAWAAE010000214         1609 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_24014_length_1609_cov_2.940798, whole genome shotgun sequence.
ACCESSION   DAWAAE010000214 DAWAAE010000000
VERSION     DAWAAE010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24014_length_1609_cov_2.940798"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          319 a          520 c          480 g          290 t
ORIGIN      
        1 ggacgggacg gctggctttt ctacgcacca acgatccatg attataccgg cgacgcgccc
       61 atgacggcgc gcgagctcta ctgcgcggcg cgcacattgt atcttctgca ggagtacgcg
      121 gaaaaccgcg gcggagactt cctcttcacc gccgcgccga acaaaaatac gctctatccg
      181 gagtacatgc cggcgcggac gcgtctcggc agcgtttccg atatggacgc gctctacgcg
      241 ctgctcgacg agatgggtgt ttcgtatctc gatctgcgcg atgtattctc acaggaagac
      301 gagccgctct atttcaaaac ggacagccac tggaacgcga agggcgcggc gctcgccgcc
      361 gacgcactgc tcgctgctct tggccgcgag agcgattatt tttccggcac cgtatccgcc
      421 ggaaacaccc accgcggcga tctttacgaa atgctctatc ccgcgggcaa agagctggag
      481 gaagatttcg catatgcccc cggtttttcg ttcaccgcga acaccgataa ccccgaccgc
      541 gtgacgatca cgaccgaaag cggcgtcggg accggcgcgc ttctctgcta ccgcgattcg
      601 ttcgggcgca atctctaccc gtatctcgcc gagagcttcg cctcggcgga gttttcccgc
      661 cggaacgagt ataccgccgc gacgcttccg ggaggcggca cgctcgtgat cgagctcgtc
      721 gagcgcaatc tccggtatct cgtggagtac gactcgctcg cccccgcacc ggagcgggac
      781 gcctcgctcg tcgagacggc tgccctgggc gatgggagag ccgttttgac ggagagcgcc
      841 ggtacggaag ggtacacgct cttttccggc acatgggacg gcgtgacgcc ggacgatacg
      901 tccaacgtct atgtcctctc cggcggcgcg ctctatgagg ctgtgccgcg tccggacggg
      961 tttattgtat cgctgccggt cggcagcacg atcgacgccg tatatgcggc ggcgggagga
     1021 aacttttccc gtctatccgc cgtctatgct atagactgat aaaaaaacca aaggagacaa
     1081 aactacatga aaaagatcct tgctctgctg cttgccacgc tgatggcgct cgctctcgcc
     1141 ggctgcggcg gaaacgccga gacccctgcc cccaccgaaa cgcccgcaac ggaagccccc
     1201 gctgcgaccg aagctccggc gtctgaagag cctgccgtga ccgaagcccc cgccgaaccg
     1261 gagggagacg gcgcagaaga ccgtgtggcc ggtatgatcg agctggtgct ctcgctcaag
     1321 ggcaagccgg tacaggagct ctacgacgcc atcggcatgc cgaccggcga agcggagtac
     1381 gcctcgagct gccttgtcac cggcggcaag gacggcgtgc tgccctacga cggcttcacc
     1441 gtgtacaccc tcgtgcagcc cgacggcacc gagaccgtgt acgacgtcga aaagaactga
     1501 accatttcag acaaagaagg cgggatttgt cccgccttct ttgccggaac gtcaaagaag
     1561 cctcgcagag tttgcgcccg caggcgcaaa taaagtttta accattttc
//
LOCUS       DAWAAE010000215         1552 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_25056_length_1552_cov_3.864395, whole genome shotgun sequence.
ACCESSION   DAWAAE010000215 DAWAAE010000000
VERSION     DAWAAE010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25056_length_1552_cov_3.864395"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          298 a          452 c          439 g          363 t
ORIGIN      
        1 tagttgagcg gtctatgcaa gaccacaaga tattgtatca catcttctcg gaaaaaacca
       61 gaggaaaatg acgaaaaaaa ggaaaaagcc ttgcccatca tagcggcctt tgatataata
      121 ctcattcacg ataatcaaga aaaagcgagg aaacggatct atggaggaaa caaacagcct
      181 ggcccggtcc cggcgcatgg gcgaaatgcc ggagggaaag ctgctgctgt caatggcagt
      241 gcccatgatg ctctcgatgc tggtgcaggc tctctataat attgtggaca gcgtatatgt
      301 ggcgaaggtg tcggaggact gcctttcggc gctctcgctc gcgttcccgg cgcagaacat
      361 tctcatcggt cttgccaccg gcacgggcgt cggcgtttcg acgatgctct cccgcgctct
      421 cggcagccgg gacaacaagc tcgccaaccg cgtggcggga aacagcctgt tcctctgcgt
      481 gtgctgctgg attattatgg cgatctttgg tattttcttt gccgacgcct tcatccactc
      541 gcagacggat atcgagtcca tccgcagcta cggagattcg tatctgcgca tcgttacgat
      601 cggctccgta ttcgtgtatt ttgagatcaa ctttgagcgc cttctgcagt ccacgggcca
      661 gacgaagctc tccatgtggg cgcagataat cggcgcggtg gcaaacatca ttcttgaccc
      721 gttctttatc tacggctggt gcggcctgcc cgcgatgggc accaccggcg ccgccatcgc
      781 cacggtcatc ggccaggcgt tcggcgcgct ggcgggcttt ctgtttcacc tcaaacataa
      841 taaagaagtc aatatccgtc tttccgactg ccgcccggac gggcgcatca tcctcgacat
      901 ctatcgcatc ggctttccgt ccatcctcat ggtcggtatc ggttccctta cgaactatct
      961 catgaaccgc atcctcatgg cgttcacgtc cacggcggtc gcgttgtacg gcgcatactt
     1021 caagctgcag agcttcttct tcatgccggt gttcggactg aataacggca ttgtccccat
     1081 cgtcggctac aactacggcg cgcggaaaaa ggaacgcatt taccggacga tccgctacgg
     1141 cgtgctgtat gccgtgtgca tccttgctgt cggcctgctg ctctttgaag cgatccccgg
     1201 cgtgctgctg caggcgttct cgccgtcgga gcacatgctt gccatcggca ttcccgcgct
     1261 gcgcaagatc gccatccatt tcccgatcgc ggcgttctgc atcgtgggcg ggtcggtctg
     1321 tcaggcgctc ggcaagagtc tctattcgtt cttcacgtcg gtgctgcgtc aggtcgtggc
     1381 gctcatcccg gcggcgtatc ttctgtcgct gacagggaac gtcggcaacg tatggtggtg
     1441 cttcccgatc gcggaggtcg tatcggcgtg cgcaacggcg tttttcctgc gccgcgcgct
     1501 gcgaaatatg gaccgggagc tgacgctcgc ggaggggcaa tgaaggaatt ta
//
LOCUS       DAWAAE010000216         1515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate gSpreadC_ID_527
            NODE_25768_length_1515_cov_2.420548, whole genome shotgun sequence.
ACCESSION   DAWAAE010000216 DAWAAE010000000
VERSION     DAWAAE010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993028
            Sequence Read Archive: SRR3736999
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (human gut metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25768_length_1515_cov_2.420548"
                     /isolate="gSpreadC_ID_527"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          216 a          514 c          465 g          320 t
ORIGIN      
        1 ctcgccctcg tcccagcgga agaaggtgat gatctcgccg ttcgtatcgc gccagttgcc
       61 gtccgcctga cgctccgcgc cgagccagac atagttgcat ttggcgctcg tgcatacgcg
      121 gacgatctcg ttatactcgt cctccgtcaa gggcaccgcc agcgtgccgc cgagctcctc
      181 gcagcgctgc tgcgcctgcg tccagctcac gttttcggca tagacgacat agcgcgggcc
      241 cttcggcgtg ggcgacggcg tcgggtccgg cgtagcggtc ggcgtcgggg tggggatctg
      301 cgtcggttcg ggcgtgggag ttacctgcac ctggggttcc ttattgttcg ccgtgccgct
      361 ggcgtatgcc gcaacgaccg cgccgacaac aacgatcgcg gcgagcagcg cccagagcca
      421 gcggaagctc ttccgccgct gcggacggcg cccggcgggg atctccccgt cgtcgtaata
      481 tgtacggcct cttccctgcc catcgcgccg gtcgatcgcc ggagcgtccg ccgagcgcat
      541 cagaagaccc gacatggcaa gggagatgtc caccgccgat ccggcggcgt cgcatccgcc
      601 gagagcgtcc ttcagctcct gcacatcgtg ccagcgctcc tcgatgcgaa acgccagagc
      661 acgcagaatg ataccggcca gctcggcatc cgcactcgca ggcggctgga tctcctcgtt
      721 gctcatgcgc ttctgcagcg cgctggcccg ggcgttcggc gtgatcgcgc cggataccgg
      781 ccagaacggg agacggccgt agttatagag agaatagagg atcagtccga tggaatacac
      841 atccgccgtc ggggtgcgga tgccgtccca gaacagctcc ggcgccatat actcgagctc
      901 ctgcggcgta aactcgccga ccgtgtgctg gagcgtcccg ccgagatagg ccttatctcc
      961 gcggacggag atgttgtccg ggcggatgcc gccgtggaaa tggtcccggc cgacgctgta
     1021 gagcaccgcg tcgcagagcg agatggcaag gccgacaccg ccgaggccgt ccgccttggc
     1081 gctctctgcc ttcttctccg ccgcaggcgc agtcttcggc gtttccgtgg ctggcttgtc
     1141 ttccccggca ggctccgccg tttccggcgc ttcctccttc ggagtttcct cctttggcgt
     1201 ttccgcttcc ggcgcgtttt cctccttcac ggcttccgcc ggttccgacg gtttttccgc
     1261 tttcggcgct tcttccgtat tcggcgtttc ctccggcgcg gcggtttctt ccgccgccgg
     1321 gatatccgcg tccgccgccg tttccggcgc ggtatcgttc tgcgcagaag cgggctgcgg
     1381 tctttcttcg ggcagacccg tatctttttc gttgtccgtc atgctatcac ttcccgcatt
     1441 ttattcttgg aaattatacc atgagagcaa atcgatgtca atttttctta acgcaaaagc
     1501 cgtccctccg aagag
//
